LOCUS       DAOPUW010000001        12923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_4932_length_12923_cov_3.131489, whole genome shotgun sequence.
ACCESSION   DAOPUW010000001 DAOPUW010000000
VERSION     DAOPUW010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 12923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12923
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4932_length_12923_cov_3.131489"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..2086)
                     /gene="rho"
                     /locus_tag="PKJ43_00005"
     CDS             complement(122..2086)
                     /gene="rho"
                     /locus_tag="PKJ43_00005"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNZ70966.1"
                     /translation="MYDIIELNQMLVNDLREIAKNLQIKKPEKLKKQELVYKILDNQA
                     ILASEVKQKQEKPAQPSKQHSEITDSPKKGESQDSIFKRKRIRILRTSEEKVQITSKD
                     NQQVKPEEKIEQPSEQREEPIIEQKEENSPRQVQKEIPFFKPRQQNENSDKKFFSKKP
                     QNDREQRNRNQDNSKEKFVDKNHSSQPYPSDKPKQNYVVGKPDMILPEIEPEDLTDKD
                     DFVVAEKAPIAAPVEKKVEVSLPTDRNYEKRPERQQVDPGNVNPNARQNYRNAKQQDK
                     TIFDFEGFITNSGVLEIMQDGYGFLRSSDYNYLNSPDDIYVSQSQIKLFGLKTGDTVK
                     GTIRPPKEGEKYFPLIKVLEINGRAPEYVRDRVPFDHLTPLFPNEKFNLTGKGDSSLS
                     VRIVDLFAPIGKGQRGLIVAQPKTGKTVLLKEIANAIAANHPEVYMIVLLIDERPEEV
                     TDMARSVNAEVVSSTFDEPAERHVRVANIVLEKAKRLTECGHDVVILLDSITRLARAY
                     NTVQPASGKVLSGGVDANALHKPKRFFGAARNIEEGGSLTILATALTETGSKMDDVIF
                     EEFKGTGNMELQLDRKLSNKRIFPAVDVTLSSTRRDDLLIHKAMLDKIWILRNYLADM
                     NSLEAMEFLRDRLLKTTTNEEFIISMNSGN"
     gene            2542..3108
                     /locus_tag="PKJ43_00010"
     CDS             2542..3108
                     /locus_tag="PKJ43_00010"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNZ70967.1"
                     /translation="MNIAENKMVTLSYVLRADSVNGEIIEQTTNDSPLRFVFGLGQML
                     PAFESNLSGLKQGDDFEMTLSAREAYGELDESAIIDLDKEVFFIDGQFDEERFMPGTQ
                     VPMQTNTGQRLNGIVIEVTETSVKMDFNHPLAGTDLHFTGKVIEVRDATEEELTPMGC
                     GCGCGSDCGSDHDCSSESGHCGSGGCGC"
     gene            3124..4197
                     /gene="aroC"
                     /locus_tag="PKJ43_00015"
     CDS             3124..4197
                     /gene="aroC"
                     /locus_tag="PKJ43_00015"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNZ70968.1"
                     /translation="MNSFVNIFRLTTFGESHGSGIGGIIDGCPAGLVLDLEKIQYELD
                     RRKPGQSKITTQRNETDTVEFLSGIFEGTTTGTPIGFFVKNKDHHSSDYDSLKDIFRP
                     SHADYTYTQKYGLRDHRGGGRSSARETIARVVGGAIAKQLLMLENISIQAFVHSVGEI
                     TLDKSYFELDLTKTEDNIVRCPDVKTAEAMIARIEEAGRNHDTVGGTIQCVIKGVPAG
                     LGAPVFNKLHADLGFAMLGINAVKGFEYGSGFDGTKLSGSEHNDIFVTEGGKVKTITN
                     HSGGIQGGISNGQDIYFNVAFKPIATLLKEQKTIDNKLEETTINPKGRHDACVLPRAV
                     PIVESMAALVLADHLLMSKLSKL"
     gene            4254..5120
                     /locus_tag="PKJ43_00020"
     CDS             4254..5120
                     /locus_tag="PKJ43_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNZ70969.1"
                     /translation="MRQLKITKQVTNRETLSLDKYLHEIGKVELLTPDKEVELAKRIK
                     KGDREAFEKLIKANLRFVVSVAKQYQNQGLGLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQSLAEQARIVRLPLNKIGSINKINKTFNQLEQEFQREPTAKEISE
                     ILDLTPDVIEESMRASGHHLSMDAPIYEEEASNMYDVLLNMDTPSPDKGLLNSSLQLE
                     IERSLATLGERESEILRYYFGLNGTRPHTLEEIGEVFGLTRERVRQIKEKAIKKLKNN
                     YRNKLLKSYLGE"
     gene            5225..5875
                     /gene="rpe"
                     /locus_tag="PKJ43_00025"
     CDS             5225..5875
                     /gene="rpe"
                     /locus_tag="PKJ43_00025"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNZ70970.1"
                     /translation="MTRFIAPSILSANFLDLRKDIEMINQSNAHWIHYDVMDGVFVPN
                     ISFGLPILKQISSIAQKPLDVHLMIANPDQYITAFKDAGAANITVHIETCPHLNRTIN
                     FIKEQGLMASVCLNPHTPIEMLEEIIQDIDMVLIMSVNPGFGGQLFIESTYRKIGKLK
                     KMILDKNLKTLIEVDGGVNFETGKKLFEAGANVLVSGSFIFHSDNPTKRIDDLLSV"
     gene            5887..6615
                     /locus_tag="PKJ43_00030"
     CDS             5887..6615
                     /locus_tag="PKJ43_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ70971.1"
                     /translation="MEDNNDIYDNIKDVINSIPDNYKILEETINIDTQKEYYESSKGI
                     SIDPALDIPEEMINQLNEGKFSSTEDCKILLQKLALLDSVEAYRTIEKFCLVAPPEIK
                     EWAILAKQQSRMVMQCSLLDEQQVFISTGLGGKNDKLRYLLIFPFSKKQVSKFQLDAL
                     EKELLYFLPKNEGEVEEFEIQKKYATSLILLPLKAPVNEIIQEILAECNQFGNFLSHN
                     AMITNMKKFSHEEISAFIEEEEEK"
     gene            6707..6779
                     /locus_tag="PKJ43_00035"
     tRNA            6707..6779
                     /locus_tag="PKJ43_00035"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6740..6742,aa:Arg,seq:acg)
     gene            complement(6848..7927)
                     /gene="buk"
                     /locus_tag="PKJ43_00040"
     CDS             complement(6848..7927)
                     /gene="buk"
                     /locus_tag="PKJ43_00040"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNZ70972.1"
                     /translation="MSAPKVLVINPGSTSTKIAVYQNETCLFEQTIKHYLDELVLYPT
                     ILSQLEFRYDLIVGNLEIAGISLNDISIVMGRGGLTYPLESGVYEVDDRMLTHVRKGV
                     MGQHASNLGPLLAHAIAKIIPGAHAYIADPVVTDELEPVARVSGHPRFERRSIFHALN
                     HKAVGRLYAQSIGKKYEEINLIVAHMGGGVSVGAHRKGRVVDVNNALDGEGPFSPERS
                     GTLPAGQLIEACFCGNFIEDEVRRMVTGEGGLVAYLNTNSMKEIVDFAEDGDEECRFY
                     VEAFVYQVAKAIGEMAVVLEGEVDAILLTGGIAYSSLIARSVTEKIKFISTVVVYKGE
                     DELASMAMNAQLMLSGELVPKKYPH"
     gene            complement(7939..8844)
                     /locus_tag="PKJ43_00045"
     CDS             complement(7939..8844)
                     /locus_tag="PKJ43_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNZ70973.1"
                     /translation="MEKIGKLEDIFNGLKSKPVKSMVVANGVDSHSIEAVGEAVRRGW
                     IKGILTGNRKIIEEQCLLAGFESHIFEIIDCPDEDDAARMAVELASKGKADILMKGLI
                     STDRFMKALLDKTVGLLPKGNLLTHITVMQTPGYDKLLIVSDVAIIPLPTVEQKAIIV
                     QYLISTAHRIGIEKPLVAFVAATEQVISKMPATTDAVLLKQRWLNGDFPGSVCDGPMG
                     LDLALDKEAAEIKNFNSPVAGNADCLLFPNIESGNVFYKANTKLFNASIAAMLVGASV
                     PVVLSSRGDSVETKLNSIALAAVVC"
     gene            9105..9773
                     /locus_tag="PKJ43_00050"
     CDS             9105..9773
                     /locus_tag="PKJ43_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNZ70974.1"
                     /translation="MEEYVDIHTHHVNIEWEQLAIHNYDLPLNEIPSVQYFSAGWHPW
                     HLNGFSADECKSEFKRLASYKNFVAVGECGLDRKKGNNWDEQILAFETQLEIAKEYKK
                     PLILHAVKSYSDLLQLLKMHQFENKVLIHGFIGNEQVIMQFSTFDTYFSFGKSIFNDH
                     SKSIELLKTIAPEKIFFETDDSNFSISDIYLRAATIRQVSVEELSRITKNNFRKFIGD
                     ELVR"
     gene            9757..10482
                     /locus_tag="PKJ43_00055"
     CDS             9757..10482
                     /locus_tag="PKJ43_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNZ70975.1"
                     /translation="MNWLDRTELLIGPQNIEKLKSAHILVVGLGGVGAYAAEMLCRAG
                     IGQLTIVDGDNVELTNINRQLPALHSNLEKRKTSVMAERLKDINPDIELTIIDEYIRD
                     ERMIEILDGNFDYVVDAIDTLAPKTFLIYHSVQKGLRIVSSMGSGGKFNPELVKSSDI
                     SKSYNCNLARMLRKRLSKLGIKKGVKVVFSPEEVDEKAIRLEEGTNKKSTVGTISYMP
                     PLFGCHIASVVIRDLVTLEQPSE"
     gene            complement(10488..12431)
                     /locus_tag="PKJ43_00060"
     CDS             complement(10488..12431)
                     /locus_tag="PKJ43_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNZ70976.1"
                     /translation="MRRNRLVPFFPYLCIFLVTLVAFIFMFSSCKTAKTGTRKSISLV
                     EQAKVAILSGDYLEAENLILKAIQSDTTAITPYLLLSDISEELKKTEQQKFALHKALS
                     IDSLYYKAAYKLLGGLYFTEGNYKEALNFYLFYQPYAKAGDSSFVNNRINSARFSIQS
                     IAENRQVLIDKPGEIINTSRQEYWPGISTNDSALYFTRLLTNDMNMPYERIFVAELGS
                     DGWELPEELSISDDQATNIGTVCISADGKWLFFTACGLKNGFGSCDIYYARRKDNRWI
                     EPRNAGAVINTSAWEAQPSVSSDNRFLYFASNRSGGQGGMDIWCCEMTEMKDGYFFFS
                     KPQNLGRKVNTPANDFSPFIHADGKTLYYSSEGKYGFGGSDIYVSRYLDGGWSEAENL
                     GYPINSRFNDDGLAVSPSAKTAFFASNREGSIGGSKDLFRLHLPEIFKPNVTGYIKGF
                     VYDAESGERLEADIEITLIETGSTHKVTSSLTEGFITTVNAGEMYAMHVRKDGYLFYS
                     RHFDLKQAAEFHQAEQFDINLEPVKQGGIFVLNNVFFDFNSDVLKPESEFELKKLTNF
                     LRDNIGISIEISGHTDNTGSVDYNVNLSERRAKAIVNYLSAFIDPERLLYRGYGSSRP
                     IADNETEAGRAKNRRCEIKILSK"
     gene            complement(12443..>12923)
                     /locus_tag="PKJ43_00065"
     CDS             complement(12443..>12923)
                     /locus_tag="PKJ43_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ70977.1"
                     /translation="IHPKDPAVDVALKFHSILFGSPYISYFSYTLPEKLKETLSFWIK
                     YWKTNSTYLLESDFEAYDPVHRYTILKAGNEKKQIIAFYARVESFDLQSFNFEMADLI
                     NSSDYPYLAVRGTPKGKVDYIIHDYSGKYLSRGALKFKRGVAVIEVPIGGFARLIVK"
BASE COUNT         4103 a         2612 c         2430 g         3778 t
ORIGIN      
        1 acttaaaaat cttcttgtta tttatttgac tcaattggaa taagctcaaa atcaaaaaga
       61 ttcagcacaa aaaaacctgt acaacaaatg ctgcacaggt taatgtttat atagtataaa
      121 cttagttacc gctattcatc gaaataataa attcttcatt cgtggtagtt ttcaacaatc
      181 tgtctcgcaa gaattccatc gcttcaagag aattcatgtc tgcaaggtaa ttacgcaaga
      241 tccaaatttt atcgagcatt gccttgtgaa taagtaagtc atcacgtcgg gtactcgaaa
      301 gcgtaacatc aactgcaggg aagatacgtt tgttcgacaa tttgcgatcg agctgcaatt
      361 ccatgttccc ggtaccctta aattcctcga atataacatc atccatcttc gagccagttt
      421 cggtaagggc cgtggccaaa atagtgagtg aaccaccttc ttcaatgtta cgggctgcac
      481 caaagaacct tttgggtttg tgcaatgcat tggcatctac ccctcccgac aaaacttttc
      541 cagatgccgg ttgcacggta ttataagcac gggcaagcct tgtgattgaa tcgaggagaa
      601 taaccacatc atggccacat tcggtgagac gttttgcctt ctcgagaacg atgtttgcca
      661 cgcgtacatg gcgttctgcc ggttcatcaa atgtagaaga aacaacttct gcattaacgc
      721 ttctggccat atcggtaact tcttctggac gttcatcaat aagtaaaaca atcatataaa
      781 cctcaggatg attagcggca attgcattgg ctatttcctt aagtaatact gttttacccg
      841 tttttggctg agccacaatc aatccacgtt ggcctttccc aatcggggca aaaaggtcta
      901 caatacgaac agacaatgaa gaatcaccct ttcctgtaag attgaatttt tcgttcggaa
      961 aaagtggagt taagtggtcg aatggaaccc tgtcgcgaac atattctggc gctctgccat
     1021 taatttctag cactttaata agaggaaaat atttttcgcc ttctttcggc ggacgaatag
     1081 ttccttttac tgtatcgcca gttttcagac caaagagctt gatttgcgac tgtgacacat
     1141 aaatatcgtc gggcgaatta aggtaattgt aatcagaaga acgaaggaat ccatacccat
     1201 cttgcataat ttcgagtaca cccgaattgg tgataaatcc ctcaaagtca aagattgtct
     1261 tatcttgctg cttggcattt ctgtaattct gacgtgcatt aggattcacg tttccaggat
     1321 caacttgttg ccgttcaggt cgtttttcat aattacgatc agtcggtaaa gacacctcta
     1381 ctttcttttc tacaggagct gcaattggtg ctttttcggc aacaacaaaa tcatccttat
     1441 ccgtaaggtc ttcaggttca atttctggca aaatcatatc gggtttgcct acaacatagt
     1501 tttgtttggg cttgtctgaa ggatatggct ggctagaatg atttttatct acaaactttt
     1561 cttttgaatt atcctgattc ctgttccgtt gttcacgatc gttctgtggc tttttactga
     1621 agaatttttt atcagagttt tcgttttgct ggcggggttt gaaaaaagga atttcttttt
     1681 gcacctgacg gggtgaattt tcttctttct gctcaatgat tggctcttct cgctgttcgc
     1741 tgggctgttc aattttttct tcaggcttaa cttgctgatt atctttagaa gtaatttgaa
     1801 ctttttcttc cgaagtacgt aaaatacgaa tcctttttct tttgaaaatt gaatcttgtg
     1861 attctccctt tttagggctg tctgtaattt ccgaatgttg ttttgaaggt tgggcaggct
     1921 tttcttgttt ttgttttacc tcagaggcaa gtattgcctg attgtcgaga attttatata
     1981 caagttcctg ttttttaagt ttttccggct tcttgatctg gagatttttg gctatctcgc
     2041 gaagatcatt aaccaacatt tgatttagtt caataatatc gtacatatct tgttaaatta
     2101 taagctattg aaaagctata ctataaatat ttctttgttt gattatgtat ttggaatgta
     2161 gtcgtgcgtg tagactatta atcagaaaaa tccattgcaa ttatacggca atatgaatta
     2221 ataagcaaca aagattaatt tttttttctt ttcgggctat tttcttcata tactaagagc
     2281 caatcttttg tatttcagtt ttatctacta tagatcgact agttgcgaat catttatttt
     2341 aatgaatttt tgatgctttt tgtgctactt ttaagtaaac ttagaagaaa atattaaaag
     2401 cattaaaact cattatccaa gatatcggcc actgtaatat cgcagaaaga ttaaatctca
     2461 acaaatctaa atgaacatga atagtataaa aattgttaca gaggacaatt tatttacatt
     2521 tgcatacaaa atttaaaaat aatgaatata gctgaaaaca aaatggtaac cctgagctat
     2581 gtactccggg ccgacagtgt gaatggagaa ataattgagc aaacaaccaa cgattctcca
     2641 ctgagatttg tgtttggtct tggccaaatg ctacctgctt tcgaatcaaa cctttctgga
     2701 ctcaagcaag gcgatgattt tgaaatgacc ctcagtgcac gcgaagctta cggcgagctc
     2761 gacgaaagtg ccataatcga tttagataaa gaagtttttt ttatcgacgg acaatttgat
     2821 gaagaaagat tcatgccagg cactcaggtg cctatgcaaa ccaatacagg tcaacgcctt
     2881 aacggtatag taattgaagt aaccgaaaca tcggtaaaga tggactttaa ccatcctttg
     2941 gctggcaccg acttacattt taccggaaaa gttatcgaag ttcgcgacgc tactgaagaa
     3001 gagcttactc caatggggtg cggttgtgga tgtggctctg attgtggctc tgatcacgac
     3061 tgttcttcag aatcgggtca ctgcggatcg ggaggttgtg gttgctaaaa aatttttaaa
     3121 aatatgaatt catttgtaaa catttttcgg ctgacaacat ttggcgaatc ccacggttct
     3181 ggcatcggag gtattattga tggctgccct gccgggttgg ttctcgattt agaaaaaata
     3241 caatatgaac tggaccggcg aaaacccggt caatcgaaaa ttactaccca aagaaacgaa
     3301 accgatacgg tggagtttct ttcggggatt ttcgaaggta ctaccacagg aacccctatt
     3361 ggcttttttg taaaaaacaa agaccatcat tcgtctgatt atgattcatt aaaagatatt
     3421 tttcgtccat cacatgctga ttacacgtat actcaaaaat acggattacg cgatcatcgg
     3481 ggaggcggtc gttcttcagc ccgtgaaacc attgcccgag tagtgggtgg tgccatcgcc
     3541 aagcaactac ttatgcttga aaatattagc atacaagctt ttgtacatag cgttggagaa
     3601 atcaccctcg ataaatccta tttcgaactc gatcttacaa aaaccgaaga taatattgta
     3661 cgctgcccgg atgtaaaaac cgctgaagcc atgattgccc gtattgaaga agccgggaga
     3721 aaccacgata ctgttggggg aacaatacaa tgtgtaataa aaggggtacc agcaggtttg
     3781 ggagcccccg tttttaacaa actacacgcc gatctggggt ttgctatgct tggtatcaat
     3841 gcagtaaaag gatttgagta tggatcaggc tttgatggca cgaaactctc aggatcggaa
     3901 cataacgaca tttttgtgac cgaaggagga aaagtaaaaa caataaccaa tcactctgga
     3961 ggaattcagg gtggaatctc taacggacag gatatctatt ttaatgtagc atttaaacct
     4021 attgcaacct tattgaaaga acaaaaaaca atagataaca aactggaaga aaccacaata
     4081 aaccccaaag gacgtcacga tgcatgtgtg cttccaagag cagttcccat tgtagaaagc
     4141 atggccgcac ttgtacttgc agaccacctg ctaatgagca aactttcgaa attataatat
     4201 aaaaataagt tgtaatataa ttttttagta ttttagaagc caaagttgga aatatgaggc
     4261 aactaaaaat aaccaaacaa gtcacaaacc gagaaaccct ttcattggat aagtatctcc
     4321 atgaaatagg gaaagtagaa ttgttgacac cagataaaga agtagaacta gccaagagaa
     4381 ttaaaaaagg cgacagagaa gcattcgaga agctgatcaa agccaacttg cgttttgttg
     4441 tatctgttgc caaacaatat caaaatcaag ggttaggcct tcctgatctt atcaatgaag
     4501 gaaatttagg gctgattaaa gccgcacaac gcttcgacga aactcggggt ttcaaattca
     4561 tatcctatgc agtgtggtgg ataagacaat cgatactgca atcgcttgcc gagcaagccc
     4621 gtattgtgcg tttgcctctc aataaaattg gttctatcaa caagataaat aaaacgttta
     4681 accaactgga acaagaattc caaagggaac ctacggctaa agaaatttca gaaatacttg
     4741 accttacacc cgacgttatt gaagagtcga tgcgggcttc gggacatcac ctgtcgatgg
     4801 acgcaccaat ttacgaagaa gaagccagca atatgtatga cgtgcttctg aacatggaca
     4861 cacccagccc cgacaaaggc ttattgaaca gctctctaca acttgagatt gaaagatccc
     4921 ttgcaacatt gggtgaacgt gagtctgaaa tcttacgata ctattttgga ttaaatggca
     4981 cccgaccaca cacccttgaa gaaattggag aggtatttgg cctaactcgt gaaagagttc
     5041 gccaaataaa agaaaaagcc attaaaaagc taaaaaacaa ttatcggaat aaattactga
     5101 aaagttacct aggtgaataa aagttgaaat cccgccccgt tgcgggattt tttatcgtac
     5161 atctatttca aaaacaagta attttgtaag tgaaatagca aagaaataga aaacataaaa
     5221 taccatgact cgattcatag ctccatcgat actctcggcc aattttctcg atctgagaaa
     5281 agacatagaa atgataaacc aaagtaatgc tcattggatt cattatgatg ttatggacgg
     5341 agtctttgtc ccgaacattt catttggatt accaattcta aaacaaatct catcaattgc
     5401 acaaaaaccc ctcgatgtgc acctgatgat agccaatccc gaccaataca ttactgcatt
     5461 taaggatgca ggggctgcaa atattacagt acacatagaa acttgccccc atttaaatag
     5521 gactataaat ttcatcaaag aacaaggact gatggcaagc gtatgcctca accctcatac
     5581 tcctattgag atgctcgaag aaatcattca ggacattgat atggtgctta ttatgtctgt
     5641 aaatccaggc tttggtgggc aactttttat agaaagcact taccggaaaa tagggaaact
     5701 caagaaaatg attctcgaca aaaatttaaa aacactcatt gaagttgatg gcggggttaa
     5761 tttcgaaaca ggaaaaaaac ttttcgaggc aggtgccaat gttttagtat caggaagttt
     5821 catttttcat tccgacaatc caaccaaaag aattgatgat ttgttatctg tttaaaattg
     5881 ataacgatgg aagacaacaa tgatatctac gacaacataa aagatgtaat caatagtata
     5941 ccggacaatt acaaaatact tgaagagacc atcaacattg atactcagaa agaatattac
     6001 gaatcgtcaa aagggattag catcgaccca gcgctcgata ttccggaaga gatgatcaac
     6061 caactcaatg aaggaaagtt ttcaagtacc gaagattgta agattttgct acagaagctg
     6121 gcactccttg attcagtaga agcatatcgt acgatagaaa aattttgtct tgtagctcct
     6181 cctgaaatta aagagtgggc cattcttgcc aaacaacaaa gccggatggt aatgcaatgt
     6241 tccctgcttg acgaacagca ggttttcatt tcgaccggac taggtgggaa aaacgacaaa
     6301 ctaaggtatt tactcatctt ccccttttca aaaaaacagg tgagcaaatt ccaactcgac
     6361 gccttagaaa aagaattgct ttattttctc cctaaaaacg aaggagaggt agaagaattt
     6421 gagattcaaa aaaagtatgc aacctcactt atccttcttc cattgaaagc tccggtcaac
     6481 gaaataattc aggagatact ggcagagtgc aaccagtttg gcaatttttt aagtcataat
     6541 gcaatgatta ctaacatgaa aaaattctct catgaagaaa tctctgcatt tattgaagaa
     6601 gaagaagaaa agtaagagaa aagaaatgca tttttttagc cacgataagg gctttttttt
     6661 tggtagtgta aacctattaa tttatatttg caccgctttt agcaagggtc ctgtagctta
     6721 atggatagag catctgacta cggatcagaa ggttgggggt tcgagtctct ccaggatcac
     6781 aaaaagcaaa gagttacatc ataaaaaatg taactctttt tttattagtt aactctcgta
     6841 attgagctta atgtggatat ttcttgggga caagctcacc cgaaagcatc agttgagcgt
     6901 tcatagccat cgaagccaat tcatcttccc ctttgtaaac aacaaccgtt gatataaact
     6961 ttattttttc agttactgaa cgagcgatta aagagctata agcaattcct cctgttaaca
     7021 aaatagcatc aacttctcct tcgagaacaa ccgccatttc gcctatagct tttgctacct
     7081 gatatacaaa agcttcaaca tagaaacggc attcttcatc tccatcttct gcaaaatcaa
     7141 caatttcttt catactgtta gtattcaggt aagcaaccaa accgccttct cctgttacca
     7201 ttcttctaac ttcatcttca ataaaatttc cacaaaagca agcttcaatc aactgaccag
     7261 caggcaaagt gccactacgc tcaggactaa aaggcccctc gccatcaagg gcgttattta
     7321 catcaacaac acgccccttc ctgtgagcac ctacagatac accacctccc atgtgggcaa
     7381 ctatcaaatt gatttcttcg tattttttcc caatcgattg agcatataag cgaccaactg
     7441 ccttatggtt aagtgcatga aaaattgatc tccgctcgaa acgaggatgc cccgatacgc
     7501 gtgcaacagg ttcaagttcg tcggtaacta caggatcggc aatataggca tgagcacccg
     7561 ggataatctt cgcaatagca tgggctaaca aggggcctaa attggaagca tgttgcccca
     7621 ttactccttt tcgcacatgt gtgagcatcc gatcatcaac ttcatatacc cccgattcga
     7681 gaggataggt caggcctcct ctccccataa cgatactgat atcgttgagt gaaataccgg
     7741 caatttcaag atttccaaca atcaaatcgt accgaaattc aagctgagaa agaattgtcg
     7801 gatagaggac caactcatct aggtaatgtt ttatggtttg ctcgaagagg catgtttcgt
     7861 tctgatatac agcaattttt gtcgatgtag aacctggatt gattaccaat actttaggcg
     7921 cactcataag attcggtatt aacacactac agcagctaaa gctatagagt ttagctttgt
     7981 ttcaacacta tcgcccctcg atgaaagaac cacaggaaca gatgctccaa ccaacatggc
     8041 agcaattgac gcattaaaca gtttagtatt cgctttataa aaaacattgc ctgattcgat
     8101 attcggaaaa agcaagcaat cggcatttcc ggcaactggc gagttaaaat tctttatttc
     8161 agcagcttct ttgtctaatg caagatccaa ccccattggt ccatcgcaaa ccgaccccgg
     8221 aaaatcacca tttagccaac gctgcttcaa tagcacggca tcggttgttg ctggcatctt
     8281 tgatataacc tgctcggtgg cagcaacaaa agcgaccaat ggtttttcta ttccaatccg
     8341 gtgtgcagtt gaaataaggt attgaactat gatggctttt tgttctaccg ttggcaaggg
     8401 aattattgca acatcactaa ctattaacaa tttatcataa ccaggggttt gcattaccgt
     8461 aatatgcgtt agtaaattac ctttaggcag caagccaacc gttttatcaa gcaaagcttt
     8521 cataaatcta tcggtactaa taagcccttt cattagtatg tcagctttcc ctttagacgc
     8581 aagttctacg gccattctcg ccgcgtcatc ttcatcgggg caatcgataa tttcaaatat
     8641 atgggattca aatcctgcca acaaacattg ctcttcaata atcttcctat taccggttaa
     8701 tatccctttt atccaaccac gccgaacggc ctctccaaca gcttcaatag aatgactatc
     8761 gaccccattg gctacaacca tacttttcac tggtttagac tttaatccgt taaaaatatc
     8821 ttcgagttta cctatttttt ccatattaat cctatcactt ttattctagc aaccagtatc
     8881 cttttttttg caaaaattac ttcttttcat aagataaaac aaccagcatt tgtcagtttt
     8941 ttaagcttag taaaacactt aagtactgat taaataaaca aaatatcata ttcccggttc
     9001 aacgttgaaa agacataaat gaacaatcaa ttatataaac agtctcttta agagggataa
     9061 aactaaaaaa acgggatatt tgtagcactg aagcgatact tttaatggaa gaatatgttg
     9121 acatacatac gcatcatgta aacattgagt gggagcaact tgccattcac aactatgatt
     9181 tgcctctcaa cgagattccc tcagtccagt acttttcggc aggttggcat ccttggcatc
     9241 taaatggttt ttcagcagat gaatgcaaaa gtgagtttaa aagactagct tcttacaaga
     9301 atttcgttgc tgtaggagaa tgcggtctcg ataggaagaa ggggaataac tgggatgaac
     9361 agatcctggc tttcgagaca cagcttgaaa ttgcaaaaga gtataaaaaa ccattgatac
     9421 tccacgcagt aaaatcgtat tctgaccttc ttcaactatt aaaaatgcat caattcgaga
     9481 acaaagtatt gatacatgga tttattggga atgagcaagt catcatgcaa ttcagcacct
     9541 tcgacaccta tttttctttt ggaaaaagca ttttcaacga tcattcaaaa tcaattgaat
     9601 tgctaaaaac aattgctcct gaaaaaatat ttttcgaaac cgatgatagt aatttttcaa
     9661 tcagcgatat atatcttcgt gccgcgacaa ttcggcaggt ttcggttgaa gaattgagca
     9721 ggattacaaa aaataatttc agaaaattca ttggtgatga actggttaga tagaacagaa
     9781 cttcttatag gcccccaaaa tattgagaaa ctgaaatcgg ctcatatact ggttgttggc
     9841 cttggagggg tgggtgctta tgcagccgaa atgctttgcc gggccggaat tggccaatta
     9901 accatagttg atggcgataa tgtagaactc acaaatatca acagacaact tccagcatta
     9961 cacagtaatt tggaaaaacg caaaacttca gtcatggcag aacggctaaa agacataaat
    10021 ccggatattg aactaacaat tatcgatgaa tatattcgcg acgaacgaat gattgagata
    10081 cttgacggca actttgatta tgttgtcgat gcgatcgaca cgttagcccc aaaaacattt
    10141 cttatttacc attcagttca aaaagggcta agaatagtga gttcaatggg ttcaggagga
    10201 aaatttaacc ccgaactggt taaatcgagc gacatttcga aatcatataa ctgcaatctc
    10261 gcccggatgc ttaggaaacg tctctcaaaa ttaggaatca agaaaggggt taaagttgtg
    10321 ttctcacccg aggaagtaga tgaaaaagct ataagattgg aagagggtac caataaaaaa
    10381 tctactgttg gcacaatctc gtatatgccc ccgttgttcg gctgccatat cgcatcggtt
    10441 gtgattcgcg accttgtcac tttggaacaa ccatcagaat aggaatctta ttttgaaaga
    10501 attttaatct cgcaacgacg gtttttagcc cggccagctt cagtttcgtt atctgcaatc
    10561 ggacgcgaag acccgtatcc acggtataaa agccgttcag gatcaatgaa tgccgacaaa
    10621 tagttaacaa tagcttttgc cctgcgttca gataagttaa cattatagtc tacactcccg
    10681 gtattatcgg tatgccctga tatttcgatt gatataccaa tattatcacg aaggaaattt
    10741 gtaagttttt ttagctcaaa ttctgattcg ggcttcaaaa catctgagtt aaaatcgaaa
    10801 aaaacattat taagcacaaa aatgcctccc tgcttaaccg gctcaagatt tatgtcgaac
    10861 tgttcggctt gatgaaattc tgctgcctgt tttaaatcaa aatgccggga ataaaaaaga
    10921 tatccatctt tgcgtacgtg catggcatac atctcccccg cattaaccgt tgtaataaac
    10981 ccctctgtca acgaagaagt aaccttgtga gtacttcctg tttcaattaa ggtaatttca
    11041 atgtcagcct caagcctttc ccccgattct gcatcataga caaagccttt tatatatccg
    11101 gtaacattcg gtttaaatat ttcgggtaaa tgaagtcgga aaaggtcttt cgatcctcca
    11161 attgagcctt ctctgtttga tgcaaaaaaa gccgtctttg ccgaaggaga aaccgccaac
    11221 ccatcatcgt taaatcttga attaatggga tatccaaggt tttcagcttc gctccatcca
    11281 ccatccaaat accgtgatac atagatgtcg ctcccaccga acccgtattt tccttccgaa
    11341 gaataataca acgttttccc atcagcatgt ataaagggcg aaaaatcatt tgccggtgtg
    11401 tttacttttc taccaagatt ttgcggtttc gaaaagaaaa aatacccatc tttcatttct
    11461 gtcatttcgc aacaccaaat gtccatgccc ccttgcccac ccgaccggtt tgatgcaaag
    11521 tacaaaaatc ggttgtctga agaaactgaa ggctgagctt cccatgccga agtattaata
    11581 actgccccgg catttcgggg ctcaatccaa cgattatctt tccgccgagc gtaatagata
    11641 tcgcaacttc caaatccatt ttttaaccca caagccgtaa aaaaaagcca cttaccatcg
    11701 gccgaaatgc atactgtacc aatatttgtt gcttgatcat cagaaattga taattcttca
    11761 ggaagttccc atccatcaga gcccaattct gcaacaaaaa tcctttcgta aggcatattc
    11821 atgtcattgg ttagtaaacg cgtaaaatat aatgctgagt cgttagtcga aattccaggc
    11881 caatattctt gccggctggt gttaataatt tctccgggtt tatcaataag tacctgccgg
    11941 ttttcggcta tcgattgaat agaaaaacgt gccgagttaa tcctgttatt aacaaaagaa
    12001 gaatctccag ccttcgcata aggttgataa aataagtaaa aattaagagc ttctttgtaa
    12061 ttgccttctg taaaatatag cccacccaac aacttataag cagccttata atataaagaa
    12121 tcgatactaa gtgccttatg aagagcaaat ttttgctgtt ctgttttttt taattcttcg
    12181 cttatatcgg aaagaagcaa ataaggtgta atagctgttg tgtcagactg gattgctttt
    12241 aaaatcagat tttctgcttc taagtaatct cctgatagaa ttgcgacttt tgcttgttca
    12301 accaaactaa tcgattttct ggttcctgtt tttgctgttt tgcacgaaga aaacatgaat
    12361 ataaaagcca ccaaagtcac taggaaaatg cacaaataag ggaaaaatgg aactagcctg
    12421 tttcttctca tgaagaagat cattatttaa caatcaaacg ggcaaaaccg ccaataggaa
    12481 cttcaatcac agccacacca cgtttgaatt taagtgcacc acgacttaag tactttcctg
    12541 aataatcgtg gattatgtag tcaacctttc ctttgggtgt tccacgaaca gctaggtagg
    12601 gataatcgct agagttaatt aagtctgcca tttcaaaatt aaaagattga agatcgaaag
    12661 attcaacccg ggcataaaag gcaataattt gctttttttc gttccctgct tttaaaatgg
    12721 tataccgatg aacaggatcg tatgcttcaa aatcactttc aaggagatac gttgaatttg
    12781 ttttccaata tttaatccaa aaactaagtg tctctttaag tttttcaggt aaagtatatg
    12841 aaaaatatga gatgtaaggc gacccaaaaa gtatggagtg gaattttagt gctacatcaa
    12901 cagctgggtc tttaggatga ata
//
LOCUS       DAOPUW010000002        10438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_6430_length_10438_cov_3.749109, whole genome shotgun sequence.
ACCESSION   DAOPUW010000002 DAOPUW010000000
VERSION     DAOPUW010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 10438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10438
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6430_length_10438_cov_3.749109"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PKJ43_00070"
     CDS             complement(<1..421)
                     /locus_tag="PKJ43_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ70978.1"
                     /translation="MNINRENYEIFYVDYLDGTLSVEEVDLLLGFLKQNPDLKAQLEG
                     IEQMVFEPEKISAPLFNHLKKNDLDKVEVFEETCIRSIENQLSTIENEQFELFLSGNP
                     SAKQEYKLFQATIFQPDLTLVFPKKHELKKTASRRIPF"
     gene            complement(431..916)
                     /locus_tag="PKJ43_00075"
     CDS             complement(431..916)
                     /locus_tag="PKJ43_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNZ70979.1"
                     /translation="MTTAEYNQIVEQYANGLYRYILKNIRDTERASDLVQDSFEKLWM
                     RSEDIQFSKAKSYLFSIAHNTMIDSVRREKRQVVTDVADMPVIADRSQYSDLKEVLDE
                     GLKKLSEIQRSVVLLRDYEGYSYEEIGQITNLSESQVKVYIFRARLFLKNYIGQIEHV
                     I"
     gene            complement(1124..1561)
                     /locus_tag="PKJ43_00080"
     CDS             complement(1124..1561)
                     /locus_tag="PKJ43_00080"
                     /inference="COORDINATES: protein motif:HMM:NF025262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ70980.1"
                     /translation="MDIFVAKLNSDTTADQIKELFGQYGKVSSTKLIMDRETGMSKCY
                     GFVEMEDESEGAAAIEHLNDTNFMGNYIVVKQSEPRGNEKRSFDRKPSFGGGGGGRRP
                     DGNRGRFDRNGGGGGGYNRGGDRDNRGGGGRDFRRGGDYNRYE"
     gene            complement(1809..3563)
                     /locus_tag="PKJ43_00085"
     CDS             complement(1809..3563)
                     /locus_tag="PKJ43_00085"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNZ70981.1"
                     /translation="MVIDIRTLILVLFIILSIQALVLFFTSVIVKGQRGIKCWGIGIL
                     LLALGFVCMFLRTYKEIEVITIILGNLSHFAGFIFLYVGSQHFFNHEKKIMPHVVVSL
                     VFLIAITYYTIIEDQVNTRAILYSFLASALLLVNGVFLLKERHPEVRKSVAFVSIIFL
                     MGSAFLFVRFFYLLFFNPIHNFFNGDFFQMPSFIGAMLIGLMLTFGFILVINQRLNAQ
                     VTTTAQHFKLIFNTIPDIATVTEARSGKFVYVNDEFFHVTGHSRKEIEGRSVFDVSLW
                     KNPNDRQKLINQLKEKSPMITQEFEFLRKDKNELTGLVSANPIVIGDKECVLFIIRDI
                     TDLKKKESDLAQKTLELEKSNAEKDTFFSILAHDLRGPFSSILNLSEVLSDQSFQISD
                     AEKEKMIISLHRNAISTNELLNNLLNWAAVRRKRFSYQPVESTFFEAVGETLPTLYDA
                     AVNKQISLVNNIPDETKVYADRYMFQTIIRNLVLNAIKFTYPGGRVELALQIENDDTQ
                     QFIVSDNGIGMSTMMAESIFKSDQKTSRQGTLGEPSSGLGLILCKEFVELHQGQIRVE
                     SEEGKGTRFIFSLSKKQV"
     gene            complement(3699..4763)
                     /gene="aroB"
                     /locus_tag="PKJ43_00090"
     CDS             complement(3699..4763)
                     /gene="aroB"
                     /locus_tag="PKJ43_00090"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNZ70982.1"
                     /translation="MSIPTFVNDIRVTNALEQELGSIVAHYPAGKVYIVVDENTKKHC
                     LPQLLSCEGLASVPVMEIASGEENKSLESVVRVWNFLEENNADRKSLLINLGGGMLSD
                     LCGFAASCFKRGLHFVNIPTTLLSQVDASVGGKTGINFNGLKNEIGVFSQPQTVIIAS
                     RFLQSLDRDNLLSGYAEMLKHGLIYSRKHWDDCLAFDLQNIDYERLNQVIAESVAIKE
                     YFVVKDPTEKNIRKALNLGHTAGHAIESLALHQNRPILHGYAVAYGLIVELFLSSMKV
                     GFPMEKVVTITNWINETYGKFQVQESDFEALYLKMTKDKKNEAGRINFTLIPEIGNVE
                     INIDCDKAQIMDALRFFAES"
     gene            4830..5315
                     /locus_tag="PKJ43_00095"
     CDS             4830..5315
                     /locus_tag="PKJ43_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ70983.1"
                     /translation="MDKPADNKFIFQLNNQSRSAGFFVLYIFCFFALLAASCVFIFTN
                     LNVLLFISIVVTLLFITIFHLYKPSYFELLVSETSFKINYYSVATALKNYHTVEIEHG
                     SFKGFEVRKRYAGLQKQLILTIGSKLGLADYPPISISILSKNELSQVITVLTKIKIQE
                     I"
     gene            5327..6445
                     /gene="dprA"
                     /locus_tag="PKJ43_00100"
     CDS             5327..6445
                     /gene="dprA"
                     /locus_tag="PKJ43_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNZ70984.1"
                     /translation="MPSTLQYKIALSLFPRIGSINARKIVSLLGGVEAVFEMAESELR
                     KMPDIGKSLIRTLIDERENVLSKADEEINFIEKYGIKTFFYLDKDYPRRLALCEDSPV
                     ILFQKGTATLNQQKIISIVGTRNATDYGRQQTEELISGLASLGINVMIVSGLAFGIDI
                     TAHKAALKNNFQTIGVVGHGLDKIYPSMHANIAREMIASGGGVLSDFVSGSKIDPGNF
                     PRRNRIVAGLADCTIVIESGEKGGALVTADIANSYNRDVFAYPGRTNDPFSKGCNNLI
                     KQNKAALIESASDLIQFMGWEPNTQPQQQVLFVDLNNEEQQIVDLLKRSETITTDIAA
                     IDLKMNVQTINALMLSLEFKGVVKSLPGNRFKLISRCY"
     gene            6492..7187
                     /locus_tag="PKJ43_00105"
     CDS             6492..7187
                     /locus_tag="PKJ43_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ70985.1"
                     /translation="MKRISITILITVLYLSTMAQLKVPGVNWKESYSFDKSNKFKVDF
                     YDKKGNLMRAMDYQTYYQTQGKDFDVLLVDDGRGNNTETIFDLENEVAIQIFKSKNVP
                     YMHNAGRFIFPDSASTKMLELVPTSETKDILGYACKKYTYTYKKIFGECWICPEISLS
                     NDYGIFRAAKMAALHNTLSVGGFVMEMTTEDKTGAKTVMRTVSLNNSESYSANLKNAD
                     MGTAINKVSYYTF"
     gene            complement(7227..7619)
                     /locus_tag="PKJ43_00110"
     CDS             complement(7227..7619)
                     /locus_tag="PKJ43_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HNZ70986.1"
                     /translation="MMDYYLLKTLKNTTIDEAEKVVTEKLKESGFGVVTYLDVSKTLN
                     QKIGAEFRPYRILGACHPHFAHKALLANDKIGVMLPCNVCLQVIDNKDVEVFTINPLV
                     AMQAMNEPGVQELANEVYQKLEGLLEKL"
     gene            7828..9036
                     /locus_tag="PKJ43_00115"
     CDS             7828..9036
                     /locus_tag="PKJ43_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNZ70987.1"
                     /translation="MKVILIAILITLFSTTGALSQKPTAWRGGTNGNYPDKGLLKAWP
                     QNGPDILWVAEGLGDGHSSPVFANGKIFVSTMIDTTGFIIILDLKGKELKRYPYGTEF
                     FESYPGARSTPVIVGDWLYIYSGLGTIYAFDAEKGTLRWQRNMTKEADGENIKWGVTE
                     TLVVDGDKIYCTPGGKKFNIVALNRLDGTFVWNSPGVGDLSAYCTPQLIDLGNKKILV
                     TMMANHIVGVDASTGKTLWSHEQPNQWSVHANTPIYANEMIFCTSGYGQGTVALSLSP
                     DGNSVSKAWFNPKFDSRMGGAVYIDGYLYSSGDKNREWRCLDAKTGAEKWASVEIGKG
                     DIISADGLLFLYSEKGELALAEANPNAFKLLSQAKVEKGSAQHWAHPVINDGTLYVRH
                     GNALIAYKIK"
     gene            9095..10342
                     /locus_tag="PKJ43_00120"
     CDS             9095..10342
                     /locus_tag="PKJ43_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HNZ70988.1"
                     /translation="MKKFIAIFFILFSVNAWSQVPQFRGPNRDGNYPDKGLLKQWPEN
                     GPKLLVETSGIGNGYSSASFSNNKIYITGMIDTLDYLSCIDLSGKILWKTAYGSSWNQ
                     SFPESRSTPTIMNGKAYVSSGRGVVACIDAESGKIIWSVDAFKKEEGAYGIWGVAESI
                     LLVDGKAIFTTGGNNTTMVALDANTGKEVWKSPSLRDNLAYVSPILIEKNGKKLIIGV
                     TASYIFGVNPESGNIAWSFDYKKIAPPENDNSRGLINCTSPIYHDGILYITSGYNHIG
                     AEFKLKDDLSGVDFLWKDGVLDNHHGGVVLVDGYVYGSNWVNNNKGNWCCINFETGEK
                     KYEETFKNKGSIIFADGMLYIYSEQPGFVGLVKPSPEKFELVSSFQITKGNGPHWAHP
                     SIYDGKLFIRHGDYLLVYDIKEK"
BASE COUNT         3263 a         2144 c         2001 g         3030 t
ORIGIN      
        1 agaaaggaat tcttctggat gctgttttct ttagttcatg ctttttcgga aaaacaagcg
       61 tcaaatcggg ctggaaaata gttgcctgga aaagtttata ttcctgcttt gccgaaggat
      121 ttcctgataa aaataattca aactgctcgt tttcaatcgt tgaaagttgg ttctcaatag
      181 atcggatgca cgtttcctca aaaacttcta ctttgtctaa gtcgtttttc ttcaggtggt
      241 taaataaagg tgcgctaatc ttttccggtt caaaaaccat ctgttcaata ccttcaagct
      301 gagcctttaa atcggggttt tgtttgagaa aaccgagcag aaggtcaact tcttcaacag
      361 aaagagttcc atcgaggtag tcgacataaa aaatctcgta attttctctg tttatattca
      421 tagcaatcta ttaaataaca tgttcaattt gcccgatgta gtttttcagg aaaagccttg
      481 ccctgaaaat atacacttta acctgtgact cgcttaagtt ggtaatctgc ccgatttctt
      541 cgtaagagta cccttcataa tcgcggagca acaccaccga acgttgaatt tcgctgagtt
      601 ttttcagccc ttcgtctaac acctctttca agtcggaata ttgcgatctg tcagcaatta
      661 ccggcatatc ggccacgtcg gtaaccactt gtcgtttttc tctccgtacc gaatcgatca
      721 tcgtattgtg agcaatactg aaaaggtacg acttggcttt tgaaaactgg atatcttcac
      781 ttctcatcca aagtttttca aagctatcct gtaccaaatc agatgcacgc tcggtatccc
      841 taatattctt aagaatatag cgatagagtc cattggcata ttgctctact atttgattgt
      901 actcggcagt tgtcatctgt atctttcaaa catagtatat gacgttgcag gcaggcaaat
      961 agttacagat tgttggcttt ttttctggaa aaattatttg tctttgatta tcagaacatt
     1021 gtgcaagtat aaagtataat gaatcgtctg ataggctttt gtagaaaggg agaaatacgc
     1081 agaaaaaaaa agcaggattt tcctgctttt tttaaaagat cgtttattcg tatcggttgt
     1141 aatctcctcc tcttctgaaa tcacggcctc caccgccgcg attgtcgcga tctccgccac
     1201 ggttgtagcc tccgccaccg ccaccgttac ggtcaaaacg gccacggttt ccatcaggtc
     1261 tgcgtccacc gccgccacct ccaaatgaag gttttctgtc gaatgaacgt ttttcattac
     1321 cacggggttc cgattgtttt acaacaatgt agtttcccat gaagttggtg tcgtttaggt
     1381 gctcaatagc agcagcacct tcagattcat cttccatttc aacaaaacca tagcatttcg
     1441 acatgccggt ttcacggtcc ataattaact tggtagaact cactttacca tattgaccga
     1501 ataattcttt gatctggtca gcagttgtgt ctgagttcag ctttgcaaca aaaatatcca
     1561 taaaaaaaaa taaaaaatta atgaaattga ataattaact cgttagaaga tgtgtaggtt
     1621 aacaaaacga aaaaagattg tgttgcaaat tctatacaaa tcctaataag gcaaatctat
     1681 tgattcttag caataagtaa aaggattttg agaaaattat cttaaaatga tgaaattcac
     1741 cggtagaacg ctgttttttt gccgaataaa ccttcgaaaa atttgaaaat tagtctggtt
     1801 actgttcgtt acacctgttt ttttgacaac gaaaaaatga atctggtccc ttttccttct
     1861 tcactttcta ctcgaatttg tccttgatgc agttcaacga attctttaca aagaatcaaa
     1921 cccaggcccg aacttggttc tcccagcgtt ccctgacggc tggtcttctg gtctgattta
     1981 aatatcgatt cagccatcat agtgctcatt cctatgccat tatcggatac aataaactgt
     2041 tgcgtatcat cattttcgat ttgcaaagcc aattcaaccc ggccaccagg ataggtaaat
     2101 ttaattgcgt tcaaaacaag gttacgaata attgtttgaa acatataccg atcggcatat
     2161 accttagttt cgtcgggaat gttatttacg agggaaattt gcttgtttac agccgcatcg
     2221 taaagagtag gcaaggtttc accaacggct tcgaaaaaag tagattcaac cggttggtaa
     2281 ctaaatcttt tccgcctgac agctgcccaa ttcagcaaat tattcaacaa ttcattggtt
     2341 gatatggcat tgcggtgcag cgaaataatc attttttctt tttctgcgtc tgaaatttgg
     2401 aacgattggt cggataatac ttctgaaaga tttaatattg aactaaatgg gcctcgaaga
     2461 tcgtgtgcta gaatagaaaa aaaggtgtct ttttcagcat tcgatttttc gagttcaaga
     2521 gtcttttgcg ccaggtcact ttcctttttc ttcaaatcgg taatgtcgcg aataataaac
     2581 agaacacatt ctttgtctcc aataactatt ggatttgcag aaacaagacc agtaagttca
     2641 tttttatctt ttcgaagaaa ctcaaattcc tgagtaatca tcggcgattt ttctttcagc
     2701 tgattgataa gcttttgcct gtcgttcggg ttcttccaaa gacttacatc aaaaaccgac
     2761 cttccttcta tttccttcct cgaatgaccg gtaacatgga aaaattcatc gttgacatat
     2821 acaaactttc ccgacctagc ttcggtcact gtggcaatat ctgggatcgt attaaatatc
     2881 agcttaaaat gttgggcagt agtggttacc tgagcattta agcgctgatt aataaccaaa
     2941 ataaaaccaa acgtgagcat taagcctatc agcattgcac caatgaatga tggcatttga
     3001 aagaaatcgc cattaaagaa attgtgtatc gggttgaaaa aaagcaaata aaaaaaccga
     3061 acaaataaaa aagcacttcc cattaaaaaa ataatagaaa caaaagctac tgatttcctc
     3121 acctcagggt gcctttcttt aagaagaaaa accccattga ccagcaataa ggctgatgct
     3181 aaaaaagagt ataagatagc tctggtatta acttggtctt ctataattgt atagtatgta
     3241 atggcaatca gaaaaactaa agacaccaca acatgtggca taattttctt ttcgtggttg
     3301 aagaaatgct gcgaaccaac atagagaaaa ataaacccgg caaaatgaga taagtttccc
     3361 aagataatgg taataacttc aatttcttta tatgttctga gaaacataca aacaaatccc
     3421 aacgcgagaa gcaaaatacc aataccccaa cacttgattc ccctctgccc ttttacgata
     3481 accgatgtga aaaacaaaac taaggcctga atactcagaa taataaataa cacaagaatt
     3541 aacgttctga tatcgattac cataccctaa attttgacac cataatccac actccgtttg
     3601 aaaagtcaaa agatatgatt ttttgatttg acatacaatg tattatcaat gcattttttg
     3661 gtaaaatgaa aaaataaatg gctcacagca atactttttt acgattcggc aaaaaaccgt
     3721 aatgcatcca ttatttgagc tttgtcacaa tcaatattta tttcaacgtt acctatttct
     3781 gggattaatg taaaattaat gcgtccggct tcgttctttt tgtcttttgt catttttaga
     3841 tataacgctt caaaatcact ttcctgaact tgaaatttcc catacgtttc attaatccag
     3901 ttagttatcg taaccacttt ctccatagga aaaccaactt tcattgacga aagaaaaagc
     3961 tccacaatta atccataagc cactgcatat ccgtgaagga tcggtctgtt ctggtgcagg
     4021 gcaaggctct caatggcatg tcctgcagta tgcccaaggt tcagggcctt gcggatattt
     4081 ttctctgtgg gatctttcac cacaaaatat tctttaattg ccacagattc agcgatcact
     4141 tggtttaagc gctcataatc tatattttgc aagtcgaaag ctaaacaatc gtcccaatgt
     4201 ttacggctat atatcagccc gtgcttaagc atttcggcat accccgaaag taaattatcc
     4261 cgatcgagcg actgcaaaaa acgtgaagca ataataactg tttgtggctg actgaaaaca
     4321 ccaatttcgt tcttcagccc attaaaattg atgccggttt taccacccac agaggcatcg
     4381 acctgagaaa gaagggttgt aggaatattg acaaaatgta aaccccgttt aaaacaagaa
     4441 gcagcaaaac cacataggtc gctgagcatc cctccaccca ggttgatcaa caacgattta
     4501 cggtcggcat tgttttcttc aagaaaattc caaacacgca ccaccgattc gaggcttttg
     4561 ttctcctctc cgctggcaat ttccataacc ggtaccgaag ccagcccttc gcatgaaagt
     4621 agctgtggaa gacaatgttt cttcgtgttt tcgtcgacaa caatatatac tttaccagcg
     4681 gggtaatgcg ctacaatcga accaagttcc tgctctagcg cattggtaac cctaatgtcg
     4741 tttacaaatg ttggaatgct cattttttac acttattttt cagctccaaa attataataa
     4801 tttgttactt tgcagaaaga cttttaatta tggacaaacc agccgacaat aaattcattt
     4861 ttcagctaaa caaccagagc cgctctgcag gcttttttgt tttatacatt ttttgctttt
     4921 ttgctctatt agctgcaagt tgcgtcttca tatttacaaa cctgaatgta cttttattta
     4981 tttctattgt tgttaccttg ttgtttatca caatatttca tctttataaa ccatcgtatt
     5041 tcgaattact ggttagtgaa acatcgttca agataaacta ctattctgtg gctaccgcct
     5101 taaaaaatta tcataccgta gaaatagaac acggtagctt taaaggtttt gaagtcagaa
     5161 aacgttatgc tggcttgcaa aaacagctca ttttaaccat cggatcgaaa ttggggctgg
     5221 ccgattaccc gcccataagc atctcgatac tcagtaaaaa cgaactttca caagtaatta
     5281 ccgttttgac taaaataaaa atccaagaaa tataatccct ttttacatgc cctcaacgct
     5341 acaatataaa attgcccttt cgctttttcc acggatagga tcgatcaatg cccgaaaaat
     5401 agtgagcttg cttggaggcg tcgaggccgt atttgaaatg gctgaaagtg agcttcgaaa
     5461 aatgcccgac atcgggaaaa gccttataag aacactcatt gacgagcgcg aaaatgtctt
     5521 aagcaaagcg gatgaagaaa taaacttcat tgaaaaatat ggaatcaaaa cattcttcta
     5581 tctcgataag gactatccac gccgcttagc attatgcgaa gattcgcccg ttattctctt
     5641 tcaaaaagga acggcaaccc tcaaccagca aaaaattatt agcattgttg gcactcgcaa
     5701 tgccaccgat tatggacgcc agcaaaccga agaactgatc tcgggattgg cctcactggg
     5761 aattaacgta atgatagtaa gcggattggc atttggaatt gatataaccg ctcacaaggc
     5821 cgcgctgaaa aacaatttcc aaacaattgg ggtcgttggg catgggctcg ataaaatata
     5881 cccttcgatg catgccaaca tagcccgcga gatgatagct tcaggcggag gggttctttc
     5941 tgattttgtt tccggctcaa agatcgatcc gggcaatttt ccccgccgaa accgcattgt
     6001 tgctggcctt gctgattgta ccattgtaat cgaatcggga gaaaaagggg gcgcactggt
     6061 tactgccgat attgcgaact cgtataaccg cgatgtattt gcttaccccg ggcgaaccaa
     6121 cgatccgttt tcgaaaggct gtaacaatct cataaaacag aataaagcgg cactcatcga
     6181 atcggcttca gacctgattc aattcatggg atgggaaccc aatacccaac ctcaacagca
     6241 agtgcttttt gtagacttga acaacgaaga acaacaaatt gtcgacctac tgaaaagatc
     6301 ggaaacaata actaccgaca ttgcagccat agatttaaaa atgaacgttc aaacaattaa
     6361 tgcactgatg ctaagcctcg agtttaaagg ggtggttaag tcgcttcccg ggaacaggtt
     6421 taaattaata tcgaggtgtt attgattttg ttttttcgta atttgttgta ataattttaa
     6481 actaaactac catgaaacga attagtatta caatcctgat tactgtcttg tatttgagca
     6541 caatggctca attgaaagtt cccggggtaa attggaaaga atcttactcg ttcgacaaat
     6601 ccaacaaatt taaagttgat ttctacgaca aaaaaggcaa ccttatgcga gccatggact
     6661 atcagacgta ttaccaaact caaggtaaag atttcgatgt acttcttgtt gatgatggcc
     6721 ggggaaacaa caccgaaacc attttcgacc ttgaaaatga agtggccata caaatattta
     6781 aatcgaaaaa tgttccctac atgcacaacg ccggtaggtt tatttttccc gatagcgcat
     6841 cgacaaaaat gcttgaactg gtaccaacaa gcgaaacaaa ggatattttg ggctatgcct
     6901 gcaaaaaata tacctatacg tataaaaaaa tctttggaga atgctggata tgcccagaga
     6961 ttagcctttc aaacgattat ggtattttca gggccgcaaa aatggcagca ctgcacaata
     7021 ctttatccgt aggaggtttt gtaatggaaa tgacaaccga agacaaaacc ggagctaaaa
     7081 cagtaatgcg cacagtatca ttaaacaaca gtgaaagtta ttctgccaac cttaaaaacg
     7141 ccgacatggg aacggctatt aacaaggtta gttattatac tttttaacct cgaactggtc
     7201 ataacattgt tgggacaaaa ccggggttac agtttttcga gtagtccttc aagtttttgg
     7261 taaacttcat tagcaagttc ctgcactccg ggttcattca tggcttgcat tgcaaccaac
     7321 gggttgattg taaacacctc aacatcttta ttatctataa cttgcaaaca tacattacaa
     7381 ggtagcataa ccccaatctt gtcgttggca agcaatgctt tgtgtgcaaa atgtggatgg
     7441 caagccccta gaattcgata tggcctgaat tcagctccaa ttttctgatt caatgtttta
     7501 ctaacatcga gataagtaac cactccaaaa cctgactctt taagtttttc agtaacaact
     7561 ttttccgctt cgtcaatcgt tgtatttttt aaagttttca ataaataata atccatcatt
     7621 tcaaaaattt attagttgat acaaaacaac aaaacaagcc taagtatcaa cgtgacaaaa
     7681 attacattaa gggcaaaatg cctcataatt gtcaggagtt gtatactgct gataatccct
     7741 gttattggtt tgttaaaata ccgtaacagc tatttgataa ggaatatagt ttactaattt
     7801 cgcaaggaac ttaaaaataa tacatcgatg aaggttatac taattgcaat tctaattaca
     7861 ctattttcaa caacaggtgc tttgagtcaa aaaccaacag cctggcgagg tggaaccaat
     7921 ggtaattatc ctgataaagg gcttttaaaa gcatggcctc aaaatggacc agacatcctc
     7981 tgggttgcag aagggttagg cgatggacat tcatcacccg tttttgccaa tggaaaaatt
     8041 ttcgtttcaa ccatgatcga tacaaccggg ttcatcataa ttctcgattt aaaaggcaaa
     8101 gaattgaagc gctacccata cggaaccgaa tttttcgaaa gttatccggg cgcgcgttca
     8161 actccggtaa ttgttggaga ttggctctac atatatagcg gacttggaac tatttacgct
     8221 tttgacgctg aaaaagggac gctacgctgg caaagaaata tgaccaaaga agcagacgga
     8281 gaaaatatta agtggggagt taccgaaacc ctggtcgtag atggagataa aatatattgt
     8341 acgcccggtg gtaaaaaatt caatatcgtt gccctcaacc ggttggatgg tactttcgta
     8401 tggaattcgc ccggagttgg cgacttgtcg gcctactgta ccccacagct gatagatctc
     8461 ggcaacaaaa aaatactcgt aacaatgatg gcaaaccata ttgttggtgt tgacgcatca
     8521 accggaaaaa cactttggag ccacgaacaa ccaaaccaat ggagcgttca tgccaatacg
     8581 ccgatctatg ccaatgaaat gattttctgt acaagtggtt acggacaagg aactgttgcc
     8641 cttagccttt cacccgatgg aaattcggtt agtaaagcct ggttcaaccc aaaattcgac
     8701 agccgtatgg gcggagccgt atatatcgac ggctatcttt attcttcagg cgataaaaac
     8761 cgtgaatggc ggtgcctcga tgccaaaacc ggagcagaaa aatgggcgtc agttgaaatt
     8821 ggtaaaggcg atatcatctc tgccgacggt ctccttttct tatatagcga aaaaggagaa
     8881 ctcgctcttg cagaggctaa tccaaatgca ttcaaacttt tatcgcaggc aaaggttgaa
     8941 aaaggaagtg ctcagcactg ggcccatcct gtaattaacg atggaacgct ttatgtgaga
     9001 cacgggaatg ccttaattgc ttataaaata aaatagtata caaaccagat tgtcccgggg
     9061 atcacactcc gggatgattt atttaataag attcatgaaa aaatttatcg ctattttctt
     9121 tattcttttt tctgttaatg catggagcca ggttccgcaa ttcaggggtc caaaccgtga
     9181 cggaaattat cccgacaagg ggcttttaaa acaatggccg gagaacggac caaaattact
     9241 cgtggaaacc agtggaattg gaaacggcta ttcttcagct tctttttcca acaataaaat
     9301 ttacattacc ggaatgatcg acacgcttga ctatttatcg tgtatcgact tatcgggaaa
     9361 aatcctttgg aaaacagctt atggctcctc ctggaaccag tcgtttcctg aaagccgttc
     9421 cacaccaacc ataatgaacg gcaaggccta tgtaagttcc ggacggggag tggttgcctg
     9481 tatcgatgct gaatctggga aaattatctg gtcggttgat gcatttaaaa aagaggaagg
     9541 agcctacgga atttggggag ttgccgaatc aattttgttg gttgacggta aagctatttt
     9601 caccaccgga ggaaacaata ccaccatggt tgccctcgat gccaataccg gtaaagaggt
     9661 ttggaaatca ccttcgcttc gcgataattt ggcctatgtt tcacccatat tgattgaaaa
     9721 aaacgggaaa aaactgatta ttggcgtaac agccagttac atctttggag taaatcctga
     9781 aagtgggaat attgcctgga gttttgatta caagaaaata gcccctcctg aaaatgataa
     9841 ttccagaggg ttgatcaact gcacatcacc gatttatcac gatggcattt tatatatcac
     9901 cagtgggtac aaccatattg gtgctgaatt caaactgaaa gacgaccttt cgggtgttga
     9961 ctttctctgg aaagacggag tcctcgacaa ccatcacggc ggtgtagtgc tggtcgatgg
    10021 ttacgtttat ggatcgaact gggtcaataa caataaagga aactggtgct gtatcaactt
    10081 cgaaactggc gaaaagaagt acgaagagac tttcaaaaac aaaggaagta tcatttttgc
    10141 cgacggaatg ctttacattt attccgaaca acctggtttt gtcggactgg taaaaccctc
    10201 tcccgaaaaa tttgaattgg ttagcagttt ccagatcacc aaaggcaacg ggccacactg
    10261 ggcacatccg tcaatttacg atggaaaact tttcataagg catggagatt atttgcttgt
    10321 ttacgacatt aaagagaaat aaaaagaaaa tattacccga agggttcaat gaacagaaaa
    10381 ccaatacaaa gaggaacgat catgcttgcc gtagtaatgg cgggactttt ggcttggt
//
LOCUS       DAOPUW010000003         9347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_7405_length_9347_cov_3.573289, whole genome shotgun sequence.
ACCESSION   DAOPUW010000003 DAOPUW010000000
VERSION     DAOPUW010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 9347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9347
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7405_length_9347_cov_3.573289"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            459..887
                     /locus_tag="PKJ43_00125"
     CDS             459..887
                     /locus_tag="PKJ43_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ70989.1"
                     /translation="MAQINIEILVDANELIQSNKTGQIGKEHVIMTDDQIDDVYLDGD
                     VSHLVTRVEQGDVINWTIKTRTGVLVYFEDIVINSKKKWFSEEGGKPVIDPSKTKITG
                     TISKKIKSGDICSYNLIFSMGPELNGIWNFDPFIEARIRP"
     gene            932..2716
                     /locus_tag="PKJ43_00130"
     CDS             932..2716
                     /locus_tag="PKJ43_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HNZ70990.1"
                     /translation="MVICYCVPSINANTIKLSEKQIDELNIEYERALLQAISITNGQK
                     SLAYSLEALRIAIVLEDDLKIAQSYNEISLDYEKTGDLSNALQYVIMAAKKYEEIGKS
                     EGIGMAYNRIGTIYHLQGNRDMAILYMNKALSVHKQMGAYNSYADDLNNLGEIYRLNN
                     QYRLALQNFSEANAFFQQSNDSINLGYVSGNIGLVYAAIDQMDSANFYLSVATKILNR
                     KGDFYPIAVYLTETANIYLKQGNTNAALEKAKEALALSKRENYKEQIRDANKLLSDIY
                     ALRSDYEKAFGALNDYYNAKDSISNSKIISDMAEIRADYEVSKKETEIKSLQAINTLR
                     NRISFGLGVGFLLVTLLTIILVRMNKKVRITNSLLTIQKAEVEQKNEIIRTALIEKET
                     LLKEIHHRVKNNLQIISSLLNLQSQNVTDKKAIEAFSESQRRLHSIALVHQKLYQNEN
                     LARINIKEYFEDLVETIYQSFYSKVKSISYRLNIADVNLDVDTAVPLGLVINELVTNA
                     FKYAFNKVNEGAISITLNKEEGHSYSLIVKDNGCGLPENLTMNETESLGLKLVNILVR
                     QLEGTIDVKSNNGAKFTIHFVDVIQSKF"
     gene            2723..3463
                     /locus_tag="PKJ43_00135"
     CDS             2723..3463
                     /locus_tag="PKJ43_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ70991.1"
                     /translation="MDQLKILIVEDELIIAEDIKRQLLEIGYDVIGIASSYSGALKII
                     ETKIPDLAIVDIVIKGPKSGIDLGRHLHDELNIPFIYLSSHSDKNTIKQAKDSHPNAY
                     LLKPFRPESLFASIEIAICNASLNEKPQDKEVKIESLVVKDCLFIKKGHLFYKIKVSE
                     ILFVKGEGNYLEIYLQNGKKHLIRSTINNFIQNLSKYAFFQCHKSYIINLECIDIVSY
                     SFVKIKEHEIPLSKSKKEELLMAMKTFS"
     gene            complement(3616..4449)
                     /gene="pyrF"
                     /locus_tag="PKJ43_00140"
     CDS             complement(3616..4449)
                     /gene="pyrF"
                     /locus_tag="PKJ43_00140"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNZ70992.1"
                     /translation="MNATQLFEEIKRKRSFLCIGLDTDIQKIPSHLADTSDPVFAFNK
                     EIIDHTHDLAVAYKPNLAFYESLGSRGLVSLEKTVHYLRENHPEIFIIADAKRGDIGN
                     TSDLYARAFFNHFDFDAVTVAPYMGEDSVKPFLGYEGKWVILLALTSNKGAFDFQFLK
                     DEKSGDVLFESVLKTSKAWGTTDNLMYVVGATKAEKLEEIRTIVPDHFLLVPGVGAQG
                     GSLQEVAKYGMNNQCGLLVNSSRAIIYASNGEDFAAKARQEAENVQLEMEELLLEAEL
                     L"
     gene            4560..5576
                     /locus_tag="PKJ43_00145"
     CDS             4560..5576
                     /locus_tag="PKJ43_00145"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNZ70993.1"
                     /translation="MKYTIKIHSILAIALSLFWPLVAQPANPVIDSLIHLITYDSYVA
                     HFDSLRTNNGHFRKVIEAEVQSADHDACRDYIFRSMQRVLGAENVSLHKFETGNYKGL
                     ANVIGIKKGSSPDSGLIIISAHYDSNNSNETQLTNCAPGANDNGTGLAALLEIARVLS
                     EIETECSVIFAAWDFEEQMMYGFPVGSNHWFSHFVRNSKKKKADEVFPIKRKEIIANI
                     NFDMIGNPMETLDGKLILWACLGNRSHKKFIDSYITIFNEYTQGISAINGGMIPYSDH
                     YTFAARRIPAVENLESGFSDDPYYHTCNDNIENEKNINFSFATEVTRGGLAYILSQCL
                     LKND"
     gene            complement(5618..7798)
                     /gene="recQ"
                     /locus_tag="PKJ43_00150"
     CDS             complement(5618..7798)
                     /gene="recQ"
                     /locus_tag="PKJ43_00150"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HNZ70994.1"
                     /translation="MIDSQYLTGQLHKYFGFDRFKGQQEEVIKSLMEGNDAFVLMPTG
                     GGKSLCYQLPALLLEGTAIVISPLIALMKNQVDSIRSFGTEDGIAYFLNSSLSKSAIQ
                     EVKEAVLKGKTKLLYVAPESLTKEDNIEFLKQIKVSFYAVDEAHCISEWGHDFRPEYR
                     RIRPIIQEIGVAPVIALTATATAKVQHDIQKNLGMLDAKVFRSSFNRPNLYYEIRPKH
                     KTTAEIIKFISNNQGKSGIIYCLSRKKVEQLAENLQVNGIKALPYHAGMDAATRSKHQ
                     DMFLMEEVDVIVATIAFGMGIDKPDVRYVIHYDIPKSLEGYYQETGRAGRDGGEGVCI
                     TYYSYKDIQKLEKFMQGKPVSEQEIGKQLLLDTVSYAESSVCRRKILLHYFGEIYHQD
                     NCGACDNCLHPRERIEAGEELALALEAIVVVNQRYKMEHIANILVGISNAAVKSFKHD
                     ELDIFGLGKDHDNKFWHAIYRQAMIAGYINKEIENYGLLKLTDKGQAFLDNPGSFIMT
                     KNHDYDAAIEEAAAQGGSSSGDPELFALLKDLRKKIAKKLDLPPFVIFQDPSLVDMSI
                     QYPITLQELQNIQGVGQGKAMRYGKEFVELISQYVDENEIDRPQDIVVKSVAQKSKNK
                     VTIIQNIDRKVSLDIIAKSLGMGMEDLLSEIEAIVNSGTKINIDYYIDDVMDDDYQND
                     IYDYFREESKEGSIEEAVKDLGENDYSVEDIRMVRIKFISEMGN"
     gene            8053..8901
                     /gene="tatC"
                     /locus_tag="PKJ43_00155"
     CDS             8053..8901
                     /gene="tatC"
                     /locus_tag="PKJ43_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNZ70995.1"
                     /translation="MGEKKQHNETEMSFLDHLEVLRWHLIRSVLVSFFFMVVAFISRN
                     FIFNEIVLKPKNPEFITNRLFKQMGEWLQLHFGLNAESIAINTQPLNIVNIDMAGQFN
                     SHLKISLVVGLIVASPYVIWELWRFIKPALHKNEERYAKGAVFFITSLFMLGVLFGYY
                     LIAPLSIHFLNTYIVSPEVENTIKLSSYISTINSVTFASGVIFELPIIVYFLSKVGLI
                     TQAFMRKYRRHAYVLLLIVAGVITPPDVFSQMLVFLPLLILYEVSVYISRSVERRKTI
                     TTEQER"
     gene            8939..>9347
                     /locus_tag="PKJ43_00160"
     CDS             8939..>9347
                     /locus_tag="PKJ43_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ70996.1"
                     /translation="MKLSTENIEKRYRNRTVVKQVSIEVNQGEIVGLLGPNGAGKTTS
                     FYMIVGLIRPNNGKIFLDQEEITHMPVYRRAQKGIGYLAQEASVFRHMSVEDNIRSIL
                     EMTDMTKHEQKEKLELLLSEFGLNKIRKSTGIQL"
BASE COUNT         2917 a         1858 c         1762 g         2810 t
ORIGIN      
        1 cgtcaatttc tttatatagc tcaggcccct ctatgctgat gttaactatt gctgagtcct
       61 taatttcatt cttatacctg aaggtaaaat cgggcagggc ttcttcccct ttttgctggc
      121 tcttaaagat tagtggcttt ataaagtact gcataccctc ttctcccaca aagaaggcac
      181 tgtaaaacct ggctgaacca cctgcacttt gatgccactc cgtttttttg agtgggttct
      241 ggtctccact tagtatttta ggatcaaata catcgtcttc agaaattaaa tgtattatcg
      301 ataaaggatt atttaaataa tttgtgatat gaggcgtatt atttgattta aaatgaaata
      361 ttgttgagat attctgtttt tgattgtaat aattcttatc tttaagtaat taaatgtttt
      421 gtttttccct attgtcaaac cctcttaata attgtattat ggcacagata aacattgaaa
      481 tcctggtaga tgccaacgaa ttgattcaaa gcaacaaaac tggccagatc ggcaaagagc
      541 atgtgataat gaccgacgat caaattgatg atgtgtactt ggatggggat gtaagccacc
      601 tcgttactcg tgttgaacaa ggcgacgtga taaactggac gattaagact agaactggtg
      661 ttttggttta ttttgaagac attgttatta acagtaagaa aaaatggttt tcagaagaag
      721 gtggcaaacc ggttatagat ccttctaaaa cgaaaattac cggaacaatt tctaaaaaaa
      781 ttaagtcagg agatatttgc agttataatc ttatttttag tatgggcccc gaattaaatg
      841 gaatatggaa ttttgatccc tttattgagg cacgaataag accgtaatga attttcgatg
      901 taaaaatctt cgaataggct taataatact tatggtcatt tgctattgtg ttccatctat
      961 caatgctaac acaataaaac tttccgaaaa gcaaattgac gaattgaaca tagaatatga
     1021 aagagctctt ttgcaagcaa tttcaattac caatgggcaa aaatctttag catattcttt
     1081 ggaagccctt cgtattgcca ttgttttaga agatgatctt aagattgccc aatcatataa
     1141 tgaaattagc cttgattatg agaaaactgg agatctttcc aatgcattgc aatatgtaat
     1201 catggctgca aaaaaatacg aagaaattgg taagagtgaa ggaattggaa tggcttataa
     1261 ccggattggc accatctatc atcttcaggg aaatcgtgac atggcaattt tatatatgaa
     1321 caaagcattg tcagttcaca agcaaatggg tgcctacaat tcttatgcag atgacctaaa
     1381 taaccttggt gaaatttata gactaaataa tcagtaccgg ctcgctttac aaaacttctc
     1441 tgaagccaat gcatttttcc aacagagtaa tgatagtatt aatcttggat acgtttcggg
     1501 caatatcggg ctagtctatg ccgctatcga ccaaatggat tcggcgaatt tctacctgtc
     1561 agttgcaact aaaatactta atcgaaaagg agatttttat cctattgcag tatatctcac
     1621 cgaaacggcc aacatttatc ttaaacaggg aaacaccaat gcagcattgg aaaaagcgaa
     1681 agaggcttta gctctatcca aaagagaaaa ctacaaggaa caaattcgcg atgcgaacaa
     1741 attgctatct gatatttatg cacttagaag cgattatgaa aaagcctttg gggcattaaa
     1801 tgattactac aacgcgaaag acagcataag taacagtaaa attatcagcg acatggctga
     1861 aattcgcgcc gattatgagg tgtcgaaaaa agagactgaa attaagagtt tacaggcaat
     1921 aaatacgctg cgaaacagga ttagttttgg acttggcgtt ggctttctgc ttgttacgct
     1981 gcttaccatc attcttgtac gaatgaacaa aaaagttaga ataaccaata gtttattgac
     2041 gattcaaaaa gctgaggttg agcaaaagaa cgaaatcatc cgaacagcac tgattgagaa
     2101 agaaacatta ttgaaagaga tccatcacag ggtaaaaaat aacctgcaaa tcatatcaag
     2161 cctacttaac ttacagtcgc aaaatgtaac ggataagaaa gcaattgaag ccttctcaga
     2221 aagtcagcgg cggttgcatt ctattgcatt ggttcaccaa aagctttatc aaaatgagaa
     2281 tttggcaagg atcaatatta aagagtattt tgaggacctg gtcgaaacga tctatcaatc
     2341 tttttattca aaagtaaaaa gtattagtta tcgtttaaat attgcagatg tcaaccttga
     2401 tgtagacaca gccgttcccc ttggactggt tattaatgaa ctagtgacaa atgcctttaa
     2461 gtatgctttt aataaggtaa atgaaggggc aatatccatt accctgaata aagaagaagg
     2521 acattcgtat tcgttaattg tgaaagataa tggttgtggt ctaccagaaa accttactat
     2581 gaatgaaaca gaatcgctgg ggttaaaatt ggtaaatatt cttgtaagac aacttgaagg
     2641 gactattgat gtgaaatcaa ataacggagc taagtttact attcatttcg ttgatgtaat
     2701 tcaatctaaa ttctaaatta ttatggatca acttaaaata ttaattgtag aagacgaatt
     2761 gatcatcgct gaagatatca aaaggcaatt gttggaaata gggtatgatg ttattggcat
     2821 tgcttcttct tatagcggag cgttaaaaat tattgaaacc aagattcccg accttgccat
     2881 tgtagatatc gtaataaaag gcccaaaatc gggcattgat ttaggccggc atcttcatga
     2941 tgaattaaat attccattta tttacctttc aagccattca gataaaaata caataaaaca
     3001 agccaaagat tcacatccga atgcctattt gttaaaacct tttcgaccgg aaagcctttt
     3061 tgccagtatt gaaatagcaa tttgcaatgc atcattaaac gaaaaacccc aggataaaga
     3121 ggtcaaaatc gagagtcttg ttgttaaaga ttgtctgttc attaaaaaag gtcacctttt
     3181 ctataaaata aaagtaagtg agatcctttt tgtcaagggt gaaggaaatt atttagagat
     3241 atatcttcaa aatgggaaaa aacatttaat ccgttcaacc ataaataatt ttattcaaaa
     3301 tttatcaaaa tacgcttttt ttcaatgcca taaatcgtac atcatcaatc tcgagtgtat
     3361 tgatattgta tcctattctt ttgtgaaaat taaagagcat gaaattccat tatcaaaaag
     3421 caaaaaagaa gaattattaa tggctatgaa aacgttctcg taagttcaaa aaaggatcac
     3481 taaaaccagg gtatgagcgt actataagtg ttgactttgt aaatacacaa aaaaaaggaa
     3541 caccccacaa gctgtccctc tttttatatt tagccttttt cctaaaggaa taaatcttct
     3601 ctcttatact aaaaactata ataactcggc ttccaaaagt aattcttcca tttcgagttg
     3661 aacattctca gcttcctgcc gtgcttttgc cgcaaaatct tccccgttag aagcatagat
     3721 aatagccctt gatgaattga ccaacaaccc gcactggttg ttcatcccgt actttgctac
     3781 ttcctgcaag cttcctccct gagcaccaac cccgggaacc aacaaaaaat ggtcgggaac
     3841 tatcgtacgg atctcttcca acttctcggc ttttgttgca cccaccacgt acatcagatt
     3901 gtcggtagta ccccatgctt tcgacgtttt cagaaccgac tcgaacaata catccccgct
     3961 tttctcatct ttcagaaact gaaaatcgaa agcccctttg ttagaggtta atgcaagtag
     4021 aattacccat ttcccttcat aacccagaaa tggttttacc gaatcttcgc ccatgtaagg
     4081 tgccacagtc actgcatcga agtcaaagtg gttgaaaaaa gcccgggcat aaagatcaga
     4141 agtattgcct atgtcgcccc gtttggcatc agcaataatg aatatttcag ggtgattttc
     4201 cctcagataa tgtaccgtct tttcgagact caccaaccct cttgagccca acgattcata
     4261 aaaagccaga ttaggtttgt aagcaactgc caaatcgtgg gtatgatcga ttatttcttt
     4321 gttgaaagca aaaacaggat cggacgtgtc ggcaaggtga ctgggtattt tctgtatatc
     4381 agtatctaat ccaatacata ggaaacttct tttccgtttt atttcttcga ataattgcgt
     4441 agcattcata aattgtaatc tatagtattt atgatgcaaa aatacgattt ttgaaggaag
     4501 cttaatttat gcgtgccatg taatgcagtt ttttattttc tttgagatga ttttcaagca
     4561 tgaaatatac aattaaaata catagtattc tggccattgc gctttctctg ttctggccat
     4621 tggttgctca acctgccaat ccggtaattg acagtcttat ccacctcatc acctacgatt
     4681 cgtatgtagc acatttcgac tcactaagga cgaacaatgg tcatttcaga aaagttatcg
     4741 aagccgaagt tcaatcggcc gaccatgatg cttgccgaga ctatattttc cgttcgatgc
     4801 aacgcgtact tggcgcagaa aatgtatcgc tacataagtt cgaaaccgga aactacaagg
     4861 gcctggccaa tgtaattggg ataaaaaaag gaagcagtcc agatagtggg ttaatcatta
     4921 tcagtgctca ttacgacagt aataattcaa acgaaacaca gcttaccaac tgtgcgccag
     4981 gcgccaacga caatggcaca ggcttggctg ccctgctcga aatagctcgg gtgttgtcgg
     5041 aaattgaaac ggaatgctcg gtaatttttg ctgcgtggga tttcgaggag caaatgatgt
     5101 atggttttcc tgttggttca aaccattggt tttcccactt tgtaagaaat tcgaaaaaga
     5161 aaaaagccga cgaggttttt cccatcaaac gaaaagaaat cattgcgaac atcaacttcg
     5221 acatgatagg gaaccccatg gaaaccctcg atggaaaact gatactttgg gcctgcctgg
     5281 gaaacaggtc acacaaaaaa ttcatcgata gttacattac gatattcaat gaatacaccc
     5341 aaggtataag tgcaattaat ggaggaatga ttccctactc cgatcattat acttttgctg
     5401 cacgccgaat acctgccgtt gaaaacctgg aatcaggatt tagcgatgac ccctactacc
     5461 acacctgcaa tgacaatatt gaaaatgaga agaatatcaa cttcagcttt gccacagagg
     5521 ttacccgcgg agggcttgcc tacattcttt cccaatgtct cttgaagaat gattagtcaa
     5581 gcgaaacacc ctgactaata aaaaataatt gcacgcgtta gtttcccatt tcagaaataa
     5641 acttgatacg aaccatccgg atatcctcta ctgaataatc gttctcaccc aaatctttca
     5701 ccgcttcttc aatggaacct tcttttgatt cttcacggaa gtaatcgtaa atatcgttct
     5761 gataatcatc gtccattaca tcatcaatat aatagtcgat atttattttg gtccctgaat
     5821 ttacaatagc ctcgatttca gaaagaagat cttccatacc catgccaaga gattttgcga
     5881 taatatcgag cgatactttc ctgtcgatat tctggataat ggttactttg tttttcgatt
     5941 tctgggcaac cgactttaca acaatatctt gagggcggtc aatttcattt tcatcgacat
     6001 actggctgat caactcaacg aattctttcc cgtaacgcat ggcttttcct tgcccaactc
     6061 cttgaatatt ttgaagttcc tgcaacgtta ttgggtactg aattgacatg tcgaccaacg
     6121 aaggatcctg gaaaataacg aacggtggca aatcaagttt ttttgcaatc tttttgcgca
     6181 gatctttcaa taatgcaaag agttcgggat caccggaact gcttccaccc tgtgcggctg
     6241 cttcctcaat agctgcatcg taatcgtggt ttttggtcat tataaaagat cccggattat
     6301 cgagaaaagc ttgtccttta tcggtaagct tcaacaatcc gtaattctct atttccttgt
     6361 taatataccc ggcaatcatt gcctgacggt aaatggcatg ccagaattta ttatcgtggt
     6421 ctttccctaa tccaaaaatg tcaagctcgt cgtgtttaaa cgattttacc gcggcattac
     6481 taatccccac cagaatattg gcaatatgtt ccatcttata cctctggtta acaaccacaa
     6541 tggcttcaag ggcaagagcc agttcttcac cagcttcaat tctttctcgt ggatgcaagc
     6601 aattatcgca agcgccacaa ttatcctgat ggtatatttc gccaaaatag tgtaataaaa
     6661 ttttcctccg gcaaaccgat gattcggcat aagaaacagt atcgagcagg agctgtttgc
     6721 caatttcctg ttcagaaacc ggtttaccct gcatgaattt ctcaagtttt tgaatgtcct
     6781 tatagctata gtaagtaata caaacacctt cgcccccgtc tcgaccggca cggccagttt
     6841 cctgatagta tccttcgagg cttttgggta tatcgtaatg gattacatac cttacatcgg
     6901 gcttatcgat tcccattccg aatgctatgg ttgccacaat aacatctacc tcttccataa
     6961 ggaacatatc ctgatgcttc gaacgggttg ccgcgtccat gccagcgtgg tatggcagcg
     7021 ctttaatacc gttaacctga aggttttcgg ctaactgttc cacctttttc cggctaaggc
     7081 aataaataat tcccgatttg ccttggttat tcgaaatgaa tttgatgatc tcggcagtgg
     7141 ttttgtgttt tggccttatt tcgtaataca ggttggggcg gttgaatgac gaacggaaca
     7201 ctttggcatc gagcatgccc aggtttttct gtatatcgtg ttgaactttg gcagtggcag
     7261 tagctgtgag ggcaataacc ggagcaactc caatctcctg aattattggc cgtatccggc
     7321 ggtattcagg cctgaaatcg tggccccact cagaaataca atgcgcttcg tctaccgcat
     7381 aaaacgaaac tttaatctgc ttaagaaatt caatattgtc ttctttcgta agcgattccg
     7441 gggcaacata caggagtttt gttttcccct ttaaaacggc ttcttttact tcctggattg
     7501 ctgattttga cagcgaagag tttagaaaat aggcgatacc gtcttcggtt ccgaagctgc
     7561 gaattgaatc gacctggttc ttcataaggg ctatcagtgg tgagataacg atcgcggtac
     7621 cctcgagcaa aagtgcaggc aattggtaac ataacgattt ccctcctccg gttggcatta
     7681 aaacaaatgc atcattcccc tccattaaac ttttgatcac ttcttcttgt tgtcctttga
     7741 agcgatcgaa cccaaaatat ttgtgtaatt gccctgttaa atattgacta tcaatcattt
     7801 tgaataatat taatctctac ccttttttcg atcggaacac taagatagca aattatatta
     7861 ctctaaaaaa tcgtttggca taattaaact tgtaaatgtt aattttgtat aaccaattaa
     7921 aacccaaagg caacgacaca aagaccaata atgatgggga tttggatgtt tttacagcta
     7981 cagatgattg cttaattgta tgttagattg atttttaatt taacgtatat ttgctgaccg
     8041 attaattttt ccatgggcga aaaaaaacaa cataacgaaa ccgaaatgtc gttcctcgat
     8101 catcttgagg tattgcgctg gcatttaatc cgttcagtcc tggtatcatt tttttttatg
     8161 gttgtagcct tcatcagccg aaattttatt tttaacgaaa ttgtccttaa acccaaaaat
     8221 ccagaattca ttaccaacag gctgtttaaa caaatgggtg aatggcttca gctccatttt
     8281 ggacttaacg cagaaagtat cgccatcaac acccaaccgt tgaatattgt taacattgac
     8341 atggccgggc aatttaattc gcaccttaaa atctcgctcg tagtagggct tattgtagca
     8401 agcccttatg ttatttggga gctatggcgt tttatcaaac ctgctttaca caaaaatgaa
     8461 gaacggtatg ccaagggtgc agtctttttc atcacctcgc tgtttatgct gggggtactc
     8521 ttcggatatt accttattgc gccactctcc attcattttc taaacactta tatcgtaagt
     8581 cccgaagttg aaaacacgat aaaactaagt tcatacattt cgacaataaa ctcggttact
     8641 tttgcatcgg gggttatttt cgagcttcct attattgtat acttcctgag caaggtgggt
     8701 ttaatcacgc aagcatttat gcgaaaatac cgcagacatg cttacgtttt gttgctcatt
     8761 gttgccgggg ttatcactcc acccgatgtt ttcagccaaa tgctggtgtt tttgcccctt
     8821 cttattttgt atgaagtaag tgtgtatatt tcacgatcgg ttgagcgacg aaaaacaata
     8881 accaccgaac aagaacgtta aactaacaat aaacgaatca ataccggaca gagaaaggat
     8941 gaaactaagt accgaaaata ttgagaaacg ataccgcaac cgcacggttg taaaacaggt
     9001 gtcgatagaa gttaaccagg gagaaattgt gggattgctt ggccccaacg gagccgggaa
     9061 aacaacttcg ttctacatga ttgttgggtt gattcggcca aacaatggga aaatatttct
     9121 cgatcaggaa gaaataaccc atatgccggt ataccgccgt gcacaaaaag gcattggtta
     9181 ccttgcacag gaagcttcgg tattcaggca catgagcgta gaagacaaca tccgttcgat
     9241 actggaaatg accgacatga ccaagcacga acaaaaagaa aaacttgaat tgctgctgtc
     9301 ggaatttggt cttaacaaaa tcagaaaaag tactggaata cagcttt
//
LOCUS       DAOPUW010000004         9341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_7411_length_9341_cov_3.451325, whole genome shotgun sequence.
ACCESSION   DAOPUW010000004 DAOPUW010000000
VERSION     DAOPUW010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 9341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9341
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7411_length_9341_cov_3.451325"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PKJ43_00165"
     CDS             <1..337
                     /locus_tag="PKJ43_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ70997.1"
                     /translation="PQLHTGNSMIATAPAVRLPKSVSTMKHLEDYLQTMDVLGARVVG
                     FNRLGEKFYPSIGLTPEQVYPYVVTLSDAVDGLSWVSFAELMENFRMLKDGHLLICIA
                     RLFNALQGK"
     gene            complement(403..891)
                     /locus_tag="PKJ43_00170"
     CDS             complement(403..891)
                     /locus_tag="PKJ43_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ70998.1"
                     /translation="MNNIDFTPEITHCSLADFSQITSEIKAFWGSDRTLAIHHPSLIH
                     EFGNTAFVIKKEDQVIAYLFGYYSQTESTAYIHLISAREGYQKMGLGQILYSHFMALA
                     KANNLTKIKAITRPFNTKSINFHTHKIGMKMLGEPNAEGINVIKNYAGYGEDRVVFEK
                     LI"
     gene            complement(960..1904)
                     /locus_tag="PKJ43_00175"
     CDS             complement(960..1904)
                     /locus_tag="PKJ43_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNZ70999.1"
                     /translation="MEHYNTRKNSSALSLAFWLNLVFSVVELVGGILTNSTAIATDAL
                     HDFTDAFAIGLAVWLEKTSQKKRTPKYSYGFKRFSMLSALGMSIFLVTGAILMIINAI
                     QSFFHQEEIHSVGMFWLAVAGILVNGFAFLKIKNEGKKTAHNHANPMQNQYQHQHVQK
                     TNYNNKAIMLHLLEDVLGWVAVLVGAAILHFTQWLWIDGVLAIAIALFIIFNASKNFI
                     DTMSVLLQSVPENVDIEELSGQLRQVEGVEDIHDLHVWSLDGSYTIGSLHVVIDETTK
                     THEKEIFDNVIRVMEKFQIDHPTIQIENKQDDCKLVDC"
     gene            complement(2055..2477)
                     /locus_tag="PKJ43_00180"
     CDS             complement(2055..2477)
                     /locus_tag="PKJ43_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNZ71000.1"
                     /translation="MRPKTEDKLLEKNTQPTSMRILVYDFLSNQQVALSLTEIENHLK
                     PADRITIYRTLKTFEEKGIVHSIQENNTTKYMLCNDLCDEKTHSDWHLHFYCKICRQT
                     TCHTDILLPDSIKGGFRIDEIRFFAKGICEKCLNETNM"
     gene            2635..3765
                     /locus_tag="PKJ43_00185"
     CDS             2635..3765
                     /locus_tag="PKJ43_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71001.1"
                     /translation="MKKFKHLIFLALALMWIGVGCEKESTPELTEKYLTVDDAKYKSG
                     KLPVGKENVITDVQMNKSVINGGSAILIVSASEPLDQIYISVDGIAGYYIYDVTSSGL
                     TYQILINLSQELDVETLVINVSAKTDEGAVSLIEDSGSIKVVEVGTGKLQVSLSWNKE
                     DDVDLHLFEPDGDEICYENPASFVGDETEFYFKFYVYLIEKYTSHDASSLKVDNEDDV
                     ELLLEYLAEIEDDIDTYEEFRSYNDNDFLGCLDLDSNADCEIDGIKNENITYSNPENG
                     TYTVAIDLYSKCTSGVEGAKYSVTAVYDGKALALSNPVGQFEDDNYGSGDSESQYVTI
                     GKFTISGSSKSALLETVDQRPVNDKLLLLKQKLLNAKAASRK"
     gene            complement(3923..5017)
                     /locus_tag="PKJ43_00190"
     CDS             complement(3923..5017)
                     /locus_tag="PKJ43_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HNZ71002.1"
                     /translation="MKNRFYHFILIMIVLHLSENTYSQNSMVQTNSTGIEIVTKLEKP
                     KQKFDEKDLAGYLLVYFKDQDQSAYMAISADGYTFTDLNGGNAIFIGSQLAEQKGVRD
                     PHITRGPDGAFYLAMTDLHIFGKRAGFRDTEFERPAEKYGWGNNRALVLMKSYDLIHW
                     THTNLRIDKAFPELGDIDCSWAPETVFDPKAGKMMVYFTIRYNNKECHMYWAYANDDF
                     TKLETTPKRITEIGGLDGDITKVGDQYQLFYVSGAKILHSVSDKINEGYPIDTPRIDP
                     EKVSTEAPNLFKRLGTNSYVLMYDVYGARPSNIGFSETTDFVTYKNLGHFNEGVMKTT
                     NFKSPKHGAVTYLTKKELQDIAKHWKVDLE"
     gene            complement(5124..6044)
                     /locus_tag="PKJ43_00195"
     CDS             complement(5124..6044)
                     /locus_tag="PKJ43_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ71003.1"
                     /translation="MKRNIFQKQDDSEIEETTENKVVSEINVDSEVENTTKSAPSGLT
                     DIINLVNINQVYDEHNPPNIVFKDFNFDIKDIKERGQFITIMGKSGCGKSTLLRYISG
                     LQVPTSGDVYIYGKKRTEKDRIPMVFQQYTSFEWKTVLQNVALPLILKGIDKDEANDK
                     AMEMIKIVGLAGHEKKWAKYPILSGGQLQRVAIARNLVVNPQILLMDEPFGALDTVTR
                     KQIQVFLRSIFENAKIDPTIVFVTHSESEAIFLSTDIIILDSGPASIRHHLKIELPEQ
                     RNDSVRYSTEFTDYVNKLGALMEDLKNERK"
     gene            complement(6047..6859)
                     /locus_tag="PKJ43_00200"
     CDS             complement(6047..6859)
                     /locus_tag="PKJ43_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71004.1"
                     /translation="MEIIKKLFTFAGEVDKKTALLITMLGWFLLIIVWWIISTLGWIS
                     STILPSPIDVILCYKTLLVERNLLYNTGYSIFINLGGYLQALALAIPLGFVVGLIPFF
                     RHLVSKQFEAIRFTPLPATTGIFMALFGIGLGVKVEFLAFGIFVYLLPAITQRIKDIE
                     TDDHLKALKQTMWTLNASPWMTLKHFYIPSVLSRFSMDVINLVAVSWTYIVIAEMVNA
                     QGGLGSMIYMATSRGSHVDQLYAILFLIIFIGFVQDKFLKLLDKKLFKFKYA"
     gene            complement(6889..8442)
                     /locus_tag="PKJ43_00205"
     CDS             complement(6889..8442)
                     /locus_tag="PKJ43_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HNZ71005.1"
                     /translation="MTTDYQNKTEKSIPGFPQKWTFGFQLFVLILIIGAVGAGVYFGY
                     PYLNKSSKGNKAELRAGFNNFVGFAPGIFMNGGAAPNKDSRMYTEFGLTFEAIRMDDM
                     QKLNDALKNGDLDFIFTTTDISPIGMDRSSDLAKMSVVQFLKIDDSRGADVFIVDKSI
                     NTVADLKGKKIACALGWPSNTLLHATLEAGGLTEQDVKILPMSDPFAAKTAFTTGNVD
                     ATVVWSPDDEECLKSRDAKILTSTELLPNIIMDGFIARKDVLEKKKDLFVKLSRAWLV
                     ANAEMRDPEKMAKAAQTYKIAFDVPDDVSIILDGMKKIHFANYGDNINFFGLSTDYTG
                     LTGQQLYTKMARVYKTGYGNKLNNIVPWAEASYPGIIQEINDLQGDAHKAEGQIVFET
                     PTAADEKAPAVAIKPIIINFATASWALTPEMKDKIENQLGQLSVEFAGMKVRIEGNTD
                     NVGSAEMNRDLSRKRAQSVADYLVKTYNFDPNRFIIVGNGPDKPVASNNSEEGKAANR
                     RTEFQLLGK"
     gene            complement(8482..>9341)
                     /locus_tag="PKJ43_00210"
     CDS             complement(8482..>9341)
                     /locus_tag="PKJ43_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71006.1"
                     /translation="PAIFKLFRRIVRSFHKAIIRWNPFDELEEQKQKMWETHDLFLKH
                     KSKIKQLRTDFEQMSNETRKVAESSKDEVQRLTKKASEVKQQMDKIIAEKGDSVKETD
                     EYVELQQQFINATSAGTRTNTILEKNIEWTTKYATRSNIFANLDRKLAIGATLLENKI
                     KDFEESINIMKKDWEMAAASRGATAILKEILGPGGQNWKLEYALEFVAGRISEDLAMT
                     AQNLEDLERNTIAFNFDSDEAYDKLLAIGNKLDAGQMEIPNPSRISNPNHKLTSEEKA
                     SAGPLGDIF"
BASE COUNT         2564 a         1773 c         1942 g         3062 t
ORIGIN      
        1 acctcaactt cataccggaa atagcatgat tgctactgca ccggcggtga ggctccccaa
       61 atcggtttcg acaatgaagc accttgaaga ttatctgcaa acgatggatg ttttgggggc
      121 aagggtggtt ggttttaaca ggctgggcga gaagttctac ccaagtatcg gcttaacccc
      181 cgaacaggta tatccgtatg ttgtaacact ttccgatgct gttgatgggc ttagctgggt
      241 gagttttgct gaattgatgg aaaacttcag aatgttgaaa gatgggcatt tgctaatttg
      301 tattgcccgg ctttttaatg cgttgcaagg gaagtagttg ttggtatcgc agcagtttta
      361 acttgctttg tttttcaatg ggttgagtag aatcgtttag gctcaaatta gtttttcgaa
      421 tacgacgcga tcttcgccat accctgcgta gtttttaata acattgattc cttctgcgtt
      481 aggttcgcca agcattttca ttccaatttt atgggtgtga aaatttatcg attttgtatt
      541 gaacgggcgg gtgatggctt ttatttttgt caaattgttg gcttttgcca aggccataaa
      601 atgggaataa aggatttgcc ctaagcccat tttctgataa ccttcccgcg cagaaatcag
      661 atggatataa gcagtacttt cggtttgaga gtagtagcca aataaatagg caatcacttg
      721 gtcttctttt ttaatcacaa atgctgtgtt cccgaattca tgaattagag aagggtggtg
      781 aatggccagg gttcggtcgc ttccccaaaa tgctttgatt tcggacgtaa tctgagaaaa
      841 gtcggccaaa ctgcaatggg taatttcagg agtaaaatcg atgttgttca ctttgttgat
      901 ggttgggttt gagtgcaaaa ttataaaaaa tagggttcct gaactaatgt tgtattcatt
      961 taacaatcga ctaatttaca atcatcttgc ttgttttcaa tttgaatggt tgggtggtcg
     1021 atttggaatt tttccatcac acgaatgacg ttgtcgaaaa tttctttttc gtgggtttta
     1081 gttgtttcgt caataacaac atgcaatgaa ccaattgtat aactgccatc cagactccaa
     1141 acgtgcaggt cgtgaatgtc ttcaactccc tctacctgcc gaagctggcc ggaaagctct
     1201 tcaatatcta cattttcggg aaccgattgc agcaggacac tcatggtgtc aataaagttt
     1261 ttcgatgcat tgaaaataat gaacagtgca attgctatgg ccagcactcc gtcaatccaa
     1321 agccactgtg tgaaatgcag aatggctgca ccaaccaaca cggctaccca gccaagcaca
     1381 tcttccagca aatggagcat gatggccttg ttattgtagt tcgttttttg cacatgttgg
     1441 tgttgatatt ggttctgcat tgggtttgca tggttgtgcg ccgttttttt tccttcgttt
     1501 ttaattttaa gaaaagcaaa tccgttgaca agtatcccgg caacagccag ccaaaacatt
     1561 ccaacgctat ggatttcctc ttgatggaag aatgattgta ttgcgttaat aatcattaat
     1621 atggcacctg ttacaagaaa tattgacatt cccaaagccg agagcattga aaaacgtttg
     1681 aatccgtacg aatatttagg cgtacgtttt ttttgcgagg ttttttcgag ccatacggcc
     1741 agaccaattg caaaagcatc ggtaaagtcg tgcagggcat cggtggcaat tgctgttgag
     1801 ttggtaagaa tacctccaac cagttcgacc acagagaata ccaaattgag ccaaaaggca
     1861 aggctgaggg cactgctgtt tttccgggta ttgtaatgtt ccattttctt tcaatgtttc
     1921 agtagatgat gcaaaaataa accgggaaaa tatgcaatgc tattgcaaac tttcatctgc
     1981 atagaacttt ttacctgctg tctatctttt ggatcaatct tccgtaattt tactgagatt
     2041 tttgccggat gaactcacat gttcgtttca tttaggcatt tttcgcagat ccctttcgca
     2101 aaaaaccgta tttcgtcgat tctgaaacca ccttttatcg agtctggcaa tagtatatcg
     2161 gtgtggcagg tagtttgtct gcatattttg caataaaagt ggaggtgcca gtcgctatgt
     2221 gtcttttcat cgcataaatc attgcaaagc atatactttg ttgtattgtt ttcctgaata
     2281 ctatggacta ttcctttttc ttcaaaggtt ttcaatgtgc ggtaaatagt aatcctatca
     2341 gcaggtttaa gatggttttc aatttctgta agcgataacg caacttgttg attcgaaaga
     2401 aaatcgtaca ccaaaatccg catacttgtt ggctgagtat ttttctctag taatttgtct
     2461 tctgttttag gtctcatttt catcgttcca tagtaatttc tgcaaggtat taaaaagaaa
     2521 acccatggtt gttactttgg tgctcagatt attgtagatt tacatatgat aaacatttgg
     2581 tttgcgaaag ttgcaaactt gaatgtcatc ttgataacaa ctaaaaacaa cagtatgaaa
     2641 aaatttaaac acctgatctt tttggcgctt gcactgatgt ggataggcgt tggatgtgag
     2701 aaagaaagca caccagaatt aactgagaag tacctcacgg ttgatgatgc aaagtacaaa
     2761 tcgggaaaat tgccagttgg gaaagaaaat gttattactg atgttcaaat gaataaatcg
     2821 gtaattaatg gaggttcagc tattttaatt gtttcggcat cagaacctct tgatcaaatt
     2881 tacatctcgg ttgacgggat tgccggctat tatatttacg atgtaaccag ttcgggcctt
     2941 acttatcaaa ttttaattaa cctgagtcag gagcttgatg ttgaaaccct tgtaattaac
     3001 gtttcggcta aaaccgacga gggggctgtt tccttaatcg aagactcggg cagcattaaa
     3061 gttgttgaag ttggaacggg aaaattacag gtgagcttgt cctggaataa ggaggatgat
     3121 gttgatctcc atttgtttga acccgatgga gatgagattt gctacgaaaa tcccgcttcg
     3181 tttgtaggag atgaaacgga gttttatttc aagttctatg tttatcttat tgaaaaatat
     3241 acttcgcacg atgcttcgtc tttgaaagtc gacaatgagg atgatgttga attattactt
     3301 gaatatcttg ctgaaattga agatgatatt gatacttatg aggagtttag gagctataat
     3361 gataacgatt tcttaggctg tctcgatcta gactcgaatg ctgactgcga gatcgatggt
     3421 attaaaaatg aaaacattac ctattcgaat cctgaaaatg gaacctacac cgttgcaatt
     3481 gatttgtata gtaaatgtac gtcgggtgtt gaaggtgcaa aatactcagt aacagcagtg
     3541 tatgatggga aagctcttgc gttgtcgaat ccggttggtc agtttgaaga tgataattac
     3601 ggaagtggcg actcagaaag tcaatatgta actattggaa aattcacgat ttcggggagc
     3661 agtaaaagtg ctttgttgga aacagttgat caacgcccgg taaatgataa gcttttattg
     3721 ctgaagcaga aactgttgaa tgctaaggct gccagtagaa aataataaat aatttttttg
     3781 ggactgaagg tttagttggt gcagataagt gcgtattgaa taacctatct accgacatgt
     3841 ttaaaccttc agccccaaat taatcttagt gaaatcttat cgagtatttt ttttacttca
     3901 ctataaatgt ttctgttagg aattactcaa gatcaacttt ccaatgtttt gctatgtctt
     3961 gcagttcttt ctttgtaagg tatgtaactg caccatgttt tggactttta aagtttgtcg
     4021 ttttcattac tccctcatta aaatgtccaa ggtttttata cgttacaaag tctgttgttt
     4081 cgctgaaacc aatgttgctg ggccttgctc cgtacacatc gtacatcagc acatagctgt
     4141 tggtgcccaa acgtttgaat aggttgggag cttcggtcga aactttctcg ggatcgatgc
     4201 ggggggtgtc gattgggtag ccttcgttga ttttatcgga aactgaatgc aagatttttg
     4261 cgcccgaaac ataaaacaac tggtattgat cgcccacctt ggtaatgtcg ccatcgagcc
     4321 cgccaatttc ggtgattcgc ttgggtgttg tttccagttt agtaaaatcg tcgttggcat
     4381 aggcccaata catgtggcac tctttgttgt tgtaacgaat ggtgaaatac accatcattt
     4441 ttccggcttt tgggtcgaaa acggtttcgg gagcccatga acaatcgatg tcgcccagct
     4501 cgggaaatgc cttatcgatg cggagattgg tgtgcgtcca atgaatcagg tcgtacgatt
     4561 tcatcaaaac cagagcgcgg ttgtttcccc aaccatattt ttcggcaggg cgttcaaatt
     4621 cggtgtcacg aaatccggca cgtttgccaa aaatgtgcag gtcggtcatt gccaaataaa
     4681 aggcaccgtc aggtccgcgt gtaatgtgtg ggtcgcgtac gcctttttgt tctgctagct
     4741 gcgatccaat aaagattgca ttgcctccgt tgaggtcggt aaaggtgtag ccgtcggcac
     4801 tgatggccat gtaggccgat tgatcctgat ctttgaaata gaccaacaga tagcctgcga
     4861 gatccttttc atcgaatttc tgctttggtt tctcgagttt tgtaactatt tcaatgcctg
     4921 tagaattagt ttgcaccata ctgttttgag agtatgtatt ttctgataga tgcaatacga
     4981 tcataatcag aataaaatgg taaaatctat ttttcatttc taatcagttt tgaacatcac
     5041 aaaaaaataa tggttcagta ataattaaac tctgaattgt aagtagtttg tattataaaa
     5101 tttaaagagt cttaatactt ctgctatttt ctttcattct taaggtcttc cattaacgct
     5161 ccgagcttgt ttacatagtc ggtaaattca gtggaatatc ttacagaatc atttcgttgc
     5221 tccggcagtt ctatcttcaa atgatgccga atcgatgctg gtcccgagtc aagaataata
     5281 atatcggttg aaagaaaaat agcttcactt tcggagtgtg taacaaaaac aattgttggg
     5341 tcaatcttgg cattttcaaa gattgaacga agaaaaactt gaatttgttt acgcgtaact
     5401 gtatccaatg caccaaaagg ttcgtccata agcagaattt gcggattcac tacgaggtta
     5461 cgcgcaatag caaccctttg tagttgtccg cccgaaagga tagggtattt tgcccatttt
     5521 ttttcatgac cagctaagcc aacaattttt atcatttcca tcgctttgtc gttggcttca
     5581 tccttatcga tccctttcaa tattaagggg agagcaacat tctgtaagac agttttccat
     5641 tcgaaggaag tatattgctg aaataccata gggatccgat ctttttcggt tcgttttttc
     5701 ccatatatat atacatctcc agaggttgga acttgtaagc ctgaaatgta acggagcaaa
     5761 gttgattttc cacaacccga cttgcccata atggtgataa attgcccccg ttcttttatg
     5821 tcttttatat caaaattaaa atctttaaac acaatattgg gcggattgtg ttcgtcataa
     5881 acctgattaa tattaacaag gtttattata tccgtcaaac cagaaggggc gctcttagtg
     5941 gtattttcaa cttcactatc aacatttatt tctgaaacta ccttgttttc tgtggtttct
     6001 tcaatttcac tatcatcctg tttttggaat atgtttcgtt tcataatcag gcgtatttaa
     6061 atttaaatag tttcttgtcg agcagtttca agaatttgtc ctgtacaaat ccgataaaaa
     6121 tgatgaggaa caaaatagcg tataactgat cgacgtgaga tccacgagaa gtagccatat
     6181 aaatcattga acctaaccct ccttgtgcat taaccatttc tgcaattacg atataagtcc
     6241 agctaactgc cacaaggttt attacatcca ttgaaaacct gctcagaacc gatggaatgt
     6301 agaaatgctt taatgtcatc caagggcttg cattaagtgt ccacattgtt tgtttaagcg
     6361 ctttaaggtg gtcatcggtc tcaatatctt taattcgttg ggtgattgca ggcagcaggt
     6421 atacaaaaat accgaacgca aggaattcga ctttaacccc aagcccaata ccaaaaagag
     6481 ccataaatat accggttgtt gcaggtagtg gtgtaaatcg gatcgcttca aattgcttgc
     6541 tgaccaagtg cctgaagaat ggaatgaggc caactacaaa acccaagggg attgccaaag
     6601 ccaaggcttg caggtatccg ccgagattaa taaagataga atatcctgta ttgtacaaaa
     6661 gattacgctc aaccaaaaga gttttgtaac atagaattac atctataggc gagggtagta
     6721 ttgttgaact tatccagcct aatgtagaaa ttatccacca gactataatc aggagaaacc
     6781 atcccagcat agtaatcaac aatgccgttt ttttgtctac ttcgccggca aacgtaaata
     6841 gcttttttat aatttccata ataccgattt gaaaaacgga tttaagtttt attttcccaa
     6901 taactgaaac tcggtacggc ggtttgcagc ctttccttct tctgaattat tgctggcaac
     6961 cggtttgtca ggtccatttc caacaataat aaagcggttc ggatcgaaat tgtaggtctt
     7021 aaccaaataa tcggcaaccg attgtgctct tttacgtgaa agatctctgt tcatttccgc
     7081 tgaaccaaca ttatctgtat ttccctcgat acgaactttc atcccagcaa attcaacaga
     7141 cagttgccct aactgatttt caattttatc tttcatttcg ggagtaagag cccatgatgc
     7201 tgtagcaaag ttgatgatga tcggtttaat ggcaacagcg ggagcttttt catcggcagc
     7261 agttggggtt tcaaacacga tctggccttc agccttgtgg gcatctcctt gtaaatcatt
     7321 tatttcttgg ataattccag gataagaagc ttcagcccaa ggcacaatat tgtttaattt
     7381 gttgccatat cctgttttat aaacgcgggc catttttgtg tagagttgtt gccctgttag
     7441 tcctgtatag tcagtgctta atccaaaaaa attgatgttg tctccataat tggcgaagtg
     7501 aatttttttc attccatcga gaatgatgct tacgtcgtca ggtacatcaa aagctatttt
     7561 ataggtttga gcggctttgg ccattttttc agggtctctc atttcagcgt ttgctacaag
     7621 ccaggccctt gaaagtttca cgaatagatc ttttttcttt tcaagtacgt cttttcgtgc
     7681 aatgaaaccg tccataataa tattcgggag caattcagta cttgtaagaa tctttgcatc
     7741 tcttgatttt aaacactctt catcgtcagg cgaccatacc actgtggcat cgacattgcc
     7801 ggtagtgaaa gcggttttgg cagcaaaagg atcgctcatc ggtagaattt tgacatcttg
     7861 ttcggtgagt ccaccggcct caagggttgc atgcagtaag gtattgctgg gccagcccaa
     7921 ggcacaggct atttttttgc ccttaaggtc tgcaacggta tttattgatt tatcaacaat
     7981 aaaaacatca gctcctcgcg aatcatcaat tttcaagaat tgaacgaccg acatttttgc
     8041 gagatcacta cttctatcca tcccaattgg agaaatatct gtagttgtaa atatgaagtc
     8101 aagatccccg ttcttcaagg catcgttaag tttttgcata tcatccatcc tgatggcttc
     8161 gaaagtaagg ccaaactctg tatacattct tgagtcttta tttggtgcag caccaccgtt
     8221 cataaatatt ccgggggcaa atccaacaaa gttgttaaac ccagcacgta attctgcttt
     8281 atttcctttt gatgatttgt tcaggtatgg gtatccgaaa taaacaccgg cacctactgc
     8341 accaatgatt agtataagta cgaaaagttg aaatccaaat gtccattttt gggggaatcc
     8401 gggaattgat ttttctgttt tgttttgata atcagttgtc attttgctta tattgtttta
     8461 tttgacattc ttgaaaattc actaaaaaat atcaccaagc ggaccagctg atgctttttc
     8521 ttcgctggta agtttgtgat tggggtttga gattcttgaa ggatttggaa tttccatttg
     8581 acccgcatct agtttgttgc caatggcaag aagtttgtca taagcttcat cgctatcaaa
     8641 gttgaaggca atagtgttcc tttcaagatc ttcaaggttt tgagcagtca tggcgagatc
     8701 ttctgaaatt cggccagcta caaattcaag ggcatattcc agcttccagt tttggcctcc
     8761 aggtcccagt atctctttca ggattgctgt tgcaccgcgt gatgcagctg ccatttccca
     8821 atccttcttc ataatgttga tgctttcctc gaagtcttta attttgtttt cgaggagagt
     8881 tgcgccaatg gctaatttac gatcgagatt agcaaaaata tttgaacgtg ttgcgtattt
     8941 ggtggtccat tcaatgtttt tttcgagtat tgtattggtc cgggttccgg ccgaagtggc
     9001 atttataaat tgctgctgca attcaacata ttcatcggtt tctttgacgc tatctccctt
     9061 ttcagctatt attttatcca tctgttgttt tacttcactc gccttttttg ttagccgctg
     9121 aacttcatct ttcgagcttt cagcaacttt ccgggtttca ttcgacattt gttcaaaatc
     9181 ggtacggagt tgtttaattt tagacttgtg ttttaaaaac aaatcatgtg tctcccacat
     9241 tttttgtttc tgttcttcaa gttcatcaaa tgggttccag cggattatgg ctttgtgaaa
     9301 cgaacggaca attcggcgga aaagcttgaa aatagcaggc a
//
LOCUS       DAOPUW010000005         9068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_7679_length_9068_cov_3.377677, whole genome shotgun sequence.
ACCESSION   DAOPUW010000005 DAOPUW010000000
VERSION     DAOPUW010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9068
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7679_length_9068_cov_3.377677"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PKJ43_00215"
     CDS             <1..665
                     /locus_tag="PKJ43_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ71007.1"
                     /translation="IEGYAANVLEKESLEEVKVKILSKVNAIDCLINGAGGNSPKATT
                     QAEQIIPGETDSLSNFFDLQMDGFDHVFNLNFKGTLLPTMVFAKEMIERQDGVILNIS
                     SMNAFRPLTKIPAYSAAKSSINNFTEWLAVHFAKAGVRVNAIAPGFFITNQNRFLMID
                     EATGGPTPRGNKVLNNTPMGRFGEVEELIGTSVYLISDMSKFVTGIVIPIDGGFSAFS
                     GV"
     gene            738..1811
                     /locus_tag="PKJ43_00220"
     CDS             738..1811
                     /locus_tag="PKJ43_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNZ71008.1"
                     /translation="MELKKNCKYALLVPTSMGVRITPANGQPVHCSDTFKMQATSAET
                     NVASIASYLGLPVKVLTTFVKGSPIAQFIKCNLKSRNMDYEGPEIEQGGPWGYRHQFN
                     IADSGVGSRGPRVQNDRAGEVGRTLNVKDFDLDRIFGEEGVQVVHLSGLIGALSPETS
                     NFCLQIARAAKKYGTKIAFDLNFRASFWKGREKELHDIFTEIASVADILVGNEEDFQL
                     CLGIDGPEAGGEGLAAKIDSFKEMISRVKKTFPNASVFATTLRQVISVNEHLWGAIML
                     EGQNWHVVEPRSINVLDRIGGGDGFVGGLLYGILRDWEPEKWVQFGWASGALATTFLT
                     DYAQPADEDMVWSIWQGNARVKR"
     gene            2006..3292
                     /locus_tag="PKJ43_00225"
     CDS             2006..3292
                     /locus_tag="PKJ43_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HNZ71009.1"
                     /translation="MIFFEKGSPLARLTDDDLKEGMFKALDLIGEKKKVLAIPPDYTR
                     LPSNAGILTSFTYQYYKEKLTDILPALGTHSSMTDAQIQHMFPDVPRNLFRDHDWRND
                     VITLGTVPSSYVEEISEGKVSFDWPAQVNRLLKEGGFDLILSLGQVVPHEVVGMANYN
                     KNIFVGTGGSEGINKSHFVGAAYGMERMMGRANTPVRKLFNYATEHFAQDLPIVYVQT
                     VVGVDEEGKLATRGLFIGDDFEVFTKAADLSLKVNFDMVEKPLKKVIVWLDPTEFKST
                     WLGNKSIYRTRMAMADGGELIVLAPALKEFGEDHQIDKLIRKYGYFGTPATLKAVAEN
                     EDLQANLGAAAHLIHGSSEGRFSVTYCPGKNPENLSRKEIESVGFNWGDLDEITKKYD
                     PKKLKNGFNILPDGEEIFFISNPALGLWAFKDRFID"
     gene            3353..4807
                     /gene="gnd"
                     /locus_tag="PKJ43_00230"
     CDS             3353..4807
                     /gene="gnd"
                     /locus_tag="PKJ43_00230"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HNZ71010.1"
                     /translation="MKQKADIGLIGLAVMGENLVLNMESKGFTVAVYNRTVEKVDHFI
                     NGRGAGKKFIGAHSIEELCASLERPRKVMMLVKAGQPVDDFIELIIPHLEPGDIIIDG
                     GNSHYPDTIRRTKYVESKGLLYIGTGVSGGEEGALLGPSMMPGGSPAAWPAVKEIFQA
                     VSAKVEDGSPCCDWVGEGGAGHFVKMVHNGIEYGDMQIICEAYQMMKDLLGLTADEMH
                     EVFKQYNEGDLDSYLIEITRDILAYKDADGSALVEKILDTAGQKGTGKWTGVAALDYG
                     IPLTLIGESVFARCLSAQKDLRVMASKSISGPKVKFDGDKKQFISDIKDALFGAKIIS
                     YAQGYNLMMEAAKENGWNLNYGGIALMWRGGCIIRSVFLGDIKKAFDKNPELPNLLLD
                     PFFKKTVEKAQAGWRRVCATALTNGIPVPALTSALCYFDGFRTERLPANLLQAQRDYF
                     GAHTYERVDKPRGEFFHTNWTGRGGDTASTTYVV"
     gene            4826..6274
                     /locus_tag="PKJ43_00235"
     CDS             4826..6274
                     /locus_tag="PKJ43_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ71011.1"
                     /translation="MSANQLKTKSATKMTNFRWAICGLLFVALSINYLDRQVLSLTWD
                     EFIKPEFHWNDEIYGIITGIFSLAYALFYLFAGRFIDWVGSKKGYLWSIGIWSVGACM
                     HAFCGVFTESVVGLHSYAELVQATGDVAVLISTVSMYAFLFARVVLALGEAGNFPVSI
                     KVTAEYFPKKDRAFGTAIFNSGASVGALLAPVTIPVLAKKFGWEAAFLVIGGLGFLWM
                     GFWLFLYKKPSESKFVNKAELEYIEQDSKEEDAVDESKEEKKIPFLKCFKFRQTWSFA
                     FGKFMTDGVWWFFLFWTPSYLNNQFGIKTSDPLGMGLIFTLYAIVTVFSIFGGKLPTI
                     FINRSGQNPYAARMKAMLIFAFIPLVVLLAQPLGVYSPWLPVLLIALACAAHQSWSAN
                     IFSTVGDMFPKGAIASITGIGGMAGGLGSMLVQLGAGALFVHAENTQMTFLGFTGKPA
                     GYFIVFIFCSVAYLIGWTVMKSLVPKYKLVKF"
     gene            6356..7603
                     /locus_tag="PKJ43_00240"
     CDS             6356..7603
                     /locus_tag="PKJ43_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HNZ71012.1"
                     /translation="MRKISKYSFGVGDRFGHQGKAQLKAFIAAKEQLGIEFTPVWNKS
                     NREHSIIGTKPAEVRKEADEAVAALGWKGDYYVDADHINFSNVDAFVEPSDFFTIDVA
                     DFIGKPAPADEVASYIQAHKKFIGKLVIPGISESFDVTENWLTSFANNYLLAIDEASK
                     VYAYLVEKKGEGNFVPEVSCDETDPPQSPLDMFFILKLMADKNVPAQTIAPRFSGRFN
                     KGVDYVGDLTQFAKEFEQDLLVIDYAVKQFGLPENLKLSVHSGSDKFSIYPIIAKLIK
                     KHNKGIHIKTAGTTWLEEIAGLALADGDGLKLAKEVYALAYEKMDELTVPYASVIDIR
                     LEKLPKPEVVNNWTAEQFGNAIVHNQSCKEYNPDLRQLIHVGYKIAAKKGDVYYKALE
                     EFEETIGKQVFDNIFERHMKLLQ"
     gene            7777..8535
                     /locus_tag="PKJ43_00245"
     CDS             7777..8535
                     /locus_tag="PKJ43_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ71013.1"
                     /translation="MNNSDPGGKSKELGIEIESLKQTITVLKQFNGVVYSNLSHEIRT
                     PLNGILGFSEIINDPDLSSEERQYYTGLIVESSNLLLSIITDVSDIAKISGGTYKVYP
                     VTFDLNDLMFQLYLTYKPIAEKKELQLFLENVISTEFSVETDPEVLQRVLKKLIDNAL
                     KFTRQGWVKFYYKTNQESVTFFVEDTGIGIQEEQRKNLFDRFTTQPVSQSRHLGGTGI
                     DLSLCSGLIKLLGGEISLLPYSGEGTTFKFSIPR"
     gene            8824..>9068
                     /locus_tag="PKJ43_00250"
     CDS             8824..>9068
                     /locus_tag="PKJ43_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71014.1"
                     /translation="MSRFTKGHMETERSRFILRNSISAILWLALIAIAYILFVEFIYV
                     KNPGFWIDKFYGQPLIIYSIYVFSEFFFGLFPPELFML"
BASE COUNT         2606 a         1666 c         2022 g         2774 t
ORIGIN      
        1 tcatagaagg atatgctgca aatgtcctcg aaaaagaatc actcgaagag gttaaagtga
       61 aaattttaag caaggttaat gcaatcgatt gtcttattaa tggggctgga ggaaattctc
      121 ctaaagcaac tactcaggcc gagcaaatta ttcctggaga aaccgattct ctcagcaatt
      181 tttttgattt gcaaatggat ggctttgacc atgtttttaa ccttaatttt aagggaacat
      241 tgcttccaac catggttttt gccaaagaaa tgatcgaaag gcaagatggg gttatcctga
      301 acatttcttc catgaatgct ttccgcccac tcactaagat ccctgcctat tcggccgcaa
      361 aatcatcaat caacaatttt acagaatggc tggctgttca ttttgctaaa gctggtgtgc
      421 gtgtgaatgc catagctccc gggttcttca ttacaaacca gaatcgcttc cttatgattg
      481 acgaagctac aggtggacct actcctcgtg gaaataaagt gctaaacaat acacccatgg
      541 gccgctttgg tgaagttgaa gaacttattg gcacctcggt ttatcttatt tccgatatgt
      601 caaaatttgt aaccggcatt gtaattccaa tagatggcgg gtttagtgct tttagtgggg
      661 tgtagttaga aatagcacca ctgataaatc accttaatct tcaaaaagat aattgattaa
      721 cgacaaaatg aattgaaatg gaacttaaaa aaaattgtaa atatgctctt cttgttccca
      781 ccagtatggg ggtacgtatt actccggcca atggtcagcc agtacactgc agcgatactt
      841 ttaaaatgca ggctaccagt gccgaaacca atgttgccag cattgcttct tacctcggct
      901 tgccggttaa ggtgcttact acttttgtga aaggaagtcc tatcgctcag tttattaaat
      961 gtaacctcaa gagtcggaat atggactatg aaggacctga aattgagcag ggaggccctt
     1021 ggggctaccg gcaccaattc aatatcgccg atagtggagt agggtcaaga ggacctcgtg
     1081 tacagaacga tagggctggc gaagtcggaa gaacccttaa tgtgaaggat ttcgatttgg
     1141 atcgcatttt tggggaagag ggagttcaag tggttcatct ttcagggctt attggtgcac
     1201 tttcacccga aacaagtaat ttctgtttgc aaattgcccg ggcagccaaa aagtatggta
     1261 caaaaatagc tttcgatctt aatttcaggg cttcgttctg gaaaggacgc gaaaaagaac
     1321 tccacgatat tttcactgaa attgcttcag ttgccgatat attagttggc aatgaagaag
     1381 attttcagct ttgtcttgga attgatggtc ccgaagctgg aggagaaggc ctcgctgcaa
     1441 aaattgacag tttcaaagaa atgatcagtc gtgtgaaaaa gacattccct aatgcttcgg
     1501 tgtttgcaac aaccctacgt caggttatta gcgtgaacga acacctttgg ggcgccatta
     1561 tgctcgaagg tcagaattgg cacgttgttg agccccgttc aatcaacgtc ttagaccgta
     1621 ttggtggtgg cgatggattt gttggtggtt tgctttatgg aatcttaagg gattgggagc
     1681 ctgaaaaatg ggttcaattc ggttgggctt caggagcttt ggcaacaaca ttccttaccg
     1741 actatgcaca acctgccgac gaggatatgg tttggagcat ctggcaaggt aacgcccggg
     1801 taaaaaggta atttgctcaa gtcgctaatt ggttctttcg aatatgtcga aacagaaagc
     1861 atttttgtga aatgctttca ttctaagttc tgagaatatc ttacgttgaa aggagtaaat
     1921 tcataattat tttaaatact cgacaagggc attgaatgcg aagatgttgt aaattagatc
     1981 ttaatgaaac aaaaaaacag agttaatgat attttttgaa aaaggttcac cattggcacg
     2041 tttaaccgat gatgatttaa aagaaggtat gtttaaggcc ctcgatttaa ttggcgagaa
     2101 aaaaaaggtt ttggccatac cacccgatta tacccggcta ccttcaaatg ctgggatatt
     2161 aacctcattt acatatcaat attacaagga aaaactcacg gatatattac cggcactggg
     2221 aacccattct tccatgaccg atgctcagat tcagcacatg ttccctgatg ttccccgcaa
     2281 ccttttcagg gatcacgact ggcggaacga cgtgataaca ctgggaactg tcccctcctc
     2341 ttacgttgaa gagatttcgg aaggaaaagt cagtttcgac tggccagcac aagtgaaccg
     2401 gcttttgaaa gaaggtggtt tcgacctgat cctttctctc ggacaggttg ttcctcacga
     2461 agttgttggg atggccaact acaacaaaaa tatttttgtg ggcaccggag gttccgaagg
     2521 aatcaataaa agccattttg taggagcagc ttatggcatg gaacgaatga tgggccgggc
     2581 caatacgcct gtcagaaaac ttttcaacta tgccacagag cattttgcac aggatcttcc
     2641 cattgtctac gttcaaaccg tagttggggt tgatgaggaa ggaaaacttg caacccgggg
     2701 attatttatc ggcgacgatt tcgaagtttt caccaaagct gctgacctct cgctgaaggt
     2761 gaatttcgac atggttgaaa aacctctaaa gaaagtgatc gtctggctcg atcctaccga
     2821 atttaaaagc acctggctgg gcaacaaaag catctaccgc acccgcatgg ccatggccga
     2881 tggaggtgaa ctgattgttc tggcacctgc cctcaaagag tttggcgagg atcatcagat
     2941 tgataaactg atcagaaaat atggatattt cggcactcct gccaccttga aagcggtagc
     3001 cgaaaacgaa gatttacagg caaatcttgg cgctgcagcc catttgatcc acggttcgtc
     3061 ggaaggaaga ttttctgtaa cttattgccc tggaaagaat cccgaaaacc tgagccggaa
     3121 agagattgaa agtgtaggtt tcaattgggg tgacctggat gaaatcacca aaaaatatga
     3181 cccgaagaaa ttgaaaaacg gattcaacat attgcccgat ggcgaagaaa tattttttat
     3241 ttccaaccct gcattggggc tttgggcttt taaagaccga tttattgact aaagttgaca
     3301 acaaaatcga tttgaattaa actattgaaa caaataaaaa gaaatattta tcatgaaaca
     3361 aaaagcagat ataggattaa tcgggttggc tgtaatgggc gaaaacctgg tattaaacat
     3421 ggaaagcaaa ggctttaccg ttgcagttta caaccgcacc gttgaaaaag ttgatcattt
     3481 catcaatggc cgcggtgcag ggaaaaaatt cattggggca cattctattg aagagttgtg
     3541 tgcatcgctt gagcgcccaa gaaaagtaat gatgctggta aaagcaggtc agccggtcga
     3601 cgactttatt gaactgatca ttcctcatct ggaaccgggc gatatcatca tcgacggggg
     3661 aaattctcat tatcccgata ccatccgccg aacaaaatat gttgaaagca aagggttgct
     3721 ttacattgga actggtgttt cgggtggcga agaaggtgca ttactcggtc cttcgatgat
     3781 gcctggtggt tcgcctgctg catggcctgc tgtaaaggaa atttttcagg ctgtatctgc
     3841 aaaagttgaa gacggttctc cctgctgcga ctgggttggt gaaggcggcg ccggccactt
     3901 tgtgaaaatg gttcacaatg gtatcgaata tggcgatatg cagattattt gcgaagctta
     3961 tcaaatgatg aaagatctgc tcggacttac tgccgatgaa atgcacgaag tattcaaaca
     4021 atataatgaa ggtgacctag actcttatct aattgaaatt acccgcgata ttttagccta
     4081 caaagatgct gatggatcag ctcttgttga gaaaatcctt gatacagcag gtcaaaaggg
     4141 tacaggcaag tggaccggtg tagctgctct tgactatggt atcccattaa cgttgattgg
     4201 agagtcggta tttgcacgtt gcttgtctgc tcaaaaagac cttcgtgtta tggcttcaaa
     4261 atccatctct ggtccaaaag taaaattcga tggagataag aaacaattta tttcagatat
     4321 taaagatgcg ttattcggag caaaaatcat ttcgtatgca caaggataca atttaatgat
     4381 ggaagctgcc aaagaaaacg gctggaatct gaattatggc ggtattgccc ttatgtggcg
     4441 tggcggatgt attattcgtt ctgttttctt aggcgatatt aaaaaggctt tcgataaaaa
     4501 tcctgaattg ccaaatcttt tactcgatcc attctttaaa aaaactgttg aaaaagccca
     4561 ggctggctgg cgtagggttt gtgcaactgc attaaccaat ggaattcctg ttcctgccct
     4621 cacttctgca ttgtgctatt tcgacgggtt tagaacggaa aggcttcctg ctaatctttt
     4681 acaagcacag cgcgactatt ttggtgcgca tacttacgag agagtagata aacctcgtgg
     4741 agaattcttc cataccaact ggaccggtcg tggtggcgac actgcatcaa caacctatgt
     4801 tgtttaacct taactaatta ataaaatgtc agcaaatcaa ctaaaaacca aaagtgcaac
     4861 caaaatgacc aatttccgtt gggcgatttg tggtttgctc ttcgtcgcat tgtctataaa
     4921 ctatttggat cgtcaggtgc tttcccttac atgggatgaa tttatcaaac cagaattcca
     4981 ttggaatgat gagatttatg gaattattac cggtatattc tctttggctt atgctctgtt
     5041 ttatcttttt gccggtcgtt ttatcgattg ggtaggttca aaaaaaggat atttgtggtc
     5101 aattggtata tggtcagtag gagcatgtat gcatgccttt tgtggcgtat ttacagaaag
     5161 tgtagttggt ttgcattcat acgccgaatt agtacaggca acaggtgatg tagctgttct
     5221 tatctcaacg gttagcatgt atgctttctt gttcgccagg gtagtccttg cccttggtga
     5281 agctggtaac ttcccggtat ccatcaaggt tactgctgaa tattttccga agaaagaccg
     5341 tgcttttggt acggcaattt ttaactcagg agcttcagtc ggagcattgc ttgcgccggt
     5401 tacgattcct gttttggcta agaaatttgg ctgggaagca gcttttctgg taattggtgg
     5461 attgggattt ttatggatgg gattttggct attcctgtat aaaaagccaa gtgagagcaa
     5521 atttgtaaat aaagccgaat tagaatatat tgaacaagat tcaaaagaag aagacgcagt
     5581 tgatgaatcg aaggaagaaa agaaaattcc attcctgaag tgtttcaaat tccgtcaaac
     5641 atggtctttc gcttttggga aatttatgac agatggcgtt tggtggtttt tccttttctg
     5701 gactccatca tatctgaata atcaatttgg aattaaaact tccgatcctc tcggtatggg
     5761 gcttattttt accttgtatg caattgttac tgttttttca atttttggag gaaagttacc
     5821 tacaattttt attaacagat cagggcaaaa tccttatgct gcgcgaatga aagcaatgtt
     5881 gatttttgca tttattccat tagtcgtatt gttggcacaa ccacttggcg tttattctcc
     5941 ttggcttcct gttcttttga tcgctcttgc atgtgccgca caccagtcct ggtcggcaaa
     6001 tattttctct accgttggcg atatgttccc aaaaggagca attgcgtcaa ttaccggtat
     6061 tggtggtatg gcaggaggtc tgggttctat gcttgttcaa ttaggtgctg gcgcattgtt
     6121 cgttcatgcc gagaatactc aaatgacatt cttaggattt accggtaaac cagctggtta
     6181 ttttattgta ttcattttct gctcggttgc atacctcatt ggttggactg taatgaaatc
     6241 gttagtacca aaatataaac tggtaaaatt ctaaatgaaa tatattgaaa gagattaggg
     6301 tccctttctt tttcaattta gctgctataa ctaatattga ttaaaaaagt cttagatgag
     6361 aaaaataagt aaatattcct ttggcgttgg cgatcgcttt ggccatcagg ggaaagccca
     6421 actcaaagct tttattgcag cgaaggaaca acttggaatt gagtttactc ctgtctggaa
     6481 taaatcgaac cgtgaacatt cgattattgg aacgaagcct gctgaagtca gaaaagaagc
     6541 tgatgaagcg gttgctgctc ttggatggaa aggcgactat tatgtcgatg ccgaccatat
     6601 caactttagt aatgttgatg cttttgtcga accctccgac ttcttcacca tcgatgttgc
     6661 cgattttatt ggcaagcccg ctcctgctga tgaagttgct tcttatattc aggcacataa
     6721 aaaattcatt gggaaactgg ttattccagg catatccgaa tcatttgatg ttacggaaaa
     6781 ttggttgacc agctttgcca acaactatct gctggctatt gatgaagctt caaaagtata
     6841 tgcttatctg gttgagaaaa aaggggaagg aaactttgta cccgaagttt cgtgcgacga
     6901 aaccgatccg ccacaatcgc cactcgatat gtttttcatc ctgaaattaa tggccgataa
     6961 aaatgtgcct gcacaaacca ttgccccacg tttttcggga cggttcaata aaggagtaga
     7021 ctatgtgggc gatctaactc agtttgctaa agagtttgaa caggatctgc ttgtgatcga
     7081 ttacgctgtg aaacaatttg gtttacccga gaacctgaaa ttgagcgttc attcgggaag
     7141 cgacaaattt tcgatctacc caatcattgc caagctgatc aagaagcata acaaggggat
     7201 ccatatcaaa accgccggaa caacatggct ggaggagata gccggtttag ccctggccga
     7261 tggcgatgga ttgaaacttg ccaaagaggt gtatgcgctt gcttacgaaa aaatggacga
     7321 actcactgtt ccctatgctt cagttatcga catcagattg gaaaaactgc ccaagccaga
     7381 agtggtaaac aactggacag cggaacaatt tggaaatgcg attgtacaca atcagagctg
     7441 caaagaatat aaccccgatt tgcgtcagtt gatccatgtg ggatacaaaa ttgctgcaaa
     7501 aaaaggtgat gtatattaca aagcactcga agaatttgaa gaaaccattg gtaaacaggt
     7561 ttttgataat attttcgaac gccatatgaa gttgcttcaa tagttgtttg tgatattttg
     7621 gaaaggtgtt atctggtaag ggtggcacct ttcttttttt tataatactc tttattgttt
     7681 atggtgtttt gattttgaca tgaaggagga atgttttaac tttccgtttc aaatatggtt
     7741 caattttgat tactattgtt ttttaaatta atcctcatga acaactcaga tcccggtggg
     7801 aaaagtaaag agcttggtat tgaaatagag tctttaaagc agacgattac cgtgttaaaa
     7861 caatttaatg gggtggttta ttcaaatttg agccatgaaa ttcgaactcc gttaaacgga
     7921 attttaggct tttctgaaat catcaacgat cctgatcttt cttccgaaga aaggcaatat
     7981 tataccgggt taattgtcga aagcagcaac ttgttgctat ctattattac cgatgtttct
     8041 gatattgcta aaattagtgg aggtacttat aaagtgtacc ctgtaacttt cgatttgaac
     8101 gatttaatgt ttcaattata cttaacctat aaacctattg ccgaaaagaa agaactgcaa
     8161 ctgtttcttg aaaatgtaat aagtaccgag ttttctgttg agactgatcc tgaagtactt
     8221 cagcgtgtgt taaagaagtt gatagataat gcgttgaagt ttactaggca aggttgggtg
     8281 aagttttatt ataaaaccaa tcaagagtcg gttacctttt ttgttgaaga cacaggaatt
     8341 ggtattcagg aagaacaacg aaagaatctt ttcgatcggt tcactactca gcctgtttcc
     8401 cagtcacgcc atttgggagg taccggtatt gatttgtcgt tgtgcagcgg tttgataaaa
     8461 cttcttggtg gcgaaatctc tttgttaccc tattcgggcg aaggaactac gtttaaattc
     8521 tcaattcctc gttgaatttt gctgttaaga ctcttttaat tagctttttt atattctttt
     8581 agaatttcat tattgttgtc gtaaaagagc tttatgttca ggttcatgaa ctaatactct
     8641 gtttgttggt tgtatttact cagaaattat catatcagcc ggataaataa ttaaggagta
     8701 aatactgttt gcgtttgttc ttgtgatgtg atttttgaaa ggatattttt aggttggtaa
     8761 attatagttt ggcaggctga tacgtttgat cagtaatgat tattttttat taccattttc
     8821 taaatgagtc gatttacaaa aggacatatg gaaactgaaa gatcacgttt tattcttcga
     8881 aatagtatat ctgcaatatt gtggcttgcg ttgattgcga ttgcttatat cttgtttgta
     8941 gagtttatct atgttaaaaa ccccggtttt tggattgata aattttatgg gcaaccgttg
     9001 attatttatt ctatctatgt tttttcagaa ttctttttcg gtttgttccc ccctgaacta
     9061 tttatgct
//
LOCUS       DAOPUW010000006         8905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_7864_length_8905_cov_3.843842, whole genome shotgun sequence.
ACCESSION   DAOPUW010000006 DAOPUW010000000
VERSION     DAOPUW010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 8905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8905
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7864_length_8905_cov_3.843842"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..418
                     /locus_tag="PKJ43_00255"
     CDS             38..418
                     /locus_tag="PKJ43_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71015.1"
                     /translation="MKGAGYKNFFRFMVLVLGILFANLVTMWMDNFMLSYKGRFAPYV
                     FTWIGMAIIAIIYYPLFTRVDKWATKLGDKYMKIGKKVAGKEIGSIVAFVAGLFALFY
                     FYGKEWFNTNVFSSFIKAVGKWFS"
     gene            487..1425
                     /locus_tag="PKJ43_00260"
     CDS             487..1425
                     /locus_tag="PKJ43_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNZ71016.1"
                     /translation="MEYITNLHNLEEFQDFDNLELIAREVVEGFITGLHRSPFHGFSV
                     EFSEHRQYNEGESTKNIDWKLYGRTERLYVKQFEEETNLRCHLVVDTSSSMLFPYNKG
                     KSLQMNKLTFSVYCCAALIYMMRRQRDASGLTLFSDKIDFHQNARLSSVHLDLLYSNL
                     GSLLKPESTGLKRTTDAVGVLHQIAENIHKRPLVVIFSDMIESAEPDAMYSALQHLRY
                     NKHEVILFHVTDKGHEEELVYSNRPHKFIDLETGEMIRVKPQEFRQQYAKLMKEYVED
                     LKLKCGQYNIDVVEADINEDFRHVLFSYLIKRGKLA"
     gene            complement(1513..2619)
                     /locus_tag="PKJ43_00265"
     CDS             complement(1513..2619)
                     /locus_tag="PKJ43_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNZ71017.1"
                     /translation="MKNTFYSRESIGSKEKVRVYHIKSSLFSRFSHVNLLIILALMLI
                     SSGCVPMKQFSEMKSKASLYETENEKLKEENRKLTVEMNELDGKLLAMQHKLFELEKE
                     YNSLTKDRAMLQEKNESLQRMQEEFEAQINKLKSGNSDEIAKLLEELQTLQASLIERE
                     NNVKATSNELKLQEEKIKIQEAKLKEAQDLYNQQQAKLLELQNALNKQKDAVESLKGK
                     LNQALRGFYDQGLTVHEKNGKIYVSLEEQLLFQTGSYTVDPKGQEAIKKLSEVLANNT
                     DINILVEGHTDDVPLTGSGQIKDNWDLSVMRATAVTRILLQNKAIDPKRITSSGRGEF
                     VPLDPAKTPEARKKNRRTEIILTPNLNEVLKLIQ"
     gene            2873..3673
                     /locus_tag="PKJ43_00270"
     CDS             2873..3673
                     /locus_tag="PKJ43_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HNZ71018.1"
                     /translation="MINEAVVYQVDAFTDEPFKGNPAGVVILAVEAPLDWMQNIAMEM
                     NLSETAFIFPCKTGFVIRYFTPEREVPLCGHATLASSHIIYELGLKALHETIVFQAKG
                     GELTVKNERGNIVMNFPEYSLQKIETNKDFNRVVGFEPVEMYSSDYGWVIAVSKEEKE
                     IANAKPIVEEMIKSGLGHLMITAKAEFSDADFVLRCFAPVSGINEDPVTGSAHCALTP
                     LWSRKLGKNELESLQLSKRTGRMKVRMCDNRVEIQGKALTIFEIKLKI"
     gene            3727..5625
                     /gene="dxs"
                     /locus_tag="PKJ43_00275"
     CDS             3727..5625
                     /gene="dxs"
                     /locus_tag="PKJ43_00275"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNZ71019.1"
                     /translation="MVSERYTYLNKIDSPADLRKLSVGQLTAVCDELRAFLIEELSVN
                     PGHFGASLGVIELTVALHYAYNTPRDLLVWDVGHQAYGHKVLTGRRDSFHTNRKYKGI
                     SGFPSPKESEYDSFGVGHSSTSISAALGMAVASKLKGDKRKVVAVIGDGALTGGMAFE
                     ALNNACVNNPNILVILNDNHISIDPNVGGINKYLVKITASRTYNQFRNNLWDFLGHFK
                     WLGIKTRRAFQKISYSTKSFFFKESNLFEALNIRYFGPVDGHDVEALVRLINQLKDIP
                     GPKLLHVITKKGKGFTPAEDNQTLFHAPGKFDKYTGKQVISENGDEPPLYQEVFGETL
                     LELAKINHSIVGVTPAMLSGCSMNIMQKEIPHRVFDVGIAEQHAVTFAAGMASAGMVP
                     YCNIYSSFSQRAYDQIIHDVAIQKLNVVMCLDRGGLVGADGATHHGVFDMAFLRCIPN
                     LIIASPMNEEELRNLMYTAQNKPRGPFVIRYPRGRGVMKNWRNSFNEIEIGKGRKLVD
                     GARLAVLSIGHPGNFVSYALNGLDDKCSVAHYDMRFLKPIDEELLHEVCSSFKTIITV
                     EDGVITGGLASAVTEFVNQNNYQVKIVPLGIPDYFVEHGKPEELYRECGFDSEGILKT
                     ISKCLNEE"
     gene            5747..6847
                     /locus_tag="PKJ43_00280"
     CDS             5747..6847
                     /locus_tag="PKJ43_00280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ71020.1"
                     /translation="MIGISSLLYTNRIVNEVSLEERGKVERWAEATRIFATNDNLGSE
                     VNLFLNQVLAANTTIPIIVVDQEGKIEIDGNIKYSPKNRDKVLQRELAKMKARQEPIV
                     IPLGDGTEQYLYYKGSSLLVELQYYPVFQLAVIVIFILVSYIAFNSARNAEQNLVWIG
                     MAKETAHQLGTPISSLIAWVELLKEEDVDPKIITELVKDTDRLNTITERFSKIGSNPE
                     LAYDNLYSVLLNSVIYLKTRVSKKISIKYNFNETDELYLPLSSSLFSWVIENLIRNSV
                     DALEGNDGTIAVNVFENEKDVIIEVTDNGKGIPKSKYKTIFQPGYTTKKRGWGLGLSL
                     SRRIVEIFHKGKIILKSSEPNVATTFKITLKK"
     gene            7231..7773
                     /locus_tag="PKJ43_00285"
     CDS             7231..7773
                     /locus_tag="PKJ43_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNZ71021.1"
                     /translation="MKYLSKYEIAFKGLKEGVHQFEYELDDKFFDMFENSEVKNGSLA
                     AGVVLTKQSTLMIFEFYAKGTVELLCDRCLDVYNQQISCNSKLFVKFGMEDEELSDDV
                     ITISRDEFQINVAQYLYEMVILGLPIKRVHPSDKNGNSQCDPEMIAKLGEYLVDSDIS
                     EEEPIVDERWNELRKIINNK"
     gene            7785..7970
                     /gene="rpmF"
                     /locus_tag="PKJ43_00290"
     CDS             7785..7970
                     /gene="rpmF"
                     /locus_tag="PKJ43_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNZ71022.1"
                     /translation="MAHPKHKHSSSRRDKRRTHDKAVPATIASCSNCGAAVKYHTVCP
                     ECGYYRGKLAIEKEITA"
     gene            8200..8547
                     /locus_tag="PKJ43_00295"
     CDS             8200..8547
                     /locus_tag="PKJ43_00295"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNZ71023.1"
                     /translation="MAKIIDNSLGEVNLISSTTSVTGNIASESDIRIDGTLLGNLDTK
                     GRLIIGANGKIEGDITCKSAEIEGTVKGKIDTEDLLSLKSTASFIGNIITTQLYIEPS
                     ALFQGHCEMRRNK"
     gene            8597..>8905
                     /locus_tag="PKJ43_00300"
     CDS             8597..>8905
                     /locus_tag="PKJ43_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71024.1"
                     /translation="MENSFTKFIKGLSYYTLIIFVVGAILFSTIFRSYFLLILPFVLL
                     FYYISTLVLHKFMLRISQKDISRFSFKFMMLSLIKMFIYIIFGVIYIIIDEENAVTFL
                     I"
BASE COUNT         2656 a         1606 c         1882 g         2761 t
ORIGIN      
        1 tttttataaa aaattgaaaa tcaatttgtt tgtgtagatg aagggggctg ggtataagaa
       61 ttttttcagg ttcatggtgt tggtgttggg cattctgttc gccaacttgg ttaccatgtg
      121 gatggataat tttatgctga gctataaagg ccgttttgct ccttatgttt ttacatggat
      181 tggaatggca ataattgcta ttatatatta cccacttttt actagggtcg acaaatgggc
      241 tactaagctg ggtgataagt acatgaagat aggaaaaaaa gtagcaggta aagaaattgg
      301 ctcgatagtg gcttttgttg ccggtctttt cgctttgttt tatttttatg gaaaagaatg
      361 gttcaatacc aatgttttca gttcttttat caaggcggtt ggaaaatggt tcagctagcg
      421 ttaaaaaaga gccatctgtt tgtcatatcg ttttaaaatc cctattttca gcccatcaaa
      481 ttttgtatgg agtacattac caacttacat aatctcgaag agtttcaaga tttcgataat
      541 ctggaactta ttgcccgaga ggtggttgaa ggattcatta ccggccttca ccgtagccct
      601 tttcatggtt tttcagtcga gttctcggaa catcgtcaat acaacgaagg ggaatcgact
      661 aaaaatatcg attggaaact ttatggccgt acagaaaggc tttatgtgaa gcagttcgaa
      721 gaagaaacaa atttacgctg ccatctggta gttgatactt catcatcgat gcttttccct
      781 tacaataagg gaaagtcgtt acagatgaat aaattaacat tctctgtgta ttgctgtgct
      841 gcccttattt acatgatgcg caggcagcgg gatgcttcgg gcttaactct tttttccgat
      901 aaaattgatt ttcatcagaa tgcgcgcttg tcttcggtac acctcgattt gctttacagc
      961 aatcttggct ctttgttaaa gcccgaatcg actggactaa aacgcacaac cgatgcagtt
     1021 ggggtacttc atcaaattgc cgaaaatatt cacaagcgcc cattggttgt tatattttca
     1081 gacatgatcg agtcggccga gcccgatgca atgtacagcg ctcttcagca tttacggtac
     1141 aataagcacg aagtaatctt atttcatgtt accgataaag gtcacgaaga agaattggtt
     1201 tattcaaacc ggccacacaa gtttattgac ctagaaactg gagaaatgat cagggttaag
     1261 ccgcaggaat tcagacaaca atatgccaag ttgatgaaag agtatgttga agatttgaag
     1321 ctaaaatgtg gccaatacaa tatagatgtg gttgaagctg atatcaatga agatttcagg
     1381 cacgtactat tttcttatct tataaaaagg ggcaaactgg cctaagtcct tccatctgaa
     1441 tggtttcttg tttttcacta aaaacaaaac ccggacgata tccgggttct gcagcaattt
     1501 tatatcagat ttttactgga taagcttcaa cacttcattg agattggggg taagaatgat
     1561 ctctgtacga cggtttttct tacgggcttc cggggtttta gccgggtcaa gcggtacaaa
     1621 ttcccctctt cccgatgagg ttatccgttt ggggtcgatt gctttgttct gcagtaaaat
     1681 acgggtaaca gcagttgcgc gcatcacact caaatcccag ttgtctttta tttgccctga
     1741 tccggtaaga ggcacatcat cagtatgccc ttcgaccaaa atattaatat cggtgttgtt
     1801 ggcaagtact tcgctcagct ttttgatggc ttcttgcccc ttggggtcaa ctgtgtaact
     1861 accagtttgg aagagtagtt gttcttcgag cgatacgtat attttaccat ttttttcgtg
     1921 aacagtaagt ccttgatcgt aaaacccacg caaagcctga ttcaactttc ctttcagact
     1981 ttcaacggca tctttttgtt tattaagagc attctgaagt tctaatagtt ttgcttgttg
     2041 ctgattatat aggtcttggg cttccttaag cttagcttct tgtattttta ttttttcttc
     2101 ctgaagtttc aattcgttac tggtcgcttt aacattgttt tcgcgttcaa ttaaactggc
     2161 ctgcaaggtt tgcagctctt ccagcagttt ggcgatttcg tcgctgttgc ccgacttcag
     2221 tttgttaatc tgtgcttcaa attcttcttg catacgttgc aatgactcgt ttttttcctg
     2281 tagcattgcg cggtcttttg ttagcgagtt atactctttt tcaagttcaa aaagcttgtg
     2341 ctgcatagcg agtaactttc catcgagttc attcatctca acggttagtt tgcggttttc
     2401 ttctttcagc ttttcatttt cggtttcata caatgaggct ttcgatttca tttccgagaa
     2461 ttgtttcata ggcacacagc ccgatgaaat caacataagc gctaaaataa ttagcaaatt
     2521 aacatggctg aaacggctaa ataagctgct tttgatatgg tatactctga ctttttcttt
     2581 actgccgata ctttctctgg aataaaatgt atttttcatc gcaatttttt tagcttacaa
     2641 ataataatac gaggatgtta ccgatataac ttcgcgggtg caaatttatt ttcttcgccc
     2701 gaaacacaaa gatatccatt ttgaaatatt tctgcctaca atgtgcaaaa atcaatatct
     2761 tgtgtttgtc taaaagcgaa gtgcagaaag aggtaaacaa tttgccaagc gaaattgtat
     2821 cttagcagac tgtttaggta attttaagtc ccaacgttga agcgaattga taatgattaa
     2881 tgaggctgtt gtatatcagg tagatgcgtt taccgatgaa ccatttaaag ggaacccggc
     2941 aggggttgta atacttgctg ttgaagctcc tttggattgg atgcagaata ttgccatgga
     3001 aatgaattta tcggaaacag cctttatttt tccctgtaaa acaggatttg taattcgata
     3061 ttttactcct gagcgcgaag ttccgttatg tgggcatgct accttagcca gttcacacat
     3121 aatttatgaa cttgggttaa aagcattgca tgaaactatt gtatttcaag ctaagggagg
     3181 agagttgact gtaaaaaatg aaagaggcaa tattgtgatg aattttccgg aatacagtct
     3241 tcagaaaatt gaaacgaaca aggacttcaa tcgtgtggtt ggtttcgagc ctgttgaaat
     3301 gtactcgtct gattatggtt gggtcattgc agtatccaaa gaagaaaaag aaatagccaa
     3361 tgcaaaacct attgttgagg agatgataaa gagtgggctc gggcacttga tgattactgc
     3421 taaagccgaa ttttcggatg ctgattttgt gttacggtgt tttgccccgg tttccgggat
     3481 taatgaagac ccggttactg gctctgcaca ttgtgcatta acacctttgt ggtcgcgaaa
     3541 acttggaaaa aacgaattag aatcgcttca gctttcgaag agaaccggca ggatgaaggt
     3601 aagaatgtgc gacaacagag tagaaattca aggaaaggca cttactattt ttgaaataaa
     3661 actaaaaata taaaaaggca gctattggaa ttgcagcctc ctgaagtaag aatttaactt
     3721 tagaaaatgg tttcagagcg ttatacatat ttgaacaaga ttgattcacc agccgattta
     3781 agaaaactat ctgttggaca gcttacagca gtttgtgatg agctaagggc ttttcttatc
     3841 gaagaactct ctgtgaatcc gggtcatttt ggggcgagtt taggggttat cgaactcact
     3901 gtagccttac attacgcgta caatactccc cgcgatttgc ttgtttggga tgttgggcat
     3961 caggcgtatg gacataaagt tcttaccggc cgacgtgatt cattccatac aaatagaaaa
     4021 tataaaggaa tcagcggttt tccttctcca aaggaaagtg agtacgattc atttggtgtt
     4081 ggacattcat ctacctctat atcagcagca ttaggtatgg ctgttgcctc aaagttaaaa
     4141 ggtgataaaa gaaaagttgt cgctgtgatt ggagatggtg ccttaaccgg tggaatggct
     4201 tttgaggcct tgaacaacgc ttgcgtaaac aaccccaata tattggtaat cctcaacgat
     4261 aatcatattt ctatcgatcc taatgtgggg ggcattaaca agtatctggt gaaaattact
     4321 gcctcacgta cttataacca gttcagaaat aatctttggg attttctggg gcacttcaaa
     4381 tggctgggta ttaaaacacg aagggcattt cagaagatta gttattctac caagtcattt
     4441 ttctttaaag aaagtaatct tttcgaagcc cttaatataa ggtattttgg gccagtcgat
     4501 gggcacgatg ttgaagccct tgtgcgcttg attaaccagc taaaagatat tcctggtcct
     4561 aaactacttc atgtaattac caaaaaaggg aaaggcttta cgccggccga agacaatcaa
     4621 accctttttc acgcaccagg aaaattcgat aaatacacgg gaaagcaggt gattagtgaa
     4681 aacggcgatg aacccccact ttatcaggaa gtattcggag aaacgctgct cgagctggcg
     4741 aagattaatc attcaattgt aggagtaacc cctgcaatgc taagtggttg ttcaatgaat
     4801 atcatgcaaa aagaaatacc tcaccgggtt ttcgatgtag gtattgccga acagcatgcg
     4861 gtaacttttg ccgcgggtat ggcttctgct ggtatggtgc cgtactgtaa tatctattct
     4921 tcgttttcgc aacgtgctta tgatcagatt attcacgatg tggcgataca aaagttaaat
     4981 gtagtgatgt gcctcgatcg gggaggctta gttggtgccg acggggcaac acatcatggc
     5041 gtttttgata tggcttttct ccggtgtatt ccaaacctga taattgcttc gccaatgaac
     5101 gaagaagagt tgcgcaattt aatgtatacc gcacaaaata aaccacgcgg gccttttgtc
     5161 attcgctatc ccagaggccg tggcgtgatg aaaaactggc gcaattcatt caacgagata
     5221 gaaataggga aggggagaaa acttgtagat ggagccaggt tggctgtttt atctattggt
     5281 cacccgggga attttgtttc ttatgcctta aatgggttgg atgataaatg ttcagtcgcc
     5341 cattacgaca tgcgttttct caagcctatc gacgaagaat tgttacacga agtttgttct
     5401 tctttcaaaa caattattac agttgaagac ggtgtaatta caggcggact ggcaagtgca
     5461 gtgaccgaat ttgttaatca gaacaattac caagttaaaa tagttccatt gggcattcct
     5521 gattactttg tagaacacgg taaacctgaa gaactttacc gtgaatgcgg gtttgacagt
     5581 gaggggattt tgaaaactat cagtaaatgc ctcaacgaag agtagaaaaa tgttggtata
     5641 aaaaaatata attgcacttt tctcgtttaa ttctaaataa accttcgtga tggatatata
     5701 tttcagaaaa aaacgttgga agcagatcat tttcattttt gcaataatga ttggtataag
     5761 ttcgttgctt tataccaacc gtattgttaa tgaagtatcg cttgaagagc ggggaaaagt
     5821 tgaacggtgg gccgaagcaa cccgtatttt tgcaacaaac gataaccttg gcagcgaggt
     5881 gaatcttttt ctcaaccagg ttttggccgc caatactaca atcccaatca ttgttgttga
     5941 tcaggaaggg aaaattgaaa tcgacggaaa cattaaatac agtcccaaga accgcgataa
     6001 ggttttgcaa cgagaactgg caaaaatgaa agccaggcaa gaacccattg tgatcccttt
     6061 gggcgatggg acggagcaat atctttatta taaagggtcg tcacttttgg tcgaactaca
     6121 atattatcct gtatttcagc tggctgtaat tgtaattttc attttggttt cttatatcgc
     6181 cttcaactct gcccggaatg cggagcaaaa ccttgtttgg attggaatgg ccaaagaaac
     6241 cgcccatcag ttgggaactc ctatttcatc gctcatcgca tgggtcgaat tacttaaaga
     6301 agaagatgtt gaccccaaaa ttattaccga gttggtaaaa gacaccgacc ggctcaatac
     6361 cattactgaa cgtttttcaa aaattggatc taatcctgaa ctggcatacg acaacctcta
     6421 ttcagttttg ctgaattcag tcatttatct taaaacccga gtttcaaaaa agataagcat
     6481 aaagtataat ttcaacgaaa ccgatgagct ttatcttccg ctttcgtcgt cgctgtttag
     6541 ttgggtaatc gaaaacctta tccgtaattc ggttgatgca cttgaaggga atgatggaac
     6601 aatagcagtt aatgtttttg aaaatgagaa agatgttata atcgaagtta ccgataatgg
     6661 gaaaggaatt ccaaagtcga agtataaaac tatttttcag cctggttata caaccaaaaa
     6721 acgtggctgg ggcttagggc tctcactttc gagacgaatt gtcgagatat ttcataaagg
     6781 caagattatc ctcaaatcgt cggagccaaa cgtggccaca acatttaaaa tcacattgaa
     6841 gaaatgaaga ttaaatttaa ctcactgccc attgaaaccc ttttacggtt aaatatgatt
     6901 tctattatag taatttgttt ttgcgaggct ttcgcagttt cggttctttt aataaaaggt
     6961 ttagctcctg gcactgagcg gtggcgtttt ttcgtttacc ttacggtagt catcgtaatc
     7021 gcattgtcat tgttggcttc gataggtttg tcaatatatt ttctcaagca aaaaagaaaa
     7081 gtgaaaggct caaagaccag caaaaatgat taaacgcgag ccgtgaattg tatacttaaa
     7141 taacttccat tttaacacgc tcaaaaaaag gcacaaaaat ttaactgttt cctaaaaatt
     7201 tatacttttg caaaactttt taagtgggcg atgaaatatc tttcaaaata tgaaattgct
     7261 tttaaagggc taaaggaagg agttcatcag ttcgagtatg aattagatga caagttcttt
     7321 gatatgttcg agaacagcga agtgaagaat ggctcgttag cagcgggcgt agtacttacc
     7381 aagcaatcga cattgatgat tttcgaattc tatgcaaaag gaacagttga gttgctttgt
     7441 gaccgttgcc tagacgttta caatcagcag ataagctgta atagtaaact ttttgtgaaa
     7501 ttcggaatgg aagatgaaga gctgagtgac gatgtgataa ccatatctcg cgatgagttt
     7561 caaatcaatg ttgctcaata tttatacgaa atggtaattt tggggcttcc tataaaaagg
     7621 gttcacccca gcgataaaaa cgggaacagc caatgcgatc ccgaaatgat tgccaaactt
     7681 ggcgaatatc ttgttgatag tgacatatca gaagaggagc caattgttga cgaacgatgg
     7741 aatgaattaa gaaaaataat aaataacaaa taaagaactg agagatggca catccaaagc
     7801 acaaacactc gagctcaaga agagataaac gcagaacaca cgacaaagcc gtgcctgcaa
     7861 cgattgcctc ttgctcaaat tgcggggcag ccgttaaata ccacactgta tgccccgaat
     7921 gtggctatta cagaggtaaa ttagcaatcg agaaagagat tactgcttaa tcaacaatgt
     7981 aaagcaataa aaagaatcct gttaactaca ggattttttt ttgtccatac gaatagggag
     8041 aatgctttgt tcttggtaca tgctgggcgt aacaagttaa attgctgaag attttctcgt
     8101 ttttttttct atttaataca attaatttgt gccgaatagt gcatgttttt tgagttagca
     8161 attctatttt attaaatttg ctaagcttaa aatcagatta tggcaaaaat tatcgataat
     8221 tcactgggag aagttaacct aatatcttca accacatcgg ttaccggaaa catcgcttcc
     8281 gaatcggata ttcgcattga cggtactttg ttgggaaatc ttgacacaaa gggacggttg
     8341 atcattgggg ctaacggtaa aattgaaggc gatattacct gcaaatcggc ggagatagaa
     8401 gggacagtaa aagggaaaat tgacacggaa gatttgctat cattgaaatc aacggcctct
     8461 ttcattggaa atatcattac aacccagcta tatatcgagc ctagtgccct gtttcaaggc
     8521 cattgcgaaa tgaggagaaa caaatagggt aggtaaaatg cccgaaaaca attaataaag
     8581 ccttatcttt agtggcatgg aaaatagttt cacaaaattt attaaagggc tttcctatta
     8641 cacactcata atatttgtgg taggagcaat actcttttcc actattttca ggtcatactt
     8701 tttgcttatt ctgccgttcg ttctcctttt ctattatatc tcaacccttg tgctccataa
     8761 atttatgctc aggatctctc aaaaagatat ttcacggttt agtttcaaat tcatgatgct
     8821 ttcgctgatt aagatgttta tatatattat ttttggagta atctatatta tcatcgacga
     8881 agaaaatgct gttactttcc tcatt
//
LOCUS       DAOPUW010000007         8182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_8765_length_8182_cov_3.898856, whole genome shotgun sequence.
ACCESSION   DAOPUW010000007 DAOPUW010000000
VERSION     DAOPUW010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8765_length_8182_cov_3.898856"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="PKJ43_00305"
     CDS             <1..1143
                     /locus_tag="PKJ43_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71025.1"
                     /translation="KKQTENHKADISDYLIAAKCYEQQFDFVSAANCYQKALTFDSAN
                     VQTLEGLADANVQLGLKKEALDIYSGLFNADSANIRIAGKYASLFMDVGAYKHAQEIY
                     QRLYAADTNSIYFLRKLITSYYKQEEFQELVSLTQKMISLQPLDVEMRLVLITAYQKA
                     DSTLYAIDELNRLLAIDTTNLTAISKLAFLYFTKLQEYDEALVYYKQLNEMENNSDIT
                     HLTNLGICEFFAGDTEVAAYLLDSLSDVLPTDAMIPFYAGLSYKRQGKPDLALQFLER
                     SAFLAVPAHVADIYHHLGRAYSAKRMFTEAFKAFEKVREIAPDNYMVLYDIAITHEEF
                     NLNRKTALSYYQQFLDKCPNRSSPEAMYAASRMVTIKEQLFFEGKD"
     gene            1414..4122
                     /locus_tag="PKJ43_00310"
     CDS             1414..4122
                     /locus_tag="PKJ43_00310"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNZ71026.1"
                     /translation="MKILFRINYVTEWGQDMWISGSNEVLGNWDESKAVPMTYVGNGN
                     WESEIYFPEEQGFVYKYFLRQNVYEITWEGGKNRSFLPVGFPFVVINDNWQLQIDEES
                     VLFTKLFTQVLFKREGKSPIKSKAKPFNRVRFRVSVPRVNANQVVGLVGNNKALGIWA
                     KPIQMSDEHFPFWSVDVDFKEYHLPIHYKYVIIDKKSGEIGTWEEGEERMLLLDNVDG
                     ELVLHIQNDQHFRFPAGKWRGAGVAVPVFSLRSDEGFGTGEFNDLKKLSLWAREAGLK
                     VIQILPINETIATHSWLDSYPYKAISVFALHPIYLNLDLMGKLDDEKLLDEFEMVKNI
                     LNSNTDVDYVEVMRAKSRYYKLIFDQTWNDVKESAQYKAFFKANSYWLEDYAAFCFLR
                     DRYKTANFRQWDEWAVYDAKKIKKLVDPKAPHFEHIAVHYFIQYHLDKQLQEAIDFGH
                     KQGVAFKGDIPIGISPNSIEAWTQPTLFNLNGQAGAPPDGFAVLGQNWGFPTYNWEVM
                     EKDNFQWWKKRLTALSKYFDAYRIDHILGFFRIWEIPQQAMHGLLGYFRPGLPMEIDE
                     IQNWGIVFDEKRFTKPYIKDHFLNEIFGEYTDEVKSKYLVKKKENYSILSEYDTQKKV
                     YNHFSPDQKSEENISEKDAVIRDGLISLLDEVLFIRDPYSASEAYHPRISMHYSYSYA
                     DLDPHTKMKLNEIYIDFFFHRHEDFWKELAYRKLPSLLESSDMLVCGEDLGMVPDCVT
                     EVMKNLNILSLEIQRMPKNPDIEFAHPADAPYLSVCTTSTHDMSTIRGWWEEARDKTQ
                     RFYNHVLGHLGDAPYFAEPWICNEIIQQHLYSPAMLTIFPIQDLLAIDGVLRWSETHR
                     ERINLPSDEKNKWRYRMILPLEELLKDEEFTNTLKSMINVSGRDGK"
     gene            complement(4231..5508)
                     /locus_tag="PKJ43_00315"
     CDS             complement(4231..5508)
                     /locus_tag="PKJ43_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNZ71027.1"
                     /translation="MKTFKLSIACILAVVSLSTSAQVILDGEFRPRMIIDGGYKTIKL
                     ESDPVFVYISQRSRVNLSYKKNKLETYFSIQDVHVWGDDDMYSSSGMSGNSKGINLYQ
                     AWFTLKPSSHISIKTGRQQFNYDDQRLLASRNWNEYQVTYDAILLNWSKENNKLDLAT
                     SWNSSGSSSPLYPKGKIKTMSFARYEKTSGPFNLSGIALFTGYTKADTLTDIAFTGTY
                     GINLLFQKNDINARITGYYQHNINELNGKISAYCLSLLAQKSFLEKKASLALAADFVS
                     GNDGTNTDNEYLKVQHRFSQFYGNRHGLLGYMDYFTSVPNQGLQDYMLKAEYKFSKST
                     LFQADYHYFRLNNNGYDPVNPSIEINRELGSELDLTLQWKIMSEVSLQAGYSMFVATE
                     TLEKLKNVYNKETKFPQFAYIMISVKPTFFKAE"
     gene            complement(5563..6324)
                     /locus_tag="PKJ43_00320"
     CDS             complement(5563..6324)
                     /locus_tag="PKJ43_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010421615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HNZ71028.1"
                     /translation="MENQTKIKVESLNLSIGNKVILNDINISIPKNKITVILGPSGCG
                     KSTLMKSMNRLTDLYPEMKVSGNIFIDDEDILHTKNDVTEIRKRMGLLSQRPYPLPMS
                     IYKNVAYGLKISGRVNKQLLNKRVEHYLQQASLWNEVKDRLKDPASSLSIGQQQRLCL
                     ARGLAVDPDIILADEPTSALDPISSQEIEKKFLELKGNYTIILVTHILRQAARIADYV
                     VFMYLGEVIEQGPAKEFFSNPQTEITKAYLRGSIN"
     gene            complement(6326..7216)
                     /gene="pstA"
                     /locus_tag="PKJ43_00325"
     CDS             complement(6326..7216)
                     /gene="pstA"
                     /locus_tag="PKJ43_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNZ71029.1"
                     /translation="MKKDSNKKTSFKKQRLKYEEYFFRGVMLLSSCLIAFILGLIIYS
                     ILVKGVPSLSWEMITQTPKGGFYFGKEGGILNAIIGSIYLAFGATFIAFIIGLPISLY
                     MNVWLSHKEKWVNMLRFILDLLWGIPSIVYGAFGFTIMILLGIKASLLAGIIVTALLI
                     LPIMIRAIDEGLKTVPKGLKESSYSLGSTKSELAFKIMLRQCIPGIVTAVLLSFGRAI
                     GDAAAVLFTTGFTDNIPTALNQPTATLPLAIFFQLSSPIPEVKERAYAAAVILTSIIL
                     IISVLSRVLTQKYNKHTIKF"
     gene            complement(7197..8117)
                     /gene="pstC"
                     /locus_tag="PKJ43_00330"
     CDS             complement(7197..8117)
                     /gene="pstC"
                     /locus_tag="PKJ43_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNZ71030.1"
                     /translation="MNWNRIRDNLSPTRLQINKFNEGWMLVWTLFLVVVPLLVVSGLL
                     WKSSLLLENHTLKDVIFSSEWKPMSGKFGFMPFIMSSLWVTLLSLLIAAPICLFSAIH
                     LTQYANRYVLRFMQPVIDILAGIPSVIYGVWGIIAVVPFVAEKIAPLVGVQTTGYNIL
                     SASIVLAVMVIPFILNILIDIINTIPKELTEASLSLGAGKWYTIKHVILRKALPGIIS
                     AMGLGLSRSFGETIAVLMVVGNVAAIPSGIFQPGYPLPALIANNYGEMMSIPQYDSAL
                     MFSALLLMIIVLFFNIGSRFLIMSYEKRFK"
BASE COUNT         2343 a         1560 c         1762 g         2517 t
ORIGIN      
        1 aaaaaacaga cagaaaacca taaggctgat atctccgatt atctgattgc tgcaaaatgc
       61 tacgagcaac aatttgattt tgtttcggct gcaaattgtt accagaaggc tttaactttt
      121 gattcagcga atgttcaaac ccttgaagga ttggccgatg cgaatgttca gcttggtttg
      181 aaaaaagaag cattggatat ttattccggg ttgttcaacg ccgattctgc caatatacga
      241 attgcaggaa agtatgcctc actttttatg gacgttgggg cgtacaaaca tgcccaagag
      301 atttatcaac ggctctatgc tgccgatacc aatagcatct attttcttcg gaaactgatt
      361 acctcatatt ataaacaaga agaatttcag gagcttgtgt ctcttactca aaaaatgata
      421 tctcttcagc cgctagatgt tgaaatgcgt ttggtgctaa ttactgctta ccagaaagca
      481 gacagcacct tgtatgccat tgatgagctg aaccgtttat tggctattga tactacaaac
      541 cttaccgcaa tctcgaaact cgcttttttg tatttcacta agttacaaga atatgatgag
      601 gcattagttt attacaagca actgaacgaa atggaaaata atagcgatat tactcacctc
      661 actaatttgg gtatttgtga gttttttgcc ggagatacag aagttgcagc atatttgctt
      721 gattcgcttt ccgatgttct gcccacagat gccatgatcc ctttttatgc tggactctcg
      781 tacaaacggc aagggaaacc cgaccttgca ttacagtttc tcgaacgatc ggcatttctt
      841 gccgtaccag ctcatgttgc cgatatttat caccatttgg gaagggcgta ttctgccaaa
      901 cgaatgttca cagaagcgtt taaggccttt gaaaaagtgc gcgaaattgc tccggataac
      961 tacatggttt tgtatgatat tgccattacc cacgaagaat tcaacctaaa ccgaaaaact
     1021 gcattgagct attatcaaca gtttttagat aaatgtccca ataggtcatc gcccgaagct
     1081 atgtatgctg ccagccgcat ggttactatt aaggaacaac tgtttttcga ggggaaggat
     1141 tgattttctg atgtttgttt gtgaataccg ggtattccgg ggtgaaccag tatgaattat
     1201 gcttcggagt tttcaaagaa taccagtctt ttttgtgtca actattagct gtatttaatt
     1261 aattcagcaa tagttgtttt attttttatg tgttgcaggc attgcttgta ttatcttcga
     1321 tttaaaataa aaataatcgt tttgtctttt ttttattgaa aaatacaacg aacttgaata
     1381 aataaaacag cgactatata aaaacaaatg atcatgaaaa ttttattccg aatcaattac
     1441 gtgaccgagt ggggacagga catgtggatt tccggttcta atgaggtctt aggcaattgg
     1501 gatgaatcga aagctgttcc aatgacctat gtcggtaatg gtaactggga atctgaaata
     1561 tatttccctg aagaacaagg ttttgtctat aaatactttc tgcgtcaaaa tgtctatgaa
     1621 attacttggg aaggcgggaa aaataggagt tttttaccgg taggttttcc ttttgttgta
     1681 atcaatgaca actggcaatt gcaaattgat gaggaaagtg tactgtttac caaacttttc
     1741 actcaggttt tattcaaacg agaagggaag tcgccgataa aaagtaaggc taaacctttt
     1801 aacagggttc gttttagggt aagcgttccc cgggtaaatg ccaatcaggt tgttggattg
     1861 gtaggtaaca acaaggcgct tggaatatgg gcgaaaccga tacaaatgag cgatgaacac
     1921 ttcccgtttt ggtcagtcga cgtcgacttt aaagagtatc atctgccgat ccattataaa
     1981 tatgtaatca ttgataagaa gagtggagaa ataggaacat gggaagaggg agaggagcgg
     2041 atgctgcttt tagataatgt tgacggagag cttgttttgc atattcagaa tgatcaacat
     2101 tttcgatttc ctgcaggaaa atggagaggt gcaggagttg cagtgcctgt tttctcactt
     2161 cgatctgatg agggttttgg aacgggtgaa tttaacgatt taaagaaact gtccctctgg
     2221 gcccgagaag ctgggttaaa agtaattcaa atcctgccaa ttaacgaaac aattgccacc
     2281 cactcctggc tcgactcgta cccttacaaa gctatttcgg tatttgccct tcaccccatt
     2341 taccttaatc ttgatttaat gggcaaactc gacgatgaga aacttttgga cgagtttgag
     2401 atggtgaaaa atatcttgaa cagcaatacc gatgttgatt atgttgaagt aatgcgggca
     2461 aagtcaagat attacaaact tattttcgat caaacatgga atgatgttaa agagagtgct
     2521 cagtataagg cctttttcaa agccaacagt tattggctcg aagattatgc agccttttgt
     2581 ttccttcgcg accgttacaa aacggcaaat tttcggcaat gggacgaatg ggctgtgtac
     2641 gatgctaaaa aaatcaagaa acttgttgat cccaaagccc cgcatttcga acatatagct
     2701 gtacattatt ttattcaata tcacttagat aaacagcttc aagaggctat tgattttggt
     2761 cataaacagg gtgttgcatt caagggcgat atccctatcg gcattagccc aaacagtatt
     2821 gaagcatgga cacaacctac gctttttaat ttgaatggtc aagccggtgc tccgcccgac
     2881 ggttttgccg tgttgggtca aaattggggt ttcccaactt acaattggga agtgatggaa
     2941 aaagataact tccaatggtg gaaaaaacgg ctcactgcac tgtcgaaata tttcgatgcg
     3001 taccggatcg atcatattct tgggttcttc cgtatttggg aaattccgca gcaagctatg
     3061 cacggtttgt tgggatattt tagaccgggt ttgccaatgg aaattgatga aattcaaaat
     3121 tggggaattg ttttcgacga aaaacggttt accaaacctt atattaagga tcatttccta
     3181 aacgaaatat ttggagaata taccgatgag gtaaagagta aatatttagt gaagaagaaa
     3241 gaaaattatt cgatactgtc tgagtatgat acgcaaaaaa aagtttacaa ccatttctca
     3301 cctgatcaaa agtcggaaga aaacattagt gagaaagatg ctgtaattcg tgacggtctc
     3361 atttctctgc tcgatgaagt attatttatc cgtgacccat atagtgcttc cgaggcttac
     3421 catccgagaa tttctatgca ctattcgtat tcgtatgccg atctcgaccc gcatacgaag
     3481 atgaaactta acgagatata tatcgacttc ttttttcacc gtcacgaaga tttctggaaa
     3541 gagttagcat accggaaatt accatcgttg cttgagtctt ctgatatgtt ggtttgtggt
     3601 gaagacttgg gtatggtgcc cgattgtgtt accgaggtaa tgaagaacct gaacattctt
     3661 agtctcgaaa ttcagcgtat gccaaagaat ccagacattg agtttgccca tccggcagat
     3721 gcaccctact tgagtgtatg cacaacatct acgcacgata tgtctacaat tcgcggttgg
     3781 tgggaagaag cccgggataa aactcagcgc ttttataatc acgtgctggg gcatttgggc
     3841 gatgcgccat attttgccga accttggatt tgtaatgaaa tcattcagca gcatttatat
     3901 tccccggcca tgcttaccat tttccctatt caggatttgc tggctatcga cggagtgttg
     3961 cggtggagtg aaacacatag ggaacgtatc aatttgccaa gcgatgaaaa gaacaaatgg
     4021 cgttaccgaa tgattttgcc ccttgaagag ctgttaaaag acgaagaatt tacgaatact
     4081 ttaaagtcga tgattaatgt ttcggggcgc gatggtaaat aagttgtatt gattgatcta
     4141 tttattgctt tgctgcattt acgcaggtga agtttatcaa aatagaaacg ggtaatgaac
     4201 gggatcagcc catcattacc cgtttctgtg ttactctgct ttgaaaaaag ttggtttaac
     4261 actgatcatt atgtatgcaa attgtgggaa ctttgtttct ttgttgtata cattcttcag
     4321 tttttcgagg gtttcggtgg caacaaacat cgagtagcct gcttgtaacg acacttcgct
     4381 cataattttc cattgcaacg tgagatcgag ttctgaacct aattccctgt taatttcaat
     4441 tgatggattc acagggtcgt acccgttgtt gttgagtctg aagtaatggt aatcggcttg
     4501 aaataaggtc gattttgaga atttatattc ggctttaagc atataatctt gcaagccctg
     4561 atttggtacc gaggtaaaat agtccatata tcccaataag ccatgtctgt tgccataaaa
     4621 ttggctaaac cggtgttgca cttttaaata ctcattatca gtatttgttc cgtcgttgcc
     4681 tgaaacaaaa tcagccgcaa gtgctaagga cgctttcttt tcgagaaatg atttttgagc
     4741 aagcaaagaa aggcaatagg cacttatctt tccattaagt tcgttgatgt tgtgttggta
     4801 atatccagtg attcttgcat taatatcatt cttttgaaac aatagattta tcccataagt
     4861 cccggtaaat gcaatgtctg taagggtgtc ggctttggta taacctgtaa aaagggcaat
     4921 gccactgagg ttgaatgggc ctgaagtttt ttcgtagcgt gcaaacgaca tggtcttaat
     4981 ttttcctttt gggtaaagtg ggcttgaaga tccactcgag ttccacgaag tggccagatc
     5041 gagtttgtta ttttctttgc tccaattaag aagtattgca tcgtaggtta cttggtactc
     5101 gttccagttt cgtgaggcaa gcaggcgctg gtcatcgtag ttgaattgtt gccgtccggt
     5161 tttgatggaa atgtgcgaac taggcttcaa tgtaaaccat gcctgataga ggtttatccc
     5221 ttttgagttg cccgacatac ccgatgaact atacatatcg tcgtcgcccc acacatgtac
     5281 atcttgaatt gaaaagtagg tttcgagttt gttctttttg taacttaaat taaccctcga
     5341 gcgttgagaa atgtatacaa atacagggtc gctttctagt ttaattgttt tgtagccgcc
     5401 atcgatgatc attcttggtc tgaactcacc atcaagtatg acttgggccg aagttgataa
     5461 tgatacaact gcaagtatgc aagcaattga taacttgaat gttttcattt ctattctatg
     5521 gtttttagtg gttgtttatt ttaattctgt gatggtagcg tatcagttga tgcttcctct
     5581 gagataagcc ttggttattt cggtttgggg attactaaag aattcttttg ccggcccttg
     5641 ttctataacc tcgcctaaat acatgaacac cacgtagtcg gcaattcgtg cggcttgccg
     5701 aagaatatga gtcaccaata taatagtata atttcctttc aattcgagga attttttctc
     5761 tatttcctgg ctcgaaatcg ggtcgagggc tgatgtgggt tcgtcggcaa ggataatgtc
     5821 gggatctaca gccaacccac gagcgaggca aagtcgctgt tgttgcccaa tagaaaggct
     5881 cgaggcagga tctttaagcc ggtcttttac ctcgttccaa aggctggctt gttgcaaata
     5941 gtgttcaacc cgtttgttga gtagttgctt gtttaccctg ccgctgattt tcagtccata
     6001 agcaacgttt ttataaatgc tcattggaag cgggtagggg cgttgtgaca gtagtcccat
     6061 gcgtttccga atctcggtta catcattttt tgtgtgaagg atgtcttcgt cgtcgatgaa
     6121 aatattaccc gatactttca tttcggggta caggtcggtt agcctgttca tgcttttcat
     6181 gagtgtactc tttccgcacc ctgaaggacc gaggattacc gtaattttat tcttcgggat
     6241 ggagatgttt atgtcgttta atattacctt gttacctatc gaaaggttga ggctctccac
     6301 ttttattttt gtttggtttt ccatgtcaga atttaatcgt gtgtttattg tatttctgag
     6361 ttagcactcg tgataatacg ctaataatga gtattattga tgtgagaatt accgctgcgg
     6421 cgtacgctct ttctttcacc tcgggaattg gcgatgaaag ctgaaaaaag attgccagag
     6481 gtaaagttgc agtgggttgg ttcagtgcgg tgggaatgtt atcggtaaag cctgttgtga
     6541 acaagaccgc tgctgcatca ccaatggctc gtccgaacga taacaaaact gccgtgacta
     6601 tgcccggaat acattggcgt aacataatct taaaggccag ttccgatttt gtcgatccta
     6661 acgaatagct cgattctttc aaccctttgg gtacagtttt cagcccttcg tcaattgccc
     6721 taatcataat gggtaaaatt agcaaggcag taacaataat cccagctagc aacgaggctt
     6781 ttatcccaag caaaatcatt attgtaaagc caaatgcgcc atacacaatt gatggaattc
     6841 cccataaaag atcgaggata aaccgtagca tattaaccca tttttcttta tggctgagcc
     6901 aaacgttcat ataaagcgat attggaagcc cgatgataaa ggcaatgaac gtagctccaa
     6961 atgccaggta aattgatccg ataatggcat ttaatattcc gccttctttt ccgaagtaga
     7021 accctccttt aggggtttgg gttatcattt cccacgacaa cgaaggaacc ccttttacta
     7081 aaatgctgta tatgatgagt ccaagaataa aggcgatcaa acaacttgag agcagcatga
     7141 ccccacgaaa gaaatactct tcatatttca agcgctgttt tttgaagctt gtttttttat
     7201 ttgaatcttt tttcatagct cataatgaga aaacgtgaac caatattgaa aaacagcaca
     7261 attatcatta acaaaagagc cgaaaacatg agcgccgagt cgtattgggg aattgacatc
     7321 atttctccat agttgttggc aattagtgca ggcagcggat agcccggctg aaatattccg
     7381 cttggaattg cggcaacatt tcccacaacc atcaatactg caatggtttc gccaaatgag
     7441 cgggagagac caagccccat cgcagaaatt attccaggta gtgctttccg taaaattaca
     7501 tgttttattg tgtaccattt tcccgctcct aacgacagtg atgcctcggt aagttctttc
     7561 ggaattgtat tgataatatc gattagaata ttaagaatga aaggaataac cattactgct
     7621 aatactattg aagccgaaag tatgttatat cctgtggttt gtacaccaac cagaggagca
     7681 attttttctg caacaaacgg caccacagct ataatgcccc atacaccgta aataactgaa
     7741 ggaatcccgg caagaatgtc gatcaccggt tgcatgaacc gtaacacata acggttggca
     7801 tactgtgtaa ggtgtattgc agagaaaagg caaattggag ccgcaataag caatgaaagt
     7861 aatgttaccc acaatgaact cattatgaat ggcataaatc cgaacttgcc agacattggt
     7921 ttccattcac tcgaaaatat gacatccttt aacgtgtggt tctcaagcaa cagtgacgat
     7981 ttccacagta gccccgaaac aactagcaag ggtactacta ccagaaaaag agtccacacg
     8041 agcatccatc cctcgttgaa tttgttgatt tgtaagcggg tgggactgag gttatccctt
     8101 atacggttcc aattcaattg tttcagtttt taatttttgc catttcagtt tcaatctttt
     8161 ctttcgagag aggaacataa cc
//
LOCUS       DAOPUW010000008         7414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_9981_length_7414_cov_3.712053, whole genome shotgun sequence.
ACCESSION   DAOPUW010000008 DAOPUW010000000
VERSION     DAOPUW010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 7414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7414
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9981_length_7414_cov_3.712053"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..912
                     /locus_tag="PKJ43_00335"
     CDS             34..912
                     /locus_tag="PKJ43_00335"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71031.1"
                     /translation="MNKTFGYYVLMGITWPMQFFPLRLHYVWSDLFYVILYHLVGYRK
                     EVVALNLKNSFPDKTESERKEIARKFYHNFADMFIETLYFAHASEKKTNRLLHVENIE
                     LIHELLDKGKNIILISGHLGNWEYSHLFRDKLKAKRYYVYKHLESKTFDQYYKKLRSR
                     GAEPLEMAETYRKLYSCVKSGQQYLALFISDQRPLAKELYYWVTFMNQDTPVYTGTEK
                     IARNTNAAVVYTEITRMARGHCRLKLELLKEDIGVPRGYEITDMFMARLEESIRQYPD
                     QYFWTHKRWKYSRTSK"
     gene            991..2028
                     /locus_tag="PKJ43_00340"
     CDS             991..2028
                     /locus_tag="PKJ43_00340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ71032.1"
                     /translation="MKIAIVILNWNGRQQMEKYLPSVIEYSTQDNASVIVADNGSTDD
                     SVAYLSASYPEVKLIQLDKNYGFAGGYNNALRQVDADIFCLLNSDVRVTQGWLNPVSD
                     IFESDASIVALQPKILSDRDHTKFEHAGAAGGFIDKYGYPFCRGRIMDKLETDNGQYD
                     QFSDIFWASGACLFIRAQQFMAVGGFDADFFAHMEEIDLCWRLKNKGFRIVYSSQITV
                     FHWGGATLEYNNPRKLFLNFRNSLWSLYKNYTGKHLRLTMFLRMCIDTFAIAKYLATF
                     DLKNSAAIIKAHLEFYRSLANIKAKRKALQPGIVTTAHREMLKSSIIWNFFALSKKTF
                     SAYHEFSSLGR"
     gene            complement(2001..2729)
                     /locus_tag="PKJ43_00345"
     CDS             complement(2001..2729)
                     /locus_tag="PKJ43_00345"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNZ71033.1"
                     /translation="MEYLILALAPVFAIAFYIYFRDKYEKEPVKKLITAFIAGVLIAL
                     PISLFESFFASFTGQSDGYFKAFWDAFMVASLNEEAFKLAALALLIWKSKDFNEKFDG
                     IVYASFISLGFAAIENVLYVFQHGFSTGIARAITAVPAHAFFGITMGFFFGIAKFYPK
                     KRKKHLWMAFLVPFALHGTYDFILMVGHIYLLALFIPFVIYLWISGFKRMKQMNNASI
                     FRNDIDLGIDFSKVKDYRPNDENS"
     gene            complement(2734..3549)
                     /locus_tag="PKJ43_00350"
     CDS             complement(2734..3549)
                     /locus_tag="PKJ43_00350"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNZ71034.1"
                     /translation="MLEKIKATASFIKSKTGFNGKIAVILGSGLGGFASKVEITTALD
                     YAEIPYFPVSTVEGHSGKLLFGKLGGKEIMVMQGRFHFYEGYPMQQVTFPIRVFKMLG
                     VEYLMVSNAAGGINYSFEVGDIMMITDHINLFGTNPLIGKNIDELGPRFPDMSKAYNP
                     ALCAKAREIAQSQNIVMHEGVYIGGTGPSFETPAEYRFLHTIGGDAVGMSTVPEVIVA
                     NHAGMKVFGMSVITNNGLMIPEEGNKHTEVLDVASLAGPKLEAIFTGLIATIE"
     gene            complement(3506..4585)
                     /gene="lpxK"
                     /locus_tag="PKJ43_00355"
     CDS             complement(3506..4585)
                     /gene="lpxK"
                     /locus_tag="PKJ43_00355"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNZ71035.1"
                     /translation="MIKLFLYPLSLIYRLVVSFRNFMYDSKILKSSDFKLPIISVGNI
                     TVGGTGKTPHAEYIISLLKKEFSVAFLSRGYKRKTKGYLLATMNSTLFDIGDEPVQIK
                     QKFPEITVAVCENRRKGIQKLIEDPTINKDVIILDDAFQHRRVEPSINMLLLDYTQPV
                     FEDTFLPYGRLRESLKAFHRANFIVVTKCPDHLQPIEQRIYKNKLDIRPYQNLFFTSI
                     HYGEITPAEKGLSLFSDDLHRYTVLLITGIGNPGPLVEYVEKQVGKIIHRSYPDHHSF
                     KLSDIEQLKEEYNKIENPRKIIITTEKDMVRIKAVKNLPPDFTGFVYYIPIEIKFLDR
                     SKESFNKKILTHVRENKSNSKFYKK"
     gene            complement(4787..6568)
                     /gene="sppA"
                     /locus_tag="PKJ43_00360"
     CDS             complement(4787..6568)
                     /gene="sppA"
                     /locus_tag="PKJ43_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNZ71036.1"
                     /translation="MKSFLKYTLATMVGFILVIFLWFLIALGMVSAIVASSDKETVLK
                     DNSVLEIKLDGEVVDRSTDNPFENLDIPLLSSTKKIGIDLLKESITKAKNEDKVKGIY
                     LRIDDVSAGYADCEEIRSALVDFKSSGKFIYAYSETYSQKAYYLASVADKIFINPEGS
                     LMFTGISSSTPYFSKALDKLGVEVQVIRHGKFKAAVEPYILDKMSPENKEQIETYIGS
                     IWNNVLGEIATSRNTTVSALNSIADENMYFRHARKLISNNLVDSLVYEDQVLDFLKSK
                     AGIESKKDIPFVTVSELKNVPAKRESKGLAKNKIAVIYAAGEIAMESSSSMSEEGISG
                     LALSKEIREARQDSSIKAIVLRVNSPGGSALASELIWREVKLAAEQKPTVVSMGNLAA
                     SGGYYIACAADSIVANPTTLTGSIGVFGLIPNANELIDGKLGIKNEVVKTNKLSDMPS
                     LTRALTDEEEQIMQEMVENVYSTFVSHVAEGRNMTYEQVDAIGQGRVWTGENALKLGL
                     VDKLGDLDDAIAIAKNMAKLDTYRVVKLPKETDPIEELMKGFSTKMKMSILEKELGSA
                     VKYYKSLESIKNSSGILARIPHGLELE"
     gene            6681..7211
                     /gene="folK"
                     /locus_tag="PKJ43_00365"
     CDS             6681..7211
                     /gene="folK"
                     /locus_tag="PKJ43_00365"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNZ71037.1"
                     /translation="MAEVFLSLGSNIGDRASYLAQSVEEISIHIGLIIKQSALYETAA
                     WGFVSQAFLNQVIFVKTSLSPENLMDKCLEIERKLGRQRVSDSGYAPRVIDIDVLFYN
                     NLVYSSPSIQVPHPLLHERRFVLVPFCEIAPEFIHPELHKSVIELLAECPDLSAVELF
                     STENQSSESSSEISAE"
     gene            complement(7172..>7414)
                     /locus_tag="PKJ43_00370"
     CDS             complement(7172..>7414)
                     /locus_tag="PKJ43_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNZ71038.1"
                     /translation="EKDEDDLRETTSLNWTLDEWKNSWMAKNNTPSVFISATEKTHIE
                     ELKEILYEEVKQIHVSRFPYNDFLYSADISEELSED"
BASE COUNT         2056 a         1456 c         1553 g         2349 t
ORIGIN      
        1 cacattggca ataattgata taaacactta accatgaata aaacgttcgg atactatgtt
       61 ttaatgggca ttacctggcc tatgcaattt tttccattga ggcttcatta tgtatggtcc
      121 gatctcttct atgtaatttt atatcatctt gtcggatacc gaaaagaagt ggtggcatta
      181 aacctaaaga attcttttcc agataaaacc gaaagcgaac gaaaagaaat tgcccggaag
      241 ttttatcaca attttgccga tatgtttatc gagacgttat attttgccca tgccagcgaa
      301 aagaaaacaa accgtttgct tcatgtcgag aatattgaac tgattcacga actactcgat
      361 aaggggaaaa atattattct aatttccggg catttaggca actgggaata ttcgcacctg
      421 ttcagggata aactgaaggc caaaaggtac tatgtttaca aacaccttga aagtaagact
      481 tttgaccagt attataaaaa attacgttcg cggggagccg agccgctcga gatggccgag
      541 acctatcgaa aactttattc ctgtgttaaa agcggacagc aatatttggc tctttttatt
      601 agcgaccaac gccctcttgc gaaagaatta tattattggg ttacatttat gaaccaggat
      661 acgcctgtgt ataccggtac cgaaaagata gcccgcaata ccaatgcagc tgtagtttat
      721 accgagatta cacgcatggc aaggggacat tgccgcttga aacttgaatt attgaaagaa
      781 gatattggcg ttccaagagg atatgaaata acagatatgt ttatggcacg gcttgaagaa
      841 tcgatcaggc aataccccga tcagtatttt tggacccata agcgatggaa atattcccga
      901 accagcaaat agttgtcctg gttttttcat ttttacactg aaaagccgaa cttgatcttt
      961 tgtactttat cattttttaa acaagcgaaa ttgaagatag cgatagtaat attgaattgg
     1021 aacgggcggc aacagatgga aaagtatctg ccttcggtta ttgagtacag cacccaggac
     1081 aatgcttcgg taattgttgc cgataatggc tcgaccgatg actcagttgc atatctttct
     1141 gccagttatc ctgaggtaaa actgattcaa ctcgataaaa attatggttt tgcaggcgga
     1201 tataataatg ccctacgtca ggttgatgcc gatatttttt gtctacttaa cagcgacgtg
     1261 cgtgttactc aggggtggtt aaacccggta tccgatattt ttgagtcaga tgcttccatt
     1321 gttgctcttc aacctaaaat attgtcagac cgcgaccata caaaattcga acatgctgga
     1381 gccgccggtg ggtttatcga taagtatgga tatcccttct gcagggggcg aattatggat
     1441 aaacttgaaa cggataacgg gcagtacgac cagttttccg atattttttg ggcctcgggg
     1501 gcctgtttgt tcatcagggc tcagcagttc atggcggtag gcggatttga tgccgatttt
     1561 tttgcacaca tggaagagat tgatttgtgc tggcggttaa agaacaaagg tttcagaatt
     1621 gtatattcat cacaaataac tgtatttcac tggggtggag ctacacttga atataataat
     1681 ccacgcaaac ttttccttaa tttcaggaat agcttatggt cgctatataa aaattacaca
     1741 ggaaagcatt tgcgactgac catgttcctt cgtatgtgta tcgatacttt tgcaatagct
     1801 aagtatttgg caacattcga tttgaaaaat tcagccgcca ttataaaagc ccatctcgag
     1861 ttttatcgtt cgttagcgaa cataaaggcc aaaaggaagg ctttgcaacc cggtattgtt
     1921 acaacggctc atcgggagat gttgaaatca agtataatat ggaatttctt cgcgttatcg
     1981 aaaaaaacgt tcagcgctta tcatgagttt tcgtcgttag gccgataatc tttcactttg
     2041 ctaaaatcga tgcccagatc gatgtcattt ctgaaaatag aagcattgtt catttgtttc
     2101 atccgcttga aacccgaaat ccaaaggtaa atcacaaagg gaatgaaaag agcgaggagg
     2161 tatatgtgcc ctaccatcaa aataaaatca taggtaccat gtagggcaaa cgggaccaga
     2221 aatgccatcc aaagatgttt cttgcgtttt ttaggataaa attttgctat tccgaagaaa
     2281 aagcccattg taataccaaa gaatgcatgt gcaggaactg ctgtaatggc cctggcaatt
     2341 cctgttgaaa agccatgctg aaaaacgtac agtacatttt ctatcgcggc aaaacccagc
     2401 gagatgaaag atgcatacac gataccatca aatttttcat tgaaatcttt gctcttccaa
     2461 atcaacaggg cgagtgctgc aagtttaaag gcttcttcgt tgagggaggc taccataaat
     2521 gcatcccaga atgctttaaa ataaccatcg ctttggcctg taaagctggc aaaaaacgat
     2581 tcaaaaaggg atattggcag agcgatgaga acacccgcaa taaaagccgt gattaatttc
     2641 tttaccggct ctttctcata cttatccctg aaataaatat aaaaagctat ggcgaaaacc
     2701 ggtgccagtg ccagtattag gtactccatg tgcttattct attgtagcaa tgaggccagt
     2761 aaatatggct tcgagttttg gcccggcaag cgaagcaaca tcaagtactt cggtgtgttt
     2821 attgccttct tcgggaatca tcagcccatt gttggtaatt accgacatgc caaaaacttt
     2881 cattccagca tggtttgcca caataacttc gggtactgtt gacatgccca ctgcatcgcc
     2941 accaatagtg tgcagaaaac ggtattcggc tggcgtttcg aaacttgggc ctgtaccgcc
     3001 aatatatacc ccttcgtgca ttacaatgtt ttgcgactgg gcaatttcgc gtgctttggc
     3061 acaaagtgcc gggttgtatg ccttactcat gtcggggaaa cgtggtccca gctcatcgat
     3121 gtttttgcca atcaacgggt ttgttccgaa taaattgatg tggtcggtaa tcatcattat
     3181 atcacctact tcgaatgaat agttgattcc cccggcagcg ttagaaacca tgagatattc
     3241 aacgccaagc attttgaaaa cccttatagg aaaggtgact tgctgcatag gatatccttc
     3301 gtaaaaatgg aagcgtccct gcatgaccat aatttctttt ccgccaagtt ttccaaagag
     3361 caatttgccg ctatgccctt caacggtcga taccggaaag tagggaattt cggcatagtc
     3421 gagtgctgtg gtaatttcta cttttgaggc aaaaccgcca aggcctgagc cgaggattac
     3481 agctatcttt ccattgaagc ctgttttact ttttataaaa cttgctgttg cttttatttt
     3541 ctctaacatg tgtcaggatt tttttattaa atgattcttt cgatctatca agaaatttta
     3601 tttcgattgg aatatagtat acaaaaccgg taaaatcggg cggcagattt tttactgctt
     3661 taattctcac catatctttt tcagttgtga tgataatttt tcgcggattc tcgattttgt
     3721 tatactcttc ttttagttgt tctatatcgc ttaatttaaa tgagtgatgg tcgggatagc
     3781 tgcgatggat tattttgcca acttgctttt caacatattc aacaaggggg ccggggtttc
     3841 ctatgccagt aattaacagc acggtatatc ggtgtaagtc gtcgctgaaa agggaaagcc
     3901 ctttttcggc tggtgtaatt tcgccatagt gaatgctggt gaagaacagg ttttgatatg
     3961 gcctgatatc aagtttattc ttgtaaatgc gctgttctat gggttgcaaa tggtccggac
     4021 atttagttac cacgataaag tttgcccggt ggaacgcctt tagcgattca cgcaagcgac
     4081 catatggcag gaaagtatct tcgaaaacag gttgggtata atcgagcaaa agcatgttga
     4141 tactaggttc tacgcggcgg tgttggaaag catcgtcgag aataattaca tctttattaa
     4201 ttgttgggtc ttcgattagt ttttgaattc cttttcgccg gttttcacaa acggcaactg
     4261 taatttccgg aaatttttgt tttatctgaa ccggttcatc gccaatgtcg aaaagggtag
     4321 agttcattgt tgccagcaaa tatcctttgg ttttgcgctt ataaccacgg ctgaggaagg
     4381 caacagaaaa ttcttttttc agtaatgaaa taatatattc ggcgtgaggg gttttaccag
     4441 taccacccac agttatgttt cccaccgaaa taataggcag tttgaaatcg ctcgatttca
     4501 gaatttttga atcgtacata aaattccgga acgatacgac cagtcggtaa atcagcgata
     4561 aagggtataa aaaaagttta atcattttaa gtaaaatagt ttcgtaagtt acgaagatgt
     4621 aggtagtata caaaaaaaaa tcccgccgga gcaggatttt tataatgtat ttaacccagt
     4681 tttgctttaa gtgtgtttcg ctggctaaac ccgataattt tcaatgtttt ctttttagcg
     4741 aagctacaat tgttgatttt caaactcagg ttaacaaaag gctcaactat tcaagttcaa
     4801 gtccatgagg aatacgggcc aaaatgccgc tgctgttctt tatcgattcg aggcttttgt
     4861 aatattttac tgcgctgcca agttcttttt caaggataga cattttcatt tttgttgaaa
     4921 agcctttcat cagctcttct atcggatcgg tttcttttgg aagttttacc acgcgataag
     4981 tgtcgagttt tgccatattc ttcgcaattg caatggcatc atcaagatct ccgagcttgt
     5041 caaccaatcc taactttaag gcattttcgc cagtccaaac ccggccttgt ccaatggcat
     5101 caacctgttc gtaggtcata ttcctgcctt cggcaacatg tgaaacaaag gtgctgtata
     5161 cattttccac catttcttgc ataatttgtt cttcttcgtc ggtaagtgca cgggtaagcg
     5221 aaggcatatc gctgagttta tttgttttta ctacctcgtt tttgatgccc agtttgccat
     5281 caattagttc gtttgcattg ggaatcagcc cgaaaacccc gattgaaccg gtaagtgttg
     5341 tggggttggc aacgatagag tcggcggcgc aggcaatgta atatccaccc gaagctgcaa
     5401 ggttacccat cgaaaccacg gtaggttttt gttcggcggc caattttact tcgcgccaaa
     5461 tgagttctga tgccagggca cttccaccag gtgaatttac acgcaacaca atggctttga
     5521 ttgatgaatc ttgtcttgct tcgcggatct cttttgacaa ggctaagccg ctaattcctt
     5581 cttcactcat cgatgaggaa ctttccatgg caatttcacc tgcagcatag ataaccgcaa
     5641 ttttgttctt ggcaaggcct ttcgattcgc gtttggccgg gacattcttc aattccgaca
     5701 ctgttacgaa cggaatatct tttttgcttt cgattccagc tttgcttttt aagaaatcga
     5761 gcacttgatc ttcgtatact aaactgtcga ctaagttatt gcttattagt tttctggcat
     5821 gacggaagta catgttttca tctgcaattg aattgagtgc cgaaactgtg gtattgcgag
     5881 aagttgcaat ttcgccaaga acattattcc aaatactgcc aatataagtt tctatttgct
     5941 ctttgttttc agggctcatt ttatcgagga tgtatggctc aacggcagct ttgaatttac
     6001 cgtggcggat aacttgcact tccacaccta atttgtcgag agctttgctg aaataagggg
     6061 tcgatgacga aatcccggtg aacattaatg aaccttctgg gttgatgaag atcttatcgg
     6121 caaccgatgc caggtaataa gctttctggg aatacgtttc ggaatatgca tagataaatt
     6181 ttcccgacga tttgaaatcg acaagggcag aacgtatttc ttcgcagtcg gcatatccag
     6241 ctgaaacatc atctattctg agatagatcc ctttaacttt atcttcgttc tttgcttttg
     6301 tgatgctttc ttttaataaa tcgatcccga tcttttttgt gctagacagt aatggaatgt
     6361 ctaaattttc gaacgggttg tcggttgagc ggtcaacaac ttcgccgtca agcttaattt
     6421 cgagcaccga attgtctttg agtaccgttt ctttgtctga cgaggcaacg attgctgata
     6481 ccattcctaa tgcaataagg aaccaaagaa agattaccag aataaatcca accattgtcg
     6541 ccaatgtgta tttcaggaag cttttcataa taattagatt ttattgttac tattgttttt
     6601 ttactttttt tattaatatg tagccatatt gttttatttg ttacatttcc agtgaaaaaa
     6661 agttaataat attttttctg atggcagagg tttttctttc tttagggtcg aacattggcg
     6721 acagggcttc ttatttagct cagtcggttg aagaaatatc aattcatatt ggtttgataa
     6781 ttaagcagtc ggctttgtat gagacagctg cctggggttt tgtgtcgcaa gcctttctta
     6841 atcaggttat ctttgtaaag acatctttat caccggaaaa tcttatggat aaatgtctgg
     6901 agatagaaag aaagctcggt cggcagcggg taagcgacag tggctatgct ccacgggtta
     6961 tcgatatcga tgtgcttttt tataataatt tggtttacag tagtccttct atacaagtgc
     7021 ctcacccgtt acttcatgag cgacgttttg tgcttgttcc cttttgtgaa attgctccgg
     7081 aatttattca tccggagttg cataagtcag taatcgaatt gttggccgaa tgccccgatt
     7141 tatctgctgt tgaattattt tctacggaaa atcaatcctc ggaaagttct tctgagatat
     7201 ctgctgaata gaggaaatcg ttatacggga aacggctaac atgaatttgt ttcacttctt
     7261 cgtacagaat ctctttcaat tcttcaatat gagttttctc agtagccgag ataaaaaccg
     7321 aaggcgtatt gtttttggcc atccaactgt ttttccattc atcgagagtc caatttaaag
     7381 aagtcgtttc ccttaggtca tcttcgtctt tttc
//
LOCUS       DAOPUW010000009         7400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_10003_length_7400_cov_3.451464, whole genome shotgun sequence.
ACCESSION   DAOPUW010000009 DAOPUW010000000
VERSION     DAOPUW010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10003_length_7400_cov_3.451464"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..1353
                     /locus_tag="PKJ43_00375"
     CDS             193..1353
                     /locus_tag="PKJ43_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HNZ71039.1"
                     /translation="MNYTYLILAFAGFGLLSTSLSAQNDYLTNKSESVKNFTRMEQTY
                     PVAIVTEPNTTNKPKNVIFLIGDGMGVSQVFSGIVANGGNLHLKNFRNIGFSETQSAS
                     DFVTDSGAGGTALSTGERTYNGAIGVNADTVAIQNIREKAELRGKATGVISTSSITHA
                     TPASFVAHQPNREWLETIAADFLKTNIDVFIGGGSKNFTHRRDKKDLTVDLKQKGYQV
                     AYGIEEIAKINSGKLAGFTSDYHNKSKLEGRGNELPIATSTAINILNNDPDGFFLMVE
                     GSQIDWGGHNNNTGIIVTEMLDFDQAIGVALAFAEKNKETLVIVTADHETGGFSIENG
                     NFTTGSVMGNFTSDDHSGVMVPVFAFGPGADLFRGFLKNTDIPKIISELSGY"
     gene            1673..3946
                     /locus_tag="PKJ43_00380"
     CDS             1673..3946
                     /locus_tag="PKJ43_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNZ71040.1"
                     /translation="MSYENTILSDKDTSVVVYEKFKKLFGDDKSIVVVGMVNPDIFQL
                     DQFNAWYDLEKEIRKIDGVLGVLSLSNSVNLTKNNATHQFDILPLTSEKPKTQAEVDS
                     LKSVIKGLKFFEGLIYNPETEATLMTITLDHEKILDENRTIIINKIKDLSEKFSKENK
                     VKIHYSGMPYILTVTMTLVKRELFIFVIMSILIAALILYFFFRSVRVVLSSLLVVGIS
                     ILWTLGLLALFNFKITILTGVIPSLIVIIAIENCIYILNKYHWEYRLHNDKDLALRNA
                     VQRIGVASLMTNMATALGFAAFILVPSQMLQEFGIVTSISIMVEYALSIILLPIIFSY
                     LPPPSEKHTRHLDNGFFRKIVERIIFLVEHKRRPIYLTSLILLVIGIVGVSKMKTSGK
                     ITDDFQQKDPISIDLKFFEENFGGVMPFEISIDTKKKNGAMAMSTVSKIEKLQKYIDS
                     FPEFSKPISIAELFKFSRQAYYGGDSAKYSMPSSMESSFIMSYLPKNTSKGNTELLNS
                     YIDTTYQITRVNYHIADLPTNRLDSVMAKIQPQIDSLFPAGKYEVKITGNSIVYARGT
                     NFLIRHLFESVTIAIILISLLMALLFSSFRMILVSMIPNIIPLIITAAIMGFTGVPIK
                     PSTIIIFSVALGISVDNAIQYLSRYRHELKVNGGKIKESAINALHEAGFSMIYTSIVL
                     VLGFSVFIVSEFGGTQALGMLISTSLFIAMFFNILVLPSLLLTLDKRVTNKGFTKPII
                     EIYDGDDEEQNTSSVSK"
     gene            complement(3958..4593)
                     /locus_tag="PKJ43_00385"
     CDS             complement(3958..4593)
                     /locus_tag="PKJ43_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNZ71041.1"
                     /translation="MNSLFAIFFTSLVVALSGAIMPGPLLTITISESTRRGAMAGPML
                     IAGHAVLELALVVALLLGLGPLLENEIFFIVIAFVGGLLMLWMALGMFRSLPTLSVSY
                     SGKSEAKGNLLMTGIFMSLANPYWIIWWATIGIGYIVLSQKLGIVGVIVFFAGHIFGD
                     LLWYSAISLAVGKGRKLFTDKIYRMLIAFCALFLVGFSIYLIISGFGKINI"
     gene            complement(4590..4976)
                     /locus_tag="PKJ43_00390"
     CDS             complement(4590..4976)
                     /locus_tag="PKJ43_00390"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HNZ71042.1"
                     /translation="MIAVTCAIIVRNDTILACQRRPNSSHPYEWEFPGGKQENYESPE
                     ECIIREIWEELRVEIEIIKPLTPVSFSYPEQEIYLMPFLCQITAGNPTPVEHHTIRWQ
                     RISMFSDLKWSDADAEIFRINEDNIL"
     gene            5164..>7400
                     /locus_tag="PKJ43_00395"
     CDS             5164..>7400
                     /locus_tag="PKJ43_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNZ71043.1"
                     /translation="MNYKKLNIAVGWLTFLVATITYILTLEPRASFWDCGEFITTAFK
                     LEVGHPPGAPFFMLIGRFFTLFGNAQSAAFFMNVMSALASSFTILFLFWSITHLTKRL
                     ITSDENPSLGQTIAILGSGLVGGLAYAFSDTFWFSAVEAEVYATSSLFTALVFWAILK
                     WENVANEKYSNRWLILICYLAGLSVGVHLLNLLAIPAIALIYYFKKFEPTKKGIFWTL
                     VISAIILGAILYVIIPGVVKVASVFERLFVNGIGMPINSGFFFFVLLLVAAIAYGIYY
                     TLKNNHVLLNTLILGLTVILLGISSYMIIPIRSNAFPPMDQNSPNNAFSMLSYLNREQ
                     YGDRPLFYGQYYNSPLDRNESSNPGKKEWRVKDGKYIVTNEGYEPNYDDEYCTFFPRM
                     WSKSDPRHEQEYKRWGKIKGTKKTVRGQSGETETIIKPTFSENFRFFVNYQINFMYWR
                     YFMWNFAGRQNDIQGHGNKIDGNWISGIKFIDEARLGDQDKLPAKYADNPGRNTYFFL
                     PLLLGILGIAFQYKNGKQGKRDLWVVGLLFIMTGLAIVVYLNQYPLQPRERDYAFAGS
                     FYAFAIWIGMGVAFLYQLLKKFTSETAAASSVTAVALLAVPVLMAAQNWDDHNRAHRT
                     SAPDFGYNYLASVQPNGIIFTNGDNDTYPLWYNQEVEGVGVDKRVCNLSYLQTDWYIK
                     QMQRKAYTSDPLPISFKYDQYVQGKRDIVYIMEDPRMAGKSIELSKALEFIKDDNPKT
                     KITQY"
BASE COUNT         2354 a         1538 c         1386 g         2122 t
ORIGIN      
        1 acggggaatt cgatataaac agtttaaaat agccacaaaa agacaatgat tacactaatc
       61 tattagtcta acaattgtat caaaagaaaa atgttgaacc caaaaaagat aattatattt
      121 gtcgttcacc gaagtttttc taagtgaaaa tttgttattt cacaaaccaa attagtatta
      181 aaaaagattt acatgaatta tacatatctc atccttgcat ttgcaggttt tggacttttg
      241 tcgacaagcc ttagtgctca aaacgattac ctaacaaaca agagcgagtc ggtaaagaat
      301 tttacccgca tggaacaaac ttatcctgtt gcgattgtaa cagaacccaa caccactaac
      361 aaacccaaaa atgttatttt tctgattggc gatgggatgg gcgtatctca agttttttct
      421 gggattgtag ccaatggagg aaatcttcat ttaaagaatt tcagaaatat tggcttttct
      481 gaaacgcaat cggcctccga ttttgttacc gactctggtg ccgggggaac agccctttcc
      541 accggagaaa gaacttacaa cggtgctatt ggggtaaatg ccgatacagt tgctattcaa
      601 aacatacgag agaaagcgga actgagagga aaagcaaccg gagtaatttc aacctcatcg
      661 ataacccacg ccacacccgc atcgtttgtt gcccaccagc caaaccgtga atggctggag
      721 actatcgctg ccgactttct gaaaacaaat atcgatgtgt ttattggcgg cggttctaaa
      781 aacttcaccc atcgcagaga caaaaaagac ttaacagtcg acctaaaaca aaaagggtat
      841 caagtggcat atggcattga agaaattgcg aaaataaaca gcggaaaact tgccggtttc
      901 accagcgact atcataacaa aagcaaatta gaaggccgag ggaacgaatt acccattgca
      961 acatctaccg ccatcaatat tctcaataat gatcccgacg gttttttcct tatggttgaa
     1021 ggctcgcaaa ttgattgggg tggacataac aataatacgg ggataattgt aaccgaaatg
     1081 ctcgatttcg atcaggcaat tggagttgca cttgccttcg ctgaaaaaaa taaagaaact
     1141 ctggttattg taacagctga ccacgaaaca ggaggattct ctatagaaaa tggaaatttc
     1201 actacaggaa gtgtaatggg caactttacg agtgacgacc attcaggcgt aatggttccc
     1261 gtttttgctt ttgggcccgg agctgattta ttcaggggat ttcttaaaaa caccgatatt
     1321 cccaaaatca ttagtgaact atcggggtac taaaccattt tcctaggata gaggaaattt
     1381 atgacatgga actttttctt acacagcaaa cacaaaatgc taacatacaa cacatttaga
     1441 caacaaaagt gttagaaaag caaaaaaata tcgaattcct ggtttaatga tattttaata
     1501 cttcgatatt atactacttt tgtgcattga ttaatatggg aaaattatct ttttattttt
     1561 tttaaaaatg tcattacgga tatcttcatt tattctaaaa aataagattc ttttaatcat
     1621 cctccttact gtttttacct tatgtatggc gttttttggc agaaatgtca agatgtcgta
     1681 cgaaaacacc attctatcag ataaagacac ttctgttgtt gtctatgaaa aatttaaaaa
     1741 actttttggc gacgacaaaa gcatcgtcgt tgttggaatg gttaatcctg acattttcca
     1801 acttgaccaa ttcaatgcat ggtacgatct tgagaaagaa ataagaaaaa tcgatggggt
     1861 gcttggagtg cttagtcttt caaactctgt gaacctcaca aaaaacaatg caacacacca
     1921 gttcgatatt ctaccattaa catccgaaaa gcctaaaaca caagctgaag ttgatagtct
     1981 taaatcagta atcaaaggat taaagttttt cgaagggcta atttacaacc ctgaaaccga
     2041 ggctacgctg atgactatta ccctcgacca cgaaaaaata cttgacgaaa acaggaccat
     2101 cattattaat aaaatcaaag acttatcgga aaaattcagt aaagaaaata aggttaaaat
     2161 ccattattcg ggcatgccgt atatccttac ggttaccatg actttggtaa aacgggagct
     2221 gttcatattc gtcattatga gcatcttaat cgcagccctt atcttgtatt ttttcttccg
     2281 atcggtgagg gtcgttctca gttcgttgct cgtagttgga ataagcatcc tatggaccct
     2341 tggtttactg gctcttttca actttaaaat aaccatactt acaggggtaa tcccatcgct
     2401 catcgttatc attgccattg aaaactgtat ttacattctt aataaatacc attgggaata
     2461 ccggcttcat aacgataaag acctggcatt gcgcaacgca gtgcaacgga ttggggtagc
     2521 atcgctaatg accaatatgg caacagccct tggttttgct gcattcattc ttgttccaag
     2581 ccaaatgtta caggaatttg gcattgtcac atccataagc attatggttg aatacgcctt
     2641 aagtattatt ctgttaccaa tcattttcag ctacttgccc ccaccatctg aaaaacacac
     2701 ccgccacctc gacaacgggt tcttccgtaa aattgtagaa aggattattt tcttggttga
     2761 acataaaagg agaccgattt atctcacctc actcatcttg ttagtaattg gtattgtggg
     2821 cgttagcaaa atgaaaacct ctgggaaaat cactgatgat ttccagcaaa aagaccccat
     2881 ttcaatcgat cttaagtttt ttgaagaaaa cttcggggga gtaatgccat tcgaaatatc
     2941 aatagatacg aaaaagaaaa acggggctat ggccatgtct actgtttcaa aaattgaaaa
     3001 gcttcaaaaa tatatcgatt ctttccctga attttcgaaa ccaatttcca ttgccgaact
     3061 tttcaaattt tcgagacaag cgtattatgg aggcgactct gcaaaatact cgatgccaag
     3121 ttcaatggaa agtagtttca ttatgagtta tttgcccaag aacacaagca aaggaaatac
     3181 tgaattattg aattcatata tcgacaccac gtatcagata acccgggtaa attaccatat
     3241 tgccgacttg ccaaccaatc gtctcgattc tgttatggca aaaattcagc cacaaataga
     3301 ctcgctattt ccggcaggaa aatacgaagt aaaaattaca ggtaacagta ttgtttatgc
     3361 acgtggtaca aatttcctga tccgccatct tttcgaaagt gtaactattg ccattatcct
     3421 catttcactt ctaatggctt tgctgttttc ttcattccga atgatcctcg tttcgatgat
     3481 tcccaacatt atcccgctca ttattactgc agcaattatg ggcttcactg gtgtcccaat
     3541 aaaaccttcg accataataa tttttagtgt tgcccttggt atttctgtcg ataatgccat
     3601 tcagtatctt tcccgttaca ggcacgagct aaaagttaac ggaggaaaaa tcaaagaatc
     3661 ggctatcaat gcgctccacg aagccggctt tagcatgata tataccagca ttgtactagt
     3721 actgggattc agtgtattca ttgtttccga atttggtggc actcaagctt tggggatgct
     3781 aatctctact tcacttttta tcgcaatgtt tttcaatata cttgttcttc cttccttatt
     3841 actaacatta gataagcggg ttacgaacaa agggttcaca aaaccaatta ttgaaattta
     3901 tgacggtgac gacgaagagc aaaacacctc tagtgtttca aaataacgct gaacccgtta
     3961 gatattaatt ttcccgaagc cggaaataat aagataaatc gaaaatccga caagaaaaag
     4021 ggcgcagaaa gcaattagca tgcggtaaat tttatcggta aagagtttcc tcccttttcc
     4081 taccgctagc gaaatggcac tataccaaag caaatcgcca aaaatatgac cagcaaagaa
     4141 gacaataact ccaacaatac caagcttctg tgaaagaaca atgtacccaa taccaattgt
     4201 tgcccaccaa ataatccaat acggattagc cagactcata aaaatacctg tcatcaataa
     4261 attcccttta gcctcgcttt tgccagaata cgaaaccgag agggttggca acgacctgaa
     4321 catacccaat gccatccaga gcatgagtaa ccctccaaca aaagctatta caataaaaaa
     4381 aatctcgttc tcgagtaacg gccccaagcc aagcaacaat gccacaacaa gtgctaactc
     4441 aagaaccgca tgaccggcaa taagcatagg accagccatt gcccccctgc gtgttgattc
     4501 actgatggta atagtaagca aagggccagg cattatggcg ccggaaaggg caactactaa
     4561 cgaagtgaaa aaaatagcaa atagagagtt catagaatgt tatcttcatt aatcctgaag
     4621 atttcggcat ctgcatcaga ccatttcaaa tcggaaaaca ttgaaatgcg ctgccagcga
     4681 atggtgtgat gttcaaccgg ggtaggatta cctgcagtta tttggcaaag aaaaggcata
     4741 agataaattt cctgctcggg ataggaaaaa ctgactgggg ttaagggctt aataatctca
     4801 atttcaaccc ttagttcttc ccaaatctca cgaataatac attcttcggg cgattcataa
     4861 ttttcttgct ttccccccgg gaattcccat tcgtagggat ggctcgaatt tggcctccgt
     4921 tgacaggcta atattgtgtc gtttctgaca attatggcac acgttacagc aatcattgta
     4981 taaaaagatc aataattaga tttggtaaag gtacgaattt agcgttacgt ataaaatgtg
     5041 ctaaaaaaaa attcaaatta cttagtgtga aaagtttcta ctatattcgc agattaattg
     5101 gttattgaac gaaaaagtga gcaataaacc acgcgtcaac aaacttaaat aattcaattt
     5161 cagatgaact acaaaaagct gaacattgca gttggatggt taacatttct ggttgccacc
     5221 attacttata tcttaacctt agaaccccgc gccagtttct gggattgcgg tgaatttatt
     5281 actacagcgt ttaaacttga agttggtcac cccccaggtg ccccattttt catgcttata
     5341 gggaggtttt ttaccctctt tggaaatgca caaagcgctg ctttcttcat gaatgttatg
     5401 tcggcattgg ccagttcgtt tacaatacta ttccttttct ggtcaatcac ccacctcact
     5461 aagagactca ttaccagtga tgaaaaccca tcattagggc aaacaattgc tatccttggc
     5521 agcggtctcg ttggagggtt agcttatgca ttttccgaca cgttttggtt ttcggccgtt
     5581 gaagccgaag tatatgcaac ttcttcgctt tttacagcat tggttttttg ggcaatcctg
     5641 aaatgggaaa atgttgctaa cgaaaaatac tctaaccgat ggttaattct aatatgctat
     5701 ctagcaggtc tttcggtagg agttcactta ctaaacctgt tggccattcc ggcaattgcc
     5761 ctgatttact acttcaagaa atttgagcca actaaaaaag gtattttctg gactttagtc
     5821 atttcagcga tcatcttagg agcaatcctt tatgttatta tacccggtgt tgtaaaagta
     5881 gcgtcagttt tcgaacgctt atttgtcaac gggataggca tgccaattaa ctctggcttt
     5941 ttcttttttg tattattact cgttgccgcc attgcttatg gtatttatta tacgctcaag
     6001 aataatcacg tactgcttaa cacattaata cttgggctca ctgtaatttt gttgggaatt
     6061 tccagctata tgattattcc tattcgctct aacgctttcc cacccatgga ccaaaacagt
     6121 ccgaataatg ctttttccat gctttcttac ctcaaccgtg agcaatatgg cgatcgtcca
     6181 ctattttatg gacaatatta caattcacct ctcgaccgca acgaatcatc aaaccctgga
     6241 aaaaaagaat ggcgcgttaa agacggtaaa tatatagtga caaatgaagg ttacgagcct
     6301 aattacgacg atgaatattg tacattcttc ccgcgtatgt ggagcaaaag tgatccacgc
     6361 cacgaacagg aatataaaag atggggcaaa ataaaaggga ccaagaaaac cgttcgcgga
     6421 caatcaggcg aaaccgaaac aattataaaa ccaacatttt cagagaattt taggtttttt
     6481 gtcaattatc agataaactt tatgtactgg cgttatttta tgtggaattt cgccggaaga
     6541 caaaacgata ttcagggaca tggcaacaaa attgacggaa attggatcag cggcataaaa
     6601 ttcatcgacg aagcccgcct tggtgaccaa gataaactgc ctgcaaaata tgccgataac
     6661 cccggcagaa acacctattt ctttctccca ttgttacttg gtattcttgg tattgccttt
     6721 caatataaaa acggcaaaca aggtaaacgc gacctttggg tagttggatt gttattcatt
     6781 atgacaggcc tggccattgt agtctacctc aaccagtacc cgcttcagcc ccgtgaacgt
     6841 gattatgcat ttgcaggttc gttctatgcc tttgctatat ggattggaat gggggtagct
     6901 ttcttgtatc aacttttgaa aaaattcacc tccgaaacag ccgctgcttc gagcgttacc
     6961 gctgtggctt tattagccgt tccagtactt atggcagccc aaaactggga cgatcacaac
     7021 cgcgcgcacc gcacctctgc tcctgatttc ggatataatt acctcgcttc ggtacagcca
     7081 aacggtatta tttttaccaa cggagacaac gacacctacc ccttgtggta taaccaggaa
     7141 gttgaaggtg ttggcgttga caaaagggtt tgtaacttaa gttaccttca gaccgattgg
     7201 tatatcaaac aaatgcagcg caaggcgtac acctcagatc cattgccaat tagctttaaa
     7261 tacgaccaat atgttcaagg caaacgagat atcgtgtaca taatggaaga cccgcgcatg
     7321 gcaggaaaga gtatcgaact tagcaaagcg ctggaattta ttaaagatga taacccgaaa
     7381 acgaaaatta cacaatatga
//
LOCUS       DAOPUW010000010         7106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_10567_length_7106_cov_3.051766, whole genome shotgun sequence.
ACCESSION   DAOPUW010000010 DAOPUW010000000
VERSION     DAOPUW010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7106
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10567_length_7106_cov_3.051766"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PKJ43_00400"
     CDS             complement(<1..246)
                     /locus_tag="PKJ43_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ71044.1"
                     /translation="MISQTNDQLNLAFDFLQYTGENVFLTGKAGTGKTTFLHNLRIKS
                     PKRMVVVAPTGVAAINAGGVTIHSFFQISLGPQLPFDL"
     gene            complement(278..3652)
                     /locus_tag="PKJ43_00405"
     CDS             complement(278..3652)
                     /locus_tag="PKJ43_00405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNZ71045.1"
                     /translation="MLKNILLALILVIGFQSIASNEQFLFRRISVADGLSNSWVRSAY
                     QDRLGYMWFGTNDGINRFDGKSVKVYRPANDHNVPIGNVVVNDMLDADSESFWLCTEI
                     GLFHFFKSDERIIRDPILPFHPVISSLRDSDSSVWFGSNRGLYRLSADLASKVHYQHN
                     PAEEGSISDDYINTLFEDSKKNIWIGTKHGLNRYDKKTDKFVSFFISGEKKNEFVNDF
                     TTLFEGPDGQMWVGTALDGLYIFTENAGIPVFRKIMDGQISSICADSQQRIWIGMASG
                     GGIAVIDPNSVNSTKLSYVLLKNEPSDFHSLGDNSVFRFFTDRNKDMWILTYGDGVNF
                     FSNRTKDFRIVSQRSRAEESIVDNLVNVFYEEDNYFWVGTEGGLSRLDKKSGKYKHYK
                     YDPNDDQSLASNSVYAIMKDSKGNLWIGTWSGGLHRYNYKTDNFTRYLPDGTPGAIGS
                     ENVFSICEDSKENLWIGTVGGGLNRYDYNTGKFDKYIHNPNDPNSLIGRSMSHVYQTS
                     RGDLLISMFNTLEKYNYQSDNFEHIVSHEVGAAPGSFEGNIQGVFEDSRGNIWITSVS
                     GLRLFNPLDKTHRTYSTIDGLPDNTIQAILEDDKGNLWLSTNNGISKFINGIYAPQKP
                     VFQNFSYLDGLPSNNFKRNAAYKSKQGEMYFGSSKGFVHFHPDSIKLNQQVPEIVFSS
                     FLLLQTSPNANSQFKEISKNINDANKLELHYPNTDFTISFAALNYLNPSNNHYKYKLE
                     GYDTEWIESGNENTATYTNLIEGKYTFMVMGANNDGIWNPIPRQLLIEIFPPWWRSTI
                     FKIFTILFIILTLSFIVVSRIIILNREKSLLEAIIDKRTNELSKLNNLLETKQKIILE
                     QNDELSKHRSNLELLVEDRTKQLAQARAKAEESDRLKSAFLANMSHEIRTPMNAIIGF
                     SNLLVSDGLSEEKREKYCELIKSNSKSLFVLINDIIDISIIEANQLILSKSRFNVQSI
                     LLEVYNYFEMENKNRLVFKYENIGTFEDIYLYNDAVRFRQVIVNLLSNAFKYTDSGSI
                     SMGYVVEGGFIRFYVADTGAGIDTTDHEKVFDHFYKSTRDKVKLYRGTGIGLAICKRL
                     VEQMEGKIWVESTLGKGSVFCFTLPNVENSPNENAS"
     gene            4007..>7106
                     /locus_tag="PKJ43_00410"
     CDS             4007..>7106
                     /locus_tag="PKJ43_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNZ71046.1"
                     /translation="MASKEDIKNKKSRINHPSEYHFNKQTELAHFILEASEIGTWSWN
                     IDTDEIYLDDKWFSIHGHTHDEKNTIDRNTWKSLCHPDDVLLVSKEITRHINGETNFF
                     ETEYRMKHKQGHWIWILDRGKISEFTPDGKPLIIIGSHQDITNRKEKEIELVLEEARL
                     ESLLRISQHPSSLISEMLDYALDQAITLTGSKIGYIYFYDEDTKEFTLNTWSKEVMQV
                     CTIVEPKTIYHLEKTGIWGEVVRQRKPIISNDYTAPNEYKKGYPEGHAPLLRFLSVPT
                     FDQDRIVAVVGVANKYEEYNNSDVRQLTLMMDSVWKIVKRREAEEKLTRANRLYAVIS
                     QINQAIVLNNDRHSLFNRICQIIVEYGKFAMAWIGEPDRETQQIMPVAIAGNDNGYLS
                     FAPKISAANVPEGNGPAGTAFRENRISVCNDIANDPDMAIWRDEALKRNFRSAIGLPL
                     SLYGKPAASITIYSSQINFFNAEEIKLLEEVVSDISFALNAIETNLQHQETISALAES
                     EERFRMFAESSPVGGLIYDSSGNIRFLSPRITKMLGYTPNDIPTLDSWFLKAYPNDDY
                     REFVSEKWFYATNESMKSGEPMQPLEFKVTGKDGKQLDIEFRAEVNPEICFVTLSDIT
                     SRKRAEHLANERMKELSAFYKLSELSEKKPFNLETFYADFVNEFPKSFQYPEITAVQL
                     VVGEKTYSTPNFTGKGEMLISPLIIDSTSIGHLTVTYLEEKPEEAIGPFLFEERLLTD
                     GIAERLSKLTERNYAEEQLIESETKFRSLVNQMQVGLAVHEIILDKNGKPTDYRFIDV
                     NPSFELLTGLKQKDIVGKTVLEILPNTEKIWIEKFGQVALTGQPVTFENYAQELNRYY
                     SVTAYQPRPMQFAVLTEDITKYKLALKDLENSNKKFQFLSQAATKMLSMESLNELYRY
                     ITTSLHQQYPNAVILFLLADEDQNKSWLIDIKGLNSKLFEKTIQIAGFDFTKLEFSIL
                     PSHIGLFKSGRLHLFDKGLAEFAGPQFPKTAAKTIEKLLGINQIYTIGINKGERLYAT
                     IHF"
BASE COUNT         2330 a         1487 c         1307 g         1982 t
ORIGIN      
        1 gagatcgaat ggcaattgtg ggccaagcga aatttgaaaa aacgaatgaa tggtaactcc
       61 acctgcgttg attgcagcca ctcctgtagg tgccacaaca accattcgtt taggtgattt
      121 tattcttaga ttatgtaaaa aagttgtttt cccggttcct gcttttccgg ttagaaaaac
      181 attctcgccc gtgtactgta gaaaatcgaa agcgagattt agctgatcat tggtttggga
      241 aatcatagct tagaatttaa aataataaaa acctacatta agaagcattc tcattaggtg
      301 aattttcaac atttggcaaa gtaaagcaaa acacagatcc ttttccaaga gtagattcaa
      361 cccatatctt tccttccatt tgctcaacca atcgtttgca aatagccagt cctattcctg
      421 ttccccggta tagttttact ttatctctgg tacttttata aaaatggtca aatacttttt
      481 cgtggtctgt tgtatcaatc cctgctccgg tatcagcaac atagaaccga ataaacccac
      541 cttcaacaac atatcccatt gaaattgaac cgctatcggt gtatttaaaa gcattactca
      601 acaaatttac aatcacctgt ctaaatcgta cggcatcatt ataaaggtaa atatcttcga
      661 aggtcccgat gttttcatat ttaaaaacca gcctgttttt attttccatc tcgaaatagt
      721 tgtatacttc gagcaatatt gactgaacat tgaacctcga ttttgataaa ataagctggt
      781 tcgcctcgat aatagatata tcaataatat cgttaatgag tacaaacaac gacttgctat
      841 tgctttttat cagctcgcag tatttctccc gtttttcttc agagagccca tcggaaacca
      901 gcaggttaga gaaaccaata attgcattca ttggtgttcg gatttcgtgg ctcatattgg
      961 ccagaaatgc cgatttaaga cgatcgcttt cctcagcctt tgctctagcc tgggctaatt
     1021 gtttggtacg gtcttcgacc aacaattcaa gattgctcct gtgttttgat aactcatcgt
     1081 tttgctcaag tataattttc tgtttggttt ctaacagatt attgagcttc gaaagttcgt
     1141 tggttcgttt atcaataatc gcttctagca ggctcttttc gcggttaaga ataataatcc
     1201 ttgaaactac aatgaaagaa agagttaaaa taatgaacag aattgtgaat attttgaaaa
     1261 tagttgaccg ccaccacgga ggaaagatct caattaacaa ctgtcggggt attggattcc
     1321 atattccgtc gttattagca cccattacca taaatgtgta cttcccctcg atcaggttag
     1381 tataggttgc cgtattttca ttgccagatt caatccattc tgtatcataa ccttccaact
     1441 tatatttata atggttattt gatggattaa ggtaattgag agccgcaaaa gaaatggtaa
     1501 agtcggtatt gggataatgc aattcgagct tattggcgtc attgatgttt tttgaaattt
     1561 ccttaaactg cgagtttgca ttgggtgaag tttgaagtaa taaaaagctc gaaaatacaa
     1621 tttcggggac ctgttgattc aattttatgc tatcggggtg aaaatggaca aaaccttttg
     1681 aagaaccaaa gtacatttct ccttgtttgc ttttataggc tgcattccgt ttaaaattgt
     1741 tagacggtaa cccatctagg taagaaaaat tttgaaaaac cggtttttga ggagcataaa
     1801 taccattgat aaactttgaa atgccattat ttgtgctcag ccaaaggttt cctttatcgt
     1861 cctcgaggat tgcctgaatg gtattatcgg gcaacccatc gatggttgaa tatgtgcgat
     1921 gagttttatc aagaggatta aaaagcctta gccccgagac acttgtaatc cagatatttc
     1981 ccctactatc ttcaaaaacg ccctgaatat ttccttcgaa tgaacccgga gcggccccaa
     2041 cctcatgaga aacaatatgc tcgaaattat cagattggta attatacttt tcgagtgtat
     2101 taaacataga aatcaataaa tcaccgcgcg atgtttgata aacatggctc atactacgcc
     2161 caatcaaact attgggatcg ttagggttat gaatgtattt atcaaatttc ccggtattgt
     2221 aatcatagcg atttagtccg ccaccaacgg ttccaatcca aaggttctct ttgctatcct
     2281 cacaaatact aaaaacattt tcactgccaa tagcccccgg tgtcccatcg ggtaggtacc
     2341 gggtgaaatt atcggtttta taattgtaac gatgcagccc accagaccat gtaccaatcc
     2401 aaagattccc tttagaatct ttcataatgg catacaccga attcgacgcc aagctttggt
     2461 catcgttggg gtcatactta taatgtttgt attttccact tttcttatcg agccgactca
     2521 agcccccttc tgttccaacc caaaaataat tatcctcttc gtagaaaaca tttaccagat
     2581 tgtcaacaat cgattcttcg gcacggctcc gctgacttac aatgcgaaaa tctttggttc
     2641 ggttgctgaa gaaattaacg ccatcgccat aggtaagaat ccacatatct ttgttcctat
     2701 cggtaaaaaa cctgaaaacg gaattatcac caagcgaatg aaaatcagaa ggttcatttt
     2761 tcaacaatac ataagaaagc tttgtagaat tcacactatt gggatcgata acggcaatac
     2821 ctccccccga ggccatacca atccaaatac gctgctgact atcggcacaa atggatgaaa
     2881 tttgcccgtc cataattttt ctaaaaacag gaatacctgc attctcagta aaaatataca
     2941 atccatcgag ggctgttcca acccacattt ggccatctgg gccttcgaac agagtagtaa
     3001 agtcgtttac aaattcattt tttttctccc cagaaataaa gaaggaaaca aacttgtccg
     3061 tttttttgtc ataccggttc aagccatgtt tggtaccaat ccaaatattc ttttttgagt
     3121 cttcgaaaag tgtattgatg taatcgtcag aaattgaacc ctcttcggca gggttatgct
     3181 gatagtgtac tttagaggcg agatcagctg aaagcctgta taacccgcga ttagagccga
     3241 accaaacaga actgtcacta tcacgcaaag aggatataac cgggtgaaaa ggaagaatag
     3301 ggtcacgtat aatacgttcg tcggatttaa aaaaatgaaa aagcccaatt tcggtgcaca
     3361 accaaaaact ctcactatcg gcatcaagca tgtcattaac aacaacattg ccaattggca
     3421 cattatgatc gttggccgga cggtaaactt taacactttt gccatcgaat cggttaatcc
     3481 catcgttggt accaaaccac atatacccta atctgtcctg ataagcagaa cgtacccaac
     3541 tgtttgacaa cccgtcggca acagaaattc tacggaacaa aaactgttcg ttagaagcaa
     3601 tggattgaaa accgattaca agtatgagag caagtagaat atttttgagc atttacagca
     3661 aattaacccg cttaaattac aatttttttg aattcaagct acctgaatat ttttgttttg
     3721 caagaaaatt gatttttaac ccaaattttc attaaattac taaccagtta taaatataaa
     3781 agacagtgaa aaacctcgag gaaggtgaca taacaaaatc acacagcaag aagaagacta
     3841 gtcgagacgt tgaacaaagc aacataacaa agccctactg caattgctat taccgagaac
     3901 gccgttgaaa ttgaatttgg gtacatatta aaagacgcta tatgctcaag ttctttgtcc
     3961 tcggcagagg tattagagtg ttgtatgaaa gtaaaatttt agaaaaatgg catcgaaaga
     4021 agatattaag aataagaaat caaggataaa ccatccatct gaatatcatt tcaacaaaca
     4081 aaccgaactg gctcatttca tccttgaagc ctcagaaata ggcacctgga gttggaatat
     4141 tgacaccgat gaaatatatc ttgatgataa gtggttttca atacatggac atacccacga
     4201 tgaaaaaaat actattgacc ggaatacatg gaaaagcctt tgccaccctg acgatgttct
     4261 gctggtaagc aaagaaataa cccgacacat caacggtgaa actaatttct tcgagacaga
     4321 atatcgcatg aaacacaagc aggggcattg gatatggatt ctcgaccgtg gaaaaatatc
     4381 cgaattcacc cccgatggta aaccgctaat tatcattgga tcgcaccaag acatcaccaa
     4441 tcgtaaagaa aaagaaattg agctagtact tgaagaagct cggttggaaa gccttctccg
     4501 tattagtcaa cacccctctt ccttaatttc agaaatgctc gattatgcat tagaccaagc
     4561 cattacactc actggcagta agattggata tatttatttt tacgatgagg atacaaagga
     4621 attcaccctc aacacatggt cgaaagaggt gatgcaagtt tgcacaatcg ttgaacccaa
     4681 aaccatctat catctcgaaa aaacaggcat ttggggagaa gttgtacgcc aacgcaaacc
     4741 aataataagc aacgactata ctgccccaaa tgaatataaa aaaggatacc ccgaaggtca
     4801 tgccccatta ctgcgttttt taagcgttcc gacatttgat caagaccgga ttgttgccgt
     4861 tgtgggcgtt gcaaataaat atgaagagta taacaattca gacgtgaggc aactcaccct
     4921 gatgatggat tcggtatgga aaatcgtaaa acgccgcgaa gctgaagaaa aactaacccg
     4981 tgccaacagg ctatatgccg ttattagtca aataaatcaa gccattgtgc ttaacaatga
     5041 tcgccatagc ttgtttaatc gcatttgcca aataattgtt gaatacggaa aatttgccat
     5101 ggcttggatt ggtgaaccag atcgtgaaac acaacagatt atgcctgtag ccattgcagg
     5161 caacgacaac gggtaccttt cgtttgctcc caaaatcagt gcagccaacg tccccgaagg
     5221 aaacgggcca gcagggaccg catttaggga aaaccgtata tccgtttgca acgatatagc
     5281 caatgaccct gatatggcta tttggcgcga cgaagcgtta aaacgcaact tccgttcagc
     5341 cataggactt ccgttgtcgc tttatggaaa accggcagcc agcataacca tctattcatc
     5401 ccaaataaac tttttcaacg ccgaggaaat aaaattactc gaagaagtgg tttcagatat
     5461 aagttttgca cttaatgcca ttgaaaccaa cttgcagcat caggaaacca tatctgcttt
     5521 ggccgaaagc gaagaacggt tcaggatgtt tgccgaatcg tctcccgttg gcggcttgat
     5581 atacgacagc agcggaaaca ttcgttttct tagtccacga attacaaaaa tgttaggcta
     5641 tacccccaac gacatcccca cgctcgacag ttggttttta aaagcctatc caaatgatga
     5701 ctatcgcgaa tttgtatcag agaaatggtt ttatgccacc aacgaaagca tgaaatcggg
     5761 ggaacctatg cagcctcttg aatttaaagt taccggaaaa gatggcaaac aacttgatat
     5821 tgaattcaga gcagaagtaa accccgaaat atgttttgtc accttatctg acattactag
     5881 cagaaaacgt gctgagcatt tagccaacga acgaatgaaa gaattaagcg cattttacaa
     5941 gctgtccgaa ctttcagaaa agaaaccttt taacctcgaa acattctatg cagattttgt
     6001 taacgaattt cccaaaagct tccaatatcc agaaatcaca gcagttcaac tggttgtagg
     6061 cgaaaaaaca tactccactc ccaatttcac tggaaaagga gaaatgttaa tttcaccatt
     6121 gatcattgat tcaacgtcaa ttggccattt aacagttacg tacctcgaag aaaaacccga
     6181 agaagccatt ggccccttcc tgtttgaaga acgcttgctc accgacggta ttgcagaacg
     6241 tctttcaaaa ctaactgagc ggaattatgc tgaagaacaa ctaattgaaa gcgagactaa
     6301 attcaggagc ttagtgaacc aaatgcaagt tggcttagca gttcatgaaa ttatcctcga
     6361 caaaaacggc aagccaaccg actatcgatt tattgatgta aatcctagtt ttgaacttct
     6421 taccggatta aaacaaaaag atattgttgg aaaaacggtg ttggaaatat tgcccaacac
     6481 agaaaaaata tggatagaaa aattcgggca ggtggcactg accgggcagc ctgtaacctt
     6541 cgaaaattat gctcaagaac ttaatcgcta ctatagcgta acagcctatc aaccccggcc
     6601 aatgcaattt gccgttttaa cagaagatat taccaagtat aaacttgcat taaaagatct
     6661 tgaaaatagc aacaaaaagt ttcaattttt gagccaggct gccactaaaa tgctttcgat
     6721 ggaaagccta aatgagctat accgctacat tacaacatca ttgcatcaac aatatcctaa
     6781 tgcagtaatt ctgttcttgc tcgctgacga agatcaaaat aaatcatggc taattgacat
     6841 taaaggtctt aacagcaagc tattcgaaaa gacaatacaa atagcaggat tcgattttac
     6901 taagctcgaa tttagcattt taccatcaca catcggttta tttaaatcgg gcagattaca
     6961 ccttttcgac aaaggccttg cagaatttgc aggcccgcaa ttccctaaaa ccgccgcgaa
     7021 aaccatagaa aaactccttg gaatcaatca aatatacact atcgggataa ataaaggaga
     7081 aagattatat gcaacaatcc acttct
//
LOCUS       DAOPUW010000011         7041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_10670_length_7041_cov_3.426711, whole genome shotgun sequence.
ACCESSION   DAOPUW010000011 DAOPUW010000000
VERSION     DAOPUW010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10670_length_7041_cov_3.426711"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..774
                     /locus_tag="PKJ43_00415"
     CDS             196..774
                     /locus_tag="PKJ43_00415"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNZ71047.1"
                     /translation="MSFELPKLNYSLDALEPFMSKQTLEFHYGKHHQAYVNNLNGLIP
                     GTEFENATLEEIVMKSSGGIFNNGAQVWNHTFFFNQFSKDGCTEPRDLLKDAIEKAFG
                     SFDAFKEQFTKAAVTLFGSGWAWLVVNQQGELEIVQTSNAANPMRDGKKPLLTCDVWE
                     HAYYLDKQNLRPKYVEDFWKIVDWKIIAERYQ"
     gene            883..1056
                     /locus_tag="PKJ43_00420"
     CDS             883..1056
                     /locus_tag="PKJ43_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71048.1"
                     /translation="MSKEKVLAVMKSAGKPLKAGEIAELAGLDKKEVDKAMKQLKDAG
                     EIVSPKVCFWQPK"
     gene            complement(1163..2086)
                     /locus_tag="PKJ43_00425"
     CDS             complement(1163..2086)
                     /locus_tag="PKJ43_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNZ71049.1"
                     /translation="MGTKNILFAASVLLLLVSSCKPFYECGQAKPEKKLLVSKRVRTV
                     IEERDYLCKTVEKRDQEIVRLKTDISDLKNEIAGLTNEKAALNQSVTEIEKKYNDLMS
                     QSLSQSEQFNLALKQKTEELAQKERLLQEREMSLDELRQVISRQDSITKRLNDVLRNA
                     LLGFQTDELSVEVKNGKVYVSMSDKLLFKSGSAAVEAKGKEALKALSDVLLKNPEINI
                     LVEGHTDNIPIKTAIYRDNWDLSVARATSIVRILTDEHKIEPIRLTASGKGEYSPRAL
                     NDTPEGRASNRRTEIILSPKLDEVMNLLKVQ"
     gene            complement(2263..3639)
                     /gene="xylE"
                     /locus_tag="PKJ43_00430"
     CDS             complement(2263..3639)
                     /gene="xylE"
                     /locus_tag="PKJ43_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HNZ71050.1"
                     /translation="MKQNKFFIIVVTFVATIGGLLFGYDTAVISGAEKSVQAFLITSQ
                     GLSTFVHGATISSALIGCIIGGAISGLFASRFGRRKSLIIASVMFFLSALGSGFPEFL
                     FFPKGEASIGLLLMFNFYRILGGVGVGMASAIVPMYIGEISPAEIRGRLVSLNQFAII
                     FGMLVVYFVNWGIANGKPIEWINDIGWRWMFLSEAIPAGLFGILLFFVPETPRYLSLT
                     KRDDEALKVLTKINGPEKAKLILAEIKDTVEHSSGKVFSYGKTVIIIGILLSVFQQFV
                     GINVALYYAPRIFESMGAAKDASMMQTVVMGLVNVAFTVLAILTVDKWGRKPLLMIGS
                     LGMAVGMIAISALAFMEIIGISTLVFIIVYTASFMMSWGPICWVLISEIFPNKIRGKA
                     VAIAVAAQWAANYLISSTYPSMMEFSGGMTYGFYALMSILSFIFVWKMVPETKGKTLE
                     EMEHVWKK"
     gene            complement(3859..5175)
                     /gene="xylA"
                     /locus_tag="PKJ43_00435"
     CDS             complement(3859..5175)
                     /gene="xylA"
                     /locus_tag="PKJ43_00435"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HNZ71051.1"
                     /translation="MSAKVYFPSVGQIKFEGKESKNPLAFRYYNPEKVVYGKTMAEWF
                     KFSMAWWHTLCADGGDPFGGQTQKHPWVGAADALQAAKEKMDAGFEFMQKIGIKYYCF
                     HDIDLISEGSSIEEYEANLKAIVAYAKQKQAETGITLMWGTANVFSNARYMNGASTNP
                     NFDAAARAMLQIKNSIDATIELGGKAYVFWGGREGYMSLLNTNMKREKQHMGTMLKMA
                     RDYGRSKGFKGVFLIEPKPMEPMKHQYDVDAETVIGFLKEFGLENDFKLNIEVNHATL
                     AGHTFEHELQCAADAGMLGAIDANRGDVQNGWDTDQFPVDIFELTQAMMVVLQAGGMQ
                     GGGTNFDAKIRRNSTDNEDLFLAHIGAMDVMARALETAAAILEESPYKKMVADRYASY
                     DSGKGKEFEEGKLTFEQVYEYAKANGEPKQISGKQELYEAIVNMYI"
     gene            complement(5247..6731)
                     /locus_tag="PKJ43_00440"
     CDS             complement(5247..6731)
                     /locus_tag="PKJ43_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNZ71052.1"
                     /translation="MYFLGYDVGSSSVKASLINGETGECVASDFFPKQEMKITAQKAG
                     WAEQHPDLWWDNLKLATQSVMASSEIDAKEIEAIGISYQMHGLVVVDKNNNVLRPSII
                     WCDSRAAVIGDKAFETLGGERCLGSLLNSPGNFTASKLMWVKENEPNVYAQIDKIMLP
                     GDYIALRLTGQARTTISGLSEGMFWDFQKNEVSKMIMDHYGFNSSMIPEIVPTFSEQG
                     EVTAEVAKELGLAAGTKVTYRGGDQPNNALSLNVLNPGEIATTAGTSGVVYGVSGEVK
                     YDPYSRVNTFAHVNYTTDLNRLGVLLCINGTGILNSWMNKNVGNNAYNYAEMNELASK
                     VAIGSDGVSILPFGNGTERVLRNKNIGSQISGISFNSHSQAHLFRAAQEGIVFSFNYG
                     MEIMKQIGIDAKVIRAGKANMFLSPIFRETLAGISGATIELYNTDGSVGAARGAAVGS
                     GYYKSFAEAFGSLKKLETIEPNLAKKAEYEAAYESWKALLGKHI"
     gene            complement(6760..>7041)
                     /locus_tag="PKJ43_00445"
     CDS             complement(6760..>7041)
                     /locus_tag="PKJ43_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNZ71053.1"
                     /translation="KKIDIQKGDLYLLVSDRTLAMILDQAAEKNYEPGSDIGIISYNE
                     TPMKRFVKDGITVLSTDFQLMGKLAAEYVLNPVHTKFVVPTKFILRKSF"
BASE COUNT         1918 a         1491 c         1409 g         2223 t
ORIGIN      
        1 gttagttgat ttttggtttt tatccccgga ggtggttctc cggggttttt attttttgca
       61 attgcttatt tagttttctt ttaaattacc cagaaaaatt gattactatc caacaatccg
      121 aatcgaattc tgtttatgtt tatactcctg taaaccgtca ggataataaa attgtataac
      181 cataaatcaa taaccatgag tttcgaatta ccaaaattga attactcgct tgatgcgctt
      241 gagcctttca tgagtaagca gaccctggag tttcattatg ggaaacacca tcaggcttac
      301 gttaacaacc tgaatggcct tattcccggg accgaatttg aaaatgctac ccttgaagaa
      361 attgtgatga aatcttctgg tggaattttc aacaacgggg cgcaggtgtg gaaccacact
      421 ttctttttta accagttttc aaaagatggc tgcactgaac cacgcgattt gctaaaagat
      481 gctattgaaa aggcttttgg ttcgttcgat gcattcaaag aacaatttac caaagctgca
      541 gtaactctgt ttggaagcgg ttgggcctgg ttggttgtaa accagcaggg agaacttgag
      601 attgttcaaa catcaaatgc agctaacccg atgcgcgacg gaaagaaacc attgttaacc
      661 tgcgacgtgt gggaacatgc ttattacctc gacaagcaga accttagacc taagtatgtc
      721 gaagatttct ggaaaattgt cgattggaaa attatcgctg aacggtatca ataactgatt
      781 ttttctcttt ttgaaaaggt tgttttgctt atttttatca gcggttttta cccatattga
      841 aaaactggag aatttatttt aaacaacatt aaatcaattt gtatgagtaa agagaaagtt
      901 ttagctgtca tgaaaagtgc tggcaagccg ctcaaagctg gggaaattgc agagctggcc
      961 ggactcgaca aaaaagaagt agacaaagca atgaaacagt tgaaagatgc cggagagatt
     1021 gtttctccaa aggtttgttt ctggcagcct aagtaattta caatcggtat aatttgccaa
     1081 ataaaaaatt cccggcaata gcgagtgtat tgtcgggatt ttttctttat tgctcaggta
     1141 aagagccaga tatttttggt gcttattgta ccttcaaaag gttcattact tcatctagct
     1201 tgggcgataa tattatctca gtccgcctgt tgctggcacg gccttcaggg gtatcgttta
     1261 aagctctggg cgaatattca cctttcccgg atgctgtcaa acggatgggc tcaattttat
     1321 gttcatcggt taatattctt acaatcgatg ttgcgcgggc aacactcagg tcccaattgt
     1381 cgcggtaaat ggctgtttta attggtatat tgtctgtgtg tccttcaacc aatatgttga
     1441 tttcaggatt cttcaaaagc acatctgaaa gagcctttag tgcttcttta ccttttgctt
     1501 caactgctgc actacccgac ttaaacagta atttgtcaga catagaaacg tagaccttgc
     1561 cattttttac ttcaaccgat aattcatcgg tttggaaacc cagcagtgca tttctaagta
     1621 cgtcgttcaa ccttttggtt attgaatctt gccgggaaat gacttgtctt aattcatcca
     1681 gcgacatttc tctttcttgc aagagccttt ctttttgggc aagttcttca gttttttgtt
     1741 tcaaagccag gttgaactgt tccgattgag aaaggctttg actcatcaga tcgttatatt
     1801 ttttctcaat ctcggtaacc gattgattta gcgctgcttt ttcgtttgtc aacccagcta
     1861 tttcgttctt taaatcagaa atgtcggttt tcaaccgcac aatttcctga tctctttttt
     1921 caacagtttt gcataaataa tctctttctt cgatgactgt cctgacccgt ttcgacacga
     1981 gaagtttttt ctccggtttt gcttgcccgc attcataaaa tggtttacat gagctgacta
     2041 gcaataacag gacacttgcg gcgaaaagaa tgttctttgt tcccatacgt ttgaattatt
     2101 agattaatac ctgataacgt ttgaagagat gaatttcaat actgacgaaa tcaaatttat
     2161 tgtgacgtca agtaaaacaa aaataattgg agacaagatt gtgcctgcct ccaattattt
     2221 aatattcaag aaatagactc ttataaagag cgtatataag tcttattttt tccagacatg
     2281 ttccatttct tcaagtgtct tcccttttgt ttcgggaacc attttccaaa caaaaatgaa
     2341 cgatagaatg ctcatcagcg cgtagaaccc gtatgtcatt ccaccactga attccatcat
     2401 cgaagggtag gttgatgaaa tgaggtagtt ggcagcccat tgagcggcta cggcaatggc
     2461 tacagctttt cccctgattt tattcgggaa tatttcagaa atgagtaccc agcagattgg
     2521 tccccaagac atcatgaacg aagcagtata cacaatgatg aataccaacg tgctaatgcc
     2581 aattatttcc atgaacgcta atgccgatat ggcaatcata cctacagcca ttcccagcga
     2641 gccaatcatc aacagtggct tacggcccca tttatctaca gttaaaatag cgagtactgt
     2701 aaatgcaacg ttaacgagcc ccattaccac ggtttgcatc attgatgcat ctttggccgc
     2761 gcccatactt tcaaaaatac gtggggcata atacaatgct acgtttattc ctacaaattg
     2821 ttggaatact gaaagaagta taccaataat gataactgtt tttccgtacg aaaatacttt
     2881 tcccgaacta tgttctaccg tatctttaat ttcggcaaga attaattttg ccttttccgg
     2941 cccattaatt ttggtaagta ccttaagcgc ctcatcgtca cgttttgtaa gcgacaagta
     3001 acggggtgtt tcgggaacaa aaaacaaaag gataccgaat aaccctgcag ggatggcttc
     3061 cgataagaac atccagcgcc agcctatatc attgatccat tcgattggtt tcccgttggc
     3121 aatgccccaa ttcacaaaat aaacgaccag cataccaaag atgattgcaa attggttgag
     3181 tgataccaac cggccacgta tttcggcagg tgagatttca ccaatataca taggtacaat
     3241 ggcggatgcc attcctacgc ctacgccacc aagaatgcgg taaaagttaa acatcagaag
     3301 aaggccaatc gatgcttctc cttttggaaa gaaaaggaac tcagggaaac ccgatcctaa
     3361 agcagaaagg aagaacatga cagatgctat gattaacgat ttgcggcggc caaagcgtga
     3421 ggcaaaaagc ccggaaatgg ctccgccgat aatgcaaccg atgagtgcgc ttgaaatggt
     3481 tgctccatgt acaaaagtgc taagcccttg gctggttatg agaaatgcct gaaccgattt
     3541 ctcggcacct gaaataactg cagtgtcgta tccgaacagc aatcctccaa tggttgcaac
     3601 aaaagttaca acaatgataa agaatttgtt ttgtttcatt tggtctgtac ttatgtatta
     3661 atttttgatt ttagttcata taatattgag caaaaataaa aaaacccgat tcaaagtaaa
     3721 ctctgaatcg ggctcaatat ggaaacatga ttttgtaaat cattctgagt tttgatccag
     3781 tcagggatca aatatagcaa attattagaa gtttggccta ttcgcttacc tgctaaattg
     3841 ttttctattc atactttttt agatatacat gtttacaatt gcttcgtaca attcttgctt
     3901 gccactgatt tgttttggtt cgccgtttgc ttttgcatat tcgtaaactt gttcgaaagt
     3961 aagtttgcct tcttcaaatt cttttccttt acctgagtcg taagaagcgt aacgatcggc
     4021 aaccattttc ttgtaaggtg attcttccag aattgcagca gcagtttcaa gagcacgggc
     4081 cattacatcc attgcaccaa tgtgagcgag gaataaatct tcgttatcgg tagagttacg
     4141 acggatttta gcatcgaagt tggtgccacc accttgcatg ccaccagcct gaagaacaac
     4201 catcattgct tgtgtcaatt cgaagatgtc tacagggaat tggtctgtgt cccatccgtt
     4261 ttgaacgtcg ccacggttag cgtcaattgc tcctaacata ccagcatcgg cagcgcattg
     4321 caattcgtgt tcaaaagtgt ggccagccaa tgtagcgtgg ttaacttcta tattcaattt
     4381 gaaatcgttt tccaaaccga attctttcaa gaaaccaata acagtttcag catcaacatc
     4441 atattggtgt ttcattggtt ccattggttt tggttcaatc aagaacacac ctttgaagcc
     4501 ttttgaacga ccgtaatcgc gagccatctt caacatggtg cccatgtgtt gtttttcgcg
     4561 tttcatgttg gtattcaaca agctcatgta accttcacga cctccccaga aaacgtatgc
     4621 tttaccacct aattcgatag tagcgtcgat tgaatttttg atttggagca tagcgcgtgc
     4681 agctgcatca aagttaggat ttgtgcttgc gccattcatg taacgtgcgt ttgagaatac
     4741 gttagcagta ccccacatca aagtgatacc tgtttcagct tgtttttgtt ttgcataagc
     4801 aacaatagct ttcaggttag cttcgtattc ttcgatagaa gaaccttcgc tgattaagtc
     4861 gatatcgtgg aaacaatagt atttgatacc aattttttgc atgaattcga aacctgcatc
     4921 cattttttct tttgcagctt gcaatgcatc agcagcacct acccaagggt gtttttgtgt
     4981 ttgtccacca aatgggtcgc caccatcagc acataatgta tgccaccagg ccattgagaa
     5041 tttaaaccat tcagccatag tttttccata aactactttt tcaggattgt agtaacggaa
     5101 tgctaatggg tttttacttt cttttccttc aaatttaatt tgtcccactg atgggaaata
     5161 aacttttgca ctcatgatca aatttattaa ttgtgaatta tttatttttt taattatcaa
     5221 gccggatttc gggatgatat attcatttaa atgtgtttac ctaacagagc tttccagctt
     5281 tcataagcag cttcgtattc tgctttctta gcaagatttg gctctattgt ttcaagtttc
     5341 ttcaagctac caaatgcttc agcaaacgat ttgtaatatc ctgatccaac tgctgcacca
     5401 cgggctgcac ctaccgaccc gtcggtattg tacaattcaa tggttgcacc gctgattccg
     5461 gcaagagttt cacggaaaat tggactgagg aacatattgg ctttcccggc gcggataact
     5521 tttgcgtcga taccaatctg cttcatgatt tccattccat agttgaaaga gaacacgatg
     5581 ccttcttgag ctgccctgaa taggtgagcc tgagagtggc tgttgaagct gattccgcta
     5641 atttgcgacc caatgttttt gttccgcaat acgcgttcgg ttccgtttcc aaaaggcaaa
     5701 atgcttactc cgtcggaacc aatggctact ttcgatgcca attcgttcat ttcggcgtag
     5761 ttataagcgt tgttgccaac attcttgttc atccacgagt tgagaatacc tgttccgtta
     5821 atgcaaagca gtacgccaag acggttgagg tctgtagtgt agtttacatg ggcaaaggta
     5881 tttacccttg aatagggatc atactttact tcaccgctaa caccgtacac aacgcccgat
     5941 gttccggctg tagtagctat ttcacccgga ttcagtacat tgagtgataa ggcattattc
     6001 ggctggtcgc cgccgcggta ggttacttta gtccctgctg ccaacccgag ttctttggca
     6061 acttcggctg ttacttcgcc ttgttcagag aaggtgggta ctatttcagg aatcatagaa
     6121 ctgttgaacc cgtaatgatc catgatcatt ttcgagactt catttttctg aaagtcccag
     6181 aacatacctt ccgatagccc cgatatggta gttcgggcct ggccggttaa gcgtaacgca
     6241 atgtagtcgc cgggtaacat tatcttgtcg atttgtgcat acacgttggg ttcgttttct
     6301 ttaacccaca taagtttcga cgcagtaaaa ttgccgggtg agttcagcaa gcttccaaga
     6361 caacgttctc cgccaagggt ttcgaaggcc ttgtcgccaa taactgcggc acggctgtcg
     6421 caccaaataa ttgacggacg aagcacattg ttgtttttgt cgacaacaac taacccgtgc
     6481 atttggtacg atatcccgat ggcttcaatc tctttggcgt ctatttccga cgatgccatt
     6541 accgattggg tagccagttt caggttgtcc caccataagt cggggtgttg ttcggcccag
     6601 cctgcttttt gggctgtaat cttcatttct tgtttcggga aaaagtcgga tgctacgcat
     6661 tctccggttt ctccatttat cagtgaagct tttaccgatg agcttccaac atcatagcct
     6721 aaaaagtaca tctctcttcc ctttttttaa ggttattatc taaaatgatt ttcgcaaaat
     6781 aaatttagtc ggtacaacaa attttgtgtg tactgggtta agcacatatt cggccgcaag
     6841 ttttcccata agctgaaagt cggtcgaaag aacagtaatc ccgtctttta caaatcgttt
     6901 cataggggtt tcgttgtacg atatgatccc aatatcgctt ccaggctcat aatttttttc
     6961 tgccgcctgg tcgagtatca tggccaaggt acggtcgctt accaataagt acaggtctcc
     7021 tttttgaatg tctatttttt t
//
LOCUS       DAOPUW010000012         6998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_10740_length_6998_cov_3.402276, whole genome shotgun sequence.
ACCESSION   DAOPUW010000012 DAOPUW010000000
VERSION     DAOPUW010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 6998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6998
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10740_length_6998_cov_3.402276"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PKJ43_00450"
     CDS             <1..794
                     /locus_tag="PKJ43_00450"
                     /inference="COORDINATES: protein motif:HMM:NF019849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HNZ71054.1"
                     /translation="RLKYGFVSLTEIAANKQPDSSLWLLIAENGNYMIGNFDGKKFEN
                     LTSFNKFDYGEMVGGSLIFKDLKNNRHLLFSEIKSEQQPDLPANGQLTFPTEIILKET
                     NTGLELHHKPIEEIKQLYKKSFVTDNKKIYPGINNNPLQSIKGDCFHMKGTIDLKNCD
                     MFGIIIRGNRENEGINLSYNTSRKMFEIEGSRFTYSPKNNKVDIELLIDRSSVEVFIE
                     GGRYVISTPMIPEPKNLKYNLYSTGGEVFVEHFEVHELKSAWQNK"
     gene            complement(800..1771)
                     /locus_tag="PKJ43_00455"
     CDS             complement(800..1771)
                     /locus_tag="PKJ43_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ71055.1"
                     /translation="MVRKMLLPVSVFLFVGFMLTMIQLKVEMPMLLLERFFEGGGWIE
                     IVAVSFFGAFVVHKMEDVRESGRWRKIIWTIYSVWFFSQLFLGIFASEIFLLTGKLHL
                     PIPAMMIAGPIFRWQKSIMTLLFLSTIVLTGPAWCSQLCYFGAIDNAFSKGKPVRGPL
                     KNKMVYKHGVLLLVILGAILARLFNLSMTWAAVAGILFGLAGFVVMILFSRRQRRMVH
                     CSAFCPIGTLVNYLRFVNPFCIVIDNSCDSCMACSAKCNYDALNIADIRNKKPGFTCT
                     LCGDCISACKKNSLQYKFFSMRPAKARNLYLFITVSVYMIFLAMGRI"
     gene            complement(1774..2355)
                     /locus_tag="PKJ43_00460"
     CDS             complement(1774..2355)
                     /locus_tag="PKJ43_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71056.1"
                     /translation="MEFYQSITKLYDHIFPLNMGQVNFVGSENRVVAFSQGDMLAINQ
                     LYIDQRFAVITCFGNTLVHLNTYDEVSDFLQKTYALLQKKGKLLLQLLNYEAILTKKP
                     EKIPTIENERICFERKYYYRPDGKIDFEGILLDKETGLITRNVVSLLPVTQKQLFLIL
                     NDAGFANLQFYGGFDRSQVDENKLPLVVSAQKI"
     gene            2489..3067
                     /gene="nrfH"
                     /locus_tag="PKJ43_00465"
     CDS             2489..3067
                     /gene="nrfH"
                     /locus_tag="PKJ43_00465"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HNZ71057.1"
                     /translation="MKLFKIFIPPDRWKVPVILLLGIFVGLGLFTIYLSKAPTYLSDD
                     PKTCMNCHIMAPQYSSWSHSSHRQYTNCMDCHVPHNNLVNKYYFKAKDGLRHSSMFAL
                     RLEPQVIFIKDKGKEVVHNNCIRCHQHLLTDERMKQQSEMYMSLREGRSCVECHQHTP
                     HGRVNSLSSVPYARVPILESPVPEWMKKSQKK"
     gene            3104..4618
                     /gene="nrfA"
                     /locus_tag="PKJ43_00470"
     CDS             3104..4618
                     /gene="nrfA"
                     /locus_tag="PKJ43_00470"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase"
                     /protein_id="HNZ71058.1"
                     /translation="MKSITQRIKEKPVIGWLLFFATVLVVFLLGLLASSIIERRAETL
                     FVYTPKVKHTQFEPRNEVWGKNFPREYQSWLRTADTTFKSKYNGSATIDMLEVDPRLV
                     VLWAGYAFSKDYKQSRGHYHAIDDIRTVLRTGAPMNGEKSPQPTTCWTCKSPDVPRLM
                     NEMGVAEFYKGTWEKLGAQVVNPIGCGDCHDAKTMNLRISRPALIEAFERTGKDVAKA
                     SYQEMRSLVCAQCHVEYYFNKKKVEGAQYLTFPWDKGMNVDLIEKYYDEIEFSDWQHA
                     LSKAPMLKAQHPDYELYLTGTHASRGVSCADCHMPYMSEGGQKFTDHHIQSPLNNISN
                     SCQVCHREETDQLVKDVYDRQDKIIENRDKLEELLVHAHIEAQKAWELGANEEQMKPA
                     LTDIRHAQWRWDFSAAGHGSSFHAPVEVGRIISTGIVKAQEARLKLSRILAEKGFTKE
                     IPYPDIATKAKAQKFIGLDMEKLNAEKQQFMKDVIPQWIDEALVREKTYPTSEL"
     gene            complement(4709..5374)
                     /locus_tag="PKJ43_00475"
     CDS             complement(4709..5374)
                     /locus_tag="PKJ43_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776538.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNZ71059.1"
                     /translation="MNEILSKLFEAIEFGKADKTSPYPPALKGLDGAKEYTISALDDG
                     ITPKEILEEALIPAMGCVGKKFSENKIFVPQMLMSAKAMSASMIQLKPFFASGELKTK
                     GTFIIGTVKGDLHDIGKNLVSMIVEGGGWTLVDLGVDVSSDRFIDAIRKNPGAIVGLS
                     ALLTTTMGSMAEIVAKIKSELPGTKVLVGGAPVNMEFCQTIGADYYAPNPQLAVDYLN
                     SVA"
     gene            5486..6349
                     /locus_tag="PKJ43_00480"
     CDS             5486..6349
                     /locus_tag="PKJ43_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNZ71060.1"
                     /translation="MVKGKIRAQDEEHFRIGIFQDNLEKSTWHYHNHYEISFITEGTG
                     KRIVADSMESFHPGDLVFIGQNLPHVWIADKARFALSNRNLEMVYLQFSTTILFPQLL
                     SMAEFANVRKALEMSERGIQIVGDTLNKVSEIMLQLPYLNGIEQLLNFYRLMDIIGKS
                     TSNIALASEEYLRKKFNTSNKRIEAIHDYFMNNYRNEVNLEELSQLVNMAKGSLCRFF
                     KENSGITLWEYLNHIKIEFACKMLMNNELSIAQVCFDSGFNNLNHFNRQFRKITGITP
                     SSYRRKFSELS"
     gene            complement(6366..6689)
                     /locus_tag="PKJ43_00485"
     CDS             complement(6366..6689)
                     /locus_tag="PKJ43_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNZ71061.1"
                     /translation="MNMTSNNDQRITDIIEMLREVYDPEIPVNIYDLGLVYSIDVDES
                     NMAHVVMTLTAPGCPVADMIVQNVYENVCSVEGVDDAFVDLTFDPPWDKSMMSEEARL
                     ELGFF"
     gene            complement(6695..>6998)
                     /locus_tag="PKJ43_00490"
     CDS             complement(6695..>6998)
                     /locus_tag="PKJ43_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HNZ71062.1"
                     /translation="YKVDQNLIRGCQSQVWLNAECRDGVVYYQGESDALIVKGLVALL
                     LRVVNEQPAAEIMNADFHFIDSIGLKEHLSPTRSNGLLAMVKQIKLYAVAFNKMNS"
BASE COUNT         2282 a         1567 c         1357 g         1792 t
ORIGIN      
        1 tcagattaaa atacggattt gtttcgctca ccgaaattgc cgcaaacaaa caacctgact
       61 catcgctttg gctgctgatt gccgaaaatg gaaactacat gatcggtaat tttgatggga
      121 aaaagttcga gaacctcact tcgtttaata aattcgatta cggagaaatg gtgggcggat
      181 cgttgatttt caaagactta aaaaataacc ggcatctcct tttctcagag ataaaaagcg
      241 agcagcagcc cgatcttccg gccaacggcc aactaacttt tccaactgaa atcatcctga
      301 aagaaaccaa taccggtctt gagctgcacc ataaacccat tgaggaaata aagcagctct
      361 acaaaaaaag tttcgttacc gataataaaa aaatatatcc gggaataaac aacaacccgt
      421 tgcagagcat taaaggcgat tgcttccaca tgaaagggac aatcgactta aaaaattgtg
      481 atatgttcgg catcattatc cggggtaaca gagaaaacga agggattaat ctttcctata
      541 atacctcgag aaaaatgttt gaaattgaag gtagccgttt tacctacagc cctaaaaaca
      601 acaaagttga catcgagttg ctgatcgacc gctcgtctgt cgaggtattc atcgagggcg
      661 gaagatatgt gattagcaca ccaatgattc ccgagcctaa aaacctgaaa tacaatttat
      721 attctaccgg aggagaagtc tttgtcgaac acttcgaagt acacgaacta aaatcggcat
      781 ggcaaaataa atagcgggat cagattcgcc ccatggccaa aaaaatcatg taaaccgaca
      841 cggttataaa taaatacaag ttgcgggcct tggctggtct catgctgaaa aatttgtact
      901 gaagggaatt cttcttacaa gccgaaatgc agtcgccaca aagagtacag gtaaacccgg
      961 gttttttgtt tcttatgtca gcaatattta gtgcatcgta attgcatttt gcagaacaag
     1021 ccatgcaaga atcacagctg ttgtcgatta ctatacagaa gggattaaca aaccgcaagt
     1081 aattcaccag cgttccaata ggacaaaatg ccgaacaatg aaccatacgt cgctgccgcc
     1141 gcgaaaaaag gatcataacc acaaagcctg ccaagccaaa caaaattcct gcaacagcag
     1201 cccaagtcat tgataagttg aaaaggcgcg caagtattgc cccaagaatt actaacaata
     1261 aaactccgtg tttataaacc atcttgtttt tcaacgggcc cctgacaggt tttcctttag
     1321 aaaaagcatt atcgattgcc ccaaaataac aaagctggct acaccaggcc gggcccgtca
     1381 gtacaattgt actcagaaac agaagggtca ttatcgattt ctgccagcga aaaattgggc
     1441 cagcaatcat cattgcaggg attggcaaat gaagtttccc ggtaagcagg aaaatttctg
     1501 aagcaaaaat cccaagaaaa agttgggaaa aaaaccaaac ggaatagatt gtccagataa
     1561 ttttcctcca ccgccctgat tctcttacat cttccatctt atgaaccaca aatgcgccaa
     1621 aaaaggatac agccacaatt tctatccacc ctcccccttc aaaaaagcgt tcgagcagaa
     1681 gcatgggcat ttcaaccttt aattgaatca ttgtcagcat gaaaccaaca aataaaaaaa
     1741 ccgagaccgg taaaagcatt tttcttacca tagctaaatt ttttgtgccg aaacgaccaa
     1801 gggaagctta ttttcatcga cctgtgaacg gtcgaaacca ccataaaact ggagatttgc
     1861 aaagcctgca tcattcagaa tgagaaacaa ttgcttctgg gttactggta aaagtgagac
     1921 aacattacgg gtaatcagcc ctgtttcttt gtccaaaaga atcccttcaa aatcaatttt
     1981 cccgtcgggc cggtagtaat actttcgttc gaaacagata cgttcattct caatcgttgg
     2041 aatcttttca ggtttttttg tcaaaatcgc ctcataattc aacagttgaa gaagaagctt
     2101 tccttttttc tgaagcaacg catacgtttt ttgcaaaaag tcagaaacct cgtcgtatgt
     2161 atttaaatgg accaaagtat tcccaaaaca ggtaataacc gcaaagcgct gatcaatata
     2221 cagttggtta atagcaagca tatcgccctg agagaaagca accacacgat tttcggaacc
     2281 cacaaaattg acctgcccca tattcaacgg gaaaatatga tcgtaaagct ttgtaatcga
     2341 ttgataaaac tccattctgt ccagtaatta agctacaaaa ctaatgaact tgcctaaata
     2401 gatttgtaac aattgaaaca acattcataa aagcccgtat tgatatttgc aaaagaatat
     2461 tataaattta aaacgtaaaa gttgcacgat gaaactcttt aaaattttca tacctcccga
     2521 ccgatggaaa gttccggtaa tactccttct gggaatattt gtcggtcttg gactgtttac
     2581 aatatatctt tctaaagctc cgacttatct ttcagatgac ccaaaaacct gcatgaactg
     2641 tcacataatg gcgccacaat attcctcgtg gagtcacagt agccacagac aatataccaa
     2701 ctgcatggat tgtcacgtac ctcataataa tttggttaac aaatattatt tcaaggcaaa
     2761 agacggactg cgccactcgt cgatgtttgc ccttcgcctt gagccgcaag ttattttcat
     2821 taaagacaaa ggaaaagaag ttgtacacaa caattgcatt cgctgccacc agcacttgct
     2881 caccgacgag cggatgaagc aacaatcgga aatgtatatg agccttcgcg aaggacgctc
     2941 ttgtgttgaa tgccatcaac ataccccaca tggcagggtc aacagcctct cgagcgtgcc
     3001 ctatgcgcgg gttccaattc tggaaagccc cgtacctgaa tggatgaaaa aaagtcaaaa
     3061 aaaataaata agcaacaata aaacctttat aaatacgaac cagatgaaat caataacaca
     3121 acgaatcaaa gaaaagccgg taattgggtg gctactgttt tttgccacag tgctggttgt
     3181 atttctattg ggtcttctgg cctcatcaat cattgagcga agggccgaaa cattgttcgt
     3241 gtatactccg aaagtaaaac acacgcagtt tgaaccaagg aacgaagtct ggggcaaaaa
     3301 tttcccccgc gaataccaat cgtggctccg aacagccgat acaacattca agagcaaata
     3361 caacggaagt gccaccatcg acatgctcga ggttgacccg cgcctggttg tactgtgggc
     3421 tggctacgct ttttcaaaag actataaaca atcgagagga cattaccatg ccatagatga
     3481 tatcagaaca gttttacgta cgggtgcccc gatgaatggt gaaaaaagcc cgcaaccaac
     3541 cacttgctgg acctgcaaaa gccccgacgt tccgcgcctc atgaacgaaa tgggtgttgc
     3601 cgaattttat aaaggaacat gggaaaagct tggtgctcaa gttgtgaacc ccattggttg
     3661 tggcgactgc cacgatgcta aaaccatgaa cctgcgaatc tctcgtccag cccttatcga
     3721 agcattcgaa cgcacgggaa aagatgtggc aaaagcttcg taccaggaaa tgcgctcgct
     3781 ggtttgtgct caatgccatg ttgaatatta tttcaacaaa aagaaagtag aaggggcaca
     3841 atatctgact tttccatggg ataaggggat gaatgtcgac ctgattgaaa aatattatga
     3901 cgaaattgaa ttttccgatt ggcaacatgc cctgagcaaa gcacccatgc taaaagccca
     3961 gcatccagac tatgaacttt acctgaccgg gacccatgcc agccggggtg tttcgtgtgc
     4021 cgattgccac atgccttaca tgagcgaagg cgggcaaaaa ttcaccgacc accatataca
     4081 aagcccgctc aacaatatct cgaattcctg ccaggtttgt caccgcgaag aaaccgacca
     4141 actggtgaaa gatgtttacg accgacaaga taaaataatt gaaaaccgcg acaaacttga
     4201 agagctcctg gtacatgccc atattgaagc ccaaaaagca tgggaacttg gagcaaacga
     4261 ggagcaaatg aaacctgccc tcaccgacat aaggcatgca caatggcggt gggatttttc
     4321 ggctgccggt catggctctt catttcatgc tccggttgaa gtaggccgga taatatcaac
     4381 aggaattgta aaagcacaag aagcccggct aaaattatcg cgcattttgg ccgaaaaagg
     4441 ctttaccaaa gaaattcctt accccgacat tgccacaaag gcaaaagcac aaaagtttat
     4501 cggattggac atggaaaaac tgaatgccga aaaacaacaa ttcatgaaag atgtaatccc
     4561 tcaatggata gacgaagcat tagtaaggga aaagacatac cctacaagcg aactgtaaga
     4621 atataatcga actcaggtta ataataacgg acagttttaa tcaataaaac cgtccgtttt
     4681 ctatgaagaa ccaaaatgat tgatctttct atgctacgct attgaggtag tcaacagcta
     4741 gttgtggatt tggagcatag taatcggcac caatggtttg acaaaattcc atattaacag
     4801 gagcccctcc aaccaaaact ttggtgccgg gaagttcact tttaattttt gcgacaattt
     4861 ctgccatact gcccattgtt gtggtaagca acgccgaaag gccaacaatt gcgccaggat
     4921 ttttacgaat ggcatcgata aaacgatcag aacttacatc caccccaagg tctacaaggg
     4981 tccatccgcc tccttccaca atcatcgaaa ccaaattctt tccaatatcg tgaaggtcgc
     5041 cttttaccgt gccaataata aatgtaccct ttgtctttaa ttcgcccgat gcaaaaaaag
     5101 gttttagttg aatcattgac gcactcatgg cttttgccga cataagcatt tggggaacaa
     5161 aaattttatt ctctgaaaac ttttttccta cacagcccat agccgggatc aatgcttctt
     5221 caaggatttc cttcggtgta attccatcat caagggcact tatcgtgtat tctttggcgc
     5281 catcgagccc ttttaatgct ggtggatagg gtgaagtttt gtcagctttt ccaaattcta
     5341 tcgcttcaaa aagcttactg agaatctcgt tcataagtat tacatttaac gcctaaaaat
     5401 aggagattag aataactcag gctacactaa tattgactac ctttagaaca atattaacat
     5461 attttctctt ttttgtttat attatatggt aaaaggaaag attcgtgcac aagacgaaga
     5521 acattttcgt ataggaattt ttcaagataa tctggaaaaa agcacttggc attatcacaa
     5581 tcactacgag atcagcttta tcacagaagg tacaggcaag cgtattgttg ccgattcaat
     5641 ggaaagtttc catccgggag accttgtgtt tattggccaa aatttgcccc acgtctggat
     5701 tgccgataaa gcccgttttg ccctttcgaa ccggaacctc gagatggttt acctgcaatt
     5761 ttcaacaaca atattgtttc ctcaattgtt gtcgatggct gagtttgcca atgttcgtaa
     5821 agcactggaa atgtctgaaa ggggaattca aattgtaggc gacaccttaa acaaagtgag
     5881 cgaaataatg cttcagttgc catatctcaa tggcattgaa caacttctga atttctacag
     5941 attgatggat attattggca aaagcacctc aaacatagcc cttgccagcg aagaatattt
     6001 gaggaaaaag tttaatacca gcaacaaacg aattgaagca atccatgatt atttcatgaa
     6061 caattatcgc aatgaggtca atcttgaaga gttatcccaa ctggtaaaca tggcgaaagg
     6121 atcgttatgc cgcttcttca aagaaaactc gggcattacc ctttgggaat atctcaacca
     6181 catcaaaatt gaatttgcat gcaaaatgct tatgaacaat gaattaagca tcgcacaagt
     6241 atgtttcgac agtggattta acaaccttaa ccatttcaac aggcagttcc gcaaaatcac
     6301 agggattact ccttcgtcgt accggagaaa attctctgaa ctgtcatagt aaatcaagaa
     6361 aagatttaga agaaacccaa ttcaagacgg gcttcttcgc tcatcatcga tttgtcccag
     6421 ggtggatcaa aagtcaaatc aacaaacgca tcgtcgacgc cttcaaccga gcacacattt
     6481 tcatatacat tctgaacaat catatcggca accgggcagc caggagccgt aagggtcatc
     6541 accacatgtg ccatgttact ctcatcgaca tcgatactgt agactaaccc caaatcgtat
     6601 atgtttacag gtatttcagg atcgtacacc tcacggagca tctcgataat atcggtaatt
     6661 cgctggtcgt tattgcttgt catattcatt tcggttaact attcatttta ttgaaagcaa
     6721 cagcataaag ctttatttgt tttaccatag cgagcaatcc gttcgaccgc gtaggcgaaa
     6781 ggtgttcttt cagcccaata ctatcgataa aatggaaatc ggcattcatt atttctgcgg
     6841 ctggctgctc attcaccacg cgtaacaaca aagccactaa tcctttaaca atcaatgcat
     6901 cgctttcacc ctgataatac actactccat cacggcactc agcattaagc caaacttgcg
     6961 actggcaacc ccgaatcagg ttttggtcaa ctttatat
//
LOCUS       DAOPUW010000013         6928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_10885_length_6928_cov_3.902517, whole genome shotgun sequence.
ACCESSION   DAOPUW010000013 DAOPUW010000000
VERSION     DAOPUW010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 6928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6928
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10885_length_6928_cov_3.902517"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /gene="metK"
                     /locus_tag="PKJ43_00495"
     CDS             <1..927
                     /gene="metK"
                     /locus_tag="PKJ43_00495"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNZ71063.1"
                     /translation="MFGYACKETDNYMPLSLELSHKLLLELAAIRREGKVMTYLRPDS
                     KSQVTIEYNDNGDPVRVDTIVVSTQHDDFIHPVNNSVEAQLKADEEMIAKIKADVVSI
                     LIPRVLATLPERVKALFNDKIIYHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAH
                     GGGAFSGKDPSKVDRSAAYACRHIAKNLVAAGVADEILVQVAYAIGVAKPMNIFVNTY
                     GKSNVKCSDSVIAQKVAEIFDMRPKAIEQRLKLRNPIYEETAAYGHMGREPKTVVKKF
                     NSSYFENIEVEVELFTWEKLDYVDKVKAAFGL"
     gene            complement(991..2880)
                     /locus_tag="PKJ43_00500"
     CDS             complement(991..2880)
                     /locus_tag="PKJ43_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNZ71064.1"
                     /translation="MEINNLSVERTFDLLDLYNTEYSSKKVALGGKNANGWYTYSAKE
                     YVDYVNNLSYGLLSIGINRGDKIATVTVNRPEWNFIDLAIAQIGAVHVPIYPTISIEE
                     YKYILEHCEARVVFVGDKKLYSKICDLVSETSMLEKIYSFDELESVENWKKLVELGEQ
                     FENKPLLDEIKASISSNDLATIIYTSGTTGVPKGVMLSHENLCSNFKAHSKHHPLGKE
                     STAISFLPLCHVYERSMNYHFQYKGLAIYYVGNLGQIMPALKEIKPDIFNTVPRLLER
                     IYDGLIDKGKELKGIKKTLFFWAVKLGKKFDYGKPFSIFFNIKRKIADKLIYIKWREA
                     LGGNVKYVVSGGAALQPRIARVLGIANIQAIEGYGLTETSPVIAVSNITTNEIRIGTV
                     GPILAGVEVKFAEDGEILCKGPNVMMGYYKDEERTQMAVDQDGFFHTGDIGILVEGKY
                     LKITDRKKEIFKLSGGKYIAPQMIENRLKESMFIENAMVIGENEKFASALIVPSFSHI
                     DKWAAGKGLAFSSHEEMINHPEVLSTLQREVSEINKTLGQVEEIKRFRLVPDSWSPES
                     GEMSPTLKLKRNYIAKKYADIIEDIFATSRDEEPNGLKKLKIPKINLSLSELINKLRN
                     GNIFV"
     gene            complement(3150..5768)
                     /gene="alaS"
                     /locus_tag="PKJ43_00505"
     CDS             complement(3150..5768)
                     /gene="alaS"
                     /locus_tag="PKJ43_00505"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNZ71065.1"
                     /translation="MTSQEIRKAFLDFFKSKEHQIVSSAPMVVKNDPTLMFTNAGMNQ
                     FKDIFLGNEPAKYRRVADTQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     KEAIGWAWEFLVDKMGISPDRLYATVFEGNEEDGVARDNEAFEYWKQFLPEDRILLGN
                     KKDNFWEMGDTGPCGPCSEIHVDIRQDSERALIAGKDLVNNDHPQVIEIWNNVFIQYN
                     RKANGKLEELPAKHVDTGMGFERLCMVIQGKQSNYDTDVFQTIITEIGKLAGKKYGID
                     AKQDIAMRVIADHLRAISFAIADGQLPSNNKAGYVIRRILRRAVRYGYTFLNFREAFI
                     YRLVSVLKDQMGNAFPELVAQENLIAKVIKEEEESFLKTLETGLRLLDGMIQKVKADG
                     KNEVDGKDAFTLYDTFGFPLDLTELILKENNLSVNIEQFNAEMEQQKSRSRNAAAQEA
                     GDWIEIRNSDSVEFLGYDFETTEVRITRYRQVKTAKKTLFHLVFDKTPFYAESGGQVG
                     DSGHIEADNEIIAIIDTQKENNLIIHITEKLPSDPSTTFIAIVPHETRMRTANNHSAT
                     HLLDHALRTVLGSHVEQKGSLVTPNYLRFDFSHFQKVTDEELLTVQRLVNAAIRENIS
                     CNEFRSIPLKEANEMGAIALFGEKYGEEVRVIKFGDSIEFCGGIHVKATGQIGMFQII
                     SESAISAGVRRIEAITGQKVEEYLENATSTLKQLKSLLNNPSDIPQAIQDLHDKNSAL
                     SKQIEEVERKEAMKIKDSLKRKAQLINGITIISETLTLENAALIKDICFQLRNEIPNL
                     FMVVGASIAGKANLSIIISDNLVAERKLHAGNIVREAAKAIQGGGGGQPFYASAGGNN
                     PEGLKQAYEIALSSIK"
     gene            6004..6897
                     /locus_tag="PKJ43_00510"
     CDS             6004..6897
                     /locus_tag="PKJ43_00510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNZ71066.1"
                     /translation="MLILGFLVFFVIVSIAFSTLFVSHFETPEVKSLRKENQQLLSQY
                     NFLNSKLTQLETVLEELQVRDDNIYRVVLGKNPIPEDVRKAGFGGDNRYASLEQLSDA
                     EIVLNTSKKIDEISKQAYIQAKSYEEVLNIALKKDREMACTPAIMPIANKDLDHTSSG
                     WGMRIHPIYKVKMFHWGIDFVAPKGTKVYSTGDGIVKETPSLRDGHGKHVVIDHGFGY
                     QTLYAHLSAFNVKPGDKVKRGQVIGFIGSTGTSTAPHLHYEVNKDGRNVDPKYYFFKD
                     LTPEQFAQLVARSSNNGQSFD"
BASE COUNT         1921 a         1383 c         1428 g         2196 t
ORIGIN      
        1 atgtttggat acgcatgtaa ggaaaccgac aactatatgc cactatctct tgagttgtcg
       61 cataaattac ttctcgaact ggcagcaatt cgccgcgaag gaaaggtaat gacttatttg
      121 cgtcccgact cgaaatcgca ggttaccatt gaatacaacg ataatggaga ccctgttcgt
      181 gtagatacca ttgtggtttc tactcagcac gacgacttta tccatcctgt taataattca
      241 gttgaggctc agttgaaagc tgatgaagag atgattgcca aaatcaaagc cgatgttgtt
      301 tcgattttga tccccagggt attggctaca ttgcccgaac gcgttaaagc gttgttcaac
      361 gataaaatta tctaccatgt aaatccaact ggtaaatttg ttattggagg tcctcacgga
      421 gatacaggtc ttaccggtcg taaaattatt gttgatacct acggaggaaa aggagcccac
      481 gggggcggag cattctcagg taaagaccct tcgaaagttg accgttcagc ggcttatgct
      541 tgcaggcaca ttgcaaaaaa ccttgtggca gctggtgttg ctgacgaaat tcttgttcag
      601 gttgcttatg ctattggcgt tgcaaagcca atgaatattt tcgttaacac ctatgggaaa
      661 tcgaatgtga aatgttccga tagcgttatt gcacagaaag ttgccgagat cttcgacatg
      721 aggccaaaag ctattgaaca acggttgaaa cttagaaacc cgatttatga agaaacagcg
      781 gcttatgggc acatgggacg cgaacctaaa acagttgtga aaaagttcaa ctcttcatat
      841 ttcgaaaata tagaggttga agtagaactg ttcacttggg aaaaacttga ctatgttgac
      901 aaagtaaaag cagcatttgg tttgtaatac agttcattat ataacaaaaa aagagaagca
      961 cttgcttctc tttttttgtt agtgtgatat ttaaacgaat atatttccgt ttcgaagttt
     1021 gttgataagc tcgcttaaac tgaggttaat tttcgggatt ttcagtttct ttagtccgtt
     1081 aggttcttcg tcacgcgaag tggcaaaaat atcttcaatg atatcggcat actttttagc
     1141 gatgtagttt cgcttcagtt taagagtagg cgacatctca cccgattcgg gcgaccaact
     1201 gtcgggcact aatctaaaac gtttgatctc ttcgacttga ccaagggttt tgttaatttc
     1261 tgaaacttca cgctgtaatg ttgaaagaac ctcagggtgg ttgatcattt cttcgtgaga
     1321 agagaaggcc aggcccttac ctgcagccca tttatcaatg tgactgaacg aaggaacaat
     1381 tagcgctgaa gcaaacttct cgttttcgcc aattaccatc gcattttcaa taaacatcga
     1441 ttcctttaga cggttttcaa tcatttgagg agcaatatat tttcctcctg aaagtttgaa
     1501 aatctctttt ttccggtctg taattttcag gtatttacct tctacaagaa ttccaatgtc
     1561 tcctgtgtgg aaaaatccgt cctggtcaac agccatttgt gtgcgttctt catctttgta
     1621 atagcccatc ataacatttg gtcctttgca aagaatttct ccatcctcgg caaatttcac
     1681 ttcaacaccg gcaagtatgg gaccaactgt gccaatgcga atttcattag tagtaatgtt
     1741 gctgaccgcg attacaggtg aggtttctgt aaggccatat ccttcaatgg cttgaatgtt
     1801 ggcgatgcca agtactctgg caattcgtgg ctgcaaggct gctccgcccg agacaacata
     1861 tttcacattc ccgcccaacg cttctctcca ttttatatag atcagtttgt cagcaatttt
     1921 gcgtttaata ttgaaaaata ttgagaaagg tttaccatag tcgaactttt ttccaagttt
     1981 gacagcccag aaaaataacg tctttttgat gcctttcaac tcttttcctt tatcgatcag
     2041 tccgtcgtaa attctttcca ataaacgggg cacggtatta aaaatgtcag gttttatttc
     2101 ttttagcgca ggcattatct gtccgaggtt tccaacataa taaatggcaa gtcccttata
     2161 ttgaaaatga tagttcatgc tgcgttcgta aacatgacag agtgggagaa aacttatggc
     2221 tgtagattct ttgcctaatg ggtggtgttt tgagtgggct ttaaagttgg agcataaatt
     2281 ctcgtgcgac aacattacac ctttgggcac acctgtggtt cctgaggtat atattatggt
     2341 tgccaaatca ttgctcgaga tagatgcttt aatttcatcc agtaaaggtt tgttttcaaa
     2401 ttgttcgcct aattcaacca attttttcca gttttcaacc gactccagtt cgtcgaacga
     2461 ataaatcttt tccagcatgc tggtctcact gacaagatcg cagattttgc tatacagctt
     2521 tttatctcct acgaacacaa ctctagcttc gcaatgctca aggatgtatt tatattcttc
     2581 gatactaatg gttggatata tgggtacatg cactgctcca atttgggcta tcgccagatc
     2641 gataaaattc cattcggggc ggtttacagt tactgtggca attttatcgc ccctgttaat
     2701 cccaatggaa agcaatccat aactcaggtt gttgacatag tcgacatatt ctttggctga
     2761 ataggtgtac caaccattgg cattttttcc acccaaggct actttcttcg aagagtattc
     2821 ggtattatat aagtcgagaa gatcgaacgt ccgttcgacc gacaggttat taatttccat
     2881 aaataaaaaa aatgttttcc agatttccaa tcgtctaaag tataataatc caataaacca
     2941 tgaagcttca tagggcttat atcgataaat aaataatgat tattcattat aagaacttga
     3001 gaaacagaaa tatattttta ttctggttag tagcgtgatt ttgaatttta aagaataaaa
     3061 gttgaaaaag ccatggattt ccatggcttt ttcaactttt atcaagtatt atatcacttg
     3121 gttaatatac caagttagat aatgagtctt tattttatcg aagacaaggc aatttcatag
     3181 gcttgtttga gcccttccgg gttgttccct ccagccgaag catagaacgg ttgcccgcca
     3241 cctccgcctt gaattgcttt tgctgcttcg cgaactatgt tcccggcatg taactttctt
     3301 tcagccacta aattgtccga aataataatc gacagattgg cttttcccgc aattgacgct
     3361 ccaacgacca taaacaagtt aggaatttcg ttgcgcaact ggaaacagat gtctttaatg
     3421 agagcggcgt tttccagtgt tagcgtttcg ctaataatag taatgccgtt tataagctga
     3481 gcctttcttt tcaatgaatc tttaatcttc atggcttctt ttcgctcaac ttcttcaatt
     3541 tgcttggaaa gagcagaatt cttatcgtga agatcctgaa ttgcttgcgg gatatcggat
     3601 gggttgttaa gcagcgattt cagttgcttt aatgttgagg ttgcattttc cagatactct
     3661 tcaacttttt gccctgtaat ggcttcaatt cgccgaacac cggcagaaat tgcactttct
     3721 gaaataattt gaaacattcc aatttgtccg gttgctttta cgtgtatgcc accgcagaat
     3781 tcaatagagt cgccaaattt gataacgcga acttcttctc cgtatttttc cccaaagagg
     3841 gcaatagctc ccatctcgtt ggcttctttg agcggaatgg aacgaaattc attgcaggaa
     3901 atattttccc tgatcgccgc attaaccaaa cgttgtaccg tcagcaactc ttcgtcggtt
     3961 actttttgga aatggctgaa gtcgaaacgc aagtaattgg gtgtaaccag cgaacctttt
     4021 tgttcgacat gtgaacccaa tactgtacgc aacgcgtggt cgagtaagtg tgtggctgag
     4081 tggttgttgg cagtgcgcat gcgtgtttcg tggggcacaa tagctatgaa agttgttgat
     4141 gggtcagagg gaagtttttc ggtgatatga ataatcaggt tgttttcttt ttgagtgtcg
     4201 ataatggcaa ttatttcatt atcggcttca atatgtcccg aatctccgac ttgacctccg
     4261 gattcagcat agaaaggagt tttgtcgaat acgaggtgaa acaacgtttt tttagcggtt
     4321 tttacttgcc ggtaacgggt tatgcgtact tcagtggttt caaaatcgta gccaaggaat
     4381 tcaacactgt cggaatttct gatttcaatc cagtcgccag cttcctgagc agctgcattc
     4441 cgtgaacggc ttttttgctg ttccatttcg gcattgaact gctcaatatt gaccgataaa
     4501 ttattttctt taaggatgag ttcggtgagg tcgagaggaa aaccgaacgt gtcgtaaagg
     4561 gtaaatgcat ctttgccatc aacttcgttt ttcccgtcgg cttttacttt ttggatcatg
     4621 ccgtcgagaa gcctgagccc agtttccaat gttttgagga acgattcttc ctcttcttta
     4681 atcactttcg cgatgaggtt ttcctgtgca accaattcgg ggaatgcatt gcccatttgg
     4741 tctttaagaa cagacaccag tctgtaaatg aatgcttccc tgaagttgag gaaagtgtag
     4801 ccatagcgca ctgcacggcg taaaatgcgg cggatcacat agcctgcctt attattcgaa
     4861 ggaagttgtc cgtcggcaat ggcaaacgat atagcccgga ggtggtcggc aataacgcgc
     4921 atggcaatat cttgttttgc gtcgatgcca tattttttcc cggcaagctt gccaatttca
     4981 gtgataatag tttgaaaaac atcggtatcg taatttgatt gtttaccctg aatcaccata
     5041 caaagtcttt caaaacccat gccggtgtcg acatgttttg ccggcaattc ttctagtttg
     5101 ccatttgctt tgcggttata ttgaatgaaa acattgttcc atatttcgat aacctgaggg
     5161 tggtcattat ttactaaatc tttacctgca atcaatgctc tctcactgtc ttgtctgatg
     5221 tcgacgtgta tttcagagca aggaccacag ggccctgtgt cgcccatttc ccagaaatta
     5281 tcctttttgt tgcccaaaag gatccggtct tctggaagga attgcttcca atattcaaaa
     5341 gcttcgttat ctcttgcaac gccgtcttct tcattgcctt caaatacagt ggcataaagt
     5401 ctgtcgggtg aaatgcccat tttatctact aaaaattccc aggcccaacc gatggcttct
     5461 tttttgaaat aatcgccaaa tgaccagtta ccgagcattt cgaacatggt gtggtggtat
     5521 gtgtcatggc caacttcttc aaggtcgttg tgttttcccg atacgcgcaa gcacttttgg
     5581 gtatcggcaa cacggcggta ttttgctggt tcgttgccca agaaaatgtc tttaaactga
     5641 ttcatccccg cgttggtaaa catgagggtt ggatcgtttt taacaaccat tggagccgag
     5701 ctaacaatct gatgttcctt gcttttgaaa aaatcgagga atgcttttct tatttcttga
     5761 gatgtcatat aacacaaaaa attattgaat tagaaaaaat agtccttagg aatttttaaa
     5821 aatattatta gttttgctac agatagggaa attttctatt tgagcgatgc aaaaatagga
     5881 attgtacatt tagaacgact attatttcca gtaatttttt gaatgatggg gggacataaa
     5941 taccatttta atccacagac actatgcttc gaaccaaaaa cgaagaagcg gagcaaaagt
     6001 gtaatgttaa ttttaggctt tttagttttt tttgttattg tatctattgc attctcaaca
     6061 ctttttgtat ctcacttcga aactcccgaa gtaaaatcat tgcgcaaaga aaatcaacag
     6121 cttttatctc aatataattt tctgaattct aaattgacac aacttgaaac agtacttgaa
     6181 gaacttcagg taagagacga caacatttat cgtgttgttt tagggaaaaa ccctatcccc
     6241 gaggatgtcc gcaaagcagg ttttggtggt gataaccggt acgcttcgct cgaacaactt
     6301 agcgatgcag aaattgtatt gaatacgtcg aagaaaatcg atgaaatatc gaaacaggct
     6361 tacattcagg caaaatcgta tgaggaagtt ttgaatattg ccttaaaaaa agatcgtgaa
     6421 atggcttgca ccccagccat tatgcctatc gcaaataaag atcttgacca tacttcgtca
     6481 gggtggggaa tgaggattca tccaatttat aaagttaaga tgtttcattg ggggatcgat
     6541 ttcgttgctc ccaaaggaac aaaagtatac tcaaccggtg acgggattgt aaaggaaact
     6601 ccttcattgc gagatggtca tggaaaacat gttgttattg accatggctt cggatatcaa
     6661 actctttatg ctcatctttc tgccttcaat gttaagcccg gtgataaagt gaagcgcggg
     6721 caggttattg ggtttattgg gagcacaggt acttcaactg ctcctcattt gcactatgaa
     6781 gtaaataaag acggaagaaa tgtagaccct aaatattatt tctttaaaga ccttacacca
     6841 gagcaatttg ctcagttggt tgcccgctcg tcgaataatg ggcaaagttt cgactaatgt
     6901 aaatcatcgt gccatacaaa caacctat
//
LOCUS       DAOPUW010000014         6907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_10941_length_6907_cov_3.070928, whole genome shotgun sequence.
ACCESSION   DAOPUW010000014 DAOPUW010000000
VERSION     DAOPUW010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10941_length_6907_cov_3.070928"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /gene="pyrB"
                     /locus_tag="PKJ43_00515"
     CDS             complement(<1..756)
                     /gene="pyrB"
                     /locus_tag="PKJ43_00515"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNZ71067.1"
                     /translation="MEKRDLISITDFSKADYLRIMELAAEFEANPNQELLKNKVVATL
                     FFEPSTRTRLSFETAINRLSGRIIGFSDSSSSSVVKGETLHDTIRMVSNYADMIVMRH
                     PLEGSARYASEVASIPVINAGDGANQHPSQTLLDMYSIKKTQGSLDNINIFMVGDLKY
                     GRTVHSLLMAMSEFENPIFNFIAPPELAMPDEYKRFLKSKNIRYFEHTQFTDIISEAD
                     IIYMTRVQKERFTDPIEYEKVKNVYILKKKMLKN"
     gene            complement(951..1565)
                     /locus_tag="PKJ43_00520"
     CDS             complement(951..1565)
                     /locus_tag="PKJ43_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNZ71068.1"
                     /translation="MSVQDTYQTVEGSAEGLFKDRGSKFIAFIFHVEKEEEIKETIQH
                     IKKEHFSARHHCYAYRLGYYGEKYRANDDGEPSGTAGKPIHGQLLSHNLTNTLIVVVR
                     YFGGTLLGVSGLINAYKNAAADAILNASIVTRIIENNYRLKFGYPLQNAVMKIIKDEQ
                     LEVVSASYELDCTLEIKVRINKTDTVTEKLATIIGLTVEPLLCE"
     gene            complement(1605..3032)
                     /gene="dnaA"
                     /locus_tag="PKJ43_00525"
     CDS             complement(1605..3032)
                     /gene="dnaA"
                     /locus_tag="PKJ43_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNZ71069.1"
                     /translation="MLKSHHQIWNDCLSIIKDNVQPVSFRTWFEPIKPLKIDNEILTI
                     QVPSPFFYEYLEEQYIDILRKTLRKVIGANAKLEYNVVMSNVPQKSGSLTVNYPTNNN
                     LKLNNRPVSVPVTADTASIKNPFVIPGIQKLHIDPQLKKDNTFENFIEGECNRLARSA
                     GFAVAQNPGGTAFNPLMIYGDSGLGKTHLAQAIGIEVKERFPEKVVLYVNANKFSTQY
                     QEATRNNTRNDFMHFYQMIDVLILDDVQEFSGKEKTQETFFHIFNHLHQLGKQLILTS
                     DKSPIEIKGIEQRLLSRFKWGLTTDLQSPDYETRLAILKKKTYKDGLEMPEKVLEHIA
                     QHISNNVRELEGALISLLAQSTLNRKAITIDLAIDMINKIVKQNKHEITIDYIQKIVC
                     DYFNMPIDSLCSKTRKREVVQARQIAMFFSKNLTKSSLASIGSQIGRKDHATVLHACK
                     TVTNLMDTDKQFRLDVEEIEKRLKV"
     gene            complement(3644..4849)
                     /locus_tag="PKJ43_00530"
     CDS             complement(3644..4849)
                     /locus_tag="PKJ43_00530"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNZ71070.1"
                     /translation="MLRITINTIVIALLLSLSANMYGQKNDYSIKKIVIDPGHGGKDP
                     GATVGKTYEKDIALDVSLRAGKMIKKMYPEVEIIYTRDKDIFIPLGDRPEIANKNKAD
                     LFISVHVNICGTPGTTGAETYILGQHRSQENLEVAKLENAVILREDDYNTRYEGFDPN
                     SSESYIMFELLQNEHLEHSRLFADRVQNHFVSHAKRKDRGVRQAGFLVLRKIAMPSVL
                     IELGFMSNKDEKAFLEKDQGKQLLAEAIVKSFSEYKSRFDEQKEPPKPPVVQAIEKEK
                     KEITEEPKVQPVETKDTKEVKETQQTTENKEQKGDSTQLSDDLSKIQGKWYGTQIMAL
                     KKKYNPKDAMFKKFEPVYYFNENGYYKYVLGLANNIDEATKQHQEIKKSFNGAFMVSF
                     ENGVRSVID"
     gene            5021..>6907
                     /locus_tag="PKJ43_00535"
     CDS             5021..>6907
                     /locus_tag="PKJ43_00535"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HNZ71071.1"
                     /translation="MNRIQHIFLVFVFMLLSVRVLAFSWADSVGNEPFVPQNAEKLLV
                     NDSLSFAADSSSQLAKTDTTETVAPIFESPISYNAVDSMVVSLEDSNQVVYLYGKANL
                     KYQEIDLVADIVEVNLGTKEIFAKGLVDSLGNQLSKPHFKQGTDEFDCTSLRYNFETQ
                     KGFVENVITNREDGKVHGAKAKMVSKDIFCMVDGKYSTCDADHPHFYLHISKGKLIGD
                     KAIIAGRSYIVIEDFPLYFPFLPYGYIPTNKKTYSSGIILPSYGYLQDSRGSVLRDGG
                     FYWAASDYFDVKLTGEIYSKGSWGVGFESRYKLRYKFSGSFSFNYKMTIMGEKGIDQQ
                     KKPNLSLKWSHSQDSKSNPTLSFSGDVSFSTSGYNKMHEYNNTEQYLQNQTNSRVSLT
                     KKFPNSPFTLSLSMSASQSSSNKTLDLSLPNISFDMRTIQPFKRKKPVGSKRFYEDFK
                     LSYSTKIQNSVKVKEGELFSTPLSKWKKSVTHSIPITLPSFRVLNYINVTPSVNYSEQ
                     WYFDYVEKYWVDGYMDRDPETGIERWQKGYVAQETKYGFKRNYNYNFGISGTTTLYGM
                     YQMRNPSSKLTAVRHKLDLTLGLNYQPDLTDPRHGFNKMVQVDSLGRMQLYNIFSSSS
                     PTMNG"
BASE COUNT         1865 a         1279 c         1380 g         2383 t
ORIGIN      
        1 gtttttcaac atctttttct tgagaatata aacattttta actttctcgt attcaatagg
       61 atctgtgaat ctttcttttt gaacacgggt catgtaaatg atatcggctt ccgaaatgat
      121 gtcagtaaac tgggtgtgtt caaagtaccg tatgtttttg cttttcagga atcgtttgta
      181 ttcatcgggc atagccagtt cgggtggggc aatgaaattg aatatcgggt tttcgaattc
      241 agacatggcc atgagcagcg agtgtaccgt tcgtccatat tttaaatcgc ctaccatgaa
      301 gatgttgata ttgtcgaggc ttccctgtgt tttttttatc gaatacatat cgagcagagt
      361 ttgcgaagga tgctggttgg caccgtcgcc agcattgatt accggtattg atgctacttc
      421 gctggcgtaa cgtgcgctac cttccagcgg gtgccgcatc acaatcatat cggcataatt
      481 gcttaccatg cggatggtgt cgtgaagggt ttcgccctta actacgctcg acgatgatga
      541 atcagagaag cctataatcc tgccacttag tcggtttatg gctgtttcga agctgagccg
      601 ggtccttgtc gatggctcga aaaacagggt ggcaactact ttgtttttta gcagctcctg
      661 gtttggattg gcttcaaatt cggcggcaag ttccataatc ctcagatagt ctgctttgct
      721 gaagtcggta atcgatatca aatctctttt ttccatggtc agaaaatttc atgatttttt
      781 ggataaaaaa aatgccaacg tgtaatttgt tggcagataa tattgttgat agcttttgct
      841 gaacgcgata gttggttggc gtccgaactc aatgctatgg tatttttcat attcatcgct
      901 ggctaattta ctattattcg aaaaagaaaa ccatgattcg tccttgtttt ttattcacaa
      961 agtaaaggtt caactgttaa tccgattatc gttgccaatt tttcggtgac ggtatcggtt
     1021 ttgttaatcc gtacttttat ttcaagcgtg caatcaagct cataactagc cgaaactact
     1081 tcaagttgct cgtcttttat tattttcatt acagcatttt gcagtggata gccaaatttc
     1141 agccgatagt tgttttctat tatgcgggtc acaatagaag cattcagtat tgcatcggcc
     1201 gctgcatttt tgtaggcatt aataagcccg ctgacaccca gcaaggttcc tccaaaataa
     1261 cggactacca caataagtgt gtttgtaagg ttgtggctaa gtagttgtcc gtgaattggt
     1321 tttccggcag tgccagaagg ttctccatcg tcgttggcgc ggtatttttc cccatagtat
     1381 cccagccggt aggcataaca atggtggcgg gctgaaaagt gttctttttt aatgtgttga
     1441 atggtttctt ttatttcttc ttctttttca acgtgaaaaa taaaggcaat aaacttactc
     1501 cctctgtctt tgaagagccc ttcggcactg ccttcaacag tctggtatgt gtcttgcacg
     1561 ctcacgataa aaaaaaagca gatcgtttga cctgcctgag tgttttatac ttttaatcgt
     1621 ttttctattt cttcaacatc aagcctgaat tgtttgtcgg tgtccatcag gttagtaaca
     1681 gttttgcatg catggagcac cgtggcatgg tcttttctcc caatttgtga tccaatgctg
     1741 gctaacgacg attttgtgag gttcttcgaa aagaacatag caatttgccg ggcttgtacc
     1801 acttctcttt ttcttgtttt cgaacaaagc gaatcgatag gcatgttgaa ataatcacat
     1861 acaatttttt gaatgtaatc gatggttatt tcgtgcttgt tttgctttac aatcttgtta
     1921 atcatgtcga tggcaagatc gatagtaatg gcttttcggt taagtgtcga ttgagccaac
     1981 aacgaaataa gtgcaccttc gagttcgcga acgttattgc tgatgtgttg ggcaatgtgc
     2041 tcgagaacct tttcgggcat ttcaagcccg tctttgtatg tttttttctt caggatagct
     2101 agccgtgttt catagtcagg cgactgcaaa tcggtagtta gcccccattt gaaacgcgaa
     2161 agcaagcgtt gctcaatccc ttttatttca attggcgact tgtctgacgt aaggataagt
     2221 tgtttgccta attgatgtaa gtgattgaat atgtgaaaaa aggtttcttg tgtcttttct
     2281 ttgcccgaaa actcctgcac atcgtccaga atgagaacat caatcatttg gtaaaaatgc
     2341 ataaagtcgt ttcgggtatt gttccgggtt gcttcctgat attgtgtgct gaatttgttc
     2401 gcatttacat agagtacaac cttctcgggg aaacgttctt taacttcaat accaatcgct
     2461 tgggccaggt gtgtttttcc aaggccacta tcgccatata tcataagtgg gttaaaagct
     2521 gtgcctccgg ggttttgagc aacagcaaag cctgctgaac gagccaaacg gttgcattcg
     2581 ccttcgataa agttttcgaa tgtgttatct ttcttaagct gcgggtcgat gtgtaatttt
     2641 tgaattccag gaattacaaa aggatttttt attgatgcag tatcggcagt tactggaaca
     2701 gaaactggtc gattgtttaa tttaaggttg ttgttggttg ggtagttaac agtgagtgag
     2761 ccgctctttt gaggaacatt gctcatcacc acattatact ccaatttagc atttgcccca
     2821 ataactttcc gcaacgtttt ccgaagaata tctatatact gctcttctaa atattcatag
     2881 aaaaatggac taggaacttg tatggtaaga atttcattgt cgatcttaag tggtttaata
     2941 ggctcaaacc atgttctgaa gctaacgggc tgaacattgt ctttgatgat cgataaacaa
     3001 tcattccata tctggtgatg actctttaac attactggca atttggtttc aaaaatttaa
     3061 gtttagtttg cgactactgt ttaacgaatg acaaaaatgt gaaaaaataa tctgaaaaaa
     3121 aatagtttga agattagttt ttttaaattt tttttaaatg cttatgattc aaatttatag
     3181 aaataaacat tttttaaata attgaaaatg agaattttaa atttgcataa aaatgataaa
     3241 aggttgaata aatggtaaat aataacccag ttaatataat tttgaatatg acttttaata
     3301 ttatgataaa aaagaattag catatactat taagatctac tttatttgaa agttatttat
     3361 tcttgttgta ctcggttttc ttatcgaatg ggatttcttt ttggtggttt tgtcgtgtcg
     3421 ttttatccat atctttgtaa taaaccacta acaatattgt attaatagta cttttacttt
     3481 ttaagagggt taaatatttt tttaagttta ttttttgata aaaagaaaat gtctaatctt
     3541 ttctaaatgt atggatgttc tatgttgtgg aatttgttgt tttatatttg cttgttacaa
     3601 tgtttgttat cttttttgtt tactgagtga ttcttttatg ttgttaatct attacactgc
     3661 gcactccatt ttcgaaggat accataaatg ccccgttgaa cgatttcttt atttcttgat
     3721 gctgttttgt agcttcatcg atattattgg ccaaccccaa cacatatttg tagtacccgt
     3781 tttcattgaa ataatagacg ggttcaaact ttttaaacat agcatccttg gggttatatt
     3841 ttttctttag tgccataatt tgggttccgt accatttccc ctgaattttg gataagtcat
     3901 cacttagttg agtactgtct cctttttgtt ctttattttc agtggtttgt tgtgtctctt
     3961 ttacctcttt tgtgtctttt gtttcaactg gctgaacttt tggctcttct gttatctctt
     4021 tcttttcttt ttcaatagct tgaacaacag gaggttttgg tggttctttc tgttcatcga
     4081 acctgctttt gtattcagag aaagacttaa caatggcctc ggccagtagc tgctttccct
     4141 gatctttttc caaaaaggct ttttcatctt tgttgctcat gaagcctagt tcgattaaaa
     4201 cactgggcat tgcgattttt cgtagaacca agaagccagc ttgcctaacg cccctgtctt
     4261 ttctctttgc atggctcacg aaatggtttt gaaccctgtc ggcaaaaagg cgactatgtt
     4321 cgagatgttc attctgaaga agctcaaaca tgatgtatga ttccgatgag ttagggtcaa
     4381 atccttcata acgtgtattg tagtcatctt cgcgcaagat taccgcattt tcaagttttg
     4441 caacttcaag gttttcctgg cttcggtgtt gtcccaaaat gtaagtctcg gctccggtag
     4501 tgcccggggt accacatata ttcacgtgta ccgaaataaa taaatcggct ttgtttttat
     4561 tggcaatttc tggacggtcg cccagcggga tgaaaatgtc tttatcgcgg gtgtaaatta
     4621 tttctacctc gggatacatt tttttaatca tttttccggc acgcagcgag acatcgagtg
     4681 caatgtcttt ttcgtatgtt ttacccactg ttgccccggg gtctttaccc ccgtgccccg
     4741 gatctattac tatttttttt attgagtaat cgtttttctg accatacatg ttggccgata
     4801 acgaaagtag caaggcgatt actattgtat ttattgtaat tcttaacatt ttttggctct
     4861 cttttaataa taatctatat caggaaaagt ttaccttagg ccactgtaaa gtaaattgta
     4921 aattctcaat gatgcaacgg ctgaccacga taaaaccttc tatttggtgt aaacggtttc
     4981 taattttaat atatttttgg aacttttaaa aatagttgta ttgaacagaa ttcaacacat
     5041 attccttgtt tttgttttta tgttattatc tgtcagggtt ttagccttta gttgggccga
     5101 ttctgtgggt aatgaacctt ttgtacctca aaatgcagaa aaacttctgg taaatgattc
     5161 actctctttt gctgccgatt ccagttcaca acttgctaaa accgacacta ctgaaacagt
     5221 tgcccctatt tttgaatcgc caataagtta taatgctgtt gattccatgg tggtttctct
     5281 cgaagatagc aatcaagttg tttatctgta tggtaaggcc aatcttaaat atcaagagat
     5341 tgatttagtg gcagatattg ttgaagtaaa tcttggaacg aaagaaattt tcgccaaagg
     5401 cctggtcgat tctctgggga atcaactctc gaaaccccat tttaagcaag gaactgatga
     5461 gttcgattgc acttcgttgc ggtataattt cgagactcaa aaaggttttg ttgaaaatgt
     5521 tattaccaat agggaagatg gaaaagttca tggagctaaa gcaaaaatgg ttagcaaaga
     5581 cattttttgt atggtcgacg gtaaatattc aacatgcgat gccgatcatc cgcatttcta
     5641 tctgcatatt tcgaagggta aactaattgg tgataaggca attatagctg gtcgatcgta
     5701 catagtcata gaagatttcc ccctttactt cccgtttttg ccatacgggt atattccgac
     5761 caacaaaaaa acatatagtt cgggaattat actcccttca tacggatatc ttcaggattc
     5821 gcggggctcg gtgcttcgcg atggcggatt ttattgggct gcctccgatt atttcgatgt
     5881 aaagcttacg ggtgaaattt attccaaagg ttcatggggt gttgggttcg agagccgcta
     5941 caagcttcgc tacaagttct cgggcagttt ttcgtttaat tacaaaatga ccatcatggg
     6001 tgaaaagggg atagaccagc aaaaaaaacc gaacctctca ctaaagtgga gccattcaca
     6061 agactcaaaa tcgaacccaa ctctgagctt ctcaggggat gttagctttt caacatcggg
     6121 atacaacaaa atgcatgaat ataacaacac cgagcaatat cttcaaaacc aaacaaactc
     6181 aagggttagc ttaacgaaga aatttccaaa ttccccgttt accttatcgt taagtatgag
     6241 tgcctctcag agttcttcca acaaaacgct cgatttgtcg ttgcccaata ttagctttga
     6301 catgcgtacc attcagccat ttaaacggaa aaagccagtt gggtccaaac gtttctacga
     6361 agattttaag ttatcctatt ctaccaagat ccaaaattcg gtaaaagtca aagaaggtga
     6421 attattctca actccgcttt ctaaatggaa aaaaagtgta acgcattcta ttccaattac
     6481 attgccttcg tttagggtgt tgaattatat caacgttacc cccagtgtga attattctga
     6541 acaatggtat ttcgactatg tagagaaata ttgggtcgat ggatatatgg atcgtgaccc
     6601 tgaaacaggt atcgaacgct ggcaaaaagg ctatgttgcc caggaaacaa agtatggctt
     6661 taaacggaat tataattata actttggaat tagcggaacg acaacgcttt acgggatgta
     6721 ccaaatgcga aacccaagtt caaaacttac tgcggtgagg cataaactcg acctaacgtt
     6781 gggtctgaac tatcagcccg atttaactga tccgcgtcat ggcttcaata agatggtaca
     6841 agtagattcg ctggggcgta tgcaattata taacatcttt tcttcttcga gtccaacaat
     6901 gaatgga
//
LOCUS       DAOPUW010000015         6538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_11720_length_6538_cov_3.288601, whole genome shotgun sequence.
ACCESSION   DAOPUW010000015 DAOPUW010000000
VERSION     DAOPUW010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11720_length_6538_cov_3.288601"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="PKJ43_00540"
     CDS             complement(<1..146)
                     /locus_tag="PKJ43_00540"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNZ71072.1"
                     /translation="MSAAIDDDKRKVTTHRLKEMKNRGEKISMLTAYDFSMASIIDQA
                     GMDV"
     gene            659..2668
                     /locus_tag="PKJ43_00545"
     CDS             659..2668
                     /locus_tag="PKJ43_00545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ71073.1"
                     /translation="MKTFLKITLLALSISICAITNSYAQEIPYVYDVENTGADFPVPP
                     LPSFDQLPIVKPLTDPFEWSDGSGRDTTFESWSRRRSEIKAEFEHYEIGVKPVRPDTI
                     TATYSAGLLTVNVTENGQTITLTSNITLPEGQGPFPAIIGMNSGSGSLPADIFTSRNV
                     ATVPFFHNQVVTYGGKSKSDPFYKLYPDLYYVGQYASWAWGISRLVDGLELVQDSLPI
                     DLSHLAISGCSYAGKMALIGGALDERFALVIAQESGGGGAAAWRVSETLTGVESLGNT
                     NFTWFMQSLSQFKGSAIERLPHDHHELMAMVAPRALLVLANPDMVWLADESGYVSTKA
                     AQRVWKTFGVENRFGYVIEGGHSHCVVSNNQRQSVETFVERFLLGDTAVNTNIEIHTY
                     PDVDYQRWTNWWGNGEPEFLSRDWLGTESIWLEAECGTVGSKWTPVKYPIGSNASVVE
                     VVKDAEPVSSVPVNTESVLEFPFTTTRDTTYHFFMRLICAKAEAGEYWIKVDDGDYQK
                     VTGLTTSGLQWKKLLQKELAAGDHLLKVAYSQRGAKIDKFCVSSLKSTPAADAGETAV
                     NICEPQYVSSEIIRRNGFVLDQNYPNPYNGKTLISFEVPTESFVSLKVYDMQGAEITE
                     LAGKQYATGKHVVEFNSGNLPKGMYFFTMNAGGISLSRKMIISGE"
     gene            2940..3251
                     /locus_tag="PKJ43_00550"
     CDS             2940..3251
                     /locus_tag="PKJ43_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNZ71074.1"
                     /translation="MLRVKSICRQQGITLKELAERMAVSPEVVTRVLSDSGNPTLSTL
                     INMAKALNVNVSELFEDFNADMLVRGYLEIGDQTHRINNFSDLQTIYNKLSARDGSGN
                     L"
     gene            3232..5061
                     /locus_tag="PKJ43_00555"
     CDS             3232..5061
                     /locus_tag="PKJ43_00555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNZ71075.1"
                     /translation="MDQEIYNQLVSFIWNIANDVLVNTYNKGDYRKVILPMMVIRRLD
                     AVLEPTKDAVLAMKKSLDEAGVLNQEEALCSVASQAFCNFSPFMMKQLKARTNRQQLK
                     ADFIAYLDGFSENVQDIITKFELRNQIDKLSEGGILGMLIEKFVDPKINLSTKPVLDD
                     EGNEKLPGLDNHSMGTIFEELLRRFNEENNVTEAGEHFTPRDIVELMADLAFAPIIDQ
                     ILDSTYLIYDGACGTGGILSESDARINYLANKNGKKINTHIYGQELQPETYATCIADL
                     LIKGEGEEAKNIAFGSTISEDGHSGKTYDFLISNPPFGTPWKKDLEKWGYKDKKEITD
                     RRFVRQYGSHVQYSFVPNIGDPQMLFLANNVSKMKSNTELGSRIVEVHNGSSLFTGNA
                     GGGESNLRRYIIENDLLEAIIAMPEKMFYNTGIATFLWIVTNRKSEKRKGKVQLIDAT
                     SIKTALRKNLGEKNCKVAKADRKKIMQTYLEFEENEYCKIFPNEEFGFWEVTVEQPLR
                     DENGAVVTDKKGKPKMDNALKDTEQIPLLYEGGIDAFLEKEVYPYTPDAFVDKEKATI
                     GYELSFTKYFYKPITLRSLDAIKADLQLIESETDGLLNEILGV"
     gene            5064..6431
                     /locus_tag="PKJ43_00560"
     CDS             5064..6431
                     /locus_tag="PKJ43_00560"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNZ71076.1"
                     /translation="MEQELNPYPEYKSVNLNWILQIPEHWNCERAKNMFDKLNRAVED
                     TDETITCFRDGVVTLRKNRRITGFTESLKEIGYQGIRKGDLVIHQMDAFAGSVGVSDS
                     NGKSTPVYSVCVPKNDKYNNYYYAHLIREMGKNGFIQSLYRGIRERSSDFRFDTFAKL
                     FLPIPPRPEQDQIVKFLDFKISKINKFIKDKKREIELLKELKQAEINRAVTKGLNPNV
                     PMKDSGIDWLGEIPEHWEVRRLKSFSDFVNRGSTPLYTEDILYKVVNQATFSKGFWDL
                     KNIRYTSASIENNRGLLLKEDILLASTGGGVLGKCYLFKEEGIYIADSHVSIIRDSKS
                     RFHPEYIYYFLSINYHLINGILAQGSTNQIELQREWLRSMFFPYPDIKEQLEIATFIV
                     GLEDKINNLIESIESEIIKIQEYKISLISDVVTGKVDVRDVSIEDAFEMETLDEVETE
                     EIVEE"
BASE COUNT         2078 a         1107 c         1389 g         1964 t
ORIGIN      
        1 attacatcca tgcctgcctg gtcgattatc gacgccatgg aaaaatcgta agcggtcagc
       61 atcgaaattt tttctccacg attcttcatt tctttaaggc gatgcgtggt aacctttctt
      121 ttatcatcat ctatagctgc tgacatatta ttcttattaa aatgaaaaac aaaattacac
      181 tagattacta aaccaagtaa cttttttgtg aatttgttgt gctggtaagt tgccgttttt
      241 ttgtactaaa agccagattg ctttttttcc ttttgtgttg aatgtaaatg aatgatattt
      301 agatgcatgg tgtttagtgg attgttttct ctccagggtc ttaattttag tattcaagaa
      361 tagtaaacca attttgcttc ataaattcat gttaatgttg aataattaat atgtgaggct
      421 gtaatatttt tctatctttg gtatctatgc aaccgattgc ataaaattga atatttatta
      481 aaagtaattt actaactaat ctttatgaaa aaattgtact tgtacaaaaa gaaacaacct
      541 cccattctgc caaggaataa tgaatttgtc gatgtaaatg atccgcctga tagttagtat
      601 tttaattcag ggctgagttt atttattcaa atttatttac atgataaaac attatttaat
      661 gaagacattt ttaaaaatta cgttgttggc gctcagtatc agtatttgtg ccattacaaa
      721 ttcgtatgcg caagaaatac cctacgtata tgatgttgaa aataccggag ctgattttcc
      781 tgtgccacca cttccatctt tcgatcaatt gccaattgtt aagccactta ccgatccttt
      841 tgaatggtct gatgggagtg gccgcgatac aacttttgaa agctggagcc gtcgccgttc
      901 tgaaattaaa gctgaattcg agcactacga aattggggta aaaccagttc gccccgacac
      961 cattactgca acatattcag cgggcttgtt gactgtaaat gttaccgaaa acgggcaaac
     1021 tattacctta acttcgaata ttaccttacc cgaaggtcaa ggccctttcc ctgcaattat
     1081 tggaatgaac agtggttcgg gcagtttgcc tgccgatatt tttacatcac gtaatgtggc
     1141 tactgtccca ttttttcata atcaggtggt tacttatggg gggaaatcga aaagtgatcc
     1201 attttacaag ctgtaccccg atttgtatta cgtgggacaa tatgcttcgt gggcatgggg
     1261 aatcagccgc cttgttgatg gactggagct ggtccaggat tcgttaccta tcgatttatc
     1321 ccatctggca atttctggtt gttcgtatgc cggaaaaatg gcattgatag gaggtgcact
     1381 tgatgaacgt tttgctcttg taattgctca ggaatcgggt ggcggcggtg cagctgcatg
     1441 gcgtgtttcc gaaacgctta ccggtgtcga aagtcttgga aacactaact ttacatggtt
     1501 tatgcagagt ttgagccaat ttaaaggttc ggctatagaa agacttccgc acgatcacca
     1561 tgagcttatg gctatggtag caccacgtgc gttgctggtc cttgcaaatc ccgatatggt
     1621 ttggttggcc gatgaatcgg gatatgtttc gacaaaagct gcacaacgtg tctggaaaac
     1681 ctttggggtt gaaaatcgtt tcggatatgt aattgaagga gggcatagtc attgcgtggt
     1741 atcaaacaat caacgacaaa gtgtggaaac gtttgttgaa cgattcttgt taggtgatac
     1801 agcagtaaat acgaacattg aaattcatac ttatcctgac gtagattatc aacgctggac
     1861 taactggtgg ggtaacggtg agccggaatt tctgtcacgc gattggttgg gaaccgaatc
     1921 tatctggctc gaagctgaat gtggaacggt tggcagcaag tggactcctg taaagtatcc
     1981 cattggctct aatgccagtg ttgttgaggt tgttaaagat gctgagcctg tttcctcggt
     2041 tccagtaaat accgagagtg tgttagaatt tccttttaca acgactcgtg acactacgta
     2101 tcactttttt atgcgcttga tttgtgctaa agccgaagcc ggtgagtact ggattaaagt
     2161 tgatgatggc gattatcaaa aagttactgg tcttacaaca tcgggattgc agtggaaaaa
     2221 gctgttgcag aaagaattag cggcaggtga tcatttgttg aaagtggcat atagccagcg
     2281 aggcgctaaa atcgataagt tttgtgtctc tagtttaaag agcacccctg ccgccgatgc
     2341 tggtgaaaca gcggttaata tttgcgaacc tcaatatgtt tcttctgaaa taatacgtag
     2401 aaatggtttt gttctcgatc agaattatcc taacccttat aatggcaaaa cgctcatttc
     2461 gtttgaagta ccaactgagt cgtttgtgtc gttaaaggta tatgatatgc agggtgccga
     2521 aattactgaa cttgcaggaa aacaatatgc tactggaaaa catgttgttg aatttaactc
     2581 agggaacctt cctaaaggaa tgtacttttt caccatgaat gctggcggta tttcattaag
     2641 ccgcaagatg atcatttctg gtgaatagta gaaatagatg acacagaagg agggcctaaa
     2701 tgactctcct tttgttttat tagagaaagg tctaaaacat gatcatcggc tttgaccgaa
     2761 ggatcgaata tatataattt accatttcca tactgttgat ttatgtgtta ttttatacgc
     2821 gcaaatatag cacataaatc aatgcgcacc aaataatatt gattcaaaaa acaattttat
     2881 gtgaaaatat tgacttatga attatttttt ttaagtttgt gattgagata aagtataaaa
     2941 tgttacgagt taaaagtatt tgccgacaac aagggatcac tttaaaagaa ttggcggaac
     3001 gaatggctgt ttcacccgaa gtggtaacac gtgttttatc cgatagtggt aatcctaccc
     3061 tttcaacttt aatcaatatg gctaaagcat taaatgtaaa tgtttctgaa cttttcgaag
     3121 attttaatgc cgacatgtta gtccgtggtt atcttgaaat tggagatcaa acccatcgga
     3181 taaataattt tagcgatttg caaaccattt ataataaact aagcgcacga gatggatcag
     3241 gaaatttata atcaattggt atcgtttatt tggaatattg ccaacgatgt tttagtaaat
     3301 acatataata aaggagatta caggaaagtt attttgccca tgatggttat ccgtcggttg
     3361 gatgcagtgt tagaacctac aaaagatgcc gtgctagcaa tgaaaaagag ccttgatgaa
     3421 gcaggcgtgt tgaaccagga agaagcgttg tgcagcgttg cttctcaggc attctgtaat
     3481 ttctccccgt ttatgatgaa acaattgaaa gcccgtacca acaggcaaca attgaaagcc
     3541 gatttcattg cttatctcga tggttttagt gagaatgttc aggatattat taccaaattc
     3601 gaacttcgta atcaaattga caaactgtcc gaaggtggaa ttcttggaat gctgatcgaa
     3661 aaatttgttg atccgaaaat taaccttagc accaaacccg ttcttgatga tgaaggtaat
     3721 gaaaaattac ctggacttga taatcattca atgggtacaa ttttcgaaga actgttgagg
     3781 cggttcaacg aagaaaacaa tgtaaccgag gcaggggaac atttcactcc acgggacatt
     3841 gttgaactga tggccgattt ggcatttgcc cccatcattg atcaaattct cgatagtacc
     3901 tatcttattt acgacggagc atgtggaacg ggtggtattt tatcggaatc ggatgcccga
     3961 attaactatt tggccaacaa aaacggaaaa aaaatcaata cccacattta cggacaggaa
     4021 ctccaacccg aaacttatgc aacatgtatt gccgacttgt tgattaaagg tgaaggcgaa
     4081 gaggctaaaa acattgcctt tggttcaacc atttcggaag atggtcattc gggtaaaacc
     4141 tatgattttc tgatatcgaa tccgccattt ggaacaccgt ggaaaaaaga tttggaaaaa
     4201 tgggggtata aagataaaaa agagattacc gaccgacgct ttgtaagaca atacggcagc
     4261 catgttcaat actcgtttgt gcccaacatt ggcgaccccc agatgctttt tttagccaac
     4321 aatgttagta aaatgaagag taataccgaa ctgggtagcc gcattgttga ggttcataac
     4381 ggatcatcac tttttacagg caatgctggt ggtggcgaaa gtaaccttcg gcgatacatt
     4441 attgaaaacg acttactgga agccataatt gccatgcccg aaaagatgtt ctataatact
     4501 ggtattgcta catttttatg gattgttacc aaccgtaaat cggaaaaacg taagggtaag
     4561 gtgcaactaa ttgatgccac atctattaaa acggcattac ggaaaaatct tggtgagaaa
     4621 aattgtaagg tagccaaagc cgaccgaaaa aaaataatgc agacctatct tgaattcgag
     4681 gaaaacgaat actgtaaaat attccctaac gaggaatttg gtttttggga agtaacggtt
     4741 gagcaaccct tgcgcgatga aaatggtgcg gttgttaccg acaaaaaggg taaaccgaaa
     4801 atggataatg ctttgaaaga tacagaacag atacctttgt tgtacgaagg gggcattgat
     4861 gcctttctgg aaaaagaagt atatccttac acccctgatg cttttgtaga taaagagaaa
     4921 gctaccatag gttatgagct gagttttacc aaatacttct ataaaccaat aaccttacgt
     4981 tcgctcgatg cgattaaagc cgatttgcag ttgatcgaaa gtgaaactga tggattgttg
     5041 aatgaaattc tgggggtgtg aatatggaac aagaattaaa tccatatcca gaatataagt
     5101 ctgttaatct aaactggata ttacaaatac ctgaacattg gaattgcgaa cgtgcaaaaa
     5161 acatgttcga caaactgaat cgcgcagttg aagatacaga tgaaacaata acatgcttta
     5221 gggatggagt tgttacattg agaaagaaca ggcgaataac tgggtttact gaatcactga
     5281 aagaaattgg ttaccaagga ataaggaaag gcgatttagt tattcatcaa atggatgcgt
     5341 ttgctggttc agttggggtt tctgattcaa atggaaaaag cacaccagtt tatagtgttt
     5401 gtgttcctaa aaacgataaa tacaacaact actattatgc ccatttaatc agagaaatgg
     5461 gcaagaatgg atttattcaa tcattgtata gggggattcg tgaaagatct tcagatttta
     5521 gatttgatac gtttgcaaaa ctatttcttc ctatcccgcc ccgccccgaa caagaccaaa
     5581 tcgttaaatt ccttgatttt aaaatctcaa aaatcaacaa gtttatcaaa gacaagaaac
     5641 gtgaaattga gttgttgaaa gagcttaaac aagccgaaat taaccgtgcc gtcaccaaag
     5701 gcttaaaccc caatgtgccc atgaaagata gtggcattga ttggttaggg gaaattccag
     5761 aacattggga agtaaggaga ttaaaatcat tttctgattt cgtaaatcgt ggttctactc
     5821 cattatatac tgaagatata ttgtataaag ttgttaatca agcaactttt agtaagggat
     5881 tctgggattt aaaaaacatt cgatatactt ctgcaagcat agaaaataat agagggcttc
     5941 tattaaaaga agatatttta ttagcatcaa ctgggggtgg agtactaggc aagtgttatt
     6001 tattcaaaga agaaggaata tacattgcgg atagtcatgt gtcaattatt cgggactcta
     6061 agagcaggtt tcatccagaa tatatctatt attttctttc gattaattac catttaataa
     6121 acggaatttt agcacaagga tcaacgaatc aaatagaatt acaaagggaa tggttgcgaa
     6181 gtatgttttt cccttatcca gatattaaag aacaattgga aatagcaaca tttattgtcg
     6241 gtcttgaaga taaaattaat aaccttatag aatccattga gagtgaaata ataaagattc
     6301 aggaatacaa aatcagcctt atcagcgatg tggtaacagg aaaagtggat gttcgggatg
     6361 taagcattga agatgctttt gaaatggaaa cattggatga ggttgaaaca gaagagatag
     6421 ttgaagaata atgcactacc gatatgattt cgaaagatga acttgaaaaa ctgttgctcg
     6481 acctagagaa ttcacggatt gaacgtacca tttcgcttac caataccgat aaatttgg
//
LOCUS       DAOPUW010000016         6486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_11841_length_6486_cov_3.308506, whole genome shotgun sequence.
ACCESSION   DAOPUW010000016 DAOPUW010000000
VERSION     DAOPUW010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11841_length_6486_cov_3.308506"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /gene="rplF"
                     /locus_tag="PKJ43_00565"
     CDS             complement(<1..511)
                     /gene="rplF"
                     /locus_tag="PKJ43_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNZ71077.1"
                     /translation="MSRIGKLPIEIPGGVTVKVDDNNVVTVKGPLGELSQKVNPEIDV
                     KIEGNQLTVNRPSDEKNHRAMHGLYRSLLNNMVTGVSKGFEVKLELVGVGYRAENQGQ
                     VLQLVLGYSHHIFLQLPSEVKVEAVTDKRSNPTITLKSYDKQLIGQVAAKIRSFRMPE
                     PYKGKGIKFV"
     gene            complement(530..928)
                     /gene="rpsH"
                     /locus_tag="PKJ43_00570"
     CDS             complement(530..928)
                     /gene="rpsH"
                     /locus_tag="PKJ43_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNZ71078.1"
                     /translation="MTDPISDYLTRLRNAIKAGHKVVDVPASNLKKEITKILKEKGYI
                     LNYKFEDGVNQQGNIKIALKFHPETGDSAIKKLERISKPGLRKYCNSEDVPRVLNGLG
                     IAIISTSKGVITDKEARDLNVGGEVLCYVY"
     gene            complement(948..1217)
                     /gene="rpsN"
                     /locus_tag="PKJ43_00575"
     CDS             complement(948..1217)
                     /gene="rpsN"
                     /locus_tag="PKJ43_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNZ71079.1"
                     /translation="MAKESMKAREVKRAKLIQKYAAKREELKANGDYIGLQKLPKNSM
                     PIRLHNRCKLTGRSKGYMRQFGISRINFREMASQGLIPGVKKASW"
     gene            complement(1221..1778)
                     /gene="rplE"
                     /locus_tag="PKJ43_00580"
     CDS             complement(1221..1778)
                     /gene="rplE"
                     /locus_tag="PKJ43_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016779051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNZ71080.1"
                     /translation="MEFVPSLQKKYTEKVVPALMKEFGYKSIMQVPRIEKIVLNQGLG
                     AATADKKIIEAATEEMTNIAGQKCVQTKSRKDISNFKLRKKMPIGVRVTLRRQRMYEF
                     LERLIVVALPRIRDFKGIEGKLDGRGNYTLGITEQIIFPEIVLDKVNRLTGMNITFVT
                     TAKTDEEGYALLREFGLPFKNEKKD"
     gene            complement(1778..2101)
                     /gene="rplX"
                     /locus_tag="PKJ43_00585"
     CDS             complement(1778..2101)
                     /gene="rplX"
                     /locus_tag="PKJ43_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016779052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNZ71081.1"
                     /translation="MQKKLKIKKGDTVVVVTGNSKGQKGKVLEVNRDNDRAIVEGVNM
                     VKKHTKPNSKAPQGGILEKEASIHISNLMVADPKTGKPTRVGRRMDADGNLVRYSKKS
                     GEEIK"
     gene            complement(2118..2483)
                     /gene="rplN"
                     /locus_tag="PKJ43_00590"
     CDS             complement(2118..2483)
                     /gene="rplN"
                     /locus_tag="PKJ43_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNZ71082.1"
                     /translation="MIQQESRCTVADNSGAKTALVIRVLGGTGKRYASLGDMVVVTVK
                     SAIASGNLKKGTVSKAVVVRTKKEVRRADGSYIRFDDNAIVLLNNAGEIRGTRIFGPV
                     ARELRDQYMKIVSLAPEVL"
     gene            complement(2486..2749)
                     /gene="rpsQ"
                     /locus_tag="PKJ43_00595"
     CDS             complement(2486..2749)
                     /gene="rpsQ"
                     /locus_tag="PKJ43_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNZ71083.1"
                     /translation="MEENSRNLRKERIGVVVSNKMDKTIVVAEKRKVMHPIYGKFVNR
                     TSKFYAHDEKNDCNVGDTVKIMETRPLSKTKGWRLVEIIERAK"
     gene            complement(2763..2963)
                     /gene="rpmC"
                     /locus_tag="PKJ43_00600"
     CDS             complement(2763..2963)
                     /gene="rpmC"
                     /locus_tag="PKJ43_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNZ71084.1"
                     /translation="MKISEIKELTTKEIAERIETEKEKLTRMKLNHAVSPLENPMVLK
                     DIRKDIARLNTELRARQLTENK"
     gene            complement(2999..3430)
                     /gene="rplP"
                     /locus_tag="PKJ43_00605"
     CDS             complement(2999..3430)
                     /gene="rplP"
                     /locus_tag="PKJ43_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNZ71085.1"
                     /translation="MLQPKKTKFRRVQKGKMKGEAQRGNQLAFGSFGIKALESAWITG
                     RQIEAARVAVTRHMQRQGQIWIRIFPDKPITKKPAEVRMGKGKGAPEAFVAPVTPGRI
                     LIEAEGVPFELAKEALRLAAQKLPITTKFVVRRDYAETASE"
     gene            complement(3455..4177)
                     /gene="rpsC"
                     /locus_tag="PKJ43_00610"
     CDS             complement(3455..4177)
                     /gene="rpsC"
                     /locus_tag="PKJ43_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNZ71086.1"
                     /translation="MGQKTNPIANRLGIIRGWDSNWFGGNDYGDKLVEDYKIRKYLNA
                     RLAKASVSKIIIERTLKLITITVHTSRPGIIIGKGGQEVDKLKEELKKITSKEVQINI
                     FEIKRPELDAQIVASNIARQVEGKIAYRRATKMAIASTMRMGAEGIKVLVSGRLNGAE
                     MARSEMYKDGRTPLHTLRADIDYALAEALTKTGLIGVKVWICKGEVYGKRDLSPNVGM
                     KSNSGARPGAAPKGGNRKNKKK"
     gene            complement(4191..4598)
                     /gene="rplV"
                     /locus_tag="PKJ43_00615"
     CDS             complement(4191..4598)
                     /gene="rplV"
                     /locus_tag="PKJ43_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNZ71087.1"
                     /translation="MGARKRNIANKRKEEKKDQYFAVLRNNPTSPRKMRLVADMVRGM
                     DVNKALDVLKYSSKEASGKVEKLLLSAIANWQAKNENARLEESNLIVSRIFVDSGRML
                     KRIMPAPQGRAHRIRKRSNHVTIYLDKKNPVTE"
     gene            complement(4633..4899)
                     /gene="rpsS"
                     /locus_tag="PKJ43_00620"
     CDS             complement(4633..4899)
                     /gene="rpsS"
                     /locus_tag="PKJ43_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNZ71088.1"
                     /translation="MSRSLKKGPFIAFKLEKKILAMNESGKKSVVKTWARASMISPDM
                     VGHTIAVHNGNKFIPVYVTENMVGHKLGEFAPTRTYRGHAGNRK"
     gene            complement(4922..5743)
                     /gene="rplB"
                     /locus_tag="PKJ43_00625"
     CDS             complement(4922..5743)
                     /gene="rplB"
                     /locus_tag="PKJ43_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016779059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNZ71089.1"
                     /translation="MAVRKLKPVTPGQRHKIIGAFDTITESTPERSLLEPIKKSGGRN
                     SSGRMTMRYIGGGHKRKYRIIDFKRDKNDVEALVKSIQYDPNRSARIALLEYTDGEKR
                     YIIAPVGLKVGQTVVSGDSVAPEIGNALPLAKIPLGTIVHNIELRPGQGGVMARSAGT
                     YAQLLSREGKYAILKLPSGESRMVLITCKATVGTVGNAEHNLERSGKAGRSRWLGRRP
                     RNRGVVMNPVDHPMGGGEGRASGGHPRSRKGLLAKGYKTRTPKKESNKYILEKRK"
     gene            complement(5750..6040)
                     /gene="rplW"
                     /locus_tag="PKJ43_00630"
     CDS             complement(5750..6040)
                     /gene="rplW"
                     /locus_tag="PKJ43_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNZ71090.1"
                     /translation="MDVLVRPLVTEKMTAQSEKLNRFGFIVNRRATRDQIKSAVEKLY
                     GVNVEAVNTMIYGPKTKYRYTKSGVQKGKTNAFKKAIVTLAKGESIDFYSNI"
     gene            complement(6051..>6486)
                     /gene="rplD"
                     /locus_tag="PKJ43_00635"
     CDS             complement(6051..>6486)
                     /gene="rplD"
                     /locus_tag="PKJ43_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNZ71091.1"
                     /translation="KQKGTGGARAGSIKAPVFRGGGRVFGPRPRNYDFKLNKKVKQLA
                     RKSALSYKAKENGIIILEDFNFNAPKTKEFVAIQNNLKINDKKSLFVLSEVNKNIYLS
                     SRNLQGVKVVTASDLTTYDILNASSVVFLEGSLDKIEGVFNN"
BASE COUNT         1598 a         1453 c         1196 g         2239 t
ORIGIN      
        1 caacaaactt aatacctttg cctttataag gttcgggcat acggaatgac ctgattttag
       61 ctgcaacttg tccaataagt tgtttgtcgt agcttttcag ggtaatcgta gggttactac
      121 gtttgtcggt aactgcttca actttcactt cagaaggaag ctgtaaaaag atgtggtgtg
      181 aatacccaag tacaagctgt aaaacctgac cttgattttc agctctgtaa ccaacaccaa
      241 ccaattcgag tttaacctca aatccttttg atacaccggt aaccatatta ttcaacaatg
      301 aacggtacaa accgtgcata gccctatggt ttttttcgtc gctaggacga tttacagtaa
      361 gctgatttcc ttcaattttc acatctattt caggattcac cttttgagaa agttcgccca
      421 ggggcccttt tacggtaacg acgttattat cgtcgacttt aacagttacc ccgccaggta
      481 tctcgatggg taattttcct atccttgaca tcgtattttc ctcctaagat taatagacgt
      541 aacacaatac ttcaccgccg acgtttaaat cacgcgcttc tttatcggtg attacgcctt
      601 tcgatgttga tataatagca atgcccaaac cattgagtac acgtggtaca tcttctgaat
      661 tacaatattt acgcaaccca ggcttgctga tgcgttcgag ctttttaatt gcagaatcac
      721 cagtttcggg atggaacttg agggctattt ttatattacc ctgctggttt acaccatctt
      781 cgaacttgta attgagaata tatccttttt ctttaaggat ttttgtgatt tcctttttta
      841 ggtttgaagc aggaacgtcc actactttgt ggcccgcttt gatagcattt cttaacctgg
      901 tcaaataatc tgatattggg tcagtcattt tacttattct tttaaaatta ccaactagct
      961 tttttaacac ccggaataag accttgcgaa gccatctccc taaaattgat acggctgata
     1021 ccgaattgcc tcatataccc ttttgaacgg ccagtaagtt tacaacgatt gtgcaaacgt
     1081 attggcattg agttttttgg caacttctgt aatccgatat aatcgccatt agcttttaat
     1141 tcttcccttt tagctgcata cttttgaata agttttgcac gcttaacttc acgggctttc
     1201 atcgattcct tagccatatc ttaatccttt ttttcgtttt taaacggtaa cccaaattca
     1261 cgcaacaaag cgtatccttc ttcatcggtt tttgctgtag tcacaaaagt gatgttcata
     1321 ccggtaagac ggttaacttt gtcgagaaca atctcgggaa aaatgatttg ctcggtaatt
     1381 ccgagggtgt aattcccgcg tccgtcgagt ttgccttcaa ttcctttgaa gtcgcggata
     1441 cgaggtaaag caactacgat caaacgttca aggaactcgt acattctttg cctgcgcaaa
     1501 gtcacacgaa cgccaatagg cattttcttc ctgagtttga agttagagat gtctttcctt
     1561 gattttgtct gaacacattt ttgaccagcg atgttggtca tttcttcagt agccgcttca
     1621 ataattttct tatcggctgt tgctgcgcct aaaccttggt taagaacaat cttttctatg
     1681 cgaggtacct gcatgatact tttgtatccg aactctttca tgagtgcagg aactaccttt
     1741 tctgtgtatt ttttctgtag tgaaggtaca aattccatta cttaatctcc tctcctgatt
     1801 tttttgagta acgaactaaa tttccgtcgg catccatacg ccggcctacg cgggtaggtt
     1861 ttccggtttt gggatcagcc accataaggt tggagatgtg gatagatgct tccttttcca
     1921 aaatcccccc ttgaggtgct tttgaattcg gtttggtgtg ttttttcacc atatttacac
     1981 cttccacaat ggcacggtcg ttatcccggt ttacttcgag taccttacct ttttgccctt
     2041 ttgagttgcc tgtaacaact acaacggtat cgcctttttt aatttttaat tttttctgca
     2101 ttgctcttga ttttcattta taatacttca ggagcaagag aaacgatctt catgtattga
     2161 tcacgcaatt cacgggccac tgggccaaag atgcgtgttc cacgaatttc tcctgcatta
     2221 tttaaaagga caattgcgtt gtcgtcgaaa cgaatgtaag aaccatcggc cctgcgcact
     2281 tctttctttg tgcgcacaac cactgccttt gaaacggtac ctttctttag gttacctgaa
     2341 gctatagcgc ttttaacggt cacaacaacc atgtcgccta acgatgcata acgtttccca
     2401 gtacctccaa gtactctgat taccaatgca gtcttagcgc cactattgtc ggccacagta
     2461 catctacttt cttgctgtat catggttact tagctctttc aatgatttct actagtctcc
     2521 atccttttgt tttacttaaa ggacgggttt ccataatttt aactgtatcg cctacgttgc
     2581 aatcattttt ttcatcgtgt gcgtaaaatt ttgaagtcct gtttacgaat tttccgtaaa
     2641 tagggtgcat cactttacgt ttttctgcaa cgacaatggt tttatccatt ttattgctaa
     2701 caacgacacc gatacgttct ttcctgaggt ttcttgaatt ctcttccatt ttacttgaat
     2761 aattacttgt tctcagttaa ctgacgtgca cgcaattcgg tattaagcct tgcgatgtct
     2821 ttccttatat ccttcaaaac catagggttt tcaagaggag aaactgcatg gtttaacttc
     2881 atgcgagtaa gtttttcttt ctccgtttcg atgcgttcag cgatctcttt tgttgttaac
     2941 tctttaattt ccgaaatttt catcagacta ctattttttt aatgaactga tgtttaaatt
     3001 attcgctggc tgtttcagca taatcacgac gaactacaaa ttttgtagta atcggcagtt
     3061 tttgagctgc caatcggagt gcttctttag ccaattcgaa aggaacacct tcggcttcga
     3121 taagaattct gcccggtgtc acgggtgcaa cgaaagcttc tggagcacct ttacctttac
     3181 ccatacgaac ctctgcaggc ttcttagtta tgggtttgtc gggaaagata cgtatccaaa
     3241 tttgtccttg acgttgcatg tgcctggtaa ctgcgacacg agctgcttcg atttgacggc
     3301 ctgtgatcca agccgattcg agtgctttaa ttccgaacga tccaaatgca agttgattcc
     3361 cgcgctgggc ttcacctttc atttttcctt tctgaactct tctaaatttt gttttcttag
     3421 gttgtaacat ccttaataag tatttaaggg ttaattactt ttttttgttc ttacggtttc
     3481 cgcctttagg ggcagcaccg ggtctagcac cactgttgct tttcatgcct acatttggtg
     3541 aaaggtcgcg tttaccataa acttcacctt tgcagatcca tactttcact cctataagcc
     3601 cagtcttggt aagagcctcg gcaagagcat aatcaatgtc agctctaaga gtgtgcagtg
     3661 gggtacggcc atctttgtac atttcagagc gagccatttc agcaccattg agccttccgg
     3721 aaactagcac cttgattcct tcagcaccca tacgcatggt tgatgcgata gccatttttg
     3781 ttgctcttct gtacgcgatt ttgccttcaa cttggcgagc aatgttgctt gccactatct
     3841 gggcatccaa ttctggacgc ttgatttcga agatgttgat ctgaacttct ttagaagtaa
     3901 ttttctttaa ttcttctttc agtttatcaa cttcctgtcc gcctttaccg ataataatac
     3961 caggccttga ggtgtgaaca gtgatagtga taagctttaa tgtgcgttcg attataattt
     4021 ttgaaacgct ggcttttgcc aaacgggcgt tcaaatattt acgaatttta taatcttcaa
     4081 cgagcttatc gccgtaatcg ttcccaccaa accagttgga atcccatcct ctgatgattc
     4141 ccaaacgatt tgcaatcgga ttagttttct gtcccatgta ttactattat ttattcggtt
     4201 acaggatttt tcttatccaa atatattgtc acatgattcg agcgtttgcg aatacggtgt
     4261 gcccttccct gaggagctgg cattattcgt ttcagcatac ggcctgaatc aacaaaaatc
     4321 cttgaaacaa taagattgct ttcttcaagt cgtgcatttt cgtttttagc ttgccaattg
     4381 gctatagctg aaagcaaaag tttttcgact ttccctgaag cttcttttga agaatatttc
     4441 aatacatcga gagctttgtt cacatccata cctctcacca tatcggctac cagccgcatt
     4501 ttgcggggcg aagtaggatt attgcgaagc acagcaaagt attgatcctt tttttcttct
     4561 ttacgtttgt ttgctatatt tctttttctt gcacccattg tctttctttt aaatgattta
     4621 acaattcacg ccttacttac ggtttccggc gtgtcccctg tatgtacggg ttggcgcgaa
     4681 ttcacccaat ttatgtccta ccatattttc ggttacatat actggaatga atttattgcc
     4741 attatgaaca gcaattgtgt gccccaccat atcaggagaa atcattgatg cacgggccca
     4801 tgttttaaca acgctctttt taccgctttc attcatagcc aagattttct tctcaagctt
     4861 gaaggcaata aaaggccctt ttttcaatga acgactcata gttacttacc ttttacaatt
     4921 attacttacg tttttctaat atgtacttgt tggattcttt ctttggtgta cgggttttgt
     4981 atcccttagc caacagaccc ttacgtgaac gtgggtgtcc tccggatgca cgaccttcac
     5041 caccacccat tgggtgatcg actgggttca ttacaacacc acggttacgt ggacgacgac
     5101 ccaaccaacg tgagcgaccg gctttacccg aacgttcaag gttgtgttca gcatttccaa
     5161 cagtacctac agttgctttg caggttatga gtaccatgcg tgattctcct gaaggtaact
     5221 tcagaatggc atactttcct tcgcgggaaa gaagctgtgc ataggttccg gcacttcgag
     5281 ccattacacc tccctgaccg ggacggagct caatattatg gacaatggta ccaagtggta
     5341 tcttagcaag cggaagggca ttgcctattt ccggcgcaac actatcgccc gatacaacgg
     5401 tttgacctac tttcaaacct accggtgcaa taatgtacct tttttctcca tcggtatatt
     5461 ccaacaacgc aatgcgtgca ctgcggtttg gatcatactg aatagatttt actaaggctt
     5521 ctacatcgtt tttatcgcgt ttgaaatcga taatacgata tttgcgcttg tgcccgccac
     5581 ctatatacct cattgtcatg cgaccggagc tatttctacc tccgctcttt ttaatgggct
     5641 ccaacaacga tctttcaggt gttgactctg taattgtgtc aaaagcgcca attattttgt
     5701 gcctttgacc aggtgttaca ggttttaatt tacgtactgc catattatat taaatattgc
     5761 tatagaaatc aattgattcg cctttagcca aagttactat ggctttttta aaggcgtttg
     5821 tttttccttt ctgaactcca gatttggtat aacgatattt ggtttttggg ccatatatca
     5881 ttgtattcac tgcttcaacg tttaccccgt agagtttttc aactgccgat ttaatttgat
     5941 cgcgggttgc acggcggtta acaatgaagc caaaacggtt cagtttttcc gactgagccg
     6001 tcatcttttc tgtgactaat ggtcttacta aaacgtccat ctttcttcaa ttaattgtta
     6061 aacacacctt cgattttatc gagtgaacct tctaaaaata ctacagaaga agcattcaaa
     6121 atgtcataag ttgttaaatc tgaagcagtt acaactttaa ccccctgtaa atttctggag
     6181 gacaaatata tatttttatt tacttctgat aaaacgaata acgacttttt atcattaatt
     6241 ttcagattat tctgtattgc aacaaattct ttggtctttg gtgcattgaa attgaaatct
     6301 tcgagaatga taatgccatt ttcttttgct ttataagaaa gggccgattt gcgagcaagc
     6361 tgttttactt tcttgttcaa tttgaaatcg taatttcttg gacgtggacc aaaaacccgg
     6421 cccccaccac gaaatacagg tgctttgatg ctaccagcac gagcgcctcc tgttcctttc
     6481 tgtttt
//
LOCUS       DAOPUW010000017         6441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_11960_length_6441_cov_3.559035, whole genome shotgun sequence.
ACCESSION   DAOPUW010000017 DAOPUW010000000
VERSION     DAOPUW010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11960_length_6441_cov_3.559035"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1703)
                     /locus_tag="PKJ43_00640"
     CDS             complement(<1..1703)
                     /locus_tag="PKJ43_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HNZ71092.1"
                     /translation="MNTDELNTSIPEETPKTDEHREGRHELKSLSGMYQSWFLDYASY
                     VILERAVPAMEDGFKPVQRRIMHAMKEMDDGRFNKVANIIGQTMQYHPHGDASIGDAL
                     VQLGQKDLLIDCEGNWGNILTGDGAAAPRYIEARLSKFALDVVFNPKTTEWQLSYDGR
                     KKEPVTLPVKFPLLLAQGVEGIAVGLSSKTLPHNFNELIDASIAYLRGEDFELYPDFP
                     TGGLIDVSKYNDGLRGGSVRVRAKIEKIDSKTLAITEVPFGRTTSSLIESIIKANEKG
                     KIKIKKIDDNTAANVEILIQLVPGMSSDKTIDALYAFTDCEIPISPNICVIENDKPRF
                     ITVTELLKHSTDQTVELLRLELEIKKKELEEMWHFASLEKIFIEERIYKDKEFEESKN
                     MDEAVAHIDMRLTPWKPKFKREITREDILKLMEIKMGRILKFNKEKADENLLAIEDEI
                     AGIIKNIEHIIDYAIAYFERIKAKYGANRHRKTEIRSFENIVASKVVVANEKLYVDYE
                     EGFMGTSLKKAEYVCDCSDMDDVIIFRKDGTYFVSKVSEKAFIGKNIQYLNVFKKDDT
                     RT"
     gene            complement(1730..2395)
                     /locus_tag="PKJ43_00645"
     CDS             complement(1730..2395)
                     /locus_tag="PKJ43_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNZ71093.1"
                     /translation="MHYNSNDDRKDDSCLFITEGDSASGSITKSRDVNTQAVFSLRGK
                     PLNSFGLTKKIVYENEEFNLLQAALNIEEGLDDLRYNKVIIATDADVDGMHIRLLLLT
                     FFLQFFPELIRKGHVYILQTPLFRVRNKKETHYCYSDEERLAAIKKCGANAEITRFKG
                     LGEISPDEFKGFIGEDIRLEPVTLHKGDAIKQILEYYMGKNTPERQEFIIENLRVEVE
                     EEL"
     gene            complement(2486..3493)
                     /locus_tag="PKJ43_00650"
                     /pseudo
     CDS             complement(2486..3493)
                     /locus_tag="PKJ43_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012430.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            4096..4779
                     /locus_tag="PKJ43_00655"
     CDS             4096..4779
                     /locus_tag="PKJ43_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HNZ71094.1"
                     /translation="MEDILYKYMSQRFKDKIPNEEGSSLGPVITISRAAGCSTSKLVE
                     ELTFALNHNGLDDKWEIINKDILRHSAELLNLQPEKVESIFKMKKRKLFDEVVQTFIS
                     SDYHLEKKMIKTVTKVIHQFGAEGHKIIVGRAANIICADIPRALHIRIDAPIQWQIEH
                     IEKKKNFTKEEALSYITLTEQNRENFRKTIKGSDQLNVYDLVINQATFNTVEIISIIT
                     NALSLKNIL"
     gene            complement(4851..5192)
                     /locus_tag="PKJ43_00660"
     CDS             complement(4851..5192)
                     /locus_tag="PKJ43_00660"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNZ71095.1"
                     /translation="MSKISKELVGASAIPIILSILDKSESYGYEIMQSVKELSGNRIE
                     WKEGSLYPVLHKLEKNGLIKSLWKMAENGRHRKYYAINKKGRKALQDEIENWTLVHAM
                     LSNLWKDLTPV"
     gene            5503..5760
                     /locus_tag="PKJ43_00665"
     CDS             5503..5760
                     /locus_tag="PKJ43_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71096.1"
                     /translation="MLPRFLLADNSQEYPETIFIVHNEYPRFIVESDIDDFGANQVIH
                     WIDDEPSNETLIEQLLNDAESFMEEEFDNQENLYDEEFGIN"
     gene            complement(5757..>6441)
                     /locus_tag="PKJ43_00670"
     CDS             complement(5757..>6441)
                     /locus_tag="PKJ43_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNZ71097.1"
                     /translation="HVNTASKHFLNYISGLGPQLAQNIVDFRRENGNFESRKQLMKVP
                     RMGAKAFEQSAGFLRIHQAINPLDNSAVHPESYHIVERIATDLGCNVKTLIGNEELIR
                     KIDVRKYVTASIGEPTLNDIVKELSKPGLDPREKISAFEFDENLKTMEDLKIGMTVPG
                     IITNITRFGAFVDIGIKENGLVHISEMTDKYIDDPSKVVKLNQHVRVKIIDLDMVRKR
                     IQLSLRQAD"
BASE COUNT         1890 a         1401 c         1162 g         1988 t
ORIGIN      
        1 gtacgggtat cgtctttttt gaaaacattt aagtattgga tatttttccc aatgaaagct
       61 ttttccgaca ctttggaaac aaaataagtc ccgtcttttc tgaaaatgat aacatcatcc
      121 atatcagaac aatcgcaaac atattcagct tttttcaaac ttgtgcccat gaacccttct
      181 tcatagtcga cataaagttt ttcgttggca acaactactt tgctagccac aatattttcg
      241 aaacttctga tttcggtttt ccgatgcctg ttggcaccat acttggcctt aatccgctcg
      301 aaataagcga ttgcgtagtc aataatgtgt tcaatatttt ttattatccc ggcaatttca
      361 tcctcgatag ccagcaagtt ttcatcggct ttttctttgt tgaacttaag gattcgtccc
      421 attttaattt ccataagctt aagaatgtcc tcgcgggtta tttcacgctt aaacttaggc
      481 ttccatgggg tgaggcgcat atcgatatga gctacagcct catccatgtt tttgctttct
      541 tcgaactcct tgtctttgta gattcgttct tcgatgaaaa tcttttcgag cgaggcaaaa
      601 tgccacatct cttcaagttc tttctttttg atttccaact caaggcgaag caattcaacc
      661 gtttggtcgg tactgtgttt gagcagttcg gtcacggtaa taaaccgtgg tttatcgttc
      721 tcgattacac aaatgtttgg cgatattggt atttcgcaat cggtaaaagc atagagtgca
      781 tcgatagttt tatcgctcga catgccagga accaattgta tgagtatctc aacattagcc
      841 gcagtgttat cgtcgatttt cttaattttt atttttccct tttcgttggc ttttataatc
      901 gattcgatga gcgatgacgt agtcctacca aaaggcacct ctgtgattgc caaagttttg
      961 ctgtcaattt tttcgatttt tgccctcacc cgaaccgaac cgccacgcag gccgtcgtta
     1021 tatttcgaca catcaataag ccctcccgtg ggaaaatcag gataaagttc gaaatcttcg
     1081 ccacgcaggt atgcaatgga ggcatcgatc aattcattaa aattgtgcgg tagtgtttta
     1141 gaagaaaggc cgacagcaat tccctcaacg ccttgtgcca acaaaagcgg gaactttacc
     1201 gggagggtaa caggctcttt cttgcgacca tcgtatgaaa gttgccactc tgttgtttta
     1261 ggattaaaaa ccacatcgag ggcaaatttc gaaagccttg cctcgatata cctcggtgcc
     1321 gctgccccgt cgcctgttaa gatgttaccc cagtttccct cacagtcgat tagcaaatct
     1381 ttttgcccca actgcactaa cgcatcgccg attgaagcat cgccgtgagg atggtactgc
     1441 atggtttgcc ctataatatt ggccaccttg ttgaaacgcc cgtcatccat ctctttcatg
     1501 gcgtgcataa tgcgccgttg aacgggttta aaaccgtctt ccatggccgg aacagctcgt
     1561 tcaagaatta cgtacgaagc gtaatcgaga aaccatgact gatacatgcc cgatagagat
     1621 ttcaattcgt gccgtccttc acgatgctcg tcagttttcg gcgtttcttc gggtattgat
     1681 gtatttaatt cgtcggtatt catccttttc tttccttggt caattaatat tacagttcct
     1741 cttctacctc aacacgcagg ttttcaatga taaactcttg tcgttcaggg gtatttttcc
     1801 ccatataata ctcgaggatc tgctttattg catcgccttt atgtaacgta acaggctcta
     1861 atctaatatc ttcaccaata aaacctttga attcgtcggg agaaatttca ccaagtcctt
     1921 taaaacgggt tatctcggca ttagccccgc acttttttat ggcagcaagg cgttcttcat
     1981 cggagtagca ataatgggtt tcctttttat tacgtaccct gaacaaaggt gtctgcaaaa
     2041 tatatacatg gccttttctg attagctctg gaaaaaactg aagaaagaat gtaagcaaca
     2101 acaggcggat gtgcattccg tcaacatcag catcggtagc aataatcact ttattgtacc
     2161 gtaaatcgtc gagcccctct tcaatgttaa gagctgcttg caaaaggtta aattcctcgt
     2221 tttcgtaaac aatttttttt gtcaatccga acgagttgag cggctttccc ctcaaactaa
     2281 aaacggcttg cgtattcacg tcacggctct ttgtaatcga cccgcttgct gaatcacctt
     2341 cggtaatgaa aaggcagcta tcatcctttc gatcatcatt tgaattgtaa tgcatccggc
     2401 aatcgcgaag tttgcggttg tgaaggttta ctttctttgc ccgttgcttg gcaagttgct
     2461 tgattccgga gattgcttta cgttcgcgct ccgattcctg gatacgacgc aacatagctt
     2521 cagcaatctc gctgtttttg tgcaggaagt tatcgagttc tttttgcaca aaattggcca
     2581 caaagtttcg tacagtggga ccatcggggc caatatcctt cgaaccaagt ttggtcttgg
     2641 tttgtgattc gaataccggt tcttctactt ttatgcttac agcggcaata atcgatgctc
     2701 ggatgtcaga tgcatcaaaa tcttatttat aaaaatcgcg gagagttttc acaatagctt
     2761 ccctgaaggc cattaaatgc gtacctccct gggttgtatg ttgcccgttg acaaatgagt
     2821 aataatcttc accatactgg tttccatggg tcaacgcgat ttcaatatct tcgcctttga
     2881 gatggatgac cggataaatt gggtcgctat ccatgttttc gttcagcaaa tcgagcaagc
     2941 cgttacgcga aagaaaacgt tcgccattaa attcaatcgt taatccagca ttcagaaagg
     3001 tataattctt catcatcgcg gcaacgtagt ccttgatgta gtgatagttt ctgaaaatgg
     3061 tttcgtcggg tttaaatgca acataagtgc cattgccctc gtcgcttggt tttacctttt
     3121 cttcacgggt aataatcccc ttcgagaatt cgacttcctt cacctcgcca tcacggaagg
     3181 ctttcaacac aaattgcgac gacagagcat tcaccgcttt gatacccaca ccattaagcc
     3241 caaccgactt tttaaatgct ttagaatcgt acttggcccc ggtattcatt ttggaagtta
     3301 catcgacaac ctttccaagg ggaattcccc gtccatagtc acgaattgaa acttgctcat
     3361 cggaaacctt aacctcaatt ttgcggccat tgcccatcat aaactcgtca atcgaattgt
     3421 cgatgacctc tttaagcaag acataaatac catcgtcaga ttgagaacca tctcccaatt
     3481 taccgatgta catcccggga cgtttccgta tatgctcttg ccaatcaagc gttttaattg
     3541 agtcttcagt atattgtacc tgctctgtca ttagatttta ttcaaatttc cacaaatata
     3601 ccaaaaacca ttttccatgc cgatttgaag ttatcaacaa ttctccaccc ctaatagtta
     3661 ataacttgtt gcgtttaaat gattttgttc gcttttgcga ggatctatat cattgaatat
     3721 tttgaattag tactagcgaa gaatttcaac gtgcgaacac taggaacaaa aaacactagt
     3781 atttttaaaa acaaatagca ttcattgtta ctggcttact ttttcagaca cccgtagcat
     3841 taaattattc gcaaaacttg agtacaagat ttgcttcagg cagatcaaaa tgggaaaaca
     3901 aattctgttc ggttccagaa aagataatta aacaagctga cactgcggtt attcgacata
     3961 catgaacaaa agaatctttg taagtcgatt attttcatta ggaataaaaa tgtgcttatc
     4021 aattggaaaa catgactcat acaatgtttt ttttgaaaca aaatagtaaa ttgaataaaa
     4081 ataacaaaaa gagtcatgga agatatttta tataaataca tgagccagcg cttcaaagac
     4141 aaaatcccca atgaagaagg gtcatcgctg ggaccggtaa tcacaatttc gcgtgcagcc
     4201 ggctgttcaa caagcaaact tgttgaagaa cttacatttg cactaaacca taacggacta
     4261 gatgataaat gggaaattat caataaagat attcttcgcc attcagcaga gttgctcaac
     4321 ctgcaacccg aaaaggtaga aagcattttc aaaatgaaaa agcgaaagct ttttgatgaa
     4381 gttgttcaaa cattcatctc gtcagattat catctggaaa aaaagatgat caaaacagta
     4441 accaaggtaa tccaccaatt tggagctgaa ggccataaaa taatcgtagg cagggcagcc
     4501 aatataattt gtgccgatat tccacgagct ttacacataa gaattgacgc tccaatacaa
     4561 tggcaaatag aacatatcga aaaaaagaaa aatttcacaa aggaagaggc actcagttac
     4621 attaccctta ccgaacaaaa ccgtgaaaat ttcagaaaaa caatcaaagg cagtgatcaa
     4681 ctaaacgttt atgatttggt aattaatcag gcaactttca atacggtcga aattatatcc
     4741 atcataacca acgccttgag cttgaaaaac attttgtaat agtacaaaaa aagaggctat
     4801 aattattggt acagcctctt ttcatttcag taacaaacta aaaagaatta ctagaccgga
     4861 gtaagatctt tccataggtt tgatagcata gcgtgtacca aagtccagtt ctcaatttca
     4921 tcctgcaacg ctttcctccc cttcttattg attgcataat acttgcggtg acgtccattt
     4981 tctgccattt tccacaacga tttaattaaa ccgttctttt caagtttgtg tagaaccggg
     5041 taaaggctac cttccttcca ttcaatccgg tttcccgaca attctttcac gctttgcatg
     5101 atttcgtagc cgtagctttc gcttttgtcg agaatagaaa gaatgatagg aatagccgat
     5161 gcaccaacta attcctttga aattttactc ataagctatt cttttattaa acaaagtaaa
     5221 aataaaaata atttcatttt aatcaaataa attaagcatc cacagatatt taataacagc
     5281 aaattcacgt agattgttta tcatgcaaca tagataagtt gcacattaaa aagccaatgt
     5341 attgatcgtc tataagttct ccaaaagaat tttgcatggt aaccatgcct ctaaaataaa
     5401 aatcacgtta tgtaaaatta catctcattc aaaacaagca attcaatctt tattaccgaa
     5461 ctagtgaaaa aataatatac atttgcaaaa aaagcaataa ccatgttacc aagattttta
     5521 ctagcagaca attcccaaga gtatccagaa accattttca ttgtacataa cgaatatcct
     5581 cgtttcattg ttgaaagcga tatcgacgat tttggagcca accaagtaat ccattggatt
     5641 gacgatgagc caagcaacga aactcttatc gaacaacttc tcaacgatgc cgagagcttc
     5701 atggaagaag aattcgacaa tcaggagaac ttgtacgacg aggagtttgg cataaactaa
     5761 tctgcctgtc gcaacgaaag ctggatccgc tttcgtacca tatccagatc aattatctta
     5821 acccttacat gctggttgag cttaacaact ttggaagggt catcgatgta tttgtcggtc
     5881 atttctgata tgtgaactag tccgttttct tttatcccga tgtcgacaaa agcaccgaaa
     5941 cgggtaatat ttgtaatgat tccaggtact gtcattccaa ttttcaggtc ttccattgtc
     6001 ttcaggtttt catcaaattc gaatgcactg attttctctc gcggatcgag tcctggtttt
     6061 gacaattctt tcacgatatc gttcaaggtt ggctcaccaa tactggctgt aacatacttt
     6121 cttacatcaa ttttccgaat cagttcttca ttcccgataa gagtctttac gttgcacccc
     6181 agatcagtag caattctttc tacaatgtga taagattctg ggtgaactgc cgaattatcg
     6241 agtgggttta ttgcctggtg aatacgcaaa aaacctgccg attgttcaaa tgcttttgcg
     6301 cccatacgcg gtaccttcat tagctgtttg cgcgactcaa aattgccgtt ctcacgacga
     6361 aaatcaacaa tattctgagc cagttgcggc cccaaacccg atatataatt caaaaaatgt
     6421 ttacttgcag tatttacatg t
//
LOCUS       DAOPUW010000018         6311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_12301_length_6311_cov_3.558504, whole genome shotgun sequence.
ACCESSION   DAOPUW010000018 DAOPUW010000000
VERSION     DAOPUW010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12301_length_6311_cov_3.558504"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            366..791
                     /gene="ruvX"
                     /locus_tag="PKJ43_00675"
     CDS             366..791
                     /gene="ruvX"
                     /locus_tag="PKJ43_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNZ71098.1"
                     /translation="MGRILAIDYGRKRVGLAVTDPLQIIANKLTTVSSHEIFDFLSNY
                     FSKEKVDCVVVGYPKTLNDQPSESIRYINPFLKQFEKKFPDIKLELADERFTSKIAFQ
                     TMIDAGLKKSQRQDKALVDAVSATIILQFYLEQRRNLNL"
     gene            793..1350
                     /gene="def"
                     /locus_tag="PKJ43_00680"
     CDS             793..1350
                     /gene="def"
                     /locus_tag="PKJ43_00680"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNZ71099.1"
                     /translation="MILPITVYGDPLLRKVAQPIDKNYEKLDELIANMYETMYHADGV
                     GLAAPQIGLSIRLFIVDASPAASEEEPELADFKKVFINPVIIEKSGEPWSFEEGCLSL
                     PNIHEEVLREEEVIISYLDENWEQKTEKYNGYPARIILHEYDHLDGHLFIDYISALRR
                     KLLKRKLAAISKGTAKIAYRVRLPK"
     gene            1520..2719
                     /locus_tag="PKJ43_00685"
     CDS             1520..2719
                     /locus_tag="PKJ43_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ71100.1"
                     /translation="MKKTSEELSAELKDLQQKYFDLEKKYTEEVNSHKATDEELRLRL
                     MFFEGIANSTFDGFLVVDPSGKKILQTQRTIELWKIPQEVVADLSGHKQVAHIMYMTV
                     DPQKFIAEIQYQMEHIEEKRLDEVELIDGTVMERYSSPVFGADGTHYGRIYTFHDITD
                     RKNFEKQLIQLNSDKDRFISILAHDLRSPFNSLLGLTELLSVKLQELPPEEIKTIVTS
                     INNTAQKTFTLLEDTLLWASVQSQKITYSPVFTNIDDIIKEVTEILEPVAQFKNITLE
                     YDSTDEHQASIDIYMFKAILRNLISNAIKFTNQLGRIKISASHIDNNTVVKVTDNGVG
                     MKQEIIDKLFSYSPSNSSKGTDNEAGTGLGLMLCKDFVDKHSGKIWIESTPGKGTTVS
                     FSIPDQP"
     gene            2869..4893
                     /locus_tag="PKJ43_00690"
     CDS             2869..4893
                     /locus_tag="PKJ43_00690"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNZ71101.1"
                     /translation="MKPKLSAFNYFPFLRFSLAFIAGIILEEQFGVGQASFFLFLGLC
                     VLLVIFSILLKKSPSRDYYISIMLVLAFVAVGISYTYSYKRDQFKKDPTLKAMYAGIV
                     QDKAPSTNNRWKYTVGLKCSRTNTNFEMEKEKILLYVSDSISNTKIEPGDEIIFMAQL
                     REISNSKNPGEFDFKKYSQRNGIRYQTYVYEPVTKTGIVQYRVKTIALNLRTKLMKLY
                     REAGIDGDEYAVLGALTLGDTNYISNEVRNSFAASGAMHVLSVSGLHVGIIFIVLNFL
                     FSPMNKKPALKPFKVLFLLLSLWFYAFITGLSPSVLRSASMFSFLVIGDNLNRRTNTY
                     NTLAVSACLLLLINPLTLFNIGFQLSYLAVASIVFFQPKFSALITTKNKASKYLWDLI
                     TVSLAAQLGTTPISILYFHQFPSYFLLSNIIVVPAAAIILYLGMAFFFVSFIPGISHA
                     LAFLLKYTTYLLNHSIQFIENLPGSVIDGIYFTFITSVFLYLLIISISAFFITKKGRN
                     LAFVLCTLVLLQAVGLIKDLESITQRRIIVYNNYSEPLVSIINGKDHYYYSPEKDLEK
                     ISQQLLETASMYYRTQKPVALENNHIVPGKKLQATISYANLKIEICQHQHIEDVKNEN
                     QSIKYDTDEQKVSTDWTKIQKSHSLYKKYIDPMTNEYDLKKYGALIINIK"
     gene            5009..6046
                     /gene="trkA"
                     /locus_tag="PKJ43_00695"
     CDS             5009..6046
                     /gene="trkA"
                     /locus_tag="PKJ43_00695"
                     /inference="COORDINATES: protein motif:HMM:NF007038.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HNZ71102.1"
                     /translation="MKVVIAGAGEVGTHLAKMLSKENHEIVLIDEDSEKLEKISNQVD
                     LLTINGFANSIIDLKEAGISKADLFIAVTPFESRNVLACILAKDLGAKKTLARINNDE
                     YFKKAYKPKFIELGVDEIIYPETLAANEIVASVKQPASRVTHEFSGGKLVLSGIKIRD
                     NATIVNKTLEEIGKFSSSFRVVAITRGDSTIIPYGKDQLMPDDIAYFVSTPAALSELY
                     EKTGKDYFGVKNIMILGGSRIAQKTAEKLSDQFNIKIIDVDKERCQQIADKFSNVLVI
                     NGDGRNLELLKEEGIEKMDAFVAVTGNSETNILTCQLAKKMGVKRTVAEVENIDFISL
                     AEQVGIGSVIN"
     gene            6083..>6311
                     /locus_tag="PKJ43_00700"
     CDS             6083..>6311
                     /locus_tag="PKJ43_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HNZ71103.1"
                     /translation="MSSEISSVKCLSSTDAEAVEVIAHPGSKVTTDLIRNIDFPKEAK
                     IGGIIREDQGIIATGDTQIVAGDKVVIFALPS"
BASE COUNT         2148 a         1198 c         1182 g         1783 t
ORIGIN      
        1 catttttatt agattttcaa caacttatac cagatgcaat tctaaatcag tccgaaccta
       61 ttcagtataa tgccgatgaa tagaaatgtt cagtacaatt gagtgggcga aaattggact
      121 attacatttc ttcaatcggt attactaaaa aaattacaat atgaagaata atccagttat
      181 aacagtaagc gcaacatcgc ttgccttacc ttcgaattgg cctcgaccgg aattattcgc
      241 gacatgtaca aaagcgcata gcaagaagta atggaaaaat cagccgatac gaccatgcgc
      301 aaaatcagtg atttgcagtt ttcagaaaaa gatatttcaa ctatctttgt gccgtttatc
      361 agaaaatggg aagaatctta gcaatcgatt atggaaggaa acgtgtgggg ctggcagtta
      421 ccgacccttt gcaaattatt gccaataagt tgacaactgt gagcagccac gaaatcttcg
      481 attttctaag caactatttt tcaaaggaaa aggttgactg tgtggttgtt ggctatccaa
      541 aaaccttgaa tgaccaacct tctgaatcga ttcgttacat taaccctttt ttaaaacaat
      601 tcgaaaaaaa gttccctgac attaaacttg aactggccga tgaacgtttt acatcgaaaa
      661 tagcgttcca aaccatgatc gatgcaggct tgaaaaaaag tcagcgacaa gacaaagcct
      721 tggtcgatgc ggtgagcgcc accattattt tacaatttta tttggaacaa cgcagaaatt
      781 taaaccttta atatgatttt acctataacc gtatacggag acccgctgct tcgtaaagta
      841 gcacagccca tcgacaaaaa ctacgaaaaa cttgacgaac ttattgccaa catgtatgaa
      901 accatgtatc atgcagatgg tgtaggacta gccgccccac agatcggttt gtcaatcagg
      961 ctgttcattg ttgatgcaag ccctgcagca agcgaagaag aaccagaatt ggccgatttc
     1021 aaaaaggttt tcattaaccc tgttattatt gaaaaatcag gagagccatg gtcgttcgaa
     1081 gaaggttgcc taagcctgcc caatatccat gaagaagtac ttcgcgaaga agaagtgata
     1141 atcagctatt tagacgaaaa ctgggaacaa aaaaccgaaa aatacaacgg ctatccggcg
     1201 cggattatct tacatgaata cgaccaccta gatggccatt tatttatcga ttatatttca
     1261 gccttgcgcc gtaaattgtt gaaaagaaaa ttagctgcca tttccaaggg aacagccaag
     1321 attgcataca gagttcggct tccaaagtaa aattcagcaa gtacaatctt cttaaaaatc
     1381 tacagaaagg gatattgact aattacaaaa catttttgta aattgtgatt tatcagaaat
     1441 ccaagcctta gatatgtcgt tagggatttg attcctgaga aaaatgaagg ttgagttttg
     1501 ttgatttata tgggttgcta tgaaaaagac gagtgaagaa ctcagtgctg aattgaaaga
     1561 cttacagcag aaatatttcg atttagaaaa gaaatacaca gaagaagtaa acagccataa
     1621 agccacagat gaagaactcc gtttacgatt gatgtttttt gaaggcattg ccaattctac
     1681 ttttgacgga ttccttgttg ttgacccaag cgggaaaaag atacttcaaa cacaacgaac
     1741 tattgaatta tggaaaattc cacaggaagt tgtggccgat cttagtggac ataaacaagt
     1801 cgcgcacatt atgtatatga cagttgatcc gcaaaagttt attgcagaaa ttcaatacca
     1861 gatggaacac atcgaagaga aacggcttga tgaggttgaa ctgattgatg gcactgtgat
     1921 ggagcgttat tcatcgccgg tttttggtgc tgatggaact cattatggcc gtatttatac
     1981 ctttcatgac atcaccgatc gtaagaactt cgaaaaacaa ctaatccaac taaattccga
     2041 taaagaccgc tttatctcta ttctggcaca tgatctgcga agtccgttta acagtctgct
     2101 tggacttact gaattgttgt cggtaaaatt gcaggaactt ccacctgaag aaattaaaac
     2161 gattgtaact tcgattaaca atactgccca gaaaacattc actttacttg aagacacgtt
     2221 gttatgggca agtgttcaat cgcaaaagat tacctattcg ccagttttta ccaatattga
     2281 tgatataatc aaagaagtta cagaaatact tgagcctgtt gcacaattca agaatattac
     2341 attagagtac gactcaaccg atgagcacca agccagtatc gacatctaca tgttcaaagc
     2401 aattttaagg aaccttattt cgaatgcgat aaaatttaca aaccaactag gaagaatcaa
     2461 aatatcagcc tctcatatcg ataataatac agttgtaaag gttacagata atggggttgg
     2521 aatgaaacag gaaataattg ataaactctt tagctattct ccatcgaatt cttcaaaagg
     2581 aacagataat gaagcaggga caggccttgg gctaatgctc tgtaaagatt ttgttgataa
     2641 acatagcgga aaaatatgga tagaaagtac accgggaaaa gggacaacag taagtttttc
     2701 aattcctgat caaccataat atatgttgag tggaactacg ttgcattatg atagtgatac
     2761 gccttcactt tctataaaat tacgttgacc ttcagtaaaa atttattttc tatactaagc
     2821 atttagattt tttttgtatt ttgtgtttca attggttata gacccgatat gaaacccaaa
     2881 ctttcggcat ttaactattt cccattcctg aggtttagct tagccttcat agcaggaatt
     2941 attcttgagg aacaatttgg agttggtcag gcaagcttct ttttattcct tggattatgt
     3001 gttctattgg taatcttttc tattctttta aaaaaatcac catcacgaga ctattatatc
     3061 agcattatgc ttgtgctcgc ttttgttgcg gttggcattt catatacata ttcatataaa
     3121 agagaccaat ttaagaaaga ccctacacta aaagccatgt acgcaggtat tgttcaagac
     3181 aaagcgcctt cgacgaacaa ccgatggaaa tatactgtag gcctgaagtg ttcaagaaca
     3241 aacacaaatt tcgaaatgga aaaagagaag atattacttt atgtatcaga ctccatctcg
     3301 aatacaaaaa ttgaaccagg cgacgaaatt attttcatgg cgcaacttcg cgaaatatcg
     3361 aattcaaaaa atccaggaga attcgatttc aaaaaatata gtcaacgtaa tggaatacgc
     3421 tatcaaacct atgtttatga accggttaca aaaacaggca tcgttcagta ccgtgtaaaa
     3481 accattgccc taaacctacg cacaaagcta atgaaactgt acagggaagc cggtatcgat
     3541 ggcgatgaat atgcagtact aggggcactt acgttgggcg atacaaatta tatctcgaac
     3601 gaagtgcgca attcttttgc agcttcagga gccatgcacg tgttatcggt atcaggactg
     3661 catgttggta taatattcat agtacttaac tttctattta gcccaatgaa caaaaaacca
     3721 gcgcttaaac cattcaaagt gctgttttta ctgctttcac tttggtttta tgctttcatt
     3781 acagggttat cgccctcggt cttacgctcg gcaagcatgt tttcgtttct tgtcattggc
     3841 gataatctaa atcgacgaac caacacctac aacacattgg cagtatcggc ctgcctgttg
     3901 ttattaatta atccattaac actattcaat atagggttcc aactctccta tcttgccgta
     3961 gccagtattg tttttttcca gcccaaattc tcggctctca tcactacaaa aaacaaagca
     4021 agcaaatact tgtgggattt gattacagta tcgcttgctg cacaactggg aactacccca
     4081 attagtattc tttattttca tcagttcccg agttattttc ttttgagtaa tattatagta
     4141 gtgcctgctg ccgcaataat cctatattta ggcatggcct ttttctttgt atcgtttatt
     4201 ccgggaataa gccatgctct cgcatttctg ctaaaataca caacctacct gctaaaccac
     4261 agtattcagt ttatcgagaa cctgccaggc tctgtcatcg atggaatata cttcactttc
     4321 attacatcag tatttttata tttgttaatc atttctattt cagcattctt tatcacaaaa
     4381 aaaggcagga acttagcttt tgtactatgc actttagttt tgttgcaagc agttgggttg
     4441 atcaaagacc tcgaaagtat tacccaacga cgaattattg tctacaataa ctattcagaa
     4501 ccactggtaa gtataatcaa tgggaaagac cattattact actcacctga gaaagattta
     4561 gagaaaattt cacagcaact acttgaaacc gcttcaatgt actatcgaac tcaaaaacct
     4621 gtcgcattag aaaataacca cattgttcca ggaaaaaagt tacaggctac gatcagctac
     4681 gcaaatttga aaattgaaat ttgtcaacac caacatatag aagacgtgaa aaatgaaaac
     4741 cagagtataa aatacgatac tgatgaacaa aaagtttcga ctgattggac aaaaattcag
     4801 aaaagtcata gtttatacaa aaaatatatt gatccgatga cgaacgaata cgatttaaaa
     4861 aaatatggag cattgataat caacatcaaa taacttttta aaaaataagg cattaacatt
     4921 aaaataaccc gttgaaaaag gtgacaaaaa actttgatta ttggtttaat aataatttaa
     4981 tttgtgccac ttttaatagc agtaaaatat gaaagttgtt attgccggag caggcgaagt
     5041 aggcacacac ttagccaaaa tgctcagtaa agaaaatcac gaaattgtgc ttattgacga
     5101 ggacagcgag aaactcgaaa aaatctctaa tcaagtagat ctattgacta tcaatggttt
     5161 tgccaactca ataatcgacc taaaagaagc aggtatctct aaagccgatc tgtttattgc
     5221 agtaacccca ttcgagtcga gaaacgtgct ggcctgcatt ttagcaaaag atctgggagc
     5281 aaaaaaaacc ctcgcaagaa tcaacaacga cgaatacttc aaaaaagctt ataaaccgaa
     5341 atttatcgaa ctgggagtag acgaaattat ctatcccgaa acactggcag ccaacgagat
     5401 tgtagcctct gtaaaacaac cggcttcacg cgtaactcac gagttttcgg gcggaaaatt
     5461 ggttttatcg ggcataaaaa tcagggataa tgccacaata gtgaataaaa ccctcgaaga
     5521 aatcgggaaa ttcagttctt cgttcagggt agtagctatc acacggggcg attccacaat
     5581 tatcccttat ggcaaagatc agttaatgcc tgacgatatc gcctactttg tatctacccc
     5641 tgcagcactt agcgaattgt acgaaaaaac cgggaaagat tattttgggg ttaaaaacat
     5701 aatgattctt ggaggcagcc gtattgccca gaaaacagcc gaaaaattaa gtgatcaatt
     5761 caatattaaa attattgatg ttgacaaaga gcgctgccaa caaattgccg acaaattcag
     5821 caatgtactt gtaatcaatg gagatggaag aaaccttgaa cttttaaagg aagaaggcat
     5881 tgaaaagatg gatgcctttg tggctgtaac cggaaactca gaaaccaaca tcctcacttg
     5941 ccagttggcg aaaaaaatgg gggtaaaacg aactgttgcc gaagtagaaa atatcgattt
     6001 cattagtttg gccgaacaag taggcatagg cagtgtaatc aactaaaagt ttattgcagc
     6061 aagttttatt tataggtttt caatgagttc cgaaatatcg agcgtaaaat gcctcagctc
     6121 aaccgatgct gaagctgttg aggtaattgc acaccctggt tcaaaagtaa ctaccgacct
     6181 gatacggaac atcgattttc ccaaagaggc aaaaatcggg ggtattatcc gcgaagatca
     6241 agggattatt gccaccggtg atactcaaat agttgctggc gacaaagtgg tgatttttgc
     6301 attaccttcg g
//
LOCUS       DAOPUW010000019         6201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_12566_length_6201_cov_3.300846, whole genome shotgun sequence.
ACCESSION   DAOPUW010000019 DAOPUW010000000
VERSION     DAOPUW010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12566_length_6201_cov_3.300846"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PKJ43_00705"
     CDS             complement(<1..807)
                     /locus_tag="PKJ43_00705"
                     /inference="COORDINATES: protein motif:HMM:NF013958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HNZ71104.1"
                     /translation="MAKEKSYQEINEKIKKGEAVVLTAEEVAQLAKTCTPEEIVEKVD
                     VVTTATFGAMCSSGVFINFGHADPPIRMEKIRLNGVPAYGGLAAVDTYLGATSVNSNN
                     PEYGGAHVMQELIEGKDIILEAWGKGTDCYPRKHIRTVINKNTINEMILFNPRNAYQN
                     YNVAVNTTSKIKYTYMGTLLPNMRNATYSTAGELSPLINDPEFKTIGIGTRIFIGGTT
                     GYVAWPGTQFHTTKPKDGNGVPVTNAATLAVIGNMKEMSSAFLQAAYYEKY"
     gene            complement(945..1841)
                     /locus_tag="PKJ43_00710"
     CDS             complement(945..1841)
                     /locus_tag="PKJ43_00710"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71105.1"
                     /translation="MLVPIFSYCEIVFDVSQVKEIPRSETEALVSIYESLNGDNWKCK
                     RNWLSSPHPSEWYGVTVINGHVTEISLLNNNLTGSIPDAIKKLADLEILDLFNNKISG
                     HIPEGLCYIETLKKLNLANNNLSGEIPQNIGNLHKLEELILSNNQLEGNIPASLSSLI
                     NIRGISLINNKLTGYLPENIGLLKNLVYFSASNNKLNGQIPPSIGELQSLKSINLAEN
                     NLSGTIPTEIANLKEINRINFSKNQLEGSIPDEITKLENLNYLDFAENNLSGMLPKGI
                     FQMKNLRVLILKNNRLAQPNIY"
     gene            2073..3545
                     /gene="cysS"
                     /locus_tag="PKJ43_00715"
     CDS             2073..3545
                     /gene="cysS"
                     /locus_tag="PKJ43_00715"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNZ71106.1"
                     /translation="MDEQLLIYTTTQKKKVPFSPITPGFVGMYVCGPTVYGHAHLGHA
                     RPAITFDLVFRYLQHIGYRVRYVRNITDVGHLVNDADEGEDKIGKKARLDQLEPMEVV
                     QMYTNSYRLNMAKLNVLPPSIEPTATGHIIEQIELVKTILANGYAYESNGSVYFDVQK
                     YATRFDYGKLSGRRIEDLLCNTRDLDGQDEKRSGLDFALWKKAQPEHIMRWPSPWSEG
                     FPGWHAECTAMGKKYLGEVFDIHGGGMDLMFPHHEAEIAQSCASMGHEAVNYWMHNNM
                     ITINGQKMGKSLGNFITLDELFTGSHPLLEKAYSPMAIRFFILQAHYRSTLDFSNEAL
                     QAAEKGLERLMNATASIAKITPSQHSTVDVDTLRQSCFAAMNDDFNSPIVIANLFDGV
                     RMINQIIEGKATINQADLEKLSELFQLFVFEILGLQQEVNDSADNEMLSGVVDLLLTL
                     RMEAKANKDWVLSDKIRNELTTLGFAIKDLKDGFEWEIKK"
     gene            complement(3619..3855)
                     /locus_tag="PKJ43_00720"
     CDS             complement(3619..3855)
                     /locus_tag="PKJ43_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71107.1"
                     /translation="MNAYDNILTQKIYDLIAPYLGDLMAKGAIRSQCKKIGIDDNKIQ
                     RHDLPALAQNMAKAMNLFVGAENAEMLAARIKTL"
     gene            complement(3859..4662)
                     /locus_tag="PKJ43_00725"
     CDS             complement(3859..4662)
                     /locus_tag="PKJ43_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71108.1"
                     /translation="MKNVRNFWILSGIIVLFNIVAYVFIEGNTLLIISDLLPVVCAAL
                     SIMGVASVINYLKTFDITKKVWMLMLIGLVFNFVAELLYFVFEVIQQRNMDDFFPSYA
                     DPFWIIGFVFFFVSIISMIIYYLRSGMPHGKTQIHLIIIFSTIVLAILVTNFLLLPIA
                     RDPETSLAQKAVSLFYPITDISVVCLSLIVMYIVNQFSNRLFSMPWILIALGYLFFTA
                     SDLIYSYLSWQDDYSAGNFFDIGWNAGYLLIGLAGIYQFNLMKKIKEER"
     gene            4975..>6201
                     /locus_tag="PKJ43_00730"
     CDS             4975..>6201
                     /locus_tag="PKJ43_00730"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: protein motif:HMM:TIGR03542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNZ71109.1"
                     /translation="MASINDNYLKLKAGYLFPEIGRRVKQFTEANPSAKVIRMGIGDV
                     TRPLTPTIIKAFHEGVEEMAHFSSFKGYGPEQGYDFLREAIAEHDYKARGANISADDI
                     FISDGSKCDTGNIQEIFGSENIVALGDPVYPVYADTTVMSGKTGDSDETGYFEGIVYM
                     PCTEENGFVPQLPKEKVDLIFLCFPNNPTGAVATASELKIWVDYALENKCIIFFDAAY
                     QAFITDPAIPRTIYEIEGAEKCAIEFRSFSKTAGFTGTRCAFTVIPEGVMAFDKAGNP
                     HKVKPLWNRRHTTKFNGVSYPVQKAAAAIYTPEGKKETDEVIAYYLENAQIMRDRLHE
                     AGYVVFGGINAPYVWIKTKNDMKSWDFFDKLLHDANVVGTPGSGFGPSGEGYFRFSAF
                     ADRENVIEAMERIKNLK"
BASE COUNT         1826 a         1156 c         1319 g         1900 t
ORIGIN      
        1 atatttttcg taataggctg cctgcagaaa tgcggatgac atttctttca tgttaccaat
       61 aactgcaagg gttgctgcat tagtaacagg aaccccgttt ccatcttttg gttttgtggt
      121 gtggaattgc gttcctggcc aggcaacgta acctgttgtt cccccaataa atatgcgggt
      181 cccaattcca atggttttaa attcgggatc gttgatcaac gggcttagtt ctcctgcagt
      241 tgagtaggtt gcgtttcgca tattaggcag cagggtaccc atgtaggtat attttatttt
      301 cgaggttgta ttaacggcta cattataatt ttggtaggca tttcgcgggt taaacaggat
      361 catttcgttg atcgtattct tgttgattac cgtgcggatg tgtttccggg gatagcagtc
      421 ggttcctttt ccccaagctt caagtataat gtcttttcct tcaattaatt cctgcattac
      481 gtgggcacca ccatattcgg gattattaga atttaccgaa gttgcaccca gataagtatc
      541 aacagcagca agcccaccat aagcaggtac gccattaagc ctgatttttt ccatacggat
      601 gggtgggtcg gcgtggccaa aatttataaa cactccgctt gaacacattg ctccaaaggt
      661 tgctgtggta acaacgtcta ctttctcaac aatttcttca ggtgtgcagg ttttcgccag
      721 ttgggcaact tcttctgccg taagcaccac tgcttcacct ttttttatct tctcgttaat
      781 ctcttgataa gatttttctt tagccatttt ttggaatttt ctgccgttat attatttctg
      841 tccgttaata tcacaaatat atattccgaa tatttgctga ctggatagaa agccggtaaa
      901 attaaaccca atttggaaga atgcaaaata aaatcgaact caggttaata gatattgggt
      961 tgcgctaagc ggttattttt caggataagt actcttagat ttttcatttg aaaaattcct
     1021 ttgggcaaca tcccagaaag gttgttttct gcaaaatcga ggtagttcag gttttccagc
     1081 ttggtgattt cgtcaggtat actgccttcc aattggtttt tgctaaagtt tatcctgttt
     1141 atttctttca ggttggctat ttcagtgggt attgtgcccg acaagttgtt ttcagcgagg
     1201 ttaatcgatt ttaaactttg taattctcct attgaaggtg ggatttgccc attgagtttg
     1261 ttgttcgatg ctgaaaaata aaccaggttt tttagtagac caatgttttc tggtaaatag
     1321 cctgttaatt tgttattgat gagtgatatg ccacgaatat ttatgagcga cgatagcgaa
     1381 gctggtatgt tgccttcaag ttggttgttc gacaggatta attcttcgag tttgtgcaag
     1441 ttcccaatgt tttgcggaat ttctcctgat aaattattgt tggccaggtt aagtttcttt
     1501 agcgtttcga tatagcaaag gccttcaggg atatgaccgc ttattttatt gttgaacaag
     1561 tcgaggattt ctaaatcggc caattttttt atggcgtcgg gaatgctgcc ggttaagttg
     1621 ttgttgagaa gtgatatttc tgttacatgt ccattaataa ctgtaacacc gtaccattct
     1681 gatgggtgtg ggctgctaag ccagttcctt ttgcatttcc aattgtctcc gttaagcgat
     1741 tcgtagatcg ataccaaggc ttcggtttca gaacggggta tttcttttac ctgcgaaaca
     1801 tcgaaaacaa tttcacagta tgaaaaaata ggaaccagca aaaaaaggca gattagagac
     1861 tgacgaaaat gccttatggt caattccatt ttgcttatta taagtgtttt aagaatagtt
     1921 ttacaaatcc tttcgcctct gtcttttcag ccatcaaaaa tattaaatat gtctattcat
     1981 tgtgcatgaa tctctttatt ttctctcttt atttttggca aaacgaaatt caatgcagaa
     2041 ttactaaatt tgcagactat aatatgtttg caatggatga acaactattg atttacacga
     2101 ctacgcagaa aaagaaagtg cctttttctc caataactcc cggttttgtg ggtatgtatg
     2161 tttgtggtcc aacggtttat ggacacgctc acttgggtca tgcccgtcct gcaattactt
     2221 ttgacttggt ttttagatat ttacaacata ttggctaccg ggtcagatat gtaaggaata
     2281 ttaccgatgt agggcatttg gtgaacgatg ccgacgaggg agaagataaa atagggaaga
     2341 aagcacggct agatcagctg gagccaatgg aagttgtcca gatgtatacg aacagctacc
     2401 ggttgaatat ggcgaaattg aatgttctac cgcctagtat tgagcctact gccacaggac
     2461 atatcattga gcagattgaa cttgtgaaaa caatccttgc caatggctat gcttatgaaa
     2521 gcaacgggtc ggtttacttc gatgttcaaa aatatgctac gcgtttcgac tatgggaaac
     2581 tgtcgggacg gagaatagaa gacttacttt gcaacacccg cgacctcgat gggcaagatg
     2641 aaaagcgttc agggcttgat tttgcattat ggaaaaaggc tcaacccgaa catattatgc
     2701 gttggccgtc gccctggagt gaaggttttc ccgggtggca tgccgaatgt actgccatgg
     2761 gtaaaaaata tctgggcgaa gttttcgata ttcatggagg aggtatggat cttatgttcc
     2821 cgcaccatga agccgaaatt gcacagagtt gtgcttcgat ggggcatgag gcggtcaact
     2881 actggatgca caataacatg attaccataa acgggcaaaa aatgggtaag tcgctgggaa
     2941 attttatcac cctcgacgaa ctttttacag gttcacatcc cctgctcgaa aaggcttaca
     3001 gtccaatggc tattcggttt tttatccttc aggcacatta ccgtagcaca ctcgatttct
     3061 cgaatgaagc attgcaagcg gctgaaaaag gattagaacg gcttatgaat gcaactgcat
     3121 cgattgccaa gattacacca tcgcaacatt ctaccgtcga tgttgacaca cttcggcaaa
     3181 gctgttttgc cgcgatgaac gacgatttca acagcccgat tgtaattgcc aaccttttcg
     3241 atggtgtgcg catgattaac cagatcattg aaggtaaagc gaccataaac caagcagatc
     3301 tggagaaact ttcagaacta ttccaactgt ttgtattcga aatccttggt cttcaacaag
     3361 aagtgaatga cagtgccgat aacgaaatgc tttcgggtgt tgttgacctg ttgcttaccc
     3421 taagaatgga agcaaaggct aataaagact gggtattgtc tgataaaatc cgcaacgaac
     3481 tcaccaccct tggttttgca attaaagact tgaaagatgg ttttgagtgg gagataaaaa
     3541 aatagaagac atggtaacaa tgccttctat ttttttaact gactgattat aagtatcaac
     3601 ccaaaacaac tgggctattt aaagggtttt tattctggca gctagcattt ccgcattttc
     3661 ggctccaaca aacaggttca tcgctttggc catgttctgg gctaatgccg gaaggtcgtg
     3721 ccgttgaatc ttattgtcat ctattccaat ttttttacac tgcgagcgaa tcgctccttt
     3781 agccattaaa tcgcccaaat aaggagcaat caaatcgtaa attttttggg taagtatatt
     3841 gtcgtatgca ttcatctttt acctctcttc cttaattttt ttcattaaat taaattgata
     3901 gatcccggct aagccgatca ataaatatcc ggcgttccat ccaatatcga aaaagttgcc
     3961 ggcagaataa tcatcttgcc agcttaagta tgagtaaatg agatcggatg ctgtaaagaa
     4021 caaatagccg agtgcaatta aaatccaagg catggaaaag agcctgttgc taaactggtt
     4081 cacgatatac atcacaatta gagacaagca aacgactgaa atatcggtaa ttggataaaa
     4141 aaggctgact gctttttgcg caagacttgt ttcagggtct cttgcaattg gaaggagcag
     4201 aaaatttgtt accagaatgg caagaacaat agtggagaaa atgatgatca gatgaatttg
     4261 ggttttacca tggggcattc cacttctcaa gtagtaaata atcattgaga taatacttac
     4321 aaagaaaaag acaaatccaa ttatccaaaa gggatcggca tagctgggga agaagtcatc
     4381 catgtttctc tgttggatta cttcgaacac aaagtatagc aattccgcaa caaaattgaa
     4441 aactaagcca ataagcatta gcatccatac ttttttggta atgtcgaaag tcttcaggta
     4501 attaataact gaagcaactc ccatgatcga tagagctgcg catactacag gaagtaagtc
     4561 gctgatgata agcaatgtat ttccttcaat gaaaacatac gcaacaatgt tgaataaaac
     4621 aataattccg gaaagtatcc agaagtttct cacgttcttc atattccttt attaaaaatt
     4681 aatttacgtt atctcttatg atgtattatt gcttccaaca gcgatgctta atgaaccgta
     4741 taagtactga aaagatatgc aaatctttca gtgtcaaatt ctggtaaata aatttaggat
     4801 gataatatgc agaagtcaat agctgttttt aagttttttt aatgaaatct tgatgaataa
     4861 aaattaacaa acagtaaacc attttgccag aaatgagaac atttcaatat tgaaaatcaa
     4921 tttttctatc tttgtggcta attgaatcgt ttataaaaag aaaaaaggaa aagcatggca
     4981 tcaattaacg acaattatct taagcttaaa gcaggatatt tatttccaga aattggacga
     5041 agggttaaac aatttactga agcaaaccct tcagcaaagg taattcgtat gggaatcggc
     5101 gatgtaaccc gtccacttac tcctaccata ataaaagctt ttcacgaagg ggttgaagaa
     5161 atggctcatt tcagttcttt taagggctat gggcctgagc aggggtatga ttttctgcgg
     5221 gaagccattg ccgaacacga ctacaaggcc aggggagcca acattagtgc cgatgatatt
     5281 tttatttcag acggatcgaa atgtgacacc gggaatattc aggaaatatt tggcagtgaa
     5341 aacattgtag cccttggcga tcctgtatat cctgtatatg ccgacacaac agtgatgtcg
     5401 ggtaaaactg gcgactcaga cgaaacggga tattttgagg ggattgtgta tatgccttgc
     5461 accgaggaaa atggttttgt gcctcaattg cctaaagaaa aagtggattt gattttttta
     5521 tgctttccaa ataacccaac aggagctgtg gctactgctt ctgagttgaa aatatgggtc
     5581 gattatgcgt tggaaaacaa atgtattatc tttttcgatg ctgcatatca ggcatttatt
     5641 acagatccgg ctattccacg gacaatttac gaaattgaag gagcagaaaa atgtgcgatt
     5701 gaattccgca gtttttcaaa aacagctgga tttaccggaa cccgttgtgc tttcactgta
     5761 atccccgaag gtgttatggc attcgacaag gcaggaaatc cgcataaagt taaaccgctt
     5821 tggaaccgca ggcatacaac caaatttaac ggtgtttcct acccggtaca aaaagctgct
     5881 gctgcaattt atactcccga agggaaaaaa gaaaccgatg aagtaattgc ctactatctt
     5941 gaaaatgcac aaattatgcg cgataggctt catgaggcag gttatgtggt ttttggagga
     6001 ataaatgcgc catatgtttg gataaaaacc aaaaatgata tgaagtcgtg ggatttcttc
     6061 gataagttac ttcacgatgc caatgttgta ggtacccccg gctcgggctt tggcccttcg
     6121 ggcgaaggct atttcaggtt ttctgcgttt gccgatcgcg aaaatgtaat tgaggcaatg
     6181 gaacgaatta aaaaccttaa a
//
LOCUS       DAOPUW010000020         6117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_12810_length_6117_cov_4.063675, whole genome shotgun sequence.
ACCESSION   DAOPUW010000020 DAOPUW010000000
VERSION     DAOPUW010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12810_length_6117_cov_4.063675"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(469..1176)
                     /locus_tag="PKJ43_00735"
     CDS             complement(469..1176)
                     /locus_tag="PKJ43_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNZ71110.1"
                     /translation="MSYLNEIIQSTEINTATTLFRLGLSVVAGALIGVERESHRQPAG
                     IKTHMLICIGSTLMMLLSIYIPQTFSSFQNGDPGRIAAQVISGIGFIGAGTIMRLGIN
                     VRGLTTAASIWAIAGVGLAIGAGMYIPAFITVALLLIVLIVVEQLERYFFTPLSLKIL
                     TVKSTSAETDKKIRQMLNKYHFRVIEVNPAYSRTDNFGFMYKIGSSNKVGLVELTQLL
                     AEKDQSIVSIEISDPML"
     gene            complement(1259..2923)
                     /gene="recN"
                     /locus_tag="PKJ43_00740"
     CDS             complement(1259..2923)
                     /gene="recN"
                     /locus_tag="PKJ43_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNZ71111.1"
                     /translation="MLSSLSIINYAIIKQLTIDFSSGFCVITGETGAGKSIIMGALSL
                     ILGQRADSATLNNTDEKCIIEGRFTILSTPTLRSFFENNDLDFEIPIVMRREITPTGK
                     SRAFINDTPVQLPLMRELGLMLIDIHSQHSNLELAKRQFQLNVIDWYGNHTELIALYQ
                     NVFSAMKKTESQLNTTLEKAKQSKADLDYYEFQFAQLIEMKLVDGEQEILEQELEMLS
                     HAEEIKSGLDHVHTLLDGEEISTLTQLKESISTLQRFSAYFNQAQQLISRLDSQYLEM
                     RDIALECEMLAEKIDHNPSRLEEVSARLDSLYSLEQKHRVNSVGELISLRNDFDAKIQ
                     ASASFDMELEQLSKQLEKQKTEVGLNATKLHNKRLAVLPAIEKEIVAYLQQLGMPNAQ
                     FSIELNQKEDYTPIGNDEAVFLFTANKGTQPEEINKVASGGELSRLMLAIKTVIARSK
                     ALPAIVFDEIDTGISGEIASRMGVILHSMSEYMQVINITHLPQIAAKGNAHYLVFKTE
                     KDKNVETGMRLLSNEERVVEIAKMLSNNNPTPEAIANARSLLTSAN"
     gene            complement(2925..3863)
                     /locus_tag="PKJ43_00745"
     CDS             complement(2925..3863)
                     /locus_tag="PKJ43_00745"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HNZ71112.1"
                     /translation="MTKINQLFSILFFLLFTTTIVQAQELRCNISVNSNKLTSANKNV
                     FRTMQADLYEFMNNKKWTKHNYTNAERIECSITIQITRQLSSNEYEGTLNIQSKRPVY
                     NSSYKTTILNVQDEYFRFKYEEYQTIEYAETDNKDNLTSVLAYYAYVILGFDYDTFSL
                     NGGTEYFEKARQIVNESQNAVGAKNGWKAFESDFNRYWLVENMLNKSYSSYRECLYRY
                     HRKGLDAMADGVEAGRAEIADCLKLIQKVFRTRSKLYITQVFFDAKSSELVSLFSQSY
                     PDEKNRVIQLLSECDPSNSSRYETIKNSESTQSITN"
     gene            complement(3993..4643)
                     /locus_tag="PKJ43_00750"
     CDS             complement(3993..4643)
                     /locus_tag="PKJ43_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HNZ71113.1"
                     /translation="MNNKRFLVTAGPTHEKIDPVRFIGNYSSGKMGFAIAEELASRGA
                     YVYLIAGPVSVQTKNKNIQRINITSAQEMYEQCVQIFPKADGAVMCAAVADFAPVVSS
                     ATKIKRTSEELTITLKPNPDIAASLGKIKKKNQLLVGFALETNDEVNNATSKLLRKNL
                     DFIILNSLNDKGAGFQADTNKITIIDRNNKTYNFELKRKDKVAVDIVDRMVEEMGL"
     gene            complement(4640..5191)
                     /locus_tag="PKJ43_00755"
     CDS             complement(4640..5191)
                     /locus_tag="PKJ43_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HNZ71114.1"
                     /translation="MKEKHIILGITGSIAAYKAATLLRGLIKEGAEVQVVITPSGKEF
                     ITPVTLSALSGKPVISEFFGANDGTWNSHVDLGLWADLMVIAPATASTIGKMANGIAD
                     NMLITTYLSAKCPIMVAPAMDLDMFAHPSTQKNLETLRTYGNIIIEPSTGELASGLTG
                     KGRMQEPEAIAEEIISFFSEKKN"
     gene            complement(5212..5550)
                     /locus_tag="PKJ43_00760"
     CDS             complement(5212..5550)
                     /locus_tag="PKJ43_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNZ71115.1"
                     /translation="MDYKKTKAANSTISRNIDQLAEETGNIYESVMILAKRSNQIASE
                     LKEELNQKLSEFASYTDNLEEVFENREQIEISKFYERLPKSTLIATQEFIEKGIYHRN
                     PMKDSRKVRF"
     gene            complement(5613..>6117)
                     /gene="bamD"
                     /locus_tag="PKJ43_00765"
     CDS             complement(5613..>6117)
                     /gene="bamD"
                     /locus_tag="PKJ43_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNZ71116.1"
                     /translation="FMVGMCFYKDSPNPRLDQEISQKAIDAFQLFINLYPYSNKVEEA
                     NMRIDELNEKIVYKSYLSAKLYYDMEYYKSAVIALNNSLNDYPSTKYREELKFLLFKS
                     KYLLAVNSVEEKKRERTIDARDEYFTFADEFPQSKYLKEVNRDYKHIQQLLGLKEDET
                     SMVGKNQ"
BASE COUNT         1574 a         1188 c         1300 g         2055 t
ORIGIN      
        1 aaagataaca ctttgcaccc aatttattgt tctcgcagtg aataattcgg gttaagactc
       61 ccgctactta gaacgaactc aattataaac tcttgatctt tatcatcgag cgaaagaaat
      121 aatggaagtg agaaacttcc aatgatcgat gtttggcatg catcacattg caggctgtga
      181 acactgagcc ctgcgtgaca actcgggcat tgtatcggaa gtttcttgtt cattggtaca
      241 tttattttga aacaaatata atcaggaatg aataaaatca aacattaatt aaataaaatt
      301 aaagtaattt ttaatataat gaaataataa aagagtaaat gtgaattttg aatttgtttt
      361 ataccaaagt ctagagctta aattagttct tgatgttttg aaagatggtt gcgaaaactt
      421 gctggaacat ataaaaaaca gcgttaaaag ggaactcttt tttccaggtt aaagcatggg
      481 gtctgatatt tctatcgaca ctattgattg gtctttttcg gcaagcaatt gggttagctc
      541 aactaggcca actttgttcg acgaaccaat tttatacata aacccgaaat tatcggttcg
      601 tgaatacgct gggtttactt caataaccct gaaatgatat ttattaagca tttgtctgat
      661 ctttttgtct gtttcggctg aggtagattt tacggtgagt attttcaggc tgagcggggt
      721 gaagaaatat ctttcaagtt gttctacaac aattaacaca attagcaaca acgcaacggt
      781 aataaatgca gggatataca ttccggctcc tatggcaagg ccaactccgg caatagccca
      841 tatagaagct gctgtagtta aacctctaac gtttattcct agtcgcatta tggttcctgc
      901 accaatgaat ccaataccgg agattacctg tgctgcaata cggccagggt caccattttg
      961 aaacgagctg aatgtttgtg gaatatagat ggaaagtaac atcattaatg tggaaccgat
     1021 acaaatcagc atgtgtgttt taattcctgc cggttgtcgg tgcgattccc tttctacacc
     1081 aattaaagca cctgccacaa cactaaggcc aagccggaat agggtagtgg cggtatttat
     1141 ctcagtcgat tggattattt catttaggta actcattttt gactcgtttt tacgaatgta
     1201 atacaaaaac ccgaaaattg ccaagaaaag tgaggctacc cgctatttac tccggttatt
     1261 agtttgctga tgtaagtaat gagcgggcat tcgcaattgc ttcaggggta gggttattat
     1321 tgctgagcat tttggcaatt tcaactactc gttcttcgtt gctgaggagc ctcatgccgg
     1381 tttcaacgtt tttgtctttt tctgttttga aaacaaggta atgggcattg ccttttgcag
     1441 ctatttgtgg gaggtgggtt atatttatta cctgcatata ttccgacata gaatggagta
     1501 taactcccat tcttgaggca atttcccctg aaatgcctgt gtctatttcg tcgaagacaa
     1561 tggccggaag cgctttcgaa cgggctatta ctgttttaat agccagcata agtctcgaca
     1621 actcgccccc tgaggctact ttgttaatct cttcgggctg agttcctttg ttcgctgtga
     1681 aaaggaatac ggcttcgtcg tttccgatag gcgtgtaatc ttctttttga ttgagctcaa
     1741 tgctgaattg tgcattgggc atgccaagtt gctgtaagta tgcaactatt tctttttcaa
     1801 tagctggtaa aacggctagt cgcttattat gcagtttcgt ggcatttaat cctacttcgg
     1861 ttttctgttt ttcgagttgc tttgacaatt gttcaagctc catatcgaac gaggcagatg
     1921 cctgaatttt cgcgtcgaaa tcattccgaa gcgaaatcag ttcaccaaca ctgttcaccc
     1981 ggtgtttttg ttcaagcgaa tacaaactgt caagccgtgc tgaaacttct tcgaggcgcg
     2041 aagggttatg gtctatcttc tcagccaaca tttcacattc caaagcaata tcgcgcatct
     2101 ccagatattg actatccagt cgggatataa gttgctgtgc ctgattgaaa tatgcactaa
     2161 atcgctgcag cgttgaaata gactctttca gttgtgtaag ggttgatatt tcttctccgt
     2221 cgagcagggt gtgtacatgg tcgaggcccg atttgatttc ttcagcgtga ctgagcattt
     2281 ccaattcctg ttccaatatt tcttgctctc cgtcaacaag tttcatctcg attaactgtg
     2341 caaactggaa ttcgtaatag tcgagatctg cctttgattg ttttgctttt tcgagggtag
     2401 tatttagttg gctttcggtt tttttcattg ccgaaaaaac gttttgatac agggctatta
     2461 actccgtatg gtttccatac cagtcgatta cattgagctg aaattgccgt tttgccagtt
     2521 caaggttcga atgctgcgaa tgtatgtcga tgagcataag ccccaattcc cgcattaggg
     2581 gtaactgtac aggggtgtcg ttgatgaaag ccctcgactt gccggtgggc gtaatttcac
     2641 gccgcattac tatggggatc tcgaaatcga ggtcattatt ttcgaaaaaa gaacgaaggg
     2701 ttggggtgga aagaatggtg aaccggcctt ctatgatgca tttttcatcg gtattgttca
     2761 gcgtggcgct gtcggccctt tgaccaagga tgagagacaa agctcccata atgatcgatt
     2821 ttccagctcc ggtttctcct gtaatcacgc aaaagccaga cgaaaaatcg atggttaatt
     2881 gctttattat tgcgtagttg atgattgaga ggctacttag catatcaatt ggtaatgctt
     2941 tgtgttgatt cgctgttttt tattgtctca tacctcgatg aattcgatgg atcacattct
     3001 gaaagcaatt ggattacacg gttcttctcg tcggggtacg attggctaaa taagctaacc
     3061 aactcgctcg attttgcgtc gaagaatact tgtgtaatgt aaagttttga tcgggtgcgg
     3121 aaaacctttt gaatgagttt caggcaatct gcaatttctg cccgaccggc ttctactccg
     3181 tcggccatgg catcaagccc tttcctgtga taacggtaaa ggcattcgcg gtacgaagag
     3241 tatgatttgt tcaacatgtt ctctaccagc caatagcggt tgaagtctga ctcaaatgct
     3301 ttccatccgt tttttgcccc cacggcattt tggctctcat tcacaatttg tcgggctttt
     3361 tcaaaatact ctgtgccacc gttgagcgag aaagtatcgt aatcgaagcc cagaattaca
     3421 taggcataat aggccaaaac actggtcagg ttatctttat tgtcggtttc agcatattca
     3481 atggtttgat attcttcata cttaaacctg aaatattcat cctgaacatt aagaatggtt
     3541 gttttgtacg atgagttgta aaccggcctt ttgctttgaa tatttagggt tccttcatat
     3601 tcgttcgaag aaagttggcg ggtaatctgg atggttatgc tacattcaat cctttcggca
     3661 tttgtataat tgtgcttggt ccatttttta ttgttcataa attcgtacag gtcggcctgc
     3721 atagtgcgga acacgttttt gttggcactg gtgagcttgt tcgaattgac gctgatgttg
     3781 caccgtaatt cctgagcctg tacaattgtg gttgtgaata ataaaaaaaa caggattgaa
     3841 aatagctgat ttatctttgt catttcattc taagttgtta ataatcaaat aaccgtaatt
     3901 tgatgttgaa aaaatgttat tgtgttttgc atgaccgctt ccctttttca aggcatcgtt
     3961 tttcggtgta aaaatagtaa tcgttgagga aattacaatc ccatttcttc aaccatccgg
     4021 tctacaatat ctacagcaac tttatctttt ctttttaact caaaattgta cgttttatta
     4081 tttctatcga tgatagtaat tttattggtg tcggcctgaa aacctgctcc tttatcgtta
     4141 agcgagttaa gtataatgaa atcgaggttt tttctgagca gttttgaggt tgcattgttt
     4201 acttcgtcgt tggtttcgag ggcgaatcct actagcaatt gattcttctt ttttattttt
     4261 cccagtgatg ccgcaatgtc aggattgggt ttcagggtta tggttaactc ttctgatgtg
     4321 cgttttattt ttgttgccga actaaccacc ggcgcaaaat cggccactgc tgcacacatt
     4381 acggctccgt cggccttcgg gaagatttgt acacattgct cgtacatctc ttgtgcagag
     4441 gtgatattta tgcgctgaat gtttttattt tttgtttgaa ccgagacggg gccggcaatc
     4501 aggtaaacat atgctccgcg tgaagccagc tcttcggcta tagcaaaacc cattttcccg
     4561 gaagagtaat ttccgatgaa gcggactggg tctattttct cgtgtgtagg gccggcggtg
     4621 actaaaaacc ttttgttatt cagttttttt tttcgctgaa gaaggaaatt atttcttcag
     4681 caatagcttc gggttcctgc attcgccctt ttccggtgag cccgctggca agttctccgg
     4741 tgctgggttc aatgattata ttgccgtatg tgcgaagcgt ttctaagttt ttttgagtag
     4801 aagggtgggc aaacatgtcg aggtccatgg ctggggcaac cattataggg cacttggctg
     4861 acaagtaagt ggtaattaac atgttgtcgg caatcccgtt agccattttc ccaatggttg
     4921 aggcggttgc cggagcaatg accataaggt cggcccataa tcccaaatcg acatggctgt
     4981 tccaagtgcc atcgttagcc ccaaaaaact cagagattac cggtttcccc gatagggcag
     5041 agagggtgac aggagtaata aattctttgc ccgatggagt tattaccact tgtacctcgg
     5101 ccccttcttt aatgaggcct cttaaaagtg tagctgcctt gtaagcagct atacttcctg
     5161 taattcctaa aataatatgt ttttctttca atcgcacgga gtgcaagttg cttagaatct
     5221 tactttacgg ctatctttca tcgggttacg atgataaatg cctttttcga tgaattcctg
     5281 agtcgcaatt aatgtcgact taggcaacct ttcgtaaaat ttcgaaattt cgatttgctc
     5341 acggttttca aatacttctt cgagattgtc ggtatacgaa gcaaattctg ataatttctg
     5401 gttcaattct tcttttaatt ccgaagcaat ctggttcgaa cgctttgcaa gaatcattac
     5461 tgattcgtag atattaccag tttcttcagc cagttgatcg atgttgcgag aaattgtaga
     5521 attcgcagct tttgttttct tataatccat attccttgtt gattgaacgt attctattta
     5581 tttatatttt ttagtattca gactgttggt gcttactggt ttttgcctac catgcttgtt
     5641 tcatcttctt ttaaacccaa aagttgctga atatgtttat aatcacggtt aacttctttt
     5701 aaatacttac tttgcgggaa ttcatcggca aaggtaaaat attcatcgcg ggcgtcaata
     5761 gttcgctctc tttttttctc ttctacgctg ttcactgcca ataaatactt cgatttgaat
     5821 agtagaaact tcaactcctc gcggtatttg gtcgatggat aatcgttgag gctgttgttt
     5881 aacgcaataa cagccgattt gtaatactcc atatcgtagt ataacttggc actgaggtac
     5941 gatttgtaaa caattttttc gtttagttca tcaattcgca tattggcttc ttcaaccttg
     6001 ttgctgtatg gataaaggtt tatgaagagc tgaaaagcat cgattgcttt ttgtgagatt
     6061 tcctgatcga ggcgaggatt gggtgaatct ttatagaaac acataccaac catgaat
//
LOCUS       DAOPUW010000021         6039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_13022_length_6039_cov_3.718416, whole genome shotgun sequence.
ACCESSION   DAOPUW010000021 DAOPUW010000000
VERSION     DAOPUW010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13022_length_6039_cov_3.718416"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="PKJ43_00770"
     CDS             <1..829
                     /locus_tag="PKJ43_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ71117.1"
                     /translation="TKKLYSANEITKSNVIDNYENIMALLETQQANPETKEKADQAIP
                     LVEQIVESCGVLDCNALVELYQPKFNENPNDVVFLKKVLKTMKKENCTDTELFGQISE
                     KLYSLEPSADAAFNMANAYVKKSNYEKAFEYYEKAYSVNETEPATRATYYYYAAMLGI
                     QHDRMQFARDMAREALKLKADYCEALMLIGEIYGQASKSFSSDEFEKQTVFWIAADYF
                     EKASRIANCANDGRSKANFYSGYFPNKEEVFFRSLTEGQRYTIGGWINETTTVRVKK"
     gene            816..1394
                     /gene="lptC"
                     /locus_tag="PKJ43_00775"
     CDS             816..1394
                     /gene="lptC"
                     /locus_tag="PKJ43_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNZ71118.1"
                     /translation="MSKSKNTSIQIFKSIAATLWVAVLFLSCQKNNLEQVKAFSHPPG
                     APEIVADSLHVFYSDSAVTRFSLECAKLLIYQDEEEPYNEFPEGFRLVQYDRNKKITS
                     SIEARYGKYFQKKELWEAKQNVIAVSEKGDTLKTELLYWDEKKEMIYTDQYVKIIQKE
                     QIITGIGLESDMQMNRYKIIQPKGTITVEVEE"
     gene            1401..2672
                     /locus_tag="PKJ43_00780"
     CDS             1401..2672
                     /locus_tag="PKJ43_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNZ71119.1"
                     /translation="MILLAPILISLLFLIFFTGMKNAYINVNKLSIEIEKHKSSFKSN
                     LLTIFTSEPQYFIATMQIGYIITLVCFELLMAWQILPILENNIPFIFVNLLIFILITS
                     PILLIANGIISETLFKTYSAKMLSFFALPVLCFYLIFYPIAYVYIHLSKWIIKNIFRQ
                     EVISENEKQLYTMSDLGHLINHPTSKNGTIEQEEEDTEVELFKNALDFSKVKVRDCMI
                     PRTDIEALELTSGIEELREKFIDTGFSKILIYNDNADNIIGYVHSSQMYKTPKKIKEV
                     LHGVSYVPETMMASTLLSTFTKQHKSIAVVVDEFGGTSGIVTTEDVLEEIFGEIEDEH
                     DTDDLIIKNIKDNEWILSARHEISLLNSKYHFNLPENDEYETIAGLIITQNEEIPKPK
                     SIIEIENFEFKVLKASETRIELVSMKIVTNN"
     gene            2773..4887
                     /locus_tag="PKJ43_00785"
     CDS             2773..4887
                     /locus_tag="PKJ43_00785"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNZ71120.1"
                     /translation="MATLQNLRNKAGVLLAAVIFIALAAFILGDFLQSGNSLMRNKQM
                     KIAEIDGESIDYIEFQSRFDEIANIYKANNQTNSLDEEAYQQILNQTWDATLQDKIMG
                     EVYDELGIDVTPTEMFDMVQGNNLHPIIQQIFGDPQTGRVNKANVIQFLKYIQDNPDS
                     PQKESWLNVEKQILTTKKLSKYSDLVSKALTANTLQAKQSLAEKDVVASLKFIQKKYA
                     AISDSSISVSSVEMKDYYKKHISDYEQKNQKVLAYVTFNIEPSEEDDQEALKYVSELK
                     SDFISAENNEQFVNANADSRFEQKYFTESELAPALSVWAFNSEIGSVYGPVKEIDTYK
                     LYKLNEVKMLPDSVKASHILIRVQTQAELQAANAKIDSLKKVIESGRQSFELAARMNS
                     QDGSASNGGDLGWFKRGMMVAQFENAAFNAEKDELVSVMTQFGVHLIKVTAQSSKTKN
                     VQLAVLDRKVTASTTTYQNIYAKASQFSAKAQDLDGFNRVVAEQKLSKRSIAVGENDR
                     SIPTLGPIRNMIRAAFLSGETGDLVVGQDRSPIFEADNKFVVAAIEGESEAGTKSFES
                     VKAAIHMAVAKEKKQNMLVDQFNKSRGSSIEQTASSLNLEVASASGFRPLYGSVNAIG
                     YEPAINGAVAKLGVAQQSKPIAGRNGVYIVELTDKTGPTAGDVKAEKKSLFQNSSYRA
                     SYQAYETLKKNAEIEDLRWKFY"
     gene            5002..5748
                     /locus_tag="PKJ43_00790"
     CDS             5002..5748
                     /locus_tag="PKJ43_00790"
                     /inference="COORDINATES: protein motif:HMM:NF036640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NigD-like protein"
                     /protein_id="HNZ71121.1"
                     /translation="METKDNTRKTIGMKTSTPTCVRRNKKMLFALLLSASLALTLFSC
                     EKDEDYYSLGDVWISMGFIDQANEGSFSIRLDNGDTLVPVSNAVPYFKTENNQRIMVN
                     YTILDEVNQSTKKFYVKVNNLYEILYKDIIELTALNNDSIGNDPVHIVDIWKSGSILN
                     VEFSFLGGGITHYINLAKPVENPNGQSQPIELELRHNARNDPFQYQLRSIVSFDLKSI
                     EIVGQDSIQFKIISTDYGGNKHTFSSTYTY"
     gene            complement(5780..>6039)
                     /locus_tag="PKJ43_00795"
     CDS             complement(5780..>6039)
                     /locus_tag="PKJ43_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNZ71122.1"
                     /translation="HETGYLLDPHGACGYRSLEEYLSDEEVGIFLETAHPAKFTETVE
                     EIVGKGNVPLPEKLAAFMKGKKQSINLDNDFEDFKAFLLNE"
BASE COUNT         2115 a         1205 c         1128 g         1591 t
ORIGIN      
        1 gacgaaaaaa ctctattcag ccaatgagat tacaaaatcg aatgtgatcg ataattatga
       61 aaacataatg gccctactcg aaacacagca ggccaaccct gaaaccaaag agaaggcaga
      121 tcaggcaata cctttggttg agcaaatcgt agaaagttgc ggcgttctcg actgtaatgc
      181 tttggtagaa ttatatcagc ccaaattcaa tgaaaacccg aacgatgttg tgttcttgaa
      241 aaaagtgctg aaaaccatga aaaaggaaaa ttgtaccgat actgaactgt tcggtcaaat
      301 ttctgaaaaa ttatacagtt tggaaccttc tgccgacgct gctttcaaca tggctaatgc
      361 ctacgtaaag aaaagcaatt acgaaaaagc tttcgaatat tacgaaaaag catactcggt
      421 taacgaaaca gaacctgcaa cccgtgcaac atattactac tatgccgcaa tgcttggaat
      481 acagcacgac cgcatgcagt ttgcccgcga catggctcgc gaagcactaa aattgaaagc
      541 cgattattgc gaagccctta tgcttattgg cgaaatatat ggtcaagcca gtaaaagttt
      601 cagttccgat gagtttgaaa aacaaactgt attctggatt gctgccgact attttgaaaa
      661 agcttcgaga attgcaaact gtgccaacga cgggagaagc aaagctaact tctactcggg
      721 ctacttccca aataaagaag aagtattctt tagaagctta actgaaggcc agcgctacac
      781 cattggtgga tggattaacg aaacaacaac cgttcgtgtc aaaaagtaaa aatacatcta
      841 tacaaatatt taaaagcatt gcagccactc tgtgggttgc agtgcttttt ttgtcgtgtc
      901 aaaaaaacaa cctcgaacaa gttaaggcat tttcgcaccc cccaggtgca ccagaaatag
      961 ttgccgacag cctgcatgtt ttttattcag actctgcagt aacccgcttc agcctcgagt
     1021 gtgccaaact cctcatatat caagacgaag aagaaccata caatgaattt cctgaagggt
     1081 tcagactagt tcagtacgac aggaataaaa agataacctc gagcattgaa gcccgatatg
     1141 gcaaatattt ccagaaaaaa gaactttggg aggcgaaaca aaacgttatt gccgtctctg
     1201 aaaagggtga tacattaaaa acagaattgc tatattggga tgagaaaaaa gagatgattt
     1261 atactgacca atatgtgaaa atcattcaaa aagaacaaat tattaccgga attgggttgg
     1321 aatcggatat gcaaatgaac cgatataaaa ttatacaacc taaagggacg ataactgttg
     1381 aagttgaaga ataaaattga atgatactgc ttgctcccat attaatttca cttctttttc
     1441 tcattttctt taccgggatg aagaatgctt atatcaatgt gaacaagcta agcatagaaa
     1501 ttgagaagca taaaagctca ttcaaatcaa atttacttac catatttacc tccgaacctc
     1561 aatactttat cgccaccatg caaattggat atataatcac gctggtttgc ttcgaattat
     1621 taatggcatg gcaaatactc ccaattctag aaaacaatat tccatttatt ttcgttaacc
     1681 tgttaatttt cattcttata acaagcccta tattattgat agcaaacggt attatctccg
     1741 agactctctt caaaacctac tctgcaaaaa tgttatcatt ctttgcgttg cccgtgttgt
     1801 gtttctattt aatattttac ccaatagctt acgtctatat acacctttcc aaatggataa
     1861 ttaaaaacat attccgacag gaagtaatat cggaaaacga aaagcaactt tacacgatga
     1921 gtgatttggg acatttgatt aatcatccga catcaaaaaa cggaacaatt gaacaggaag
     1981 aagaagacac tgaagttgaa ctatttaaaa atgccctcga tttttcaaaa gtaaaagtgc
     2041 gcgattgcat gattccccga accgacattg aagcactgga attaacatcg ggcatagaag
     2101 aactaagaga gaagttcatc gacacggggt tctccaaaat acttatttac aacgacaatg
     2161 ctgacaatat aattgggtac gtgcattcat cacagatgta caaaaccccc aaaaaaataa
     2221 aagaagtact ccacggagtt tcatatgtcc ctgaaacaat gatggccagt acactcttaa
     2281 gcactttcac caaacaacat aaatcgattg ccgttgttgt tgatgaattt ggcggaactt
     2341 caggtattgt gaccaccgaa gatgtgcttg aagaaatatt cggagagata gaagacgaac
     2401 acgacacaga cgacttgata atcaaaaaca taaaagataa tgaatggata ctttcagccc
     2461 ggcatgagat aagtctccta aactcaaaat accatttcaa tttgcctgaa aacgatgaat
     2521 atgaaaccat tgccggatta ataataaccc agaatgaaga aatacccaaa ccaaaatcaa
     2581 ttattgagat agaaaacttt gaatttaagg ttttgaaagc gtccgaaaca cgaatagaac
     2641 tggtatcgat gaaaattgtt acgaacaatt aaaaatcaag aataactctg attaggatag
     2701 aatagtaaat ttactatctt cgtagcctaa aaattttaac caacaattac attctaaata
     2761 agaaatattc aaatggcaac tttacaaaat ttgagaaaca aagccggtgt actactagcg
     2821 gccgtaattt tcattgcgtt ggcagccttt atccttggcg atttcctcca gtcgggcaac
     2881 tcactgatga gaaacaaaca gatgaaaatt gctgaaatcg acggcgaatc gattgactat
     2941 atcgaatttc aatcgcgctt cgatgagatt gctaatattt ataaagcaaa caaccaaact
     3001 aactcgttag acgaggaagc ttatcagcaa attttaaacc agacctggga cgccacgttg
     3061 caagacaaaa tcatgggcga agtatatgat gaattgggta tagatgttac cccaaccgaa
     3121 atgttcgaca tggttcaggg aaacaacctt cacccaataa tccagcaaat atttggcgac
     3181 ccacaaactg gcagggttaa caaagccaac gtaattcaat tcctgaaata cattcaggat
     3241 aacccggatt ctccacaaaa ggaatcgtgg ttgaacgttg aaaagcagat tctaacaacc
     3301 aagaaactct cgaaatattc agatctcgta tccaaagcac ttactgctaa tacattacaa
     3361 gcaaaacaaa gtttagctga aaaagatgtt gttgcgagtc tgaaatttat tcagaaaaaa
     3421 tatgctgcaa tttcagatag ttctatttcg gtttcttcag tagaaatgaa agattactac
     3481 aaaaaacata tttctgatta cgaacagaaa aatcagaaag tattggctta tgtaactttc
     3541 aatattgaac cttcagaaga agacgatcaa gaagccctga aatatgttag tgaattgaaa
     3601 tcggatttta tctcagccga aaacaacgaa caatttgtta atgccaacgc tgatagccgt
     3661 ttcgaacaaa aatattttac tgaatcagaa cttgccccag cactttcagt atgggcattc
     3721 aattctgaaa ttggctctgt atatggccct gttaaagaaa tcgacactta taaattatac
     3781 aaactgaacg aagtaaaaat gttgcccgac tctgtaaaag ccagccacat tttaatcaga
     3841 gttcaaactc aagccgaatt acaggcagct aatgctaaaa tcgacagttt gaaaaaagtt
     3901 atcgaatcgg gaagacagag ttttgaatta gcagcccgca tgaattcaca agacggcagc
     3961 gcatcgaacg gtggagacct tggttggttt aaacgcggca tgatggtagc tcaattcgaa
     4021 aacgctgcat tcaatgctga aaaagacgaa ttggtgagtg ttatgaccca atttggtgta
     4081 cacctcatta aagttactgc acaaagttca aaaacgaaaa acgttcagtt agccgttctc
     4141 gacagaaaag taacagcaag cacaaccact tatcaaaata tctacgcaaa agcaagtcaa
     4201 ttttcggcta aagctcagga tcttgatgga ttcaaccgtg tagttgcaga acaaaaactt
     4261 agcaaacgct ctattgctgt aggtgaaaac gatcgttcga ttcctacttt aggccccatc
     4321 cgcaacatga tccgcgcagc attcctcagc ggagaaactg gtgaccttgt tgttggacaa
     4381 gaccgctctc caattttcga agcagacaat aaatttgttg ttgctgcaat tgaaggagaa
     4441 tcagaagctg gaacaaaatc atttgaatcg gtaaaagctg ctattcacat ggctgttgca
     4501 aaagagaaga aacaaaatat gttggtcgat cagttcaata aatcgcgtgg ttctagcatt
     4561 gaacaaactg catcttcact taatttggaa gtagccagcg ctagcggttt ccgaccatta
     4621 tacggatcgg taaatgctat tggctacgaa cctgctatta acggagcagt tgctaaatta
     4681 ggcgttgcac aacaatctaa acctattgcc ggacgtaatg gcgtttatat tgttgaacta
     4741 accgataaaa ccgggcctac tgctggcgac gttaaagccg aaaagaaaag cttgttccaa
     4801 aattctagtt acagagctag ttaccaagct tacgaaacac ttaaaaagaa tgctgaaata
     4861 gaagatctcc gttggaaatt ctattaatag aaaaatattc aaagaaaagc tgttcaccct
     4921 gaacagcttt ttttttgcac agtacgcaac cattttgaat gcaaacgaat caattatctg
     4981 taaacaatta tttgattcac tatggaaaca aaagacaata cgagaaaaac aattgggatg
     5041 aaaacttcga caccaacgtg tgtgcgaaga aacaagaaga tgctgtttgc cttgctcttg
     5101 tcggcttcac tggcactaac cttattttca tgcgaaaaag atgaagacta ttactcgctt
     5161 ggcgatgtgt ggatatcgat gggctttatt gaccaggcaa acgaaggctc tttttcaata
     5221 aggctcgaca acggcgacac gctggtgccg gtttccaacg ccgttcccta tttcaaaaca
     5281 gagaataacc agcgcattat ggtgaattac accattcttg acgaggtaaa ccaatcgaca
     5341 aaaaagttct acgtaaaagt gaacaacctt tacgaaattc tgtacaaaga cattattgaa
     5401 ttaaccgctc tgaacaatga cagcattgga aatgaccccg ttcacattgt cgatatttgg
     5461 aaaagtggtt caattcttaa tgttgaattc agtttcttgg gcggaggaat tacccactac
     5521 atcaatcttg caaaaccagt ggaaaaccct aatggacaat ctcaacctat tgaactcgaa
     5581 ttgcgccaca atgcacgaaa tgatcctttc caataccagt tgcgaagcat tgtttcgttc
     5641 gaccttaaga gcattgaaat tgtagggcaa gactctattc aatttaaaat catttcaacc
     5701 gattacggcg ggaataagca tacgtttagc agcacctata cctattaaaa caaaagcggc
     5761 taaagccgct tttgttttgt tattcattaa gcaaaaatgc tttaaaatct tcaaagtcgt
     5821 tgtctaaatt aatgctctgc ttcttacctt tcataaaggc agcaagtttt tcgggcagag
     5881 gcacattgcc tttcccaaca atttcttcaa cagtttcggt aaatttggcg ggatgcgcag
     5941 tctcaaggaa aataccgact tcttcatctg acaaatactc ttcaaggcta cggtagccac
     6001 aagctccgtg cggatcgagc aaataacccg tttcatggt
//
LOCUS       DAOPUW010000022         6011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_13123_length_6011_cov_3.618200, whole genome shotgun sequence.
ACCESSION   DAOPUW010000022 DAOPUW010000000
VERSION     DAOPUW010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13123_length_6011_cov_3.618200"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(288..1538)
                     /locus_tag="PKJ43_00800"
     CDS             complement(288..1538)
                     /locus_tag="PKJ43_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HNZ71123.1"
                     /translation="MFALVDGNNFYVSCERVFNPKLEGRAVVVLSNNDGCVISRSNEA
                     KALGIKMAEPYFQHKDLYASNDVTAFSSNYTLYGDMSNRMMTILRNFTPDLEIYSIDE
                     AFLNLEGINANMHDYGKIIRATILKNIGIPVGVGIGPTKTLAKVANKIAKKTEGVFVI
                     DSEEVRVSSLKQTLIEDVWGIGRQYARKLQSMNIVTAYDFSRLDPNWVLHNMTVVGHR
                     TWEELNGKPCIPIEAMFQPKKNIATTRSFGKRLNDIDRIAQAVATHSVRCAEKLRKQH
                     SVANFVTVFIHNDPFSKTEKYFYQSITITLPVASNSNNDIVNAAICGLKKIFKPTIFY
                     KKSGVIVSGISSDDFVQGNLFETVDNSRQKKISQLSDRINHRYGQDTLKLAVQGGNND
                     WKLRHDKLSPCYTTRWNELLKVNS"
     gene            complement(1541..1987)
                     /gene="umuD"
                     /locus_tag="PKJ43_00805"
     CDS             complement(1541..1987)
                     /gene="umuD"
                     /locus_tag="PKJ43_00805"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HNZ71124.1"
                     /translation="MERKNNKISLEILDIPVKSEPFNPQLEGFVTAGFPSPANDYIEN
                     ELDLKMLLVRNESSTFFVQVRGDSMKDAGIYNDDILVVDKSLDPSDNSILVCFLDGEF
                     TVKRVSKIDGEFYLMPANSDFKPIKINKSSDFRLWGVVTYSIHKHR"
     gene            complement(2110..2703)
                     /gene="yihA"
                     /locus_tag="PKJ43_00810"
     CDS             complement(2110..2703)
                     /gene="yihA"
                     /locus_tag="PKJ43_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNZ71125.1"
                     /translation="MIIKSAEFIISSTEVEKCPKNNFPEYAFIGRSNVGKSSLINMLT
                     GFNSLAKVSGRPGKTRLINHFLINKEWYLVDLPGYGFAQVAKSEREKWEKFIRKYLAQ
                     RENLFCVFVLVDSRHEPQKNDLSFMEWLGENSIPFVIVFTKTDKLGKQAMKEFQDNYA
                     QKMLESWDELPDMFASSADTGAGRDEILDFIERVNKS"
     gene            complement(2759..3754)
                     /locus_tag="PKJ43_00815"
     CDS             complement(2759..3754)
                     /locus_tag="PKJ43_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HNZ71126.1"
                     /translation="MELYPLKFIPIYKERIWGGNKLHSLLGKDLDGKTNIGESWELSA
                     VEDSISVVSNGFLEGNDLQELIEIYMGDLVGEKVFEKFGLEFPLLIKLIDANEDLSIQ
                     VHPNDEIARERHSAFGKTEMWVALDGSAAPRLISGFNRDTNKNEFLEKLENNTLVDLF
                     NVETVKTGDVFFVPAGRVHAICAGNLVAEIQQTSDVTYRIYDYDRRDSQGNSRQLHLD
                     LSLDVIDYTKVKDAKINYESKENEPVELVSCNYFTTNRLLVNKPLGRDYYSIDSFVIY
                     LCTEGSCQIGEPGGIKETMKKGDTVLIPAALNSVEIIPFSEKAELLEIYIESELD"
     gene            complement(3775..4077)
                     /locus_tag="PKJ43_00820"
     CDS             complement(3775..4077)
                     /locus_tag="PKJ43_00820"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HNZ71127.1"
                     /translation="MINHVVLFKVKDFASDEEKNAVLSQIKAQLLALKDVIAELKYIE
                     VGTHYLNPSPSFDLCLISHFDNADDLQKYQVHPEHLKVGEIIKQVATARAAVDFEF"
     gene            4178..4822
                     /gene="udk"
                     /locus_tag="PKJ43_00825"
     CDS             4178..4822
                     /gene="udk"
                     /locus_tag="PKJ43_00825"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNZ71128.1"
                     /translation="MLIIGIAGGTGSGKTTVVNEIVKRLPKDDVVIIPQDAYYRDNKD
                     IPLEERKKMNYDHPDSIEFELLVEHLKILKTGQSIQQPIYSYLTCSRHQETIKVEPKH
                     VVIVEGILCLTQPELRKLMDIKVFVDCEADLRLSRVIQRDIIERGRGVDQTLDRYEKT
                     VRPMHLQFIEPSKHYADIIVPQGGQNIVAINILTQFIEKQLAEKKEYYARTNKH"
     gene            4800..5522
                     /locus_tag="PKJ43_00830"
     CDS             4800..5522
                     /locus_tag="PKJ43_00830"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNZ71129.1"
                     /translation="MLEQINIESVLFIDIETVPQFEGYDSLDEKFQKLWDKKSSHFRS
                     ENENPEDVYQRAGIYAEFGKIICISVGAIYQKQGEYRCRVKSYANHDEKKLLYEFVNM
                     LEGFSRREPVKLCAHNGQEFDYPYIARRCLINSVPVPKILDIAGAKPWDIKDTLLDTM
                     QLWKFGDYKNYTSLELLCAVFNIPTPKDDIDGSQVAQVYYIENDLDRIVKYCEKDVFA
                     LAQLFMRYQGKPILSPDAFESA"
     gene            5566..>6011
                     /locus_tag="PKJ43_00835"
     CDS             5566..>6011
                     /locus_tag="PKJ43_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71130.1"
                     /translation="MKENDENSTCCPPFNPDPWDDKMFEWNEKLFIRKKVKTFFFMPL
                     NFGSVMSKVIPILEAHGNCTKETMCLSDHTSRWNMDILLSVNSEIPGLENVKLNGKFY
                     SRVYEGNFNKTGEWCKHFENEASKKGFAVKKWYMWYTTCPKCAKKYG"
BASE COUNT         1858 a         1208 c         1103 g         1842 t
ORIGIN      
        1 aagataggaa gtcgctgttc cggtaaaaac tttaagcggt gctttgcgtg gcatgcgtct
       61 agtagtttat tgattccaac tgcggcaaaa gtataaaaat atcgaaagcg ttacttataa
      121 aagttatgat ttcttattaa cgttttttgc atcaccagaa gtttctcgat gcacaataac
      181 attcaagcgg ctgaaattca atcgattatt ttcagcaaaa atgaaatgct gatacttaca
      241 cttaatgtat tacatattga catctatctt gccgattttt ccctcactca actgtttacc
      301 tttaataatt cattccaacg ggttgtatag caaggcgata atttatcatg acgaagtttc
      361 caatcgttgt tgcctccctg cactgccaac tttaaggtat cctgtccata gcggtggttt
      421 attcggtcgc ttaattgcga tattttcttt tgacgtgaat tatcgactgt ttcaaacaaa
      481 tttccttgta caaaatcatc ggacgaaata ccacttacaa ttactcccga ttttttatag
      541 aaaatagttg gcttaaatat ctttttcagt ccgcatattg ctgcattaac tatatcatta
      601 ttagaattag aagcaacagg aagtgtaatc gtgatgcttt ggtaaaaata tttttcagtt
      661 ttcgaaaacg ggtcgttgtg tataaaaaca gttacaaaat tagccactga gtgttgtttc
      721 cgcaattttt ctgcacatcg aacagaatgt gttgcaacgg cttgggcgat tctatctata
      781 tcgttgagtc gctttccaaa actacgcgtt gtggcaatat ttttcttcgg ttgaaacata
      841 gcttcaatgg gaatacatgg ttttccgttt aactcttccc atgtacgatg cccaactact
      901 gtcatgttgt gtaaaaccca gtttggatcg agccgggaaa aatcgtaggc tgttactata
      961 ttcatcgatt gaagtttgcg cgcatactgg cggccaattc cccaaacatc ttcgattaaa
     1021 gtttgcttta gcgacgacac ccgcacttct tctgaatcaa ttacaaagac accttcagtt
     1081 tttttcgcaa ttttgttggc cacttttgca agtgttttag ttggaccaat gccaacaccc
     1141 acgggtatgc ctatgttttt taggatggtt gcacgaataa ttttcccata gtcgtgcatg
     1201 ttggcgttta tgccctccaa attcaggaac gcttcatcaa ttgaatatat ttctaaatca
     1261 ggggtaaaat tcctcaatat ggtcatcatt cggttcgaca tatcaccata aagagtgtaa
     1321 tttgatgaaa acgcagtgac atcattcgaa gcatacaaat ccttatgttg aaagtaaggc
     1381 tctgccatct taattcccaa cgctttagcc tcgtttgaac gtgaaattac acatccgtcg
     1441 ttgttcgaca aaacaacaac tgcccgccct tctagtttcg gattaaaaac cctttcgcac
     1501 gatacgtaaa agttattccc gtcgaccagc gcaaacatct ctacctgtgt ttatgaatgg
     1561 aatacgtcac aacgccccaa agcctgaaat ctgatgattt attgattttt attggcttaa
     1621 aatctgaatt ggcaggcatg agataaaatt caccatcaat cttacttacc cgctttaccg
     1681 taaactctcc gtcgaggaaa cacactaata tggaattatc agacggatcg agcgatttat
     1741 caacaacaag aatatcatca ttataaatcc ctgcatcctt cattgagtcg cccctgactt
     1801 gtacaaaaaa agtagaactt tcgttccgga ccaataacat tttcagatcg agctcatttt
     1861 caatatagtc gttagctggc gatggaaaac cggctgttac aaaaccttcg agttgcggat
     1921 tgaacggttc actttttact ggtatgtcga gaatttccag cgagatttta ttattttttc
     1981 gttccatcgt gcttggattt ttacaaaggt aatgctttag catttactgt gagcaataaa
     2041 tgatacgtaa caaatgcttg aatttattac tcatttaaga catttattat ttgcccacaa
     2101 taatggtttt caagatttat tcactcgctc aatgaaatcg aggatttcgt cacgccctgc
     2161 tccggtatcg gccgacgatg caaacatatc aggcaattca tcccagcttt caagcatttt
     2221 ttgggcataa ttatcctgaa attctttcat cgcctgtttg cccagtttgt cggttttggt
     2281 aaatacaatt acaaaaggaa tagaattttc gcccaaccat tccataaacg aaagatcgtt
     2341 tttttgaggc tcgtgacgcg aatcgacaag cacaaaaaca caaaacaggt tttcgcgctg
     2401 agcaaggtat tttctgatga atttttccca cttttctcgc tcagacttgg ctacttgagc
     2461 gaaaccatac cctggaagat cgactaaata ccattcttta ttaatcagaa aatggtttat
     2521 caaccttgtt ttccctggcc ttcccgaaac ttttgctaaa ctattaaaac cggtaagcat
     2581 atttataagc gatgacttgc ctacattaga ccgcccgata aacgcatatt ctggaaaatt
     2641 gtttttgggg catttttcaa cttcggtact gcttataatg aattcggctg actttataat
     2701 catgcaaatg ggttttaagg atttaaacta tgggtaatta atcaattata caatttaatc
     2761 aatctaattc agactctata tatatttcga gtaattctgc tttttcggaa aatggaataa
     2821 tttctaccga gtttagggct gcaggaataa gcacggtatc cccttttttc atggtttctt
     2881 taattccacc aggttctcca atctggcaac ttccttcggt acataaatag ataacaaaac
     2941 tgtcgataga ataataatca cgtcccaacg gtttattgac taaaagccta ttggttgtaa
     3001 agtagttaca agagaccaac tcaactggct cattttcctt actttcataa ttaattttcg
     3061 catctttcac ctttgtataa tcaattacat cgagcgaaag atcgagatga agttggcggc
     3121 tgttcccctg gctgtcacgg cgatcataat cgtagatacg ataggttaca tcggacgttt
     3181 gctggatttc ggcaacaagg tttcctgcgc aaattgcatg gactcggcct gctggcacaa
     3241 aaaacacatc tcccgttttc acagtttcga cattaaataa gtcgacaagt gtgttatttt
     3301 caagcttttc aagaaattca tttttattgg tatcccgatt gaaacctgaa ataaggcgag
     3361 gggctgcgct gccatccaaa gctacccaca tttcagtttt gccaaaagcg ctgtgacgtt
     3421 cacgtgcaat ttcatcgttt gggtgaacct gaatcgataa atcttcgttg gcatcgataa
     3481 gttttataag caacggaaat tcaaggccaa acttttcaaa gactttttca cccaccaaat
     3541 cacccatata aatctcaata agttcctgca aatcattccc ttcgagaaaa ccattggaaa
     3601 caaccgaaat actgtcttca acagccgaaa gctcccagct ttcaccaata tttgttttac
     3661 catcaagatc ttttccaaga agggaatgta acttattccc gccccagatg cgctctttat
     3721 atatagggat aaactttaac ggatataatt ccatgatatt cagacttcta ttttttaaaa
     3781 ttcaaaatcg acagccgcac gtgcagttgc aacttgtttt attatctctc caaccttaag
     3841 atgctcagga tgaacctggt acttttgaag atcgtcagca ttatcgaaat gagaaatgag
     3901 gcacaaatcg aaagaaggcg aagggttcag atagtgagta cccacttcaa tgtattttag
     3961 ctcggcaata acgtccttca gagcaagcaa ttgtgcttta atttgagaaa gcactgcatt
     4021 tttttcttca tcagaagcga aatcttttac cttgaaaagg acaacatggt ttatcatttt
     4081 gctgaaattt attgttaatc tatctttttg aaaagtttgg caaatatact gctcatattt
     4141 ttgtgataat aatgtaattt tgaaaaaaag gttaaacatg ttaataatag ggattgccgg
     4201 aggaaccggt tcgggaaaaa caacagttgt aaatgaaata gttaagcgtc ttccaaaaga
     4261 tgacgttgta attatcccgc aagacgcata ttaccgagac aacaaagata ttccactgga
     4321 agagcggaaa aaaatgaatt acgaccaccc cgattcgata gaatttgagc tgttggttga
     4381 acacctgaaa atattgaaaa ccggacaatc tattcagcag ccaatttact catacctaac
     4441 ctgttctagg catcaggaaa ctattaaagt tgagccaaag cacgtggtta ttgtggaagg
     4501 tattttgtgt cttacccaac cagaattgag aaaactgatg gatatcaagg tcttcgttga
     4561 ttgtgaagcc gatttacgac tttcgagggt aatacagcgt gacataattg aacgaggccg
     4621 cggggttgac caaaccctcg acagatatga aaaaacggta cgcccaatgc acctgcaatt
     4681 tattgagcca agtaaacact atgccgacat cattgtacct cagggcggac aaaatattgt
     4741 tgccattaac atacttaccc aatttataga gaaacagtta gctgaaaaaa aagaatacta
     4801 tgctcgaaca aataaacatt gaatcggtat tgtttatcga cattgaaacc gtaccgcaat
     4861 ttgaaggtta tgacagcctc gatgaaaaat ttcaaaaact atgggataaa aaatcgtcgc
     4921 acttccgttc agaaaatgaa aatccggaag atgtgtatca acgagcagga atatatgccg
     4981 aatttggtaa aattatatgc atctcggtag gtgcaatata ccaaaaacaa ggcgaatacc
     5041 gctgccgggt taagtcgtat gcaaatcacg acgagaaaaa attactttat gaatttgtaa
     5101 atatgcttga aggattctcg cgccgcgaac ctgtaaaact atgcgctcac aacggccagg
     5161 agttcgacta tccttatatt gcccgacgtt gccttatcaa ttcggttcct gttcctaaaa
     5221 ttcttgatat tgcgggtgca aaaccatggg acattaaaga tactttacta gataccatgc
     5281 aactatggaa gtttggtgac tataaaaatt acacatcgct tgaattactc tgtgctgttt
     5341 tcaatattcc aaccccaaag gacgatattg atggcagcca ggttgcacaa gtctattaca
     5401 tagaaaatga tctcgaccgg attgtgaagt attgtgaaaa agatgttttt gcgttagctc
     5461 agctttttat gcgctatcaa ggcaagccga ttctttctcc agacgctttt gaaagtgcct
     5521 aaaatgcgta tttcaatcaa ataaatcaca attaaattca ctgctatgaa agaaaacgat
     5581 gaaaattcaa cttgttgtcc cccatttaac cctgatcctt gggatgataa aatgtttgaa
     5641 tggaacgaaa aattgttcat tcgtaaaaaa gtaaaaacgt tcttctttat gcctcttaat
     5701 tttggctcgg ttatgagcaa agtaatccca atacttgaag cacatggcaa ttgcacaaag
     5761 gaaacaatgt gcctttcgga tcatacatcg cgttggaata tggacatttt gctttctgtt
     5821 aatagcgaaa ttccaggctt ggaaaatgtg aaactcaatg gcaagtttta cagccgggtt
     5881 tacgaaggaa atttcaacaa aaccggagaa tggtgcaaac acttcgagaa cgaagcttcg
     5941 aagaaagggt ttgcagtaaa aaaatggtac atgtggtata caacttgccc caaatgtgct
     6001 aaaaaatatg g
//
LOCUS       DAOPUW010000023         5993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_13187_length_5993_cov_2.990906, whole genome shotgun sequence.
ACCESSION   DAOPUW010000023 DAOPUW010000000
VERSION     DAOPUW010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13187_length_5993_cov_2.990906"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..3200
                     /locus_tag="PKJ43_00840"
     CDS             42..3200
                     /locus_tag="PKJ43_00840"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNZ71131.1"
                     /translation="MFRRGKIIFALFVFFQIVNNFSVSAQVVLKSGIDYPAEIENPEL
                     LGINKEPYHATLMPYANLQEALVAKRHASSLCRSLNGQWKFNWVPTPDQRPVDFYKPD
                     FDVSDWKELPVPSNWEVHGYGTPFYRNLGYTIKKDYPNVMSEPEKWYTSYKERNPVGS
                     YRRDFEVPADWKGRRNFITFDGVDCAFFLWINGEKVGFSVNSRNAAEFDLTNYLKTGK
                     NTIAVEVYQYSSGTWLEDQDMFRLHGIFRNVTLWSAPQVHIRDFFVKTDLDKEYKDAT
                     LDITAKIKNYSDKTAKVQTLIASLYDKEGKEIARGIAFSSALKPNQEETLNVKILVRN
                     PLKWTAETPNLYTVVLTNSEGEILSSKIGFRKIEIVGRVFMVNGVPIKLKGVNRHEHW
                     SDVGHAITEEQMIHDLQVIKQGNCNHIRTSHYCDDPRWYELCDEWGIWLVAEANVECH
                     GYDGRFDNEPTIKSAIVDRNVANIENFKNHPSVIIWSLGNECGWGATPNFVAAMNAVK
                     AMDPSRPIHYERFGIGKNNPADLDGRMYGTAQDYARTAQDKSLTKPFYICEFVHAMFN
                     SMGSLDEYSEVFDNNPEILGGAIWEYQDQALWNKRDPNRPILAYGGGFGEAPNDHYFI
                     HKGVVSYDRTTEGNRTKPHFPEMKKAFQWIDMDLVDPAAGEVQVRNKYQFTSLDKFDA
                     WWSLTENGKEIDKGSLNVRNQWGRVIARVPYKIEQPKAGAEYFVRVSFTLKEKTLWAD
                     KGYEVASEQFKLPINTLPVVETKVTQPVKLVQNDQVATVSGSNFSVSFDKKSGFISQL
                     TQNGTNLLVADGAPKLHLWRAAHRTDDDWAFRQWEKFGVTTLQYTPVDFKVETVDNST
                     VKITSTTKADGKEGFGVYHTATYLVKGDGSIKVDKQVQFVGFKINLAKIGVRMMLDKK
                     LDRMTFFGRGPFENYSDRKSAAEVGLYEIGVNEQYEYEKPMERGNHEEVRWAKLSGKD
                     MPSLLIQSDERLMQVAALPHTDEQMNPVEYKIDLPASTATVLSVSTKTLGVGSNSCGP
                     QPLEQFKVAAENTQFSYTLILR"
     gene            3292..5769
                     /locus_tag="PKJ43_00845"
     CDS             3292..5769
                     /locus_tag="PKJ43_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNZ71132.1"
                     /translation="MKKIVISSMLCFLFVISGLAQTYKKNASGIEATVNKTTIEVSFF
                     SPSIVRVVKSPEGKSFKKESLSVVKTPQKTAFTINQQGDNLSLKSEKMVAVLNLKSGK
                     MVFSTLKGEPLLAEKESGSSFTDFNDAGTPTFSVSQSYVLDKEEAIYGLGILQNGKMS
                     QRNQEVYMVQNNTWDFVTFFQSVKGYGLFWDNYSPTTFKDTPSETSFRSEVGEGVDYY
                     FMYGGNADGVVAQMRDLTGQVPMFPLWTYGFWQSKERYKTQEEIVGVVKKHRELGVPL
                     DGIIQDWQYWGSNYLWNAMEFLNEEFPKPQQMVDDIHGMNAHLIISIWSSFGPQTKQF
                     REMDPKGMLLDMGTWPQSGLTSWPPRKEYPSGVKPYDPFNPEARSIYWRHLNEGLFKL
                     GIDGWWMDSTEPDHLDARPQDFDNKTYLGSLRKVRNAFPLAAVGGVYDNQRASTSDKR
                     VFILTRSAFAGQQRYGANTWSGDVNSSWENLRNQIPAGLNFSLCGIPHWNSDIGGFFA
                     GAYNRSWNDGSAAKNPLFQELYVRWLQFGAFNPMMRSHGTDLSREIYHFGQKGEPIYD
                     AIAQTIQMRYSLLPYIYSTAWEVTNKQSSFMRALVMDFPKDKKVWDMNDQFMFGNSIL
                     VAPIVQAQYTPEKIVKSNEETGWDRKEGDGKGNKDAVDFNQKKSSTTYLPAGVVWYDF
                     WTNEKFNGGQDVNKETTINTIPVYIKAGSILPIGPQVQYAEEKKWDNLEIRVYEGANG
                     EFTLYEDENDNYNYEKGMYSIVKFTWNDAKKELTIAPRQGSFPGMLAERNFNIVKVSV
                     KNGIGNDFETEIVKAVNYTGEKVTVKL"
     gene            5803..>5993
                     /locus_tag="PKJ43_00850"
     CDS             5803..>5993
                     /locus_tag="PKJ43_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71133.1"
                     /translation="MNNTKRIVNIAMALMFTIYINGIAQYPIIQTKFTADPAPMVYND
                     TVYLYTSHDEDDAQGFKML"
BASE COUNT         1848 a         1215 c         1358 g         1572 t
ORIGIN      
        1 ttttattcga ttagaattga tagaatctaa aaacctatat catgtttaga agaggaaaaa
       61 tcatattcgc actatttgtt ttctttcaaa tagtcaacaa ttttagtgtt tcggcacagg
      121 ttgttttaaa gtcagggatc gattatcctg ctgaaatcga aaatcccgaa ttactgggca
      181 taaataaaga accctaccat gccacgttga tgccgtatgc caatctgcag gaagctttgg
      241 tggcaaagcg acatgcttcg tcgttatgcc gtagcctgaa tggtcaatgg aaatttaatt
      301 gggtgccaac gccagatcaa aggccagtcg atttttataa accagacttc gatgtttcgg
      361 attggaaaga gctacctgta ccttctaact gggaagtgca tggttacgga acaccatttt
      421 accgcaatct gggctatacc attaaaaagg attatccaaa tgtaatgagc gaacctgaaa
      481 aatggtacac ttcgtacaag gagcgtaacc cggtgggtag ttaccgcaga gacttcgaag
      541 tgcctgccga ctggaaaggc cgccgcaatt ttattacctt cgacggtgtt gactgcgctt
      601 tctttctttg gataaacggc gaaaaagttg gttttagcgt aaacagccgt aatgcggcag
      661 aattcgactt gaccaactat ttgaaaacgg gaaaaaacac aatagcggtt gaagtttatc
      721 aatacagctc aggaacttgg ctcgaagatc aggacatgtt ccgcttgcat ggtattttca
      781 gaaatgtaac gctgtggagt gcgcctcagg tacacattcg cgactttttt gtgaaaaccg
      841 atcttgacaa agaatacaaa gatgcgacgc ttgatattac tgcaaaaatt aaaaactaca
      901 gcgacaaaac agctaaagta caaacattaa tagcatctct ttacgacaaa gagggcaaag
      961 aaattgccag aggaatcgca ttcagttcgg cacttaagcc caatcaagaa gagactttaa
     1021 atgtaaaaat cctagtacgc aatccgctga aatggactgc tgaaacccct aatttataca
     1081 ccgttgtttt gaccaattca gaaggtgaaa ttctatcgtc gaaaattggt ttccgaaaaa
     1141 tagaaattgt cggacgagtc tttatggtga atggagtacc gattaaattg aaaggggtga
     1201 accgccacga acattggtcg gatgtaggcc atgctattac tgaagaacag atgattcacg
     1261 accttcaggt gataaaacaa ggaaactgta accacatacg caccagccac tattgcgacg
     1321 atcctcgctg gtacgagctt tgcgacgaat ggggaatttg gctggtagcc gaagccaatg
     1381 tagaatgcca tggctacgac ggacgtttcg ataatgaacc aaccattaaa tcggcaattg
     1441 ttgaccgcaa tgtggccaac atcgaaaact tcaaaaacca cccttcggta attatctggt
     1501 cgctgggtaa cgagtgcgga tggggtgcta ctccaaattt tgtagctgca atgaatgcag
     1561 taaaagcaat ggatccttca aggccaatac actacgaacg tttcggaatt gggaaaaata
     1621 acccggccga ccttgatggc cgcatgtatg gaacagctca ggattatgcc cggacagctc
     1681 aggataagag tctcacaaag ccattctata tctgcgagtt tgttcatgct atgttcaact
     1741 cgatgggctc gctcgatgaa tattcagaag ttttcgataa caatcccgag attttgggtg
     1801 gtgctatttg ggaataccag gatcaggcac tttggaacaa acgcgaccct aaccggccaa
     1861 ttttggctta cggtggtggc tttggcgagg ctccaaacga tcactatttt attcacaaag
     1921 gggtggtttc gtacgaccgg acaactgaag ggaatcgcac taaaccgcat ttccctgaaa
     1981 tgaaaaaggc atttcaatgg attgatatgg atttagttga ccctgcagct ggtgaagttc
     2041 aggttcgaaa caaataccaa ttcacctcgt tggataagtt tgatgcttgg tggagtttaa
     2101 ccgaaaatgg gaaagaaatt gataaaggat cgttaaatgt tcgtaaccaa tggggacgtg
     2161 tcattgctcg ggttccttat aaaattgaac aacccaaagc aggtgctgaa tacttcgtgc
     2221 gagtttcgtt cactctaaaa gaaaaaacgc tttgggccga caaaggatat gaagtcgctt
     2281 ctgaacagtt taaactgcca atcaacaccc ttcctgttgt tgaaactaaa gttacacagc
     2341 ccgtaaaact ggttcaaaat gatcaggtag ctactgtttc tggtagcaat ttttctgtga
     2401 gtttcgataa gaagtctgga ttcattagcc aattaactca aaatggaacc aatcttttgg
     2461 tggccgatgg tgctccaaaa ttgcatttgt ggcgtgctgc acatcgcacc gacgatgact
     2521 gggctttccg tcaatgggaa aaatttggcg taacaacgct tcaatatact cctgtcgatt
     2581 ttaaagttga aactgtcgat aactcaacag tgaaaattac ttcgacaaca aaagccgatg
     2641 gaaaagaagg ttttggggtt tatcatacag caacttacct cgtaaaaggc gatggttcga
     2701 taaaggttga caaacaggta caatttgtag gtttcaaaat taacctggca aaaattgggg
     2761 tgcgtatgat gcttgacaaa aagctcgacc gcatgacttt ctttggccgt ggtcctttcg
     2821 agaactattc cgatcgtaaa agcgctgccg aagttgggct ttatgagata ggtgtaaacg
     2881 aacaatacga atacgaaaag ccaatggaac gaggcaatca cgaagaagtc cgttgggcaa
     2941 aattgagtgg taaagatatg ccttcactgt tgattcagtc tgatgaaagg ctgatgcaag
     3001 ttgctgcact gcctcatacc gatgaacaaa tgaacccggt tgagtataaa attgatttgc
     3061 ctgcaagcac ggcaaccgtt ttgagtgttt caacaaaaac gctgggtgtt ggttctaata
     3121 gttgtggccc tcaaccgctc gaacagttca aagttgctgc tgagaatact caatttagtt
     3181 ataccctcat ccttcgttga gagaggtgta ataaattagt tagtagtttg ttttcaacaa
     3241 aatttactta ataaaatgaa aaaaatgaaa aaaatgaaaa aaatgaaaaa aatgaaaaaa
     3301 atagttattt cgagtatgct ttgcttcctg tttgtaattt caggtttggc acaaacatat
     3361 aagaaaaacg catcaggcat tgaagcaacc gtcaataaaa caactattga agtttctttc
     3421 tttagtcctt ccattgtcag ggttgtgaaa tcgcccgaag ggaaaagctt taaaaaagaa
     3481 agcctttcag tggttaaaac gccacaaaaa actgcattta cgatcaatca acaaggagat
     3541 aacctttcat tgaaaagcga gaaaatggtc gctgtgctga atcttaaaag tggtaaaatg
     3601 gttttttcaa cgcttaaagg agaaccactt ttagctgaaa aagaatcggg tagttcattt
     3661 acagatttca acgatgccgg tacgcctact ttcagcgtca gccaatcgta tgtgcttgat
     3721 aaagaagagg ccatttacgg tttgggaatc ctccaaaatg gcaaaatgtc gcagcgcaat
     3781 caggaagttt acatggttca aaacaatacc tgggattttg ttaccttttt ccagtcagtc
     3841 aaaggctatg gtcttttctg ggacaactat tcgcccacaa catttaagga cacgccttcc
     3901 gaaacctcct tccgatcgga agttggcgaa ggagtcgact attatttcat gtacggtggc
     3961 aatgccgatg gggttgtggc tcaaatgcgc gaccttactg gacaggtgcc catgttcccc
     4021 ttgtggactt acggtttctg gcaaagtaaa gaacgttata aaactcagga agaaattgtt
     4081 ggtgttgtga aaaaacaccg tgaattggga gttcctctcg acggaattat tcaagattgg
     4141 caatactggg gcagcaatta cttgtggaat gccatggaat tcctaaacga agaatttccg
     4201 aagcctcaac aaatggttga tgacattcac ggcatgaatg cacatctaat catctccatt
     4261 tggtcgtcgt tcgggccaca aaccaagcaa tttcgcgaaa tggatcccaa aggaatgctt
     4321 ctcgacatgg gtacttggcc gcaatcgggt cttaccagct ggcctcctcg aaaagaatat
     4381 ccatcgggcg taaaacctta tgacccattc aatcccgaag ctcgaagcat ttattggagg
     4441 cacctgaacg agggtttgtt taagctgggt atcgacggat ggtggatgga ctcgaccgag
     4501 cccgatcact tggatgcaag gcctcaggat tttgacaaca aaacctattt gggatcgttg
     4561 cgcaaagtac gaaatgcttt cccattggca gctgttggcg gcgtttacga caaccaacga
     4621 gcttcaactt ccgataaacg ggtattcatt ttaacccgtt ctgcttttgc cgggcaacaa
     4681 cgttatggag ccaacacttg gtcgggtgat gtgaattcgt cttgggaaaa tttacggaat
     4741 caaattccgg ccggactcaa tttttctttg tgcggtattc ctcattggaa cagcgacatc
     4801 ggtggcttct ttgccggagc atacaaccgt tcgtggaacg atggttcagc tgcaaagaac
     4861 ccacttttcc aagaattgta cgtgcgttgg ttgcaatttg gtgcgttcaa ccccatgatg
     4921 cgttcgcacg gaaccgactt gtcgcgcgaa atttatcact tcggtcaaaa aggagaaccg
     4981 atttacgacg ccatcgccca aaccattcaa atgcgttact ctttattgcc ttacatttat
     5041 tctacagcgt gggaagtaac gaacaagcaa tcgtcgttta tgcgtgcctt ggttatggat
     5101 ttccccaaag acaagaaggt ttgggatatg aacgatcagt ttatgttcgg aaattcgatc
     5161 ttggtagctc caatcgttca ggcacagtat acacccgaaa aaatcgtgaa atcgaatgaa
     5221 gaaaccgggt gggatcggaa agaaggagat ggaaaaggca acaaagacgc tgttgatttc
     5281 aaccaaaaga aatcgagcac cacatatttg cctgcaggcg ttgtttggta cgacttctgg
     5341 accaacgaaa aattcaatgg aggtcaagat gtgaataaag aaacaaccat caatacaatc
     5401 ccagtttaca tcaaggctgg ttccatcctt cccattggtc cccaagtgca atatgccgaa
     5461 gagaagaaat gggacaatct tgaaatccgc gtgtacgaag gagccaatgg cgaattcacg
     5521 ttgtacgaag acgagaacga caattacaac tacgaaaaag gtatgtactc gatcgttaaa
     5581 ttcacttgga acgatgccaa aaaagagctg accattgccc cgcgccaagg ttcgttcccc
     5641 ggaatgttgg ccgaacggaa cttcaacatt gtgaaagtct cggttaaaaa tggtattggc
     5701 aatgattttg aaacagaaat cgtcaaggct gtcaactata ccggagaaaa agtaaccgtt
     5761 aaattataaa taggtataac ctgatgtaaa tgagagatat ctatgaacaa tactaaaaga
     5821 atagttaata tagctatggc gttaatgttt actatataca ttaatggtat agcccaatac
     5881 ccgattattc agaccaagtt cactgccgac ccggcaccta tggtgtacaa cgatacggtt
     5941 tatctgtaca cttcgcacga tgaggatgat gcacaggggt ttaaaatgct cga
//
LOCUS       DAOPUW010000024         5889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_13484_length_5889_cov_3.582962, whole genome shotgun sequence.
ACCESSION   DAOPUW010000024 DAOPUW010000000
VERSION     DAOPUW010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13484_length_5889_cov_3.582962"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(651..1307)
                     /locus_tag="PKJ43_00855"
     CDS             complement(651..1307)
                     /locus_tag="PKJ43_00855"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNZ71134.1"
                     /translation="MKNNKLALTILLLCIAQANLFAQNFGIKAGVNFSNAKITMGNTI
                     MSEDFLWNTGFHIGPIVDFPVSKSFSVETGAIFNTKGYHTNSQTSFLGISLGAKSKTS
                     LTYIDIPITAKLSYPVGNMTVFLNAGPYLGIGISGKTTVEANVSTISGSDEYEVVWGN
                     DDDSDLKRIDYGLSLGAGVRFNSIEIGASYGLGLANLATSEDAKMNHQVIGISLGYMF
                     "
     gene            complement(1383..1814)
                     /locus_tag="PKJ43_00860"
     CDS             complement(1383..1814)
                     /locus_tag="PKJ43_00860"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNZ71135.1"
                     /translation="MEVSEKINWEPEYSIGNQSIDNDHKIIVDAYNDLVDILEQNGSR
                     LKLAEVLSVMTDYVQRHFKREEVYMKTMNYPLFEDHKKMHRDYIVNVAMLNNEMMTTW
                     DHMSIAKQLIHFLRGWWIDHILKKDTEYEKFKESSGNDTAY"
     gene            1990..3420
                     /locus_tag="PKJ43_00865"
     CDS             1990..3420
                     /locus_tag="PKJ43_00865"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNZ71136.1"
                     /translation="MTALIHNVMVNQRNLVRVLGLGYVIIFVVSNIIGSGVYKKIAPM
                     AAELHSAVWILLAWAVAGLITIFGALSNAEVAGLLADTGGEFVYLRKIYNRFFAFIYG
                     WSLFAIIQTATISSLAYVFAQSLNSMLPLPEVFSSLAGFSIGGVFFPFQDFGVKLVAI
                     SMIMLLTVLNISGLKSGAWLSKAILFLVLGGLLTIILFGLTSNVSQAAQPVNFNELTT
                     RSVSLSTFFTAMLAAFWAYQGWVSVGFIGGEIKNPNKNLPKGIVIGVFIILAIYLLIN
                     YTYLKVLTIPQLEGIHQAGNQIAAVEAVRGFWGNGGAMFISLLILITTLGCTNASVLT
                     GARPYFAMARDGLFFRPIGNLNKANVPSNSLLWQGIWASVLVLSGTFDQLTDMVIFAV
                     FIFYGATTLGVFILRRKMPDAHRPYKVWGYPVVPVIYFLFCIALFLNTIITRPREAAI
                     GIVLILLGIPVYFWLNRKGRKPHSCE"
     gene            3567..4217
                     /locus_tag="PKJ43_00870"
     CDS             3567..4217
                     /locus_tag="PKJ43_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71137.1"
                     /translation="MKSIVAISLASMLVFSFSACDLTDIGTTKLGGEESVMSKEGVEL
                     WSSSAVVAGVSDFSGVVTSYEDGISVYEGRAVVKNSYLKQLFSNIPEVKVVGDTLKTD
                     NFQVKQTTNGMELLSGPTAGILVKYDSKVGDKYKTDTKGIERKVVSKSTDNDFPYAFF
                     NIKVIEVEEPTVALKSGGVSKITYWANHRFGLVGIQFTFDDGTTAKFPVYCTEENQ"
     gene            4214..5482
                     /locus_tag="PKJ43_00875"
     CDS             4214..5482
                     /locus_tag="PKJ43_00875"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ71138.1"
                     /translation="MKHLVIQGKKEKIPSHPHHKEYIYRSRMFFLANNLYTFFAVNGL
                     KYYIRKVWLQHKYKIPVPYVLLIDPTTACNLKCIGCWAADYGRKSEISYEKLDQILTE
                     AKKLGIDNISFTGGEPLMRKNDLLKLFKKHRSFPYTIFTNATLIDEEFAAEIARLGNV
                     TFLISVEGYRENTDFRRGKGTFDKVMDSMDLLKKHGIAFGFSICYHSKNYEEVCSDGF
                     LDFLRDKGAWFGWMINYMPIGKGADVSLCLNAEQRKFLKDRIDAYVEKHQFTIVDFSN
                     NGHKAYGCVAAGDGFLHINAAGDVEPCAFFHYSDVNIHKVSLAEALASPFLTHFREQK
                     IISDNPHRPCPIMDVPDALLKITNMEGVCSTHLHCQETPAELYAKTKPLADGWKPVAE
                     KYYQDMSDKEKERFKMVAKYLLYRDSKNNI"
     gene            5498..>5889
                     /locus_tag="PKJ43_00880"
     CDS             5498..>5889
                     /locus_tag="PKJ43_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71139.1"
                     /translation="MTEIKKNWKIVGVFLLFAAIVLAIVSSLTGLKVTLPVFAIFSKY
                     IEAKYFAFFSTNFTDELVLLLFIAGLMLIVFCGNKKSVDSELLRGKAMFRAVLYNSFV
                     LIFSILFLYGQGFFAIMVFNLFSTLLIYL"
BASE COUNT         1567 a         1086 c         1307 g         1929 t
ORIGIN      
        1 gtttcatgat cgttaatttt ttagttaata aatatattta ctgaagggaa taatttccgg
       61 tgttcgtttt ttacccctta aagtttatat attaaaatga taaaaattat gatatttgtc
      121 atgatttgaa ttttattttt tctgaaaaag aggtatttac agagtcgcta tgatattggg
      181 tgtgtgtttc tgcgaatgtg tgatcaatcg gaaaagctgg ggagataaaa attatatagg
      241 atcggttgtt tgaaacatgc ggaatagacg ctttcattcg aaacgatgta aacgggctga
      301 aggcccaaaa taatttagcc cagggcaacc gacgaaggag gggcgccctt ggttaaatgc
      361 caaaagccat aagcgtccgc gatagtatct ttaacgaggt gggaatgttt cttcggacgc
      421 aatagcggtt tgtttacttt ggttggtttt taaacgcaaa ttacaattga ggtgatattc
      481 cgtccgaaac aacgggttgt gcatatcggc agaaccttcc ccagaatctc cgcttgatcc
      541 ctatgttgag taggggggga gtcatctaaa tataaaaaaa gcagcacacg ggctaattgc
      601 ctgcatgctg cttatgtgtt tcgggactaa aggataagcc ctatcagttg ttaaaacata
      661 tatcccagcg agatgcctat aacctgatgg ttcatttttg catcctctga tgttgccaaa
      721 ttggctaaac caagcccata cgatgctcca atttctattg aattaaaccg taccccggca
      781 cccagcgaaa ggccatagtc aatacgttta agatcggaat cgtcgtcgtt gccccaaaca
      841 acttcatact catcgcttcc gcttattgtg cttacgttgg cttctactgt tgttttaccc
      901 gaaataccaa tgcctaaata cgggccggcg ttgagaaaga cagtcatatt accaaccggg
      961 tacgatagtt ttgccgtaat gggtatgtcg atataggtaa gcgaggtttt tgatttggca
     1021 cctaaagata tccccaaaaa cgaagtttga gagttggtat gataaccttt ggtgttgaat
     1081 atggcaccgg tttcaaccga aaacgatttc gaaacaggaa aatcaacgat tggcccaatg
     1141 tgaaaacctg tgttccacag aaaatcttca ctcataatgg tgttccccat ggttattttg
     1201 gcattcgaaa agtttacccc ggcttttatg ccaaaatttt gcgcgaatag atttgcctgg
     1261 gcaatacata ataacaggat tgttaatgct aatttgttgt ttttcatttg attgttattt
     1321 ttgttctctt gtatggttgt gacgcgatta agcaaattgt tatgtctgaa aacgtgcaaa
     1381 agttagtaag cagtatcgtt gcccgatgat tctttgaatt tttcgtattc agtatctttt
     1441 ttcaggatat ggtcaatcca ccaacctctc agaaaatgaa ttagttgctt ggcaattgac
     1501 atgtggtccc atgtcgtcat catttcgttg ttcagcatcg caacatttac aatgtaatcg
     1561 cggtgcattt tcttatgatc ttcaaatagc gggtaattca ttgttttcat gtacacttct
     1621 tcgcgtttga aatgacgctg aacatagtcg gtcattactg aaagcacctc cgccagtttg
     1681 agccgcgatc cgttttgctc aagtatatca acaagatcgt tataagcatc cacaataatt
     1741 ttatggtcat tatcgatgct ctggttgcct attgaatatt ccggttccca gtttattttt
     1801 tcagagactt ccattgtaat attttattat attgaactta acctgcactt accccctgtt
     1861 gaacgtaagg ctaaaaaacc agtagggtaa aactacgcaa atatttcctt ttgttcaatg
     1921 aatttacttc ctttttcttg ttcaagcttt ttgtgttgtc cgcttagttt tctatctaat
     1981 tgtttaaatt tgacagcact tatacataac gttatggtta atcagcgcaa ccttgtcagg
     2041 gtacttggct taggctatgt tattattttt gtggtttcca atataatagg ttcgggtgtt
     2101 tataaaaaga ttgcccctat ggcggctgaa ttgcattcgg cggtttggat tttacttgca
     2161 tgggctgtgg ccgggttaat taccattttt ggggcattga gcaatgccga agttgctggt
     2221 ctgcttgccg atactggtgg cgagtttgtg tatttgcgca aaatttacaa ccgttttttt
     2281 gctttcatat atgggtggtc gttgtttgcg attattcaaa cggccactat ttcgtcgctg
     2341 gcgtatgttt ttgcccaatc attgaacagc atgctaccgt tgccggaagt tttttcgtcg
     2401 ttggccggct tttccattgg cggtgttttt tttccatttc aggattttgg ggtaaagctt
     2461 gtcgccataa gcatgataat gcttcttact gtattgaata ttagtggatt gaagagtggg
     2521 gcgtggctaa gcaaagcaat cttgtttttg gtgttgggcg gactccttac cattattctg
     2581 tttgggctaa ccagtaatgt ttcacaagct gcgcaacctg tcaattttaa cgagctgaca
     2641 acacgtagcg tttcgttatc aacttttttc acagccatgc ttgccgcttt ctgggcatat
     2701 caggggtggg tttctgttgg ctttatcggc ggagaaatta aaaatcccaa taaaaacctt
     2761 cccaaaggga ttgtgattgg ggtgtttata attctggcaa tttatttgct catcaattat
     2821 acttatctga aggttttgac cattccgcaa cttgaaggga ttcatcaggc gggtaaccaa
     2881 attgcggctg ttgaggctgt acggggtttt tggggcaatg gaggggccat gtttatttcc
     2941 ttactgattt tgattaccac tttgggctgc accaatgcca gtgtgttgac gggggccagg
     3001 ccttattttg cgatggcacg cgatggcttg tttttccggc cgataggaaa tcttaataaa
     3061 gccaatgtac cgtcaaattc gttgctctgg caaggcattt gggcatcggt gttggtgttg
     3121 tcgggaactt tcgaccaatt gacagatatg gtgatttttg ccgtatttat tttttacgga
     3181 gccacaacat taggcgtatt tatcttgcgc cgaaagatgc ccgatgcaca tcgtccgtac
     3241 aaggtttggg gctatcccgt tgttcctgtt atttatttct tgttttgtat cgcgctgttt
     3301 cttaacacca taattacccg tccgcgagaa gcagctattg gcattgtgtt gattttgctg
     3361 ggtattccgg tttatttttg gctaaacagg aaaggccgca aaccgcattc ttgcgagtga
     3421 tgctgtaatg gcttatttat taaatgaata tgtttcggtt tttttcgagt cccttccatt
     3481 tctagtaaat aaaagttgta tatttgagtg tagaatacat cacaatgtgt tttataactg
     3541 tagtattaat ttaaaataaa tcggacatga aatcaattgt ggcaattagt ttagcatcca
     3601 tgctggtgtt ttcgttttcg gcatgtgatt taaccgacat tggaaccacc aagttgggag
     3661 gagaagagtc tgtgatgagc aaagaaggtg ttgaattatg gtcgtcgtcg gctgtagttg
     3721 ccggggtttc tgatttctca ggagtagtta cctcttatga agatggcatt tcggtatacg
     3781 aaggtagagc tgttgtgaaa aatagctatt tgaagcaact gttctcaaat attcctgaag
     3841 ttaaggtagt tggagataca ttgaaaaccg ataatttcca ggtaaagcaa acaaccaatg
     3901 gcatggagtt gctttcgggg ccaacagccg gaattttggt caagtatgat tcaaaagttg
     3961 gcgataaata caaaaccgac accaaaggca ttgagcgtaa ggtggtaagt aaatcgacag
     4021 acaacgattt tccgtatgct tttttcaata ttaaggtaat tgaagttgaa gaaccaacag
     4081 tggcgcttaa aagtggtggc gttagcaaga ttacttattg ggccaatcat aggtttggat
     4141 tagtggggat tcaatttact ttcgacgacg gaacaaccgc aaaatttccg gtttactgca
     4201 ctgaggaaaa tcaatgaagc atttagtaat tcaagggaaa aaggagaaaa taccttcaca
     4261 tccacatcat aaagagtaca tttaccggag ccggatgttt tttctggcta ataatctata
     4321 tacatttttt gccgtcaatg ggcttaagta ttacatccgg aaagtgtggc ttcaacacaa
     4381 atataagata ccggttccct atgttcttct tatcgacccc actacagcat gtaatctgaa
     4441 atgcattggt tgctgggctg ctgattatgg acggaaaagt gaaatttcgt acgagaaatt
     4501 ggatcagata cttactgagg ccaagaagct tggaatcgat aatatttcat ttaccggagg
     4561 agaaccattg atgcggaaaa acgatttgct gaaactattc aagaagcacc gttcgttccc
     4621 ttatactatt ttcaccaatg ccacgcttat tgacgaagaa tttgcagccg aaatagcccg
     4681 gctgggaaat gtaacctttt tgatcagcgt ggaaggttat cgcgaaaata ccgacttcag
     4741 aaggggaaaa ggtactttcg acaaagtgat ggacagcatg gatttgctta aaaaacacgg
     4801 gattgctttt ggattttcaa tctgttatca ttcaaaaaac tacgaagagg tttgcagtga
     4861 tggctttctc gattttttgc gtgacaaagg tgcctggttt ggttggatga tcaattatat
     4921 gcccattggg aaaggtgccg atgtttcgtt gtgtctcaat gctgaacagc gtaagttctt
     4981 aaaagaccgg attgatgcgt atgttgaaaa acaccagttt actattgtcg atttctctaa
     5041 caacggccat aaagcgtatg gttgcgtggc ggctggcgat ggctttttgc acattaatgc
     5101 ggccggagat gttgagccct gtgccttttt ccattattcc gatgtaaata ttcataaagt
     5161 ttccctggca gaggcgctgg cttcaccgtt tcttacgcat ttcagagagc aaaaaataat
     5221 ttcggacaac ccccacaggc catgccctat tatggatgtg cccgatgcgc tgctgaaaat
     5281 taccaatatg gaaggtgtct gttctactca cttgcattgc caggagaccc ccgccgaatt
     5341 gtatgcaaaa accaaacccc ttgccgacgg gtggaaacct gtggccgaaa aatattatca
     5401 ggatatgtcg gataaagaga aagaacggtt caaaatggtc gcgaaatatt tgctttacag
     5461 ggattctaaa aacaatattt aattgaaatt tctttgtatg actgaaatta aaaaaaactg
     5521 gaaaattgtt ggtgtatttt tactctttgc cgcgattgtt ttggctattg tttcttcact
     5581 taccggctta aaagtaacac ttcctgtttt tgcaatcttt tctaaatata ttgaagcaaa
     5641 gtattttgca ttcttctcaa caaatttcac tgacgagttg gtgctgctgc tatttatagc
     5701 tggcttgatg ctgatcgtat tttgtggaaa caaaaaaagc gttgatagcg agctccttcg
     5761 tggtaaagca atgtttcgcg ctgtgctata taactcattt gtcctgattt tttcaatact
     5821 tttcctctac ggtcaggggt tctttgcaat tatggttttc aatttgtttt caacattgct
     5881 gatctatct
//
LOCUS       DAOPUW010000025         5885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_13495_length_5885_cov_3.797599, whole genome shotgun sequence.
ACCESSION   DAOPUW010000025 DAOPUW010000000
VERSION     DAOPUW010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13495_length_5885_cov_3.797599"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PKJ43_00885"
     CDS             <1..447
                     /locus_tag="PKJ43_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71140.1"
                     /translation="AERGSFLIEDNSLLTTVSWTGGNDQLLFVKSEDEEEQAWNDDFI
                     HMEGDFSVSYTLPRIVQGSYWLYIRANAFNVDNALIEVFFDGVKVGGLIDLTTGGNAQ
                     YPYVEFELGEVTLLDYKPHVITVTSLIPGYFDWDYVYFRPTYLEKK"
     gene            444..1991
                     /locus_tag="PKJ43_00890"
     CDS             444..1991
                     /locus_tag="PKJ43_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNZ71141.1"
                     /translation="MKKQLVYIMLFCAVTLFTACENTNWDEHYNNVPETVDMNVWDAM
                     QQNSDISQFVQLVKEHKLDTLFNYTDVYTLFAPTNAQMSAFLASDTIGKTGLAYHFLK
                     YYINPENITGKRKVQSLFLKFAQFENTGDSYLYDNFPVTFSSPLYKNGRYFVIEGVAK
                     PKPSLYEYISANNLALKKFIDDQDSIALDKELSKPIGYNSEGKTIYDSVITVINLFEE
                     EYYAVSEEYRVKTATLVFPNQERYNEGLTGMALKLGGDYNSYEDIPEDWQQEVLIPYL
                     INNGIFRNMLEPKDFLSDTMLNIIGDTATILYNPVDLTVCSNGYAYNYDKFEVADTLF
                     MSSARTEGESLLNTKGADIFEWSDSVTVSDIKIQPDADFVKGSSNDSILKVQLPTGSK
                     DEYWIDFKSNPLFPRRYLMLFRTHMDFGGIFEIYVNDVLVKTFDWADFAKNRGIIPSV
                     VPGVRHIAVGRYCKFDCWVDNITQYGKAQIRIVYKGPSTMRYNGLMLDYIEFVPENRV
                     GSITKNP"
     gene            2132..5329
                     /locus_tag="PKJ43_00895"
     CDS             2132..5329
                     /locus_tag="PKJ43_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNZ71142.1"
                     /translation="MNNFKTSSAMDINPTFRILTRTLLVITMFIIGSQLLHAQETKTV
                     NGIVISPESKPVPNVSVSVEGSRDMPAFTDGEGKFSINLGENGAWINVAPAADYKKKR
                     VFVGKRNELKIMLTPIDIAGSDDEIILLNQPVAVKNVVSSFSSVNTENIHHTAVTSID
                     NYMQGRVAGMHVLNQSGAPGSGAVTQLRGVNSINATNQPLYVVDGAIMEPTGLFGTIV
                     NGYNYNPLLSINPLDISNTNVMKDPVYQAAYGSKASNGLVMIQTLDPSATETSFDIDL
                     RRGLTLKPEQYIPQLDANQYKTLANEILFSSGAVEEDIIEKFPNLFLEPEDLRFIDYQ
                     HNTNWQQHVFENSSFTNFNVKVKGGDEIARYGLSFGYYNSSGILKNTSYDGYNIRFVS
                     LVNIFTWLRMNAAVSFNTSNMVLKESALVEETSPILSALSKSPLLNPYQYDAEGVETR
                     LLSEVDEFGVSNPLATINNFDASSSNYHITTSLGFEADLGENLFLRTNVGILYNSLKE
                     RMFMPNKGMESYYDDEAYNVAKASANVYTGFSNNTMLVYNKKIDSDHAINSTTGLNIM
                     TNQFQYDFGVAKNAHENDEYRMLADGRDNLREVGGSTHNWNWMSIYEKISYVYQDKYL
                     FTGTVSLDGSSRVGRDADKTLNLFGQPFGIFYSAGLGWRVSNEGFLDDISWLDEFKIR
                     ASIGRTGNDDIGETNATNFYQSLRYRESTGIVPSTFPNYKLTYEFNTQYNIGADVALW
                     GNRVKATFDFFKTDISDMLVYMPLDVYLGYNFRPENAGAMENTGWDATLFIRLVNARS
                     FKWDIETTLSHVQNSVTKMPHDKFVTSFNNYELVNMVGEQVNSFYGYRFNGVYATSDD
                     AAAAGLVNERFKPYGAGDAIFEDISGPDGKPDGQINSYDKTTIGSPLPEYIGGIVNTF
                     RYKNWTLSAYVNFVTGNEVYNYLRFKNEAMVDFSNQSKNVLNRWQYENQSTDVPRALW
                     NDPIGNSAFSTRWIEDGSFLRLKNISLSYKIPHEFLAFKSAEFYVSASNLFTFSKYLG
                     YDPEFAYSYHLNQQGIDFGQIPQSRQFLVGIKIGL"
     gene            5340..>5885
                     /locus_tag="PKJ43_00900"
     CDS             5340..>5885
                     /locus_tag="PKJ43_00900"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNZ71143.1"
                     /translation="MNKYIQSAILFLSALLLAGVLFSCEDFFNPEQGVTVTEDQLYTD
                     WYEYRAVALGMYGLQQQLVEQLMVLGELRGDLLTVTDNADEDLMEIYNFNVSKDNKYA
                     SPQNFFKLIAATNRLISILEERQPRVTNLALTEVNNYDKLYGEALCMRAWAYFNAARI
                     YGKVPFIDQRLSTIEEIEQFIN"
BASE COUNT         1751 a         1095 c         1259 g         1780 t
ORIGIN      
        1 gccgagcgag gatcgttcct cattgaagat aattctttac tcactacagt gagctggaca
       61 ggaggcaacg atcagttgtt gttcgttaaa tcggaagatg aagaagagca ggcatggaat
      121 gacgacttta ttcacatgga aggtgatttc tctgtgagtt atactttgcc ccggatcgtt
      181 cagggaagct attggctata cattcgggct aatgccttta atgttgacaa tgctctgatt
      241 gaagtatttt ttgatggagt gaaagtaggt ggtcttatcg accttaccac tggtgggaac
      301 gctcaatatc cttatgtcga gttcgaattg ggtgaagtta ctctgctcga ttacaaaccg
      361 catgttatta cggttacctc gctaattccc ggatatttcg attgggatta cgtttatttc
      421 agaccaactt atcttgaaaa gaaatgaaga agcagttagt ttatatcatg ttattttgtg
      481 ctgtaacgtt gtttactgcc tgtgaaaata caaattggga cgaacattat aacaacgtgc
      541 ccgaaactgt cgatatgaat gtttgggatg ctatgcagca aaatagcgat atttctcagt
      601 ttgttcagtt ggttaaagaa cataagcttg ataccttgtt caactatacc gatgtgtaca
      661 cgctttttgc tccaacaaat gcccagatgt cggccttcct tgcttccgat accattggaa
      721 agactgggtt ggcatatcat tttttgaaat attatattaa tcctgagaat attaccggaa
      781 agcgcaaggt gcagtctctt ttccttaagt ttgctcagtt tgaaaatact ggcgacagtt
      841 atctctacga taattttccg gttacatttt caagcccgct atacaaaaac ggacgatact
      901 ttgttattga aggcgttgca aaaccaaagc caagccttta tgaatatatt tcagccaata
      961 atttggcctt gaaaaaattc atcgatgatc aagactctat cgctttagat aaagaactca
     1021 gtaagccaat tggatacaat agtgaaggaa aaactattta cgattcagta atcacggtta
     1081 tcaacctctt cgaggaagaa tactacgctg tttcggagga gtacagggtg aaaacggcca
     1141 cgttggtttt tcctaatcag gaacgttata acgaaggtct taccgggatg gctcttaaac
     1201 ttggtggcga ttataattca tatgaagaca tacctgaaga ctggcagcag gaagtattaa
     1261 ttccatatct tatcaacaac ggtattttca gaaatatgct tgaacccaaa gattttcttt
     1321 ctgataccat gttgaatatt attggcgata ctgctacaat attgtataat cctgtcgatt
     1381 tgactgtttg cagtaatggt tatgcttata attacgacaa atttgaagtc gccgatacgc
     1441 ttttcatgtc atcggcacgt actgaagggg aaagtctttt gaatacaaaa ggcgccgata
     1501 ttttcgaatg gagtgacagt gttacagtga gtgatattaa gatccagccc gatgccgact
     1561 ttgtgaaagg atcatcaaat gattctattc ttaaagtcca attacctact ggatcgaaag
     1621 acgaatactg gatcgatttt aaatcaaatc cgttgttccc aaggcgttat ttaatgcttt
     1681 tcagaacaca catggacttt ggcggcattt tcgagattta tgttaacgat gtgctggtaa
     1741 aaacttttga ttgggccgat tttgcaaaaa acagaggtat tattcctagt gttgtcccag
     1801 gggttaggca cattgcagta gggcggtatt gtaaattcga ctgttgggta gataacatta
     1861 cccaatatgg taaagcccaa atacggatag tatacaaagg tcctagcaca atgagataca
     1921 atgggctgat gctggattac attgagttcg tgccagaaaa tagagtaggg tcaattacaa
     1981 agaatccttg atgttttcat ttttcagagg atgagtcagg tctttaaaat gaatgttaat
     2041 ggcgaagaaa aataaaaata caatcttacc ctgttgttat ggagcagaaa aaaacctgtt
     2101 cacaacaaga gattgcaaga cgataaacga tatgaacaac tttaagacaa gttcagcgat
     2161 ggatattaat cctacatttc gaatactaac ccgtaccctg ttggtaataa ctatgtttat
     2221 aattgggtcc caattgttgc atgcacagga aacaaaaacg gtgaatggca ttgttatttc
     2281 gcccgagagc aaaccggtac cgaacgtttc ggttagtgtt gaaggctcgc gcgatatgcc
     2341 tgcttttacc gatggagaag gtaaattttc gattaacctt ggagagaatg gtgcttggat
     2401 taatgttgct ccggctgccg actacaagaa aaaacgtgtt tttgtaggta aacgcaatga
     2461 actgaaaata atgttgactc ctattgatat tgcagggagc gacgacgaaa tcattttact
     2521 gaaccaacct gtcgcagtga aaaatgttgt atcgtcattt tcaagcgtca atacagaaaa
     2581 tatacaccat actgcggtca cttccatcga taactatatg caaggccgtg tggcgggtat
     2641 gcatgtgttg aatcaatctg gagctcctgg cagtggtgct gtaacacaac tccggggcgt
     2701 aaattcgatt aatgctacaa accagcctct ttatgtagtc gatggggcta ttatggaacc
     2761 taccggtcta tttggtacta tcgtcaatgg ctataattat aacccgcttt tgtccatcaa
     2821 tccgctcgac atttcgaata cgaatgttat gaaagacccg gtataccaag ccgcatatgg
     2881 ctctaaagca tcgaatggtt tggtaatgat tcaaactctc gatcccagtg ctacagaaac
     2941 atctttcgat atagaccttc gccggggact tacgttgaaa ccagaacagt atattccgca
     3001 actcgatgca aaccaataca agactcttgc caacgaaatt cttttttctt caggagccgt
     3061 tgaagaagat ataattgaga agtttccaaa cctttttctc gaaccagaag atttgcggtt
     3121 tatcgactat cagcataata ccaattggca acaacatgtg ttcgaaaact catcgtttac
     3181 caatttcaat gttaaagtta aaggaggtga cgagattgcc cgttacgggt tatcttttgg
     3241 gtactataac tcgtcaggta tccttaaaaa cactagctac gacggctata atatccgttt
     3301 tgtgagttta gtaaatattt tcacatggct tcgtatgaat gccgcagtgt cgttcaatac
     3361 ctctaacatg gtgctgaaag aatcggcact tgttgaagaa acaagtccaa ttttgtctgc
     3421 cctttctaag tcacctttgc tgaaccctta tcagtacgac gctgaagggg ttgaaactcg
     3481 gttattaagt gaagttgacg agtttggtgt tagtaaccca ttggcaacca tcaataattt
     3541 cgatgcatcg agcagtaact atcatattac cacttcactt ggttttgaag ccgatctggg
     3601 tgaaaatcta ttcttgcgca ccaatgtggg gattttatat aattcactga aagagagaat
     3661 gttcatgcca aataagggaa tggagtctta ttacgacgat gaagcctata atgtggctaa
     3721 agcttcggct aatgtgtaca ctggtttttc gaacaacacg atgcttgttt acaataaaaa
     3781 aattgattct gaccatgcca tcaattctac aactggattg aatattatga ccaatcagtt
     3841 tcagtacgac tttggggtag caaaaaatgc ccacgaaaat gatgagtacc gcatgctggc
     3901 tgatggtcgc gacaatcttc gcgaagttgg ggggtcgact cataattgga actggatgtc
     3961 gatttacgaa aaaatatcgt atgtctatca agacaaatac ttgtttactg gtacagtttc
     4021 actcgacggt tcttcaagag ttggtcgtga tgctgataaa acgttgaact tgtttggcca
     4081 accttttggg attttctact cagccggttt gggatggagg gtgtcgaatg aaggcttcct
     4141 tgatgatatt agctggctcg atgaatttaa aatcagagct tctattggtc gtacaggaaa
     4201 cgacgatatt ggcgaaacca atgcaactaa tttctatcag tcgttacggt atcgtgaatc
     4261 aacgggtatt gtgccttcta cttttcccaa ctataaactt acgtatgagt ttaatacgca
     4321 atacaatatc ggagcagatg tcgctctgtg gggaaatagg gtgaaggcga cattcgactt
     4381 ttttaaaaca gacatcagcg acatgttggt gtatatgcct ctcgacgtgt acctagggta
     4441 caatttcaga cctgaaaatg caggagccat ggaaaacact ggttgggatg ccaccctttt
     4501 catccgtttg gtaaatgctc gtagttttaa gtgggatatt gaaaccacct tgtcgcatgt
     4561 tcaaaattca gtaaccaaaa tgcctcatga taaatttgtc acttcattca ataactatga
     4621 gttggtaaat atggttggtg agcaggtaaa cagcttctat ggttatcggt tcaatggggt
     4681 atatgctaca agtgacgatg ctgctgcagc gggtttggtt aacgaaagat ttaaacctta
     4741 cggagcgggt gatgcgatct ttgaagatat atcgggtcct gatggtaaac ccgacggtca
     4801 aatcaacagc tacgacaaaa ctacaatagg ttcaccactt cctgaataca ttgggggtat
     4861 tgtgaacact ttccgttaca aaaactggac attaagtgca tacgttaatt ttgtaactgg
     4921 caatgaagtg tataactact tgcgctttaa aaacgaagcc atggttgatt tctcaaacca
     4981 aagcaaaaat gtgctgaatc gttggcaata tgaaaatcag tcaaccgatg tgcctcgtgc
     5041 tttatggaat gacccaatcg ggaacagtgc attttcaacc cgctggattg aagatggttc
     5101 atttttgcgt ctgaaaaata tttctctcag ttataaaatt ccacatgaat ttcttgcatt
     5161 taaaagtgca gagttctatg tttcagcttc taacttgttt accttttcca aatatttagg
     5221 atacgatccc gagtttgcat actcgtatca tttgaatcaa caagggattg actttggtca
     5281 gattcctcag tcaagacaat ttttagtggg tattaaaatc ggattataac aatagtatta
     5341 tgaataagta tatacaatca gcgatattat tcctttcggc acttctttta gctggtgttt
     5401 tattctcctg tgaagatttc tttaatcctg aacagggggt aacagttaca gaagatcagc
     5461 tatataccga ttggtatgaa tacagagcag tggcattggg catgtatggt cttcagcagc
     5521 agttggttga gcagcttatg gtgcttggtg aactcagggg cgatttgctt actgttaccg
     5581 ataatgccga tgaagattta atggagatat acaattttaa tgtgtcgaag gataataaat
     5641 atgcatcgcc acaaaatttc ttcaaactta tcgctgctac aaaccgcttg atcagcattc
     5701 ttgaagagcg gcaaccccgt gttaccaacc ttgcccttac cgaagtaaac aattacgaca
     5761 aactatacgg cgaagcatta tgtatgaggg cttgggccta ttttaatgcg gctcgaattt
     5821 atggtaaagt accttttatc gaccaacgcc tttctacaat tgaagaaatt gaacaattca
     5881 taaat
//
LOCUS       DAOPUW010000026         5749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_13920_length_5749_cov_4.129434, whole genome shotgun sequence.
ACCESSION   DAOPUW010000026 DAOPUW010000000
VERSION     DAOPUW010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13920_length_5749_cov_4.129434"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1466)
                     /locus_tag="PKJ43_00905"
     CDS             complement(<1..>1466)
                     /locus_tag="PKJ43_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ71144.1"
                     /translation="VEANENSGGKPLDFVKTVPNWDKIESGEWKGTNWFDEMLTDNAQ
                     VQNHSLSIIGGSERSTYSMGFGYHDEVSILGPQGNNKYKRLSARLNSEHIVIQSDGRS
                     ILKVGENLSYTNTKNPTFRTGNIYWNDLHNALVANPFLPMYAEDETDKAYPYHYAIAW
                     NSADVNPIALLEYNGKYNTNSNNSILGNVYAELEPIKNLKLRSSYGITAGFGSSRQFT
                     PPYELATRTVVAQDQVTQRMYQNFTWTWTNTASYSKSIGSHNLTAVVGNEMMRNSVNL
                     QLSGKNEKSLFGDFEHAYLYNVTAIDPTYTTLSGRDDYGESLMSYFGRLSYDINETYL
                     FTAVLRADGSSNFDEGHRWGTFPSLSAGWVVSNESFMQNVPFINFLKVRASWGENGNK
                     NIDKFQYLSSLSYDVAAYYFGPDKTIQSNGAYPARLPNPNVSWETSEQISAGFDLHIV
                     DSRLQLVFDWYQKNTRDWFVLVLAFATNGTAVLYINGG"
     assembly_gap    1467..1566
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1567..2444)
                     /locus_tag="PKJ43_00910"
     CDS             complement(<1567..2444)
                     /locus_tag="PKJ43_00910"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNZ71145.1"
                     /translation="MISDLFKQLNQRNFIRSHLFLILLLAFQVAAFAQGRMVSGTVTD
                     ASNEPLPGVNVLIKGTTVGTITDINGKYSIGVSKTNDILVFSFIGYNEHSVVVDNNAV
                     IDVILKEDILQLNEVVAIGYGVQKKKLSTGATLNVKGEDIQKLNTTRPIDALQGLSPG
                     VSIQQSNGLPGSEARVYIRGIGTIGHAKPLYVIDGVSADNIDHIAPSEIESIDILKDA
                     ASAAIYGSRAANGVILVTTKRGSENQKPMISYDFYNGWQSVYKDPGLLNAQQYRDIFV
                     EANENSGGKPLDFVKTV"
     gene            3263..3796
                     /locus_tag="PKJ43_00915"
     CDS             3263..3796
                     /locus_tag="PKJ43_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HNZ71146.1"
                     /translation="MGNPLINHFMEGTKKGTDWFKTIFDEYYDYIRNYLFYLAGDIEI
                     AEDLAQDVFMKLWENRDKVNESTLKPFLFKIAKNLFLNLHKRKKLNLEFVNSRLENIE
                     NESPQFVLEVKEFDEKLQRALSNLPEQCRTYFLLNRIDDMKYQDIAKSFGVSVKAVEK
                     QISKAIKLLREQFDQKL"
     gene            3808..4665
                     /locus_tag="PKJ43_00920"
     CDS             3808..4665
                     /locus_tag="PKJ43_00920"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNZ71147.1"
                     /translation="MKENQDLNIIQFTQDLRVPATISKEEAWQRLEKNISNLPEKPNT
                     QTRFPIYTFISLSIAASILLFIFYFALKQAPTKTFFFETQSSEVKKEILPDSSIIFLN
                     ANSTATFTYNKFRKKREVSLSGDAFFNVRKGKEFSVLFGGGQVNVLGTAFYVSAYSDR
                     FISIECTEGKVWVEIDNQKVTLNAGEGIKSFDHILSEKFTVNEKDVKEKLNGVFFWQK
                     ISLAEIEELIENRLGYNVILETTLATRNFSGELLLTTPQSFLPTLAAAMNLDYTINDK
                     EKTITLNAK"
     gene            4655..>5749
                     /locus_tag="PKJ43_00925"
     CDS             4655..>5749
                     /locus_tag="PKJ43_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71148.1"
                     /translation="MPNKLRQYLLFTCVLLIFSIQGSAQKLISYKATNEPLNAVLAKV
                     ATQGNVRFAFDDDYMSRIKVTFDANNIQVEDFLKLISGKYKISYRLIGNSWVLAGEEK
                     TSVSANQQKKENQPKKIAKVTEKKPAKVNRLWNFMGIIIDSRTGNRLLNATIYIDNLR
                     KTKTNTAGFFEEEVSSAGDLQIKLNHWGYHPLDTTILLNDTVDISLKVVPLLIAGQLP
                     GSSYSDFPVYPSTASGAFSMNLSAGTNPGGKDITDYFNYFGLIPEVTLQMETGLEVRE
                     SSYAPENIYIDGIPLMNLSHLNGQLSPVNAQYIHQGSVSRGGLSSAYANNSSGYIDLI
                     GRNGFVKPMTETTFTPLNTSLYIGIPITPKV"
BASE COUNT         1839 a         1163 c         1024 g         1623 t
ORIGIN      
        1 acctccattg atataaagaa cggctgtacc attggttgca aatgccaaaa caagaacaaa
       61 ccaatcacga gtattttttt ggtaccagtc aaaaaccaat tgtaagcggc tatcaacaat
      121 atgcaagtcg aaacctgcgc tgatttgctc tgatgtttcc cagctgacat tggggtttgg
      181 taatcgtgct ggataagccc cattgctctg aattgtcttg tctgggccaa agtaataagc
      241 tgccacatcg tatgaaagag acgataaata ttgaaattta tcaatgtttt tatttccatt
      301 ctctccccag ctagccctta ctttaaggaa gttaatgaaa ggaacatttt gcataaacga
      361 ctcgttgctt accacccaac ctgccgataa cgacgggaaa gttccccaac gatgtccttc
      421 gtcaaaattg gaagaaccat cggcacgtaa cacagctgta aataaatagg tttcattgat
      481 atcgtaagat aaacggccaa aatacgacat gagtgattct ccgtagtcat cacggccact
      541 aagtgttgta tacgttgggt caatagcagt tacattatat aaataagcat gttcaaaatc
      601 tccgaaaagc gatttttcgt ttttcccact tagctgaagg tttaccgaat tgcgcatcat
      661 ctcgttaccg acaacagcag tgagattgtg actaccaata gatttactgt agctggcagt
      721 atttgtccag gtccaggtga aattttggta catacgctga gtcacctgat cttgagcaac
      781 aactgtccgg gttgcaagtt catacggagg agtaaactgc cgtgatgaac caaaaccagc
      841 agtaatacca taagaagaac ggagcttcaa atttttaatt ggctctaatt cagcatatac
      901 atttccaagt atcgagttgt ttgaattggt attgtatttt ccattatact caagcagagc
      961 gattgggttc acatcagctg aattccaagc gattgcataa tgatatggat aagccttatc
     1021 ggtttcatct tctgcataca ttggaaggaa tgggttagcg accagtgcat tatgcaaatc
     1081 attccaatat atattaccgg ttctgaatgt cgggttcttt gtgttggtat acgaaagatt
     1141 ttccccaact tttaaaatac tcctaccatc agattgaata acaatatgtt ctgaattcaa
     1201 tcgggcagaa agtcttttgt atttattatt tccttgtggt cctaaaatac taacttcatc
     1261 gtggtaacca aatcccatag aataagttga acgttcagaa ccaccaataa tcgacaatga
     1321 gtggttttgc acttgtgcat tatcggtaag catttcgtca aaccaattgg tacctttcca
     1381 ttcaccgctt tcaattttat cccaattagg aacagtttta acaaaatcga gcggttttcc
     1441 gccactattt tcgttagcct ctacaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnacag ttttaacaaa atcgagcggt tttccgccac tattttcgtt agcctctaca
     1621 aaaatgtcac gatattgctg agcattaagc aaacccggat ctttatacac actttgccat
     1681 ccattgtaaa aatcgtaaga aatcattggt ttctggtttt cgcttccccg tttggtagta
     1741 acaaggatta ccccattggc agcacgtgaa ccataaattg cagcagatgc tgcatctttg
     1801 agaatgtcaa tcgattcgat ttcggaaggt gcaatatggt cgatattatc ggcagaaaca
     1861 ccatcaatta catagagtgg tttagcatgg ccaatagttc caatcccacg gatatataca
     1921 cgtgcttcag aaccgggaag accattgctt tgctgaatac ttacccccgg gctcaacccc
     1981 tgaagcgcat caattggacg ggtagtgttt agcttctgaa tatcctcgcc tttcacatta
     2041 agcgtggcac cggtgctgag ttttttcttc tgtacaccat aaccaatagc tacaacttcg
     2101 ttaagctgaa ggatatcttc tttcaaaata acatcgataa ccgcgttatt gtcaacaact
     2161 acactatgct cgttgtagcc aataaacgaa aaaacaagaa tatcgttagt tttactcact
     2221 ccgatagaat attttccatt gatatcggtt atggttccaa cggtagttcc tttaattaac
     2281 acattaaccc cgggaagagg ctcgtttgac gcatcggtaa ctgtacctga caccattcgg
     2341 ccttgtgcaa atgctgcaac ctgaaaagcc aacaatagta tcagaaagag atgagaacgt
     2401 ataaagttcc gctgattcaa ttgtttaaat agatctgaaa tcataggtct aagattaatt
     2461 ttattattag aatttgtccg gtaaatatag gcattaaata aaatgacaag gggtaaaaat
     2521 tgctcaatta aggggctaat atgtttatca aagatacatt ttttataaaa aaaggcaccc
     2581 aattgaggtg ccacgtgttt tacatcatta tttctgtcta aaatttctac cagccggggt
     2641 attgaaccag attgttgttt gagttgatta tttcagattc agggatcgga aaaaaatcgt
     2701 tttgccccga cttgaacgag ctttcaatgg gcgtttgagc tacgcctctc aaatattttt
     2761 atgattgatg cctttcttcg ggaactgatt gacaattccg atgtacaacg tagaatttga
     2821 gattgcggaa taataatacg gggataaaat ttaaaaatat gtgggtctaa taaaacaagt
     2881 tcttaattaa aacgcaacac tcaaatctta tttatcattg aatattaaat aattaaagat
     2941 ggtcaggaag tagtaaatat aatattttac agtagaagtt gtgaagaaag cattaaggta
     3001 tttgcccttc cccgaagcat ctaagcccct aaaaaaagca aattcaattt ctcaattaaa
     3061 acagctgttt gccttgataa aataataata atgggactcc gtttacaata ttgttttatt
     3121 ttttagataa aatagagtta aacaaataat taattagcaa ccatttgttg ttaattatta
     3181 aaaaaaactg ccaggataaa gccaagcatt atttcttgct ttgtgaaaat ttgtagcttt
     3241 gtgtggcttg aacgtctaat gtatgggaaa tccccttata aatcatttta tggaagggac
     3301 aaaaaaagga acagattggt ttaaaacaat ttttgatgaa tattacgatt acatcagaaa
     3361 ttatcttttt tacttagccg gagacattga aattgccgaa gacttagctc aggatgtttt
     3421 catgaaactt tgggaaaacc gcgacaaagt aaatgaaagt acgcttaagc cattcttgtt
     3481 taagatagcc aaaaaccttt ttttaaacct ccataagcga aagaaactca accttgagtt
     3541 tgtaaactcc cggctcgaaa acattgaaaa cgagtcgcca cagtttgtat tggaagtaaa
     3601 agagtttgac gaaaaacttc aacgcgccct gtcgaatttg cccgaacaat gccgcaccta
     3661 cttcttgctc aaccgcatcg acgacatgaa ataccaagac atagccaaaa gttttggcgt
     3721 tagtgtcaaa gctgtagaaa aacaaattag caaagcaata aaacttcttc gcgagcaatt
     3781 cgatcaaaaa ttgtaataat tgttgagatg aaggaaaatc aggatttaaa tattatccaa
     3841 ttcactcaag atttgcgcgt tcctgcaaca atttcgaaag aagaagcctg gcaacgtctg
     3901 gaaaaaaaca tttccaacct tcccgaaaaa cccaacactc aaactcgatt ccccatctat
     3961 acatttattt ctctctctat tgcagcctct atcctcctct tcatctttta cttcgcctta
     4021 aaacaagccc caacaaaaac cttttttttt gaaacccaat cctccgaagt caagaaagaa
     4081 attttacccg atagtagcat catcttctta aacgctaact caacagccac tttcacatat
     4141 aacaaattca gaaaaaagcg tgaagtaagc cttagcggtg atgccttttt taatgtcaga
     4201 aaaggaaaag aattttcagt tctcttcgga ggcgggcaag ttaacgtact tggtactgca
     4261 ttctacgtat cggcatattc cgatcgtttc atctccattg aatgtaccga aggaaaagtc
     4321 tgggttgaaa ttgacaacca gaaagttacc ctaaatgctg gcgaaggaat aaagagtttt
     4381 gatcacattc tctcggaaaa atttacggtc aacgaaaagg atgtaaaaga aaaacttaac
     4441 ggcgtatttt tttggcagaa gatatcgtta gccgaaatag aagaacttat tgaaaacagg
     4501 cttggttaca atgttatttt agaaacaacc cttgccacaa gaaatttctc aggagaatta
     4561 cttcttacca ccccgcaaag ctttctgccc actcttgctg cagctatgaa tctcgactac
     4621 actatcaacg acaaagaaaa aacgatcacg ttaaatgcca aataaactca ggcaatatct
     4681 tttgtttacc tgcgtactgc tcatcttttc aattcaggga tcagcacaaa aactcatttc
     4741 atacaaggca accaatgagc cgcttaacgc tgtgctagca aaagttgcta cacaaggtaa
     4801 tgtaaggttt gccttcgacg acgactatat gtcgagaata aaagtaacct tcgatgccaa
     4861 caatattcag gtagaagatt ttctaaaact aatttcaggc aaatacaaaa tttcatatcg
     4921 ccttataggt aattcctggg ttctagcggg tgaagaaaaa actagcgttt ctgctaacca
     4981 gcaaaaaaaa gaaaaccaac ctaaaaaaat agcgaaagtc acagaaaaaa agcctgcaaa
     5041 agtaaaccgg ctttggaact ttatgggaat cataatcgac tcacgtacag gtaaccgact
     5101 gctaaatgcc accatttaca tcgataattt aagaaaaaca aaaaccaaca ccgctggatt
     5161 ttttgaagaa gaagttagca gtgcaggaga ccttcagata aaactgaacc attggggata
     5221 tcacccccta gacactacaa tacttctcaa cgacactgtt gatatttcat taaaagttgt
     5281 acctctgctc attgcgggcc aattgccggg cagctcttat tccgatttcc ctgtttaccc
     5341 gagcactgcc agcggcgctt tttcaatgaa ccttagcgct ggaaccaacc ctggaggcaa
     5401 agatattacc gattatttca actacttcgg gctaatacca gaggttaccc tgcaaatgga
     5461 aaccggcctc gaagtgcgtg aaagttccta tgcccccgaa aatatttata tcgacgggat
     5521 acctctaatg aacctctccc acttgaatgg ccaattgagt ccggtgaatg cacaatatat
     5581 tcatcaagga tcggtgtcca gaggcggact gagctctgcc tatgccaata acagttccgg
     5641 ctacatcgat ctgatcggaa gaaacggttt tgtaaaacca atgacagaaa cgactttcac
     5701 tccgctgaat accagcttgt atattggaat tccaataacc cccaaagtt
//
LOCUS       DAOPUW010000027         5557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_14587_length_5557_cov_3.287714, whole genome shotgun sequence.
ACCESSION   DAOPUW010000027 DAOPUW010000000
VERSION     DAOPUW010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14587_length_5557_cov_3.287714"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1711)
                     /gene="metG"
                     /locus_tag="PKJ43_00930"
     CDS             complement(<1..1711)
                     /gene="metG"
                     /locus_tag="PKJ43_00930"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNZ71149.1"
                     /translation="MNNFKRTLITSALPYANGPVHIGHLAGVYVPADIYARYLRMKGE
                     EVAFIGGSDEHGVPITIKAKNEGVTPQDVVDKYHNIIKDSFARFGISFDVYSRTSAKV
                     HHETASEFFKKLYDEGKFIEKTSEQYYDEEAKQFLADRYIIGTCPKCGNEKAYGDQCE
                     ACGSSLNATDLINPKSTLSGNIPVMKETKHWYLPLDQYEPWLREWILEGHKEWKSNVY
                     GQCKSWIDGGLNPRAVTRDLDWGVPVPVDGADGKVLYVWFDAPIGYISATKELTPEWK
                     KWWKDPETRMLHFIGKDNIVFHCIIFPAMLKAEGTYILPENVPANEFLNLEGDKISTS
                     RNWAVWLHEYLEEFPGKEDVLKYVLTANAPETKDNDFTWKDFQARNNNELVAILGNFV
                     NRALVLTQKYYNGVVPAAGELLDVDKDALAQIVSLKADVEKSLDTFRFREALKIAMDM
                     ARLGNKYLADMEPWKVVKTDEERVKTIMNTCLQIAANLTIVFAPFLPYTMNKLRGFLN
                     MEHLNWSELGRTDILVAGHKVNEPELLFEKIEDAVIEAQIKKLQDTKKANEMAAAKAA
                     PQKE"
     gene            1909..2322
                     /locus_tag="PKJ43_00935"
     CDS             1909..2322
                     /locus_tag="PKJ43_00935"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNZ71150.1"
                     /translation="MNRLILLLALLISLMLFSCNSANQSTRVVKGKSQSVYTNEPFVG
                     MVTLTYHKEKLASVDFQIVDTLHNEVFGSDYEKHYPDNEEYRMQCRNDWQGVLSYPAQ
                     LVKKQDIGAIDAISGATWSYNLFSAAVKNALENNN"
     gene            2424..3548
                     /gene="holB"
                     /locus_tag="PKJ43_00940"
     CDS             2424..3548
                     /gene="holB"
                     /locus_tag="PKJ43_00940"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNZ71151.1"
                     /translation="MQFRDVIGQKEIKKRLIDGVNENRISHALLFSGPSGVGKLAMAI
                     AYSQYINCKNRGEEDSCGECPSCKKYAKLIHPDLHFVFPVVKSKKFKDPVSDNYIDKW
                     RAQVLSNPYFNIDSWNAQLDVENSQAQIFVDESTEIIRKLSLKTFEAEYKVMIVWMAE
                     RMNAQCANKLLKMIEEPPPKTLFVLITEEEERLLATIKSRTQLVKFIGIDNESMAKAI
                     EKNPLAQGKNSKGLAHLANGSYITALDLLNPNDQAILYNEKFTGLMRSSYGRAWESLF
                     LLADELAGWGREQQKSFLLYCMKMIRENFIMNFKQNDLVFLNELDRKFSEKFSPFINE
                     RNVYQFTKEFQDAFRDISQNGNSRIIFLDLTLKVVKLIKQ"
     gene            3677..4969
                     /gene="ricT"
                     /locus_tag="PKJ43_00945"
     CDS             3677..4969
                     /gene="ricT"
                     /locus_tag="PKJ43_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNZ71152.1"
                     /translation="MDTGNQIYRGCPSSGRAGTCAKLEVYDWLGDVENILNPEELVEI
                     RFKNTRKDYFRNVNLLKLEVGDLVAVEASPGHDIGMVSLTGDLVRRQMKRHKVTLVNG
                     EMRKVYRKAKPVDIDKWHEAMAREHETMIKSRHIAADLRLNMKIGDVEYQGDGTKAIF
                     YYIAEDRVDFRQLIKVLADSFRIRIEMKQIGARQEAGRIGGIGPCGRELCCCSWMSSF
                     SSVSTGAARYQEISLNPQKLAGQCSKLKCCLNYEVDSYIDEQKSFPPRIRLETQEGVF
                     QYIKSDIFKNVYWYSLDSRVPSGQIGIPVERVHRIIQMNRKGHLPEKLVDANDQPVSA
                     EKPSNDYGNVVGQDSLTRFDKPRDNNRRNRNQNKRRPRPSEGQQGEQVQKNQPQNAQP
                     RDNENQPRRDNQQRRHIKRVNRPGGNPQGRPNNQGRPE"
     gene            4993..5460
                     /locus_tag="PKJ43_00950"
     CDS             4993..5460
                     /locus_tag="PKJ43_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HNZ71153.1"
                     /translation="MKRLFFFFFLISTLFFSCKSNSLYEDYRAIKASGWEQDSVAVFK
                     FDVEKNYLNYSLSINVRNRGDYQFSNLWLFVDITSPGNVTVSDTIEYQLSYPDGRWTG
                     KGTGGIFSNQFLFRDNVFFPIPGSYTVSIRHAMRPNPLVGISDIGICVKRKQP"
BASE COUNT         1575 a         1147 c         1274 g         1561 t
ORIGIN      
        1 tttctttttg cggggcagcc tttgcagcag ccatctcgtt ggcctttttg gtatcttgaa
       61 gctttttgat ttgggcttca atcaccgcat cttctatctt ttcgaaaagc agttcaggtt
      121 cgttcacctt atgacctgca accagaatgt cggtacggcc aagttcactc cagttgaggt
      181 gttccatgtt gaggaagccg cgcagcttgt tcatggtgta gggcaggaat ggcgcaaaca
      241 cgatggttag gttggcggca atctgtaaac aggtgttcat gatggtcttc acgcgctctt
      301 cgtcggtttt caccactttc catggctcca tgtcggccag gtacttgttt ccgaggcgcg
      361 ccatgtccat tgcaattttc agggcttcgc ggaaacggaa ggtgtcgagg cttttctcta
      421 catcggcttt cagcgaaaca atctgggcaa gggcatcctt atcaacatcg agcaattcgc
      481 cagcagcagg caccacgccg ttgtaatatt tttgggtgag caccaatgca cggttcacaa
      541 agttgccaag aatggctaca agttcgttgt tgttgcgtgc ctgaaaatct ttccaggtaa
      601 aatcgttgtc cttggtttcg ggagcatttg cagtaagcac atatttcaat acatcttcct
      661 tgccgggaaa ctcttcaaga tattcgtgca accaaactgc ccagttgcgc gaggtcgaaa
      721 tcttgtcacc ttcgaggttc aggaactcgt ttgccggaac gttttcaggc agaatatagg
      781 ttccttcggc tttcaacatg gccggaaaaa tgatgcagtg gaaaacaata ttgtcttttc
      841 caataaaatg caacatgcgg gtttcggggt ctttccacca ttttttccat tcgggagtca
      901 actctttggt ggccgaaatg taaccaatag gcgcatcgaa ccacacgtaa agcactttgc
      961 catcggcacc atcgaccgga actggtacgc cccaatctag gtcgcgggtc acggcgcgag
     1021 ggttcaaacc gccgtcgatc cagcttttgc actgtccgta cacgttgctt ttccactctt
     1081 tgtggccttc gagaatccac tcgcgtagcc agggttcgta ttgatcgagc ggcaggtacc
     1141 agtgtttggt ttctttcatc accgggatgt ttccgctcag tgtcgatttt gggttgataa
     1201 ggtcggtcgc gttcaacgac gatccacagg cttcgcattg gtctccatac gctttctcgt
     1261 taccgcactt ggggcaggtt cctatgatgt aacggtcggc gaggaattgc ttggcctctt
     1321 cgtcgtaata ctgttcgctt gttttttcta tgaatttgcc ttcgtcgtaa agctttttga
     1381 aaaattcaga agctgtttcg tggtgcactt tggcactggt gcgcgaatac acatcgaacg
     1441 aaattccgaa acgggcaaac gaatccttaa tgatgttgtg gtatttgtcc accacatcct
     1501 gcggggtaac cccttcgttt ttagctttta tggtaatggg cacaccgtgt tcgtcgctgc
     1561 cgccaataaa ggcaacctct tcgcccttca ttcgtaggta acgggcataa atatcggcag
     1621 gcacataaac tccggccagg tggccaatgt gtacaggtcc gttggcataa ggcaacgcgg
     1681 acgtaatgag tgtgcgttta aaattgttca tgataaaatg aattgaattt cagtgctaaa
     1741 aattaaggcg acaaagatag gaaaaatgta taaaacagat gttgatagtt agcctgaacc
     1801 tttttaatcc ctatgaatta ccatcaaaga aaatagaaat gcaaaaatta ctgtacgtta
     1861 ggataagggt gaaaatttat ttgcttgtct aatcttaaaa aaacagccat gaatagatta
     1921 attttattgc tagccttact tatttctttg atgctttttt cctgtaattc tgccaatcag
     1981 tctacccgtg tagttaaggg caaatcacaa tcggtttata ccaacgagcc ttttgttgga
     2041 atggtcaccc tgacttatca taaggaaaaa ttagcatctg ttgatttcca aattgtcgat
     2101 acacttcata atgaagtttt tgggagcgat tacgagaaac attatcccga taatgaggaa
     2161 tacaggatgc aatgtcgaaa tgattggcaa ggggtattat cgtatccggc tcagttggta
     2221 aaaaagcagg atattggtgc gatcgatgct atttcggggg caacctggtc gtataattta
     2281 ttttcggcag ctgtaaagaa tgcattggaa aacaacaatt aaaagggaga ttttgggaga
     2341 aacgctgatt gaggagagcc tattttttga atttcatttt ttgggctctc ccaatctctg
     2401 attttttttc catatttgta cctatgcagt ttcgtgatgt tataggacaa aaagagatta
     2461 aaaagcgcct tatcgatggt gttaatgaaa atcgtatcag ccatgcgttg ttgttttcag
     2521 gaccttcagg tgttggtaaa ctagctatgg caatagctta ttcacaatac attaactgca
     2581 aaaatagagg cgaagaagat tcctgcggtg aatgtccttc gtgcaagaaa tatgcaaaac
     2641 ttattcaccc cgaccttcat tttgtttttc cggttgttaa gtcgaaaaaa tttaaagacc
     2701 cggttagcga caattatatc gacaaatggc gggcacaagt tttaagcaac ccatacttca
     2761 acatcgattc gtggaatgcc cagctcgatg ttgagaactc ccaggctcag atttttgttg
     2821 acgaaagtac cgagatcatc cgaaaactta gcttaaaaac ctttgaggct gagtataagg
     2881 taatgattgt ttggatggca gagcgcatga atgcccaatg tgccaataag ctattgaaaa
     2941 tgattgaaga acctccgcca aagacattgt ttgttcttat aaccgaagaa gaagaaaggc
     3001 ttttggccac cataaaatca cgtactcaac tggtgaaatt tattggcatc gataatgaat
     3061 cgatggcaaa ggcgatcgaa aaaaacccat tggcacaagg aaaaaacagc aaagggttag
     3121 cacatttggc taatggtagt tatatcaccg ctttagacct tctcaatccc aatgatcaag
     3181 ccatcttgta taatgaaaaa tttacaggtt taatgcgttc atcgtatggt cgggcatggg
     3241 agtccctgtt tttattggcc gacgagcttg ccggctgggg gcgtgaacag caaaaaagtt
     3301 ttctgctcta ttgcatgaaa atgattcggg aaaactttat catgaatttc aaacagaacg
     3361 atttggtttt tttgaatgaa ctcgacagaa aattttcaga gaagtttagc ccatttatca
     3421 acgaaaggaa cgtgtaccaa tttacaaaag aatttcagga tgcttttcgt gatatatcgc
     3481 agaacggaaa ttcgcgcatc atctttctcg atctgacttt gaaagtggta aagttgatca
     3541 agcaatagaa accggcaaat actgaaatag ggaataaagc agttggttgg tttcaactat
     3601 cacttttata actatttttg caacattaac aattcaatat atagcctttt gaagtgaaaa
     3661 aggcacaata gaaaatatgg atacaggtaa ccagatatac agaggatgcc cttcgagtgg
     3721 aagggccgga acatgtgcta aattagaagt ttacgattgg ttgggcgatg tcgaaaacat
     3781 acttaacccc gaagaacttg tcgaaatcag gtttaagaat acccgaaaag attatttcag
     3841 aaatgttaat ttgctgaaat tggaagttgg cgatttggtt gctgtagagg ctagcccggg
     3901 tcacgatatt ggtatggttt cacttaccgg cgatttggtt cgccgccaaa tgaaacggca
     3961 caaagtaacc ctcgttaacg gcgaaatgcg aaaagtgtat cgcaaggcca aacccgtcga
     4021 catcgataag tggcacgaag ccatggcccg cgagcacgaa accatgatca agtcgagaca
     4081 tattgctgcc gaccttaggt tgaacatgaa aattggcgac gttgaatatc agggcgatgg
     4141 aaccaaggct attttctact acattgcaga agatcgcgta gattttcgtc agttaatcaa
     4201 ggtcttagcc gactctttca gaatccgcat cgaaatgaaa cagattggtg ctcgtcagga
     4261 agctggccgc atcgggggta ttgggccttg tggccgcgaa ttatgctgtt gctcgtggat
     4321 gtcgagtttt agctctgttt ctactggtgc agctcgttat caggaaattt cgctcaatcc
     4381 gcaaaaactt gccggacaat gtagcaaact caaatgctgt ctcaactacg aggtcgatag
     4441 ctatattgat gaacaaaaat cctttcctcc aagaattaga cttgaaacac aagagggtgt
     4501 atttcaatac attaaatcag acattttcaa aaatgtctat tggtactcgc tcgattcaag
     4561 agtgcctagt ggtcaaattg gtatcccggt tgaacgggta caccgcataa tccagatgaa
     4621 ccgtaaggga catttgcccg aaaagcttgt cgatgcgaac gaccaaccgg tgagtgctga
     4681 aaagccttcg aacgactatg gaaatgttgt tggacaagac agtcttaccc gtttcgataa
     4741 acctcgcgat aacaaccggc gaaaccggaa ccagaataag cgcaggccaa ggccatctga
     4801 aggacaacag ggcgagcagg ttcaaaaaaa ccagcctcag aatgcccagc cccgcgataa
     4861 cgaaaatcag ccaaggcgtg ataatcagca gcgccggcac ataaagaggg ttaacaggcc
     4921 aggaggcaat ccgcaaggca ggccaaataa ccaagggcgg ccagagtaaa aagaacataa
     4981 aaaagcaagc agatgaagcg tctatttttc ttctttttcc tgatttcaac cttgtttttt
     5041 tcttgtaaaa gcaattcgtt gtacgaagat taccgtgcaa ttaaagcttc ggggtgggag
     5101 caggattcgg tagctgtttt taagttcgat gttgaaaaaa actacttgaa ttattctcta
     5161 tctatcaatg tcagaaatcg tggcgattat cagttcagta atctttggct gtttgtagat
     5221 atcacttcgc ccggcaatgt tacggtgagc gataccattg aatatcagtt gtcttatcct
     5281 gatggccgtt ggacaggtaa aggaacaggc ggcattttct caaatcagtt tttatttcgc
     5341 gacaatgtgt tttttcccat tccgggaagc tatacagtgt cgatcaggca tgccatgcgc
     5401 ccaaatccgc tcgtgggaat tagtgacatc gggatttgtg ttaaacgcaa acaaccataa
     5461 cttcaacatc aattcaactt cacgtgggta aaaataagct ttcaaaattt gctgaaatgc
     5521 aatcttttag caatgttctg caagttacat tcagtca
//
LOCUS       DAOPUW010000028         5554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_14594_length_5554_cov_2.792144, whole genome shotgun sequence.
ACCESSION   DAOPUW010000028 DAOPUW010000000
VERSION     DAOPUW010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14594_length_5554_cov_2.792144"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PKJ43_00955"
     CDS             <1..243
                     /locus_tag="PKJ43_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71154.1"
                     /translation="LVIFSAWTVKQKLIGGAALFVILALINSFRIVMLGLIGRLYPQI
                     FNVMHDYVWNILLVIIIWALWELANRKINKLNAALV"
     gene            240..803
                     /locus_tag="PKJ43_00960"
     CDS             240..803
                     /locus_tag="PKJ43_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71155.1"
                     /translation="MKKYLRTVVFIVSSFLFYLLWVNGGEQAYGKLVTVGIDKITSKV
                     SGIKKAEFKHFDEEEKTLIYCYYSDRKTNIALEFCLPIVLLLAWHLSLFFDNRIRPKV
                     AFKLFAFNFLIVYFLQIMFPLLLYNISQSKAKATGLFIGLQVFGFIVFFLMIKDSLLI
                     KLKYSSEIENQEPVQRVVSTIEKNKKK"
     gene            800..1339
                     /locus_tag="PKJ43_00965"
     CDS             800..1339
                     /locus_tag="PKJ43_00965"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNZ71156.1"
                     /translation="MRTTIKLLILFIVVVSPLSLVRAQDKSEAPVIGLNVGNKAPEII
                     EKGMNDQVVKLSDLKGKIVLIDFWASWCGPCRKENPNVVAAYKKYKDTEFKNGKGFTV
                     FSVSLDQDKDAWLKAIIADKLEWPSHVSDLKGWYAKSAGIYKVNSIPSSYLIDGDGII
                     IGKNLRGPLLDQALDQLKK"
     gene            1668..2297
                     /locus_tag="PKJ43_00970"
     CDS             1668..2297
                     /locus_tag="PKJ43_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNZ71157.1"
                     /translation="MKEKSDMIDKDKIVDEKSEEVENVAGSATNSDDNLADDHSASKK
                     ANHSKESKKNKKDKSEEIIEKLNAELERRNDQYLRLQAEFDNYRKRTLKEKMELTKSA
                     GESILVNILPVIDDFERALNAMETATEIAPVKEGIDLIYKRFSDFIRSNGVKEIEAME
                     QELDTDLHEAITKIPAPVDELKGKIVDVIQKGYTLNDKVIRYAKVVIGE"
     gene            2308..3462
                     /gene="dnaJ"
                     /locus_tag="PKJ43_00975"
     CDS             2308..3462
                     /gene="dnaJ"
                     /locus_tag="PKJ43_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNZ71158.1"
                     /translation="MSKRDYYEVLGVSKDASPEEIKKAYRQKAIQYHPDKNPGDKESE
                     EKFKEAAEAYEVLSNSEKKQRYDQFGHAGMGNGSGGFSGQNMNMDDIFSMFGDIFGGH
                     FGGFGGFGGSSRGRQQRVNRGQNLRVKVKLNLQEIAKGTEKKIKVKKYVACNTCNGTG
                     AKDGSYSTCSTCHGSGQVTRISNTFLGQMQTTSACPTCGGEGKIITNKCTTCYGEGIV
                     QGEEVISLNIPAGVAEGMQLSVSGRGNAARRGGINGDLIVLIEEEEHPELIRDEQDLI
                     YNLFLNFPDLALGTTVEIPTVEGKVKVKIDAGTQSEKILRLRGKGLPDVNGYRKGDIL
                     VKVHAWIPTRLTADEKKLLEKLAQSPNFQGSPQTGPNKKDFFEKMKGMFE"
     gene            complement(3549..4361)
                     /locus_tag="PKJ43_00980"
     CDS             complement(3549..4361)
                     /locus_tag="PKJ43_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71159.1"
                     /translation="MKKIFTLFVFSLLVTFTFSQNKNSTGGTNYYDAAGLVGQVLPAL
                     SAPTGNALVFVDESHSNDGVVSSLTNNGYAVTVASDWYDFNLKIANGQFQLAVAFAQN
                     YSAVSNGLSTTGLKNFIDNGGSVMFATWTAFDASVVTIMEAAFTGNNNLSTVTITDAA
                     LANGLTNPFSLSNPGWGIYSMGLSAIGGGEVVAVFENGDAAMVRGNNGHTMMIGYLSD
                     APNNSTEVRESIIANIVTAANVTVPISYWWIILAFVVIAGSVVFTKRKVIFG"
     assembly_gap    4497..4506
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4631..>5554)
                     /locus_tag="PKJ43_00985"
     CDS             complement(4631..>5554)
                     /locus_tag="PKJ43_00985"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HNZ71160.1"
                     /translation="FIFVRNWFCFTLVLLLICNTAICQSLPDSVKPPPVNKKMLTAAI
                     VTESTIYTAGMAYLQFVWYKDHQRVPFEFYDDHKGYLQIDKLGHAFAAYGESYVAYQW
                     LRKAGVSKNKALLYGGTMGFVMQAPIEIFDGLYEGWGFSWSDIIANSAGSALLIGQEL
                     LFDKQIVRYKISFSRSEMARNAYGYLGNNALDGFFYDYNGHSFWLSANANRLFMHDIL
                     PPWINIAVGYSASGMLGEFENRSYYRGHKLPEYDRTRQYLLSLDIDWEKIPTHSRFLK
                     AVFSGLNFVKLPFPTLEVNSQGKFKGYWLYF"
BASE COUNT         1764 a          979 c         1238 g         1563 t
ORIGIN      
        1 ctggttattt tttctgcgtg gactgtaaaa cagaaattaa taggtggcgc agctttattt
       61 gtaatactgg cccttattaa ttcattccgg attgtaatgc ttgggcttat tggccggctc
      121 tatcctcaaa tattcaatgt aatgcacgat tatgtctgga atatattgct ggtgataatc
      181 atttgggcgc tttgggagct tgccaaccga aagattaaca aattaaatgc cgcattagta
      241 tgaaaaagta tctgcgaacc gtggttttta ttgtatccag tttcctgttt tatcttttgt
      301 gggttaatgg tggggaacag gcttatggta agttagttac cgttggaata gataagataa
      361 catctaaagt ttcgggcatt aaaaaggccg agttcaagca tttcgatgag gaagaaaaaa
      421 cgctgatata ttgctattat agtgacagaa aaaccaacat agccctcgaa ttctgtttgc
      481 ccatcgtctt attattggca tggcatttgt cattattctt cgacaaccgc attaggccca
      541 aggtggcatt taagttattt gcttttaatt ttttgattgt atattttttg cagataatgt
      601 ttccattact gctttataat atcagtcagt cgaaagcaaa agctacgggg ctttttatcg
      661 ggttacaggt ttttgggttt attgtgtttt tcctgatgat aaaagattca ctgttgataa
      721 aactcaaata ttcttcagaa attgaaaacc aggaacccgt tcagcgtgtt gttagtacaa
      781 ttgaaaaaaa caaaaagaaa tgaggacaac gattaagtta ttgattttat ttattgtagt
      841 agtatctccg ctttccttgg tgagagccca ggacaagtcc gaagctccgg taattggatt
      901 gaatgttggt aacaaagctc ccgaaattat tgaaaaggga atgaatgatc aagttgtgaa
      961 attgtcggat cttaaaggca aaattgtgtt aatcgacttt tgggcttctt ggtgtggccc
     1021 ttgccgaaaa gagaatccta atgttgtagc tgcttataaa aaatataagg ataccgaatt
     1081 caaaaatggg aaaggattca ctgtatttag tgtttcactc gatcaggaca aagacgcatg
     1141 gttaaaagcc attattgccg ataaactgga atggccttca catgtaagcg acttgaaagg
     1201 ctggtatgcc aaatcggctg gaatttacaa agttaacagt ataccaagca gttatctaat
     1261 cgatggtgat ggcataatca ttgggaaaaa ccttcgtggt ccattattgg atcaagctct
     1321 cgatcagctt aaaaagtaaa ttcatcaggg gtaaacaatt gatcgttaag gggttatttg
     1381 ttatttccta tgttaataat ttatacaaat tgaaaagccg cttgtaaagt ggcttttttt
     1441 gtcatgtttt cagttaacca gtgccattat gtcgggctaa ggccgtttgg caaaatattt
     1501 gtaatttcac cgctcgtttt ttagagctat aaagtcgaat ttctatggtg aaatcaactc
     1561 atttgagcag tggttattaa gaattatagg attctttgaa atcaaaatca attgccacca
     1621 gagtttgaat ttgatgaata tgaaagaaac tgaaaagaaa aaaataaatg aaagagaaaa
     1681 gtgatatgat tgataaagac aaaattgttg acgagaaaag cgaagaggta gagaatgttg
     1741 ctggcagtgc aaccaattct gatgataatt tggcagatga tcattctgcg tcgaagaagg
     1801 caaaccattc gaaagagagc aaaaaaaata agaaagataa atcagaagag attattgaaa
     1861 agttaaatgc cgaactcgaa cgtcgcaatg atcagtatct taggcttcag gcagaatttg
     1921 ataactaccg taaacgtacg cttaaggaaa aaatggaact cacaaagtcg gcaggagaat
     1981 cgattttggt aaacattttg cctgtaatcg atgattttga acgcgctctt aatgctatgg
     2041 aaacggctac cgaaatagcc ccggttaaag aaggcatcga tttgatttac aagaggtttt
     2101 ctgattttat aagaagcaat ggagtgaagg aaattgaagc tatggagcaa gaacttgata
     2161 ccgacctcca tgaagcgata acaaaaatac ctgcacctgt tgatgaacta aaaggtaaga
     2221 tcgttgatgt aatccaaaag gggtatactc tcaacgataa ggtaatacgt tatgcaaaag
     2281 tagttatcgg cgaataatat aaataaaatg agtaaacgcg actattatga agtgctgggg
     2341 gtttcgaaag acgcttcgcc cgaagaaata aagaaagctt accgacaaaa ggcgattcaa
     2401 taccaccccg ataaaaaccc gggtgataaa gaatcggaag aaaaatttaa agaagcagcc
     2461 gaagcttacg aagtacttag caactctgag aaaaagcaac gttacgacca gtttggacat
     2521 gccggaatgg gtaatggatc cggtggtttt agcgggcaaa acatgaatat ggatgacata
     2581 ttctctatgt ttggtgatat ttttggtggc cattttggtg gattcggtgg gtttggcggc
     2641 agtagtcgcg gccgtcagca acgggttaac cgggggcaga accttagggt aaaagttaag
     2701 ctcaacctgc aagaaatcgc caaaggaacc gagaaaaaaa tcaaagtaaa aaaatacgtt
     2761 gcctgtaata cctgtaatgg tacaggggct aaagatggtt catattcaac atgttcgacc
     2821 tgtcacggta gtggtcaggt aacccggata tcgaatacct ttcttggtca gatgcaaacc
     2881 acatcggctt gtccgacttg tggtggcgaa gggaaaataa tcaccaataa atgcacaact
     2941 tgttatggtg aaggcatcgt tcagggcgaa gaggtcataa gccttaatat tcctgctgga
     3001 gttgccgagg ggatgcagct ttcggtgagt ggcagaggaa atgctgcccg tcggggaggt
     3061 ataaatggcg acctgatcgt cttaattgaa gaagaggaac acccagaatt gatcagggac
     3121 gaacaagacc tgatatataa cttgttcctt aatttccctg atttggcgtt gggtacaaca
     3181 gtagaaattc caactgtgga aggaaaagtt aaagtaaaga ttgatgccgg gacacaatct
     3241 gaaaaaattc ttcggcttcg tgggaaaggc ctccccgatg ttaacggcta ccgaaaaggt
     3301 gacatcttgg taaaagtaca tgcttggatt cctacacgtt tgacggccga tgaaaagaag
     3361 ttgcttgaaa aattggctca gtctcccaat tttcagggat caccgcaaac agggcctaac
     3421 aaaaaggatt tcttcgagaa gatgaaaggg atgtttgaat agatcgtggt gatttaattt
     3481 ataccatata taataaaaaa tagggaggta ctaagcagtg cctccctatt tttttaaata
     3541 tcgtacgatt atccaaatat tacttttcgc ttagtgaata caacacttcc ggcaattacg
     3601 acaaatgcca gaataatcca ccaataagag atgggtacgg ttacattggc cgctgttaca
     3661 atattggcga taatgctttc cctaacctct gtggagttat ttggcgcatc agataggtaa
     3721 ccaatcatca tggtgtgtcc attatttccc cgaaccatgg ctgcatctcc attctcaaaa
     3781 acggcaacaa cttcaccacc tccaattgct gataaaccca tggaataaat accccatcca
     3841 ggattagaga gggagaaagg gttggttaac ccgttggcaa gtgccgcatc tgtaatagtg
     3901 acagtgctaa ggttattatt gccggtgaat gcagcttcca ttatggtgac aacagatgca
     3961 tcaaaagcgg tccacgttgc aaacataaca ctgcctccgt tgtcgataaa gtttttaagc
     4021 ccggtagtgc taagtccgtt agaaactgct gagtaatttt gggcaaaagc aactgcaagt
     4081 tggaattggc cattggctat tttaaggtta aagtcatacc agtcactggc aacagtaact
     4141 gcatatccat tgttcgtaag cgaggaaacg actccatcat tggagtggga ttcatcaaca
     4201 aaaacaaggg cattgcctgt tggcgcagat aatgcaggaa gaacttgacc aactaaacca
     4261 gcagcatcat aataattggt tcctcctgtg ctgttcttat tttgtgagaa agtaaaagta
     4321 acaaggagtg agaacacaaa aagggtaaaa attttcttca tatcaataaa gttttaaatt
     4381 gagacagaat tatggtttaa ttaattaaaa caaaagtata tttttttttt tgatctaacc
     4441 aaggatcaat ttaaaaagtt gggggattaa aaaaataaga tgagttttgt gtttttnnnn
     4501 nnnnnnaatt tatgcgtaaa ctataaaatc ccctgaaaaa tgaattgatc cctaaccaat
     4561 tcgaatgtat tcagattttt taatgattga tgggttgtta gccatccctt tttttgtttg
     4621 attagcaaaa ttaaaaatag agccaatatc ctttgaattt tccttgcgaa ttgacctcca
     4681 gtgtagggaa ggggagtttc acaaaattga gccccgagaa aacggccttt aaaaagcggg
     4741 agtgagtagg gatcttctcc cagtcgatgt cgagggatag caggtattgg cgggtgcggt
     4801 cgtattccgg gagcttatgg ccgcgataat agctccggtt ttcgaattcg cctaacatgc
     4861 cacttgcact atatcctaca gcaatattta tccaaggagg caaaatgtcg tgcataaaaa
     4921 gacggttggc attggccgaa agccaaaaac tgtgtccgtt gtagtcgtag aaaaatccat
     4981 caagggcatt attgcccagg taaccgtagg cattcctagc catttcggag cgcgaaaagc
     5041 tgattttgta acgcacaatc tgtttatcga aaagtaattc ctgaccaatg aggagggcac
     5101 ttccggcact gttcgcaata atatcgctcc acgaaaaccc ccagccttca tataaaccat
     5161 cgaatatttc aatgggcgct tgcatcacaa agcccatagt tcccccatag aggagtgcct
     5221 tatttttgct caccccggct tttcttagcc attgatatgc cacatagctt tctccatagg
     5281 cggcaaatgc gtgacctaat ttgtcgatct gcagataacc tttgtggtca tcgtagaact
     5341 cgaaaggaac gcgttggtgg tctttatacc acacaaattg aaggtaggcc atcccggcag
     5401 tatatatggt gctttcagta actattgctg cagtaagcat ttttttgttc acaggaggag
     5461 gctttaccga gtcgggaagt gactggcaga tagctgtatt gcatatcagc aatagcacaa
     5521 gggtaaaaca aaaccaattt ctaacgaata tgaa
//
LOCUS       DAOPUW010000029         5526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_14695_length_5526_cov_2.701700, whole genome shotgun sequence.
ACCESSION   DAOPUW010000029 DAOPUW010000000
VERSION     DAOPUW010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14695_length_5526_cov_2.701700"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PKJ43_00990"
     CDS             complement(<1..704)
                     /locus_tag="PKJ43_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71161.1"
                     /translation="MIRTITLIIIGILLSLNTRSASLLIPMDNAQKNHLKAYGIAFWS
                     LQRAVEVKWLLNYRGGSFMLPQIQEIENECLIRGVNYEVIADGQANLIVEEISSPSVN
                     MDVILMQKAPKIAVYSPPNKQPWDDAVTLVLTYAEIPYDIIYDEEILDGKLSEYDWLH
                     LHHEDFTGQFGKFFRNYQHMPWYQEEVRTNQQLAQKLGFLKVSEMKLVVSKTIKNYVL
                     NGGFLFAMCAATDTYD"
     gene            complement(706..2235)
                     /gene="dnaB"
                     /locus_tag="PKJ43_00995"
     CDS             complement(706..2235)
                     /gene="dnaB"
                     /locus_tag="PKJ43_00995"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010421108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNZ71162.1"
                     /translation="MATTSKRNTKATIEEINAQYGKIPPQAVDVEEAVLGALMLEREA
                     FIGIADLLSEESFYKEEHREIFKVIKDLSMRDQPVDLLIVTQELKNRGLLEKVGGPLY
                     ITQLTSKVASAAHLEFHARIIAQKHIQRELIRSCSEIQVNAYDDTKDVDELINEAESA
                     IFKISEGNIKKETQPIKPILKEAVQLIEEASKRKDGLSGIPSGFSKVDRITSGWQKTD
                     LIIIAARPAMGKTAFVLSMARNMAVEFNKPVAIFSLEMSSIQLVNRMIASESELGSDK
                     IKKGNLEGDDWDRLNKAIRRLDEAPVFIDDTPALSIFELRAKCRRLKMQQDIGIVLVD
                     YLQLMTAGGDNRGSREQEVSMISRSLKAIAKELDIPIIALSQLNRSVESREGKRPQLS
                     DLRESGAIEQDADMVIFIHRPEYYGITEDDEGNSLRNVAEIIIAKHRNGAVGDVRLSF
                     RKELARFVDLEEYPSDFDPMAMQTFSSKMNEDRGGNFNPPAFSIPVGNPFESSTDDIP
                     F"
     assembly_gap    2266..2365
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2477..3463)
                     /locus_tag="PKJ43_01000"
     CDS             complement(2477..3463)
                     /locus_tag="PKJ43_01000"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002527.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNZ71163.1"
                     /translation="MKKNIAVIWGGDSSEYVVSVKSGRNIFNHIDTAKFTPWLIRMRH
                     DEWHVMNGEELVSPINKSDFSFEINGEKIKIDYAFIMIHGTPGEDGVLQGYFDLLKIP
                     YSATGVYASALTFHKFYCDNFLRAFGIKMAKAEYFKRGDKIDTKQLSNKFGFPLFVKP
                     SAGGSSFGVTKVKSEEQILPAIEAAFAESNDCLVEEFVDGMELAVGVCDLNGKITPFL
                     PTEVIPGGDFFDYNSKYNIGGATEITPARIPAEKIKECQDLTVKLYKLIGCHGIVRID
                     YILKNNEFYFLEVNTIPGMTETSFIPQQLNAMGLNLGDIVSQVIEYDLQKQA"
     gene            complement(3565..>5526)
                     /locus_tag="PKJ43_01005"
     CDS             complement(3565..>5526)
                     /locus_tag="PKJ43_01005"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNZ71164.1"
                     /translation="TETLEEAWLTVGTELKNISRKPITGNLKISTEGMSLVYPVSLSA
                     GEKRTITLSPETQEALHLKNPKLWWCNGLGNANLYTMNVEFIQNGTRKASEKVTFGIR
                     EIETYFTEKGHRGFKLNGKEILIKGAGWTDDIFLRNTNESNEAQVKYVKDMGMNTIRF
                     ENIWGTSQNIYDLCDRYGILAMVGWSCQWEWENYLGKPCDEFGGIETEAEIDLIIRYM
                     RDQILWLRNHPSIFVWLTGSDMLPRPSLEKKYITLFEEIDNRPWLGAASNRKSELSGP
                     TGMKMNGPYEYVFPSYWYLDTLNGGAYGFNTETGPGCQIPVKESVNRFISPENKWPLS
                     SAWDAHCTTSDAAMNSMSLNNEVVSALWGKPKNFDDFLLKSHMVNYEAIRAMFEAFRV
                     NRPNTTGIIQWMLNSAWPSLYWQLIDFYLVPTPAYYGVKKALEPIQLIYNYGDSSIVL
                     SNETLVPAKGLKANITLYDLNSTPQFSKSVMVDAEPGKPIVIGKLPANKSTSLLKLEL
                     FNAKGILIADNVYWLSNKKEIFDWENSNWYTTPMKEYPDYTHFNLLKNIDLPTEYTTR
                     KEAEETIISLNIRNTSETIALFVELKLLDENNEWIVPSLFSDNYVTLIAADQKKVDIS
                     VPTSITSERKAKITVSGWNVNPQIIEIKQ"
BASE COUNT         1354 a         1132 c         1156 g         1784 t
ORIGIN      
        1 atatcgtatg tgtcggtagc ggcacacatg gcaaatagga agcctccgtt aagaacgtaa
       61 tttttgattg tttttgaaac aaccagtttc atttctgaaa ctttcagaaa gccaagtttt
      121 tgtgctaatt gttgattggt acggacttct tcctgatacc agggcatgtg ttggtagttg
      181 cggaaaaact ttccaaattg tccggtaaaa tcttcgtggt gaaggtgtaa ccaatcatat
      241 tcgcttagtt ttccgtcgag aatttcttca tcgtaaataa tgtcgtatgg aatttcggca
      301 tacgtgagca ccagcgttac ggcatcgtcc cagggctgtt tgttaggcgg tgaatacacg
      361 gcaatttttg gtgctttttg catgagtatt acatccatat taaccgaagg gcttgatatt
      421 tcttcgacta tcaggtttgc ctgcccatcg gcaattactt cataatttac accacgtata
      481 aggcattcgt tttcaatctc ctgtatttgt ggcaacataa aacttccgcc gcggtagttc
      541 agcagccatt tcacttcgac agcgcgttgt aacgaccaga aggcaattcc gtatgctttg
      601 aggtgatttt tctgggcgtt atccattggg atgagcaagg atgcgcttcg cgtgttcagg
      661 cttagtaata tgcctatgat tatcagggta attgttctta tcattctaaa atggtatatc
      721 atctgttgaa ctctcaaacg ggtttccaac gggtatcgaa aaagcaggag ggttgaaatt
      781 acctcctctg tcttcgttca tctttgagct gaaagtctgc atcgccattg ggtcgaagtc
      841 ggatgggtat tcttcaaggt cgacaaaccg ggccaattct ttacggaatg aaagccttac
      901 gtcgccaaca gccccgttac ggtgtttagc tataataatt tctgcaacat tccgcaggga
      961 gtttccttca tcgtcttcgg taattccata atattcaggg cggtgaataa aaattaccat
     1021 gtcggcatct tgctcaatgg cacctgactc acgaaggtct gaaagctgag gtcttttccc
     1081 ttcacgcgat tcaactgaac ggtttaactg tgataatgcg attatcggta tatcaagttc
     1141 ttttgcaatt gctttaagtg aacgggagat catactaact tcctgttcac ggctaccccg
     1201 gttatctcct cctgcggtca tcagctggag gtagtcgact aacactatgc caatatcctg
     1261 ttgcattttc aaacggcggc atttggccct gagttcaaag atggaaaggg caggagtatc
     1321 gtcgataaaa actggggctt catcgagcct gcgtatggct ttgttgagcc ggtcccaatc
     1381 gtcgccttcg aggttccctt tcttaatttt gtcagaacca agttccgatt ctgaagcaat
     1441 cattcggttt accaactgga tggaagacat ttcgagtgag aatatggcta ccggtttgtt
     1501 aaattccacg gccatgtttc ttgccattga cagcacgaaa gctgttttac ccattgcagg
     1561 tcgtgcagcg ataattatta agtcagtttt ttgccagcct gaggtaatgc ggtcaacttt
     1621 cgagaaccca gatggaatgc cagacaaacc atcttttcgt ttgctggctt cttcaattag
     1681 ctgtacggct tctttcagga tgggcttaat tggttgggtt tcttttttga tgttaccttc
     1741 cgatatcttg aaaatggccg attctgcttc gttgatcagt tcgtctacgt ctttggtgtc
     1801 gtcgtatgca ttgacttgta tttcggaaca cgaacggatt aactcacgct gaatatgctt
     1861 ttgggcaata attcttgcat gaaattcgag gtgtgctgcc gaagctactt tgctggttag
     1921 ttgggttata tataatgggc ctcctacttt ttcgagcagt ccgcggtttt taagttcttg
     1981 ggtaacaata agcaggtcga ctggctgatc tctcatcgaa aggtctttta tcaccttgaa
     2041 tatttcgcga tgttcttctt tataaaaact ttcttcgcta agtaaatctg ctattccaat
     2101 gaatgcttcg cgttcgagca tcaatgctcc aagcactgct tcttcaacat cgactgcctg
     2161 gggtggaatt ttcccgtatt gggcattgat ttcttcgatt gtagcctttg tatttcgttt
     2221 tgatgtggta gccatacaat taaaatttat ccctccttat ttaggnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnntattt cgttttgatg tggtagccat acaattaaaa
     2401 tttatccctc cttatttagg tggacatcaa atttatgaat tttacccttt aagcgagggc
     2461 tttgttgaat attaatttat gcctgttttt gcaaatcgta ttcaattacc tgacttacaa
     2521 tgtcgccaag gtttaatccc atagcgttta attgttgtgg tatgaagctg gtttcggtca
     2581 tgcctggtat ggtgtttacc tccagaaaat agaactcgtt gtttttcaat atataatcga
     2641 ttctgacgat tccgtggcac ccgataagtt tgtaaagctt aacggtcaaa tcctggcatt
     2701 ctttaatttt ttctgcagga atccttgccg gtgtaatttc tgtggctccc ccaatattat
     2761 atttggaatt gtaatcgaaa aagtctcctc caggtattac ttcggtagga aggaacggtg
     2821 ttattttacc attaaggtca caaacaccaa cggcaagttc catcccatca acaaattctt
     2881 caacaaggca atcgttgctt tcggcaaatg cagcttcaat ggctggcaaa atttgttctt
     2941 cggattttac tttcgtaaca ccaaagctcg accccccagc actgggtttc acaaagaggg
     3001 ggaagccaaa tttattggac aattgtttgg tatctatttt gtcgccgcgt ttgaaatatt
     3061 ccgctttggc catttttata ccaaaagccc ggagaaaatt gtcgcaataa aacttgtgaa
     3121 atgtaagagc cgatgcgtaa acacctgtgg ctgagtaagg tattttaagt aaatcgaaat
     3181 atccttgcag aactccatct tcgccaggtg taccatgaat catgataaag gcatagtcga
     3241 tcttgatttt ttccccattg atttcgaaac tgaaatcgct cttgttaatg ggagaaacga
     3301 gttcttcacc gttcattacg tgccactcat cgtgcctcat acgaatcagc caaggggtaa
     3361 atttggcagt gtcgatatgg ttgaatatgt tacgtccgct ttttacactt actacgtatt
     3421 ccgaagaatc gccaccccaa attacagcta tatttttttt cattgtgttt actttttatg
     3481 gcccaaaaat agaaaagtta ttgccttata ccgatgaaaa tatcaaaata ttcaaaccat
     3541 ggatgatgta ttctaccgta ttcactattg cttaatttcg ataatctgcg gatttacatt
     3601 ccatcccgaa accgtaattt ttgctttccg ttctgaagtg atggatgtag gaactgaaat
     3661 gtccaccttt ttctgatcgg ctgcaatcag ggtaacatag ttgtcggaaa acagagacgg
     3721 aacaatccat tcgttgtttt catccaagag tttaagttcg acaaaaaggg caatcgtttc
     3781 cgacgtattc cgaatattta acgagataat cgtttcttcg gcttctttac gggtagtata
     3841 ttccgtaggt aagtcgatgt ttttaagcaa attgaaatgc gtgtaatcgg gatattcttt
     3901 cattggggtt gtataccaat tactgttttc ccagtcaaat atttcttttt tgttgctcag
     3961 ccagtaaaca ttgtcagcta ttaatatgcc ttttgcattg aaaagctcaa gttttaacag
     4021 cgaagtagat ttgtttgccg gtaacttgcc aataactatt ggtttgccag gttcggcatc
     4081 aaccataact gatttggaaa attgtggggt agagtttaaa tcgtacaacg taatatttgc
     4141 ctttaaccct tttgccggga caagggtttc gttcgatagt acaattgaac tgtctccata
     4201 attataaatc aattgaatag gttccagtgc ctttttaacc ccataatatg cgggtgtggg
     4261 aaccagataa aaatcaatta gttgccagta aagcgaaggc catgcagagt ttaacatcca
     4321 ctgaattatt ccggttgtat tggggcggtt taccctaaat gcttcaaaca tggcccggat
     4381 agcctcatag tttaccatgt gactttttag caagaaatcg tcgaagtttt ttggtttacc
     4441 ccagagggca ctaacaacct cgttgttaag gctcatactg ttcatcgccg catctgacgt
     4501 tgtgcaatgg gcatcccagg ctgatgataa aggccatttg ttttcaggcg aaataaacct
     4561 gtttaccgat tctttgactg gaatctggca tccggggccg gtttcggtat taaacccgta
     4621 tgctccgccg ttcagggtgt cgaggtacca atacgagggg aagacgtatt cgtaaggccc
     4681 gttcattttc attccggtgg ggccagagag ctcgcttttc cggttcgatg cggcacctaa
     4741 ccaggggcgg ttatcgattt cttcaaaaag agtgatgtat tttttttcga gtgagggacg
     4801 gggtaacatg tcgcttccgg taagccaaac gaaaattgaa gggtggttgc gaagccaaag
     4861 tatttggtcg cgcatatatc tgattattaa atcaatttct gcttcagttt caattcctcc
     4921 aaattcatca caaggtttgc ccagataatt ttcccattcc cattgacaac tccagcctac
     4981 catggccaga attccgtaac ggtcgcataa gtcgtaaatg ttttgcgaag tcccccaaat
     5041 gttctcgaag cgaatggtgt tcattcccat gtctttaaca tacttcactt gtgcttcgtt
     5101 gctttcgttc gtgttgcgaa gaaagatatc atcggtccag ccggcaccct taatcagaat
     5161 ttcttttccg ttcagcttaa agcctcgatg tcctttttca gtaaagtagg tttcaatttc
     5221 ccgtattcca aaggttactt tctcgcttgc ttttctcgtg ccgttttgaa tgaattcaac
     5281 gttcattgta taaaggttgg catttcctaa cccattgcac caccatagtt taggattttt
     5341 caggtggagg gcttcctggg tttctgggct tagggttatt gttcgtttct cgcctgccga
     5401 caaagaaacc gggtaaacca gtgacattcc ttcggtagat atctttaagt tacctgttat
     5461 tggttttcgg ctgatgtttt ttaattcagt tcctacggtg agccacgctt cttcgagcgt
     5521 ttcggt
//
LOCUS       DAOPUW010000030         5521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_14722_length_5521_cov_3.401025, whole genome shotgun sequence.
ACCESSION   DAOPUW010000030 DAOPUW010000000
VERSION     DAOPUW010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14722_length_5521_cov_3.401025"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PKJ43_01010"
     CDS             complement(<1..443)
                     /locus_tag="PKJ43_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71165.1"
                     /translation="MYKKIVLVCLIVLLSFAGFSQQAYRIKADIFTKTRLIDSTFQIS
                     KGTLFYDKNVKKIIFDFSFPQKEKVVLFDTIMYSFRADTLYAKSLNLLIPDQSLFHFI
                     LMGNMANFGLDQAHFEMKGVEKKGDMVITTWLPPEFLRQTISKVL"
     gene            complement(545..1015)
                     /locus_tag="PKJ43_01015"
     CDS             complement(545..1015)
                     /locus_tag="PKJ43_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71166.1"
                     /translation="MKTVRSISILFLLITITMVSNATTTEEYSPNFLAEENEIYSAFS
                     EIDPVISYMEEYSNATAAEVNSIFPLASINYNDNFEFFPEEEADRFYFNNQSAFLMGC
                     LLNMWGVLIVAIVNNGDSATVNKSIWGCVASGCVSTGAVVFFYAVIFSSSFYYY"
     gene            complement(1384..1854)
                     /locus_tag="PKJ43_01020"
     CDS             complement(1384..1854)
                     /locus_tag="PKJ43_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71167.1"
                     /translation="MKKSVLKIAAIFMALLTIHLQTFATSAGTTSSENDVVVNYDETE
                     IYASFEEMNDLANYLSENDATYADVENYNSALVENVSSSAAIALSNGSGEPPFISAFW
                     WGCIFNLFGLIVVYVTTDSDSTYTKAAWKGCILSGVLYVAWAIFWNVVGYAAWF"
     gene            complement(1901..2371)
                     /locus_tag="PKJ43_01025"
     CDS             complement(1901..2371)
                     /locus_tag="PKJ43_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71168.1"
                     /translation="MKKHLLKLSSLLMAVALLNIQLMAKPIALAAISENEINSIANFD
                     ESEIYAAFSEIETLETLVKNSEYSYNEVREMNNELISNISVSSAIALSSSENTPPIFG
                     AFLWGCLFNWVGMLIVGLTTDFDNQQITKSFWGCLISTLLFGGGTGCSFYYGYL"
     gene            complement(2726..3727)
                     /gene="trpS"
                     /locus_tag="PKJ43_01030"
     CDS             complement(2726..3727)
                     /gene="trpS"
                     /locus_tag="PKJ43_01030"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNZ71169.1"
                     /translation="MEKPIVVSGIRPTGYLHLGNYFGAVRNFIRMQNDYTCYFFIADI
                     HSLTTHPTPTNLQAGVKQVLAEYLACGIDPEKSTIFIQSDVPEVTELYTLLNMNAYLG
                     ELERTTSFKDKARKNPDNVNAGLLTYPVLMAADIIIHKAQFVPVGKDQEQNLEMARKF
                     AARFNRMYNNELFPIPRPFTFDGKDMIKVPGLDGSGKMGKSEGNAINLCEDPKSIRKK
                     VMRAVTDLGPTEPNSKKAEPVENLFTLMKIVSAPETVAHFESLYNSCEIRYGDMKKQL
                     AEDIISYTDPIRNKINDILADTDYLTKVARMGAERARESAAKTLSEVKSLIGIKSLF"
     gene            3980..4423
                     /locus_tag="PKJ43_01035"
     CDS             3980..4423
                     /locus_tag="PKJ43_01035"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNZ71170.1"
                     /translation="MKKVFFLFLLVFAIAVSCKTKKAASGYVPPVKADVEQTEVVADD
                     AVVSDLPITVKTEEVSVVDTEDQSHDGFAFYVIAGSFSKIENAAKQKMQLIDKGFTPV
                     LLKGENGFIRVAVGHSNIESEARRNVLSIRANYPEHNDVWLLKKK"
     gene            complement(4511..>5521)
                     /locus_tag="PKJ43_01040"
     CDS             complement(4511..>5521)
                     /locus_tag="PKJ43_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ71171.1"
                     /translation="QVSRNFLANDLIISLGLRMDGNSYSAEMQNPLNQFSPRISASYN
                     LSENFSINANAGRFYQMPYYTSLGFKRNDEFVNKANNLKYIRTDHLIAGIEYKPSQVI
                     QFTLEGFYKDYANYPFSVNDQISLANKGNDFGVVGDEEVVSTGTGRALGIELLARINN
                     QKNLNGNISYTLVRSEFEDTKTGKYIPSAWDSRHLLTIFGTYKLKKNWIVGGKWRFVG
                     GLPYTPYDMEKSSSVVAWNALGGPYSDFTRINSERFAAFHQLDVRIDKVFYLNKFTAK
                     FYIDIQNLYNFQAEQNDIVLREKDNLGNFILLDNGTKYSLRRVKNTTGTVLPTLGITF
                     EF"
BASE COUNT         1632 a          993 c         1108 g         1788 t
ORIGIN      
        1 ataagtactt tcgaaatagt ctgtcgtaaa aactcgggtg ggagccaggt tgtgattacc
       61 atatcgccct ttttttcaac tcctttcatc tcgaaatgag cctgatcaag tccgaaattg
      121 gccatattgc ccatcaatat aaaatggaac aacgattggt cgggaatcag caggttcaat
      181 gattttgcat acagtgtatc agctctgaaa ctgtacatta tggtatcgaa aagaacaact
      241 ttttcttttt gtgggaatga aaagtcgaag atgatctttt taacattttt atcatagaac
      301 agagttcctt tgctaatctg gaaggtgctg tcgatcagcc gtgttttggt aaaaatatcg
      361 gcttttatac gataggcttg ctgactgaag cctgcaaatg ataagagcac aatcaggcaa
      421 acaagaacta tttttttgta cataagttga aaatcggtta cgataataga attagttgtt
      481 cctaaaaata gcaactactg caaattaaca cattttaatt gaaaaaaaag tacttgaatt
      541 gttgtcaata gtaataaaag gaagacgaaa atataacggc ataaaagaaa actactgcgc
      601 cggtagaaac gcaaccagag gctacacatc cccatataga cttgtttacg gtagcactgt
      661 cgccgttatt tacaatagca acaataagta cgccccacat atttaacagg cagcccatta
      721 gaaaagctga ttggttgtta aagtagaacc tgtctgcttc ttcttcgggg aaaaattcga
      781 aattgtcgtt atagttgatc gatgcgaggg gaaatattga atttacctca gcggcagttg
      841 cgttactgta ttcttccatg tacgaaataa ccgggtctat ttcactgaaa gcagaataga
      901 tttcattttc ttcagccaga aaatttggac tgtattcttc agttgttgtt gcattcgaaa
      961 ccattgtaat ggtaattagc aaaaaaagaa tggagattga tcttacggtt ttcatttgtg
     1021 tcgaaatttt ggtttttaac tagaattatt tcaacggata attttgtaaa caaaagctag
     1081 gcaacatata ttgcgccacc caaaatattg aatacgatac atccgttggc tgtttgccgc
     1141 acctgatcgc ggttattgtt agtacaagaa gctacaagaa ttataccaac tggatagtaa
     1201 cagcaacctc aaagaaatat cccaaaaaca ggaggctgca attattcttg attattaatt
     1261 acaatagcag gattgggttt ataattgtct ggctggttgc aggatgttga aaatatggtt
     1321 tagatggtta gtttttaaat gagaaaaagg aagttacagt aaagcaactt ccttttttaa
     1381 tttttagaac caggcggcgt aacctactac attccagaat atagcccatg ctacataaag
     1441 aactccgctg aggatacatc ctttccaggc agctttggta taggtgctgt cgctgtcggt
     1501 ggtaacgtaa acaacaatta agccgaataa gttgaatatg caaccccacc agaatgcact
     1561 gatgaatgga ggttcgcctg aaccgttgct taatgctata gctgctgaag aacttacgtt
     1621 ttcaacaagc gcgctgttgt agttttcaac gtcggcatac gtagcgtcgt tttccgacaa
     1681 gtagtttgcc agatcgttca tttcttcaaa cgaagcatag atttctgttt catcgtagtt
     1741 tacaacaaca tcgttttctg aagaagttgt tccggccgat gtagcaaagg tttgaagatg
     1801 aattgtgagc aatgccataa aaatggcggc aattttaagt actgattttt tcatgcgtca
     1861 aaagttaaga tttaacatat ataaattccc taatctttat ttatagatag ccatagtaaa
     1921 atgagcaacc agtccctcca ccaaagagta gcgtactaat caaacacccc cagaaagatt
     1981 tggttatctg ttgattgtcg aaatcggtag ttagcccaac aattagcatt ccaacccaat
     2041 tgaaaagaca accccaaagg aatgcaccaa aaatgggtgg ggtattttct gaactggaca
     2101 gggctattgc agacgataca ctgatgttgc tgataagctc gttgttcatc tctctcacct
     2161 cgttatatga gtattcagag ttttttacaa gagtttcaag ggtttcaatt tcggaaaagg
     2221 ctgcatatat ttcgctttcg tcgaaattgg cgattgaatt gatttcgttt tctgaaatag
     2281 cagccaacgc tataggtttt gccattaatt gtatgttcag taaggcaacg gccattagta
     2341 ggctggagag tttcaaaagg tgttttttca caataaaaaa gtttagttgg tcaattttgt
     2401 tctttatttt taatggatct gttcatattt tttttcgatt aacatgagac agaacttccc
     2461 aattctctga taaaaatatg ttaatcggtt cgaatttcaa acagcctgct gaaaaaacag
     2521 ctgctattta tattcattac tatttacatt gccctttagt attgttgttt ttcttacgtt
     2581 ggattttcat ttccaattat gcatgtttta ttgtgcattg aaattcagaa taatatcaaa
     2641 tgaagcatta cgtttaatct tttcgatttt ttggatagaa taaataagcc ccatttaagg
     2701 agcttcatct atcatatagt tttttttaaa aaaggctttt aataccaatg agcgatttta
     2761 cttcgctaag ggtttttgct gccgattctc ttgcgcgttc agcacccatt cgggcaactt
     2821 tggtcaggta atcggtgtcg gcaaggatat cattaatctt gttcctgatc ggatcggtat
     2881 aactaataat atcctctgca agttgcttct tcatgtcacc ataccgtatt tcgcaagagt
     2941 tgtagaggct ctcgaagtga gcaactgttt cgggcgctga tacaattttc atcagggtga
     3001 aaagattttc tacaggttca gcttttttac tgttaggttc ggtggggcca agatcggtta
     3061 ccgcgcgcat aacttttttc cggattgatt ttggatcttc acagaggtta atggcatttc
     3121 cttccgattt tcccattttc ccgcttccat caagacctgg aacttttatc atgtcttttc
     3181 cgtcaaatgt aaatgggcgg ggaattggga ataactcatt gttatacatt ctgttgaaac
     3241 gggcagcaaa cttgcgtgcc atttcaagat tttgttcctg atcttttcca acaggaacaa
     3301 actgggcttt atgtattata atatcggcgg ccatgagtac cggataagta agcagtccgg
     3361 cattgacgtt gtcggggttt ttgcgggctt tatctttaaa agaagttgtc ctttcaagtt
     3421 cgcccaaata ggcgttcatg ttcaataatg tgtaaagttc ggttacttcg ggcacatcgc
     3481 tttgtatgaa aatggtcgat ttttcgggat caataccaca agccaaatat tctgcaagaa
     3541 cttgtttaac accagcttgc aagtttgtcg gagtggggtg ggtagtgagt gaatggatat
     3601 cggctatgaa aaagtaacag gtataatcat tctgcatgcg gatgaaattg cgaaccgcac
     3661 caaaatagtt acccaaatgc aaatatccag ttggtcttat gccactaaca acgattggtt
     3721 tttccattat ttttcttttt tctgacaagc cacaaaaata taaaaaatca ctttgttgtg
     3781 acccacatcc attctttatc tgtatcttat cttttttcca atgtattcaa tatttttttt
     3841 gatacaattt atgtgaactt ttctcgttaa aatattaata gaaagtgtta ccttacaact
     3901 gtttacttca attttctact tcaaacttat ggtttgggat gttaaatggg ttagaaatga
     3961 ataattaaga aagatcatta tgaagaaagt atttttttta ttccttcttg tttttgccat
     4021 tgctgtttcg tgtaaaacga agaaggctgc cagcggatat gttcctcctg taaaggccga
     4081 tgttgaacaa acggaggttg ttgctgatga tgcagtagtt tccgatcttc ccattactgt
     4141 gaaaacagaa gaagtttcgg tagtcgacac tgaagaccaa tcgcacgatg gttttgcatt
     4201 ttacgttatt gccgggagct tttcaaaaat tgaaaatgcc gcaaaacaaa aaatgcaact
     4261 cattgataaa ggttttacac cagtgttgtt gaagggagaa aatggcttta tcagagttgc
     4321 agttgggcac tcgaatattg aaagcgaggc cagaagaaac gtcctgtcaa ttagggcaaa
     4381 ctatcctgaa cacaacgatg tttggttgtt gaagaaaaaa taagaaaacc atcattaaaa
     4441 aacccggtga attgtgaatc catcgggttt tttgtttctt attcagaagg tataagtttg
     4501 cgaacgaggg ttaaaattca aaggttatcc ctaatgttgg caatactgta cctgttgtgt
     4561 ttttcactct tcgcaacgaa tattttgtgc cattgtcgag gagtataaaa tttccaaggt
     4621 tgtctttttc tctgagtaca atgtcgtttt gttctgcctg aaagttatat aagttttgaa
     4681 tgtcgatata aaatttggca gtgaacttat tgagatagaa aactttatct attcttacat
     4741 cgagttggtg aaatgcggca aaacgttcag aattgatacg agtaaagtcg gagtagggac
     4801 caccaagagc attccatgcc acaaccgacg acgatttctc catatcataa ggagtatagg
     4861 gcaaccctcc aacgaaacgc cattttccac caacaatcca gtttttcttt aacttatagg
     4921 tgccaaaaat ggtgagtaaa tgccggctat cccatgctga aggaatatat ttgcccgttt
     4981 tggtatcttc aaattcactg cgtaccagtg tataagagat gttcccgttt aagttttttt
     5041 ggttgttgat acgggcaagc aattcaatac ccaaagccct tcctgtgcct gttgatacaa
     5101 cttcttcgtc gccgacaaca ccaaagtcgt ttcctttgtt ggctaaacta atctggtcgt
     5161 tcaccgagaa cggataattg gcataatctt tataaaagcc ttcgagggta aactgaatta
     5221 cttgtgaagg tttgtattcg attccggcga ttaaatgatc ggtacgtatg tactttaggt
     5281 tatttgcctt atttacaaat tcatcatttc gtttgaatcc cagcgaagta taatagggca
     5341 tttgataaaa ccgtccggca ttggcattga tactgaagtt ttcgctcagg ttatacgatg
     5401 cagatattcg cggagaaaac tggtttagtg ggttttgcat ttcggctgag tagctgttcc
     5461 catccattct gaggccaaga gatataatca aatcgttggc aagaaaattg cggcttactt
     5521 g
//
LOCUS       DAOPUW010000031         5484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_14859_length_5484_cov_3.836250, whole genome shotgun sequence.
ACCESSION   DAOPUW010000031 DAOPUW010000000
VERSION     DAOPUW010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14859_length_5484_cov_3.836250"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PKJ43_01045"
     CDS             <1..232
                     /locus_tag="PKJ43_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aerotolerance regulator BatA"
                     /protein_id="HNZ71172.1"
                     /translation="ESLQSIADITGGKYFRATSNDKLKMIYEEIDALEKSKIEVKEFS
                     KKQEEFQKYALTAMLLLVAGILLKMSVFRNIP"
     gene            364..1410
                     /locus_tag="PKJ43_01050"
     CDS             364..1410
                     /locus_tag="PKJ43_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010423183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNZ71173.1"
                     /translation="MFRFGSPGYLYALLLIPALVALFIYFRIQRNKALREFGNPEILA
                     PLMPGASGSRPIWKFAIVMLALAFLITGLARPQFGAKLQKINRKGVELIIALDVSNSM
                     LAEDIQPNRLEKAKMAISRLTERLSNDKIGLVVFAGKAYTQIPITTDYASTKMFLNAI
                     NTEVVPVQGTAIGEAINLAMSSFTPSEETSKAIIVITDGENHEDDAIEMAKQAAEAKI
                     TVHTIGMGLPQGGPIPIYNAYGQKDFKRDKSGQVVVTRLDENMLEQIAAAGNGKYIRA
                     NNTEVGINNIFDEINKMEKTELESRIYSDYNDQFFYFIAVAFALLLIEFFVLERKNNL
                     FKNILQLIRGKEEK"
     gene            1493..2344
                     /locus_tag="PKJ43_01055"
     CDS             1493..2344
                     /locus_tag="PKJ43_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ71174.1"
                     /translation="MKRNYLHTIETKNKPSIKIGNWGFTTIVLLLLVSSMSVYGQKER
                     KFIRQGNKHYEKAVANSDSTHIDSVGFSNAEIAYRKALAKKPGDYKSQFNIGNALFKQ
                     NKMAEAASQYQQVIEGGNNKEEKGKNWFNYGNSMLAQQKFDESIDAYKNALRQNPNDV
                     EAKYNLEFARRMKQQQQQQQQQQQQQDKDQNKDQNKDQNKNQNKDQNKDQNKDQNKDQ
                     NKDQNKDQNQNKDQQQQQQQQQSKISKQNAEQLLKALQNDESKTQEKVKKAEAQKAQT
                     RRPDQDW"
     gene            2453..4282
                     /locus_tag="PKJ43_01060"
     CDS             2453..4282
                     /locus_tag="PKJ43_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNZ71175.1"
                     /translation="MNRKIVFIIMLFLGLTSMAEEVRFTMSGPDMVSVGEQFSLTLSL
                     NAEGEDLRMPDLPNFDILMGPSISQSRSYSVVNGKMSQSVNFSYTYILRAQSEGTFTI
                     PPASVKSKRETYQSNQVTIKVIKGRQQATTQSQQGNSAAGQTNSGQAKTGTVSRDNLF
                     MQYEVDKRNVYKGEMILVALKLYSRVSLSVADQTIPSFEGFWAEDIEIPSAEQTQTRE
                     AVDGIIYNVYTLQKKILVPQQTGTLYIEPAEMVFNVQQRVSPQSLFDDFFGSYQNVRV
                     PVKTNRISINVKELPSAPAGFKGAVGKFSLSSSIDKTNIKSNEAITIKTKISGTGNLK
                     HIAAPSFSFPPDFEVYDPKTSYDYNASQSGINGSTTFEQVFIPRYAGKFTIPAENFVY
                     FDPSSQSYKTLSTSEFNINVEKGSDDQSTTVVSSISKQDVKYIGKDIRYIKTENPSLK
                     TRNRLFFGTFNYYFSVIAVLLAFAFFFFFQKKRIAENSNLALVRNRQASKLARKHLNA
                     ASLCLKNNQRDEFFEALSRALWGYLSDKLTLPLSELNRDNAQATLSKYSVEENTISEF
                     IRIIDICEMARFAPTAVNESISDLYGEAETLISNFEKQIRKKA"
     gene            4285..5037
                     /locus_tag="PKJ43_01065"
     CDS             4285..5037
                     /locus_tag="PKJ43_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ71176.1"
                     /translation="MKRIFITILLISGFLGANAQPTNLFKEANNYYAANEFDKAVKSY
                     ESIINQGYESAQLYFNLGNACYKNGELTKAILYYERAKLLSPNDEDIQFNIDLVNQFV
                     VDKIEALPQPFFIKWGQGIVNLFSSNGWAWFSIIVLIMALSGLGIYLFSKNPLFRQIS
                     STTAVVLFSLCILSMVFAGKQKAKLTKHNAAIVFSSTVTVKSSPDNSGTDLFVIHEGL
                     KVQIKDEVGSWYNIRLADGNSGWVQKTVLEKI"
     gene            5053..>5484
                     /locus_tag="PKJ43_01070"
     CDS             5053..>5484
                     /locus_tag="PKJ43_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNZ71177.1"
                     /translation="MKTALIAGATGLVGGELLKQLSEDKRYEKIIVLSRRKLPVQYPK
                     TEVIVSSFDTLESLSLPCPIDVVYCALGTTRGKAGKAGQYKVDYEYVVKLAQLSALQG
                     GKFLVVSSQGANARSAFFYMKTKGEMEEAVKKTGNRSIYIMR"
BASE COUNT         1936 a         1166 c         1114 g         1268 t
ORIGIN      
        1 agaatcgctg caaagcattg ccgatattac cggtggaaaa tatttcaggg caacaagcaa
       61 cgataaattg aaaatgattt acgaagaaat tgatgccctc gaaaaatcga agatcgaagt
      121 aaaggaattc agcaagaaac aagaagaatt ccagaaatat gcactaacag ccatgttgtt
      181 gcttgtagct ggcatattgc taaaaatgtc ggtattcaga aacataccgt agttgcctgt
      241 taaaaagttt cttagtgatt aaggaatttg tgaaggcaaa tgaaatatta caattgaaaa
      301 aaacataatt tcaggaataa ttaccctcat tatgagggct taggagaagc tcaaaatatg
      361 gaaatgttta gatttggaag tccgggttat ttatacgcac tgttgctaat tccagcactg
      421 gttgcgttat tcatatattt caggatacaa cgcaataaag cccttaggga atttgggaac
      481 cccgaaatcc ttgcgccact gatgccaggt gcttcgggta gccgcccaat atggaaattt
      541 gccatagtaa tgcttgccct tgcgttcctt atcacagggc tggctcgtcc gcagtttggg
      601 gcaaagcttc aaaagataaa tcgaaaaggc gttgaattga tcatagcgct cgatgtatcg
      661 aacagtatgc tggccgaaga catacagccc aaccgcctcg agaaagccaa aatggctata
      721 tcacgactta cagaacggtt gagcaatgac aaaattggat tggtagtttt tgcaggaaaa
      781 gcatatacac aaatacccat tactaccgat tatgcatcga ccaaaatgtt cctgaatgct
      841 atcaacacag aagtggtgcc ggtacaagga acagccattg gcgaggccat aaacctggcg
      901 atgagcagct ttactccaag tgaagaaacc agcaaagcta tcattgttat taccgatggt
      961 gagaaccacg aagacgacgc catagaaatg gcaaagcaag ccgcagaagc caaaataacg
     1021 gtacatacta ttggcatggg acttccgcaa ggcggcccaa taccaattta taatgcttac
     1081 ggacagaaag atttcaaacg tgacaaaagc ggacaggttg tggttacccg gctcgatgaa
     1141 aatatgctgg aacaaatagc tgctgcagga aacggaaagt acatccgtgc aaacaacacc
     1201 gaagtcggaa tcaacaacat attcgatgag attaacaaaa tggaaaaaac agaactcgag
     1261 tcgagaatct attccgatta caacgatcag ttcttctatt tcatcgcggt agcttttgcc
     1321 cttttattga ttgaattttt tgtattggaa agaaaaaaca atcttttcaa gaatattctc
     1381 caactaatta ggggcaaaga agagaaataa cgaaagcacg tcgacttaaa actataaaaa
     1441 gactatcata aagagcagaa aacggaggct gtcggaatat aaaaaagaaa ttatgaaacg
     1501 taactatttg cataccatag aaacaaaaaa caaaccaagc ataaaaattg gtaattgggg
     1561 atttacgacc attgtgcttc tattgttggt aagctcaatg tcggtttatg ggcagaaaga
     1621 acgcaaattc atcagacagg ggaacaagca ctacgaaaaa gcagtggcca atagcgactc
     1681 aacccacatc gactcggttg gattctcgaa tgcagaaatt gcttatagaa aagccctggc
     1741 caaaaaaccc ggcgattaca agtctcagtt taatattggg aatgcactct tcaaacagaa
     1801 caaaatggcc gaagcggctt cccaatacca gcaagtaata gaaggcggaa acaacaaaga
     1861 ggaaaaagga aaaaactggt tcaattacgg gaattcgatg ctggctcaac aaaaatttga
     1921 tgaaagcatt gatgcctata aaaatgccct gcgccaaaat cctaacgatg ttgaagccaa
     1981 atacaacctg gaatttgccc gcaggatgaa acagcaacag cagcaacagc agcagcaaca
     2041 gcaacaacaa gataaagacc aaaacaaaga tcagaataaa gatcaaaaca agaatcaaaa
     2101 caaagaccag aataaggacc agaacaagga ccagaacaag gaccagaaca aggaccagaa
     2161 caaggatcaa aaccaaaata aagatcagca acaacagcaa caacagcaac aatcaaaaat
     2221 atcgaaacaa aatgccgagc aattgctgaa agcgcttcag aacgacgaat cgaaaaccca
     2281 ggaaaaagtt aagaaagctg aagcacaaaa agcacagacg agacggcccg accaagactg
     2341 gtaacagtct cataacaaca aaaaacaggt gcttaaaaca agaatttata atacctttac
     2401 gcacttttta gaaaatgaca atataaagct gacaagcaaa aacataacaa cgatgaaccg
     2461 aaagatagtg tttataatta tgctcttcct tggcctgacc tccatggccg aagaggttcg
     2521 ttttaccatg tcgggacccg atatggtttc ggtaggcgaa caatttagcc tcactctttc
     2581 actcaatgcc gaaggcgaag atttgcggat gcccgatttg ccaaatttcg acatactcat
     2641 ggggccaagt atttctcaaa gtcgcagtta cagcgttgtc aacgggaaaa tgagccaatc
     2701 agtcaatttt agttatacct atattcttcg ggctcagagc gaaggaacat tcactattcc
     2761 accggcaagt gttaaatcga aacgggagac ataccaatcg aatcaggtaa caatcaaagt
     2821 gataaaaggc cggcaacaag ccaccacaca atcacaacaa gggaattcag cagccgggca
     2881 aaccaatagc ggccaagcca aaacaggtac cgtttcgcgc gataaccttt tcatgcaata
     2941 cgaagtagac aaacgcaacg tgtataaggg cgaaatgata ttggtagcgc tgaaactata
     3001 tagccgcgta agcctttctg tagccgatca aaccatacca tcgttcgaag ggttttgggc
     3061 cgaagacatt gaaattccat cggcagaaca gacacaaacc cgcgaagcag tcgacggaat
     3121 catctataac gtgtacaccc ttcagaaaaa aatattggtt cctcaacaaa caggtaccct
     3181 atacattgaa cccgccgaaa tggtctttaa cgttcagcaa cgcgtatcac cacaaagctt
     3241 gttcgacgat ttctttgggt cataccagaa tgtaagagta ccggtaaaaa ccaaccgaat
     3301 cagcatcaat gtaaaggaac tgccctcggc acctgcagga ttcaagggag ctgtcggcaa
     3361 attcagcctt agttcatcca tcgacaaaac caatatcaag tcgaacgaag caatcaccat
     3421 aaaaacaaaa atcagcggaa ccggaaacct gaaacatatt gcagcaccat catttagttt
     3481 tcctcccgat tttgaggtat atgacccgaa aaccagttat gattacaatg cttcacaatc
     3541 gggaatcaac ggatcgacta ctttcgaaca ggttttcatt ccccgttacg ctggaaaatt
     3601 caccatacca gccgagaatt ttgtatactt tgatccatca tctcagagtt acaaaaccct
     3661 atcgaccagt gaattcaata ttaatgttga aaaaggctcc gacgaccagt cgactacggt
     3721 tgtaagctcc atctcgaaac aagatgttaa atatatcggg aaagatatca ggtacattaa
     3781 gacagaaaac ccaagtctaa aaacccgcaa ccgtctgttt ttcggcactt tcaattacta
     3841 tttttcggta atagcagtct tgttggcctt tgctttcttc tttttcttcc agaaaaaaag
     3901 gattgcagaa aattcgaacc ttgcccttgt gcgtaaccgg caagcaagca aacttgcccg
     3961 caaacacctg aatgccgcat cgttgtgctt aaaaaacaat cagcgcgacg agtttttcga
     4021 agccctgtct cgtgccctat ggggatattt gagcgacaaa ctaacgctac ccttgtcaga
     4081 gctcaacaga gacaatgctc aagccacatt aagcaaatat tccgtagaag agaacacgat
     4141 ttctgaattt atccgaatca tcgacatctg cgaaatggca cggtttgcac ctacagcggt
     4201 gaacgaatca atttctgatt tatacggaga agcagaaacg ctgatttcga attttgaaaa
     4261 acaaatacgc aaaaaagcat aattatgaaa cgaatattca tcacaatact gctcattagc
     4321 ggttttttgg gagcgaatgc ccagcccacg aacctcttta aagaagcaaa caattattat
     4381 gctgcaaacg aatttgacaa agcagtaaaa agctatgaat caataatcaa ccaaggatat
     4441 gagtccgctc agttatattt caaccttgga aatgcctgtt ataaaaatgg agagttaaca
     4501 aaggccattc tttattatga acgggcaaaa ctgctgtccc caaacgacga agatattcaa
     4561 ttcaatattg atcttgtaaa ccaatttgtt gtcgataaaa ttgaggctct tccgcaacca
     4621 ttttttatta aatggggaca aggcatcgtt aatctgttca gctcaaatgg atgggcctgg
     4681 tttagtataa tcgtgctgat tatggcatta tcgggacttg gaatttatct tttctcaaaa
     4741 aacccgttgt tccgtcaaat cagttcaact actgcagtcg tcttgttttc tctttgcatc
     4801 ctgtcgatgg ttttcgcggg aaaacaaaaa gcaaaactca ccaaacacaa tgctgcaatt
     4861 gttttttcgt caacagttac ggtaaaatct tcgcccgaca atagcggaac agatcttttt
     4921 gttatacacg aaggactaaa agtgcagata aaagacgaag tgggcagttg gtacaacatc
     4981 cgccttgcag atggcaatag tggctgggta cagaaaacag ttcttgaaaa aatataaaaa
     5041 taccctacgc aaatgaaaac agcattgatt gctggagcca ccggacttgt tggaggagaa
     5101 cttcttaaac agctttcaga ggacaaacgg tatgaaaaga tcatagtact ctccagacgt
     5161 aagttaccag tacaatatcc aaaaacggaa gtaatcgtaa gttctttcga cactctcgag
     5221 agccttagcc taccctgccc tatcgatgtt gtatactgtg ctttgggcac tacacgcgga
     5281 aaagccggga aagccggaca atataaagtt gattacgaat atgtagtaaa actggctcag
     5341 ctgagtgcat tacaaggtgg caaattcctg gtagtaagct cacaaggtgc caatgcccgt
     5401 tcagcattct tctacatgaa aaccaaaggg gaaatggaag aagcggtgaa aaaaacaggc
     5461 aaccgcagca tctatatcat gcgt
//
LOCUS       DAOPUW010000032         5458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_14958_length_5458_cov_2.968536, whole genome shotgun sequence.
ACCESSION   DAOPUW010000032 DAOPUW010000000
VERSION     DAOPUW010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14958_length_5458_cov_2.968536"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..511
                     /locus_tag="PKJ43_01075"
     CDS             89..511
                     /locus_tag="PKJ43_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HNZ71178.1"
                     /translation="MEIIQLRDPNVLPDNEIIRGFLGNSFKAFSSLMDTLSSPEWGIV
                     ANWNYYNDGKAWLCKMVHKKKTVFWLSVWDGCFKTSFYFTEKNCTGLFDLGINEDIIE
                     DFNKNKPIGKLLPLVFEIRNEEQLYDLIKVIGYKKSIK"
     gene            560..1084
                     /locus_tag="PKJ43_01080"
     CDS             560..1084
                     /locus_tag="PKJ43_01080"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNZ71179.1"
                     /translation="MEFIELLQNRRSKRKFTTHKIEPDKVLLLQQAALMSPTGKSKNH
                     WEFIFIEASETLQRLSECKPHGAKLIANAPLAVVVIGDPQQSDTWVEDCSIASIIIQL
                     QAEALGLRSCWVQVHNRNHNETITAETFVKSLLNIDEGKAVLSIIAVGYSDEVRKPFN
                     IKELHTENIHKENY"
     gene            1273..2148
                     /locus_tag="PKJ43_01085"
     CDS             1273..2148
                     /locus_tag="PKJ43_01085"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ71180.1"
                     /translation="MPKGPKPLKGRLLNAYFVSTLSITLVLFLTGLLAMMIFNARYLN
                     NYIRENIGLTLVLNEDVRDVDLLQLQKIVTSQPEVKSATFIDSENAAKQMKDELGEDF
                     VSFLGYNPLNATIDVKLYAPYTVNDSLAQLETRFLGFPFVQEVYYQRNLVSLINENTK
                     KISLIIIGLAVIMLSIFITLINNTIRMSIYSNRFIINTMQLVGATRRFIRKPFVNRSI
                     LHGLIGGLLANVILVFLIFSYNKQFHEVINYNVNNLIFLVCGIVFFAGITITWISTTR
                     AVNKFLRMRYDELFY"
     gene            2155..2928
                     /locus_tag="PKJ43_01090"
     CDS             2155..2928
                     /locus_tag="PKJ43_01090"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNZ71181.1"
                     /translation="MNFIKRIFFKLLGVRAYLRLISRLFFFSYFNGFLKKKPDFECHY
                     MAQQLVKSGNFVIDLGANLGYYSVIFSKLVGNDGKVFSIEPVELFRNIFSKNTKKLTN
                     IEMLPYAIGPVDNEKIKMGVPVTSSYFSHGRTHVLNSAEECAMIFDAITMRPETIFKN
                     LDKLNYLKCDIEGFEGVAIPLFKEIILKFKPILQIEIDPENKDELVPFLKALDYSVFQ
                     VIDQKLHKVTDPHQAFGDLIFIDKRKIGKYVALLADKLF"
     gene            2994..3251
                     /locus_tag="PKJ43_01095"
     CDS             2994..3251
                     /locus_tag="PKJ43_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HNZ71182.1"
                     /translation="MIKFKSDNNSKETFALGKENYKLMIIGVGIIILGFLLMIGGGSD
                     NPNVFNPEIFSFRRITLAPLLVLGGLLFEIYAIMKKPKSNE"
     gene            3255..4040
                     /locus_tag="PKJ43_01100"
     CDS             3255..4040
                     /locus_tag="PKJ43_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNZ71183.1"
                     /translation="MSVIEAIVLGLIQGLTEFLPVSSSGHLEIGHALFGLDGGENLKF
                     DLLLHGATVLSTIVVFWKDIIGLIAGIFKFRWDDNNKFVAKLIISAIPVAIAGFLFEE
                     EIENMFSGNLIFIGAMLLITAILLSIANYAKKGIREIGFWDALLIGIAQMIAVTPGIS
                     RSGATIATGMIIGNDKTNLAKFSFLMVIIPIVGANFLKILSGDFSAGSSSVGLIPMIA
                     GFVTAFIMGLLACKWMINIVRKGKLIYFAIYCAAIGLIAIFAA"
     gene            4072..4776
                     /gene="truB"
                     /locus_tag="PKJ43_01105"
     CDS             4072..4776
                     /gene="truB"
                     /locus_tag="PKJ43_01105"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNZ71184.1"
                     /translation="MDQSLNGIDFQEGAVLLFDKPLDWTSFDLVNKVRWLLCKKLHIK
                     KLKVGHAGTLDPKATGLVIVCTGKATKTIDSLQAEEKEYIATLKLGATTPSYDLEKEE
                     DKTYPTEHITLELVQEKLKTFTGEIDQVPPIFSAIKVEGKRAFEFARKGNDPLLDARK
                     ITIYSIEVVRFEMPELVLRMNCSKGTYIRSLARDLGESLGSGAYLTGLIRTKSGAKML
                     ENAFTIEKFEKSLALI"
     gene            4835..>5458
                     /locus_tag="PKJ43_01110"
     CDS             4835..>5458
                     /locus_tag="PKJ43_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNZ71185.1"
                     /translation="MKLSKFKFKLPEELIAMHPAYHRDESRLMVLNRETQKIEHRIFK
                     EIIDYFDDGDVMIFNDTKVFPARLFGNKEKTGAEIEVFLLRELNREQRLWDVLVDPAR
                     KIRIGNKLYFGEIDLLVAEVIDNTTSRGRTLRFLYDGPYEDFKATLFGLGETPLPKFI
                     KRPVEEDDKDRYQTIFARNEGAVAAPTAGLHFSRELMKRLEIIGVDFS"
BASE COUNT         1762 a         1049 c         1060 g         1587 t
ORIGIN      
        1 attggcatca tctattctgt tgattcttgc cattgccgga ggtgctttct ttgaattcag
       61 aaaaaagaag taaaaactaa atatcattat ggaaataatc cagctccgag acccgaatgt
      121 tttacccgac aatgaaataa ttcggggatt tcttggcaat tcttttaagg cattttcatc
      181 attaatggat acattatctt ctcctgagtg gggaattgtg gccaactgga attattacaa
      241 cgatggcaaa gcctggttat gcaaaatggt tcataaaaaa aagacagttt tttggttgtc
      301 tgtatgggac ggttgtttca aaacatcgtt ctattttact gaaaaaaatt gtactggact
      361 tttcgacctg ggtataaacg aagatattat tgaagatttt aacaaaaaca agcctattgg
      421 caaattgctt ccgctggtct ttgaaattcg aaatgaagag caactctacg accttattaa
      481 agtgatcgga tacaaaaaaa gtataaaata gcgttttaac tatttttata ctgaaccgaa
      541 atcataaaac atcaaaggga tggaattcat tgaactttta caaaaccgac ggagcaaacg
      601 caaattcacg actcataaaa ttgaacccga taaggttttg ttattacaac aagctgcttt
      661 gatgtcgcca acaggaaaaa gcaagaacca ttgggaattc atttttatcg aagccagtga
      721 aacccttcag cgtttatcgg aatgtaaacc acacggagcc aaacttattg ccaatgcgcc
      781 attagccgtt gtggtaattg gcgatcccca acaatcggat acttgggttg aagattgctc
      841 tatagcctcc atcatcattc aattacaagc cgaagcactc gggcttcgaa gctgctgggt
      901 acaagttcat aataggaacc ataatgaaac cataactgcc gaaacctttg taaaatcact
      961 acttaatatt gatgaaggta aagcagtact ttccattatt gcggtcggct attcagatga
     1021 agttcgtaag ccgtttaata ttaaagaatt acacacagag aacatacaca aagaaaacta
     1081 ttgaaaacaa aacgatcgat gctgtaaagc cgtgatgcga cgttaatctt ttaatttcta
     1141 tcttagaaag aataaaaaag aaatccatag taaaactgac tttcgggttt cgatggctta
     1201 ctcaattatt tttatcactt ttgtagccgg agcaattttt attgaattca agaaacgtaa
     1261 aaagcagtaa gaatgcctaa aggaccaaaa ccactaaaag ggcggctgtt aaacgcctac
     1321 tttgtatcga cactgagtat taccttagtg ctcttcttga caggtttgct tgcaatgatg
     1381 attttcaatg cacgctacct gaacaattac atcagggaaa acataggcct tacgctcgtg
     1441 ttgaacgaag acgtacgcga tgtggatctt ttgcaattac aaaaaatcgt aacatctcaa
     1501 cccgaagtta aatcagcaac ctttatcgat tcagaaaatg ctgccaaaca aatgaaagat
     1561 gagttgggcg aagattttgt ttcttttctc gggtacaacc cactgaacgc caccatcgat
     1621 gtaaagctat acgctcccta tacagtaaac gacagcctcg cccaactcga aacccgtttt
     1681 cttggatttc ccttcgtaca agaagtttat tatcagcgta atttagttag tctgatcaat
     1741 gaaaacacta aaaaaatcag cttgattatt attggtcttg cggtaataat gctttcaatt
     1801 tttataacgc tcataaataa tacgatacga atgtcgattt actccaaccg gtttataata
     1861 aacacgatgc agcttgttgg agctacccgg aggtttatca ggaagccctt cgttaaccga
     1921 tcaatactac acggtcttat cggagggctg ttggccaatg taattcttgt gttccttatt
     1981 ttctcctaca ataaacagtt ccacgaagtg ataaattaca atgtcaacaa cctgattttc
     2041 ttggtatgtg gaattgtgtt ttttgcagga atcaccataa catggatttc gaccacacgg
     2101 gctgtgaaca aatttttgag aatgcgctac gatgaacttt tttactaaat tgagatgaac
     2161 tttattaaac gaatattctt taagctgctg ggagtcaggg catacttgcg cttaatcagt
     2221 cggctgtttt tcttctcgta tttcaacgga ttccttaaaa agaaacccga ttttgaatgc
     2281 cattacatgg cacagcaatt agtgaaaagt ggcaactttg taatcgactt aggcgctaat
     2341 ttaggatact attcggtaat tttttcaaaa cttgttggta acgacggtaa agttttcagt
     2401 attgaacctg tagaactttt ccgaaacatc ttttcaaaaa acacaaaaaa gctaaccaac
     2461 attgaaatgc tcccctatgc cattggcccc gttgacaacg aaaaaattaa aatgggagtg
     2521 cccgtaacca gttcttattt tagccatgga cgcacccacg tactcaacag tgccgaagaa
     2581 tgtgctatga tattcgatgc aataaccatg cgccccgaaa caatattcaa aaacctcgac
     2641 aaactaaatt acctgaaatg tgatattgaa ggattcgaag gggttgccat tcctcttttt
     2701 aaagagataa tcctgaaatt caaacccata cttcaaattg aaatagaccc tgaaaataaa
     2761 gatgaactgg tgcctttcct caaagccctc gattattcag tctttcaagt aattgatcag
     2821 aaattacata aggtaaccga tcctcaccag gcatttggcg accttatttt cattgacaaa
     2881 cgcaaaattg gaaaatacgt agcactcctg gccgataagt tattttaaga atgatactta
     2941 tctaaaagca gaatatttga ctatttttac accgctgatt aaaatgatat atcatgataa
     3001 aatttaaaag tgacaataac tcaaaagaga cctttgcctt aggtaaagaa aactataaac
     3061 taatgattat tggtgtaggc attattatct tgggtttcct gctcatgatt ggcggcggaa
     3121 gcgacaatcc caatgtcttc aatcccgaaa tcttcagctt cagaagaata accctggctc
     3181 cattattggt tttgggcggg ctacttttcg agatttacgc cataatgaaa aaacctaaat
     3241 caaacgaata acaaatgtcg gttatcgaag ctattgttct tggattaatt caaggactta
     3301 ctgaattttt gcctgtcagt tcaagcggcc atctcgaaat tggccatgcc cttttcggtc
     3361 tcgatggcgg agaaaacctc aaatttgacc ttttgttgca tggagctacc gtgctaagta
     3421 ctattgtagt attctggaaa gacattattg gtttgattgc cggaattttc aaatttcgat
     3481 gggacgacaa caacaaattt gtggctaaac ttatcatttc agccatcccg gtggccatcg
     3541 caggtttctt gttcgaggaa gaaattgaaa atatgttctc aggaaattta attttcattg
     3601 gagcaatgtt attgattact gcaatcctct tgtcgatcgc taattatgct aaaaaaggca
     3661 ttcgcgaaat cggattttgg gacgcattgc taattgggat agctcaaatg attgccgtta
     3721 ctcccggtat ttcacgaagt ggagccacaa ttgccacagg aatgattatt ggaaacgaca
     3781 aaaccaattt ggctaaattc tcttttctga tggttattat cccaattgtt ggtgccaatt
     3841 ttcttaaaat cttatcaggt gatttttcag ctggcagtag ttcagttgga ctgatcccaa
     3901 tgattgccgg cttcgttact gcattcataa tgggtttact tgcctgcaaa tggatgataa
     3961 acattgtgcg taagggaaaa ttaatatatt ttgctattta ttgcgcagct atcgggctta
     4021 ttgctatctt tgccgcctaa ttttttaggc aaaatagcaa ggtaaatatc catggatcaa
     4081 tcattaaacg gaatcgattt tcaagaaggg gcagtccttt tattcgacaa gcctttagat
     4141 tggacttcgt tcgatttggt gaataaagta cgatggttgt tatgcaaaaa gctgcatatc
     4201 aaaaaattga aggttggaca tgccggaacc ctcgacccaa aagctaccgg attggttatt
     4261 gtttgtaccg gaaaagccac aaaaacaatc gatagtttgc aagccgagga aaaagaatac
     4321 attgccacat tgaaactggg cgccacaact ccttcatacg acttggaaaa agaagaagat
     4381 aaaacgtacc caactgaaca tattacactc gaacttgttc aagagaaatt gaaaacattc
     4441 actggagaaa tagatcaggt accgccaata ttttcggcca taaaagtaga gggaaaaaga
     4501 gccttcgaat ttgcccgaaa agggaacgac cctcttctcg atgcacgtaa aattacaatt
     4561 tactcaatcg aggttgttcg tttcgaaatg cccgaactcg ttctcagaat gaattgcagt
     4621 aaaggaacat atatacgatc gttggctcgt gaccttggtg aaagccttgg aagcggtgca
     4681 tacttaacag ggcttataag aaccaaaagc ggtgcaaaga tgctcgaaaa tgcatttaca
     4741 atagaaaaat ttgagaaaag tctggcttta atctaagcaa ttgactattt ttcaaacaat
     4801 ttcgattcag aaaattatag aaagaatata aaatatgaag ttatctaagt tcaaatttaa
     4861 gctgccggaa gaattaattg caatgcatcc ggcatatcac cgtgacgaaa gccggttgat
     4921 ggtactaaac cgtgaaacgc aaaaaattga acacaggata tttaaagaaa tcatcgacta
     4981 cttcgacgat ggtgatgtga tgattttcaa cgacacgaag gtttttcctg cacgtctttt
     5041 tgggaataaa gaaaaaaccg gcgctgaaat tgaagtattc ctgctcaggg agctaaaccg
     5101 ggagcaacgc ctttgggatg ttcttgtcga ccctgcccga aaaatcagga ttggcaataa
     5161 actctatttt ggcgaaatcg accttttggt tgccgaagtt atcgacaata cgacttcaag
     5221 aggccggact cttcgttttc tatacgacgg accgtatgaa gatttcaaag caacgctttt
     5281 tggattggga gaaaccccgc tgccaaaatt catcaaacgc cctgtagaag aagacgataa
     5341 agaccgttat caaaccattt tcgcccgtaa cgaaggagct gttgctgcgc caactgcagg
     5401 acttcatttt agtcgggaac tgatgaaaag gcttgaaatt attggagtcg atttcagt
//
LOCUS       DAOPUW010000033         5452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_14976_length_5452_cov_3.430610, whole genome shotgun sequence.
ACCESSION   DAOPUW010000033 DAOPUW010000000
VERSION     DAOPUW010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14976_length_5452_cov_3.430610"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PKJ43_01115"
     CDS             <1..408
                     /locus_tag="PKJ43_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNZ71186.1"
                     /translation="QGKVDEQQMTYVIIASRYITSSWIFVRHRERNTWELPAGHIEPG
                     ETAMEAARRELFEETGAIDYKIRALFDYSAWHNGKQGYGRVYFAEVETLGKLPEFEIA
                     EITMQQTLPEELTYKNIQTTIFEEVKRMIKNND"
     gene            423..1247
                     /locus_tag="PKJ43_01120"
     CDS             423..1247
                     /locus_tag="PKJ43_01120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ71187.1"
                     /translation="MKTPEIPFLISPFDTAYLPQLEKLPPEEWQSNAYELFLHYDWQP
                     WFLAYQCIVNEQLAGFGMLMIFDEFAWFGWILVAPPFRRKGLGTAITQRLIDEATSRG
                     AKGLILTATEMGKPVYEKFGFTIDSSYLAFSLPHNYHPTFKKERIRRATSNDLEIICD
                     LDQQATGEQRKELIKGRLDDCFVYDDQKIEGFYMPSLGNGFIVACHKEAGITLLNYRC
                     TTDKRAIVVPEQNLDIVDYMRKKHFTEGFAIPRMTLGKRPDWRPSMIFSRAAGYLG"
     gene            complement(1287..2645)
                     /locus_tag="PKJ43_01125"
     CDS             complement(1287..2645)
                     /locus_tag="PKJ43_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNZ71188.1"
                     /translation="MHIHFIAIGGSAMHNLAIALHKKGYRVTGSDDEIFEPSKSRLEK
                     YGLLPAVQGWQPQLIEGRPDVIILGMHARKDNPELLKAQELGLKIYSYPEYLFEQTKT
                     KTRVVVGGSHGKTTTTSMIMHVLKFCSVNFDYMVGATIDGFDTMVNLDNDTEIAVFEG
                     DEYLSSPIDLRPKFHHYKPNIALLTGIAWDHINVFPTFEGYVDQFGIFASMIEPDGSL
                     IYFEGDENLRAIANQLPANVDAIPYSTHQHFVEGTTTFLVNGAKKFPLHVFGDHNLQN
                     ISGAYLVCKKLGISDEQFYKAISAFTGSSRRLEVVAKNDQCTVFYDFAHSPSKLKATT
                     MAVKKQFSDRKLVAVMELHTFSSLKREFLPQYHLSMECADVAYVYFDAHTIEHKKLEP
                     ISASDVEDAFGGKNCKVFTNSDLLFEQLRNTEWHNCNLLIMTSGNFSGKNIKEFAQAL
                     IK"
     gene            2729..3574
                     /locus_tag="PKJ43_01130"
     CDS             2729..3574
                     /locus_tag="PKJ43_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ71189.1"
                     /translation="MEIFTIDAGTFLCDGGGIFGGVPKLLWERQIKADHRNLIPLAMR
                     CLLVVSGERKILIETGAGDKLNKKFVENNGISNPHRLLESLEENGFSPSEITDVLHTH
                     LHWDHCGGGTTFDEKKQAVLTFPNATYHCARSQWENAMNPTPRENDAFFEEDLLPVQK
                     SGKLNLINDNCELIPGLELRIFNGHTPGMIVPIISYQGRKIAYVTDLTPLTANIPLKW
                     ISAYDLYPVTAMEEKLSFLREAHENKYILFFEHDTQTCCASLRWDEKKGPLVERKGDL
                     QSFIG"
     gene            complement(3593..>5452)
                     /gene="sprA"
                     /locus_tag="PKJ43_01135"
     CDS             complement(3593..>5452)
                     /gene="sprA"
                     /locus_tag="PKJ43_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNZ71190.1"
                     /translation="NINLTGQLTLTTLYNKVPFLKNINQKYGSSTRRYGSRGRAEQNR
                     RREETQNSQSTGKTKQVSFKEEKVSLKANVPKSIFHKLGTEKVNVVVLSAKGDTIKGT
                     SVVVNENRINFKSATDETNVTVTINGTIENVESFGQKALERTVRFLTGIQSVRVSYTN
                     SGGTEMPGFLGEPQAFRFGSQQLPLPGGGLSLAPGVPFLLGWQDENFALTAANKGWIS
                     KDTTTLKQFLSQSTETWNLGLTFEPFANFRIEFSGTRRESSNISSFVQYNSTKGAFEQ
                     FSRKETGNFDMSVLTIGTAFREGLGDPNSELFNKFKNENRQIIQRRLNEQRGFVAGVG
                     YSKSTVPGDTTNGISLNSSDVVIPALLATYCGIDASKMPLTARPGISWIRPNWRINYN
                     GDPQNISWLKDKVTSLNFSHSYRSTYAVGNFETNLDYRPDQSGFSWVRSQLDQGENYF
                     VPELDITAITIQENFSPLINLDVSFVNDFSASFQISKSRTMSFSFANMQLSEMKKNEF
                     TVGVGYRFTGLDMIIKTKRKSEKVSNDVNMRLDISSSNYKTFFRKIDEKGLMQSGTHL
                     YTVDFQSDYMVSDKLTLKLYYKFDFHDPHLKGGAEGYLQKNTEFGLSFNYAIM"
BASE COUNT         1659 a         1162 c         1112 g         1519 t
ORIGIN      
        1 cagggaaaag tagatgaaca acagatgact tacgtcatca ttgcatcgag atacattact
       61 tcgtcatgga tttttgtcag gcaccgtgaa cgtaatacat gggaattacc tgcaggccat
      121 attgaaccag gtgagacagc catggaggct gcccggcgtg aacttttcga agaaacaggt
      181 gcaatagatt ataaaatcag ggcacttttt gactattcgg catggcacaa cggaaagcag
      241 gggtacggac gagtttattt tgccgaagtg gaaacactgg gcaaacttcc cgagtttgaa
      301 attgcggaaa taaccatgca acaaaccctt cctgaagagc taacatacaa gaacatacaa
      361 acaactatat ttgaagaagt aaaaagaatg atcaaaaaca atgattgaat gccagttccc
      421 aaatgaaaac accagaaatt ccttttttaa tatcgccatt cgacacagcg tatctgccac
      481 aactggaaaa acttcctccc gaggagtggc agagcaatgc ctatgagctg tttcttcact
      541 acgactggca accatggttc ttagcttatc aatgcatagt aaacgaacag ttagccgggt
      601 ttggaatgct gatgattttc gacgaatttg catggttcgg ctggattttg gtcgctccgc
      661 cattcagaag aaaagggtta ggaacagcca ttacccaacg attaatagat gaagcaacat
      721 cgcgaggggc aaaaggctta attctaaccg ccactgagat gggcaaaccc gtatatgaaa
      781 aatttgggtt taccatagac tccagctatt tggcattcag ccttccgcac aattatcatc
      841 caacattcaa aaaagagaga atccggaggg caacaagtaa cgatttggaa ataatatgcg
      901 acctggatca gcaagccacc ggagaacaac gaaaggaact tataaaaggc cgccttgatg
      961 actgttttgt ttatgacgat caaaaaatcg aaggatttta tatgccttcc ctcggaaatg
     1021 ggttcattgt tgcatgccac aaagaggcag gaattacttt actgaactat cgatgcacaa
     1081 ccgacaaaag agccatcgtt gttcctgagc aaaatctgga tattgttgat tatatgagaa
     1141 aaaagcactt caccgaagga ttcgcaattc cccgtatgac cttaggcaaa agaccagact
     1201 ggaggccatc gatgattttt agcagggcag cgggatattt gggataacgc acatgtctcc
     1261 aacaaattaa atacacgcac ttactgttat tttatcagtg cctgggcaaa ttctttgatg
     1321 ttctttcctg aaaagtttcc tgaagtcata atcaacaaat tgcaattatg ccattctgtg
     1381 tttcttagtt gttcaaaaag gagatcagag ttggtaaata ctttacaatt tttccccccg
     1441 aaagcatctt caacatcact tgccgatatt ggttcaagtt ttttgtgttc aatggtatgc
     1501 gcatcgaaat aaacataagc gacatcggca cattccattg acaaatgata ctggggcaag
     1561 aactcgcgtt tcaagctact gaatgtatga agctccataa ccgcaacaag ttttctgtct
     1621 gaaaactgct ttttaacagc cattgtcgtt gcttttaatt ttgaaggaga atgggcgaaa
     1681 tcgtaaaaaa cagtgcattg atcgtttttt gcaaccactt cgagccggcg cgaagaaccg
     1741 gtaaacgcag aaatagcctt ataaaactgc tcatcggaaa tacccaattt cttgcataca
     1801 agataggctc ctgaaatatt ctgcaaatta tgatcgccaa aaacatgaag cgggaatttc
     1861 ttagcaccgt tcaccaaaaa agtagtagtc ccctcaacaa aatgttgatg tgttgagtaa
     1921 ggaattgcat caacattggc aggtaattga ttcgctatgg cacgaagatt ttcatcgccc
     1981 tcgaaataaa tgagagaacc gtctggctca atcatcgagg caaaaatgcc aaactggtca
     2041 acatatcctt cgaaagttgg gaaaacatta atatgatccc aggcaatgcc tgtaagcagg
     2101 gcaatattag gtttatagtg atgaaacttc ggacgcaggt cgataggcga agataaatac
     2161 tcatctcctt caaatacagc aatctcagtg tcattatcaa ggttcaccat tgtatcgaaa
     2221 ccatcaatag ttgccccaac catgtagtcg aaattaacac tgcaaaactt caacacatgc
     2281 attatcatcg aagttgtagt ggttttccca tgactaccac caacaacaac acgcgtttta
     2341 gttttagtct gttcgaaaag atactcgggg tatgaataaa ttttcaagcc aagctcctgt
     2401 gctttaagca actccgggtt atctttacgg gcatgcattc caagaattat cacatcgggg
     2461 cgtccttcaa taagctgagg ttgccagcct tgtactgcag gcaataaacc atatttttcg
     2521 agcctcgatt tcgatggttc aaatatttca tcatcggaac ccgttacgcg atagcctttt
     2581 ttgtgaagcg caatggccag attatgcatt gcactgcccc cgatagcaat aaaatgaata
     2641 tgcatgatgt ttgattttaa gtcgataaaa ataatgaaat aggtacatgt ttacttcatg
     2701 ttttctaata ttgcataaaa aagaaaccat ggaaattttc actatcgatg cgggcacctt
     2761 cctttgcgac ggaggaggta tttttggtgg tgtacctaag ttattatggg aacgacaaat
     2821 aaaagccgac catcgaaacc ttatcccgct ggctatgcgc tgcttgctgg ttgtaagcgg
     2881 tgaaaggaaa attcttattg aaactggcgc cggagataaa ctgaataaaa aattcgttga
     2941 aaataacggc atcagcaacc ctcataggct ccttgaatct cttgaagaaa atgggttttc
     3001 accatctgaa atcactgatg ttcttcatac ccacctccac tgggaccact gcggaggagg
     3061 aacaactttt gatgaaaaaa aacaagctgt attaacattc cccaatgcga cataccattg
     3121 tgcgcgttcg caatgggaaa atgccatgaa ccccacccct cgcgagaatg atgccttttt
     3181 tgaagaagac ttattgcctg tgcaaaaaag cggaaagcta aaccttatta atgacaactg
     3241 cgaattgata ccaggattgg aactacggat atttaacgga cacaccccag gcatgatcgt
     3301 tccaattatt tcatatcagg gaagaaaaat tgcttatgta acggatctca ccccgttaac
     3361 tgccaatatt ccgcttaagt ggatatcggc atacgacctt taccctgtta ctgccatgga
     3421 agaaaaactt tcgttccttc gcgaagctca cgaaaataag tacattttat tttttgaaca
     3481 cgatacgcaa acatgctgtg caagccttcg ctgggatgaa aaaaaaggtc ccctcgtcga
     3541 aagaaaagga gacctgcaat cttttattgg ataaattgat attgaatgtc aattacataa
     3601 tagcatagtt aaacgacaat ccgaattctg tattcttctg caaataccct tcggccccac
     3661 ctttcaggtg cgggtcatga aaatcgaatt tgtaatagag tttcaatgtc agcttatcgc
     3721 taaccatata atccgattgg aaatcgacag tatacaagtg tgttccgctt tgcataagac
     3781 ctttttcatc aatcttccgg aagaatgttt tgtaattact gctcgagata tcgagtcgca
     3841 tattaacatc attcgaaacc ttctcagatt ttcgtttggt cttaataatc atatcgagac
     3901 cggtaaagcg gtatccaact ccaacggtaa attcattctt tttcatttcg ctaagctgca
     3961 tattggcaaa actaaagctc attgtacgcg atttagaaat ctggaaactg gcactaaaat
     4021 cgttaacaaa agaaacatcg aggttgataa gcggactgaa attttcctga atggtaatgg
     4081 ccgttatatc gagttcaggc acaaaatagt tttcgccctg gtccaattga ctgcgcaccc
     4141 aactaaaacc actctgatcg gggcggtagt cgaggtttgt ttcgaaattt ccgacagcat
     4201 aggttgaacg atagctgtgt gaaaagttta acgaggttac cttatctttt aaccatgaga
     4261 tgttctgcgg atcaccatta tagtttatac gccagttcgg cctgatccac gaaattccgg
     4321 ggcgtgcagt aagcggcatt tttgatgcat caattccgca atatgttgcc aacaaggcag
     4381 gaataaccac atcggatgaa ttgagagaaa taccattggt cgtatcgccg ggaacagtac
     4441 ttttggaata accaacacca gcgacaaaac cacgctgctc attcagcctt cgctgaataa
     4501 tttgccggtt ttcgtttttg aacttattga aaagttctga gtttggatca cccagtcctt
     4561 ctctaaaagc agttccgata gttaacaccg acatatcgaa attaccggtt tcctttcggc
     4621 tgaattgttc aaaagcacct ttagtactat tatattgaac gaagctgctg atgtttgatg
     4681 attcgcggcg agtacctgag aactcaatgc ggaaattagc aaaaggctcg aatgttaacc
     4741 caaggttcca ggtttcggta ctttgagaaa ggaactgctt aagcgtagtt gtatctttag
     4801 aaatccatcc tttgtttgca gcagtaaggg caaaattttc atcttgccaa cctaaaagaa
     4861 atggtacgcc aggagccagc gacaagccac ctccgggaag gggaagttgt tgtgacccaa
     4921 aacggaaagc ctgtggttca cctaagaaac ctggcatttc ggttcctccg ctgttcgtat
     4981 aagaaacccg aacactctga atccctgtaa ggaaccttac ggtacgttcc aacgctttct
     5041 gtccaaaaga ctccacattt tcgattgttc cgttaattgt tacagtaaca ttcgtttcat
     5101 cagtagccga tttgaagtta atgcgatttt cgttcacaac gacagatgtt cctttaatgg
     5161 tatctccctt agcacttaac actacgacat taaccttttc agttcccagt ttatggaaaa
     5221 tcgacttggg cacattcgct ttcaaggaca ctttttcttc tttaaagctt acctgttttg
     5281 tttttccagt gctttgggaa ttttgcgttt cttctcttcg ccggttttgt tcagcgcgcc
     5341 cacggcttcc gtacctgcgg gtcgaacttc catatttctg gtttatattc ttcaaaaacg
     5401 gaactttatt gtacagcgtg gtaagggtaa gttgcccggt aaggttaatg tt
//
LOCUS       DAOPUW010000034         5421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_15084_length_5421_cov_3.426575, whole genome shotgun sequence.
ACCESSION   DAOPUW010000034 DAOPUW010000000
VERSION     DAOPUW010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15084_length_5421_cov_3.426575"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="PKJ43_01140"
     CDS             complement(<1..1280)
                     /locus_tag="PKJ43_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNZ71191.1"
                     /translation="MLGSIIAIIVYLFVITFLGYLGFKHTKTSTDYLIGGRQIHPMIM
                     ALSYGATFISTSAIVGFGGAAGTLGMGLLWLTVLNILFGIFIAFVVFGKRTRRMGHNL
                     DAHTFPELLGNRFNSKFIQGFAGVVIFLFMPIYTAAVLKGGADFIQTHFGISYEVSLL
                     FFAAVVAVYVWMGGMKGVMYTDAFQGAIMFIGMAFLIIFVYNKLGGITNAHEQLNQLY
                     NKPEIQEQTAGLIKGGFRGWTSMPASGSPIWWSLVTTIVMGVGIGVLAQPPLVVRFMT
                     VKSNRELNRAVVSGGVFILMMTGVAFVVGSLSNVLFFNETGKVAILAAGGNDKIIPMF
                     IKQFVPSWFGIVFFLALIAAAMSTLSSQFHAMGTAVGRDVYEKALGKKGNSILITRTG
                     ILLAILLSVILAYLANKLDSSMQIIATGTALFFGL"
     gene            complement(1292..1471)
                     /locus_tag="PKJ43_01145"
     CDS             complement(1292..1471)
                     /locus_tag="PKJ43_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71192.1"
                     /translation="MFGIHDIWIILAYVLCILSAILCVVYGIVNWNKGADLEPNELAE
                     ESDWSKNESEIEYKL"
     gene            complement(1503..1943)
                     /locus_tag="PKJ43_01150"
     CDS             complement(1503..1943)
                     /locus_tag="PKJ43_01150"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71193.1"
                     /translation="MAYQISIFLENKVGRLEKVTSILREASINIRAMNLTHTTQGWGI
                     LNLLVSNPEKAKEMLSAQNVPVSLRKIAVIPMDDVPGGLDDLLKKIAAVGINFTNAYG
                     RNTAPGKEAYLAIDVEDIENACEKLAAGGLKLLPDEKVYGNGTT"
     gene            complement(1955..3259)
                     /locus_tag="PKJ43_01155"
     CDS             complement(1955..3259)
                     /locus_tag="PKJ43_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNZ71194.1"
                     /translation="MLYWEKEIETLSRKGIEKVQVEKLNHIIGVAMQSPYYGNLYAGM
                     GIKPGAIDKIEDVRKLPFTTKQDLRNNFPWGFLSIDKKDVVRLHSSSGTTGNPTVIFH
                     SKEDLSNWANLVARSLFTAGIRDTDVFQNICGYGLFTGGLGFQYGIERLGALSIPAGA
                     GNSLRQIKLMMDYETTAAHAIPSYLTRLYDVFVSEGLDPRRDTKLHTFVIGAEPHSEE
                     HRRRIEEMFGVKAYNSFGLSEMNGPGVAFECAYQNGLHIWEDAYIVEIIDPISLEPVA
                     DGEVGELVMTSLDRRAMPLIRYRTRDLTRFLPESCPCGRTHRRLDRISGRSDDMFIVK
                     GCNVFPMQIEGVLMKIPEVGTDYLITLETIKDNDELIVEVEVKREWFSGDLDKLDRLT
                     REINRRIRDEVLVSPIIKLVEPGSLPQSEGKAVRVIDKRVKI"
     gene            complement(3276..4565)
                     /locus_tag="PKJ43_01160"
     CDS             complement(3276..4565)
                     /locus_tag="PKJ43_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71195.1"
                     /translation="MKRTFLLIMTLLSMFCAKGQSDDNLTLDFYGFVRVDGYVDTYKG
                     VDAGHDIFYLLPVYQVKNGAEYNKQLSSNISAMASRLGVKVTFPEVLKAKATGSLEFD
                     FAGNLKNDPTLFRIRQAYSLLTWEKSSLLVGQTWHPFFGGSCLPTVAGLNTGAPFTCF
                     NRSPMIRYNMKFGGLTLSASAVSQLQYNNFGIDAAEFSVTANQAKRNGVIPELVLSAE
                     WTKDAITLGAGALYNQIKPRMLITETLVDGKDASKIADEFLGSNAFMAYGRYKKDKLT
                     LLLKGYIGDNMQHLTLQGGYGVASFDSTSGKETYTSYKTYTAAFNLVYGQKWQVGLFA
                     GYGGNLGTNDALYNFGGKAKAYGLFTTVQSMYRVAPHISLNLPKFKLVAEIERTAANY
                     GTNNSDGSSSFDFTNGLYSATHQAVNNRVILVMTHFF"
     gene            complement(4849..5340)
                     /locus_tag="PKJ43_01165"
     CDS             complement(4849..5340)
                     /locus_tag="PKJ43_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71196.1"
                     /translation="MAIFNRDGRMIIPLRERNRKAVRANENKVRYIISEAFCPSGCSI
                     IDKEHEINGFPGLRLKFKRDGEEGEFVISAIEGDFEKIVLSGRLLEGVRDDLFCPHCG
                     AEFKKLVNCNCQNGAEMVVIGLTPELNYNDAITFCNVTGCSNGAFIKSGEAIRHVRLA
                     HGF"
BASE COUNT         1725 a         1229 c          936 g         1531 t
ORIGIN      
        1 aggccaaaga aaagagcagt accagtagca ataatttgca tgctcgagtc gagtttattg
       61 gccaaatagg caaggatcac acttaacagg atcgctaata agattcctgt tcgagtaata
      121 agtatagaat ttcctttctt tcccaaagct ttttcataaa catcacgacc aacggctgtt
      181 cccatagcgt ggaactgtga actcaaggta ctcatcgcag cagcaattaa ggcaagaaag
      241 aaaacaattc caaaccatga cggtacaaat tgtttaatga acattgggat aattttgtcg
      301 tttcctcctg cagccaaaat agccacctta cctgtctcat tgaagaacaa tacattagaa
      361 agagaaccaa ccacaaaagc aacaccagtc atcattaaaa taaatactcc tcccgatacc
      421 acagcgcggt tcaattcgcg attactcttt acagtcataa agcgcactac caaaggaggt
      481 tgagccaaca ccccaattcc tacgcccatt acaattgttg taaccaacga ccaccagatc
      541 ggagacccgg aggctggcat agaagtccat cccctaaaac cgcctttgat taagccagcg
      601 gtctgttctt gaatttcagg cttattataa agttgattaa gctgttcatg tgcattcgta
      661 atacctccta atttattgta aacaaatata atgaggaaag ccataccaat aaacataatg
      721 gcaccttgaa aagcatcggt gtacataacc cccttcatgc ctcccatcca aacataaacg
      781 gccactacgg cagcaaaaaa cagcaacgac acttcatatg aaataccgaa atgtgtttga
      841 atgaaatcag cgccaccttt caatactgca gcagtataaa taggcataaa caagaaaatg
      901 acaactcctg caaatccttg aataaactta gaattgaatc ggttacccag taactctgga
      961 aaagtgtgcg catcgaggtt gtgtcccatg cgacgggtac gtttcccaaa aaccacaaaa
     1021 gcgatgaaaa ttccgaacaa aatattcaga acagtaagcc ataacaatcc catgccaaga
     1081 gttccggctg ctccgccaaa accaacaatt gccgaggtgc tgatgaaagt agcaccatag
     1141 gaaagtgcca taatcattgg atggatttgt cgccctccaa ttaaataatc agtcgatgtt
     1201 ttagtatgtt tgaaaccaag atatcctaaa aacgtaatca caaaaaggta tacgataatt
     1261 gcaataatac ttcctaacat atagcagcct cctataattt gtattcgatt tcagattcgt
     1321 ttttactcca atccgattct tcggcaagtt catttggctc aaggtcggca cctttattcc
     1381 agtttacaat tccataaaca acacacagaa ttgcacttaa aatacacagc acataggcca
     1441 gtattatcca gatgtcatga atcccaaaca tacattcagt tttagattta ttatacagtt
     1501 aattaagtag ttccatttcc gtaaactttt tcatcgggca ataacttgag ccctcctgcg
     1561 gctaatttct cacatgcatt ctcaatgtct tcaacatcga tagctaaata agcttcttta
     1621 ccaggagcag tattgcggcc ataggcattg gtaaagttga tcccgactgc agcaattttt
     1681 ttcaaaagat catcaagccc gcccggaaca tcgtccatgg ggataacagc tatttttcga
     1741 agagaaacag gaacattctg agctgacagc atttctttag ccttttcggg gttactgacc
     1801 aacaggttaa gaattcccca tccctgtgta gtatgggtca aattcatagc cctaatatta
     1861 atcgatgctt cacgcaatat gctggttact ttttcaagcc ggccaacctt gttttcaagg
     1921 aagattgata tttgatatgc catgatttac aattttaaat tttcactctt ttatcgatta
     1981 ccctcacagc tttgccttcc gattggggaa gggaacctgg ttcaacaagc ttaataattg
     2041 ggctcacaag tacttcgtct cgaatacgac gatttatttc acgagtcaaa cgatcaagct
     2101 tatcaagatc acccgaaaac cactcgcgtt ttacttcaac ctcaacaatt aattcatcat
     2161 tatctttaat tgtttcaaga gtaatgaggt agtcggttcc cacttcaggt attttcatca
     2221 acacgccctc aatttgcatt ggaaaaacgt tgcatccttt tacaataaac atatcgtcgg
     2281 aacgcccgga aattcgatcg aggcgacggt gtgttcttcc acaggggcaa ctttcgggca
     2341 agaaacgggt caaatcgcgt gtacggtaac ggataagggg cattgccctg cggtcgagcg
     2401 aggtcattac caactctccc acttcaccgt cagctactgg ctcaaggctt attggatcga
     2461 taatttcaac aatgtaggca tcttcccaaa tatgaaggcc attttgatat gcgcactcaa
     2521 aagccacgcc cggaccattc atttctgaca aaccaaatga gttatacgct tttacgccaa
     2581 acatttcctc aatacgcctg cgatgttcct ctgaatgggg ttctgcccca attacaaaag
     2641 tgtgcagttt ggtatcgcgg cgaggatcaa ggccttcaga tacaaaaaca tcgtaaagac
     2701 gggtaaggta actcggaata gcatgtgctg ccgtagtttc ataatccatc ataagcttaa
     2761 tttgcctcag gctgttccct gctcctgccg gaatgctcaa tgctccaagc ctttcaattc
     2821 catactgaaa accaaggccc cccgtaaaaa ggccatagcc acagatattc tgaaaaacat
     2881 cagtgtcacg aattcctgcg gtaaaaagcg aacgggcaac caagttggcc caattcgaca
     2941 agtcttcctt tgaatgaaaa attaccgtag gattcccagt tgtacccgat gaactgtgca
     3001 aacgcacaac atccttctta tcgatggaca aaaaacccca aggaaaatta ttccgtaaat
     3061 cttgttttgt agtgaatgga agttttcgta catcttcaat tttatcaatt gcgccaggtt
     3121 taattcccat cccggcatac aaatttccat aatacggtga ttgcatcgca accccgataa
     3181 tatggtttag tttttcgact tgaaccttct ctattccctt tctcgagagg gtttcaatct
     3241 ctttttccca atataacatt tttaaaaaat taatattaaa agaaatgagt cattacaaga
     3301 atgacccggt tatttacagc ttgatgtgtt gctgaataca gtccgttggt aaagtcaaaa
     3361 ctagaactgc catcagagtt attagttccg taattagctg ctgttctttc tatttcagca
     3421 accaatttga attttggaag atttaatgaa atgtgtggag ctacccgata catactttgc
     3481 actgttgtga atagcccata tgcctttgct ttcccgccaa agttatatag tgcatcatta
     3541 gtgcctaggt ttcctccata accagcgaat aatccaacct gccatttctg accatataca
     3601 agattaaatg ccgcagtgta ggttttgtag ctggtatatg tttcttttcc ggaagtagaa
     3661 tcgaaagagg ctacgccata cccaccttgc aatgtaaggt gttgcatatt atctccaata
     3721 tatcctttta aaagaagggt caacttatct tttttgtagc ggccataagc catgaacgca
     3781 ttactgccaa ggaattcgtc ggcaattttg cttgcatctt tgccgtcaac aagggtttcg
     3841 gtaattaaca tccggggttt aatttggtta tataaagccc ctgcgccaag agtaattgca
     3901 tcttttgtcc attcggcact taaaactaat tcaggaataa ctccattgcg tttagcttga
     3961 tttgctgtaa cactgaattc tgccgcatca attccaaaat tattatattg taactggcta
     4021 acagcagaag ctgagagtgt taatccgcca aatttcatat tatacctgat cattggcgaa
     4081 cgattgaaac atgtaaacgg tgctcccgta tttaaccccg ctacagtagg taaacaactc
     4141 cctccaaaaa aaggatgcca ggtttgcccc accaagagcg acgatttttc ccaagtcaac
     4201 aatgaatagg cttgccgaat acgaaacaat gtaggatcgt tttttaaatt tccggcaaag
     4261 tcaaattcca acgagcctgt tgctttggct ttcagcactt caggaaaagt aaccttaacc
     4321 cccaaacgag aagccatggc actaatattt gaacttaact gtttgttata ttcggcacca
     4381 ttttttactt gatacactgg aagcaggtaa aaaatatcat gaccagcatc aacacctttg
     4441 tatgtatcga catagccatc gaccctgacg aacccataaa aatcgagagt tagattgtca
     4501 tcagactgcc ctttagcaca aaacatcgac aataaagtca ttatcaacaa gaaagttctt
     4561 ttcataatag attcataaaa aatggtttat ataagattca aggttgaaat ttgcggataa
     4621 aattaataat tatcacaaaa acgacaactt ttttaatgat ttataaataa atactattgt
     4681 gaacttctga attgaaacaa aagaaattat atcaaaccga ctgctaaatg gaaagagttg
     4741 tttgctaaat aaaaaaacca tcaacgcaac cgactctcaa ttgcgttgat ggtataagga
     4801 gaaatgctta atcaaccgtt ggatcatgct ggccgtaagc aaaataaatt aaaaaccatg
     4861 agctaagcga acatgtcgta tggcttctcc cgacttgata aatgcgccat tagaacaacc
     4921 tgttacatta caaaaagtaa ttgcatcgtt ataattcagt tctggagtta agcctataac
     4981 aaccatttcg gcaccatttt ggcagttaca attcactaat tttttaaatt ctgcaccaca
     5041 atgcgggcaa aacaaatcgt cgcgaacccc ttctaataac ctgccactta gaacaatctt
     5101 ttcaaaatcg ccttcaattg ccgaaataac aaattcgccc tcttcgccat cccttttaaa
     5161 cttcagcctt aagccgggaa atccattaat ttcatgctcc ttatcaataa ttgaacatcc
     5221 tgaagggcaa aacgcttcac taatgatata acggacttta ttctcatttg cacgaactgc
     5281 ttttcggttt ctctccctaa gcgggataat cattctccca tctctattaa aaattgccat
     5341 aagatacagg tttttagctt attacaacag tttcaaaaca ccaagttaac ctttaatcac
     5401 aagaaaataa catcaaggat a
//
LOCUS       DAOPUW010000035         5412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_15114_length_5412_cov_3.133470, whole genome shotgun sequence.
ACCESSION   DAOPUW010000035 DAOPUW010000000
VERSION     DAOPUW010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15114_length_5412_cov_3.133470"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PKJ43_01170"
     CDS             complement(<1..409)
                     /locus_tag="PKJ43_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71197.1"
                     /translation="MEKYAVVNSRNLTKTLLYKIILTLLVLFFLFLFALSSNWFGEGS
                     ALETFFFKNESSVMTPISIGIVIIAVILSVVTRSRMRNPIPLGTLELDEQGYTFTGMD
                     NGQTRGTWNNARYVVFEFFSVAKMNNPKGCLNYL"
     gene            594..1937
                     /gene="purB"
                     /locus_tag="PKJ43_01175"
     CDS             594..1937
                     /gene="purB"
                     /locus_tag="PKJ43_01175"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNZ71198.1"
                     /translation="MSLNELTAISPIDGRYRSKVSSLEEYFSEYALIRYRVFVEIEYF
                     IALCEIPLPQLENFDFSIAPKLRAIVSDFSIEDAQRIKDIEKTTNHDVKAVEYFLKEK
                     FDNLGLKAYKEFIHFGLTSQDINNTAVPCSLRDALQDVYYPLLEKLIAKLEQYAEVWT
                     DVPMLARTHGQPASPTRLGKEIKVFVERLNVQTELLRNIPCYAKFGGATGNFNAHNVA
                     FPAIDWVQFANKFCSEHLQLERSQYTTQIAHYDNYGAIFDNIKRINTILLDLNRDIWT
                     YISMDYFKQKIKEGEIGSSAMPHKVNPIDFENSEGNIGIANALFEHLSAKLPVSRLQR
                     DLTDSTVLRNIGIPIAHTLIAFHSTLKGLDKLLLNVDAIHADLEKNWSVVAEAIQTVL
                     RCENYPNPYEALLKLTRTNTKIDKEAIHSFIDSLDVSKELKIRLKQITPQNYTGL"
     gene            2032..3852
                     /locus_tag="PKJ43_01180"
     CDS             2032..3852
                     /locus_tag="PKJ43_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ71199.1"
                     /translation="MKGLFYLLKRFIPPYKGWAISNIIFNILGAIFGAFSFLALSPIL
                     GILFGTQQLVTEPVDFALNVESVKQFLYYKMSMIISNEGQFRALYLIGFFMIIMVFLK
                     VGFTYLASFCMMPIRNGVVRDLRNKICKKLMSLPLGFFSNERKGDIMARIAGDVNEVE
                     NSIMNSLEMLIKNPVLILVSLVIMIYMSWSLTLFVLVMLPFAGLLTGRIGKGLKKPSR
                     KAQDKMGEILSTIDESLSGLTIIKAFNAEKKMEKRFAKETDDYYKTMNRLMWRNYLAH
                     PVSEFLGTTVIIIVLWYGGRLILKNEGGMAPENFITYLVFFYSIINPAKAFSSAFYSI
                     EKGMASVDRIDAILMADDKIKEKENALSKTEFNASIEFRNVSFAYHKDIIIKNINLTI
                     PKGKTVALVGKSGSGKTTFVKLIPRFWDVTSGDILIDGVNIKDIKLSHLRGLMGYVSQ
                     EPVLFNDTFANNIAFGVENATSEQITKAATVAHAHDFIIESASAYQTKVGDLGQKLSG
                     GQRQRVSIARAVLKNPPLLILDEATSSLDTESEKLVQEALQSLMKNRTSIIIAHRLST
                     IKNADLIVVFRKGEVVEQGTHDELLEKRGEYYHLHKIQNF"
     gene            4112..>5412
                     /gene="tssR"
                     /locus_tag="PKJ43_01185"
     CDS             4112..>5412
                     /gene="tssR"
                     /locus_tag="PKJ43_01185"
                     /inference="COORDINATES: protein motif:HMM:NF036394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssR"
                     /protein_id="HNZ71200.1"
                     /translation="MKKSILITGMLLMFLFSGVVSMGQSVVKNTPGKYMMPMDNASAA
                     VPGKAKEGELWVVFSDRPANPLYSDKGCNSATGKKLDFMKPMYVIDETETAIRVVDTE
                     DADVRGNLKPGATSRAAWVKKENMLLWKTCLKTRDVNLPEFKGGIFNKKAMVLNIITG
                     AQSASRSPEFYSHPRCNPSDSINSALVYQINYVYKETATAYLLGDIPEISDLVNDPQR
                     VRGWVLKSKTTAWNHRLAYELNWDEKAVAERRASQKRTKIYSDQAMGGTPLFQEDASF
                     YRSRAIGEVDRYPVLDVNNGVTKLGVIGDLRSESGKVLPSAEFAKIKHVIDSMSNSLR
                     NVNVMFVIDATSSMVQYSKAIQDAIKSTMRNLQKGKNSYRFGVVLYRDASEGNSNATH
                     YTRELSSNYNGVIQTLTRYMTPTYNKCNNDAEECVFYGIKKA"
BASE COUNT         1754 a         1160 c         1077 g         1421 t
ORIGIN      
        1 tgagataatt cagacaacct ttgggattgt tcattttggc aaccgaaaaa aattcaaaaa
       61 caacatatcg tgcattattc catgtacccc gggtttgccc attgtccatt ccggtaaacg
      121 tgtacccttg ttcatcaagc tcaagagtac cgaggggaat ggggttacgc atccggctac
      181 gtgtaactac ggataaaata acggcaataa tcacaatccc aatcgaaatc ggagtcatta
      241 cagaagattc atttttaaag aaaaatgttt ctagagcaga accttcgcca aaccaatttg
      301 atgacagggc gaacaagaac aagaagaaca agacaagaag agttaaaatt atcttgtata
      361 ataatgtttt ggtaaggttt cgcgagttta caacagcata cttttccatg atgtttattt
      421 ttttatgtga gttaatttcc agcaaattaa aaaaaacata agaaaaagca gcaaaagctt
      481 tcaaaaaaat taataatgaa gccttatcat tgttaaaaaa atgccctgaa cccgcaataa
      541 aagcggaaac cacaaaaaat tatatctttg tagctcgatt ttaaagaaca actatgagtt
      601 tgaatgaatt aacagccatt tcacccatcg acgggcgata ccgcagcaaa gtaagcagtt
      661 tggaagagta tttttcggaa tatgccctga tcagataccg ggtttttgtg gaaatcgaat
      721 attttatcgc cttgtgcgaa attccactgc cacagttgga aaacttcgac ttttcaatcg
      781 ccccaaaact aagggctatc gtaagcgatt tctccatcga agatgcacaa cggataaaag
      841 acattgaaaa gactacaaac cacgacgtga aagctgttga atattttctg aaagagaaat
      901 tcgacaacct cgggttaaaa gcatataaag aattcataca ctttggatta acttctcaag
      961 acatcaacaa cacagccgta ccttgttcat tgcgagatgc cttgcaggat gtttactatc
     1021 cgctgctcga gaagctgatc gccaaattgg agcaatatgc cgaggtatgg accgatgtgc
     1081 ctatgctggc ccgtacccac gggcaaccgg catctcctac ccggctggga aaagaaataa
     1141 aagtctttgt tgaacgtctc aatgttcaga ccgagttatt gcgcaacata ccctgttatg
     1201 ccaaattcgg gggcgctacc ggcaatttca acgcccataa tgtagctttt ccggctatcg
     1261 actgggtgca gtttgccaac aaattctgtt cagaacacct tcagctcgaa cgctcacaat
     1321 acacgacaca aattgcgcat tacgacaact acggggcaat tttcgataac atcaagagga
     1381 ttaacactat acttcttgac ctaaaccgcg atatatggac ctatatttcg atggactatt
     1441 tcaagcagaa aatcaaagaa ggagaaattg gctcgtcggc catgccacac aaagtaaacc
     1501 ccatcgactt cgaaaattca gaagggaata ttggcatagc caatgccctg ttcgaacatc
     1561 tttcggccaa attacccgta tcaaggctcc agcgcgacct gaccgactct acggtactgc
     1621 gaaacattgg catccctatt gcacacacat taattgcctt ccattcaacc cttaaaggcc
     1681 tcgataagtt gctgttaaat gttgatgcaa tccatgccga tttggagaaa aactggagcg
     1741 tagtagccga agccatacaa accgtgttgc gctgcgaaaa ttaccctaat ccatacgaag
     1801 ccttattaaa actgacaaga accaatacta agattgataa ggaagccatc cattcattca
     1861 tcgattcgct cgacgtttct aaggaattaa agatcagatt aaaacaaata actccacaaa
     1921 attataccgg attataattt gaaattgaag cgatcaacgg aaatttattg caaaaactca
     1981 caataaattt ccgttgcttc agttttactt cagaccacat aaccttcatc gatgaaagga
     2041 ttattctatc ttttgaaacg atttatccca ccctacaaag gatgggctat ttcgaacatt
     2101 atctttaata ttctcggggc tatttttggc gctttctctt tccttgccct cagcccgata
     2161 cttggaattc ttttcggcac ccaacaatta gtaaccgaac ccgtcgactt tgcattgaat
     2221 gttgaatcgg tcaaacaatt cctctactat aaaatgagca tgataatcag caacgaaggg
     2281 cagttcaggg cactgtacct cattggcttt ttcatgatta ttatggtttt tctgaaagta
     2341 ggctttacct acctcgccag tttttgcatg atgcccatcc gcaacggggt tgttcgcgac
     2401 ttacgcaata aaatttgcaa aaaattaatg tctttaccat taggcttctt ttcaaacgaa
     2461 cgaaaagggg acattatggc ccgcattgcc ggtgatgtca acgaggttga aaattcgatc
     2521 atgaactcac tcgagatgct cattaaaaat ccagtgctta tccttgtttc tctggtcatt
     2581 atgatataca tgagctggtc gcttacctta tttgtcctcg ttatgctgcc tttcgccgga
     2641 ttgttgactg gacgcattgg caaaggcctt aaaaaacctt caagaaaggc gcaggacaaa
     2701 atgggcgaaa tactcagtac aattgatgaa tcgctttcag gattgacgat tataaaagcg
     2761 ttcaatgctg aaaagaagat ggaaaaaaga ttcgctaaag aaactgacga ttactataaa
     2821 acaatgaacc ggctgatgtg gcgaaattac ctggcccatc cggtaagcga atttttgggt
     2881 acaacggtaa taatcattgt tttgtggtat ggtggccgct taatattgaa aaacgaaggc
     2941 gggatggccc ccgaaaactt catcacctac cttgtattct tttacagcat aataaacccc
     3001 gctaaagcct tttcatcagc attttacagc atagaaaaag gcatggcctc tgtcgatcgt
     3061 attgatgcta ttttaatggc cgacgacaaa atcaaagaaa aggaaaatgc cctaagcaaa
     3121 acagaattca acgcgagtat cgaattcagg aatgtgtctt ttgcttatca taaagacata
     3181 ataatcaaaa acataaatct caccatacct aaaggaaaaa ctgtggcgct ggttggcaaa
     3241 tcaggctctg gcaaaaccac ctttgtaaaa cttatcccca ggttttggga tgtaacttcg
     3301 ggcgatatcc tcatcgacgg ggtaaacata aaagatataa agcttagcca cttacgaggc
     3361 cttatggggt acgtaagcca ggagcctgta ttgtttaacg acacttttgc aaacaacatt
     3421 gcctttggtg tagaaaatgc taccagcgag caaattacca aagcagcaac tgttgctcat
     3481 gctcacgatt tcattataga atcggccagt gcttatcaga ctaaagttgg cgacctgggg
     3541 caaaaactct cgggcggaca acggcaacgc gtatcgattg cccgtgcagt actgaaaaac
     3601 cctcccttat taatcctcga cgaagcgacc tcatcgctcg ataccgaatc ggaaaaattg
     3661 gtacaggaag cactccaaag cctgatgaaa aaccgcacat ctataataat agcccaccgc
     3721 ctgtcgacaa taaaaaatgc ggatcttatt gttgtattcc ggaaaggcga agtagtagaa
     3781 cagggaacac acgacgaact actcgaaaaa agaggggaat attaccactt gcacaaaatt
     3841 caaaacttct aagaaacagg ttattaccga aacctctggt ttttcatcgt aaaaaaatga
     3901 aaaagtgtgg tatataattt gaaagaaacg aaactataat aaaagtaaag ttttatatta
     3961 taccatcttt cattactttt gattcttgct ttttttaaag caaaaattca attatttttg
     4021 attagtttaa cggtcaaaaa gatataaact ggttttttca acaaataaag atggtgatga
     4081 ataattaccg ttttttacaa aatatgtttt tatgaagaaa agtatactta ttaccgggat
     4141 gctcctgatg tttttattct cgggtgtggt ttctatgggg caaagtgtag taaaaaatac
     4201 tcccggaaaa tatatgatgc ccatggacaa tgcttcagcg gctgtaccgg gaaaagcaaa
     4261 ggaaggtgaa ttatgggtag tattttctga ccgcccggcc aaccccctgt attccgacaa
     4321 gggatgtaac tcggccactg gcaagaaact cgacttcatg aaacccatgt acgtgattga
     4381 cgagactgaa acggcaatca gggtagttga taccgaagat gccgacgtac gtgggaacct
     4441 aaagccaggt gctacttcga gggctgcatg ggtaaagaaa gaaaacatgc ttctctggaa
     4501 aacctgtctt aaaaccagag atgtaaacct tcctgaattc aaaggcggaa ttttcaacaa
     4561 aaaagccatg gtgctgaata tcattaccgg tgctcaaagc gcaagccgct ctcctgagtt
     4621 ctattctcac ccgcgttgta acccttccga ctcaattaat tcggccttgg tttaccaaat
     4681 taactatgtg tacaaagaaa ctgcaactgc ataccttttg ggcgatatac ccgaaattag
     4741 tgaccttgtc aacgacccac agcgcgttag gggctgggta ttaaaatcga aaaccactgc
     4801 atggaaccac cgtttagcct atgagcttaa ctgggacgaa aaagcagttg ccgaaaggcg
     4861 cgcttctcag aaaaggacaa aaatatactc agaccaggcc atgggcggca ctcccctctt
     4921 ccaggaagat gccagttttt atcgctcgag ggctattgga gaagttgacc gttacccagt
     4981 gcttgatgta aacaacgggg taacaaaact tggcgttatc ggagatttac gttcagaaag
     5041 cggcaaagtg ctgccaagtg ccgaatttgc taaaatcaaa catgttatcg actcaatgtc
     5101 gaactcattg cgtaatgtaa atgtgatgtt tgttattgac gcaaccagca gcatggttca
     5161 gtattcaaaa gccattcaag atgccataaa atcaaccatg cgcaacttgc aaaaaggaaa
     5221 gaacagctac cgtttcgggg tcgttctata ccgcgatgct tccgaaggca attccaatgc
     5281 tacgcattat accagagaat tatcatcgaa ctataacggg gttatccaaa cgctcacccg
     5341 ttatatgaca ccaacttata ataaatgtaa caacgacgca gaagaatgtg tattttacgg
     5401 gatcaaaaaa gc
//
LOCUS       DAOPUW010000036         5387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_15236_length_5387_cov_3.176669, whole genome shotgun sequence.
ACCESSION   DAOPUW010000036 DAOPUW010000000
VERSION     DAOPUW010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15236_length_5387_cov_3.176669"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1541)
                     /gene="guaA"
                     /locus_tag="PKJ43_01190"
     CDS             complement(12..1541)
                     /gene="guaA"
                     /locus_tag="PKJ43_01190"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNZ71201.1"
                     /translation="MQEKILILDFGSQYTQLIGRRVRELNVYCEIHPFNHFPEIDDTV
                     KGVILSGSPFSVRDAQGPRIDLSGIKGSIPLLGVCYGAQYMAHFFGGEVALSNSREYG
                     RANLNYVDTNNELFHGISTGTQVWMSHGDTINRIPSNYTVIASTNDVEYAAYKIENEK
                     TYAIQFHPEVYHTTEGSKLLFNFVVSICGCAQSWTPASFIETTVHNLKETLGNDKVVL
                     GLSGGVDSTVAAVLLNSAIGKNLTCIFVDNGLLRKDEFANVLDQYKGMGLNVIGADAK
                     AKFWNDLKGVTDPEKKRKIIGRDFIEVFDVEAHKIQDVKWLAQGTIYPDVIESVSVNG
                     PSATIKSHHNVGGLPEKMNLKIVEPLKSLFKDEVRRVGRELGINPKLLGRHPFPGPGL
                     GIRILSDITPEKVRILQEADAIFINALHEWGLYDSVWQAGVMLLPVQSVGVMGDERTY
                     ENVVALRAVASTDGMTADWVHLPYDFLAKVSNDIINKVRGINRVVYDISSKPPATIEW
                     E"
     gene            complement(1705..2721)
                     /locus_tag="PKJ43_01195"
     CDS             complement(1705..2721)
                     /locus_tag="PKJ43_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNZ71202.1"
                     /translation="MKPSFIKILLFTFLLGFLASSCSQMTGGVKVGFLIPATVGYRWP
                     IDQQYVEKIAADRKIEVITRSAENDENLQLKQAEELLKMGVDVLIVVAANANTAAAIV
                     REAHDYNVPVIAYDRLIKNADLDYLVTFEGAEIGNLMVNYALKVKPEGNYVLLWGDAS
                     DVNAIMIKDAQEAALKPSIDNGRIKIVYKSFTEDWSSDNAYYTMKNILDFSGEKIDAV
                     ITSYDGLAMGARKAIDEAGLDYDVILTGQDAEIAAVRAINQGKMSLTVYKSINTIAAA
                     SVDLAIKLAKNQKVTGVTATIDNGRKEVPTMMLKPVAVTKDNIREVLIADKFFTEEQI
                     YGSN"
     gene            2945..4720
                     /locus_tag="PKJ43_01200"
     CDS             2945..4720
                     /locus_tag="PKJ43_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNZ71203.1"
                     /translation="MTKKQRKLSLNGGLKKWNDFKIGTKIGIGFGLLIFLAALIGGIS
                     IVSMRQIQGETNTLANESLPSVNESFRLDKDWKEIMFYLQAYDYIRNPYYLDRANQYL
                     VKYSNSLDGLIKIAGKSKKLNISNEKLSSLKSSLGEYQTMISNYKELEMKNQKLLEEV
                     EAEIASLQQNGGAGNAVLSAGYEIMLSINKRKPHELDRAQDYLNKARNSGGNASVSRI
                     ISSGNQLITGYKKARELELKRNEMENLLKVDIMALADVGLDQITEMTETTNSIITSSR
                     LGLTFIMIVILVIGVILGIIISRSITSSIKMGLAIAQNISNGVLHRQREVSRKDEIGE
                     LMTALYRMNNQLRFIVEDISMGIQNIAVASQKMNKNASELSEAATDQAATTEEISSSI
                     EELHANFMQNADNAEETAKIALVTVDGIREGNKATELASESLNDIIEKVSFIGDLAFQ
                     TNILALNAAVEAARAGDHGRGFAVVASEVKKLADSSKQAAELINKVSRETVIASDQAG
                     IKLKKIVPEVEKTANLIQGIKLASAEQEVGINQINNAIQQLNNIAQQNAFSSENMASS
                     AQDLSNLSEKLKKTISFFKVGSEAS"
     gene            4852..>5387
                     /locus_tag="PKJ43_01205"
     CDS             4852..>5387
                     /locus_tag="PKJ43_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNZ71204.1"
                     /translation="MKAIVFAAGLGTRLRPLTDNCPKALVELNGKPLLWHAINTLKKA
                     GIDDVVVNVHHHANQIKEYLAHNDLGAIIAISDESDLLLDTGGGLLFAKKLLDGNEPF
                     LAINVDIISSVDLTDVIKYHTTNDALATLVVRNRKTSRLFLFDNKMKLSGWKNFDTGE
                     EKIATENFYKSKPLAFSG"
BASE COUNT         1752 a         1115 c         1004 g         1516 t
ORIGIN      
        1 acagcctttt tttattccca ctcgatggtt gcgggtggtt ttgaacttat atcgtacaca
       61 actcggttaa tccctcttac cttattaata atatcgttcg atactttagc caaaaaatca
      121 tacggaaggt gcacccaatc ggccgtcata ccatcagtag aagcaacagc tctcagcgcg
      181 acaacatttt cgtaggtgcg ttcgtcaccc ataacaccaa ccgactggac tggcaagagc
      241 attacgccag cttgccaaac agaatcgtat aaaccccatt catgtaaggc attaataaaa
      301 atagcatcag cttcttgtag tatcctaact ttctctggag taatgtcgct taaaatccgg
      361 attcccaatc caggaccagg aaatggatgg cgacctaata acttaggatt tatccccaat
      421 tcgcgcccta cccttcttac ttcatcttta aaaagcgatt tgagaggttc aactattttg
      481 agattcattt tttcgggcaa acctccaaca ttgtgatgtg attttatagt tgccgaaggg
      541 ccattaacag aaaccgactc gataacatcg gggtaaatgg taccttgtgc caaccactta
      601 acgtcttgta ttttgtgagc ttctacatcg aagacttcaa tgaaatcgcg accaataatt
      661 ttacgtttct tctcagggtc ggtaacacct ttcaagtcgt tccagaattt tgctttagca
      721 tcggcaccaa tcacattcag ccccatccct ttatattgat ccagaacatt tgcaaactca
      781 tcttttctca gcaaaccatt atcgacaaag atgcaggtaa ggttctttcc aatagccgaa
      841 ttaagtaata cggcagcgac agtagaatca acaccgcccg aaagcccaag aacaactttg
      901 tcgtttccca aggtttcttt caaattgtgt actgtagttt cgataaacga agccggggtc
      961 cacgattgag cgcaaccaca aatggatacc acaaagttga atagcaattt tgacccttct
     1021 gtagtatgat atacctcggg gtgaaactga attgcatatg ttttttcgtt ttctatcttg
     1081 taagcagcat attctacatc gttggtgctt gctataactg tataatttga tggaattcgg
     1141 ttaatcgtat caccgtgcga catccatact tgagtacctg ttgaaatgcc gtgaaaaagt
     1201 tcattattag tgtcgacata attgagatta gcgcgcccat attcacgaga attcgaaagg
     1261 gctacttcac ccccaaaaaa atgggccata tattgagcac catagcaaac cccaagtaaa
     1321 ggaattgacc ctttaatgcc actgagatcg atccgtggac cttgggcatc gcgtaccgaa
     1381 aatgggcttc ctgaaaggat aacgcctttt accgtatcat caatttctgg aaaatggttg
     1441 aacggatgaa tctcgcaata aacatttaac tcacgaaccc tacgcccaat tagctgggtg
     1501 tattgagatc caaaatcaag gatcagaatt ttttcctgca tgtttttatt tttcaaagtt
     1561 ggtgcaaata taaaccctaa agggaaatta ttaatattta atatcgggct tagtagaaaa
     1621 aagggcggaa tctttaatga accacccttt taatattgta tcaaataaaa tattcaaaca
     1681 tataatcacc taattataaa aaccttaatt agaaccgtaa atttgctcct ctgtaaagaa
     1741 tttatcagca ataagaactt ctcgaatgtt atctttggta actgcaacag gtttcagcat
     1801 catagtaggt acctcttttc ttccattatc gatggttgcc gtaactccag ttactttttg
     1861 gtttttagcc agcttgatag ccaaatcgac cgaagccgca gcaatagtat tgattgattt
     1921 ataaacagtt aacgacattt tcccctgatt gatagcccga acagcagcaa tctcagcatc
     1981 ttgacccgtt aagatgacat cgtaatcgag ccctgcttcg tcaattgcct ttcttgctcc
     2041 catagccaac ccatcgtaac ttgtaatgac tgcatcaatt ttctccccac taaaatcaag
     2101 aatattcttc attgtatagt aggcgttatc acttgaccaa tcttcagtaa atgatttata
     2161 aacgattttt atcctgccat tatcgatgct gggctttaga gcggcctctt gcgcatcttt
     2221 aatcattatg gcattcacgt cactggcatc gccccacaga aggacataat tgccttcggg
     2281 cttcactttt aaggcgtaat tgaccataag atttccgatt tcagcccctt caaaggtaac
     2341 caagtaatcg aggtcggcat tttttatcag tcgatcgtaa gcaattacag gaacattata
     2401 atcatgagct tcacgcacta tagctgctgc agtatttgca tttgcagcta caacaataag
     2461 cacatcaact cccattttca gcaattcttc ggcctgcttc agctgaaggt tctcatcgtt
     2521 ttcggctgag cgtgttatta cttcgatttt acgatcagca gcaatctttt caacatattg
     2581 ttggtcgata ggccatcgat atcctactgt ggctggtatt agaaagccaa ctttaacccc
     2641 gccagtcatt tgtgaacaag aagaagctaa aaatccaagc aagaaagtga atagaagaat
     2701 ttttataaaa cttggtttca tgataactaa tcttaaagtg tataaaataa ctaatttaga
     2761 aataaaactc ccgttgtatc aaatatagaa acaatgtttg aaaaactaga ataagttttt
     2821 gaaaaatcat tttttttttc aatttttata atttagttcg tagaataata aaaatcaata
     2881 ataaccttcg atatttccat ataattaaaa gactgaacgg taatttaatg taaagaaaga
     2941 aattatgaca aagaaacaac gtaaactttc actaaatgga ggtttaaaaa aatggaacga
     3001 ttttaagatt ggcactaaga tcggaattgg atttgggttg ctaatatttc tggctgccct
     3061 aatcggaggt atatctattg taagcatgag gcagatacaa ggcgaaacaa ataccctcgc
     3121 caacgagtcg ctgccttcgg taaacgaatc gttccgtctt gacaaagact ggaaagaaat
     3181 tatgttttac ctgcaagcct atgactatat caggaaccct tattatttag acagggcaaa
     3241 ccaatatttg gtgaaatatt ccaattcgct cgacggtctt ataaaaatcg ctggtaaatc
     3301 gaaaaaactg aacatttcaa acgaaaaact ctcttcgtta aaaagttcgc tgggcgaata
     3361 tcaaaccatg atttcgaatt acaaagaact tgaaatgaaa aaccagaaac tccttgaaga
     3421 agttgaagca gaaattgctt ctcttcaaca aaacgggggt gctggcaatg ctgttctgtc
     3481 ggctggatac gaaattatgc tttcaatcaa taaacgcaag ccacatgaac ttgaccgcgc
     3541 tcaagactac ctcaataaag cacgtaacag tggcggaaat gcttcggtta gcagaataat
     3601 aagctctgga aaccaactta ttacaggcta caaaaaagcc cgcgaacttg agttgaaacg
     3661 taacgaaatg gaaaatctgt tgaaagttga catcatggca cttgccgatg ttggcctgga
     3721 ccaaattaca gaaatgaccg aaaccaccaa ttcgattatc accagttcaa ggcttggcct
     3781 gacatttatt atgattgtaa ttttggtgat tggtgttatt ttaggaatta taatttcccg
     3841 ctcaataacc agttcaataa aaatgggcct tgccatagcc cagaacattt caaacggcgt
     3901 gttacaccgc cagcgtgaag tatcgagaaa agatgaaatt ggtgagttga tgacagccct
     3961 gtaccgcatg aacaatcaac ttcgctttat tgtagaagac atttcgatgg gaatacagaa
     4021 tattgccgtt gcaagtcaaa aaatgaacaa aaatgcttct gaacttagcg aagctgcaac
     4081 cgaccaagcc gctacaactg aagaaatatc gagctcgatt gaagaattgc atgccaactt
     4141 tatgcaaaat gccgacaatg ccgaagaaac agcaaaaatt gcgttggtaa ctgttgacgg
     4201 aatcagagaa ggaaacaaag ccacagaact tgccagcgaa tcgctcaacg acattataga
     4261 aaaagtatca ttcattggcg accttgcgtt ccaaaccaac atattagccc taaatgcagc
     4321 cgttgaagct gcgcgtgccg gcgatcacgg gcgtggtttt gcggtagtcg cttctgaggt
     4381 gaaaaaattg gccgacagta gtaaacaagc tgctgagttg atcaacaaag tgtcgagaga
     4441 aacagtaatt gcctctgatc aggctggtat aaaattaaag aaaattgtac ctgaagttga
     4501 aaaaacagcg aatcttattc aaggaatcaa attggccagt gccgaacagg aagttggtat
     4561 caatcaaata aataatgcga ttcaacagct gaacaacatt gctcaacaga atgccttcag
     4621 ttcagagaat atggcctcat cggctcaaga tttatctaat ttgtctgaaa aactgaaaaa
     4681 aaccatttcg ttcttcaaag ttggttctga agcaagttaa aaaagatatt aaataaatga
     4741 tacatatgag gttgcccata aatggcagcc tctttcttta ccattcatcc gtatcacaat
     4801 ggaattatta caccgataaa agtataataa gccacataaa tcaacctaca aatgaaagcc
     4861 atagtatttg cggccggatt aggtacccgc ttaagacctc taactgacaa ttgccccaaa
     4921 gctttggtcg aacttaatgg caaaccactg ctttggcatg ccatcaacac gttgaaaaaa
     4981 gccggaatag atgatgttgt tgtgaatgtg caccatcacg ccaatcagat aaaagagtac
     5041 ctagctcaca acgacttagg ggcaataatt gctatttctg atgaaagtga tctactgctc
     5101 gataccggcg gtggcttatt gtttgctaaa aaactactcg acggaaatga gccgttttta
     5161 gcaataaatg ttgatattat cagttcagtc gacttaacgg acgtaattaa atatcataca
     5221 actaacgacg ctcttgccac attagttgta cgaaaccgga aaacgagcag gttgttcctt
     5281 tttgataata aaatgaaact tagtggttgg aaaaatttcg atacaggaga agagaaaatt
     5341 gcaaccgaaa atttctacaa atcgaaacca ttggcattca gtggcat
//
LOCUS       DAOPUW010000037         5277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_15650_length_5277_cov_3.283033, whole genome shotgun sequence.
ACCESSION   DAOPUW010000037 DAOPUW010000000
VERSION     DAOPUW010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15650_length_5277_cov_3.283033"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKJ43_01210"
     CDS             complement(<1..222)
                     /locus_tag="PKJ43_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71205.1"
                     /translation="MLKYKTARLSILLIIVFSIFSLNNATAQIKWSVLTTNGSWPGLS
                     DPRAIFIPGDTSHVILGYVTEDGAIASMLF"
     gene            complement(236..1657)
                     /locus_tag="PKJ43_01215"
     CDS             complement(236..1657)
                     /locus_tag="PKJ43_01215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNZ71206.1"
                     /translation="MKTDIFLYNKHLFNDNTHFNGWVISPDNSTDEVSHIFSPSTEST
                     DLLIEIANKVTSDYFFLQVKTGNISFDDANLIEWLTTTRELGSALSYCHYWQHANDEV
                     TRHNVIEYTEGSIRDDFNVGALVLIKTSLLHEYIKNLQEKYLYAGWYSFRLFLSRKGS
                     IELVNNYAYSTYETDLRASGVKQFDYVNPNNRDRQIEMEKAATHHLQEIGALVKPPFK
                     EVNPDQEIFPAEASVIIPVLNREKTIADAIKSVLAQQTDFTFNLIIVNNHSTDDTARI
                     INTFDDRRIIHHVPQRTDLGIGGCWNEAINHEACGKFAIQLDSDDLYLDETTLQKIIE
                     EFRRQRCAMVIGSYRMVNFNLEEIPPGIIDHREWSDNNGPNNALRINGLGAPRAFFTP
                     IIRQLQFPNVSYGEDYAVAIAISRQWKIGRIYEPIYLCRRWDNNTDSNLSIEKINQHN
                     RYKDSLRTNEIRIRKTMSSKNNE"
     gene            complement(1684..3207)
                     /locus_tag="PKJ43_01220"
     CDS             complement(1684..3207)
                     /locus_tag="PKJ43_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNZ71207.1"
                     /translation="MKITILIIIFSIVTFRAWAEQPKREFRGMWIATVNNLDWPSKAG
                     DKAEKQQEELIAILDTLAKLNFNAVIFQIRPASDAFYRSKTEPWSVYLTGQQGKLPDK
                     NWDPLAFVIRECHKRGMELHAWMNPFRIAQKTSDILSDQNVAMKHPEWVVTYSGRQYL
                     DPGIPDVRQYLKDIVDEVVENYDIDAIHFDDYFYPYPVANAPFPDTLSFKNYGAGYTA
                     ENIDSWRRANVDSIISTLGKSIKSIKPFVKFGISPFGVWRNYDVDVTGSVTQGGASNY
                     DHLFANVTYWQQMGWIDYLIPQIYWEIGHPKADYVTLANWWNEKGFDRHVYIGHALYK
                     LDEGSSEAWKNPRELPEQIAITRTLKNIGGSAFFRMKNLSNNPQQIKTQLEKTLYPSK
                     ALVPPMKWIDNEAPAAPYKVVAHGLFRKKHLTIDYGKNQKQSADHAGFLVYFSTEKNK
                     IDPNNAASLILYTTENQIDIKEIPLPLNKKSYIWVSAVDRLHNESIPVGGVKIKKKK"
     gene            complement(3308..>5277)
                     /locus_tag="PKJ43_01225"
     CDS             complement(3308..>5277)
                     /locus_tag="PKJ43_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ71208.1"
                     /translation="RTIQDLKFKTAVEGQRDKKAIKNGEDIEDQEFRSERNSLLKSIL
                     GFLPWLALSIAPLFSSLIEKIIAWLSIPAKTAKGKVLENAEESDMIASFRRFNPKNII
                     PSAFYRLCIAWDWVNIKYKLIAKHSFKQHWIQFNSIPHDFKHLFYRNETKELLGKNNR
                     NFWLIVAIMFFTFLAIGFSNGSLNYLKNKMEDPFVRSISASIPTTNRQAFRLIDYYNS
                     DSLQQKYRYDTIVGYNKWTAYFQGDLPDSPNKSIDGRSIPHNDPLVATIIDKNINNAV
                     GSAFISENDISIIVTRNFIEKLGCSKEPNFIFRKTRDGRAIPIPIRAIVDKLPGDKID
                     FLATQYFYSNQNSLGDKFLFDDNKKLYAYAVVDSSKIEDFRKASEDFFARYWDEGGML
                     EVNTIDVYPYQFSYKSVIYLQVSFNYSEDIMIEQIDELYTQFRESDEIKKFIDEAKIE
                     EHEFFQAYYPNQENRISRRYDYIAVNFNDLSKVDEFAEKFAEDSEIKLEMAKIEQMKN
                     YNFVSKLTSIMSIILIGFSVLMINIFLSNILSTHLNNIKKNIGTFKAFGIDIKRIYIG
                     MMYIFVLMPLAVAICLAALVGYAGAVYLIIDVLSPFAVEKDLYFDIFNWYTLLSVLVM
                     AIVNYYAFSVIINKIFKQTPGDLIYDRNNKA"
BASE COUNT         1373 a         1052 c         1133 g         1719 t
ORIGIN      
        1 gaaaagcatt gatgcaatgg ctccgtcttc agtaacatat cctaaaataa catggcttgt
       61 atcgccggga atgaagattg cccgcggatc gctaagtccg ggccatgagc cattggtcgt
      121 tagcaccgac catttaattt gcgctgttgc attgtttagc gagaaaattg aaaaaacaat
      181 gatcaggagt attgagagcc gggctgtttt gtatttcaac atgttgcaga aatttttatt
      241 cattattttt agaactcatt gtttttcgaa tacgtatctc gttagttctt aagctgtctt
      301 tgtatcggtt gtgctggttg attttctcaa tactcaggtt ggaatcggta ttattatccc
      361 atcttctgca cagatatatc ggttcataaa tccggccgat tttccactgg cggctgatgg
      421 caatggctac agcataatct tcgccataac ttacattcgg gaattgcagt tggcgtatta
      481 ttggggtgaa aaaggccctt ggtgccccta agccatttat ccgcagggca ttattgggac
      541 cgttgttgtc gctccattcg cggtggtcta taattcccgg gggaatttcc tcaagattga
      601 aatttaccat tcgataacta ccgataacca tggcgcaacg ctgcctgcga aattcttcga
      661 taattttctg taaggttgtt tcatcgagat aaaggtcgtc gctgtcgagc tggatggcaa
      721 actttccgca agcttcgtgg ttgatcgctt cgttccagca accgccaata ccgagatcgg
      781 tccgctgagg cacatggtgt ataattcgcc gatcatcgaa tgtgttgata atcctggctg
      841 tgtcgtcggt cgaatggttg tttacgatga tgaggttgaa ggtaaaatcg gtttgttgag
      901 caagcactga tttaatggca tcggcaattg ttttttcccg gtttaacacg ggaattatta
      961 ccgaagcctc ggctgggaag atttcttggt ctggatttac ttccttgaac gggggcttca
     1021 ccaacgcccc aatttcttgc aaatggtggg ttgctgcttt ttccatctca atttgccggt
     1081 cgcggttgtt cgggttaacg taatcgaact gctttacccc agatgccctc aggtcggttt
     1141 cataagtgga gtaggcatag ttgttgacca gctcgatgct gccctttctt gaaagaaaga
     1201 gcctaaaact gtaccagcct gcgtacaaat atttctcttg cagattttta atgtattcgt
     1261 gcaacaacga tgtttttatc aacactaggg cgccaacatt aaaatcgtct cgtatacttc
     1321 cttcagtata ctctattaca ttgtgtcggg tcacttcatc gttggcatgt tgccaataat
     1381 ggcaatacga tagggcagag cccaattccc gtgttgtggt aagccattca atcaagtttg
     1441 catcatcgaa cgaaatattg cctgttttta cttggagaaa aaaatagtct gaagtgactt
     1501 tgtttgcaat ttcgatcagt aaatctgttg attctgttga tggtgaaaag atatgactaa
     1561 cttcatcagt tgaattatcc ggactaataa cccagccgtt gaagtgggta ttatcgttga
     1621 ataaatgttt gttatacagg aaaatatcgg ttttcattat gcaatgtttt ctggttattt
     1681 tgattacttt ttctttttta ttttcactcc gccaaccgga atactttcat tatgcaggcg
     1741 gtcgactgcg cttacccata tgtaactttt tttattcagc ggtaatggaa tttcttttat
     1801 atcaatttga ttttctgtag tataaagaat tagggaggca gcattgttgg gatctatttt
     1861 attcttttcc gttgaaaaat aaacgagaaa acccgcatgg tctgccgatt gtttttgatt
     1921 tttcccataa tcgattgtta agtgtttttt cctgaaaagt ccatgggcaa caactttata
     1981 tggcgctgca ggcgcttcgt tatcaatcca tttcattggc ggaactaaag ctttgctggg
     2041 gtaaagtgtt ttttcaagct gcgttttaat ttgttgtgga ttattgctca ggttcttcat
     2101 tctgaaaaat gcacttcccc caatattttt caatgtgcgg gttattgcta tttgctctgg
     2161 aagttcgcgg gggtttttcc atgcttcgga gctgccttca tcgagtttat acagggcatg
     2221 gccaatgtac acatgacggt cgaaaccctt ctcgttccac caatttgcca gagtcacgta
     2281 atcggctttg gggtgaccaa tttcccaata aatctgcgga atcagataat cgatccaacc
     2341 catttgttgc caatatgtta cgttggcaaa cagatggtcg taattgctgg ctccgccttg
     2401 tgttaccgag ccggttacgt caacatcgta atttcgccat actccaaatg gactgatgcc
     2461 aaatttcacg aatggcttaa tggatttaat gcttttcccc agggtcgaaa taattgaatc
     2521 gacgttggct cttctccagc tatcgatgtt ttcggcagta tagcctgctc cgtagttttt
     2581 aaacgagagc gtgtcgggga aaggggcatt ggcaacgggg taggggtaga aataatcgtc
     2641 gaaatggatc gcatcgatgt cgtaattttc aacgacttca tctacaatgt ctttaaggta
     2701 ctgccgcaca tcggggattc ccgggtcgag atattgtcga cctgaatagg tcaccaccca
     2761 ttcaggatgt ttcatggcaa cattctgatc actaagtatg tcgcttgttt tctgggcaat
     2821 cctgaagggg ttcatccatg cgtgaagttc cattcctctt ttgtgacatt ccctgatgac
     2881 aaaagccagc ggatcccagt ttttatcggg caatttgcct tgttgtccgg taaggtaaac
     2941 cgaccatggt tctgtttttg aacgatagaa tgcgtcgctt gccgggcgga tttgaaaaat
     3001 taccgcattg aagttgagtt tggccaaggt gtcgagtatt gcaataagtt cttcttgttg
     3061 cttttccgct ttgtctccag ctttcgatgg ccagtcgaga ttgtttaccg tagcaatcca
     3121 catccccctg aattcgcgct ttggttgctc tgcccaagcc ctgaaagtta caatgctgaa
     3181 gattattatt agtatggtaa ttttcatcaa tcgtttttca atttggttaa atgtagaaaa
     3241 gtttacagaa acaacaaagg cagaaaaatt gattcctgcc tttgtttact attctttcaa
     3301 tacattatta cgctttatta tttctgtcgt aaatcaaatc acctggggtt tgtttaaata
     3361 ttttattgat aatcacactg aatgcatagt agttcactat tgccattacc aataccgaaa
     3421 gcagcgtgta ccagttgaag atatcgaaat aaagatcttt ttcaacggca aacggcgaga
     3481 ggacatcgat aatcaaatac acggctccgg cataaccaac cagtgctgca aggcaaatag
     3541 ccactgcaag gggcataagt acaaaaatgt acatcatacc aatgtagatt cgttttatat
     3601 cgataccaaa tgctttaaat gttccgatgt tctttttgat attgttcaag tgcgtgctca
     3661 ggatatttga aaggaaaata ttgatcatca gaacactaaa accaatgaga atgatcgaca
     3721 tgatgctggt cagcttgctt acaaagttgt agtttttcat ttgttcaatt tttgccattt
     3781 cgagctttat ctctgagtct tcggcaaatt tctcagcaaa ttcatcaact ttgcttaagt
     3841 cgttaaaatt tacagcaatg tagtcgtatc gtcgtgaaat acggttttcc tggttcggat
     3901 aataggcctg gaagaattca tgctcttcaa ttttggcctc atcgataaat ttctttatct
     3961 cgtctgattc ccggaattgg gtatacaatt catcaatttg ttcaatcatt atatcttcag
     4021 aataattgaa ggaaacttga aggtaaataa ctgatttgta tgagaattga tatgggtata
     4081 catcaatggt gttcacttcg agcatgcctc cctcgtccca atagcgggca aagaaatctt
     4141 cactggcttt tctgaaatct tcaattttcg acgaatcgac tactgcataa gcatacagtt
     4201 ttttattgtc atcgaaaaga aatttgtcgc ctaacgagtt ttggttacta tagaaatatt
     4261 gtgtggccaa aaaatcgatt ttatctcccg gcaatttgtc aacaatagcc ctgattggaa
     4321 taggaatagc ccttccatcc ctggtttttc tgaaaatgaa gtttggttct ttggagcagc
     4381 caagtttttc aataaagtta cgggttacta taatcgaaat gtcattttca gaaatgaacg
     4441 cactccccac ggcattgtta atatttttgt cgataatggt agctaccagc gggtcattat
     4501 gcgggataga acgtccgtca atacttttgt tcgggctatc gggaaggtcg ccctggaaat
     4561 acgctgtcca tttgttatac ccaacaatgg tgtcgtagcg gtatttttgt tgcaacgagt
     4621 cagagttgta ataatcgatc agccggaagg cctgccggtt tgtggtaggt atcgacgcac
     4681 ttattgaacg cacaaacggg tcttccattt tgttcttcaa atagttcaaa ctgccgtttg
     4741 aaaagccgat agccaggaag gtgaagaaca taatggcgac aataagccag aagttccggt
     4801 tgtttttacc cagcagttct ttcgtttcgt tcctgtaaaa aaggtgtttg aaatcgtggg
     4861 gaatggaatt aaactgaatc caatgctgtt taaaactgtg tttggcaata agcttatatt
     4921 ttatatttac ccagtcccag gcaatacaaa gcctgtaaaa tgccgatggg ataatatttt
     4981 ttgggttgaa gcgccggaat gaagcaatca tgtcactttc ttcagcgttt tcaagtactt
     5041 tgcccttagc cgttttagca ggtatagaaa gccatgcaat aatcttttcg ataagtgagc
     5101 tgaacagcgg ggcaatagat aaagcgagcc atggcaaaaa accgagaata cttttcagta
     5161 aggagtttct ttcacttctg aattcctgat cctcaatgtc ttcaccgttt tttattgctt
     5221 tcttgtcacg ctggccttca acggcagtct tgaattttaa atcctgaatg gtacgga
//
LOCUS       DAOPUW010000038         5268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_15693_length_5268_cov_3.118358, whole genome shotgun sequence.
ACCESSION   DAOPUW010000038 DAOPUW010000000
VERSION     DAOPUW010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15693_length_5268_cov_3.118358"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1699
                     /locus_tag="PKJ43_01230"
     CDS             <1..1699
                     /locus_tag="PKJ43_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNZ71209.1"
                     /translation="EDTPDQLKATQAVKEDMERLMPMDRLVCGDVGFGKTEVAIRAAF
                     KAATDSKQVAVLVPTTILAFQHYKTFSERLKDFPVKIGYISRLRNSADTAKTLKELSE
                     GKIDIVIGTHRLVGKDVKFKDLGLLIVDEEQKFGVSVKEKLKQLKINVDTLTLTATPI
                     PRTLQFSLMGARDLSIIQTPPPNRYPIVTEIHGFNEAIIREAINYEVDRGGQVFFIHN
                     RVQNIHEIEAFVRRACPDVKTIVGHGQMDGEKLEKIMLDFINGEYDVLIATTIIESGL
                     DIPNANTIIINHAQSFGLSDLHQLRGRVGRSNKKAFCYLLSPPMNTLTQEAQRRLRAI
                     EEFSELGSGFNIAMRDLDIRGAGNLLGGEQSGFIADIGFETYHRILDEAVQELKQEEF
                     RDVFAQRDSDEAAKAFLNTRFVTDCNVETDMEMRLPESYVNSISERMSLYRELDNIEN
                     EEELSRFENMLTDRFGKIPEPAQQLLQVVRLRWIAIELGMERIILKNGKMICHFIANQ
                     QSLYYQSPTFSAILSWLQRNPEKCKMKEEKDKLSMSFEKVKSVSQAYQILQNINEKH"
     gene            complement(1812..4799)
                     /locus_tag="PKJ43_01235"
     CDS             complement(1812..4799)
                     /locus_tag="PKJ43_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNZ71210.1"
                     /translation="MVDINTVSLSSILEKRKNDRDIFQELMPSKVKEILLVATRYDAY
                     SIVREGQFSDKIFGEYLQLILYSAPRFTSVSTLSEAVDVLRSKHFDLIIIMAGVDRQS
                     PILTAEKLFEVKPRIPILLLVNNNYDLSFFTKAAEKLDCIDRVFVWNGNTTIFLAMIK
                     YIEDKKNVARDIQVENVRVILLVEDSVKYYSRYLPMLYTNIMLQTQNLVSDDSTDELH
                     MILKMRARPKILLVSNFEEAIALVDRYKDNLLCVISDVKFAKEGDDNHDSGIELLNYV
                     KANLRFPIPLLLQSHDVHNQISAEQIGADFINKNSDTLSKDILSFIHRRLGFGNFEFF
                     MPDGTKIAEASTLHEFQELMKKIPIESLIFHGRENAFSTWLMARGEINMAERLKVTQV
                     DEFEQKQDLREFILKVFDKIRMQQLSGRIVNFDPALVAGNRYIIRLSKGSLGGKGRGL
                     AFISNFIESIDFKKIIPKINIRIPPTAIIGVHEFDAFIESNDLYNKVIVEQHYDSVNE
                     LFIKGKISEQLNERLFQYLVTMRNPLAVRSSGLFEDSLLQPFAGVYNTYILPNNSPDI
                     NIRLEQLKDAIKLVYASTFAATARNYFNAVNYKVEEEKMAVIIQEAVGEHFGNLFFPH
                     ISGVAQSFNYYPVSYMEPSDGYSVACVGFGMYVVGGEKSFRFCPRYPSINPGGVKDLL
                     RDTQTELYALNLEHSHFDLKNEGELAAIKKLKVKEIDKNGAMSFCLSTYDYENDCLVP
                     GVALKGPRVVDFANILKYSSIPLADSLHLLLRIFREAMGAPVEMEYAVDLSPAPENGL
                     PTLYLLQIKPLIRREEANEVVIDKGDPNDSLLYATRGMGNGVIDHLRDVVYVDVAGFD
                     RTRTKEIAAELAQFNEMLVNEGKEYILIGPGRWGTRDPFTGIPVTWSQISNARVIVEM
                     GLEDFPLDASLGSHFFHNVTSMNVGYFSVPFNSNEAYINIEELKKFPVVRRTNFLRHV
                     RFPRPVSVLMDGKQRVALIKLPD"
     gene            5053..>5268
                     /locus_tag="PKJ43_01240"
     CDS             5053..>5268
                     /locus_tag="PKJ43_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71211.1"
                     /translation="MIRKITGLILILGLLTACNETSETIHSHANKAYTKAIADSLAFR
                     IEIPGDEIQLKAKIKIIGAKSIPPKTVG"
BASE COUNT         1695 a         1176 c         1052 g         1345 t
ORIGIN      
        1 cgaagatact cctgaccaac taaaagccac acaagctgtt aaagaagata tggaacgact
       61 gatgcccatg gaccggttgg tttgtggtga cgtaggtttt ggcaaaaccg aagtggccat
      121 tcgtgcagcc tttaaagcag cgaccgacag taaacaagtt gcagtactag tacccaccac
      181 catattggca tttcaacatt acaaaacatt ttccgaaaga ctaaaagact ttccggtaaa
      241 aattggatac ataagccgct tacgcaacag tgccgacacc gctaaaaccc tgaaagaact
      301 ttccgaaggg aaaattgaca tagtgattgg tacccaccgg ctggttggca aggatgttaa
      361 attcaaagat ctgggattgc taattgttga cgaagaacag aaatttggcg tctcggtaaa
      421 agaaaagctg aaacaactga aaattaatgt cgatacgctg acccttacgg ctacgccgat
      481 accccgtaca ttgcaattct cgcttatggg cgcgcgcgac ctttctataa ttcaaacccc
      541 gccaccaaac cgttatccca ttgtgacaga aatacatgga tttaacgaag caatcattcg
      601 cgaagccata aactatgaag ttgaccgagg cggacaagtc tttttcatcc ataacagggt
      661 acagaatatt cacgaaattg aagcgtttgt gcgccgtgca tgccccgatg tgaagaccat
      721 tgttggacat ggccagatgg acggtgaaaa actggaaaaa ataatgctcg atttcatcaa
      781 tggcgaatac gatgtactga tagccacaac aatcattgaa tcgggactcg acattccgaa
      841 tgccaatacc attataatta accatgcaca aagctttgga ctgagtgatc ttcaccagct
      901 gcgagggcga gtaggccgct cgaacaagaa agcattctgc tatctgctgt cccccccaat
      961 gaacaccctc acacaggaag cccaacgaag actaagggcc atagaagaat tttcggaact
     1021 gggaagcggg ttcaatattg ccatgcgcga ccttgacata cgcggagccg gaaacttgct
     1081 gggaggtgaa caaagcgggt ttattgccga cataggtttc gaaacctatc accgcattct
     1141 cgacgaggct gttcaagagc tcaaacaaga agaattcagg gatgtttttg cccaacgtga
     1201 ttccgacgaa gctgctaagg catttttaaa tacccgtttt gtaaccgact gtaatgtaga
     1261 aaccgacatg gaaatgcgcc tgcccgaaag ctatgtaaac agcatttcgg aacggatgtc
     1321 gttataccgg gaactggata atattgaaaa tgaagaagaa ctgtcgcgct ttgagaacat
     1381 gctcaccgac cgttttggaa aaatacccga acccgcacaa cagttgctcc aagtggttcg
     1441 gctacggtgg atagcgattg agttgggcat ggagcgtatt atcctgaaaa acgggaaaat
     1501 gatatgccac ttcattgcca atcaacaatc gctatattac caatcgccca ccttttcggc
     1561 aatattgtcg tggctacagc gcaaccctga aaagtgcaaa atgaaagaag aaaaagacaa
     1621 gctcagcatg tcattcgaaa aagttaaaag tgtttcgcag gcttatcaga ttctccaaaa
     1681 tataaatgaa aaacattaac aacccacaaa tattcaacgc acaacaaatt atgtctttaa
     1741 aggaataaag ccatgagatg gcaaacgata aagtcaggtg cagggaaaaa gcattcaaaa
     1801 attagcaaca actaatcagg caatttaatc aaagctaccc gctgctttcc atccataagc
     1861 actgaaaccg gacgcggaaa gcgcacatgg cgcagaaaat tggtacgacg caccacggga
     1921 aatttcttta gctcttcaat attaatataa gcttcattag aattaaaagg aacagagaaa
     1981 tagcccacat tcattgacgt tacattgtga aaaaaatgag accccaacga agcatcaagg
     2041 ggaaaatctt cgagccccat ttcaacaata acacgggcat ttgaaatttg cgaccatgtt
     2101 accggtatcc cagtaaacgg gtcgcgagtg ccccaacgcc ctggtccaat caagatatac
     2161 tctttgccct cgttaaccag catttcgttg aactgtgcca attctgcagc aatttctttt
     2221 gtgcgtgtcc ggtcgaagcc agcgacatca acatacacca catcgcgcaa atggtcaata
     2281 accccgttcc ccataccccg ggtagcatat aggagcgagt cgttagggtc acccttgtca
     2341 atgaccacct cgttagcttc ctcacgccgt ataagcggct ttatctgaag cagatacaat
     2401 gttggcaacc cattttcggg tgcaggggaa aggtctactg catattccat ttctaccggc
     2461 gctcccatag cttcgcgaaa aatacgcaat aatagatgga gtgaatcggc caaaggaatc
     2521 gaactatatt tcagtatgtt ggcaaaatct actacccttg gcccctttaa agctactccc
     2581 ggaactaaac aatcattctc gtagtcatag gtcgaaaggc aaaagctcat cgctccattt
     2641 ttgtctattt ctttaacctt caacttcttt attgcagcca actcaccttc attttttaga
     2701 tcaaaatgag aatgttccaa attgagggca tacaactcgg tttgagtatc gcgcaaaaga
     2761 tcctttaccc cgcccggatt gattgaaggg taacgcggac aaaagcgaaa ggatttctcg
     2821 cccccaacca catacatacc aaagcccaca caagctaccg aatatccgtc agacggctcc
     2881 atatacgaaa ccggataata gttaaacgac tgtgcaactc cactaatatg aggaaaaaac
     2941 aaatttccaa aatgctcacc tactgcttct tgaataatta cagccatttt ttcttcttct
     3001 accttgtagt tcacggcatt gaaatagtta cgtgctgtgg cagcaaaagt agaggcataa
     3061 accaatttga tggcatcttt taattgttcg agacgaatgt ttatatcagg actgttgttg
     3121 ggcaaaatat aagtattata aacccccgcg aaaggctgca gcaacgaatc ctcaaaaaga
     3181 cctgatgacc tgactgctag tggattacgc atagtcacca aatactgaaa caagcgttcg
     3241 ttgagctgct cacttatttt cccttttata aacaattcgt ttactgaatc atagtgttgc
     3301 tcaacaatta ctttattgta caaatcgtta ctttcgataa acgcgtcgaa ttcgtgtacg
     3361 ccaatgatgg ctgttggcgg gatacgaata tttattttag gtataatttt cttaaaatca
     3421 atactttcaa taaaattaga aataaaagcc aatccacgtc ctttacctcc taacgagcct
     3481 ttactaagcc gaataatgta ccggttaccc gcaacaagtg ccgggtcaaa atttacaatg
     3541 cgtccactga gttgctgcat tctgattttg tcgaagacct taaggatgaa ctctctcaag
     3601 tcttgcttct gttcgaactc atcaacctgg gttaccttga gcctttcggc catattaatt
     3661 tcaccacgcg ccataagcca tgttgaaaaa gcattttccc gtccatgaaa gatgagcgac
     3721 tcgataggga tttttttcat caactcctga aattcatgca acgtagaagc ttcagcgatt
     3781 ttcgtcccat cgggcataaa aaattcaaaa ttgccaaacc caagcctgcg atggataaaa
     3841 cttaatatgt cttttgaaag agtatcggag tttttattaa tgaaatcggc accaatttgt
     3901 tcagcactaa tctgattgtg aacatcgtgc gattgcagga gcaaaggaat cgggaagcga
     3961 aggtttgctt tgacatagtt aagtaattcg atacccgagt cgtgattatc gtcgccttct
     4021 ttggcaaatt tcacatcgct gattacgcaa agcagattgt ctttataccg atctaccaat
     4081 gcaattgctt cctcgaaatt actcaccaac aatattttag gacgggcacg catttttagt
     4141 atcatgtgaa gctcgtcggt cgaatcgtcg gaaacaaggt tttgagtctg caacatgatg
     4201 ttggtataaa gcattggcaa atagcgcgaa tagtatttca ccgagtcttc aaccagcaat
     4261 atcacccgta cattttccac ttgaatatcc cgtgccacat tcttcttgtc ttcaatgtat
     4321 tttatcatgg ccaggaaaat ggttgtgttg ccattccaca caaaaacgcg atcgatacag
     4381 tcgagttttt cagccgcctt ggtaaaaaat gaaagatcgt aattgttgtt gacaagcaat
     4441 aatataggaa tacggggttt tacttcgaag agtttttctg cggtaagaat cggcgattgg
     4501 cgatcgacgc cagccatgat tattataaga tcgaaatgct ttgaccgcaa cacatctact
     4561 gcttcagaga gcgttgacac actggtaaaa cgcggagccg aataaagaat aagctgcagg
     4621 tattcgccaa atatcttgtc agaaaattgc ccttcgcgaa caatagagta agcatcgtag
     4681 cgagtggcaa ccaacaaaat ttccttcact tttgaaggca tcaattcctg aaaaatatcg
     4741 cggtcgtttt tccttttttc aagaattgac gacaaggaaa cggtattaat atctaccata
     4801 aaaatgcagc ataaaacata gtcaaacaaa attaggaaaa agaggtcgcc caaatcacga
     4861 aaagggaaat aaaaaacaaa aaaacgtacg gctggtgaaa aataaaacga atcaaaagtg
     4921 tgaaactatg ctaaagggag ccattggtaa cgcaagtaaa tcgtttaaat ttgtattttt
     4981 acggcccggc ttcaaagttt ttttagtatt atctttaaga tcaattcatt cagtgaacag
     5041 taaaaagaaa ccatgatacg caaaataacc ggtttgatac tcattcttgg cctacttacc
     5101 gcttgtaacg aaacaagcga aacaatacac agtcatgcta acaaagctta caccaaagca
     5161 atagccgatt cgcttgcttt caggatagaa attcccggag atgaaatcca actaaaagca
     5221 aaaattaaaa taataggcgc taaatccatt ccaccaaaaa ctgttgga
//
LOCUS       DAOPUW010000039         5263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_15718_length_5263_cov_3.555684, whole genome shotgun sequence.
ACCESSION   DAOPUW010000039 DAOPUW010000000
VERSION     DAOPUW010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15718_length_5263_cov_3.555684"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(705..3656)
                     /locus_tag="PKJ43_01245"
     CDS             complement(705..3656)
                     /locus_tag="PKJ43_01245"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HNZ71212.1"
                     /translation="MTTNTKENGFETLIVDYLVNQNQYELGNNTDYNKEFAIDETRLF
                     RFLQETQAEKIEALHILDSELQRSKFLSKLKQELGNRGVIDVIRKGMRYLHLILDFYY
                     VTPSAQNPKAKELYNQNIYSVTRQVKYSKEFPNLALDFVIFINGLPIATFELKNQFTK
                     QDVYDAVHQYKTDRDPKETIFNFKKCAVHFALDDNEIRMCTRLAGKSSWFLPFNKGRN
                     KGAGNPDNKNGIKTDYLWKEILTKSELSYIIENFAQVVSEKDEDTGRIKETQIFPRFH
                     QLSVVKALLTDTQNKGAGQRYLIQHSAGSGKSNSIAWLAHQLVVLENDGKNIFDSIIV
                     VTDRINLDKQIRNTIRNFMQESSTVGWAEKSSVLKDLLASGKKIIITTIFKFPEILKD
                     ISTENKDKNFAIIIDEAHSSQNGSLSATMNIALSGSISKDDDDLEDKINKIIKGRKML
                     KNASYYAFTATPKNKTLEMFGVPVQHADGTIGHEPFHEYTMKQAIEEGFIMDVLKFYT
                     PIISNYLLSKKINDDPNFDRKKAKKRLKAFVEGNQYTIEQKAGIIVEHFHNQVISKGK
                     IGGQARAMVITASITRAIDYYYAITKLLEERNSQYKAIVAFSGEKEHKVYGKVTESSI
                     NGFPNNMIEKTFKKEPYRFLIVADKFQTGYDEPLLHTMYVDKVLADIKAVQTLSRLNR
                     AHPLKKDTFVLDFANDVEVIRESFQRYYKTTILSSETDPNRLNDLISSMERHQVYTQY
                     HIDEVVRLLLGNENRDRIDPLIDQCVTNYKELGEDDQVEFKSSAKAFERSYTFLAAIM
                     PYESPEWEKLSMFLHLLVAKLPSPIEEDLSEGILEAIDFDSYKNNILEEQAIKLENED
                     AEIEPVPLGTISTVNEPDLDTLSNIINEFNTRFGDIQWSDSDKIKKQIAELPNEVAKS
                     EAYQNAMKNSDKATAKIESDAALMKAVLQSMTSGVELFKQFQDNPSFKKWLQEFVFMN
                     TFNNPHQPIRR"
     gene            complement(3855..>5263)
                     /locus_tag="PKJ43_01250"
     CDS             complement(3855..>5263)
                     /locus_tag="PKJ43_01250"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HNZ71213.1"
                     /translation="ENSRIERTISLTNTDKFGEAICAFSNDFPDTKQAGYLIIGANDN
                     GSRSGLKVTDQLQINIAAIRSDGNIQPQPAMSIEKFTFPDGELLVAEVQPSNLPPVRY
                     KSKIWIRVGARKAVANEAEEKILYEKRAANIKTFDAMPCTGATIDDLDVKLFKTEYLP
                     KAFPEDILMEDKRDIKQKLQSLGFFDLRFDCPTYAGIVLFGVKPEKFLAGCYVQYVRF
                     KGEDRAGEITNEHKFDGNLVRALQKMDTFVELSIAKEKPVPVSALREMKVISYPYYAT
                     RELLMNAVMHRDYESNAPTRFYEFDSYIEIQNAGGLYGKARPENFPDVNDYRNPIIAE
                     AMKVLGYVNRYNRGIIRVQKELEENGNGKATFDLNLVTAFKVVEPISVRANSELDELT
                     EKQLKILEYCIEAKSREDILSWVGLSNQTKNFNKHVVPLIEAQLLAFTKSEKPNVRGQ
                     QYILTEFGRTIFNAEENE"
BASE COUNT         1386 a         1089 c          978 g         1810 t
ORIGIN      
        1 cagcccaggg caaagtgaaa cgccgcccta ggtaaaaatg ggaaacaaaa caaaccgtcc
       61 gcgaacaaat gttgggtatt cataaaccgt tatttcggac ggaatagccc ctcaattgta
      121 ttttgcgttt ataaacatga caaggcaatt aacccgtatt gcatccgaag aaacatttcc
      181 acctcgtcga acatcctacc gcggacgctt atggtttttg ccatttaacc cagggcgacc
      241 ctccttcgtc agttgccctg ggctgaatta atttgggcct tcagcccgtt tgtatcgttt
      301 cgaatgacag tgtctaccac gtatgtttca aacaactgat cctatatgat ttttatctca
      361 ccaacttttc cggttgatcc cccgctactt cagagagaat caaaaaaatt ggttctcttt
      421 ttctcattcc tatttgatat tcgttgtact tttacctatg caaattttaa tatcattgca
      481 gacctggttg ctccaggtat aggctctttg aaatttgtat taaacagtta atggcagctt
      541 cggactgttt ccgttgtgat gtattaactg tttttttgtt accgatctca tcttctaaat
      601 tattaagggt caaacggaat ttctgggagg caattactgt cgggttcatc ggggcattgc
      661 ttcacctatt tcgctttcac aaaatgaaca atcccgcaaa tatcttaacg tcttatgggt
      721 tgatgtggat tattaaaagt gttcataaat acaaattcct gcaaccactt tttaaatgat
      781 ggattgtcct ggaattgctt aaacaattcg actcccgatg tcattgattg taatactgct
      841 ttcattaatg ctgcatcact ttcaatttta gctgttgctt tatctgagtt tttcatggca
      901 ttctgatatg cttcgctctt agctacctca ttgggtaatt ctgcaatctg cttctttatt
      961 ttatctgagt cgctccattg aatgtctccg aaccgtgtat tgaattcatt gataatattg
     1021 ctcaatgtat ctaaatcagg ctcgtttact gttgaaattg ttccaagggg aactggttct
     1081 atttccgcat cttcgttttc aagtttgatc gcttgttctt ccaaaatgtt attcttgtaa
     1141 ctatcaaaat caatagcttc cagaattcct tccgacaaat cctcttcgat tggtgaaggt
     1201 agttttgcaa ctaacagatg aaggaacatg gatagttttt cccattctgg actttcgtag
     1261 ggcataatag cagccaaaaa tgtataggat cgttcgaaag ctttggcact gcttttaaac
     1321 tcaacctgat cgtcttcacc gagttcttta taattcgtta cacattgatc gatcagagga
     1381 tcgattcgat ctcgattttc attaccgagc aacaagcgaa caacttcatc gatatgatat
     1441 tgtgtatata cctgatgcct ttccatcgac gaaatcagat cgttcaatcg gttaggatca
     1501 gtttccgatg aaagtatggt ggttttgtag taacgttgaa atgattccct gattacctca
     1561 acatcattgg caaaatcgag tacaaatgta tctttcttca aagggtgcgc acggttcaaa
     1621 cgagatagcg tttgtacggc tttaatatca gccaatacct tgtcaacata catggtatgc
     1681 aataaaggtt cgtcataacc tgtttgaaat ttatctgcaa caattaaaaa tcggtatggc
     1741 tctttcttga atgttttttc aatcatattg ttcggaaagc cattgattga cgattctgtt
     1801 acttttccat atactttatg ttccttctcg cctgaaaagg ccacaattgc tttgtattga
     1861 ctattccgtt cttccagcag ttttgtaatg gcataataat aatcaatggc tcgtgtaatg
     1921 ctggcagtta ttaccatagc tcttgcttgc ccgccaattt tacctttact tattacctga
     1981 ttatgaaagt gttcgacaat tatcccagcc ttttgttcga tcgtgtattg atttccttca
     2041 acaaaagctt ttagccgttt ctttgccttt tttcggtcaa aattgggatc gtcattaatc
     2101 tttttcgaca atagataatt actgattatc ggggtgtaaa atttcagaac atccataatg
     2161 aaaccttctt caatggcttg tttcatcgtg tattcatgaa atggctcatg cccgattgtt
     2221 ccatcggcat gttggacggg cacaccaaac atttcaagtg ttttattctt tggcgtagcc
     2281 gtaaatgcgt aataactggc atttttaagc attttacgcc ctttaataat cttgttgatt
     2341 ttatcttcca gatcatcatc atctttgctt atgctgcctg acaaggcaat attcattgtg
     2401 gccgataaac ttccattttg gcttgagtgt gcttcgtcga tgatgattgc aaagttctta
     2461 tccttgtttt cggttgatat atcttttagt atttcaggaa acttgaagat ggtggtaatg
     2521 atgattttct ttcccgaagc caataaatct tttaaaacac tgcttttttc agcccagcca
     2581 accgtactgc tttcctgcat gaaatttcgg atggtgttcc gaatctgttt atcaaggttg
     2641 attcggtcgg taacgactat gatagaatcg aaaatgtttt taccatcgtt ttccaacaca
     2701 accaactgat gtgccaacca tgctattgaa ttcgattttc cactacctgc actgtgctgg
     2761 ataaggtatc gttgtccagc tcccttgttt tgcgtatcgg tcaataaagc tttaacaacc
     2821 gacagttggt gaaaccgtgg gaatatctgt gtttctttta ttctacctgt atcttcatcc
     2881 ttttcgctga caacctgtgc aaaattttca ataatgtacg agagctccga ttttgtcaat
     2941 atctctttcc acaggtaatc ggttttaatt ccatttttat tgtcagggtt tcctgccccc
     3001 ttgttccttc ctttgttgaa aggtaaaaac cacgatgatt tgcccgccaa cctggtacac
     3061 atccgaattt cattgtcgtc taatgcaaaa tgaaccgcac actttttgaa gttgaaaatt
     3121 gtttctttgg gatcacgatc ggttttgtat tgatgcaccg catcgtacac atcctgtttg
     3181 gtgaactggt tttttaattc gaatgtggct attggcaaac cgttaatgaa aatgacaaaa
     3241 tcgagtgcca aattcgggaa ttcttttgaa tactttacct gacgggtaac actgtaaata
     3301 ttttgattgt aaagttcttt tgctttgggg ttttgtgccg acggtgtaac ataatagaaa
     3361 tcgagaatca gatggagata acgcataccc ttacggataa catcaataac acccctgtta
     3421 cccaattctt gtttcagctt actgagaaac ttacttctct gcaattctga atccagaatg
     3481 tgcaaagctt ctattttttc ggcttgagtt tcctgtaaaa atcgaaacaa tcgggtttca
     3541 tcaatggcaa attccttgtt gtaatcggta ttgtttccta attcatactg attttggttt
     3601 accagataat cgacgatcaa cgtttcaaat ccattttctt tcgtgttggt tgtcatattt
     3661 ttcggtttct gaggttcggc aaaagtaact agcaagtaac tagcaagtaa ctagcaaaat
     3721 tttcttttaa aattgaatga tcttttaaat atatctgaaa tactgatggt tgaatgattt
     3781 tttcatcatt cgatagctca ctgaattgat cgaaaaagta actagcaaat agtaaaaagt
     3841 aactagcaaa tggcttattc attttcctcg gcattaaaaa ttgttcttcc aaattcagta
     3901 agaatatatt gttggcctct aacatttggt ttttctgatt tggtaaaggc taacaattga
     3961 gcttcgatta atggaacaac atgcttgtta aagtttttcg tttgatttga tagccccacc
     4021 caagaaagga tatcttcccg acttttggct tcaatgcaat attccagaat cttcaattgc
     4081 ttttcggtca attcatctaa ttctgaatta gctcttactg aaatgggttc aaccacttta
     4141 aaagcggtga ctaaattcaa atcgaaggtt gctttcccgt tcccattttc ttccagttcc
     4201 ttttgaactc ggataattcc cctgttgtag cggttaacat acccaagtac tttcattgct
     4261 tcggcaatga tgggattacg gtaatcgttc acatcgggga aattttcagg tcgtgctttg
     4321 ccatataagc cacctgcatt ctgtatttca atgtagctgt cgaactcgta gaaccgtgtt
     4381 ggtgcattcg attcatagtc gcggtgcatc actgcattca tcaacaattc acgagtagca
     4441 taatatggat aactaatcac tttcatttcg cgtaatgccg acacgggtac aggtttttct
     4501 ttggcaatgc ttagttcaac aaaggtgtcc attttttgaa gcgcccgcac caggttacca
     4561 tcaaatttgt gttcgttcgt gatttcgcct gcacggtctt cgcctttaaa acgaacatat
     4621 tgcacgtagc accccgccaa aaatttttcg ggtttcaccc caaacaggac aatgcccgca
     4681 tacgttggac aatcgaaacg caaatcgaaa aatcccaacg attgcagttt ttgtttaatg
     4741 tccctcttgt cctccattaa aatatcttcg gggaaagcct tgggcagata ttctgtttta
     4801 aacagtttaa catccaaatc atcgatggtt gcacccgtgc agggcatggc atcgaaggtt
     4861 ttgatgttgg ctgcccgttt ttcgtagaga atcttttctt cggcttcgtt ggcaactgct
     4921 tttcgggcac ccacacgaat ccagatttta cttttatacc taacgggcgg taaattggat
     4981 ggttgaacct cggcaaccaa caactcgcca tcaggaaacg taaatttttc gatggacatg
     5041 gcgggttggg gttgaatatt cccatcggaa cgaatggcgg caatgtttat ttgtaactgg
     5101 tcggttactt tcaacccgct acgggaaccg ttatcgtttg caccgataat caggtagcct
     5161 gcttgtttgg tatcaggaaa atcgttgcta aacgcacaaa ttgcttcacc aaatttatcg
     5221 gtattggtaa gcgaaatggt acgttcaatc cgtgaattct cta
//
LOCUS       DAOPUW010000040         5245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_15790_length_5245_cov_3.384971, whole genome shotgun sequence.
ACCESSION   DAOPUW010000040 DAOPUW010000000
VERSION     DAOPUW010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15790_length_5245_cov_3.384971"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PKJ43_01255"
     CDS             complement(<1..258)
                     /locus_tag="PKJ43_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71214.1"
                     /translation="MESPGDHIESLIEKGEQYGKTTLELVKLKTIDKGAGALSELISW
                     GIVIIFAVVFFTLANIALALWIGSLLQTMYLGFVIVASFYGL"
     gene            complement(279..524)
                     /locus_tag="PKJ43_01260"
     CDS             complement(279..524)
                     /locus_tag="PKJ43_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNZ71215.1"
                     /translation="MSSDNGKIVLGVLAGLAAGALLGILFAPDKGSETRKKIINKGEE
                     ASDGLKEKLNDLLNDLNQKIDAAKAKARETTSETSNS"
     gene            711..1901
                     /gene="kbl"
                     /locus_tag="PKJ43_01265"
     CDS             711..1901
                     /gene="kbl"
                     /locus_tag="PKJ43_01265"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNZ71216.1"
                     /translation="MYGELKNQLVNSLNSLRENGLYKEERIISSEQGAVINVDGVGEV
                     LNFCSNNYLGLASNREVVEAAKMAIDRWGFGLSSVRFICGTQQIHKELERAVSDFLGT
                     EDTILYCAAFDANGGVFEPLFDENCAIISDELNHASIIDGVRLCKAKRLRYRHSDMEN
                     LEECLKEAKETTYKIIVTDGVFSMDGDIARLDIICSLAERYGALVMVDDSHATGYIGP
                     TGKGTAELFGLTGKIDIITSTFGKALGGGNGGFTSGRREIIEYLRQRSRPYLFSNTLA
                     PATVGASLKVLEMLENSTSLCDAVTRNALYFRQKIEQAGFTVNKGETAIVPVMIFDAP
                     MAVRFADLLLNEGIYVIGFVYPVVPQGKARIRVQLSAAHTLSQIDKAVDAFVKIGKQL
                     HVIN"
     gene            2034..2261
                     /locus_tag="PKJ43_01270"
     CDS             2034..2261
                     /locus_tag="PKJ43_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71217.1"
                     /translation="MGAIERKLYKVLRNVGVRKNYISKAKSIDDLFLDEFDRKLLRFY
                     FENEFKVRLSERDELKLTTLNEVNGFLRCTK"
     gene            2392..3714
                     /locus_tag="PKJ43_01275"
     CDS             2392..3714
                     /locus_tag="PKJ43_01275"
                     /inference="COORDINATES: protein motif:HMM:NF017396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HNZ71218.1"
                     /translation="MNYTKLFRINIALVLGVLCLQIHVFAQNTDGLINQLKEFPEVVS
                     VQKIENNPFFKESYEIYIEQFLNHKNPSEGKFRQRVILSDYNRYSPVVFVAEGYDAEY
                     AMKPSYINELSKILEANQLVVEHRFFGKSVPEGKNWGYLTMQSASDDLHRIQRIFKRL
                     YNNQNKWIVTGISKGGTNTLAYKAFYPDDADIWVPYVGPVNFAVEDGRHEKFLKAVGS
                     QTCREKLLQFQRSILTQREAIQPLFDSLVKARNYSFRIPPEQVLDYCVLEYSFAFWQW
                     GTNCAAIPADSTPIRVQLNHLVEVCSPDYFSNEGTAPMQSFFIQAAKEMGYYGYDVKA
                     LKPLLKIKDAEGYLSEIFIPEGLAFKFDKKTSKFIEKTVNANGKHVLMIYGETDPWSA
                     SGFNLKNPKSGAVKMVIPKASHRVRIENMPLAEKAQVYMLLESWLNED"
     gene            complement(3711..4268)
                     /locus_tag="PKJ43_01280"
                     /pseudo
     CDS             complement(3711..4268)
                     /locus_tag="PKJ43_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
     gene            complement(4214..5092)
                     /locus_tag="PKJ43_01285"
     CDS             complement(4214..5092)
                     /locus_tag="PKJ43_01285"
                     /inference="COORDINATES: protein motif:HMM:NF017046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HNZ71219.1"
                     /translation="MFDKQLYITRRRILKEKIKQGVILFFGNDESPMNYPDNPYHFRQ
                     HSSFLYYFGIDRPSLAAIIDVENDEEIVFGDELTIDDIIWMGNVPTLSEECQKVGINS
                     VLPIVKLYTILREAQASDRNIHFLPPFRPENKIKLLRLLNIRPDQFNQKSSEDLVKVV
                     ISQREIKDEFEIAEIDKAVNWTVDMHEKAISMARPGMFEAEIAAAVTQIPLERQGYIS
                     FPVIASINGAVLHNHYHGNQLKEGQLFLLDAGAEHHRAMPETSQVPFRYRKNSATNSA
                     KFIKLCSMPTTVLLHA"
BASE COUNT         1470 a          988 c         1139 g         1648 t
ORIGIN      
        1 caacccgtaa aatgaagcta caataacgaa gcccaaatac atggtttgca gcaacgaacc
       61 aatccataag gccaacgcaa tattggccag ggtgaaaaaa acaacggcga aaatgatgac
      121 aataccccac gatataagct cggataaagc cccagcccct ttgtcgatcg tttttaattt
      181 tactagttca agtgtggttt tgccgtattg ttcccctttt tcaatcagcg attctatatg
      241 atctcctgga ctttccatga ttaatgaatg tcaattgctt atgaattaga ggtttctgat
      301 gtggtttcgc gggcttttgc ttttgctgcg tcaattttct gattcaaatc gttgagcagg
      361 tcgttgagtt tttctttcaa tccatcactg gcttcctcgc ccttgttgat aatcttttta
      421 cgggtttccg aacctttatc aggggcaaag aggattccca gaagtgcgcc tgctgccaat
      481 cctgctagca ctcctaaaac gatttttcca ttgtctgaac tcatattttt tctttttaat
      541 tgtaacaaaa ataacaacgt ttcttaaatt atgttccctg tgcacaatta tttagcttct
      601 ctcatctttt ctgttgataa atagttggga aactgtcatt cgtcaacaat tgtaacaaag
      661 gtatttggta cctttcggct atatttccag gtaatattaa cttgaatgct atgtatggtg
      721 aattgaaaaa tcaattggtt aattctctca attcgcttag agagaatgga ttatataaag
      781 aagagcgaat catttcttct gaacaaggag ctgtgatcaa tgtagatggg gtgggcgagg
      841 tgctcaattt ttgttcgaat aattatcttg ggctggcttc aaaccgggag gtggtcgagg
      901 cggctaaaat ggcaatcgat cgatgggggt ttgggctttc atcggtgcgg tttatttgtg
      961 gtactcagca gattcataaa gaattagaac gtgcggtgtc tgatttctta ggaaccgaag
     1021 atactatttt gtattgtgca gctttcgatg ccaatggtgg tgttttcgaa cccttgtttg
     1081 atgaaaattg tgccataatt tccgacgagt tgaatcatgc ttcaataatt gacggggtac
     1141 gtttgtgtaa agccaagcga ttgagatata gacattccga tatggaaaat cttgaagaat
     1201 gtttgaagga ggcaaaagaa actacttaca agataattgt tactgatggt gttttctcca
     1261 tggatggaga tattgcgcgt cttgatataa tttgcagtct ggcagagcgc tatggggcat
     1321 tggttatggt cgacgatagt catgctacag ggtatattgg ccctacgggt aaaggaacag
     1381 ccgagctttt tggtttgaca ggcaagatag atatcattac ttcaacgttt ggcaaagcac
     1441 tgggtggtgg aaacgggggc ttcacctccg gacggcgcga aatcattgaa tacctgcgtc
     1501 aacgatcgag accttatctt ttctcgaaca cgttggctcc ggccacagtt ggagcaagcc
     1561 ttaaagtact cgaaatgctc gaaaactcta cttcactgtg cgatgctgta acacgaaatg
     1621 ccctctattt caggcaaaaa atagagcagg caggttttac tgtcaataaa ggtgagacgg
     1681 ctatagtccc tgtaatgatt ttcgatgccc cgatggctgt tcgttttgcc gatttgctgc
     1741 ttaatgaagg gatttatgtg attggatttg tatatcctgt tgtaccacaa ggaaaagcgc
     1801 gtatcagggt tcagttgtct gctgctcaca ctctaagcca gatagataag gctgttgatg
     1861 catttgtgaa aattggaaag cagttgcatg tgattaatta gcagttcttt atgatgttgg
     1921 ttggtggtta gtcctgattt tttgataaaa acatgtaaaa aagtataaca agtgtggttt
     1981 ttatacgttg taatccagat ttccaacgat tgtaaaaccc aaaaaatact acaatgggag
     2041 ccatagaacg aaagctgtac aaagtactgc gaaatgtagg agtgagaaaa aattacatat
     2101 ctaaagcaaa atctattgat gacctttttc ttgatgaatt cgaccgaaaa ttgctccgct
     2161 tttattttga aaatgagttt aaagtcaggc ttagcgaacg cgatgaattg aaactgacca
     2221 cactaaacga agtaaatggt tttttacggt gtactaaata attgaagttt tgtttttaca
     2281 aaataatagt ttgttcttcc ccggagaaaa gatttttttt tcggggaagt tttttgttct
     2341 ttttattgat ccctttttgt taacttgtaa tctgatttaa atggtttcga tatgaattac
     2401 actaaattat tccgaataaa tattgcactt gtgcttggag tgttatgtct tcaaattcat
     2461 gtatttgcac aaaatactga cgggcttata aatcagttga aggaatttcc tgaggtggtt
     2521 tctgttcaaa agattgaaaa taacccattc tttaaagaat cgtatgaaat atatatcgag
     2581 caatttctta accacaaaaa cccgtcggaa ggcaaattca ggcagcgagt tatattgtcc
     2641 gattacaata gatacagccc tgtggtgttt gttgccgaag gctatgatgc ggaatatgcc
     2701 atgaaacctt cctatatcaa tgaattgagc aaaattcttg aggctaatca acttgttgta
     2761 gagcatcgtt tctttggaaa atcggttccc gaaggcaaaa actggggtta tcttaccatg
     2821 caaagcgctt ccgatgattt gcaccgtatt cagcgtattt tcaaacgatt atacaataat
     2881 caaaataaat ggattgttac aggcattagc aaaggaggta caaatacttt ggcatacaag
     2941 gctttctatc ccgacgatgc tgatatttgg gtgccttatg tggggccggt taattttgcc
     3001 gtggaagatg gcaggcacga gaaattcctc aaagcagttg gttcgcaaac ttgtcgagaa
     3061 aagttactgc aatttcagcg ttcaattctt actcagcgag aagctatcca accccttttc
     3121 gacagcttgg taaaagcccg gaactattcg tttaggattc ctccggaaca agttctcgat
     3181 tattgtgtgc tggaatactc gtttgcattt tggcagtggg gtaccaattg tgccgctatc
     3241 cctgccgaca gtaccccgat acgcgttcag ctcaatcatc tggtagaagt atgttcgccc
     3301 gactattttt caaatgaggg gacagctccc atgcaatcgt tttttattca ggcagccaaa
     3361 gaaatgggct actatggcta cgatgtgaaa gcgcttaaac ctttactgaa aatcaaagat
     3421 gccgaagggt atttgtcaga gatatttatc cctgaaggac ttgcatttaa gtttgataag
     3481 aaaacctcca agtttatcga gaaaacagtc aatgccaacg gaaagcatgt tcttatgatt
     3541 tatggcgaaa cggacccatg gtcagcttcg ggcttcaatt tgaaaaaccc gaagagtgga
     3601 gccgtaaaaa tggtcatacc gaaagcttcg catagggtac gtatcgaaaa tatgccgcta
     3661 gccgaaaaag ctcaagtata tatgctactc gagtcttggt tgaacgaaga ttagcccctg
     3721 agtgcttcta tttcgctaat ctccattggt tttgtacggc ctagcaggcg gttcgaatcg
     3781 ggggttataa ggtaattctg ttcaattctg atgccaccaa aatcgaggta tttttcaact
     3841 tgagcatagt tgataaactg actaaacttt tgttcgcttt gccatagttt tattaactcg
     3901 ggaatgaaat aaatgcctgg ttcaatggtg agtacaaatc ccggctttaa ttctttggcc
     3961 agccttagcg agcgcaatcc gaattgagag cttttagttt ctccgttgta accaacgtta
     4021 atctcgccca aatcttccat gtcgtgcacg tcgaggccca tcatgtgccc taacccgtgg
     4081 ggaaaaaaca gggcatgggc tccttctgca atggcttctt cagtgttgcc ttttgtcaat
     4141 cccagcgatt tcagtccatt gaaaatgata cggcaggctt cgaaatgtac ttctttaaaa
     4201 gtaattcccg gtttcaggca tgaagcagaa ccgtagtggg catcgagcac aatttgataa
     4261 atttcgcgct gtttgtcgct gaattttttc gataccggaa aggtacttgt gaggtctccg
     4321 gcatagcacg atggtgttca gcaccggcat cgaggagaaa cagttgtcct tcttttagtt
     4381 ggttgccatg atagtggttg tggagtactg ctccgtttat cgaggctatt accggaaatg
     4441 aaatataacc ttgccgttcg aggggtattt gtgtgaccgc agcagcaatc tcggcttcga
     4501 acatcccggg gcgagccata ctgatggctt tttcgtgcat atcgacagtc cagtttaccg
     4561 ctttgtctat ttcagctatt tcaaattcat cttttatttc tcgttgactt ataacgactt
     4621 taacaaggtc ttccgacgat ttttggttaa actggtcggg cctgatatta agcagtcgta
     4681 ataattttat tttattctcg ggcctgaacg gggggagaaa atgaatgttt cggtcagaag
     4741 cttgtgcttc gcgtaaaatt gtatatagtt taacgatggg caacacagag ttaatcccga
     4801 ctttttggca ttcttcgctg agagtaggta cattgcccat ccaaataata tcgtcgatgg
     4861 taagctcatc gccaaaaaca atttcttcat cgttttcaac atctattatg gctgccaacg
     4921 atggccgatc gatgccaaaa taatataaaa atgaactgtg ttgacggaaa tgatatggat
     4981 tatcgggata attcatcgga ctttcgtcgt tgccaaaaaa aaggataaca ccttgtttta
     5041 tcttctcttt cagtattctg cgacgggtta tatacaattg cttatcgaac atgattcttt
     5101 agtttaactt tcaaaaataa tcgaaaaata ttcctcaacg gatgatccgt ttcaaattaa
     5161 ctaattttaa tactttcggt aaatgtagtg aagaattcaa tttactcata gctttgttta
     5221 ttgcttaaaa caaacaatct ttgta
//
LOCUS       DAOPUW010000041         5213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_15926_length_5213_cov_3.267352, whole genome shotgun sequence.
ACCESSION   DAOPUW010000041 DAOPUW010000000
VERSION     DAOPUW010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15926_length_5213_cov_3.267352"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>320
                     /locus_tag="PKJ43_01290"
     CDS             <1..>320
                     /locus_tag="PKJ43_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71220.1"
                     /translation="VLAGDYLKEASDSKINMVAVGLLYRYGYFKQTLNNKGEQIANYE
                     PEEFSKIPVQPVMNGEEWLSVSVTFPGRELRAKVWQVDVGSVKLYLLDADLQDNREED
                     RFVT"
     assembly_gap    321..330
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <331..2154
                     /gene="glgP"
                     /locus_tag="PKJ43_01295"
     CDS             <331..2154
                     /gene="glgP"
                     /locus_tag="PKJ43_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNZ71221.1"
                     /translation="DNENRLKQEILLGFGGIRLLRKLGFEADIYHCNEGHAAFIGLER
                     IHELITQNNLTFNEAKEVVRASTLFTTHTPVPAGHDAFHLDLLKSYGQGFPEKIQLSD
                     EEFLQLGRASVNEDHFNMSYLAANLSQNINGVSMLHGDVSRKLLKPLYPGYFPEELHI
                     GYVTNGVHYSTWAAKEWKELHKKYFGHNFPETQSDFKIWDKIQDVSDEEIFKLKQQLK
                     IKLMEYIKQRFADTWVQRNENPKLITEALSQFNPNALTVGFARRFATYKRAYLIFKNL
                     DRLNEIVNHPEHPVQFIFAGKAHPADKQGQDLIKYIVETSKRPEFIGKILFIQNYDMN
                     LAKMMLQGVDIWMNTPTRPLEASGTSGEKGVMNGTVHFSVLDGWWVEGYKPEAGWSLP
                     MEQSFDVPEFQDELDAETIYNIFEQEIIPAYYKRNKKGISETWISYVKNTISEVAPHF
                     TMRRQLNDYISKFYGPQADRYRMLIADDFKVARKISGWKGNVASKWDGIKLVEANVEH
                     GIAHTYKIGETHPAKITLDLNGLTSEDVGVEIVISKKHEERDVFVETQEFRIEHVDGN
                     YCTYCLDLTILTPGSYNYGLRVFPKNKLLPHRLDFQYLHWI"
     gene            2302..3246
                     /locus_tag="PKJ43_01300"
     CDS             2302..3246
                     /locus_tag="PKJ43_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HNZ71222.1"
                     /translation="MEPLQPNLKQHPVFFDMDGAIDDMIGLLTLMALDNCKLTGIALT
                     NANCFSECAVTQIQRIIGLFGQRDLQVAISNAEPVNQFPEKWRAKSRLLSKLALGPDD
                     ESQLGSISKMEAAQFTAEKILEQDENTTVVLTGPAANLINAIRLYPTVLHKIDRIVWM
                     AGAFLANGNVIAPDHDGSAEWNIFWDPASARDLLRTGVKIVLFPLDVCCQVSVDNYLL
                     YHLRENSHFRLSQLVCDMLEIAQNNHPEYCMWDVLPCLFVGYPDIVRLSNTSIDIEMR
                     GTSMGNMFKTSKGAPVHFASFVDDDKFYNCFIELMKKF"
     gene            complement(3364..3849)
                     /locus_tag="PKJ43_01305"
     CDS             complement(3364..3849)
                     /locus_tag="PKJ43_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ71223.1"
                     /translation="MQETNDSLIFVVEDNRVYNKLIVSYLKTNNFTNVMPFYSGEDAI
                     NNLYRNPDIIIQDFLLDGMNGIDVLKKAKEQNTNIEFIFLSGQDNIDVAINTMKYGAY
                     DYIVKDQMALKKLVNKINKIQSVNKLVKSNKRYRTGVVWFFIALAVIILIAILFIVLI
                     P"
     gene            4135..>5213
                     /locus_tag="PKJ43_01310"
     CDS             4135..>5213
                     /locus_tag="PKJ43_01310"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNZ71224.1"
                     /translation="MQTIIVILSGVSFSLEHRAYEQLKAFIDKISLHYSGYSNQRAIL
                     IEVERRIASYFTTNRLPDSDIINESLVQNAIDSLGNSVYSFENVTADQQPIYNSVTNT
                     SEPLSGSKLYRDTNSAIIGGVCSGLAQYLKTDKIIVRLLFVVFFIVSSGLVLLVYIIL
                     WIGMGKKSNILPPPASAGENNDLSGQRNFSQNLSGSASSENRYSQNVATEKPVKPSSF
                     NKVIGFLLFAFGILNLAALITGFVFLSEVVDKLPQVFHPLAQDGFFNHFFSPTSSSTM
                     VISALVVLGLPLLLLIYLGSRLMFNISTSGRPVVLSALAVWFIAIVVAISSVIGAVNV
                     FSTSATASETNSLAFASDTIYIKLAT"
BASE COUNT         1534 a          945 c         1047 g         1677 t
ORIGIN      
        1 cgttcttgcc ggcgactatt taaaagaagc tagtgactcg aaaattaaca tggtcgctgt
       61 tggtttgctt tatcggtatg ggtattttaa acagacactt aataacaaag gcgaacagat
      121 agcgaattac gaaccagaag aattctcaaa aatcccggtt cagccagtta tgaatggaga
      181 agaatggctc tctgtaagtg ttacgtttcc ggggagagaa ctccgtgcaa aagtatggca
      241 ggtagacgta ggcagtgtta agctttattt gctcgacgcc gaccttcaag ataaccgaga
      301 agaagaccgt tttgtaacac nnnnnnnnnn gataacgaga atcgacttaa gcaagaaatt
      361 ttgctcggtt ttggtggaat caggttactc cgcaaattag gtttcgaagc agatatctat
      421 cattgtaacg aagggcatgc cgcgtttatc ggacttgaaa ggattcatga attgataacc
      481 cagaacaacc ttacatttaa tgaggctaag gaagtggtta gggcatcaac actatttacc
      541 acacatactc cggttcctgc aggtcacgat gcttttcatt tggatttgtt gaaatcgtac
      601 gggcaaggtt ttcccgagaa gatccaactt tccgacgaag agttcttgca attgggaaga
      661 gcttcggtta atgaagatca tttcaatatg agttatcttg ccgctaactt atcccagaac
      721 attaacggag tgagtatgct acacggtgat gtaagccgta agttgttgaa accgctttat
      781 cctggctatt ttcccgaaga attgcatata ggctatgtta ctaatggagt gcattattct
      841 acctgggcag caaaagaatg gaaagaatta cacaaaaaat attttggcca taacttccct
      901 gaaacccaaa gcgatttcaa aatctgggac aaaattcaag acgtatccga cgaagaaatc
      961 ttcaagctaa agcaacaact gaagattaag ctgatggagt atatcaaaca acgttttgcc
     1021 gatacttggg ttcagcgaaa cgaaaatcca aagcttatta ccgaagcctt aagccagttt
     1081 aatcccaacg cgttaactgt gggttttgcc cgccgttttg ctacgtataa gcgagcttat
     1141 ttaatattta aaaacctcga tcgactgaac gaaattgtaa atcatcctga gcatccggtg
     1201 cagtttattt tcgcagggaa ggctcatccg gctgataaac agggacaaga tttaataaaa
     1261 tatattgttg aaacctcgaa gcgacctgaa ttcattggaa agatcctttt cattcagaat
     1321 tatgatatga atctggccaa aatgatgctt caaggggttg atatttggat gaatacacca
     1381 actcgtccgc tcgaagcttc ggggaccagt ggcgaaaaag gggtgatgaa cggtacagtt
     1441 cacttttcgg tactcgatgg ttggtgggtt gaaggctata aacccgaagc aggttggtct
     1501 ttgccaatgg aacagtcatt cgatgtgcct gaatttcagg atgaattaga tgctgaaacc
     1561 atttataaca tctttgaaca agaaattata cctgcatatt acaaaagaaa caaaaaagga
     1621 atttctgaaa catggatttc ttatgtgaaa aacacgattt ctgaagtagc tccgcacttt
     1681 actatgcgcc gacagttaaa cgattatata tcaaaattct atggaccgca agcagatcgt
     1741 tatcggatgt tgattgcaga tgactttaaa gtagcacgca aaatatcagg ttggaaaggc
     1801 aatgttgctt ctaaatggga tggaataaaa ttggttgaag caaacgttga acacggaatt
     1861 gcgcacactt ataaaattgg ggaaactcat ccagctaaaa tcacgctcga tttaaatggg
     1921 ttgacctctg aagatgtcgg tgttgagatt gtgatttcta agaagcatga agaacgagat
     1981 gtttttgtcg aaacacaaga gtttagaatt gaacatgtcg acggaaatta ttgtacatat
     2041 tgtctcgatc tcacgatact tactccagga agttataatt acgggctaag ggtttttcct
     2101 aaaaataagt tgctcccgca tcgactcgat tttcaatact tgcattggat ataaatatta
     2161 caaaaaaaac cgcggctagc ggtttttttt caactatttt ttatttcgaa gggcaaaaca
     2221 gttgtcaaaa ttcaacgttt tcaattttaa aattgtaatg atattatttt tacttttgta
     2281 cgttaacaaa tgaccaaatt gatggaacct cttcaaccta atttgaagca gcatcccgtt
     2341 ttttttgata tggacggtgc aattgacgac atgattgggc tattaaccct catggcttta
     2401 gacaattgta aactcactgg aatagcactt accaacgcta attgtttttc cgaatgtgcc
     2461 gtgacacaaa ttcagcggat aattggtctt tttggtcagc gtgatcttca ggtggccatc
     2521 agcaatgccg aaccggttaa tcagttcccc gaaaagtgga gggcaaaaag caggttattg
     2581 tcaaaattgg cattaggacc tgatgacgag tcccaattag gatcgatttc taagatggaa
     2641 gcagctcagt tcactgctga gaaaattctg gaacaagatg aaaatacaac agtagtactt
     2701 actgggccag cagcaaatct tattaatgct attcgtttgt atcctactgt tttacataaa
     2761 attgatcgga ttgtgtggat ggcaggggct tttttagcca acgggaatgt tattgctccc
     2821 gaccatgatg gaagtgcaga atggaatatt ttttgggatc cggcctcggc aagggacttg
     2881 cttcgcactg gtgtgaaaat tgttcttttc cctctcgatg tctgttgcca ggtttcggtg
     2941 gataattatt tattatatca ccttagagaa aacagccatt tcaggctttc acaattggtt
     3001 tgcgacatgc ttgagatagc tcagaataac catcccgaat attgcatgtg ggatgttctt
     3061 ccttgtcttt ttgtgggata tccagatatc gtgcgtttat caaatacttc gattgatatt
     3121 gaaatgaggg gaacttcgat gggaaatatg tttaaaacat caaaaggcgc tcccgttcat
     3181 tttgcctctt ttgtcgacga cgataaattt tataactgct ttattgagtt aatgaagaaa
     3241 ttttaaaaaa atgagattgg cgaaaccaat ctcattgtga aattttacaa acggtagggt
     3301 aaatataaaa ttactgaagg gatcttattc ttgaatcgta aaatatttgt tagtctgttt
     3361 acattatggg attaagacaa tgaataatat tgcaattaaa attataacgg ctaatgcaat
     3421 aaaaaaccat acaacgcctg ttcgatagcg tttgtttgat tttactaatt tattgaccga
     3481 ttgaatcttg ttgattttat tgaccagctt tttaagtgcc atttggtctt tcacaatgta
     3541 gtcatatgct ccatatttca tagtatttat tgccacatcg atattgtctt ggcccgaaag
     3601 gaatatgaac tctatattgg tgttttgttc cttagctttt ttaagtacat caatcccatt
     3661 cattccatca agcaaaaaat cttgaataat gatgtctgga tttcgataca aattatttat
     3721 tgcatcttca cccgaataaa aaggcattac attagtgaaa ttatttgttt ttaagtaact
     3781 cactataagc ttgttgtata ctctgttatc ttcaacaaca aaaattaatg aatcattggt
     3841 ttcctgcata ataaccagtt atgtttcaga tttgacgaat cccgattcct tctattcttc
     3901 gaaattacat taaaaaaatt aactgaccat ctaatttaca atttttattt cattcttttg
     3961 ttaaatcgcg gctttatttt ttaattccgg tgctcctata cgtgggttct gttctttcgt
     4021 ttacctgcta ttttatttca tttgccgata tatttattgc aaacaatcta gtttcgattg
     4081 gcgcttttgt tgcttagtct ttatctttaa tccaaaattg agaaatttat agtcatgcaa
     4141 acaattattg taatcctttc aggtgtttcg ttttcattag agcatagagc ctacgaacag
     4201 ttgaaagctt tcatcgacaa gatctcgctt cattactctg gttacagcaa ccagcgggca
     4261 atacttattg aggttgaaag gcgtattgcg tcatatttca ccacaaaccg tcttcccgat
     4321 agtgatataa tcaacgagag tttggttcaa aatgctatcg actcgctggg caactcggtg
     4381 tatagcttcg aaaacgtaac tgcagatcag cagccgattt acaattcggt aaccaatacc
     4441 tccgagcctt tatctggttc aaaattgtat cgcgatacca attctgccat tattggaggt
     4501 gtttgtagcg ggttggcgca atacctaaaa accgataaaa taattgttcg cttattgttt
     4561 gtcgtgtttt tcattgtttc atctggctta gtgcttttgg tatatataat tctttggatt
     4621 ggaatgggca aaaaaagcaa tattttgcct cctccggctt ctgcgggtga aaacaatgat
     4681 ttgtcggggc aacgtaattt ttcgcagaat ctttctggtt cagcaagctc tgagaatcga
     4741 tattcgcaaa atgttgcaac cgaaaagccg gttaaacctt ctagttttaa caaggttata
     4801 gggtttcttc tttttgcttt tgggatattg aacctggctg cattaatcac gggttttgtt
     4861 tttttatccg aagttgttga taaattacca caagtgtttc atcctctagc tcaagatggt
     4921 ttttttaacc actttttttc accaacatcg agttcaacaa tggttatttc agccttagtt
     4981 gtcctggggt tgccacttct gctattaatt tacttaggtt cccgattaat gtttaatata
     5041 tctacaagcg gccgcccggt tgttttatcg gcattggctg tttggttcat cgctattgtg
     5101 gttgccattt catcggtgat aggggcggtt aatgttttta gtacttcggc aacagcatcc
     5161 gaaacgaata gcctcgcttt tgcttccgat acaatatata ttaaacttgc aac
//
LOCUS       DAOPUW010000042         5153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_16199_length_5153_cov_2.873087, whole genome shotgun sequence.
ACCESSION   DAOPUW010000042 DAOPUW010000000
VERSION     DAOPUW010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16199_length_5153_cov_2.873087"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..618)
                     /locus_tag="PKJ43_01315"
     CDS             complement(73..618)
                     /locus_tag="PKJ43_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016779131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNZ71225.1"
                     /translation="MTEEIIIAGFGGQGVLSMGKILAYSGIMQDQEVSWFPSYGPEMR
                     GGTANVTVILSDERISSPVLNEYDTAIILNQQSLDKFESKIKAGGTLLFDPNGIIKPP
                     TRTDIKVYKIAATQIAAEQGKPRIFNMIVLGAFLQVKPIVKLENVQRGLEKSLPERHH
                     KTIPENMEALDLGKDNVIAVN"
     gene            complement(624..1439)
                     /locus_tag="PKJ43_01320"
     CDS             complement(624..1439)
                     /locus_tag="PKJ43_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNZ71226.1"
                     /translation="MADLQLQDIIKPENRVYGKSKVLTDKPMHYCYGCSHGVVNKLIA
                     EVIEEMGIQDKTVGVSPVGCAVFMYDYIDVDFMEAAHGKAPAVATGYKRTNPDKYVFT
                     YQGDGDLAAIGTGETFHAVNRGENIVMIFINNAIYGMTGGQMAPTTMLGQVTATTPYG
                     RDVDLNGYPLKITELLAQLPGAAYVTRQSVHTPANVRKCKKAIRKAFENSAKKIGTSF
                     VEVVSTCNSGWKIDPVASNKWMEENMFPFYPLGDLKDSERLDTEQYKVQKLKK"
     gene            complement(1442..2530)
                     /locus_tag="PKJ43_01325"
     CDS             complement(1442..2530)
                     /locus_tag="PKJ43_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNZ71227.1"
                     /translation="MGDLRLMKGNEVIAEAAIRCGCDGYFGYPITPQSEIMETLMARR
                     PWEDTGMVVLQAESEGASINMIYGGASSGKKVMTSSSSPGISLMSEGLSYLAGAELPC
                     LIVNVQRGGPGLGTIQPSQSDYFQATKGGGHGDFQLIVLAPASVQEMNDFVDLGFELA
                     FKYRNPAMILADGVIGQTMEKVELSDYKPRWTEQQIEEMHGSWAANGKKANRNQHIVT
                     SLELVSERQEKFNQKLQAKYRKIEEEEVRYEMIQCDDAEYIIVAFGLSARISQKSVDI
                     LRKKGIKVGLLRPITLFPFPTKIISELADRGVKGFLSVEMNAGQMVQDVRLAVNGKTK
                     VEYFGRMGGMITNPGEVADVVESKLIGK"
     gene            complement(2580..2804)
                     /locus_tag="PKJ43_01330"
     CDS             complement(2580..2804)
                     /locus_tag="PKJ43_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ71228.1"
                     /translation="MAKARGAVVVDNEKCKGCNLCVVACPYGVLNLNKEVNSKGYNYS
                     YMENPELCIGCTNCGMVCPDSCLTVYRVNS"
     gene            complement(2835..4085)
                     /locus_tag="PKJ43_01335"
     CDS             complement(2835..4085)
                     /locus_tag="PKJ43_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNZ71229.1"
                     /translation="MNRASIREVVQIVDMIEAETGEKFVRMEMGVPGLPAAKVGIDAE
                     IEALHRGVASLYPNINGIPELKTEAALFVKNFLDIDVKPEHCAPSTGSMQATYALFQV
                     SSCCDASKDTALFIDPGFPVQKQQFMMIGAKFESFDVYNFRGEKLREKLESYFSKGNI
                     HSVIYSNPNNPTWVCLTEDELKIIAELANKYDVIVMEDLAYFGMDFRKDISRPGVAPF
                     QSTVAKYTDNYVLFISASKIFSYAGQRIAVIVMSDKLFNRKYPALEKRFMMNTFGNTV
                     IYRALYALSSGVTHSVQFAMAAMLKAVNNGTFNFVEELKEYAEKAHIMKKLFTDNGFS
                     ILYDKDIDQDIADGFYFTLSYGTMKAGELLHELMCYGISAITLLSTGSKQEGLRACVA
                     PVKRSQFGDLEKRLKQFLADHPLN"
     gene            complement(4189..5034)
                     /locus_tag="PKJ43_01340"
     CDS             complement(4189..5034)
                     /locus_tag="PKJ43_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNZ71230.1"
                     /translation="MNIKKVAIQGIAGSFHEDAAQRYFGDEKIEIIECRTFRKVCQLV
                     DSDQADIAVMAIENAIAGSLLENYGLLRDYHLKITGEIFLHIQMNLLVLPGVKKEDIV
                     EIHSHPVAIKQCAEYIDATFGEISLDESFDTAGSAKNIAKNNLRNAACIANERSAEIY
                     GLEILDKGIETNKRNYTRFLILSKYYHHSDDANKASICFEVGHFYGSLAKVLNIFAEN
                     KINLTKIQSVPIIGKPDEYSFHVDIEWENSNNYEQAIHQVLKNVSSLTVLGEYVRGER
                     KIYDV"
BASE COUNT         1391 a         1193 c          977 g         1592 t
ORIGIN      
        1 caccaacaat ggtgccccta ttattttttc cggtaaaacc gggagaacaa aaccaatgcg
       61 aaattgctag tcttaattta ctgcaataac attatccttt cccaaatcga gagcttccat
      121 attttcagga atcgttttat gatgtctttc aggaagcgac ttttcaaggc cacgttgtac
      181 attttcgagt tttacaatag gtttcacctg caaaaatgca ccaagtacaa tcatattgaa
      241 tatgcgtggt ttcccttgct ctgcagcaat ctgagttgca gcaatcttgt acacttttat
      301 atcggtacgt gttggcggtt taataattcc gttcggatcg aacaaaagag ttcctcctgc
      361 ttttattttg ctttcgaact tatcgagcga ttgctgatta aggattatgg ctgtatcgta
      421 ttcgttcagc acgggcgaag atatccgttc gtcgctgaga ataactgtta cattggctgt
      481 acctccgcgc atttcaggcc cgtaggacgg aaaccatgat acttcctggt cttgcatgat
      541 cccagaataa gcaagaattt ttcccatcga cagaactccc tgtccgccaa aacctgcgat
      601 tataatttct tctgtcatcg taattacttt ttaagtttct ggactttata ctgctctgtg
      661 tcgagtcgtt cactatcttt caaatcaccc agcggataga atgggaacat gttctcttcc
      721 atccatttgt ttgaagccac cgggtcaatt ttccatcccg agttacatgt tgaaaccact
      781 tcaacgaacg aagtcccaat tttctttgct gaattttcga acgcttttct gatagctttt
      841 ttacatttcc gcacgttggc aggagtatga acactttgac gggtaacata cgctgctccg
      901 ggaagctgag ctagcaattc ggttattttg agtgggtagc cattcaaatc gacatcgcgg
      961 ccataaggag tagttgcagt aacctgtccc agcatggttg ttggggccat ttgaccgcct
     1021 gtcatgccgt aaatagcatt gttgatgaaa atcatcacaa tattttcacc ccggtttacg
     1081 gcatgaaaag tttctccggt gccaattgcc gcaaggtctc catcgccctg ataggtaaac
     1141 acatacttat cggggttggt acgtttatat cctgtagcaa cagcaggagc cttaccatgg
     1201 gctgcctcca taaaatcaac atcgatgtaa tcatacataa aaacagcaca tccaacaggc
     1261 gaaacaccaa ctgttttatc ctgaatgccc atttcctcaa tcacttcggc aataagcttg
     1321 ttaactaccc catgactgca accgtagcag tagtgcatcg gcttatcggt gagaactttt
     1381 gattttccat aaaccctgtt ttcgggttta atgatatctt gtaactgtaa atctgccata
     1441 gttatttccc gattaatttt gattcaacta catcggccac ttcgcccgga ttggtaatca
     1501 taccgcccat acggccaaag tattctacct ttgttttgcc attcactgcc aggcgtacat
     1561 cttgtaccat ctggcctgca ttcatctcta ccgaaaggaa acctttcact ccacgatcgg
     1621 cgagctcgct gataattttc gttggaaacg ggaaaagtgt aatcggacgt aaaagaccaa
     1681 cttttatgcc ctttttacgc aaaatatcaa ccgatttttg gcttattcgt gccgaaagac
     1741 cgaacgcaac gataatatac tcagcatcgt cgcattgaat catttcatag cgaacttctt
     1801 cttcctctat tttgcggtat tttgcctgca atttttggtt gaatttttcc tgccgttcag
     1861 aaaccaattc aagcgaagta acgatatgct gattgcgatt cgctttttta ccgttagccg
     1921 cccaacttcc atgcatttct tcaatttgct gttcagtcca gcgaggctta taatcactaa
     1981 gttccacttt ctccatggtt tgtccaataa ccccgtcggc aagaatcatt gccgggttac
     2041 ggtatttgaa ggcaagttcg aagcccaaat cgacaaaatc gttcatctcc tgaaccgaag
     2101 ctggcgccaa cacaataagt tgaaaatcac catgcccgcc ccctttggtt gcctgaaaat
     2161 aatcggattg cgatggctgg attgttccca acccagggcc tccacgctga acattaacaa
     2221 tgaggcaagg caactcagca cctgccagat aagataaccc ttcagacata aggcttatcc
     2281 cggggctcga cgatgaagtc attacttttt ttccggaaga agcacctcca tatatcatat
     2341 tgatagaagc gccttcactt tcggcctgta aaacaaccat gcctgtatct tcccagggac
     2401 ggcgagccat taaagtttcc attatttcgg attgaggggt aattggatat ccaaagtagc
     2461 catcgcatcc gcaacgtatt gcggcctcgg cgattacctc attccctttc attaaacgta
     2521 agtctcccat tgtatggatg attttaaagt ttctactaaa tttattattc ccgcgttctt
     2581 caggaattta ccctataaac agtcaaacaa ctatcgggac acaccattcc acagttggta
     2641 caaccaatac acaactccgg attctccata taagagtagt tatacccttt gctattcacc
     2701 tctttattaa ggtttaacac gccgtacgga caagcgacga cacaaaggtt acagcctttg
     2761 catttttcgt tgtcaactac tactgctcct cttgctttcg ccattgttct attttattta
     2821 ttatgtttat tctatcaatt taggggatga tcagccaaaa actgtttcaa ccgcttttca
     2881 aggtctccaa attgagaccg ttttactggg gccacacaag ccctcaatcc ttcttgcttg
     2941 cttccggtac tcaaaagtgt tattgcgctg atgccataac acataagctc gtgcaatagt
     3001 tcaccggctt tcatggtgcc atagctcagt gtgaagtaaa atccatcggc aatatcctga
     3061 tcaatatctt tatcgtataa aatactgaag ccgttatcag tgaaaagttt tttcatgata
     3121 tgggcttttt cagcatattc tttcagctcc tcaacaaaat taaaagttcc gttatttacc
     3181 gctttcaaca tggctgccat ggcaaattgt accgaatggg ttactcccga tgaaagtgca
     3241 tataaagccc tgtatataac cgtattccca aaagtgttca tcataaaacg cttttcaagt
     3301 gctggatact tacgattgaa aagcttatca gacataacga taacagcaat tcgctgccct
     3361 gcataggaga atattttcga tgccgagata aacaacacat aattgtcggt gtactttgcc
     3421 actgttgatt ggaaaggtgc cacgccagga cgcgaaatgt cttttctgaa atccatgcca
     3481 aaataggcaa ggtcttccat cacaataaca tcatatttat tggctaactc agcaataatt
     3541 ttcaattcat cttcggttag gcacacccag gttgggttgt tcgggtttga ataaattacc
     3601 gagtgaatgt ttccttttga aaaatacgat tcaagttttt cacgaagttt ttctccccgg
     3661 aaattataca catcgaacga ttcgaatttt gcaccaatca tcataaattg ttgcttctga
     3721 acaggaaatc ctggatcgat aaagagcgcg gtatcttttg acgcatcaca gcacgaagac
     3781 acttggaaca gcgcatatgt agcttgcatc gaacctgttg aaggcgcaca atgttcaggc
     3841 tttacgtcga tgtcaagaaa attcttcaca aataacgccg cttcggtttt gagttcagga
     3901 ataccattga tatttggata aagcgaagca acaccacggt gcaaggcctc aatctcggca
     3961 tcgattccta ccttggcggc tggcaacccg ggtacaccca tttccatacg aacaaatttt
     4021 tcgcccgttt cggcttcaat catatcaact atttgaacga cttcgcgaat cgaagctctg
     4081 ttcatatcgc tgatattcaa ggatgacaaa tgtttttcta cgatttccct cttgatggga
     4141 gttgattgta tcatgttctg ttaaactaaa ttagtggcaa tgcctttgtt atacatcgta
     4201 aattttccgt tcgccgcgaa catattctcc aagtacagtt aggctcgaaa cgtttttgag
     4261 cacctgatgg attgcctgtt cgtagttatt tgagttttcc cattcaatat caacatgaaa
     4321 cgagtactca tcgggttttc caataatggg aaccgattgt attttggtga ggtttatttt
     4381 attttcggca aatatattca atactttagc taaagagcca taaaagtgcc cgacctcgaa
     4441 acaaattgat gctttgttag catcgtcaga gtgatggtaa tattttgaaa ggatcaggaa
     4501 ccgggtataa tttcttttgt tggtttcgat tcctttgtca agaatctcga gtccataaat
     4561 ctcagccgag cgttcgttgg caatgcaagc ggcattcctg agattatttt ttgctatgtt
     4621 ttttgccgaa ccagcagtat cgaacgattc atcgagcgat atttcaccaa acgtagcatc
     4681 gatatattcg gcacattgtt taatagctac cggatgcgaa tgaatttcga caatatcttc
     4741 tttcttcacc ccaggcaaca ccaacaagtt catttgtata tgaaggaata tttcaccggt
     4801 gatctttagg tgataatcac gcagtaaacc gtaattttcg agtaagcttc cggcaatggc
     4861 gttttctatg gccatcactg caatatcggc ctggtcggag tcgacaagct ggcaaacttt
     4921 tctgaacgtg cggcattcta taatttctat tttttcatcc ccaaagtatc gctgggctgc
     4981 atcttcgtga aacgaccctg cgattccttg tatggctacc tttttaatat tcattgtgtt
     5041 tatcttttgt ttaaataata aaaaacccgg gtttagcccc gggttaagat aatatcactt
     5101 ggttatcact cctccgggaa caatttgcag caaatgccaa aaaatatgtc act
//
LOCUS       DAOPUW010000043         5109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_16383_length_5109_cov_3.078947, whole genome shotgun sequence.
ACCESSION   DAOPUW010000043 DAOPUW010000000
VERSION     DAOPUW010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16383_length_5109_cov_3.078947"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..560)
                     /gene="ribH"
                     /locus_tag="PKJ43_01345"
     CDS             complement(66..560)
                     /gene="ribH"
                     /locus_tag="PKJ43_01345"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNZ71231.1"
                     /translation="MATRDLSEYDISSVPQVEGMKVGIVVSEWNYHITSALAKGAVET
                     LLKHGLNDEDIIVRHVPGSFELTLGGQFFAEYTDVNAIILLGCVIQGETPHFDYVCQG
                     VTQGAVLLNTQYNLPVIFGVLTTSTEEQALDRAGGRLGNKGVEAAITAIKMYDLHIKM
                     MQDF"
     gene            complement(632..1312)
                     /locus_tag="PKJ43_01350"
     CDS             complement(632..1312)
                     /locus_tag="PKJ43_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ71232.1"
                     /translation="MVKDTHNKKAESFGEVEVALTKTEQFLEKHLNLVFYVIGGIIVV
                     VLGVLGINKYVISPKNVEAQEQMFAAQNFFSVDSFDVALNGDGVSLGFLDIIDDFGST
                     KSGKLAQYYAGISFLHLGDYNQAIKHLKKFKTDDLLLAPLAKAAIGDAYVELGNYDKA
                     ISAYNDALAVNNNDFTTPTIKLKLALVYEEKGNVEKALVILNEAKSEFPNNSEITAIE
                     KNIARLNQ"
     gene            1508..>2587
                     /locus_tag="PKJ43_01355"
     CDS             1508..>2587
                     /locus_tag="PKJ43_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNZ71233.1"
                     /translation="MYLKLLSILNYKNIEQSESQFSPKLNCFLGDNGAGKTNLLDAVY
                     YLSFCKSFFNAIDFQSIRHGADFFILQGKYSRNENDEIISCGYKAGQQKQFKRNQKIY
                     KRLSDHIGLFPLVMVSPSDSALIQGGSDERRRYIDSVISQYDPVYLDSLIRYNKALLQ
                     RNNLLKRFAETGKFDTETLSIYDEQLIIQGNIIFNKRIEFVNQLIPVFQDFYSTISGG
                     KEQVILEYVSTLHVDDFRTQLEESIDKDRITQFTNVGIHKDDLNLLLGAHPIKKIGSQ
                     GQNKTYLVALKLAQFEFLKQASGIKPILLLDDIFDKLDSNRVGKIIQLVAEDRFGQIF
                     ITDTDREHLQKLITDMNIDHAVFTIS"
     assembly_gap    2588..2597
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2598..2901
                     /locus_tag="PKJ43_01360"
     CDS             <2598..2901
                     /locus_tag="PKJ43_01360"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNZ71234.1"
                     /translation="NFKLMRRKETQRISDLIKEFTRNSHLDPKLQETRIIENWSNILG
                     PTIANSTRKIFISNKVLFVYIESPVIRHELFMMRSRLQEALNESVGENVIASIVFR"
     gene            2949..3731
                     /locus_tag="PKJ43_01365"
     CDS             2949..3731
                     /locus_tag="PKJ43_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71235.1"
                     /translation="MKSIIVLLLLIIVGLCAKGQKHSIPLNNYTWYTSNEILNSTDDL
                     NSFKSHLSAIDLSSFKPLGFDKVKLYTTIDGYDINNTKVFLEMQKTQSTIKADVNGKP
                     IENLRSDGNFTGEIENSESLDELLLCLTIELSEKNSVLDCISSIYLSTANQVSLGWAQ
                     PLADPFFEGYLLEVHIYNMLSKDIDGKLVARVFDQETLETVAENNNCAFSRAGSEAII
                     DVNFPEAKVNIADGSYLAEIMLLDKENNEAVVDKITIPIKSN"
     gene            3739..4917
                     /locus_tag="PKJ43_01370"
     CDS             3739..4917
                     /locus_tag="PKJ43_01370"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ71236.1"
                     /translation="MTNLRVLFATRRYIATAQLFLSLSLIFGTWVTYIPFISTKVGLS
                     KGDLGIVLLFGAIGSIFTLPFGKKLVSKIGEGKLALITTSLYAISMLGNFIADSFFLL
                     CVCLFVNGLSSALMQIASNSIVSTLEKKDNVSIMSSCHGFFSFGGLIAAGFGTMILIA
                     INNPLIHIILMVSIVLILQAVFASSYLPYKTESHVEKNETKHYKKPIKNVTLWGLASV
                     ALCVMLTEGAIADWSGLFLRDVVLVSPNIVGLGYAGFSIAMTLGRFVGDNLSSKLGAW
                     NVIVWGLALSIAGFILVLWANTFSTLSGFLLIGFGFSTIVPEVYRLSTNVKGVHPSSG
                     ISFMAGAGYFGFLTGPVLLGAIAENFGLKTSFLLLLALVTLGVIIATFIRRKKALTVK
                     "
BASE COUNT         1556 a         1004 c          945 g         1594 t
ORIGIN      
        1 aagcattttt cacatattgt ggcttattgc ctcaattttg gatgatttta tttgcaaact
       61 gttctttaaa aatcctgcat catttttata tgtaggtcat acatttttat ggctgttatc
      121 gccgcttcaa cccctttgtt tcccaaccga ccgccagccc tgtcaagagc ttgttcttcg
      181 gtactagtag taagaacacc aaaaataaca ggcaaattat attgagtgtt caaaagaaca
      241 gctccttgcg taaccccttg acagacataa tcgaaatgag gagtttctcc ctgaattaca
      301 cagcctaaca gaataatggc atttacatcg gtatattctg caaagaactg accaccaagg
      361 gtgagttcaa aacttccggg aacatgacga acaataatat cttcatcgtt cagtccgtgt
      421 ttcaacaagg tttccactgc acctttcgcc aaagcactgg taatatgata attccactct
      481 gaaacaacaa ttcccacttt cattccttca acttgaggaa ccgaactgat gtcgtattcg
      541 gataaatcgc gtgttgccat atttttttca ataataataa caaaaaacgg gcaaactttt
      601 ggttgcccgt tatatttttt tttgtaaaaa attactggtt aagcctggca atattttttt
      661 caattgcagt aatctcgctg ttattcggga attcgctttt tgcttcgttg agaataacaa
      721 gagctttttc aacattccct ttttcttcgt aaaccagcgc caacttcaat ttaatagttg
      781 gagtggtaaa atcgttatta tttacagcca acgcatcgtt gtatgctgaa atagctttat
      841 cataattgcc caattcaacg tatgcatcgc caatagctgc tttagctaaa ggggcaagca
      901 aaagatcgtc ggttttaaac tttttgaggt gtttgatagc ttgattatag tcgcctaaat
      961 gcaagaacga tataccagca tagtactgag ctaatttgcc cgatttggtt gagccaaagt
     1021 cgtcgataat atcgagaaaa cccagcgaaa cgccatctcc gttcaacgca acatcgaatg
     1081 aatcgacgct gaaaaaattc tgagcggcaa acatctgttc ctgtgcttcc acatttttag
     1141 gcgatatgac atatttgttt atgccaagca cacctaaaac aacgacaatt attccaccaa
     1201 taacatagaa tactagattc agatgttttt cgagaaactg ctcggttttg gtcaatgcta
     1261 cctctacttc accaaaactc tcggcttttt tgttatgagt atcttttacc attatgtaac
     1321 tgtttttcta aattgcccac aaaaatagtc ttttttgaaa aataccattc aaaatactta
     1381 acaattttcc tactcctgcg aaattcgctc tattcaccgt attttaagta atcgatattt
     1441 tatgattgaa gcgaaccttt cctttcgtgt ttagagtatt tttttcaatt ttgctaaaaa
     1501 agcaggcatg tacctaaagt tacttagcat tcttaactac aagaatatcg aacaatctga
     1561 aagtcagttc agcccaaagc tcaattgctt tcttggcgac aacggggcag gaaaaacaaa
     1621 cttgctcgat gccgtgtatt acttgtcgtt ctgcaaaagt ttttttaatg ccatcgattt
     1681 tcaaagcatc cggcatggtg ccgatttttt tatacttcaa ggcaaatact caagaaatga
     1741 aaatgatgaa ataatcagtt gtggatataa agccggtcag caaaaacagt ttaaacggaa
     1801 ccaaaaaata tacaaaagac ttagcgatca catcggattg ttcccattgg taatggtatc
     1861 tccatctgat tctgccctta ttcaaggagg aagcgacgaa cgccgcagat atatcgatag
     1921 tgttatctcc caatacgacc cggtttatct cgattcgctg atacgatata ataaagcact
     1981 gttgcaacgc aataatttac tgaagcgttt tgccgaaact ggcaaatttg atactgaaac
     2041 cctttctatt tatgatgaac aacttattat tcaaggaaat ataattttca acaagagaat
     2101 tgaatttgtc aatcagctta ttcctgtgtt tcaagacttt tattccacaa tatctggagg
     2161 aaaagaacaa gtaattcttg agtatgtgtc aacattacat gtcgatgatt ttcggacaca
     2221 acttgaagaa tcgattgata aagaccgaat tacacaattc acaaatgtag gcatccataa
     2281 agatgacctg aatttattgt taggtgcaca tccaataaaa aaaattggtt cgcaaggaca
     2341 aaacaaaacg taccttgttg cgcttaaact tgctcagttt gagtttttaa agcaggcttc
     2401 gggaataaaa cccattttac ttcttgatga catttttgat aaactcgact cgaacagggt
     2461 aggaaaaatt attcaattag ttgcagaaga tcgatttggt cagatattca ttacagatac
     2521 tgatcgagaa caccttcaaa aattaattac cgacatgaac atagaccatg cagtattcac
     2581 catttccnnn nnnnnnnaaa tttcaaactg atgcgacgca aagaaacaca acgaattagt
     2641 gatcttataa aagaatttac ccgaaattcg cacctcgacc caaaattgca ggaaacacgt
     2701 attatcgaga attggagcaa tatactggga ccaactattg caaattcgac acgaaaaata
     2761 ttcatctcaa acaaagttct ttttgtgtat attgaatctc cggtaatacg ccacgagctt
     2821 ttcatgatgc gaagccggct tcaggaagca ttgaacgaaa gcgtgggcga aaatgtaatc
     2881 gccagtatcg ttttcagata aaacatcaca aacaacacct taattagtag ttatctaaaa
     2941 agatatttat gaaaagcata atagttttac tgttgttaat cattgttggt ctttgcgcca
     3001 aaggacagaa acatagcatt cccctaaata attacacttg gtatacatcg aatgaaatat
     3061 taaatagtac ggatgatttg aacagcttca aaagccactt gtcagctatc gatttgtctt
     3121 catttaaacc tctcggtttt gataaggtaa agctctacac aacaatcgat gggtatgaca
     3181 taaacaatac aaaagtattt cttgagatgc aaaaaacgca gtcaacgatc aaggctgatg
     3241 tgaacggtaa acctattgaa aacctcagaa gcgacggaaa tttcactgga gagattgaaa
     3301 acagcgaaag cctcgatgaa cttttgttgt gcctgacaat tgaactgtcc gaaaagaatt
     3361 cagttcttga ctgtatttca agcatatatt tatctacagc gaaccaagtg agtctgggat
     3421 gggcccaacc cctcgccgac cctttcttcg aaggctattt actcgaagtt cacatttaca
     3481 atatgcttag caaagatatc gacggaaagc ttgtggcaag ggttttcgac caagagacat
     3541 tagaaactgt tgcagaaaat aataattgtg cattttccag agccggttct gaagcgataa
     3601 tcgatgtgaa ttttcccgaa gcaaaagtaa atattgccga tggtagctac ttggctgaaa
     3661 taatgcttct tgacaaagaa aacaacgagg cagttgtcga taaaattacg ataccaatca
     3721 agtccaatta acaagtaaat gacaaatctt agagttcttt ttgctacaag gagatacatt
     3781 gctacagccc agttgttcct ttctttaagc ctgattttcg gcacgtgggt tacctatata
     3841 cctttcatta gtacaaaagt tggcctgagc aaaggcgatt taggtattgt actgttgttt
     3901 ggagccatcg gctcaatttt taccctccct tttggcaaaa agttggtgtc gaaaattgga
     3961 gaaggaaaac ttgcacttat cacaacgtct ctttatgcta tttccatgtt gggcaacttt
     4021 atagctgact catttttcct tctttgtgtt tgtctgtttg tcaacggact ctcttctgct
     4081 cttatgcaaa ttgccagcaa ttccattgtc tcaactctgg aaaagaaaga taatgtttca
     4141 attatgtcat cgtgccatgg gttcttcagt tttggaggcc ttattgctgc cggctttggg
     4201 acaatgattt taattgccat aaataacccg ttgattcata taatattaat ggtatcgatt
     4261 gtgttaattc tgcaagcagt atttgcaagt agctaccttc cttataaaac cgaatcgcac
     4321 gtagaaaaaa atgaaaccaa acattataag aaacccatca aaaacgtaac gctatgggga
     4381 ttagcatcag ttgcactgtg tgttatgcta acggaaggtg ctattgccga ctggagtggc
     4441 ctctttctgc gtgatgtggt gttggtatct cccaacattg taggattggg gtatgccggc
     4501 ttttcaattg cgatgacatt ggggcgtttt gtcggcgata acctgagttc aaaattgggg
     4561 gcatggaatg ttattgtttg gggtcttgca ctgagcattg ccgggtttat tctagtacta
     4621 tgggccaata ctttttccac tttatccggc tttttattaa ttggctttgg cttttcaacc
     4681 attgttcccg aagtttaccg gctatcgacg aatgttaaag gtgttcaccc gtcgtcgggt
     4741 atttctttca tggctggtgc agggtacttt ggtttcctta ccggccccgt attgttaggg
     4801 gcaattgccg agaattttgg gttaaagact agcttcctgc ttttgctcgc cttggtgact
     4861 cttggggtaa ttattgcaac ttttatccga agaaaaaaag cactaacggt aaaataaaag
     4921 gacccgtttc ccggatcctt ttcattttca gaaatatgtg tgcttaaaat ttaaagctaa
     4981 agcctacgcc aaagagctct ttaaactgca cttttgcttt gttatctacc ggatcttttg
     5041 tatcgaagtc gtacagcatt tgagtactca actgggcact gatgtatttg ttgactttca
     5101 tattaattc
//
LOCUS       DAOPUW010000044         5073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_16546_length_5073_cov_3.365285, whole genome shotgun sequence.
ACCESSION   DAOPUW010000044 DAOPUW010000000
VERSION     DAOPUW010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16546_length_5073_cov_3.365285"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="PKJ43_01375"
     CDS             <1..870
                     /locus_tag="PKJ43_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71237.1"
                     /translation="LCFNVGQWGFGFFASSPLNFENFANGYLHFSLRTTSGELFRVTV
                     NGANSSKGEITFDGKNDPYGFIRDGKWHHIAVPMAVLTAQGLNLSACDNIFTMSGGTI
                     STLGIDNVYLSTSEIPLENPAICKTVSISVSPKSATGKTGVRKKFSATGKNQFDQLAD
                     VELTWECSGGTIDQDGYFTASDAGEYTVTARMDTLSDSATINILSSVSAQVFENEITV
                     KHLPLTNKIEINGLEPNCRISVFNLLGEELYNSQAGPGFYEIDLEKVAVNSVCIINIR
                     KGEKSKTVKISAH"
     gene            complement(928..1839)
                     /locus_tag="PKJ43_01380"
     CDS             complement(928..1839)
                     /locus_tag="PKJ43_01380"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNZ71238.1"
                     /translation="MNIWEELKSTFKHGSVLTRLIYVNLAVFLAVRILHVFLFLFKVD
                     FDFLQWLALPSDISQLASRPWTLVTYMFLHFDFLHILMNLLWLYWFGKIFLMYFDEQK
                     LFGTYVAGGITGGVLYVLAFNFFPAFAEIAHQTILLGASASIIAIVVAAAVYVPNLSV
                     NLLLISSIFGPIKIVWIALFSLLIYFLSITGSNAGGNIAHLGGALWGFIYMSQLKHGR
                     DFMKGFNRFLFSFGSWFQSRKTMRVSYRRKSVQRMSDREYNLKKKTEKENINTILDKI
                     SKTGYDSLSKHEKEILFKMGSNKGRPN"
     gene            complement(1897..2733)
                     /locus_tag="PKJ43_01385"
     CDS             complement(1897..2733)
                     /locus_tag="PKJ43_01385"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNZ71239.1"
                     /translation="MASSFRSSGTQIPPVTKNLIIINAIMLFATYLLEPKGLSLTRYL
                     GLHYPQSEYFHFYQFITHMFMHGGITHLFFNMFALWMFGRVLESVWGPKRFLIFYFVT
                     GLGAAALHTFVNYLEISPIAHAVSAFSNTPSADLFSSFVNKNQSAIKEMGFNLPGIID
                     FATAWLDNPTSTSYEGESVAFMQQFLSMKMNIPTVGASGAVYGVLLGFGMLFPNTQLM
                     LLFPPIPIKAKWMVIAYGALELVLGLSMTGSNIAHFAHLGGMIFGFFMIKYWNKTTNT
                     FY"
     gene            complement(2839..4695)
                     /gene="mutL"
                     /locus_tag="PKJ43_01390"
     CDS             complement(2839..4695)
                     /gene="mutL"
                     /locus_tag="PKJ43_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010423251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNZ71240.1"
                     /translation="MSDIIHLLPDSVANQIAAGEVIQRPASVVKELVENAIDAGATKI
                     TVQAKDSGRTFIQVTDNGKGMSPTDARMAFERHATSKIREANDLFAIRTMGFRGEALA
                     SIASVADVELKTRRHDDEVGTFIHISGSELISQEVISCSEGTTFMVKNLFFNVPARRK
                     FLKSNNTELRHIISELQRVALATPEVGFSLYHNGTVVFELPAGNFRKRIVSLFGNSMN
                     QSLVPVQTETSIARIFGFIGQPKFARRTFGEQYFFVNGRYMKHPFFHKAVIQGYEKIL
                     PPDLIPSYFICFEVDPGSIDINIHPTKTEIKFESESAVWQMLHAAVREALGKFNVGPS
                     IDFDQEGNPGIPISLKSTDGLVFPSIQYNPNYNPFDSEPASVSYRKSGFSNSDSNLEN
                     WKDLYPSPMSDATFDVKPDDEVTQQQFFADTKDSSLAFDDSKKFIQLKNRYILTPVKS
                     GLMVIDQRRAHQRILYEQFLSIMAHHEGVSQQLLFPQTIELNSSDAAILRSMFDDLNT
                     IGFDIREFGPDTFILNGVPGVLGDIDAKGIIETFLEEFKSSVFDIKEKARERLACSLA
                     KASAIEYGYQLSKEAVSCLIDSLFACQSPNYSPTGKPVVDIIPFDEFEKRMK"
     gene            complement(4707..4991)
                     /locus_tag="PKJ43_01395"
     CDS             complement(4707..4991)
                     /locus_tag="PKJ43_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71241.1"
                     /translation="MGNFFKLPKPRQFKVEPRYWDPEKEKREARERRIRSDLGLLDES
                     GAYRPYISREDFRKGFSNGRWMDKKQRQRSNNRLLFIILLLAALAFFMLR"
BASE COUNT         1670 a         1098 c         1000 g         1305 t
ORIGIN      
        1 ttatgcttca atgtcgggca gtggggcttc ggtttttttg cttcttcccc attgaatttt
       61 gaaaattttg ccaacggtta tcttcatttc tcgttgagaa caacatctgg cgaacttttc
      121 agggttactg taaacggagc aaattcctct aagggcgaaa taacattcga tgggaaaaat
      181 gacccctatg gcttcattcg cgacggaaag tggcatcata tagcggtccc catggccgtt
      241 ttgacagctc aagggctgaa cctttcggcc tgcgacaata tttttaccat gtcaggcgga
      301 actatttcaa ccttaggcat cgacaatgtt tatctgtcga ctagtgaaat accgctagaa
      361 aaccctgcaa tttgcaaaac cgtatcgatt agcgtatcac caaaaagtgc aacagggaaa
      421 acgggcgtta gaaagaaatt ttcagcaaca ggcaaaaatc aattcgatca attggccgat
      481 gttgagctca catgggaatg cagcggggga accatcgatc aggatggata ctttacagcc
      541 tcggatgccg gagaatacac cgttactgcc cgcatggaca cgctcagcga cagtgccact
      601 atcaatatcc tatcgtcagt ttcggcacaa gtttttgaga atgaaattac agtcaaacac
      661 ctgccattga caaataaaat tgaaataaac ggactggaac cgaattgtag gattagtgtc
      721 ttcaatcttc tgggagaaga actttacaat agtcaagcgg gcccgggttt ctatgaaata
      781 gaccttgaaa aagttgcagt aaattcagtg tgcataataa acatcaggaa aggagaaaaa
      841 tctaaaacgg taaaaatttc agcacactaa cgacaaacac atttagaatt aagttggaga
      901 tgttttccct tgctaaggat actgctatca attagggcgg cctttgttac tgcccatttt
      961 gaaaagaatt tccttttcat gtttcgacaa gctatcgtaa ccggttttgc tgattttatc
     1021 gagaatagta ttaatgtttt ctttttcagt ctttttcttg agattgtatt cccggtcaga
     1081 catgcgctga accgatttac gccgataact tacgcgcatt gtctttctcg actgaaacca
     1141 ggaaccaaag gaaaaaagga agcggttaaa acctttcata aaatcacgcc catgcttcag
     1201 ctgagacatg taaatgaaac cccagagtgc accgcccaaa tgagcaatat tccctccggc
     1261 attagagcct gttatgctca gaaaatatat cagaagcgaa aacaaagcaa tccagacaat
     1321 cttgattgga ccaaatattg aactgataag taataaatta acagaaagat tagggacata
     1381 tactgctgcc gccaccacta tggcaataat agatgcagaa gctcccagca aaatcgtttg
     1441 atgggcaatt tcggcaaaag cgggaaagaa attaaatgca agaacataca aaacgccacc
     1501 agtaataccc cctgcgacat aagtaccaaa gagtttttgc tcatcgaaat acataaggaa
     1561 aatttttcca aaccaataaa gccagagcaa attcataaga atatgcaaaa aatcgaaatg
     1621 gagaaacatg taggtgacta atgtccacgg acgagaagct aactgcgaga tgtctgatgg
     1681 tagtgccagc cactgcagaa aatcgaaatc aaccttgaaa agaaataaaa aaacatgaag
     1741 aatgcgcact gccaaaaaaa cagcaaggtt tacatatatt aagcgtgtaa gtaccgagcc
     1801 atgcttaaaa gtgcttttca attcttccca aatattcata ctcaacaaat tattaatctg
     1861 tttcttacca caaaaatctt attcaaaaaa aattgattaa taaaaagtat tggtcgtttt
     1921 gttccaatat tttatcatga aaaacccgaa aatcattcca ccaaggtgag caaaatgggc
     1981 aatattacta ccagtcatcg agagcccaag caccaattcc aatgcaccgt aagcaataac
     2041 catccacttt gctttgatag gaattggagg gaaaaggagc attaactgag tattcgggaa
     2101 aagcatacca aaaccgagca aaacaccata tacagccccc gaagccccaa cagtaggaat
     2161 attcattttc atggaaagaa attgctgcat aaacgcaaca gattctcctt cgtaagaagt
     2221 tgatgtcgga ttatcaagcc atgctgtagc aaagtcgata atacccggta agttgaatcc
     2281 catctccttt attgctgatt gatttttgtt aacaaatgac gaaaacaagt cggctgaagg
     2341 agtatttgaa aaagcggaaa ccgcatgagc tattggacta atttcaagat aattaacaaa
     2401 agtatgcaat gcagcagcac ccaagcctgt aacaaaataa aaaatcagga aacgctttgg
     2461 cccccaaaca ctttccagca cgcgtccaaa catccacaag gcaaacatat tgaagaacag
     2521 gtgggtaatt cccccatgca taaacatgtg agtaataaat tgatagaaat gaaaatactc
     2581 agactgagga taatgcaacc ccagataccg tgtaagacta agaccctttg gctcaagaag
     2641 atatgtcgca aacaacataa tcgcattgat aatgataagg ttcttggtaa ccggaggtat
     2701 ttgtgtacca gatgaccgaa atgaagatgc catatgttaa ttatttaatc atttttctac
     2761 tagtaattat ctgtttaaca attatcgagc cattaagttt gacacaaaaa tagaatgata
     2821 atactgccaa catgtcaatt acttcattct tttttcaaac tcatcgaacg gaataatatc
     2881 aaccacaggc tttcccgttg gtgaatagtt tggtgactga caagcaaaca acgagtcaat
     2941 caagcaactt acggcttctt tcgaaagctg atagccatat tctatagccg aagccttggc
     3001 caacgagcaa gcgagccgct cacgggcttt ttcttttata tcgaatacac tagatttaaa
     3061 ctcttcgaga aacgtttcaa ttatgccttt tgcatcaatg tcgcctaaaa caccgggaac
     3121 cccattgagg ataaacgtat cgggaccaaa ctcgcgaata tcgaaaccga tagtattcag
     3181 gtcatcgaac atagaacgta atattgcagc atctgaacta ttaagctcaa tagtttgagg
     3241 aaagagaagt tgctgactaa ccccctcatg atgagccatt atagaaagaa actgctcata
     3301 caaaatccgt tgatgcgccc ttcgctggtc aatgaccatc aaccccgatt tcacaggagt
     3361 aagtatatat ctgtttttca actgaataaa cttcttagaa tcatcaaatg ccagcgaaga
     3421 atctttcgta tcggcaaaaa actgttgctg agtaacttcg tcatcgggtt ttacatcgaa
     3481 cgttgcatca gacattgggg aaggatataa atctttccag ttttccaaat tactgtcaga
     3541 attcgaaaat cctgacttac gataactcac tgaagcaggt tctgaatcga acggattata
     3601 attcggatta tattgaatgg agggaaagac aagcccatca gtcgatttta gggatatggg
     3661 tattcccggg ttcccttcct gatcgaaatc gatgcttggc cctacattga attttcctaa
     3721 agcttcgcgc acagccgcat gaagcatctg ccaaacagca ctttccgact caaatttaat
     3781 ttcggttttg gtcggatgaa tattgatatc gatacttccc ggatccacct cgaaacaaat
     3841 aaaatacgaa ggaatcagat caggaggcag gattttttcg taaccctgaa tcactgcttt
     3901 atggaagaac gggtgtttca tatatcgccc attcacaaag aaatattgct caccaaacgt
     3961 tcgccgggca aactttggtt ggccaataaa tccaaaaata cgggctattg aggtttcggt
     4021 ctgaacaggc acaaggctct ggttcatcga gttaccgaac aaactaacaa tccgtttgcg
     4081 gaagttccca gcaggcaatt caaaaacaac tgttccatta tggtatagcg aaaacccaac
     4141 ttcaggggtc gcaagagcca ctcgttgcaa ttcagaaatg atatgccgca attcggtatt
     4201 gttcgacttc agaaacttac gccgcgccgg aacattgaaa aacaggtttt taaccatgaa
     4261 ggttgtaccc tcggaacaag aaataacctc ttgcgaaata agttcagaac cagaaatatg
     4321 gatgaaagtt cccacctcgt cgtcgtgcct gcgggttttc agttcaacat cggcaaccga
     4381 ggcaatcgaa gccaacgctt cgccccgaaa ccccatagta cgaatggcaa acaaatcgtt
     4441 agcctccctg atttttgaag tggcatgcct ttcgaaagcc atacgggcat cggttggcga
     4501 cattcccttg ccgttatcgg ttacttgaat gaaggtccgg ccagaatctt tagcctgcac
     4561 cgtaattttt gtagccccgg catcgattgc attttcaaca agttctttca ccaccgaggc
     4621 agggcgttga ataacctcac cggcagcaat ctggttggca acagaatcgg gcaaaagatg
     4681 tataatatcg gacacagcag aaaagtttaa cgcaacataa agaaggctaa agcagccaac
     4741 aaaagaataa taaagagcaa ccggttattc gaacgttggc gctgcttttt atccatccac
     4801 cggccgtttg aaaaaccttt tcgaaaatct tcgcggctga tatacgggcg atatgctccc
     4861 gattcatcca gaaggcctaa atcggaacgg attcggcgct cacgagcctc acgtttttcc
     4921 ttttcaggat cccagtaacg cggctcaacc ttgaactgac gtggctttgg cagcttaaaa
     4981 aaatttccca taaattctct tttgttatca tacaaagata aggctttttt catggttcag
     5041 aaacgactta aaagaagcca tattcaaaaa gaa
//
LOCUS       DAOPUW010000045         5057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_16622_length_5057_cov_3.432427, whole genome shotgun sequence.
ACCESSION   DAOPUW010000045 DAOPUW010000000
VERSION     DAOPUW010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16622_length_5057_cov_3.432427"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PKJ43_01400"
     CDS             complement(<1..204)
                     /locus_tag="PKJ43_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71242.1"
                     /translation="MKKIFLLLICFNLLLCGCTSFYQLVNISPSSKLKEISFANEAPN
                     SLYEFYYSDTLANDYLAEIRNLYN"
     gene            complement(533..2284)
                     /locus_tag="PKJ43_01405"
     CDS             complement(533..2284)
                     /locus_tag="PKJ43_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HNZ71243.1"
                     /translation="MNTRNAMILLVLFSFASMSGFSQNKKQKTKQPTNVPTFTSAVYT
                     GSDQVYIDNPLKEGEFYNPILQGCYPDPSITKKGEDYYLVCSTFAMFPGVPIFHSKDL
                     VNWTQIGHVLDRVSQLEVHDCGISAGVYAPAIKYNPNNDTFYMITTEFSGGFGNIVVK
                     TKDPMKGWSDPYKLQFNGIDPSLFFDDNGKAYVVHNDAPDQGKELYEGHRVIKIWEYD
                     VEKDQVIAGTDKIIVDGGVDLSKKPIWIEAPHIYKRNNKYYLMCAEGGTGDWHSEVIF
                     ISDNVKGPYVPAPSNPILTQRHFPKDRANKVDWAGHADLVEGPDGKFYGVFLAIRPNE
                     KDQVNIGRETFILPVDWSGTFPVFENGMVPMEPKLKTPKGVQYKGGQDGFFPNGNFTI
                     TENFTSEKLDYRWIGLRGPREAFITLTGKGLKINPFPTTVKEVKPTSTLFHRQQHSNF
                     SFTATMEYTPLSEKDLAGITCLQSESFNYVFGVTKKADEYYLVLEKNQRKGRFGRAIE
                     SEQVGTVKIDVKAPITLQVKAKGAEYEFSYSTNGTDFINVGGTVPGDILSTNVAGGFT
                     GSLIGLYATSANDIVIK"
     gene            complement(2339..4801)
                     /locus_tag="PKJ43_01410"
     CDS             complement(2339..4801)
                     /locus_tag="PKJ43_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HNZ71244.1"
                     /translation="MRKVKLMLVLLGVFCFAACQSSEPQGGKLKMWYDKPAKVWNEAL
                     PVGNGRLGAMIYGDPATEKVQLNEETFWSGGPSRNDNPKALEALPKIRELIFSGKFKE
                     AEAMVNENMVTQLHGSKYQVVGDLILNFPGHENFTNYYRELDLERAIFKTTYQVDGVT
                     YSREVFASQPDQVLVVKLSASKKKKLNFSAHLQGPLQKENRTLDDKTLEMTGLSSTHE
                     GVEGQVKFCTRAKILANGGSVSADSATINVKDASEVTILISMATNFVDYKTLTANEVE
                     KCSNYLAKAEKVPFNKMLDRHIAAYQSYFKRSSFDLGTSPAAQQPTDVRIKNFSKTYD
                     PELVSMYYQFGRYLLISSSQPGGQPANLQGIWNESTNPAWDSKYTININAEMNYWPAE
                     KCNMSELHEPLIQMVRELSETGRQTARDMYACDGWVAHHNTDIWRISGVVDGAFWGMW
                     PMGSAWLSQHLWEKYLYNGDLKYLESVYPIMKSACEFYQDFLIEEPTHKWLVVSPAIS
                     PENAPAGRTSICAGATMDNQILFDLFSKTIKTASLLNKDAELMGEFKKILDRLPPMQI
                     GQHGQLQEWIEDLDNPKDEHRHVSHLYGLYPSNQITPYATPELFDAARTTLKHRGDVS
                     TGWSMGWKVNFWARLLDGNHAMKLIKDQLTLVDPVRPGRNGGTYPNFFDAHPPFQIDG
                     NFGCTAGIAEMLMQSHDGSIHLLPALPDEWKTGEITGLRAPGGFEVSFSWSDGKVQRV
                     EIKSNLGGNCRIRVPNALLTAKGAVLDVAQAANPNPFYEVSGVKKPIVSEAAKLNALQ
                     LSETQLYDLPTEAGKVYSLKIK"
     gene            complement(4852..>5057)
                     /locus_tag="PKJ43_01415"
     CDS             complement(4852..>5057)
                     /locus_tag="PKJ43_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5110 domain-containing protein"
                     /protein_id="HNZ71245.1"
                     /translation="NYEKGQFSTIQFTWDDAKKTLTVADRSGSFPGMLKKRIFNVVLV
                     KAGENLQRKSVDYQGEKTKVSFL"
BASE COUNT         1346 a         1171 c         1030 g         1510 t
ORIGIN      
        1 attatacaaa ttcctaatct cagcaaggta gtcgttcgcc aaggtgtcgg aataataaaa
       61 ctcataaagc gaattaggcg cttcattggc aaacgaaatc tctttcaatt ttgacgacgg
      121 gctaatattt accagctggt aaaatgaagt acagccacat aataaaaggt taaaacaaat
      181 aagaagcaaa aagatttttt tcataggtat actattaaaa tttaacaatc acttatttat
      241 acacaaaagg cgcagctatt tacacgctgc atgaaaaaca caatcaccca aaagttactg
      301 ccctactcaa tactaaaata aattggatgg tacttttcac gttaagccct aatgtattaa
      361 aaggtgcaac acacgcttaa tgcaaccctg cccaatggaa aaacctcacc cttggtgctt
      421 taattgatgc taaagtaata attctttaga taaatcatcc tggttaatta aatacaaaag
      481 cagtcccgag ttcgagactg cttttaacca caaaaaaaat tagtctgttg atctatttaa
      541 ttactatatc gttggcagag gtagcatata atcctatcaa actaccggta aaaccacctg
      601 ccacattggt tgaaaggata tcgccgggaa cggttccgcc tacatttatg aaatcggttc
      661 cattagtgga atagctaaac tcatattcag cgccttttgc tttcacttgc agcgtgattg
      721 gagcttttac gtctattttt acagtaccca cttgttcgct ttcaatagcg cgtccaaacc
      781 ggcctttccg ctggttcttc tcgagtacca aataatattc gtcggctttt tttgtaacgc
      841 cgaacacata attgaaactt tcgctttgca aacaggttat gccagccaaa tctttttcac
      901 taagcggcgt atattccatg gttgccgtaa aagagaaatt gctgtgctgc tggcgatgaa
      961 acaatgttga tgttggtttc acttctttaa ctgttgttgg gaaaggatta attttcaacc
     1021 ccttaccagt aagagtaatg aatgcttcac gcggtccacg caagccaatc cagcggtaat
     1081 ccaatttttc ggaagtgaag ttttcggtaa ttgtgaaatt tccgttaggg aaaaatccat
     1141 cttgtccgcc tttatattga acccctttgg gtgttttcaa tttgggctcc ataggtacca
     1201 ttccgttttc gaaaacgggg aaagtaccgc tccagtcaac cggtaaaata aatgtttcac
     1261 gaccaatatt tacctgatct ttttcattcg ggcggatggc caaaaataca ccatagaatt
     1321 tgccgtcggg accttcaacc aagtcagcat gaccggccca gtctacttta tttgccctgt
     1381 cctttggaaa atggcgctgc gttaaaattg ggttgctggg tgcaggaaca taaggtcctt
     1441 tgacattgtc gcttatgaaa ataacttcac tgtgccaatc gccagttccc ccttcggcac
     1501 acattaaata atacttgttg ttacgcttat aaatgtgcgg agcttcaatc cagatgggtt
     1561 ttttcgaaag gtcaactccc ccatcaacaa taattttatc ggttccggca attacctggt
     1621 ctttttccac atcgtattcc caaattttta ttacccggtg accttcataa agttctttcc
     1681 cctggtcggg agcatcgttg tgtaccacat aagccttgcc attgtcgtcg aaaaacaacg
     1741 aagggtcgat tccgttgaat tgcagtttgt acggatcgct ccagcctttc atcggatctt
     1801 tggtctttac tacaatgtta ccaaatccac ccgagaactc cgtggtaatc atatagaacg
     1861 tgtcgttgtt cggattgtat ttgatggctg gtgcatacac tccggcacta atgccacaat
     1921 catgaacttc caattgagaa acccgatcga gcacatgccc aatttgtgtc cagttcacca
     1981 aatcttttga gtggaaaatt ggtacccccg gaaacatggc aaaagttgaa catacgagat
     2041 aataatcttc acccttcttt gttatcgaag ggtcgggata acacccctgt aaaatgggat
     2101 tataaaactc gccttccttc aacgggttgt cgatatacac ctggtcactg cctgtgtaga
     2161 ctgcactggt gaatgttggc acattagtcg gctgtttggt tttctgcttt ttgttttggc
     2221 taaaaccaga catactcgca aatgaaaaca ggactaacaa aatcattgcg tttcttgtgt
     2281 tcattgaaaa acttgttaaa tctttcttca tcatcaatct atattctttt gttatcaatt
     2341 attttatttt taatgaataa actttaccgg cttcggttgg taaatcataa agttgtgttt
     2401 cggacaattg aagggcgttc aattttgctg cttcggaaac aataggcttt ttaacccccg
     2461 aaacttcgta gaacgggttt ggattggcag cttgtgcaac atccaacact gcccctttgg
     2521 cggttagcag cgcgttaggt accctgatgc gacaatttcc gcccaaattc gacttaattt
     2581 caacacgctg aactttgcca tcactccacg aaaaacttac ttcgaaacca cccggagcac
     2641 gcaagcccgt gatttcccct gtcttccatt cgtcgggcaa agcgggtaac aagtgaatgg
     2701 aaccgtcgtg gctttgcatc agcatttcgg caataccggc tgtgcagcca aaattcccgt
     2761 cgatttggaa aggggggtgc gcatcgaaaa agttgggata tgtgccgcca ttacgtcccg
     2821 gacgaacggg atctaccagg gtcagctggt ctttgatcag cttcatagcg tggtttccgt
     2881 cgagcaggcg tgcccaaaaa ttcactttcc agcccataga ccacccggta gatacatctc
     2941 ccctgtgttt caacgtagtg cgggccgcat cgaacaactc gggcgtggca tacggcgtaa
     3001 tttggttgct tggataaagt ccatacaaat gagacacatg gcggtgttcg tctttcggat
     3061 tgtccaaatc ttcaatccat tcttgcaact ggccatgttg cccaatttgc atcggcggca
     3121 aacggtcgag tatttttttg aattcgccca tcagttctgc atctttattc agcaagctgg
     3181 ctgttttgat ggttttcgag aacaagtcaa ataaaatttg attgtccatg gttgctcctg
     3241 cacaaatgga agtcctgcct gcaggcgcat tttcgggcga aattgccggg cttaccacca
     3301 accatttgtg tgtaggctct tcaatcagga aatcctgata aaactcgcaa gccgatttca
     3361 taatgggata aaccgattcc aggtatttta aatcgccatt atacaaatat ttttcccata
     3421 aatgttgaga aagccaggcg ctacccatag gccacatgcc ccaaaaagca ccgtctacaa
     3481 caccactgat acgccaaatg tcggtattgt gatgagccac ccagccatcg caagcgtaca
     3541 tgtcgcgcgc tgtttgccgg cctgtttctg acaattccct caccatttgg ataagcggtt
     3601 cgtgcagctc agacatattg catttttcag caggccaata attcatctct gcattgatat
     3661 tgatggtgta tttgctgtcc catgcaggat tggtactttc gttccaaatc ccttgcaggt
     3721 tggctggttg tccgccgggt tgcgagcttg aaatgagcaa ataacgtcca aactggtaat
     3781 acatcgaaac caactcggga tcgtaggttt tcgaaaagtt cttgatgcgg acgtcggtgg
     3841 gttgctgtgc ggctggcgac gttcccaaat cgaagctaga acgcttaaaa tatgattgat
     3901 aagctgcaat gtgccgatcg agcattttat tgaacggaac tttttcggcc ttggctaaat
     3961 agttcgagca tttttcaact tcattggcag ttaatgtctt ataatcgaca aagttggtcg
     4021 ccatcgaaat gagaatggtt acctccgaag catcttttac gttgattgtt gcagaatcgg
     4081 cagaaacaga acccccgttg gccaaaattt tagcccgggt acagaactta acttgtccct
     4141 caacaccttc gtgcgtactg gaaagccctg tcatttcgag ggttttatcg tccaatgtgc
     4201 ggttttcctt ttgaagcgga ccttgtaagt gtgccgagaa attgagtttc ttctttttgc
     4261 tggccgaaag tttcaccaca agtacctggt ctggctgtga cgcaaacact tcgcgggagt
     4321 aggttacgcc gtcaacctga taggttgttt tgaaaatggc ccgttccaaa tcgagttctc
     4381 gatagtaatt ggtgaagttt tcatgtcctg gaaaattcag gatcaaatcg ccaaccacct
     4441 gatattttga accatgtaat tgtgtcacca tgttctcgtt caccattgct tcggcttctt
     4501 tgaactttcc gctgaaaatc agttcccgaa ttttgggcaa agcttccaac gctttggggt
     4561 tgtcgtttcg agacggacca cccgaccaaa acgtttcctc gttcaactga actttttctg
     4621 tagcgggatc gccataaatc atggcaccca atcgaccgtt tccgacagga agagcttcgt
     4681 tccaaacctt tgcaggcttg tcgtaccaca tcttcagctt gcctccctgg ggttcgctgc
     4741 tttggcaggc tgcaaagcag aaaacaccca gcagcaccaa cattaatttc acttttctca
     4801 tttgtttatc aatcttttat ttcagaaatc aattttatta tcttttgagg tttacaaaaa
     4861 ggaaaccttc gttttttctc cttgataatc aacagatttt ctctgcaaat tttcacccgc
     4921 tttaacaagt acaacgttaa aaatcctttt tttcaacatt cccggaaacg aaccgctgcg
     4981 atcagcaacg gtcaacgttt ttttggcatc gtcccaagtg aattggatag tcgaaaactg
     5041 ccctttttcg tagttat
//
LOCUS       DAOPUW010000046         5050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_16651_length_5050_cov_3.964765, whole genome shotgun sequence.
ACCESSION   DAOPUW010000046 DAOPUW010000000
VERSION     DAOPUW010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16651_length_5050_cov_3.964765"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PKJ43_01420"
     CDS             complement(<1..816)
                     /locus_tag="PKJ43_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71246.1"
                     /translation="MKNYKTAVIFTGSGGTISQEVAILDQLIKTGKIELNEDNTLLIG
                     SGSGALNLVAVNACFRKQAPCSWDNFYKGIFLPSITDNEIFIKVDPIHWITLPQRKKF
                     AKLLNEAGFTTISDLPFDSIILTSVVNENKTKWMKSRAKKLDKLNLDDILMASSAVPV
                     LFPSHQLNSNADSLHSKFLGAHYEGSACGLLNKFKKQLGRIVLEHGTFEQVFIISPKR
                     LFDYSPVILHDLSMMIAQEKYQFNQFLNHISLHGFLTFLIKLQKNNSKNRLAKS"
     gene            complement(867..2708)
                     /locus_tag="PKJ43_01425"
     CDS             complement(867..2708)
                     /locus_tag="PKJ43_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNZ71247.1"
                     /translation="MRFRAQITLLFLVLVAAFTSIAVFTSVSFLKIKKFSEIDKMVYQ
                     LYNHSLEMKKNEGDYFNWDLKNPGYFRTGKSEQFEQFKKSYKLSEEICIQLENSSFIK
                     RNKFKGNIDEINQLLSKYNNLFSIIEKNKLELGFEEWGLLGQMNNSVTKLEREIESRN
                     DSQLKIQLLTLRQYEKDYLFRRDFNYKQLFDRKLFSIFEKLKSSGISETSTIFNLFKL
                     YGNNFNNLVDKDFYIGSTKEEGLVASLEMVSENLNKSITLLSQNIPQKTKLYINKTVS
                     FLIVFIAICTAGALLIGLFIFRRILSLLGGEPEEVALIANNIAKGNLQIKFDETKDYE
                     GVMKSMVTMTRKIGAIIQNIYKSSDQVSIASQQFSLTSQTISKGAYDQSAAIDEIVET
                     IRSINDNIGQNSSNALETEKISNVIKEKIEAIRLQSDLSLETNKIISQKIEMINSIAK
                     QTQILALNAAVEAARAGIHGRGFNVVAEEVKRLADDSSLAASEIIKLTHNSLDEAENV
                     SELIHGIIEPIQKSTRLAQMISVASQEQSSAALQINQTIQGLYHLSQENAVASEEMAA
                     STVELEQQIGSLKQMVEYFNIATDSSGRIIKIKAKKKKKKKAPHSAA"
     gene            complement(2717..3184)
                     /locus_tag="PKJ43_01430"
     CDS             complement(2717..3184)
                     /locus_tag="PKJ43_01430"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HNZ71248.1"
                     /translation="MRKFGFLLGMLLIMAISAFAQDIKEIDGIYFKGNTPYSGDFTIF
                     HENGVPKIELLLVEGMKEGAVKVYFENGTLSEIRSYKKNVMDGTWLTFNEKKVRIAEA
                     HYLDGKKDGKWYIWDDDGRLIYELEYTNGEKTGNWKKYDTAGNVISERNYSIK"
     gene            complement(3354..>5050)
                     /locus_tag="PKJ43_01435"
     CDS             complement(3354..>5050)
                     /locus_tag="PKJ43_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ71249.1"
                     /translation="MMLATNEEVKIADAFNYDTLNFMPASGSPVFNASIWAGNTSSDG
                     VSIENSFFEKVNFVGAFSELNNWTAGWTEWNPVEKEYPAATATKGNAQFSRSTGLHIT
                     TDETWSGVIKLDGWVYVDAGATLTIQPGTIVRGTEKSGIFIERGGKIMAVGTQSSPIV
                     FTSNQGAGLRSQSNWAGIVICGKAPNNLAGGEGIAEGGIDSPYGGTDPHDNSGVLKYV
                     RIEFPGYEVATGKEINGLTLCSVGDATTLEYIQVSYSGDDGFEWFGGTVNAKYIISYK
                     TEDDDFDTDNGFVGLVQYGLISRDPDIVDTDTANAFESDNDATGSTSAPFTQAVFSNV
                     SAFGPIASASTAALAKNHEEGAAMRIRRSSKLQIYNSLFMGWGRGVRLESDNTLTSAK
                     NGEMNINNTIIAGTRNDKYKVDGTVMDVAALEAWHLTTEKENMMLATNEEVKIADAFN
                     YDALNFMPLAGSKVLNASYWAEPVSTETIKSTSQKVYPNPFSGTTIIDIVTTEDTQLS
                     VNVYNSMGSKVALLYNGKVNAGKHSVIFDATSLPKGIYMGVIATEKETRSFKMVSR"
BASE COUNT         1267 a         1056 c         1077 g         1650 t
ORIGIN      
        1 ggactttgcc agcctgtttt tcgagttgtt tttttgcagt tttatgagga acgttaggaa
       61 accatgcagt gagatatggt tgaggaactg gttaaactgg tacttctcct gagcaatcat
      121 cattgaaaga tcgtgaagga tgaccgggct gtaatcgaaa aggcgttttg ggctgataat
      181 aaaaacctgt tcaaacgttc catgttcaag gacaattcgc cccaattgtt tcttgaactt
      241 atttaaaagc ccacatgctg acccttcgta gtgagcaccc agaaatttgg aatgaaggct
      301 atcggcgttg ctgttcaatt ggtgcgatgg gaacagaacg ggaactgccg atgaggccat
      361 aagaatatca tctaaattga gcttgtctag tttctttgcc ctgctcttca tccatttggt
      421 tttattctcg tttactaccg aagtcagaat tatcgaatcg aacggaagat cggagatggt
      481 tgtaaaacct gcttcattga gcagtttagc gaattttttc cgctggggca gggtgatcca
      541 atgtattgga tcgactttta tgaaaatctc attgtcggtt atcgatggaa ggaatattcc
      601 tttataaaaa ttatcccacg aacaaggggc ttgtttcctg aaacaggcat tcacggccac
      661 aaggtttaat gcgccagatc ccgatccgat aagtaaggta ttgtcctcgt taagttcaat
      721 ttttcctgtt ttaatgagtt gatcaaggat tgctacttcc tgagaaatag taccgcccga
      781 accagtgaaa ataacagctg ttttataatt tttcataaaa ttcaatgctt gatttggttt
      841 tttctaattg ttctttcttg ttaaggttat gccgctgaat ggggtgcttt tttcttcttt
      901 ttctttttgg ccttaatctt aattattctt ccactgctgt cggtggcaat gttgaagtat
      961 tcaaccatct gtttgagtga gccgatctgt tgttcaagct cgacagtgct tgcggccatc
     1021 tcttccgacg caacggcatt ttcctggctc agatggtaca gaccttgaat ggtctggttg
     1081 atttgtaatg cagcagagct ttgttcctgg cttgcaaccg aaatcatttg ggcaagtctt
     1141 gtcgattttt gaatgggttc gatgatccca tgaataagct cacttacatt ctcggcttca
     1201 tcgaggctgt tgtgagtaag ttttataatc tcgctggcag caaggctgct gtcgtcggcc
     1261 aatcgcttca cctcctcggc taccacattg aagccccgtc cgtgaattcc tgcacgtgca
     1321 gcttcaaccg ctgcattcaa cgccaatatc tgggtttgtt tggcaatcga attgatcatt
     1381 tcaattttct gcgaaatgat tttgttggtt tcgagcgaaa ggtcggactg taatcttatg
     1441 gcctcgattt tttctttaat aacattgctg attttctcgg tttcgagggc attggacgag
     1501 ttctgcccaa tattgtcatt tattgaccga atggtttcga caatttcatc gattgctgcc
     1561 gattggtcat aagctccttt agaaatggtt tgcgaggtta ggctgaattg ttggcttgct
     1621 attgaaacct ggtcactgct tttgtaaata ttctggatta tggctccaat tttccgggtc
     1681 attgtgacca tcgatttcat taccccttca taatctttag tctcatcaaa cttgatttga
     1741 aggtttccct ttgcaatatt attggcgata agagccactt cctcgggttc gccacccaaa
     1801 agactgagta tcctgcggaa gatgaataag ccaataagta aagcacctgc agtacaaatg
     1861 gcgataaaga caataagaaa ggaaactgtt ttgttgatat atagcttggt tttctgcgga
     1921 atattctggc ttagcagcgt gatcgacttg ttgaggtttt cgcttaccat ttcgagcgaa
     1981 gcaaccaacc cctcttcttt ggtggaaccg atataaaaat ctttatcgac caaattgttg
     2041 aaattgttcc cgtaaagctt gaacaggttg aagatggtcg atgtttctga aattcccgat
     2101 gatttgagtt tttcaaaaat agagaacaac tttcggtcga acaattgttt gtagttgaag
     2161 tcccttctga acaaatagtc tttttcgtat tggcgaaggg taaggagctg gatttttagc
     2221 tgtgaatcgt ttctcgattc aatttccctt tcaagtttgg tgaccgaatt gttcatttgc
     2281 ccaagcaaac cccactcttc gaacccaagt tcaagtttgt ttttctcgat tatagaaaag
     2341 aggttgttgt attttgaaag tagctggtta atctcgtcta tattgccttt gaatttattc
     2401 cttttgatga acgatgaatt ttcgagctga atacaaattt cttcggacag tttatagctt
     2461 tttttgaatt gttcgaattg ttcgcttttg cctgttctaa agtatccggg gtttttcaag
     2521 tcccagttga aataatcgcc ctcgttcttt ttcatctcaa gcgaatgatt gtaaagctgg
     2581 taaaccatct tgtcaatctc tgagaacttt ttgatcttga gaaacgagac agatgtaaac
     2641 acagcaattg aagtaaatgc agcaacaaga actaaaaaca aaagcgtaat ttgagccctg
     2701 aacctcataa ttaggtttat tttatagaat aattccgttc gctaattaca tttccggcag
     2761 tgtcgtattt cttccaattc ccggtttttt ccccgttggt atattccagt tcgtaaatta
     2821 gtcttccgtc gtcgtcccag atgtaccatt tgccatcttt ttttccgtcg aggtaatggg
     2881 cttcggcaat tcgtactttc ttttcgttga aggttagcca ggttccgtcc attacatttt
     2941 ttttgtagga acggatttcg ctcaacgttc cattttcaaa gtagactttt acggcccctt
     3001 ctttcatccc ctcaaccaac aacagttcaa ttttgggaac cccgttttcg tgaaaaatgg
     3061 taaagtcacc gctgtaaggt gtattgcctt tgaagtaaat cccatcaatc tctttaatat
     3121 cttgagcaaa agccgaaatt gccattatta gcagcattcc aagtaaaaag ccaaattttc
     3181 tcatacagtc actttctttt tgatttattt aaaattgatc attcgctaaa tagttgaaaa
     3241 tcaaatttca gattcggtcg ttgggcattt tttaataaga aggaatagtt ccgggttttt
     3301 accccggaac tatcttcttt tatattgaag agttttttta acattgaatc gtcttatcgt
     3361 gaaaccattt taaaagatct ggtttctttt tctgttgcaa taactcccat ataaatccct
     3421 tttgggagtg atgtagcatc gaaaattacg ctatgttttc cagcattgac tttaccattg
     3481 taaagcaatg ctactttcga tcccatgcta ttgtagacgt ttacagagag ttgtgtatct
     3541 tcagttgtca caatatcaat aattgttgtt ccgctaaacg ggttagggta aactttttgc
     3601 gatgttgatt taatcgtttc tgtagaaaca ggttctgccc aataagaagc atttaacact
     3661 tttgaaccag ccaaaggcat gaaattgagt gcatcgtaat tgaaagcatc tgcaatcttc
     3721 acttcttcgt tagtggcaag catcatgttt tctttttcag tggtgaggtg ccatgcttcc
     3781 aacgctgcaa catccatcac tgttccgtca actttgtact tgtcgttgcg agttccggca
     3841 atgatggtgt tgttgatgtt catttcaccg tttttagctg aagtgagcgt gttgtcagat
     3901 tcgagcctta caccacgacc ccagcccata aagagcgagt tgtagatttg gagcttcgag
     3961 ctacgacgaa tacgcattgc agcgccctct tcgtggttct tggctaaagc tgctgtcgaa
     4021 gcagaggcga tcgggccaaa cgcgctaaca ttgctgaaga cagcttgagt aaaaggagcc
     4081 gatgttgaac cagtggcatc gttgtccgat tcgaatgcat tggcagtgtc ggtatccaca
     4141 atgtcggggt cgcgtgagat aagcccgtac tgaacaagtc caacgaaccc gttgtcggta
     4201 tcaaaatcgt cgtcctcggt tttatatgag ataatgtatt ttgcatttac agtgccgcca
     4261 aaccattcga aaccgtcgtc gcccgagtag gaaacctgga tgtactctag cgttgtagca
     4321 tcgcctaccg agcaaagagt aaggccgttt atctctttac ctgtggccac ttcgtagcca
     4381 gggaattcga tgcgtacata tttcagtacc cctgagttgt cgtgaggatc agttccacca
     4441 taaggtgagt cgataccgcc ttcggcaata ccttcgccgc cagcgaggtt gtttggcgct
     4501 ttaccacaaa ttacgatacc tgcccagttg ctttggctgc gaagtcctgc accctggttc
     4561 gaagtgaaaa caataggtga tgactgtgtt cccactgcca tgatcttacc accccgttcg
     4621 atgaagatac cgctcttttc ggtaccacga acgatggttc ctggttgaat agtgagggtt
     4681 gcccctgcat cgacatacac ccagccgtcg agcttgatca ctccgctcca ggtttcgtca
     4741 gtagtgatgt gtaaccctgt gctgcgagag aactgcgcgt tacctttggt tgcagtggca
     4801 gcaggatatt ctttttcaac cgggttccat tcagtccaac cggctgtcca gttgtttaat
     4861 tcactgaagg ctccaacaaa gtttactttt tcgaaaaaag agttttcgat ggatacacca
     4921 tcagaagatg tgttgccagc ccatatagac gcattgaaaa caggtgaacc gcttgcaggc
     4981 ataaagttca aagtgtcgta attgaaagca tctgcaatct tcacttcttc gttagtggca
     5041 agcatcatgt
//
LOCUS       DAOPUW010000047         5039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_16696_length_5039_cov_3.141252, whole genome shotgun sequence.
ACCESSION   DAOPUW010000047 DAOPUW010000000
VERSION     DAOPUW010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16696_length_5039_cov_3.141252"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PKJ43_01440"
     CDS             <1..674
                     /locus_tag="PKJ43_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNZ71250.1"
                     /translation="NYNFMDFSDKNYIPLKTNYLILPEFGITYRAQFSRWFSVLPRLF
                     FFKLGEVVSQTENYTLGINDLGLLVPIDFQIPLKSKRKRGTTKIHMYAGPYGAIPLFA
                     NITTKKNSLRMSTNELTPWNYGIETGIGIRIPTFTLEASSNLNFRIAYRRGFSPLYQD
                     LNIKAGAPFADNDITLIGGNRFNQSISLVVGIEIPMKSKSFVSFTAGGDGKKNYKKVV
                     VIDDK"
     gene            complement(733..>1338)
                     /locus_tag="PKJ43_01445"
     CDS             complement(733..>1338)
                     /locus_tag="PKJ43_01445"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNZ71251.1"
                     /translation="RLYEMFMGPLDATKPWTETGVKGVYNFLKRVYYFFADTNNIVDG
                     EEDKETLKALHQTIKKVGDDVEDMKFNTAISQMMIFTNHCYKMKKVTRETAETFAKVI
                     ALFAPHLAEELWAIYGNEPSVSLLPWPSVNEKFLVEDVFAYPVSFNGKTRFNLELPVD
                     MPVAEVEKAALAHESSARWMEGKQVVKVVIVPKKIVNIVLK"
     assembly_gap    1339..1438
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1439..3207)
                     /locus_tag="PKJ43_01450"
     CDS             complement(<1439..3207)
                     /locus_tag="PKJ43_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNZ71252.1"
                     /translation="MEYNFSEFEKKWQKYWLDNKVFASEADTSKPKFYCLDMFPYPSG
                     AGLHVGHPEGYTATDIVCRYKRAKGFNVLHPMGWDAFGLPAEQYAIQTGTHPAITTQK
                     NCDTFRRQIQSLGLSYDWDREINTTDPAYFKWTQWIFIRLYNTWFDAELQKGRPISEL
                     PIPANIKQQGSEAVTRYIESKRLAYYDNAQVWWCNHCKTVCANEEVLNDGSHEKCGTK
                     EVERRFLKQWMLRIPLYAERLLQGLDGLDWPAGVKDMQRNWIGKSTGAEVDFEIDGLN
                     KKLKVYTTRPDTLFGATYMVIAPEHELVNVITTEAQKVDVENYIKVAALKSDLDRTEL
                     SKEKTGVFTGRYAINPLTGKQIPIWIADYVLTGYGTGAIMAVPAHDTRDFEFAKTFNL
                     PVVCILDPKEADDTLREKVLAGDACWTEDGAYINSANPVLGLNINGMSKQDGISTVIG
                     WLGDRGIGKATVNFKIRDWLFSRQRYWGEPFPIIHWEDGEISVVEDENLPVELPKTDK
                     YQPGDSGESPLANATEWLQVVDKNGRKGRRETNTMPQWAGSCWYYLRYIDPKNDKEPF
                     SKVKENYWMPVDLYVGGAEHAVL"
     gene            complement(3408..4097)
                     /locus_tag="PKJ43_01455"
     CDS             complement(3408..4097)
                     /locus_tag="PKJ43_01455"
                     /inference="COORDINATES: protein motif:HMM:NF039966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HNZ71253.1"
                     /translation="MKKTLKYIFSTLIILLSLTVSAQTASRNGLSLGVDLSRIAVPFI
                     DSTRYGWEALADIQIVPNVFVAAEVGSQTSRFSKPEYHYSSSGAYARLGLDYNFIKHL
                     DDKSEDKIFIGARLGFSSFYHDADNLLVRDSIWGDINPQPIPVKWLTSGWTEVALGMR
                     ARLINNFYLGWSVRAKIRMWLGKDERMHPFSIPGYGKANVKNTFGVNYSLYYMIPLRK
                     KTDVPKESGTQ"
     gene            complement(4069..4545)
                     /locus_tag="PKJ43_01460"
     CDS             complement(4069..4545)
                     /locus_tag="PKJ43_01460"
                     /inference="COORDINATES: protein motif:HMM:NF040109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6452 family protein"
                     /protein_id="HNZ71254.1"
                     /translation="MKIKIHTFLLILAVVLFVASCDPYSPCEDTDGVQANLGFYSFNG
                     KTLADTSLASLKIYAIDGDSSVFIREYTQKVNKIAVPLSMVADTSVVCITYGKGSPDT
                     LKFYYTQFTRLVSHECGFANFYTLTGIETTTHLIDSVKIRKYLVEYGDEENIKIYF"
     gene            4755..>5039
                     /locus_tag="PKJ43_01465"
     CDS             4755..>5039
                     /locus_tag="PKJ43_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ71255.1"
                     /translation="MIKVCALASGSNGNSYYIGNEQEAILVDAGINCKQLLLRMEERN
                     LDPQKVKAVFITHEHTDHICGVRVFCNKLSIPAYFTRGTFLNTSNRHKPRL"
BASE COUNT         1498 a         1099 c          940 g         1402 t
ORIGIN      
        1 gaaattataa cttcatggat tttagcgata aaaattacat tccactcaaa acaaactact
       61 taattcttcc tgagtttggg atcacttacc gagcccagtt tagccgatgg ttctcggtat
      121 tgccccgact tttctttttt aaattagggg aagttgtgag ccagacggag aattacacat
      181 taggaataaa cgatttagga cttttggtcc ccattgattt tcagattcca ctgaaatcaa
      241 agagaaaacg gggaacaact aaaatccata tgtatgcagg gccttacgga gccattcctc
      301 tcttcgcaaa tattacgaca aaaaagaatt cgttgcgaat gtctacaaat gaacttactc
      361 catggaatta tggaatcgaa accggcattg gaatcagaat tcccactttc actcttgaag
      421 caagcagtaa tctgaacttc aggattgctt accggagagg tttctctcct ctttatcaag
      481 acttaaatat aaaagccgga gccccatttg ccgacaatga tattacacta atcggtggca
      541 accgatttaa ccaatctatt tcgttggtag tgggcattga aattccgatg aaatcgaaga
      601 gtttcgtttc gtttactgcg ggtggagacg gtaagaagaa ttacaaaaaa gtagttgtaa
      661 tcgacgacaa ataaaacaac aaaaaggctg ttcctaatcg aaacagcctt tcttatattc
      721 atgtaagcat ttttatttca atacaatatt gacaattttc ttcggtacaa tcaccacttt
      781 gaccacttgt tttccttcca tccaccttgc agacgactca tgtgcaagtg cagctttttc
      841 aacttcggca acaggcatat caactggcaa ttccaaattg aaacgagtct ttccattgaa
      901 tgataccgga taggcaaaaa cgtcttccac aaggaatttt tcgttcactg atggccaagg
      961 caaaagactt acgctgggct cgtttccata aattgcccat aattcttcag ctaggtgcgg
     1021 ggcaaacagg gcaattactt ttgcaaaagt ttcggcagtt tcgcgcgtta cttttttcat
     1081 tttgtaacaa tgattggtaa aaatcatcat ttgcgaaata gccgtattga atttcatatc
     1141 ttcaacgtcg tcgcccacct ttttaatcgt ctgatgtaac gctttcaatg tttccttatc
     1201 ttcttcgcca tctacaatat tattagtgtc agcaaaaaag taataaaccc tcttcaagaa
     1261 attgtatacc cctttaacac cggtctcagt ccatggcttg gtagcatcga gcggccccat
     1321 aaacatttcg tacaagcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg
     1441 aagaacagca tgctcagctc cacccacata caaatcaacc ggcatccaat agttttcttt
     1501 tactttacta aaaggttcct tatcgttttt tgggtcgata tagcgaagat aataccagca
     1561 tgagcctgcc cattgcggca tggtattggt ttcgcgccgg cctttgcgac catttttatc
     1621 gactacctgt aaccattctg tagcgtttgc caacggcgat tcaccagaat ctccaggctg
     1681 atatttatcg gtctttggca attcaaccgg aagattttca tcctcaacaa ccgaaatttc
     1741 gccatcttcc caatgaataa taggaaatgg ttcgccccag taccgctgac ggctaaacaa
     1801 ccaatcgcgt attttaaaat taacggttgc tttacctatt cccctgtccc cgagccagcc
     1861 aatcacggtg gaaataccat cttgcttgct catgccatta atattaaggc ccagcaccgg
     1921 atttgctgaa ttgatatacg ctccgtcttc agtccagcaa gcatcaccag ccagaacttt
     1981 ttcccgaaga gtatcgtcgg cttccttagg atcgagaatg caaacaacgg gtagattgaa
     2041 tgttttggca aattcaaagt cgcgggtatc gtgggccgga actgccataa ttgccccggt
     2101 accataacct gtaagtacat aatcggcaat ccagattgga atttgctttc ctgtaagggg
     2161 attaatagca taacgacccg taaagacccc tgttttttcc ttcgaaagct cagtacgatc
     2221 gaggtcgctc ttcaacgctg ccacttttat gtaattttca acatcaacct tttgtgcttc
     2281 ggtggtaata acatttacca gttcatgctc gggtgcaata accatataag ttgccccaaa
     2341 aagagtatcg gggcgggtag tatacacttt aagcttttta ttcaagccat ctatttcgaa
     2401 gtcgacctcg gctccggtag atttaccgat ccagtttcgt tgcatatctt taactcctgc
     2461 gggccaatcc aacccatcaa ggccctgcaa gagacgttcg gcatacaatg gaatacgaag
     2521 catccattgc tttaagaacc gacgttctac ttctttcgtt ccacatttct catgagaccc
     2581 gtcgttaagt acttcttcat tggcacaaac tgttttacag tggttgcacc accatacttg
     2641 tgcattgtcg taatatgcaa gccttttcga ctcaatatag cgtgtaaccg cctcgctgcc
     2701 ctgttgctta atattggcag gtataggcag ttcgcttatt ggccgccctt tttgaagttc
     2761 ggcatcaaac caagtattat acaaacggat aaaaatccac tgcgtccatt tgaaataggc
     2821 cgggtcggtt gtatttatct cacgatccca atcatagctc aatccaagtg attgaatctg
     2881 acggcgaaag gtatcgcagt ttttctgagt agtaatagcc gggtgcgttc cggtttgaat
     2941 ggcatattgt tcggctggca aaccaaacgc gtcccacccc attggatgta gtacattaaa
     3001 cccttttgcc cgtttgtacc gacaaacaat atcggtggct gtgtaacctt cggggtgccc
     3061 tacatgcaac ccagctccag aaggataagg aaacatatcg agacagtaaa attttggttt
     3121 cgaagtatcg gcttcgctgg caaacacctt gttatcgagc cagtattttt gccacttctt
     3181 ttcaaactct gaaaaattat attccattac attaaaaata agggtttaac aatcaggggg
     3241 caaaaatagg aaaaagattg tgagtagagc cagaaaaaac agatcaaaag ctgaggtgcc
     3301 agattaaaat ttaatcatgg ttaacagatt attaaacgag agcctgtcta atgaccgaaa
     3361 agcacattcg ttaaaattta atcttgtaaa acaggagctt aaaaggatta ttgagtgcct
     3421 gactctttgg gaacatcagt tttctttctc aacggaatca tgtaatagag agaataatta
     3481 accccgaagg tgtttttcac attggctttc ccatatcccg gaatagaaaa tgggtgcatc
     3541 cgttcgtcct ttcctaacca cattcggatt ttcgcccgaa ccgaccatcc cagatagaaa
     3601 ttattgataa gccgggctct catgccaagc gcgacttctg tccatcccga tgtcaaccac
     3661 tttaccggta tgggctgagg gtttatgtct ccccaaatag aatcacgaac caggaggtta
     3721 tcggcatcat gataaaaaga agaaaagccg agccttgccc caataaatat tttatcctcg
     3781 cttttatcgt caaggtgctt tatgaaatta taatcaagac ctaaccgggc ataagcccct
     3841 gaagaggaat aatgatattc gggctttgaa aaacgtgagg tttgagaacc aacttcagca
     3901 gcaacaaaca cattgggtac tatctgaata tcagccaatg cttcccatcc gtagcgggta
     3961 gaatcgataa atggcacagc aatacgcgac aaatcaacac caagcgacaa accatttctg
     4021 gatgctgttt gggcacttac tgttagcgat aaaagaataa tcagcgtgct aaaaatatat
     4081 tttaatgttt tcttcatcgc catattcaac taagtacttt ctaattttaa ccgaatcgat
     4141 aagatgggtt gttgtttcta tccctgtgag ggtataaaaa tttgcaaaac cacactcgtg
     4201 cgaaacaagc cgtgtaaact gagtataata aaatttaaga gtatcggggc tcccttttcc
     4261 ataggtaata cacacaacag acgtatcggc aaccatcgat aaaggaacag caattttatt
     4321 gactttctgg gtatattctc gaataaagac agaactatca ccatcgatcg cataaatttt
     4381 gagcgaagca agagacgtgt cggcaagcgt tttcccattg aaactataaa aacccaagtt
     4441 tgcttgcacc ccatcggtat cttcgcaggg tgagtaaggg tcacatgagg caacaaacaa
     4501 tacaacagct aaaatcaaca aaaaagtatg tatcttaatt ttcatctact tctatttttt
     4561 tcctgaaaac gctacaaact tacaaaatag tctcaagtcg ataagatttt cacaccaaat
     4621 atgaaaaatt cacactccat caggcagatt accagcaaat atcgtttgtc taatttcaac
     4681 aagaaagaaa tgaagaacaa aatttgaaat aaagtaattt agagtgatta tttttggcgc
     4741 caaagaagaa agaaatgata aaagtttgtg ccttagcatc gggcagtaat ggcaacagtt
     4801 attatattgg caatgaacag gaagctattt tagttgacgc aggaatcaac tgcaaacagc
     4861 tattgctgcg tatggaagaa cgcaaccttg acccacaaaa agtaaaagct gttttcataa
     4921 cccatgaaca caccgaccat atttgcggag tgcgcgtatt ttgtaacaaa ttatctattc
     4981 ctgcctattt tacccgcggc acatttctga atacgtcaaa caggcataaa ccaaggctg
//
LOCUS       DAOPUW010000048         5006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_16834_length_5006_cov_3.538477, whole genome shotgun sequence.
ACCESSION   DAOPUW010000048 DAOPUW010000000
VERSION     DAOPUW010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16834_length_5006_cov_3.538477"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PKJ43_01470"
     CDS             complement(<1..773)
                     /locus_tag="PKJ43_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNZ71256.1"
                     /translation="MERLLRRLKGDKVIWIVLLILSLFSLLVVYSSTGSLAYRKAGGN
                     TFYFFARHFGMLLGGFFVILLVIKKVKIKYLSIFSSLSLLFSFGLLLLALVLRVGEGT
                     GRTLGIGFISFQPAELVKISLVLFVSRLLANNQDAENPPSAKTFISILAVSGVVCLGI
                     SLSNFSTAALLFLTIMFLMFIGRVPLKFLGITIAGGIAMVVLFYVLASSVNVGRINTV
                     KGRIDRFIKGDPESEVGITQADYAKMAIYNGGQAGKGPGG"
     gene            complement(775..2115)
                     /gene="murD"
                     /locus_tag="PKJ43_01475"
     CDS             complement(775..2115)
                     /gene="murD"
                     /locus_tag="PKJ43_01475"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNZ71257.1"
                     /translation="MNERLVILGAGESGVGSAILGIAKGFDVFVSDLGMPKDEYRQEM
                     ISRSISFEEGQHTEALILNADLVIKSPGIPEKAPIIKKLHEKAIPVISEIEFAGRYTS
                     AKTVCITGSNGKTTTTMLIYELLKHAGFKVGMAGNVGASFARMVAESDFEYYVLELSS
                     FQLDGMFDFKADVAVLMNITPDHLDRYNYELQNYVDSKFRIIQNQGANDVFIYFGDDP
                     IISTELQKRNIQSIQLPFTLGDPGPGNGARVSDGKIYVQFNEKNFEMSIHDLALQGKH
                     NTCNSMAAAIVGKVSDIRSEYIRECLSDFKGVEHRLERFLTVHGIEFINDSKATNINS
                     VWYALESMTRPVVWIVGGVDKGNDYSELFDVVKQKVKAIVCLGKSNEKIVEAFSNIVP
                     QIVETQSMEHAVKSAYFLAESGDVVLLSPACASFDLFNNYEDRGRQFKDVVRNL"
     gene            complement(2189..3442)
                     /gene="mraY"
                     /locus_tag="PKJ43_01480"
     CDS             complement(2189..3442)
                     /gene="mraY"
                     /locus_tag="PKJ43_01480"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNZ71258.1"
                     /translation="MLYHLYKLIEHWDIPGLGVMQYISFRASASVILSLIITMVFGKK
                     IIRLLQKKQIGEEIRNLGLEGQMQKKGTPTMGGVIIIASILVPTLLFARLDNIYTWLM
                     IITTVWLGFIGFVDDYIKVFRKDKKGLAGKFKVLGQVSLGILVASTLYFNDDVVVREK
                     VKDTNGNFISEIKVDAETGAQEVVYKSQHVKSTVTTLPFIKNQELDYRWLVKFLPDEW
                     EEVGSWLLYALIIIFIITLVSNGTNLTDGLDGLATGTTAISGLTLGILAYISGHVMLA
                     EYLNIMYIPNIGELAVFVAAFIGATVGFLWYNAFPAQVFMGDTGSLALGGILAVFSVI
                     IRKEIMIPLICGIFIIESLSVVMQVSWFKYTKRKYGEGRRIFLMSPLHHHYQKKGIPE
                     AKIVARFWIVAIMLAVISIVTLKVR"
     gene            complement(3453..4910)
                     /locus_tag="PKJ43_01485"
     CDS             complement(3453..4910)
                     /locus_tag="PKJ43_01485"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNZ71259.1"
                     /translation="MKRVKDIIEGINVLQVEGRLEAKVRGLAFDSRKVDEGFLFFAIK
                     GTHSDGHLFISDVIEKGATVVVCEELPSTLVGSVTYILVDDSAQALGLIASAFYDYPS
                     SKMKLVGVTGTNGKTTTATLLYNMVRNMGFKAGLISTIVYKVDDRTLPASHTTPDQLQ
                     LNSMMNEMVEAGCTYCFMEVSSHAIDQKRIAGLDFDGAIFSNITHDHLDYHKTFDAYI
                     RAKKQFFDGLKPDAFAITNIDDKNGNVMLQNTKASKISYSLRSMADHHCKIVESHFDG
                     TLLVIDGVEVWTHFVGKFNAYNLMAVYAAALALHFEREEVLNQLSLLFPVDGRFEVIR
                     SQNGTYAIVDYAHTPDALKNVLSTIDEIRTQKQSLICVVGAGGDRDKTKRPEMAAIAC
                     NISDKVILTSDNPRTEDPLMILKDMEAGVNEASKKKVLTIADRREAIKTAVMLALPGD
                     IILIAGKGHEPYQEIMGVKHHFDDREEVKRIFEIE"
BASE COUNT         1473 a         1172 c          938 g         1423 t
ORIGIN      
        1 ccaccaggac cttttccagc ctgccctccg ttataaattg ccatctttgc ataatcggcc
       61 tgggttatac ccacttcgga ttcgggatca cctttgataa accgatcgat acggccttta
      121 accgtattga ttcgaccaac attcaccgat gaggccaaca cgtaaaacag aacaaccatg
      181 gcaatacctc cggcaatggt aatgccgagg aacttgagtg gtacccgccc aataaacatt
      241 aagaacataa tggtcaagaa caacaatgct gcggttgaga aatttgacaa ggaaatacca
      301 aggcaaacaa ccccacttac agcaagaatt gatatgaatg ttttagccga cggaggattt
      361 tctgcatcct gattattggc cagcaggcgt gaaacaaaca atacaagcga tattttcacc
      421 aattcggccg gctgaaacga gataaaacca atccccaggg ttcggccagt tccttctcca
      481 acccgtaaaa caagggctaa aagcaacaat ccgaaagaga aaagcaggct tagtgacgaa
      541 aatattgaga ggtactttat ttttactttc ttaataacca acaatatgac aaaaaaacct
      601 cccagaagca ttccaaaatg acgggcaaaa aagtagaatg tatttccccc ggctttccga
      661 taggcaagcg aacctgtgga actataaacc acaagcagcg agaaaagaga taatatgagt
      721 aaaacaatcc atattacctt gtcgcctttt aatcttctga gaagtctttc catattataa
      781 gtttctgact acatctttaa attgccgccc ccgatcttcg taattgttga ataaatcgaa
      841 actggcacag gctggcgaca ataaaaccac atcgcccgat tcggcaagaa aatacgccga
      901 tttaacggca tgttccatgc tctgggtttc tacaatctgt ggaactatgt tgctaaatgc
      961 ctcaactatt ttttcgttgc tttttcccag acaaacaatg gccttcactt tctgtttgac
     1021 aacatcgaaa agctcactgt aatcatttcc tttatcaact ccacctacaa tccaaaccac
     1081 agggcgagtc atcgattcaa gtgcatacca aacagaatta atgttggtag ctttcgaatc
     1141 gttaatgaat tcgatcccat gaacggtaag gaaacgttcg agccgatgct caaccccctt
     1201 aaagtcggaa agacactcgc gaatgtactc gctcctgata tcagaaacct ttccaacaat
     1261 agctgcggcc atcgaattac aggtattatg cttgccttgc aaggctaaat cgtgaattga
     1321 catttcgaag tttttttcgt taaactgcac gtaaatttta ccatctgaga cccgggctcc
     1381 gtttccgggc cctgggtccc cgagggtaaa tggtaattga attgattgaa tatttctttt
     1441 ttgtaattca gtggaaataa tcggatcatc gccaaaatag atgaacacat cgttagctcc
     1501 ctgattttgt ataatcctga actttgagtc gacatagttt tgcaactcgt aattgtaacg
     1561 atcgagatga tcgggcgtaa tattcattag aacagccaca tcagctttaa aatcaaacat
     1621 accatcgagt tgaaagctgc tgagttcgag cacatagtac tcaaaatcag attcagcaac
     1681 catgcgcgca aaactagcgc ctacgttacc tgccatacca accttaaaac cagcatgttt
     1741 tagcaactca taaatgagca tagttgtggt tgtcttgcca ttgcttcccg taatacaaac
     1801 tgttttagcg gaagtatatc tcccggcaaa ttcaatttca gaaatcacag gaatggcctt
     1861 ttcatgtaat tttttgatta taggggcttt ttccggtatt cccggacttt taataaccag
     1921 gtcggcgtta agaataagcg cttcggtatg ttgcccttcc tcaaatgaaa ttgatctgga
     1981 aatcatttcc tgccgatatt catctttagg cattcccaaa tcggagacaa acacgtcgaa
     2041 tccttttgct attcccagaa tagccgatcc gactccgctt tcgcctgccc ccaatatgac
     2101 caatcgttcg ttcacttctt tatcttttat atatttcaga cagaatttca ggtttagtaa
     2161 aatgcataca gaaccgtcct tcttctgttt atctaacttt cagtgttaca atactaatca
     2221 cagccaacat tatggctacg atccaaaatc ttgcgacaat tttcgcttcg ggaattcctt
     2281 ttttctgata atgatgatgc aaaggcgaca taaggaaaat ccttcgccct tcgccatatt
     2341 ttcgtttcgt atatttaaac caagagacct gcattaccac cgaaagactt tcgatgataa
     2401 aaattccgca gataagagga atcatgattt cctttcggat aattacagag aatacggcaa
     2461 ggattccacc cagggcaaga ctccctgtat cgcccataaa tacttgtgcc gggaaagcat
     2521 tataccaaag aaaaccaacc gttgccccaa tgaaagcagc aacaaaaaca gccaactcac
     2581 caatattggg tatatacata atatttaagt actcggccag cataacgtga ccgcttatgt
     2641 aggccagaat tcccagagta agcccgctaa ttgcagtcgt tcctgttgcc aagccatcaa
     2701 gcccgtcggt aaggtttgtc ccgtttgaca ccaaggtaat tatgaaaatt ataatgaggg
     2761 catagagcaa ccaagagcca acttcttccc attcgtcggg aaggaatttc accagccacc
     2821 tataatcaag ttcctgattt ttaataaatg gaagcgtagt aactgttgat tttacatgct
     2881 gcgatttata tactacttct tgagctccag tctcagcgtc gacttttatt tctgaaatga
     2941 aatttccatt ggtatctttt actttttcgc gcacaaccac atcgtcgttg aaataaaggg
     3001 tacttgccac aagaatacct aacgaaactt gtccgagcac tttaaattta cctgcaagcc
     3061 cttttttatc ttttctgaat actttaatat aatcatcaac aaagccaatg aagccgagcc
     3121 aaacagtagt aataatcatc aaccatgtat atatgttatc gagcctggca aagagaaggg
     3181 ttggcacaag gattgaagca attatgataa ctccccccat cgttggcgtt cctttttttt
     3241 gcatttgccc ttcgagccca aggttcctga tttcttcacc aatttgcttt ttctggagca
     3301 agcgaataat tttcttacca aacaccatgg tgattatcaa cgaaagaata acagaggccg
     3361 aagccctgaa agaaatgtat tgcataactc caaggccggg tatgtcccaa tgttctatta
     3421 gcttatacaa gtgatacagc atcgcaattc atttattcga tttcaaatat tcttttcact
     3481 tcttccctgt cgtcgaaatg gtgttttacc cccattattt cctgataagg ttcatggcct
     3541 ttcccggcaa taagaataat atcaccagga agtgccagca taactgccgt ttttatggct
     3601 tctcgcctgt cggcaattgt gagtactttc tttttgctgg cctcatttac gccagcttcc
     3661 atatctttca atatcatcaa aggatcttca gtccggggat tatctgacgt aagtattact
     3721 ttatcgctga tgttacaggc aatagcagcc atctcgggtc gtttcgtttt atcgcggtct
     3781 ccacctgccc caacgacaca aatcaacgat tgcttttgag tgcggatctc gtcaattgtg
     3841 ctaaggacat ttttcaatgc atcgggcgta tgggcgtaat caacaatagc ataggtgcca
     3901 ttttgtgagc ggatcacttc aaatcgacca tcaacaggaa agagtaggct caattggttg
     3961 agtacctctt cgcgctcaaa atgaagggcc aaagcagcag cgtaaaccgc catgaggttg
     4021 taagcattga atttgcccac aaaatgagtc caaacctcaa ccccatcgat gacgagcaag
     4081 gtgccatcga aatggctttc aacaattttg caatgatggt cagccatcga acgaagagaa
     4141 taacttattt ttgacgcttt tgtattctga agcatcacgt tcccattttt atcgtcgatg
     4201 ttggttatgg caaaagcatc gggtttcaag ccatcgaaaa attgcttttt tgccctgata
     4261 taggcatcaa aagtcttatg ataatcgagg tgatcatgag tgatattcga gaatatagcc
     4321 ccatcgaaat ccaagccagc aattcgtttt tgatcaatgg catgcgaact aacttccatg
     4381 aaacaataag tacacccggc ttcgaccatc tcgttcatca tcgagttcaa ttggagctgg
     4441 tcgggagtgg tatggcttgc tggcaacgtg cggtcgtcaa ctttataaac aatggttgag
     4501 atcaatcctg ccttaaagcc catgtttctc accatattat ataaaagagt agctgtagtt
     4561 gttttaccat ttgtaccggt aaccccaacc aatttcattt ttgacgaagg ataatcgtag
     4621 aaagccgaag cgataagccc aagagcttgc gccgaatcgt cgaccaaaat ataggtcact
     4681 gaaccaacaa gcgttgaagg gagttcttca caaacaacaa ccgttgcgcc tttttcaatc
     4741 acatctgaaa taaaaagatg tccgtcgctg tgagtgccct ttatggcaaa gaagagaaag
     4801 ccttcgtcaa ctttccgcga atcgaaagcc agacctcgta ctttggcttc caagcggcct
     4861 tctacttgca ggacgtttat tccttctata atatctttta ctctcttcat attcactgtt
     4921 gctactttta acttagttca attaacaccc gctggcccct tttgcactgt gctccaggct
     4981 gaggcgattg cctgagtacc tttcct
//
LOCUS       DAOPUW010000049         4987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_16929_length_4987_cov_2.947891, whole genome shotgun sequence.
ACCESSION   DAOPUW010000049 DAOPUW010000000
VERSION     DAOPUW010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16929_length_4987_cov_2.947891"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PKJ43_01490"
     CDS             <1..336
                     /locus_tag="PKJ43_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71260.1"
                     /translation="FVPSKHYVAIPSKTKRILPAFPPIELYYWKDTSYQLGKIEVEQN
                     GGIVNIYDIEKSVCDSIKFRNKVGEGLSAEVLKNYLKRSDRNLDKLLKYARQLKIESI
                     LQQQLKMLL"
     gene            complement(826..2481)
                     /locus_tag="PKJ43_01495"
     CDS             complement(826..2481)
                     /locus_tag="PKJ43_01495"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ71261.1"
                     /translation="MGLPVNIEDLIHGKAVEWERLEFKKGWNPEEIIHSICAFANDLN
                     NWGGGYIIVGIEENNGQPILPPKGIVPNFLDSIQGEAMQLSHQVLPNYFPIMQPYVLN
                     AKHILVLWCPAGDNRPYTAPSSQGKNAQRYPYIRFGSRSIIAKGENLRRLNELAARVP
                     FDDRTNGSAKIDNFDLGLMQAFLQEVKSDLFEESKKLSMTDLSRNMLIAKGPDEDLLP
                     VNVGLLFFCKEPEQFFPRCWIELVWHKDGSGRNFSEKYFKGPLHIHLRAVLAFIQNNI
                     LVETVTKIPGIAEAQRVWNFPYDAIEEAISNAVYHKSYELGSPIEIQIWPDKMEILSY
                     PGPVPPVDAQILKQNKRIVARDYRNRRIGDFLKELHLTEGRGTGFPTIYRAMLENTSP
                     DPIFETDEQCTYFLTTLPARVSVQVSDQVGVHVYDTVNLLVFNTIDEIIAYCNAVESC
                     ASDQVSDQVSDQVSSIVSDHISVYANDILVNLKMKPMSKKSLLQVLGLSNHILNKQRH
                     IDPILEVGWIEYTIPDNPKDRNQKYKISPSGLKLLTLLKQAQE"
     gene            complement(2694..3884)
                     /locus_tag="PKJ43_01500"
     CDS             complement(2694..3884)
                     /locus_tag="PKJ43_01500"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNZ71262.1"
                     /translation="MEDQQITDNQLERWRLILGGDESDGTGYNLPEELAQIDMALTAL
                     YDFDRQKSFNYGVEKGRGGQEGSNPGVARWLGDIRKYFPASVVNVMQNDALKHPELKK
                     KLMFEPEILEKTIADVHLVATLMELGKLIPSKTKDTARRVVQNVVDDLMKKLEQKTIS
                     ALHGALDKSARNFRPKFSEMNWDSTIRRNMKNYLPEYKTIIPEVKVGYGRKSKKSLRD
                     IILCIDQSGSMGTSVVYSGIFGAVMASIPSVKTKMIVFDTQVADLTEELADPVDLLFG
                     VQLGGGTDINLALGYCQELVSKPDDTILLLITDLYEGSDPQRMLQRIADLTNSGAQVI
                     CLLALNDDGCPAYDHKNAGELSKLGIPVFACTPYLFPDLMSAAIKKQDLKQWAGDNDI
                     VLKG"
     gene            complement(3896..>4987)
                     /locus_tag="PKJ43_01505"
     CDS             complement(3896..>4987)
                     /locus_tag="PKJ43_01505"
                     /inference="COORDINATES: protein motif:HMM:NF040143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HNZ71263.1"
                     /translation="LRLKLTAEPKQYDLDLRKELELQRSIFFFRLEILRIKWAKRTHS
                     RTKGTFKESWITEWSPDMMIDLIDKAFLGNTVQIAAQSIIMGECEAAQTVTGVTDLIV
                     LTIPSELFDCIDALLEKIVALSSISSDIIDLMKSLPNLIEVSRYGNVRKSDLTILNTI
                     VKQLLVKVFVGLPNACYGLDEDNSNQVFLLISRLNDVIRLYDDDDTLQQWNETLHHLI
                     DKDGVHPIILGCICRLLLDAKELSEQETDNRISFALSPANDPYRVASWLEGFLKGNGM
                     ILIYDNRLWNLIYSWVSLLKREVFMEVLPLLRRTFSTFEFGERKQIGLKAKQGIVKEN
                     IKVQSFDDEDFNGELAALVLPVVQQLGGF"
BASE COUNT         1441 a         1108 c          956 g         1482 t
ORIGIN      
        1 tttgttccct ccaaacatta tgtggctatt ccttccaaaa cgaaaagaat actgcctgct
       61 tttccaccca ttgagcttta ttattggaaa gacacatcgt accaactggg caagattgaa
      121 gttgaacaga acggaggtat tgtgaatata tacgatattg aaaaatcggt ttgtgattct
      181 ataaaattcc gcaacaaagt gggtgaagga ctatcggctg aggtgttgaa aaattatctt
      241 aaacgcagtg accggaacct tgataaacta ttgaaatatg cccggcagct taaaattgaa
      301 tctatcctgc aacaacaatt gaaaatgcta ctatgaagag ttttatcaag actcacccaa
      361 cctttttttt agtgttcggt cgaacttcca agctcgtttc attacgtcat ctcctttaat
      421 ccgttgactg aagcacaacg tctaaggata atttataccg attgaagaaa tgtaacagtc
      481 caatcttcgc ccactcgatt gtactgaaca tttctattca tcggcattat acatgaatag
      541 gttcggactg atttaaaatt gcatctggta ttatttcctt atttgtcttt tgaaccagag
      601 ggaaacgata cgagattaaa cattgcccac atccgtgacc gaaccggttg ccgtagcagg
      661 tttcgatttc gctggcggaa aggttggtgg tgatgaaaaa acatttgtcg acaaattttc
      721 ccgttaaaaa tcgtttgatt tcatacttaa caatttcata ttcaggaaaa tgatctctgg
      781 aaatttgatg ggatacatta ttgtttcagg cataacagct cttcgtcatt cctgtgcttg
      841 tttaagcaaa gtcaataatt tcaaaccaga tggagaaatt ttgtatttct ggtttctgtc
      901 ttttggatta tccggaatgg tatattcaat ccaaccaact tccagtattg gatcaatatg
      961 tcgttgttta ttaagtatat gattgcttaa acctagaact tgaagcagac ttttctttga
     1021 cattggtttc atctttaggt tcacaagaat atcattagca tatacactta tatgatcaga
     1081 aacaatacta cttacttgat cacttacttg atcacttact tgatcactag cacaactttc
     1141 aacagcatta caataagcaa taatttcatc aattgtatta aatactaata gattaacagt
     1201 atcatataca tgaacaccta cttgatcact tacttggaca cttacccgtg ctggaagcgt
     1261 agttaaaaag taggtacatt gttcgtcggt ttcgaaaatg ggatcaggag aggtgttttc
     1321 gagcattgct ctgtatatgg ttgggaatcc ggttccccgg ccttcggtaa ggtgcagctc
     1381 ctttaagaaa tcaccaattc ttcggtttcg ataatcacgg gccacaatgc gcttattctg
     1441 ctttaagatt tgcgcatcga caggtggtac cggaccagga taactgagta tttccatttt
     1501 atcgggccaa atttgaattt cgattggtga acctaattca tagcttttgt gataaacggc
     1561 attcgatatt gcttcctcaa tggcatcgta aggaaagttc caaactcttt gggcttcagc
     1621 aattcctgga atcttagtaa ctgtctcaac aagaatatta ttttgaataa aagctaatac
     1681 tgcccgtaaa tgaatgtgca aggggccttt gaaatacttt tcagaaaaat ttctaccgga
     1741 accgtctttg tgccaaacta gttcgatcca acacctggga aagaattgtt caggctcctt
     1801 acagaagaaa agtaagccaa catttaccgg aagtaaatct tcgtcaggac ctttagcgat
     1861 tagcatattt ctggaaagat cggtcatgga tagcttttta ctttcttcga acagatcgct
     1921 tttgacctcc tgtaaaaaag cctgcattaa cccaagatcg aaattatcga ttttagccga
     1981 gccatttgtc ctatcatcga aaggtacacg ggcagctaat tcatttaacc ttctaaggtt
     2041 ttcacctttt gcaataatgc ttcttgaacc aaaacgtatg tatggataac gttgtgcgtt
     2101 tttaccttgc gaactaggtg cagtataagg tcggttatcg ccagcggggc accataaaac
     2161 caaaatatgt tttgcattta acacataagg ttgcatgatg gggaaataat tgggcagtac
     2221 ctgatgagaa agctgcattg cttcaccctg aatactatcc aaaaagtttg gaacaatacc
     2281 tttggggggc aaaatgggct gaccgttgtt ttcttcaatg ccaacaatga tataaccacc
     2341 gccccagttg ttaaggtcgt tagcaaatgc gcatattgaa tggatgattt cttcagggtt
     2401 ccatcccttt ttaaattcga ggcgttccca ttcgacggcc ttgccatgaa ttaaatcttc
     2461 aatattaaca ggtaatccca tgcttttagt aaaaaagtaa aaataacaaa aaataggtgg
     2521 aagttgaatt ccaataaaac ttaacccgct tgttctttga atctttatcg aaagcaatca
     2581 cgttaaacat tgcccacatc cgtgaccgaa cccggttgcc atagaaggtt tcgatttcgc
     2641 gaacatatca attgggttat gccaaccacc ttatattgca caatttttta gccctaccct
     2701 ttcagcacaa tgtcgttatc gccagcccac tgttttaagt cctgtttttt aatggcagct
     2761 gacatcagat cgggaaaaag atagggggta cacgcaaaga ccggaatgcc aagtttcgat
     2821 aactcccctg catttttgtg gtcatacgcc ggacagccat cgtcgtttag tgccagaagg
     2881 caaataacct gagcccctga gtttgtcaga tcggcaattc gttgcaacat ccgctgggga
     2941 tcgctcccct cgtataagtc ggtaatcagt aacaaaatcg tatcatcggg cttggaaacc
     3001 agctcctggc aatatcccag ggcgaggtta atatcggttc ccccacctaa ttgcacccca
     3061 aaaagtaagt caaccgggtc ggccagctct tcggtcaaat cggcaacctg ggtatcaaag
     3121 acaatcattt tcgtttttac tgagggtatt gaagccatta cagcgccaaa aatgcctgaa
     3181 taaaccaccg aagtccccat cgatccactc tggtcgatgc aaagaatgat gtctctcaac
     3241 gatttcttcg actttcggcc atacccaacc ttaacttcag gaataatggt tttgtattcg
     3301 ggcaggtagt tcttcatgtt ccggcggatg gtcgaatccc aattcatttc gctgaatttc
     3361 ggacgaaagt tccgtgccga tttatctaac gcaccatgca atgcggaaat ggttttttgt
     3421 tccaatttct tcatcagatc atcaaccaca ttttgaacta cacggcgcgc ggtatctttt
     3481 gtttttgaag gaataagttt ccccaattcc atcagggttg caaccagatg gacatcagca
     3541 atagttttct ccaatatttc aggttcgaac atgagcttct ttttcaattc gggatgtttt
     3601 aaggcgtcgt tctgcattac attcaccacc gatgcaggaa agtatttgcg aatatcgccc
     3661 aaccaccgcg caaccccagg gtttgaccct tcctgccctc cccgtccttt ttctacgcca
     3721 taattgaacg atttttgccg gtcgaaatcg tacaacgccg ttaatgccat atctatttgc
     3781 gccagctctt ccggcaggtt gtagcctgtt ccgtccgatt cgtcaccgcc caatatcagt
     3841 cgccaccgtt caagttgatt atctgtaatt tgttgatctt ccatagttaa ctgaattaaa
     3901 acccgcccaa ttgttgaacc accggcaaaa ccaatgcagc taattcgcca ttaaaatctt
     3961 cgtcatcaaa agattgaacc ttaatgtttt ctttgacaat gccttgcttt gctttcaacc
     4021 caatttgttt acgttcacca aattcgaagg ttgagaaagt acggcgcaac aagggcaaaa
     4081 cttccataaa aacctcacgt tttaacaacg aaacccatga ataaatcagg ttccaaagcc
     4141 ggttatcgta aataaggatc ataccgttac ccttcagaaa accttcgagc catgatgcta
     4201 cccgatacgg gtcgtttgct ggcgacaaag caaaagaaat acggttgtcg gtttcctgtt
     4261 cagaaagttc cttggcatcg agcaacaaac ggcatataca tcccagaatt attggatgca
     4321 cgccatcctt gtcgataagg tggtgcaagg tttcgttcca ttgctgaagg gtatcgtcgt
     4381 cgtcgtaaag cctgattaca tcattcagcc tggaaatcaa taagaagact tgatttgaat
     4441 tatcctcgtc caatccataa caggcattgg gcaaacccac aaaaactttc accaggagtt
     4501 gctttacaat ggtgttcagt atggtcaaat cagatttgcg cacattccca taacggctaa
     4561 cctcaatcag gttgggcaac gatttcatca agtcgataat atcggacgag atcgacgaga
     4621 gggcaacaat tttttccaat aaagcgtcga tacaatcaaa caactccgaa ggaatagtaa
     4681 gaacgatcaa atcggtaacc ccggtaacag tctgtgccgc ttcacattcc cccataatga
     4741 tcgattgcgc tgcaatttga acagtatttc caagaaaggc tttatcgatc aagtcgatca
     4801 tcatgtcggg cgaccactct gttatccacg attctttaaa cgttcctttt gttcgtgaat
     4861 gggttcgctt tgcccatttt attctcaata tttctaagcg gaagaaaaag atgctccgct
     4921 gtaactccaa ttcctttctc aaatccaaat catactgctt aggttcagca gtcaatttca
     4981 aacgcag
//
LOCUS       DAOPUW010000050         4978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_16968_length_4978_cov_3.716433, whole genome shotgun sequence.
ACCESSION   DAOPUW010000050 DAOPUW010000000
VERSION     DAOPUW010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16968_length_4978_cov_3.716433"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /gene="ribB"
                     /locus_tag="PKJ43_01510"
     CDS             complement(<1..604)
                     /gene="ribB"
                     /locus_tag="PKJ43_01510"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HNZ71264.1"
                     /translation="MSGNQHEDFRNKLNTIQEAIEEIKNGKIIIVVDDEDRENEGDFI
                     AAAEKVTPEMINFMSKYGRGLICAPLTEKRCEELELHPMVGKNTSVHETAFTVSVDAL
                     GHGTSTGISAYDRSMTIKMLSDPSTKPEDLGRPGHIFPLIAKNKGVLRRSGHTEAAVD
                     LARMAGLQPAGILVEIMNDDGTMARLPQLLVIAKKFDLKIV"
     gene            complement(570..1748)
                     /locus_tag="PKJ43_01515"
     CDS             complement(570..1748)
                     /locus_tag="PKJ43_01515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ71265.1"
                     /translation="MIATLVIFWLCVWAVAHTYVIYPVLLHILLKIKGRKSFRSFDAG
                     DELPMVTILMAAYNEESVIEDKIRSVFNTSYPMQKVEMLVGSDNSTDNTNTIVRSLCE
                     EFPNLHFFNFEERQGKIGIINQLSDKSLGTILVVTDANVMFSVETLPQLIKYFADENV
                     GLVDTRMNNYGLKKDGISVQEKSYISREVYVKHLESSTFGTMIGPFGGCYAVRKSLFV
                     KVPSNYLVDDFFVCMSVFDQGKLAINNLDAPVYEGVSNNLSIEFKRKIRIAAGDFQNL
                     NHFKHLLWPPFSPVAFCFLSHKVMRWIGPFFLIGALFSNAILAVHSPFYQVLFLLQIT
                     LSFLPFVDFILKKIGIHIVLLRFVTHFYSMNLSLLIGFFKSLKGIKSNVWKPTRRLQE
                     "
     gene            complement(1745..2941)
                     /locus_tag="PKJ43_01520"
     CDS             complement(1745..2941)
                     /locus_tag="PKJ43_01520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNZ71266.1"
                     /translation="MKLLFLTNKPPYPPKDGGAIATLAMINAFARAGHETTVLAMNTR
                     KHHVTPWDLSPDLTNQTILHLVEVPAQITAKGAIFNFFFSKLPYNAERFIDKKYQNKL
                     LTLLETHSYDIIQIEGLYLLPYVPAIRKVTDCVIAYRSHNIEHEIWQRTTANAKRLRK
                     FYLQLLTRRMMRFEHEVVNQYDLVVPITHRDALKLDEMGNTKPSFVVPAGVDTALKTA
                     VDPFEMNIFYIGALDWTPNQDGILWFVKKCFSLVLKGMPRVRLKIAGRNAPDWLKKEL
                     VHPNIDFLGEIDDAGTYMAENGVMIVPLFSGSGMRVKIIEGMARAKAIVTTPIGCEGI
                     DAENGKMIYIADDAPLFAKYLLSLLSNRKKVETMGLEAARFVNRYYNNDELIANLTQF
                     YNKHLK"
     gene            complement(2916..4403)
                     /locus_tag="PKJ43_01525"
     CDS             complement(2916..4403)
                     /locus_tag="PKJ43_01525"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNZ71267.1"
                     /translation="MIKRFIGPFFLTFFISIFVLLMQFLWRYLDELVGKGLETSIIVE
                     LMSYASLSLVPMALPLAVLIASVMTFGNLGERSELIAIKASGVSLLQIMKPLIYLTSL
                     ITIVAYLLANEVIPVTNAKFTALLWSIREQRPEMIIKEGAFSNEIDGYSIKVNRRDDD
                     SELLYGIMIYDHTEDRGNVAVTIADSGYLRISDNKQYMILTLYNGESYNDEKPSGREK
                     RTSHPFRREKFSKQQIVISVKDYSLERADESIFKDGYRMLKNAQIASVVDSLTKLYVE
                     TEKSTTFSGNYNSELNEKIKDHFRVDSLKASTPADLKSVSVEFDQIFTSLDNDKKAMI
                     LEDAKRLAQSNQRNILQNEPILYNKIKWINRHIIEWHRKYTMSLACLIFFFIGAPLGA
                     IIRKGGFGTPVVVSILMFIFYYIVSMIGENFAREGVWEPTLTMWFSTFFFLIISIFMT
                     YQAVADTLLVSASVNSKILKRFKFLKSFLNSKEEPDQNETSVFDQ"
     gene            complement(4530..4901)
                     /locus_tag="PKJ43_01530"
     CDS             complement(4530..4901)
                     /locus_tag="PKJ43_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010421288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HNZ71268.1"
                     /translation="MAKVKFDMEYIFNCSPKVLYYRLNTASGLAEWFADDVDVNGKKF
                     TFYWDKAGREAILSFMKENLIVRYNWVDDGTFFEFRITRDELTGDVSLLITDFAEPDE
                     LPESRSLWDSQVALLKHVVGS"
BASE COUNT         1501 a         1083 c          972 g         1422 t
ORIGIN      
        1 aaactatttt caaatcgaat tttttggcaa tgaccaacag ctgaggcaag cgcgccatgg
       61 taccgtcgtc gttcataatc tcaaccagga taccggctgg ttgcaagcct gccattcggg
      121 ccaggtctac agcggcttcg gtgtgccccg agcggcgaag tacgcctttg tttttagcta
      181 tcagcgggaa aatatgcccg ggacggccaa ggtcttcggg tttggttgac gggtcagaca
      241 acattttaat ggtcatgcta cggtcatagg ccgaaattcc tgtggatgta ccatgtccca
      301 acgcatcaac cgaaaccgta aaagctgttt cgtgaaccga ggtatttttt ccaaccattg
      361 gatgcaattc gagttcttcg caacgttttt cggttagcgg ggcacaaatg agcccccgtc
      421 cgtatttcga catgaaatta atcatctcgg gcgttacttt ttcggcagct gcaataaagt
      481 cgccttcatt ttcccggtct tcgtcatcaa caacaataat gattttaccg ttctttattt
      541 cttcaattgc ttcctgaatg gtatttaatt tattcctgaa gtcttcgtgt tggtttccag
      601 acatttgatt ttattccttt taacgattta aaaaaaccta tcaacagaga aaggttcatc
      661 gaataaaaat gagttacaaa gcgcaaaagt acaatatgaa ttcctatttt tttcagtatg
      721 aagtcaacaa agggcaaaaa ggacagtgtt atttgaagta aaaataatac ttggtaaaag
      781 ggagaatgaa cggcaaggat tgcatttgag aaaagggcac caataagaaa gaatggacca
      841 atccatcgca ttaccttgtg agacaaaaag cagaatgcca ctggactaaa tggaggccac
      901 aacaaatgct tgaaatgatt taggttctga aaatcgccgg cagcaattct tatttttcgt
      961 ttaaattcaa tagaaagatt attcgaaaca ccttcataga caggtgcatc gagattatta
     1021 attgccaact ttccctgatc gaaaacactc atacaaacaa agaaatcatc gaccaaataa
     1081 ttcgatggaa ccttaacaaa caagcttttt cgtaccgcat aacaccctcc aaacggacct
     1141 atcatggtac caaaagtgct gctttcgaga tgcttaacat acacttcacg cgaaatatat
     1201 gacttttctt gtacggaaat cccatctttt ttcaagccat agttgttcat ccgtgtatcg
     1261 acaagcccta cgttctcatc ggcaaaatat ttaatcaatt ggggaagggt ttcaacagaa
     1321 aacatcacat tcgcatcggt gacaacaagt attgtcccca gtgatttatc tgacaattga
     1381 ttaatgattc cgatcttccc ctgtcgctct tcaaaattga agaaatggag attgggaaac
     1441 tcttcacaaa gggagcgcac aatcgtattt gtattatcgg ttgaattgtc gctacctacc
     1501 aacatttcaa ctttttgcat cgggtacgag gtgttgaata ccgaccgtat tttgtcttcg
     1561 attaccgatt cttcgttata ggctgccatt aagattgtta ccataggcag ctcgtcgcca
     1621 gcgtcaaaac ttcggaatga ttttcgtccc tttatcttaa gcagaatatg gagtaatacc
     1681 gggtatatga catacgtgtg agccacggcc cacacgcaca accagaatat taccaacgtt
     1741 gcaatcattt caaatgttta ttataaaact gtgtaagatt agcaatcagc tcatcgttgt
     1801 tataataacg gtttacaaag cgggcagcct cgagccccat tgtttcaacc tttttcctgt
     1861 tcgaaagcaa gctaagcaaa tatttagcaa aaaggggagc atcgtcggca atataaatca
     1921 ttttcccgtt ttcagcatct attccttcac agccaatagg tgttgttact atcgctttag
     1981 ccctggccat tccctcaatt attttcactc tcattccact tccggagaaa agagggacaa
     2041 tcatcacccc gttttcagcc atatatgttc ccgcatcatc aatctctccc aggaagtcga
     2101 tgttgggatg aaccagttct tttttcagcc aatcaggggc atttcgtccg gcaattttga
     2161 gccgaacacg cggcattccc tttaaaacca aagaaaaaca tttctttaca aaccacaaaa
     2221 ttccgtcttg atttggggtc cagtccaggg cgccaatgta gaagatattc atttcgaaag
     2281 ggtcaacagc cgttttcagc gcagtatcga cccctgcggg caccacaaag gagggttttg
     2341 tgttccccat ttcatccaac ttgagagcat cgcgatgagt tatcggaact accaaatcgt
     2401 attgatttac aacctcgtgt tcaaacctca tcatccgccg cgtcagtagc tgaagataaa
     2461 acttccttag ccttttggca tttgctgtag tgcgctgcca tatttcatgc tcaatattgt
     2521 gacttctata tgcgatgaca caatccgtaa cctttcgaat agcaggcaca tatggcagaa
     2581 ggtataagcc ttcaatctga attatgtcgt aggaatgtgt ttcgagcaac gtcaataact
     2641 tgttttggta ctttttgtcg ataaagcgtt ctgcattata tggcaatttt gaaaaaaaga
     2701 aattgaaaat tgctcctttg gcggtaatct gtgccggcac ctcgaccaaa tgaagtatgg
     2761 tttgatttgt caaatcggga gaaagatccc agggcgtaac atggtgttta cgggtgttca
     2821 ttgccaggac ggtagtttcg tggccagcac gcgcaaaggc attgatcatt gccagggtag
     2881 caatggctcc tccgtcttta ggcggataag gtggtttatt ggtcaaaaac agaagtttca
     2941 ttttgatcag gttcctcctt agaatttaaa aaactcttta aaaatttaaa tctcttcaat
     3001 attttactgt taacactggc cgagaccaac aacgtgtcgg ccactgcctg ataagtcata
     3061 aaaatactaa taatcaaaaa gaagaaagtc gaaaaccaca ttgtaagggt aggttcccaa
     3121 acaccttcgc gagcaaagtt ttcgccaatc atagatacaa tataataaaa aataaacatt
     3181 agaatagaca ctaccaccgg ggttccgaac cctccttttc gaataattgc acccaatgga
     3241 gctccgataa aaaagaaaat cagacaggcc agtgacatgg tatacttgcg gtgccattcg
     3301 atgatgtgcc ggttaatcca tttgattttg ttatataaaa taggctcgtt ctgaagaata
     3361 tttcgctggt tcgactgagc taagcgctta gcatcctcga gtatcattgc ttttttgtcg
     3421 ttgtcgagag aagtgaatat ctggtcgaat tcgacagata ccgacttgag gtcggccggg
     3481 gtactagctt tcagcgaatc aaccctgaaa tgatctttaa ttttttcgtt aagctcacta
     3541 ttgtagtttc cactaaaagt tgtagacttc tcagtttcaa catatagttt tgttaatgaa
     3601 tcgactacgg aagctatttg ggcgtttttc aacatccggt agccatcctt gaaaatgctt
     3661 tcatcggctc gctcaaggga gtaatcttta acactgatta caatttgctg cttcgagaat
     3721 ttttccctcc ggaaaggatg tgacgttcgt ttttcgcgcc ctgaaggttt ttcatcgtta
     3781 taactctctc cattatagag cgttaatatc atatactgtt tattgtcgga aatgcgcaaa
     3841 tagcctgaat cggcaatagt aaccgccaca tttccccgat cctcggtatg gtcgtaaatc
     3901 attattccat ataagagttc tgagtcatcg tcgcgacggt ttacctttat gctatatcca
     3961 tcaatttcgt tcgaaaaagc accttcttta atgatcattt ccgggcgctg ttcacgaatg
     4021 ctccaaagca gggcagtaaa ttttgcatta gtgaccggga taacttcatt ggccagcagg
     4081 taagccacaa tggtgattaa acttgtgaga taaatgagag gtttcattat ttgtagcaac
     4141 gaaaccccac tggcttttat ggcaataagt tcggaacgtt cacctaggtt tccgaatgtc
     4201 ataaccgaag caattagcac agctaagggc agtgccatag gaacaaggct aagcgaagcg
     4261 tacgacatca attcaacaat aattgacgtt tcgaggcctt tacccaccag ttcgtcgagg
     4321 tacctccaaa gaaattgcat cagcaacacg aagatgctaa taaagaaagt cagaaagaaa
     4381 ggacctatga acctttttat caaatataaa tatagtcgct tcataaaaac cattcgatga
     4441 atttacaaag ctaaaaatta gcttcgatta acgttgagaa tatagaaaag aaaagagtta
     4501 aaaagggtga aagagccagg cgctcaaacc tacgacccca caacatgctt caataaagcc
     4561 acttgagaat cccacaacga acgagactca ggcaactcgt cgggttcggc aaaatcggta
     4621 atcaacaaag aaacatcacc ggtaagttca tcacgcgtaa tccggaattc gaaaaaagtg
     4681 ccatcatcga cccaattata cctgacaatt aagttttctt tcatgaagct aaggattgct
     4741 tcacgccctg ctttatccca ataaaaagta aactttttcc catttacatc gacatcgtcg
     4801 gcaaaccact cggccaaacc cgatgcagta ttaagcctgt aatacagcac cttgggcgaa
     4861 caattgaaaa tatattccat atcgaactta actttggcca taggatttat ttaatctatt
     4921 gaacgcaata tatcaattaa gttttaattt accaatcatt gatacaggct aaaaaaag
//
LOCUS       DAOPUW010000051         4872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_17473_length_4872_cov_3.696076, whole genome shotgun sequence.
ACCESSION   DAOPUW010000051 DAOPUW010000000
VERSION     DAOPUW010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17473_length_4872_cov_3.696076"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1739
                     /locus_tag="PKJ43_01535"
     CDS             <1..1739
                     /locus_tag="PKJ43_01535"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNZ71269.1"
                     /translation="PQPQKPRYVEYQPFFNQLDKSFEGNKYAKNELDTVVKILSNLRD
                     QVYPITTTISFTDEFELSKGELLIDIELINEKPPLYDTFMVVKDFLMSEELMNTLFPL
                     LNDTTLEKSTPIDTLKKIETLPEEKPFTPTKATKSTKNKPKKITRRGSNPYKTINPKD
                     KRYSSKKKYYYPKQAEKPKKAPEPIKPAEPEKLITEAQDSIVKKSRKFYTRQEILSDT
                     TRDLLDADTLITNIFATTGWQVGDSIPFFAEYSDYTPDSFMQLVTDMVFVERYDPSRE
                     VKQYPKEELTREVATKKTENEKFVYNDKVVFRKFFKDEEDVFITMVKVKGDRFKIGNN
                     EFDEDERPEYGIQVSDFLIGKYETTNEVVCSFLNMLKCDEKGYRERTRVYNTNSEFSK
                     IYWDNKIGRFKVIKGFEKFPAVNITWTGANLIGKTGGGRLPTEAEWEFAAKGGRYAIK
                     YHTNSERTDYAYEHRFAGSNTMANVGWFVDNSGGYYHPVGELMPNLLGIYDMCGNVWE
                     WCFDNYDKEFYKRNGDSTDPFCVNNTSLRSNRGGSWSSDAQYCRITNRNFLDESQCNQ
                     FLGFRILKEWNR"
     gene            complement(1720..2199)
                     /gene="ybaK"
                     /locus_tag="PKJ43_01540"
     CDS             complement(1720..2199)
                     /gene="ybaK"
                     /locus_tag="PKJ43_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HNZ71270.1"
                     /translation="MKKTNAVRILDQHNISYELIEYEVDESDLSAIAVAKKLGQDIEQ
                     VFKTLVLRGDKSGIFVVVIPGNSEVDLKKAAKASGNKNAEMIAMKELLPLTGYIRGGC
                     TAIGMKKAYPNFIDESCLLFDFIYISAGVRGMQIKIAPEDLISLTHMTVDDFTCSIL"
     assembly_gap    2200..2299
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2520..3782
                     /locus_tag="PKJ43_01545"
     CDS             2520..3782
                     /locus_tag="PKJ43_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNZ71271.1"
                     /translation="MSILIKNIELNKKIQDILIEGNTITRIGSSIDFNADIEIEGTNK
                     AAVPGLFNGHTHAAMSLMRGYADDMPLMPWLQEKIWPLEKNITEEDVYWGTKLACLEM
                     IKTGTTLFIDMYHHAMASARATDEMGIRGMLTLAVLDFNDKEISEKFKKEIDQFHRAK
                     TAFSNRLEIALGPHAIYTVSKEMLRWMKEYARANNIRIQTHLSETEIEVKDSISMFGC
                     RPVNYLNSIGFLGPEISFAHSIHINDEEIKMLADNGCKVIHNPASNLKLASGTRFRYE
                     DLKKAGITVGIGTDGACSGNNLDMYEAMKLASLNAKAAWNDSTVWNADETFAAATKTA
                     EIITGFKTGTIDVGYLADIALIDLKKPEMTPNFNLISNLVYSANGSVVDTLICDGNIV
                     MKNRHVDGEEEIISKANEIAHNLVARKK"
     gene            complement(3786..4136)
                     /locus_tag="PKJ43_01550"
     CDS             complement(3786..4136)
                     /locus_tag="PKJ43_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNZ71272.1"
                     /translation="MNMIDAAAWAHEMDVRVTVCNKKGVIVYMNQASALGMHKYGGYE
                     LVGKSLFDCHNPASSAKIREMLEIPSVNVYVVEKQEERRLIRQFPWEENGEHKGIIEI
                     SFALPFDVEVKNRQ"
     gene            complement(4133..>4872)
                     /locus_tag="PKJ43_01555"
     CDS             complement(4133..>4872)
                     /locus_tag="PKJ43_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HNZ71273.1"
                     /translation="TLYFKYNETDSFKFFREWSLVLEETFSQKIDKSKWSFISPVAEK
                     GPGRNFSVKDQLQCYNDDKNIDTENGIFTLETHEEKKEGLYWDENFGFVLKDFNYTSG
                     LMHSNNFFKQEYGLFEMKLKASKVRGVISSVSLADDDENECIRLISLDFRKASGGIII
                     TEQGQRLISKVNMKHKPSGYLLLSVEWTPERIEWRVNDRFMGALSQNVPHEKLGLRIE
                     TEVIKPTSNLPHRLDIDWIRCYKRRKI"
BASE COUNT         1600 a         1041 c          884 g         1247 t
ORIGIN      
        1 cgccacaacc gcaaaagcct agatatgtcg aatatcagcc attttttaac caactcgaca
       61 aatcatttga gggaaataaa tatgcaaaaa acgaactcga caccgtggta aaaatccttt
      121 caaaccttcg tgaccaagta tacccaatta caacaacaat ttcattcaca gatgaatttg
      181 agctaagcaa aggagaattg cttatcgaca ttgaacttat taacgaaaaa cctcctttat
      241 acgacacctt catggttgtt aaagattttc ttatgagtga agagctgatg aatactctat
      301 tcccgttact aaacgatact actcttgaaa aatcaacccc tattgatacc ctgaaaaaaa
      361 tagaaacact ccctgaagaa aagccattta cccccacaaa agcgactaag tcaacaaaga
      421 ataaaccaaa aaaaatcacc agacgaggtt ctaaccccta caaaacaatc aatccaaaag
      481 ataaaagata ttcaagcaaa aagaagtact actaccccaa acaagcagaa aaacctaaaa
      541 aagccccaga accaattaag ccggcagaac cagagaaact gattacagaa gcccaagatt
      601 ctatcgtaaa aaaatcacgg aagttttaca cccgccaaga aatcctaagt gatacaacac
      661 gcgacctgct tgatgcagat actttgataa ccaacatttt cgctactact ggctggcaag
      721 tgggtgattc cattcccttt tttgctgaat atagcgacta cacccccgat tcatttatgc
      781 aacttgttac cgatatggtt tttgttgaac gctatgaccc ctcgagggaa gtcaaacaat
      841 acccgaaaga agaacttacc cgcgaagtag ctactaagaa aacagaaaac gaaaagtttg
      901 tttataatga taaggttgtt ttcagaaagt tttttaaaga cgaagaagat gttttcatca
      961 ccatggtaaa agtcaaaggc gatcgtttta aaatcgggaa taacgaattc gatgaagacg
     1021 aacgcccgga atacggcata caagtatctg attttctgat cggaaaatac gaaacaacca
     1081 atgaagtcgt atgctccttt ctgaatatgt taaaatgcga tgaaaaaggc tacagagaac
     1141 gaacaagagt atacaacacc aactctgaat tctccaagat atattgggac aataagatcg
     1201 gacggttcaa agtaattaaa gggtttgaaa aattccctgc tgtgaatatc acctggactg
     1261 gagctaacct tattggcaaa accggaggag gacggctgcc caccgaagct gaatgggagt
     1321 ttgccgcaaa aggaggtcgc tatgccatta aatatcacac caatagtgag cgaaccgatt
     1381 acgcatacga acatcgcttt gctggcagca ataccatggc caatgttggc tggtttgtcg
     1441 ataattcagg cggatactat catccggtgg gtgaacttat gcctaaccta ttggggattt
     1501 acgacatgtg tggaaatgtc tgggaatggt gtttcgacaa ctacgacaaa gaattttaca
     1561 aacgaaatgg cgatagcacc gaccccttct gcgtaaacaa taccagcctg aggtcaaacc
     1621 gcggtggaag ctggagcagc gatgcacaat attgcaggat tacgaaccgc aattttctcg
     1681 acgaatcaca atgcaaccaa ttccttggct tcagaatact caaagaatgg aacaggtaaa
     1741 gtcgtcaaca gtcatgtggg tcaatgatat caaatcttcg ggagcaattt ttatttgcat
     1801 gccccgcacc ccagcgctga tataaataaa atcgaataaa aggcacgact catcgataaa
     1861 gtttggataa gcttttttca tgccaatggc agtacaacca ccccgaatat agcctgttaa
     1921 tggcaacagt tctttcattg caatcatctc agcgttttta ttgcccgaag ctttggctgc
     1981 ttttttcaaa tcgacctctg agttgcccgg aataacaacc acaaaaatac cagacttatc
     2041 gccccgcaat accagtgttt tgaacacttg ttcgatatct tgtcccaact tcttcgcaac
     2101 agctattgca cttaaatctg attcatcaac ctcatattca atcaactcat aagagatatt
     2161 atgctgatcg agtatgcgta ctgcatttgt ttttttcatn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnna ttaccctgaa acaaatacaa aaatatatta tccggccaat
     2341 tatccccgac gaattgtttg tttaatcaga ataaacgata tttgagaagt taacaactcc
     2401 aacgagtctg ctcttgagtt cagagaatta atcacatata aaacaacaca atacgtaatt
     2461 ctaacccaaa ggacagacaa caataaacat tcgaaaaaat aaaaaaaaga cgatacgtca
     2521 tgagcatctt aataaaaaat atcgagctaa acaaaaagat tcaagatata ctaatcgagg
     2581 gcaacactat tacaagaatt ggctcttcaa ttgattttaa tgccgacatt gaaatagaag
     2641 gaacaaacaa ggctgcagta ccagggcttt tcaacggcca cacccatgca gccatgagcc
     2701 ttatgcgcgg ctatgccgac gatatgccac ttatgccttg gttacaggaa aaaatatggc
     2761 cactcgaaaa aaacataaca gaagaagacg tgtactgggg cacaaaacta gcctgcctgg
     2821 agatgatcaa aaccggcaca actcttttta tcgatatgta ccatcatgct atggcatctg
     2881 cccgcgccac cgatgaaatg gggatcaggg gaatgttaac ccttgctgtg cttgatttta
     2941 acgacaaaga gatttctgaa aaattcaaaa aagaaattga ccagttccac cgcgcaaaaa
     3001 cggccttttc aaacaggttg gaaattgccc ttggccctca tgccatttac accgtatcaa
     3061 aagaaatgtt gcgctggatg aaagagtatg cccgcgccaa taatataaga atccaaaccc
     3121 acctctctga aaccgaaatt gaagtgaaag attccatttc catgtttgga tgccgcccgg
     3181 taaattacct caactcgatt ggctttttgg gaccagaaat aagttttgcc cacagcatcc
     3241 acatcaacga tgaagaaatt aaaatgcttg ccgacaatgg gtgcaaggta attcataatc
     3301 cggcatcgaa tttaaaacta gcttctggca ctcgcttcag atatgaagac ctgaagaaag
     3361 ccggcatcac cgttggcatt gggaccgatg gggcttgttc aggtaacaac ctcgatatgt
     3421 atgaagcgat gaaattggct tcgcttaacg ccaaagcagc atggaacgat tccacagtct
     3481 ggaatgccga tgaaactttt gcagctgcaa ctaaaaccgc cgaaataata acaggcttca
     3541 aaacaggtac aattgacgtt ggataccttg cagacattgc ccttattgac ctgaaaaaac
     3601 cagaaatgac accaaatttt aatctgatat ccaacttggt atactcagcc aatggttcag
     3661 tggtcgatac acttatttgc gatggaaaca ttgtaatgaa gaaccgccat gtcgatggcg
     3721 aagaagaaat aataagtaag gcaaatgaaa ttgcacacaa cttagtagca cgaaagaaat
     3781 aatctttact gacggttttt cacttcaaca tcgaaaggta atgcaaaaga aatttcaatt
     3841 atgcctttgt gttcgccatt ttcctcccat ggaaattgcc gtataagtct tcgttcttct
     3901 tgcttttcaa caacataaac atttacagaa ggaatttcga gcatttctct aatttttgca
     3961 gatgaagccg gattgtgaca atcgaaaagt gatttgccca cgagttcata tccgccatac
     4021 ttatgcattc ccaatgcaga ggcttggttc atataaacta taacgccctt tttgttgcac
     4081 accgtaaccc ttacatccat ttcgtgggcc catgcagcag cgtcaatcat gttcatatct
     4141 tgcggcgttt gtagcaccta atccaatcaa tatcaagtcg atgcggcaaa ttagatgttg
     4201 gtttaataac ctcggtttca atacgcaagc ccaacttttc atgcggtaca ttttgcgaca
     4261 aagcccccat gaaacgatcg ttaacacgcc actctatacg ttcgggagtc cattcaactg
     4321 atagaagaag atagccagaa ggcttgtgtt tcatgttgac tttagagata aggcgttgtc
     4381 cttgttctgt aataataatt ccgcctgatg cttttctgaa atccagggaa ataagccgga
     4441 tacactcatt ttcatcatca tcggcaagag atacagagct aataactcca cgaactttag
     4501 atgcttttag cttcatttca aaaaggccat actcctgttt gaaaaaattg tttgaatgca
     4561 taagccccga agtgtagtta aaatctttca acacaaaacc gaagttttcg tcccaataaa
     4621 gtccctcttt cttttcttca tgagtctcca gggtaaaaat tccgttttct gtgtcgatat
     4681 ttttatcatc gttatagcat tgcaattggt ctttcaccga aaaattgcgg cctggtcctt
     4741 tttcagcaac aggcgaaata aaagaccatt tactcttatc gattttttgt gagaaagttt
     4801 cttcaagcac caatgaccat tcacggaaga atttaaaaga atcggtttcg ttgtatttga
     4861 aatataacgt ta
//
LOCUS       DAOPUW010000052         4823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_17678_length_4823_cov_2.639681, whole genome shotgun sequence.
ACCESSION   DAOPUW010000052 DAOPUW010000000
VERSION     DAOPUW010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17678_length_4823_cov_2.639681"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="PKJ43_01560"
     CDS             complement(<1..999)
                     /locus_tag="PKJ43_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNZ71274.1"
                     /translation="MKNILLIITSLLTSFLFAQNNPVALIPQPANAMMKEGFFILDNE
                     CAINYTPEAKKSALILAETISTMTGIKPIIGKNGVRNIFLEVNKVSNQQTGEEGYNLV
                     VEEKKITIKANTETGIFYGIQTLLQLIPTEEIWSKQQSGSTNIPAIEITDYPRFRWRG
                     AMLDVSRHFFTKEEIKTHIDQLSHYKFNTLHLHLTDDHGWRIEIESLPKLTDIGAWRV
                     ERQGQFGERENPKPGEPKHYGGFYTHNDIREIVEYAKEKYVTIVPEIDIPGHCMALLA
                     AYPNLSCTKDTSIQVNPGTHFSEWYSDGSFKMLVDNTLNPADENVYKFLDNVFTEIA"
     gene            complement(1507..2265)
                     /gene="tpiA"
                     /locus_tag="PKJ43_01565"
     CDS             complement(1507..2265)
                     /gene="tpiA"
                     /locus_tag="PKJ43_01565"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNZ71275.1"
                     /translation="MRKKVVAGNWKCNTTLQEGVELAKAVNQLVAEKGAKDVVVVLGT
                     PFTHLTSVISAIDSKRIGVAAQNCAAEAKGAFTGEVSASMVKSTGAEYVILGHSERRE
                     YYGETSEILNKKLALALENSLTPIYCCGEALAIRNAGTQNEYVINQLKETVFQLSAED
                     FKKIIIAYEPIWAIGTGVTATTEQAQEMHKALREAVAEKFGQEIANETSILYGGSCNA
                     KNANELFANPDVDGGLIGGASLKAEDFIQIINAY"
     gene            complement(2441..3715)
                     /locus_tag="PKJ43_01570"
     CDS             complement(2441..3715)
                     /locus_tag="PKJ43_01570"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HNZ71276.1"
                     /translation="MKVHALKVILISLLLSILTNTLIAGNIDKTIRYDVCFTGKADHW
                     DVIHSQTKIVSSATGNLNYEKHFADKGNMRLVVKETETGDTLFIKGFSPLFQEWLLTS
                     EANTKEREFYQALYFPHPSKDVLVSIDLRNIEGQWGQKYADTLFISDIRIKNEKPKKY
                     KIDTLLYSGHSQEKIDLVFLSEGYTREEMDKYVADAKRMARFLLDFQPFNEFQDKFNL
                     YAIQVPSAESGADEPEIGILKNTAFNATWNTFESERYLTSGDMLQISNAADEVSWDQI
                     VIIVNSARYGGGGFYNWLSIFSSDHQQSELVFIHEFGHAFAGLADEYYYNGDIEDTTY
                     PITIEPWEPNITTLVQFDKKWKGSISHKTPLPTPRDSMYINTIGLFEGGGYRTKGIYS
                     PSITCLMKTLSTKSFCPVCQEAIRQTILNQAK"
     gene            3941..>4823
                     /locus_tag="PKJ43_01575"
     CDS             3941..>4823
                     /locus_tag="PKJ43_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ71277.1"
                     /translation="MRVVLMLFFVVGCDIVSWAGNYVAKDITANVFTITPDRKQLHTL
                     GWPNDIINSVVINTGNGLILVDTQNSPANARLIKKGIMEIVNDTTFLYVINTHGHSCH
                     SGGNCVFSQNKIVAQINSHAEMKDYDDLFLGQTVEFLRQKIYYKSFILDTITSEGILS
                     DSIKQAIDLYKYYESDLLNNYKVRYPDLTFEDTLTLRAGSVSAKLIYMGKGHGDADVA
                     VYIPEEKVVCTGNLFHLGAYSQESMPSFYLNRVNEINKWIQSLNYLLDGKNPIDYVIS
                     THGRKPMNRRTLEFVRDY"
BASE COUNT         1276 a          904 c          972 g         1671 t
ORIGIN      
        1 tgcaatctcg gtaaaaacgt tgtccagaaa tttgtacaca ttttcgtcgg ctgggtttag
       61 tgtattgtcg accaacattt tgaatgagcc atctgaatac cattctgaaa aatgcgttcc
      121 tgggttgacc tgaattgagg tgtcttttgt gcagctcaaa ttggggtaag ccgccaatag
      181 agccatgcaa tgtccgggga tatctatttc gggaacaatg gtaacgtact tttctttagc
      241 atattcaact atttcacgaa tatcgttatg tgtataaaaa cctccgtagt gtttaggttc
      301 acctggcttg ggattttctc gttctccgaa ttgtccttgc cgttcaaccc tccatgcccc
      361 aatatctgta agtttgggta aggattcaat ttctatacgc cagccgtgat catcggtcaa
      421 gtgtaaatgc aacgtattaa atttatagtg tgataactgg tctatatggg ttttgatttc
      481 ttcttttgta aagaaatgcc ggctcacatc gagcattgca cctcgccatc ggaaacgagg
      541 ataatcggtg atttctatgg cgggaatatt agtgcttcct gattgttgct tgctccaaat
      601 ttcttctgta gggataagtt gcagcaatgt ttgtatgcca tagaaaattc cggtttcagt
      661 gttagctttt attgtgatct ttttttcctc tacgactaga ttatatcctt cttctcctgt
      721 ttgctgattg ctaaccttat tgacttcgag aaaaatattt cttaccccat ttttcccgat
      781 gatgggcttt attccggtca ttgtcgaaat cgtttcagca agaattaatg ctgatttttt
      841 tgcctcagga gtatagttta tagcacattc attatcaaga ataaaaaagc cttctttcat
      901 cattgcatta gctggttggg ggattaatgc aacaggattg ttttgggcaa aaaggaatga
      961 tgtgagcagc gatgtgatta tcagtaatat attcttcatt attaactggt ttttccattt
     1021 tgttctcctg ttggagaaaa ccagccctac atttggggca acctttctcc gttgcgggaa
     1081 aattagcccg agaccgcgag caagctttct cctgcaggag atgatgtgcc cgttaccggg
     1141 ttggggttct ctccatgcag ggatgttttt tttctttcaa tttagatcat tttcaatagc
     1201 ttttcaaata aatcagagtt cgtctgctta atttttctaa aggcatatgt ccgaattgga
     1261 tcattacaag aaggtcttcc gtgtttatct gctcatggat acgtaaaggt ttttcatgat
     1321 tgaaaaatac ataatctaca ttaaacgttc ttgaacgtta taatgttttt ttaagttcta
     1381 ttttattttt tgtcaggtct gttttagttc acgaaaagta tgggtacaaa aaaacagagg
     1441 ctacacttta aaaagaatag cctccggtaa ggtgaaattt aggatttgta tactatagat
     1501 aattatctag tatgcattga taatttgaat gaaatcttct gccttaagtg atgcaccacc
     1561 aattagccca ccatcaacat cgggattggc aaaaagttca ttagcatttt ttgcattgca
     1621 gcttcctccg taaagaattg atgtttcgtt ggcaatttcc tgaccaaatt tttcagcaac
     1681 tgcttcgcga agtgctttgt gcatttcttg tgcctgttcg gttgtggctg ttacgccagt
     1741 accgatagcc cagatgggtt cgtaagcaat aatgattttt ttgaagtcct cagcagaaag
     1801 ctggaatacg gtttctttca actgattaat tacatattcg ttttgagttc ctgcgttacg
     1861 aatggcaagt gcttcgccac agcaataaat tggggtaaga ctattttcga gggcaagtgc
     1921 taatttttta ttcaatattt cacttgtttc accgtaatat tcacggcgtt cgctatggcc
     1981 taagattaca tattcagctc cggtagattt taccatcgat gctgatactt cgccggtgaa
     2041 agctcccttg gcttctgcag cacagttttg ggcagcaaca cctatgcgtt tgctatctat
     2101 agcactgatt acgctggtaa gatgtgtgaa cggagttcct aaaacaacca ctacatcttt
     2161 tgcgcctttt tcagcaacta attggtttac agcttttgca agctcaacgc cttcctggag
     2221 ggtggtattg catttccagt taccggcaac tacttttttc ctcatttttc agaatatttt
     2281 ttgaaaagtt tatttttctt ttgaagtccc gcaaaattat taaaaaagca taaatctttt
     2341 aatgaagggc ttgttttttc gcatcgttaa caatgatata ataccgtatc ggtttttcac
     2401 tatcttggta acgtgttgtt ttgtacttgt ttgctgattg ctattttgcc tggtttaaaa
     2461 ttgtttgcct gattgcttcc tggcatacgg ggcaaaaact ttttgtagat agtgttttca
     2521 ttaagcaagt tatagaagga ctgtatattc ctttagtcct gtatccgccg ccttcaaaca
     2581 gcccgatggt gtttatatac atcgaatcgc gtggggtagg caggggagtt ttgtgagaaa
     2641 tacttccctt ccactttttg tcaaattgca ccagtgtggt aatgttgggc tcccatggtt
     2701 caatagtaat ggggtaggtg gtatcctcaa tatcgccgtt gtagtaatat tcatcggcca
     2761 aaccggcaaa tgcatggcca aattcatgga taaaaactaa ttcagattgt tggtggtctg
     2821 acgagaaaat gcttaaccaa ttgtaaaagc cacctccgcc atatcgggca gaattcacaa
     2881 taatgactat ttgatcccac gatacctcat cggcagcatt tgaaatttgc agcatatcac
     2941 cggacgtgag gtatctttcc gattcgaatg tattccatgt ggcattgaac gcagtatttt
     3001 ttagtatacc aatctcaggt tcgtcggctc cgctttcagc cgaaggtact tgtatggcat
     3061 aaaggttgaa tttatcttga aattcattaa atggttggaa atcaagaagg aatcgggcca
     3121 tgcgtttagc atcagcaaca tatttatcca tttcttcgcg cgtataacct tctgacaaaa
     3181 atacaagatc gatcttttcc tgtgaatgac cactgtatag caaggtgtcg attttgtatt
     3241 tctttggttt ttcatttttt attctaatgt cagaaatgaa tagtgtgtct gcatatttct
     3301 gcccccattg tccttcaatg tttcttaagt cgatgcttac cagtacatct ttcgatgggt
     3361 gtggaaagta aagggcttgg tagaattctc gttctttggt attcgcttcg ctggttagca
     3421 accattcctg aaaaagcgga ctgaagcctt ttataaaaag cgtgtcgcct gtttctgttt
     3481 cttttactac caatcgcatg ttgcccttat ctgcaaaatg tttttcgtaa tttaagtttc
     3541 ctgtagcaga gcttacaatt ttggtttgag aatgaataac gtcccaatga tctgctttgc
     3601 cggtaaaaca tacatcgtaa cgtattgttt tatcaatgtt ccctgctatt agcgtattgg
     3661 ttaaaatgct taacaacaat gatataagaa tgactttcaa ggcgtgcact ttcacttttt
     3721 tcttttcttg tattttttta atcttgatac ttttacaagt ttacgaagaa tatttcagcc
     3781 gaactttttt ttgagttatt ttccttcttt tgggtgacta atttcctaat agctagtatt
     3841 attcgtcgta aacgatcatg attaacggaa agtagtccga ttttattttc ttatttctta
     3901 aatttacccc gttaatgaat aacaagaaaa ggatagagat atgagggtag tgttgatgtt
     3961 atttttcgtt gttggttgtg atatagtgag ttgggccggg aattatgttg caaaagatat
     4021 aactgcaaat gtatttacca ttacccccga ccgaaaacag cttcatacct taggctggcc
     4081 aaacgatata attaattccg tcgtaataaa taccggcaac gggttaattt tggtcgatac
     4141 gcaaaactct ccggccaatg cacggctgat caaaaaaggg attatggaga ttgtgaacga
     4201 tacaacattc ttgtatgtta ttaatactca tggtcactca tgtcattctg gcgggaattg
     4261 tgtttttagt cagaataaaa ttgttgccca aatcaacagc catgccgaga tgaaagatta
     4321 cgacgatttg tttcttggcc aaaccgttga gtttttgaga cagaaaattt attataaatc
     4381 gtttatcctc gacaccataa cttccgaagg cattctttca gattcgataa agcaagccat
     4441 cgatttatac aagtattatg agtctgattt attgaacaat tataaagttc ggtatcccga
     4501 cttaactttt gaagataccc tcacacttcg tgccggttct gtttctgcaa aactcatcta
     4561 catgggcaag gggcatggcg acgccgatgt tgccgtttat attcctgaag aaaaggtggt
     4621 ttgtacgggc aatttgtttc acctgggggc ttatagtcag gaaagtatgc ctagttttta
     4681 tctgaatagg gtcaatgaaa taaataaatg gattcagtcg ctgaactatt tgcttgacgg
     4741 gaaaaaccca attgattatg ttatcagtac ccacgggcgt aagccgatga acaggcgcac
     4801 gctggaattt gttcgcgatt att
//
LOCUS       DAOPUW010000053         4819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_17692_length_4819_cov_3.476910, whole genome shotgun sequence.
ACCESSION   DAOPUW010000053 DAOPUW010000000
VERSION     DAOPUW010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17692_length_4819_cov_3.476910"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="PKJ43_01580"
     CDS             complement(<1..1023)
                     /locus_tag="PKJ43_01580"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNZ71278.1"
                     /translation="MQLLLLTIMTEKDYTGKKVAFCTLGCKLNFSETSSMARKFEALG
                     FERVDYKEVADVYVLNSCSVTAESDKKSRNLIRSAIKKNPQAVMMVTGCYAQLRSSDI
                     ADIQGVDYILGSGEKMNITSYVNDLAKCEQPVLKVTDYRKINNYFSAYSYGDRTRTFL
                     KVQDGCNYFCTYCTIPMARGKSRNDSIENIVAEARIIAEKQIKEIILTGVNIGDFGRS
                     SNETFLQLVEALDEVEGIERIRISSVEPNLLTDDIIDFVASSKRFAPHFHIPLQSGSN
                     DVLALMKRKYKREVFASRVQKIKHLIPDAFIGVDVIAGTNGETESFFNDSYNFIRDLD
                     VSQLHVF"
     gene            complement(1090..1617)
                     /locus_tag="PKJ43_01585"
     CDS             complement(1090..1617)
                     /locus_tag="PKJ43_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNZ71279.1"
                     /translation="MRKIGLLSDTHCFLDERIFSFFESCDEIWHAGDFGDVATADRLA
                     AFKPLKGVYGNIDGQEIRAMFPKHLKFKCEGVKVWMTHIGGYPGRYDSSVKPGIYDDP
                     PNLFISGHSHILKVVNDKKLKLLHINPGAAGNYGFHHVKTLVRFTIDGADIKDLELME
                     MERNHKIGFSASDKG"
     gene            1751..2242
                     /locus_tag="PKJ43_01590"
     CDS             1751..2242
                     /locus_tag="PKJ43_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNZ71280.1"
                     /translation="MKRLILVRHAKAEQGGYSNDFARELTDRGKNDARTISSDMKRRE
                     IIPDYIITSPAKRALTTARIFAEETGFDKSKIVEQKGLYFDYTTNDFIEMLNEIDDKY
                     ETVAVFGHNPFIYFMAENLCQHFGGDMPTCSTIVIDFDISSWKKVEARQGKLIIRLYP
                     SQY"
     gene            2271..2984
                     /locus_tag="PKJ43_01595"
     CDS             2271..2984
                     /locus_tag="PKJ43_01595"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HNZ71281.1"
                     /translation="MNIFYTPDISGSHYTLDENESKHCIRVLRKTKGDEIILVDGKGG
                     YYQTIITDDNPKRCSIEVKNTIPEFGKRNYSLHLAIAPTKNIDRFEWFIEKATEIGID
                     EITPLLCEHSERKQVNNERLEKILISAMKQSLKAQLPKLNNLTNFETLISGTKCDGKF
                     IAHCEEEAKQHLFTAAKPFNNSVVLIGPEGDFSTTEIQKAIESGFKAVTLGESRLRTE
                     TAGVVACEIIRLANEISML"
     gene            3071..3757
                     /locus_tag="PKJ43_01600"
     CDS             3071..3757
                     /locus_tag="PKJ43_01600"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNZ71282.1"
                     /translation="MTHNIRESIYFILLFFIAFAVSGQQKTTVKIGLLKYNGGGDWYA
                     NPTSLPNLIDFCNKNLNTNIHPEPGTVEPGSMELFSYPLIHMTGHGNVIFSDYEVEML
                     RTYLMGGGFLHIDDNYGLDEYIRREMKKILPDQEFVELPVTHPIYHQHYNFDKGLPKI
                     HEHDNKPAQGFGLFHDNRLVVFYTYECDLGDGWESYAVHKDPEHIRLLALQMGANIVS
                     YVFDRKESVP"
     gene            3769..4347
                     /locus_tag="PKJ43_01605"
     CDS             3769..4347
                     /locus_tag="PKJ43_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNZ71283.1"
                     /translation="MIKVVAFNGSPRKEGNTSILIRSVFEELNKEGIETEVIQLGNKN
                     VHGCTGCGKCREVQNKKCQIKNDLLNTCIEKMIEADGIILGSPVYFADVTSEMKALID
                     VAGYVTRGNGHLLARKVGAGVVAVRRAGEIHALNSMNNFFMINQMIVPGSSYWNMAQG
                     REPGDVLNDTEGMQTMKRLGENMAWLLNKLKA"
     gene            4487..>4819
                     /locus_tag="PKJ43_01610"
     CDS             4487..>4819
                     /locus_tag="PKJ43_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HNZ71284.1"
                     /translation="MEKLNKKFYEQELERLYIELVKLQTTVKKKGLRVVVLFEGRDAA
                     GKGGAIKRIAEALNPRICRVVALSVPTEKEKTQWYFQRYVSHLPAAGEIVLFDRSWYN
                     RAGVEKVMG"
BASE COUNT         1609 a          996 c          931 g         1283 t
ORIGIN      
        1 aaatacatgg agttgactga cgtccagatc cctgatgaaa ttatacgaat cgttgaaaaa
       61 gctttcagtt tcgccatttg ttccggcaat aacatctacc ccaataaatg catcagggat
      121 caagtgtttt attttttgaa ctcttgaagc aaatacttcg cgcttatatt ttcgtttcat
      181 caatgccaaa acatcgttcg aacccgattg taatgggata tggaaatgtg gggcaaaacg
      241 tttcgatgaa gctacaaaat caataatatc atcggtaagc aagttgggct caactgatga
      301 aatcctgata cgttctatcc cttctacttc atcgagagcc tcgaccagtt gcaaaaaagt
      361 ttcgttggag cttcgcccaa aatcaccaat attaaccccg gtaagaatta tctctttgat
      421 ctgtttttct gcaattatcc gggcttcagc aactatattt tcaattgaat cgttcctgct
      481 tttgccacgc gccataggta tagtgcaata cgtgcaaaaa tagttgcatc catcctgaac
      541 ttttaaaaag gtccgggtac gatcgccata cgaatatgct gaaaaataat tgtttatctt
      601 acggtaatcg gtaactttta atacaggttg ttcacacttg gcaagatcgt tcacataact
      661 cgtaatattc attttttcgc ctgaaccaag aatataatcc accccttgga tatctgcaat
      721 atcactactg cgaagttgtg cataacaacc tgtgaccatc attactgcct gtggattttt
      781 tttgatagca gaacgaataa gatttctgct tttcttgtca ctttcagcag taaccgaaca
      841 ggaattaagc acataaacat cggcaacttc tttatagtcg actcgttcaa agccaagcgc
      901 ttcgaatttc cgtgccatcg acgaagtctc tgagaaatta agtttgcagc ctaaggtgca
      961 aaaagccact ttctttcctg tatagtcctt ttcggtcata atggttaata ataaaagttg
     1021 caaaaataca actttgcagg ataaccccac ctacaatcat agctggaacg aaagattttt
     1081 aagacaaatt tatcctttat cagaagctga aaaaccgatt ttatggttcc gttccatttc
     1141 catcagttca agatctttaa tatcagcacc atcaatagta aaacgcacga gggttttcac
     1201 atgatgaaat ccataatttc cggcagcccc gggattaata tgtaacagct tcaatttctt
     1261 gtcattaaca actttaagaa tatgtgaatg acccgaaatg aacaaatttg gaggatcatc
     1321 gtaaatgccc ggtttaacgc ttgaatcgta acgcccgggg taacctccaa tatgggtcat
     1381 ccaaacctta accccctcac acttaaattt taaatgcttg ggaaacatcg ccctgatttc
     1441 ctgcccatcg atatttccat acaccccctt aagtggttta aaagcagcta aacgatcagc
     1501 cgtagcaaca tcgccgaagt cgcctgcatg ccatatttca tcacaactct cgaaaaatga
     1561 aaaaattctt tcatcgagaa aacagtgagt atctgagagt aaacctattt tcctcattat
     1621 aaatttttaa cgtcgaaaag taacaaaaaa tagctagttg aaccgtattt ttgtattttg
     1681 gaacggttgc aaaaaggtaa aatcaaaaat ctctttgtcc gacattagaa caacaagaaa
     1741 tacattacaa atgaaacgat tgatattggt tagacatgct aaagccgaac aaggcggtta
     1801 tagcaacgac ttcgcgaggg aactcaccga ccgggggaaa aatgatgccc gtaccatttc
     1861 ttcagacatg aaacgaaggg aaattattcc tgattatatt attaccagtc cagcaaaaag
     1921 agccctaacc acagcccgca ttttcgccga agaaactggt ttcgataaaa gcaaaatcgt
     1981 agagcaaaaa ggactttatt tcgactatac aactaacgat tttattgaaa tgctcaacga
     2041 aattgacgac aaatacgaaa cagttgctgt ttttgggcac aaccctttta tatattttat
     2101 ggctgaaaac ctttgccagc attttggcgg cgacatgccc acctgctcaa caatagtaat
     2161 tgattttgat atttcttcat ggaaaaaagt tgaagcacga caaggaaagc tcattattcg
     2221 cctgtatccc agtcaatatt gaaaaagcta attttaaatt cgctacaatc atgaacatat
     2281 tttacacacc cgatataagc ggttcgcact atacgctcga tgaaaacgag tcgaagcatt
     2341 gtatccgcgt attacgaaaa accaaaggag acgaaatcat tcttgttgat ggcaaagggg
     2401 gatattatca aaccattata accgacgaca atcccaaaag atgcagcatt gaagtaaaaa
     2461 ataccattcc cgagtttgga aaaagaaact actccctgca tttggctatt gccccaacca
     2521 aaaacatcga ccggtttgaa tggtttattg aaaaggcaac cgaaattgga atcgatgaaa
     2581 tcacgcccct tttatgcgag cattctgagc gaaaacaagt taataatgaa agacttgaaa
     2641 aaatattaat ttcagcaatg aagcaatcgc taaaagcaca actgccaaag ttgaataatt
     2701 taactaattt tgaaacgtta atttcgggaa cgaaatgcga tggaaagttc attgcacatt
     2761 gcgaagaaga agctaagcag caccttttta ctgccgcaaa accattcaat aattctgtgg
     2821 ttttaattgg gcctgaagga gatttctcga ctaccgagat acagaaagct atcgaatcag
     2881 gcttcaaggc agttacatta ggagaaagcc gccttcgcac cgaaactgca ggagttgtgg
     2941 cctgcgaaat aatacgtttg gcaaatgaaa taagcatgtt gtgaaaccat tattcgcgca
     3001 cagatgatta tcttcattgc atagaagaag ttgacaaatt tgtcacaaac caaaaaaatg
     3061 aattgaacag atgacacata acataaggga aagtatctat tttatactac tgtttttcat
     3121 tgcatttgcc gtatctgggc agcaaaaaac cacagtgaaa atcgggctct taaaatacaa
     3181 cggagggggc gattggtacg ccaaccctac ttcgttaccc aatcttattg acttttgcaa
     3241 caagaacctt aacaccaata tccacccgga accaggtaca gtagaaccgg gaagtatgga
     3301 acttttcagc taccctctca tccacatgac ggggcatgga aacgtgatat tttccgacta
     3361 tgaagtagaa atgctgcgta cctatttaat gggaggcggc tttctgcaca tcgacgataa
     3421 ttatgggctc gacgaatata tacgccgtga gatgaagaaa attctccccg atcaggagtt
     3481 tgtagaattg cctgtgacac atccaattta tcaccagcac tacaatttcg acaaaggctt
     3541 gccaaaaatt cacgaacacg ataataaacc ggctcaaggc ttcggacttt tccacgataa
     3601 ccgtttagtt gtattttaca cctatgaatg cgatttaggt gacggctggg aatcgtacgc
     3661 agttcacaaa gaccctgagc acattcggct tctggcactg caaatgggag ccaacattgt
     3721 atcatacgtt ttcgacagaa aagaaagcgt accttaatca agatcaacat gataaaagtt
     3781 gtagcattta atggtagtcc ccgaaaagaa ggaaatacca gtattcttat ccgctcggtt
     3841 tttgaagaat taaacaaaga aggcatcgaa acagaagtaa tccaacttgg aaataaaaac
     3901 gtacacggct gcaccggatg cggaaaatgc cgcgaagtac aaaacaaaaa atgtcagatt
     3961 aaaaatgact tactgaatac ctgcatagag aaaatgattg aagccgacgg gataattcta
     4021 ggatcacccg tttattttgc agacgttaca tccgaaatga aagccttgat cgatgttgca
     4081 ggttatgtta cccggggaaa cggacatttg cttgcccgaa aagtaggtgc tggagttgtt
     4141 gcagttaggc gtgcaggaga aatacatgcc ctcaacagca tgaataattt tttcatgatt
     4201 aaccagatga ttgtgcctgg atcttcatat tggaatatgg cccaaggccg tgagcccggc
     4261 gatgtcctta atgataccga aggcatgcaa acaatgaaac ggctgggcga gaatatggca
     4321 tggcttttga ataaactgaa agcataacca ttttttgttg tcaaaaacaa tgagaaataa
     4381 aattcgaaac gcataagaag cttaagaatt caacaaaaaa tgagaactaa atgcaagcta
     4441 taaaacaaat atccgtaact tgaatgctga accaaatttg tcaccgatgg aaaaactgaa
     4501 caagaaattt tacgaacaag aacttgaacg gctttatatc gaactagtta agctacaaac
     4561 caccgttaag aaaaaaggat tacgtgtagt ggtacttttt gaaggcagag atgctgccgg
     4621 taaaggcggt gcaataaaac ggatagccga agcccttaac ccccgcattt gccgtgtagt
     4681 cgcgttaagt gtacccaccg aaaaagaaaa aacgcagtgg tactttcaac gttacgtctc
     4741 acacttgcct gccgccggtg aaattgtgct cttcgaccgt agctggtata accgggcagg
     4801 agttgagaaa gtaatgggt
//
LOCUS       DAOPUW010000054         4811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_17741_length_4811_cov_2.579268, whole genome shotgun sequence.
ACCESSION   DAOPUW010000054 DAOPUW010000000
VERSION     DAOPUW010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17741_length_4811_cov_2.579268"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1998
                     /gene="ccsA"
                     /locus_tag="PKJ43_01615"
     CDS             <1..1998
                     /gene="ccsA"
                     /locus_tag="PKJ43_01615"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNZ71285.1"
                     /translation="YPGSQSPSSYSSYVKVSDSEKNQHFDYHIYMNHILHYRGWRFFQ
                     TSYDSDEKGTILTATRDTIGTPLTYAGYTLAILLMFFSLFMPGSYFRRLLSELKKSTV
                     IILLIITPLIAISKPIDQSKVVGIKQASDFGELIIQDSKGRMKPMNTLNNEFMRKIYG
                     KETFEGLSSNQVVLSMAVYPDYWKNVPLIKVKNEEIKNTLSIGSSHIPFSALLDKNGE
                     YRLAEIANRAFMSAPATRTATDKALIKLDENVNIFSHFLRGDAYAIFPSEKAENNKWH
                     TPTDAALFTSNPEDSLFVANVFKLYLLELVEGNSNIAGNKAEKYLDAIKMYQQTIGKK
                     VLPSPTKIKAELFYNKANIFNLSTYALLAAGLSLLFFFFYTTIKGAQLSNNILLIYKA
                     IAVILLTFVASGIALRWYIGGYIPMSNSYEVTIFIAWVSLLTGLLLCGKQPVVLALSM
                     ILSFAFLLVSAMNNGNPEIGTLVPVLKSYWLSIHVAVITSSYALFALAMMLALVNLSM
                     FAFIPSKKFEGYLKTSKQIVLLEQILIIPGLYLLTIGTILGAVWANESWGRYWGWDPK
                     ESWALISIIVYALVSHLRLIPSFKDEIWFDLSVFWAFASVVMTFWGVNYLLSGLHSYG
                     QNGQGEIPIGLTITLIVLIVFSAFAIVRRIHLSDSSKNTTA"
     gene            2237..3127
                     /locus_tag="PKJ43_01620"
     CDS             2237..3127
                     /locus_tag="PKJ43_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNZ71286.1"
                     /translation="MKSTNTLYIQIMKTTNFKLKLISITTILLFAFSVNAQNPNFYIF
                     ICFGQSNMEGQGPIEAQDLVGDPRFKVFQSLDCPNLNRTKASWYTATPPLCQCYSKLS
                     PADYFGRTLIENLPDSITVGVINVAVGGCDIRLFDKDIYMNYDSTYTEDWFTSKVSDY
                     GMNPYKHMIDLAKLAQKDGVIKGILLHQGETNTGQIEWPTYVKKIYQDMLADLSLNAS
                     QVPLLAGEVVSAEGSCCSSMNEIINQLPGTIPTAHVISSKGCQSQDNAHFNAEGYRIL
                     GGRYGKKMLTLLGFHSGNAE"
     gene            3244..4011
                     /locus_tag="PKJ43_01625"
     CDS             3244..4011
                     /locus_tag="PKJ43_01625"
                     /inference="COORDINATES: protein motif:HMM:NF025626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNZ71287.1"
                     /translation="MEKIIRLNKDNIDSEHICCAFSDKKCQESYALKKMWLKQQFDQG
                     YTFRRIDARAKVFIEYCPAEYSWNPIAAPNYLAINCFWVSGQYKGQGYGKQLLQHAID
                     DAKAQHKSGLVTIVGTHKFHFMSDTKWLLKQGFVSCDATSTGFSLLTLKFDNNAPSPY
                     FNAKAKQGTCDEKKGIVVYYSNRCAFTDYHVNNSLKETAKKRKLPLTIIKLETNEQAQ
                     AAPCPATIFGLFLNGNFVTTDLSVCMDSRFDKIVKII"
     gene            4333..4755
                     /locus_tag="PKJ43_01630"
     CDS             4333..4755
                     /locus_tag="PKJ43_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNZ71288.1"
                     /translation="MKYSLEQVINLLLSKSEQLEVDLKRESEIEELSIKQLQCIELIV
                     ELENPTLTELSERLKITKPSTTALIDKLESWGYVVKVKSDLDKRSAHVHLTEKGGSAG
                     KLHENVHHTIAKQLCGNLTDSEKEILIVLLNKAIQSFD"
BASE COUNT         1554 a          982 c          902 g         1373 t
ORIGIN      
        1 taccctggtt cccagtcgcc atcatcctat tcaagctacg taaaagtatc tgactccgag
       61 aagaaccagc acttcgatta tcacatttac atgaaccaca tattgcatta tcggggatgg
      121 cgtttttttc aaacttcgta cgatagcgat gagaagggca ctatactaac tgccacaaga
      181 gacaccatag gaactccctt aacctatgct ggctatacct tggctatttt actgatgttt
      241 ttttcacttt ttatgcccgg atcgtatttt cgaagactat tgtctgaact aaaaaaatca
      301 accgtaatca tcctactgat tatcacacca ttgattgcta ttagtaaacc aatcgaccaa
      361 tcaaaagttg tagggataaa acaagcttcc gattttgggg agctaatcat tcaagactca
      421 aaaggacgca tgaagccaat gaataccctc aataacgaat tcatgcggaa aatatatggc
      481 aaagaaactt ttgaagggct cagctccaat caggtagtac tttcaatggc agtttacccc
      541 gattattgga aaaatgttcc cttaatcaag gtcaagaacg aagaaatcaa aaatacttta
      601 agcatcggat catctcatat accattcagt gcattgctcg acaaaaatgg agaataccgg
      661 ctggcggaga ttgcaaaccg ggcattcatg agtgctccgg caacccgcac ggctacagat
      721 aaagccttga taaaactcga tgaaaatgtg aatatcttca gccacttttt acgtggcgat
      781 gcctatgcca ttttcccatc agaaaaagca gaaaacaaca aatggcatac ccctaccgat
      841 gcagcacttt ttacctccaa ccccgaagat tctctttttg tcgccaatgt tttcaagctt
      901 tatttgcttg agttggtcga agggaatagc aacattgctg gcaataaagc agaaaagtat
      961 cttgacgcaa taaaaatgta tcagcaaact ataggaaaaa aagtactccc ctcgccaaca
     1021 aaaatcaagg ctgaactttt ttacaacaag gcaaacatat tcaacttatc aacctatgcc
     1081 ctattggccg cagggctctc gttgctcttt ttcttttttt acacaacaat aaagggtgcc
     1141 caactcagca acaacatatt attgatttat aaagctatag ctgtaattct tctgacattt
     1201 gttgcctcag gcattgcatt gcgctggtac attggcggct atattcctat gagtaattct
     1261 tacgaagtaa ccattttcat tgcctgggtg agtttgctca cgggactgct tttatgtgga
     1321 aaacaaccgg ttgtactggc tttgtcaatg attctatcgt ttgctttttt gctggtatca
     1381 gccatgaata acgggaaccc cgaaattgga acattggttc cagtacttaa atcatactgg
     1441 ttaagtattc atgtggctgt aatcacctca tcatacgcac tttttgccct tgccatgatg
     1501 cttgctctgg taaatttgtc gatgtttgcc ttcatcccat caaagaaatt tgaaggatac
     1561 ctgaaaacaa gcaagcaaat agtgcttctt gaacaaatat tgataatccc ggggttgtac
     1621 ctgcttacca tcggtaccat tcttggggct gtatgggcca acgaatcgtg ggggcgttac
     1681 tggggctggg accctaaaga atcatgggca ctgataagca ttattgtgta tgctttggta
     1741 tcgcacctga ggctaattcc atcttttaag gatgaaattt ggtttgattt gtcagtattc
     1801 tgggcttttg cctcggttgt aatgactttc tggggagtga attatcttct ttccgggctt
     1861 cattcgtacg gacaaaacgg acaaggagaa atacccatag ggcttaccat cacgctaatt
     1921 gtgctgattg tattctcagc cttcgccatt gtacgaagga tacatctaag cgattcatcg
     1981 aaaaacacaa cagcttaacc tactgctgta atgaacatta cagatttact taacctgaga
     2041 ataaagatgg ttgataataa aaatacatct atagttttca ttctcaaggg ttattgttac
     2101 tttcgatgat tatttaccaa tcaacaaaca ctgtattaag acacaacgaa tcaatctaat
     2161 acattagtaa tatatttcat ttcagcgtca actaaaagta tttttgaaac gagttaaacg
     2221 attggtaaaa acgcaaatga aaagtacaaa tacactctat attcagatta tgaaaacaac
     2281 gaacttcaaa ttgaaactta tttcaataac aacaatactg ttgttcgcgt ttagcgtaaa
     2341 tgcccaaaac ccaaatttct atatttttat ctgttttggt cagtcgaaca tggaaggtca
     2401 gggacccatt gaagctcaag accttgttgg ggaccctcgt tttaaagttt ttcaatcgct
     2461 cgactgtcct aaccttaacc gcaccaaagc ttcatggtat actgccactc ctccactgtg
     2521 ccaatgttac tcaaaacttt cacccgccga ttatttcggt cgaacattga ttgaaaacct
     2581 gccggatagc attactgttg gcgtaatcaa cgttgccgtt ggaggttgcg acataaggct
     2641 tttcgataaa gatatttaca tgaattatga ctcaacgtat accgaagact ggtttacttc
     2701 gaaagtttca gactatggga tgaacccata caagcacatg atcgacttag caaaacttgc
     2761 tcaaaaagat ggagttatta aaggaatttt gctgcatcag ggggaaacaa ataccgggca
     2821 gatagaatgg cccacgtatg taaaaaagat ttatcaggat atgcttgccg acctttcact
     2881 caatgccagc caggtgcctt tactcgccgg cgaggttgta tcagccgaag ggagctgttg
     2941 ctcttccatg aatgaaataa tcaaccaact ccctggaaca attcctacgg cacacgtaat
     3001 atcatctaaa gggtgccagt cacaagataa tgctcatttc aacgctgaag gatatcgtat
     3061 tcttggtgga cgatatggta aaaagatgct tacgctatta ggctttcact caggcaacgc
     3121 tgagtaaaat aaataatgtt atacaagcac tcatcaacaa aagaacaatt ttatattcag
     3181 ctactgacat aagattgtca cacacatatt ttacttttag taaaaatgac tgaaaggaat
     3241 actatggaaa aaattattag actcaacaaa gataatattg atagtgaaca catttgttgt
     3301 gccttttccg acaagaaatg ccaggaaagt tatgcattga aaaaaatgtg gttaaaacaa
     3361 caattcgacc aagggtatac ctttagacgg attgatgcaa gagcaaaagt gtttattgaa
     3421 tactgcccgg cagagtattc atggaatcca attgccgccc caaactactt agcaatcaat
     3481 tgtttttggg tctctggcca atataaagga caaggctatg gaaaacagtt acttcagcat
     3541 gccatagacg atgctaaagc tcagcataaa tctgggctgg taaccattgt tgggactcat
     3601 aaatttcatt ttatgagcga cacaaagtgg ctactcaagc agggatttgt aagttgcgac
     3661 gccacatcaa cggggtttag tcttttaaca cttaaattcg acaacaatgc accttctcct
     3721 tatttcaatg ctaaggccaa acaaggcacg tgcgatgaaa aaaaagggat agttgtgtat
     3781 tattcaaacc gttgtgcttt taccgattac catgtgaata actcgttaaa agaaacggca
     3841 aaaaaaagaa aactcccttt gaccataatc aagctggaga ccaatgaaca agcccaagcc
     3901 gccccatgcc ctgcaacaat ttttggcctg tttttaaacg gaaactttgt taccaccgac
     3961 ctcagtgttt gtatggacag ccgtttcgac aagattgtga aaatcatata aacgccaagt
     4021 tatcataaac aattgaagaa caacttactt cagccctaaa atcaataaga gtgaaagatt
     4081 tgaattcaat acttctcatt taatgaagtc gcttgaattg tattcttctt caaatcttga
     4141 aaaatggtct caatcgtata aaaaggaata tgagtagaat tcaccatatg atattcatta
     4201 acaacaaatt aaatcaaaca atttttcctc agagtacatt cagatattaa aattcagtaa
     4261 aaaacaaaaa atatttcatg taattagttc gatataacga actaattcta tctttgcaga
     4321 aaatttacag tcatgaagta ttcattggaa caagtaatca acctgcttct gtcgaaaagc
     4381 gagcaattgg aagtcgacct gaaacgcgag tcggagattg aagagctgtc gattaaacag
     4441 ttacagtgta ttgaactgat tgtagaattg gaaaatccca cgctcacaga attgtctgaa
     4501 aggcttaaaa ttaccaagcc atcgaccacg gcgttgatcg acaagctcga aagctgggga
     4561 tatgttgtga aagtaaagtc tgatttggac aagcgatcgg cccacgtgca tctgaccgaa
     4621 aaagggggaa gtgccggcaa actccatgaa aatgtgcacc atactatcgc caaacaactt
     4681 tgcggaaacc tgaccgactc tgaaaaggag atcctcattg tgcttctaaa caaagccatt
     4741 cagtcgttcg actgatattt tttttggaaa gttatttact tagttttatc taactaatta
     4801 tgaaatcaat t
//
LOCUS       DAOPUW010000055         4792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_17852_length_4792_cov_3.228415, whole genome shotgun sequence.
ACCESSION   DAOPUW010000055 DAOPUW010000000
VERSION     DAOPUW010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17852_length_4792_cov_3.228415"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="PKJ43_01635"
     CDS             complement(<1..1276)
                     /locus_tag="PKJ43_01635"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNZ71289.1"
                     /translation="MRQITLILIMLLLVFTGCNSSKTKKSPLSKTGGQPSRTTGWQAN
                     DMEVFGFTDPAYIEQQTGPGLVLVQGGTFTMGRIDEDVMRDWDNTPRRVTIASFYMDE
                     TEVTNLDYRQYLAWLRYVYPGNSEVYQKALPDTLVWREELAYNEPWVGNYLRHPSYSN
                     YPVVGVSWIQANNYCKWRTDRVNEYLLVENGYLALDSAQRDANTFSTEGYLMNHYQGT
                     PGQYDERIRWENGLLLPNYRLPTEAEWEYAALGLIGEADGELLTQRRMYPWSGTFIRE
                     DQKNAKGQMRANYVRGRGDYMGMAGALNDAADIAGDVFSYAPNDYGIYCMAGNVNEWV
                     ADVYRPLTPFDVEEFQPFRGNVITEYRRDAQGNVLFNDLGQPVLDTVADYRNYGDGDY
                     RSLTDQSKWLQKQDSISTNYMYVQSKSAGVISS"
     gene            1528..>4792
                     /gene="porU"
                     /locus_tag="PKJ43_01640"
     CDS             1528..>4792
                     /gene="porU"
                     /locus_tag="PKJ43_01640"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HNZ71290.1"
                     /translation="MRHNILPSLLAFFLLVTHYTTDVYARGLGATDLISEKNGTTYAN
                     TSVLATGKWIKVSTTNRGIVKLSYNKLNSMGISNPSNVAVYSNGGFMLPKMNYLPYPD
                     DLTQLPVIHTKDKSGANCIFFYSPGSTSWSYNSSASTFDHTINLYSDSTFFYLSSDIS
                     PSPAPSGLEVPAENADTTISTYTALAHYEKEEINLINSGRLWFSDLLLSGLRKTYTFS
                     FENAIDGKLASVIVNSGARCSEGSKMNVKANSTNSYSIDYRAVNISSDGADYAYLVKS
                     VLNIPAKSNITLDFTYSAPDNGEGSKAYIDNITLAVPSRLQLSSGQTDFRNADVRNYT
                     TVEYRIENTNNNTIVWNVTSPLEPKQVFSTFNNTNNTTSIKCSGGIVGEYLVFDPVNG
                     NFPEPQFSANVPNQNIHGLPNYDFIIVTHPNFAEQAERLAEFRRTSDNLSVLVVTTTE
                     VFNEFSSGLPDIAAIRNMFRMFYSRNKASATTFKYALLMGDGSYNNRSTDKEKFNNYI
                     PTYQSVGSLTKGSSFVSDDFFGLLDEDEGENSGFIDIGLGRIPCATENQAEIVIDKII
                     KYSSNETLGSWRNTVCLIADDEDGNAYMKDAEEINTILHTNWPGFSSKKIYIDAYKQV
                     ATSGGFSYPDANKEINETINDGALIVNYIGHGNPNAMASENIVVTKDISSWKNSQSLS
                     LFLTATCEFGPFDAYETSAGENVLLNPSGGAVAIFTTTRVVFGDSNQELSKLFYKNIF
                     KHDEMGEKLRLGDVMRNAKASLAKNDENKSKFVLLADPTLRLAFPKFNVCVDKINGVE
                     IDSNRITLGALEKVTIEGSITDHNDNLIPNFNGKVNTTIFDKEQIAQTLANDGGTPFE
                     YPVQNNVIYKGESSVVDGKFQLTFIVPKDISYNIGPGKILFYATDNEHDANGAVTNIN
                     IGGSGNNPITENTPPEVELFMNNEDFKPYDKISSSALLWVNIFDESGINAAGAGIGHD
                     LVAVIDGDYSNPIILNNYFSNISDSYQKGRIIYPIEGLEPGEHKIWVKIWDVQNNSAE
                     KETYFIVDNGFKITSVCNMPNPVSSYTDFVIDHNLPGDIFNIGIDIYTLSGQKIANIS
                     "
BASE COUNT         1517 a         1034 c          899 g         1342 t
ORIGIN      
        1 ttgaactgat tacaccagcg cttttacttt gaacatacat gtagttagtt gaaatagaat
       61 cttgcttttg cagccatttg ctttgatcgg tcagtgaacg ataatcgcca tcaccatagt
      121 tacgataatc ggcaacagtg tcgagaacag gctgtcctaa atcattaaaa agaacattac
      181 cttgagcatc gcgacggtat tctgtaataa catttccacg gaaaggttga aattcttcta
      241 cgtcgaacgg agttaaggga cgataaacat cggcaaccca ttcgttcacg ttacctgcca
      301 tacaatatat accataatca ttgggagcat aagaaaatac atcgccagct atgtcagcag
      361 catcattaag agccccggcc atacccatgt agtcgcctcg tcctctgacg tagtttgccc
      421 tcatctgacc tttggcattt ttctggtctt cacggataaa agtacccgac caaggataca
      481 ttctacgctg tgtaagcaat tcgccatcgg cttcgcctat aagcccaagg gctgcatatt
      541 cccactcagc ttcggtcgga agtcggtagt taggaagcaa taaaccattt tcccaacgaa
      601 tccgttcgtc atattgaccc ggagttccct gataatggtt catcaggtat ccttcagttg
      661 agaaagtgtt ggcatcgcgc tgagcagagt caagggcaag gtatccgttt tctactaaaa
      721 gatattcatt tacacggtca gttctccatt tacagtaatt atttgcctga atccagctta
      781 caccaactac ggggtaatta ctatatgatg gatgtctgag gtaattaccc acccagggtt
      841 cgttgtaagc cagttcttct cgccaaacca aggtgtcggg caatgctttt tgatatactt
      901 cagaatttcc gggatataca tacctcaacc atgccagata ctgcctataa tcgagattgg
      961 ttacctcggt ttcgtccatg tagaaagaag caatagtaac acgacgaggg gtattgtccc
     1021 aatcgcgcat tacatcttcg tcgattctac ccatggtaaa tgttccacct tgaaccaata
     1081 ccaaaccagg accagtctgt tgctctatat aggccgggtc agtaaaacca aacacttcca
     1141 tatcgtttgc ttgccaccct gtagtgcgag aaggctgacc tccggttttt gacagcggac
     1201 ttttcttcgt ctttgaacta ttacaacctg taaagaccag aagcaacatt atcagaatca
     1261 atgtaatttg tctcattttc caaataatta caatgtttag caacaaatat aactaaaatg
     1321 accaatactt attgtattta atcattttct ttagttcaat aaaattcaat ttttttctta
     1381 aataaaacac aaaaataaat tttaaattga aatctactca accatttgtg agaaaacgtt
     1441 gtttcatttt acttattttg atccctacca aataaaattc gtcctacaat attttaattt
     1501 tgaacccgtt ttttaaagaa aaataaaatg agacacaata ttttaccatc cttacttgca
     1561 ttttttctcc tcgtaacaca ttacacaact gatgtttacg caagagggtt gggtgctact
     1621 gaccttatat cagagaagaa tggtacaacg tatgctaata cttctgtttt ggcaacagga
     1681 aaatggatta aagtgtcgac aacaaatagg gggattgtaa aactcagcta caataaatta
     1741 aatagtatgg gcatatcaaa tccatcgaat gtggctgtgt acagtaacgg tggtttcatg
     1801 ctgcccaaaa tgaattattt gccctatcca gatgatttga cgcaactgcc tgttatacat
     1861 acaaaagaca aaagcggagc aaattgtata ttcttttact cgccaggttc aacaagctgg
     1921 agttacaata gttcagcttc gacatttgat catacaatta atctatatag tgattctaca
     1981 tttttctact tatcttctga catttccccc tcaccggcac catcaggcct cgaagttcct
     2041 gcagaaaatg cagacacaac aatttcaaca tacactgcct tagcccatta cgaaaaagaa
     2101 gaaattaacc tgatcaattc aggaaggcta tggttcagcg acttactgct ttctggcttg
     2161 cgtaaaacct atactttctc ttttgaaaat gctatagatg ggaaattggc atcagtgatt
     2221 gtaaattccg gcgcacgctg ctccgaaggt tcaaaaatga acgtcaaagc aaatagtacg
     2281 aattcctatt ctatcgacta ccgggcagta aacataagtt ctgacggagc tgattacgcc
     2341 tatcttgtca aatcggtatt aaacatacca gcaaaaagta atattacgct tgactttacc
     2401 tattctgccc ccgacaatgg cgaaggcagt aaagcttata tcgacaacat aacgcttgcc
     2461 gtaccctcca ggcttcaact ttcatcgggc caaactgatt tcaggaatgc cgatgtaaga
     2521 aattacacaa cagttgaata ccgaattgaa aacaccaata ataatactat tgtgtggaac
     2581 gttacttccc cacttgaacc aaagcaagtt ttttctactt tcaacaacac aaacaacacc
     2641 acatcaataa aatgctcagg tggaattgtt ggcgaatatt tggtgtttga ccctgttaat
     2701 ggcaatttcc ccgaacccca gttctcagca aatgttccaa accaaaatat tcacgggctt
     2761 cctaattatg atttcataat tgttacccat cccaactttg ccgaacaagc cgaaagactg
     2821 gcagaattca ggcgaacttc cgacaatctc agtgttttgg tagttaccac aaccgaagtg
     2881 ttcaacgaat tttcttccgg gttacctgat attgctgcaa tccggaatat gttccggatg
     2941 ttttattctc ggaacaaagc gtcggcaaca acatttaaat acgcactgct tatgggcgac
     3001 ggctcgtata acaaccgcag caccgacaaa gaaaaattca acaactatat cccaacatat
     3061 caatcggtgg gttctttaac caaaggatct tcctttgtta gcgacgattt ctttggactt
     3121 ctggatgagg acgaaggcga aaactcgggg ttcatcgaca tcgggcttgg tcggattccc
     3181 tgtgcaaccg aaaaccaagc tgaaatcgtt atcgataaaa ttattaaata ctcaagtaac
     3241 gagacccttg gatcgtggag aaatacagta tgcctaattg ccgatgatga agatggtaac
     3301 gcatatatga aagatgctga agaaatcaac accattcttc acacgaattg gccaggtttc
     3361 tcgtcaaaaa agatctacat agatgcctat aagcaagtag caacctcagg aggattctct
     3421 taccccgatg caaataaaga aataaacgaa acgattaacg atggcgcact gatcgttaat
     3481 tatattgggc atggaaaccc gaatgccatg gctagcgaaa acattgtagt tactaaagat
     3541 atttcctcgt ggaaaaacag ccagtccctt tctttgtttc ttacagcaac atgcgagttt
     3601 ggacctttcg atgcatacga aacatcggct ggcgaaaatg tattgcttaa cccttcggga
     3661 ggtgctgttg ctattttcac cactacgcga gttgtatttg gcgatagcaa ccaagaactt
     3721 agcaaacttt tctacaaaaa tatttttaaa cacgatgaaa tgggcgaaaa attaaggctt
     3781 ggcgatgtta tgcgaaatgc taaggcatcc ttagccaaaa atgatgaaaa caaatcaaaa
     3841 ttcgttctac ttgctgatcc aacgctacgt ctggctttcc caaaattcaa tgtctgcgtt
     3901 gataaaataa acggtgttga aatagattca aacaggatca cccttggcgc actcgaaaaa
     3961 gtgactattg aaggctcgat aaccgaccac aatgacaatc tgatcccaaa ttttaatggg
     4021 aaagtaaaca caacaatttt tgataaggaa caaatagccc agactcttgc caacgacgga
     4081 ggaacaccgt tcgaatatcc ggttcagaat aatgtaattt acaaagggga atcatcggtt
     4141 gtagacggta aatttcagtt aacgtttatt gtacccaaag atatatcgta taatattggc
     4201 ccgggaaaaa tattatttta cgccaccgac aacgaacacg atgccaatgg agctgtaacc
     4261 aatatcaata ttggtggttc gggaaacaac ccgataaccg aaaatacccc tcccgaagtt
     4321 gaactcttca tgaataatga agactttaag ccctacgata aaatttcttc gagcgcgttg
     4381 ctttgggtga acatctttga cgaaagtggc attaatgcag ctggagccgg gattgggcac
     4441 gacctggttg ctgtaattga cggcgattat tcaaatccaa taatcttaaa taactatttt
     4501 tcaaacattt cagacagcta tcaaaaagga agaataatat atccaattga aggtcttgaa
     4561 ccgggagaac ataaaatctg ggtgaaaatt tgggatgtgc agaacaattc ggctgaaaaa
     4621 gaaacttact ttattgttga caacgggttt aagatcacat cggtgtgcaa catgcccaat
     4681 ccggtttcgt cgtacaccga ttttgtgatc gaccataatt tgcctggtga cattttcaat
     4741 atcggaattg acatatacac tctgagcgga caaaaaattg ccaacatatc gg
//
LOCUS       DAOPUW010000056         4779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_17919_length_4779_cov_3.328959, whole genome shotgun sequence.
ACCESSION   DAOPUW010000056 DAOPUW010000000
VERSION     DAOPUW010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17919_length_4779_cov_3.328959"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /gene="menD"
                     /locus_tag="PKJ43_01645"
     CDS             <1..1605
                     /gene="menD"
                     /locus_tag="PKJ43_01645"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HNZ71291.1"
                     /translation="ISPGSRNAPLTRLFSANPAFTCHSIVDERSAAFYALGISLASKK
                     PVALVCTSGTAVLNYAPALAEAFYQRVPLIAITADRPAHLIDQQDNQTIHQVDVYHNF
                     IKGSINLAWPLQSEKDLAQQHVEISNIINLAVSGIQGPVQINAPISEPLYEALPQPSP
                     FLKITKNENLPSNNIESSFLETWKNAKRRMVLCGQNTPNEELQKTINLLSKENQAIVL
                     AEPISNMKGENVISPLDRVLMRVEQSELEEFQPDLLVSFGGPVVSKRLKQWLQKQTNL
                     VHWRIAEQEDEIDTYQNRKAHIVGKPAIILAEFAQIGKPSESQFLENWQQTMRQSFAA
                     DTTFFRAAPFCDASVFHFILNDIPTKATLHLGNSSPVRYAQMYTCQEAKAVYSNRGVS
                     GIDGSLSTASGIATISEGLHVVLLGDLSFVYDSNALWNRNFPENLRIVVINNGGGGIF
                     RLLPGPSEQECFEDFMEASHPVQIEKLAQAFGVNYFRAENQQELELQYSTFIEQKGPS
                     LFEIKTPNTENPVQFKAHIQKIKEYI"
     gene            1625..2458
                     /locus_tag="PKJ43_01650"
     CDS             1625..2458
                     /locus_tag="PKJ43_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HNZ71292.1"
                     /translation="MTKTNKIAVLDTEISVSTILGEDYICITDMAKAKSDESRAADII
                     KNWIRTRTTLEFLGTWEKIYNPNFKVVEFDHFKTQAGLPSFVLSPTQWVEKTNAIGIY
                     SKQGRYGGTYAHKDIAFEFGSAISAEFKLYLIKEFQRLKNEENDRQKLDWNLQRTLAK
                     VNYSIHTDAIKEKLIPKKLSAQQISFVYASEADLLNVALFGTTAANWRINNQNLPGNI
                     RDYATLEQLVILSNLESINAVLIRQGLGQTERIQQLNDIAILQMTSLVRSNSIKSLKE
                     K"
     gene            2458..3279
                     /gene="menB"
                     /locus_tag="PKJ43_01655"
     CDS             2458..3279
                     /gene="menB"
                     /locus_tag="PKJ43_01655"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HNZ71293.1"
                     /translation="MATRNWETIKEYEDIFFEYFDGIGKITINREHVRNAFRPTTVNN
                     MSDALRICREDNNINVIVITGKGDKAFCSGGDQNVKGTGGYIDENGIPRLNILEVQKQ
                     IRSMPKPVIAMVNGYAIGGGHVLHVVCDISIASENAIFGQTGPKVGSFDAGLGSSYLA
                     SVVGQKKAREIWFMCRQYSAAEALEMGLVNKVVPLDQLEDEVVAWAQKMQEHSPLALR
                     MLKMGMNADLDGQIGIQEFAGNATLLYYLTEEAQEGKHAFLEKRKPDFKKYPKFP"
     gene            3352..4257
                     /locus_tag="PKJ43_01660"
     CDS             3352..4257
                     /locus_tag="PKJ43_01660"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:NF004750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HNZ71294.1"
                     /translation="MNQIKIWLQAFRLRTLPLALANAVMGSFLAKSTGIFRWDVLILA
                     LSTIVLLQILSNLANDYGDAVSGKDTEKRVGPQRVTHTGMVSMQAMKRMIITFVILSF
                     LTGSALIIVSLSALGWKIITLFFALGIAAIGAALKYTIGKNPYGYKGLGDIFVFIFFG
                     PVGVIGTFYLHTLHYDNMVLVPSAVIGILSTAVLNINNLRDIDSDRETGKQTLAVKRG
                     RLFAQRYHLLLIGSAIVLSVAFTLNYYFAPTQWLFLLTIPLFLRNCIKVYKNENAQTM
                     NNELKNLALTTFLFSVLFGIGLIIK"
     gene            4447..>4779
                     /locus_tag="PKJ43_01665"
     CDS             4447..>4779
                     /locus_tag="PKJ43_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNZ71295.1"
                     /translation="MRTFVTMLILVALSQVISAQNPIVTDQFSADPSARVFNGKVYVF
                     PSHDIPTPPEKPGRKDWFCMEDYHVYSSENLTEWTDHGMIVSQYSAPWVDSTTYSMWA
                     PDCIERNGK"
BASE COUNT         1500 a         1033 c         1001 g         1245 t
ORIGIN      
        1 atttcgccgg gctcacgcaa tgcaccgtta accaggcttt tctcggctaa cccagcattc
       61 acctgccata gcattgtaga tgaacgctct gcggcgtttt atgcattggg tatttcgctg
      121 gcaagcaaaa aacctgtggc cttggtttgc acgtcgggaa cggcggtgct caattatgcg
      181 cccgcactgg ctgaagcctt ttaccagcgt gttccgttaa ttgcaataac ggccgatcgt
      241 ccggcgcact taatcgatca acaagacaat caaaccatcc atcaggttga cgtttaccac
      301 aacttcatca aaggaagcat caatctggct tggcccctgc aaagcgaaaa ggacttggca
      361 caacaacatg tcgaaatttc aaacatcatc aacttggccg taagcggaat tcaaggaccg
      421 gtgcaaatca acgcgcccat ttcagaaccg ctttatgagg cactcccgca accctcaccg
      481 tttctgaaaa tcacgaaaaa tgagaatctt ccatcaaaca atatagaaag tagttttctt
      541 gaaacctgga aaaacgccaa acggcgcatg gttttgtgtg gccaaaacac gcccaacgaa
      601 gaattacaaa aaacaataaa tcttctctcg aaagaaaatc aggctattgt tctggccgaa
      661 cccatttcga acatgaaagg agaaaacgta atttctccgc tcgaccgggt gctgatgcgt
      721 gtagaacaat cagaattgga agaattccaa cccgatttgt tggtttcgtt tggcggccct
      781 gtggtttcga aacgactgaa acaatggttg caaaagcaaa ccaacctagt tcactggcga
      841 attgccgagc aggaagatga aattgacact tatcaaaacc gaaaagcgca catcgtaggc
      901 aaaccagcca ttattttggc tgaatttgca caaattggaa aaccgtcaga aagtcaattt
      961 ctcgagaact ggcaacagac catgcgccaa agctttgccg ccgatacaac ctttttcagg
     1021 gctgccccat tttgcgatgc ttctgttttt catttcattt tgaacgacat accaactaaa
     1081 gccaccttac acttgggcaa cagttcgcca gtgcgatatg cacaaatgta cacctgccaa
     1141 gaagcaaaag cagtgtattc caaccgtggc gtttcgggca ttgatggcag tttgtccacc
     1201 gcaagcggaa ttgcaactat ttctgaaggt ttacatgtgg ttttgcttgg cgacctgagt
     1261 tttgtgtacg attccaacgc cctatggaat cgcaactttc ctgaaaactt gcgtattgta
     1321 gtcataaaca atggtggcgg cggcatattc agactactac cgggtccatc ggagcaagaa
     1381 tgtttcgaag attttatgga agccagtcac cctgttcaaa ttgaaaaact ggcccaagca
     1441 ttcggtgtaa attatttccg ggctgaaaac cagcaagaac tggagcttca atattcaacg
     1501 tttatcgaac agaaagggcc ttcgttattc gaaattaaaa caccgaatac cgaaaacccg
     1561 gttcagttta aggcacatat tcagaaaata aaagaatata tttaactgga taaccatacc
     1621 tgccatgaca aaaacgaata aaatagcagt tctggatact gaaatatcag tgtcaacaat
     1681 cttaggagaa gactacatat gcataaccga tatggcaaaa gctaaatcgg atgaaagtag
     1741 ggctgccgac attattaaaa actggattag aacaagaaca acactcgaat ttttaggtac
     1801 ttgggagaaa atttacaatc caaattttaa agtggtcgaa ttcgaccact ttaaaacaca
     1861 ggcaggttta ccttcttttg tattgagccc aacacaatgg gttgaaaaaa caaatgcaat
     1921 aggaatatac tcaaaacaag gaagatacgg aggaacttat gcacataaag acatcgcgtt
     1981 tgagtttggt tcagcaataa gtgcagaatt caaactttat ttgatcaagg aattccaacg
     2041 gctgaaaaat gaagaaaacg atcggcaaaa acttgattgg aatttacagc gaacattagc
     2101 aaaagtgaat tactctatcc atacagatgc aattaaagaa aaactaatac ctaaaaagct
     2161 ttcagcacaa caaatatctt tcgtttatgc ttcagaagct gatttattga atgtcgcatt
     2221 gttcggaact acagctgcca attggcggat caacaatcaa aatctccctg ggaatatcag
     2281 ggattatgca actcttgaac aattggttat tttgtcgaac cttgaaagta taaatgctgt
     2341 tttaattcgg caaggcttag gtcaaaccga aagaatccag caacttaatg acatagcaat
     2401 actccagatg acatcacttg ttcgaagcaa tagtattaaa agtttaaaag aaaaataatg
     2461 gcaacacgga attgggaaac tattaaagag tacgaagata ttttcttcga atatttcgat
     2521 ggaattggca aaattaccat caaccgcgaa catgtgcgta acgcctttcg tccaaccaca
     2581 gtaaacaaca tgagtgatgc cctgcgaatt tgccgcgagg acaacaacat caacgtaatt
     2641 gtgataacag gcaaaggcga caaagctttt tgctcgggcg gcgaccaaaa cgtaaaaggt
     2701 acaggcggct acatcgacga gaacggtatt ccccgcctca acatccttga ggtgcaaaag
     2761 caaatccgaa gtatgccaaa acccgttatt gccatggtaa atggatacgc cattggcggc
     2821 ggtcacgtgc tgcacgtggt ttgcgacatt agcattgcca gcgagaatgc cattttcgga
     2881 caaacaggcc ccaaagtggg tagcttcgat gccgggttag gttcatcgta cctggccagt
     2941 gtggttggac agaaaaaagc gcgcgaaatt tggttcatgt gccgccaata ctctgctgcc
     3001 gaagctttgg aaatgggctt ggtgaacaaa gtggttccac tcgaccaact ggaagacgaa
     3061 gtggtagcct gggcacaaaa aatgcaggaa cacagtccgt tggcattacg catgttgaaa
     3121 atgggcatga acgccgacct cgacgggcaa attggcatcc aggaatttgc aggaaatgca
     3181 acgcttcttt attacctaac cgaagaggca caagagggaa aacatgcatt tctggaaaaa
     3241 cggaaacccg actttaagaa gtatccaaaa tttccttgat aacagtaaac taagaaacta
     3301 acaagtagtt aattacaagt tagttagatt ttaacattcg aactgaataa tatgaatcaa
     3361 attaagatct ggttacaggc atttcgtttg cgcacgctcc cactggcttt ggccaatgcg
     3421 gtaatgggga gtttcctggc caaaagtaca ggtatatttc gttgggatgt tttgattttg
     3481 gcactttcaa caatcgtttt attgcaaatt ctatccaacc tggcaaacga ctacggcgat
     3541 gccgtttcag gaaaggacac cgaaaaacgg gtagggccac aacgggtaac acataccgga
     3601 atggtctcga tgcaggccat gaaacgaatg atcatcacat ttgtaatcct ttcgttctta
     3661 actggctcag cgcttattat tgtttcgctc agcgctcttg gctggaagat tatcacccta
     3721 ttctttgcac taggaattgc agccattggg gccgcattaa aatacaccat cgggaaaaat
     3781 ccatacggat ataaaggctt aggtgatatt ttcgtcttca ttttttttgg acccgttggt
     3841 gtaattggaa cgttctatct gcacaccttg cattacgata acatggtgct ggtgccctca
     3901 gcagtaattg gcattttaag caccgccgtg ctcaatatca acaacctgag agatattgac
     3961 agcgacaggg aaaccgggaa acaaaccctt gctgtaaagc ggggccgact ttttgcccaa
     4021 cggtatcatt tgcttcttat cggttcggcc attgtgcttt cagtggcgtt cactctgaat
     4081 tattattttg ctccaaccca gtggctcttt ctgctcacga ttccactttt tttgaggaat
     4141 tgcataaaag tttataaaaa cgaaaatgca caaacgatga acaacgaatt gaagaatttg
     4201 gccctgacca ccttcctttt ttcggtgttg tttggaattg gactcataat caaatagaaa
     4261 atcaccttca ttgaaaaaca agccatagtg cttaatacta ataccttaac gaacttattt
     4321 tcaattattt cggataagtt acaattttga attctcaata aatctatttt tagacatgga
     4381 atcttaagta ttcttctatt tttaaggttt aatatcacga aaattgaaat cactaaaatt
     4441 caacgtatga gaacttttgt taccatgtta attcttgtag cgctttctca agtaattagc
     4501 gcccagaatc cgatcgtaac cgatcaattt tctgccgacc catcggcaag agtatttaat
     4561 ggaaaggttt acgtatttcc ttcgcacgac attccaacac ctcccgaaaa accgggtaga
     4621 aaggattggt tctgcatgga agactaccac gtttattcat cggaaaacct taccgaatgg
     4681 actgatcatg ggatgattgt cagccagtac agtgcccctt gggttgattc tacaacgtat
     4741 agtatgtggg cacccgactg catcgaaaga aatgggaaa
//
LOCUS       DAOPUW010000057         4717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_18247_length_4717_cov_3.770914, whole genome shotgun sequence.
ACCESSION   DAOPUW010000057 DAOPUW010000000
VERSION     DAOPUW010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18247_length_4717_cov_3.770914"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..567)
                     /locus_tag="PKJ43_01670"
     CDS             complement(64..567)
                     /locus_tag="PKJ43_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71296.1"
                     /translation="MFKIRDKILSKAINKRWENVYYPDLNAIRTVAYICGEVPSDLER
                     LWQKAGFEVKVTCYSFAEGKRTDDLSWNKLYSSDLNIWKLPKAKPINQFVEKEFDVLI
                     LAEKQPSREALTYVCAVSKAKFKVGSQQAGRLFDLVIGQEQPDAVKITSQLIKILSNL
                     KPAIKMN"
     gene            816..1667
                     /locus_tag="PKJ43_01675"
     CDS             816..1667
                     /locus_tag="PKJ43_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ71297.1"
                     /translation="MTKHKSSNILINKASGEQEFFDVAKLRNSLSRAGADDFVVKDIT
                     ENIQAWLYEGVSTKKIYERAFTLLRRQSQRNATLYKLKRSLFELGPSGYPFEHLIGEI
                     YRRRGFSVEVGVVVQGHCITHEMDVIATNTSEQILLECKYGIDQGKTISIQVPLYVRS
                     RIEDIVAERKQIPQYKDLTFNGGVVTNTRFSADSIEYSKCYGLSLLGWDYPTGNGLKE
                     ILERERIFPVTILSNFTSKQKNFIIDQGIVTCLQLYNDLKVLDSLMFSPNKIKSIREE
                     LEAILEL"
     gene            1779..3500
                     /locus_tag="PKJ43_01680"
     CDS             1779..3500
                     /locus_tag="PKJ43_01680"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNZ71298.1"
                     /translation="MRFYTIMLAALMLSGCHNRTKTGPTPLATVCNPVNISYRFCIDE
                     PSRREAADPSVVWFNGRYFLFASKSGGYWHSKDLGSWEFIETEQIPAEEYAPTVVAIG
                     EELYFLASSNEKSTIYKTSDPLSGQWVVAVEELETPVWDPMFFFENDKLYLYWGCSNT
                     NPLYGVEVDYRNNFKFLGKPVELLKSNTAQNGWEVPGDYNTMTHQSPWIEGAWVNKIN
                     GKYYLQYAGPGTEFKSYSDGVYTSDNPLGPFSLQQHNPFAYRPEGFIAGAGHGSTFSD
                     QYGNLWHIGTLTISQKHMFERRLGIFPVFVSNDGILYTTTRYGDYPYFIPQKKISSYE
                     DISTGWMLLSFRKPVEVSSAIDSLPAVNITDENIRTYWAASGTNSGEYVIIDLQNPTE
                     VKALQVNFAEHNTNIFGRKKGIKYRYVIDCSDDKTTWKVITDRSKNETDNSHDYIQLA
                     NAVTCRYVRITALEVADGNFALSDFRIFGLGNGQKPAIVKSFTAQRNSNDRRSVTLNW
                     EKSATATGYNIRYGTASDKRYLDYMVYGDTSITINSLNTNSKYFFTIDSFNENGISLG
                     EITVQID"
     gene            3671..4648
                     /locus_tag="PKJ43_01685"
     CDS             3671..4648
                     /locus_tag="PKJ43_01685"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HNZ71299.1"
                     /translation="MGNKVKIGIVGYGNLGKGVEMAIKQNSDMELIAVFTRRTPETIN
                     SASKMVSILQIEEYKGKIDVMILCGGSSTDLPEQGAVISAMFNTVDSFDTHAKIPEYF
                     SKVDESSRKNNTLSLISTGWDPGLFSLARLLGQSILPDGKDYTFWGKGVSQGHSDAIR
                     RVKGVKNGIQYTIPIESALEKIRKGSTANFTAAEKHERLCYVVAEENADKAEIEKTIK
                     NMPYYFDEYNTTVNFISEEELKANHSGMPHGGVVFRTGNTGNGTSQRIEFSLNLGSNP
                     EFTASILVAYARAIARMSKEGQTGARTVFDIAPAYLSAKNAEELRRDLL"
BASE COUNT         1550 a         1025 c          920 g         1222 t
ORIGIN      
        1 ttagttcaaa attacggtaa aaataacttt ctctctcgta tgtcaaaact gagccatgta
       61 tttttagttc atctttatgg caggcttaag attcgagaga atttttatga gttgagaggt
      121 aattttcacg gcatcgggct gctcctgccc tatcaccaga tcaaaaagcc tccccgcctg
      181 ttgagaacct actttaaact ttgctttcga aacagcacaa acataggtta aagcctcacg
      241 ggatggttgt ttttcagcta aaatcaatac atcaaattct ttttcaacaa attggtttat
      301 aggcttggct tttggtaatt tccaaatgtt taagtccgat gaatagagtt tattccaact
      361 taagtcgtcg gtcctttttc cttctgcaaa tgaataacag gtaactttca cctcaaaacc
      421 agctttttgc catagccttt ccagatccga tgggacttca ccacaaatgt aagccaccgt
      481 acggattgca ttcaagtcgg gataataaac gttttcccaa cgcttattta tagcttttga
      541 caatatttta tctctgattt taaacatttt aaaacaccgt ttttaaaatt taacccgttg
      601 gataacaatc atgaagttta gcaaaggtag aaaaagataa gcagaaaatt catccccgaa
      661 gaaccctgaa ccaaacagca acagctaaca aaaatataaa aggaacgtga aagcacaaat
      721 ccattttcat tgtacactat ctgtctttat tgtaaaactg tcgttgcaag cttcagcatt
      781 tagttgtatt ttcaaccgtg ataattcaac tcaaaatgac aaagcataaa tcaagtaaca
      841 ttctcattaa taaggcatct ggagagcaag agtttttcga tgttgccaaa ctcaggaatt
      901 cactttcacg tgccggagcc gacgattttg tagtaaaaga tattactgaa aacatccaag
      961 catggcttta tgaaggtgta tctactaaaa aaatatatga acgggcattc acactgctcc
     1021 gtcgtcaaag tcagcgaaat gcgacccttt acaaactaaa acgatctctt ttcgaacttg
     1081 gtccatcggg ctaccctttc gaacatttaa ttggcgaaat ataccgtcgc agaggttttt
     1141 ctgttgaagt tggtgtggtg gttcaaggtc actgcataac ccacgaaatg gacgtaattg
     1201 ctaccaatac cagtgaacaa attttgttgg aatgcaaata tggaatcgac cagggcaaaa
     1261 ccatcagtat ccaagtaccc ctttatgttc gatcacgtat cgaagacatt gttgctgaac
     1321 gaaagcaaat tccacaatac aaagacctga cattcaacgg cggagtcgtt accaacaccc
     1381 gcttttcggc cgactccatt gaatacagta aatgctacgg actttcgtta ctaggctggg
     1441 attaccccac aggaaacgga ctaaaagaaa ttttagaacg cgagcggatt ttcccggtca
     1501 cgattctatc gaactttacc tcaaaacaaa agaactttat tatagatcaa ggaattgtaa
     1561 catgcctgca attatacaac gacctgaaag ttttagacag tttgatgttt agtcctaata
     1621 aaataaaaag tatacgtgaa gaacttgaag ctatactaga attataaaca cgaaacgaaa
     1681 tatcttacat tatatcagat taatacttca acgaaatatt aaaattcaac agtaaaaaac
     1741 agaaaaacac gatttatcaa tctataaaat cagaagaaat gagattctac accattatgt
     1801 tggccgcatt gatgctatca ggatgccata accgcacgaa gacaggacct acacctctcg
     1861 ctactgtttg taatccggta aatatcagct accggttctg tatcgatgaa ccttcaaggc
     1921 gtgaagctgc cgatccgtct gttgtatggt tcaatggacg ctacttcctt tttgcctcaa
     1981 aatcgggtgg atactggcac tctaaagacc taggcagttg ggagttcatt gaaacagaac
     2041 agattccggc cgaagagtat gcccctacgg tagttgcaat tggtgaagaa ttatactttc
     2101 ttgcctcatc gaacgagaaa agtaccattt ataaaacatc agatccactt agcggacaat
     2161 gggttgtagc cgtagaagag cttgaaacac cagtctggga tccgatgttt ttcttcgaaa
     2221 acgacaaact ctacctgtac tggggctgct ccaataccaa cccgctctac ggagtggaag
     2281 ttgactatcg caacaacttc aagtttctgg gaaaacccgt tgaacttttg aaatcgaata
     2341 ctgcgcaaaa cggttgggaa gtacccggcg attacaacac catgactcat cagagtccat
     2401 ggattgaagg cgcttgggta aacaaaatca atggtaaata ttacctgcaa tatgctggtc
     2461 cagggaccga attcaaaagc tatagcgatg gggtctacac atccgacaat ccattgggac
     2521 cattcagttt gcaacaacat aaccccttcg cctaccgccc cgaagggttc atcgccggtg
     2581 caggtcatgg cagtacattt agcgaccaat acggtaacct ttggcacatt ggcactctaa
     2641 ccatatcgca aaaacacatg ttcgaacgga gactgggaat attccccgtc tttgtcagca
     2701 acgatggaat actctacaca acaacacgtt acggagatta tccatatttt attccacaga
     2761 aaaagattag cagttacgaa gatatttcaa ccggatggat gcttctctca ttccgaaaac
     2821 cagttgaagt ttcctccgca attgattcgc tccctgctgt aaatatcacc gatgaaaata
     2881 tccgcaccta ctgggccgct tcgggcacaa acagcggcga atatgtcata atcgatcttc
     2941 aaaacccaac ggaagtaaaa gcgcttcaag ttaattttgc cgaacataac accaacattt
     3001 tcggtcgaaa gaaaggaatt aaatatcgct atgtcattga ttgttcagat gataagacaa
     3061 cctggaaagt aatcaccgat cgatcgaaga acgaaaccga caattcacac gattatatcc
     3121 aacttgccaa tgcagtaact tgccgctacg tacgaataac cgcactcgaa gttgctgacg
     3181 gtaattttgc ccttagcgac ttccggatat ttggtctcgg caacggacaa aagccagcca
     3241 ttgttaagtc atttacagca caacgaaaca gcaacgaccg acgaagcgtt accttgaact
     3301 gggaaaaatc ggccacagct acgggctata acatcagata cggaacagcc agcgataaac
     3361 gctatctcga ttacatggta tacggcgaca caagcataac tatcaacagt ttaaatacaa
     3421 acagcaaata tttcttcacc atcgattctt tcaatgaaaa tggaatcagc cttggagaaa
     3481 taacagttca aattgattga tataaccgtt taagcaagat gtaatacagc gtggtattat
     3541 tcttttatga aatatgattg cagaaaaaga gacaagaaaa atgcagacct gttttttgct
     3601 gatatttgcc cctgagaaaa ctctttcacg tcatttttcg ggtaggaata atatataaca
     3661 cacaacttat atgggaaaca aggtaaaaat tggcattgtt ggctacggaa acttaggaaa
     3721 aggcgtagaa atggccataa agcaaaatag cgatatggaa ttgatcgctg tttttacccg
     3781 gcgtacaccg gaaacaataa acagtgcatc aaaaatggta tctatcttac aaatagaaga
     3841 atacaaagga aaaatagacg taatgatcct ttgcgggggc tcttcgaccg atttaccgga
     3901 acaaggagca gtaatatcgg ccatgttcaa cactgtcgac agtttcgata ctcatgcaaa
     3961 aataccagaa tacttcagca aagttgatga atcgtcgcga aagaacaaca cactgagtct
     4021 catctcgaca ggctgggacc cagggctttt ctcgcttgcc aggcttctgg ggcaaagtat
     4081 attacccgat ggtaaagact acacattctg gggcaaagga gttagccaag gccactcaga
     4141 tgccatacgc cgtgtaaaag gggtaaaaaa cggcattcaa tatacaattc caatcgaaag
     4201 tgcactcgaa aaaatcagga aaggcagtac cgccaacttc actgccgccg aaaagcacga
     4261 aaggctatgc tatgttgttg ctgaagaaaa cgctgacaaa gccgagattg agaaaaccat
     4321 caaaaacatg ccttactatt tcgatgagta taatactacg gtaaatttta tcagcgaaga
     4381 agaactaaaa gccaatcata gtgggatgcc ccacggtgga gtggttttcc gtacaggaaa
     4441 cacaggcaat ggtaccagtc agcgcattga atttagtctg aacctgggca gcaaccctga
     4501 gtttacggcc agcattcttg tagcctatgc acgtgctatt gcccgcatga gcaaagaagg
     4561 acaaaccgga gcccgtactg ttttcgatat tgcccctgcc tacctttcgg caaaaaatgc
     4621 agaagaattg cgacgcgacc tgctctaatc aaaaaaacaa tcatcaaata catggttgat
     4681 cggaataatg cttccggtca accttttttt tgcagaa
//
LOCUS       DAOPUW010000058         4704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_18318_length_4704_cov_2.935040, whole genome shotgun sequence.
ACCESSION   DAOPUW010000058 DAOPUW010000000
VERSION     DAOPUW010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18318_length_4704_cov_2.935040"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..976
                     /locus_tag="PKJ43_01690"
     CDS             233..976
                     /locus_tag="PKJ43_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71300.1"
                     /translation="MNLRSVAFLFLLFFSGSLSAQKQINVLTTNSWTTAYARAAGVTF
                     VDQLAPSDMMHPSEYELQISDIKKLKEADYIIYSGYEVVVPQIQKSLKIDERKLIKIE
                     TGYTEELMIREIEKIARIVGTIKFAQQSATQLSLLFARSRFEIENAGLKGKPVIVHFF
                     QEAFAREIGLKPIAVFGPSHPELHELAAISEKEAVLIIDNVHNQVAQPISAMKKSVKV
                     VSFLNFPGTHDTQTLEDVILYNLSQLLPD"
     gene            1004..1783
                     /gene="truA"
                     /locus_tag="PKJ43_01695"
     CDS             1004..1783
                     /gene="truA"
                     /locus_tag="PKJ43_01695"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNZ71301.1"
                     /translation="MEEQVQQHRYFLQMAYNGAPYHGWQLQPNAVTVQEVLQHAISTL
                     TRENTEVIGAGRTDTGVHASFFVAHFNLTNPLENTDHFARKLNAFLSDDILIVTIFPV
                     STDMHARFSATSRTYRYYVSEQKEPFRKGFVLKINQALDVERMNQAAQLLLQTNDFTS
                     FSKLHTDVNNNLCKVTSARWSREGTLLIFEITANRFLRNMVRAIVGTLIDVGKGKLSA
                     DGFWNTIQVKNRCEAGTSVFGGALFLTDIQYPEPVNSILVR"
     gene            complement(1918..3708)
                     /locus_tag="PKJ43_01700"
     CDS             complement(1918..3708)
                     /locus_tag="PKJ43_01700"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNZ71302.1"
                     /translation="MKIKYSLLILLLTSLFIVSCDEEAFLDKKPHTPTDYGFYTTEDG
                     AVQGVNAAYDILQKGEELEARLEFLGTVCSGDAVAGGEPTGADQPPMQAIAKFMNDPT
                     NNYLVVYWNAMYRGIYRSNLLLQYLKDPIEGFDESLRNRLIGEATFLRALFHFKLQIN
                     FGGFPQLQGNFGNGLMGVPYIDKVLLQEEWNQERPTLEYTWGRIEEDFAKAATLLPDR
                     YTGIDLGRATSGAAKSMLAKTYLYQEKWQDAYNTAKEVINSNRYYLIGEDGHNEPKVI
                     TRTGRNGEFQATVSGYKWIWQPEANNCDESIFDVQHFMDGGNSFLTQEGNIIPRYYGP
                     RQVFAMAWDNQTKKWAETPTEYFWGFILPTRYYVETAYHAVGGVDNGKIVDPRFKLTV
                     VTPEDKVPFTYPDSVRQRFADSVYVNGFYSPYATGYVTWKYFTDPIYNDVRKSLGDMP
                     QNTKYFRFADLLLMGAEAAVHTGNNGDALTWINRVRNRARNAGNTGFPKALSAVSLDD
                     IYAERRVELSFEGHQFYDIVRTGRAEKVLKNEAMQYATNTHQISKVGDLVTTVEQFGD
                     AFDIGKDEICPIPQAEIDNTNGSIKQNPNW"
     gene            complement(3735..>4704)
                     /locus_tag="PKJ43_01705"
     CDS             complement(3735..>4704)
                     /locus_tag="PKJ43_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ71303.1"
                     /translation="SGIQTPPTTNAGSVSNKGWELNVSYRERSGEFKYELGFNIGHVK
                     NELTKLGKDQPINSASFRAIDYVSRSEVGMPIAYFYGYVCDGLYQNADEIEKYNQIAR
                     EATGKPKQVYDNKKAMPGDIIYKDISGPDGKPDGIITSADRRFIGSPHPDLTYGVNVK
                     FEYKNFDLSVFGQGVYGNEVFMATTFYLMSGDAYWNLLNSMKDHWKQDGDKTEIPRLG
                     LSDNNMRLSDRYVKDGSFFRIKNIQLGYTLPQNLSKKAGIERAHIYVSGQNLFSFHNY
                     EGFDPEIGTGRSQGSDVSQRGMLDIGIDRGMYPLAKTMSVGLNLTF"
BASE COUNT         1232 a          994 c         1010 g         1468 t
ORIGIN      
        1 cattgcaact ttgcacgccc gaagctttaa gaaagtagtg ctttgggctt cgctttgggg
       61 cggaattttt tctatttcag gcttttttgc tgcgtattgg cttaaggttc ccaccagttc
      121 ggcgatcgca attctggcca cattggtttt tcttgtcatc tatctgctta gaaaagtgac
      181 attaaaacgg cgcttgcata atattaagtg tttatagtta agtgtttatt gtatgaatct
      241 acgatctgtt gcttttcttt ttcttctttt cttttcgggg tctttgtccg ctcaaaagca
      301 aataaatgtg ttgactacca acagttggac gactgcgtat gcccgtgctg ccggagttac
      361 ttttgtggat cagttggctc cttctgatat gatgcaccct tcggaatatg aattacaaat
      421 cagcgatata aaaaagttaa aagaagccga ttatatcatt tattcgggtt acgaggttgt
      481 tgtccctcaa atacaaaaat cacttaaaat agatgagcga aagcttatta aaattgaaac
      541 cggttatacc gaagagttaa tgattcgcga gattgagaaa atagcccgaa ttgtaggaac
      601 cattaaattt gcccaacaat cggctaccca acttagtctg ttgttcgcaa ggtcccggtt
      661 cgagatagaa aacgcgggtt tgaagggcaa accggtgatt gtccattttt tccaagaagc
      721 ttttgctcgt gaaattgggt tgaagcctat tgctgttttt ggcccatctc atcctgaatt
      781 acatgagttg gctgcaattt cagaaaaaga agctgttctc attattgata atgttcataa
      841 tcaggttgct caacctattt cggccatgaa aaaaagcgtt aaagttgtca gttttcttaa
      901 tttccccgga acgcacgata ctcaaacgct cgaagatgtt attctgtaca atctttcgca
      961 acttttgccc gattgattgt tttcctttct atttttgcac tgtatggaag aacaggttca
     1021 acaacacagg tattttctgc agatggccta taacggtgca ccgtatcatg gctggcagct
     1081 tcaacccaac gcagttactg ttcaagaggt gcttcaacat gctatttcaa cacttacgcg
     1141 cgaaaatacc gaagttattg gcgctggcag aactgatacc ggagttcatg cttcgttctt
     1201 tgtggctcac ttcaatttga caaatccgtt agaaaacaca gaccactttg cgcggaaact
     1261 taacgctttc ctgtccgatg atattctcat tgttactatt ttccctgttt ccaccgatat
     1321 gcatgcccgc tttagtgcta cttcgcgtac ctatcgctat tatgtgagtg aacagaaaga
     1381 accatttcgt aaaggatttg tattgaagat taatcaagcc ctcgatgtgg aacgaatgaa
     1441 ccaggcagcg cagctccttt tacaaaccaa tgatttcact tcgtttagta aattgcatac
     1501 cgatgtgaat aataacttat gtaaggtaac cagtgcccgg tggagcaggg aagggactct
     1561 gctgatattt gagattactg caaaccgctt tcttcgcaac atggtaaggg ccatcgtagg
     1621 cacacttatc gatgtgggaa aaggaaaact ttcagccgat ggtttttgga acacaattca
     1681 ggttaaaaat cgttgcgaag cgggtacttc tgtttttgga ggggcgttgt tccttaccga
     1741 cattcaatat cctgagccag tcaattctat attagtgcga tagccgttta atcagtttac
     1801 ttaagttcct gattacaatt gaagttctgc ttttacacat atttctaaga agttttataa
     1861 aaaaaggctg ccccaccaaa gtgaaacagc cttgaaaaat ctatcgataa tacattgtta
     1921 ccagttaggg ttctgtttga ttgatccgtt ggtgttgtcg atttctgctt gaggaattgg
     1981 gcaaatttcg tcttttccta tgtcgaatgc atcgccaaac tgttcaactg ttgttaccag
     2041 atctccaact ttagatattt ggtgggtatt ggtagcatat tgcatagctt catttttcag
     2101 gactttttcg gcacggccag tacggacaat gtcgtagaac tgatgtcctt caaatgaaag
     2161 ttcaacgcgg cgttctgcat agatatcatc cagcgaaaca gctgaaagtg cttttggaaa
     2221 gccagtgttt cctgcattac gggctctgtt gcgtacgcgg ttaatccaag taagagcatc
     2281 tccattatta cctgtgtgaa ctgcagcttc ggcacccatc agcaataaat cggcaaagcg
     2341 gaaatatttt gtattctgcg gcatatcacc aagtgattta cgaacatcgt tgtaaatagg
     2401 atcggtaaag tatttccagg ttacataacc cgttgcgtat gggctataga aaccatttac
     2461 atacacagag tcggcaaaac gctggcgtac agagtcggga tacgtgaatg gtaccttgtc
     2521 ttcaggtgtt actacagtca acttgaagcg tgggtcaaca attttcccat tgtcgacacc
     2581 gcctacagca tgataagcag tctcaacata ataacgggtg ggaagaataa agccccagaa
     2641 atattcagtt ggggtttcag cccatttttt tgtctgatta tcccatgcca ttgcaaatac
     2701 ctgacggggt ccgtagtacc tagggattat gttgccttcc tgtgtgagaa aactattgcc
     2761 gccatccata aagtgttgca catcgaaaat gctttcatcg cagttgttgg cttcgggttg
     2821 ccatatccat ttgtaccctg atacagttgc ctgaaattct ccgtttcgtc cggtacgggt
     2881 aattactttt ggttcgttat gcccatcttc gccaataaga tagtaacggt ttgaattgat
     2941 tacttctttt gcagtattat aagcatcttg ccatttttcc tgataaaggt aagtttttgc
     3001 cagcatcgat ttagcagcac ctgaagttgc tcgtcctagg tctataccgg tgtaacgatc
     3061 gggaagtaaa gttgcagctt ttgcaaagtc ttcttcaatt cgtccccatg tgtactccag
     3121 agttgggcgt tcctgattcc attcttcttg caatagtact ttgtcgatat agggaacccc
     3181 cataagtccg ttgccaaaat tgccctgaag ctgaggaaat ccaccaaagt tgatttgtaa
     3241 cttaaaatgg aacaatgccc ttaggaaagt ggcttcacca atcagtctgt ttctaaggct
     3301 ttcatcaaat ccttcaatag gatctttcag gtattggagc aacaggttac ttcggtagat
     3361 accacggtac atagcattcc agtacacaac aaggtagttg ttggtagggt cattcataaa
     3421 tttagcaatt gcttgcattg gaggctggtc ggctccggtt ggttcaccgc cggcaactgc
     3481 atcgcctgaa caaacagtac ctaagaattc caaacgggct tccagttctt cacctttctg
     3541 aagaatatcg tatgcggcat taacgccttg aacggcgcca tcctctgtgg tatagaagcc
     3601 atagtcggtg ggcgtgtgcg gttttttatc caggaacgct tcttcatcgc atgaaacgat
     3661 aaataaagaa gtcagcagaa ggattaatag tgaatatttt attttcatcg tttttaaaat
     3721 ttacggttag ctgattagaa tgtaaggttt aaaccaacag acattgtttt tgcaagcggg
     3781 tacataccgc gatcaattcc tatgtcgagc ataccgcgtt ggcttacgtc cgatccttgt
     3841 gaacgaccgg taccaatttc agggtcaaat ccttcgtagt tatggaacga aaatagattc
     3901 tggccgctta cgtagatatg agccctttca attcctgctt ttttcgacag gttttgtgga
     3961 agggtgtaac cgagttggat gttcttgata cggaagaaac ttccgtcttt tacataccgg
     4021 tctgataagc gcatgttgtt atccgacaac cctaaacgag gtatctcagt tttgtcgcca
     4081 tcttgtttcc agtggtcttt catcgagtta agcaaattcc agtatgcgtc gccactcatc
     4141 aggtagaagg tggtagccat aaatacttcg ttcccgtaaa ctccttgtcc gaaaaccgat
     4201 aagtcgaaat tcttatattc aaatttcacg ttcaccccat aggtaagatc ggggtgcggg
     4261 cttccaataa acctacggtc agcactggtg ataataccgt cgggtttgcc atcgggacca
     4321 ctaatatctt tgtagataat gtcaccgggc atggccttct tgttgtcgta cacttgtttt
     4381 ggcttgcctg tggcttcacg ggctatttgg ttgtattttt caatttcatc ggcgttttga
     4441 tacaagccat cacaaacgta tccatagaaa taggcaatag gcattcccac ttctgatctc
     4501 gatacgtagt ctatggctct gaacgatgct gaattgatag gctggtcttt accaagcttt
     4561 gtgagctcgt ttttaacatg tccaatgttg aaacccagtt cgtatttaaa ctcacctgaa
     4621 cgttcacgat acgatacatt aagctcccat cctttgttcg aaacagatcc cgcattggta
     4681 gttggagggg tttgtatgcc ggaa
//
LOCUS       DAOPUW010000059         4652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_18571_length_4652_cov_3.749837, whole genome shotgun sequence.
ACCESSION   DAOPUW010000059 DAOPUW010000000
VERSION     DAOPUW010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18571_length_4652_cov_3.749837"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PKJ43_01710"
     CDS             <1..447
                     /locus_tag="PKJ43_01710"
                     /inference="COORDINATES: protein motif:HMM:NF020052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HNZ71304.1"
                     /translation="KIAALQIICSRPKELDRKSLKEFYLLLDEQGYNTRSLNSAWKDA
                     KNENIAADIISFIRTLALGDTLISHEERIKKAVQKVRDSRTWNTIQLKWLEKFEKQLL
                     VENVLRADDLNEDPFKGDGGFNRLNKIFENELEKVIELINEHLYTA"
     gene            558..1016
                     /gene="tnpA"
                     /locus_tag="PKJ43_01715"
     CDS             558..1016
                     /gene="tnpA"
                     /locus_tag="PKJ43_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HNZ71305.1"
                     /translation="MANTYTQIYLQLVFSPLGRDNLIVKSIKEELQKYTTGIIQKRKH
                     KLLTINYMPDHVHIFIGYQPTQSLPDLLRDIKSNTSHFINEKKWMPGRFQWQEGYGAF
                     SYSRSHIDDVIKYINNQEDHHKRLTFKEEYLNLLQKFEVEYDEKYLFEWI"
     gene            1999..3459
                     /locus_tag="PKJ43_01720"
     CDS             1999..3459
                     /locus_tag="PKJ43_01720"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNZ71306.1"
                     /translation="MGAEQIAAKLWNLCNVLRDDGVTYHQYLNELTFILFIKLSEIKG
                     FEDQLPEEYRWSKLKNIKDNKELFDTYRELLATVSSKSDNVNIREIFTNANTTLRKPV
                     NLRTLVSAIDELDWYEEHDRDMMGDIYEDLLERNAGEKKSGAGQYFTPRPLINVMVEL
                     VAPKLGERWNDPACGTFGFPISINQYLLHKYDYKYSVSDKVRSFQENEALSGVELVQD
                     AHRLALMNAKLHGLDSTIYLNDTLTEFGKGLKGFDGVLANPPFGTKKGGERPTRDDFT
                     YPTSNKQLNFLQHIYRSLHKKGGARAAVVLPDNVLFEDGDGAKIRRDLMDKCNLHTIL
                     RLPTGIFYAQGVKTNVLFFERATTETGNTQQIWFYDMRTNMPTYGKRTPFNRSAFDGF
                     IKAYTGGITTDKVQLEYDGTIDQTKRAAITDERWQCITREQIAAKNDSLDLGLIADSS
                     LNNGDALADPIDIAKETANELKSILGELEMIIQELK"
     gene            3470..4306
                     /gene="dinD"
                     /locus_tag="PKJ43_01725"
     CDS             3470..4306
                     /gene="dinD"
                     /locus_tag="PKJ43_01725"
                     /inference="COORDINATES: protein motif:HMM:NF008573.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HNZ71307.1"
                     /translation="MKSDEIKNLFFQFEAAASELEGVECWSAREIQNLLGYSKWENFE
                     KVIQKAKEACKTAGEEIDYHFPDVRKMIALGKGAEKEIDDILLTRYACYLVAQNGDSR
                     KEEIAFAQNYFAVQTRRAELVEQRILEFERVKAREKLSQTEKQLSGILYERGVDSQGF
                     AVIRSKGDQALFRLNTQMLKRKMGIPESRPVADFLPTISIKAKDLAAEMTGLNVQSKD
                     LKGQTKIEKEHVDNNLAVRNMLTQRGIIPENLPPGEDVKKLQRKLDGDEKKLLKGTKK
                     KK"
     gene            4440..>4652
                     /locus_tag="PKJ43_01730"
     CDS             4440..>4652
                     /locus_tag="PKJ43_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71308.1"
                     /translation="MENILPKGWVAVQLIELLKVLENGSRPIGGVQGILEGIPSLGGE
                     HLNSDGGFKFETIKYVPIDFAEKMQRG"
BASE COUNT         1532 a          924 c         1024 g         1172 t
ORIGIN      
        1 aaaatcgcag ccctgcaaat catctgttcc cggcccaaag agctcgaccg gaaatcacta
       61 aaggaatttt acctgttgct agacgaacag ggttacaaca cccgttccct gaactctgcc
      121 tggaaagatg ccaaaaatga aaacatagct gccgatatca tctcctttat ccgtaccctg
      181 gcattgggcg atacattgat tagccatgaa gagcggatta aaaaagcagt tcagaaggta
      241 cgcgattcaa gaacatggaa caccatacag ttgaaatggt tagaaaagtt tgaaaaacaa
      301 ctgcttgttg aaaatgtact ccgagctgat gatctgaatg aagacccatt caaaggggat
      361 ggtggcttta accgactgaa taaaatcttt gaaaacgagc tcgagaaggt gattgagctg
      421 attaatgaac acttatatac agcataataa ataacaaaag gaattgaacc cgttagggtt
      481 tttctgtttg tagtatgcaa caaccaacaa aatattagct ccgtcaggag cgacctgtaa
      541 tttaaaactt actaactatg gcaaacacct atacacaaat ctatcttcag ttggtttttt
      601 cacctcttgg ccgtgataat ttgattgtta aaagtataaa agaggaactc cagaaatata
      661 ctacagggat tattcagaaa cgaaagcata agttattaac gattaattac atgcccgacc
      721 atgttcatat ttttatagga taccagccaa cgcaatcttt acctgattta ttaagagata
      781 ttaaatcaaa cacatcgcat tttataaacg aaaagaaatg gatgccaggc cgttttcaat
      841 ggcaagaagg ctatggcgcg ttttcttact cccgctcaca tattgatgat gttataaaat
      901 acattaacaa tcaggaggat catcacaaga gattaacctt taaagaggag tatcttaatt
      961 tattgcagaa gtttgaggtt gaatatgatg aaaagtattt gtttgagtgg atataacgaa
     1021 caggtcgtcc cgacgggact ccgttgctgt ttttacctgt attactacaa acaggaaaca
     1081 cctacggtgt cgtatttcat tatcaaagca ggttgaaaca aacaaaagct attgttatgg
     1141 gattttatca aacccatagg gttaatctgt ttgtagaaag ataggatttg tattgcacag
     1201 gtttttatca aacccgtagg gttgacctgt ttgtagataa aaaggattgc atagatttaa
     1261 agctccgtag gagcgacctg ttttaataaa ccagcaacga acaggtcgtc cctatgggac
     1321 tccgtttctg gtttcgaccg tattactaca aacaggaaac acctacggtg tcgtatttca
     1381 ttatcaaaac gggttgaaac aaacaaaagc tattgtactg ggatttttaa acccgtaggg
     1441 ttaatctgtt tgtagaaaga taggatttgt attgcacagg tttttatcaa acccgtaggg
     1501 ttgacctgtt tgtagataaa aaggattgca tagatttaaa gctccgtagg agcgacctgt
     1561 tttaataaac cagcaacgaa caggtcgtcc ctatgggact ccgtttctgg tttcgaccgt
     1621 attactacaa acaggaaaca cctacggtgt cgtatttcat tatcaaaacg ggttgaaaca
     1681 aacaaaagct attgctatga gatttttaaa cacataggtt tgatctgatt aaaaaaatga
     1741 aagaataaaa aagtcaaccg ttggcttacc aatttgaaat gaatattttt gtccctaagg
     1801 cactacgtca ccatgaaaag taaagtggca actttaaatc cgtgaagcct tgaaatgtct
     1861 ttttcagaaa aataacaaat acaaaccttc taccttaaac ggtaggataa agtccccgga
     1921 atttatcccg cagaaagaga gaggggattt aggggttaaa taacaaattg tcattcataa
     1981 ataataaatt ttttcaccgt gggagcagaa caaatcgcag caaaattatg gaacctctgt
     2041 aacgtccttc gcgacgacgg ggttacctat caccaatacc tgaatgaact tactttcatt
     2101 cttttcatta aactgagtga aataaagggt tttgaagatc agttgcctga agaatacagg
     2161 tggagtaagc tcaaaaatat taaagacaac aaagagttgt tcgatactta ccgggaactg
     2221 ttggctaccg tgagcagcaa atcggacaat gtgaacatcc gcgagatttt caccaatgcc
     2281 aataccacct tgagaaaacc ggtgaacctg agaacgctgg tctctgccat tgatgagctg
     2341 gactggtacg aggaacacga ccgcgatatg atgggcgaca tctatgaaga tctgcttgaa
     2401 cgcaatgcag gtgaaaagaa aagtggtgcc gggcaatatt ttactccccg tccgcttatt
     2461 aacgtgatgg ttgaactggt tgctcccaaa ctgggagaac gttggaacga tccggcctgt
     2521 ggtacctttg gattccccat ttccataaac caatacctct tgcacaagta cgactacaaa
     2581 tacagcgtaa gcgataaagt caggtctttt caggaaaacg aagcacttag cggggttgaa
     2641 ctggtacagg atgcccaccg tttggcgttg atgaatgcca agctgcacgg tctcgacagc
     2701 accatttacc tgaacgacac cttgactgag tttggcaaag gcttgaaagg gttcgacggt
     2761 gtgcttgcaa atcctccatt tggaaccaaa aaaggaggtg aacgacctac ccgcgacgat
     2821 tttacctacc ctaccagcaa caagcagctt aactttttac agcacatata ccgctcgctc
     2881 cacaaaaaag gaggagcccg cgctgccgtg gtactgcccg ataatgtatt gtttgaagat
     2941 ggcgacgggg ctaaaatccg ccgcgacctc atggacaaat gcaacctgca caccatactc
     3001 cgcttgccta ccgggatatt ctatgcacaa ggggtaaaaa ccaacgtgct cttctttgaa
     3061 cgggcaacca cggagaccgg taacacgcag cagatttggt tctacgatat gcgtaccaac
     3121 atgcccacgt atggcaaacg cactcccttt aaccgctcgg ctttcgatgg tttcattaaa
     3181 gcctacaccg gaggtattac caccgataag gtacaattgg aatacgatgg caccattgac
     3241 cagactaagc gggctgccat taccgatgaa cggtggcagt gcattacccg tgagcagatt
     3301 gccgcgaaga acgactcgct cgatttgggc ttaattgccg acagctcgct caacaacggc
     3361 gacgctttag ccgacccgat tgacattgcc aaagaaacag ccaacgaatt gaaaagtatt
     3421 ttgggtgaat tggagatgat tattcaagaa ctaaaatgaa catgtaccga tgaagtccga
     3481 tgaaattaaa aacctattct tccaatttga agctgctgca tcagaattag aaggtgttga
     3541 atgctggagt gcacgtgaga ttcaaaattt gttagggtat agcaaatggg agaactttga
     3601 aaaagtaatt cagaaagcga aagaggcttg caaaactgcc ggcgaagaaa ttgactatca
     3661 ttttcctgac gtcaggaaga tgatcgcctt ggggaaaggt gctgaaaaag aaatagatga
     3721 catcctcctg acccgttatg cctgttattt ggttgcccaa aacggcgata gccgcaagga
     3781 ggaaattgcc tttgcccaaa actattttgc agttcaaacc cgtagggccg aattggttga
     3841 acaacgtatt ctggagtttg aacgggtcaa agcccgcgag aaattaagcc aaacagaaaa
     3901 acaactttcg ggcatacttt atgaacgtgg ggtcgacagc cagggatttg ctgtaataag
     3961 aagtaaaggc gaccaagctt tatttcggtt aaatactcaa atgctaaaac gtaagatggg
     4021 catacccgaa agccggccag ttgccgattt tcttccaaca atcagtatta aggcgaaaga
     4081 cctggcggcc gaaatgaccg ggttgaatgt gcaaagcaaa gatttaaaag ggcaaactaa
     4141 aattgagaaa gaacatgtcg acaacaacct tgctgttagg aatatgctca cacaacgagg
     4201 gataataccc gaaaatcttc ctcctggcga agatgtaaag aagcttcaac gaaagctcga
     4261 tggagatgag aaaaaactat tgaaaggaac caaaaagaaa aaataacctc aaacctcgaa
     4321 aggggcttag gacagtaata atttaaaaca actctacctt cattggtagg aaaagtcccc
     4381 ggaatttatc ccgcagaagg cgggagggga tttagggtag aaatttaggg gtaaataaga
     4441 tggagaatat attaccaaaa ggttgggtag cagttcaact aatcgaacta cttaaagttc
     4501 ttgaaaatgg ttcaaggcct attggaggtg tccaaggtat tttagaaggg ataccaagtt
     4561 tagggggaga acatttaaac agcgatggtg gcttcaaatt tgaaacaatt aaatatgttc
     4621 caatcgattt tgcagaaaaa atgcaaagag ga
//
LOCUS       DAOPUW010000060         4642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_18634_length_4642_cov_2.874646, whole genome shotgun sequence.
ACCESSION   DAOPUW010000060 DAOPUW010000000
VERSION     DAOPUW010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18634_length_4642_cov_2.874646"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PKJ43_01735"
     CDS             <1..462
                     /locus_tag="PKJ43_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNZ71309.1"
                     /translation="FRILKLSRYTSAGRTILNALWNSREKITVFIIFVIILAVIIGTF
                     MFLIEGPESGFKDIPTSIYWAIVTLTTVGYGDLSPTTHFGQMLSSFVMILGYSILAVP
                     TGIVTANIIGKQHRNTQVCSNCMYDKHDDDAHFCKRCGTSLEVGKEHNPIE"
     gene            618..1613
                     /locus_tag="PKJ43_01740"
     CDS             618..1613
                     /locus_tag="PKJ43_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ71310.1"
                     /translation="MTINQTILITGGAGFIGSNLCDAFIDKGFHVVCLDNFSTGKREN
                     IQHLIDNPRFSLIEGDIREPNDCAKAAHGADFVLHQAALGSVPRSINDPATSNNVNVG
                     GFVNMLVAARDAGVKRFVYAASSSTYGDSKNLPKVEDHIGKPLSPYAVTKYVNELYAE
                     VFARTYGISCVGLRYFNVFGKRQDPNGAYSAVIPLWVKQLINLQPPIINGDGSFSRDF
                     TYIDNVVEANYKALMASEENLKTPEGFSNQVFNIAYGNNTTLVQLFETLKLNLAKYEP
                     RIGQIEPIFGAQRPGDIPHSLASIEKAKRLIGYDPQFSAQQGFALACEWYWDNLK"
     gene            1656..2966
                     /locus_tag="PKJ43_01745"
     CDS             1656..2966
                     /locus_tag="PKJ43_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNZ71311.1"
                     /translation="MNNTFQPSIAVIGLGYVGLPLARLFATKYPVTGFDINKNRVAEL
                     MQGNDSTLEVDNITLKSVLQQSSSFKKDNKATGLFCTTNLEEIADCNIYIITVPTPVD
                     RNNRPDLTPLLRASETVGKVIKKGDIVIYESTVYPGATEEDCIPVIEKTSGLKFNLDF
                     FAGYSPERINPGDKEHTVEKIKKVTSGSTPEIAEVINNLYASVILAGTHLAPSIKVAE
                     AAKVIENSQRDINIAFVNELAKIFNWMGIDTHDVLEAAGTKWNFLPFKPGLVGGHCIG
                     VDPYYLAQKAQEAGYHPEIILAGRRLNDSMGQYVAAEVVKLMIKNNSPVRGARVLMLG
                     ITFKENCPDIRNSHAIDIYHELTSYGIEVEVYDPWAKPSEVQHEYGITSLTECPKSQN
                     YEAIILAVAHAKFQSIDLKAHKATGTVIYDVKGILDKQLVDGRL"
     gene            complement(3072..3920)
                     /locus_tag="PKJ43_01750"
     CDS             complement(3072..3920)
                     /locus_tag="PKJ43_01750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ71312.1"
                     /translation="MIKQLYRFLHPKFQNLFLEYKVSFKPRYGHGLPPHGELYRVIDA
                     KREEYKGLLNKALSLKESIWTIKDSSLETDSSKPAWNNGYLPGLDIVGIYTLLTEFKP
                     RKYIEIGSGNSTKVAYKAKIEQNLDTEIISIDPMPRAEIDNLADKIIRKPFENVDFDI
                     VNELNENDILFVDNSHRILPNSDAMVFFLEILPRLKKGVIVHIHDIYLPYDYPQDMCD
                     RFYSEQYGLAMYLLANTQKYEPLMPNYFIFEDKELSALIAPIWNHKNLQNVEKHGGSF
                     WLKIAE"
     gene            complement(4036..>4642)
                     /locus_tag="PKJ43_01755"
     CDS             complement(4036..>4642)
                     /locus_tag="PKJ43_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71313.1"
                     /translation="IHSYSSNESKSSLRWVPIYLIFMLGTFPLPFLAQFIARDALMSS
                     GFAFIQVALLITQHSFLAYHVVKGNYILQETTPEVLMPQANYSEKDNKNSSSLKKNKL
                     TRKQFEKYIQTTRPYLKADLKITDLVSDLQINRTYISSFINSEYGMNFSNYINTLRFN
                     EFNRLREHPSTKDKSTAELSEMAGFGSYRSYSRVLEMMKKA"
BASE COUNT         1418 a          990 c          944 g         1290 t
ORIGIN      
        1 ttccgcatac tcaaacttag ccgatacacc agtgcaggaa ggactatttt aaacgcgctc
       61 tggaacagca gggaaaaaat aacagtattc atcatatttg tgatcatact cgccgtaatc
      121 atcggaacat tcatgttcct gattgaaggc cccgaaagcg gatttaaaga catcccaacc
      181 agtatttatt gggccattgt tacccttacc acggttggct atggagattt atcaccaacc
      241 acacacttcg ggcaaatgct ttcgagtttt gtaatgatcc tgggctattc aattttggcc
      301 gttcctaccg gaattgtaac tgcaaatatt attgggaagc aacatcgcaa tacccaggtt
      361 tgctcgaatt gcatgtacga caagcacgac gacgatgctc atttctgcaa gcgttgcggg
      421 acatcactcg aggtgggaaa agaacataac ccgatagaat aatttcaacg atgattttaa
      481 ccgaaatgcc ttcaatatat cttttataaa ctaaatgctg ccaaaaacaa ttttgcgatg
      541 atgctttctg aagggcattt tcatgacaaa tcgaaaaaaa aatccattac atttgcagcc
      601 aaatactcag taattacatg accatcaatc aaactattct gattaccgga ggagcaggtt
      661 ttattggatc aaacctctgt gatgcattta tcgacaaggg ttttcacgtg gtatgcctcg
      721 acaatttctc gaccggaaag cgcgaaaata ttcaacacct catcgacaat ccaaggtttt
      781 cgcttatcga aggcgatatt cgcgagccaa acgattgtgc caaagctgcc catggagccg
      841 attttgtact ccatcaagcc gcgttgggaa gcgtgccaag atcaattaac gacccggcca
      901 caagcaacaa tgtcaacgtg ggaggctttg ttaatatgtt ggttgctgcc cgtgacgcgg
      961 gggtcaaacg cttcgtttat gcggcaagtt catcgaccta tggcgactct aaaaatttac
     1021 caaaagtgga agaccatatt gggaaacccc tttccccgta tgctgtaacc aaatatgtta
     1081 atgaacttta cgccgaagtt tttgcccgta cgtatggtat ttcttgtgta ggactccgct
     1141 atttcaatgt ttttgggaaa agacaagacc cgaatggtgc ctattctgct gttattccat
     1201 tgtgggtaaa acagttaatc aacctgcaac caccaattat aaatggcgac ggaagttttt
     1261 cacgcgattt cacttacatc gacaacgtgg ttgaagccaa ctacaaagcc ctaatggcat
     1321 cagaagaaaa cctgaaaacg cccgaaggat tttcaaatca ggtgtttaat atagcttatg
     1381 gtaacaatac taccctcgtt caacttttcg aaacgctgaa gttgaacttg gccaagtacg
     1441 agccacgtat tggccaaatc gaacctatct tcggagcgca acgcccgggc gatattcccc
     1501 actctctggc ttcaatcgag aaagcaaaaa ggcttatcgg atacgacccg caattcagcg
     1561 cacagcaagg gtttgccctg gcatgtgaat ggtattggga caaccttaag taattctcaa
     1621 ttaaccaaag agatgattta actaatttaa taatcatgaa caatacattt caacccagca
     1681 ttgcagtaat tggccttggt tatgtaggac tcccgctcgc ccggctattt gccacgaaat
     1741 accccgtaac aggcttcgat atcaacaaaa accgcgtagc tgaattaatg caaggtaacg
     1801 attcaacgct ggaggtagat aacataaccc tgaaatcggt acttcagcaa tcatcatcat
     1861 ttaagaaaga taataaagct accggcttat tttgcaccac aaatttagaa gagattgcag
     1921 actgcaacat atatatcatt accgttccca caccagttga cagaaataac cgccccgacc
     1981 ttaccccact gttacgtgct tcggaaaccg ttggaaaggt gataaaaaaa ggcgacatcg
     2041 ttatttatga aagcactgtt taccccggtg caaccgaaga agattgcatc ccggttattg
     2101 aaaagacatc cggtttaaaa ttcaatctcg atttttttgc agggtactca cccgaacgga
     2161 tcaaccctgg tgacaaagaa catacggtag agaagattaa aaaagtcacc tcaggatcga
     2221 cccccgaaat tgcagaagta atcaacaatc tgtacgcgtc ggttattttg gccggaaccc
     2281 atttagcgcc atcgataaaa gtggccgaag cagccaaagt aattgagaac tcacaacgcg
     2341 atataaacat tgcttttgta aatgagctgg caaaaatttt caactggatg gggatcgaca
     2401 cccatgatgt acttgaagca gccggaacaa aatggaactt tttgccgttt aaaccaggct
     2461 tggtcggagg acattgcatt ggagttgacc catactatct ggctcaaaag gcccaggaag
     2521 ctggctatca tcccgaaatt atccttgctg ggcgaagact caacgacagc atgggacagt
     2581 atgttgccgc agaggtagtt aagcttatga taaaaaacaa cagtccggtt cgtggagcac
     2641 gggttcttat gcttggaatt acgtttaaag aaaattgtcc cgacatcagg aactcgcatg
     2701 ccatcgatat ttaccacgaa ctgacaagct acggaattga agttgaggtg tacgacccgt
     2761 gggcaaaacc ctctgaagta caacacgaat atggcataac atcgctcaca gaatgcccca
     2821 aaagccaaaa ctatgaagcc ataatcctgg ctgttgcaca cgcaaagttc caatcaatcg
     2881 acctgaaggc tcacaaagcc acaggaacag tgatatacga tgttaaaggt attttagaca
     2941 aacaactagt cgacgggcgg ttgtaacgat ttcaaaaaac ctgttaagcg ggaattggac
     3001 tacaaataat gaagattact catctttcgg tgaaatccat ttcccgttca catttatagc
     3061 tttctattcc atcactcagc aatcttcaac caaaaagaac caccatgttt ttcaacattt
     3121 tgcaagttct tgtggttcca aattggtgca atcagtgccg atagttcttt atcttcaaag
     3181 atgaaataat taggcatcag gggttcgtat ttctgggtat tggccagcaa atacatggcg
     3241 agcccatatt gctcggaata aaacctgtcg cacatatcct gcggataatc atacggtaaa
     3301 tagatgtcgt gtatatgtac aattacccct tttttgagcc ttggcaaaat ctcgagaaag
     3361 aaaaccatgg catcagaatt gggtaatatg cgatgtgaat tatcaacaaa aaggatgtcg
     3421 ttttcattca attcattaac aatatcaaaa tcgacattct caaagggttt tctgatgatt
     3481 ttatcggcca gattatcaat ttcggccctt ggcatcggat cgattgaaat aatttctgta
     3541 tcaaggttct gttcaatctt ggctttatag gccacttttg ttgaatttcc cgaaccaatt
     3601 tcgatatatt tccgtggctt aaactctgtc aacaaggtat atataccaac aatgtcgagc
     3661 ccgggaaggt acccgttatt ccaggctggc tttgaagagt ctgtttcgag gctcgagtcc
     3721 tttatagtcc agatgctttc ttttaggctc aatgctttgt ttagcaagcc cttgtattcc
     3781 tccctctttg catcgattac cctatataat tctccatgag gaggtaatcc gtggccataa
     3841 cggggcttaa aactgacttt atattcgaga aaaagatttt gaaatttcgg atggagaaac
     3901 cggtatagtt gtttgatcat aaggttaaca aaataattta ttgtcaaaca aaagtaaaaa
     3961 agaattgcta agatgcacta tttgttttca ttttttgcct taaagcgaag cttcgaatgc
     4021 gaaaacagga ttacttcatg cttttttcat catttctaat acccgactgt aactgcgata
     4081 actgccaaaa ccggccattt cagaaagctc agctgtgctt ttatcttttg ttgatggatg
     4141 ttcgcgtagc cggttaaact cattaaaacg cagcgtatta atgtaattgc taaaattcat
     4201 tccatactcc gaattgataa atgacgaaat ataggtgcgg tttatttgaa ggtcggatac
     4261 caaatcggta atttttaggt cggctttcaa atacggtcgt gttgtttgta tatatttttc
     4321 aaattgctta cgtgttagtt tattcttttt gagtgaagag ctgttcttgt tatctttttc
     4381 gctgtagtta gcctgaggca ttaaaacttc aggagtagtt tcttgtaaaa tataattgcc
     4441 ttttacaaca tgataggcta aaaaactatg ttgtgtgata agcagcgcaa cctgtataaa
     4501 tgcgaagcct gaactcatta aggcatcgcg ggctataaat tgcgccagaa atggaagcgg
     4561 aaaggtgccc agcataaaaa ttaaataaat aggcacccag cgtagcgaac ttttcgactc
     4621 gtttgacgaa taactgtgga tg
//
LOCUS       DAOPUW010000061         4638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_18653_length_4638_cov_3.441196, whole genome shotgun sequence.
ACCESSION   DAOPUW010000061 DAOPUW010000000
VERSION     DAOPUW010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18653_length_4638_cov_3.441196"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PKJ43_01760"
     CDS             <1..467
                     /locus_tag="PKJ43_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71314.1"
                     /translation="DGKWLMFTLADHGYFSIFNKSSDLYLLNLETNDFFPYPFNSGDV
                     DSYHSWSGNSRWVAFSSKRLDGLCTRPYFSHVDEKGNFSKPFVMPQRDPLFYRSYKDN
                     YNVPELVNGKVNIDRASLLEAARDNALQANFDPNIDVDGLSGASRIEQSVLH"
     gene            421..1080
                     /locus_tag="PKJ43_01765"
     CDS             421..1080
                     /locus_tag="PKJ43_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNZ71315.1"
                     /translation="MAYRVPVGLNSLFYIEIVAMLSEIHEIIAKESAAILNIPVDDNF
                     PAAVDLIFKKVHSDGGKLVASGMGKAGQIAQNIATTFSSTGTPALFLHPSDAQHGDLG
                     VLARNDIMLLISNSGKTREIIELIELSRNLYEEIPIILITGNPKSEIARTAQVVISTG
                     GPEEVCPLGLTPSTSTTVMNVIGDVLVVLLMKKIGFTCADYAKRHHGGYLGLKSREQL
                     K"
     gene            1179..1721
                     /locus_tag="PKJ43_01770"
     CDS             1179..1721
                     /locus_tag="PKJ43_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNZ71316.1"
                     /translation="MRIIKEDCIGLVIDIQERLYPAMARKEEFLGNSCKLVLGLQELS
                     VPLIVTQQYSKGLGQTLVEVSSLISHFNPIEKVSFSCYDEPAFVEALEEADKKTVIIC
                     GIESHVCVLQTAVDLKAAGYTPVVIYDCTSSRNKDDWKIALERFRYENIMVLNHESLL
                     FELTRTAASSSFKSISKLVK"
     gene            2085..>4638
                     /locus_tag="PKJ43_01775"
     CDS             2085..>4638
                     /locus_tag="PKJ43_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNZ71317.1"
                     /translation="MEKFLHSVASYLYGKHHGHLEQLTVVFPNRRAGLFFQKYLSTLT
                     EKPIFSPSIITISEMVSGFSPLKVDDNSSLVVQLWETFMKITSIEEPLDDFFYWGEML
                     LSDFNDIDKYLVDAKQLFKNIISLKEIDSGFEYLSKEQILFLSTFWQHILQVKNSGEK
                     DSFIKNWANLFPVYNEFNKSLQTKGLAYEGMIYRQMAEMVKAGTSHWEGKNIAFVGFN
                     ALNNCEKALFDHVKINCKADFFWDFDQFYMQKNDHEAAFFMHSNLKRYPAPVDFSFSD
                     NNFEKLANIDLVSIPGFSGQAIYASNWLAENNQFINSLFDNTAIVLCDESLLPSVLSS
                     LPDTIADINITMGFPLKNSVVFALLKGLIDLDRNGRKGNNNETLYYYRNVLALLNNPL
                     IKPFVADFIESLNLRIKQNNLIYITKSEVVSDELLASVFEVPDDAPSVKEYLHAIIKK
                     LFARVPDDDPFVKEALYQLYLLVNRQHSTLFVNKSFSDRLVSKKLFYQLLLRSAEKMS
                     IPFEGEPLSGMQLMGFLETRCLDFENLILLSFNDEKLPGVQHQHSFIPNSLRKGFDLP
                     VIEHRNAMYAYYFYRLLQRAKRVTLVFDSRTEALSRGEVCRYAKQLKYEAADLTLVEK
                     QGVFNFETVENNPIIVEKDRGLLEKLEKIIAEKRLSPTMLNSYIECGLKFYFNYVESI
                     RESDDVSEEIDQLVFGKVAHSAMEELYKPFLGKTIEASFIKSMLDNHLLIDKVLFDAL
                     EKEFFKGGSFNKNGKNLLVIDIIRKYIRRMLKYDMCIAPLEIISLETEYTTNIDLKIN
                     DKLFRVTVGGIIDRLDRVGGRIRVVDYKSGRSLQKVQSVESLFIPHTNRNKAA"
BASE COUNT         1415 a          839 c          964 g         1420 t
ORIGIN      
        1 ccgacggaaa atggctgatg tttaccctag ctgaccatgg ttatttttca attttcaata
       61 agagcagcga tttgtacctg ctaaacctcg aaaccaatga cttctttccc tatccgttca
      121 atagcggcga tgtcgatagt tatcacagct ggtcggggaa cagtcgttgg gttgccttta
      181 gcagcaaacg tctcgacgga ttgtgtacca ggccttattt ttcacacgtc gatgaaaagg
      241 gcaactttag taaacctttt gtaatgccgc aacgcgaccc tttgttttat cgttcgtaca
      301 aagataatta taatgtaccc gagctggtga atggaaaagt aaacatcgac cgggcatcat
      361 tgctcgaagc tgcccgtgac aacgctttgc aggccaattt tgacccgaat attgatgttg
      421 atggcttatc gggtgccagt aggattgaac agtctgtttt acattgaaat tgtagctatg
      481 ctttcagaaa tacacgaaat aattgccaaa gagtcagccg cgatcctgaa tattcctgtc
      541 gatgacaatt ttccagcagc agttgacctt atcttcaaaa aagtgcattc agatggaggc
      601 aagcttgttg caagcggaat gggaaaagcg ggacagattg cccaaaatat tgcaacaacg
      661 tttagttcga caggtacacc tgcacttttt cttcacccaa gcgatgccca acatggagac
      721 cttggcgtat tggcacgaaa tgatataatg ctgcttattt cgaactcggg gaaaacccgt
      781 gagattattg agttgattga gctttctcga aacttatacg aagaaatccc tattatttta
      841 attacgggta atcctaaaag cgaaattgct cgtacggctc aggttgttat atctactggc
      901 gggccagaag aagtatgccc gctggggctt accccaagca catcaactac agtgatgaat
      961 gttattggcg atgtgttggt tgttttattg atgaagaaaa ttggctttac ttgtgccgac
     1021 tacgcaaaaa gacatcatgg cggatacctc ggcctgaaat cgcgagagca attgaaatag
     1081 aacattatct tttctttaaa cagtttttta cttcgaaatc gcccttaccg cattcggtaa
     1141 ataaaaccca tatttgtcca tgtaaaaaac aattagcgat gaggataatt aaagaagatt
     1201 gtattggttt ggtaatcgat attcaggagc ggttgtaccc tgctatggcc cgaaaagaag
     1261 agtttttggg caattcatgc aaattagtgc tgggtcttca ggagcttagc gtaccgctta
     1321 tcgtaactca gcaatattca aagggattag gacaaacatt ggtggaggtt tcttcgttaa
     1381 tcagtcattt taatccaatt gaaaaagttt cattcagttg ttatgatgaa ccagcctttg
     1441 ttgaagcact ggaagaggcc gataaaaaga ctgtaattat ctgcggaatc gaatcacatg
     1501 tttgtgtact tcaaacagct gtcgatttaa aagctgcagg ttatacccct gtggttattt
     1561 acgattgcac ctcgtcgaga aataaagatg actggaagat tgccctcgag aggttcagat
     1621 acgagaatat catggtacta aatcacgaat cgctactttt tgaacttaca agaacagcgg
     1681 caagcagctc gtttaagtca atctcgaagt tagttaagta ataaaaaaca aagcgggtta
     1741 tctgttttga taacccggct gttcatagtt tagttagatc agaaaataga tgatggttta
     1801 aagagaaaag gtagattata tttttaggta actctaacct gtttcgtttc taattctggg
     1861 tagccaatat ataaattttt tttaaaatca taaaaaacgt taacgaatat tttcctcaaa
     1921 accccattgc aaacgttttc gaacgtgcca acatcatggt tatttgcatg aacaatgtta
     1981 aattcattaa aatttgtttt tggaacttta ttgcaatttg ttttaaaatg ctgggacatc
     2041 aatactgatt ctttttctta tttttaccca tcaaaccaaa gaacatggaa aaatttctgc
     2101 atagtgtcgc ttcttatctc tatggtaagc atcatgggca tcttgaacag cttactgtcg
     2161 tttttcccaa cagacgtgcg ggattattct ttcaaaaata cttaagtact ttaacagaga
     2221 aacctatttt ctctccaagc ataattacca tttcagaaat ggtttcggga ttttcacctc
     2281 ttaaagtcga tgataacagc tcgttagttg tgcaattatg ggaaaccttt atgaaaatta
     2341 cgtcaataga ggagcccctc gacgattttt tttattgggg agaaatgctt ttgtccgact
     2401 ttaatgatat tgacaaatat cttgtcgatg caaaacagct tttcaaaaac ataatatcgc
     2461 tcaaagagat cgattcagga tttgaatacc tcagcaaaga acaaatcttg tttttgtcga
     2521 cattttggca acacattctg caagtaaaga attctggcga aaaagacagt tttataaaga
     2581 attgggctaa ccttttcccg gtatataatg aatttaacaa gagcctgcaa acaaaaggac
     2641 ttgcctatga ggggatgata tacaggcaaa tggcagaaat ggtaaaagca ggaacatcgc
     2701 attgggaagg gaaaaatatt gcatttgtag gctttaatgc tctaaataat tgcgagaaag
     2761 cactctttga tcatgtaaaa ataaattgta aggcagattt cttttgggat ttcgatcaat
     2821 tttatatgca gaaaaatgac catgaagctg ctttttttat gcattcaaac ctgaaaagat
     2881 atcctgcgcc tgttgacttt tcgttttcgg ataacaattt tgagaaatta gcaaatatcg
     2941 atttggtttc aattccagga ttctcggggc aagctattta tgcatcgaat tggctcgccg
     3001 agaataatca gttcattaat agtttgttcg acaatactgc gattgtgctt tgcgatgaat
     3061 ctctgttgcc ttcggtgctt tcttcgttac ccgatacgat tgccgacata aacattacca
     3121 tgggttttcc gctgaaaaac tcagttgttt ttgccctttt aaaaggcttg attgatttag
     3181 acagaaatgg gaggaaaggg aataacaacg agactttata ttattacagg aatgtgcttg
     3241 ccttgctaaa taacccatta atcaaacctt ttgttgcaga ctttattgaa agccttaacc
     3301 tcaggattaa gcagaacaac ctcatttaca tcacaaaaag tgaagtggta agcgatgaat
     3361 tactggcctc ggttttcgag gtacccgatg atgcgccttc ggtgaaagaa tacttacatg
     3421 ctatcatcaa aaagcttttt gctcgtgtac ctgacgatga tccatttgta aaagaagcac
     3481 tttaccagtt atatctgtta gttaatcgcc agcacagcac cttgttcgtt aataaatcat
     3541 tttcagacag gttagtctcg aaaaagctat tttaccagct tttgctccgt tctgccgaaa
     3601 aaatgagtat tccttttgaa ggagagcctc tttcaggaat gcaattgatg ggttttctcg
     3661 aaacacgttg cctcgacttt gaaaacctga ttctactgtc tttcaacgat gaaaaacttc
     3721 caggcgtaca acatcagcat tcgttcattc ccaattcatt gagaaaaggt ttcgatttgc
     3781 cagtaattga gcaccgtaat gcgatgtatg catattattt ctatcggttg ttgcagcggg
     3841 caaaacgggt tactttagtt ttcgattctc gtacagaagc cctttcgagg ggtgaagtgt
     3901 gccgctatgc aaaacaattg aaatatgagg cagccgacct cacgttggtc gaaaaacaag
     3961 gagttttcaa ttttgaaacc gttgaaaata acccgattat tgttgagaaa gatcgggggt
     4021 tgttggagaa acttgaaaaa ataattgccg aaaagcgcct tagtccaact atgctgaact
     4081 catatatcga gtgtggattg aagttttatt tcaattatgt agaatcgata cgcgaaagtg
     4141 atgatgtctc ggaagagatt gatcagttgg tatttggaaa ggttgcgcac tcggcaatgg
     4201 aagaattgta taaacctttt ctgggaaaaa caattgaggc atcttttatt aagtcaatgc
     4261 tcgacaatca tttactcatc gataaagttc ttttcgacgc gttggaaaag gaatttttta
     4321 aggggggaag tttcaataag aacgggaaaa atctcttggt aatcgacata atcagaaagt
     4381 atattcggcg aatgctgaaa tatgatatgt gcattgctcc tttggaaatt attagtttgg
     4441 aaactgaata tactaccaat attgatttga aaatcaatga taaattattc cgggttacgg
     4501 taggggggat aattgaccgg ctcgataggg ttggagggcg aattcgagtg gtcgattata
     4561 agtcgggccg ttcattacaa aaggttcaat cggttgaatc actatttata ccccatacaa
     4621 acaggaacaa agctgctt
//
LOCUS       DAOPUW010000062         4626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_18713_length_4626_cov_3.352221, whole genome shotgun sequence.
ACCESSION   DAOPUW010000062 DAOPUW010000000
VERSION     DAOPUW010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18713_length_4626_cov_3.352221"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="PKJ43_01780"
     CDS             <1..1252
                     /locus_tag="PKJ43_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ71318.1"
                     /translation="GKTTLLRIIAGFQKAGEGKLTIGGVDMTETPPHRRPVNTVFQRY
                     ALFPHLNVSNNIAFGLNLKKLDKEHIRKKVKKVLKMVSMTDYEERDVDSLSGGQQQRV
                     AIARAIVNEPEVLLLDEPLAALDLKMRKDMQLEIKAMHKKLGITFVYVTHDQEEALTL
                     SDTIVVMSEGKIQQIGTPTEIYNEPANSFVADFIGESNILNGKMIEDRLVEFAGHRFE
                     CVDKGFSPNEPVDVVVRPEDIYIFELSDAAQFTAKVTSSIFKGVHYEMMVTTPEGFEF
                     MIQDYHCFEVGESVGLRVKPSDIHVMKKERIHNTFEATITDKNHVRFLGQVFECKDCQ
                     HFEPAAEVHAHIEFNKVILQDNEEDGMISGDVSFILYKGDHYHLTVLTDEGENIFVDT
                     NDIWDDGDRVGITINPNAIKITTR"
     gene            1264..2064
                     /locus_tag="PKJ43_01785"
     CDS             1264..2064
                     /locus_tag="PKJ43_01785"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ71319.1"
                     /translation="MKKSFLLFAGKRKNWSIPYIIFLGFFVVIPLLLIVFYAFSSRTG
                     GFTLANFQKFWNQPEAMHTFIFSIGVAIITTVICLVLGYPAAYILSRMEAKTAKIMVL
                     LFILPMWVNFLIRTLATVALFDFLRIPLGQGSLYFGMVYNFLPFMVYPIYNSLQKTDK
                     SLIEAAQDLGATPRQVFLKTVLPLSVPGIASGIMMVFMPVISTFAIAELLTINNIKLF
                     GTTLQENIYNGMWNYGAALSLIMLILIGLTSLLNAEGNAKAAEEGGLL"
     gene            2061..2861
                     /locus_tag="PKJ43_01790"
     CDS             2061..2861
                     /locus_tag="PKJ43_01790"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ71320.1"
                     /translation="MKKILANSYIGSLLIMLYAPILIIIIFSFTEAKVLGNWTGFSLK
                     LYTNLFQSGVHHSLSSALWNTLIIGLISAFVSMLLGTVAAIGIYNLKPRARKIVQFAN
                     NIPILNPDIITAISLFLLFVSLGISQGYTTVILAHISFCTPYVVLSVYPRLRMMNENL
                     YEAALDLGATPFYALRKVLLPELRPGMISGFILAFTLSIDDFVVTLFTIGNEGLETLS
                     TYIYADARKGGLTPELRPLSAIIFITVLALLLVINHRAAKREQENQKH"
     gene            2895..4235
                     /locus_tag="PKJ43_01795"
     CDS             2895..4235
                     /locus_tag="PKJ43_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ71321.1"
                     /translation="MRKNNLIRLIQSTFVFAALIAVNCFSTQVQAQERNEVLKIYNWA
                     DYIDEDLLTEFPVWYKEQTGENVKIVYQVFDMNEVMYTKIAMGQEDFDLACPTQAVMQ
                     RMLHNDLLLPIDQNFGNTPNYMNNMSPFIIDRLDDFNAKGKNAAEYAIPYMWGVSGIL
                     YNTEKVNKSEMQSWGCFWDQKNKGKMLMKDSYWDAYATAAVWGYRNEIAAGKRSVYSV
                     TNDHSQADINLVEKELKTLKPNLAGWEADFGKEMMTKGEIWFCYAWNGDAVWAIEEAA
                     EVGVNLDFAVSSEDSNVLFDCWVIPKYAKNTKAANYFLNYICRPDIALRNMDVSGYCS
                     AVASTEILEAVTDSTLEETYNLSYFFGPGNDSLRVNPVQYPDKSVVDRCAIINDYLDK
                     NDEVLEMWSRAKGDNLFGGMTFMIIAVIVLLAGWIILRQAQKAKSKKRRSGRRK"
     gene            complement(4295..>4626)
                     /locus_tag="PKJ43_01800"
     CDS             complement(4295..>4626)
                     /locus_tag="PKJ43_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HNZ71322.1"
                     /translation="AFGCSSSDIIAAIGPCIGKMNYEVGREVVEAMSLVLDNPSESIT
                     ARHGKNEKFCLDLVQANVQLLCQAGVNLDNIDYFSFCTFSEPELFYSARRDGSKTGRM
                     LSGIMIQ"
BASE COUNT         1402 a          992 c          966 g         1266 t
ORIGIN      
        1 cggaaaaaca accctgttgc gcattattgc cggttttcaa aaagctggtg aaggaaaatt
       61 aaccatcggc ggagtagaca tgaccgaaac ccctcctcac agacgcccgg taaatacggt
      121 ctttcaacgc tatgccttgt ttccacacct caacgtttca aataatattg cctttggcct
      181 caatctgaag aagctagata aggaacatat caggaaaaag gtgaaaaaag tacttaagat
      241 ggtgagcatg accgactatg aagaacgcga tgttgattca ttgtcgggag ggcaacaaca
      301 gcgtgtggcc attgcccgcg ccattgttaa cgaaccggaa gtgcttttgc ttgacgaacc
      361 tctggccgcg ctcgacctga aaatgagaaa agacatgcag ctcgagataa aagccatgca
      421 caaaaaactg ggaatcacct ttgtatatgt tacccacgac caggaagagg cccttacgct
      481 cagtgatacc attgtggtta tgagcgaagg aaaaattcaa cagataggaa caccaactga
      541 aatatataac gaacctgcca attcgtttgt tgccgacttt ataggagaaa gtaacatatt
      601 gaacggcaaa atgatagaag atcgattggt cgaatttgct ggccatcgtt tcgaatgtgt
      661 cgataaaggc ttcagcccta acgaaccagt agatgtagta gtgagacccg aggatatata
      721 catttttgaa ctttctgatg cagcacaatt tacggctaaa gttacatcga gtatcttcaa
      781 aggagttcat tacgaaatga tggtaaccac gcctgaaggc ttcgagttta tgattcagga
      841 ttaccattgc ttcgaagttg gcgaaagcgt tggcctcagg gtaaagcctt ccgatattca
      901 tgtcatgaaa aaagaacgga tacacaatac tttcgaagcc acaataaccg acaaaaacca
      961 cgtacgtttc cttgggcaag tttttgaatg taaagattgt cagcactttg agcctgcagc
     1021 cgaagtgcat gcacatattg aattcaacaa agttatcctt caggacaatg aagaagatgg
     1081 aatgatcagc ggcgatgtaa gttttatact ctataaaggc gatcattacc acttgactgt
     1141 gttaacagat gaaggtgaga acatttttgt cgacacgaat gatatttggg atgacggtga
     1201 ccgtgtaggg ataaccatca accccaatgc aataaaaata acaacgcgct aattcaggga
     1261 aaggtgaaaa agagcttctt attatttgcg ggcaaaagga aaaactggtc gattccctat
     1321 attatttttc ttggcttttt tgtagtaata ccgctcttac tcattgtttt ctatgcattt
     1381 tcttcacgta caggaggttt taccttggcc aatttccaaa agttctggaa tcagcccgaa
     1441 gcaatgcata cattcatctt ttctattggt gtagccataa ttaccaccgt gatttgcctt
     1501 gttttgggtt atccggcagc atatatatta agccgcatgg aagccaaaac agccaaaata
     1561 atggtactcc ttttcattct gcccatgtgg gtcaattttc ttattcgtac cctggcaacc
     1621 gttgccctgt tcgatttttt aagaatcccg ctaggacaag gatcactcta ttttgggatg
     1681 gtgtacaact ttctgccgtt catggtttac ccaatttaca attcacttca aaaaaccgat
     1741 aagagcctta ttgaagcagc tcaggatctt ggagccacac cgcggcaagt cttcctaaaa
     1801 acagttttgc cgctcagtgt tcctggcatt gccagcggaa ttatgatggt ttttatgccg
     1861 gtaatatcta cttttgccat tgcagaactt ctcacaatca acaatataaa actttttggt
     1921 actacattgc aggaaaatat ttacaacgga atgtggaact acggtgcggc actttcactc
     1981 atcatgctta ttctcattgg cttaacctca ttgcttaatg ccgaaggaaa tgccaaagca
     2041 gccgaggagg gaggtctgtt atgaagaaga ttctcgccaa ttcctatatc ggttctttgc
     2101 ttatcatgct gtatgccccg atactgatta tcatcatttt ctcgtttacc gaagcaaaag
     2161 tacttggcaa ctggaccggt ttttcgctga agctgtacac aaacctgttt caaagcgggg
     2221 tacaccattc gctttcgagc gccctttgga acactttaat catagggtta atttctgcct
     2281 ttgtatcgat gctgcttggt actgttgctg ccataggaat atataatcta aaacccaggg
     2341 ctcgcaaaat tgtgcagttt gccaacaata ttcctatttt gaaccccgac atcattactg
     2401 ccatctcgct gttccttctt tttgtaagct taggtatttc acaggggtat accacagtaa
     2461 ttttagctca tatctctttt tgtactccat acgtggtttt aagcgtttac cctcgtttac
     2521 gaatgatgaa cgaaaacttg tacgaggcag ccctcgattt gggtgcaaca cccttctatg
     2581 ccctgcgcaa agtactcctg cccgaattgc ggccaggaat gatcagtggt tttatcctgg
     2641 cttttaccct ctcgatcgac gactttgtag ttactctttt caccattgga aacgaaggtc
     2701 tcgaaacact atcgacctac atttatgccg acgcacgtaa aggggggtta actcctgaac
     2761 taaggccact ttcggccatt atattcatta ctgttttggc tctgcttctg gtaataaatc
     2821 atcgcgcagc aaaacgcgaa caggaaaacc aaaaacacta attctaaaaa ggaatcaata
     2881 aaatgaaaga taaaatgaga aaaaacaacc tgattcggct aatacaatcc acatttgtat
     2941 tcgctgctct catcgcagtt aactgttttt caacccaagt tcaggctcaa gaacggaacg
     3001 aagtactcaa aatatacaat tgggccgatt acatcgatga ggatttactg accgaattcc
     3061 cagtctggta taaagagcaa actggtgaaa atgtgaaaat tgtatatcag gttttcgaca
     3121 tgaatgaagt gatgtacacc aaaattgcta tgggacagga agatttcgac cttgcatgcc
     3181 cgacacaagc agtgatgcaa cggatgcttc ataacgactt gttattgcca atagatcaga
     3241 attttgggaa tactccaaat tatatgaaca atatgtcgcc tttcatcatt gacaggctcg
     3301 acgatttcaa cgccaaagga aaaaatgctg ccgaatatgc cattccatac atgtggggtg
     3361 tatcgggaat attatacaac accgaaaaag ttaataaaag cgagatgcaa tcgtggggct
     3421 gcttctggga tcagaaaaac aagggaaaga tgctgatgaa agacagttac tgggatgcat
     3481 acgccacagc cgctgtgtgg ggttatcgta atgaaatcgc cgcagggaaa cgctcggttt
     3541 acagtgtcac caatgatcac tcgcaggccg atatcaacct cgttgagaaa gaattaaaaa
     3601 ccttaaaacc aaaccttgcc ggatgggaag ccgactttgg aaaagaaatg atgaccaaag
     3661 gcgaaatatg gttctgctat gcatggaatg gtgatgctgt ttgggccatt gaagaagctg
     3721 ccgaagttgg tgtaaatctt gactttgcag tatcctcaga agacagtaat gtattgttcg
     3781 actgttgggt aattcccaaa tatgctaaaa acacaaaagc tgctaattat ttcctcaatt
     3841 acatttgccg ccccgatata gccctccgta acatggatgt gagcgggtat tgcagtgccg
     3901 tagccagtac cgaaattttg gaggcagtta ccgattcaac ccttgaagaa acttataatc
     3961 tcagttattt ctttggcccc ggaaacgata gcttgcgagt gaacccagtt caatatcccg
     4021 acaaaagcgt ggtcgatcgt tgcgcaatta taaacgatta tctcgataaa aacgacgaag
     4081 tgcttgaaat gtggagccgc gccaaaggcg ataatctttt tggaggaatg acctttatga
     4141 ttattgccgt aattgttttg cttgccggct ggattatact tcgacaagcg caaaaagcaa
     4201 aatcgaaaaa gcgtagatcg ggcagaagaa aataatttcg aattcaactg aatatgaaac
     4261 ccacagctga agattttgcc taaagttgtg ggttttattg tatcataatt ccgctcagca
     4321 ttcgccctgt cttgctacca tctctcctgg ccgagtaaaa aagttctggt tcagaaaagg
     4381 tacagaaaga aaaataatcg atgttatcca gattaacacc cgcctgacat aaaagctgaa
     4441 cattagcctg aacaagatct aaacaaaatt tttcattttt accatgccgg gcagttatcg
     4501 attcagacgg attgtccaac accaaagaca tcgcctcaac aacttcacgc ccaacttcgt
     4561 aattcatctt tccaatacaa ggcccaatgg ccgcaataat atctgaagac gaacagccaa
     4621 aagctt
//
LOCUS       DAOPUW010000063         4603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_18828_length_4603_cov_3.176121, whole genome shotgun sequence.
ACCESSION   DAOPUW010000063 DAOPUW010000000
VERSION     DAOPUW010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18828_length_4603_cov_3.176121"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PKJ43_01805"
     CDS             complement(<1..704)
                     /locus_tag="PKJ43_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNZ71323.1"
                     /translation="MIIALTGASGYIATYFREHSPFPDAVFLPLNRDESDQVWRDRIF
                     RADVIVNLAGYPVSKRWTSRNRKQILESRVHTTRRIVTILNSLPHSGHKKLFISASAT
                     GIYPDNSADIFTESDETFSHDFLGKVVRKWESEAMKLENPLVRLVIVRFGVVLGDNSP
                     IISRLIPVFKAGFGGTLGRGNQIMPFVHVEDILSAFRFFVNNSQAAGVYNLVVPQIIT
                     NKDFTIALAKRLKKPS"
     gene            837..1856
                     /locus_tag="PKJ43_01810"
     CDS             837..1856
                     /locus_tag="PKJ43_01810"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HNZ71324.1"
                     /translation="MNRYILVALIIISCITSTLAQTQKDTIYTLYTATPIVIDASDAD
                     ECWSKSEWHPMKQVWLGNAMAEGDFEGKFKVCWDKDYLYVLVEAIDNILSDDHANPTT
                     NYWDDDCVEIFVDEDRSGGNHQYNNSAFAYHCSVFYDVIDAAGPNGATINCKNNIIMK
                     MDTIAENTYRWEFAVKMFNKNFNYNNPDPSRVFLSPNKLMGFSIAYCDNDETTARENF
                     IGSVVISAEEKNVSYINCDYFGTMLLVDPDGKYVSSNQLPGTKKKFSVYPNPVNNMLV
                     IENQGANTEGFTLTILTTNGQVVSETKLGKNKQTIDLQGLSNGTYLVSLRGDNYIQTE
                     TISKK"
     gene            complement(1965..3329)
                     /gene="hisS"
                     /locus_tag="PKJ43_01815"
     CDS             complement(1965..3329)
                     /gene="hisS"
                     /locus_tag="PKJ43_01815"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNZ71325.1"
                     /translation="MAQNPSIPKGTRDFFPSQMIRRNYIFDTVKSVFQLYGFQPIETP
                     AMENLSTLMGKYGEEGDKLLFKVLNSGDFMSNVPDELLSSKDSTKTTSKISEKGLRYD
                     LTVPFARFVVQNRNELAFPFKRYQIQPVWRADRPQKGRYREFFQCDVDVIGSNSLLNE
                     VELIQIVNEVYRRLNISVTLKINNRKILSGIAETIGEADRIVDITVAIDKLDKIGLDK
                     VNQELEQKGVSKNAIEKLQPILVLSGSTEEKLSQIASVISGSEIGMKGVEEMNTIFSY
                     LKKFDLGIAVELDLTLARGLNYYTGAIFEVKANDVQIGSITGGGRYDDLTGIFGLPGV
                     SGVGISFGADRIYDVMEQLNLFPTEALAGAKVLFINFGKDEEAYVLPAVSRMRSVGIS
                     CEIYPEAAKMKKQMEYANRKQIPYVILAGETEISDGKLTLKNMLTGEQSLLSVDEIIS
                     LLAN"
     gene            3545..4162
                     /locus_tag="PKJ43_01820"
     CDS             3545..4162
                     /locus_tag="PKJ43_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNZ71326.1"
                     /translation="MDIRNKIIEGAGELFVENGIKLVTMDMIAQSIGISKRTIYENFR
                     DKNDLVNNFISLAMVDHKKRAYEILAGSKNVIEALFNFGIFNQSAMKDVNPLFFNDLK
                     RYHGEVFHKNILTGEARNFELTYTILRRGQNEGVFIKEINLEIANQFIHYMFDFFQKM
                     KEDKQCNQNLIWTSVILPYLRGICTEKGQDILVFFLKNTENQNNI"
     gene            4184..>4603
                     /locus_tag="PKJ43_01825"
     CDS             4184..>4603
                     /locus_tag="PKJ43_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNZ71327.1"
                     /translation="MKQQALILIFLLLTGFIARSQEPLRLSLSEAVDYAVEHNKTLQN
                     AKLDVKASELKVKETISQGLPQIDAGVDFTTYFGYEMAFKLGGGSGNYTEQQLTEAWQ
                     QTSAQFPAFTQADLMNYMAGSTYTGILSAMNPMAIKMT"
BASE COUNT         1491 a          929 c          861 g         1322 t
ORIGIN      
        1 attgaaggtt tctttagacg cttagccaag gctatggtaa aatctttatt agttattatt
       61 tgtggaacaa ccagattgta aactcccgcc gcctgactat tattgacaaa aaacctgaat
      121 gcgctcaaaa tatcctcgac atgaacgaaa ggcattattt gattcccccg gccaagagtg
      181 cctccaaaac cagccttaaa aacaggtata agccgactga ttatcggcga attatcacct
      241 aaaaccactc caaaccgaac aataaccaat cgaaccaaag gattttccag tttcatcgct
      301 tctgattccc atttacgaac caccttccct aaaaagtcat gactaaaagt ctcgtcagat
      361 tcagtaaaaa tatctgcaga attatcggga tatatacctg tagcagaagc tgaaataaac
      421 aatttcttat gtcctgaatg aggaagggaa tttagaatag ttacaattcg tcgggttgta
      481 tgcacccggc tctccaaaat ctgtttccgg tttctcgaag tccaacgttt cgaaacaggg
      541 taaccagcca aattcacaat aacatcagca cgaaaaatac gatctcgcca cacttgatct
      601 gactcatcac ggttcagcgg aaggaagact gcatcgggga acggggaatg ttcgcgaaaa
      661 taggtggcaa tgtagcccga agcaccagtt aatgctatta tcatatactc aaggaattaa
      721 taataaaaga ataacagaaa cacacgccat gttgttttta tgaatgatca caatttgcaa
      781 ttacaaaacg aaaatataac tttagcaact ttatcaatta aaccaaaagt aatattatga
      841 accgttacat tcttgttgca ttaattataa tctcctgtat aacaagcaca ttagcacaaa
      901 cacaaaaaga caccatatat actctttata cagctacccc aattgtcatc gatgcttctg
      961 atgcagacga gtgctggtcc aagtccgaat ggcacccaat gaaacaggtt tggttaggaa
     1021 atgccatggc cgaaggggac tttgaaggta agttcaaagt atgctgggat aaggattatt
     1081 tgtacgtatt ggttgaagct atcgataata tcctctccga cgatcatgca aacccaacca
     1141 caaattattg ggacgacgat tgtgttgaaa tatttgtaga tgaagatcgt tcgggcggaa
     1201 accatcagta taataacagc gcatttgcat atcattgcag tgttttctac gatgtaattg
     1261 acgctgccgg acccaatggt gcaaccatta attgcaagaa caacatcatt atgaagatgg
     1321 atactatcgc tgaaaacacc tatcgctggg aatttgcagt taaaatgttt aacaaaaatt
     1381 tcaactataa taacccagac ccaagtcggg ttttcctctc tcctaacaaa ttaatgggct
     1441 tttcgattgc ttattgcgac aatgatgaaa caacagcgcg tgaaaacttc attggttcgg
     1501 tagtgatttc cgcagaggaa aaaaacgtca gctacatcaa ttgcgactat tttggaacca
     1561 tgctgcttgt tgatccagat gggaaatatg tttcctcaaa tcagttaccc ggcacaaaaa
     1621 aaaagttcag cgtataccct aacccggtaa ataacatgct agtgattgag aaccaaggcg
     1681 caaataccga aggttttaca ttgactatac tcacaacaaa cggtcaggtt gtgagcgaaa
     1741 ccaagcttgg caaaaacaaa caaaccattg atttacaagg actatcaaat ggaacttacc
     1801 tcgtatcatt acgcggcgac aattatattc aaaccgaaac tatcagtaaa aaataggtaa
     1861 agagcccgtg ctggttgaat tttgcgaagg cttaattgaa cattacttac agatttgctc
     1921 tattagaaaa tgtattctga aaacagaaaa gtatactatt ttaattaatt agctaataac
     1981 gaaattattt catcaaccga aagcagtgat tgttcaccgg ttaacatgtt tttgagtgta
     2041 agttttccat cactaatctc tgtctcaccg gcaagaataa catacggtat ttgtttgcga
     2101 ttggcatatt ccatctgctt tttcattttt gcagcttcgg gatatatttc gcacgaaatg
     2161 ccaacgcttc tcattcgact cacagccggt aacacatatg cttcttcatc tttcccaaag
     2221 ttgataaaca acacttttgc tccagcaagt gcttctgtag ggaataggtt aagttgttcc
     2281 attacatcat aaatgcggtc ggcgccaaac gaaattccta cgccagaaac accaggtagc
     2341 ccaaaaattc cggtcaggtc atcgtaacgg cctccgcctg taatgctccc aatttgaaca
     2401 tcgttggctt ttacttcaaa aatagccccg gtgtaataat ttaaaccgcg ggcaagagta
     2461 agatccagtt caacagctat accgaggtcg aatttcttca ggtaagagaa gattgtattc
     2521 atctcttcaa cacctttcat gccaatttct gaaccagaaa taaccgatgc aatctggctc
     2581 agtttttctt ctgtcgagcc acttaacact aaaataggtt gtaacttttc gatagcattt
     2641 tttgatacgc ctttctgttc aagttcttgg tttactttat ctaaaccgat tttatctagt
     2701 ttgtcgatag caactgtaat gtcaacaatc cggtcggctt cgccaatggt ttctgcaatt
     2761 cctgaaagaa ttttgcggtt attgattttt agggtaacac taatgtttaa acggcgatat
     2821 acctcgttaa caatctgtat cagttcaact tcgttcaaaa gtgagttgct gccaattacg
     2881 tcaacatcgc actggaaaaa ttcgcggtaa cgtccttttt gtgggcggtc ggcacgccaa
     2941 actggttgaa tttggtaacg tttgaacgga aacgcaagtt cgttgcggtt ttgaaccaca
     3001 aaacgcgcaa atggaacggt aagatcgtag cgtaatcctt tttcacttat tttacttgtt
     3061 gttttcgttg aatctttact gctcagaagc tcatcgggta cgttcgacat gaaatcgccc
     3121 gagttgagga ctttgaatag caatttgtcg ccttcttcgc cgtattttcc catcaatgtc
     3181 gataggtttt ccattgcagg tgtttcaata ggctgaaaac cataaagttg aaaaactgat
     3241 ttgacagtat cgaaaatgta atttcttctg atcatttgcg aagggaaaaa gtcgcgggta
     3301 cctttaggta tcgaagggtt ttgtgccatt atattataga attaaatagg tgcgcaaaat
     3361 taatgaaaca aaacaactga tgcaaaactc gttcaacttc gaataaagtt tttggtaaca
     3421 ttctgttaat ataaggtctt caaaccttct cttttcgtcc atttaccgat tatttaccaa
     3481 aagttaagaa agaaaacttt tttaactcca atagttttct acatttgcgc tctgattttg
     3541 atctatggat ataagaaata aaattattga gggtgctggg gaactatttg ttgaaaatgg
     3601 gatcaaactg gtaacaatgg atatgattgc ccaatcgata ggaatttcga agcgtacgat
     3661 ttacgaaaac ttcagggaca agaatgattt ggtaaacaat ttcatctcac ttgctatggt
     3721 tgaccataaa aaaagagctt atgaaatttt agcggggtcg aagaatgtaa ttgaagcact
     3781 cttcaatttt ggcattttca accaaagtgc aatgaaagac gtaaacccac tttttttcaa
     3841 cgatttaaaa agatatcacg gagaagtatt tcataagaat atactaacag gagaagctcg
     3901 aaatttcgaa ttaacctata ctattttaag aagaggacaa aacgaagggg ttttcataaa
     3961 agaaatcaac cttgaaattg ccaaccagtt cattcactac atgttcgact tttttcaaaa
     4021 gatgaaagaa gacaagcagt gcaaccagaa cctgatatgg acttcggtga tattacctta
     4081 ccttagaggc atttgcactg aaaagggaca agatatttta gtatttttct taaaaaacac
     4141 agaaaaccag aataatattt aaagttttac aacaataaca gctatgaaac aacaagcatt
     4201 aatcttaata tttttactac tgaccggatt cattgcccga agtcaggagc cccttcgcct
     4261 atcgctaagc gaagcggtcg attatgccgt tgagcataac aagaccttgc aaaatgccaa
     4321 actcgacgta aaggcatccg aacttaaggt aaaggaaacc atttcgcaag gcttgccaca
     4381 aattgatgcg ggagttgact tcactaccta ttttggctat gaaatggctt tcaagttagg
     4441 tggtggttcg ggtaattaca ccgaacaaca gcttaccgaa gcttggcagc aaacctcagc
     4501 ccagttcccg gcttttaccc aagccgattt aatgaactac atggcaggaa gtacttacac
     4561 aggcattctt tcggccatga accctatggc aattaaaatg acc
//
LOCUS       DAOPUW010000064         4528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_19231_length_4528_cov_3.233848, whole genome shotgun sequence.
ACCESSION   DAOPUW010000064 DAOPUW010000000
VERSION     DAOPUW010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19231_length_4528_cov_3.233848"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(331..1455)
                     /locus_tag="PKJ43_01830"
     CDS             complement(331..1455)
                     /locus_tag="PKJ43_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71328.1"
                     /translation="MEIQIREVKTKQDLSSFVYLPEKIHKNHKNWAPPIYSDDMVFFD
                     PKKNKSFDECDYQILIAIKDGEVVGRCMGLVHHIYNKEHNEKCGRFSFLETYDDYEVF
                     KKLIDHIAQWAKAKGMEQLVGPLAFSDHDPQGMLYEGYDEINVIASNCNFPYLTDFCV
                     KYNFEKKVDLVEYKIDIPEQFPEIYNKIGERFRLNQKNIRIEEYKTKLQFRPIIKPVL
                     TLINKTFGNVYGFTPFTDKEMSDYANRYIFLLSPHFIKVAKNENDEVIGTVVGIPDFS
                     EGFKACKGRLWPFGFLKLLTSGRKTKMIVLLLGAVDQRYQGRGIDVYMGIKMLESAKK
                     QGYKYIDSHLELETNTKVRAEMERMGGVIFKKYRIYQKDL"
     gene            complement(1788..2465)
                     /locus_tag="PKJ43_01835"
     CDS             complement(1788..2465)
                     /locus_tag="PKJ43_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNZ71329.1"
                     /translation="MKTTKTFILFIILLLAKASFAQSAFEWPVKELDTAREVTYLSNE
                     EKDMILEMNKVRYNPTMYAQKEMKWMGQHFEGKILKIPGEENLLTSEGKNAYEECIKY
                     LETAKPAPLLYPSKGMTKACRLLVYDQEVTGATGHRGSGNSTPSERVKKFGTFMGNFA
                     ENLHYGNSSASFAMIYLLIDDGEKSRGHRLAILNPSFNNVGVAIGKHKTYSKMFVSNF
                     ATHFTEK"
     gene            complement(2856..4031)
                     /locus_tag="PKJ43_01840"
     CDS             complement(2856..4031)
                     /locus_tag="PKJ43_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNZ71330.1"
                     /translation="MKFDFSFSHLPDLHFGAGKLNHLPEIISHWGNNILLVTGENSFS
                     HSVYYEHLTSRLKTQCILWRELMIGHEPSPSDIDQGVQLYRDSLPDVVVAIGGGSVID
                     AGKALSAMLVSELPIVHYLEQVGDTQPTGKKVPFIAVPTTAGTGSEATKNAVISVVGN
                     GGFKSSLRHDNYIPNVAIIDPELQLTCPASLTACSGMDAFSQLVESYLSTNANPMTDT
                     LALDGIMHIVQSLEELVLCQPTNLLLRSKMAYAAWLSGITLANAGLGTVHGFASSVGA
                     TTEIPHGAVCGSLMAETNKITLQELKKSNEQGISLQKYATLGRIIGKYEGKPNYFLAE
                     YFIDYLEQLTEKLKLPKLGQFGVKGNDLESIASKTSNKNNPVKLSHEQLVKILENRI"
     gene            complement(4042..4335)
                     /locus_tag="PKJ43_01845"
     CDS             complement(4042..4335)
                     /locus_tag="PKJ43_01845"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNZ71331.1"
                     /translation="MAITTCVHVQVVPDKIDDFIKASLENHFHSVKEPGNLRFDICQN
                     SSNPCSFMLYEAYTDEQSAANHKETAHYLKWRDEVATMMAEPRKGVKYNLLAH"
BASE COUNT         1228 a          845 c          917 g         1538 t
ORIGIN      
        1 gtaagtttac ctatctcatt ttgggcaaaa aagataattt cgatcgtaag gcactacagt
       61 cgttgggcga agtgcgtgag ttaagtctgg aagaactttt tggatactaa tgtgatacgt
      121 attcaattgt aaggatttag tacttatatt taatgaataa gccccgcaga actacaactg
      181 cggggcttat accagatgca attctaaatc agcccgaacc tattcatata taatgccgaa
      241 gaatagaaat gttcagtaca atcgagtggg cgaaggttgg acttttacat ttcttcaatc
      301 ggtattattt tgtattgatg ttgaatgtga ttacagatct ttttgataga tccgatattt
      361 tttaaagata actccaccca ttctttccat ttcggcccga acttttgtgt tggtctcaag
      421 ttcaagatgt gagtctatgt atttgtatcc ttgtttcttt gccgattcaa gcattttaat
      481 gcccatgtat acgtcgatgc cgcgcccttg gtaacgttga tcaactgccc ccagcaataa
      541 tacaatcatt ttggtctttc gtccagaagt aagaagtttc agaaaaccga atggccataa
      601 acgtccttta caagccttaa aaccttcact aaaatcagga atacctacaa cagtgccaat
      661 tacttcatcg ttctcatttt tagctacttt gataaaatga gggctcaaaa ggaaaatata
      721 ccggttggca tagtcgctca tctctttgtc ggtaaaaggt gtgaagccgt acacattacc
      781 aaaagtcttg tttataaggg ttaacaccgg ttttattatt ggcctgaatt gaagttttgt
      841 tttgtattcc tcaatgcgaa tgtttttttg attaagcctg aaccgttcac caattttatt
      901 gtagatttcc ggaaattgtt ctgggatatc tattttgtat tcaacaagat cgactttctt
      961 ttcgaaattg tatttcacac agaaatctgt caggtacgga aagttacagt tcgacgcaat
     1021 tacgttaatc tcatcatatc cttcatataa catgccttga ggatcgtgat cagagaatgc
     1081 taacgggcca acgagttgtt ccatcccttt cgctttagcc cattgggcaa tatgatcgat
     1141 gagttttttg aaaacctcat aatcgtcata ggtttcaaga aaagaaaaac gtccgcattt
     1201 ttcgttgtgt tccttgttgt atatatggtg gaccaacccc atacagcgtc ctacaacttc
     1261 tccatcttta atggcaataa gaatttgata atcacactcg tcgaatgatt tattcttttt
     1321 cgggtcgaag aaaaccatgt cgtcagaata aattggaggt gcccaattct tgtggttttt
     1381 atgaattttc tcggggagat atacaaacga tgaaaggtct tgttttgtct ttacttctct
     1441 aatctgaatt tccatactgt cttaagtttg aaaaatcgaa gatatagaaa aactagctta
     1501 aaattcgctt aacaattaaa aaagatttat ttcatgtgca taaatttaca gtgttagtgt
     1561 ctgtaatttt cataatccgc tttctttctt ctacttaaat tttgtagaaa taggatttaa
     1621 tgtccgaaac cagtttcgct aataaccctt tgttaatttt tgcatggtga gagcttcggc
     1681 atataaagct atttctcgta ccctatgttt tattatttta cactaattgt gttttcaatc
     1741 atcaaataat taatgatggt gtttcggtta acaatgattc ttattcctta cttttcggtg
     1801 aaatgagtgg caaaattgct tacgaacatt ttactgtatg ttttgtgttt tccaatagct
     1861 acacccacgt tgttaaacga tgggttgagt atggcaagac ggtggccacg cgatttttcg
     1921 ccatcgtcga taagtaagta aatcatggca aatgaggcac tagaatttcc gtagtgcaag
     1981 ttttcggcaa aattgcccat aaaggttccg aattttttta cccgctccga aggggtggag
     2041 tttccactac cccgatgacc agtagcaccc gttacttcct gatcgtaaac taataaacgg
     2101 catgctttgg tcattccttt cgatgggtaa aggagtgggg ctggttttgc tgtttcaagg
     2161 tattttatgc attcttcgta agcatttttt ccttcagagg ttaacaggtt ctcttctccg
     2221 gggattttca gtatcttgcc ctcgaagtgt tgtcccatcc atttcatttc tttttgggcg
     2281 tacattgtcg gattatatcg cactttattc atttccagaa tcatatcttt ctcttcgtta
     2341 gatagataag tgacttctct agcggtgtcg agttctttta cgggccactc gaatgccgat
     2401 tgcgcaaaac ttgcttttgc cagaagtaaa atgatgaata aaataaatgt ttttgtcgtt
     2461 ttcattgtgc tatttgtttg tgtgttaatg tgtttgttgg agtttgtgtg tcctgaaacc
     2521 agagtggtca aaaacctatt gtaacccgtt tttcacttgg cagatcgaca gtgaaaagcc
     2581 tctgtatgtt gcgctcaaat tctgatcatc tggtttttta aaggtgataa aaaaaatgaa
     2641 cttttcagag ctcgggcatt ataatcttca aatattgtat catttttgat gtcttactgg
     2701 cttacttaaa cataaagtta acattccaat ggtgttttct gatattcaag agtgaagttg
     2761 taagaaggaa cagcaatctt gatgacttta agccatgagt tcattacaga attagttaat
     2821 tgtctgaaat ctctcgtctt taaatcttct tctattcaga tcctgttttc caatatttta
     2881 actaattgtt cgtgcgacag ttttaccggg ttgtttttgt tcgatgtttt tgatgcaatt
     2941 gattctaagt cattcccttt tactccaaat tgtcccagct tcggtaattt caatttttcg
     3001 gtaagctgtt caagataatc aataaaatat tccgctaaga aatagttcgg tttaccttcg
     3061 tactttccta taattctgcc taatgttgca tatttctgta aggaaatccc ttgttcgttc
     3121 gattttttta gttcctgaag ggttattttg ttcgtttctg ccataagcga cccgcaaact
     3181 gccccatggg gaatttcggt agttgctccc accgatgagg caaatccatg aactgtaccc
     3241 aatccggcat tggcaagcgt gattcctgag agccatgctg cgtaggccat ttttgagcgc
     3301 aggaggagat tggtaggttg gcagagaacg agttcttcca gagattgaac gatatgcata
     3361 attccgtcga gggccagggt gtcggtcatc gggtttgcgt tggttgacag gtacgattct
     3421 acgagctgag aaaaggcatc cattccgcta caagcggtta gactagccgg gcaggtaagc
     3481 tgcaattcag ggtcgatgat ggccacattg ggaatatagt tgtcgtgtct caacgaactt
     3541 ttgaaacctc cgtttcctac aacagaaatc accgcattct tggtggcttc acttcctgtt
     3601 ccggctgttg ttggaacagc tataaatggg actttcttgc ctgtaggctg agtatcgccc
     3661 acttgttcga ggtaatgaac tataggtaat tctgaaacta gcatggccga taaagctttg
     3721 ccagcatcaa taacactacc gcccccgatg gcaacaacca catcgggcaa ggagtccctg
     3781 taaagctgta ctccttggtc gatatctgag ggagaaggtt catgcccaat catgagttca
     3841 cgccaaagta tgcattgtgt ttttagtctt gaagtaagat gttcataata aaccgaatga
     3901 gaaaatgaat tctctccggt taccagcaaa atattgttgc cccaatgact tattatttcg
     3961 ggtaggtggt tcagttttcc ggctccgaaa tgaaggtcgg gtaggtggct aaacgaaaaa
     4021 tcgaatttca taagaggatt attagtgtgc caataaattg tattttaccc ccttgcgagg
     4081 ttcagccatc attgtggcta cttcgtcgcg ccactttagg taatgggctg tttctttatg
     4141 gtttgcagct gattgttcgt cggtgtatgc ttcgtagagc ataaaactgc acgggttact
     4201 gctgttttga caaatgtcga aacgaaggtt gcctggttct ttcaccgaat gaaagtggtt
     4261 ttcgagcgat gctttgataa aatcatcaat tttgtcgggt acgacttgaa catggacaca
     4321 tgttgtaata gccattgtat tattgtttaa gttaaacaaa ttaaaaatcg ttttcctttt
     4381 taaaaagaat ccttaaattt aaagagtaat cttagataaa caggagatga gaagattaat
     4441 tattaccatg ttgttagtaa tgagtagtat ggctcttttt tcacaaaaga gtataaaact
     4501 gccagagcca aagaaatcgg gtggtatg
//
LOCUS       DAOPUW010000065         4526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_19245_length_4526_cov_3.635428, whole genome shotgun sequence.
ACCESSION   DAOPUW010000065 DAOPUW010000000
VERSION     DAOPUW010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19245_length_4526_cov_3.635428"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PKJ43_01850"
     CDS             <1..593
                     /locus_tag="PKJ43_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNZ71332.1"
                     /translation="PEIFDGLITIKKIVRVPGQRAKVAVESYDQRIDPVGACVGMKGS
                     RIHGIVRELRNENIDVINFTNNITLYIQRALNPAKITSIKINEASKTAEVYLKPEEVS
                     LAIGKGGLNIKLASQLTGYEIDVYREMQADDEDVNLDEFADEIEDWVIDQLKAIGCDT
                     AKNVLNISRADLIKRTDLEEETIDEVLKILKSEFES"
     gene            649..3699
                     /gene="infB"
                     /locus_tag="PKJ43_01855"
     CDS             649..3699
                     /gene="infB"
                     /locus_tag="PKJ43_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010421736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNZ71333.1"
                     /translation="MVSGTGTQRLGKIAREFNVGIQTIAEFLSKKGYEVESNPNAKID
                     DNMYSVLQKEYSKDIDLKKESEKLNLRNIRHQKNETLSIEDVAETKSDVEEEHDDDDV
                     VIITDHSLKKVPTHTAPKEREVEEIKNETPKKEIKIVGKIDLNKKPEPPRQEPVKEQP
                     KPVEKINKEVPKAVVEKPVQQEKEEKPVSIQPKPQQPQEPVKPVETKIPEKKENVVEV
                     KKAQPVQNISTGEIATVIPKVESDLKVVGRIDLETINQRTRPPKKSRKQREDERKARE
                     QQKRKEFSRPETPSVPAKNEISNRPERLSGPTIVGKITLPDKKQEGKPDSPEEAARKR
                     KRKRIQKERVDINAGAANSGGEKRDVKKPAPGGKFQPKTVVKKRPFHQEISEEDVSKQ
                     VKDTLARLTTKTKSKTSKYRREKRAAFSEKLAHEEAMALQDHNIIKVTEFVSVNELAT
                     MMDIPVTQIISTCMSLGLFVSINQRLDAETMSLVAEEFGYKVEFISADVMAAIEDEAD
                     SADDLLHRPPIVTVMGHVDHGKTSLLDYIRKANVIAGEAGGITQHIGAYNVELSDGRK
                     ITFLDTPGHEAFTAMRARGAQVTDIAIIIVAADDNVMPQTVEAINHASAAGVPIVFAI
                     NKIDKPGADPEAIKNKLAQMNYLVEEWGGKYQSHDISAKSGIGVYELLEKVLLEAEML
                     ELKANPDRRATGSVIESSLDKGRGYVATVLVQNGTLKEGDVLLAGPYMGHVKAMFNER
                     NQRIKQVGPAEPALVLGLNGAPQAGDKFNVMESEREARAIANKREQLQREQGMRTQKH
                     ITLDEIGRRIAIGNFQELNVIVKGDVDGSIEALSDSLIKLSTEEIQVNVKHKAVGQIS
                     ESDVMLASASDAIIVGFQVRPSMAARKLAEQEQIDIRLYSIIYDAIEEIRAAMEGMLS
                     PEMKEEITGTAEILEVYKITKVGTVAGCLVRDGKISRNNKVRVIRDGIVIYSGDLGTL
                     KRFKDDVKDVSSGYECGMNINNFNDIKAGDFIESYKIVEVAKKL"
     gene            3874..>4526
                     /locus_tag="PKJ43_01860"
     CDS             3874..>4526
                     /locus_tag="PKJ43_01860"
                     /inference="COORDINATES: protein motif:HMM:NF027659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4968 domain-containing protein"
                     /protein_id="HNZ71334.1"
                     /translation="MKTILWLSIFFFLATSSFGRSLYKIEGNNVIVDLDGIGMKSRML
                     KVEVLSDKAIKIVSGMETSFSTFPDYIFTTPVIPQKFKVGYAQNNIEITTRDLLISVQ
                     EDGLVRIFNREGNKLLIESDRFFEAKNSGDAKYKIKQRFFLNVHEDIYGFGFGEEAPR
                     YNIRGKSFEMKKSLSSIASPVIYSEKGYAIVWNNYSHSFFNDQKSGMEVSSDFANEI"
BASE COUNT         1528 a          859 c         1001 g         1138 t
ORIGIN      
        1 tacccgaaat ttttgatggc ttgataacca ttaagaaaat tgtaagggtt ccgggccagc
       61 gggcaaaagt tgccgttgaa tcgtacgacc aacgtatcga cccggtaggc gcctgtgtgg
      121 gaatgaaagg ttccaggatt cacggcattg ttcgtgaatt gcgcaacgaa aacatcgacg
      181 tgataaattt tacgaataat attacacttt atatacaacg cgcccttaat ccagcaaaaa
      241 ttaccagcat caagataaac gaagcatcaa aaactgccga agtttacttg aagcccgaag
      301 aagtttcgct ggcgatagga aaaggcggtt taaacattaa attggcaagc cagctaaccg
      361 ggtacgaaat tgatgtatac cgcgaaatgc aagccgacga cgaagatgtc aacctcgacg
      421 aatttgccga cgaaattgaa gactgggtaa tagaccagct caaggcaatt ggttgtgaca
      481 cagcaaagaa tgtgttgaac atctcacgtg ccgatttaat taaacgcacc gaccttgaag
      541 aagagacaat cgacgaagta ttaaaaattt taaaatcaga atttgaatcg taataaattt
      601 tgggcgggtt tttttgagaa caagattaaa attttcgaaa ggttgaatat ggtatcaggc
      661 acaggaacac aacggctcgg caaaatagcc cgggaattta acgtaggcat tcaaaccatc
      721 gctgaattct taagcaaaaa aggctatgag gttgaatcaa accccaatgc aaagattgat
      781 gataacatgt attctgtttt gcaaaaagaa tacagtaaag acatcgattt gaaaaaagag
      841 tcggaaaaat tgaatttaag gaacatccgt catcaaaaaa atgaaactct ttctattgaa
      901 gatgtcgccg aaacaaaaag tgatgtcgaa gaagaacatg atgacgatga tgttgtgatc
      961 attaccgatc acagccttaa aaaagttcct actcacacag ctccaaaaga aagagaagtt
     1021 gaagaaatta agaatgaaac acctaagaag gaaataaaga ttgttgggaa aattgatctt
     1081 aacaagaagc ccgagccacc acggcaagaa ccggtaaaag aacagccaaa gcccgttgaa
     1141 aagatcaaca aggaagtgcc taaagcggtt gttgaaaaac ctgttcaaca agaaaaagag
     1201 gaaaaaccag ttagcataca acctaaacca caacaacctc aggagcctgt caagcccgtt
     1261 gagaccaaga ttcccgaaaa aaaggaaaat gtggttgaag ttaagaaagc tcagccagtt
     1321 caaaatattt ctactggaga aatagctact gttattccta aggttgaaag tgatctgaaa
     1381 gttgttggac gcatagacct tgaaacaatt aaccagcgta cccgacctcc taagaaatcg
     1441 cgtaagcagc gcgaagatga aaggaaagca cgggaacaac aaaagagaaa agaattttca
     1501 aggcccgaaa ctccttcggt acctgcaaag aatgagatat caaaccgtcc ggagcgcctt
     1561 agcggaccaa cgattgttgg taaaattacc cttcccgata aaaaacagga aggaaaaccc
     1621 gattcaccgg aagaagctgc aaggaaacgc aaacgcaaac gaattcagaa agagcgcgtc
     1681 gatattaacg caggagcagc caattcgggt ggtgaaaaac gcgatgttaa aaagcctgct
     1741 cccggtggaa aattccaacc gaaaactgtg gttaagaaac gcccgttcca tcaagaaatc
     1801 tcagaagaag acgtttcgaa acaagttaaa gataccttgg ccaggttaac caccaaaacc
     1861 aaatcgaaaa cttcgaaata ccgccgcgaa aaacgtgcag catttagcga aaagctcgct
     1921 cacgaagaag ctatggcatt gcaggatcac aacattatta aagttactga atttgtttca
     1981 gtgaacgaat tggccaccat gatggatatt cctgtgaccc agattatttc cacatgtatg
     2041 tcgctggggt tatttgtatc gatcaatcaa cgcctcgatg cagaaaccat gtctcttgtt
     2101 gccgaagagt ttgggtataa ggtagaattt atcagtgccg atgttatggc agccattgaa
     2161 gatgaggctg atagtgcaga cgatttatta caccggccgc ctattgttac cgtaatgggg
     2221 catgtcgacc acggaaaaac ttcgttgctt gactatatca gaaaagccaa cgttattgca
     2281 ggcgaagccg gaggtattac ccaacatatt ggagcatata atgttgaact aagcgatgga
     2341 agaaaaatta ccttcctcga tactcctggt cacgaagcct ttacggcaat gcgtgcccgt
     2401 ggtgcccagg taaccgatat tgcaattatt attgttgctg ccgacgacaa tgttatgcca
     2461 caaaccgttg aagcgattaa ccacgcctca gctgctggtg tgcccattgt ttttgccatc
     2521 aataaaatag ataaaccagg tgcagacccc gaagccataa aaaataaatt ggctcaaatg
     2581 aattatttgg tagaagaatg ggggggtaaa tatcaatcac acgatatttc tgcaaaatca
     2641 ggaattggag tttatgagct tcttgaaaaa gttcttctcg aagcagaaat gcttgagctg
     2701 aaagccaatc cagatcgtcg tgcaacaggt tcagttattg aatcgtcgct cgacaaaggc
     2761 cgtggttatg tggctactgt gcttgtacag aacggtacct taaaagaagg cgacgtgtta
     2821 ttggctggtc cttatatggg gcatgttaag gcaatgttca acgaacgtaa ccaacggatt
     2881 aaacaggttg gccctgctga accggctttg gtgttggggt tgaatggagc tcctcaggcc
     2941 ggtgataagt ttaacgtaat ggaaagtgaa cgggaagcac gtgctattgc caacaaacgc
     3001 gaacagttac agcgcgaaca gggaatgagg actcaaaaac acattaccct tgatgaaata
     3061 ggccgccgta ttgctatagg caacttccag gaacttaatg ttattgttaa gggtgacgtt
     3121 gatggatcta ttgaagccct ttccgattcg ctgatcaaac tttcgaccga agaaattcag
     3181 gtaaatgtta agcataaagc cgttgggcaa atttcggaat ctgatgttat gcttgcttcg
     3241 gcatccgatg caattattgt cggcttccag gtacgtccat cgatggcagc acggaaactg
     3301 gcagagcagg agcaaatcga catcaggctt tattcaatca tttacgatgc tattgaagaa
     3361 atccgtgcag ctatggaagg tatgctttct cctgaaatga aagaggaaat taccggaacg
     3421 gccgaaatac ttgaagtata caaaattact aaagttggaa cagttgcagg ttgcttagtc
     3481 cgcgatggaa aaataagccg gaacaacaaa gtacgcgtta tccgggatgg tattgtgatc
     3541 tattccggcg accttggaac cttgaaacgt ttcaaagatg acgtgaaaga tgtttcatct
     3601 gggtatgaat gcggtatgaa tatcaacaat ttcaacgata ttaaagcagg agactttatt
     3661 gagtcataca aaattgttga ggtagccaag aaattgtaat ttttagcaca atatttgaag
     3721 cctgtgaaat caaaagtttt gcaggctttt ttattttcat aaaatagtct tattttgtaa
     3781 aaggttaatt agaaaaatta acgggtaaat aaagcaacag aaatatttca ttatttttta
     3841 caaatatttc tgaccagtaa aaacgatgaa acgatgaaaa caattctttg gttgtcaata
     3901 tttttcttcc ttgctacatc ttcattcgga agatcgttgt ataaaattga aggtaacaat
     3961 gtcatcgtcg atttagatgg tattggaatg aagtcgagaa tgctaaaagt tgaggttttg
     4021 tctgacaaag ccatcaaaat agtatcaggt atggaaacct cattttccac ttttcccgat
     4081 tatattttca ctacccctgt aatacctcag aagtttaaag ttggctatgc ccaaaacaat
     4141 attgaaatta ccacccgaga cctgcttatc agcgttcagg aagacggcct ggtgcgaatt
     4201 tttaatcgcg aaggaaataa gcttcttatc gagtcggatc gtttttttga agccaaaaat
     4261 tcaggtgacg cgaaatacaa aattaagcaa cgttttttcc taaatgttca cgaagacatt
     4321 tacgggtttg gattcggaga agaagcacca cggtacaata ttcgaggaaa atcgtttgag
     4381 atgaagaaat cactatcatc tattgcttct cctgtaattt attcagaaaa ggggtatgcc
     4441 attgtttgga ataattattc gcattctttt ttcaatgacc agaaaagtgg catggaggtg
     4501 tcttccgatt ttgccaacga aattca
//
LOCUS       DAOPUW010000066         4484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_19485_length_4484_cov_3.517724, whole genome shotgun sequence.
ACCESSION   DAOPUW010000066 DAOPUW010000000
VERSION     DAOPUW010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19485_length_4484_cov_3.517724"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /gene="pepD"
                     /locus_tag="PKJ43_01865"
     CDS             complement(<1..1326)
                     /gene="pepD"
                     /locus_tag="PKJ43_01865"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HNZ71335.1"
                     /translation="MERNKTLNHLTPNVVWGHFESTCQIPRPSRKEEKIIAFLEEFSN
                     KNKLKFKKDKAGNVLISKAATPGFENKKTVVLQSHLDMVCEKHSYIEHNFETDPITPV
                     IDGEWVKAQGTTLGADCGIGIAAQLAVLTSSNIEHGPIECLFTVDEETGLTGAFALEP
                     GFFDGKILINLDSEDEGELFIGCAGGIDTVATFTFQTREIPAGFFPLEIKVSGLQGGH
                     SGDDINKGRANANKLLVRILWDLYHKYGIKRTHIEGGNLRNAIAREASARFLVLKGYK
                     ENLVAQFNIYVNDLHEELRITEPGVKIELSSAEMPAFVLCDENCEKLLNMLYACPHGV
                     FAMSYRMPGLVETSTNLASVKIKDNRVIEITTSQRSDHESGKLDIMSMVESVFKLGGA
                     TVVHSDGYPGWTPNPDSEILRITEKAYIELFGNKPIVRSIHAGLECGLFL"
     gene            1499..1933
                     /locus_tag="PKJ43_01870"
     CDS             1499..1933
                     /locus_tag="PKJ43_01870"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HNZ71336.1"
                     /translation="MRKAFLMVVLAVVFVFSASAAGIEGKWKTIDDETGKEKSVVELT
                     IKDGKLYGKIVKLFNPDPNYDPVCTECKDHLKGKKKIGMQIVNGLSLAEGKWARDKGI
                     LDPENGKYYDVKMWVDEKDASKLNVRGYIGFLYRTQTWIRVE"
     gene            complement(2238..4349)
                     /locus_tag="PKJ43_01875"
     CDS             complement(2238..4349)
                     /locus_tag="PKJ43_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HNZ71337.1"
                     /translation="MNRILQIAHILIIALTCSILGFSQEPYTQLPDTFNRSNPSHFYA
                     YIKQNIQDNNQSKLVIQLIEDGAKLAREKHNDTMLVMMDYFLADYFYYREEYPKAIDS
                     YQYVLPKFEQLHDTLMIARTFNAMGLAYGYQSDENNQLENLLKANEILSKTPSKKRII
                     ELERIIVKTNIINHYRKRGDHEKTINLASTVLDKAREIGDSTRLASILNTVALSYKNL
                     GQTDNALEMFNEAGKIFDKQNNTFHKAFTYINIGGLYDYIHKNDSTLKYYTLALNTFK
                     EVGYAYGELTALTGIAEVYAITNQRNEASKIFISSIERAKELGFSDIVIDSYADLADI
                     EYKNKNFQKAYEYQQKHYELRDSVSSFEKEKKLAELQTQYETVQKENEINLLKSEKLI
                     HENELRRNKLFSWTAFIVIFFLLVFIYVGFIFLNQNKKANELLTEKNNQIEAKNRQLS
                     ELNLQVMEINKNLKKSETALTLANNAKNRFFSILGHDLRNPFHSIMGQSYLLSKSYDQ
                     LSVEERKKYAQDIYQSCEKVNRLLDNLLEWIRTQSEGIVFNPQLTDFQQLVQESIAVL
                     KSNADEKSINIVNKINHKIQITADYTMLETIVRNLIANGIKFTPQGGEVIISARIQNQ
                     HLIVGISDNGVGIAPENMDKLFLVDSNFKTNGTNNERGTGLGLAICKELVSIHHGEIW
                     AESVPGKGSVFHFSIPAPLTR"
BASE COUNT         1133 a          878 c         1020 g         1453 t
ORIGIN      
        1 caggaacaat ccgcattcga gcccggcatg gatagaccgc acaatgggtt tattgccaaa
       61 gagttcaatg tatgcttttt ctgttatccg caatatttcg gagtcaggat ttggcgtcca
      121 gcctggatac ccgtcggaat ggactactgt tgccccgcca agtttgaaaa ccgattcgac
      181 catcgacatg atatcgagtt tcccggattc gtggtcactt cgctggctgg tggttatttc
      241 tattacccga ttgtctttta ttttcaccga agccagattt gttgatgttt caacgagtcc
      301 cggcatgcgg tagctcatgg caaaaacccc gtgcggacag gcatagagca tgttgagtaa
      361 tttttcacag ttttcgtcgc aaagcacaaa ggctggcatc tcagccgatg ataactctat
      421 tttgactccg ggttcagtaa ttcttaattc ttcatgcagg tcatttacat agatgttaaa
      481 ctgggcaacc aggttttctt tgtagccttt cagcacgaga aaacgggcac ttgcttcacg
      541 ggcaatggcg ttgcggaggt ttccgccttc gatgtgggtg cgtttaatgc cgtatttgtg
      601 gtacaggtcc cataaaatcc gaaccagtag tttattggca tttgcccgtc ctttattgat
      661 gtcgtcgccc gagtgcccgc cttgcagacc ggaaactttt atttcgagcg ggaagaaacc
      721 agcaggtatt tcgcgggttt ggaaagtgaa agtcgccacg gtatcaatcc ccccggcaca
      781 accaatgaat aattcccctt catcttccga gtcgaggttg atcagtatct ttccatcgaa
      841 aaagcctggt tcaagggcaa atgcccctgt gaggccggtc tcttcgtcta ccgtaaagag
      901 acactcaatg gggccgtgtt ctatgtttga cgaggtgagc acggcaagtt gtgccgcaat
      961 tcctatcccg cagtcggcac caagggttgt cccctgagct ttaacccatt cgccatcaat
     1021 aactggtgta atggggtcag tctcaaagtt gtgttcaata tagctatgct tttcgcaaac
     1081 catatcgagg tgcgattgaa gcactaccgt ttttttgttt tcaaatcccg gagtggctgc
     1141 tttcgaaatc aatacatttc ctgccttatc ttttttgaat ttcagtttgt tcttgttcga
     1201 aaattcttca agaaaggcaa ttatcttttc ttccttccgc gaagggcgtg gtatttggca
     1261 tgtcgattca aaatgccccc atacaacgtt gggagtaagg tggttgagtg ttttgtttct
     1321 ttccatcgga aatgtatttt ctttattagt agtaaatggt ctctgcctgc aaagttgcaa
     1381 tctttagaga aacaatatac taaaatgaaa agttttatca catttatgct gaacattatt
     1441 tttcggaatg gtgttataag gtcttgtcat attgtttaat aacattaaaa tcgtttgtat
     1501 gagaaaagca tttttgatgg ttgttctggc tgttgtgttt gttttttcag ccagtgcagc
     1561 aggtatcgaa ggaaaatgga aaactattga tgatgaaacg ggaaaagaga aatcggttgt
     1621 agagctaact attaaagacg gtaaacttta cggtaaaata gtgaagcttt tcaatcccga
     1681 tccgaattac gatccggttt gtactgaatg taaagaccat ctgaaaggaa agaaaaaaat
     1741 agggatgcaa attgtgaacg gtctttcact ggccgaaggc aaatgggcac gcgataaggg
     1801 aatcttagac cccgaaaacg gaaagtatta cgatgtgaaa atgtgggttg acgaaaaaga
     1861 tgcatcgaag ctgaatgtac gcggctatat tggtttcttg taccgcactc aaacctggat
     1921 tcgcgttgaa taaatattta ttgttgtccg gtgaagtact ttaattaatt aggagtcaat
     1981 acgctataaa agttgatttc attttttgtg ttttaccgga caacagtgat ttcttgcgct
     2041 ttttacattc taataaacta taggaacatt gcatggacga cgaattgatt gcttgtcagg
     2101 cttgtggtgc ccgttttaag aaggacgatt tgcagcgtag ctgcggcaac tgttttgctt
     2161 gtacggggtg tgaaatatat gtctgtgcag tgtgtaaggc cgaaattgta gtaaggccaa
     2221 tgcggcagat taaaaaatca acgggtgagc ggagcgggaa ttgaaaagtg gaataccgat
     2281 cctttcccgg gcacactttc tgcccatatt tctccatggt gtatgctgac cagttcttta
     2341 caaatggcta agcccagccc ggttccccgc tcgttattcg tgccgttagt tttaaaattt
     2401 gaatcaacaa gaaacagttt atccatattt tcgggggcaa ttccaacccc gttatccgaa
     2461 ataccgacaa taaggtgctg gttttgaatc cgggcactta tgatcacttc gccaccctga
     2521 ggagtaaact ttattccgtt ggcaataagg ttacgcacaa ttgtttcgag cattgtgtaa
     2581 tcggcggtga tttgtatttt atggttgatc ttgtttacaa tgttgattga tttttcatcg
     2641 gcattgcttt tgagcacagc gattgattcc tgaaccagtt gctgaaaatc ggtaagctga
     2701 ggattaaata caatgccttc cgattgggtc cgaatccact ctaaaagatt gtcgagcagg
     2761 cggtttactt tttcgcacga ctgatagata tcctgagcat atttttttcg ttcctctact
     2821 gatagttggt cgtacgactt cgagagcagg tatgactggc ccattatgct gtggaatgga
     2881 ttcctcaggt cgtgccccag aatagagaaa aaccggtttt ttgcattgtt cgccagcgta
     2941 agtgcggtct ccgatttttt gagatttttg tttatctcca taacctgaag gttcagctcg
     3001 cttaactggc ggtttttcgc ttcaatctga ttgttttttt cggttaaaag ctcattggct
     3061 tttttgttct gattgagaaa aatgaacccc acatagataa aaacgaggag gaaaaagatc
     3121 acaataaagg ccgtccacga gaagagtttg ttccgtctca gctcattttc gtggatcaat
     3181 ttttccgatt tcagcaagtt tatctcattt tctttctgaa ccgtttcata ctgggtttgc
     3241 agttcagcca gttttttctc tttctcgaac gacgatacac tgtctctcaa ctcgtaatgc
     3301 ttttgctgat attcgtaggc tttctggaag ttcttatttt tgtattcaat gtcggccaaa
     3361 tcggcatacg agtcaataac gatatcgctg aaccccagtt cttttgcccg ttcaatgctt
     3421 gaaataaata ttttgctggc ctcgttgcgc tggttagtga tcgcatatac ctcggcaata
     3481 ccggttaagg cagtgagttc gccataggca tacccaactt ctttgaacgt gttgagggcc
     3541 agtgtgtaat atttcagcgt tgagtcgttt ttgtggatgt aatcgtaaag tcctcctatg
     3601 ttaatgtagg taaaggcctt gtggaaagta ttattctgtt tgtcaaaaat ctttccggct
     3661 tcgttgaaca tttcaagcgc attgtcggtc tgccccaggt ttttgtatga gagggctacc
     3721 gtgtttagaa tggatgccaa acgggttgag tcgccaattt cgcgtgcttt gtccaatacc
     3781 gtcgaagcca gatttattgt tttttcgtga tcgcctcttt ttctgtaatg gtttattata
     3841 ttggttttta caattatccg ctctagttcg attattcgtt tcttcgatgg tgttttgctt
     3901 aatatttcat tggccttgag caggttttca agctggttgt tctcgtcgct ttggtagcca
     3961 tacgccaggc ccatcgcgtt aaaagtcctg gcaatcatca gggtgtcgtg aagttgctcg
     4021 aatttgggaa gtacatattg gtatgaatcg attgctttag ggtattcttc ccggtagtaa
     4081 aaatagtcgg ccaggaaata atccatcatt acaagcatgg tgtcgttgtg tttttccctg
     4141 gccagttttg ccccatcttc aataagctgg attacgagtt tcgattggtt attatcctga
     4201 atattctgct taatgtaagc gtaaaaatgg gaagggttgc ttctgttaaa ggtatcgggc
     4261 aactgcgtat agggttcctg tgaaaaaccc aatatgctgc atgttaatgc aattatcagt
     4321 atgtgagcta tttgaagtat gcggttcata agctgagaaa ttcaacttta aaagtaacaa
     4381 aaatgctgaa aaggaaaaca attgttatta attcgacttc tttttgtcca aaaaattgta
     4441 tttatttttg aaaaggagac gacattactt atttgtgtaa aatg
//
LOCUS       DAOPUW010000067         4468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_19583_length_4468_cov_3.539995, whole genome shotgun sequence.
ACCESSION   DAOPUW010000067 DAOPUW010000000
VERSION     DAOPUW010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19583_length_4468_cov_3.539995"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="PKJ43_01880"
     CDS             complement(<1..901)
                     /locus_tag="PKJ43_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HNZ71338.1"
                     /translation="MINITLPDGSIKQYDQQVSGLDIANSISPRLAAEVLAVSVNDEV
                     IDLTRPITSDASVKLFKWDDKEGKHAFWHSSAHLMAEAIEALYPGTKFGIGPTIETGF
                     YYDIDLPEGKVITDKELEAIEKKMLELARQKNDIVRSEISKADALKLFEEKGDQYKLE
                     LITDLEDGTITLYKQGNFTDLCRGPHIPNTEPIKAIKLLSVAGAYWRGNEKNKMLTRI
                     YGVTFPKAKLLEEYLLLLEEAKKRDHRKIGKEMDLFAFSPAVGMGLPLWLPKGAMLRE
                     QLEIFLKKVQKKFGYEQVITPHIG"
     gene            complement(1052..2014)
                     /locus_tag="PKJ43_01885"
     CDS             complement(1052..2014)
                     /locus_tag="PKJ43_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNZ71339.1"
                     /translation="MNELSAKKNILVGFFFFLGLAFLIIGILMIGDLHKTFEKKINIV
                     CYFDDVAGLQKGNNVWLSGVKIGTVSELNFYGSSKVKVQFQVDEKTKPYIRKNSKVKL
                     GSDGLIGNRILVIYGGNEETNMIEEGDTLAVEKTFSSEDMLNTLQKNNENILAITDNF
                     KTLSGNLLKTEGTVGKLLNENSVYDNINSATKSLQQASEKAQQLAQSLYQFSSNLNKE
                     GTLVNDLTTDTVVFNSLKTTITQLQQISDSASVFLSSVKNEASNPNSTLGILMSDNET
                     GASLKTTIKNLESSSQKLDEDLEALQHSPLLRRYFKKKQRKDSK"
     gene            complement(2095..2871)
                     /locus_tag="PKJ43_01890"
     CDS             complement(2095..2871)
                     /locus_tag="PKJ43_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ71340.1"
                     /translation="MEKLQKHFINRDEPVISIRGLHKSFEQLDVLQGIDFTLYKGENV
                     AILGKSGTGKSVLIKIIAGLLKPDKGEVFALGKQVDKLKRKELSDLRLRIGFSFQNSA
                     LYDGMNIYQNLAFPLVMNSKNMTRREITIAVERVAEAVGLKDKLSQMPSDLSGGQRKR
                     IGIARTLILKPEIMLYDEPTAGLDPITSTEIIELINSVQRRYNTSSIIITHDLTCAKN
                     TSDRAAMLHDGKFIKVGTFDELFGTNEEPLKEFYDYNFIH"
     gene            complement(2877..3671)
                     /locus_tag="PKJ43_01895"
     CDS             complement(2877..3671)
                     /locus_tag="PKJ43_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ71341.1"
                     /translation="MTLFKKRKYIFTKRLDQLFSDLYSTVRFILLFLKDGFSRPFHFK
                     SILHQCYEIGIKSLPLITLTGFIVGMVFTKQARPSLEDFGATSWLPSLMGIATIKALG
                     PLVTALICAGKIGSGIGAELGSMKITEQIEAMEVSSTAPFKYLVVTRIWATTVSITIL
                     VFYCSFLMLLGSYFNVHTEESSSMLTYFQNAFSTITFLDIFALIFKSVLYGFTIGIVG
                     CYKGYHARQGTVGVGRAANQAVVTSMFLIFIEEVLVVQVAHWIRYF"
     gene            4019..>4468
                     /locus_tag="PKJ43_01900"
     CDS             4019..>4468
                     /locus_tag="PKJ43_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNZ71342.1"
                     /translation="MKHFFVLLVALSVLYGVHAQSVKQLSLEDFVVKYTFQQETVSGI
                     RSMNDGLHFTTLKDNKQIVKYSYKTGDQVAVILDLATIAECPINTIENYTFSNDERRI
                     LLQTNKKRIYRHSYTAEFYIWDIQTRSISALSDKGPQQVPLFSPDGER"
BASE COUNT         1155 a          904 c          927 g         1482 t
ORIGIN      
        1 cgccaatgtg cggggttata acttgttcat aaccaaattt cttttgtact tttttgagga
       61 aaatctcgag ttgttcgcgt agcatagctc ctttagggag ccaaagcgga agacccatcc
      121 ctactgcggg tgagaaggcg aaaagatcca tctctttgcc aattttccgg tggtcgcgtt
      181 tcttggcttc ctcaagcaat aggagatatt cttccagtag ttttgctttc gggaaggtaa
      241 ccccgtaaat gcgggtcaac attttgttct tttcattacc acgccagtaa gcaccagcca
      301 cactcaacag cttgatggct ttgatgggct cggtattggg aatgtgcggg ccacggcaca
      361 agtcggtaaa atttccttgt ttgtataagg tgatggttcc gtcttccaaa tccgtgatca
      421 gttccagttt gtactgatcg cctttctctt cgaaaagttt cagcgcatcg gcctttgaga
      481 tttcggaacg gacaatatcg ttcttctggc gggcaagttc gagcattttt ttctcgattg
      541 cttccagttc tttatcagta attaccttac cttcaggaag atcaatatcg taatagaaac
      601 cagtttcgat ggttggccca attccgaatt tggttccggg ataaagggct tcgatggctt
      661 cggccatcaa gtgagccgac gaatgccaga aagcgtgttt gccttctttg tcgtcccatt
      721 tgaacaactt aaccgatgca tcggatgtga tgggacgggt aaggtcaatt acttcgtcgt
      781 ttactgaaac tgccagtact tcggcggcca aacgcggact gattgaattc gcaatatcaa
      841 gaccgctaac ctgctggtca tattgtttaa tacttccgtc aggaagagta atattgatca
      901 tctttcaaat tttttaacaa ggttgcaaag ataattataa tctaaaaaat acaatgaaac
      961 ttttacagta tcttctaatt tgctgaatac ggtaatttgt tcagttccat gcaccttgat
     1021 gtagacttaa agaattgcgg tattgcatct cttatttgct gtcttttctt tgtttcttct
     1081 taaaatatct ccggagcaac ggactgtgtt gcagggcttc aaggtcttca tcgagttttt
     1141 gagagctgct ttcgaggttc ttgatcgtgg ttttaaggct ggctcctgtt tcattgtcgc
     1201 tcattaatat tcccaatgtt gaattgggat tgctggcttc attcttgact gaactaagaa
     1261 aaaccgacgc tgagtctgat atttgttgca gttgggtaat tgtcgttttt aatgaattga
     1321 aaactaccgt gtcggtggtg agatcgttaa caagcgtgcc ttccttgtta aggttgctgc
     1381 tgaattgata caggctttgt gcaagttgtt gtgctttttc cgaagcttgc tgcagcgatt
     1441 ttgttgcact gttgatgttg tcgtaaacac tgttttcgtt tagcagtttg cccacggtgc
     1501 cttcggtttt gagcaaatta ccactgaggg ttttgaaatt atccgtgatg gctagtatgt
     1561 tctcgttgtt tttctgcagg gtgttaagca tatcttccga tgaaaaggtc ttttcaacag
     1621 ccagagtatc accttcttca atcatattgg tttcttcgtt cccgccatat atcacaagga
     1681 tacggttgcc aataagcccg tcgctgccca gtttaacctt cgagtttttg cgaatgtacg
     1741 gtttggtttt ttcatcgact tgaaactgta cttttacttt cgagcttccg taaaaattaa
     1801 gttcgcttac ggtgccaatt tttactcccg acagccaaac gttgttgcct ttttgcaagc
     1861 cggcaacgtc atcgaaatag caaacgatat ttattttctt ctcgaaagtt ttgtgcaagt
     1921 cgccaatcat caggattcct ataatgagaa atgccagtcc taaaaagaaa aaaaatccaa
     1981 ctaaaatatt tttttttgcc gataattcat tcatcgttta tattgttttt gatgcgttaa
     2041 ttttttgatt tatagcgttt tttgacgcac ctttttgttt gttaataact gctttcagtg
     2101 tatgaaatta taatcgtaaa actcttttaa tggttcttca tttgttccga ataactcgtc
     2161 aaatgtacct actttaataa atttcccatc gtgtagcatt gccgcacggt ctgatgtgtt
     2221 tttggcacag gtcaggtcgt gtgtaatgat aattgagctg gtgttgtacc tgcgttgtac
     2281 gctgtttatc agctcaatga tttcagtgct tgtaattggg tcgaggccgg ctgtaggctc
     2341 gtcgtagagc ataatttccg gcttaagtat aagagtgcgg gcaattccta ttcgtttgcg
     2401 ctgacctccg gaaaggtctg atggcatttg acttaactta tcctttaacc caacagcttc
     2461 ggcaactctt tcaacggcga tggttatttc gcggcgggtc atgttttttg agttcatgac
     2521 aagcgggaag gcaaggtttt ggtaaatgtt catgccatcg taaagtgcac tgttctggaa
     2581 tgaaaaacca atcctcaggc ggaggtcact tagttctttc cgttttagtt tgtctacttg
     2641 ttttcccaac gcaaaaactt cgcctttgtc gggtttcaaa agcccggcta ttattttaat
     2701 caataccgat ttcccggtac ctgatttgcc aagtatcgca acattttcac ctttgtataa
     2761 tgtaaaatcg atcccttgca acacatccag ttgctcaaat gatttgtgca ggcccctgat
     2821 cgaaattaca ggctcatcgc ggttgatgaa gtgtttttgt agtttttcca tggctattaa
     2881 aagtacctga tccaatgtgc aacttgcaca acaagaactt cttcaatgaa aattaaaaac
     2941 atacttgtaa caacggcttg gtttgcagcg cgtccaacac caacagttcc ctgtctggca
     3001 tgatatcctt tatagcatcc cacaatacct attgtaaacc cgtataatac tgatttaaat
     3061 atcaaggcaa atatatcgag aaatgttatt gtggaaaaag cattctgaaa atacgtcagc
     3121 atactgctcg attcttcagt gtgaacattg aaatacgacc cgagcaacat taaaaaactg
     3181 caataaaaaa ccaaaatagt tattgaaacg gtggtcgccc atattcttgt tacaacaagg
     3241 tacttaaatg gggctgtcga tgaaacttcc atagcttcaa tctgttcggt aattttcatt
     3301 gaacccagct cagccccgat tccagagcca atttttcctg cacaaattaa ggcagtaacc
     3361 aaaggtccca gtgctttaat ggtagcaatg cccattagcg agggcaacca ggaagtggct
     3421 ccaaaatctt cgagcgacgg gcgggcttgt ttagtgaaaa ccattcccac tataaaaccg
     3481 gttaatgtaa tcagaggcag ggatttaatg cctatttcat agcattgatg cagtattgac
     3541 ttgaagtgga atgggcggga aaaaccatct ttcagaaaaa gcaaaataaa ccgaactgta
     3601 ctgtacaaat cggaaaaaag ctggtcgagc cgctttgtga agatatattt gcgcttctta
     3661 aaaagtgtca tttattcatt cgctggtttt ccttttcttg cagtaaaggc tgccaaaagt
     3721 atatctattt ttttaaaagt ctccttttat tggggtgttc tggtacaaaa ataaacattt
     3781 ctcaaccgtg attttttatt tctgccatgt ataatattat gtttatcgat agtttttgta
     3841 gctgtttttc tttctttatt aatgttgtat cgaacatgtt tagtattgag gcatcgtatt
     3901 tcaagcttat tacagttgca caatgaattg aagcgttgac cgttcttgtt gcatggcttc
     3961 atttgcctaa agtcggaaat aatcgtattt ttcggcctga atttaacctg tatttgccat
     4021 gaagcatttt tttgttttgt tagtagcctt gtccgtattg tatggtgttc atgcccagtc
     4081 ggtcaagcag ttatctcttg aagatttcgt tgttaagtat acgtttcagc aagaaacagt
     4141 ttcggggatc cgctcgatga acgatgggct gcattttacc acgctcaaag acaataagca
     4201 aattgttaag tatagttaca agaccggcga tcaggtagcg gttattctcg atttggcgac
     4261 cattgccgaa tgcccgatca atacaataga aaattatact tttagtaacg acgaacgccg
     4321 catccttctc caaacgaata agaaacggat ttacaggcat tcttatactg ccgaatttta
     4381 tatctgggac attcaaaccc gttccatttc ggcgctatcc gacaaaggtc cgcaacaggt
     4441 tcctctgttt tcgcctgatg gtgaacgt
//
LOCUS       DAOPUW010000068         4465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_19611_length_4465_cov_3.422902, whole genome shotgun sequence.
ACCESSION   DAOPUW010000068 DAOPUW010000000
VERSION     DAOPUW010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19611_length_4465_cov_3.422902"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..2968)
                     /locus_tag="PKJ43_01905"
     CDS             complement(5..2968)
                     /locus_tag="PKJ43_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ71343.1"
                     /translation="MTREREQNHPFRIWQKNKFIVVNITHGSMHVLSFFASVLLFVVM
                     FFHPVFSTAQTTLKGIADGVAPIKIVQQSEIDYLFIEDPAPDLPLEGRIPILFVHGWN
                     FNGAPAPPGGDAFVAYAGFFLADNELSRFYKPYIVRYWSNALPVTELAGLLRDKMDIM
                     GLNEKPYVLLAHSMGGLISRSLMNEHIFTRGKYAGITCGELARLLITLGTPHHGSPMA
                     NYTSRNDRLDPIRDIAMVALLLSIEEFVFSKTPYNKVNRSDLRWDNYDNLMNYSKYPD
                     EKNVWLTNLNSYTLFDSKLVCYAGNIAGELVPPPYDNTDKQYKMAAYVQRRIFNFNND
                     GIVPRQSALFDGHLPKKTRYFENYDHAQIVRGKTGETILQDSLRKDLLEVVPPLLVSP
                     SQENIIVKHGELFEIQWANPSPVELVNIYFSANNGLTFTKIASNIDAQIQSYQWQVPD
                     TNSVNCIIRVEKAGEEGLFSQSEQPFTIYHNKLDVKNPVAGNYLVQERENTISWEQSG
                     VASGVKITYHDEKNGISKVINENFPAVNGLNTFSWMPEMPLPASDSVFISFEMLDARH
                     GSDTENYIFNSAHFMMLDFPKVTFLSPVENPVDFDGKEGEKLDINNNYTIGYETEGEI
                     KFVKISLCDSSKNIIEIVRRKNHTPGIKSTNSISWRVPELYGDKYFLYIEAGLRESEI
                     TCSAYTPYSFRINKSLELLSPLPNDRDISCLPCFSVVPLSGATSYYFEICDSISGGKD
                     YLKSFSSEDGEVCFSNSVTDELVAGSTYRLTAWAWIDSLKSYPLQTFFSTSAQKPQLF
                     SLLSPAVDDTLWSERVEFSWERAVGANDYLLQLFHEDNLLFSKELARTDTVFVLSMAP
                     EGYRDTIFVNVSARNTWGKQIVESWFFNKDRVTTPHLMFENASFGLSCFPNPMLNNAE
                     ITFTLPEANCRLKVDLSLFSIEGKVASSLFDGMLNGGKHAFEINTSQLQLPAGVYFLL
                     LNVDGKREVKKIAIN"
     gene            3106..4197
                     /locus_tag="PKJ43_01910"
     CDS             3106..4197
                     /locus_tag="PKJ43_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71344.1"
                     /translation="MKTKILSAVCYFALISALSLQSCYEKPSKKYTKGYFPDSVCNLE
                     IVNSIYDEINMDLRLLRCTNFLTFASNRNTQGGSYDLNSKIFTFIWNQENGKFDAADN
                     LYGQDELYNKMAEATRTDANEYGPTFYRRLIKNEQNNNQFIRYYLFYSDDQKGTLDLR
                     MLTFDEKGTYSENLISELKDKIVDLSFASNQQFNEGYFTLLTEDFSSFLPSGIEAETS
                     IFQLVFCTDETDNYDLYKIDIPQNENLDSFLLQASENGKTPITELNSAQDDRCPSITS
                     NLMVFSSNRPGGLGGYDFYYSILKDGKWSTPVNFGEPINSPYDEYRARVVKAEEYEND
                     VLIFSSNRPGGNGGYDLYYTGIELMREHK"
     gene            4283..>4465
                     /locus_tag="PKJ43_01915"
     CDS             4283..>4465
                     /locus_tag="PKJ43_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775816.1"
                     /note="Sms; stabilizes the strand-invasion intermediate
                     during the DNA repair; involved in recombination of donor
                     DNA and plays an important role in DNA damage repair after
                     exposure to mutagenic agents; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNZ71345.1"
                     /translation="MAKTKTIYVCQACGVTSPKWVGRCPSCGEWNTYIEELEVTGGKS
                     TSSPANISLLQKPLMLS"
BASE COUNT         1453 a          941 c          843 g         1228 t
ORIGIN      
        1 ataatcaatt tatcgctatt tttttcactt cacgttttcc atctacattc aatagtaaga
       61 aatacacacc ggcaggaagt tgaagttggc tggtgttaat ttcaaaagca tgttttcccc
      121 cattgagcat tccatcaaat aaactggatg caacttttcc ttcaatagaa aacaatgaca
      181 gatcaacctt aagccgacaa tttgcttcag gcaaggtaaa agtaatttca gcattattca
      241 acatagggtt aggaaaacac gataacccaa atgaagcatt ttcgaacatc aaatgaggtg
      301 tagtaacccg atccttgtta aaaaaccagc tttcaacgat ttgcttgccc caagtattcc
      361 tggctgatac atttacaaaa atagtatcac ggtaaccctc cggagccatc gacaatacaa
      421 aaacagtatc ggtacgggca agttctttcg aaaacaaaag gttgtcctca tgaaacaact
      481 gtagcaaata atcgttggcg ccaacagccc tctcccacga aaactcaacc cgttcactcc
      541 acaaagtatc gtctacagca ggacttaaaa gactgaacaa ttgaggtttt tgagccgagg
      601 ttgaaaagaa tgtttgcaaa gggtatgatt ttaatgaatc aatccaggcc catgcagtaa
      661 gccgataggt actccccgca acaagttcgt cggtaactga attggagaaa cacacctctc
      721 catcttccga agaaaatgat tttaaataat cctttccacc cgatattgaa tcacaaattt
      781 cgaagtaata cgatgtcgct ccactgagcg gaactaccga aaaacatggc aaacaagaaa
      841 tatcgcgatc attaggtaga ggcgataaaa gttcaagcga tttattgatc ctgaaagaat
      901 acggggtgta agcactgcaa gttatttcgc tttcgcgcag gccagcttcg atatatagga
      961 aatacttatc gccatacaac tcaggtactc tccacgaaat agaatttgtt gattttatgc
     1021 cgggggtatg gtttttacga cgaactattt caatgatgtt ttttgacgaa tcgcaaaggc
     1081 taatcttcac gaacttaatt tctccttctg tttcatatcc aatagtgtaa ttgttgttta
     1141 tatcgagctt ttccccttcc tttccatcaa aatctacagg attctcaaca ggagacagaa
     1201 aggtaacctt tggaaaatca agcatcataa aatgagctga gttaaatata taattttcgg
     1261 tatcgcttcc atgccgggca tccagcatct cgaaagaaat aaagacagaa tcgctagccg
     1321 gaagcggcat ttcaggcatc caggaaaaag tgttcaaccc attaacggca gggaagtttt
     1381 cgttgatcac ctttgaaatt ccattttttt cgtcgtgata cgtaatttta acaccgctgg
     1441 caaccccact ttgctcccag gaaatagtat tttctctttc ctgaacaaga taatttcctg
     1501 caacaggatt tttaacatcg agtttgttgt gatatattgt gaacggctgt tctgattgag
     1561 aaaacaaccc ttcttcgccc gctttttcta ccctaataat acagtttacc gaattggtat
     1621 cgggaacttg ccattgatac gactgaattt gagcatcgat attagaggca atttttgtaa
     1681 acgtgagtcc attattggct gaaaaataga tattgaccag ctcaactggc gaaggattcg
     1741 cccactgaat ttcgaacagc tcgccatgtt taacgataat gttttcctgc gaaggcgaaa
     1801 caagcagagg cggaacgacc tcgagcagat ccttccgaag agagtcctga agtattgttt
     1861 cccctgtttt accccgcaca atttgagcat gatcgtaatt ttcgaaatat cgggttttct
     1921 ttggcaaatg tccatcaaaa agagcacttt gccggggcac aatcccatca ttattgaaat
     1981 taaagatccg gcgctgtacg tatgctgcca tcttatactg cttgtcggtg ttatcataag
     2041 gcggcggcac gagctcacct gcaatatttc ctgcataaca aaccaatttt gaatcgaaaa
     2101 gggtatacga attgagattt gtcagccaaa cgtttttttc atcaggatat ttagaataat
     2161 tcataagatt atcatagtta tcccaccgca agtcgcttct attcacttta ttatatggag
     2221 tcttagaaaa aacgaattct tcaatagaca gtaacaacgc aaccatagca atatcgcgaa
     2281 tagggtcaag acgatcgtta cgggaagtgt aattagccat tggagaacca tgatgcggag
     2341 tgcccaatgt aatgagcaac cgggcaagtt ctccacaggt aatccctgca tattttcccc
     2401 gggtgaaaat gtgttcattc atgagagagc gagaaatgag tccgcccata ctatgcgcca
     2461 aaaggacata cggtttttca ttcaacccca ttatatccat tttgtcgcga agcaaaccag
     2521 ccaactcggt cacaggcaaa gcatttgacc aatatcttac aatatatggc ttataaaatc
     2581 tactcaattc attatcggcc agaaaaaaac cggcataagc aacaaaggca tcacctcccg
     2641 gaggtgcagg tgccccatta aaattccagc catggacaaa gagaatggga attcttcctt
     2701 ctaacggcaa atctggagca ggatcttcga taaacaaata atcgatttcg ctctgttgca
     2761 ctattttaat tggtgcaaca ccatcggcaa tgccctttaa tgttgtttgc gctgttgaaa
     2821 aaacaggatg aaaaaacatc acaacaaaca ataacacact ggcaaagaat gacaaaacat
     2881 gcattgaacc atgtgtgata ttcacgacaa taaacttatt tttttgccaa atcctgaatg
     2941 ggtgattttg ttctctttcc cgtgtcatac gaaaggtttt tgtgaagtta cataaatcga
     3001 aggttaaacc aaaacatgaa attgataaag aaaaagcaat ttttatttca catcttcgca
     3061 acctattgcc aaccagatgc atctttaacc caaaaagaca accacatgaa aacaaaaatc
     3121 ctatcagcag tatgctactt cgcactcata tccgcgttaa gtttacaaag ttgttatgaa
     3181 aaaccgagca agaaatacac aaaaggatac ttccccgatt cggtttgcaa tctcgaaatt
     3241 gtcaatagta tttatgacga aatcaacatg gatttgaggt tgttgcgttg cactaacttt
     3301 ctaacattcg cttcgaaccg aaacacccag ggaggttctt acgaccttaa ttcaaaaata
     3361 ttcaccttta tatggaatca ggaaaacgga aaattcgatg cagcagacaa cctttatgga
     3421 caagatgaat tgtataacaa aatggctgaa gctacccgca ccgatgcaaa tgaatatggt
     3481 cctacatttt atcgccgtct gattaaaaat gaacaaaaca acaatcaatt cataagatat
     3541 tatctgttct attccgacga ccaaaaggga acccttgatt tacgaatgct aacttttgat
     3601 gaaaaaggaa cttacagtga aaacttaata tcagaattga aagataaaat tgtcgaccta
     3661 agttttgcta gcaatcaaca atttaacgaa ggttacttta cattgcttac cgaagacttt
     3721 tcttcttttc tgccatcagg cattgaagct gaaacaagca tttttcagct agtattttgt
     3781 accgacgaaa cggacaatta tgacctctat aagattgata ttccacaaaa tgaaaacctc
     3841 gattcgtttt tgttacaagc cagtgaaaat gggaaaaccc caattacaga attaaacagc
     3901 gcacaagacg accgttgtcc gtcaataacc agtaacctaa tggttttcag ctcaaaccga
     3961 ccaggaggtc ttgggggtta cgacttttat tattctattt taaaagatgg gaaatggagc
     4021 acacctgtta attttggaga acccataaat tcaccttacg atgaatacag agccagggta
     4081 gttaaagctg aagaatatga aaacgacgtg cttatttttt catcaaaccg gccgggtggt
     4141 aatggcggat acgacttgta ctacactggt attgagttaa tgcgggagca taaatgatca
     4201 gccagaaagt aaactgcagg gcagcaaatg ctttttaagc gacggaaaac tgcatcatcc
     4261 gaattcttat tatctttggc ttatggcaaa aacaaaaacc atatatgtat gccaggcctg
     4321 tggggtaact tctccaaaat gggttggccg ttgtccgtcg tgcggcgaat ggaacactta
     4381 tatcgaagaa ctggaagtta cgggcggcaa gagcacatca tcgccggcaa atatatcgct
     4441 cctacaaaag ccactgatgt tgagc
//
LOCUS       DAOPUW010000069         4419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_19906_length_4419_cov_3.079056, whole genome shotgun sequence.
ACCESSION   DAOPUW010000069 DAOPUW010000000
VERSION     DAOPUW010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19906_length_4419_cov_3.079056"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PKJ43_01920"
     CDS             complement(<1..316)
                     /locus_tag="PKJ43_01920"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HNZ71346.1"
                     /translation="MNQELGKIADVLLETGALLMGAGANTGRIRVTVTRIAHSLGYQV
                     ELLITHRALMVSVIDDDGEEFTSKLKRTPPHGVNFKVLSGISRMSWRVQDEDWELDRI
                     NEE"
     gene            complement(489..1445)
                     /locus_tag="PKJ43_01925"
     CDS             complement(489..1445)
                     /locus_tag="PKJ43_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HNZ71347.1"
                     /translation="MNRIDRLNAILIQLQSKRIVKAHEIAGRFDISLRTVYRDIRALE
                     EAGIPIGAEAGVGYYLMENFHLPPVMFTHDEAAALLFGEKLIEKMSDEKVKTDFCSAL
                     YKIKAILKPGEKDHLEKLYDRITVFNWNGRDAACNYLYLYEIQQALANKQMLTIEYES
                     KYAEEKTCRTVEPIGLCNYSQRWHLIAWCKLRSDYRDFRGDRIVSLKLIDKFFTEKQH
                     PSVEEYLRLVNPVATEATISILVPKERAKYLDDSKYWYGFVGQEDLGEYVRMQFANDE
                     LHGFAVWMLNTGSLAKVERPAELTAILQSFIKQVVSNYQELL"
     gene            complement(1614..2066)
                     /locus_tag="PKJ43_01930"
     CDS             complement(1614..2066)
                     /locus_tag="PKJ43_01930"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNZ71348.1"
                     /translation="MEKKSVEKTTVLMCSLESSLATLTNDVGTLANDLQKMAGDLNLE
                     IIGPQFWQYTNSDGQPNTKFLLDICIPVKEAKGNPGRFKFVELPVFNCISEIHKGPWN
                     TMMNAYQKIMGEVTRKSIPFSGVSREVYILCDFENQENCITEIQIEIY"
     gene            2331..2948
                     /locus_tag="PKJ43_01935"
     CDS             2331..2948
                     /locus_tag="PKJ43_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71349.1"
                     /translation="MENTIIRNSAKFVSWVFSPLLVPSLGFVLLMNHIPGVEFYSFRL
                     KMVLLAVVVFSSCLLPLLYSLISALNHRLLKTEGNKNNKVMLSLFTALSLFVGAQFLS
                     KLPVAGVFRILLFGACLLTILLFLISFRWNISEHTFSLGGLWGALIALNFRYGMDLVP
                     ALLLISIIAGITASAKIFLEHHSDAEIYTGFVTGASVMFSFLILI"
     gene            complement(2941..3372)
                     /locus_tag="PKJ43_01940"
     CDS             complement(2941..3372)
                     /locus_tag="PKJ43_01940"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNZ71350.1"
                     /translation="MKKSLLIILLLVIFWSCKQKSNDEYAINKPYRKDSAALAPSVLA
                     KADTIQTPEVVFDPSSLRPASADDKYFIVIASFSVEKYALAMKDELEQKGFSPVIVEI
                     NNDGWNKLAISSYKSFSEAEKALTRIRQAKGRFADARLVVK"
     gene            complement(3432..4127)
                     /locus_tag="PKJ43_01945"
     CDS             complement(3432..4127)
                     /locus_tag="PKJ43_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ71351.1"
                     /translation="MSEEKKILLVNDEIDILEFISYNLEKEGYKVYTAQNGKDAIRIA
                     EKKEPDLIILDVMMPEMDGIVTCEEIRKIPKLKSTIIAFLTARSEDYSQIAGFEAGAD
                     DYITKPIRPKVLISRVKALLKRTQAVEEEEKVADDTTINIGSLSIDRERYMISVAGQE
                     MVLPRKEFELLSLLASKPGKVFTREEIYSSVWGEQVIVGDRTIDVHIRKLREKIGEDH
                     IKTLKGIGYKFVD"
     gene            complement(4198..>4419)
                     /locus_tag="PKJ43_01950"
     CDS             complement(4198..>4419)
                     /locus_tag="PKJ43_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ71352.1"
                     /translation="VSDNGIGVDKDHLPRIFERFYRVDKHRSREQGGTGLGLAIVKHI
                     IEAHNQTINVKSQPEVGTTFTFTLQKAVN"
BASE COUNT         1277 a          953 c          810 g         1379 t
ORIGIN      
        1 attcttcatt tatgcgatcg agctcccaat cttcgtcctg aacccgccaa ctcatccggc
       61 taattcccga aagcacttta aaattaacac catgcggagg agtacgtttt agtttacttg
      121 taaactcttc tccgtcatca tcgataacac tgaccattag cgcccggtgc gtaatcagca
      181 gctcaacctg ataaccaagc gaatgtgcaa ttctggttac agttacccga attctacccg
      241 tattagcccc tgcccccata agcaaggccc ctgtttcgag aagcacatca gcaatttttc
      301 ccaattcctg attcataatt caacatctat agatattcaa acaaggtaca aacaaacatc
      361 agtaactgaa agttccgtta accaaaagaa tgtttttcaa aaaaggctgc aagaatacca
      421 aatctatatc aagccgggaa aaattgcacg ttaaggtttc aataaatcgt tatggagaaa
      481 ttttagggtt ataacaattc ctgataattg gaaaccacct gtttgataaa agactgtaaa
      541 attgcagtga gttcagcagg tcgttcaact ttggccagac tacctgtgtt gagcatccaa
      601 acagcaaatc catgaagttc atcgttggca aattgcattc ttacatattc tcccaaatct
      661 tcctgaccaa caaaaccata ccagtatttt gagtcatcga ggtattttgc ccgttctttg
      721 ggcaccaaaa tcgaaatagt tgcttcggtg gccacagggt ttaccaacct caggtattct
      781 tcaaccgatg gatgttgctt ttcagtaaag aatttatcga taagtttgag cgaaactatc
      841 cggtcacccc gaaaatcgcg atagtcggac cttaacttgc accatgcaat aagatgccac
      901 cgctggctgt aattacacaa cccgatgggc tctacagtcc tgcaggtttt ttcttcagca
      961 tatttcgatt cgtattcaat tgtcagcatt tgcttattgg ccaatgcctg ttgtatttca
     1021 taaagataca gatagttaca cgcagcatca cggccattcc aattgaatac ggttatgcgg
     1081 tcgtacaact tttcaagatg gtctttttct ccgggtttca gtatggcttt gattttatat
     1141 agggccgaac aaaaatcggt tttcactttt tcgtcgctca ttttttcaat aagcttttca
     1201 ccaaaaagca atgctgctgc ttcgtcatgg gtaaacataa ctggaggaag atgaaagttt
     1261 tccatcaaat aataaccaac tccggcttca gctcctatag gaatccctgc ttcttcaagt
     1321 gccctaatgt ctcgatacac cgttcgaagg ctaatgtcga aacggccggc aatttcatgt
     1381 gcctttacaa tccgtttact ctgaagttga attaatatgg cattaagcct gtcaatccgg
     1441 ttcattttaa atgcttagaa acaggtttta aaagtagctg aaaaaaagaa gaaacgaaac
     1501 gagacgaagc accgacagaa tcaaaacagg aaacaggcac ccctgaaccg agatcaattg
     1561 accgtaatcc agctgttgcc tgtttcctgc actactcttc ggatagctta taatcagtaa
     1621 atctcaattt gtatttcggt aatacaattc tcctgatttt cgaaatcaca aagaatatac
     1681 acctcacggc ttactcctga aaagggaatt gattttctgg ttacttcgcc cataattttt
     1741 tgataggcat tcatcatcgt attccatggc cccttgtgga tttctgaaat acaattaaaa
     1801 acaggcaatt caacaaattt aaaccggccg gggtttcctt tggcttcttt caccgggata
     1861 caaatgtcga ggagaaattt tgtattgggt tgtccatcag agttggtata ttgccaaaac
     1921 tgaggcccaa taatttcgag gttcaggtcg ccggccattt tttgcaaatc gttggcaagc
     1981 gttccaacat cgtttgttaa tgtagctaag ctcgactcaa gagaacacat tagtacagtg
     2041 gttttttcca cactcttttt ttccatggca ttaatttttt gattggttaa tactaaaaat
     2101 acggttaata gaactacaca aaatgtaggt aatgttttca tggctattct tttaaattaa
     2161 acatcgacac aaaggaaaac ccggggaatg acaacagtgt gtcagtagta aaaaagaaaa
     2221 tcaactttac atggaaaaaa agaaaaataa gtgttacaaa acgccatcga ttcatacaaa
     2281 tatacaagcg atacaaaagg attagcgcat aatttttaca tttgcaaggt atggagaaca
     2341 caattatacg caacagcgcc aaatttgtct catgggtttt cagtccatta ctggttcctt
     2401 cgttgggatt cgtattgtta atgaaccata tacctggagt tgagttttac tctttcagat
     2461 taaaaatggt actgcttgcg gtagtggtat tttcgagttg tttactgccg ttactctatt
     2521 ccctgatttc tgctctgaac caccgccttt taaaaacaga agggaacaag aataacaaag
     2581 taatgctgtc gctctttacc gcattaagcc tttttgttgg tgctcaattt ttatcaaaac
     2641 ttccggttgc aggtgttttc cgaattttat tatttggggc ttgtttgctc actattttac
     2701 tttttcttat ttcttttcgc tggaatattt cggagcacac tttctccctt ggaggcttgt
     2761 ggggggcact tatagctctt aatttcaggt acggaatgga ccttgtaccc gctttacttt
     2821 tgataagcat tattgcagga attactgcaa gtgccaaaat ttttcttgaa caccacagcg
     2881 atgctgaaat ctatacagga ttcgtaactg gtgcgtctgt gatgttttct tttctcatcc
     2941 ttatttaaca accagccggg catcggcaaa gcgccctttc gcctggcgaa tgcgggttaa
     3001 tgccttttcg gcctcgctaa aacttttgta cgaactaatt gccagtttat tccaaccatc
     3061 gttgttaatt tctacaataa ctggtgaaaa acctttttgt tccaattcgt ctttcatggc
     3121 caacgcatat ttttcaacag aaaaactggc aattacaata aagtacttat catctgcact
     3181 ggcaggcctt aatgaagaag ggtcaaacac tacttctggc gtctgaatag tatctgcctt
     3241 tgccaaaaca gaaggtgcca acgctgcact atcttttcta tatggcttat ttatagcata
     3301 ttcatcgtta cttttttgtt tacaactcca aaagataacg agcaaaagaa taattagtaa
     3361 tgattttttc attacgatga acttcataaa tgtttcatag aaacaaggta aggtttttcc
     3421 agtaaaaaaa atcagtcaac aaacttgtaa ccaattcctt ttaaggtttt gatatggtct
     3481 tcgccaattt tctcacgcaa cttacgaata tgaacatcga tggtacggtc gccaacaata
     3541 acctgttcgc cccacaccga cgagtaaatt tcttcgcgtg taaacacttt acctggttta
     3601 gaagcgagca acgaaagcag ttcgaattct tttctcggaa ggaccatttc ctgcccggca
     3661 acagaaatca tataacgctc cctgtcaatc gataagctgc caatattgat ggttgtatcg
     3721 tcggctactt tttcttcctc ttcaacagcc tgtgtccgct tcaataaggc tttcacccta
     3781 ctaataagca ccttaggacg aataggtttt gtaatataat catcggcacc ggcttcaaaa
     3841 ccggcaattt gtgaatagtc ttcgctacga gctgtaagaa aggcaataat agtacttttg
     3901 agtttgggta tcttccgaat ctcttcacag gttactatac cgtccatttc gggcatcatt
     3961 acatcgagaa tgataaggtc gggttctttc ttttcagcaa ttcgaatggc atctttcccg
     4021 ttttgtgcgg tatacacttt atacccttct ttttcaagat tataacttat gaattcgagg
     4081 atatcgattt cgtcattgac caacaatatt ttcttttctt cgctcataat cttcaatttt
     4141 aatagttgat aaagatagaa aatgggtttt acaataaata aacttagtga aaaaaagtca
     4201 attcaccgcc ttttgtaagg taaatgtgaa ggttgtgccc acctcgggct ggctttttac
     4261 gttgatggtt tgattgtgag cctcgataat gtgcttcaca atggccaggc ccagccctgt
     4321 tccgccttgc tcgcgcgagc gatgcttgtc gacacgatag aagcgctcga aaatacgggg
     4381 caaatgatct ttatctaccc ctatcccgtt atcagaaac
//
LOCUS       DAOPUW010000070         4382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_20132_length_4382_cov_3.238965, whole genome shotgun sequence.
ACCESSION   DAOPUW010000070 DAOPUW010000000
VERSION     DAOPUW010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20132_length_4382_cov_3.238965"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..833)
                     /locus_tag="PKJ43_01955"
     CDS             complement(183..833)
                     /locus_tag="PKJ43_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ71353.1"
                     /translation="MKPISVFIVDDHKLFREGIKFALSKKEGFEVIEEASNGSEFIEK
                     IQDVTPDVVLMDIDMPKVNGFEATQFALEHKPTLNILVLSMHADQGHYLKMIELGVKG
                     FILKDSGTEDLLQAITEVSKGNNFFSQELLMNIILKKNDSPTGKALAEKLDISPREFH
                     VLELLCQACSNQEIADKLFISPKTVEGHKAHLMQKTGTQNSVALVLYSIKNHLINL"
     gene            complement(848..2548)
                     /locus_tag="PKJ43_01960"
     CDS             complement(848..2548)
                     /locus_tag="PKJ43_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNZ71354.1"
                     /translation="MESCLKILSLFNIPSDTLRVMSQKKKLSYKHIQGIRENLINIAL
                     VVGAIAAFPVLISSLIRLTTQNLFYLWLPHAGIYLLFLSVVLLRKRLHYNIRTGVMIF
                     GMFSLAIIDLFESGFSGPGFIWLMSSSIITSIFLDLKRTIIALLLSVIAIFTSFFLYS
                     NGIIENGTGINVDSNLINLIITKTLIIILIVSITSFSLRYMYKNLIHTIETLNIQKEN
                     LNQIASTLSKEVESRKSYEEQLVNSERNFRNIFEQSTEAIVIANNQGDIIDYNEAFTK
                     LAGIDNTYASEKDKTAILPSELMNNFKRNWNKIESEGCSNNISYKQDNGILRYLDCTR
                     SIITYNNERAIMLLIRDNTEKLNLEKASYLMAINAEEKERSSFSKELHDGLGPLLSTL
                     KIYLEMYYSSPDDPEIKKRINDTLAESITSVKEISNNLSPYILENLGLTKAVESFVDK
                     LKYSKKIDINFSSDITERCKPEIEIAFFRIITELINNTLKHSHATTIDITLTQETEKI
                     YLKYADNGEGFDFHEVQKRKNGIGLLNLKSRIEKLGGNTEIITSPGSGFKMNVYLVTY
                     "
     gene            complement(2714..3199)
                     /locus_tag="PKJ43_01965"
     CDS             complement(2714..3199)
                     /locus_tag="PKJ43_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ71355.1"
                     /translation="MLVAQTLSKMIEYFGKDVRRINHALKVHNFAALIAKNENLSTEL
                     QELTEVAAILHDIGIPVSEQKYGSCIGKYQELEGPPIAREMLQQLGASPELTERVCFL
                     VGNHHTYTKIDGIDFQILVEADFLVNIFEDEMSTSAIESARDKYFKTKTSSWLLKTMY
                     N"
     gene            complement(3202..3540)
                     /locus_tag="PKJ43_01970"
     CDS             complement(3202..3540)
                     /locus_tag="PKJ43_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNZ71356.1"
                     /translation="MIIKSAKTVEPHQNPHQVDVRRLYDHDNAQVMHMTLQPGQALKP
                     HITPVDVFFYILEGEVDVLVGEETVRVAADNLVESPKDIVHCLSNNTDTVARILVVKS
                     PKPVAKPKLL"
     gene            complement(3586..4131)
                     /locus_tag="PKJ43_01975"
     CDS             complement(3586..4131)
                     /locus_tag="PKJ43_01975"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNZ71357.1"
                     /translation="MNTATQNLEHDHVFILRLTDVMLAMVEKRSTNADHFELVVNLIR
                     KFADGLHHAKEEDLLFPLMGEKGFSPEQGPVAVMLHEHVQGRAYVKGVSEGITALRAG
                     SDGALQTIYENMTGYAQLLQNHIGKENNILFRMADQVFSADEQQELLTQFAKVEANAE
                     TGFDSANSIQKIDDMAAIYLQ"
     gene            complement(4139..>4382)
                     /locus_tag="PKJ43_01980"
     CDS             complement(4139..>4382)
                     /locus_tag="PKJ43_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNZ71358.1"
                     /translation="TAGAKTTINVEISRMNGEHEFAGGAMDKINEITKGYVVPVDGCN
                     TYKVAFKLLEEFEDDLHTHVHLENNILFPKALQLAK"
BASE COUNT         1161 a          834 c          878 g         1509 t
ORIGIN      
        1 attgtcgtcg ataataatga ttttgtattt cctgttttcc atacctaact aaaccaatac
       61 agatgtatgt atggctgatt ggtatgttgt aagggtattt gggtgtttta tttcagggaa
      121 attcactgtt ttttaccaag ggaaaaccct tgtgtgcctt tccggttgaa cttgatagaa
      181 aattataggt ttataagatg attctttatc gagtaaagga caagagcgac cgaattttga
      241 gttcctgttt tttgcattag gtgagcttta tggccttcta cggttttggg gctgatgaat
      301 agtttgtcgg caatttcctg gtttgagcag gcctgacaga gtaattcgag tacatgaaat
      361 tctcttggcg atatgtcaag cttctcggct aatgctttac ctgtgggact atcgttcttt
      421 tttaaaataa tgttcattaa aagttcctga gagaaaaaat tgtttccttt cgatacttca
      481 gttatggctt gtagcaggtc ttcggttcct gaatctttga ggatgaatcc ttttacgccc
      541 aattctatca tctttaaata atggccttga tcggcatgca ttgaaagtac aaggatgttg
      601 agggttggtt tgtgctcaag tgcaaattgt gtcgcctcga atccattgac tttgggcatg
      661 tcaatatcca ttaacactac atcaggcgtt acatcctgta ttttttcgat gaattctgaa
      721 ccattcgacg cttcttctat tacttcaaaa ccttcttttt tgctcaacgc aaatttgatt
      781 ccctcacgga aaagcttgtg atcgtcgaca ataaaaactg aaataggctt catgattttt
      841 acaatggcta atatgtaaca agatacacat tcattttaaa accgcttccc ggcgaggtaa
      901 tgatttctgt attgcctcca agtttctcga tccgactttt taaattaaga agtccaattc
      961 catttttgcg tttttgcact tcatggaaat cgaacccttc gccattatca gcatacttta
     1021 gatatatttt ttcagtttcc tgagttaatg taatgtcgat tgttgttgca tgagagtgtt
     1081 ttaatgtgtt gtttattagt tctgtaatga ttcggaaaaa agcaatctca atttccggtt
     1141 tgcatcgttc ggtaatatca gaactgaagt tgatatcgat ctttttagaa tattttagtt
     1201 tatcaacaaa tgattcaacg gctttagtca gcccaagatt ttcaagaata taggggctta
     1261 agttgttaga aatttcttta accgatgtaa tcgattccgc taatgtgtcg tttattcgct
     1321 tttttatttc cgggtcgtcg gggctggaat agtacatctc tagatatatt tttaaggtcg
     1381 aaagcagagg tccaagaccg tcatgcagtt ctttagaaaa gcttgaacgt tccttctctt
     1441 ctgcatttat cgccattaga taactggctt tttctaagtt tagcttttcg gtattgtccc
     1501 gaatcaaaag cataatcgcc ctttcattat tgtaagtgat tatacttctt gtgcagtcta
     1561 aataacgcaa aatgccgtta tcttgtttgt agcttatgtt gttactgcat ccttccgatt
     1621 caattttgtt ccagtttctc ttgaaattat tcatcaattc tgagggtagt attgctgttt
     1681 tgtctttttc agaagcgtag gtattatcta ttccagccag ctttgtaaat gcttcattat
     1741 aatcgataat atctccctga ttatttgcta tcacaatggc ttcagtgctt tgttcaaaaa
     1801 tgttcctgaa atttctctcg ctgttaacta attgttcctc atagcttttt ctcgattcta
     1861 cttctttact aagcgttgaa gctatttggt ttagattctc tttttgaata tttaatgtct
     1921 caatggtgtg gattagattc ttatacatat atcgtaatga gaatgaagtg attgatacga
     1981 ttaaaatgat gatcagtgtt ttggtgataa tcaaattaat caaattggag tcaacattta
     2041 tgcctgtgcc attttcgata atgccatttg aatagagaaa gaaacttgtg aatatagcta
     2101 tcacagagag taaaagcgcg atgatcgttc gcttcaaatc cagaaaaatg gaagtaatta
     2161 ttgacgatga cataagccaa atgaatccag gaccagaaaa accactttca aaaagatcga
     2221 taattgccaa agaaaacatt ccaaatatca tcacccccgt tcggatattg taatgcagtc
     2281 gtttgcgaag taaaaccacg cttaaaaaca ataagtatat accggcatgt ggtagccaga
     2341 gataaaacag gttttgagtg gtaagcctga tgagtgacga aatcagcacc ggaaatgcgg
     2401 ctatagcccc aacgacaagt gctatattta taaggttttc acgtattcct tgaatgtgtt
     2461 tatacgaaag cttttttttc tggctcataa cccttaatgt atcagatgga atattaaaca
     2521 aactaaggat ctttaagcaa ctttccacaa gtacaaacat cattttttaa cggtgaaaag
     2581 agattttttt tggtttgttc ccttttgatc tgtggaggct ttcgcttaca atatattatg
     2641 ctttcatatt ctgtgtgtta tgaccgtgtt gccgggcaca agtgaattaa tatttgtcag
     2701 gataagtgtt gttttaattg tacattgttt ttaatagcca actgctggtt ttcgttttga
     2761 agtatttatc tcgggcgctt tcgatggccg aagtgctcat ttcatcttca aaaatgttga
     2821 ctaagaaatc ggcttctacc aaaatttgga agtcgatgcc gtcgattttg gtgtacgtgt
     2881 gatgattccc aaccagaaaa cacacgcgct cagtcaattc gggtgaagca ccgagttgtt
     2941 gcaacatttc acgcgcaatt ggagggcctt ccagctcctg gtactttcca atgcacgagc
     3001 cgtatttttg ttccgaaaca ggaataccta tgtcgtgcag gatagctgca acttcggtaa
     3061 gttcttgtaa ttctgtacta agattctcgt ttttagcaat gagtgcagca aaattgtgca
     3121 ccttcagtgc atggttgatc cttcggacat cttttccaaa gtattcaatc attttcgaga
     3181 gggtttgggc aacgagcatc gctacagtaa tttaggtttc gcaacgggtt tgggtgattt
     3241 caccaccaaa atgcgcgcta cggtatcggt gttgttcgac aggcaatgca caatgtcttt
     3301 ggggctttcc accaaattgt cggcagcaac tcgtaccgtt tcttcaccaa ctaacacatc
     3361 aacctcacct tcaagaatgt aaaagaaaac gtcaacgggt gtaatgtgtg gtttcaatgc
     3421 ctgaccgggc tgtaaggtca tgtgcatcac ttgtgcattg tcgtgatcgt aaagtcggcg
     3481 cacatccact tgatgtgggt tttggtgcgg ctcaactgtt tttgctgatt taataatcat
     3541 gataattgat tttggattaa cgatttatga ttaacaatct tgatgttatt gcaagtaaat
     3601 tgccgccata tcgtcgattt tctgaattga attggccgaa tcgaacccgg tttctgcatt
     3661 ggcttccact tttgcaaatt gagttaagag ctcttgctgc tcgtcggcac taaatacttg
     3721 atcggccatt cggaacaaga tgttattctc tttaccaata tggttttgaa gtaattgtgc
     3781 atagccggtc atgttttcat aaatggtctg caacgcgcca tcgcttccag cacgtaaggc
     3841 agtaattcct tcgctaacgc ctttcacata ggcgcgtcct tgcacatgtt cgtgcaacat
     3901 aacggccacg ggaccttgct cgggcgaaaa acccttctcg cccatcaacg ggaacagcaa
     3961 atcttcttct ttggcatggt gaagtccatc ggcaaatttg cgtatcaggt tcactaccaa
     4021 ttcaaaatga tcggcattgg tactccgttt ttcaaccata gccaacatca catcggttag
     4081 cctgagaatg aacacgtggt cgtgttcgag gttttgtgtt gctgtgttca tgttattatt
     4141 atttagcaag ttgtaatgct tttgggaaaa gaatgttgtt ttcgagatga acatgtgtat
     4201 gtaaatcatc ttcaaactct tcgagtaatt tgaaggcaac tttgtacgtg ttgcaaccgt
     4261 cgacaggcac tacgtatcct ttagttattt cattgatttt gtccatagca cctccggcaa
     4321 attcatgctc gccgttcata cgcgaaatct ccacgttgat ggttgttttt gcccccgcgg
     4381 tg
//
LOCUS       DAOPUW010000071         4372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_20191_length_4372_cov_3.519805, whole genome shotgun sequence.
ACCESSION   DAOPUW010000071 DAOPUW010000000
VERSION     DAOPUW010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20191_length_4372_cov_3.519805"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PKJ43_01985"
     CDS             complement(<1..759)
                     /locus_tag="PKJ43_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNZ71359.1"
                     /translation="MGRFFLSILIIALTLGLQAQTNQLKSAKTPKLVVGITIDHLRSD
                     YVTRYWDTFQEGGFKRLFENGAYSLNARLDIHNIRTSTVIPTIYTGSYPAEHGIVGDK
                     WYKHLTKKEIDAINDDFYLTLGSDSKNGNMSANMLKMYTLGDAMKEISNFRSKVYSVA
                     LNPVAAVLSAGHAADGAFWLDPLNGKMISSSYYMENFPDWVMNFNERHLADYYLEQKW
                     EPLLPPSSYKTDFEDAYILEKGFWKKWNTFPYDLS"
     gene            complement(895..1659)
                     /locus_tag="PKJ43_01990"
     CDS             complement(895..1659)
                     /locus_tag="PKJ43_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HNZ71360.1"
                     /translation="MLLVYDGTFEGYLSVVFEVYKRKLEPTEITAKEHFQHTMFTSKE
                     EITTDFDHAERVWKGLQQKLKPELNQIPYSAWLSGEKGIEMALLEYIRQAFILPLSVE
                     GNFGDANILTVRKAARRVMREAMRVLQFVRFQCTLDGIYFSAISPEYDVLPLTIKQLQ
                     NRFADQNWVVYDLKRDYGFYYNKKTSEEITLADKTFSRSTGAMPANLLQEEEGFYQQA
                     WIGYCKSTTIRERLNPKLQRQHMPKRFWKYLTEKKR"
     gene            complement(1685..2998)
                     /locus_tag="PKJ43_01995"
     CDS             complement(1685..2998)
                     /locus_tag="PKJ43_01995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HNZ71361.1"
                     /translation="MNDIVQKKLNILADAAKFDVSCASSGNNRANTQGGIGNAVNCGI
                     CHSFTEDGRCISLLKVLMTNNCIYDCAYCINRRTNDRPRATLTVEELVDLTIGFYRRN
                     YIEGLFLSSGVIKSPDYTMERMMLVAKKLRIEERYNGYIHMKAIPGASSDLIHQAGIW
                     VDRLSVNIEIPSNPELKRLAPEKNYVDIYRPMNFIKENIIAQKEERKHLRHAPTFAPA
                     GQSTQLIVGATPESDRQILSLASDLYQGQRLKRVYYSGYLPVNEYDQRLPAIKTPPLV
                     RENRLYQSDWLMRFYKFKANEIVSDEHPFLDLEIDPKLAWALRNLHQFPINVNKAPYE
                     MILRVPGIGVQSAQRIIEARKFRTLNTQHLTKIGVVMKRAKYFIVNNELPASVFDLPS
                     ETLRKQIILGDKPKPKSQPDTQLKLFSFGDNVKPLRLTTNLAMLD"
     gene            3134..3913
                     /locus_tag="PKJ43_02000"
     CDS             3134..3913
                     /locus_tag="PKJ43_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HNZ71362.1"
                     /translation="MIYLKENMRYLRKQKKMTQGDLADKLDIKRSRIGSYEEGRGVPK
                     LSVIKQMADLFEVSVDTLLAVDLSSEEPTDNSQQPIASQPDLKLLSIVVTPENNERIA
                     IVPVKASAGYLTGRADQGYMEQLPNFSMPVSELNKGKTYRVFQIKGDSMLPVPSGSYI
                     FCDYVESVSGIREGKPYVLITADEGIVYKRVYPQNENLLLLKSDNPEYEPYTVEISSL
                     FEIWKAIGFLTFELPEPDAMNVQKLGSLVAQLQQEVSKLQG"
     gene            4009..>4372
                     /locus_tag="PKJ43_02005"
     CDS             4009..>4372
                     /locus_tag="PKJ43_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71363.1"
                     /translation="MNKTAMLLCQCRDVSENQFHKESTRQLLSGFDADVFELDDLCAL
                     AVNERSFFKEIEELYTDKIIMACYPRVVRNLLLQNEITLSNYDVINHHEVSRETQLAQ
                     KLSDRGISPGKARFSEVKT"
BASE COUNT         1127 a          914 c          993 g         1338 t
ORIGIN      
        1 gcttaggtcg taaggaaaag tattccattt tttccaaaat cccttttcaa gaatgtaggc
       61 atcttcaaag tctgttttgt agctgcttgg agggagcagc ggttcccatt tctgttcaag
      121 gtaataatca gctaaatgtc tttcgttgaa attcattacc caatcgggaa aattctccat
      181 gtaatacgac gaagagatca ttttcccgtt tagaggatcg agccaaaagg ctccgtcggc
      241 ggcatgaccg gcactaagta cggctgcaac agggtttaac gcaaccgagt acactttcga
      301 cctgaaattg gagatttctt tcatggcatc tcccaatgtg tacattttta gcatattggc
      361 cgacatattc ccgttttttg aatcgcttcc cagcgtgagg taaaaatcgt cgtttattgc
      421 atcaatttct ttctttgtaa gatgtttata ccatttgtcg cctactatgc catgttcggc
      481 agggtaagag ccggtatata ttgtgggtat tactgtcgat gtgcgtatgt tatgaatgtc
      541 gagtcttgca ttgagagagt acgcgccgtt ttcgaacagc cgtttaaaac cgccttcctg
      601 aaaggtatcc caatatcggg tcacatagtc tgaccttaaa tggtctatgg ttatgccaac
      661 caccaacttg ggggttttag cacttttcag ttggtttgtt tgggcttgta gcccgagcgt
      721 taaagcaata atcagtatac ttaaaaaaaa tctccccatc ttctgtaatt attattcttt
      781 ccaaatgtat aaaattgggt gaggtataaa aatggttcga gcgaaaagag tgcttgtcaa
      841 ccccaaaatt aaacctgtat ttgatacgag cataatttct atcgttcgtc ttgtctagcg
      901 ctttttttcg gtaaggtatt tccaaaatct cttgggcata tgctgacgct gcagttttgg
      961 gttcagccgt tcacgaatgg tggtgctttt gcaatatcca atccatgctt gctggtaaaa
     1021 accctcctct tcctgtagaa gattggccgg cattgcacct gttgacctgc tgaaggtttt
     1081 gtcggcaagg gttatttctt cggatgtttt cttgttgtag taaaaaccat aatcgcgttt
     1141 tagatcgtaa actacccaat tctggtctgc aaatcgattt tgtaattgtt tgattgttaa
     1201 aggaaggaca tcatactccg gactaatggc tgagaaataa attccatcga gggtgcattg
     1261 aaacctgacg aattgtagca cacgcatagc ctctcgcatt actcgccggg cagccttcct
     1321 gaccgttaga atattcgcat cgccaaaatt cccttcaacc gaaaggggaa gtataaatgc
     1381 ctgtctgata tattccaaaa gagccatctc gattcctttt tctcccgaga gccacgccga
     1441 ataaggtatt tgattaagtt cgggcttaag tttttgctgt aatcctttcc atacccgttc
     1501 ggcatggtca aaatcggttg ttatttcttc ctttgaagta aacatggtat gctgaaaatg
     1561 ctcttttgct gtgatttcgg tgggctcaag cttccttttg tatacttcaa acactaccga
     1621 caagtaacct tcaaatgttc cgtcgtaaac aagcaacatg atttatgaat tatgatatct
     1681 tttattaatc gagcattgct aaattggtgg ttaagcgtag cggtttcaca ttatcgccaa
     1741 acgaaaataa tttcaactgt gtgtcgggtt gcgatttggg ttttggtttg tctccaagaa
     1801 taatctgctt tcgcagggtt tcactcggaa gatcgaacac cgatgctggt agctcattgt
     1861 tcacaataaa gtatttggcc cgcttcatca ccaccccgat tttggtcagg tgttgggtgt
     1921 tgagcgttcg gaactttcgg gcttcaataa tccgctgtgc cgattgtact cctatgcccg
     1981 gtacccgcaa tatcatctcg taaggcgcct tgttcacgtt gatgggaaat tgatgcaggt
     2041 ttcgcaacgc ccacgccaat tttgggtcaa tttcaaggtc gaggaacgga tgctcgtcac
     2101 tcacaatttc gttggctttg aacttgtaaa aacgcatgag ccagtcgctt tggtaaagac
     2161 ggttttcacg caccagtggc ggcgttttga tggctggtag ccgctggtcg tattcgttca
     2221 caggcaggta accggaatag tacacccgct ttaaccgttg tccttgataa aggtccgatg
     2281 ccagcgacaa tatttgtcgg tcgctttcgg gagtagcgcc tacaataagt tgagtgcttt
     2341 gtccggcagg ggcaaacgtt ggggcatggc gaaggtgctt tcgttcttcc ttttgggcaa
     2401 taatgttctc tttaataaaa ttcatgggcc gataaatgtc gacatagttc ttttcgggtg
     2461 ctaaccgttt gagttccggg tttgaaggga tttcgatgtt cacactaagc ctgtcgaccc
     2521 aaatcccagc ctgatgaatc aagtcggaac tggctcccgg aatggctttc atgtgaatgt
     2581 agccgttgta acgctcttca atccggagct ttttggcaac cagcatcatt cgttccatgg
     2641 tgtagtcggg gcttttaata acgccagagc taaggaacag cccttcgatg taattccggc
     2701 gataaaaacc gatagttaaa tcaaccagtt cttcaacagt gagtgtggct cgcgggcggt
     2761 cgttggtgcg gcgattgatg caataggcgc agtcgtatat gcaattgtta gtcataagca
     2821 ctttcagaag gcttatgcag cgcccgtctt cagtaaagct atggcagatt ccgcagttca
     2881 ccgcgttgcc aatgcctccc tgtgtattgg cccgattatt cccgctcgac gcacacgaca
     2941 cgtcgaattt tgccgcgtcg gctaatatat ttagtttttt ctggacaata tcgttcatgg
     3001 ctaatgaaaa aagagtaact ttacaacaaa aatactaata aaattagtaa attatcattg
     3061 gcagataaga aagttttaaa cattaatttt ttgatgacta tttgttaacg gttttttcct
     3121 gaatactaaa gatatgattt acctgaaaga aaatatgcgc tacctgcgta agcaaaaaaa
     3181 gatgacgcag ggcgacctgg ccgataaact ggacattaaa cggtcgcgga ttggttcgta
     3241 cgaagagggc aggggtgtgc ccaaactttc ggtaatcaag cagatggctg atctgttcga
     3301 agtaagtgtg gatactcttt tagcagttga tctttcatcg gaagagccaa cagacaacag
     3361 ccaacagcca atagcttcac aacccgattt aaagttgctg agcattgtag ttactcccga
     3421 aaataacgaa cgaattgcca ttgttcctgt aaaagcatcg gcaggttatc tgaccggtcg
     3481 cgccgatcag ggatacatgg aacaacttcc caacttctcg atgccggttt ccgagttgaa
     3541 taaaggcaaa acctatcggg tctttcaaat aaagggcgac agcatgttgc cggttccttc
     3601 cggttcgtac attttttgcg attatgtcga aagtgtttcg ggcattcgcg aaggtaaacc
     3661 ttatgttttg attaccgccg acgaaggaat tgtatacaag cgagtttatc ctcagaatga
     3721 aaacctgctg ttgctaaaat ccgacaatcc cgagtatgag ccttatacgg tggagatatc
     3781 atctctgttt gaaatttgga aagcgatcgg ttttttaacc tttgaactcc ccgagcctga
     3841 tgcgatgaat gttcaaaagt taggttcgtt agtggcacag ttgcaacaag aagtgtcaaa
     3901 gttgcagggg taaactgtca gggtgttaat gctgtgttat tctccgtttt ttgtttcacc
     3961 ctttcctttt tttaggttat gttcgtcgta tagttttgtt agttagccat gaataaaaca
     4021 gcaatgcttc tttgccaatg ccgggatgtg tctgaaaatc agtttcacaa agagtcgaca
     4081 cggcaactgt tgtccgggtt tgatgctgat gtttttgagc tcgatgatct ttgtgccctg
     4141 gccgtgaacg agcgatcttt ttttaaagag atcgaagaat tgtacacaga taaaattatt
     4201 atggcctgct atccgcgggt tgtcaggaat ttgcttctgc aaaatgaaat aaccttgagc
     4261 aattatgatg ttataaatca tcatgaagtt agccgcgaaa cacaactcgc gcaaaagctt
     4321 tccgatcgtg gaatctctcc gggaaaggca cgtttttctg aagttaaaac tt
//
LOCUS       DAOPUW010000072         4361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_20267_length_4361_cov_4.224570, whole genome shotgun sequence.
ACCESSION   DAOPUW010000072 DAOPUW010000000
VERSION     DAOPUW010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20267_length_4361_cov_4.224570"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="PKJ43_02010"
     CDS             <1..1121
                     /locus_tag="PKJ43_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ71364.1"
                     /translation="DGGKGINEKDIPLLFARYSILSGESLYENSTGIGLSLSNEIVKM
                     HGGEIRVSSGPGNGSLFTIVLKIGNAHLKGQTNIHFVDEHEGYTATYNLLKDNFSEIE
                     PELGEKLANENGNHQHTLLIIEDNQQILSYLSEALSVDFSVILATDGKQGLELAVQHQ
                     PDLIVSDVMMPVIDGIEMTRQLKEDFSTCHIPVVLLTAKSSVDDQILGIESGAEAYVL
                     KPFNMGVLRSIINNMLEQRKLILRKYRDNKEISPSDLKITSRDKEFIDKLVKYIEENY
                     SDPELSITKLVDLSCVSRTVFYNKVKSLTGLSPIELMRQIKLKIAAQMLLNGYNVNEA
                     AINIGFNDTRYFSKQFKELFGESPSTYKKEKTTDGDYN"
     gene            1394..1774
                     /gene="rpsL"
                     /locus_tag="PKJ43_02015"
     CDS             1394..1774
                     /gene="rpsL"
                     /locus_tag="PKJ43_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007070674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNZ71365.1"
                     /translation="MPTIQQLVRKGRQSNIDKSKSPALDSCPQRRGVCVRVYTTTPKK
                     PNSAMRKVARVRLTNLKEVNAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGAL
                     DTAGVEGRTQRRSKYGAKRPKVKK"
     gene            1895..2371
                     /gene="rpsG"
                     /locus_tag="PKJ43_02020"
     CDS             1895..2371
                     /gene="rpsG"
                     /locus_tag="PKJ43_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNZ71366.1"
                     /translation="MRKSKPKKRIILPDPKFNDVLVTRFVNDLMVDGKKTVAYSVFYD
                     AMDLVSEKLKNEELSPLEIWKKALENITPQVEVKSRRVGGANFQVPMEIRPERKISIS
                     IKNMILFARKRAGRSMSEKLAAEIQAAFNEEGGAYKRKEEIHRMADANRAFAHFRF"
     gene            2435..>4361
                     /gene="fusA"
                     /locus_tag="PKJ43_02025"
     CDS             2435..>4361
                     /gene="fusA"
                     /locus_tag="PKJ43_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016779063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNZ71367.1"
                     /translation="MAKRDLQYTRNIGIMAHIDAGKTTTSERILYYTGLTHRIGEVHD
                     GAATMDWMEQEQERGITITSAATTTQWKYNDVEYKINLIDTPGHVDFTVEVERSLRVL
                     DGAVALFCAVGGVEAQSETVWRQAEKYGVPRIAFVNKMDRSGADFYNVLKEIKEKLGS
                     NPMPLQLPIGSEETFKGVVDLVQMNAIVWHETGDNGTTLTIEEIPSDMVEQANEWREK
                     LVEAVAEQDEVLMERFFEDPDSITPEDMIPVIRKATIAGTIIPMLCGSSFKNKGVQRL
                     LDAICMYLASPLDKGELKGHDIDDYDTIVTRKADADEPFVGLAFKIATDPYVGRLTFV
                     RVYSGKIEGGGTYYNPRNRKKERISRIYQMHSNKQQPRDVIEAGDICAVVGLKDVRTG
                     DTLCDIDKPLVLESMSFPEPVIGIAVEPKTQKDLDKLGMGLAKLSEEDPTFVVNTDQE
                     TGQTVIRGMGELHLEIIIDRLRREFKVECNQGKPQVAYKEAIKGAVELREVFKKQTGG
                     RGKFADIIVKVEAAENGKEGLEFVDAVKGGNIPREYVPSVEKGFKAAMSNGPLAGFPI
                     EALKVTLLDGSFHPVDSDQLSFEICAKQAFKSATSKAQPTLLEPIMKLEITTPEEYMG
                     DIIGDLNRRRGQVEGMES"
BASE COUNT         1395 a          845 c          959 g         1162 t
ORIGIN      
        1 ccgacggggg aaaggggatc aacgaaaaag atattccatt gttgtttgcg cgatattcaa
       61 ttctttcagg cgaatcgctt tatgaaaata gtacagggat tggtctaagc ctttcgaatg
      121 aaatcgtaaa aatgcatggc ggcgaaatcc gtgttagcag cggacctggc aacggaagcc
      181 tttttaccat tgtgttgaaa ataggcaacg cccaccttaa agggcaaaca aacattcact
      241 ttgtcgatga gcacgaaggc tatactgcaa catacaactt attaaaagac aatttctcgg
      301 aaattgaacc agaactaggc gaaaagcttg ccaacgaaaa cggcaaccac caacatactt
      361 tattaataat tgaagataac cagcaaattc tcagctatct cagcgaagca ttatctgtcg
      421 acttttctgt gattctggca actgatggaa aacaaggcct tgagttggct gtccagcatc
      481 agcctgatct aattgtgtct gatgtgatga tgccagtaat cgatggaatt gaaatgacca
      541 ggcagctcaa agaagatttc agcacctgtc atattcccgt ggtattactt actgcaaaat
      601 caagtgtcga tgatcagatt ttaggaattg aatcgggagc cgaagcatat gtgctcaaac
      661 ctttcaatat gggggttctc cggtcaataa ttaataacat gcttgagcaa cggaaactca
      721 tcttgcgaaa gtaccgtgac aataaagaaa tttcgccttc cgatttgaaa ataacttccc
      781 gtgacaaaga gtttatcgac aaattggtaa aatacatcga agaaaactat tctgacccag
      841 aattatcaat tacaaaactc gtcgatttat cgtgtgttag ccgcacggtg ttttataata
      901 aggttaagag tctcacgggc ttgagcccga tagagcttat gcggcagatc aaattgaaaa
      961 tagccgctca aatgttgctt aatgggtata atgtgaatga agccgccata aatattggct
     1021 tcaacgatac ccggtatttc agcaagcaat tcaaagagct ctttggcgaa tcaccaagca
     1081 catataaaaa agaaaaaacc accgacggag attacaatta attaacctct ggtaaaggaa
     1141 aaatcagcct agatattcaa taggtaatgg caaaaagaag aaattgctta ctccttgcca
     1201 cccttcagac aattacagat tttttttttg atggaaaaag cgaaaaaaaa gaaatcatct
     1261 atttgattgt tgctggaaat tagtactttt gccctccgtt tcgtgaagga ttatgcgcat
     1321 tttgcaacat aagtgactga taatcctgca agaagcatca ttaagaattt ataattaata
     1381 attttaacgt ataatgccta caattcaaca gttagttaga aaagggagac aatcaaacat
     1441 tgataaaagc aagtctcctg cacttgattc ctgtccgcag cgtagggggg tatgtgtacg
     1501 tgtttatact accactccta agaaaccaaa ctcagctatg cgcaaagtag cgagggtgag
     1561 gttaactaat ctgaaagagg tgaatgcgta tataccgggt gaaggtcata atttacagga
     1621 acactcaatc gtactcgtac gtggaggccg tgtaaaagac ttaccaggtg tgcgttacca
     1681 catcgttcgc ggagcattgg atacagctgg tgttgaagga cgcacccagc gccgctctaa
     1741 atacggagca aaacgtccta aagtaaaaaa ataaatgttg aaatggttat ttagagttgt
     1801 ggtttggttg agtaagggac tctattctat ttttaggatg aactgaagac tgacactcag
     1861 gcaaccgatt tgaataacac aaactttaaa aaaaatgagg aagtcaaaac caaagaagcg
     1921 gatcattttg cccgatccca agtttaacga tgtattagtt actcgtttcg tgaacgacct
     1981 tatggtcgat ggcaagaaaa cagttgccta ctcagtattc tacgatgcaa tggatttggt
     2041 gagcgagaaa ctcaagaacg aagaactctc acccctcgaa atctggaaga aagcattaga
     2101 aaacatcact cctcaagtcg aggttaaaag ccgccgcgta ggtggagcaa actttcaggt
     2161 tcctatggaa atccgtcccg aaaggaaaat ttcaatttct attaaaaata tgatcctttt
     2221 tgcacgcaaa cgtgcaggtc gatcgatgag tgaaaaactt gctgccgaaa ttcaggctgc
     2281 attcaacgaa gaaggtggag catacaaacg gaaggaagaa attcaccgta tggcagatgc
     2341 taaccgtgct ttcgctcatt tccgttttta gtattattta gttggtttag ttggtttttt
     2401 atagttgatt ttttaaataa gattggtttt tataatggct aaaagagatt tacaatatac
     2461 gcgtaacatc ggtatcatgg cgcatatcga cgccggtaag actacaactt cagaacgaat
     2521 tctgtattat accggtctaa cccaccgtat tggcgaggtt cacgatggcg ctgcaaccat
     2581 ggactggatg gagcaagagc aggagcgtgg tattaccatt acttctgcgg ctacaaccac
     2641 tcaatggaaa tacaatgatg ttgagtataa aattaacctg atagataccc cgggacacgt
     2701 tgacttcact gtagaagttg aacgttctct tcgcgtatta gatggcgccg ttgcactttt
     2761 ttgtgcagta ggtggggttg aagctcagtc tgaaactgta tggcgtcagg ctgaaaaata
     2821 tggtgtgcca cgtatcgcgt tcgttaataa aatggaccgt tctggtgccg acttttataa
     2881 cgttttaaaa gagataaaag aaaaacttgg ttctaaccca atgcctcttc agcttccaat
     2941 tggtagtgaa gaaacattta agggagttgt tgacttagtt caaatgaatg ccattgtttg
     3001 gcatgaaaca ggcgacaacg gaacaacact caccattgaa gaaattcctt cagatatggt
     3061 tgaacaggca aacgaatggc gcgaaaaatt agttgaagct gttgccgaac aagatgaagt
     3121 tttaatggaa cgtttcttcg aggatcccga ttctattact ccagaagaca tgattcctgt
     3181 tattcgcaag gcaaccattg ctggaactat tatcccaatg ctttgcggtt cttcatttaa
     3241 aaacaaaggt gttcagcgtc ttctcgatgc aatatgtatg tatttagcta gtccgctcga
     3301 taaaggcgaa cttaaaggac acgatatcga cgactacgat acaattgtaa cccgtaaagc
     3361 cgatgctgat gaaccatttg taggcttggc atttaaaatt gctactgatc catacgttgg
     3421 acgtttaact ttcgtgcgcg tatattcagg aaaaattgaa ggtggtggca catattacaa
     3481 cccgcgtaac cggaaaaaag aacgcatctc tcgtatttac cagatgcatt caaataagca
     3541 acagcctcgt gatgttatcg aagctggaga tatttgtgca gtagtaggtt tgaaagatgt
     3601 acgcaccgga gatactctct gtgatattga taaaccattg gttcttgaat caatgtcgtt
     3661 cccggaacca gtaattggta ttgccgtaga accaaaaaca cagaaagacc tcgataaact
     3721 cggaatggga cttgctaagc tttctgaaga agatccaaca tttgttgtga ataccgatca
     3781 ggaaacaggt cagacagtga ttcgcggtat gggtgaactt cacctcgaaa tcattatcga
     3841 tcgtttacgt cgcgaattca aagttgaatg taaccaaggt aaacctcagg ttgcatacaa
     3901 agaagctatt aaaggtgccg ttgaacttcg cgaagtattt aagaagcaaa ccggggggcg
     3961 tggtaaattt gccgatatca tcgttaaagt agaagctgca gaaaacggta aagaaggact
     4021 tgaatttgtc gatgcagtaa aaggtggaaa cattccacgc gaatatgtac cttctgttga
     4081 aaaagggttt aaagctgcaa tgtcgaacgg gcctctggcc ggtttcccaa ttgaagcgtt
     4141 gaaagttacc ctgctcgacg gttctttcca cccagttgac tctgaccagt tgtcgttcga
     4201 gatttgtgca aaacaagcct tcaaatcagc aacttcaaaa gctcagccaa cactactcga
     4261 accaattatg aagctcgaaa tcactactcc agaagagtac atgggagaca taatcggtga
     4321 cttaaaccgc cgtcgtggtc aggtagaagg aatggaatcg a
//
LOCUS       DAOPUW010000073         4317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_20522_length_4317_cov_3.551619, whole genome shotgun sequence.
ACCESSION   DAOPUW010000073 DAOPUW010000000
VERSION     DAOPUW010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20522_length_4317_cov_3.551619"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /gene="fabD"
                     /locus_tag="PKJ43_02030"
     CDS             <1..832
                     /gene="fabD"
                     /locus_tag="PKJ43_02030"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNZ71368.1"
                     /translation="DNSPVARELFDKANEILGFEITKIMFEGTADELKQTKVTQPAIF
                     LHSVILSKVLGDKFQPEMTAGHSLGEFSALVTAGALSFEDGLRLVSKRAMAMQKACEK
                     EPSTMAAVLGLDDEVVEQICKETDGIVVPANYNCPGQLVISGSIDAVNAACEQMKAKG
                     AKRALVLPVGGAFHSPLMEPAREELAAAIEATSINTPVCPVYQNVDAKPQTDPAVIKK
                     NLIAQLTGPVSWTQTVKNMIADGANSFTEIGPGKVLQGLVNKVDNSVATDGYDKLPEE
                     "
     gene            906..1808
                     /locus_tag="PKJ43_02035"
     CDS             906..1808
                     /locus_tag="PKJ43_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNZ71369.1"
                     /translation="MKKLSFKKIDAFATAKSTGNPAGFIWLNSLNEIKEETRQQIARE
                     LKGFVSEVGYAAQVGPNEIDLCYFSAEREVEFCGHATIAILYDLVCTREEFGQMPELI
                     INTKRGSLHVENRLKTEDSVFIMSPEPIYYEQVPASDKIATNLGIDASKIANHYPITI
                     INAGLSTLLVPIEDLETMLSINPELTSLKEFCVKSDIDIIEVFTPDVSGSQSHYRTRV
                     FAPRFGYLEDPATGSGNSAFGYYLLKYNLWPAETIVIEQNGEKENFNIVKLQKKTDSN
                     GTVRVWFGGKAITRIEGTYCVHND"
     gene            2074..3039
                     /locus_tag="PKJ43_02040"
     CDS             2074..3039
                     /locus_tag="PKJ43_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71370.1"
                     /translation="MRLKLILLLIIIALFVNANAQDKIITLKNDTIDCSILEINPKQL
                     IFEIDSKGIKSKGEINRSEISGYFLSANSRATFENANTKSENKQQRKPSKFFSAFQFG
                     LSGGYGSLLGSSKAAQNILISKGFGPAEVREYYDDFRGGLLIDACAHYLFFNVEGGSV
                     GCGLNYSLMTSSQSVSGYILEDIEQDGVLDWYFATYSEKYYINFYGLSIYAKKKVGPI
                     KLYLEDALGFVSYRNELKYLSGPLLLTSASLGTKSKLGIEIPVTKNLLVDINSSLFFS
                     QLSWIKRNNGYNSQKMQLDSEYKENLSRFEVALGLKYFIPKKAKQ"
     gene            3036..>4317
                     /locus_tag="PKJ43_02045"
     CDS             3036..>4317
                     /locus_tag="PKJ43_02045"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71371.1"
                     /translation="MKTYILLALICLLFTACSNDFLHSETQEIIQGQDSIYVSKDDTC
                     YNVELLINTDGANYKSFKVIRFPTELIIPEKEGKVENGKIALKIYTNSALEYMIKNNK
                     RGGVLVVEIEGVGMYSFPVFYGRDVSLTGALTFYTDSIVFDSGNTKEFGFKNESFEAV
                     DWQIVEMPSWLSVTKNQGRVEINGVETLTFMLAEGTNAFGLTTGRVVIETINPTKQYV
                     FNVTYNVSVQTYPGHIDEIEGEVVDADFDFESNRLFILTQKPNRLLIFNANDNVFSHI
                     DLEKVPTCMAFLPGYHSALIGNSVAIVSKVDVEKKLIEKDYLLDAVPFDIAVGAGDLC
                     YICPTIDQWVDLTYFNFKTGEISKNKIWVQLYESSYLTYLDAIDKLVATSIHITNGPY
                     VFDIVHDTVIAQNIYLNKEIDSRLWFTRDCSFAFG"
BASE COUNT         1411 a          762 c          862 g         1282 t
ORIGIN      
        1 tgataattct cccgttgccc gcgagctttt tgacaaagcc aatgaaattc ttggcttcga
       61 aatcacaaaa ataatgtttg aaggtactgc cgacgaactg aaacaaacca aagtcacaca
      121 acctgcaatt ttcctgcatt ctgttatctt atcgaaagta ttgggcgata aatttcaacc
      181 cgagatgact gccggtcact cacttggaga attttcagca ctggtaaccg caggagccct
      241 ttcgttcgaa gatgggttaa gattagtgtc gaaacgtgca atggctatgc aaaaagcctg
      301 tgaaaaagaa ccttcgacca tggcagctgt tcttggcctc gacgatgaag tagttgaaca
      361 aatttgcaaa gaaaccgatg ggattgtggt tccggccaac tataactgcc ccggacaact
      421 cgttatctcg gggagcatcg acgccgtaaa tgcggcttgc gaacaaatga aagcaaaagg
      481 tgcaaaaagg gctttagttc tccctgttgg tggtgctttt cactctccgc taatggaacc
      541 agctcgtgaa gaactggcgg cagctattga agcaacttca atcaataccc cggtttgccc
      601 tgtctaccaa aatgtggatg caaaacctca aaccgaccct gcagtaatta agaagaacct
      661 gattgcccaa cttaccggcc cggtaagctg gacacaaaca gtgaagaaca tgattgccga
      721 tggcgccaat tcgtttaccg aaattggacc cgggaaggta ttacaaggct tggtcaacaa
      781 agttgataat tctgtggcta ccgacggata cgataaatta cctgaagaat aaacacttat
      841 atcaaatatc gaaatcccgg ttttagcaaa tcgggatttt tttgttttat tcaattttta
      901 caaccatgaa gaaactttca ttcaaaaaga tcgatgcctt tgctaccgca aaatcaaccg
      961 gaaatcctgc cggttttatc tggctcaact cattgaacga aatcaaggaa gaaaccaggc
     1021 aacaaattgc ccgtgagtta aaaggctttg tcagcgaagt aggatatgca gctcaggttg
     1081 gccccaatga aatcgacctt tgctactttt ctgcagaacg tgaagtagaa ttttgcggcc
     1141 atgcgaccat tgccattctt tacgatttgg tgtgtacacg agaagaattt ggccaaatgc
     1201 cagaacttat aatcaataca aaaagaggca gtttacacgt agaaaacaga ttaaaaaccg
     1261 aagattcagt tttcatcatg tcgccagaac caatttatta tgagcaagtt ccagcaagtg
     1321 acaaaatcgc cacaaactta ggcatcgatg catcgaaaat tgcgaaccac taccctataa
     1381 cgattattaa cgccggacta agcaccttgc ttgttcccat cgaagacctg gaaacaatgc
     1441 ttagtatcaa tcctgaatta acctctttaa aagagttttg tgtaaagtca gatatcgata
     1501 ttattgaggt attcacgccc gatgtatctg gaagccaaag ccattaccgc acccgggtct
     1561 ttgccccaag gtttggttat ctcgaagatc cggctacagg ctcgggtaat tctgcatttg
     1621 ggtactatct tttgaaatat aatttatggc cagctgaaac cattgtgata gaacaaaatg
     1681 gcgaaaaaga aaacttcaat atcgtaaaac tacagaaaaa aaccgattca aacggcactg
     1741 tcagagtttg gtttggtgga aaagctatta cacgtataga aggaacgtat tgtgtacata
     1801 acgattaaat taccggaagg acaaatgatt ttatcatagc atatgagaga aggaatatct
     1861 tataatcaat ggataaattc ttcttgcatc caaataaatt caaaattttc catgacagat
     1921 attatcatat aaaaaacaaa gaaattattc ataaatcgat tagaaaaaga aatcaaatcg
     1981 tgaaagaaaa aattgtaata aaaatatagt gttacgataa ttagcaataa cgtttttcgt
     2041 tagttttgaa caataaaaaa actattattc aatatgagac tcaaattaat tcttctattg
     2101 attattattg cgctatttgt taatgcaaat gctcaagata agattatcac attaaagaac
     2161 gacaccattg attgttctat cctggaaatt aatccgaagc agttaatttt tgaaatagac
     2221 tccaaaggga taaaaagcaa gggtgaaatt aaccgatctg aaataagtgg atattttttg
     2281 tctgctaatt caagggcaac attcgaaaat gccaatacaa aatcagaaaa caaacaacaa
     2341 cgtaaaccct ctaaattctt cagcgctttt caatttggat taagtggtgg ctatggatca
     2401 ttgttgggaa gttcaaaagc agcccagaat attttaattt caaaggggtt tggtccggct
     2461 gaggtccgtg aatattatga tgattttcgg ggaggattgc taattgatgc atgcgctcat
     2521 tatttgtttt tcaatgtgga gggcggttca gttggatgtg ggctcaatta ttctttaatg
     2581 accagcagtc agtctgtttc gggatatata cttgaagata tagaacaaga tggcgtgttg
     2641 gattggtatt ttgcaactta ttctgaaaaa tattatatca atttttacgg cttgtcgatt
     2701 tatgctaaga aaaaagtagg tcccattaag ctttatttgg aagatgcatt gggatttgtt
     2761 tcttaccgca acgaattaaa atacttgtcg gggcctttgt tgttgacatc tgcaagctta
     2821 ggtacaaaat caaaactcgg aatagaaata cctgttacga agaatttatt ggtcgatatt
     2881 aattcgtcat tgttcttttc gcaactgagc tggattaaac gaaacaatgg gtataattca
     2941 caaaaaatgc aacttgattc agaatataag gaaaaccttt ccagatttga ggttgccctg
     3001 ggcttaaaat atttcattcc taaaaaagct aagcaatgaa aacatatatc ttattggcac
     3061 tgatttgctt attgtttacg gcatgttcga atgatttctt acactccgaa acgcaagaaa
     3121 tcatacaagg acaagactcc atctatgttt cgaaagacga tacttgttat aatgttgaat
     3181 tattaattaa tacagatgga gctaattata agtcattcaa agttatccgt ttcccaaccg
     3241 aacttattat ccccgaaaaa gaaggaaaag ttgaaaatgg aaagatcgca cttaaaattt
     3301 ataccaatag tgcgcttgaa tatatgatta aaaacaataa acgtggaggt gttctagttg
     3361 ttgaaataga aggtgttgga atgtattctt ttcctgtgtt ttatggcagg gatgtatctt
     3421 taacaggagc tttaacattt tataccgatt caattgtttt tgattcaggg aatacaaagg
     3481 agtttgggtt caaaaacgaa tcgtttgaag ctgttgactg gcaaattgtt gaaatgccaa
     3541 gttggttatc agttacaaag aatcaaggac gggttgaaat caacggtgtt gaaacactta
     3601 cttttatgtt ggctgaggga acaaatgctt ttgggctgac tactggaaga gttgttattg
     3661 aaacaatcaa tccgaccaag caatatgtct ttaatgtaac ttataatgtt tccgttcaga
     3721 cctatccagg tcacatcgac gagattgaag gtgaggttgt tgacgccgat tttgacttcg
     3781 agagtaatcg attatttata ttgacacaaa aaccaaacag attgctgatt tttaatgcta
     3841 acgataacgt ttttagtcat atcgatttgg aaaaagtacc cacttgtatg gcttttttgc
     3901 ccggatacca ttcggcacta attggaaatt cagttgctat tgttagtaaa gttgatgttg
     3961 aaaaaaagct gattgaaaaa gactatctgc ttgatgctgt tccgtttgat atagcggttg
     4021 gagcaggtga tttgtgctat atatgtccaa caattgatca atgggtcgat ctcacgtatt
     4081 ttaactttaa gactggcgag atttcaaaaa acaaaatttg ggtgcagtta tacgaaagct
     4141 cgtatttaac ttatctcgat gctatcgata agcttgttgc tacaagtatt catatcacca
     4201 atggacctta tgtttttgac attgtacatg atacggtaat tgctcaaaac atatatttga
     4261 ataaagaaat tgacagcaga ctttggttta ctcgtgactg tagctttgct ttcggca
//
LOCUS       DAOPUW010000074         4305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_20589_length_4305_cov_3.808471, whole genome shotgun sequence.
ACCESSION   DAOPUW010000074 DAOPUW010000000
VERSION     DAOPUW010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20589_length_4305_cov_3.808471"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PKJ43_02050"
     CDS             complement(<1..751)
                     /locus_tag="PKJ43_02050"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNZ71372.1"
                     /translation="MQFELSKEYIDRVEQLIEDKKDQSLKDILYDLHPADIAELLDAL
                     SEDDGKYLYLLLEGDLASDVLVEIPEDDRKRFLEEMPAEVIARQYIEYMDTDDAADVL
                     GELSDEKQEEIISFINDEEHADEITELLSYDEDTAGGIMAAELVAVNEKWSVQTCLKE
                     IGRQAEDIDEIYYVYVINNERKLKGILSLKKLIQYPTSTLISNIYFKDVISIHIDASR
                     EEVARQMEKYDLVALPVVDSEGHLKGRITIDD"
     gene            complement(741..1535)
                     /gene="rsmA"
                     /locus_tag="PKJ43_02055"
     CDS             complement(741..1535)
                     /gene="rsmA"
                     /locus_tag="PKJ43_02055"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNZ71373.1"
                     /translation="MVVVRAKKNLGQHFLTDKSIALKIVESLVSDVSNVLEIGPGMGV
                     LTEYLFQSDRYKTIALDIDRESVEYLKQRFPSYAEQVLYGDFLRSDLNSMFSGERVAI
                     IGNFPYNISTQILFRVLDFKDIVPEVVGMFQKEVAQRIAAPPGSKVYGITSVLLQAYY
                     SIDYLFTVDEHVFSPPPKVKSAVIRLTRNNVQKLDCDEKLFARVVKAGFNQRRKMLRN
                     SLKSLIQCDGFLHPFLEKRPEQLSLAEFVELTQSISSFLTSKNNAI"
     gene            1654..2676
                     /locus_tag="PKJ43_02060"
     CDS             1654..2676
                     /locus_tag="PKJ43_02060"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNZ71374.1"
                     /translation="MKKKILNILKYVLFTSLGVGMFWLVYRDQDFTHIFNVLKNEVNY
                     SWVALSLFLGLLSHISRTLRWKIALEPLGEKPKTSNTFITIMASYFMNLLLPRMGEFV
                     RCGLLSKYEKIPFTKLFGTVVTERIVDVIMLFVLLLLMAILEMDKLIAFSDHNPGVVE
                     KIQSMLSSPILWIAIILILGSVIFYVKISRKKGKENKITELLHEFGAGIKSILAMKRY
                     KAYIFHTVFIWTMYFLMLYFYFFSLEFTRHLNIEAALTTFTMTSFSMVAPVQGGIGAW
                     HFMAEQSLLLYGIESADGKLFALLAHSSTNIFVLILGAVCLIILPMVNRKPTPKTAES
                     TEITES"
     gene            2701..3975
                     /locus_tag="PKJ43_02065"
     CDS             2701..3975
                     /locus_tag="PKJ43_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ71375.1"
                     /translation="MNINKIRTLNTELFIARKVFSQKENKNGISSKIVSIAVASISLG
                     LAVMIISVAILVGFKREIRDKVVGFGSHFQIVNFDSNASFETQPISIDSVMIKQFLNA
                     GHVNHIQAFGTKPGLIKTDDAIHGVIVKGVDTNFNWEFFSQNLTKGNIPSYKPDGKSN
                     EVIISEKISKLLKLNIGDPLFCFFYNQGENAPRSRKFTISGIYRTSLDEFDEMFVIAD
                     LRHIQDLNNWNSNEVTGYELTIDNFEYLTPTFEKLREITINNANENSTLRVYSIITRY
                     PMLFDWLSVLDLNVWVLLFLMVAVAGINMISGLLVIIIERTRMIGILKALGFPNISVR
                     KVFLYLSGFLSAKGLLWGNVIGIGLCMFQYFTGIIKLNPDTYYIDTVPIVMNIGYIIL
                     LNIGSLLCIVAMIILPSLFITRISPVEAIVVE"
     gene            3990..>4305
                     /locus_tag="PKJ43_02070"
     CDS             3990..>4305
                     /locus_tag="PKJ43_02070"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNZ71376.1"
                     /translation="MKSISPDIIKEALSRPLPASEAHRLFLPPARSLEMSEEIKRHVK
                     HSAVMLLLIDNDGSLSLCLTRRNSHMKHHPGQISFPGGKMDEGENDHLETALRELEEE
                     TGI"
BASE COUNT         1289 a          892 c          830 g         1294 t
ORIGIN      
        1 catcgtcgat ggttatacgg cctttaagat ggccttcaga atcgacaacg gggagagcta
       61 caaggtcgta cttttccatc tgacgagcca cttcctcgcg cgatgcatcg atatgaatac
      121 ttatgacgtc tttgaaataa atgtttgata ttaaagtaga tgttgggtat tgaattagtt
      181 ttttcaacga aagaatacct tttagctttc tctcgttatt gatcacatac acgtaatata
      241 tttcgtcgat atcttcggct tgcctcccaa tttctttcaa acaggtttga acgctccatt
      301 tttcattaac agccaccagc tcggccgcca tgatcccccc cgcagtatct tcgtcgtacg
      361 aaagcagttc agtgatctca tcagcgtgtt cttcatcgtt gataaaagat atgatttcct
      421 cttgtttttc gtcggagagc tcgcccaaaa cgtcagcagc atcatcggta tccatatact
      481 caatatactg acgggcaata acttctgcgg gcatttcttc aaggaaacgt ttgcggtcgt
      541 cttctgggat ttcgaccaaa acatcggatg ccaaatcacc ttcgagcaaa agataaagat
      601 actttccatc atcttcagaa agtgcatcca acaattcagc gatatctgcg gggtgaaggt
      661 cgtataatat atcttttagt gattgatctt ttttatcttc tatcagttgt tctacccgat
      721 cgatatattc ttttgaaagc tcaaattgca ttgtttttac ttgttaaaaa tgaagaaata
      781 gattgtgtta actctacaaa ttcagccagt gaaagctgct cgggccgttt ctctaaaaat
      841 gggtgcaaaa acccatcgca ttgaattaac gattttaaac tgttcctcaa catttttctt
      901 cgctgattga acccggcttt cacaactcgg gcaaagagtt tttcgtcaca atcgagtttt
      961 tgaacattgt tacgtgtaag gcggattact gccgacttta cttttggtgg tgggctaaac
     1021 acatgttcat ctaccgtaaa caagtaatcg atagaatagt aggcttgtaa caacacgctt
     1081 gttattccgt atactttcga cccgggaggc gctgcaatgc gctgagcaac ctctttctga
     1141 aacatcccca ctacttcggg aacaatgtcc ttgaaatcga gaacacggaa taaaatctgg
     1201 gtagaaatat tgtatgggaa attaccgatt atggcaaccc gttcaccact aaacattgaa
     1261 tttaaatcag atcgcagaaa atctccataa agtacctgtt ctgcataaga aggaaaacgc
     1321 tgctttaaat actcaaccga ttcccggtcg atatcaagtg caattgtttt ataacgatct
     1381 gattggaaaa gatattcagt taatacgccc atccccggcc cgatttccag cacattggat
     1441 acatcggata ccagactttc aacaattttg agggcaatag atttgtccgt caaaaaatgt
     1501 tgtccaaggt tcttttttgc ccttacaact accataagga tcggggtcgc tcatctctga
     1561 atttatgatt aacatttctt gttgtttctg ttcgaaacaa atgcttattt ttgtgctgca
     1621 aaagtaacaa agctaatctt aaactttcct gttttgaaaa aaaagattct taatatttta
     1681 aaatacgttc tttttacgtc gttgggagtt ggaatgtttt ggctcgtgta ccgcgatcag
     1741 gattttaccc atattttcaa tgtgctcaaa aacgaagtga actattcatg ggttgcttta
     1801 agcctttttc tagggttact tagccatatc agccgaacac tccgatggaa aatagccctt
     1861 gaaccgctgg gcgaaaaacc gaaaaccagc aacacgttta ttaccatcat ggcttcttac
     1921 tttatgaacc tgctccttcc caggatgggt gaatttgttc ggtgcggact gctttcgaaa
     1981 tatgaaaaaa taccgtttac aaagcttttt ggtacagtag taaccgaacg cattgtagat
     2041 gtaattatgc tgtttgtttt gctattgcta atggcaattc ttgagatgga caaacttatt
     2101 gccttctcag accataatcc aggtgttgta gaaaagatcc aatcaatgct ttcatcgcca
     2161 atactttgga ttgcaataat tttaatcctc ggatcggtca ttttctatgt taaaatttcc
     2221 agaaaaaaag ggaaagaaaa taaaattacc gaactgcttc acgaatttgg ggcaggaata
     2281 aaatcaattc tggccatgaa aagatataaa gcatacattt ttcatacagt cttcatttgg
     2341 acaatgtatt tcttaatgct ctatttctac ttcttcagcc ttgaattcac aagacatttg
     2401 aatattgaag cagcgctcac cacattcacc atgaccagtt ttagcatggt tgcaccggtt
     2461 cagggcggca ttggagcctg gcattttatg gccgaacaat cgctgttgct ctatggaatc
     2521 gaaagtgccg atggtaaact ttttgcttta cttgcccact caagtaccaa tatttttgtg
     2581 ctaatactag gtgcagtatg tttaattata ttgccaatgg taaaccgaaa accaaccccc
     2641 aaaacagcag aatcgactga gatcactgag tcttgagcag tgcaaaaaac aaattaagaa
     2701 ttgaatataa acaagataag gacattgaat accgaattgt tcatagcacg aaaagtattt
     2761 tcgcagaaag aaaataaaaa cgggatatcg agtaaaatcg tttcaattgc agtggcaagt
     2821 atttcgcttg gactggcagt tatgattata tcggttgcca ttctggtggg tttcaaacgt
     2881 gaaatcaggg acaaagtggt tggatttggt tcgcatttcc aaattgtcaa tttcgattcg
     2941 aatgcgtcat tcgaaaccca gcccatcagc atcgattctg tgatgataaa acagttcctc
     3001 aacgctggac atgtaaatca cattcaagct tttggcacaa aacccgggct aattaaaacc
     3061 gatgatgcca tacatggcgt tattgtaaaa ggcgttgaca ctaatttcaa ctgggaattc
     3121 ttttcacaaa acctgaccaa aggaaacata ccttcgtata aaccagacgg aaaaagcaac
     3181 gaagtaatta tttctgaaaa aatatcaaaa ctgctgaaac tcaatattgg cgatccgctt
     3241 ttttgctttt tctacaatca gggagagaat gctccacgca gccgaaaatt tacgattagt
     3301 ggcatttacc gcaccagcct cgacgagttt gatgaaatgt ttgtaattgc cgatttaagg
     3361 cacatccagg atctcaacaa ctggaatagt aatgaagtaa ctggttatga actcactatc
     3421 gacaacttcg agtacctaac ccccactttc gagaagttgc gcgaaataac catcaacaat
     3481 gcaaacgaaa attcgacatt gcgcgtatat tcaatcatta cccgttaccc aatgctattc
     3541 gactggcttt cagtgctcga cctgaatgtt tgggttttat tatttctgat ggttgcagtg
     3601 gctggaatca acatgatctc agggctacta gttataatca ttgaacgaac ccgaatgata
     3661 ggaatcctta aagccttggg gtttccgaat atcagtgtga gaaaggtttt cctttacctg
     3721 tctggcttct tatcggctaa aggattgctt tggggcaatg taattggcat cggactgtgt
     3781 atgtttcaat actttacagg aattatcaaa ttaaatcccg atacatatta tatcgacact
     3841 gttccaattg ttatgaatat cggctatatt attttgctga atattggcag tcttttatgt
     3901 attgtcgcaa tgattattct gccttcgcta tttattaccc gaatttcgcc tgttgaagct
     3961 attgtggtag aataaaaaca cagacaccct tgaaatcaat aagtcctgat attatcaaag
     4021 aagccctgtc gagacctctg cctgcaagcg aagctcacag actctttcta cccccagccc
     4081 gttctctgga aatgagcgaa gaaatcaaaa gacatgtaaa acatagtgca gtaatgcttt
     4141 tgctcatcga caacgatggc agcctttcgc tatgcctaac ccgccgaaac agccatatga
     4201 aacaccatcc gggacaaata agtttcccgg gaggaaaaat ggatgagggt gaaaacgatc
     4261 acctggagac cgcattacgc gagttagaag aggaaacagg aatag
//
LOCUS       DAOPUW010000075         4272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_20815_length_4272_cov_3.197297, whole genome shotgun sequence.
ACCESSION   DAOPUW010000075 DAOPUW010000000
VERSION     DAOPUW010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20815_length_4272_cov_3.197297"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /gene="surE"
                     /locus_tag="PKJ43_02075"
     CDS             complement(<1..605)
                     /gene="surE"
                     /locus_tag="PKJ43_02075"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNZ71377.1"
                     /translation="METRKKKILITNDDGIHAKGLAELIDIAREFGDVLVVAPHTSRS
                     GMSKAITVDLPVRLKKYEETSNFTSYSCTGTPVDCVKLAFNQLLKNNPDIIISGINHG
                     SNSSVNIHYSGTMGAVIEGCIHEVPSVGFSLCDHLADADFEPSKKYFKKIIAETLRNG
                     LPKGTCLNVNAPKGEPKGIAVRRQGHGRWVEEFERRTDPHN"
     gene            complement(764..970)
                     /locus_tag="PKJ43_02080"
     CDS             complement(764..970)
                     /locus_tag="PKJ43_02080"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNZ71378.1"
                     /translation="MVCHCKMVSIKEIKRFSTKYPNASFDELMLSTGVSTGCGRCKSL
                     AQSHFDSFRKNGKKDTQLLLNFER"
     gene            complement(993..1595)
                     /locus_tag="PKJ43_02085"
     CDS             complement(993..1595)
                     /locus_tag="PKJ43_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNZ71379.1"
                     /translation="MYLLTEAIRIENKELQHIDLHNCRINKARKIVFGQTESIDLSKI
                     INIPTNIGNERYKCRVIFHTDKTEYEIHPYVQRKIETLKVVHHNTIEYTFKNTDRSQL
                     DEAFSKRDGCDDIIIVKNGFISDSWAANIILFDGKKWVTPNTPLLRGTQREYLLKTGF
                     IEEQAIKVDDIKSFSKIRLINAMIDFDRAPELDVETQVIL"
     gene            complement(1579..2523)
                     /locus_tag="PKJ43_02090"
     CDS             complement(1579..2523)
                     /locus_tag="PKJ43_02090"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF005486.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HNZ71380.1"
                     /translation="MNKRASMADPFLFVIDFSMHNCHVLSPQEALSKNILFNFENKTN
                     YEPDQPVKKVRICSKKPIDFSRYQQSFNYVQEQIALGNSYLVNLTFPTPITLDGSLSE
                     LFFAAKSRYKILFDNQFISFSPETFVKISNGEISTYPMKGTIDATMPNAREKILSNIK
                     EMAEHATITDLLRNDLSIVSSQVEVSRYRYLEEINTLENNILQVSSEIRGKLPADYKN
                     HLGDIIFSMLPAGSVSGAPKSKTLEIIRNAEEYNRGFYTGIAGFFDGKDLNSCVLIRF
                     IENHNGELQYKSGGGITSQSIAEEEYNELISKIYVPAN"
     gene            2654..2725
                     /locus_tag="PKJ43_02095"
     tRNA            2654..2725
                     /locus_tag="PKJ43_02095"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2687..2689,aa:Arg,seq:ccg)
     gene            complement(2838..3671)
                     /locus_tag="PKJ43_02100"
     CDS             complement(2838..3671)
                     /locus_tag="PKJ43_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71381.1"
                     /translation="MKILIYALIACIVLLSCETQAPSIPSTLKENSRKLPEITKILFI
                     GNSHTNRNNIPKTIQMMAASAGDSTYSHNETQPGYTLANHCAREATLKTIDSLKWDFV
                     ILQEQGGTQAVPDFMIDTTIYQYADILIDAIKRNSSNTKIILYMTHAYQEGVLSFNDT
                     TWAKNEPLVSTYRGMQDRIRENNIAMAEWFNVEVSPAGVMWKIFTDSYPDVNLFNSDE
                     IHPLPNGSYLLACAIYSSIYKKSPVGKYIPADVKKEEGIAMQNLVYQSLFKGKPDWKT
                     Y"
     gene            4078..>4272
                     /locus_tag="PKJ43_02105"
     CDS             4078..>4272
                     /locus_tag="PKJ43_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71382.1"
                     /translation="MKKILSVIAALLIGSIVFGQKAEKVKKEVLGDQNGSEVTLFTLT
                     NKEGNVLKLTNYGARIVRIEV"
BASE COUNT         1125 a          804 c          930 g         1413 t
ORIGIN      
        1 tcattgtgcg ggtcggttct tctttcaaat tcttcaaccc aacgcccgtg cccctggcgc
       61 cttactgcaa ttcctttggg ttcgcctttg ggtgcattaa catttaggca agtgcctttg
      121 ggtaagccgt tccgcagtgt ttcggcaatt attttcttga aatatttttt tgaaggttca
      181 aagtcggcat cggccaggtg gtcgcaaagg gaaaaaccaa ccgaaggcac ttcatgaatg
      241 cagccttcga taacggctcc catagtgccc gaatagtgaa tgttcaccga cgaattcgag
      301 ccgtgattga tccccgagat aatgatgtcg gggttgtttt taagtagttg attgaacgca
      361 agtttaacac aatcgacagg ggtgccggtg caactgtacg aagtgaaatt tgacgtttct
      421 tcgtattttt ttagccttac gggtaagtcc actgtaattg ctttcgacat tccagaacgc
      481 gaggtgtgag gcgcaacaac cagtacatca ccaaattccc gggcgatatc aatcagctcg
      541 gcaagtcctt tggcatgaat tccgtcgtcg ttggtaatga gtatcttctt tttccgtgtt
      601 tccattcttg actctattta ttgttgctat atagttgttt tatttcttat tgcgagtgga
      661 ttgtcgatta ttttcctgcc tgctaatgca agcctaaaaa taggaattgt tgcggcaatc
      721 acctaatctt atcacaatta taaagggcaa tgtaatttcg atattaacgt tcaaaattga
      781 gcaaaagttg tgtgtctttt ttgccgtttt tcctgaagct atcgaaatgg gattgtgcta
      841 atgatttgca acggccacac ccggttgaaa ccccggtact cagcatcagc tcgtcgaacg
      901 atgcattggg gtatttagtt gagaatcttt ttatttcttt gatgcttacc atcttgcaat
      961 ggcaaaccat tgtgttgctt ttttccgcca tactataaaa taacttgagt ttcgacatct
     1021 aattcagggg cccgatcaaa atcaatcatg gcatttatta gccgtatttt gctgaatgat
     1081 tttatgtcgt cgacttttat ggcttgttct tcaatgaagc ctgttttgag caagtattca
     1141 cgttgggttc ctctaagtaa gggcgtattg ggtgttaccc attttttccc atcgaaaagg
     1201 atgatattgg cagcccacga atcgcttata aacccatttt taacgatgat aatatcgtcg
     1261 cagccatcgc gtttcgaaaa ggcttcgtcg agctggctac gatcggtgtt tttgaatgta
     1321 tattctatcg tgttgtggtg aacaaccttt agcgtttcaa tttttcgttg cacataggga
     1381 tgaatttcat actccgtttt gtcggtatga aaaatcaccc ggcacttgta gcgttcgttt
     1441 ccgatgttag tggggatatt tataatcttc gataaatcaa tagattccgt ttgtccgaaa
     1501 acaatcttcc gagccttgtt aattctgcaa ttatgcaggt cgatgtgctg taattcctta
     1561 ttttcaatcc ttattgcttc agttagcagg tacatatatt ttactgatta gttcgttata
     1621 ttcttcttct gcaatgcttt gcgaggtgat tccccctccg cttttgtatt gtaattctcc
     1681 gttgtgattt tcaataaatc ggattaacac gcagctgttt aagtctttac catcgaagaa
     1741 cccggctatg cccgtgtaga agcccctgtt gtattcttct gcatttctaa taatttccag
     1801 cgttttactt tttggggcac ccgagaccga acctgcgggt aacatcgaaa aaataatatc
     1861 acccaggtgg tttttgtagt ccgccgggag tttgccccta atctcggagc ttacttgcaa
     1921 aatgttattt tccaaggtgt tgatctcttc gagataacgg tagcggctta cttcaacctg
     1981 gctcgaaaca atgcttaaat cgtttcgcag caagtcggtt atggttgcat gttcggccat
     2041 ttctttgata ttcgaaagaa ttttttcgcg ggcattgggc atggtggcgt caatggttcc
     2101 tttcatcggg taagttgata tttccccgtt tgagattttg acaaatgttt cgggagaaaa
     2161 ggaaataaac tggttgtcga acaatatctt ataccgtgat ttggcggcga agaacaattc
     2221 gcttaatgag ccatcgaggg tgattggggt ggggaaggtg aggttcacca gatatgaatt
     2281 tccaagggca atttgctctt gtacatagtt gaatgactgt tggtagcgtg agaaatcgat
     2341 tggctttttt gagcaaatcc gtactttttt tactggttgg tctggttcat aattcgtttt
     2401 gttctcaaaa ttgaaaagta tattttttga gagggcttcc tgtggagaca aaacatgaca
     2461 gttgtgcatc gaaaagtcga tgacaaataa gaacgggtcc gccattgagg ctcttttgtt
     2521 catttcatgc tgtgcctgtt ttttgttcat gtggcaaaaa taggttgaaa tagagttgac
     2581 atggcaattt ttgaacaaaa agtaaggaag cgcttttaaa acaactgaag ttttttaaat
     2641 ttgtgcccgc tatggtccca tagttcaatg gatagaattt cagattccgg ttctgacgat
     2701 gtgggttcga gtcccgctgg gatcacaaaa agaccaacta tttgaatata agtagttggt
     2761 tttttatttt gaatattttt gaactgaggc tacccccacg ggtgactggt gcagaatgtg
     2821 ggaatcgaac aacgctctca ataggttttc cagtcaggct ttcccttaaa tagagattga
     2881 taaacaaggt tttgcatcgc aattccttcc tcttttttaa catcggccgg tatgtatttc
     2941 cccacaggac ttttcttgta aattgaactg taaatagcac atgcaagcag atacgaacca
     3001 ttgggcagcg gatgaatttc atcgctatta aaaagattta catcgggata ggagtccgtg
     3061 aatattttcc acatcacacc agccggagat acttccacgt taaaccattc ggccatcgca
     3121 atgttatttt cacgaatccg gtcctgcatc ccacggtaag tcgatacaag cggctcgttt
     3181 tttgcccagg tagtatcgtt aaacgataat acaccttctt gatatgcgtg tgtcatgtaa
     3241 aggattattt tggtattact gctgtttctt ttaattgcat cgataaggat atcggcatat
     3301 tggtaaattg tggtgtcgat cataaagtca ggtactgcct gtgttccacc ttgctcctga
     3361 agtataacaa aatcccattt aagcgagtca attgttttca gagtcgcttc acgggcacaa
     3421 tgatttgcaa gggtgtatcc cggttgcgtt tcattatggg aatatgtgct gtcgcctgcc
     3481 gatgcggcca tcatctggat cgttttagga atattatttc tattggtgtg gctgttacca
     3541 ataaacagta ttttggtgat ttcgggtaat ttccttgaat tttcttttag agtacttggt
     3601 atggatggtg cttgagtttc gcatgagagc aatactatac aggcaattaa agcgtaaatg
     3661 agtatcttca tatagtctca aatcaggttt atatacaata gacacctcag ttggtaaatt
     3721 cccgtacgat gaaaaataat ttttatgcaa atcaacggat accctatgct atcgcacgat
     3781 tagcgatagc gtatcatttg ggttagctgt tgttgaaaag aatattcaaa ttgatttcaa
     3841 taaatctgaa ccagtggggg tataaagttt tgcttttgaa gtgcaaagtc tggaaaaagc
     3901 aggcatttgt agaaagatca acaagccagt tcctttagat ttaaacctaa ataacaaata
     3961 cgaattttat tttcgcctta aagcttctca tcgaataaaa aatctttcaa ttgccgattt
     4021 ttcttgatag ttttggttgg tataaacaaa cagattaaca aaaccaaaag aatagaaatg
     4081 aagaaaattt tatcggttat agctgcgctt ctaattggca gcatagtctt tggtcagaaa
     4141 gccgaaaagg ttaagaaaga ggtactgggt gaccagaatg gaagtgaagt gactcttttt
     4201 accctgacta ataaggaggg gaacgtattg aaattgacca attatggtgc aagaattgtt
     4261 cggatcgaag tt
//
LOCUS       DAOPUW010000076         4267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_20859_length_4267_cov_3.707977, whole genome shotgun sequence.
ACCESSION   DAOPUW010000076 DAOPUW010000000
VERSION     DAOPUW010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20859_length_4267_cov_3.707977"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PKJ43_02110"
     CDS             <1..279
                     /locus_tag="PKJ43_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71383.1"
                     /translation="WKEYLQKRAAEVAPVFERSGIKSWTEFCVRFAHSFSQVRATVGS
                     TSNAENLNEFLLAAESIEPLPYDIVHDIVKLQYRWSDEMDIHAEPWSM"
     gene            448..2727
                     /gene="bglX"
                     /locus_tag="PKJ43_02115"
     CDS             448..2727
                     /gene="bglX"
                     /locus_tag="PKJ43_02115"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HNZ71384.1"
                     /translation="MNKLTILLLYLAGLVLPVCAQQNEMDRFITDLLGKMTLQEKIGQ
                     LNQVVPGGGAVTGSGISQGVETKIKEGKVGSMLNSPSVEYTRKAQELAVTTTKSKIPI
                     LFGLDVIHGYKTIFPIPLALSCSWDIDQIERSARIAAIEASSAGVGWTFSPMVDISRD
                     PRWGRVAEGGGEDPYLGSRIAEAMVKGYQGNDLRGETSILACVKHFALYGASEAGRDY
                     NTVDMSRLSMYQYYFPPYKAAVDAGAGSVMTSFNVIDGVPSTGNHWLHTEVLRNQWGF
                     NGFVVTDYNAILEMIPHGLGDIKTVSALALNAGVDMDMVDEGFLSTLEKSVEDGSVSV
                     ENIDKACRRILEMKYKLGLFDNPYRFINEERMKTDLLSAQHRKAAREISQRSIVLLKN
                     SDKTLPLKKEGRIAVVGPLAKSKIDMLGTWTISGDPSEAVTLVEGITNVGGNKVSVNY
                     ARGSQFTTDPMLQRNGGNVWVRVVEEKTETVSDEKMLSDALEAARKADVIVAAVGESA
                     GWSGEASSRSDITIPECQKQLLKKLKETGKPVVVVVFSGRPLDLSWEESQFSTIIQAW
                     HGGTEAGNALADVLFGDYNPSGKLSMSFPRSVGQIPVYYNHFNTGRPYNAMSKFSSKY
                     LDVVNDPLFPFGYGLSYSTFEYGNIELSNNKPVGETKIEASVVIKNTSKYAGEEVVQL
                     YISDKVAAVSRPVKELKGFKKVMLQPGESKKVTFEIGTKELKYYNNNLEYQWEPGDFV
                     VYIGTSSADNRSANLTWMK"
     gene            complement(2784..3542)
                     /locus_tag="PKJ43_02120"
     CDS             complement(2784..3542)
                     /locus_tag="PKJ43_02120"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HNZ71385.1"
                     /translation="MKKKFLLIAIISSSILLTSCDILLQTASTVMQSEQPLTTTEVAN
                     GLKEALKIGTDTATTRIGKVNGYLLDKVIKINLPPQTAEVVKYAQKVPGLDKLIDDVI
                     TQINRSAEDAAKQAAPIFKTAITQMTIADAWGILNGADTSATNYLRDKTYQQLFSLYR
                     PVMQKSLAKPIVGGVSAEKTWNELTSKWNQFANSMAGKLVQAHPITVNLDEYVTNQAL
                     RGLFIKVGDQEKEIRTNINARVTDLLKRVFGQKK"
     gene            3689..>4267
                     /locus_tag="PKJ43_02125"
     CDS             3689..>4267
                     /locus_tag="PKJ43_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNZ71386.1"
                     /translation="MRKFSVFMAAILVASLAKSGIAQNPLAQPAPLDPEIRYGVLDNG
                     LTYYIRHNQEPKERASFYIIQNVGALLEEDNQNGLAHFLEHMAFNGTKHFEGKGILNT
                     LEKHGVAFGRNINAYTSFSETVYNLSEVPTTHEGLVDTCLLVLSDWSHYLLLTDKEID
                     NERGVLTEEWRTRRNASFRMRSQWFPVLFKGSQ"
BASE COUNT         1189 a          816 c          983 g         1279 t
ORIGIN      
        1 tggaaagaat atcttcaaaa acgggctgcc gaagtagctc ctgttttcga gcgttcaggc
       61 atcaaaagct ggactgaatt ctgtgttagg tttgctcaca gcttttcgca agtgagggct
      121 actgttggtt caacctctaa tgccgaaaac cttaacgagt ttcttttggc tgccgagtct
      181 attgaaccat taccttacga tattgtacac gatatagtaa aacttcagta tcgatggtct
      241 gatgagatgg atattcatgc agaaccttgg tcaatgtgat atgagataag tatctgaaat
      301 aaagtgggat taatcataca tttctcaata ggctaattgt ctgttgagaa atgttttttt
      361 tgtcgttgat gtattcctgt atagttgttc tgtggtgttt gtttactaca aacaacctat
      421 attgcaacca aaacaaatac atcagtcatg aataaattaa caattctatt gctttatctt
      481 gcggggcttg ttttaccggt gtgcgctcag caaaacgaaa tggacaggtt cattaccgac
      541 ctgttgggaa aaatgacgtt gcaggaaaaa ataggccagc tcaatcaagt ggttcctggc
      601 ggaggtgctg ttaccggttc tggcattagc cagggtgttg aaacaaaaat aaaagaaggt
      661 aaggtgggca gcatgctcaa cagcccaagt gttgaatata cgcgcaaggc tcaggagttg
      721 gccgttacca ctacgaaatc gaaaattcca atcctttttg gtcttgatgt tattcacgga
      781 tacaaaacaa tatttccaat cccgctagct ctttcctgct cctgggatat cgaccagatt
      841 gagcgcagtg ctcgtattgc agctatcgaa gctagtagtg ctggagtagg atggacattt
      901 tcaccaatgg tcgatatctc acgcgaccca cgctggggac gtgttgccga gggtgggggc
      961 gaagatccct atttaggctc ccgaattgct gaagcgatgg ttaaaggcta ccaaggcaat
     1021 gaccttaggg gcgaaacctc tattctggct tgtgtaaagc actttgccct ttatggcgct
     1081 tctgaagctg gacgcgatta taataccgtg gatatgagca ggctgtcaat gtatcaatat
     1141 tattttcctc catacaaagc agctgtcgat gctggagctg gtagtgtaat gacttctttc
     1201 aatgttattg atggagtccc ttctacaggt aaccattggc tgcataccga agtgcttcgg
     1261 aatcagtggg gttttaatgg ttttgtggtc actgattata atgccattct tgaaatgatc
     1321 cctcatgggt tgggcgatat taaaacagtt tcagcactgg cccttaatgc cggggtcgat
     1381 atggatatgg ttgatgaagg tttcttatcg acactcgaaa agtcggttga agatggttcg
     1441 gtttcagtag aaaacatcga taaagcttgc cgtcgcattc tggaaatgaa atataaatta
     1501 ggtctcttcg ataatccata tcgttttatt aatgaagagc ggatgaagac cgatcttctt
     1561 tctgcccaac accgtaaagc tgcccgtgaa atatcacagc gctcgatagt gctcttgaaa
     1621 aatagcgaca aaacactgcc attgaagaaa gaaggaagaa ttgcagtggt tgggcctttg
     1681 gccaaaagta aaatagatat gttgggaacc tggactattt cgggcgatcc atctgaggct
     1741 gttactctgg ttgaaggaat aaccaatgtg ggcggcaaca aagttagtgt taactatgcc
     1801 cgtggttcac agtttactac cgatccgatg ttgcagagaa atggaggcaa tgtatgggtc
     1861 cgtgttgttg aagaaaaaac agaaacagtg tcggatgaaa aaatgctcag cgatgctctt
     1921 gaagctgcac gaaaagcaga tgttattgtg gctgcagttg gtgaatcagc aggttggtcg
     1981 ggcgaagctt caagccgctc tgatattaca attcctgaat gccagaaaca attgctaaag
     2041 aaactcaaag aaacaggcaa acctgtagtt gtggttgttt tcagtggtcg tccgcttgat
     2101 ctatcctggg aagaaagcca attcagtaca attattcagg cttggcacgg gggtaccgag
     2161 gccggaaatg ctttggccga tgttcttttt ggcgattata atccttcggg taagctctct
     2221 atgtcgtttc cacgaagtgt aggtcaaatt cctgtatatt ataatcattt caatactggg
     2281 cggccatata atgcgatgag caaattctct tcgaaatatc tcgatgtggt taacgatccg
     2341 cttttcccct tcggttacgg attgagttat tcaacgtttg aatatggcaa tatcgaatta
     2401 tcgaacaata agccagtggg tgaaacaaaa attgaagcct ctgtagtaat taaaaatact
     2461 agcaagtatg ctggtgaaga ggttgttcaa ttatatattt cggataaagt tgctgctgtt
     2521 tcgaggcctg taaaagaact caaagggttt aaaaaagtga tgcttcaacc aggcgaaagc
     2581 aaaaaagtga ccttcgaaat tggaacaaaa gaattaaaat attacaacaa taaccttgaa
     2641 tatcagtggg aaccaggaga ttttgttgtt tatattggca ctagttctgc cgataatcgt
     2701 tcagcgaatc ttacctggat gaagtagagt aaaaaaaacc gcagagatcg gggaaaaaat
     2761 cctttttctc tgcggtttat tttctatttt ttctgtccga atacccgttt caataaatca
     2821 gtaacccggg cattaatatt cgttcgtatt tctttttcct gatcgccaac ctttatgaac
     2881 agcccacgga gtgcctgatt cgtaacatat tcgtcgaggt ttacggttat tgggtgtgcc
     2941 tgaacgagtt ttcctgccat tgagtttgca aattggttcc atttcgatgt gagttcgttc
     3001 cacgtttttt cggccgaaac gccaccaaca atcggttttg ccagcgattt ttgcatcacc
     3061 ggacgatata ggctgaatag ttgctggtag gttttatcgc gaaggtagtt tgttgccgag
     3121 gtatcggccc cattcagtat tccccacgca tcggcaatgg tcatttgtgt gatagcggtt
     3181 ttgaatatcg gggcggcttg tttggcggca tcctctgcag agcggttaat ctgggttatc
     3241 acgtcgtcaa tcagtttgtc gagacctgga actttttgcg catattttac aacttcagcg
     3301 gtttgtggtg gcaggtttat ttttattact ttatcgagca ggtaaccatt tacttttcct
     3361 attcgtgtgg tggcggtatc tgttccaatt ttcagggctt cttttagccc gttggctact
     3421 tcggtagtgg taagtggttg ttcactttgc atgactgttg aggcagtttg aagcaaaatg
     3481 tcacacgaag tcagtaagat agatgagcta attatggcta tcaacaaaaa tttctttttc
     3541 atttttaatc ttttttaaat tgaatttaac taatgtacaa aaaatatttt acgaagtttg
     3601 taacaaaatg tataccccga acactaatta cacaactata aagttaataa tttggattaa
     3661 tcccactaag acaataaaaa gtaaagtaat gagaaaattt tccgttttta tggctgcaat
     3721 acttgtagcc tcgcttgcca aatctggtat agcacaaaac ccgttagctc aacctgctcc
     3781 cctcgatcca gaaatccgtt acggagtgct cgacaatgga ctaacatatt acattcgtca
     3841 taaccaagag ccaaaagaaa gggctagttt ctacattatt caaaacgtgg gcgctttgct
     3901 cgaagaggat aatcagaacg ggcttgcaca ttttcttgaa catatggcat tcaatggtac
     3961 caagcatttc gaagggaaag ggatcttgaa tactcttgaa aaacacgggg ttgcttttgg
     4021 gcggaacatt aacgcttata cctctttctc cgaaacagtt tacaacctta gcgaagtgcc
     4081 taccacccat gaaggcctgg ttgatacatg tttgcttgtt cttagcgatt ggtcacatta
     4141 tctgttactt acagataagg aaattgacaa tgaacgtggt gtccttacag aagaatggcg
     4201 tactcggcga aatgcaagtt tcagaatgag gagtcagtgg ttcccggtgc tttttaaagg
     4261 ctctcaa
//
LOCUS       DAOPUW010000077         4266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_20862_length_4266_cov_4.624792, whole genome shotgun sequence.
ACCESSION   DAOPUW010000077 DAOPUW010000000
VERSION     DAOPUW010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20862_length_4266_cov_4.624792"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PKJ43_02130"
     CDS             complement(<1..591)
                     /locus_tag="PKJ43_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNZ71387.1"
                     /translation="MKKVAIGVDIGGTNTAIGVVDKDGNVMVKTSISTPSHGDIDKYI
                     NELAEAIKEQINSVRLLNEEIEILGIGIGAPNGNYYKGTIEHAPNLSFKGIVPFVQLL
                     KEKFQSLENIALTNDANAAAIGEMIYGGAKGMKNFVMYTLGTGVGSGLVVDGNLVYGA
                     DGFAGECGHTMLVPDGRLCGCGAKGHLEAYYSAPGMK"
     gene            complement(875..2260)
                     /locus_tag="PKJ43_02135"
     CDS             complement(875..2260)
                     /locus_tag="PKJ43_02135"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ71388.1"
                     /translation="MSQQSNSNLKIGMTFMASIFFIFGFITNFNIAMKDQVQLAFTLS
                     NFGAQLVNFAFFIAYAVFSFLCGFIIKRIGYKNGVIAGLVLVAAGSYTFFPAVAALSY
                     PLFLLAVFIMASGVVFLQTAANPYVAALGPQETAPARLNLTQALNGVATTIAPFLAGV
                     LVLAPAVLAINSGSTPADAAKFVQGPFLVIGTLVLLIAIGISFIKLPAIIGDTTISSK
                     SVFKKPQVWLGAIGIFCYVGAEVGTASQIAPYLSDSGYATDVAVRLSAIYWGGAMIGR
                     FFGSILLGNMENRKKYQLSALIIVFAFFVGWFITSANFVDGKFIFESKIVNGFIFLGI
                     AVLNFLLMLLGKGKANLALGVFGTVNMLLIVAALLLPANIGMWGLLSIGFFNSIMFPN
                     IFALGVNDLDPSEMPLASGIINTLICGGAVIPLLMGFVTDSVSAKAALIISILCYAYI
                     TFFALKGSKIR"
     gene            2596..3483
                     /locus_tag="PKJ43_02140"
     CDS             2596..3483
                     /locus_tag="PKJ43_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71389.1"
                     /translation="MKLKDLFVPAWKHSNPNVRLKAIEKNNYDENILSCIIKNTDEEI
                     DVAFAAIDKLQNPELCEKLFQTVYGKNADEKRKKILQKITDEKRIHNIYWKIRNSGGK
                     SFLEIDLEERLFEIWKSEVEQTHDTDTLIAIAKKSLNHIQGSYKSCCSYGITHDAIKK
                     IPDLKKLSAWVLSQFALNQELHYSILNGLSERLKQKDSGKFFSTIDNTELLIFLYKNG
                     GTALVSGVEKRLSQMGKKIRVDEYDKEGKCSSCEGVGGGDARIPYTDNDWKWERCEYC
                     NGTGREIFHIKEYFVESLE"
     gene            complement(3512..>4266)
                     /locus_tag="PKJ43_02145"
     CDS             complement(3512..>4266)
                     /locus_tag="PKJ43_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HNZ71390.1"
                     /translation="EALEAQEAKGLRVTESLGLKSIEEMRSLPAESLLSINERFGLTT
                     DNVVIPSIYETFEKSEHNDTPLLTGWNADDGVSFGPGPSAEKFTEMARNNYGEKAERF
                     LQLFPAATDKQAEASQKLLSQLYFGWQNYTWARMQTRKGKNPAYVYFFTHVPPGEQPY
                     YGAFHSAEFAYALHTLKFWNRPFSEPDFQLEEIMSSYWANFAKTGNPNGEGLPEWPVF
                     NPDKPEVMDLGDNVKAISLPHQEQLSFLDEAQ"
BASE COUNT         1385 a          901 c          820 g         1160 t
ORIGIN      
        1 cttcatgcca ggagccgagt aataagcttc gaggtggcct ttagcaccac atccgcaaag
       61 cctgccatcg ggaacgagca tagtatggcc acattctccg gcaaaaccat cggcaccgta
      121 taccaagttt ccgtcgacaa ccaatccgct gccaacaccg gtccccaagg tgtacatcac
      181 aaagtttttc atgccttttg cgcctccata aatcatttca ccaatagctg ccgcattagc
      241 atcattggtg agggcaatgt tttccagcga ttgaaatttt tcttttagca gttgtacaaa
      301 aggaacaatg cctttgaacg aaagatttgg agcatgctca atagtccctt tgtagtagtt
      361 tccgttgggt gcaccaattc ctatcccaag tatttcaatc tcttcgttta gcaaacgaac
      421 cgagtttatt tgttctttaa tggcttctgc cagttcattg atatacttat cgatatctcc
      481 atgagaagga gtcgatatcg atgtttttac cattacattg ccatctttgt caacaacgcc
      541 aattgcggta tttgttcctc ctatatctac tcctattgct acttttttca tcttttttat
      601 cttattaagt tactgattag ttattgtctt acctgctaaa tttcaaacga tacattttat
      661 ataatgacca aatgaatttt taaaaataat aattattgtt tgtaattcta tgctgttcag
      721 ggttcatttc tgttttcaaa tttaattttt tgagttgaat gcacaacatt ccttccgctc
      781 aaaaggataa aaatagcctg aactaaaaac atccctgaaa acttttctgg aggaaagctc
      841 tcaaggatgt ttatctatat aataaatgtt tgatctatcg tattttactt cccttcaatg
      901 caaagaaagt aatataagcg tagcaaagaa ttgaaataat aagcgcggct ttagcactta
      961 ccgagtcggt tacaaaaccc atgagtaaag gaataactgc tcccccgcaa attagggtat
     1021 taattatacc cgaagccagc ggcatttctg aagggtcgag gtcgtttacg cccaacgcaa
     1081 aaatgttcgg gaacataatc gagttgaaga aaccaattga taaaagaccc cacataccaa
     1141 tattagccgg caacagaagg gcagccacaa taagcagcat attcactgtt ccaaaaactc
     1201 caagggcaag gtttgctttg ccttttccga gcaacataag caaaaagtta agtacagcta
     1261 ttcccaaaaa gatgaaccca ttaacgatct tcgattcgaa aataaatttg ccgtcaacaa
     1321 aatttgctga agttataaac catcctacga agaaagcaaa aactatgatc aaggcagaaa
     1381 gctggtattt ttttctgttt tccatgttgc caagaagaat tgaaccaaag aaacggccaa
     1441 tcatggcacc accccaataa atagctgata gcctaacagc aacgtcggtt gcataaccac
     1501 tatcgctaag gtaaggcgca atttgagaag cagtacctac ttcagcacct acatagcaga
     1561 aaataccgat agcaccaagc catacttgtg gctttttgaa aactgatttc gaagaaatgg
     1621 tagtatcgcc aataatagca ggaagtttaa tgaaactaat ccctatggca attagtaaaa
     1681 caagggtacc tataacaagg aatgggcctt gaacaaattt tgcagcatcg gcaggggttg
     1741 accctgagtt aattgccaac acagcaggag ccaaaaccag cactccggca aggaaagggg
     1801 caatagttgt tgccaccccg tttaacgctt gggtaagatt aagacgggca ggagctgttt
     1861 cttgtggccc taatgctgca acatacggat tggcagcggt ctgcaaaaac accaccccag
     1921 aagccataat gaaaacagcc aaaaggaaca atggatacga caacgcagct acagccggga
     1981 aaaacgtgta acttccggca gcaacaagca caaggccggc aattactcca tttttatacc
     2041 caatacgttt gattatgaag ccgcacaaaa agctgaaaac ggcatatgca ataaaaaaag
     2101 caaagttaac cagctgagcc ccaaaattgg aaagggtaaa cgccagctga acctggtctt
     2161 tcatcgcaat gttgaaattg gtgataaaac caaaaatgaa aaagatggac gccataaagg
     2221 tcattccaat ctttaggtta ctgttggatt gctgtgacat ttgagtactt ttaattaatt
     2281 tgttttatgg tttgtgtgaa tttacgtcga aacgaacaaa tttaaaaatt aaatcgaaat
     2341 tgatctatgc tctccgtcgt aaaactgcgt taacaattcc tatttcccca atatattttt
     2401 gacactcaat cgggatcgtt ttccattaca gaaatctttt tcatatacgc tacacccgaa
     2461 caatggaaca acaaaaatta aaccctttaa agagaataat tctcaacatg atcccaaaat
     2521 aattcatact tttgaataaa gagagacatt aaacctccag aaaatctttt gggtttttca
     2581 gcaaatacac cgccaatgaa acttaaagac ctttttgttc ccgcatggaa gcacagtaac
     2641 cctaacgtac ggttaaaagc catcgagaaa aacaactacg atgaaaatat tctttcctgc
     2701 attataaaaa acactgatga agagattgat gtcgcttttg cggctatcga caagctacaa
     2761 aacccggagc tatgcgaaaa acttttccag actgtttatg gaaaaaatgc agatgaaaaa
     2821 cgaaagaaga ttcttcaaaa gattactgac gaaaaacgca tccacaacat ttactggaaa
     2881 atacgtaaca gtggtggaaa atcttttctg gagatcgatc tcgaggaaag gttatttgaa
     2941 atatggaaat cggaggttga acaaacccac gatacagata ctctcattgc cattgccaaa
     3001 aaatcgctaa accacattca aggttcatat aaaagttgct gttcatacgg tatcacccat
     3061 gatgccatta aaaaaatacc tgatttaaaa aaactgtcgg catgggtatt gtctcaattt
     3121 gctttgaacc aagagctaca ttattccatt ctgaatggtt tgtccgaaag gctgaaacaa
     3181 aaagattctg gaaaattctt ttcaacgatc gacaataccg aattactaat ctttctatac
     3241 aaaaacggag ggactgctct tgtttcaggt gttgagaaaa ggctatctca aatgggaaaa
     3301 aagatcaggg ttgatgaata cgacaaagag ggaaaatgct cttcgtgcga aggagtaggc
     3361 ggaggagacg ccaggattcc ctacaccgat aatgactgga aatgggaaag atgtgaatac
     3421 tgcaacggaa caggccgcga aatatttcac attaaagagt acttcgtcga aagcctcgaa
     3481 tagaattcat tactacaatc atatcaagtt attactgagc ctcgtctaaa aatgatagtt
     3541 gctcctgatg aggtaaagaa atggctttta cattatcgcc taaatccata acctcgggtt
     3601 tatcgggatt gaagacgggc cactcgggca acccttctcc attgggattg cctgttttgg
     3661 caaagttggc ccaataactg ctcattattt cttcaagttg aaaatcgggt tcagaaaacg
     3721 gacggttcca gaacttaaga gtatgcaaag cataagcgaa ttcagccgag tgaaaagccc
     3781 catagtaggg ttgttctccc ggcggaacat gggtgaaaaa atatacgtac gccgggttct
     3841 ttccttttct tgtctgcatg cgggcccatg tataattttg ccatccaaaa tatagctggg
     3901 atagcagttt ttgcgaagcc tctgcctgct tgtcagttgc ggcaggaaaa agctgaagaa
     3961 aacgttcggc cttttcacca taattgttcc tcgccatttc tgtaaacttt tcggctgaag
     4021 gacctggtcc gaatgaaacc ccatcgtcgg cattccagcc cgtcagcaag ggcgtatcgt
     4081 tatgctcact tttttcaaaa gtctcataaa tcgatggaat gactacgtta tcggttgtga
     4141 ggccaaatcg ctcatttatt gaaagtaacg actcagcagg caacgaacgc atttcttcaa
     4201 ttgacttcaa tcctaatgat tctgttacgc gcaatccttt tgcttcttgt gcttcaagag
     4261 cttcgc
//
LOCUS       DAOPUW010000078         4261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_20892_length_4261_cov_3.303852, whole genome shotgun sequence.
ACCESSION   DAOPUW010000078 DAOPUW010000000
VERSION     DAOPUW010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20892_length_4261_cov_3.303852"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PKJ43_02150"
     CDS             <1..716
                     /locus_tag="PKJ43_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71391.1"
                     /translation="AFVSQSGIDAAIQNPAGVASLPAVAFAAFYEKRFTISELSSQGI
                     IGALPVGKGVFSACFHTFGPLSWRETEASLGYSLQLSPKLSGGVKINYLSMKLPEENA
                     QLGTASATVGLIYKLLPSLFIGLNIDNPWSVALQTSTFENNIPYKIQLGGHSKISENF
                     TVSAEVEKLDKEPVVLKMGAEWEAADHFFLRGGYSSSTTKLYGGLGFSYRYFTADLAF
                     GLHQTLGVTPFVSIKFNLN"
     gene            713..2758
                     /locus_tag="PKJ43_02155"
     CDS             713..2758
                     /locus_tag="PKJ43_02155"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNZ71392.1"
                     /translation="MKVRFIHIILIMLLVPATLFAQERSAFERSALLEDIAETAAENG
                     DENTDLSAVLEELEDLMNHPLSINNVSRTELEKLRFLSPAQIDNLLNYRNKVGQLFST
                     TELSLIDGFNDDLANKLTLFVQIEAPEEKDFNFSKNEVNAYTHYIIEKAKGFIPDENG
                     DKRFAGSMPKMLLKYKGEKSKHWNWGLTAENDMGEAFFSGNNKAGFDFYSGFVSWQGN
                     KLLRRIIVGDYQVKSGQGLVIWSGYGGRKTIDGMSMRYTGQGLRPYSSAAEYGFLRGV
                     AASFESGPLSFTTFYSNRNNDANVTIVDANDDPAAVSTITESGYHRTGSEIVDKGALN
                     IQTAGLNARYTKGTFLAGLNSGYQRYGTSVQPDPKLYNQYYFRGNENFCISADAQKAL
                     KNASLYGEFAVSKSGGTALVAGFDASPASEVALSVVFRDYKKDYHTISGNSFSEFGNV
                     SNERGLYSSITLYSIPRVTLNAYLDTYKSYWVKFSSLRPVSGYDFAVQGLWAISRKLN
                     LQLRYRTETRNENASIESTVKTDTYKTTNKIRLNTEWKATNTLTFRLRSEWNSVEKDD
                     SLLQGFMVLADANAKLFNEKLSATARVAWFDTDHYNSGIRTYENDLPLNFSVPVYYLK
                     GMRYYINLNYKASRALTIYLKLSQTLLNNEYTSIGSGDLAIEGKQKTEIKFQVRYKF"
     gene            2819..3958
                     /locus_tag="PKJ43_02160"
     CDS             2819..3958
                     /locus_tag="PKJ43_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNZ71393.1"
                     /translation="MMNFDEIRPFNNDELQSAIASLLADERFMRLVNYLFPEKQIKEQ
                     MLYVFSTIDNIDDFQSKIILPLLDNIKRTTTNGVTISTMDQFKRADNYLFISNHRDII
                     LDPAFLNMLMAQNGFRTTEIAIGDNLLIYDWITTLVRINRSFVVKRNLGLREQLTASK
                     ELSAYIRHTLTRKGESVWIAQREGRSKDGNDLTQPAILKMLNMSNDGSLLEGFYELNI
                     VPMAISYEIEPCGILKVEETLKRKFNPSFSKTPEDDIKSMSDGVTKPKGRVHFSFGNP
                     VNYKLIELLKDKNTGETIQTISEYIDKRIYFNYKLWPNNYIAHDLLFNSNSHAARYTK
                     EEKEQFVTMMNNEVSTIAFDKAEACSQYLQLYANPVINYEKHFGE"
     gene            3955..>4261
                     /locus_tag="PKJ43_02165"
     CDS             3955..>4261
                     /locus_tag="PKJ43_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNZ71394.1"
                     /translation="MIKTDTIIWDWNGTLLNDTEICIETINELLSKRGLPLIDKAKYH
                     ETFGFPVIDYYRRIGFDFSKEPFEIPALEYIALYETKVRNCSLHSEVPEILSYFESKD
                     "
BASE COUNT         1347 a          915 c          877 g         1122 t
ORIGIN      
        1 atgcctttgt cagtcagtcg ggtatcgatg cagccataca aaatcctgca ggagtcgctt
       61 ccctccccgc tgttgctttt gcagctttct atgaaaaacg atttacaatc agcgaattat
      121 cgtctcaggg cataattggc gccttacctg tcgggaaagg ggttttttca gcatgtttcc
      181 acacttttgg ccctttaagt tggcgtgaaa ctgaagcttc gctgggatac tcgcttcaac
      241 tctcccccaa actatcaggc ggtgttaaaa ttaattacct gtcgatgaag ttacccgaag
      301 aaaatgcaca gcttggtact gccagtgcta cagtagggct aatttacaag ctcctgccct
      361 ctctttttat aggactcaac atcgataatc catggtcggt tgcattacaa acctcgacct
      421 tcgagaataa tataccatac aagattcagc tcgggggaca ttcaaaaata agcgagaatt
      481 ttacggtttc tgccgaagtg gaaaaactag ataaagagcc tgttgtactg aaaatgggtg
      541 ctgaatggga agccgctgac cattttttct tgcggggcgg atacagctca tcaacaacaa
      601 aactctatgg aggcttgggg tttagctatc ggtacttcac cgctgatctt gccttcggac
      661 tacaccaaac gctaggggta acaccttttg tttcaataaa attcaacctg aattgaaagt
      721 acgtttcatc catatcatct tgattatgtt gctcgttccg gcaactcttt tcgctcagga
      781 aagatctgcg ttcgaacgat cggctctgct cgaggatata gcagaaaccg ctgctgaaaa
      841 tggagatgag aataccgatt tatctgccgt tcttgaagaa ttggaagacc tcatgaacca
      901 cccgctgagc atcaacaacg taagccgcac cgaattggaa aaactgaggt tcttaagtcc
      961 tgcacagatt gataatctat tgaattacag gaataaagtt gggcagttat ttagcacaac
     1021 agaacttagt ttaatcgacg gtttcaacga tgatttagcc aacaaactga ccctgtttgt
     1081 tcaaattgaa gcccctgaag aaaaagattt caacttcagc aaaaacgaag tgaatgctta
     1141 cactcattat attattgaaa aagccaaagg atttataccc gatgagaatg gcgacaaacg
     1201 ctttgccggc tcaatgccca aaatgctcct gaaatataaa ggagaaaaaa gcaagcattg
     1261 gaactggggc ttaactgctg aaaacgatat gggtgaagct tttttcagcg ggaataataa
     1321 agccgggttc gacttttatt cgggctttgt gtcgtggcag ggcaacaagc tcttacgacg
     1381 catcattgtt ggcgattatc aggtgaagag cggacaaggc ttggtgatat ggtcgggata
     1441 cggtggtaga aaaacaatcg acggcatgtc aatgcgatat acaggtcagg gattgcgccc
     1501 ctatagttcg gctgccgagt atggtttcct gcgtggtgtt gctgccagtt tcgaaagtgg
     1561 gccgctcagt tttaccacgt tctattcaaa caggaacaac gatgccaatg ttacgattgt
     1621 cgacgcaaat gatgacccgg cggctgtttc aaccataaca gaatcgggtt accaccgcac
     1681 aggcagcgaa attgtcgata aaggagcgtt gaacatacaa actgccggat taaatgcaag
     1741 gtacacaaaa ggaacatttt tggcaggatt aaactctggt tatcaacgct atggaacatc
     1801 agttcagcca gatccaaagt tatataatca gtattatttt cgcggtaacg agaatttttg
     1861 catcagtgcc gatgcccaaa aagccctgaa aaatgcttct ctttatggag aatttgcagt
     1921 cagtaaatcg gggggaacgg cgcttgtagc aggttttgac gcatcgccgg caagcgaagt
     1981 ggcactaagt gttgtgtttc gcgattataa gaaagattac cacacaatta gcgggaactc
     2041 gttctctgaa ttcgggaacg tatccaatga aagaggcctc tattcgagca taacattata
     2101 ttctatacct agggttacac tcaatgccta cctcgataca tataaatcgt attgggtgaa
     2161 gttttcctcg ctacgacctg tctccggtta cgattttgcg gtacaggggc tttgggcaat
     2221 ttcgagaaaa cttaatcttc aacttcgcta tcgcaccgaa acccggaatg aaaatgcttc
     2281 gattgaaagt actgttaaaa cagacactta caaaaccaca aacaaaatca ggcttaatac
     2341 cgaatggaaa gccaccaata cgttgacttt caggttacgt tcggaatgga acagtgttga
     2401 aaaagatgac tcactactac aaggttttat ggtattggcc gatgcaaatg ccaaattatt
     2461 caacgaaaaa ctaagcgcca cagcccgggt ggcatggttt gataccgatc attataactc
     2521 aggaataagg acatacgaaa acgatttacc gttgaatttt tcagtcccgg tatactacct
     2581 taaaggtatg cgctactata taaacctgaa ctacaaagcc tcacgcgcgt tgacaatcta
     2641 tttaaaacta tctcaaaccc tattgaacaa cgagtatact tctataggct cgggcgactt
     2701 ggccattgag ggaaaacaaa aaacagagat caaatttcag gttcgttata agttctgaca
     2761 cgaataattt tcacccttca tagttttcta taaatttgcg caacaaaaaa ataaggtaat
     2821 gatgaatttt gacgagatac ggcctttcaa taacgacgaa cttcaaagtg caatcgcttc
     2881 actgttggcc gatgaacggt ttatgagact tgtaaattac ttgttccccg aaaaacaaat
     2941 taaggaacaa atgctttatg tgttttcaac aatcgacaac atcgacgatt ttcaaagtaa
     3001 aattattttg ccattactcg acaacataaa aagaacaaca acaaatgggg tcactatcag
     3061 cacgatggat caattcaaac gtgccgacaa ttaccttttc atatccaacc atcgcgacat
     3121 catcctcgat ccagcatttc tcaacatgct catggcccag aatggatttc gtactactga
     3181 aattgctatt ggtgacaacc tgttgattta cgattggatt acaacactgg ttcgcatcaa
     3241 ccgttctttc gtggtaaaaa gaaaccttgg tttacgcgaa caactcacag cctcgaaaga
     3301 actttctgct tacatacgcc ataccctcac tcgaaaaggc gaatcggtat ggattgctca
     3361 acgcgaaggc cgctcgaaag acgggaatga cctaacccaa cccgccatcc tcaagatgtt
     3421 gaatatgagc aacgatggct ctcttctcga aggattctac gaattaaata tcgtacccat
     3481 ggctatttcg tatgagatag aaccttgcgg aatactcaaa gttgaagaaa cgctgaaaag
     3541 aaaattcaat ccttcgtttt caaaaacacc tgaagacgac ataaaaagca tgagcgatgg
     3601 tgtaacgaag ccaaaaggac gggttcattt ctcatttggc aacccggtaa attacaaact
     3661 gatagaatta ttaaaagata aaaacaccgg agaaactatt cagactattt cagaatatat
     3721 cgacaagaga atttacttca actacaaact ctggccgaac aattatatcg ctcacgacct
     3781 gcttttcaat agtaattcac atgctgcaag gtataccaaa gaagaaaaag agcagtttgt
     3841 gaccatgatg aacaacgagg tatcgaccat cgctttcgac aaagcagaag catgcagcca
     3901 gtatttacaa ctttatgcca accctgtaat taattacgaa aagcatttcg gagaatgatt
     3961 aaaaccgata ctattatttg ggattggaac ggcaccttgc tcaacgatac cgaaatttgt
     4021 atcgaaacca taaacgaact gctcagtaaa cgtggcctgc cacttatcga caaagcaaaa
     4081 tatcacgaga catttgggtt tcctgtaatt gattattatc gcagaattgg tttcgacttc
     4141 agcaaagaac cttttgaaat acctgcattg gaatacattg ccttgtatga aacaaaagta
     4201 cgtaattgca gccttcattc agaagtacca gaaatcctaa gctactttga atcaaaagac
     4261 t
//
LOCUS       DAOPUW010000079         4226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_21132_length_4226_cov_3.592184, whole genome shotgun sequence.
ACCESSION   DAOPUW010000079 DAOPUW010000000
VERSION     DAOPUW010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21132_length_4226_cov_3.592184"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PKJ43_02170"
     CDS             complement(<1..201)
                     /locus_tag="PKJ43_02170"
                     /inference="COORDINATES: protein motif:HMM:NF036998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71395.1"
                     /translation="MIIRDFTDNDLYKFTTMNAIQKKFPKAEVKYQFINRGKTQFPDG
                     FAKALRQEVDAMASLTLSDEAEA"
     gene            complement(232..930)
                     /locus_tag="PKJ43_02175"
     CDS             complement(232..930)
                     /locus_tag="PKJ43_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNZ71396.1"
                     /translation="MRQSSYCYEYPRPAVTADCIIFGFDAGTLKVLLIERGIEPFKGH
                     WAIPGGFLRENESADDCAKRELFEETGLENVFMEQLFSFTEPGRDPRGHVITVAYYAL
                     VSLMEYELKAGDDARNAAWFPIDQLPALAFDHDRILSFALNRLRGKIRYQPIGFELLP
                     ERFTLPDLQALYEAILQVKIDRRNFRRKINQTGLLIDHNESVTGVPHRGARYYSFDKA
                     KYEQLAQRGFNFEI"
     gene            1119..1811
                     /locus_tag="PKJ43_02180"
     CDS             1119..1811
                     /locus_tag="PKJ43_02180"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HNZ71397.1"
                     /translation="MNTRITNRLLVALPLIFFSLDVFARAGGGGGSGSSGGGKGNLIA
                     LILVGIYSFIVTVALYIKVDKSRTVIENGEINGSFWNFEQMRDHSKRVFLKMQHAWMN
                     RDINRVKDLISPRLYEDLKAHLEFMTENGEKNILSGIDIKSIKIIGCEDYLDNNQDSF
                     IAHIKGSMLDYTINEKTSQVIKNADKSVETFTDTYHFIRTDNQWLLDYIDNHVSIWDI
                     IKTRNYYETSRN"
     gene            2114..2554
                     /locus_tag="PKJ43_02185"
     CDS             2114..2554
                     /locus_tag="PKJ43_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNZ71398.1"
                     /translation="MSRVSTYLNFSRNTEEAFQFYKSVFGGEFSGGGIMRMGDVPPQD
                     GVPPLAEEDKNLIMHIELPILDGHKIMGTDAPESMGFSVKFGNNQYITLDPDTRAEAK
                     RIFAALSEGGKIEAELAEMFWGDYYGSFTDKFGVQWMINCTARE"
     gene            2559..2981
                     /locus_tag="PKJ43_02190"
     CDS             2559..2981
                     /locus_tag="PKJ43_02190"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNZ71399.1"
                     /translation="MDKAKIAITVQAAIAAPVDKVWNCWTDPSHIVKWNSASDDWHTP
                     NATNDLRPGGKFTSRMEAKDGSFGFDFEGVYDEVILNEYIEYTIADGRKVKIVFNRNG
                     DHCEVVETFEAETQNSVELQREGWQAIMNNFKKYVENS"
     gene            2983..3465
                     /locus_tag="PKJ43_02195"
     CDS             2983..3465
                     /locus_tag="PKJ43_02195"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNZ71400.1"
                     /translation="MELAKTPIIPCLWFDKQALEAATFYTSIFKNSSIGKISRYTGEG
                     KEIHRQEEGTVLTVEFTLNGQPFVALNGGPVYSFSEAVSFQIFCSSQEEIDYYWEHLT
                     ADGGEESYCGWLKDKFGLSWQVVPAILTELLSDPERASRVTRAFMQMRKFDIEILTKA
                     "
     gene            3736..>4226
                     /locus_tag="PKJ43_02200"
     CDS             3736..>4226
                     /locus_tag="PKJ43_02200"
                     /inference="COORDINATES: protein motif:HMM:NF021540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HNZ71401.1"
                     /translation="MKRKVLFIFVIVLLSINGMAQTAGELKVDLMTSGTGGRYTPKNS
                     MAIWVEDESGKFVKTLVAHAEKRKIFLTNWKSSTSKAGAEFNTVDAISSATRNSHDKV
                     SCTWNGKDFNQKLVADGKYKICIELTEINGTGVYSSFIINKGRSAKTVKPANVPSFSD
                     VSLV"
BASE COUNT         1339 a          840 c          890 g         1157 t
ORIGIN      
        1 ggcttcggcc tcatcgctta aagtcagcga tgccatagca tccacttcct ggcgcaaggc
       61 tttggcaaat ccgtcgggga actgggtttt gccgcggttt ataaactgat atttcacttc
      121 cgctttcgga aatttctttt gtatcgcgtt cattgttgtg aacttataca aatcgttgtc
      181 ggtaaaatcg cgaatgatca taggtttgtc tttttatgtt attgtttgat tttatatttc
      241 aaaattgaat ccccgctggg caagctgttc atattttgcc ttgtcgaaac tataataccg
      301 ggcccctcgg tgcggaaccc ctgtcaccga ttcgttgtga tcgatgagta aacccgtttg
      361 gtttattttc ctgcggaaat tgcgccgatc gatcttcacc tgcaaaatag cttcgtacaa
      421 agcctgcaaa tcgggcaagg taaaacgctc gggcaacaac tcgaacccaa tgggctgata
      481 gcgaatcttc ccacgtaaac ggttcagggc gaaactcagg atgcggtcgt ggtcaaaagc
      541 caaggccggc agctgatcga tggggaacca agccgcatta cgtgcatcgt cgcctgcttt
      601 tagctcgtac tccatcaaac tcaccagggc gtaataggcc actgtaatta catgccccct
      661 aggatcgcgc ccgggttcgg taaacgagaa aagctgctcc atgaaaacat tttcgagacc
      721 agtttcttca aacaactcac gtttggcgca gtcgtcggca ctttcgttct cacgcaaaaa
      781 accgcctgga attgcccaat gccctttaaa cggttcaatt ccgcgttcaa tcagcaacac
      841 cttcaaggtg ccagcatcga acccaaaaat aatacagtcg gcagttacag ccggtctcgg
      901 atattcataa cagtaggatg attgtctcat agcatcaaaa tgttattttg acacaaaggt
      961 atacttctaa aataaacaac caaattttct tggtgttatt ttgacacaaa gacttattat
     1021 tttacaaata cttcaatatt tggttcatta tgaatgaata acaaaagaag aaaaaatgta
     1081 agtttggaac agaaaccaaa aattattctt accattttat gaatactcga atcactaacc
     1141 gacttttagt ggcattgcca ttgatattct tctcattaga tgtttttgcc cgggcaggtg
     1201 gcggaggcgg aagcggaagt agtggcggag gaaaaggaaa cctgatagcg ttgatacttg
     1261 ttggcatata ttcattcata gttaccgtgg cattatatat aaaggttgac aaaagccgaa
     1321 cagttattga aaacggggaa ataaacggtt cgttttggaa cttcgaacaa atgcgcgacc
     1381 attctaaacg ggtcttttta aaaatgcagc atgcctggat gaaccgcgat ataaaccggg
     1441 taaaagatct gattagtcca cgtctgtacg aagaccttaa agcccacctt gaatttatga
     1501 cggagaatgg agaaaagaat atcttatcgg gcatcgatat aaaaagtata aaaattattg
     1561 gatgtgaaga ttaccttgac aataatcagg acagctttat tgcacatatt aagggatcta
     1621 tgctcgatta taccataaac gaaaaaacca gccaagtaat aaagaatgcc gataagagtg
     1681 ttgaaacatt taccgacacc taccatttta tccgtactga caaccaatgg cttctcgatt
     1741 atattgacaa ccatgtaagt atatgggata tcataaagac aagaaactat tacgaaactt
     1801 cgagaaactg aaagacagac tattaggact tcttcaattc ataaaattaa acccttccct
     1861 actttagaat caattaacaa ttggttcgta ttgtgactca gattttggat gtgcttaaaa
     1921 catctattct attaaatatc aaaacattat cttttgataa aattcgacaa gaatcatttt
     1981 cgacatttca agactggaag gcatcatact aacaattctc tttaccgata actccatata
     2041 aaaaggaggt agacttagtg aacaatggtt cagcaaactt gttactttaa actataattt
     2101 aaaatctaca attatgtcac gagtaagtac ttacctgaat ttttcaagaa atacagaaga
     2161 agcgttccaa ttttacaagt cggtttttgg cggagagttt agtggcggcg gtatcatgcg
     2221 catgggcgat gttcctccgc aggatggcgt acctccactg gcagaggagg ataaaaacct
     2281 tattatgcac attgaattac ccatcctcga cggccataaa attatgggaa ccgatgcccc
     2341 cgagtcgatg ggtttcagcg ttaaatttgg caacaaccag tacattaccc tcgatcctga
     2401 tacaagggct gaagccaaac ggatatttgc agccctttca gaaggcggaa aaattgaagc
     2461 cgaacttgcc gagatgttct ggggcgatta ctacggtagt tttaccgata agtttggcgt
     2521 tcagtggatg attaactgca ccgcgaggga atagtgctat ggataaagcg aaaatagcga
     2581 tcacggtgca ggcggccatt gctgcacctg tcgataaagt ttggaattgt tggaccgatc
     2641 catcacacat tgtgaaatgg aacagtgctt cggatgactg gcatacaccc aatgcgacga
     2701 atgatctccg gccaggaggg aaatttacaa gcaggatgga agctaaagat ggaagttttg
     2761 ggttcgactt cgaaggggtg tacgatgaag taattcttaa cgaatacatt gaatacacaa
     2821 ttgctgacgg tcgaaaagtg aaaattgttt tcaatcgaaa tggcgaccac tgtgaagtag
     2881 ttgaaacctt tgaagctgaa actcaaaatt cggtcgagct gcagcgggaa ggctggcaag
     2941 ccattatgaa caactttaaa aaatacgttg aaaatagtta gtatggaatt agcgaaaacc
     3001 ccaattattc cctgtctatg gttcgataaa caggcacttg aagcggcaac gttttacacg
     3061 tcaattttta agaattcatc aataggaaaa atcagccgct acaccggaga agggaaagaa
     3121 atacaccgac aggaagaagg cacggtactc accgttgaat ttacccttaa tgggcaacct
     3181 ttcgttgcat tgaacggtgg accggtttat tccttcagtg aagctgtatc gtttcaaatc
     3241 ttttgttcct cgcaggaaga gattgattac tattgggaac atctcactgc cgatggaggg
     3301 gaagagagct actgtggatg gctaaaagat aagttcgggt tatcgtggca ggtggttcct
     3361 gcaatactta ctgaattact cagtgatccc gaaagagcgt caagagtaac cagggctttt
     3421 atgcaaatgc ggaaatttga tattgagata ttgacaaaag cgtaaaatca tcaaaaagtt
     3481 atagccaata attctgcttt tgtaactact caatcggcaa tcaatacttc gattaggctg
     3541 acccataccc cattgtaaaa aatcctgtca gatttcatta tccacttcta ctaaaaaaat
     3601 aacatgtttt tattccaatg ccaatagaaa gatataaacc ttcatagggg aaaagagtca
     3661 aatcaacaat ttttgtgttc ttttgtggga acacgctgta ttattaagat tcactaattc
     3721 ttagaattta ataccatgaa aagaaaagtt ttattcatat tcgtaattgt cttattgtca
     3781 atcaatggca tggcacaaac agcaggggaa ttaaaagttg atttaatgac ttcgggcact
     3841 ggcggaaggt acaccccaaa gaattcaatg gctatttggg ttgaagatga gtctggtaaa
     3901 tttgtaaaaa cccttgtagc ccatgccgaa aagagaaaaa tctttcttac caattggaaa
     3961 agctcaacca gcaaagccgg tgcagagttc aacactgttg atgcaatctc ttcagcaacg
     4021 cgcaacagtc acgataaagt atcatgcaca tggaatggaa aagatttcaa ccaaaagctt
     4081 gtagccgacg ggaaatacaa aatatgcatc gaactcacag agatcaatgg tactggtgtg
     4141 tattcttctt ttataatcaa taaaggccgt tcggcaaaaa ctgtaaaacc ggccaatgtt
     4201 ccaagctttt cagatgtcag cttggt
//
LOCUS       DAOPUW010000080         4224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_21148_length_4224_cov_2.917966, whole genome shotgun sequence.
ACCESSION   DAOPUW010000080 DAOPUW010000000
VERSION     DAOPUW010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21148_length_4224_cov_2.917966"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PKJ43_02205"
     CDS             <1..724
                     /locus_tag="PKJ43_02205"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNZ71402.1"
                     /translation="INPKQIYNKVMAIAEKVTPHDSSNMRKMLTKEGIQWSLTHGETW
                     WQNAKCIAVDGIHNAIKFLERLENGNVPNVDFLEIRACDRSCAGGILLTQNRFLTVER
                     LKNRAKKYPSSEKMIQFENYNFDELERKMITDEILPRPMLRFGSDMAKAINRMERARS
                     IMCHLPGIDCAACGAPSCQALAEDMVNGQARMSDCIFIEQLWENDGKILPQKAFERME
                     KKWGVGRFDPDCSKKGAKNEGF"
     gene            711..1043
                     /locus_tag="PKJ43_02210"
     CDS             711..1043
                     /locus_tag="PKJ43_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HNZ71403.1"
                     /translation="MKVSELIEKLGLTVFTGAVGVEREIAGGYTSDLLSDVMGNAKEG
                     NVWITLQNHMNVIAIASLKELACIILVKGVTPAPEIIEKAIAEEIPVLGSNENTFNLN
                     GKIYALLN"
     gene            1045..1776
                     /locus_tag="PKJ43_02215"
     CDS             1045..1776
                     /locus_tag="PKJ43_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNZ71404.1"
                     /translation="MASFKADMHIHTVLSPCGSLEMSPLNIVQSALDAKLDIIAITDH
                     NSTRQVAIVKHVAQKKGLFAIGGAEVNSAEEVHCLALFETDEQLSIFQTFIDKHILKI
                     QNKPDFFGYQVVVDEDENITYEEPWLLINALDAGILEIEKLVHSLDGLFIPAHIDRQF
                     NGIISQLGFIPDELKCDALELSPNAKTEYWKDDKRKSNNNIFIRNSDAHTPRDIARQF
                     TILEMEDISFQSIRKALLSNSLHIH"
     gene            1807..2355
                     /locus_tag="PKJ43_02220"
     CDS             1807..2355
                     /locus_tag="PKJ43_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ71405.1"
                     /translation="MTELSLHILDIVQNSIRAEANTIEITINEQTSEDRYTITITDNG
                     SGMDEETLKNAIDPFFTTRTTRKVGMGLSLFKQNAELSGGTMTVESKLGQGTKVSALF
                     GFSHIDRLPLGDMAGTMTLLIGANPKIRFIYNHITQLSDFEFDTNEVLEELGKVPINH
                     PDILKALKEMIEENLDMIEAAK"
     gene            2401..2796
                     /locus_tag="PKJ43_02225"
     CDS             2401..2796
                     /locus_tag="PKJ43_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNZ71406.1"
                     /translation="MKITSLADLRKIKDEVQEKIKLRELGENAEIHVQIKVAMATCGI
                     AAGAKDTMNYLIAELDSRGIDAIVTQTGCMGFCYAEPTIEVIKPGKEPVIFGFVTREK
                     AKEIIDEYIIKGELIDGVIPVNYKTIEEQ"
     gene            2815..>4224
                     /locus_tag="PKJ43_02230"
     CDS             2815..>4224
                     /locus_tag="PKJ43_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNZ71407.1"
                     /translation="MANYKMHILVCGGTGCRASQAEQIVKNFKQELAQKSLDKEIKVV
                     TTGCFGFCEKGPVVKITPDNTFYTQVTPQDVTDIVEEHFIKGRPLKRLLYHNPKNEFS
                     VTSAKNIDFYKKQVRIALRNCGFINPENIEEYIARDGYAALGMALTEMTPEQAIQLVK
                     DSGLRGRGGGGFPTGLKWEITRKVENSQKYVVCNADEGDPGAFMDRSILEGDPHSIIE
                     AMAICGYCIGASNGLVYIRAEYPLAIHRLKIALNQAKEYGLLGKNIMGTGFNFDIEIR
                     YGAGAFVCGEETALIHSMEGSRGEPTFKPPFPSVSGYQGKPTNVNNVETFANIPVIFN
                     KGAEWFNKIGTEKSRGTKVFALAGKVNNVGLIEVPMGTTLREVIFDIGGGIRGGKRFK
                     AVQTGGPSGGCLTEKHLDTPIDYDNLISAGSMMGSGGMIVMDEDDCMVSIAKFYLEFT
                     LDESCGKCTPCRIGNKRLFE"
BASE COUNT         1423 a          885 c          916 g         1000 t
ORIGIN      
        1 cataaacccc aaacaaattt acaacaaagt gatggccatt gccgaaaagg taacgcctca
       61 cgacagttca aacatgcgta aaatgctcac caaagaagga attcaatgga gccttaccca
      121 tggcgaaacc tggtggcaaa atgcaaagtg cattgctgtt gacggcatac acaatgccat
      181 aaaatttctt gaaagattgg aaaacgggaa tgtacctaat gtcgactttt tagaaatcag
      241 agcctgcgac cgaagttgtg ccggaggcat tttactgaca caaaaccgtt ttcttactgt
      301 tgaacgatta aaaaacaggg caaagaaata cccatcatca gagaaaatga ttcaattcga
      361 gaattacaac ttcgatgaat tggagagaaa aatgataacc gacgaaattt taccacgccc
      421 aatgctgcgt tttggcagcg atatggccaa ggcaataaac cgtatggaaa gggcccgaag
      481 cattatgtgc catctcccgg gcatcgattg cgctgcttgt ggggcacctt cgtgccaagc
      541 cctggctgaa gacatggtca acggccaggc ccgaatgtcg gattgtattt ttatagaaca
      601 gctttgggag aatgacggaa agattcttcc ccaaaaagct tttgaacgaa tggaaaaaaa
      661 atggggcgtt ggacgattcg accccgattg tagtaaaaaa ggagcaaaaa atgaaggttt
      721 ctgaattaat tgaaaagtta ggattgacgg ttttcactgg agccgtaggc gttgaacgcg
      781 aaattgcagg cggatatact tccgacctgt tgagcgatgt tatgggaaac gccaaagaag
      841 gtaatgtgtg gatcacctta caaaaccaca tgaatgttat agccattgcc tcgttaaaag
      901 agttggcctg cattattctg gtaaaagggg ttacaccagc acccgaaatc attgaaaaag
      961 ccatcgccga agaaattccg gttcttggaa gtaatgaaaa tacgttcaac ctgaacggga
     1021 aaatttatgc attattgaat taaaatggca agcttcaagg ccgatatgca tatacataca
     1081 gtcttgtctc cttgcggaag tttggagatg agcccgttaa atatagtaca atcagccctt
     1141 gatgcaaaac tcgacatcat tgcaataacc gatcataact ccacccggca agttgcaatt
     1201 gttaagcacg ttgcacaaaa aaaaggcctt tttgccattg gtggtgccga ggttaacagc
     1261 gcagaagaag tgcattgcct agccctcttc gagacagacg aacaactcag catatttcaa
     1321 acgttcatcg acaaacacat cctgaagatt caaaataaac ctgatttttt cgggtatcaa
     1381 gttgttgtcg atgaagatga gaatataaca tacgaagagc catggcttct gataaacgcg
     1441 cttgacgcag gtattctcga aattgaaaag ttggtacatt cgctcgacgg gttatttatc
     1501 cccgcacata tcgaccgcca gttcaatgga attatcagtc aacttggctt tattcccgat
     1561 gaactgaaat gcgatgctct tgaattatcg cccaacgcaa aaacagagta ttggaaagat
     1621 gacaagagaa aaagcaacaa caatattttt atacggaact ctgatgcaca tacgccacgc
     1681 gatattgccc ggcaatttac gatattagag atggaagaca tctcttttca atcgatacga
     1741 aaagcactac tatcaaattc tttacacata cattaaccaa ttggtaactg gtaaaaccaa
     1801 tacgctatga ccgaattatc gttacatatt ctcgacattg tccaaaactc cataagagcc
     1861 gaagctaata caatagaaat cactataaac gaacaaactt ccgaggatag atatacaatt
     1921 accattacag ataacggtag cgggatggat gaagaaaccc tcaaaaacgc catcgacccg
     1981 ttctttacta cacgcactac ccgaaaagtg ggtatggggc tctctctttt taagcaaaat
     2041 gcagaacttt cgggcggaac tatgaccgtg gaatcaaaac ttggccaggg aacaaaagta
     2101 agtgcactat tcggtttttc ccacatcgac cgcttgccct taggcgacat ggctgggaca
     2161 atgactttac tcataggagc taaccctaaa attcgcttta tttacaacca tattacacaa
     2221 cttagcgatt tcgaattcga cacaaacgaa gtacttgaag aactcggtaa agttccaata
     2281 aaccacccag atatattaaa agcgctgaaa gaaatgattg aagaaaatct tgatatgatt
     2341 gaagcagcaa aataagatca aaacataaaa ttaaataccg atcaacgaat cgtaaatatc
     2401 atgaaaatta catcgttagc cgatttacga aaaatcaagg acgaagtgca ggaaaaaatt
     2461 aaacttcgcg aattaggtga aaatgctgaa atccatgttc agataaaagt agccatggca
     2521 acttgtggaa ttgcagccgg agcaaaagac actatgaact atcttattgc tgagctcgac
     2581 agtcgtggaa tcgatgccat tgtgacccaa accgggtgca tggggttttg ttatgctgaa
     2641 cctaccattg aagtaataaa accaggcaaa gaaccggtga tatttggctt cgtcactcgt
     2701 gaaaaagcaa aagaaataat tgacgaatac attatcaaag gagaacttat cgacggggta
     2761 attccggtta attacaaaac aatcgaagaa cagtaaccct caaatacaac tcaaatggct
     2821 aattataaaa tgcacatttt ggtatgcgga ggtaccggat gcagggcctc acaagctgag
     2881 caaattgtga agaacttcaa acaagaactc gcgcaaaagt cactcgacaa agaaataaaa
     2941 gtggttacta ccgggtgctt cggattttgc gaaaagggac ctgtagttaa aatcacaccc
     3001 gacaatacct tttacacaca ggttacacct caggatgtaa ccgatattgt agaagaacac
     3061 ttcatcaaag ggcgaccatt aaagagattg ctttaccaca acccgaaaaa cgaattcagt
     3121 gtaaccagtg caaaaaacat cgatttctac aaaaaacaag tacgaatagc cttacgtaat
     3181 tgtggtttta tcaaccccga gaacattgag gaatacattg cccgcgacgg atatgcagct
     3241 cttggaatgg ctctaactga aatgactcca gaacaagcca ttcaactggt taaagattcg
     3301 gggctcagag gaaggggcgg cggtggtttc cctaccggac taaaatggga aataacacga
     3361 aaagtagaaa attcacaaaa atacgttgtc tgcaatgccg atgaaggtga cccgggagca
     3421 tttatggacc ggtcaatcct cgaaggcgac ccccacagca taatcgaagc catggcaatt
     3481 tgcggctatt gcattggggc atcgaacggg cttgtatata tacgtgcaga atacccgctg
     3541 gctattcacc gcctgaaaat agcgcttaac caagccaaag aatatgggct cttgggcaaa
     3601 aacataatgg gtactggttt caatttcgac atagaaatac gatacggagc cggtgctttt
     3661 gtatgtggtg aagaaacagc cttgattcat tcaatggaag gatcgagggg cgaacccaca
     3721 ttcaaaccac cattcccctc ggtatcagga tatcagggca agccaaccaa cgtaaacaat
     3781 gtagaaacat ttgccaacat cccggttatt ttcaacaaag gagctgagtg gttcaacaaa
     3841 atcggcaccg aaaaatcgag aggaacaaaa gtttttgccc ttgccggaaa agttaacaac
     3901 gtgggactga ttgaagttcc tatgggcaca accctgcgcg aagttatatt cgacatcggc
     3961 ggaggtattc ggggtggaaa acgcttcaaa gcagttcaaa ctggcgggcc atcgggagga
     4021 tgccttactg agaaacacct tgatacccct atcgattacg acaacctaat ttcagccggt
     4081 tcgatgatgg gctcgggagg aatgatcgtt atggatgaag acgactgtat ggtttcgatt
     4141 gctaaatttt atcttgaatt tacgctcgac gaatcatgtg gaaaatgtac accctgccgc
     4201 atcggtaata agcgcttatt cgag
//
LOCUS       DAOPUW010000081         4217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_21196_length_4217_cov_3.450985, whole genome shotgun sequence.
ACCESSION   DAOPUW010000081 DAOPUW010000000
VERSION     DAOPUW010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21196_length_4217_cov_3.450985"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1484)
                     /locus_tag="PKJ43_02235"
     CDS             complement(24..1484)
                     /locus_tag="PKJ43_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HNZ71408.1"
                     /translation="MKAVLFALTTVALLSGCSGQKAESPPEKSFSPEGKSVAVYTTAQ
                     NSNYKLSLAGLTEFKNADQTIETETFVFVNPENTFQTFMGIGGAITDASAEVFAKLPE
                     SKQQEFLSAYYDLEKGIGYTLARTNINSCDFGSESYTYIEEGDKELKTFDIAPDKQFK
                     IPMIKKATEAAGGKLPLYASPWSPPSFMKTNNSMLKGGKLLPEYADSWAKYYTKFIKA
                     YEAEGLPIWGISVQNEPLAVQTWESCIYTAEEERDFLKNHLGPVMQKEGLADKKIIVW
                     DHNRDMINERANTILSDPEAAKYVWGVGFHWYETWAGGKPMFENVGNVHKSFPNVNLI
                     FTEGCAERFVTGDYNNWNNGERYGESMINDFNNGTVAWTDWNILLDQNGGPNHKGNFC
                     YAPIHADTQTGKIFLTPSYYYIGHFSKFIRPGAKRVSTSCSRSNLKSTSFINTDDKIA
                     TIVMNNSEVQVNYRLKIKLQEIKMSILPHAIQTLVY"
     gene            complement(1627..3237)
                     /locus_tag="PKJ43_02240"
     CDS             complement(1627..3237)
                     /locus_tag="PKJ43_02240"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNZ71409.1"
                     /translation="MKKLIIALSLIAIFQACTTDFIELTPKVSKLESNAYITENDAFL
                     AITAVYDALSVQNWSFVPIMSDIKSDDAYTGGDASGSDMEYWQEQERFNISLESDAPK
                     NLWNRCYSGIYRANLFIERAEGIQWKTNGLKDRMIGEAKFLRAYFYWDLVRHYGWAPI
                     INKNLESVDAYKNLTQSTPEEVYSQIADDLLEAEAKCPTSVSSDELGRATKYAAQSLM
                     ARIYLYYQGFAKPVFGITNEWKSTDGKTIDKAYVQNALKSFINSNAHDLMPTYAEVFD
                     WVNDHNTKESVFTWQYAEKSKVGDWGNIWNVNGNFSVIFYGPRSAYLASDGSVMSEGW
                     SFGIPTWSLYNEFEEGDVRRDVSLFDDDLLSKYTIGFQNTGYFNAKFMPQKIYESTGG
                     GSPQLNWLLNYPDIRYADVLLMAAELFLTDDAGFALQCYNRVRTRAMGELAAKGSITL
                     DDIFHERRVELSGEGHRYWDLLRRGLNVAEQKINGSFQNLPAGIPNEADFEPRNFDAQ
                     SYGMFPIPASEIRNMNANTLKQFIPAYQ"
     gene            complement(3260..>4217)
                     /locus_tag="PKJ43_02245"
     CDS             complement(3260..>4217)
                     /locus_tag="PKJ43_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNZ71410.1"
                     /translation="DWLITAPVPMLVGNSAPTINGGSVNNKGLELEMGYKARAGNVNI
                     DVKVTGAFNRNEVIDIPNTEKVIVGGSGGFGQGNIVRFEVGQPAAFFWGYKTDGLFQT
                     ADDVANHTTTDSLGRTFKLQPNAKPGDVRFVDTNKDGLLTDLDRVNLGNAMPEFTGGL
                     NLSLDWKGIDFNMFWYVALGQETWMVLRRYDQWTTNYTKEMYDGRWTGEGTSNKYPRL
                     AKNDLNFNGNWKTPSDLLVYDSSFGRLRSLTVGYTFPKSLTQKAKIENLRFYVMGENL
                     ITLTNYPGFDPEIGSNNTFGAGVDRGVYPQPKTITFGMNITF"
BASE COUNT         1075 a          895 c          897 g         1350 t
ORIGIN      
        1 ggttttctat agtatgatct ctcttaatat acaagtgttt gaatagcatg gggcagaatg
       61 ctcattttga tctcctgcaa tttgattttt aaacggtaat ttacttgcac ttcgctgttg
      121 ttcattacta tggttgcaat tttgtcgtcg gtatttataa acgaagttga tttaagatta
      181 ctacggctgc aacttgtgct aacccgtttt gcccctggcc tgatgaattt tgaaaaatgg
      241 ccaatgtaat aatacgatgg ggtgagaaag attttcccgg tctgtgtgtc ggcatgaatt
      301 ggtgcatagc agaaatttcc tttatggttt ggtcctccgt tttggtcgag aagtatgttc
      361 caatcggtcc aagccacagt tccgttgtta aaatcgttaa tcatactttc gccatatcgt
      421 tcgccgttgt tccagttatt ataatcgcct gttacaaaac gttcggcaca accttcagtg
      481 aaaataaggt taacattagg aaatgatttg tgaacgttgc caacattttc gaacattggc
      541 ttgcccccgg cccaagtctc gtaccagtga aagccaacgc cccaaacgta ttttgccgct
      601 tcggggtctg ataaaatggt gttggctcgc tcgttaatca tatcgcgatt gtggtcccaa
      661 acaattattt ttttgtcggc aagtccttct ttttgcatca caggccctag gtgatttttc
      721 agaaagtcac gttcttcttc ggctgtatat atgcatgatt cccatgtttg tacagccaga
      781 ggttcgtttt gtactgaaat accccagatg ggcaagcctt ctgcctcgta ggcttttatg
      841 aattttgtgt aatatttagc ccatgaatcc gcatactcgg gaagcagttt gccacctttc
      901 agcatcgaat tgtttgtttt catgaatgag ggcggactcc acgggctagc atacaggggt
      961 agttttccac cggctgcttc tgtggctttt ttgatcatcg ggattttaaa ttgcttatcg
     1021 ggcgcaatat cgaatgtttt caactctttg tcgccttctt caatatatgt atagctttca
     1081 cttccaaaat cgcagctgtt aatattggtc ctggccaagg tatagccgat tcctttttca
     1141 agatcataat aagcgctaag aaattcttgc tgttttgact ctggcaactt ggcaaaaact
     1201 tcggctgaag catcagttat tgcacctcct atgcccataa aggtttgaaa tgtattttca
     1261 gggtttacga atacaaaagt ttctgtttca atagtttgat ctgcattctt aaattcagta
     1321 aggccagcca gtgaaagctt gtaattgcta ttttgagcgg ttgtataaac tgctaccgat
     1381 tttccttcgg gagaaaatga tttttctggg ggagattcgg ctttttgtcc gctacagcct
     1441 gataatagag cgactgtggt taacgcgaat agtactgctt tcataagtat aaacggttgt
     1501 aatttactgt ataattgtgt tttcggcatc aggccgaagt ctattttcaa gcaaaaggaa
     1561 gttaagtggc cagtcatttt ttactggcca cgttacctat ttttaagact tcctcttctg
     1621 ttttatctat tgataagcag gaataaattg ctttagggtg tttgcattca tgtttctgat
     1681 ttcagaagca ggaattggga acattccata cgattgagca tcgaaattgc gtggttcaaa
     1741 gtcggcttcg ttaggaatac ctgcaggcag gttctgaaag ctgccgttga ttttttgttc
     1801 agcaacattt aacccgcggc gtagcaagtc ccagtaccgg tgtccttcgc ccgaaagttc
     1861 aactcgtctt tcatgaaaaa tgtcatcaag ggttatcgag cctttagcgg ccagctctcc
     1921 catggcacgg gtgcgaaccc tgttgtaaca ttgcagagca aaaccagcat cgtcggtaag
     1981 gaacaattca gcggccatca gcagaacatc agcataacga atatcggggt aattcaacaa
     2041 ccagtttaac tgaggtgatc cgccacctgt tgattcatag atcttttgag gcataaactt
     2101 ggcattgaaa tatccagtgt tctggaatcc gatagtatat ttgcttagca ggtcatcgtc
     2161 gaaaagcgaa acatcacggc gaacgtcgcc ttcttcaaac tcgttgtaca aactccaggt
     2221 aggaatacca aagctccatc cttcagacat tacagatcca tcagaagcga gatatgccga
     2281 gcgaggtcca tagaatataa cagagaagtt cccgttaaca ttccagatat tgccccagtc
     2341 gcctactttc gatttctcgg catattgcca tgtaaacacg ctttctttgg tgttatggtc
     2401 gttcacccaa tcgaacacct cggcataggt tggcataagg tcgtgtgcat tgctgttgat
     2461 gaacgatttc aatgcatttt gcacgtaagc tttgtcgatg gtttttccgt cggtcgattt
     2521 ccactcattg gtgatgccaa atactggttt ggcaaatcct tggtaataaa gatatatgcg
     2581 ggccattaag ctttgagctg catattttgt tgctctcccg agttcatctg atgaaaccga
     2641 tgttggacat ttggcttcag cttcgagcaa gtcgtcagca atttgactat acacttcttc
     2701 aggtgtcgat tgtgtcaggt ttttataggc atcaacactt tcaaggttct tgttaataat
     2761 tggtgcccat ccgtaatgac gcactaaatc ccagtagaag tatgctctaa gaaatttggc
     2821 ttcaccaatc atacgatctt taagaccatt ggttttccat tgaatgcctt cggcacgttc
     2881 gataaaaaga tttgcacggt atatacctga ataacacctg ttccataggt ttttaggagc
     2941 atcgctctca agactgatat tgaaacgctc ttgttcttgc caatactcca tgtcgctacc
     3001 gctggcatct cctccggtat aggcatcgtc tgattttata tcagacataa ttggaacaaa
     3061 actccagttt tgaaccgata gggcatcata tactgcagtg atggccaaaa atgcatcatt
     3121 ttcggttatg taggcattcg attctaactt cgatacttta ggagtaagtt cgataaaatc
     3181 tgtggtgcag gcctgaaaga ttgcgatcag gctcaatgct ataattaact ttttcatttt
     3241 cataatcttt aagtaaaagt tagaaagtaa tattcatacc aaaagtgatt gtttttggtt
     3301 gagggtatac cccgcggtcg actccggcac caaaagtatt gttgctacca atttcaggat
     3361 cgaaccctgg gtaatttgtt aaggtaatga ggttttctcc catcacgtag aaccttagat
     3421 tttcgatttt tgctttctgt gtcaatgatt tcgggaaagt ataacccaca gtaaggctcc
     3481 ttaaacggcc aaaacttgag tcgtaaacaa gcaagtcgct aggtgttttc cagttgccat
     3541 tgaaattcaa atcgtttttt gctaaacgcg ggtatttgtt cgaggtgcct tcacctgtcc
     3601 atcttccgtc gtacatttct ttggtgtagt tggttgtcca ttggtcgtag cggcgcagta
     3661 ccatccatgt ttcctgtcct aaagcaacat accagaacat gttaaagtcg atgcctttcc
     3721 agtcgagtga aaggtttaag cctccggtaa attcaggcat ggcattccca aggtttaccc
     3781 tgtcgaggtc ggtaagcaag ccatctttgt tagtgtcaac aaaacgaacg tcaccaggtt
     3841 tagcatttgg ctgaagtttg aaagtgcggc ccaagctatc agttgtggtg tggttggcaa
     3901 catcatcggc tgtttgaaag agaccatcgg ttttgtagcc ccagaagaat gctgcgggtt
     3961 gccccacttc gaagcgaacg atatttccct gaccaaaacc tccagaacca cctactataa
     4021 ctttttctgt gttaggaatg tcaataactt cattccggtt gaatgcgcca gttaccttta
     4081 catcaatatt aacatttccg gctcttgctt tgtatcccat ttcaagttct aaccctttgt
     4141 tgtttactga accgccatta attgttggcg ctgagtttcc aacgagcata ggaacaggag
     4201 ctgttattag ccagtct
//
LOCUS       DAOPUW010000082         4194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_21373_length_4194_cov_3.130466, whole genome shotgun sequence.
ACCESSION   DAOPUW010000082 DAOPUW010000000
VERSION     DAOPUW010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21373_length_4194_cov_3.130466"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1366)
                     /locus_tag="PKJ43_02250"
     CDS             complement(86..1366)
                     /locus_tag="PKJ43_02250"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNZ71411.1"
                     /translation="MNGNKVDVLLGLQWGDEGKGKVVDVLTPGYDIIARFQGGPNAGH
                     TLEFNGIKQVLHTIPSGIFREDKMNIIGNGVVLDPAIFKKEIESISRIGFDLSEKLLI
                     SKKAHLILPTHRLLDAASESAKGDTKIGSTLKGIGPTYRDKIGREGLRVGDICENFEE
                     KYNAHKKQHLLQLDNFYHYNYSDQLAQFEKEWFEGIEVLKKFKFIDSENYLNNALKAG
                     KTVIAEGAQGTMLDIDFGSYPFVTSSNTICAGACTGMGLAPQRIGNVYGIFKAYCTRV
                     GMGPFPTELFDEIGQQLRDRGNEYGSTTGRPRRCGWLDLVALNYSIMINGVTDLIMMK
                     ADVMDEFDTIKICTAYKINGKETTEFPYELNENVEPVYTELPGWKTDLTELTSEEQFP
                     IKLKEYIKFIEEQTGVSISIVSVGPNRKQTIVRK"
     gene            complement(1739..2161)
                     /locus_tag="PKJ43_02255"
     CDS             complement(1739..2161)
                     /locus_tag="PKJ43_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNZ71412.1"
                     /translation="MKATAHQLFTDYLEKNAHRKTPERYAILEEISSREGHFDVESLY
                     ISMKNKKYRVSRATLYNTIELLLDCKLLVKHQFGKNIAQFEKAFATRAHDHLIDLSTG
                     TVSEFFDQRIRQIIEDACKDNNFKLSHHTLYIYGERIK"
     gene            complement(2231..>4194)
                     /locus_tag="PKJ43_02260"
     CDS             complement(2231..>4194)
                     /locus_tag="PKJ43_02260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HNZ71413.1"
                     /translation="HDVVEDTEYTLDDIRTLFGDKIATIVDGLTKLEGDFDYKQAANF
                     RKMLLTLSDDVRVILIKIADRLHNMRTLDSMLPNKQLKISGETLYIYAPLAHRLGLYA
                     IKTELEDLSLKYKYPEQYREISLQLRDKEEHRLYLVNQLIKPIEEKLKKENLEFSIFG
                     RPKSVYSIWKKMQVKKVSFDEVYDLLAIRIIFKADEHMDERRKCWEIFSVVTSTYNYK
                     QDRIRDWITMPKANGYEALHLTVMGPEGKWVEVQIRSERMNEIAERGFAAHWKYKTGS
                     SHESELDKWIERIKEMLQNPESDVLEFLDDFKLNLFASEIVVFTPNGEMKTLPKKATV
                     LDFAYEIHSDLGNKCIGAKINHQLVPISYELRSGDQIEILTSEKQKPQAHWFDIVTTA
                     KAKQKVKDSFKLEHNQHIQLGKQLIEERLKAIDKIPSNETINQLLTHFNLDSKIQMYA
                     QVGSGLSSLNNIETIFEEKKEANKLIKYWRLSLGRKKKSTSEPENKGLPIDKRKPYVL
                     SEENLEKNFTLAKCCNPIPGDDVIGYIDNNNKVIIHKKSCPETVRIMTSEGDKIVTAT
                     WKQFKIQSYLSSIIIKGFDRVGILSEIAGIISKDHNVNMRTVHFDTNDGVFTGSIDVY
                     IHNTEDLDDIISKMTKIKGIEIVERIEKS"
BASE COUNT         1084 a          799 c          799 g         1512 t
ORIGIN      
        1 taaacttatc gttaataaac aatagcaaac cgatgaaaac aaaaaatccc ggaaataccg
       61 ggattcgaag tatatttcag tgaacttatt ttctgacaat tgtttgtttt cggtttggcc
      121 cgactgaaac tattgaaata gatacgccag tttgttcttc aatgaacttg atgtattctt
      181 tcagcttaat cggaaattgt tcttctgagg tcaactcggt taaatcggtt ttccagccag
      241 gaagttcagt atatactggt tcaacgtttt cattgagttc gtatggaaac tcagtggttt
      301 ctttcccgtt gattttgtat gcagtacata tttttatcgt gtcaaattcg tccattacgt
      361 cggctttcat cataatcagg tctgttactc catttatcat gatagagtag ttcagagcaa
      421 ccagatcgag ccatccgcaa cgtctgggcc ggccggttgt ggagccgtat tcatttcctc
      481 ggtcgcgcaa ttgttgtcct atttcatcga aaagttctgt tgggaaaggt cccatgccaa
      541 cacgtgtgca atatgctttg aaaatgccat atacattgcc gattcgttgt ggggcaagcc
      601 ccattcctgt acaagctccg gcacaaattg tgttcgacga agttacaaat ggatatgaac
      661 caaaatcaat atccagcatg gttccttgag ctccttctgc aataacagtt tttccggctt
      721 tcagtgcatt gttcagatag ttttcactat caatgaattt gaatttcttc aatacttcaa
      781 tgccttcaaa ccattctttt tcaaactgag ccagttggtc tgagtagtta taatggtaaa
      841 aattatcgag ctgtaaaaga tgctgttttt tatgagcatt gtatttttct tcgaaatttt
      901 cgcagatatc gccaacacgc aagccttcgc gtccaatttt atcacggtaa gtagggccaa
      961 tgcctttcag cgtagaacca atctttgtgt ctcctttagc agattccgag gctgcatcga
     1021 gcagtcgatg ggttggaagg atcaaatggg cttttttcga tataagcagc ttttcagaaa
     1081 gatcaaatcc tatgcggctg atcgattcta tttctttttt gaatattgcg ggatccagca
     1141 caacaccgtt gccgatgatg ttcattttgt cttctctgaa aattccagaa ggaatggtgt
     1201 ggagtacttg tttaattcca ttaaactcaa gggtgtggcc ggcattgggg cctccctgaa
     1261 agcgggcaat tatatcgtac ccaggggtta atacatcaac aactttacct tttccttcat
     1321 cgccccattg gagcccaaga agtacatcaa ctttattacc gttcattgtt attcttatgt
     1381 ctaaagtatt attcttcaag ttctgtatag taagtttacc ttacaccaaa acaatattga
     1441 agctcataaa aataggctta ttctatactt ctgatagaac aaaccggcca attacttcgg
     1501 taactgtttt ggctttcaaa tttataaaac aattgaacca atgcaaacta tttaggcttc
     1561 atatgttatt cttgagccaa aagttattgt ttgggtagta tatgaattga ttttttactt
     1621 ggttgcttaa tatttgtaac ataacacaat agataactgc ttcgtattta aaagtttacg
     1681 ttagatcttt ttaaatggta actgctattt attgagaaaa ttggtatccg ggtttcactt
     1741 attttattct ttccccataa atatatagtg tgtggtgaga gagtttgaaa ttattgtctt
     1801 tacacgcgtc ttcaattatt tgtcgtatac gttggtcgaa aaactcactg actgtccctg
     1861 tcgataagtc gataaggtgg tcgtgtgccc gggtagcaaa ggctttctca aattgagcaa
     1921 tgtttttacc aaattgatgt tttacaagaa gtttgcagtc aagcaataat tctattgtat
     1981 tgtataaggt tgctctgctt acccggtatt tcttgttctt cattgaaatg taaagtgatt
     2041 ccacgtcaaa gtgtccttca cgactgctta tttcttctaa tattgcatac ctctcaggag
     2101 tcttccggtg tgcatttttt tccagataat cagtgaatag ctggtgagcc gtagctttca
     2161 tattttgatt attcataatt aaactaatta taaattggaa caaatatata aaaacccttt
     2221 gaatttctat tcagcttttc tcaattctct caacaatctc tattcctttg atttttgtca
     2281 tttttgatat tatgtcatct aagtcttcag tattgtgtat gtacacatca atactgccag
     2341 taaagactcc gtcattggtg tcgaaatgga cggttcgcat gtttacattg tgatcttttg
     2401 aaatgatacc cgcaatttca ctaaggattc caacccggtc gaagcctttg attattatgc
     2461 tgctcaggta cgattgaatt ttaaattgtt tccaggtagc ggtaacaatc ttgtcacctt
     2521 cgctggtcat aattcttacc gtttctgggc acgacttttt gtgaatgatt actttattat
     2581 tgttgtcgat atacccaatt acgtcgtcgc cgggaatagg gttacagcat ttcgccagcg
     2641 tgaaattctt ctctaagttt tcttcgctga gcacataggg ttttcttttg tcaatgggaa
     2701 gtcctttgtt ttcaggttcc gatgtgcttt ttttctttcg gcctaagctt aaacgccaat
     2761 atttaattag tttgttggct tcttttttct cttcaaaaat tgtttcaatg ttgttgagcg
     2821 aactgagtcc tgatccgact tgtgcataca tttgaatttt agaatcgagg ttgaagtggg
     2881 tgaggagctg gttaattgtc tcgttcgaag gtattttgtc tattgcttta agtcgctctt
     2941 caatgagttg cttgccaagt tgaatgtgtt gattatgttc gagtttgaaa gaatctttaa
     3001 ctttttgttt tgctttggca gtggttacta tatcgaacca atgagcctga ggtttttgtt
     3061 tttctgaggt taatatttct atttggtccc cactacgcaa ttcgtagctg attggcacta
     3121 attggtggtt aatttttgca ccaatacatt tatttccaag gtcagaatgt atttcataag
     3181 caaaatcaag aactgtggct ttctttggta aggttttcat ttcaccattt ggggtgaaga
     3241 ctacaatttc tgaagcaaaa aggtttagct tgaaatcatc gagaaattcg agcacgtcgg
     3301 attccggatt ttgaagcatt tcttttattc gttctatcca cttgtcgagt tcgctttcgt
     3361 gcgacgagcc tgttttgtat ttccagtgcg cggcaaagcc cctttctgca atttcgttca
     3421 ttcgttcgct gcggatctgt acttcaaccc attttccttc tggccccatg actgttaggt
     3481 gaagtgcttc ataaccattg gctttaggca tggtaatcca gtcgcgtatc cggtcttgct
     3541 tataattata ggtcgatgtc actaccgaga atatctccca acatttcctc cgttcgtcca
     3601 tgtgttcatc agccttaaat attatcctaa tggctaataa atcatacact tcgtcgaatg
     3661 aaactttttt cacctgcatc tttttccata tggaatacac cgattttggg cgaccaaaaa
     3721 tcgaaaattc aagattttct ttctttagct tctcttcaat tggtttaata agctgattta
     3781 caagatataa tcgatgttct tccttgtcgc gcagttgtaa tgaaatttca cggtattgtt
     3841 cagggtattt atattttaag cttaagtctt ctagttctgt tttaatggca tacagtccaa
     3901 gccgatgagc caaaggagca tatatgtata gtgtttcacc tgatattttc agttgtttgt
     3961 ttggaagcat cgaatcgagc gtacgcatgt tatgaagccg gtcagcgatc ttaatcagaa
     4021 ttactcgtac atcgtcagaa agtgttagta acatttttct gaagttggca gcttgcttgt
     4081 agtcaaaatc gccttcgagt tttgttaatc cgtcgacaat agtggcaatt ttatcaccaa
     4141 ataatgtgcg tatgtcatcg agtgtatatt ctgtatcttc aacaacatca tgta
//
LOCUS       DAOPUW010000083         4181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_21448_length_4181_cov_3.825012, whole genome shotgun sequence.
ACCESSION   DAOPUW010000083 DAOPUW010000000
VERSION     DAOPUW010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21448_length_4181_cov_3.825012"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PKJ43_02265"
     CDS             complement(<1..418)
                     /locus_tag="PKJ43_02265"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNZ71414.1"
                     /translation="MKLFQRSLKSVFSIRYKKIEQFCKDPKGNQEAVLKELLAKAKKT
                     EWGTSHNFKKVKSYEEYTANVPLNDYETLKGYIERMVSGEKNILWPGQVKWFAKSSGT
                     TNDKSKFIPVSNESLHKCHYKAALDVFALYVKNNPDT"
     gene            531..1085
                     /locus_tag="PKJ43_02270"
     CDS             531..1085
                     /locus_tag="PKJ43_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNZ71415.1"
                     /translation="MNYIDYIVIILLLFSFLGGLKNGFIYELASLVGLVAGVWGAIKF
                     SGATRDFLEFRLNFTGDWVQILAFGLTFAAIAILVHIIAKAIEHALEMVNMGALNRIF
                     GGLLALFKSAFFFGLFFMFIDTIDEMLPVFPEKKIKEAKMYEPMRDVAIFSFPFFKGV
                     YEDFKGPDRNKEKKNAEPDSLKKS"
     gene            complement(1080..1796)
                     /locus_tag="PKJ43_02275"
     CDS             complement(1080..1796)
                     /locus_tag="PKJ43_02275"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNZ71416.1"
                     /translation="MAHNNYFEFKQFTVKQNNAAMKVGTDGVLLGAWTNVENAKHILD
                     IGTGTGVVALMLAQRSQANIVAIDCESSACIDARLNVLNSPWSQRIAVQEICLQEFAA
                     TTTQQFDCIVCNPPFFSNAVKPNDVKRSMARHNHSLPFDELLKSAALLLTPNGHFTVV
                     LPANAENSFRMLASNYRLFPSRITRIKPKPSASISRVLLEFRNEATLNLETELTIETE
                     KHHEYQPETIALLRDFYLSL"
     gene            complement(1796..2563)
                     /locus_tag="PKJ43_02280"
     CDS             complement(1796..2563)
                     /locus_tag="PKJ43_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HNZ71417.1"
                     /translation="MEKSELKAFLDEKFYEYNQPAFIENDPVQIPHLFDKKEDIEIAG
                     LLTATIAWGNRASILKNSQRLMKEMGYFPHEFIMNASKNELTAFENFVHRTFNGIDAI
                     FFIQSLKNIYENHNGLEAVFSKGYENEHSIAGALAYFRSVFLQVPHLKRSEKHVSNVE
                     KNASCKRLCMFLRWMIRDDNRGVDFGLWKKIPTSALMLPLDIHTGNISRMLGLLERKQ
                     NDWKAVEEVTASLRSFDENDPVKYDFSLFGLGVNKAI"
     gene            complement(2629..3282)
                     /locus_tag="PKJ43_02285"
     CDS             complement(2629..3282)
                     /locus_tag="PKJ43_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ71418.1"
                     /translation="MIKAANITKSFGPLQVLKGVTLEAPKGKIISIVGASGAGKSTLL
                     QILGTLSLPDGGTLQIDNSNPLNMSEKELARFRNKHIGFVFQFHHLLPEFTALENVCL
                     PAFIAQTAKKDAEKRASELLTFLGLKDRLTHKPNELSGGEKQRVAVARALINKPSVIF
                     ADEPTGNLDSQNQQEMHDLFFSLRDEYKQTLIIVTHDRNFAKMSDVIVEIKDGVILK"
     gene            complement(3302..3547)
                     /locus_tag="PKJ43_02290"
     CDS             complement(3302..3547)
                     /locus_tag="PKJ43_02290"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNZ71419.1"
                     /translation="MSGSEILVVVAAILLLFGAKSIPEFARFFSKGMRELKKATSEIQ
                     KEIEGYTADISDEISDIKSNLNKEIKNIDDEINPNSK"
     gene            complement(3696..>4181)
                     /locus_tag="PKJ43_02295"
     CDS             complement(3696..>4181)
                     /locus_tag="PKJ43_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71420.1"
                     /translation="NTGSEPLSYSSALHSYFNISGIANIRVAGLEGKTFYNGFESTTN
                     VQQEELLAIEKEENRRYVDVTNDCLIIDQPFNQITRVAKRGSNVTVVWNPGSETAAKM
                     DDITDEGWQEFICVEAVNAYNDIIHLSAGQSHTTATTISLDLKASNLRMSEQDSSFTV
                     I"
BASE COUNT         1078 a          814 c          885 g         1404 t
ORIGIN      
        1 ttgtgtcagg attatttttt acataaagag caaaaacatc gagcgctgct ttgtaatggc
       61 atttgtggag cgattcgtta ctcaccggga taaacttgct tttatcgtta gttgttcccg
      121 acgattttgc aaaccatttt acctgcccgg gccacaagat gttcttctcg cccgaaacca
      181 ttctttcaat atatccttta agggtttcat agtcgttcag cggaacattt gcagtatatt
      241 cttcatacga tttaaccttt ttaaaattgt ggctggtacc ccattcggtt ttctttgctt
      301 tagctagtaa ttctttcaaa acagcttcct gattaccttt ggggtcttta cagaactgtt
      361 ctatcttttt gtatcgtatg ctaaaaacac ttttaaggct ccgttgaaac aatttcatgt
      421 aatcagtttt tcggttcgta ataataggtg aaagtaacaa atttataggc taaaaatctg
      481 tttcttttaa tttggtgcgt taattttgtt caaattgtaa ttttttagcc atgaattata
      541 ttgactacat tgtaatcatt cttttactct tttcttttct tggaggatta aagaatggtt
      601 ttatttatga actagcttcg ttggttggct tggtggctgg cgtttggggg gcaataaagt
      661 tttctggcgc tacccgcgat tttcttgaat tccgcttgaa ctttaccgga gattgggtac
      721 aaattttggc cttcgggctc acttttgctg ctatagctat cttggtacat attattgcca
      781 aagcgattga acatgcactc gagatggtga acatgggggc actcaatcga atttttggtg
      841 gactattagc cttgttcaaa agtgctttct tttttggact tttttttatg ttcatcgaca
      901 ccattgatga gatgttgcct gtttttcccg agaaaaagat aaaagaagcc aaaatgtatg
      961 agcccatgcg agatgtggct atttttagtt ttccattttt taaaggagtc tacgaggatt
     1021 ttaaaggtcc cgacagaaat aaagagaaga agaatgctga gcccgactca ctgaaaaaat
     1081 cataacgata aataaaaatc gcgtagtaaa gctatcgttt caggctgata ttcatgatgc
     1141 ttttcggttt caatggtcaa ctcggtttcg agattaagag tagcttcatt cctaaattct
     1201 aaaagaaccc ttgatatgct ggctgagggt tttggcttta tccttgtaat gcgcgatgga
     1261 aaaagcctgt aatttgaggc gagcatcctg aaactgtttt cagcattggc agggagtacc
     1321 actgtaaagt ggccgtttgg cgtaagcaaa agtgctgcac tttttagcag ttcgtcgaaa
     1381 gggaggctgt ggttgtgccg ggccatagaa cgtttcacat cgttaggttt gaccgcattg
     1441 ctgaagaagg gagggttaca aacgatacaa tcgaattgtt gcgttgtagt tgccgcaaat
     1501 tcttgtaaac agatttcttg taccgcaata cgttggctcc atggcgaatt caacacgttt
     1561 aggcgtgcat cgatacaggc agaagattca caatcgattg caacaatatt tgcctggctc
     1621 cgttgggcaa gcatcagcgc aaccactcct gttcctgttc caatatcaag gatatgtttt
     1681 gcgttttcaa cgttggtcca ggcgccaaga agaaccccgt cggtgccaac tttcattgct
     1741 gcattattct gtttaacagt gaattgtttg aactcgaaat agttattgtg tgccatcaga
     1801 ttgctttatt taccccaagg ccgaagagtg aaaaatcgta ttttaccggg tcattttcat
     1861 caaaacttct gagcgaagcg gttacttctt caacagcttt ccagtcgttt tgttttcgct
     1921 ccagtaaacc aagcattcgt gaaatatttc cggtatggat gtcgaggggc agcattaatg
     1981 ccgatgttgg gatttttttc cataatccga aatctactcc acgattatcg tcgcgaatca
     2041 tccagcgaag aaacatgcag agtcttttgc acgatgcatt tttttcaaca tttgacacgt
     2101 gtttttcgct tcgtttaaga tggggaacct gaaggaaaac agaccggaaa tacgcaagag
     2161 caccggctat actatgttca ttttcatatc ctttgctgaa aacagcttca agcccgttgt
     2221 ggttttcgta tatgtttttc aacgattgaa taaagaaaat agcatcgatt ccgttgaatg
     2281 ttcggtgaac aaaattctcg aaagcagtaa gttcgttttt cgaggcgttc atgataaatt
     2341 catgcgggaa gtagcccatt tctttcatca ggcgttggct gtttttcaat atcgatgcgc
     2401 ggtttcccca ggcaatggta gctgtgagca aaccggcaat ttcgatatcc tcttttttgt
     2461 cgaaaaggtg aggaatctgc accgggtcgt tttcgatgaa tgctggttgg ttatattcgt
     2521 agaatttttc gtcgagaaaa gcttttaatt cgcttttttc catagtctta attgcttttc
     2581 aatgtaatag gcactgcaac aacggatgtg ctgaaactta cgatgagcct attttaaaat
     2641 taccccatct ttaatttcta caataacatc gctcattttg gcaaagttgc ggtcgtgggt
     2701 aacaattatg agagtttgtt tgtattcgtc gcgcaacgag aaaaacaaat cgtgcatttc
     2761 ctgttgattt tggctgtcga ggttgccggt gggttcgtcg gcaaaaataa ccgaaggttt
     2821 gttaatgaga gccctggcca ctgcaacgcg ttgcttttct cctcccgaaa gttcgtttgg
     2881 cttatgggtt aaccggtctt taagcccaag aaaagttagc agctcacttg ctcttttttc
     2941 ggcatctttt tttgctgttt gggcaataaa tgcaggcaaa catacatttt cgagggcggt
     3001 gaattcgggc agcaaatgat gaaattgaaa aacaaaccca atatgtttgt tgcgaaaacg
     3061 ggcaagttct ttttcgctca tgttcagagg attgctattg tctatctgca aagttccacc
     3121 atcggggagg ctgagcgtac caagaatttg caatagggtc gatttgcctg caccgcttgc
     3181 tcctacaatg gaaataatct tcccttttgg agcttctagg gtgacccctt tcaacacctg
     3241 caggggaccg aacgatttgg ttatgttagc tgcttttatc aatgctcgtt gaatttattt
     3301 actatttgct gtttggattt atttcgtcgt cgatgttttt aatttctttg ttcaggttac
     3361 ttttaatatc cgatatttcg tccgaaatat cagcggtata cccttcaatt tctttttgaa
     3421 tttcgctggt tgctttcttt aattcacgca tccctttgct aaagaaacga gcaaattcag
     3481 ggattgattt tgccccgaac aaaagaagaa ttgctgcaac gacaactaaa atttctgatc
     3541 cactcatgtt ttttgtgcca aattcgagtc caaatttagc tgaaatatta caaatgttat
     3601 actcaatgcc tcattattgc attctgtaag caaatcaaaa agagtgacgg attcaattcc
     3661 atcactctct ttttaaaact attatcgatt gcaaattaaa ttactgtaaa gctgctgtct
     3721 tgttcgctca tacgtaagtt gctggctttt aagtcgaggc tgattgttgt tgcagtagta
     3781 tgcgactgac cggcactgag atggattata tcgttgtagg cattaaccgc ttcaacgcaa
     3841 ataaattcct gccatccttc gtctgtgatg tcgtccattt ttgctgcggt ttcacttcct
     3901 gggttccata ccacggtaac attgctgccc cgtttggcaa cacgggtaat ttgattaaaa
     3961 ggttgatcaa ttataagaca atcatttgta acatctacat aacggcggtt ctcttctttt
     4021 tcaatcgcca gcagttcttc ttgctgaacg ttggttgtgc tctcgaaccc attatagaat
     4081 gttttccctt cgagtccggc tacacgtatg ttggcaattc ctgaaatatt gaaatatgaa
     4141 tgaagtgcag aggaataaga aagtggttca gagccggtgt t
//
LOCUS       DAOPUW010000084         4154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_21622_length_4154_cov_3.010490, whole genome shotgun sequence.
ACCESSION   DAOPUW010000084 DAOPUW010000000
VERSION     DAOPUW010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21622_length_4154_cov_3.010490"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PKJ43_02300"
     CDS             complement(<1..404)
                     /locus_tag="PKJ43_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71421.1"
                     /translation="MERVPQVIIIDRNTLSCIALHSILSDIIPFADIRSYNSFADFSQ
                     ASHNMVFHFFVSEDTVLQNINYFLHIQEKVIVLTTTSITPFGLSSFKTIDISRSEKEI
                     IKSFLSLHNTGHQNGHKRIQEHNDKGNLLSER"
     gene            568..1347
                     /locus_tag="PKJ43_02305"
     CDS             568..1347
                     /locus_tag="PKJ43_02305"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNZ71422.1"
                     /translation="MSLLFAVLSVLFLAKSEPADTSKLLKTIDVVAPIKQNADARVQP
                     VSSTTLGLFNLEEMRIHSVMDISAISPNFYQPDYGSKMTSSIYIRGFGSRIDQPVLGL
                     NVDDLPCLNKNNYNFNFYDIRKVELLRGPQGTLYGRNTIGGVMNIYTLSPMVYQGVRL
                     SAEYGSANSIRAKASFYKKVSRKFGYSAGVFYAHTDGFFQNEFRNEKCDWGDDAGARI
                     RTSWHPTKKISVENIASVGYTNEGGYAYSMFDNQTNVLKPV"
     gene            1399..2808
                     /locus_tag="PKJ43_02310"
     CDS             1399..2808
                     /locus_tag="PKJ43_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ71423.1"
                     /translation="MKYSGDKFQFSSVTGYQFTNDRMSIDNDFTSESFFTIIQHQKEH
                     ALSQDFVLKSKEGKQWQWLAGAFGFMKHIDMDAPVTFKEDGIDELILENANSGIHTVF
                     EDADIMIEENEFPIESNFVVPTYGSALYGQVSRNVGQFTLQIGARLDYESSSMSYNNS
                     ALIHYRFTKTMPNYKALTTVFKGKERLSFLEFLPKASLLFNLTNGNVYFSAAKGYKAG
                     GFNTQIFSDILQSKMMNNLMSDLGVHLDGVGSTTYNTASATTYKPEYSWNFELGSHLM
                     LIDNCLQLDWSAFLINCNNQQLTVFPQGKSTGRMMSNAGKSRVVGGEVSALYKFKELT
                     VGVNYGHTNARFVEYNDGNNSYAGNFLPYAPQNTVSANAAYKIHLNKKVIDRIVGACA
                     WQAIGIIYWNEDNTLSQPIYNLLSASLSFQKSVFDLTLWGKNLTNESYNTFYFKSIGN
                     NFFSKGKPLQAGITLTINL"
     gene            2815..3276
                     /locus_tag="PKJ43_02315"
     CDS             2815..3276
                     /locus_tag="PKJ43_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71424.1"
                     /translation="MKKMKIVLLAALVVAAFVSCEKDEDQKEAVVEVVSETYVGSLSV
                     NQNDGTFFTLDSVSVEVNPTDGDSTLNMIMRGVKFSSRMPVSLDMTVNGIAYTSTTTN
                     VSLSGNNIIPIAMGGEFPDYTIINYVGTITDGVLSFSMVCGKYPLTFTSTK"
     assembly_gap    3589..3598
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3599..>4154)
                     /locus_tag="PKJ43_02320"
     CDS             complement(<3599..>4154)
                     /locus_tag="PKJ43_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HNZ71425.1"
                     /translation="NQVWMVENLKTTKYNDGTEILNVTSYTQWDNLTTGAYCNYDNLE
                     SNAATYGRLYNWYAVNTGKLAPAGWHVPTDDDWTILENYLIANGYNYDGTLDEDKIAK
                     SLCAKTNWALSSEDGTPGAAPENNNSTGFTALPGGYRHYDGDFYSIGEGGYWWSSTEY
                     DESSAYRRGLYYRKGGFSRADNYEV"
BASE COUNT         1170 a          845 c          910 g         1219 t
ORIGIN      
        1 tccctttccg atagaaggtt gcctttgtcg ttatgttcct gaattcgttt gtggccattt
       61 tgatgccccg tgttgtgcaa actcaggaaa gattttatta tctctttttc cgaacgggaa
      121 atatcaattg ttttaaacga cgacaaccca aacggtgtga tggatgtggt tgttagtaca
      181 ataaccttct cttgaatatg aaggaagtag ttaatgtttt gcagtactgt gtcttctgaa
      241 acaaaaaagt gaaacaccat attgtgcgat gcttggctga aatcggcaaa agagttataa
      301 ctgcggatgt cggcaaaagg tattatatcg gaaagaatag aatgcagcgc aatacaactc
      361 agtgtgttgc gatcaataat tataacttgc ggaactcttt ccatttcagc cgcaatatta
      421 acacctttcg aggtaaaata aaaaccctca caaatgaggg ttgacagcca ttttgaagct
      481 tttacttttg cggtgttatc aattgcgata ctacttaatt tatttcgatc tcaaaattcg
      541 taatgcaaca accttaaaat tagtggagtg agtttactat ttgccgtatt atctgtttta
      601 tttttagcta aatcggagcc tgctgataca tctaaactgc ttaaaactat tgatgttgtt
      661 gcgccaatta agcagaacgc agatgcacgg gttcagccgg tttcttctac tacacttggg
      721 ttgtttaacc tcgaagagat gcgcattcat tcggtgatgg atatttcggc catttcgcca
      781 aacttttacc agcccgatta tggttcgaaa atgacttcct ctatttatat caggggtttt
      841 gggtcgcgaa ttgaccagcc tgttcttggc ttaaatgtcg acgatttgcc ttgcctcaat
      901 aaaaacaatt acaactttaa cttttacgat atccgtaagg ttgagttgct gcgagggcct
      961 caaggtacgt tgtatggtcg caacaccatt ggcggggtaa tgaatattta caccctttcg
     1021 cctatggttt atcagggtgt gaggttgtcg gccgaatatg gttcggcaaa ctcgataagg
     1081 gctaaagctt cgttttacaa gaaggtgtcg agaaaatttg gctattcagc aggcgtgttt
     1141 tatgcccata ccgatggttt ttttcagaat gagttccgaa atgaaaagtg cgactggggc
     1201 gatgatgcag gcgcacgcat tcgcacgtcg tggcatccaa ctaaaaaaat aagtgtagag
     1261 aacatcgcat cggtaggata tacaaacgaa ggtggttatg cttattcaat gttcgacaac
     1321 caaacaaatg tgttaaagcc tgtttaatac aatgatcggt gcgaatacaa acggataaac
     1381 atcagcgaag gtattgttgt gaaatacagt ggcgacaaat tccagttttc gagcgttaca
     1441 ggctatcagt ttacaaacga ccgaatgtct atcgataatg attttacttc ggaatcgttt
     1501 ttcactatca tacaacatca aaaggaacat gcacttagtc aggattttgt tttaaaatcg
     1561 aaagagggta agcagtggca gtggctggcc ggggcgtttg gctttatgaa acacatcgat
     1621 atggatgctc cggtaacgtt caaagaagat ggtattgatg aactgatcct cgaaaacgcc
     1681 aatagtggga ttcacactgt ttttgaagat gccgatatca tgattgaaga gaatgaattc
     1741 ccgattgaga gtaattttgt ggttccaact tacggctctg ctctttatgg acaagtttcg
     1801 cgcaatgttg ggcagtttac attgcaaatt ggagcccgcc tcgattatga atcttcatca
     1861 atgagttaca ataattcagc attaatccac tatcggttca ccaaaacaat gcccaattat
     1921 aaagcactga ctactgtttt caaagggaaa gaacgccttt cgtttcttga gtttttgccc
     1981 aaagcttcgt tgttgtttaa cctgacgaac gggaatgtct acttttcggc ggccaagggt
     2041 tacaaggctg gtggcttcaa tacccaaatt ttctccgaca tattacaaag taagatgatg
     2101 aacaacctca tgtccgatct tggtgttcat ctcgatggtg ttggtagtac aacttacaat
     2161 accgcatcgg caacaacata caagcccgaa tatagttgga actttgaact tggcagccat
     2221 ttgatgctta tcgataactg cttacaactc gattggtcgg catttctgat caactgcaat
     2281 aatcagcagt taaccgtttt ccctcaaggc aaaagcacag gccgtatgat gtcgaatgct
     2341 gggaagtcga gggttgtggg gggcgaggtg tcggctctgt acaagtttaa agaactgact
     2401 gttggcgtta attatggaca tacaaatgcc agatttgttg agtacaacga tggaaacaac
     2461 agttatgcgg gcaattttct cccttatgct ccgcaaaata ccgtgtcggc aaatgctgcg
     2521 tataaaattc acctcaacaa aaaggtaatc gatcgtattg ttggcgcgtg tgcttggcag
     2581 gccattggca ttatttattg gaacgaggat aacaccttgt cgcagccaat atataacttg
     2641 ttgtcggcat cgctctcttt tcagaagagc gtgttcgacc ttactttatg gggaaaaaac
     2701 cttaccaacg aatcgtacaa taccttttat tttaaatcga ttggcaataa tttctttagt
     2761 aaagggaaac cattgcaggc aggaataaca ttaactataa atttataaat actaatgaag
     2821 aagatgaaaa ttgtgctttt agcagcactt gttgttgcgg cttttgtaag ctgcgaaaaa
     2881 gatgaagatc agaaagaagc ggtagttgaa gttgtaagcg aaacttatgt tggatcattg
     2941 agcgtaaacc aaaatgatgg aactttcttc acgctcgatt cggtttcggt tgaagtgaac
     3001 ccaaccgatg gcgattcgac attgaacatg attatgcgcg gggtgaagtt ttcttcacgc
     3061 atgcctgttt cgctcgatat gacagttaat ggaattgcat acacatcaac aacaaccaac
     3121 gttagcttaa gtggcaacaa tattattccg attgctatgg gtggcgagtt tcccgattat
     3181 acaattatca actatgttgg cactataacc gacggggtgt tgtcgttctc gatggtttgt
     3241 ggcaaatatc cacttacatt tacaagtacc aaatagattt gatatttaaa tacactgttc
     3301 tccgataaat taaaaataag aaaccatttg cgatatgaga tccctctctc cactttgttg
     3361 cgttcgggat aacaggcact tacgttgacc aggagggggt tggttgcggc ttcgcggcca
     3421 ccaaccccct cctatataaa aaaccatgaa tgccccgtca tttcgaccga agggagaaat
     3481 ctcgtaagct ttaccttaca acagtgtaat caaatagtca cccttcgaca agctcagggt
     3541 aaaaatgaat caaataaatt aatcccttac caaccgaaca gacaacccnn nnnnnnnnac
     3601 ctcgtaattg tcggccctgg aaaagcctcc cttacggtaa tacaggcccc ggcggtaggc
     3661 gctgctctca tcgtactcgg tagaactcca ccaatagccg ccctctccaa tggagtagaa
     3721 atctccatcg tagtgacggt agccacccgg aagggctgta aaacccgtgc tgttattgtt
     3781 ttctggcgct gcaccggggg tgccgtcttc gctcgataat gcccagttgg ttttagcaca
     3841 aagcgatttg gcaatcttgt cttcatctaa agtaccatcg tagttgtacc cattagcaat
     3901 caaatagttt tccagaattg tccagtcatc gtcggtaggt acgtgccaac ctgccggggc
     3961 aagcttgccg gtgtttaccg cataccagtt gtaaagcctc ccataggtag cagcattgct
     4021 ttcgaggttg tcgtaattgc agtaggctcc agttgttaaa ttatcccact gtgtatagct
     4081 ggtaacattt aatatttcgg taccatcatt gtacttggtg gttttcaggt tttcaaccat
     4141 ccaaacctgg ttgc
//
LOCUS       DAOPUW010000085         4150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_21649_length_4150_cov_3.463492, whole genome shotgun sequence.
ACCESSION   DAOPUW010000085 DAOPUW010000000
VERSION     DAOPUW010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21649_length_4150_cov_3.463492"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PKJ43_02325"
     CDS             complement(<1..303)
                     /locus_tag="PKJ43_02325"
                     /inference="COORDINATES: protein motif:HMM:NF022430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2797 domain-containing protein"
                     /protein_id="HNZ71426.1"
                     /translation="MKFSGNLIKMRSEYTNPVTYFLQLGNSELKMNDLLGKSLLLKCS
                     GAIHCIQCGKRTSKSYSQGYCYNCMLTAPQADESVLRPELSKAQFGIARDMEWSKEN"
     gene            complement(442..3399)
                     /gene="secDF"
                     /locus_tag="PKJ43_02330"
     CDS             complement(442..3399)
                     /gene="secDF"
                     /locus_tag="PKJ43_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010421993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HNZ71427.1"
                     /translation="MQNKGAIRLFAIALALVCLYQLSFTYVSSRVQKKAVEIARGDEL
                     KEVQYLDSISSETVYNFLGIRKYTYKEVKQREINLGLDLKGGMNVTLEISVVDLIKSM
                     SNYSKDETFNKAIQLAAEKQKNSQDDFVTLFGQAYKEVDPNARLASIFLTPELKDQIS
                     FNSTNEQVLEVIRKQADAAIDNSFNILRSRIDRFGVVSPNIQKLNQRGRILVELPGVK
                     DKERVRNLLQGTAALEFWETLENQEVYSILLQVNDIVKNLNQSKSSASTTATDSVAGG
                     EKSLLDELESSKADAEKAGDNASDFPLFSALIPNANQQGQLFPGPVVGFSHFGDTSTV
                     NKYLNLPQVKLILPRDIKFAWQLKPFDEKGSLYQLVALKITTRDGRAPLTGSVITDAR
                     QDFGQSQASAEVNMNMDSEGAKTWARMTRDNVGRSIAIVLDGYVVSYPTVQTEITGGR
                     SNITGNFTVEEAKDLANMLKSGAMPAPAKIVEEYVVGPTLGRESINSGLWSFLISFAL
                     VLGFMMLMYSAQAGNIANVALLVNLFFIMGVLASFGAVLTLPGIAGIVLTVGMAVDAN
                     VLIYERIQEEIRAGKGIKLAVNDGFKNALSAIIDGQLTTMITGIVLYIFGSGPIKGFA
                     TTLIIGIFTSIFTSIFISRLIFEYFLGKNMNIKIITKFALHILQATNFKFIKFRKVAY
                     TISGVILGISLLSIVVRGFDYGIDFKGGRTYVIEFPQNVRVADVAQVLTDEFGSAPEV
                     KTFGSDNTVRITTDYKIDENNPSVDNEIEEKLYSGLQSFSGSVSKTTFVQSFIKSSNK
                     VGPTISDDIKTGAVWAIVLSLVFIFIYIMIRFKNWRYSLGGIAALAHDALVVLGFWSI
                     FRGLLPFNMEVDQTFIAAILTVIGYSINDTVVIYDRIREYVGLHPKASLEENMDNAIN
                     HTLRRTLNTSLSVIVVLVAIILFGGASIRGFAVAMFVGCISGVYSTVFVATPITYDVQ
                     RYIDRKKGKELAK"
     gene            3623..>4150
                     /gene="elbB"
                     /locus_tag="PKJ43_02335"
     CDS             3623..>4150
                     /gene="elbB"
                     /locus_tag="PKJ43_02335"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HNZ71428.1"
                     /translation="MLIKKFAVVLSGCGVYDGSEIHEAVCTLLAIEKAGACYECFAPN
                     VNQYHVINHLTGQVVNQTRNVLEEAARIARGTIKELSRFDPTHFDALIFPGGFGAAKN
                     LSTFAFDGADCLILPEVENAIRNAYNTRIPIGALCIAPTLLAKVLGKGNLTIGNDSDT
                     AAAIETMGSMHTNTLP"
BASE COUNT         1257 a          899 c          798 g         1196 t
ORIGIN      
        1 attctctttc gaccattcca tatcacgggc aattccgaat tgagctttcg acaactcggg
       61 acgcagcaca ctctcgtcgg cttgaggggc ggtgagcata caattataac aatatccctg
      121 gctatatgac ttagatgtac gctttccaca ctggatacaa tgaattgctc cactgcattt
      181 caacaaaagc gattttccca acaaatcgtt cattttcaat tctgaattac caagctgcaa
      241 aaaataagtc accgggttgg tgtactcgct ccgcatttta atcaggtttc ccgaaaattt
      301 cattgcatta actattataa aatagattac tcaaatgtaa ccataaaata cgttcatctt
      361 ctgttcaacg taggggttta atagcaagaa accctgctca agtattgagc agggtttcta
      421 tttatatatt ctaccaacga attacttggc caattctttg cctttttttc tgtcgatata
      481 gcgctgaaca tcgtatgtaa taggagtagc aacaaacact gttgaataaa cccccgaaat
      541 acaaccgaca aacatcgcta cggcaaaacc tcgaattgaa gctccaccaa agagtattat
      601 ggcaacaaga accactatta ccgaaagtga agtattcagt gtacggcgca aagtatgatt
      661 gattgcatta tccatgtttt cttcaagaga agctttagga tgcaagccta catactcacg
      721 gatacggtca tagataacca ccgtatcgtt gatcgaatac ccaattacag taagaatagc
      781 tgcaataaac gtttggtcaa cctccatatt gaaaggcagc agccccctaa aaatagacca
      841 gaatccgaga acaaccaaag catcgtgggc aagagcggca ataccaccca agctgtagcg
      901 ccagttttta aagcggatca tgatgtaaat aaagataaac accagagaaa ggactatcgc
      961 ccaaacagca ccagttttga tgtcgtcaga aatggtaggc ccaactttgt tcgagctctt
     1021 gatgaagctt tgcacgaatg tagttttaga tacgctacca gagaacgatt gcaacccact
     1081 gtatagtttt tcttctattt cattgtcaac cgatggatta ttttcatcaa tcttatagtc
     1141 agtagtaatg cgaacagtat tgtcgctacc aaaagttttc acttcaggag ctgatccaaa
     1201 ttcatcagta agtacttgag caacatcagc cactcgtaca ttctgtggga attcgatcac
     1261 ataagtacgt ccccccttga agtcgatacc gtagtcgaaa ccacgaacaa caatcgaaag
     1321 cagtgaaata ccaagaatta ctcctgaaat tgtataagca accttacgga atttgatgaa
     1381 tttaaagtta gttgcctgca atatgtgaag ggcaaacttg gtaataattt tgatgttcat
     1441 gtttttacca aggaaatatt cgaagatcaa acggcttata aaaatcgagg tgaaaattga
     1501 agtgaaaata ccgatgataa gtgttgtagc gaaccctttg attggacctg aaccaaaaat
     1561 gtaaagtaca attccggtaa tcattgttgt aagctgaccg tcgataatag ccgataaggc
     1621 gtttttgaac ccgtcgttca cagcaagttt tattccttta cccgctctaa tttcttcctg
     1681 tattcgctcg tatataagca cgttagcatc taccgccata cccacagtaa gcacaatacc
     1741 tgcaatacca ggcaaggtta atactgctcc aaacgaagct agaacaccca taatgaaaaa
     1801 caggttgact aacaaagcca cgttggcaat atttcctgct tgggcactat acataagcat
     1861 catgaagcct aatacaaggg caaaagaaat gaggaacgac caaagacctg aattgataga
     1921 ctcacgacca agggtaggac ctacaacata ttcttcaaca attttcgctg gagctggcat
     1981 tgcacccgac ttcaacatat ttgctaagtc ttttgcttct tcaacagtaa agtttccagt
     2041 aatatttgaa cggccaccgg ttatttcggt ttgaacagta ggatatgaaa cgacatagcc
     2101 gtcgagcacg atggcaattg agcggccaac attatcacgg gtcattcggg cccatgtttt
     2161 agcaccctct gaatccatgt tcatgttcac ctcagccgaa gcttgacttt gcccgaaatc
     2221 ctgacgggca tcagttatta cactaccagt taatggagca cgtccatcac gagtggtaat
     2281 ttttaacgcg accagttggt acaaagaacc cttttcgtcg aaaggtttca attgccatgc
     2341 aaattttata tcgcggggca aaatcaattt tacctgaggc aagttcaagt atttgtttac
     2401 cgttgaggta tcgccaaaat gtgaaaaacc taccacagga ccagggaaca attgtccttg
     2461 ctgatttgca tttggaatca atgccgaaaa caacggaaag tcggaagcat tgtcgcctgc
     2521 tttttcagca tctgcttttg aagactcaag ttcatcgaga agtgattttt cgccaccggc
     2581 aaccgaatcg gttgctgttg ttgaggcaga tgatttcgat tggtttaagt ttttaacaat
     2641 atcgttcacc tgaagaagaa tcgaatatac ttcctggttt tcgagagttt cccaaaattc
     2701 gagagctgcg gtaccttgca ataagttacg tacacgttct ttatctttta caccaggcaa
     2761 ctccaccaaa atacggccac gttgatttaa tttctggata ttaggagaaa ctacaccaaa
     2821 acggtcgata cgagaacgta aaatattgaa agagttatcg atagccgcat cagcttgttt
     2881 tcttattacc tcaagaactt gctcgttggt tgaattgaac gagatttgat cttttaactc
     2941 gggagtaaga aagattgatg ccaaacgggc attagggtca acctctttgt aggcttgtcc
     3001 aaacaaagtc acaaagtcgt cttggctatt tttttgtttt tcagcagcca actggatcgc
     3061 cttgttgaat gtttcgtctt tgctataatt agacatcgac ttgatgaggt caactaccga
     3121 aatttcgagg gtaacattca tacccccttt gaggtcaaga cccaagttta tttctctctg
     3181 ttttacctct ttgtaggtgt acttacgaat tcccagaaag ttatatacag tttcactgga
     3241 aatcgaatcg aggtattgta cttctttcaa ctcatcgcca cgggcaattt caacagcctt
     3301 cttctgaacc cggctcgaaa cataagtgaa cgacagctga tataaacata ctaaggctaa
     3361 agcaatggca aagagcctta tcgcaccttt attctgcatt tgtttttaaa ttttgttaat
     3421 tcttcttcaa gattttttta ctgaacggca aatatatctt ttttttaaaa accaactagt
     3481 atatgtagcg aagtaaatat cgatttgtga tttatctccc tatactttct aaaaggaagg
     3541 tttgaggtaa acctctatct gcctcttttt tttattttca ctgcttttta atatttttgg
     3601 atcaacagaa aatacgagca ttatgttgat aaaaaaattt gcagtagtat tgtccggatg
     3661 tggggtttac gacggctctg agattcatga agccgtgtgt accctattag ctattgagaa
     3721 agcaggggca tgttacgagt gtttcgcccc taacgttaac caataccacg taataaacca
     3781 ccttaccggg caagtggtta accaaacccg caatgttctt gaagaagcag cacgcattgc
     3841 ccgcgggacc ataaaagaat tatcaaggtt cgatccaacg catttcgatg cattaatttt
     3901 tcccggcggt tttggcgcgg caaaaaacct ttcgacattt gcctttgatg gggctgattg
     3961 cctgatactt cctgaagttg aaaatgctat tcgaaacgcc tataatacgc gcatacccat
     4021 tggggcactg tgcattgcac caacccttct tgccaaagtg cttggcaaag gcaatctaac
     4081 cattggtaac gattcagata ctgctgctgc cattgaaact atgggttcaa tgcataccaa
     4141 taccttgcct
//
LOCUS       DAOPUW010000086         4097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_22010_length_4097_cov_3.697674, whole genome shotgun sequence.
ACCESSION   DAOPUW010000086 DAOPUW010000000
VERSION     DAOPUW010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22010_length_4097_cov_3.697674"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PKJ43_02340"
     CDS             <1..391
                     /locus_tag="PKJ43_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNZ71429.1"
                     /translation="PYVVPMNFGYSDGFIYLHSAKEGRKWQLMKSNPKACVTFCLGDE
                     LAWQDEHIACSWRVKSKTVIAEGILEFVDDFEEKKNILKIFMAQYSDREFEFGAPAVR
                     NVGVIRMKIEVLSAKEFGAKAITPWNS"
     gene            400..1434
                     /locus_tag="PKJ43_02345"
     CDS             400..1434
                     /locus_tag="PKJ43_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNZ71430.1"
                     /translation="MMKKVLVTGATGMLGSRLVFDLFSLRVKVRAIYRNQARIDIFRR
                     YIKLYGGDPESVVEWVEWCEADVLNFHEMKDALEGVEMVYHCAAMVSFYPPDRSEMLR
                     INVNGTGNLVNACLETGVGSICHVSSIAALGKAEGDGPIDETAAWIPEEKHSGYSIAK
                     FHSEMEIWRGVEEGLKAVIVNPSVIIGPGDWKNGSSAFYGQLARGLRFYSSGVTGFVD
                     VRDVSAAMLLLTHEDNFLQACGNRFLLNAANLSYREFFTKIALSINAKVPTLPVTNIM
                     LSLAWRLAFLAGKITGNKPQITQETALSASKRATYNGSRISREYGFTYRPIDESIIEI
                     GKIYLADTNR"
     gene            complement(1679..3316)
                     /gene="groL"
                     /locus_tag="PKJ43_02350"
     CDS             complement(1679..3316)
                     /gene="groL"
                     /locus_tag="PKJ43_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456581.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNZ71431.1"
                     /translation="MMSKEIKFNIDARDSLKKGVDKLADAVKVTLGPKGRNVIIEKKF
                     GAPQITKDGVTVAKEIDLKDPYENMGAQMLKEVASKTGEDAGDGTTTATILAQSIITV
                     GLKNVTAGANPMDLKRGIDKAVVKVVENIKSQSQNVGDNYNKIEQVARISANGDETIG
                     KLIAEAMEKVNKEGVITVEESKSTDTYVDVVEGMQFDRGYISAYFVTNTEKMAAELDH
                     PYILIHDKKISTMKDLLPILEASAQTGRPLLIIAEDVDGEALATLVVNRLRGSLKVAA
                     VKAPGFGDRRKEMLEDIAILTGGTVITEEKGMKLEQATLDMLGRAEKVTVDKENTTIV
                     NGAGEQSNIAARVAQIKKQIEVTTSDYDKEKLQERLAKLAGGVAVLYVGAASEVEMKE
                     KKDRVDDALSATRAAVEEGIVPGGGVAYLRAIASLDGLTGDNEDETTGIEIVKRAIEE
                     PLRQIVTNSGKEGAVVVQKVREGKADFGYNARTDKYENLFETGVIDPAKVTRVALENA
                     ASIAGMFLTTECVLVDEKEDAPAMPAGGMGGGMPGMM"
     gene            complement(3343..3621)
                     /locus_tag="PKJ43_02355"
     CDS             complement(3343..3621)
                     /locus_tag="PKJ43_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNZ71432.1"
                     /translation="MADLKGKILAGKILVEPKAAEEKTASGIIIPDSAKEKPQQGVVV
                     MTGAALKDETMEVKAGDTVFYRKYAGTELHIDGTDYLLLSQSDVLYIA"
BASE COUNT         1034 a          826 c          928 g         1309 t
ORIGIN      
        1 gccctatgtt gtgccaatga attttgggta ttctgatggg tttatctatc tccactcagc
       61 caaagagggc aggaaatggc aacttatgaa gtcgaaccct aaagcttgtg taacattctg
      121 tctgggtgat gagttggcat ggcaagatga acatatcgct tgcagttggc gggtaaaatc
      181 gaaaacggta attgctgagg gaatccttga gtttgttgat gatttcgagg aaaagaaaaa
      241 catcctgaaa atatttatgg cacaatatag cgaccgtgag tttgagtttg gagctcctgc
      301 cgtgcgaaat gtgggggtaa ttcgaatgaa aattgaggta ttgtcggcaa aagaatttgg
      361 cgcgaaagca attaccccat ggaattcata agtgtttgaa tgatgaaaaa agtgttggtt
      421 accggagcca ccggaatgtt agggtcccga ttagtattcg atttatttag cctcagggta
      481 aaagtgaggg ctatttatcg aaatcaagcg cgtatcgata tatttcggcg gtatattaaa
      541 ctgtatggtg gtgatcctga gagcgtagtc gaatgggtgg aatggtgcga agccgacgtg
      601 cttaattttc atgaaatgaa agatgccctc gaaggggttg agatggttta ccattgcgcc
      661 gccatggtct cattttatcc gcccgacaga agtgaaatgc tccgtatcaa cgtaaatgga
      721 acaggaaacc ttgtaaatgc ttgccttgaa accggggttg gaagcatctg ccatgtaagc
      781 tcgattgctg cgcttggaaa ggccgagggc gatgggccaa ttgatgaaac ggcagcatgg
      841 attcccgaag aaaaacattc gggttacagt atcgcaaaat ttcactccga aatggaaata
      901 tggcgtgggg tagaagaggg cttgaaggct gtaattgtga acccttcggt aattattgga
      961 cctggtgatt ggaaaaacgg gagttcagca ttctatggtc agttggcgcg ggggttgcgc
     1021 ttttattcaa gtggggttac cggctttgtc gacgtgcgag atgtttcggc ggctatgcta
     1081 ttgttaaccc acgaagacaa ctttttgcaa gcctgcggaa atcgtttttt gctgaatgcc
     1141 gcaaacttat catatcgcga gtttttcacc aaaatagccc tttcgataaa tgctaaagtg
     1201 cccacgttgc ctgtaactaa tataatgcta tcgttggcct ggcgcctggc ttttcttgca
     1261 gggaaaatta cagggaataa gccgcaaatt actcaagaaa ctgcattaag tgcatcgaaa
     1321 agagctacat ataatggttc cagaatttct cgtgagtatg gattcacgta ccgacctatt
     1381 gacgagtcaa tcattgaaat tggaaagata tatcttgctg atactaatcg gtgagtattg
     1441 ttcggcaata aaagatttct caagagagtt gtttttcgca acaatacaat tttattgata
     1501 aagaaaaggt tgcctgccaa tttaaacaga caacctttta ggtatttatc gtggatgtga
     1561 tgttatccaa gagcaaattc aaaacaggcc aaaaccctca aatataaagc cgctgaatag
     1621 aaatgtcaag gctaatcggg tgagcgaaga ttggactgtt acatttcttc aatcagtatt
     1681 acatcattcc gggcataccg ccacccattc cgcctgctgg catagctggg gcatcttctt
     1741 tttcatcaac caaaacacat tcggtagtta agaacatgcc ggcaatagat gctgcatttt
     1801 cgagggcaac acgggtaact ttagctgggt cgataacgcc ggtttcaaaa aggttttcat
     1861 atttatcggt acgtgcattg tagccaaaat cagctttacc ttctcttact ttttgaacaa
     1921 caacagcgcc ttctttgccc gagttggtaa caatttgacg aagtggttct tcaatagcgc
     1981 gttttacaat ttcgatacca gtggtttcgt cttcgttatc gccagtgagg ccatcgagcg
     2041 aagcaatagc cctcaggtag gcaacgccac cgccgggaac aattccttct tctacagcag
     2101 cacgggttgc gcttaatgca tcgtcaacgc ggtctttctt ttctttcatt tcaacttcag
     2161 aagcagctcc aacgtaaaga acggcaacac caccagccaa tttggccagg cgttcctgca
     2221 gtttttcctt gtcgtagtct gaagtggtta cttcaatttg ttttttgatc tgggcaacac
     2281 gtgcggcaat attgctttgt tcgcctgctc cgtttacaat tgttgtattt tctttgtcaa
     2341 cagtaacttt ttcggcgcgg cctaacatgt cgagggttgc ttgttccagt ttcatacctt
     2401 tttcctcagt aatgacggtt cctccggtga ggatagcgat atcttcgagc atttctttgc
     2461 ggcgatcgcc aaagccagga gctttaacag cggccacttt taacgaacca cgtaagcggt
     2521 ttacaactaa agtcgccaat gcttcgccat caacatcttc agcaatgatc agcaatggac
     2581 ggccagtttg tgcacttgct tccaaaattg gaagaaggtc tttcatggtc gaaattttct
     2641 tatcgtggat taaaatataa ggatggtcga gttcggcggc cattttttca gtgttggtaa
     2701 caaagtaagc cgaaatgtat ccgcgatcga actgcatacc ttcaaccacg tctacatacg
     2761 tgtcggtgct cttcgattct tcaactgtga taacgccttc tttgttcact ttttccattg
     2821 cttcggcaat cagttttcca atggtttcgt cgccattggc tgaaatacgg gctacctgtt
     2881 caatcttatt gtagttgtcg cctacgtttt gcgattgtga tttaatgttt tctactactt
     2941 taacaacggc tttatcgatt ccgcgcttca gatccatcgg gttagctccg gcggtaacat
     3001 tttttagacc tacagtgatg attgactggg caagaatggt tgcagttgta gttccatcgc
     3061 cggcgtcttc tccggttttc gaggctactt ctttaagcat ttgtgccccc atgttttcgt
     3121 atgggtcttt aaggtcgatt tctttggcaa cagtaactcc gtctttggta atctgaggtg
     3181 cgccaaattt tttctcgata attacgttcc ttcctttggg gccgagtgtt acttttactg
     3241 catcggctaa tttatcgacg cctttcttta atgaatcgcg tgcatcaatg ttgaatttaa
     3301 tttctttcga cataatcaat tgttttaaaa aaggttaaga aattaagcaa tgtagagaac
     3361 atcactctga gaaagtaaaa gataatcagt accatcaatg tgtaattcgg tgccggcata
     3421 tttacgatag aatacagtgt cgcctgcttt tacttccatc gtttcgtctt tcaatgcagc
     3481 tccggtcatt acaactactc cttgctgggg cttttctttt gctgagtcgg gaataatgat
     3541 accacttgcc gttttttctt ctgcggcttt gggttctacc aggatttttc ctgctaaaat
     3601 tttacctttt aaatctgcca ttttttatga ttttaaagtt taacatgcat tttttattcg
     3661 tgcatgaggt ttcacagatt ttgtgccaat tgatttttct gccatttttt taccttattt
     3721 aatggcgcct ctgccaattc tcaggcatat ttgtctgttt ttgatgtgta agcattggat
     3781 aataagctgt ttagaggcaa gaaaagcctg tcagtgtgtc atactttggg cttttttgaa
     3841 gtgtcttgtt tttattaagc atgaaaagct tgtgtcgcaa gttgttaccg gcgcgagtat
     3901 ttttccttct tttatatgat tggttagaaa gaatgtttta gaaaaattat actcttcttg
     3961 tatgttgttt atggttctta ttactctatt gactcatcga ctttgggtca agatgctgat
     4021 cgaaccagtt gtcgatgacc gatcttctga agcgccattc tttgccgagt ttggctgcag
     4081 gtattttccc gttctgc
//
LOCUS       DAOPUW010000087         4061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_22266_length_4061_cov_3.545182, whole genome shotgun sequence.
ACCESSION   DAOPUW010000087 DAOPUW010000000
VERSION     DAOPUW010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22266_length_4061_cov_3.545182"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PKJ43_02360"
     CDS             complement(<1..662)
                     /locus_tag="PKJ43_02360"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNZ71433.1"
                     /translation="MKVIDILKKSEKTLFSFELLPPLKGSKGDKLYSTIDQLLEFDPK
                     YINITTHRDEYEFRTREDGLIEKRVVRKRPGTVAIAAAIQHKYGIPVVPHILCGGFSR
                     SETEHVLIDLDFLGIENVLALRGDGLKQESVFTPEKDGNKNANELVEQIASLGKGQYL
                     DPDLKNKASLDFCIGVAGYPEKHFEAPNLDTDLRYLKQKVDAGADYIVTQMFFDNQKY
                     FS"
     gene            complement(659..856)
                     /locus_tag="PKJ43_02365"
     CDS             complement(659..856)
                     /locus_tag="PKJ43_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71434.1"
                     /translation="MDKKKFKTGFVVGIILGALVAAAALYYYDQQYRKSDLEKAMDKI
                     EKKATKDFDKAAKEVDKLFEE"
     gene            1169..1507
                     /gene="rbfA"
                     /locus_tag="PKJ43_02370"
     CDS             1169..1507
                     /gene="rbfA"
                     /locus_tag="PKJ43_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNZ71435.1"
                     /translation="MDSIRQNKVSRLIQKELAEIFQHESRELFRGKMISVTVVRVSKD
                     LSVAKIYLSIFPSEGNEEIMKLIKQVSPQIRGLLGNKVGKQLRIIPELAFFVDDSLDY
                     IDNIDNILKH"
     gene            1513..2325
                     /locus_tag="PKJ43_02375"
     CDS             1513..2325
                     /locus_tag="PKJ43_02375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ71436.1"
                     /translation="MQYDPIKKSLGNVFNKSPFLRKLFYRLLDLLLLRSWHIRRELRV
                     WKAPASPVILDAGSGFGQYSYRMARLFPNALIKAVDVKQEQITDCNQFFLKIGLNNRV
                     IFEKADLTKYIEENQFDLILSVDVMEHIEEDVLVFKNFYSSLTKNGMLLISTPSDMGG
                     SDSHDHDQNEVSGFIDEHVRDGYNIEDIKDKLRSVGFSKVSARYQYGSAGKISWVLSM
                     KFPIMMLGITKLFFIILPFYYLIAYPISALLNWIDVTANHKSGTGLIVTATK"
     gene            2383..3546
                     /locus_tag="PKJ43_02380"
     CDS             2383..3546
                     /locus_tag="PKJ43_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNZ71437.1"
                     /translation="MVNIISAIALVGVVVGTMALVVVLSIFNGFDVLIKSFFSVFDPE
                     IKITSVEGKTFDPTLDALQKLKNHENVIHFCEVVEEIAHFRFEERQHIAYIKGVEDNY
                     FKMTQLDSFMYDGKLQLVEDNIPYTVLGHGLAYNLGASPNFIHPVFISVPKKGKSTSI
                     LANPFNQKYVYTSGIYSVGQQEVDDMFAIIPLEMARELTNAQGKVTSIEIGLKPGTNL
                     KRFQRDIQKMVGNGFKVQNRYQQHESYYKVTESERFFIYIILSFILVIASFNLVSSIA
                     MLILDKRKDINILVSMGLTRKQLSQVFLFEGWLVSLVGSIIGLFLGTLFCLGQMYFGW
                     IKFPGNFAVEYYPVDLRVSSLIIIAITVLVIGGAASWLPVKFLPEKFFQMREE"
     gene            complement(3573..>4061)
                     /gene="pepE"
                     /locus_tag="PKJ43_02385"
     CDS             complement(3573..>4061)
                     /gene="pepE"
                     /locus_tag="PKJ43_02385"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF003642.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HNZ71438.1"
                     /translation="MREAIEEAEAIVVGGGNTWQLVKLMHQNSLFDVIRRKVQLGTPY
                     VGWSAGSNVACPTLRTTNDMPIVDPMSFETLNLIPFQINPHYLDANPDGHAGETREQR
                     IEEFIEVNPEVYVAGLREGCMLKVEGKSMTLIGERSLRVFKKGNLPVELSSSDDLSFL
                     LR"
BASE COUNT         1197 a          867 c          780 g         1217 t
ORIGIN      
        1 aaactaaaat atttctgatt gtcgaaaaac atttgagtta caatgtaatc ggccccagca
       61 tcgacctttt gtttcagata acgcagatcg gtatcgaggt ttggtgcttc gaagtgtttt
      121 tcgggatacc cggcaacccc aatgcaaaaa tcgagcgatg ctttattttt caaatctggg
      181 tcgaggtatt ggccttttcc aagagaagca atttgctcga ccaattcatt ggcgtttttg
      241 ttgccatctt tctcaggggt aaacaccgac tcttgtttca atccatcacc acgaagcgcc
      301 agcacatttt caattcccag aaaatcgaga tcgatcagca catgctcagt ttcactcctt
      361 gaaaatcccc cgcagagaat atgtggcaca acaggaattc catatttatg ctgaatagct
      421 gcagcaattg caaccgttcc gggcctttta cgcaccactc ttttttcaat aagaccatct
      481 tcacgtgtcc tgaattcgta ttcgtcgcga tgggtggtaa tattgatata ttttgggtca
      541 aactcaagca gttgatcaat tgtgctataa agtttatcgc ctttgctccc tttcaacggg
      601 ggtaacaact caaacgagaa aagtgtcttt tccgattttt taagaatatc tataactttc
      661 attcttcaaa caatttatcg acttctttag ctgctttatc gaaatctttt gtagcttttt
      721 tttcgatctt gtccattgct ttttcaagat cggattttcg gtattgttga tcatagtaat
      781 acaacgctgc agcagcaacc aacgcgccaa gaattattcc aacaacgaat cctgttttaa
      841 attttttctt gtccattgtt attttgttga attcgacgcg aaaatacaaa taattcgaca
      901 ccaattaaaa cgcctctgca acaccagtca ctcataatgc aaaacaaaac cctcaagggc
      961 cagaatttca gcgcaaatct aattcaaaaa tcgttgtgca acagaaagaa acgcaggcaa
     1021 gtgcttataa atgaaaaata actaatgcaa caaaaatata aacagtaatt ttacccacac
     1081 gagaaaagca catactttga agccaaaacc aataaatgtt acatttgaat cgttaacttt
     1141 gttgacttac aattatttct aagtagctat ggattcaata cgtcagaata aagtttcaag
     1201 gctcatacaa aaggagttag ccgaaatttt ccagcatgaa agccgagaac ttttccgtgg
     1261 aaaaatgatt tcggttaccg ttgtgcgggt atccaaagat ttatcagttg ccaaaatata
     1321 tctcagcata tttccttctg aaggaaatga agaaataatg aaactgatta aacaggtttc
     1381 cccacaaatt cggggcttgc tgggcaataa agtgggcaaa caactacgaa ttatacctga
     1441 attggccttt tttgtcgatg atagcctgga ttatatcgac aatattgata acatactcaa
     1501 acattgattt ttatgcaata cgatccaatt aaaaagagct taggaaacgt tttcaacaaa
     1561 tcgccttttc tccggaagct tttctaccgt ttgctcgatc tgttattgtt acgttcgtgg
     1621 cacatacgcc gtgaacttcg ggtctggaaa gcacctgcaa gtccggtaat tctcgatgcc
     1681 ggttcaggtt ttggccaata tagctatcgc atggcaaggt tattcccgaa tgcactgatt
     1741 aaagctgtcg acgtaaaaca ggaacaaatt accgattgca accaattttt tctgaaaatc
     1801 gggctgaata accgggttat ttttgaaaaa gcagatctaa caaaatatat tgaagaaaat
     1861 caatttgacc tcatcctatc ggttgatgtt atggaacaca ttgaagagga tgtgttggtt
     1921 ttcaagaact tctattcgtc gctcaccaaa aatggcatgc tgctgatttc gaccccaagt
     1981 gatatgggcg gttccgactc gcacgaccat gaccagaatg aagtttcagg ctttattgat
     2041 gaacatgtgc gcgacgggta taatatcgaa gacattaaag acaaattgcg gtcggttggt
     2101 ttcagtaaag tttcggcacg ttaccagtat ggtagtgctg gaaaaatatc gtgggtgctt
     2161 tccatgaaat ttcctattat gatgctggga ataaccaaat tattctttat cattttgcca
     2221 ttttactacc ttattgccta tcctatttca gccttgctca actggatcga cgttacagca
     2281 aaccacaaat cgggtacagg cttaattgta acagctacca aataattgaa tatatcgtac
     2341 tacatagcac ggaggtacct catttcaaaa aaatcaaaaa atgtggtgaa cattatttcg
     2401 gctattgcgc ttgtgggcgt agtggttgga acaatggcgc ttgtggttgt tctttcaatt
     2461 ttcaatgggt tcgatgtcct gatcaaatca tttttcagtg ttttcgaccc cgaaataaaa
     2521 attacatcgg ttgaaggaaa aacattcgac ccaacccttg atgctcttca aaaactaaaa
     2581 aaccacgaaa atgtaatcca cttttgtgaa gttgttgaag aaattgccca tttcaggttc
     2641 gaggaacggc aacatattgc ctacatcaaa ggggttgaag acaactattt caaaatgact
     2701 cagctcgaca gttttatgta cgatggaaaa ttacaactcg tagaagacaa tataccttac
     2761 actgtgcttg ggcatgggct ggcctataac cttggtgctt caccaaattt tattcatcct
     2821 gtttttattt ctgtacccaa aaaaggcaaa agcaccagta ttctggcaaa tcctttcaac
     2881 caaaaatacg tatatacttc aggtatttat tctgttgggc agcaagaagt tgacgatatg
     2941 tttgccatta ttccccttga aatggcccgc gaactgacca atgcacaagg gaaagtaacc
     3001 tcgatagaaa tcggactcaa gcctggaaca aacctgaaac gttttcaacg cgacattcag
     3061 aaaatggttg gaaatggatt caaggttcag aatagatacc aacaacacga atcgtattac
     3121 aaagtaactg agagtgagcg ctttttcatc tatatcattt tatcgttcat tttggtcatc
     3181 gcttccttca accttgtcag ctcaatagct atgcttatcc tcgacaaacg aaaggatatc
     3241 aatattttag taagtatggg gctgacgcgg aaacaacttt cgcaagtatt tctttttgaa
     3301 ggatggctcg tttcgctggt tggctcaatt atcggattat tccttggaac cctcttttgc
     3361 cttggccaga tgtactttgg ctggataaaa ttccccggca attttgctgt agagtattat
     3421 cccgtcgacc tcagggtttc aagtttgatt atcatcgcaa taaccgttct tgttattggc
     3481 ggtgctgcct cgtggcttcc tgtgaagttc ctgcccgaga aatttttcca aatgagggaa
     3541 gaatagtttc tccgcccttt aactattcaa atttatcgta aaaggaacga aagatcgtca
     3601 gacgaagaaa gttcgaccgg cagatttccc tttttaaata cccgcaaaga gcgctcacca
     3661 atgagcgtca tcgatttccc ttccacctta agcatacagc cttcgcgtaa cccggccaca
     3721 tacacttctg ggtttacctc gataaactct tcgattcgct gctcccgtgt ttcgccagca
     3781 tgcccgtcgg ggttggcatc aaggtagtgc gggtttatct gaaaaggtat caaattgagg
     3841 gtttcaaaac tcatcggatc aacaataggc atatcgtttg tggttcgcag cgtggggcaa
     3901 gcaacgttcg atccggcact ccaacctaca tagggagttc cgagttgtac ttttcgccga
     3961 ataacatcaa acaaactatt ttgatgcatg agctttacca attgccatgt gttccctcca
     4021 cctacaacaa tcgcttctgc ttcttcaatc gcctcgcgca t
//
LOCUS       DAOPUW010000088         4060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_22274_length_4060_cov_3.981773, whole genome shotgun sequence.
ACCESSION   DAOPUW010000088 DAOPUW010000000
VERSION     DAOPUW010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22274_length_4060_cov_3.981773"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PKJ43_02390"
     CDS             <1..357
                     /locus_tag="PKJ43_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ71439.1"
                     /translation="NAGNRLLLQYNLEGHFDIDFAGNGLSAIDKFTQNKYDLILMDIH
                     MPEMDGVETAIRIRQIENGKWRTPIFAITSNAYNEQRARCFDAGMDDYIVKPIHAKAL
                     VEHINLFLNSASFGAM"
     gene            complement(815..1165)
                     /gene="rplQ"
                     /locus_tag="PKJ43_02395"
                     /pseudo
     CDS             complement(815..1165)
                     /gene="rplQ"
                     /locus_tag="PKJ43_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017042856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(1172..2164)
                     /locus_tag="PKJ43_02400"
     CDS             complement(1172..2164)
                     /locus_tag="PKJ43_02400"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNZ71440.1"
                     /translation="MAILAFQKPDKVIMLESDERFGKFEFRPLEPGYGITIGNALRRI
                     LLSSLEGYAITTIKIEGVEHEFATIKGVIQDVTEIILNLKQIRLRKEIEDFDSEKVSV
                     TITGQDKFTAGNLNKFMTGFSVLNPDLVICEMEPAVKLQMEFTINKGRGYIPASENKP
                     NEEEFGVIPIDAIYTPIKNVKFSVENFRVEQKTDYEKLLIEILTDGSIHPKDALKEAA
                     KILIYHFMLFSDEKITLDSDEKFANEEFDEEILHMRQMLKTKLVDMDLSVRASNCLKA
                     ADVETLGELVQYNRNDLLKFRNFGKKSLTELDDLLSAMNLNFGMDISKYKLDKE"
     gene            complement(2184..2792)
                     /gene="rpsD"
                     /locus_tag="PKJ43_02405"
     CDS             complement(2184..2792)
                     /gene="rpsD"
                     /locus_tag="PKJ43_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNZ71441.1"
                     /translation="MARYIGPKSKVARKFGEPIYGPDKVLERKNYPPGFHGMARRRRK
                     ISEYGVQLKEKQKVKYTYGVLERQFRKLFEKALRSKGITGEVLLILLESRLDNVVYRL
                     GIAPSRAAARQLVSHKHITVNGLVCNIPSCLLQPGDVVGVREKSKSLEIITESLNSRR
                     YSKSAWLEWDNALMTGKYLNNPEREEIPENIKEQLIVELYSK"
     gene            complement(2815..3207)
                     /gene="rpsK"
                     /locus_tag="PKJ43_02410"
     CDS             complement(2815..3207)
                     /gene="rpsK"
                     /locus_tag="PKJ43_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNZ71442.1"
                     /translation="MAKKSGTTSKKRVVKVEATGQAHVHSSFNNIIVSMANDNGEVIS
                     WSSAGKKGFRGSKKNTPYAAQTAAEECAKTAYDLGLRKVKVFVKGPGNGRESAVRAIA
                     SVGIQVTEIVDVTPLPHNGCRPAKRRRV"
     gene            complement(3222..3599)
                     /gene="rpsM"
                     /locus_tag="PKJ43_02415"
     CDS             complement(3222..3599)
                     /gene="rpsM"
                     /locus_tag="PKJ43_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNZ71443.1"
                     /translation="MARIVGVDIPNNKRGEIALTYIYGIGRSSAGRILAEAGVDKNMK
                     VQEWTDDMFQAIRTIIGSTMKVEGELRSETQLNIKRLMDIGCYRGIRHRIGLPVRGQS
                     TKNNARTRKGKRKTVANKKKATK"
     gene            complement(3629..3745)
                     /gene="ykgO"
                     /locus_tag="PKJ43_02420"
     CDS             complement(3629..3745)
                     /gene="ykgO"
                     /locus_tag="PKJ43_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HNZ71444.1"
                     /translation="MKVRVSIKKRSADCKIVRRKGRLYVINKKNPKFKQRQG"
     gene            complement(3856..4056)
                     /gene="infA"
                     /locus_tag="PKJ43_02425"
     CDS             complement(3856..4056)
                     /gene="infA"
                     /locus_tag="PKJ43_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNZ71445.1"
                     /translation="MEQDGTIIEALSNAMFRVELENGHVITSHISGKMRMHYIKILPG
                     DRVRVEMSPYDLTKGRITFRYK"
BASE COUNT         1149 a          844 c          717 g         1350 t
ORIGIN      
        1 aatgcgggaa acaggttatt acttcaatat aatctggagg ggcattttga tatcgatttt
       61 gccggcaatg gactttctgc tatcgacaaa tttactcaga ataaatacga tttgatttta
      121 atggatatcc acatgcccga gatggatgga gttgaaacag ccatcagaat caggcaaatt
      181 gagaacggta agtggcgaac tccaatcttt gctataactt caaacgctta caatgaacaa
      241 cgagctcggt gctttgatgc cggaatggac gattatattg tgaaacccat tcatgctaaa
      301 gcacttgtcg aacacatcaa tctctttttg aattctgcat cattcggagc catgtaaaat
      361 attaaagatc tgttttgaaa cagcattaat cgttgtcagg aaatagttcg caatcagcat
      421 cagttagttg aaaatcatac gaaatatcca aagaaggaat ttggaaacta ggatagtttg
      481 tctgagaatt tgcatgcatc tagtaaaaaa gtaccctctg agaatactaa cgtatcaaaa
      541 tgtcagaaga atgacctccc aactatcata aacacataga atataaaata aaaaaggttg
      601 acattttatg ccaacctttt tttgaatatt gtatcgaaca ttattcttta ccttcttcgg
      661 tactttcaga agcgacttcc tttgcaggtt catctacaac tgttgcagcc tgacttttag
      721 cagctactgc accctcagtt ttctttttgg caccacgcct gcgacgtgac gaagcttttt
      781 tgtcgtcttt tgcagcaagc atattttcat tgtaatcgac caattcgagg atacacattt
      841 ctgcatcatc acctaacctg tttccgattt taaggatacg ggtatatcct cctggacggt
      901 cagcaatttt aggtgaaaca tcacggaaga gttcagaaac tgattctttg tctttcaggt
      961 aactgaaaac tacacgacgg ttttgtgttg tatcttcttt cgatttagtg atcaaaggtt
     1021 caacatacat tttgagagcc tttgcttttg ctgtggtagt tgttattctc ttatgcagaa
     1081 tcagcgagca agccatattc gaaagcatgg ctttacgatg agcgcttttt ctgcctaagt
     1141 gattaatttt cttattatgt ctcatcgtta actattcctt gtctagttta tacttactaa
     1201 tatccattcc aaaattaagg ttcatagccg aaagtaggtc atccaactcg gtaagcgact
     1261 ttttaccaaa attgcggaat ttaagcagat cgttacggtt atactgaact aattcaccaa
     1321 gagtttcaac atctgcagct ttgaggcagt tcgatgcacg aacagaaaga tccatgtcga
     1381 caagcttagt tttaagcatt tgacgcatat gcaaaatttc ttcatcgaac tcttcattag
     1441 caaatttctc atccgaatcg agagtaatct tttcgtcgga gaaaagcatg aagtgataaa
     1501 taagaatttt tgcggcttct ttcaatgcat cttttggatg aatcgaccca tcggtaagaa
     1561 tttcaattaa gagtttttcg tagtcggttt tttgttccac acgaaaattc tcaacagaaa
     1621 acttcacatt tttgatcgga gtataaatcg catcgattgg aataaccccg aattcctctt
     1681 cattgggttt attctcagaa gcaggaatat aaccacggcc tttgttgatg gtgaattcca
     1741 tctgaagctt tacagctggt tccatttcgc aaataacaag gtcaggattt aatacactga
     1801 accctgtcat gaacttattc aggtttcctg ctgtgaattt gtcttgcccg gtgatagtta
     1861 cagagacttt ttcgctgtcg aaatcttcga tttcttttct caacctaatt tgtttcaagt
     1921 taagaatgat ttcggttacg tcttgaatta cacctttaat agtggcaaat tcatgctcaa
     1981 ctccttcaat tttgatggta gttattgcat atccttccaa agatgataat aaaatcctac
     2041 gaagggcatt accgatagta ataccataac caggctctaa cgggcgaaat tcaaattttc
     2101 cgaatctttc gtccgattca agcattatca ctttatcggg cttttgaaaa gctaatattg
     2161 ccatagtttt ttctctaatg tgattatttc gaatacaact ctacgatcag ttgttcttta
     2221 atgttttcgg gtatttcttc acgttcaggg ttattcaaat atttccctgt catgagtgca
     2281 ttatcccatt ctaaccaagc acttttgcta taacgacgtg agttgagtga ctcggtaata
     2341 atttcgagag actttgattt ttcacgaacg ccaacaacat ctcccggttg taataaacaa
     2401 gaaggaatat tacatacaag gccgttaacg gtgatatgct tatgcgaaac taactgacgg
     2461 gcagcagcac gcgatggagc aatcccaaga cgatacacaa cgttatcgag cctcgattcg
     2521 aggagtataa gtaaaacttc accggtaata ccttttgaac gcagagcttt ttcaaaaagt
     2581 ttacggaatt gacgttcaag aacgccataa gtgtatttta ctttttgttt ttcttttaac
     2641 tgaacaccat attcagatat ttttctgcga cggcgtgcca ttccatggaa cccaggaggg
     2701 taattcttgc gttccaatac cttgtcgggg ccataaatag gttcaccgaa tttcctggct
     2761 acttttgact ttggtccaat atatcttgcc atttcttact ttttattgca ataattaaac
     2821 acgtcttctt ttagcaggcc gacatccatt gtgtggtaac ggagttacat caacaatctc
     2881 agttacctga attccaacgc tagcaatagc cctaacagcc gattcacgtc cgttacctgg
     2941 gccctttaca aatactttta ctttacgtaa tccaagatcg tatgcagttt ttgcacactc
     3001 ttcagcagcg gtttgagcgg cataaggggt attcttttta gaacccctga aacccttttt
     3061 acctgccgaa gaccaggata tcacttcacc attatcgttt gccatcgata caatgatgtt
     3121 attaaacgat gagtgaacgt gagcttgtcc tgtggcttca accttaacga ctcttttctt
     3181 tgaagtagtt cctgactttt ttgccataat ctatcaccta attatttagt cgctttcttc
     3241 ttgtttgcaa ctgttttacg tttacctttt cgggtacggg cattattttt agtactctgt
     3301 ccacgtactg gcaagccaat acgatggcga ataccgcgat agcaaccgat atccattaag
     3361 cgtttaatat ttagctgtgt ttccgaacgg agttctcctt ccactttcat ggttgaaccg
     3421 ataatggtac gaatcgcttg gaacatatca tctgtccatt cctgaacttt catgttctta
     3481 tcgacaccag cttcggcaag aattcttcct gcactgctgc gaccaatccc gtagatatac
     3541 gttagcgcta tttcgccacg tttgttgttc ggaatgtcaa caccgactat acgagccata
     3601 aatttctaat tatttgttgt tttacaaatt aaccctgacg ttgtttgaac tttggattct
     3661 ttttattaat cacatacaaa cgacctttcc tgcgaacgat cttacagtca gcgctacgtt
     3721 tcttaattga tacacgtact ttcattcatt tctaatttta agaatggttt cccatccaac
     3781 gtcacttttt ttgaaaattg gcacaaaaat acatttttta ttgtgacaat tccaacagtc
     3841 ccgtaaatct attgattatt tgtatctaaa tgttatccta cctttagtaa ggtcgtaggg
     3901 tgacatttca accctaaccc tatctccagg taagatttta atgtaatgca tcctcatctt
     3961 accagagatg tgagatgtaa ttacatgtcc attttcaagt tcaacacgaa acattgcatt
     4021 agacaatgct tcgataattg taccatcttg ctcaatagaa
//
LOCUS       DAOPUW010000089         4060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_22276_length_4060_cov_2.907116, whole genome shotgun sequence.
ACCESSION   DAOPUW010000089 DAOPUW010000000
VERSION     DAOPUW010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22276_length_4060_cov_2.907116"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..446
                     /gene="rplK"
                     /locus_tag="PKJ43_02430"
     CDS             6..446
                     /gene="rplK"
                     /locus_tag="PKJ43_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNZ71446.1"
                     /translation="MAKEVAGIIKLQIKGGAANPSPPVGPALGSKGVNIMQFCKAFNA
                     RTQELAGKVLPVVITVYADKSFDFIVKQPPVAIQLLEAAKVKSGSAEPHVKKVGSISW
                     NQVKEIAEVKMSDLNCFTLESAMRMVAGTARSMGITITGDSPFN"
     gene            466..1161
                     /gene="rplA"
                     /locus_tag="PKJ43_02435"
     CDS             466..1161
                     /gene="rplA"
                     /locus_tag="PKJ43_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNZ71447.1"
                     /translation="MARITKNRKLALEKIGQDKIFTLEAASKLVKEITSTKFDASVDI
                     DVRLGVDPRKANQMVRGVVSLPHGTGKEVRVLALVTPDKVQEATEAGADYVGLDDYLE
                     KIKGGWADVDVIITMPPIMGKLGPLGRFLGPRGLMPNPKSGTVTMEVGKAVKEIKQGK
                     IDFKVDKYGIVHTSVGKVSFEPEKIVDNAKEFVNTIVKLKPVAAKGTYIKSIYLSSTM
                     SPGIQVDPKSAQD"
     gene            1172..1702
                     /gene="rplJ"
                     /locus_tag="PKJ43_02440"
     CDS             1172..1702
                     /gene="rplJ"
                     /locus_tag="PKJ43_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNZ71448.1"
                     /translation="MKRSEKQTIIDNLVQEINSYNHFYLADISDLNAEKTSELRRVCF
                     QKDVKLIVVKNTLLRKALEASGKNADELFVTLKGNTSLMFSENGSLPAKLIQDFRKKN
                     KSLSKPQFKSAFVEECVYVGENQLDVLATIKSKEELIGDIVALLQSPAKNVISALQGG
                     GGQKIVGILKTLSEKE"
     gene            1737..2076
                     /gene="rplL"
                     /locus_tag="PKJ43_02445"
                     /pseudo
     CDS             1737..2076
                     /gene="rplL"
                     /locus_tag="PKJ43_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
     gene            2232..>4060
                     /gene="rpoB"
                     /locus_tag="PKJ43_02450"
     CDS             2232..>4060
                     /gene="rpoB"
                     /locus_tag="PKJ43_02450"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNZ71449.1"
                     /translation="MFEYPDFLEIQIKSFIEFFQLGSTPEIRKQEGLYKVFMENFPIT
                     DTRNNFVLEFIDYYIDPPRYSIEECIERGLTFSVPLKAKMKLYCTDPEHEDFDTVVQD
                     VYLGTIPYMTERGTFVINGAERVIVSQLHRSPGVFFGQSMHANGTKLYSARIIPFKGS
                     WIEFATDINNVMYAYIDRKKKLPVTTLLRAIGFESDKEILEIFGLADEIKVSKVALKK
                     LVGRKLAARVLKTWVEDFVDEDTGEVVSIERNEVIIDRETVIENDHVDMIVDSGTKTI
                     LLHKEEANLQDYAIIYNTLQKDPCNSEKEAVLHIYRQLRNAEPPDESTAREVIENLFF
                     SDKRYDLGNVGRYRINKKLNLEIDIDTRILTNDDIIAIIKYLIQLVNSRTDVDDIDHL
                     SNRRVRTVGEQMFGQFGVGLARMARTIRERMNVRDNEVFTPIDLINSKTLSSVINSFF
                     GTNALSQFMDQTNPLAEMTHKRRMSALGPGGLSRERAGFEVRDVHYTHYGRLCPIETP
                     EGPNIGLISSLCVFAKITDLGFISTPYRKVNNGVVDLTDEGVIYLTAEEEEGQVIAQA
                     NAKIDDTGRFINPKIKARLEGDYPLVEPENVQLMDVGPNQIAS"
BASE COUNT         1302 a          737 c          866 g         1155 t
ORIGIN      
        1 ttgttatggc taaagaagtt gctggaatta tcaaactcca aattaaaggt ggcgcggcta
       61 atccgtcacc accggttgga ccagcattgg gatccaaagg ggtaaacatt atgcagtttt
      121 gcaaggcatt taatgcccgt acgcaggaac ttgccggtaa ggtgcttcct gttgttatta
      181 ctgtgtatgc cgataaatca ttcgatttta tcgtaaaaca accccctgtg gctattcaac
      241 ttctcgaagc agctaaagtt aaaagtggct cagctgaacc tcatgttaag aaagtaggat
      301 caatttcctg gaatcaggta aaagaaattg ctgaagtaaa aatgagtgac ttaaactgct
      361 ttacgttaga atctgctatg agaatggttg ctggtactgc ccgtagtatg gggattacta
      421 tcacaggtga ttctccattt aattaaacat ttaaaattga ttaaaatggc tagaattact
      481 aagaatagaa aacttgcttt agaaaaaatc ggtcaggaca aaatctttac gctcgaagct
      541 gcatcaaaat tggttaagga aattacctct actaagtttg acgcttctgt cgatatcgat
      601 gtgcgtttag gtgttgaccc ccgaaaagct aaccaaatgg ttcgtggcgt agtttcgctg
      661 cctcacggaa ctgggaaaga agtaagagtt ttagctttgg ttactccaga taaggtgcaa
      721 gaagctaccg aagctggagc agactacgta ggtttggatg actaccttga aaaaatcaaa
      781 ggtggttggg ctgatgttga tgttattatc actatgcccc caattatggg aaaattaggc
      841 ccgttaggcc gtttccttgg tcctcgtggt ttgatgccga atcctaaaag cggaactgtt
      901 acaatggaag ttggtaaagc tgtaaaagaa atcaagcaag gtaagatcga ctttaaagtc
      961 gataaatatg gtattgtgca cacatctgta gggaaagtat cattcgaacc cgaaaagatt
     1021 gttgacaatg ctaaggaatt tgttaataca atcgtgaaac tgaaaccagt ggcagcgaag
     1081 ggaacataca ttaaaagtat ttacctgtcg agcacaatga gccccggcat tcaggttgac
     1141 ccaaaatcag cacaggatta atttgtgaat tatgaagaga tcagaaaaac aaacgattat
     1201 cgacaatctc gtacaagaga tcaattcata caatcatttt tacctggcag atatttctga
     1261 cctgaacgca gaaaaaacga gcgaattgcg ccgtgtctgc ttccagaaag atgtaaaact
     1321 gattgtagtt aaaaacactc tacttagaaa agctttagag gcatcaggaa aaaatgccga
     1381 tgagcttttt gtgactctta agggcaatac atcattgatg tttagcgaaa acgggagcct
     1441 tcctgcaaaa cttattcaag atttccgcaa gaagaacaag agcctttcaa aaccacaatt
     1501 caaatcagct tttgttgaag aatgtgttta tgtaggtgaa aatcaactcg acgttttggc
     1561 aaccatcaaa tccaaagaag aactcattgg agatatcgtt gctcttcttc aatctccggc
     1621 caaaaatgtc atttctgcct tacaaggtgg cggtggacag aaaatcgtcg ggattcttaa
     1681 aactttatca gagaaagaat aataaattat tgataacatt ttaaatcgtt gttaaaatgg
     1741 cagatttaaa acaacttgca gaagaattag ttaaccttac tgttaaagaa gtaaacgaac
     1801 tagcaggtat attgaaatca gaatatggta ttggtagctg ctgaagctgc tgaagaagct
     1861 gctcaaactg aattcgatgt aattatgaca tcagcaggac aatcaaaact tgctgtagtt
     1921 aagttagtta aagaattaac tggtcttgga ctgaaagagg ctaaagaact cgttgatgct
     1981 gctccaaaag ccatcaagga aaaagtttct aaggaagagg ctgaagctct taaagcacaa
     2041 ttaattgaag caggagctga ggttgaactt aaataaggtc tacaacccct caaaagggtt
     2101 acaatggttt agtcccggcg tacgtcggga ttaagccttt ttgtgtttta tgttttaagt
     2161 tcaataaata cattatttag atgtctttgg atactgttag tcagagaatt agtttttcgg
     2221 tatcgaaaaa catgttcgaa taccccgatt ttcttgagat tcaaatcaag tctttcatag
     2281 agttttttca attaggttct actcccgaaa ttcggaagca agaggggctc tataaagttt
     2341 tcatggaaaa cttccccatc accgacaccc gtaataattt cgttctcgaa tttatcgatt
     2401 attatatcga tccaccacga tattctatcg aagagtgcat tgaaagagga ttaactttca
     2461 gtgtaccttt gaaagccaag atgaaacttt actgtaccga tcccgaacac gaagatttcg
     2521 ataccgttgt acaggatgtt tatcttggaa caatccccta catgaccgaa agaggtactt
     2581 ttgttatcaa cggtgccgaa cgtgttatcg tttcgcagtt gcatcgctcg cctggtgtat
     2641 tcttcgggca gagtatgcat gccaacggaa caaaattgta ttcggctaga atcattccgt
     2701 tcaaagggtc atggattgag tttgcaactg acattaacaa tgttatgtat gcatacatcg
     2761 atcggaaaaa gaaattgccg gtaacaacat tattacgagc catcggtttc gaaagcgata
     2821 aagaaatact cgaaattttt ggacttgccg atgagattaa agtctcaaaa gttgccttga
     2881 aaaaacttgt tggcaggaaa cttgctgccc gtgttttgaa aacatgggta gaagactttg
     2941 ttgacgaaga taccggtgaa gtagtatcca ttgaacggaa tgaagtcatt atcgatcgcg
     3001 aaactgttat tgaaaacgat cacgttgata tgattgtcga ttctggcaca aaaacaattc
     3061 ttttgcacaa agaagaagct aatcttcagg attatgcgat aatatataat acccttcaga
     3121 aagatccatg taactctgaa aaagaagcag ttcttcatat ttacagacag ttgcgtaacg
     3181 cagaaccgcc tgatgaatct actgcacgtg aagtaattga gaacttattt ttctcagata
     3241 aacgttacga tctagggaat gttggacgtt acaggatcaa caaaaaattg aatcttgaaa
     3301 ttgatatcga tactcgtatc cttacgaatg atgatatcat cgcaattata aaatacctta
     3361 tccagttagt caattcccgt accgatgttg acgatatcga ccacttgtcg aatcgtaggg
     3421 tgcgtacagt tggcgaacaa atgtttggac aatttggtgt tggtttggcc cgtatggccc
     3481 gtacaattcg cgaacgtatg aatgttcgtg acaatgaagt gtttacgccg attgatctta
     3541 ttaattcaaa gacactttct tcggtaatta attctttctt tggtaccaat gcgttatcgc
     3601 agttcatgga tcaaaccaat ccgctggccg aaatgactca caaacgccgt atgtcggcat
     3661 tgggacctgg aggtttgtcg cgtgaaagag caggtttcga agttcgtgac gtgcactata
     3721 ctcactatgg ccgtttatgt ccgatagaaa ctcctgaagg accaaacatt gggcttattt
     3781 cttcattatg cgtttttgcc aagattactg acctcggatt tatttcaaca ccctaccgta
     3841 aagtaaataa cggagtagtt gatttaactg acgagggcgt aatatatttg actgccgaag
     3901 aagaagaagg acaggtaatt gctcaggcaa atgcgaagat tgatgataca ggacgcttta
     3961 tcaatccaaa aatcaaagca agactagaag gcgattatcc attggttgaa ccagagaatg
     4021 ttcaactcat ggacgttgga ccgaatcaga ttgcatcaat
//
LOCUS       DAOPUW010000090         4057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_22297_length_4057_cov_3.368566, whole genome shotgun sequence.
ACCESSION   DAOPUW010000090 DAOPUW010000000
VERSION     DAOPUW010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22297_length_4057_cov_3.368566"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="PKJ43_02455"
     CDS             complement(<1..1093)
                     /locus_tag="PKJ43_02455"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNZ71450.1"
                     /translation="MRKVIRNIILVFNICVALALLASYLVSEVNPVKFWIPSVLGLAF
                     PYLLLINVLFVFFWMFFKRQFILISAVCIVIGMHINTSYFQFLPRHTARKEGIKVLSY
                     NVQNFYSYLEKKKDNDAIIDFIAAQKANIICLQETKLQKEGDLNPIKLKSMFPGIKHC
                     QLAHQSKWGGPVTFTSYPIVSMGEIRFEDTKNMVIFTNIKIGNDTVRVYNCHLQSFGI
                     NTSAYSVIDDPGLDSQKMKEMRTIASKLKWGNIYRAKQVVKLSGHIAACPYPVIVCGD
                     FNDTPISFTYGEISAKLKDSFVESGSGVSNTYRGKLPQNRIDYIFHSKHFKAYNYTRH
                     KVEFSDHYPISSFLVKRKSRKCRRQQLFFD"
     gene            1308..2273
                     /locus_tag="PKJ43_02460"
     CDS             1308..2273
                     /locus_tag="PKJ43_02460"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNZ71451.1"
                     /translation="MRSNKTGNSQLKRKTTTPAQIAKKKSDSRIKSKPANKATSKQAG
                     KGIERNSRVEVIPTGKTEKPVVERKKPTLSTPAKRGFRPDEKIRLNRFLANSGVCSRR
                     EADVYINTGCVTVNGKITTELGTKVSLSDDVRFNGQSLCPEKKVYLLLNKPKGYVTTT
                     DDPQERKTVMELVVNACPERIYPVGRLDRDTTGLLLFTNDGDLAKKLTHPSHSRKKIY
                     QATLNKQLTKNDLLSVARGIDLDDGPIAADSINYVDEEDKREIGIEIHSGRNRIVRRI
                     FEHLGYEVTKLDRVYFAGLTKKNLPRGKWRFLTQAEINQLKMLKV"
     gene            2464..2943
                     /locus_tag="PKJ43_02465"
     CDS             2464..2943
                     /locus_tag="PKJ43_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNZ71452.1"
                     /translation="MFLNIVTQNQGIIHKVCGIYCDTHEDRQDLFQEIVSQLWRSFPT
                     FRNESKVSTWMYQVSLNTAITHFKKNKKRPDRSEIGKSIYLIPDEIYDDTFENQVKVL
                     HKAIAKLTGIEKSIVLLYLEDKKYEEIAEIIGITQNYVRVKMNRIKGKLKKLMGEEA"
     gene            2943..3647
                     /locus_tag="PKJ43_02470"
     CDS             2943..3647
                     /locus_tag="PKJ43_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71453.1"
                     /translation="MELEDLKKTWDKISSENEKRQLNQEDIDTLLKNRTLDITEKIRR
                     NIRIGLIIILAWIGLWFAIEMLFTPLLEKFISEPSLSGDLVNWMTAIEGFTYLLIIAA
                     IIVFGVNYNRIEKQNSTQINLKKKITLHIRTLNLYKTMFYIILIIILLNITVAFSTGF
                     ISGFNSQTVAQQLNISNISFFKWLIIGMSFLLTLGIVISIYYFLFHLFFNRLYGKYLK
                     QLKNTLKEFDEVNSLQ"
     regulatory      3712..3898
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         1378 a          832 c          728 g         1119 t
ORIGIN      
        1 aatcaaagaa tagctgctgc cttctgcact ttctactttt tctttttact agaaatgaac
       61 ttatcggata atggtcagaa aattccacct tgtgtcgggt atagttatat gccttaaagt
      121 gcttcgaatg aaatatataa tcgatacgat tctgaggaag cttgccccgg tatgtatttg
      181 aaacaccact ccccgactca acaaaagaat ccttcaattt ggcgctaatt tcgccgtatg
      241 tgaacgaaat aggagtgtca ttaaagtcgc cgcaaacaat tacaggataa ggacatgcag
      301 caatatgccc gcttaatttc acaacttgct tcgcccgata aatattcccc cacttaagtt
      361 ttgaagcaat tgtacgcatt tccttcatct tttgagaatc aaggccggga tcgtcaataa
      421 cagaatatgc agatgtatta attccaaacg actgcaaatg acaattatac accctcacgg
      481 tatcgttacc aatcttaata ttagtgaaaa tcaccatgtt ctttgtgtct tcaaaacgaa
      541 tttcccccat cgaaactatg gggtaactgg taaaagtaac tggccctccc cacttgcttt
      601 ggtgcgccaa ctgacagtgc ttaatacccg ggaacatgct ttttaactta ataggattca
      661 gatctccttc tttttgaagt tttgtttcct gcaagcatat aatgttagcc ttttgcgctg
      721 caataaaatc aataatagcg tcattatcct tctttttttc gaggtaagaa taaaaatttt
      781 gcacgttata actcaatact ttaattcctt cttttcgagc agtatgcctg ggcaaaaact
      841 gaaaatagct ggtattgatg tgcattccga taacaataca taccgctgaa atcaaaataa
      901 actgacgttt gaaaaacatc caaaagaaga caaacaaaac attaattaga agcaaatatg
      961 ggaaagctaa gcctaaaaca gatggaatcc aaaactttac agggttaacc tccgacacca
     1021 ggtaagatgc caacaacgcc agtgcaacac aaatattgaa cacaaggata atatttcgga
     1081 ttacttttct caaaaaacca gtgtttatat tgtttccaaa gataaaatgt atcgcttcaa
     1141 ttttcaaaat tacccttaat aactttcaag ctatcttctg ccccttctta caaaaattaa
     1201 aaataatcta ttgatataac atattatctt acaagaggtt ctttctcaag aacaatatta
     1261 tattctaaac aaaaaaaatg cgacctttgc aaaaaattag aataacgatg agaagtaaca
     1321 aaacaggtaa tagccagtta aaaagaaaaa ctaccacacc agcccaaata gctaaaaaaa
     1381 agagcgatag ccgaataaaa tcaaagccag ccaacaaagc tacaagcaaa caggcgggca
     1441 aaggcatcga aaggaacagt cgtgttgaag ttattcccac cggaaaaaca gaaaagcccg
     1501 tagtagaaag aaagaaacca accctttcaa ctccagccaa aagaggcttc cgtcccgatg
     1561 aaaaaattag attgaaccgc ttccttgcta attcaggcgt atgttcacgt cgcgaggccg
     1621 atgtatatat caacacggga tgtgtcactg tcaacgggaa aattaccacc gaactaggca
     1681 ccaaagtctc attaagcgat gatgttcgtt tcaacgggca atctctttgt cctgagaaaa
     1741 aagtttacct gttactcaac aaacccaaag gttacgttac aactaccgat gacccacagg
     1801 agcgcaaaac cgttatggaa ttagttgtca acgcttgccc ggaaagaatt tatcctgttg
     1861 gccggctcga ccgcgatacc acaggattgc tgctctttac caacgacggc gatttggcga
     1921 agaagctaac ccacccaagt cattcccgga aaaaaatata ccaggccacc cttaataaac
     1981 agctaaccaa aaacgacctt ctgtcagttg cccggggtat cgatctcgat gatggtccaa
     2041 ttgcagccga ctcaattaat tatgtcgatg aagaagacaa acgcgaaatt ggcatcgaaa
     2101 tccactcggg ccgaaaccgc attgtacgcc gcattttcga gcatcttggc tacgaggtga
     2161 caaaactcga ccgggtatat tttgccggat taaccaaaaa gaacctgccg cgggggaaat
     2221 ggcgttttct gactcaggca gaaataaatc aattaaaaat gctcaaagtg taattttctt
     2281 gccaaatatg ctacatatcg taaaacagaa tgttcaacac aaagatattt gaaaacaaaa
     2341 aatgtgaaga tcattctgtt tcaagctagt aagaattagt aatttgtgta attattgcaa
     2401 acagagaatg tttatcgggt caattctaat ggtctttttt aagggataat gccagtgaaa
     2461 gatgtgtttc taaatattgt aacccaaaac caagggataa tccacaaagt ttgtggcatt
     2521 tattgtgaca cacatgaaga ccggcaagac ctttttcagg aaattgtatc ccagttatgg
     2581 cgttcattcc caacattccg caacgagtcg aaagtatcga catggatgta tcaggtttcg
     2641 cttaacaccg ccattaccca ttttaaaaag aacaagaaac ggcccgaccg aagcgaaatt
     2701 gggaaaagca tctatcttat tcccgatgaa atttacgacg acacattcga aaaccaggtg
     2761 aaagtgttac acaaagccat tgcaaaatta accgggattg agaaatcgat tgtcctgctg
     2821 tacctcgaag ataaaaagta cgaggaaatt gcagagataa ttggcatcac gcagaattat
     2881 gttcgcgtaa aaatgaaccg gattaagggc aaattaaaaa aactcatggg ggaggaagca
     2941 taatggaatt agaagattta aagaaaacat gggacaaaat ttcttccgaa aacgagaaaa
     3001 gacaactcaa tcaggaagat attgacacgt tactaaagaa ccgtactctt gatattaccg
     3061 agaaaattag acgaaatatt cgaattggac tcattataat tttggcctgg attggacttt
     3121 ggtttgccat tgaaatgctg tttacccccc tgcttgaaaa atttatttca gagcccagcc
     3181 tctctggtga tttggtaaac tggatgacag ccattgaagg gtttacatac ttgctgatta
     3241 ttgctgccat tatcgtcttt ggggttaatt ataaccgcat tgaaaaacaa aacagtactc
     3301 aaatcaacct taagaaaaaa atcaccctgc atatcaggac tctaaatctg tataaaacca
     3361 tgttttatat aatactgata ataatccttt taaacatcac agtcgcattt agtaccggat
     3421 ttatttcagg attcaattct caaactgttg cccagcaact caacatctct aatatttcat
     3481 ttttcaaatg gctgattatt ggtatgtcat ttctgttaac attgggtatt gtaatttcaa
     3541 tttactactt cttgtttcat ttattcttca accgactcta cggcaagtac ttgaaacaac
     3601 taaagaatac tctaaaagaa ttcgacgagg ttaattctct gcaataaaag tcctaagcaa
     3661 attggcttaa tttggttctt cgggaacaaa tactatatct ttgtatcgat attttacaca
     3721 atggggtgcc cataaaaatc ctgcatccag tgtattgaga agataatttc agtgtgtttt
     3781 catctgaagc aaccttctct caacgacatt taagcggggt taaaaattaa gggctgagat
     3841 cataccctct gaacctgatc cggctagtac cggcgaaggg aaggtgtttt caggatgtaa
     3901 attcctgcct cattggttat ttgtttaacc aaaatcttta ttacaatgaa acttttattt
     3961 tcagtattaa tcttactaac cggggtactg ggttttgcca atcaaactta tcgggaagaa
     4021 ttaccacgac cgagaacgaa gctctccccg gagccac
//
LOCUS       DAOPUW010000091         4046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_22369_length_4046_cov_4.355049, whole genome shotgun sequence.
ACCESSION   DAOPUW010000091 DAOPUW010000000
VERSION     DAOPUW010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22369_length_4046_cov_4.355049"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3402)
                     /locus_tag="PKJ43_02475"
     CDS             complement(<1..3402)
                     /locus_tag="PKJ43_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNZ71454.1"
                     /translation="MSQDSLGYIWIATENGASYFDGMNFTTFYHDQSKPGTLASDLVK
                     VIYSDSKGTTWVGTSKGLQIFDHEKNTFRRFSVQDSTETATPYITSILESPDRTKILV
                     SVAALGVVVFDKNTHQLDAQTSDKLAQIYQTRFPGILFFDAKGKLWNFSEQGFFSKVD
                     YNTLTKEPIVWDQKLIGLSKEVVVSTMTEDPVSGNLLIGTFNHGIFIYDRKLGFIRMP
                     KGLTLNNERVRVLLAERREFSSMTPDIWVGSEDHGLKKFDRNKESIYTPDYQYAPVDL
                     ENAKVHSIIQDAQGNIWAGIYQKGLVIIPKLTNSFEYIKLAATEHPMSANIASATGIL
                     HDNQNNLWIGSDGGGLFRIAPDGTKKRFTKDNAQLPNNSVLSLLTDKRGTLWITTYMG
                     GVTTFDNQNGFRLFSNDINLQKANCSTYDPETDNLFFGTLGSGVKVISYPERNIKSFP
                     PNVSSGWVSSVCLDRSGNLWIGETEGLRCYNTLSGEVAHANITGKFTKISLSSLFEDK
                     NGAIWAGSQNGLYFIDVQNEEIRLFTKDDGLPSNSICAIQQDIEGTLWISTMNGLSRF
                     IPSSNTFRKFYAQDGLQDNEFHVKSSWIDKNGKMYFGGINGVSTFNPSDIHEEKKLNS
                     KLYFSSLSVLNHNINYDESLGKKNILDRHISQARTITLKKSQNVFSIGFTLLEYSNPQ
                     KIVYGYMLRGFDNDWRFTSSNHRMATYTNLPSGNYEFCTKAFFDGTHDEQNVVYNNIN
                     IHILPPWYKTWWAYLLYLSIFMITVWEILNIQLRRKIYQKEKAEYEKKELKLQMFTDL
                     THEIRTPFTLVMTPLKSMLQSENDPKKLDMFNLMYRNVLRILRLLNQLMDIRKIDNLQ
                     FKLHFQQSNLIDFIEDLMKSFEQQAILRNIDFRLVSNLESLEVWIDQINFDKVLFNIL
                     SNAFKFTPENGNIMISIDTVKKPLNTSNLGNITEHVEICIENSGSEINENEKERIFDR
                     FYQSDHNKTLGGSGVGLHLAKSIVKLHHGNIQAKNTEKGVAFIIHIPLGNKHLTNEEL
                     EPGKTGESIADIREEEKENTEKELNRLKDPIDAAPKTKRLKHSLVFVDDDADLGKYLR
                     MELASKYNIEVFTNAANAWKLISTTIPDAVVTDLLMPDTDGISLCK"
     gene            complement(3604..>4046)
                     /locus_tag="PKJ43_02480"
     CDS             complement(3604..>4046)
                     /locus_tag="PKJ43_02480"
                     /inference="COORDINATES: protein motif:HMM:NF025862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 97 C-terminal
                     domain-containing protein"
                     /protein_id="HNZ71455.1"
                     /translation="GNNTPNRGSRRITSDVFALATAVLFQSPVQHFALAPNNLTDAPD
                     WAIDFMKKVPNTWDEIKLIDGYPGKYIIMARRSGDKWYVAGINAQSEPLNLKTTLSMI
                     KPGSELKIYSDDQQLNGKVTSTTLSKSQEVNISIPSQGGFLLVD"
BASE COUNT         1063 a          786 c          809 g         1388 t
ORIGIN      
        1 tttgcataat gatatcccgt cagtgtcagg catgagtagg tcggtgacca ctgcatcggg
       61 tattgttgtc gaaattaatt tccatgcatt tgctgcattc gtgaacactt caatgttata
      121 ttttgaggct aattccattc gtagatattt cccaaggtcg gcatcgtcat cgacaaaaac
      181 cagcgaatgt ttcagtcttt ttgtttttgg cgctgcatct atagggtctt tcagtctatt
      241 taattctttt tctgtatttt ccttttcttc ttcacgaata tctgcaatgc tttcgccggt
      301 ttttcccggt tcaagttcct catttgttaa atgtttgttt ccaagaggta tgtgtattat
      361 aaaggctact cctttttcgg tgtttttagc ctgaatattg ccatggtgca gttttacaat
      421 cgatttagca aggtgtaacc caactcccga accaccgagg gttttgttat gatcgctttg
      481 ataaaagcgg tcgaaaatgc gttctttttc gttctcatta atctccgatc ctgaattttc
      541 aatacaaatc tcgacatgtt cggttatatt acccaaatta ctcgtgttga gcggcttttt
      601 taccgtgtcg atgcttatca ttatattccc gttttcgggc gtaaatttga acgcattcga
      661 caggatattg aaaagcactt tgtcgaaatt gatttggtct atccacactt cgagcgattc
      721 aagattactt accaatctga aatcaatatt gcgtaaaatt gcttgttgtt caaacgattt
      781 catgaggtct tcgatgaagt ctatcaaatt agattgctga aaatgtaatt tgaattgaag
      841 gttgtcgatt ttccgtatgt ccatcaattg gttcaaaagc cttaaaatac gtaatacatt
      901 gcgatacatg aggttaaaca tgtcgagttt tttgggatcg ttttccgatt gtagcatcga
      961 tttcaaaggg gtcattacta gggtaaacgg cgtgcgaatt tcatgggtca gatcggtaaa
     1021 catctgaagt tttaattctt tcttttcata ctctgctttt tctttttggt aaatctttct
     1081 tcgtagctgg atattgagta tttcccaaac ggtaatcata aaaattgaaa gataaagcag
     1141 ataagcccac catgttttgt accagggggg aagtatgtga atgttaatat tgttgtaaac
     1201 aacattctgt tcgtcatggg ttccgtcaaa aaaggctttg gtacaaaatt catagtttcc
     1261 actgggcaag ttagtatagg ttgccattcg gtggttcgag ctggtgaaac gccagtcatt
     1321 atcaaaccct ctgagcatat aaccatatac aattttttgt gggtttgaat attcaagaag
     1381 ggtaaagcca atggaaaata cattttgtga tttcttcagc gtaatcgttc gggcttgcga
     1441 aatgtgcctg tcaaggatgt tcttcttccc aaggctttca tcgtagttga tattgtggtt
     1501 taataccgaa agacttgaaa aataaagctt tgagtttagt ttcttttcct catggatgtc
     1561 tgaaggattg aaagtgctta ccccgttgat tcccccgaaa tacattttcc catttttatc
     1621 gatccaagat gattttacat gaaattcatt gtcttgaagc ccatcttgag cgtaaaactt
     1681 tctgaaagtg ttagaagaag ggataaatct ggaaagaccg ttcattgtac ttatccacaa
     1741 tgttccttct atgtcttgct gaattgcaca aattgagttg ctcggtagcc cgtcgtcttt
     1801 cgtgaataat cgaatttctt cattctgaac gtcaataaag tatagtccgt tttgactccc
     1861 tgcccaaatc gccccgtttt tatcttcgaa caacgaactg agtgatatct tagtaaattt
     1921 ccctgtgata ttggcatgtg ctacttctcc cgatagggta ttgtaacagc gaaggccttc
     1981 tgtttcacct atccaaaggt tacctgaacg atcgaggcaa accgaagata cccagcctga
     2041 actaacattc gggggaaacg atttgatgtt tctctcagga tatgatatta ccttaacacc
     2101 actccccagc gtaccaaaaa aaaggttatc ggtttcaggg tcataagtgc tgcagtttgc
     2161 tttttgcaga ttaatgtcgt tagaaaataa cctgaaccca ttctgattat cgaaagttgt
     2221 gacgccaccc atgtaggtcg tgatccaaag tgtacctctt ttgtcggtta aaagactgag
     2281 tactgagttg tttggtagtt gggcattatc ttttgtgaat cttttttttg ttccatcggg
     2341 agctattcta aacaaaccac caccgtcgga gcctatccag agattattct ggttatcgtg
     2401 caaaatgccg gttgcagagg caatgttggc gctcattgga tgttccgtag cggcgagttt
     2461 tatgtattca aaactattag tgagtttggg aataataact aacccttttt gatatatccc
     2521 ggcccaaata tttccttgtg cgtcctgtat aattgaatga actttcgcgt tttccagatc
     2581 tacgggtgcg tattgataat cgggagtgta gatgctttct ttattgcggt cgaacttttt
     2641 cagcccgtga tcttccgacc caacccaaat atcgggagtc atactgctaa attctcttct
     2701 ttcggctaac agtaccctaa ctcgttcgtt atttaaagtc agtcctttag gcattcgaat
     2761 gaagcctagt ttccggtcat aaataaaaat accgtgatta aaggtgccta tcagcaggtt
     2821 accgcttacc gggtcttctg tcattgttga gacaactact tcttttgaca gacctattag
     2881 tttttggtcc caaacgatag gttctttggt aagggtattg taatcgactt tagaaaaaaa
     2941 gccttgttct gaaaagttcc ataattttcc tttggcatcg aaaaatagga ttccagggaa
     3001 acgtgtttga tatatctggg ccagtttatc tgatgtttga gcatctaatt gatgcgtgtt
     3061 tttatcgaaa acaacgacac ctaaagcggc tactgaaacc agaatctttg ttctgtcagg
     3121 actttctaag atcgatgtaa tataaggcgt tgctgtttct gtagaatctt gaacagaaaa
     3181 tcttctgaag gtgttttttt cgtggtcgaa aatttgcaag cccttcgatg tcccaaccca
     3241 ggtcgttccc ttgctgtcgg aataaatgac cttcactaag tcactggcta gtgttcctgg
     3301 tttgctttgg tcatgataaa aggtggtaaa attcatacca tcgaaatagc ttgccccatt
     3361 ttctgtggca atccaaatat atcctaaact gtcttgcgac actttgtgta ttaggctgtt
     3421 gggtaagccg ctttctgaat tgtaaaatcg tattttttgc ccaaaaccgg gtacagtaat
     3481 taatagagcg aaaagtatct gaagaaagat caaatactta ttcagtctac attttgtatg
     3541 tttcaaaaat acccccacct tttacttgta tcaattcaat attatcctta gaaaagacaa
     3601 ggtttaatca actaataaaa aaccaccttg actagggatt gatatattaa cctcttggct
     3661 tttgctcaat gttgtactag ttactttccc gtttaattgc tggtcatcac tatatatttt
     3721 aagctctgaa ccgggtttga tcatagataa agtagtcttt aaattcaatg gttccgattg
     3781 tgcatttatt ccggcaacat accatttgtc cccgcttctt ctggccatga taatatattt
     3841 tccggggtat ccgtcaatga gtttaatttc gtcccaagta tttggtactt ttttcataaa
     3901 gtcgattgcc caatcgggag catcggtgag gttgttgggt gccagtgcaa aatgctgaac
     3961 cgggctttgg aacagcacgg ctgtggctag tgcaaacaca tctgatgtta ttcttctcga
     4021 gccgcgattg ggggtattat ttccat
//
LOCUS       DAOPUW010000092         4032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_22490_length_4032_cov_3.230827, whole genome shotgun sequence.
ACCESSION   DAOPUW010000092 DAOPUW010000000
VERSION     DAOPUW010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22490_length_4032_cov_3.230827"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2165
                     /locus_tag="PKJ43_02485"
     CDS             <1..2165
                     /locus_tag="PKJ43_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNZ71456.1"
                     /translation="RWGRGQETYGEDPYLTSRMGVQVVKGLQGPEDAKYRKLLACAKH
                     YAVHSGPEWSRHELNLNNVNPRLFSETYMPAFKALVQEADVRQVMCAYQRLDDEPCCG
                     STRLLQRILRDEWGFKHIVVSDCGAITDFYTSHKVSSTPVHAASKGVLAGTDVECVWE
                     NYPYKTLPEGVEKGLIKMEDIDKSLMRILIGRFELGDFDDDALVPWAQIPASVLNCDK
                     HKQLAYKMAQQSMTLLQNNNNILPLSKSSKKIAVIGPNAADEEMLWGNYNGTPIHTIS
                     ILNGIKSKISESKIIYDKGCDLVEDKVTESYFSQLSFDGKPGFKATYWNNPSREGNSV
                     ISQQIASAIKKTTAGQHEFASGVKLEGFSALFQAEFTPKTTEELVFKGGATGYFELLV
                     NGEQLQMYNNWRTLPSRIAYKFEAGKKYIIEMRYAQLNNWQANIEIDFGKEVDVDYTD
                     LINKLKGIETVVFVGGLSGKLEGEEMPVSYPGFKGGDRTDIELPAVQRNALKALKAAG
                     KKVIFVNCSGSAIALEPETQSCDAILQAWYAGEQGGKAVADVLFGDYNPGGKLPISFY
                     KNSDKLGDFEDYSMTDRTYRYTTDYLFPFGFGLSYTTFEIGNASISKTSIKANENVKL
                     TIPVKNTGKRAGTEIVQVYIRKMNDLEGPLKTLRGFQRMELAAGKSETATIELTPASF
                     EFYDWSQRQMAVTPGEYEVLYGNSSDAKDLKMLKVTIL"
     gene            2187..3098
                     /locus_tag="PKJ43_02490"
     CDS             2187..3098
                     /locus_tag="PKJ43_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71457.1"
                     /translation="MKKILISLAVLVLTGIGFQKSLLAQETGTTEKTTSRVVEDGGTG
                     AYSAIMSTDASLTTHTIFRPKDLSAFGKKEKLPIIAWGNGACANSPWEHINFLSEVAS
                     HGFLVIAIGPMPQEGQRGGGRSTSSQLIDAIDWAIAQNNNKDSELYGKIDISKIAVSG
                     MSCGGLQTLETAPDPRITTAIICNSGIIGDGGGMPGMPGLKKDHLEKLHTPTLYLLGG
                     ETDIAYKNGMDDFSRINHVPVFVANMDVGHGGTYSQPHGGEFAKVATAWYKWQLKGDK
                     EAGKMFTGDPCELSKSTVWKVEKKNLK"
     gene            3437..>4032
                     /locus_tag="PKJ43_02495"
     CDS             3437..>4032
                     /locus_tag="PKJ43_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNZ71458.1"
                     /translation="MGAAVCTAICLSAQNPIVQTYFTADPAPMVYDGTVYLYTSHDED
                     STVRNFFTMNDWKCYSTTDMVNWTDRGTVLSYKTFDWSRGDAWAGQCIFRNGKFYYYL
                     PVNQKNGGNAIGVAVSDSPTGPFKDALGKPLLSGYGYIDPTVFIDDDGQAYLYWGNPN
                     LWHVKLNNDMVSYDQEYGIKKEDLKDENFGYRSKKIDN"
BASE COUNT         1219 a          853 c          938 g         1022 t
ORIGIN      
        1 cgcgctgggg acgtggtcag gagacttacg gcgaagaccc ttaccttact tcgcgcatgg
       61 gcgtgcaggt ggttaaaggg ttgcaaggtc ccgaagatgc caagtaccgc aaattgcttg
      121 cctgtgcaaa acattacgcc gttcactcgg gacccgaatg gagccgccac gagttgaatc
      181 tcaataatgt aaacccacgc ttgttcagtg aaacctatat gcctgccttc aaagcattgg
      241 tgcaagaggc tgatgttcgt caggtaatgt gcgcctacca acgtttggac gacgaacctt
      301 gttgcggaag tacccgtttg ttgcaacgca tcctgcgcga tgagtggggc ttcaaacaca
      361 tcgttgtgtc cgactgtggt gccataaccg acttttatac ttcgcataaa gtatcttcaa
      421 ctccagtaca tgctgcatcg aaaggcgtgt tggccggaac tgatgtagaa tgtgtatggg
      481 aaaattatcc gtacaaaacc cttcccgaag gtgttgaaaa aggactgata aaaatggaag
      541 acatcgacaa aagtttgatg cgcattttga taggccgttt cgagttgggc gatttcgacg
      601 acgacgcatt ggtgccttgg gcgcaaattc ctgcttcggt gttgaactgc gacaaacaca
      661 agcagttggc ctacaaaatg gcgcaacaaa gcatgacctt gcttcaaaac aacaataata
      721 ttttgccgct gtcgaaatca tcgaaaaaaa ttgcggtgat tggccccaat gctgccgacg
      781 aggagatgtt gtggggcaat tacaacggca cgcccattca tacaatttct attttgaatg
      841 gcatcaaatc gaagatttct gaaagcaaaa tcatctacga caaaggctgc gacttggttg
      901 aggacaaagt gaccgaaagc tacttttcgc aactgtcgtt cgacgggaaa cccggcttca
      961 aagcaaccta ttggaacaat ccgtctcgtg aggggaattc cgttatttca caacaaattg
     1021 caagtgccat taagaaaaca acagccggtc agcacgaatt tgcttcgggc gtgaaactcg
     1081 aaggtttttc agcactattt caagctgaat tcaccccaaa aaccaccgaa gaattggttt
     1141 tcaagggagg cgcaacggga tatttcgaat tgctggtgaa tggcgaacaa ttgcagatgt
     1201 acaacaattg gcgcacgctg ccatcgcgca ttgcctacaa gttcgaagct ggcaaaaagt
     1261 acatcatcga aatgcgttat gcccaactaa acaactggca agccaacatt gaaattgact
     1321 tcggaaaaga ggttgatgtt gattatactg atctcattaa caaattgaaa ggcatcgaaa
     1381 ccgtggtgtt tgtgggcgga ctttcgggca agctcgaagg cgaagaaatg cccgtttcgt
     1441 atcccggttt caaagggggc gaccgtaccg acattgaatt gcctgccgtt cagcgcaacg
     1501 ctttgaaagc attgaaggct gccggtaaaa aggtgatttt tgtaaactgt tcgggctcgg
     1561 ccattgcctt ggaacccgaa acacaaagct gcgacgccat tttgcaagcc tggtatgctg
     1621 gcgaacaagg cggtaaagca gttgccgacg tgctgtttgg cgactacaac cccgggggaa
     1681 aacttccaat ttctttttac aaaaactcag acaaactggg cgatttcgaa gattactcca
     1741 tgaccgaccg cacctatcga tacacaaccg attacttgtt cccattcggt tttggattga
     1801 gctacaccac gttcgaaatt ggcaatgcaa gcatcagtaa aacaagcatc aaggccaatg
     1861 agaatgtgaa gttgaccatt cctgtaaaaa atacgggcaa acgtgccgga accgaaattg
     1921 ttcaggtcta catccgcaag atgaacgact tggaggggcc gctcaaaaca ttgcgtggat
     1981 tccaacgtat ggaattggca gccggaaaat cggaaacagc caccatcgaa cttacgcccg
     2041 catcgtttga gttctacgac tggagccaac gccaaatggc tgttacaccc ggcgaatacg
     2101 aagttttgta cgggaacagt tcagacgcaa aagatttaaa aatgttgaaa gtaactattc
     2161 tataaaataa ttaaaccatt tttagtatga agaaaatact cattagtctt gctgttctgg
     2221 tacttactgg aatcggtttt caaaaatcat tgttggctca ggaaactgga acaactgaaa
     2281 aaacaacttc acgggttgtt gaagatggtg gtaccggagc gtattctgcc attatgagca
     2341 cggacgcttc actgacaacc cacaccattt ttcgacctaa agatttaagc gcttttggaa
     2401 agaaagaaaa gttaccaatc attgcctggg gtaatggtgc ttgcgccaat tctccctggg
     2461 aacatatcaa tttcttgtca gaagtggcat cacacggttt tttggtaatt gccattggcc
     2521 caatgcctca ggaaggccag cgtggtggtg gccgttcaac gtcatcgcaa ctgattgatg
     2581 ctattgactg ggccattgct cagaacaata ataaagacag tgagctttac ggcaaaatcg
     2641 atatttcgaa aattgctgtt agtggtatgt catgtggcgg tttgcaaaca ctcgaaacag
     2701 ctcctgatcc aagaataaca actgctatta tttgtaatag cggtatcatt ggagatggcg
     2761 gtggaatgcc cggaatgccc ggtttgaaaa aggatcattt agaaaaactg catacgccaa
     2821 cgctctattt gttgggtggt gaaactgata tagcctataa aaatggcatg gacgacttca
     2881 gtcgcatcaa ccatgtgcct gtatttgtag ccaatatgga tgtgggtcat ggtggaacat
     2941 acagccaacc tcacggtggt gagtttgcta aagtggcaac tgcttggtac aaatggcaat
     3001 tgaaagggga caaggaagcc ggaaaaatgt tcaccggaga tccatgcgaa ctttcaaaat
     3061 caacagtttg gaaggtcgaa aagaagaatt tgaagtagtt atttataaaa aacgatttcg
     3121 tcttatatag aatcaattct tagatcaatg catctctgcc agaagacgga ttggaattat
     3181 taaataaaaa aatgcaagta gatgagggct ggtggctcag ccatcagccc tcatttcata
     3241 aaagaaagat cacattatta aggtagtaag tatttcaaat atttttaaca ccaaaaagaa
     3301 ctgtcatcat actcgcgtca aatttttaac ttgatacccc atgctgaaat ttgtaacgta
     3361 ttaataagta acagaaactg attagaaaac agaaataata aaagaaacta tgaattcatt
     3421 ttgtaaatta tcactgatgg gtgctgcagt ttgtactgca atttgtctta gcgcgcaaaa
     3481 ccccatcgtt caaacctatt tcaccgccga ccctgcacca atggtgtacg atggtacagt
     3541 ttatttgtac acttcgcacg acgaagattc aacggtgcgg aatttcttca ccatgaacga
     3601 ttggaagtgc tattccacaa ccgacatggt aaattggacc gatcgaggaa cggtattgtc
     3661 gtacaaaacc tttgattggt cgcgtggcga tgcttgggct ggtcagtgca ttttccgtaa
     3721 tggaaaattc tactactatt tgcctgtgaa ccaaaaaaat ggtggaaatg ccattggagt
     3781 tgcggtttcc gacagtccaa ccggcccttt caaagatgct cttgggaaac cactcttaag
     3841 tggatacggg tacattgacc caaccgtttt catcgacgat gacggacaag cctatttgta
     3901 ttggggaaac cccaacttat ggcacgtaaa attgaacaac gatatggttt catacgatca
     3961 ggaatatggt atcaagaaag aagatttaaa agatgaaaat ttcggatacc gttcaaaaaa
     4021 aattgataac ca
//
LOCUS       DAOPUW010000093         4024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_22539_length_4024_cov_3.563870, whole genome shotgun sequence.
ACCESSION   DAOPUW010000093 DAOPUW010000000
VERSION     DAOPUW010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22539_length_4024_cov_3.563870"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..396
                     /locus_tag="PKJ43_02500"
     CDS             139..396
                     /locus_tag="PKJ43_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNZ71459.1"
                     /translation="MSKCLINNNIRTLRFMAGEMTQQELADRTGVTRQTIVAIENGKY
                     SPTLELAFRISHVFNKPLNEVFSYQYDEDPILTKKTTPKCK"
     gene            401..1261
                     /locus_tag="PKJ43_02505"
     CDS             401..1261
                     /locus_tag="PKJ43_02505"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HNZ71460.1"
                     /translation="MTTLTVKTQTKKLNPRQVFWKCGACSHAMFHLLNNEFDNLKPIE
                     EKASDMLAGGIAQKGEQCGMLWGGSLAIGTEAYRKFENKDQAIASAIHASRLLLNSFY
                     NRNKTVNCRDISKVDWENKFQMAFYVVKTFAQGFVFSPCFNLIVKWTPEAVEAARTGL
                     EENISYTSPCISCASKVVKEMGGSEEESVMAAGFAGGIGLSGNACGALAAAIWYKMLV
                     WERNNQGKSQTFFDNPDAKRVMRAFYIKTDSEMLCKNICKRKFDTIDEHSEYIRNGGC
                     KNIIDALAKA"
     gene            1264..2412
                     /locus_tag="PKJ43_02510"
     CDS             1264..2412
                     /locus_tag="PKJ43_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNZ71461.1"
                     /translation="MISNILKRNVYLDNNATTRVSKAVRKTMNSVLKYHWGNPSSGYQ
                     KGKTANSIVENAREQVAGAIHAHPHEIYFTSCATEANNMVLKTLSNHFYPAKKKIIST
                     PIEHPSVINTLNFLETKGIIVQYCPVDRYGFVSTEELEKLIDNDTFLICCMLANNEIG
                     TIQNIREISSLAQKHGILVMSDCVQAFGKIPIDVKDLGIDYATFSAHKLYGPKGIGCL
                     YAKISSPIEAFIHGGHQEEGLRAGTESVHNIAGFGKACQATKQLISHSNQTRLLKEHF
                     IQQLRIIKPDIVINSPTTDCLPNTISITFPNISSRELMMMLDYNGISVSSGSACSSQL
                     NKPSHVLKAIGLSDTLANETLRISFGKDTRKGDIDYTVKTIRKYIKEQ"
     gene            complement(2482..3387)
                     /locus_tag="PKJ43_02515"
     CDS             complement(2482..3387)
                     /locus_tag="PKJ43_02515"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71462.1"
                     /translation="MEIKELSNKLVEAYSDQNLNKITLTLLNLYKEQQYAKLNKIAEL
                     IADFLVVEINDTGKGFSKLMMLYHPDRGDVHRKEIVELEKKGDYDGLLGFSHIFKLMR
                     VDEIAERLESYEDIDYSPVYEWDWDSKADSFSVKDEEPEKDFKPRKQRSPKTYSFYDA
                     FRIRIFGNTKTEIPYYYFDDIDEIELSESAIDNLDGIERCRHAVTVDLSGNKILDLTQ
                     LWGLSLIEELSLSGNCIELIDSLANLTNLRYLNLSDNKVRDLSPLFDLGKLETVILHG
                     NPVNQQQIEELEELGVEVEWNLQKR"
     gene            complement(3605..>4024)
                     /locus_tag="PKJ43_02520"
     CDS             complement(3605..>4024)
                     /locus_tag="PKJ43_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71463.1"
                     /translation="QCARNENHNLSYLFAGFQHNYFNIVLTNLLLTIIVVAGFLFLIL
                     PGIFLAIRLAFVPYLVVDKELEPMKAIETSWKMTSGYEFTILGMALMSIFIVIIGLIC
                     LVVGVFPAIIWINSSFASLYQAVLSKIKENSEVSIEI"
BASE COUNT         1317 a          833 c          777 g         1097 t
ORIGIN      
        1 ggtttatctg tgccatgggg gtattagcct ttggtgcaaa accttcggcc atgttcatca
       61 tcctgctggc gtccggattt ggttctgcaa ttatttccga actggtgaaa ctatattact
      121 acagaaaggg ggtttaagat gtccaaatgt ttaataaata ataatatacg aacactgcgt
      181 tttatggccg gcgaaatgac ccaacaggaa ctggctgacc gtaccggggt tacacgccaa
      241 accatcgtgg ctatcgaaaa cgggaagtac tcacctaccc tcgaactggc cttccgtata
      301 tcgcatgtat tcaacaaacc acttaacgag gttttttcat accaatatga cgaagacccg
      361 atcttaacca agaaaacaac accaaaatgc aaataatacc atgacaactt taaccgtgaa
      421 aacacaaaca aaaaaactaa atcccagaca ggttttctgg aaatgcggag catgctccca
      481 tgccatgttt catttattga acaatgagtt cgacaactta aaacccattg aagaaaaggc
      541 ttctgatatg ctggccggag gcattgccca aaaaggagag caatgcggaa tgctgtgggg
      601 aggctcactg gcaataggaa ccgaggccta ccgaaaattt gagaataaag accaggcaat
      661 agcttcagcg attcatgcat ccaggctgct gttgaattcg ttctacaaca ggaacaaaac
      721 agtaaactgc agggacatat cgaaagtcga ctgggaaaac aaatttcaaa tggctttcta
      781 cgtagtaaaa acctttgccc agggttttgt tttcagcccc tgttttaacc tcattgttaa
      841 atggacgccc gaagcggtcg aggcagcaag aaccgggttg gaagaaaata tcagctacac
      901 atcgccatgt attagttgcg caagcaaagt agtgaaagaa atgggaggat ctgaagaaga
      961 gtcggtgatg gcagctggct ttgccggggg aattggcctc agcggaaatg cttgcggtgc
     1021 cttggcagct gcaatttggt acaaaatgct ggtttgggag agaaacaatc agggaaagtc
     1081 gcaaacgttt ttcgacaacc cagatgccaa aagagtgatg agagcttttt acataaaaac
     1141 cgactcggaa atgttgtgta aaaacatctg caaaagaaaa ttcgacacga tcgacgagca
     1201 ctcagaatac atcagaaacg gtggctgcaa aaacattatt gacgccttag caaaagcata
     1261 aacatgatat caaacattct taagcgaaat gtctatctcg acaataatgc tacaacccgt
     1321 gtgagcaaag cggtaagaaa aaccatgaac agtgtattaa aataccattg gggcaatccg
     1381 tcatcgggct accaaaaagg aaaaacggcc aacagtattg ttgagaacgc aagagaacaa
     1441 gtagccgggg ctattcatgc ccatccccac gaaatatact tcaccagttg cgctactgaa
     1501 gccaacaata tggtattgaa aacgttaagt aatcatttct atcctgcaaa aaagaaaata
     1561 atctctacgc caatcgagca cccttcggta ataaatacac tgaatttttt agaaaccaaa
     1621 gggataattg ttcaatactg tcctgttgat cgttatggtt ttgtttcaac agaagaattg
     1681 gaaaaactga tcgacaacga tacattttta atttgttgta tgctcgcaaa taacgagatc
     1741 gggacaattc aaaacattcg tgaaatttca tcacttgccc aaaaacacgg catccttgta
     1801 atgtccgatt gtgtgcaggc atttggtaaa attcccattg atgtaaaaga cctggggatt
     1861 gattatgcta cattctcggc acacaaactc tatggaccaa aaggaattgg gtgcctgtat
     1921 gcaaaaataa gcagtcccat cgaagctttc atccatggag ggcaccagga agaaggcctg
     1981 cgggctggca ccgaatcggt tcataatatt gccggatttg gaaaagcttg ccaagctaca
     2041 aagcaattaa tctcgcattc aaaccaaacc cggttgctta aggaacactt catacaacaa
     2101 ttacgcatta ttaaacccga cattgtaata aattcaccaa caacagattg cctgccaaat
     2161 acaatcagca taacttttcc aaacatcagt agccgggagt tgatgatgat gctcgattat
     2221 aatggaattt ctgtttcgtc gggctcggcg tgtagctcac agcttaataa accatcgcac
     2281 gtcctgaaag ccatcgggct aagcgatacg ctggccaatg aaaccttaag gatcagtttt
     2341 ggaaaagata cccggaaagg cgatatcgac tatacggtaa aaacaatcag gaaatacatc
     2401 aaagaacaat aaatgaacaa cttaatattc aagacacttg atatgaactt cgtatttttc
     2461 ctattgacaa actattaggc attatctttt ttgaagattc cattctacct caacaccaag
     2521 ttcttctaat tcctcaattt gttgctggtt caccggattt ccgtgtaaaa tgaccgtttc
     2581 tagctttcca agatcgaaaa gcgggctcag gtctcttact ttgttgtccg acagattgag
     2641 ataccgcaga tttgtaaggt ttgccaggct atcgataagt tcgatacaat ttcccgagag
     2701 gctgagttct tcaataagcg acagccccca taattgagtc aaatccaata ttttgttacc
     2761 cgacaaatcg actgtcacag catgcctgca acgttctatt ccatcaaggt tatcgattgc
     2821 agactcagaa agttcaatct catcaatgtc gtcgaaatag taataaggta tttctgtttt
     2881 agtgttacca aaaatcctaa tacgaaatgc atcataaaaa gaatacgtct ttggtgaacg
     2941 ctgttttctt ggtttaaaat ccttttccgg ctcttcgtct ttaaccgaaa aagaatcggc
     3001 cttgctatcc caatcccatt cataaacagg agagtaatca atatcttcgt agctttccag
     3061 gcgctcggcg atttcatcaa cccgcatcaa tttgaaaata tgggaaaacc caagcaaccc
     3121 atcgtaatca ccctttttct ccaattcaac aatttctttg cggtgaacat cgcctctgtc
     3181 gggatggtaa agcatcatca atttcgaaaa tcccttacca gtatcgttaa tttcaactac
     3241 aagaaaatca gcgattagtt ctgcaatttt attcaatttt gcatattgtt gctccttgta
     3301 taggtttagc aaggttaggg taattttatt aaggttttga tcagaatatg cttcgaccag
     3361 tttgttgctc aactctttta tttccatagt aatactaatt ttatttgggt agaatgattt
     3421 ccggaaaata ttgcttattc aattttcgtt ttttagataa tgaacttatt gtcatcgaaa
     3481 aaaattcaga cgatgaaaaa atcttcaccg tgcaataaag attatggcag atttttgatg
     3541 tttgtatatc tctttaaagc aaagcgttgc ttgtaaaaaa tcttataatt agttacccta
     3601 tcaattaaat ttcaattgaa acttctgaat tttcttttat cttgctgagt acagcctgat
     3661 ataaactggc aaatgatgag tttatccaaa taattgctgg aaaaacaccc acaaccagac
     3721 aaatcaagcc aataatcaca ataaagatgc tcattagtgc catacccaag atggtaaatt
     3781 cgtatccgct ggtcattttc caactggttt caattgcttt cataggttca agttccttat
     3841 caacaaccaa ataaggaaca aaggctaagc ggattgccag aaatattcca ggtaaaatca
     3901 gaaacagaaa accagccaca acaataatgg tcagtaataa gttcgtaagc actatattga
     3961 agtaattgtg ctgaaaacca gcaaataaat agcttaagtt gtgattttca ttccgggcac
     4021 attg
//
LOCUS       DAOPUW010000094         4015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_22610_length_4015_cov_3.827525, whole genome shotgun sequence.
ACCESSION   DAOPUW010000094 DAOPUW010000000
VERSION     DAOPUW010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22610_length_4015_cov_3.827525"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PKJ43_02525"
     CDS             complement(<1..242)
                     /locus_tag="PKJ43_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNZ71464.1"
                     /translation="MDQLLTLAIQAAVAAGNEIMKVYNEADFEIELKSDNSPLTKADK
                     AAHQIITSYLANTLPILSEEGKAIPYEIRKDWGKYWL"
     gene            complement(650..1153)
                     /locus_tag="PKJ43_02530"
     CDS             complement(650..1153)
                     /locus_tag="PKJ43_02530"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HNZ71465.1"
                     /translation="MTKSWYVVYTRSRAEKKAYAELLALEIEAYLPTQRKLRQWSDRK
                     KWVDMPLFPGYIFVHITNKDYDKTLKINNIVSFVRFEGKAAIVSNSQIESIKQIANQT
                     IHEVDISHQLFNEGDQVQVTSGPLIGLKGSLISYRGKKRVAVIINQLSISLLVEINVD
                     SLEKIKE"
     gene            1406..2470
                     /gene="dinB"
                     /locus_tag="PKJ43_02535"
     CDS             1406..2470
                     /gene="dinB"
                     /locus_tag="PKJ43_02535"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNZ71466.1"
                     /translation="MIRKIIHIDMDAFYASVEQRDHPELRGKPVAVGGNSRRGVLATA
                     SYEARKYGVKSAMPTSMALSLCPDLIVIQSNGKAYQEASQVIRSIFYEYTDWVEPLSL
                     DEAFLDVTFCDVAGRSATLIAAEIKKRIKEETALTASAGVSYNKFLAKVASDYKKPDG
                     LFVIPPENALAFLEELPIGKFFGVGKVSAQRFSTMGVKNGKDLKALSRENLVSCFGKA
                     GAYYYDIVRGIDNREVIPFRESKSIGAECTFETDIFEVTDIESRFVAIAERAWRRVNK
                     QNVEGRTITIKIKFYDFEIFSRSKTFDHFIEDKEMFIFEAVQLLQKEYPLQKKVRLLG
                     VTLSNFNEESMEPVQSILKF"
     gene            2482..>4015
                     /locus_tag="PKJ43_02540"
     CDS             2482..>4015
                     /locus_tag="PKJ43_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HNZ71467.1"
                     /translation="MEEKIHQLVAAKVNISLHQVKNTIMLLNEGATIPFISRYRKELT
                     GSLDELSIAMIQKEYNYFVELGKRKQTILKTIGDQGVLTPELQKQIEETYDSSTLEDI
                     YLPYKPKKRTRATIAREKGLEPLAKIMMGQFEWDIEKKAKSFLNENVDTVDDALAGAR
                     DIVAEWVSENQRARDVVRYMFKRGAVIESKVVKGKEADGQKYRDYFNWSEPLSRCPSH
                     RLLAILRASKEDFVKITVSVDEEEAVSRLDGIFLRGNTSCKNHVKLAVHDSFKRLIAP
                     SIETETMQTMKEKADLEAIKVFGENLRQLLLAAPLGQKRVMAIDPGYKSGCKLVCLDE
                     QGNLLHNETIYPHPPQREVRESAKKLGTLVSAYKIEAIAIGNGTAGRDTEQFIKYLKF
                     DRDLTLFSVNEDGASVYSASKVARDEFPEYDVTVRGAVSIGRRLMDPLAELVKIEPKS
                     IGVGQYQHDVDQKMLQENLDRVVESCVNMVGVHVNTASKHFLNYISGLGPELAQNIVD
                     FRR"
BASE COUNT         1200 a          694 c          864 g         1257 t
ORIGIN      
        1 agccagtatt tgccccagtc ttttcgtatt tcatacggaa tggctttccc ttcttcgctt
       61 agtattggta aagtatttgc taaatacgat gtaattattt ggtgtgcagc tttgtctgct
      121 tttgttagtg gcgaattgtc tgatttaagt tcaatttcga agtcggcttc gttataaact
      181 ttcattatct cgtttcctgc tgcaacggct gcctgtattg ctaaagttag aagttgatcc
      241 atgtttttgg tttgttctgg ttactcacag cttcgcttcc tcagtcacaa agatggctaa
      301 gcttgagaaa ttaaatctga caatcaatag catattatgt cgcgatattg tcaattttgc
      361 tttttttacc taattcgtta ttatctaaat gcttgtcaat tctcaaaatt caatttaaag
      421 aattcagaga aatacaactg tttgctattt ttatcaaaat cgtgcaaata agcaatcttt
      481 cttatcgacg tgatcaggtt tttgagcatg gtttgagtaa tagatactca tttcggtcaa
      541 aagtaaatat tccgatgaat aatgccaaat gtagaaaata cctgtatttg gtagttcgag
      601 caatgttgag attaattagg ctttatgaac gaaagcgggt atgttgtttt tattctttta
      661 ttttttcaag agaatcgaca ttaatttcaa ctaatagaga aatacttaac tgattgataa
      721 ttactgcaac gcgtttcttt cccctgtatg aaatcaatga accttttaat ccaattagag
      781 gcccggaagt tacctgaact tgatcccctt cattaaataa ttggtgtgaa atgtcgactt
      841 catgaatggt ttgattggca atttgtttaa tcgattcaat ttgggaattt gagacaattg
      901 ctgctttgcc ttcgaatctt acaaatgata ctatattatt tattttaagt gttttatcgt
      961 aatctttatt ggttatgtga acaaaaatat atccaggaaa caagggcata tcaacccact
     1021 ttttgcggtc gctccactgt cttaattttc gttgggttgg caaataggct tctatttcga
     1081 gagcaagtag ctcagcataa gcttttttct cagctcttga acgagtgtaa acaacatacc
     1141 agctttttgt catgacaaaa gaacgttatt ttaatattag aataatgcac attttcagtt
     1201 ttttagctaa taatagagtg cattgttttt tttggctaaa gctattaaaa gtttaataat
     1261 tcaaaaacag ttacaagtaa aataccatat cgcctatgtt aattatggtt tgcttttcta
     1321 tactggataa gatttatctg cgatgtcgtg tttctagaaa gacatatcca aattttgtat
     1381 ttttggacaa atattaattg gcattttgat tcgtaaaata attcatatag acatggatgc
     1441 tttttatgca tcggttgagc agcgtgatca tcccgaattg aggggtaagc ccgtggctgt
     1501 tggaggaaat tcgcgccggg gtgttcttgc gacagcaagt tatgaagctc gcaaatatgg
     1561 tgttaaatcg gctatgccta cctctatggc cttatcgttg tgtcctgatc ttattgtaat
     1621 tcagtcgaat ggtaaagctt atcaggaggc atcgcaagta attcgttcaa ttttttacga
     1681 atataccgat tgggtagagc cgctttccct tgatgaagct tttctcgatg taactttttg
     1741 tgatgttgca ggcagatctg ctaccttgat tgctgctgaa attaaaaaac gaataaaaga
     1801 ggaaactgcg ttaactgctt cagcgggtgt gtcgtataat aagtttcttg caaaagttgc
     1861 gtccgattat aaaaaacccg acgggctctt tgttatcccg cctgaaaatg ctttggcttt
     1921 tttggaagaa cttcccattg gtaaattctt tggggtggga aaggtctcag cgcagcgctt
     1981 tagtaccatg ggggttaaaa acggtaaaga cctcaaagcc ctatcccgcg aaaatctggt
     2041 cagttgtttc gggaaagctg gagcatatta ttacgatatt gtgcggggta tcgacaatcg
     2101 tgaagtgatt ccctttcgcg aaagcaaatc aattggtgcc gaatgcacgt ttgaaaccga
     2161 tatttttgaa gtgactgata tcgaatcccg ttttgtcgca atcgcagaaa gggcttggcg
     2221 cagagttaat aaacaaaatg tagaaggaag aacaattaca ataaaaatta agttttacga
     2281 ctttgagatt ttttctcgca gtaaaacctt cgatcatttc attgaagata aagagatgtt
     2341 tatttttgaa gccgttcagc ttcttcagaa agaatatccg ttgcaaaaaa aagtacggct
     2401 tcttggggtt actttgtcaa atttcaatga agaaagtatg gaacctgttc aatcaatatt
     2461 aaagttttaa taatcattac gatggaagaa aaaatacatc agttggttgc tgccaaagtg
     2521 aatataagcc ttcaccaagt gaagaacaca ataatgttgc ttaacgaagg agccacaatc
     2581 ccttttattt cgcgttatag aaaggaactc accgggagcc tcgacgaact gagcattgcc
     2641 atgatccaga aggaatacaa ttattttgta gaactcggca agcgaaagca aaccatcttg
     2701 aaaaccattg gagatcaagg ggtacttact cccgaattac aaaagcaaat tgaagaaacc
     2761 tacgattctt ctacactcga agatatttac cttccataca aacccaagaa acgaacaaga
     2821 gctaccattg cccgagagaa aggtcttgag ccccttgcaa aaattatgat gggacagttt
     2881 gaatgggata ttgaaaagaa agcgaaaagc tttctgaacg agaatgttga tacggtagat
     2941 gatgccctgg caggtgcacg cgatattgta gccgagtggg tcagcgaaaa tcaacgggcc
     3001 cgcgatgtgg ttcgctatat gtttaaacgt ggtgctgtaa tcgaatcgaa agtcgtaaag
     3061 ggaaaagagg ccgatggtca gaaatatcga gactacttta attggagcga accactaagt
     3121 cgttgtccat cacatcgctt attggccata cttcgtgcat caaaagaaga ttttgttaaa
     3181 attacggttt cggtagatga agaagaagct gtgagcaggc tcgatggtat ttttcttcgt
     3241 ggaaacacat catgcaaaaa tcatgtaaaa ttagctgtgc atgatagttt taagcggctc
     3301 attgccccgt ctattgaaac tgaaactatg cagacaatga aagaaaaggc agatcttgaa
     3361 gcaataaaag tgtttggtga aaacctaagg cagttgttac tcgctgcacc tttggggcaa
     3421 aaaagggtga tggccataga tccgggatac aaatcgggtt gcaaattggt gtgccttgac
     3481 gaacagggaa atcttttgca caatgaaact atctatccgc atccgccaca acgtgaagtg
     3541 cgcgagtctg ctaagaagct gggaacattg gtgagtgcct ataaaattga agctattgca
     3601 ataggaaacg gaactgcagg tcgagatacc gagcagttta tcaaatactt gaagtttgat
     3661 agggatctta ccctcttttc ggtaaacgaa gatggggctt cggtatattc tgcatcgaag
     3721 gttgcccgtg atgaatttcc ggaatacgat gttacggtgc gaggcgctgt ttcaattggc
     3781 cgccggctga tggatcctct tgctgagttg gtgaaaatag aaccgaaatc gataggtgta
     3841 gggcaatacc agcatgatgt tgaccagaaa atgctgcaag aaaatctcga tcgtgtagta
     3901 gaatcgtgtg taaacatggt cggtgtacat gtaaatactg caagtaaaca ttttttgaat
     3961 tatatatcgg gtttggggcc tgaacttgct cagaatattg ttgattttcg tcgtg
//
LOCUS       DAOPUW010000095         4003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_22711_length_4003_cov_3.488349, whole genome shotgun sequence.
ACCESSION   DAOPUW010000095 DAOPUW010000000
VERSION     DAOPUW010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22711_length_4003_cov_3.488349"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="PKJ43_02545"
     CDS             <1..1494
                     /locus_tag="PKJ43_02545"
                     /inference="COORDINATES: protein motif:HMM:NF039744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6377 domain-containing protein"
                     /protein_id="HNZ71468.1"
                     /translation="TYFNQKNERIKKLTTSLKQTEKNGQTTAAFSLCSELIDEYKSFD
                     FDSASKYVTHLNSLAYKLGDKEKIAQAKIKSGFTLLSSGMFKESLDSLLSIDSEKLPE
                     SLKPEYYNVIARTYYDLADYNSDDYYSSIYRKLGTAHLDSALALLDKNSSTYWASMGL
                     RRMKADDYEGAADAFNYLITKFDITDHTYAIATSSLGYLYTLMGRDNEAIDMLIKAAN
                     ADIVSSTKETVALRNLAVHLFNKGDIKRAYNYIKIALDDATEYNARHRKIEVGSVLPI
                     IEGERLSTVERQRKQMLNYAFIVSMLSILVISFFAIIFLQLRKINKVKSILQEANNNL
                     REVNAHLIEANKIKEEYIGYFFNVNSEYIEKIESFQKNLHRKIVAKQYNDLDNIINNT
                     DLKKERENLFINFDKIFLKIFPNFVFEFNKLFGPEDQIMPEKGELLNTDLRIFALIRL
                     GITDNDKIAKFLNYSVNTIYTYKTKIKNKTIVDRDLFEQKIMEIKAI"
     gene            1648..2826
                     /locus_tag="PKJ43_02550"
     CDS             1648..2826
                     /locus_tag="PKJ43_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71469.1"
                     /translation="MKRKAILLFALLSLYGIIASAQADFQKGLIILNSNDTVKGLIDL
                     RSSKFNNQICRFRESKKSPIQTYKPNDIKAYILDVGKYYVSKVIDIDGVIDTVFAENI
                     IEGQLSMFFYKDKDFSYYFFEDESKKPIYISNKKVFTYGNDGRKYIKDNITNRGSLVY
                     YLSESQETAQKVNKLTLNQDKLIPIIKEYNEVVCPDKECTVYAKKIKPVFSLGPLLGY
                     NKNTVYIIRSGYVLNEPAISNSMQYGVSGEYTVPALSKGLFVSFSSAFCRNELQYGNK
                     IRPSIIEYNNLNFNASLYYCYQHYRLQPLAGIGIALATTLGSSKRYSTVTPESTTPAK
                     RTMYAPTISAGLKYKINQKIYLKTYLETDFLQTDIRYDKYFLTVSSNINAFFGVFYTL
                     "
     gene            2990..3880
                     /locus_tag="PKJ43_02555"
     CDS             2990..3880
                     /locus_tag="PKJ43_02555"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNZ71470.1"
                     /translation="MKEDKRKVELVVISDIHLGTFGCRAKELLRYLKTIEPDTLVLNG
                     DIIDIWQFKKRYFPKSHMKVIKHFTSLLSKKTKIYYVTGNHDEMLRKFKGFQLGTLEI
                     VNKVVLQLDGKKAWIFHGDVFDNSVNHSKWLAKLGSIGYNFLIVINMLINVVTRLFKG
                     GKISLSKKVKDGVKSAISHINKFEKTIAELAILNGYDFIVCGHTHQPKLQYIADKKGQ
                     EVLYLNSGDWIENLTALEYNNQEWTMYAYANDPVAQMIDEIIPPEPEEIKLDFKIEQF
                     YSELAEEIHVVPMNVLRAKA"
BASE COUNT         1390 a          798 c          756 g         1059 t
ORIGIN      
        1 acttatttca atcaaaaaaa cgagcggata aagaaactga ccacaagtct taaacaaact
       61 gaaaaaaacg ggcaaactac cgcggctttt tccctatgca gcgaacttat cgacgaatac
      121 aagtcgttcg acttcgattc tgcatcgaaa tatgtaacac acttaaattc actggcgtat
      181 aaacttggcg ataaagagaa aattgctcag gcaaaaataa aatcagggtt tacactgctc
      241 tcgtcaggaa tgtttaaaga atcgctcgat tcgttattga gcatcgactc agagaaatta
      301 ccagaaagtt taaagccaga atattacaat gtaatagcca gaacatatta tgacttagct
      361 gactataata gcgatgacta ttattcaagt atttacagaa aacttggaac agcacatctc
      421 gattcagccc tggctttact cgacaagaac agcagtacat actgggcttc gatgggcttg
      481 cgccggatga aagctgacga ttacgaagga gccgccgatg cttttaacta cctgattacc
      541 aaatttgaca ttactgatca cacttacgcc atcgccacat cgagccttgg atacctgtat
      601 acgctgatgg gccgcgataa cgaagctatc gatatgctga ttaaagccgc caatgccgat
      661 attgtttcat cgactaaaga aaccgtggcc ttgcggaacc ttgctgttca tcttttcaat
      721 aaaggcgata ttaaaagggc gtacaactat atcaaaattg ctctcgacga tgccaccgaa
      781 tacaatgcgc gccaccggaa aattgaggtc ggctcggtgt tgcccatcat cgaaggtgaa
      841 aggctctcga cggttgaacg tcagcgcaag caaatgctca attacgcgtt cattgtgtcg
      901 atgctttcca ttttggtaat ctcgtttttt gccattatct tccttcaact aagaaaaata
      961 aacaaggtta agtccatttt acaagaagca aacaacaacc tccgcgaagt gaatgcacac
     1021 cttatcgaag ccaataaaat taaagaagag tatattgggt attttttcaa cgtgaactct
     1081 gaatacatcg aaaaaattga atcgttccag aagaacttac accgaaagat tgtcgcaaaa
     1141 cagtacaacg atcttgataa tatcataaat aacaccgatt taaaaaagga acgagaaaac
     1201 ctcttcatca atttcgacaa aatattccta aagattttcc ccaattttgt atttgaattt
     1261 aacaaactct tcgggccaga agaccaaata atgccagaaa aaggagaatt gctgaatact
     1321 gatttacgca tatttgccct gataaggctt ggcataaccg acaacgataa aattgccaag
     1381 ttcctgaact attcggtaaa tacgatctac acatataaaa caaaaataaa gaacaaaacc
     1441 attgtcgatc gcgacctttt cgagcagaag ataatggaaa tcaaagcaat ataagagcag
     1501 actcagacgg ggtaattata aacatagagc atttatttaa tccagaattt taaaaaaatc
     1561 tttttgaaga aaaagaaagc atttaatgaa atgtttatac ttttatccat caaaaaaatg
     1621 ggaataccct ttaagaaaga aaacattatg aaaagaaaag ctatcctact ttttgctctt
     1681 ttatcactct atggaataat tgcttcggct caggctgatt ttcaaaaagg gcttataatt
     1741 ctcaacagca acgacaccgt taaagggtta atagacttaa gaagctcaaa attcaacaat
     1801 caaatttgcc ggtttaggga aagtaaaaaa agtccaatac aaacctataa acccaacgat
     1861 attaaagcgt atatcttaga cgttggaaaa tattatgtat ctaaagttat cgacatcgat
     1921 ggagtaattg atactgtttt tgcagaaaac atcattgagg gacagttaag tatgtttttc
     1981 tataaggata aagatttttc gtattacttt ttcgaagatg aaagcaaaaa gccaatatac
     2041 atatcgaaca aaaaggtttt cacctatgga aatgatggca gaaaatatat caaggataat
     2101 ataaccaaca gaggatcatt ggtatactat ctaagcgaaa gccaagaaac agctcaaaaa
     2161 gttaataaat taacgctaaa ccaagataaa ctaataccca ttattaagga atacaacgag
     2221 gtagtttgcc ccgataagga gtgtaccgtt tatgcaaaaa aaataaaacc ggtttttagt
     2281 ctgggcccat tattgggata caacaagaat acagtgtata taattcgtag tgggtatgtt
     2341 ttgaatgagc cggctatctc caatagtatg caatatggtg tttctggtga atatacagtc
     2401 cccgcattaa gcaaagggct tttcgtttcg ttttcttcgg cgttttgcag gaacgaactc
     2461 caatatggaa acaaaatccg ccccagcata attgagtaca acaacctgaa tttcaatgct
     2521 tcgctatact attgctacca acattacaga ttgcagcccc ttgcaggaat tggaattgct
     2581 ttagcaacca cactgggttc gtcaaaacga tacagtactg tcactcctga aagtactacc
     2641 ccagccaaaa gaaccatgta cgcaccaacc atttcagctg ggcttaaata caagattaat
     2701 cagaaaatat acttaaaaac atatcttgaa accgatttcc ttcaaacaga tataagatat
     2761 gataaatatt ttcttaccgt gtcttcaaat attaacgcct tctttggcgt cttctatacc
     2821 ttgtaaaata tggcatagca cagcccctgc taaataatac aaaagcttat acgagccaac
     2881 ctttgttgaa ataaagatta gaaatagctc ataggacaaa cttgtattta atcatctttt
     2941 aatcagaatt aaaacgacct gtaacatttc tgcattttct ttacgatcaa tgaaagaaga
     3001 taagcgaaaa gttgagctgg ttgtcatatc cgacatacac cttggcactt tcgggtgtcg
     3061 tgcaaaagag cttctgaggt atttaaaaac gatcgaaccc gatacgttgg ttctcaacgg
     3121 cgatattatc gacatctggc aatttaagaa acgctacttt ccaaagtcgc acatgaaagt
     3181 aatcaagcac tttacctccc ttttatcgaa aaaaacaaaa atctactatg tgaccggcaa
     3241 ccacgacgag atgctgcgaa agttcaaagg gtttcaactt ggcacgctcg aaatcgtgaa
     3301 caaggtagtt ttgcaacttg atgggaaaaa agcatggata tttcatggcg acgtgttcga
     3361 caactcggta aaccactcga aatggcttgc aaagctgggc agcatcggat acaatttcct
     3421 gatcgtgatc aatatgctga tcaatgtagt aacccggttg tttaaagggg gaaagatttc
     3481 gctttcgaag aaggtgaaag acggggtgaa aagtgccatt agccatatca ataaatttga
     3541 aaagaccata gctgagctgg ctattctgaa cgggtacgat tttattgttt gcggccacac
     3601 ccaccaaccc aagcttcaat atatagccga taaaaagggg caggaagtgt tgtacctaaa
     3661 ctcgggcgat tggatcgaga acctcaccgc ccttgaatac aacaaccagg aatggaccat
     3721 gtacgcctat gcaaacgacc ctgttgccca aatgatcgat gaaataattc cgcccgaacc
     3781 ggaagagatt aagctcgatt tcaagattga acagttctac tccgaattgg ccgaggagat
     3841 tcacgttgtc ccaatgaatg tgttaagggc aaaagcatga gaattcttta tgccattcag
     3901 ggaacaggca acgggcatct tagccgtgcc accgatatcg ttccggctct tcgacaatac
     3961 ggggatgtgg atattctggt aagtggaaag aattcacagg ttg
//
LOCUS       DAOPUW010000096         3999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_22732_length_3999_cov_4.068205, whole genome shotgun sequence.
ACCESSION   DAOPUW010000096 DAOPUW010000000
VERSION     DAOPUW010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22732_length_3999_cov_4.068205"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="PKJ43_02560"
     CDS             <1..1432
                     /locus_tag="PKJ43_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ71471.1"
                     /translation="CDVGLGYLTLNRLSNTLSGGESQRINLATSLGSGLIGSLYILDE
                     PSIGLHPRDTERLIGVLKKLRDLGNTVIVVEHDEDIMKAADMLIDIGPYAGTNGGEIM
                     FEGNIEELSKDTKSLTALYINGIEKIEVPAQRRKWNNFFQVTGARENNLKNIDVKIPL
                     NVMTVITGVSGSGKSSLVKQIIYPAIAKRFGAYDEKTGKHDKLTDDVHLLHQVEFVDQ
                     NPIGKSTRSNPVTYLKAYDEIRSLFADQQSAKILGLKPAHFSFNVEGGRCDECQGEGV
                     INVEMQFMADISLVCESCGGKRFKEDILDIHYHEKNIYDVLEMTVDEAIDFFAHGKSN
                     TEKKIVKKLKPLQEVGLGYIKLGQSSSTLSGGESQRVKLASFLAKEQDSPTLFIFDEP
                     TTGLHFHDIKTLLKAFNELLSRNHTLLIVEHNQEIIKSADWVIDLGPEGGENGGNIVF
                     AGTPEEVCSCKESYTGKFLKSKIANA"
     gene            1474..1992
                     /locus_tag="PKJ43_02565"
     CDS             1474..1992
                     /locus_tag="PKJ43_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71472.1"
                     /translation="MATDKISKKDQQYITDLQSGNFGKITSALTQLEEDGSIVVLPAI
                     IELLNSTVDDDITKRAYKLLCELKQTASVPIIVEAIMNKEYSSIQEMLLRACWENGLD
                     YSKYLPTFVNIVIHGSFMNAFEAFTVIENMEQAIDENIANEMASILSNALNSVTTEKE
                     SLIKDLIQQFNP"
     gene            complement(2019..2735)
                     /locus_tag="PKJ43_02570"
     CDS             complement(2019..2735)
                     /locus_tag="PKJ43_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNZ71473.1"
                     /translation="MNCIIIDDDKLSRTLLEKFVERTEYIDQYYSFPGAIEAINFIKK
                     DEHEIDLIFLDIEMPDMNGVEFMQALGDHPVQIIVVSSKEKYALDAIEYNVTDYLLKP
                     VTYFRFLKATEKALSKLREEMMPASNSKNDFFIRNNANLIRLCYDDVIWVEALENYIV
                     VNTFGEKFTIHYTMKMVMDQLPVDKFFRIHRSFIVNRSKIDIIKATTIDVKTKDGYVS
                     LPIGKSYRELLLKDINLLSK"
     gene            complement(2801..3475)
                     /locus_tag="PKJ43_02575"
     CDS             complement(2801..3475)
                     /locus_tag="PKJ43_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNZ71474.1"
                     /translation="MSKELVDYLSSFLLDSRLELFKKNLSLRTRYITVVLEDIFQSQN
                     ASAVIRSCDCFGIQDLHIIENDNKFSEYERVTRGSSQWVDFHYYNQQQNNTLDAIQKL
                     KQQGYRIVATSPHDSDIDLHHFDLLQGKAAFIFGNEKRGISDVVKQNADCFMKIPMYG
                     FTESLNISVSVAIVAHYLSAQLRLLPVSWQLTDEESTAVMLDWLKKTLKRSDLLVKKF
                     HTQKVS"
     gene            complement(3553..3852)
                     /locus_tag="PKJ43_02580"
     CDS             complement(3553..3852)
                     /locus_tag="PKJ43_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNZ71475.1"
                     /translation="MYFITLKSQLMNKAQLIDAIAEKAGLTKADSKKALDAFIEATSE
                     ALKAGDRVALVGFGSFSVAKRSARTGRNPQTGKEITIPAKAVVKFKPGSELSEGV"
BASE COUNT         1330 a          775 c          746 g         1148 t
ORIGIN      
        1 ttgcgatgtt ggattgggct acctcacact aaaccggttg tcgaacaccc tttcgggagg
       61 agaatcgcag cgtataaacc ttgccacttc gctcggcagc gggctaattg gctctttgta
      121 tatcctggac gaacccagta taggacttca ccccagagat actgaaagac taattggagt
      181 attgaaaaaa cttcgtgact tgggcaatac agtaatcgtt gtcgagcacg acgaagatat
      241 tatgaaggct gctgatatgc tgatcgacat tgggccttat gccggcacca acggaggtga
      301 aattatgttt gaaggcaaca tcgaagagct tagcaaagat accaaaagcc ttacagccct
      361 gtacatcaac ggaattgaga agattgaagt cccggcgcaa cgaagaaaat ggaacaactt
      421 ttttcaagtt acaggagcaa gggaaaacaa cctcaagaat attgatgtaa aaattccttt
      481 aaacgtgatg actgtaatca ccggagttag cggatcggga aaatcgtcgt tggtaaaaca
      541 aattatctac ccagcaattg ccaaacggtt tggggcatac gacgaaaaaa cgggaaaaca
      601 tgacaagcta actgacgatg tgcacttact tcatcaggtt gagtttgtcg atcaaaaccc
      661 gattgggaaa tcaacccgat caaacccggt tacatatctt aaagcctacg acgaaatacg
      721 ttctcttttt gctgaccagc aaagtgctaa aatactagga cttaaacctg ctcatttttc
      781 gttcaatgta gaaggcggcc gctgcgacga atgtcagggc gaaggggtta tcaatgtaga
      841 aatgcagttt atggccgaca tttcgctggt ttgcgaaagc tgtggaggaa aaaggtttaa
      901 agaagacatt cttgacattc actatcacga aaagaacatt tacgatgtac ttgaaatgac
      961 cgttgacgaa gctattgact tctttgccca tggaaaaagc aatacagaaa agaagattgt
     1021 aaaaaaatta aaacctcttc aagaggtagg attaggatac attaaactcg gacaatcgtc
     1081 gagtacactc tccggagggg aaagtcaacg ggtaaaattg gcctcgtttc ttgcaaaaga
     1141 acaagactct cctacccttt tcatcttcga tgagccaaca accggcctgc actttcacga
     1201 tataaaaaca ttactaaaag cgtttaacga attacttagc cgaaaccaca ctttgttgat
     1261 cgttgaacac aaccaggaga taataaaatc agccgattgg gtaattgatt taggccccga
     1321 aggcggtgaa aatggaggaa atattgtgtt tgccggcacc cctgaagagg tttgctcttg
     1381 caaagaatcg tataccggga aattcttaaa aagtaaaatc gccaacgcct aatgcacttt
     1441 tcattttcac aacaaacaaa ataacaacaa gatatggcaa cagacaaaat ttcaaaaaaa
     1501 gaccaacaat acatcacaga cctccaatca ggcaacttcg gtaaaataac cagtgcatta
     1561 acgcagttgg aagaagacgg gagcattgtt gtacttccgg caattataga gctactaaac
     1621 tcaacggttg acgacgatat taccaagaga gcctataaac ttctttgcga actgaagcaa
     1681 accgcatcgg tgccaattat tgtcgaagcc attatgaata aagagtattc ctcaattcag
     1741 gaaatgcttt tgcgtgcatg ctgggaaaac gggctcgatt acagcaaata ccttcctact
     1801 tttgtcaaca ttgttattca tggaagtttt atgaatgcat ttgaagcttt tacagtaatc
     1861 gaaaatatgg aacaagctat cgatgagaat attgcaaacg aaatggcaag tattttatct
     1921 aatgctctga atagtgttac gacagaaaaa gaatcgctga ttaaggattt aatccaacag
     1981 tttaacccat aaaaaaacct gcttcacgca ggttttttct atttcgacaa tagatttata
     2041 tcttttaaaa gcagttccct gtatgactta ccaataggga gagatacata accatctttt
     2101 gtttttacat caattgtggt tgctttaatt atatcaattt tactgcggtt tacaataaaa
     2161 gaacggtgaa tacggaagaa tttatcgaca ggtaattggt ccataaccat tttcatggtg
     2221 taatgaattg taaatttctc gccgaatgta ttcacaacaa tataattttc aagggcttca
     2281 acccaaatta catcatcata gcaaagcctt ataaggtttg cattatttct tataaaaaag
     2341 tcatttttag agtttgaggc aggcatcatt tcctcgcgaa gcttcgaaag cgctttttct
     2401 gtagccttca aaaacctgaa gtaagtcaca ggtttgagca aataatcggt cacattatac
     2461 tcaattgcat cgagagcata cttctctttc gaagaaacaa caataatttg gacaggatga
     2521 tcgcccaatg cctgcataaa ttcaacgcca ttcatatctg gcatttcaat gtcgagaaat
     2581 atcaaatcaa tttcgtgttc atcctttttg atgaaattaa ttgcctcaat agcaccgggg
     2641 aaagagtaat attgatcaat atattctgtc cgttctacaa acttttcaag caatgtcctt
     2701 gataacttat catcatcaat aattatacaa ttcatagttt tattatcaag ttagattttg
     2761 ataaggctaa attaaaattt taataaaagt aaactaaaaa ttaagatact ttttgtgtat
     2821 gaaatttttt cactaataaa tcgcttcttt ttaaagtttt cttcaaccag tcgagcataa
     2881 cagccgtcga ttcttcatca gtcaactgcc aactaacagg caaaagccgc aattgtgccg
     2941 acaaataatg ggcaacaata gccaccgaca ccgaaatatt aaggctttcg gtaaaaccgt
     3001 acatgggtat tttcatgaaa caatcggcat tttgttttac aacatctgat attccacgtt
     3061 tctcgttacc aaagatgaaa gctgcttttc cctgtaagag atcgaaatga tgcaaatcga
     3121 tatcggaatc gtgaggcgat gtggctacaa tacgataacc ttgttgtttt aacttttgta
     3181 ttgcatcaag tgtgttgttt tgctgctggt tataataatg aaaatcgacc cattgagatg
     3241 accctcgggt aactctttcg tattcactga atttattatc attctcgatg atatgtaaat
     3301 cttgaatgcc aaaacaatcg caagaacgaa taacagcact cgcattctga ctttgaaaaa
     3361 tatcctccag aacaacggtt atataacgtg ttcgcaatga aaggtttttt ttaaataact
     3421 caagacggct gtctaataga aaagaagaaa gataatcgac taactctttc gacatagaaa
     3481 cagattgatt gggtaaataa aaaaagggtg cttcaggaat tgagacaccc ttcacaacat
     3541 cttttgaaag tattatacac cttcagataa ctcgcttcct ggtttgaatt ttacgacagc
     3601 ttttgcgggg atggtaattt ctttgcctgt ttgagggtta cgaccagttc ttgcacttct
     3661 ttttgctaca gaaaaagaac caaaaccaac taatgcaaca cggtcaccag cttttaaagc
     3721 ttcgcttgta gcttcaataa aagcatcgag agcttttttt gagtcagctt ttgttaagcc
     3781 tgctttttca gcaatggcat caattaattg agctttattc ataagttgag atttaagggt
     3841 tataaaatac attaactccc acaaatttat tgtttttgac gagttttcca aaccttaaca
     3901 ataaaaaaaa tactgccttt tccaacaaat tagcgggctt cagatcgttt tttttcaatt
     3961 ttgaagcaca attaaaaccg ccaaaacagc ctaagtcct
//
LOCUS       DAOPUW010000097         3991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_22796_length_3991_cov_3.853404, whole genome shotgun sequence.
ACCESSION   DAOPUW010000097 DAOPUW010000000
VERSION     DAOPUW010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22796_length_3991_cov_3.853404"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PKJ43_02585"
     CDS             complement(<1..287)
                     /locus_tag="PKJ43_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71476.1"
                     /translation="MVRENRNPLSSDRNILSIILVGIVLILIAFWPTFNAGFVNWDDN
                     LYVYENATVQSLSNLGKIISEPVAGNYHPLTMISLAVDYAISSGKAGWFHAV"
     gene            551..2362
                     /locus_tag="PKJ43_02590"
     CDS             551..2362
                     /locus_tag="PKJ43_02590"
                     /inference="COORDINATES: protein motif:HMM:NF039640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6057 family protein"
                     /protein_id="HNZ71477.1"
                     /translation="MKKIRPLIWSLAVILLGIISAIFLYKFINPLLINFKQQPLFLTG
                     SPFFSSFMEYPGGLTEYLSLFFSQFFVSRVVGTLVILAFATANAVVLYYLAKKHLGQA
                     ISLGLAISVGILLTGLHASYEATLQTSIVLLISSLSVFFLQLLNTRKNYFKIPLIVFL
                     GSLMYFYIGATALMAFTALFAATTLYKKDVSNYLTALLLIGACFALPAYTSATSAYMG
                     YKEASLGILEPTRKPFFFVQLITFLISPVLILLSPLKKKMSIKPLAINTIHIALPILL
                     IAFSTAFYYFSFNKDKKNNLLFHYLAKNKQWAEVISIGKELSFNDRKVPFQLNRAYYH
                     QNILIHKAFQIPQYWGERGLILTSHYNSEVLMLCSDLYYDMGHIKASLHWAYEAQTKF
                     PFSPDVLQRIILCNLILNEFNTAEHFIDMLSRSVIYRKWAEQQRKFLNKPQLVSSDSE
                     LGEKQKLTPDEDFFTNQIQPIYDLSMLLKKNPSNKMAFEYYMIYAMLTHDLVKMEKEI
                     HCMGSVGYKKIPIHLEEAILLFSVLNKEKKIDTGYYTVSQETSQRFRAYSELMMKYRS
                     NLKQGEPEFRQGYKDTYWYYIHFVSPITTKREFKEKK"
     gene            2368..3897
                     /locus_tag="PKJ43_02595"
     CDS             2368..3897
                     /locus_tag="PKJ43_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71478.1"
                     /translation="MKRKVYIALAIAMCIVAVFFLLPAKEPGRSTPAKQEAPIFPDYS
                     SVTLPYNIAPINFMVEDGAEKVIATFSSKSGKKIVVKSSNNKIEIPIQAWKKLLSANK
                     GNDLIITIYSKKKGSAWVKYPSITNYISSDEIDSHIVFRHISAGYILWEKMGIYQQNI
                     ETSEKKPILLNDRTNRNCMNCHTFGNYDPQKMLIHLRSKPGGTILYNNSEAKLLNTKT
                     PFTMSSCVYPAWHPNGKAIAFSVNIIEQKFHSAGNEAINVYDKSSDLVVYDVEKNMIT
                     TTPAISTSSMENLPSWSPDGKYLYYISAPAQRGQNDSLKTRYDLLRIPYSYEQNTWGL
                     ADTLLRSSDTGLSITFPEVSPDGRFIVFCMADQGYFSVHYTSSDLYIMDIATREYSKL
                     PINSDHVESFHYWSSNSKWLMFISKRLDNLYSRVYFTHIDEKGNASKPFLMPQTDPET
                     NRTLNLNYNRPVFVNGEVTISPASLAEVANESPVDVKFDPSVNIDALSGATRYAGKTV
                     H"
BASE COUNT         1286 a          905 c          693 g         1107 t
ORIGIN      
        1 accgcgtgaa accacccagc ttttccgctc gatatggcgt aatctacagc aagcgagatc
       61 atagtcagtg ggtgataatt gcctgcaaca ggttcactaa taatctttcc taaatttgaa
      121 agcgattgaa cagttgcgtt ctcatacaca tacaaattat catcccagtt tacaaagccg
      181 gcattgaatg ttggccagaa ggcaatcaat atcaacacaa taccaaccag gattatcgac
      241 agtatatttc tatcggagga taatgggttt ctgttctctc taaccatttc gacgaaagtt
      301 atttagctta ccaaacaata ggtagctgca aatttgtaga tacgaactta attatcaaaa
      361 ttattacagc tttttctcaa gcatttcact ccctgaatca tcatgttttc gatgtcctta
      421 accctctgaa tatctatgag cagcacctat tttttcctct caataaaata aaatagtcat
      481 tgataaacag attatagtct aaatactata tttgtgctta tcaaaaaaac cgttgaatgc
      541 ttctctgaaa atgaaaaaaa tacgtccgtt aatctggtcg cttgcagtaa tacttctggg
      601 tatcataagt gccattttcc tatataaatt cattaatcct cttttaataa actttaaaca
      661 acagccactt tttctcactg ggagtccttt tttttcgtca ttcatggaat atccaggtgg
      721 tctaaccgag tacttgtcgc ttttttttag tcaatttttt gtttcacggg ttgtcggtac
      781 tttggtaatt cttgcatttg ccacagcgaa tgctgttgtg ttgtattatc ttgccaaaaa
      841 acacctcggg caagccatct ccctgggact ggctataagt gttggcattc tgcttaccgg
      901 actccatgcc agctacgaag caaccctgca aaccagtatc gttctgctca tttcctcttt
      961 gtcagttttt tttcttcaac tacttaatac aagaaaaaac tatttcaaaa tacccttaat
     1021 tgtctttctg ggaagcctca tgtatttcta tattggtgcc acagctctca tggctttcac
     1081 cgcattattt gcagccacca cactgtacaa aaaagatgta tcaaattatc tgacggcact
     1141 actgttgatt ggcgcttgtt ttgcgcttcc tgcttatacg tcggcaacat cggcgtatat
     1201 gggatacaaa gaggcctcat tgggtattct tgaacccacc cgtaagccat ttttcttcgt
     1261 tcaactcatt acctttttaa tcagcccggt gctaattctg ctttccccat taaagaaaaa
     1321 aatgtcgata aaaccactgg caataaacac aatacacatt gccttgccaa tattactaat
     1381 agccttttct acagcatttt attatttttc attcaataag gataaaaaga ataaccttct
     1441 ttttcattat ctggccaaga acaaacagtg ggccgaagta atttccattg gaaaagagct
     1501 atcatttaac gaccgaaaag tacctttcca gctcaaccgc gcatactacc atcaaaacat
     1561 actgattcac aaagcttttc aaatacccca atattggggc gaacggggct taattctcac
     1621 gtcacattat aatagcgaag ttttgatgct atgcagcgac ttgtattacg acatggggca
     1681 cattaaagcc tccttacatt gggcctacga ggcacaaacc aaattcccgt tttctcccga
     1741 tgttcttcag cgaattattc tctgcaacct tattttaaac gaattcaaca ctgccgaaca
     1801 ctttatcgat atgctgtcac gatcagtcat atacagaaaa tgggctgaac agcagcgaaa
     1861 atttcttaat aagccacagc tcgtttctag cgactctgag cttggtgaaa aacaaaaact
     1921 cacacccgat gaagattttt tcaccaacca aatacaaccc atctacgacc tgtcgatgct
     1981 cttaaagaaa aatccaagca acaaaatggc cttcgaatac tatatgatat atgccatgct
     2041 cactcacgat ttggtaaaaa tggagaaaga aatccactgc atgggaagtg ttggatataa
     2101 aaagattccc atccatctgg aagaagccat cctgcttttt tcggtgctga acaaagagaa
     2161 aaaaatcgac acaggatatt ataccgtaag tcaggaaaca tcccaacgtt tcagggcata
     2221 ttcagaattg atgatgaagt accgtagcaa cctgaaacaa ggagagccag agttcagaca
     2281 agggtataaa gacacctact ggtattatat tcattttgtt agccctataa caacaaaacg
     2341 tgaattcaaa gaaaaaaaat aataccaatg aaacgtaagg tttatatagc tttagccatt
     2401 gccatgtgca tagtcgcagt atttttttta ctgccagcca aagaacccgg gagaagcacg
     2461 ccagcaaaac aagaggcgcc cattttcccc gattattctt cagttacgct gccttataat
     2521 attgccccta taaattttat ggtcgaagat ggtgcagaaa aagttatagc cacattcagc
     2581 agtaaatcag gaaaaaagat tgttgtaaag tcatcaaaca acaaaattga aatacctatt
     2641 caggcatgga aaaaactgct tagtgcaaat aaaggtaacg acttaatcat tactatttat
     2701 tcgaagaaaa aaggaagcgc atgggtgaaa tatccttcca taacaaatta tatttcaagt
     2761 gacgaaatcg acagccacat cgttttcaga cacataagtg ccggatatat cctctgggaa
     2821 aaaatgggca tttaccagca aaatattgaa acatcagaaa agaagccgat attactcaac
     2881 gaccgcacca accgcaactg catgaattgc cacaccttcg ggaattatga ccctcagaaa
     2941 atgctcatcc atttgcgaag taagccgggc ggaaccatac tatataataa tagtgaagca
     3001 aaattactga acacaaagac ccctttcacc atgtcgtcgt gcgtatatcc tgcatggcat
     3061 cccaatggta aagccattgc tttttcggtg aacattatcg agcagaaatt tcacagtgcc
     3121 ggcaacgaag ccatcaatgt atacgataaa agttccgatt tggttgtgta cgatgtcgaa
     3181 aaaaacatga taaccacaac ccccgcaatt tcaaccagca gcatggagaa ccttccaagt
     3241 tggtcgcccg acggaaaata tctatattac atttctgcac cagcacaaag aggacaaaac
     3301 gacagtctga aaacccgcta cgatttgctc cggatcccct attcatatga acaaaataca
     3361 tggggcctcg ccgacaccct gttgcgctca tctgatacag gcttgagcat taccttccct
     3421 gaggtatcgc cagacgggcg ctttatagtt ttttgcatgg ccgaccaagg atacttttca
     3481 gtacattaca cctcgagcga cctttacatt atggatattg ctacacgcga atattcaaaa
     3541 cttcctataa acagcgacca cgtagaaagt tttcattact ggtcatcgaa cagcaaatgg
     3601 ctcatgttca tcagtaaacg gctcgacaac ctgtattcga gggtctattt cacccatatc
     3661 gatgaaaaag gcaatgcctc aaagcctttt ctcatgccac aaacagaccc cgaaacaaac
     3721 cgaacgctta acctaaacta caacagaccg gtatttgtaa atggagaagt tacaataagc
     3781 cctgcttccc tggcagaagt agccaatgaa agtcctgtgg atgtaaaatt tgacccctct
     3841 gtgaatatcg atgccttgtc gggagccacc cggtatgctg gaaaaacagt gcattaacag
     3901 ctaccgtcag aacaagaaaa taaccattgg atataaaaac atcagtttgt aagaaatgaa
     3961 cagttttttt gaaacgcaaa aacaaccacc t
//
LOCUS       DAOPUW010000098         3981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_22882_length_3981_cov_3.832654, whole genome shotgun sequence.
ACCESSION   DAOPUW010000098 DAOPUW010000000
VERSION     DAOPUW010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22882_length_3981_cov_3.832654"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1311)
                     /gene="clpX"
                     /locus_tag="PKJ43_02600"
     CDS             complement(97..1311)
                     /gene="clpX"
                     /locus_tag="PKJ43_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNZ71479.1"
                     /translation="MDKCSFCGRTKDEVELLIAGMDGHICDRCIEQAYAIVEEEFKGK
                     GEFDISGIKLSKPKEMKEFLDQYVIGQDHAKKILSVAVYNHYNRLAQKIDQDETELEK
                     SNIILVGPTGTGKTLLARTIAKMLHVPFTMVDATVLTEAGYVGEDIESLLTRLLQAAD
                     YNVEAAEKGIVFIDEIDKIARKGDNPSITRDVSGEGVQQGLLKLLEGSIVNVPPQGGR
                     KHPEQRMIPVNTKNILFICGGAFDGIERKIAQRLNTKVVGFNAIEGTATINRDNLMQY
                     ITPMDLKSFGMIPEIIGRLPVITYLDPLDKDALRRILTEPKNAIVKQYIKLFELDGIA
                     LNFNDSALEYIVNIAVEFKLGARGLRSICETIMVDAMFEMPSNGKKKFTIDEKYAREK
                     IESSGMQLLKAG"
     gene            complement(1320..2000)
                     /gene="clpP"
                     /locus_tag="PKJ43_02605"
     CDS             complement(1320..2000)
                     /gene="clpP"
                     /locus_tag="PKJ43_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNZ71480.1"
                     /translation="MDTRKEFQKYAKSAAGISSLSSDKYINSVYGSYISPTIIEERQL
                     NIATMDVFSRLMMDRIIFLGTAIDDYVANIIQAQLLFLDSTDPGKDIQIYFNSPGGAV
                     YAGLGIYDTMQFISSPVATICTGMAASMSAVLLTAGATGKRSALTHSRIMIHQPMGGT
                     QGQAVDMEITVREIVKLKKELYEIIAKHSGNDIEKIEKDSDRDYWMTAQEAKDYGMID
                     QVLVRNKK"
     gene            complement(2172..3530)
                     /gene="tig"
                     /locus_tag="PKJ43_02610"
     CDS             complement(2172..3530)
                     /gene="tig"
                     /locus_tag="PKJ43_02610"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNZ71481.1"
                     /translation="MNITKENIDSLSGIIRVNIEEADYQANVEKALSDYRKKANMPGF
                     RPGKVPQGLVKKMYGKAALADEVNKLLSDGLSKFIVDEKLDILGEPLPHEELQQTIDW
                     DNDTNFEFVFEIGYAPEVKISLDKRTKLPYYKVNVSNELIDRQVESYAARFGENKPVD
                     AIEKDSTVRGDIVQLDENNAVAEGGHSQEMGLISVDLIKDEEIKNSFVGKNAGDEIIF
                     DIKKAYPNDTEIAYLLNIEKKDAENVSGNFKFTVKEINKFVAAELTEEHYKKILGEET
                     QILTAEAFREHVANELSVYYKPSADYKLAIDARELLINKIKMEFPEGFLKRWLLATNK
                     ELTQEQIDADFEPFMEDLRWQVIKENIIKENELKVSQEDVMELAKDIAASQFRQYGMF
                     DIPEEHLEGFARQMLEKKDERNRLYSKKMEDKVIEVIKSKVGIEEKEVTNEEFEKLFE
                     KK"
     gene            complement(3637..3718)
                     /locus_tag="PKJ43_02615"
     tRNA            complement(3637..3718)
                     /locus_tag="PKJ43_02615"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3682..3684),aa:Leu,seq:tag)
     gene            complement(3784..>3981)
                     /locus_tag="PKJ43_02620"
     CDS             complement(3784..>3981)
                     /locus_tag="PKJ43_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNZ71482.1"
                     /translation="GILITDHNVHETLAITDRSYLLFEGSILKAGTAEELAADEMVRK
                     VYLGQNFELRKKREKFIHASG"
BASE COUNT          953 a          849 c          832 g         1347 t
ORIGIN      
        1 aatcttactg tttacaagcg gtctgaactc aaaaataaag tcgaactcag gttataagga
       61 aaaaaaatcc cgcacctggc gggattcaat atcttattaa cctgctttca aaagctgcat
      121 tcccgaactt tcgatttttt cccgtgcata cttttcatca atagtaaatt tctttttacc
      181 gttcgaaggc atttcgaaca tggcatctac catgatggtt tcacaaattg aacgtaaccc
      241 acgggccccc aatttgaatt caacagcaat attcacgatg tattctaggg ccgaatcatt
      301 gaagtttaat gcaattccgt cgagttcaaa cagcttgata tattgcttta caatggcatt
      361 ctttggttcg gtaagaatac gcctcaacgc atccttgtcg agtgggtcga ggtaagtgat
      421 tacaggtaag cgtccaatga tttcaggaat catcccgaac gactttaggt ccatcggggt
      481 gatatattgc atcaggttgt cgcggttaat ggtggctgtt ccttcaatgg cattgaagcc
      541 aacaaccttg gtattaagcc gctgagcaat tttgcgttct atcccgtcga aagctcctcc
      601 gcaaatgaat aatatgtttt tcgtatttac cgggatcatt cgttgttcgg ggtgttttcg
      661 tccgccttgt ggaggtacgt taacaatcga cccttcgagc aatttcagca agccttgctg
      721 aactccttcg cccgacacgt cgcgggtaat ggatgggtta tcgcctttgc gggcaatttt
      781 atcaatttca tcaatgaata cgatgccttt ttctgctgct tctacgttat agtcggctgc
      841 ctgcaataag cgggttagaa ggctttcaat gtcttcacct acgtaaccgg cttcggtgag
      901 cacagtagca tcaaccatgg taaaaggcac gtgtagcatt ttggcaatgg tgcgtgccag
      961 cagcgtttta ccggtacctg ttgggccaac aaggatgatg ttcgattttt ctaattcggt
     1021 ttcgtcctga tctattttct gggcaagccg attgtagtga ttatacacgg ctaccgataa
     1081 tatctttttc gcatggtctt gaccaataac gtactggtcg agaaattctt tcatctcctt
     1141 cggtttcgac agcttgattc cacttatgtc gaattctcct ttccccttga attcttcttc
     1201 aacgatggca taagcttgct caatgcaacg gtcgcaaata tgaccatcca tgccggcaat
     1261 cagaagctca acttcatctt ttgttcttcc gcaaaacgaa catttatcca ttcaatatat
     1321 tatttcttgt ttctaactag tacttggtca atcattccgt aatctttggc ttcctgggca
     1381 gtcatccagt aatcgcggtc tgagtctttt tctatttttt cgatatcgtt gccagagtgt
     1441 ttggcaataa tttcgtaaag ttcttttttg agttttacaa tttcgcgaac ggttatttcc
     1501 atgtcaactg cctgtccttg agtgcctccc atgggttgat ggatcatgat ccgtgagtgg
     1561 gtaagtgccg agcgtttgcc tgttgctcct gctgtgagca gcactgctga catcgaggcg
     1621 gccatgcctg tgcatatagt agctaccggc gaagaaatga attgcatcgt gtcgtaaatt
     1681 cccaacccgg catacactgc tccaccaggt gagttaaagt atatttgtat gtctttccct
     1741 ggatcggtag aatcgaggaa gagcaattgt gcctgaataa tattggccac ataatcgtcg
     1801 atggctgtgc caaggaagat gatacggtcc atcatcaacc gtgaaaatac atccatggtg
     1861 gcaatattta gctggcgttc ttcgatgatc gttggagaaa tgtagcttcc atataccgaa
     1921 ttgatgtatt tgtctgatga taagctactt attcccgctg cgctttttgc atatttttgg
     1981 aattcttttc tggtatccat tgtgtcttct tttttgtttt tgaaaaaatt tgcttttgaa
     2041 atgttttagc aagtaaggta acaaagttaa taaaaaaggg ttattatttt tagcagttat
     2101 ttttccgtcg attttggaat cttccttttt cgaaaaaaaa agccggttga aaaagaaccg
     2161 gctcatttgt tttatttttt ctcgaacaac ttttcaaatt cttcgttggt gacctctttt
     2221 tcttcgatgc cgacttttga tttgattacc tcaattactt tgtcttccat ttttttgctg
     2281 taaaggcggt tgcgttcgtc tttcttttca agcatttggc gggcaaaacc ttcgaggtgc
     2341 tcttcaggga tatcgaacat gccgtattga cggaattgtg aggctgcaat atctttggca
     2401 agctccatta cgtcttcctg gcttactttg agttcattct ctttaatgat gttctcttta
     2461 attacttgcc agcgaaggtc ttccatgaac ggttcgaagt ctgcatcaat ttgttcctgg
     2521 gtgagttcct tgtttgtggc aagcaaccag cgtttcagga atccttcggg gaattccatc
     2581 tttattttgt tgatgagcaa ttcgcgtgcg tcgatagcca atttataatc ggccgatggt
     2641 ttgtaataaa ccgacagctc gttggctaca tgttcgcgga aagcttctgc tgtaagtatt
     2701 tgggtttctt cacccaatat ttttttgtag tgttcttcgg tgagttcggc agcaacaaat
     2761 ttgttgattt ctttcaccgt aaatttgaag tttccgctta cgttttcggc atctttcttt
     2821 tcaatgttca atagataagc tatttcggtg tcgttagggt acgctttttt gatatcgaaa
     2881 ataatctcgt cgccggcgtt tttaccaacg aagctgtttt ttatttcttc atctttaatc
     2941 aggtcgactg atataaggcc catttcttgt gaatgaccac cttcagctac tgcattattt
     3001 tcgtcgagct gaacgatatc gccgcgaaca gttgagtctt tttcaatggc atcaactggt
     3061 ttgttttcgc caaaacgtgc agcatacgat tctacctgac ggtcgatcag ctcgttcgat
     3121 acatttactt tataataagg caattttgtt cttttgtcga ggctaatttt tacttcggga
     3181 gcatagccaa tttcaaagac gaattcaaag tttgtgtcgt tgtcccagtc gattgtttgc
     3241 tggagttctt catgtggaag gggttctccc aggatgtcga gtttttcgtc gacaataaat
     3301 tttgacagcc catcggagag aagtttgttt acttcatcgg ccaaggcagc ttttccgtac
     3361 atttttttca caagaccctg tggaactttt ccggggcgaa aacctggcat gtttgctttt
     3421 ttacgataat cgctcaaggc tttttcaaca tttgcctgat aatcggcttc ttcaatgttt
     3481 accctgataa tcccgctcaa cgaatcgata ttttcttttg taatattcat attactgaaa
     3541 agttaatttt tatgacaaga acaacaaaaa acgtgtttca ataaacttaa aaccgcctgg
     3601 aacgtttttc ggggctccaa acggttaagg tttttgtgcg ggcgaaggga ctcgaaccct
     3661 tacgccttac ggcaccagat cctaagtctg gctcggctac caattacgac acgcccgcaa
     3721 gaaagcggcg caaatataaa tcttttttta tttctgcaca atcggagggt agtttattgc
     3781 atgtcaacca gaagcatgaa taaacttttc gcgttttttt cgcagttcga aattctgacc
     3841 gagatatacc ttgcgtacca tctcatcagc agcaagttcc tcagctgtac cggcttttag
     3901 tatcgagcct tcgaacagca ggtatgacct gtcggttatc gctaaggttt cgtgtacatt
     3961 gtggtcggta atgagaatgc c
//
LOCUS       DAOPUW010000099         3921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_23394_length_3921_cov_3.424470, whole genome shotgun sequence.
ACCESSION   DAOPUW010000099 DAOPUW010000000
VERSION     DAOPUW010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23394_length_3921_cov_3.424470"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..1085)
                     /locus_tag="PKJ43_02625"
     CDS             complement(30..1085)
                     /locus_tag="PKJ43_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71483.1"
                     /translation="MKKILGLDLGTNSIGWALVNADEDGKPCSIAGMGSRIIPMDQAT
                     LSDFDKGNSKSQTAERTAFRGVRRIRERYLLRRDRLHRVLYRIGFLPEHYVQSIDFEK
                     RLGQFRSGAEPKIAWQKNDNGQYEFLFQNSFNEMLADFKVHQPQLLEEDKKVPYDWTI
                     YFLRKKALNYKIEKEELAWVLLNFNQKRGYYQLRGEDEEENPNKLVEFYALKVVAVEA
                     TDEKKGKDIWYNVHLENGWIYRRTSNIPLDWVGKTKEFIVTTDLNDDGTLKLDKDGKE
                     KRSFRAPKEDDWTLVKKKTESDIDQSKKTVGWYIYDTLLQKPDQKIKGKLVRTVERKF
                     YKTELKAILEKQKEFHA"
     gene            complement(1639..2172)
                     /locus_tag="PKJ43_02630"
     CDS             complement(1639..2172)
                     /locus_tag="PKJ43_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNZ71484.1"
                     /translation="MDFAELVKKRQSDRKYAVTPVEEGKIAQCIESARLSPSSNNSQP
                     WKFIVVDEPSLIEQVAQCADSLGMNKFTHQVPVIVAVVIERQNLLSSAGSLVQQKDYS
                     LIDVGMAVNQFCLQAADLGLGTCIIGWFDEKKLKKILGVPRSLRLPLLISLGYSEASP
                     RTKVRKPTEKMSSRNHY"
     gene            complement(2248..2763)
                     /locus_tag="PKJ43_02635"
     CDS             complement(2248..2763)
                     /locus_tag="PKJ43_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HNZ71485.1"
                     /translation="MSIRFVTNEELYSQVIEPLVNATSFVWIGTADIKDLHVRHHGGV
                     KSFLAVLDTLAQKKVSIRLLHAKDPGPNFRRSFDSYPLLWKSMERQLCPRVHFKHIVV
                     DGKIAYTGSANLTGAGLGMKGVNTRNFESGFIVNDAPVVEQIMTQFDDVWMGKFCKTC
                     RRKAYCSDPIL"
     gene            complement(2794..3222)
                     /locus_tag="PKJ43_02640"
     CDS             complement(2794..3222)
                     /locus_tag="PKJ43_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNZ71486.1"
                     /translation="MFNKETEYALRSLVYIQYQNDRQQTPGIEEISKEIEAPQAYVAK
                     ILQRMVRLGFVNSIKGKGGGFRFDESGGELTLKELILATEGGTILNGCGFGMKHCNSD
                     NPCPLHDQYGPIRDAINKLVSEETIQSLARKENAAFKVAL"
     gene            3472..>3921
                     /locus_tag="PKJ43_02645"
     CDS             3472..>3921
                     /locus_tag="PKJ43_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF542 domain-containing protein"
                     /protein_id="HNZ71487.1"
                     /translation="MITETLKNKTIGQIVADDYRAAAIFKNAGIDFCCGGNQSLELAC
                     KEKNADAEKISVEIKELELSTPTFQHDFKNWDIAFLADYIVNTHHKYVFKTLPELVFY
                     TQKISEVHGENHPELKEVASLFHEINRELLQHLKQEEEVLFPAIKEVM"
BASE COUNT         1095 a          879 c          713 g         1234 t
ORIGIN      
        1 cttcttcaga ataaaatttc cataaaccat caagcatgaa attctttctg tttctcaaga
       61 atggctttca attcagtttt gtagaattta cgttcaacag tacgaacaag cttccccttt
      121 attttttgat cgggcttttg tagaagcgta tcataaatgt accacccgac agtcttttta
      181 ctttggtcaa tatccgattc agttttcttc tttaccaaag tccaatcatc ttctttaggt
      241 gcacgaaaag aacgcttctc ttttccgtct ttatccagct ttaatgttcc gtcgtcattg
      301 agatcggttg taacaataaa ctctttggtc tttccaaccc aatcgagcgg aatattactt
      361 gttctccggt aaatccatcc gttttcaaga tgtacattgt accaaatatc tttccctttt
      421 ttctcgtcag ttgcttcaac agcaacaacc ttcaacgcat aaaactcaac cagtttattt
      481 ggattttctt cttcatcttc acctcttaac tggtaataac cacgtttttg attaaagttc
      541 agtaaaaccc aggctaattc ttccttttca attttatagt ttaaagcctt ctttcgaaga
      601 aaatagattg tccagtcata aggaactttc ttatcctctt ccaatagttg gggttgatga
      661 accttaaaat cagccaacat ttcattgaaa gaattctgga aaagaaattc atattgacca
      721 ttgtcatttt tttgccaagc aatttttggt tcagctcctg aacgaaactg tcctaatctc
      781 ttttcaaaat ctatactttg tacataatgt tcaggtaaaa atccaatcct ataaagtact
      841 cgatgcaatc gatctctacg caacaaatac cgttctctaa tacggcgaac gcctcgaaat
      901 gcagttcttt ctgctgtttg tgattttgaa tttcctttat cgaaatcgct taaagtcgct
      961 tgatccatcg gaataatccg acttcccatt cctgcaatgc tacatggttt cccatcttca
     1021 tcggcattta ccagtgccca ccctatgctg ttggttccta aatccagtcc taaaattttt
     1081 ttcatggtat attgttttat tggtttatat catttagttt tggtgtacaa ttgaaagcaa
     1141 ttcacaataa ggattattcc gttgtgaaaa cattcaaggt ggtctttgcg gatcgccttt
     1201 tttgttgccc aactatctga cttcatcctc tttctacctg tgcctttctt tccccaacat
     1261 ggacttaatg cttttctctt tgctgatatt ccatccatta tagcacaccg gtacccgtaa
     1321 cagatattgc acgacacaat accgtcacaa cacttcttaa gcacatcaat atgatttatc
     1381 agggaaacgg tagatgattc acaactcttt ggggggcgaa ccatgattcc gtcgttgctg
     1441 tatgtggcga acgcaaatgg catgttgggc aattacaata atactgccgg gttttgttat
     1501 tgattttcaa aaccatcgac ccttaaaaat aataaaaaca ttgattgaaa agaaataatt
     1561 ccaattttaa ttatcaaaaa aataattttc aaatgtattc tgattgtcaa cacgctatat
     1621 tgccctgaaa tcaacaagtc aataatggtt acggctgctc atcttttcgg tgggtttgcg
     1681 aactttggtg cggggtgaag cctcggagta acctaaagaa atcaataacg gaaggcgaag
     1741 ggaacggggt acgcccaata ttttttttag cttcttctcg tcgaaccatc ctataatgca
     1801 ggtaccaagc cccaaatcgg cggcctgaag acaaaactgg tttacggcca tcccaacatc
     1861 gatcaacgaa taatctttct gctgaacaag gcttcccgcc gaagaaagca ggttctgacg
     1921 ctcgatgact accgcaacaa ttaccggcac ctgatgggta aacttgttca ttcccaaact
     1981 atcggcgcat tgtgcaacct gctctatcag tgagggttca tcaaccacga tgaatttcca
     2041 cggctgcgag ttgttcgacg aaggcgatag cctggccgac tcaatgcatt gggctatttt
     2101 gccttcttca acaggagtga cagcatattt tctgtcgctc tgacgttttt ttaccagttc
     2161 tgcaaaatcc atgattgttg ttttataatg ctaaatatac ttgttttttg tggttaacaa
     2221 tccacaaaaa aggctagata aagtacgtta caggattggg tcgctgcaat aggctttccg
     2281 ccgacaagtt ttgcagaatt tacccatcca aacatcgtcg aactgagtca taatttgttc
     2341 aacaacaggc gcatcgttca caataaaacc cgattcgaag tttcgggtat tcacgccctt
     2401 catccccaac cctgcaccgg ttaggttggc tgacccggtg tatgcaattt ttccatcaac
     2461 aacaatatgc ttgaaatgca cgcgcggaca aagctgccgc tccatacttt tccacagcaa
     2521 gggataactg tcgaaactgc gcctgaaatt cgggccgggg tctttggcgt gcaataagcg
     2581 aatcgacact ttcttttgag ccagggtatc gagaaccgcc aaaaaagact ttaccccacc
     2641 atggtgccgt acatgaagat ccttaatatc ggccgtgcca atccatacaa atgatgtggc
     2701 attaacaagc ggttcaataa cctgactata taattcttcg tttgtaacaa acctgatact
     2761 catttcttct tttgttagcg gtgaatttaa tgattacagc gccactttga aagctgcatt
     2821 ttcctttcgc gccaaactct gaatggtctc ttcgctcacc agcttgttga tggcatcgcg
     2881 aatggggcca tattggtcgt gcaacgggca cggattatcg gagttacaat gcttcatgcc
     2941 aaacccgcaa ccatttaata ttgtgccgcc ctcggtagcc aaaatcagtt cttttaaggt
     3001 cagttcaccc cccgattcat caaaacggaa tccgccccct ttgcctttaa tcgaattgac
     3061 aaagcccagg cgtaccatgc gttgtaaaat ttttgccacg taggcttgcg gcgcttcaat
     3121 ctctttcgaa atctcttcaa tgcccggcgt ttgttgcctg tcattctgat attgaatgta
     3181 aaccaggctt cgtaatgcgt attcggtttc tttgttgaac atggtattat agtttaaaca
     3241 cagcgacacg gagttaagaa tgctttagaa agcgattgaa aacaattgtt ttctactgct
     3301 tccaattgct ttcaattgta ttccattttt tactctgtgt tcatctctgt tttctctgtg
     3361 gtttctaaat ccaaaggtaa aacatttctt ttaaaaatta caagaagacc tttttgtctt
     3421 ttaataaata attcatacct ttgtatccgt aattaatgta aaaagcggag tatgataaca
     3481 gaaacattga aaaataagac gatagggcaa attgttgccg acgattatcg agctgcggct
     3541 atctttaaaa atgccggcat cgatttttgc tgcggtggaa accaatcgct tgagctggcc
     3601 tgcaaggaga aaaatgcaga tgcagaaaaa atatcggttg aaattaagga gcttgagcta
     3661 agcactccca catttcaaca tgactttaaa aattgggata ttgccttcct ggccgattac
     3721 attgtgaaca cgcatcataa gtatgtgttc aaaaccttac ccgagctggt gttttacact
     3781 cagaaaattt cagaagtgca tggcgaaaat catcctgaat tgaaggaagt tgcctcgctg
     3841 tttcacgaaa ttaaccgcga gttactccag catttaaaac aggaagagga agtgcttttc
     3901 cccgccatta aagaggtaat g
//
LOCUS       DAOPUW010000100         3906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_23516_length_3906_cov_2.932745, whole genome shotgun sequence.
ACCESSION   DAOPUW010000100 DAOPUW010000000
VERSION     DAOPUW010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23516_length_3906_cov_2.932745"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PKJ43_02650"
     CDS             complement(<1..214)
                     /locus_tag="PKJ43_02650"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNZ71488.1"
                     /translation="MKDPEDCKSIEEIRNEIDTIDNQFVKLIAKRAKYVDNAAKFKAD
                     VDSVKDEKRVARVIDSRRMLAAEHGVS"
     gene            complement(285..1235)
                     /locus_tag="PKJ43_02655"
     CDS             complement(285..1235)
                     /locus_tag="PKJ43_02655"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNZ71489.1"
                     /translation="MIQRMNAKMEISKHISSLLKFNECVIIPDFGSFISNYVPSQFDP
                     ASNAYLPPAKEIVFNSKITKNDGVLINHVAEAESISYTQAFQAVAKWVSNAFDALNDG
                     EKIELSEIGTLQFDANGSFLFSSSSTSPFAAAYGLTPIGFPKMIRPVYVSSYSERPAV
                     SAVNKRKNALRIAAGIALLVSLSLFPQKIKKELGSIGKSSVNPLEVLTTPATPATKIE
                     TEEIEKPIAAEVSQPEKNVAVEVKGNYTLVGGSFSVQENAQSYCSELQKEGNQAEIVL
                     MKDGRHRVIFNAYTTREEALEAMEYYRASHPGSQVWVSTR"
     gene            2050..3438
                     /gene="glmM"
                     /locus_tag="PKJ43_02660"
     CDS             2050..3438
                     /gene="glmM"
                     /locus_tag="PKJ43_02660"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNZ71490.1"
                     /translation="MTLIKSISGIRGTIGGKPGEGLSPIDIVKFTSAYASFIQQKAGK
                     DNVKIVVGRDARMSGSIVNALVTNTLAAKGCNVVDIGLATTPTTEIAVPLENANGGII
                     LTASHNPKQWNALKLLNEDGEFLNDAEGKTILALAESGDFRYAPVDLLGKIEYKDYTS
                     THINSVLSLDLVNTELIKAANFSVAVDAVNSVGGIIVPKLLNMLGVNRIVEINCEPTG
                     NFAHTPEPISENLIDVASRVKAENVDVCFVVDPDVDRLVILNEDGSMFNEEYTLVAVA
                     DYVLSKTPGNTASNLSSTRALRDVTEKYGHSYVAAAVGEVNVVSAMKACNAVIGGEGN
                     GGVIYPASHYGRDALVGIALFLTLLAERKMKCSALRATYPEYFISKNKIELTPEIDVD
                     LILDKMKEKYAQEQINDIDGVKIDFEKSWVHLRKSNTEPIIRIYSEAATMKDADALAG
                     KIIDDIYAIIRS"
     gene            complement(3556..>3906)
                     /locus_tag="PKJ43_02665"
     CDS             complement(3556..>3906)
                     /locus_tag="PKJ43_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNZ71491.1"
                     /translation="VMCVCRRVAFHFTGLETLKIKETDRIHALITELKKVGAALYESQ
                     HGELAWDGQLDEEKFKSTAVIDTYHDHRMALAFAPIALTNGKIQINDPMVITKSYPGY
                     WSDLQKMGLIVEEA"
BASE COUNT         1074 a          869 c          822 g         1141 t
ORIGIN      
        1 gcgaaacacc atgttcagca gcaagcattc ttcttgaatc gattactctg gcaactcttt
       61 tctcatcttt tacagaatca acgtcggcct taaattttgc agcattgtcg acatactttg
      121 cccgctttgc aattagttta acaaattggt tgtcaattgt atcgatttcg tttctgattt
      181 cttcaatact tttacaatct tctggatctt tcattttcag agatataaat cataaaataa
      241 caagaaaaaa ggctcccgaa acggaagcct tgtgcgtgat atctttagcg ggtactgacc
      301 caaacttgcg aaccagggtg cgaagcgcgg taatattcca tagcctcaag agcctcttcc
      361 cgggtagtat aagcattgaa aattacccgg tgccgcccat ctttcatcaa cacaatctca
      421 gcttggtttc cttctttttg caattcggag caataagatt gtgcattctc ctgaacagaa
      481 aagcttcctc caaccaatgt gtagttaccc tttacttcaa cagctacatt cttttctggc
      541 tgagaaactt ctgctgcaat aggtttttct atttcttcag tttcaatttt tgtcgccgga
      601 gttgccggag tggtgagcac ttcaagcggg ttaacgctcg acttgcctat acttcccaat
      661 tctttcttga tcttctgtgg aaataaagaa agagaaacca ataatgctat tcctgcagca
      721 atcctaaggg cgttcttccg tttgtttact gcagaaactg ccggacgttc actgtacgaa
      781 gaaacgtata ccggccttat cattttaggg aaacctatcg gggttaagcc ataagctgct
      841 gcaaacggac tagtagaaga tgaactaaaa agaaaagaac cattcgcatc gaattgaagt
      901 gtgccaattt cagatagttc aattttttcg ccgtcattaa gcgcatcgaa agcatttgaa
      961 acccattttg ctactgcctg aaaagcttgc gtgtaactta tcgattcagc ctcggcaacg
     1021 tggttaataa gaactccatc gtttttggta atctttgagt taaaaacaat ttcttttgca
     1081 ggaggcagat atgcattgct ggccggatcg aattgtgaag gtacataatt cgaaataaaa
     1141 ctcccgaaat cgggaataat aacgcattca ttaaatttta gcaaagatga tatgtgcttc
     1201 gatatttcca ttttcgcgtt cattcgttga atcaaaagta gctattttta aaaggagcac
     1261 aaaggaaaaa atatcaacat tgttgaaaaa gtgttgaaaa catttttgag ttttgttgga
     1321 tatttcagaa caccagctat ttatgttttt tccttcttcg tattattatt tattcgaatg
     1381 cggggagctg tggtttcaat gtgtagcagc ttaatccttg tttctttcgc ctctagccgg
     1441 ctgggctctt ccttttgtga actaattaat ttgtttttta aagtgttcac gatttcgcta
     1501 cgcttcaatt gcaatcgata aaaaggaagt aaaaatcttt gtccaaatta agctgcacaa
     1561 cgctcctcgc caatcccgcc gacccacggg cttctggtac gcaagattgg cttcgtcccg
     1621 ctggcgcggc ccgcaatttg gacggccaac ccaccgcttt tgcgaaatat tggcgaggat
     1681 tcccttcgac tgacaaaaac aactgcgttc aggatgatcg caagggcgta gtggctgaaa
     1741 gtgctatccg atgagattac ttctgcaccg cctgacggca gtataattgt ccattgtcaa
     1801 ttctcaattg tcaattcctt cgccccgcac gcgttgccga ggccgatcct ttcatgaact
     1861 agtgtgggct ttccgagaaa tcgttaatct tcaatcgttt ttaatcgttc atgcgatgca
     1921 atcgtccgat agtaggctgg ccgttgttag gtctgtcata tttacctttt ctaaacaaat
     1981 aataaaacaa gcccaacttt ttcacctgta agtctatttt gttaatttcg catttttaaa
     2041 aactgaaaaa tgacgcttat aaaatctatc tcaggtatcc gtggaacaat tggagggaag
     2101 ccaggtgaag gtctttctcc tatcgacatt gtaaaattta cttcggctta tgcttccttt
     2161 atccaacaaa aagcagggaa agacaacgtg aaaattgttg ttgggcgtga tgcccgcatg
     2221 tcgggatcaa tagttaatgc cttagtaaca aataccctgg cagctaaggg ctgcaatgtt
     2281 gtagatattg gcctggccac aacacctaca accgaaattg ccgttccgct cgaaaatgcc
     2341 aatggcggga ttattcttac cgcaagccat aacccaaaac agtggaacgc gctgaaactc
     2401 ctcaacgaag acggagaatt tctgaacgat gcggaaggaa aaacaatcct ggcccttgcc
     2461 gaaagtggtg attttaggta tgccccggta gatttgcttg gaaaaattga atacaaagat
     2521 tacacctcta cccatatcaa tagcgtgctg tcgctcgacc tggtgaatac agaactgatc
     2581 aaagcggcaa acttcagcgt ggctgtcgat gccgttaatt cagttggcgg aatcatcgtt
     2641 ccaaagctgc ttaatatgct tggcgttaac cgcattgtgg aaataaattg cgaacctaca
     2701 ggaaactttg cccacacgcc cgaaccaatt tccgagaacc ttatcgatgt tgccagcagg
     2761 gtgaaagcag aaaatgttga tgtttgcttt gttgttgacc ccgatgtgga ccggttggta
     2821 atcctcaacg aagacggttc aatgttcaat gaagaataca cccttgttgc tgttgccgat
     2881 tatgtattat cgaaaactcc cggcaatacc gcttcaaacc tctcctcaac ccgggccctg
     2941 cgcgatgtta ccgaaaaata cggacatagc tacgttgctg cagccgttgg cgaggttaat
     3001 gttgtatcag ccatgaaagc ctgcaacgcc gttatcgggg gcgaaggcaa tggcggggtt
     3061 atctaccctg cttcgcatta cggacgcgac gcgttggttg gcattgcgct gttcctgacg
     3121 ttactggccg agcggaaaat gaaatgttct gccttgcgtg ccacctaccc cgagtatttt
     3181 atctcgaaaa acaagataga attaactcct gaaatcgatg ttgacctcat cctcgataaa
     3241 atgaaagaaa agtatgctca ggagcagatt aatgacatcg acggagttaa aatcgatttt
     3301 gaaaagagct gggtacacct gcgcaagtcg aataccgaac ccattatccg catctactca
     3361 gaggctgcga ccatgaaaga cgctgatgca ctggccggta aaattattga tgacatttat
     3421 gccatcatcc gcagttaata aaatcagcgg gtgaaataaa tgttattgat tttctgctaa
     3481 ccgggaaata ctaggaataa ggcagctaat tattcatagc gatgaattta aagttattca
     3541 tcgggctttg tggtgctatg cttcctcaac aatcagcccc atcttttgca gatcgctcca
     3601 ataaccggga tatgatttgg taattaccat cgggtcgtta atctggattt taccattggt
     3661 aagcgctatg ggtgcaaaag caagcgccat gcgatgatcg tgataggtgt cgataacggc
     3721 agtgctttta aacttctctt catctaactg tccatcccat gctagttccc catgctgtga
     3781 ttcgtataat gctgccccta cttttttgag ttcggttatc aatgcatgaa tcctgtcggt
     3841 ttcttttatt ttgagcgttt cgagccctgt aaaatgaaaa gcgacccgcc ggcaaacgca
     3901 cattac
//
LOCUS       DAOPUW010000101         3889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_23659_length_3889_cov_3.329943, whole genome shotgun sequence.
ACCESSION   DAOPUW010000101 DAOPUW010000000
VERSION     DAOPUW010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23659_length_3889_cov_3.329943"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..1040)
                     /locus_tag="PKJ43_02670"
     CDS             complement(279..1040)
                     /locus_tag="PKJ43_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71492.1"
                     /translation="MKTRDIAIIKKGFLLVSFRLSRLNNFMLAIFFILIVGNAFGAGS
                     RPQDKVRLTGSAYVFPLGLEGTPYLNQDWSFADIVLENGMVAVDEKVKFNMVTNDLIF
                     YNEDLKRIFILDKETVKEFRIKNGKETTNTFVKYLGSAVGFKLKTNDFVEFLAGDQLR
                     FNVRHSADIVEANDVNSKNKVYPKKYYYVEFDGKATPVNLRLRSIYRLFPGQKKNIKQ
                     LVHQNKLKRSGEKNVKKLIDLLNNEPEILKSLVID"
     gene            complement(1136..2794)
                     /locus_tag="PKJ43_02675"
     CDS             complement(1136..2794)
                     /locus_tag="PKJ43_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71493.1"
                     /translation="MNSKIVCALVLLLISGFLKNANAELSPEVTLFQLQFNKNIFVSG
                     EKIWFKNSIVAGHETEHQTILYADLCSEGQIINSCIVQRNNNHWQSDLLIPDSLSTGV
                     YLFRAYTGNYNGKPEMVSRLVTVINRFGNNTTNESRKLEPGYMPFDNINVLPADLGSE
                     IKLTAAKNRVAPNSSVVFTLNANPDKYISGVSLSVYKVPEENALSSAQLQSEKKVISS
                     VADHVKIYNTLTLSGIVTNSQTNEPVVGEVVLFSVPDTVPQINYAVTDSKGEFIFNVD
                     GYYGRQDVIIQTLSKDSVYQIKVYPNLLTPPLKIPFFVPSEVEQSEFVKMAVDRQLLA
                     KAYTGESNEVVKKPQFKYPFYGQTDIVTIPSMYVDLVDFEEISKEILPLCRIRKEKNS
                     ASLRLYDPDKLSFLESPWVIVDGVPVFDVKLLFPLNSPAIEKIVTQKQIRCYGALYIE
                     GALSVFTTKGGYTDMPLPANAVRLFFDTFRYPQEFKKYKVEPNSALPDFRDVLVWEPM
                     LDCQSATCSHTVSTSYEKGNYIAVAEAIGRDGEVQRTVLKFIVK"
     gene            complement(2805..>3889)
                     /locus_tag="PKJ43_02680"
     CDS             complement(2805..>3889)
                     /locus_tag="PKJ43_02680"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HNZ71494.1"
                     /translation="KFYQYILLLLVLALASCEELYTPEIEESPSALVVEGLLTDRNEY
                     VTIKLTRSASFNDNSFYYSERGAIVTIESLSGRVYSTSEVSKGNYQTNEPVKTEAGQG
                     YYVRIITRDGTEYRSEEEVMPVPSQIQDVYVVDSTFRDLNYNYWGEPVVSDYRGITIS
                     IRPQQTGAENTGFLYRWNGLVNYLVYSELQLSGFNYYCWDKIWSTTTYVYDYFKDEYI
                     TELPVGDLHSLSFYYLGPLPIDSTRFEGEIQRINTTSLYYRLEQYSISKNGAKYWRSI
                     KKQSESSGTLFDPVEEQIIGNISCLSDPCKIAFGYFSVVSASEKVIAVDFDGEKCEEV
                     RVVDSMPNPDHDEDCSLNEIPDFWFN"
BASE COUNT         1200 a          822 c          719 g         1148 t
ORIGIN      
        1 aaggccaaca aatataaaca tgaacagatg agaaaacaaa cataaaaaaa tcggctatac
       61 cacacccata gcgggtttta gaagatttta aaaggatttt aacgtatttt tcgtcatgct
      121 ttctttgccc ataaaggctt caaaacaata aacctgatgg gtcaaatttt ccctttgcct
      181 gaaaaaattt ttgaaatttg gaatgaattc agataatttc agtttggatc gataaaaata
      241 aaccacccaa accttcagga aaacagcact aaataagatt aatcgatcac aagtgatttc
      301 aaaatttcgg gttcattatt gagcaggtcg atgagctttt ttacattttt ttcaccagat
      361 cttttcaact tattctggtg caccaactgc ttaatgtttt ttttctgccc ggggaaaagg
      421 cgatatatag atctcaggcg aagattaacc ggtgttgctt tgccgtcaaa ttctacataa
      481 tagtattttt ttgggtatac tttatttttt gaattcacat cgttcgcttc aacaatatct
      541 gccgaatgcc ttacattgaa acgcaattga tctcctgcaa gaaattcaac aaaatcgttg
      601 gttttcaact taaatccaac ggcactccct aaatatttta caaaagtatt ggtggtttct
      661 ttcccgtttt ttatcctgaa ttcttttacg gtttctttat cgagaataaa aatccgcttc
      721 aggtcttcgt tgtaaaaaat caaatcgttg gttaccatat tgaatttcac tttctcgtca
      781 acagcaacca tcccgttttc cagcacaatg tcggcaaacg accaatcctg gtttaaataa
      841 ggtgttcctt ctaaccctaa aggaaagaca taagcactgc cggtaaggcg aaccttatcc
      901 tgaggccttg aaccagctcc aaaggcattc ccaacgatga gaataaaaaa gatggccaac
      961 atgaaattgt taagtctgct taagcggaaa ctgaccaaca aaaatccttt ctttattatc
     1021 gctatatctc tagttttcat atctttattt actttcttct aataaaaacc tcaccacaac
     1081 aacctggccg aagtataaat ttaataacaa aaagtaccct tcaataagta gctaactatt
     1141 taactatgaa tttcaatact gttctttgca cttccccatc acgaccaatc gcttcggcta
     1201 cagcaatgta gtttcctttt tcgtaagagg tacttacggt gtgcgaacag gtcgccgact
     1261 gacaatcgag cattggttcc caaaccaata catcgcgaaa atcgggcaat gcagaattag
     1321 gttcaacttt atattttttg aattcctgcg gatacctgaa tgtatcgaaa aaaagacgta
     1381 cagcattggc tggcaacggc atatcagtat agcctccttt cgttgtaaac acagaaagtg
     1441 caccctcgat atatagcgcg ccatagcaac gaatctgttt ttgtgtcaca atcttttcta
     1501 ttgccggaga atttaatggg aaaagaagtt ttacatcgaa aactggcacc ccatcgacaa
     1561 taacccaagg cgactcgaga aatgataatt tatcgggatc gtataaacgt agcgaagccg
     1621 aatttttctc tttacgaata cggcacaacg gcagtatttc ttttgaaatc tcctcaaaat
     1681 caaccaaatc tacatacatt gatggaattg taacgatatc tgtttgccca tagaaagggt
     1741 atttaaactg tggttttttg actacttcat tgctctcgcc tgtataagct ttagccaaca
     1801 actggcggtc aacagccatt ttcacaaact ccgactgttc gacctctgat gggacaaaaa
     1861 acggaatttt caagggcggg gtaagcaggt tgggatagac ttttatttga tatactgagt
     1921 ctttcgaaag cgtttgaatt atcacgtctt gtcgtccata atatccgtca acattaaaaa
     1981 tgaactcccc tttgctgtcg gtgaccgcat aattaatttg aggtacagtg tcgggtacag
     2041 aaaagagcac aacctcgcca accacaggct cattggtttg agaattagtg acaataccac
     2101 taagcgttaa ggtattgtat attttcacat ggtcggcaac cgaggaaatt acttttttct
     2161 cggattgcag ttgggcgctg gataaggcat tttcttcggg gactttataa accgatagag
     2221 aaaccccgga aatatattta tcgggatttg cattaagagt aaaaactaca gaagaatttg
     2281 gagctacacg atttttcgca gcagtcaact taatttcgga gccaagatcg gcaggtaaaa
     2341 cattgatatt atcgaaaggc atgtagcctg gctcgagttt tctcgattcg ttagtcgtgt
     2401 tatttccaaa tcggttgatt acagtcacca gcctggaaac catctcgggt tttccgttat
     2461 aattccctgt gtatgcccta aatagatata ccccggttga aagtgaatcg gggatcagca
     2521 aatcagattg ccaatgattg ttattgcgct gaacaataca cgaattgatg atttgcccct
     2581 cactgcacaa gtcggcatac aatatggtct gatgttcagt ttcgtgacct gcaacaattg
     2641 agtttttgaa ccatattttc tcacccgaaa caaaaatgtt tttattaaac tgtaactgaa
     2701 acaaggttac ttcaggagat aattcggcat tagcattctt taagaagcca gaaattaaca
     2761 ataatactaa cgcgcaaaca attttagaat tcattataaa tcgattaatt aaaccaaaaa
     2821 tcgggtattt catttaaact gcaatcttcg tcgtgatcgg ggtttggcat actgtctacc
     2881 acacgaacct cttcgcattt ttccccatcg aaatcgactg caataacctt ttcagatgct
     2941 gaaacaacgc taaaataacc aaaggcaatt ttgcacggat cgctaagaca agagatattg
     3001 ccaatgatct gctcttctac cgggtcgaaa agtgttcccg acgattcact ttgtttctta
     3061 atactgcgcc aatattttgc cccatttttc gatattgaat actgttcaag cctataataa
     3121 aggctcgtgg tattaattct ctgtatttca ccctcaaatc gagtcgaatc gatgggtaaa
     3181 gggccaagat aatagaacga aagagaatgc aaatcgccaa cgggaagttc tgtaatatat
     3241 tcatccttga aatagtcgta cacataggtc gttgtcgacc atattttatc ccagcaataa
     3301 taattaaagc cagaaagttg cagttcagaa tagacaagat agttcactag tccattccaa
     3361 cgatagagga acccagtgtt ttcagcgcca gtttgttgag gcctgataga aatagtaatt
     3421 ccacgatagt cggaaacgac aggctcaccc caatagttat aattcaggtc gcggaatgtt
     3481 gagtcgacca cgtaaacatc ctggatctgg ctcggcacag gcatcacttc ttcttcagac
     3541 cggtactcgg ttccgtcgcg ggtaatgatg cgaacgtaat acccctggcc agcttctgtc
     3601 ttcaccggct cattggtttg ataattccct tttgaaactt cggaggtaga gtatacccga
     3661 ccggaaagcg attcgatagt aactatagca ccacgctcgc tgtaataaaa tgaattatcg
     3721 ttgaacgaag ccgaacgggt aagcttgatg gttacgtact cgttcctgtc ggtaagtaaa
     3781 ccttcaacca ccagcgcact tgggctctct tctatctcgg gcgtgtacaa ttcttcacaa
     3841 gacgcaagag ctaaaaccaa aagcaataaa atatattgat aaaatttcc
//
LOCUS       DAOPUW010000102         3878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_23749_length_3878_cov_3.542244, whole genome shotgun sequence.
ACCESSION   DAOPUW010000102 DAOPUW010000000
VERSION     DAOPUW010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23749_length_3878_cov_3.542244"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..387)
                     /gene="rpsI"
                     /locus_tag="PKJ43_02685"
     CDS             complement(1..387)
                     /gene="rpsI"
                     /locus_tag="PKJ43_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNZ71495.1"
                     /translation="MEVINALGRRKAAVARVYVKEGKGTITINDREFKEYFPQETLQY
                     VVLQPLNLLNVAQQYDIKVNLDGGGFNGQAEALRLAISRALVKIDAESKSELKKAGFM
                     TRDPREVERKKPGQPKARKRFQFSKR"
     gene            complement(387..842)
                     /gene="rplM"
                     /locus_tag="PKJ43_02690"
     CDS             complement(387..842)
                     /gene="rplM"
                     /locus_tag="PKJ43_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNZ71496.1"
                     /translation="METISYKTVSANSATVTKEWVVVDAEGQVLGRLASKVAKILRGK
                     NKPSYTPHVDCGDNVIVINAEKVILTGKKLTDKEYVRHSGYPGGQTFQSAEDILKKYP
                     ERLVEKAVRGMLPKNRLGRELFRNLRVVIGENHDHEAQKPRVIDLNSIR"
     gene            complement(1129..1785)
                     /gene="upp"
                     /locus_tag="PKJ43_02695"
     CDS             complement(1129..1785)
                     /gene="upp"
                     /locus_tag="PKJ43_02695"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNZ71497.1"
                     /translation="MELINLGAQNSIFNQFIAEIRDVNIQCDPLRFRKNIERVGEIFA
                     YEISKRIDYTEREVTTPLGVARVNIPTGFPILATILRAGLPLHQGLLNYFDKSGNAFI
                     SAYRKYIDQKDFVIEFEYIASPSLDDKVVILSDPMLATGSSLEISYQALLQKGKPRHV
                     HLCAIIASQAGVNYVQNHLPSDNCTLWVGAIDPKLNDHAYIVPGLGDAGDLAYGEKID
                     "
     gene            complement(1772..2560)
                     /locus_tag="PKJ43_02700"
     CDS             complement(1772..2560)
                     /locus_tag="PKJ43_02700"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNZ71498.1"
                     /translation="MKTAKETDIVFDRRKPLAKRLIKNTLSAISAAALHGNKVRCNIC
                     QHTFRRFIKASNMSQLNGKCPICGSKAADRILWLYLQNEVTASKTKKRHLFFEHNTFI
                     AEKILNMDLQVDIVSFEGTFQADKIFNEKIKERKYDVIIFAHLLEKTEDEQYALAKLR
                     NLLRNGGYLLIMTIVNQHMDRTYEKPATSEDKERLGRFYVRGVKRVYGSNVAKHLAKA
                     GFEVEAIDYADQLGNAARTYYQLGEDSEEIIYKCKKIDKQYGTN"
     gene            complement(2655..3737)
                     /locus_tag="PKJ43_02705"
     CDS             complement(2655..3737)
                     /locus_tag="PKJ43_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71499.1"
                     /translation="MTLRKVTISELTTFVESGEYLKYGILPITPLRAISQANNPKAKP
                     DDVVLTLSLGDDGQLQGFIGALPDNIKNEHCAWNSCWWVKPGCSAETSLKLFLSFLTD
                     WNKKVLFSELTPHTNSLIEKLKFCNHQTIRGLRGYTRFCLADILPRKKSWTKKIRWLL
                     RITDTIANIFMPLIFASNPLPKAIHIEAIDFPKEDDNAFIEKHNTLQPAKRYSEDYKW
                     IKKFNWIETFQKGAGNSPVNYPFSYRTKRFEMQWLRFSYEDRTVALAAYTLRDKELKL
                     PCIYAEKEFIPQIGDYFYNMLLKDKCLCSATTFDPDLANYLLTKKKFIFKTRLPKYTA
                     ISKALSESETIERFIMQMGDGDAIFT"
BASE COUNT          955 a          778 c          804 g         1341 t
ORIGIN      
        1 ttaacgtttc gagaactgga agcgcttgcg tgcttttggt tgtcccggct ttttacgttc
       61 aacttcacgt gggtcgcggg tcatgaatcc agcttttttc aattctgatt tgctttcagc
      121 atcgatttta accaaagctc tcgagatggc taagcgaagt gcttcggctt gaccattgaa
      181 tccacctccg tcgaggttta ctttgatgtc gtattgctga gcaacattta atagatttaa
      241 cggttgaagc acaacgtatt gaagtgtttc ttgtggaaag tattccttga attcacgatc
      301 gttgattgtg atggttcctt tgccttcttt tacgtaaaca cgggcaacgg cggctttcct
      361 tcttcctagt gcgtttatta cttccattat ctaatgctgt ttaaatcaat aactctgggt
      421 ttctgtgctt catggtcgtg gttttcgcct attactaccc taaggtttct gaatagttcg
      481 cggccaagcc tgttctttgg taacatgcct ctaactgctt tttcaaccaa acgttctgga
      541 tatttcttta aaatatcttc agccgattga aatgtttgac ctccaggata ccctgaatgg
      601 cgaacgtatt ctttatcggt gagtttttta ccagtaagga ttactttttc ggcattgatg
      661 acgattacgt tgtcgccaca atctacatga ggggtataac taggtttgtt tttacctctg
      721 agtatttttg ccactttgct tgccaaacgg cctaaaacct gaccttcggc atcaactaca
      781 acccactctt ttgtaacagt ggcgctgttg gctgaaactg ttttataact tattgtttcc
      841 acttgaatta caagtattaa aattagtacc aattaattac tttgacttgc ctttcgtgat
      901 gtgtcgcctg taatttgttg cgctaggact gtatttacgt cttaattacc gacagtaaaa
      961 tcaacaaatt accacatagc aatgtcgcct gataatcggg actgcaaaaa tattttcttt
     1021 tttttgaatt ccaacaggtt tgctgaaatt tatttgtcgt tttgctgaat ttgattttga
     1081 cttaaactct gcaccttgtt ttcttatctt tactattgca ggtgtttgtt aatcaatttt
     1141 ttcgccatag gctaaatcgc ctgcatcacc aagcccggga actatgtagg catggtcgtt
     1201 caacttaggg tcgattgccc caacccaaag cgtacaatta tccgacggaa ggtgattctg
     1261 tacgtagttt accccggctt ggctggcaat aattgcgcat aaatgaacat gtctgggttt
     1321 ccctttctgc aacaacgcct gataacttat ctcgagtgat gagccggttg ccagcatcgg
     1381 gtctgagagg attaccactt tgtcgtcgag cgatggtgag gctatgtatt caaattcaat
     1441 tacgaaatct ttttggtcga tatatttccg gtaagccgaa atgaatgcat tcccgctttt
     1501 gtcaaaataa ttgagaagtc cttgatgaag cggtaagcca gcccgcaaaa tggtggcaag
     1561 tatcggaaac ccagttggga tgtttacccg ggcaacccct aatggcgttg taacttcgcg
     1621 ctctgtatag tcgattcgct tgcttatttc atacgcaaag atttcgccca ccctttcaat
     1681 gttttttctg aaacgaaggg ggtcgcattg gatattcaca tcgcgtattt ctgcaatgaa
     1741 ttgattgaaa attgaatttt gtgccccgag attaattagt tccatactgt ttatcaattt
     1801 ttttgcattt gtatattatt tcttccgagt cttcacccaa ctgataatag gtacgtgcgg
     1861 cgtttcccaa ctggtctgca tagtcgatgg cttctacttc aaatcctgct tttgccagat
     1921 gttttgccac attgctgcca tatactcttt tgactccacg tacgtaaaat cttccaagtc
     1981 gctctttatc ttcgcttgtt gcgggctttt cgtaggtgcg gtccatgtgt tggtttacta
     2041 tggtcattat caacagatac cctccattgc ggagcaaatt tcgcaacttc gccagggcgt
     2101 attgctcatc ctcggttttc tccagcaaat gtgcaaaaat tattacatcg tattttcttt
     2161 cttttatttt ttcgttgaag attttgtctg cttgaaaagt gccttcgaaa gaaacgatgt
     2221 cgacttgcag atccatgttt aatatttttt ctgcgataaa cgtgttatgt tcgaaaaaga
     2281 ggtggcgttt tttagttttc gaagcggtta cttcattttg aaggtatagc cacaaaatcc
     2341 tgtctgctgc ttttgaacca catatcgggc acttaccatt tagctgactc atgttcgagg
     2401 ctttaataaa acggcgaaag gtatgctggc aaatgttgca gcgcactttg ttcccgtgca
     2461 atgcagccgc acttatcgca ctaagagtgt tctttatgag cctctttgca agtggttttc
     2521 ttctgtcgaa aacaatatcc gtttctttgg cggttttcaa atcgtaatat tgtttgttgt
     2581 tctgatcctg aaatatttat ggtataaaaa tacaggatta ttctactcgt cgcagaattt
     2641 ttcggaagaa gtttttacgt gaaaatagca tcgccgtcgc ccatttgcat aataaagcgt
     2701 tcaatggttt ctgattctga taatgctttg gatattgccg tgtattttgg cagacgggtc
     2761 ttgaaaatga attttttctt tgttaaaagg tagtttgcca aatccgggtc gaatgtagtt
     2821 gctgaacaca agcatttatc ttttaaaagc atgttgtaga agtagtcgcc tatttgagga
     2881 ataaactctt tctcggcata aatacagggt aattttagtt ctttgtcgcg aagggtataa
     2941 gcagccagtg caacggttct gtcctcataa ctgaaacgca accattgcat ttcaaagcgt
     3001 ttggtccgat aggaaaaagg ataatttacg ggcgagttgc ctgccccttt ttgaaaggtt
     3061 tctatccagt taaacttttt tatccactta taatcttctg aatatctttt tgcgggttgc
     3121 aatgtgttgt gcttttcaat aaacgcgttg tcgtcttctt taggaaaatc aattgcttca
     3181 atgtgtattg ctttgggaag aggatttgaa gcgaaaatca atggcatgaa aatattggca
     3241 attgtatcag taatgcgtag cagccatcta atcttctttg tccatgactt ttttcgggga
     3301 agaatatcgg caagacaaaa acgggtgtag ccacgtaatc ctcttattgt ttgatggttg
     3361 caaaatttca atttttcaat caggctattg gtgtgcgggg tgagttccga aaacagtact
     3421 ttcttgttcc aatcggttaa aaacgagagg aatagtttta gcgaagtttc ggctgaacag
     3481 cctggtttca cccaccagca agaattccag gcgcaatgtt cgtttttgat attgtcggga
     3541 agggctccaa taaacccttg gagctggcca tcatcgccaa gtgatagggt gagtaccacg
     3601 tcatcgggtt tggctttggg gttatttgcc tgactgattg cgcgcaatgg ggtaatcggg
     3661 aggataccgt attttaggta ttcaccactt tcaacaaatg ttgtcagctc tgatatggtg
     3721 accttcctaa gtgtcaatta attgtttttt agctgtttcg aaactgaagt gcaaatgtct
     3781 ccaatcgttt ttatttccag aacttccata aagtcgatac ttatagcaaa ttctttttca
     3841 acttctgaaa taatcattac atgtcgtagc gaatccca
//
LOCUS       DAOPUW010000103         3866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_23829_length_3866_cov_3.427972, whole genome shotgun sequence.
ACCESSION   DAOPUW010000103 DAOPUW010000000
VERSION     DAOPUW010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23829_length_3866_cov_3.427972"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3462)
                     /locus_tag="PKJ43_02710"
     CDS             complement(<1..3462)
                     /locus_tag="PKJ43_02710"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNZ71500.1"
                     /translation="MLVIISLLFLTLAIGYGIFQTSYFQKILINKITAGISKSIDADV
                     SIGRVKFSLFTKVVLDDFYLSDQNKDTLLYTSKLVASIDSLSLKNNALHFKSITLDNP
                     KVMVEKTGDSIYNFSFLFGEKQPSQNKPWSASISMLEIEDGIFCYSDTSMSESLSDFV
                     HLSKINLSLDKLKFPSSKRFAFNLNQLSFQCRNGFAINEFSSKVSYTDSIFDLQSISV
                     ASDNSLLKVDSLHLDLKEYLLAKNIFDLRFDSYIERIKLDYKDIAFLLSDYDGNSVAA
                     EISGRIYGKLSEIKGKNVKARLEDLTRLNGDFYINGLPDIENTYLFVNLFESYANLIE
                     LRNLKLPKKIFPVEPNMPKFLDNLGVFTYKGNFTGFLNDFVAYGTAYSNLGTVDGDVS
                     FQPDKNQSLKVKGRINTRNLRIGSLFNTEYLGKLTLSGKIDGSIRKSNYDFTFNGIVD
                     TIDVFNYKYKNIEIKGNIKNNHFDGALSIDDPNLKMNYLGSLDISPNMPTFRFKSQID
                     YADLQKLNLLKTDSIAKISGVIDANFEGKDLDMIKGVVDVKQLRFKNSKAIMFLENAQ
                     LKNDKVDSTNILSLRSDWADIDISGHYSFKTLNNSLINLYEIYFPSSLPLTRERTYTQ
                     NRFLFTAGIKNTDALTKAFLPSLVLEKPFTIKGFFDETNKRAHVETILPVIKYAKQEA
                     DNLSINVEATPEQIRFDLLAETYKMTEKVETKNLTINSTGKNDMVNLDVHWENQDGTK
                     NLGKLKTVTTFERTETKYPHIEVKMLPSWFIISDSLWSIDQSVISIDSTSFDISQLLL
                     KNGEQRIKLWGNINENPASKAKINLQNIDIRLIDLLIGQSGLGGKINGNLDITDLYKK
                     KKINLDLIINSLSLNQATFGDLSVKSDWSPELEKLIAKLTLKDEDRTLIDGAGAIDPM
                     KSLVDFNLNLDKAPLKFLELLTPFLFYDISGEVNGRMRVSGKIDHLMFNGKLTPETRI
                     GMGITPTKSHYYFSDPVVFKADSILFPAMRIEDEMGNNGIFSGHIAHQSFGKMRYNLT
                     VDTDKLMAMNISSSDNEYFYGTAFVSGFLKINGYDSDVSLVGNLKTARGTNINIPLDT
                     KSTIEHYDFIRFTNVKEEIEQTEYKLVSNGLNMNFDIDINPEAKVQLIFNSQMGDIIK
                     GEGNGNLQ"
     gene            3567..>3866
                     /locus_tag="PKJ43_02715"
     CDS             3567..>3866
                     /locus_tag="PKJ43_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNZ71501.1"
                     /translation="MLHNNKVIILGIESSCDDTSAAVIENGVIRSNVIASQKVHEQYG
                     GVVPELASRAHQHNVVPVVDVALKQASIDASQLTAIAFTRGPGLLGSLLVGTSFAK"
BASE COUNT         1117 a          701 c          738 g         1310 t
ORIGIN      
        1 ctgcaaattc ccatttcctt cgcctttaat aatgtcgccc atttgcgaat taaatatcag
       61 ctgaacttta gcttcgggat tgatatcaat gtcgaagttc atatttaatc cattggatac
      121 cagcttgtat tccgtttgtt caatttcctc tttgacattg gtaaagcgga taaaatcata
      181 atgttctata gtgctttttg tgtcgagtgg aatgttaatg tttgttcctc tggcagtttt
      241 taaatttccg acaagagaaa catcggaatc gtatccatta attttcagaa atccacttac
      301 gaatgctgta ccataaaaat attcgttatc agacgaagag atgttcatcg ccatcagttt
      361 gtcggtgtca accgttagat tatatcgcat ttttccaaag ctttggtggg caatgtgccc
      421 gctgaaaatg ccattgttcc ccatttcatc ttcaattctc attgcaggga aaagtattga
      481 atcggctttg aaaacaacag gatcggagaa ataatagtgc gatttggttg gtgttatgcc
      541 cataccaatc cgagtttctg gcgttagctt cccattgaac attagatgat cgattttccc
      601 cgaaactctc attcttccat ttacttcgcc gctgatgtca taaaataaaa acggtgtaag
      661 cagctccaaa aattttaatg gtgctttgtc aaggttaagg ttaaaatcaa ccaaactctt
      721 cattgggtct attgctccgg ctccgtcaat tagcgttctg tcttcgtctt ttaatgtcaa
      781 ttttgcaatg agtttttcaa gctcaggact ccagtcactt tttaccgaca aatcgccaaa
      841 agtagcttga ttgagagata aactattgat tatcaaatct aaattaatct ttttcttttt
      901 gtacagatca gtaatatcga ggttcccatt aatttttcca ccaagtccag actgaccaat
      961 gagcaggtca attaaacgga tatcaatatt ctgaaggttg attttggcct ttgatgccgg
     1021 attttcgtta atatttcccc atagttttat tctctgctca ccgtttttta acaataactg
     1081 gcttatatcg aacgatgtag aatcaatact gatgactgat tggtcaatac tccacaatga
     1141 atcggaaatg ataaaccacg atggtagcat ttttacttca atgtggggat actttgtttc
     1201 tgttcgttca aaagtggtaa cagttttcaa tttgcctagg tttttggtgc catcttggtt
     1261 ttcccaatga acatcaagat taaccatgtc gtttttgcct gtggagttga tcgttaagtt
     1321 tttggtttca actttttcag tcattttata ggtttcggct aacaagtcga atcgtatctg
     1381 ttcaggagta gcttcaacgt ttatcgaaag attatctgct tcttgtttcg catattttat
     1441 cacaggaagt attgtttcta cgtgtgcacg tttattcgtt tcgtcaaaaa atcctttgat
     1501 tgtaaacggc ttttctaata ccagcgatgg aaggaatgct ttggtcaggg catcagtgtt
     1561 ttttatacca gcggtaaaaa gaaaacggtt ttgggtgtat gtcctttcac gtgtaagagg
     1621 cagagaggat gggaaataaa tttcatataa atttatcaaa gaattgttta aggttttaaa
     1681 actataatgt ccgctgatat caatatcggc ccaatcgctt cttagcgaaa gaatgtttgt
     1741 gctatcaacc ttgtcattct tcagttgtgc attttcgagg aacatgatgg ctttcgaatt
     1801 tttgaagcga agctgtttta catcaacaac ccctttgatc atatcgagat ccttaccctc
     1861 aaaattagca tctattacgc ctgatatttt agctatagag tcggttttaa gcaagttaag
     1921 tttttgtaaa tctgcataat caatttgact tttgaatctg aaagtaggca tgtttggcga
     1981 gatatcaagg ctcccgaggt aattcatttt caggtttggg tcgtcaattg aaagagctcc
     2041 gtcgaagtgg ttattcttta tgtttccctt tatttcgatg tttttatatt tatagttgaa
     2101 aacatcaatg gtgtctacta taccattaaa agtaaaatcg taatttgatt ttcggattga
     2161 tccgtcaatt tttccactaa gggtaagttt tccaagatat tcggtgttga aaagcgaacc
     2221 aatgcgaagg tttcgggtgt ttatccttcc ttttactttt aatgactggt ttttatcggg
     2281 ctgaaaggat acatcgccat caactgttcc caaattactg tatgcagtac cataagctac
     2341 aaaatcgtta agaaagccag taaaattgcc tttataggtg aatactccta agttgtcgag
     2401 aaacttgggc atgtttggtt caaccgggaa aatcttctta ggaagtttaa ggtttcgcaa
     2461 ttcgataagg ttggcgtacg actcgaacaa gttgacaaac agataggtat tttcaatatc
     2521 tggcaagccg ttgatataaa agtcgccatt gagcctggta agatcttcga gccttgcttt
     2581 tacatttttc cctttaatct cgctgagttt tccgtatatc cgtcctgaaa tttcggctgc
     2641 gacactgttg ccgtcgtaat ctgacagaag aaaggcaata tccttgtagt caagcttaat
     2701 tctttcaata taagagtcga atcttaaatc gaaaatattc tttgctaaaa ggtattcttt
     2761 gagatcaaga tgcaatgaat caactttcag caacgaattg tcagaagcta cactaattga
     2821 ttgaaggtca aaaatgctgt ctgtatacga aactttggat gaaaattcat tgatggcaaa
     2881 accattacgg cattgaaatg agagctggtt caaattaaaa gcaaaccttt tgcttgaagg
     2941 aaattttaat ttatcgagcg acagatttat ttttgacaaa tgcacaaagt cgctcagcga
     3001 ttcgctcatc gaagtatcgg aataacagaa tattccgtct tctatttcca gcattgaaat
     3061 acttgcactc catggcttat tttgcgaagg ttgtttttct ccaaaaagga agctgaaatt
     3121 atatatacta tcaccagttt tttcaaccat tacttttgga ttgtcgaggg taattgattt
     3181 gaagtgaagc gcattatttt ttaacgacaa cgagtctatt gatgcaacca gtttgctggt
     3241 atatagcaag gtgtctttgt tttggtcact tagataaaaa tcgtcgagta ctacttttgt
     3301 aaataaggag aatttcactc gtccaatcga aacatcagca tcgatacttt tcgaaatgcc
     3361 cgcagtaatt ttattgataa gtatcttttg gaagtagctg gtttgaaaaa taccatagcc
     3421 aatcgccagt gttaaaaaca gtagcgatat aataaccagc aatatattta ttgttttttt
     3481 aatacttttg tcctcccaaa aataagatca tacaaaataa caatttttag accagaaatt
     3541 ggttctagtc aaagaaaata aagtatatgc tacataataa taaagttatc atacttggaa
     3601 tagaatcttc gtgcgacgat acttcggctg ctgttataga aaacggagta atcaggtcga
     3661 atgttatagc cagccagaaa gttcatgaac aatatggggg tgttgtgccc gaactggcct
     3721 caagggcaca tcaacataat gtggttccgg tggtcgatgt agcgctcaag caggcatcta
     3781 tcgatgccag ccagcttaca gccatagcat ttacgagagg tcccggactt ttgggctctt
     3841 tgcttgtggg cacttcattt gccaaa
//
LOCUS       DAOPUW010000104         3863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_23848_length_3863_cov_3.311975, whole genome shotgun sequence.
ACCESSION   DAOPUW010000104 DAOPUW010000000
VERSION     DAOPUW010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23848_length_3863_cov_3.311975"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PKJ43_02720"
     CDS             complement(<1..839)
                     /locus_tag="PKJ43_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71502.1"
                     /translation="MKKISGKALWARLSFIVLGLASTIWFLIRVIPKPSRATYPCMKA
                     AAPFMSGFVVYILSIAGSAFAIRLFHKKLQASKYVAATALLAATFVMIIAANVSTGQE
                     SKAATLVEASYFEANNPVGTADGLKPGRVVWVWNKNATNENFTPSNNKTNWWAYQTNR
                     AEVSKMFDKAILSYTDASTLPEAWDAMFKHFNEQQGKGSVGYTPGEKIYIKVNITNSA
                     NGMTKTQNFDRMDLTPEMALILLRHLIEEVGVAQSDIYLGDPFRNFHNLYWDMCHSVY
                     PDV"
     gene            1095..2333
                     /gene="pepT"
                     /locus_tag="PKJ43_02725"
     CDS             1095..2333
                     /gene="pepT"
                     /locus_tag="PKJ43_02725"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HNZ71503.1"
                     /translation="MKQVAERFLQYIKIDTQSDPKSGKHPSTPSQFDLANLLANELQE
                     IGLIDVTVSKEAYVMATLPSNVNKKVPVVGFIAHMDTSPDFTAKDINPQIIANYKGDD
                     LVLNKAQNIVMPVSEFPELKNYVGQTLITTDGTTLLGADDKAGIAEIITAMEFLASHP
                     EIEHGDIKIAFTPDEEIGEGADSFDVEKFAANFAYTLDGDELGVLEYENFNAASAVVT
                     VKGRSVHPGYAKNKMRNSMLVAHKFLSMLPETEIPEKTEGFEGFFHLTSISGSVEKTV
                     LEFIIRDHNRQLFEKRKELMKSIMQQLNFEYGQSAVVVEIKDQYYNMLEKIEPVKFII
                     DIAAGAMRDSGIEPKIKAIRGGTDGARLSYMGLPCPNIFSGGQNFHGRFEFISVQSME
                     KAVDVILRIVERVAAFKIPD"
     gene            2343..3407
                     /locus_tag="PKJ43_02730"
     CDS             2343..3407
                     /locus_tag="PKJ43_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71504.1"
                     /translation="MKNSTPIKIIFILCSIFFPLLVFGQKWVADTLVVNFGKSAIPNC
                     SFSIGKVVDSRLGSPDFLSIFEYKKWLYFPVDQVVVVPSPLVGEFSKKFIAGSDSVPT
                     YGVNVARFVVNKKDTNRNRNLSLLATLEVYNVSNTGDSVFCGTFYYEQILKQKKKEML
                     SLGYEQIIDDWARRFVSDIVSVDAGLTALNPDNFYHFRRGERAVNKNLYASVEMFYGF
                     NFYGLDAELWFSEPEASQIFQRNSGVFRFVNYSNSQSVALGRQVGRWNYRFSNNWLFT
                     NKVLLLLGLNRWKDHKQVAHKFEEIFLFDASMSQQINYNKLDKTGFVFGVGLVEDLHY
                     VIHRKVSLNVGLSFNCAYKF"
     gene            3409..>3863
                     /locus_tag="PKJ43_02735"
     CDS             3409..>3863
                     /locus_tag="PKJ43_02735"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNZ71505.1"
                     /translation="MSRVVIVILSMCISLSVFGQRYSVSGIISDAQTGETLVGASVLL
                     KDSQLKGTTSNENGFFTLAGILTPKVEIAVSFVGYESQLRSIDFVDENKVFIEIKLHP
                     SKVLLNEINVVEEGVDKLGDREIEISRHSLTPKVIQSIPTARNDVFKAIR"
BASE COUNT         1105 a          663 c          844 g         1251 t
ORIGIN      
        1 ttgacatcgg gataaactga atggcacata tcccaataca ggttgtggaa attgcggaac
       61 ggatcaccga gatagatatc tgattgtgct acaccaactt cttcaataag atgtctgagt
      121 aaaatcaacg ccatttctgg tgtaaggtcc atacggtcga agttttgtgt tttggtcatt
      181 ccgttggccg aattggtgat attcactttg atataaatct tttcacctgg ggtatatcca
      241 acagaacctt tgccttgttg ctcgttgaag tgtttgaaca tagcatccca ggcttctggt
      301 aatgttgatg cgtctgtgta actcaggatc gctttatcga acattttaga tacttctgct
      361 cggttggttt gataggccca ccaatttgtt ttattgttag acggtgtgaa attctcattt
      421 gtcgcatttt tgttccaaac ccaaactact ctgcctggtt tcagtccgtc ggcagttcca
      481 acaggattgt ttgcttcaaa ataactggct tcaactaagg ttgccgcctt tgattcttgg
      541 cctgttgata cattggccgc aataatcatt acaaaagttg ctgccaaaag agcagttgct
      601 gctacgtatt tcgacgcctg taactttttg tgaaacaagc gaattgcaaa ggctgagcct
      661 gcaatagaaa ggatataaac tacaaaaccc gacataaaag gtgctgcagc tttcatacat
      721 gggtaagtgg ctctcgatgg tttagggata accctaatga gaaaccaaat ggttgatgct
      781 aaccctaata caataaaact tagtctggcc caaagagcct ttccagaaat ttttttcata
      841 agtattgtgc tttatagaat attaacttta gatgcttttt ttttaataag gtttaaagtt
      901 acatttttta tacttggatt tagggacgaa aaattaaact aacaaaaatt taacgtaaaa
      961 aactgtttct tcaatacgct gctattgcat tggtctgttt tttcaattcc gcttaacata
     1021 ggcaaatatc tttttgtgtt cctgtttttt tcgggccgaa agtgttactt tggactttat
     1081 taaaggaatg tcttatgaaa caagttgccg agaggttttt acaatacata aaaatagata
     1141 ctcagtctga tcctaaaagt ggtaaacatc caagtacccc gagtcagttt gatttagcaa
     1201 atttattagc caatgagttg caagagattg gtttgattga tgttactgtg agcaaggaag
     1261 cgtatgtgat ggctacattg ccttcaaatg taaataaaaa agttccggta gttggattta
     1321 ttgctcacat ggatacaagt cctgacttta cggcaaaaga cattaatcca cagattattg
     1381 ccaattacaa aggagatgat ttagtgctga acaaggctca aaacatagta atgcccgtgt
     1441 ctgaatttcc tgagctaaaa aattatgttg gacaaaccct tatcactacc gatggcacaa
     1501 cattacttgg tgccgatgat aaagccggta ttgctgagat aattaccgcg atggagtttc
     1561 ttgcgtcgca tcccgaaatt gaacatggcg atattaagat agcctttacc cccgatgaag
     1621 agattggtga aggggctgat tcctttgatg ttgaaaagtt tgcagccaat tttgcttata
     1681 ctctcgacgg tgacgagctg ggggttcttg aatatgaaaa ctttaatgca gcttcggctg
     1741 tggttacggt taagggacga agtgttcacc cggggtatgc caaaaataaa atgaggaatt
     1801 cgatgttggt tgcccataaa tttctatcga tgttgcctga aaccgaaata cccgagaaaa
     1861 cagaaggttt tgaaggtttt tttcatctta cttcaatttc aggaagtgtt gaaaaaacgg
     1921 tgttggagtt cattatccgc gatcataacc gccagctttt cgagaagcgt aaagagctga
     1981 tgaagagtat tatgcaacag ctaaatttcg aatatggtca gtctgcggta gtagttgaga
     2041 ttaaggatca gtattataac atgctagaga aaattgagcc tgtaaaattt ataatcgata
     2101 ttgctgccgg tgccatgcgc gatagcggta ttgaacccaa aataaaagcc atccgtggag
     2161 gcaccgatgg agcaaggttg tcatatatgg gcttaccctg cccaaatatt ttttcaggag
     2221 gacaaaattt tcatggacgt tttgagttta tttcagtgca atctatggaa aaagccgttg
     2281 atgtgatctt gagaattgtt gagcgtgtag cggcatttaa aattcctgat taattattga
     2341 aaatgaaaaa ctcaactccc ataaaaatta tctttatttt atgttcgatt tttttcccat
     2401 tgctggtttt cggtcagaaa tgggtggccg atacgttggt cgttaatttt ggaaagtcag
     2461 ctattcctaa ttgttctttc tccataggaa aagtcgtaga cagcagactt ggttcgcccg
     2521 attttctttc aatatttgag tacaaaaaat ggctttattt tcctgttgac caggttgttg
     2581 ttgtgccaag cccattggtg ggggaatttt ctaagaagtt tattgctggc agtgattctg
     2641 tgccgacata tggggtaaat gttgctcgct ttgttgtaaa taaaaaagat acaaatagga
     2701 atcgtaatct ttcactgctt gctactctcg aggtatacaa tgtttcgaac acgggtgact
     2761 ctgttttttg cggtactttt tattacgagc aaatacttaa gcaaaagaag aaggagatgt
     2821 tatctctcgg atatgagcaa attatcgacg attgggcaag aaggtttgtg agtgatattg
     2881 tttctgtcga tgctgggttg accgctctaa accctgataa tttttatcat ttcaggagag
     2941 gggaaagagc ggtcaataaa aacttgtacg cttcagtaga gatgttttat ggtttcaatt
     3001 tttatggtct tgatgctgag ttgtggtttt cggaacccga agcatcccaa attttccaac
     3061 ggaactcagg cgtttttagg tttgttaact attctaattc acaatcagtt gcattaggcc
     3121 gacaggtggg gcgctggaat tatcgttttt ccaacaactg gctttttacc aataaagtac
     3181 tgttgttact tggcttaaac cgatggaaag accacaaaca ggtagctcat aagttcgagg
     3241 agattttttt gtttgatgct tctatgtcgc agcaaatcaa ttacaataag cttgataaaa
     3301 ccggatttgt ttttggtgtt ggtcttgttg aagatttgca ttatgttatt catcgcaaag
     3361 taagcttgaa tgttgggctt tcgtttaatt gcgcatataa attttagtat gtcgagagtt
     3421 gtaatagtaa tactatccat gtgtatatca ctttccgttt ttgggcagcg gtattctgtt
     3481 tcgggcatta tttccgatgc tcaaacaggt gaaacactag ttggggcttc tgttttgctg
     3541 aaagatagtc agctgaaagg aacaacttca aacgaaaacg gtttttttac attggccggg
     3601 attttgactc ccaaggttga aattgcagtt tcttttgttg gatatgaatc gcagctcaga
     3661 tcgatcgatt ttgttgatga aaataaagtg ttcatcgaaa taaaacttca cccgtcaaag
     3721 gtattgttaa acgaaataaa tgtcgttgaa gagggggttg acaaattggg cgatcgggaa
     3781 attgagatca gcagacattc gctcactccc aaagtaatac aaagcattcc taccgctagg
     3841 aatgatgtgt ttaaggcaat tcg
//
LOCUS       DAOPUW010000105         3856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_23900_length_3856_cov_3.114180, whole genome shotgun sequence.
ACCESSION   DAOPUW010000105 DAOPUW010000000
VERSION     DAOPUW010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23900_length_3856_cov_3.114180"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(479..2917)
                     /locus_tag="PKJ43_02740"
     CDS             complement(479..2917)
                     /locus_tag="PKJ43_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010425064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNZ71506.1"
                     /translation="MKFGYFDDKNREYVITNPQTPWPWINYLGNEDFFSLISNTAGGY
                     TFYKDAKFRRLTRYRYNNVPMDSGGRYFYIKDQETVWSPGWKPCKTPLDSYECRHGMN
                     YTILNSSKNGVSAKVTFFVPLKTWAEVQKLTLTNNSSEVKKLKLFSFTEWCLWNAATD
                     MENFQRNFSTGEVEIEGSVLYHKTEYKERRDHYAYYSVNTPIDGYDTDRENFIGLYNG
                     FENPQVVEEGKPRMTVAHGWSPIASHYIEVELKPGESKDFIFLLGYVENAQDEKWESK
                     GVINKTKAKATIANFDTVEKVDAAFAELRAYWYNLLSIYSVNSGDDKLDRMVNIWNQY
                     QCMITFCFSRSASYFESGIGRGMGFRDSNQDLLGFVHQIPERARERIIDIASTQFQDG
                     GCYHQYQPLTKKGNNDIGGGFNDDPMWLILGTISYIKESGDFSILDEMVPFDNDMSVA
                     RTLFDHLTVSFDHVVNNLGPHGLPLIGRADWNDCLNLNCFSNDPNESFQTTENKTEGS
                     KAESVMIAGLFVIYGQDYVDLCKKLGKTDEAARAQAHVDAMKVAVKEHGWDGEWFIRA
                     YDYYGNKIGSNECEEGKIFIESNGWCTMAGIGKEEGLCEKALDSVKERMDTPFGIVLN
                     NPAFTKYYIEYGEISSYPAGYKENAGIFCHNNPWIMIGETVIGRGDQAWEYYKKICPS
                     YLEEISDLHKTEPYVYAQMVAGKDAFKPGEAKNSWLTGTASWNFYTISQFILGIQPDY
                     DGIKVDPCIPAAWKGFEVTRKFRGATYQIKVENPNSVCKGVKKVTVDGKVITGNILPV
                     FESGTTHSVTVELG"
     gene            complement(2953..>3856)
                     /locus_tag="PKJ43_02745"
     CDS             complement(2953..>3856)
                     /locus_tag="PKJ43_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ71507.1"
                     /translation="NKPYSFIAQGEANLTASTVRLINQKTGYSYAIYFLSFFLALFLI
                     ITFYTTRERVSPPKNQDSNLIRDLKDLIHNRPWVVMLLIGLLFNIYNSTKQGILLYYF
                     THYMHSPLLSATYMIGLMIASIAGAMITSPLGRKFGKKNLFIGALIFSGIVNAMMALC
                     GPNDKVAIFTIGMISEFGAAIFPTLFFAMLGDAADYSEYKNGRRATGLVYSAGSFATK
                     FGGGIAGALIGFVLTAFHYNGQDSSTIQGAIPGIVMLMSWIPAIITLIAAAVMTLYPL
                     SQKKMDEITIELNNRRAAELAQNN"
BASE COUNT         1011 a          817 c          856 g         1172 t
ORIGIN      
        1 aaagaggtga ttgatgtttc caagcttgag aaaggatatt acattcttca tatccgaaca
       61 aaacaggcac tttatcagga acacattctt ataaaataga aatgatacct attttacctt
      121 tggggaaaga aaaaggctgt tgtgtttgcg atagactttt tcttagaata tctgtaatta
      181 ccactcgaac agcggaaaat ctttgaatct ctctaactcc atactaagtt ataccagatg
      241 caattctaaa tcagtccgaa cctatccatg tataatgccg atgaatagaa atgttcagta
      301 caatcgagtg gacgaagatt ggactgttat atttcttcaa tcggtattag accccagttt
      361 cctaattttt ttaactcatg gcaacaggta ttaagtggat tattatgcaa tgttgaaggg
      421 taaaaagaaa tccgcagtgg gttgctgcgg atctcaaaaa aaaagtaggt ttatttaatt
      481 agcccaattc tacagttacc gagtgggttg tcccggactc gaatacgggc aggatgtttc
      541 ctgtaattac ttttccgtca acagtgactt tctttacccc tttgcacaca ctgttggggt
      601 tttcaacctt gatttggtag gtggcaccac ggaatttgcg ggttacttca aaacctttcc
      661 aggcggctgg aatacaagga tcgactttga ttccatcgta gtcgggttga attccaagaa
      721 taaactggct gatggtgtag aaattccacg atgcagttcc tgtcagccat gaatttttgg
      781 cttcgcctgg tttgaaagcg tccttaccgg ccaccatttg cgcatataca tacggttcgg
      841 ttttgtgtag gtcgctgatt tcttcaaggt atgaagggca tattttctta tagtattccc
      901 atgcctgatc gccgcgacca ataacggttt cgccaatcat gatccatggg ttgttgtgac
      961 agaaaatacc ggcgttttct ttataccctg ctgggtaact agagatttcg ccatattcaa
     1021 tatagtattt tgtgaaagcc ggattgttga gcacaattcc gaaaggtgta tccatacgct
     1081 ctttcactga gtcgagggct ttttcgcaca aaccttcttc tttgccaatg cccgccattg
     1141 tacaccatcc gttcgattcg atgaagattt taccttcctc gcattcgttg ctgccaattt
     1201 tgttgccata gtaatcatag gcgcggataa accattcgcc atcccagcca tgttctttta
     1261 cggcaacttt cattgcatct acatgagctt gtgcgcgtgc agcttcgtcg gttttcccga
     1321 gttttttgca aaggtcaaca tagtcttgtc cgtaaatcac gaacaaaccg gctatcatta
     1381 ccgattcagc tttcgagcct tcggttttgt tttcggttgt ctggaatgat tcgttaggat
     1441 cgttcgagaa acagttcagg ttcaaacagt cgttccagtc ggcacggcca atcaacggca
     1501 atccgtgtgg gccgaggttg ttcaccacgt ggtcgaacga aacggtaagg tgatcgaaca
     1561 aggttcgggc tacgctcatg tcgttgtcga aaggcaccat ttcatcgagg atcgagaagt
     1621 cgcccgattc cttgatgtag ctgatggttc caagaataag ccacatgggg tcgtcgttga
     1681 aaccgccgcc aatatcgttg ttcccttttt ttgtcagagg ttgatattga tggtagcaac
     1741 caccatcttg gaactgtgtt gatgcaatgt cgatgatgcg ttcgcgggca cgctcgggta
     1801 tctggtgaac gaagccgagc aaatcctggt tggaatcgcg gaaacccatt ccacggccaa
     1861 tgccgctttc gaaatatgaa gctgagcgcg agaagcagaa ggtgatcata cattgatatt
     1921 ggttccagat gttcaccatg cggtcgagct tatcatcgcc cgaattcacg ctgtaaatgc
     1981 ttaagaggtt gtaccaatat gcacgtaatt cggcaaaagc ggcatccact ttttcaactg
     2041 tgtcgaagtt tgcaatggtt gcttttgctt tggttttatt gattacgcct ttcgattccc
     2101 atttttcgtc ctgggcattt tcgacatatc ccaacaagaa gatgaaatct ttcgattcac
     2161 cgggtttcaa ttccacttcg atatagtgcg acgcaatggg cgaccatccg tgggcaactg
     2221 tcatgcgggg ttttccttct tcaaccactt gtgggttttc gaagccatta taaaggccaa
     2281 tgaaattttc gcggtcggtg tcgtacccat caatcggggt atttactgaa tagtatgcgt
     2341 aatggtcgcg gcgttctttg tactcggttt tatgataaag cactgaacct tcaatttcta
     2401 cttcgccagt agagaagtta cgctggaaat tttccatgtc ggtagcagca ttccacaagc
     2461 accattcggt aaacgaaaag agtttcagtt ttttcacctc cgaagagttg ttggtcagcg
     2521 taagtttctg tacttcggcc caggttttca gtggtacaaa gaacgttact ttggccgaca
     2581 caccgttttt cgaactattc agaatggtgt aattcatccc gtggcggcat tcatagctgt
     2641 cgagcggggt tttgcaaggt ttccatcccg gcgaccaaac tgtttcctga tcttttatat
     2701 agaaatagcg gccaccgctg tccatgggta cgttgttgta acggtaacgg gtaaggcggc
     2761 ggaatttagc atccttgtaa aaggtgtatc caccggctgt atttgatatc aaagaaaaga
     2821 aatcttcgtt accaaggtag ttaatccatg gccatggggt ttgcggattt gtaattacat
     2881 attcgcggtt tttgtcatca aaatatccga acttcatctc tcttaatgtt attcctgtta
     2941 attatatagt aatcaattat tttgtgccaa ttcggctgct ctcctgttat ttagttcgat
     3001 ggttatttcg tccattttct tttggctaag tggataaagg gtcattacag ccgctgctat
     3061 aagtgtaatt attgcaggga tccagctcat caacatgact atgccgggga ttgctccctg
     3121 aattgttgat gaatcctgcc cgttatagtg aaatgctgtc agcacaaatc caatgagtgc
     3181 acctgcaata ccaccaccaa attttgtggc aaacgatccg gctgaataaa ccaatccagt
     3241 tgcccgacga ccgtttttat attcagaata gtcggctgca tcgcccagca tagcaaagaa
     3301 tagggttggg aaaatggctg ctccaaattc tgaaatcatc ccaattgtaa aaatagccac
     3361 tttgtcgttt ggtccgcata aggccatcat tgcatttacg attcctgaaa aaattaatgc
     3421 gccaataaat aaattctttt ttccaaattt ccggcctaac ggagaggtta tcatggcacc
     3481 ggcaattgag gcaatcatca atccaatcat gtatgtggct gacaatagcg gactgtgcat
     3541 gtaatgtgta aagtaataca gcagaatgcc ttgttttgtc gaattataaa tattgaaaag
     3601 aagcccgatc aataacataa caacccacgg gcggttgtgt atcaagtctt taaggtcgcg
     3661 gatcaggttt gaatcctgat tctttggagg ggaaaccctt tccctggtag tatagaaagt
     3721 aattattaga aacagggcca aaaagaacga aagaaaataa atggcatagc tatagccagt
     3781 tttttggtta attaacctga cagtcgatgc tgttaaattt gcttcaccct gagcaataaa
     3841 tgagtagggt ttgttg
//
LOCUS       DAOPUW010000106         3848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_23991_length_3848_cov_3.078302, whole genome shotgun sequence.
ACCESSION   DAOPUW010000106 DAOPUW010000000
VERSION     DAOPUW010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23991_length_3848_cov_3.078302"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PKJ43_02750"
     CDS             <1..341
                     /locus_tag="PKJ43_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71508.1"
                     /translation="GKFLVVSSQGANARSAFFYMKTKGEMEESVKKTGNRSIYIIRPS
                     LILGKRQETRIAEKIGIMLYSLFAPLMIGSLRKSRAVSATKIARCMIAMAEKDDAGIF
                     TIASETIQQY"
     gene            complement(386..2818)
                     /locus_tag="PKJ43_02755"
     CDS             complement(386..2818)
                     /locus_tag="PKJ43_02755"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ71509.1"
                     /translation="MKRFYFFFIIVMLSGILAANAQTSNATIFGKVTDENGLPVDMVN
                     VSLKNYPFGTTTNRKGEYLFRIPSGREVAVVFSMIGYNMEERKLSLASEATFELNVVM
                     SSSNQEIDEIVIQEQRQTSGNIVRINPKSVSSLPDVGMGSVEGVLRTLPGVSSSNELS
                     NQYSVRGGNFDENLVYVNGIEVYRPTLIRAGQQEGLSFVNSDMVSSIQFSAGGFDASY
                     GDKMSSVLDIKYNRPTQFKANVMASLMGARVHVENISADKKFTYNMGARYKSFKLLLG
                     SLAEKGAYDPSFIDFQGYFTYSPSPKFEIGFLGTASSNSYRFVPEKLEQTFGTFDNQL
                     KLSIDFDGQEVDRFYTYTGALNFLYKPTPAINLNLALAAFSSDEQETYDIIGSYRFNE
                     VQNEGTDQRDDSTILLGLGYYHEHGRNFLKANVLSVDHSGSFKSGGNVLSWGMNAKQE
                     IISDKMNEWQYRDSAGYSLPYSHKEVKLYYAENTEYEHTKNRYSAYLQDSYSTPWGNG
                     TLMITGGVRANYWDFTHKVFVSPRFSCSYKLDGTYDIIYRLAAGSYYQPAFYRELKNL
                     YGEVADSIQTPHSLQVVAGVDYSFIAWERPFKLTAESYYKSLKNLIPYQYENVRIRYL
                     SDQISDGFAYGLDVKVNGEFVSGTQSWVSASLMKTMEDIDGDVDSEGNQVGYIRRPND
                     QRFKFSMFFQDYLPGLPYYQMNLTGHFITGVPYGMPRSPRYLQRSQIPAYRRVDIGFL
                     RLLVSNGDNLTSWRFLDRFKECSVGIEVYNLLDIRNVSSYSFIADFQNIYYAVENRLT
                     GRMLNFTVSASF"
     gene            2980..3621
                     /locus_tag="PKJ43_02760"
     CDS             2980..3621
                     /locus_tag="PKJ43_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNZ71510.1"
                     /translation="MFISLLLKGLLIGFLVSMPIGPIGVMVIQRTANRNFKSGFFSGL
                     GVAFTDSNWAILAGFSVSYIITFLQTYQLYIQIIGAVLLIALGLNIFLSHPVTEWKKM
                     RRKGTSPMEMFLTSVGISLSNPLPILTYIAIFASAHVVFDITNLWEPAFFVSGFFIGA
                     TLWWTTLAFTINHFRHKFNLRILWWFNKVSGSLIMLLVVATSIFVMVKGNPLL"
BASE COUNT         1173 a          848 c          762 g         1065 t
ORIGIN      
        1 gtggcaaatt tctggtagta agctcacaag gtgccaatgc ccgctcagca ttcttctaca
       61 tgaaaaccaa aggggaaatg gaagagtcgg tgaaaaaaac aggtaaccgc agcatttata
      121 tcatacgccc gtcgctaatt ttggggaaaa gacaagaaac cagaatagcc gaaaaaattg
      181 ggatcatgct gtattccctt ttcgctccgc tgatgattgg gagccttcga aaatcaagag
      241 ctgtaagcgc tacaaaaata gcccgttgca tgattgcgat ggcagaaaaa gacgatgccg
      301 gtatttttac catagcctcg gagaccattc agcaatacta atcgaaggcg ctgattctag
      361 atgtaatacc gaattgctgg aatgcttaaa aagaagcaga tacagtaaaa ttcaacatcc
      421 tgccagtaag cctgttttcc actgcataat agatattctg gaaatcggct ataaaagaat
      481 acgaagatac attcctgata tcgagtaaat tatacacttc aatcccgaca ctacactctt
      541 taaacctatc gagaaaacgc cacgaggtaa ggttatcgcc attactaacc aggagtctta
      601 gaaacccgat atcgaccctg cgataagccg gtatttgact cctctgcaaa tagcgtgggc
      661 tgcggggcat tccataaggc acaccggtaa taaaatgtcc tgtcaggttc atctgataat
      721 agggcaaacc gggtaaataa tcctgaaaaa acatgctgaa tttaaagcgc tggtcattgg
      781 gccttctaat ataccccact tggtttcctt cgctatctac atcaccatca atatcttcca
      841 tggttttcat cagcgaagcg ctaacccacg attgggttcc gctcacgaac tcgccattca
      901 ccttaacgtc gaggccatag gcaaaaccat cagatatttg gtcgctaaga taacggatac
      961 gcacgttttc gtactgataa ggaataagat tcttcagcga tttataatac gattctgcag
     1021 taagtttaaa aggcctttcc catgcaataa acgagtagtc gaccccagca acaacctgca
     1081 aggaatgagg ggtttgtatt gaatcagcca cttcaccata aagatttttc aactcgcggt
     1141 aaaatgccgg ttgataatac gagcctgcag ccaggcggta aataatatca tacgttccat
     1201 cgagtttata agagcacgaa aaacgcgggc taacaaatac tttatgggta aaatcccaat
     1261 aatttgcccg tacccctccg gtaatcatta atgtaccatt tccccaagga gttgaataag
     1321 aatcctgtaa atatgctgaa tacctgtttt ttgtatgttc atattcagta ttttccgcat
     1381 aataaagttt aacttctttg tgggaataag gaagcgaata tcctgcagaa tcgcgatatt
     1441 gccattcatt cattttgtct gagataatct cctgcttagc attcattccc cacgacagca
     1501 cgtttccccc actcttgaac gacccagagt gatcaacgct taacacattt gccttgagaa
     1561 aattacgacc atgctcatga taataaccta acccaagtag tatggtactg tcatctctct
     1621 gatctgtccc ttcattctgc acttcgttaa agcgatagct cccaattata tcgtaggtct
     1681 cttgttcgtc ggaagaaaaa gcggcgaggg caagattgag gtttattgca ggcgttggtt
     1741 tatatagaaa atttaaggct ccggtatagg tataaaaccg gtcaacctcc tgtccgtcga
     1801 aatcaataga aagtttcaac tggttatcaa aagtgccaaa agtctgttcc agtttttcgg
     1861 gtacaaaacg gtagctgttt gacgaagctg tacccaagaa acctatttca aattttggcg
     1921 aaggagaata tgtaaaataa ccctgaaaat cgataaacga gggatcatat gcccctttct
     1981 cggcgaggga acccaacaac aatttgaacg atttataccg tgcgcccatg ttataggtaa
     2041 acttcttgtc agcgcttatg ttctcgacat gtactcttgc ccccattaaa ctagccatta
     2101 cattggcttt aaactgggta gggcgattat atttaatgtc caaaaccgaa gacattttgt
     2161 cgccatacga agcatcgaag cctccggctg aaaattgaat ggatgaaacc atatctgaat
     2221 tgacgaagct taagccctct tgctgtccgg cacggatcag ggtaggccta tacacttcta
     2281 tgccatttac atacaccaga ttttcatcga agttgccccc acgcaccgag tactggttgc
     2341 tgagctcgtt gctcgagcta accccgggaa gtgtccgcaa aacgccctca acgctaccca
     2401 tccccacatc gggtaaagag ctgactgact ttggattaat acggacaatg tttcccgatg
     2461 tctgacgttg ctcctggatc acaatctcat cgatttcctg attggaagaa ctcataacca
     2521 cgttcaattc gaacgtagct tcagaggcca gcgaaagttt tcgttcttcc atgttgtacc
     2581 caatcataga aaaaacaaca gccacttcgc gccccgaagg aatgcgaaaa agatattcac
     2641 ccttacgatt ggttgtagta ccaaaaggat aatttttcag gcttacattg accatatcaa
     2701 caggcaatcc gttctcatca gttaccttgc caaatatggt agcgttgcta gtttgggcat
     2761 tcgcagccaa tatgcctgaa agcattacta taataaagaa aaaataaaac cgtttcattg
     2821 accgcaaaga taatattcat tgacaaaaca agaacaaaac gtgcaaaatc gaaattaatt
     2881 gcagaaaaaa caggaaagat agaattttga ataatttaac tttcatggtc aaaagaaatg
     2941 gggtgaaggc tgtatattta cagccgcaaa aaagaagaaa tgtttatcag tctgttatta
     3001 aaaggattat taatcgggtt tttggtctcg atgcccatag gccccatcgg ggttatggtg
     3061 attcagcgca cggccaaccg taatttcaag tcaggattct tttcggggct tggtgttgct
     3121 tttaccgatt caaactgggc aatactggcc gggttcagtg tttcgtatat cattactttt
     3181 cttcagactt atcaattata tattcaaatt attggtgcag tactgcttat tgcccttggg
     3241 ttgaatatct ttttgtcaca tcccgtaacc gaatggaaaa agatgagacg aaaaggtaca
     3301 agccctatgg aaatgttcct gaccagtgtg ggtatctcgc tttcgaaccc cttacctatt
     3361 ctgacataca ttgctatttt tgccagtgcc catgttgttt ttgatataac caacctatgg
     3421 gaacccgctt ttttcgtgtc cggctttttt attggggcaa ccctttggtg gacaaccctg
     3481 gcatttacca tcaaccactt caggcataaa ttcaacttac ggatattatg gtggttcaat
     3541 aaagtatcgg gatcgctgat tatgctgctt gttgtggcaa cttcaatatt tgtcatggta
     3601 aaaggcaatc cactccttta gcaagatcac caccaacaaa caaaactgaa ctgttgcaac
     3661 cttgcacatt agttttaatc ggtattacag catttttcac gattatttcg tacttttcta
     3721 tcccaaaaaa acgaagcaaa accatggata ctgagaaaac aaagaaaaat ttatacgaaa
     3781 caattttcga agcagatacc cgaaaaggaa agctatttga cgaagtgctg atggtgttaa
     3841 tcttgctg
//
LOCUS       DAOPUW010000107         3842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_24041_length_3842_cov_2.656456, whole genome shotgun sequence.
ACCESSION   DAOPUW010000107 DAOPUW010000000
VERSION     DAOPUW010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24041_length_3842_cov_2.656456"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="PKJ43_02765"
     CDS             complement(<1..891)
                     /locus_tag="PKJ43_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71511.1"
                     /translation="MKRSFAQLFISFLILLTFSGCNSFRSAMKTYEIGEYYNAIKELQ
                     DASRKEKDPALRMEIDHKIALSYWRIGDYKKAEPRFKNLITKKFSADSTLILKYAHSL
                     RFNGKYPDAITNYKQYLDSFPENKEALNGIKSCELTPIWEEKPTRFIVTQEKPLTSRF
                     ADYSPVYVGGLDNSLIFTSTRDGATGRKKSRITGQRNGDLWKSYFDIQKQKWEKPELI
                     DNEELINTPNDEGAPTLSADGSLMFFTRCRFDKAKAMGAEIYETQKSKDTWSEPTSVE
                     ILGDSLVVAHPSLSKDGLTLY"
     gene            complement(996..3287)
                     /locus_tag="PKJ43_02770"
     CDS             complement(996..3287)
                     /locus_tag="PKJ43_02770"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HNZ71512.1"
                     /translation="MRHFISIAFLCMCISSFGGHTIWLDTPAEEWEVALPIGNGRLGG
                     MIFGGVEYERIQLNEESLWTGSRYQFTDKPEAWKQLPEIRKMLFNGEYSRAQEITENE
                     FMGINNWNMYQTLGDLHISFPTAKKTENYRRELSLKQALAKVTYTADDVEYTREYFSS
                     AADQVMVMKISSTRKNGLSLGVRLSRPKDASINSDNKHITMAGQVTAGGIDMQGINPG
                     VKYFAILEAKTDDGEIVESGDSLLINKATTVYLFFTAATNYWGDDPESKSLSNLKNAT
                     QKTYDELKARHIADYQQYYQRVNLDLGGKEKSLLPTNKRLALIQEGEPDNDLMATYFN
                     FGRYLLISSSREGDLAANLQGIWADGLIPPWSADYHININIQMNYWPAEVTNLSECHL
                     PFIALTDSLRRHGRETARNMYNSRGFVAHFDTDAWYWTTAVGKAEWGMWPLGAAWCCS
                     HIWQHYLFTQDIDFLEKNYEILKEASMFFIDYLTIDPKTGYLVTGPSSSPENKFETPD
                     GKISNITMGPTMDMSITRELLKNTIHASKVLKKDRKFRKKLQSVLSQLSPLKIGSDGR
                     IMEWTEEFKEPEPGHRHISHLYGLHPGNEITPAETPELAAAARKTIDYRLSHGGGHTG
                     WSRAWIINFFARLGDGEKAYENMMALLQKSTKPNLFDTHPPFQIDGNFGATAGIAEML
                     LQSHANAIDLLPALPSAWSDGKVSGLKARGAFEVDMEWKNGELLSATIRSLKGNPVKV
                     RYNGKEKIFKGKAGEIFSIGSTW"
     gene            3501..>3842
                     /locus_tag="PKJ43_02775"
     CDS             3501..>3842
                     /locus_tag="PKJ43_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNZ71513.1"
                     /translation="MNDEKEVTIYDIARELKLSASTVSRALKGNPIINYKTRQKVAEC
                     SEKLGYRTNAFASNLRRKKTNTIGVIVPRLDSHFLSSCLAGMEEVASTNGYNLIIAQS
                     HESSKKEAENAV"
BASE COUNT          942 a          822 c          871 g         1207 t
ORIGIN      
        1 ataaagggta agcccgtctt tcgaaagaga tggatgggca acaacaagac tatccccaag
       61 gatctcaaca ctagtcggtt ccgaccaagt atccttgctt ttttgtgttt cgtatatttc
      121 tgcccccatg gcttttgcct tgtcgaatcg gcagcgggtg aaaaacatca gacttccatc
      181 ggccgaaagg gttggtgctc cttcgtcgtt gggagtatta ataagttctt cgttgtcgat
      241 tagctctggt ttctcccatt tttgtttctg aatatcgaaa tatgatttcc acaggtcgcc
      301 gtttcgttgc cctgtaattc ggcttttttt ccttcctgta gctccatcgc gtgtcgatgt
      361 gaaaatgagg ctattatcga ggcctccaac atataccgga gagtaatcgg caaaacggga
      421 ggtgaggggt ttttcttggg ttacaataaa acgggttggc ttttcttccc aaattggggt
      481 taactcgcat gattttatcc cgttaagggc ttctttgttt tcgggaaagc tatccaggta
      541 ttgtttgtaa ttcgtaatgg catcgggata ctttccattg aaacgcagcg aatgggcata
      601 ttttaatatc aaggttgaat cggcgctgaa cttttttgta atgaggttct taaaccgagg
      661 ttctgctttt ttgtagtcgc caattcgcca gtacgaaagg gcaattttgt gatcaatttc
      721 catcctcaat gccgggtctt tttctttccg cgaggcatct tgcaattctt ttatagcgtt
      781 gtaatattca ccaatttcat acgtcttcat ggcggagcgg aacgagttgc atcccgaaaa
      841 tgtcagaagg attaaaaatg agataaatag ttgggcaaaa gatctcttca tacaattagt
      901 ttcaaaaacg ggttaaaaat aacgattttt cgttgaaagc cccgaagttc tgttttcgtt
      961 acctttatta acgttctttt tattgaaatg gtatgttacc aagtgcttcc aatgctgaaa
     1021 atttcgcccg cttttccctt aaatattttc tcttttccgt tgtatcttac cttaaccggg
     1081 tttcctttta aagacctgat agttgccgaa agcagttccc cgtttttcca ttccatgtca
     1141 acctcgaatg ccccgcgggc tttaagcccc gagacttttc cgtcggacca ggccgatggt
     1201 agtgctggca gcaggtcaat ggcattggca tgactttgaa gcagcatttc tgcaatgcct
     1261 gctgttgctc caaaattgcc gtcgatttga aagggtgggt gggtatcgaa caaatttggt
     1321 tttgttgatt tctggagcag ggccatcatg ttttcgtacg ctttttctcc atcgccaagc
     1381 cgggcaaaaa agttaatgat ccaggcgcgg ctccatccgg tatgccctcc gccgtgcgat
     1441 aaacggtagt caattgtttt tcgggctgct gcagccagtt cgggggtttc tgccggggtt
     1501 atctcattac caggatgtaa tccatacagg tgtgatatgt ggcggtgacc gggttcgggc
     1561 tctttaaatt cttctgtcca ttccattatc cgtccgtcgc ttccaatttt caatgggctc
     1621 aactggctta acaccgattg aagttttttc ctgaattttc tatccttttt taggactttg
     1681 gaggcatgga tggtgttttt cagtagttcg cgggttatcg acatgtccat ggtgggtccc
     1741 atggttatgt ttgaaatttt accatcgggg gtttcgaatt tgttttcggg tgaactcgac
     1801 gggccggtaa ccaggtaccc ggttttggga tcgatggtta ggtagtcgat aaaaaacatt
     1861 gaagcttctt tcagtatttc atagttcttt tcaaggaaat cgatgtcttg tgtgaaaaga
     1921 taatgttgcc agatatgtga gcagcaccag gcggcaccca agggccacat tccccattct
     1981 gccttgccaa ccgctgttgt ccagtaccat gcatcggtgt caaagtgggc aacaaaaccc
     2041 cggctgttat acatatttcg ggcggtttcg cgtccatgcc ttcgcaagct gtcggttagg
     2101 gcaataaatg gcaggtggca ttctgaaaga ttggtaacct cggcaggcca atagttcatc
     2161 tgtatattta tgttgatatg gtaatcggca ctccagggag gaatcaaccc gtcggcccag
     2221 ataccctgga ggtttgcagc aaggtctcct tcacgcgaac tgctgatcaa cagatatctt
     2281 ccaaagttaa aatatgtagc catcaggtcg ttatctggtt cgccttcttg tattagtgca
     2341 aggcgtttgt tggttggaag tagtgatttt tcctttccac ccaagtcgag gttaactctt
     2401 tgatagtatt gctgataatc ggcaatatgt ctggctttta attcatcgta tgtcttttgt
     2461 gttgcatttt tgaggttgct caggctcttg ctttccggat catctcccca ataatttgtg
     2521 gctgctgtaa agaaaaggta tacagtagtt gctttgttta taagcaaact gtcgcccgat
     2581 tcaacaattt ctccatcatc ggtttttgct tcaagtatgg caaagtattt tacgccgggg
     2641 tttatccctt gcatatcaat gcctccggca gtaacttggc cagccatggt aatgtgctta
     2701 ttgtcgctat taattgaagc atctttaggg cggcttagtc taacccccaa cgagaggccg
     2761 ttcttccggg ttgaacttat tttcattacc ataacctggt cggctgcact cgagaagtat
     2821 tcgcgtgtat attctacgtc atcagcagtg tatgttactt ttgccaacgc ttgtttcaga
     2881 gaaagttcgc ggcggtagtt ttctgttttt tttgcagtag ggaagctaat atgcaaatcg
     2941 ccaagggttt ggtacatgtt ccagttgttt atacccataa actcattttc agtgatttcc
     3001 tgagcacggg aatattcgcc gttgaaaagc attttacgta tttcgggcaa ttgtttccag
     3061 gcctcgggtt tatcggtaaa ttggtagcga ctaccggtcc aaagcgattc ttcgttaagc
     3121 tgaatgcgct catattctac gcctccgaaa atcatcccac ccagacgtcc gttcccaatt
     3181 ggaagggcaa cctcccattc ttcggcaggt gtgtcaagcc aaatagtatg tcccccgaat
     3241 gacgaaatac acatgcataa aaatgcaata ctgatgaaat gcctcatgtc gattattttt
     3301 ctcaaaagta accaatctac agaattcggt attgacaaaa atgctacaga tattggctaa
     3361 aatgtccaat acccccgaca aatttttatc gtcatttttt acgttaaaaa aatatagtgt
     3421 ataaaaattg caatcggttg caatttgtaa tatccaatta gctacctttg tcgttcaata
     3481 gatttttggg ggaaagaagg atgaacgacg agaaagaagt taccatatac gacattgccc
     3541 gggaattgaa actttcggcc tcaaccgtta gccgtgcgct gaagggaaat ccaataatca
     3601 attacaaaac acggcaaaaa gtggcagaat gttccgaaaa attaggctat cgcactaatg
     3661 cttttgccag taatttgcgt cggaaaaaaa ccaatacgat cggggtaatt gttccgcggc
     3721 tcgatagtca ttttttgtcg tcatgtttgg caggtatgga agaagtagcc agcaccaacg
     3781 ggtataacct catcattgcc cagtcgcacg aatcgtcgaa aaaagaagct gaaaatgcag
     3841 tg
//
LOCUS       DAOPUW010000108         3815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_24256_length_3815_cov_3.966223, whole genome shotgun sequence.
ACCESSION   DAOPUW010000108 DAOPUW010000000
VERSION     DAOPUW010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24256_length_3815_cov_3.966223"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PKJ43_02780"
     CDS             complement(<1..897)
                     /locus_tag="PKJ43_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNZ71514.1"
                     /translation="MAKRKFLWSILVLLIIFFSCKDDQDKYARPEWLAGKVYSQIEDV
                     DELSEFAKCIKIVGYDSVINVSGSYTVFAPTNDAIKQYLQKNNYSSVDDIPRAKLLEI
                     VKYHIVQNPWSRDQLRSLDVNGWIDPEDEENDEPRGFKRQTLLLRDNFKCGVKVLDEE
                     KKMLMIIDSSTTTWNRRVITDSRKFAPIFYAEYFGIYNLQLSDYAFYFDRQFDNADDM
                     YYVDAKLLGQEIFAENGFIHRIDRVVEPLLNANEILTSTTDEYDYSKFLNLVNQFPSL
                     NWNSDKTYDQEGADLGLAVDSLF"
     gene            complement(1139..2566)
                     /locus_tag="PKJ43_02785"
     CDS             complement(1139..2566)
                     /locus_tag="PKJ43_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ71515.1"
                     /translation="MMDFSKHIDSVVFKAVAKQAQELGVEAYVVGGFVRDLVLGRPSK
                     DIDFVTTGDGIELASKVAHALNPRIKVNYFKNFGTAQFNYQGLDFEFVGARKESYSRD
                     SRKPVVERGTLEDDLNRRDFTINAMAISLNGNSFGQLIDPFNGLHHIDIKLIKTPLDP
                     EITFDDDPLRMMRAIRFSAQLSFSIEEETFNAIAKYSERISIVSKERIVDELNKIMLS
                     VKPSVGFKLMDKAGLLAYILPELSRMKGVEVKNGIGHKDNFYHTLEVLDQLSKKSDDL
                     WLRWSALLHDIAKPLTKKFVEGQGWTFYGHNYLGAKIIPGIFKRLKLPLNEKMKFVQK
                     MVGLHMRPIVLSEEEVTDSAVRRLLFEAGDDIDLLMTLCEADITSKNEEKVNTFLNNF
                     QLVRSKLKDIEERDAIRNFQPPVSGETIMSTFGIGPGREVGIIKNTIKEAILDGEIHN
                     NFEEAFQLMLKQGKELGLLPKNQKG"
     gene            2687..>2930
                     /locus_tag="PKJ43_02790"
     CDS             2687..>2930
                     /locus_tag="PKJ43_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNZ71516.1"
                     /translation="MLEHLTIDTFKNKVFNFEENKDWKFEGDAPCLIDFYADWCGPCK
                     MIAPVLEQLQQEYGNKLNIYKVNTEDERELAAMFGIQ"
     assembly_gap    2931..2940
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2941..3064
                     /locus_tag="PKJ43_02795"
     CDS             <2941..3064
                     /locus_tag="PKJ43_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HNZ71517.1"
                     /translation="QSIPSLLFVPKEGKPQMAMGALPKETFEQAFKEVLGVTKD"
     gene            complement(3134..>3815)
                     /locus_tag="PKJ43_02800"
     CDS             complement(3134..>3815)
                     /locus_tag="PKJ43_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNZ71518.1"
                     /translation="NVGNEHLLKMLPEGVEKITYSQAGNNAMLVGEVATDGLLLVAKV
                     LFPKGWLYIKTNLTGAYNLENVLAACCVGSYFNVDPLLIQKQIENYVPSNNRSQVMKL
                     NSNVIIVDCYNANPSSMEVSVRNFMQMKSQSKTMILGDMLELGDESETEHRKIVELVL
                     SSGIDDVYWIGRNFSQVAPDSAKKFDRVDDLLPVLGKMNYQDRMILIKGSRGIQLEKA
                     IPVFNLTT"
BASE COUNT         1168 a          850 c          648 g         1139 t
ORIGIN      
        1 gaaaagtgaa tcaacggcca agccaagatc ggcaccttcc tgatcatatg ttttgtcaga
       61 gttccaattc aaggatggga attggtttac taaatttaaa aattttgagt aatcgtattc
      121 atccgtggta gaagttaaaa tttcatttgc attgagcaaa ggttcaacaa cccggtcaat
      181 tctatgtata aaaccgtttt cagcaaaaat ctcctgtccc agaagttttg catcaacgta
      241 atacatatcg tcggcattat cgaattgccg gtcaaaatag aaagcataat cggaaagctg
      301 caaattataa attccgaaat attcagcata aaaaatcgga gcgaactttc gcgaatcagt
      361 aataacccta cgattccagg ttgtcgttga agagtcaata atcataagca tcttcttttc
      421 ttcatccaat actttgactc cgcatttgaa gttatcgcgc agcaacaagg tttgcctctt
      481 aaaccctcgt ggttcatcat tttcctcatc ttccgggtct atccaaccat taacgtcaag
      541 tgaacgtaac tgatcccggc tccatgggtt ttgtacaata tgatatttaa ctatctctaa
      601 tagctttgct ctgggaatat cgtcaaccga actataatta ttcttttgca agtactgttt
      661 tattgcatca ttggttggcg caaatacggt ataactcccc gaaacgttaa ttacagaatc
      721 gtagccaacg atctttatgc atttagcaaa ttctgaaagt tcatcaacat cctcaatttg
      781 agaatatact ttccctgcta accattcagg acgggcatat ttatcttggt cgtctttgca
      841 tgaaaaaaat atgatcagaa gcaccagaat gctccataaa aatttccttt tagccattgc
      901 gtcttgtcat tttaaaatga taacactttt taacaaactc caaaaatatt aaaaaaaaag
      961 tattcgagat ggttaaaagt tgcgaagata tgattttaac agtccgtaaa tcaatatcct
     1021 gtaaataaga agaatacaat taacaatgat acttttaacc ccgttcggtc agtgttttta
     1081 ctcggaatta actcaaatta ttgagtacaa ctttttgagg ttgcacaatt ttataccttt
     1141 accctttctg attctttgga agcaagccca attcttttcc ttgcttcagc atcagctgaa
     1201 atgcttcttc aaagttgttg tgtatctcac catccagaat tgcttccttt atggtgtttt
     1261 tgataattcc aacttcccgg cccggaccaa tcccaaatgt actcataata gtttcgcccg
     1321 ataccggcgg ctgaaaatta cgaatggcat ctcgctcctc aatatccttc agtttgctgc
     1381 gtaccagctg gaaattattc aggaaagtat ttactttttc ttcgttcttc gaagtgatat
     1441 ccgcctcaca aagagtcata agcaagtcga tatcatctcc tgcctcgaaa agtaaccgcc
     1501 ttacggccga atcggtaact tcttcttcac taagaacaat tggcctcatg tgcaagccca
     1561 ccatcttctg gacaaacttc attttttcgt tgagaggaag tttaagtcgc ttgaaaatgc
     1621 caggaataat tttagctccc aggtagttat ggccatagaa agtccacccc tgcccttcaa
     1681 caaacttctt ggtaagaggc ttggcaatgt cgtgtaaaag agctgaccat ctcagccata
     1741 agtcgtcgga tttttttgac aattggtcga gtacttccag tgtatgatag aaattgtctt
     1801 tatgacctat cccgtttttt acctcaacac ctttcatcct ggaaagttcg ggcaatatat
     1861 acgccaggag ccccgcttta tccatgagtt taaaacctac cgagggcttt accgacagca
     1921 ttattttgtt caactcgtca acgatccttt cttttgaaac gatagagatt cgttcgctat
     1981 attttgcaat tgcattgaac gtttcctctt cgatagaaaa gctcaactgg gctgaaaacc
     2041 gaatggcacg catcattctc aaagggtcgt cgtcgaaagt tatctccgga tcgagaggcg
     2101 ttttaatcaa ttttatatcg atatgatgta gtccattaaa cggatcgatg agttgcccaa
     2161 aagaatttcc gtttaggcta attgccatag cattaattgt gaaatcgcga cgatttaaat
     2221 cgtcttccag cgttcctctt tcaacaactg gctttcgcga atctctggag tacgattctt
     2281 ttcttgctcc aacaaattca aaatcaagcc cctggtaatt gaactgtgcc gttccaaaat
     2341 ttttgaaata attcactttt atgcgtggat taagagcatg ggcaactttg cttgccaact
     2401 caataccatc gccagtggta acaaaatcga tatctttaga tggcctgcca agaaccaagt
     2461 ctcgcacaaa accgccaact acataggcct caacccccaa ctcctgtgct tgttttgcaa
     2521 cagccttgaa aacaacagaa tcaatatgct ttgaaaaatc catcattgct tgctccctga
     2581 caataaaatg ctatgtgaca aaagtaacag aaaaatgaaa aacgccgctc tatctttgtg
     2641 gtgtaataca aataacacaa atactaaaca actaaaaaca aatactatgt tagaacattt
     2701 aactatcgac acattcaaaa ataaagtttt caactttgag gaaaataaag attggaaatt
     2761 tgaaggcgat gccccttgct taatcgactt ttatgccgat tggtgcggcc cctgtaaaat
     2821 gattgcccct gtgcttgaac aacttcagca agaatacggt aacaagctaa atatttacaa
     2881 agtaaacacc gaagacgaac gtgaattagc tgcgatgttc ggcatacaaa nnnnnnnnnn
     2941 acaaagcatt ccgtcgctat tgtttgtccc caaagaaggc aaaccacaaa tggcaatggg
     3001 tgctcttccg aaagaaactt tcgagcaagc gtttaaagaa gttttgggtg taacaaaaga
     3061 ctaatcaggt cccgccaacg cgggactttt ttttgccttt gaacaaacta gccggagtga
     3121 agttttaaaa tatctaagtt gtcaagttaa aaaccggaat tgctttttcc aattgtattc
     3181 ccctcgaacc cttaatgaga atcatccgat cttggtaatt catcttcccc aacacgggca
     3241 acaaatcatc aacccgatca aatttctttg cagaatcagg agctacctgc gaaaaattcc
     3301 taccaatcca atacacatca tcaatacccg acgacaaaac caactctaca atttttctgt
     3361 gttctgtctc cgattcatcg ccaagctcaa gcatatcgcc aaggatcata gtcttcgact
     3421 ggcttttcat ttgcataaaa ttccgtaccg aaacctccat actcgaaggg tttgcattat
     3481 aacaatctac aataatcaca ttgctgttta gtttcatcac ctgagaccgg ttatttgaag
     3541 gtacataatt ttctatttgc ttttgaataa gcaaagggtc aacattgaaa tatgaaccaa
     3601 cacaacaagc ggccaacaca ttttcgagat tatatgctcc tgtaaggttt gttttaatgt
     3661 agagccaacc cttggggaat aatactttcg caaccagcag caacccatca gtggcaactt
     3721 caccgactaa cattgcgttg ttaccagcct gtgaataagt gattttctca actccctccg
     3781 gaagcatctt taataaatgc tcattcccga catta
//
LOCUS       DAOPUW010000109         3721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_25117_length_3721_cov_4.279324, whole genome shotgun sequence.
ACCESSION   DAOPUW010000109 DAOPUW010000000
VERSION     DAOPUW010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25117_length_3721_cov_4.279324"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /gene="rpsB"
                     /locus_tag="PKJ43_02805"
     CDS             <1..389
                     /gene="rpsB"
                     /locus_tag="PKJ43_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNZ71519.1"
                     /translation="RLPAALFVVDVMKEKIAVREAQRLGIPVFAMVDTNSDPKGIDFV
                     IPANDDASESIKLIVGEMCDSIAEGLSERKSEREKETTDKKDQKSKSKKSPAKIDDDI
                     DDEEDEDIDEDDDDSDVADAIDEDEE"
     gene            424..1248
                     /gene="tsf"
                     /locus_tag="PKJ43_02810"
     CDS             424..1248
                     /gene="tsf"
                     /locus_tag="PKJ43_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNZ71520.1"
                     /translation="MADISAADVMKLRKLTGAGMMDCKSALADANGDFDRAIEIIREK
                     GKAIANKRADREASEGCVLAKVAGKKAGIIVLNCETDFVAKNQGFIDLTEKMLDVAIA
                     SNASNLEEALALTMDGRTLADHVIEQTGVIGEKIQLSYYSKIEAESVVAYIHPGNKLA
                     TVIGFNQANVDTQVSKDVAMQAAAMSPIAVDKDDVPAEVVEKELEIAKEKFRQEGKPE
                     AMLDKIAQGALTKFFNESTLLNQSFVKDAKVTIRQYLASNDKDLTVTSFLRYALSE"
     gene            1329..1742
                     /locus_tag="PKJ43_02815"
     CDS             1329..1742
                     /locus_tag="PKJ43_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNZ71521.1"
                     /translation="MENKQNTFRKEERLCNRIVIDKLFTDGQMVFCFPFRFVFLVTES
                     EEQPYPVKVVFSVPRKNFKKAVDRNLIRRRMREAFRTGKHPFYEMLRNNNKNVALMVI
                     YTNKEIAEYPFIQKSLIKGMKKMMDTILEKKNKLQ"
     gene            1764..2435
                     /locus_tag="PKJ43_02820"
     CDS             1764..2435
                     /locus_tag="PKJ43_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71522.1"
                     /translation="MRKAFTTLLALMAFNLLFSQNLLRENIFIEKNIPATLSELDSID
                     TEGTPYFNPEFTKGSFTVGNTSYIADMRYNIYIEMIEVQQGNTIVYLNPALKPKISMG
                     EKTFVYLNYPEKTMVYELLASQKNAMLVKKHQIEYIPIKWNKNGSIKKYPVYKKTNPA
                     IYLSPDQKELFNVEKTTDFISLFPREKENIKNFIKQEKLKKKKEADLIKLFNYVAGLT
                     EANQR"
     gene            2552..>3721
                     /locus_tag="PKJ43_02825"
     CDS             2552..>3721
                     /locus_tag="PKJ43_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ71523.1"
                     /translation="MKKISLYKYLVALFTFTVLFSCSVEKNTFVNRNYHNLTAHYNVY
                     FNGNEAMKAGLLKIETQVEEDYTKILPVYKESLPKTEALVSSDMTTAIEKGTKLIKLH
                     SITKPPKKKSKSGRYKKSEIKSEYNRWVDDAYLMMGRAYLYKKEYIMATSTFQLIIRK
                     YKNDPVRFDAYLWLIRSLSEDSRFSEATQLVQMLESDKEFPKKLEGELAIVSADMHMK
                     QQHYDEAIHSLDIGIKKIKGNKRKSRYTFILGQLYQETGKRDQALAAYKQVIRRSPEY
                     QMLFNARIRSAQVLSGEGNIAQLRKDLNKMRKKKWNQPYLDQIYYALGNISFNEGKID
                     DAIELYKKSAAVSRNNIHQKALSSLTLGDIYFDRKQYIPSGAYYDSAMTIIDESYP"
BASE COUNT         1316 a          778 c          735 g          892 t
ORIGIN      
        1 cccgccttcc agctgctctt ttcgtagtag acgtaatgaa agaaaaaatc gctgtacgcg
       61 aagcacaacg tcttggcatc ccagtatttg ccatggttga tacaaattca gatcccaaag
      121 gaatcgactt tgtaatacct gcaaacgacg atgcatcaga atcgattaaa ctgattgttg
      181 gcgaaatgtg cgattcgatt gctgaaggat taagcgaacg caaatcggaa cgcgaaaaag
      241 aaacaaccga caaaaaagac caaaaatcga aatcaaagaa aagcccggca aaaatcgacg
      301 atgacatcga cgatgaagaa gatgaagata tcgacgaaga tgacgatgat tcagatgttg
      361 ccgacgcaat cgacgaagac gaagaataat taattgaatt ttcatttcaa aaaagaaaaa
      421 gttatggctg atatatctgc tgcagatgta atgaaactgc gtaaattaac tggtgccggt
      481 atgatggact gtaaatcagc cttggccgat gccaatggcg atttcgatcg tgcaattgaa
      541 attatccgtg aaaaaggaaa agcaattgcc aacaaacgtg ccgaccgcga agcttccgaa
      601 ggatgcgtgc ttgctaaagt tgcaggtaaa aaagccggta tcatcgtatt aaactgcgaa
      661 accgactttg ttgccaaaaa tcaaggattt atcgatctaa ctgaaaaaat gcttgatgtg
      721 gctattgcca gcaatgcttc taatcttgaa gaagcccttg cattgactat ggatggccgt
      781 actctcgctg accacgtgat tgaacaaaca ggtgttattg gcgaaaaaat ccaattatcc
      841 tattattcaa aaatcgaggc cgaatcggtt gtggcttata tccacccggg aaacaaatta
      901 gctactgtta ttggttttaa ccaagcaaac gttgatacac aggtgtctaa agatgttgcc
      961 atgcaggctg ctgctatgag cccaattgct gtcgacaaag acgatgtacc cgctgaagtt
     1021 gttgaaaaag aactcgaaat tgctaaagaa aaattccgtc aggaaggaaa accagaagca
     1081 atgctcgaca agatcgccca gggtgctctt accaaattct tcaacgaaag cacattgctc
     1141 aaccagtcgt ttgtgaaaga cgctaaagtt accatcagac aataccttgc ctcgaacgac
     1201 aaggatctga ctgtaaccag tttcttacgt tacgcattaa gcgagtaata caaaactcaa
     1261 ttcaatattg aaaccgccgc aattcgttac ggcggttttt ttgtatttta gctgactgaa
     1321 aaatatagat ggaaaacaaa caaaatacgt ttagaaaaga agaacggttg tgtaaccgaa
     1381 ttgtaatcga taaacttttt accgatggac aaatggtttt ttgctttcca ttccgttttg
     1441 ttttcctggt taccgaaagc gaagaacaac cgtaccctgt aaaagtggtt ttttcagttc
     1501 cacggaaaaa tttcaagaaa gctgtagacc ggaacctcat tcgccgacgt atgcgggaag
     1561 ccttcaggac aggaaaacat cccttttatg aaatgctgcg caacaataat aaaaacgtcg
     1621 cactgatggt catttacacc aacaaagaaa ttgcagagta tccattcatc caaaagtccc
     1681 tgataaaagg aatgaagaag atgatggaca caatactcga gaaaaagaat aaattacaat
     1741 aaaatcaact gattacacga aacatgagaa aagcatttac aaccctgcta gccctaatgg
     1801 ctttcaacct gctattttcg caaaacctgt taagagaaaa cattttcatt gaaaaaaata
     1861 taccggcaac cttgtcggag ctcgacagca tcgacaccga aggcacacct tatttcaacc
     1921 ctgagtttac caaaggatca ttcaccgttg gaaataccag ctatatcgcc gacatgcggt
     1981 ataatatcta tatcgaaatg attgaagtac agcaaggcaa taccatcgtt tacctcaacc
     2041 ccgcgttaaa accaaaaata agcatgggcg agaaaacctt cgtgtacctg aattaccccg
     2101 aaaaaacaat ggtgtacgaa ctattggcca gccaaaagaa tgcaatgctg gtaaaaaaac
     2161 accagattga gtatatcccc ataaagtgga acaaaaatgg cagtattaaa aaatatcctg
     2221 tatacaagaa aacaaaccct gcaatttatc tctctcccga ccagaaagaa cttttcaacg
     2281 tggaaaaaac cactgatttt atttcattgt tcccccggga aaaagaaaac atcaaaaatt
     2341 tcattaagca agaaaagtta aaaaagaaaa aagaagccga tctgatcaag ctattcaatt
     2401 atgttgcagg gttaactgaa gccaatcaga gataatcttc tgaaagtttc gtataattgc
     2461 ataattttta gaaaagccct gggctaacat gtacaatata tttatgtttt tagagtttta
     2521 gttttcggta caaaaatttg acttataact tttgaaaaag atttcattat ataaatacct
     2581 tgttgctttg tttacgttta ccgtactgtt ttcatgttcg gtagaaaaaa acacatttgt
     2641 taaccgcaac taccacaacc tgactgcgca ctacaatgtt tatttcaacg gaaacgaagc
     2701 aatgaaagcg ggtctgttaa agattgaaac ccaggtagaa gaagattata caaaaatcct
     2761 ccccgtttat aaagagagcc ttccgaaaac agaagccctt gtttcgtcgg atatgaccac
     2821 agccattgaa aaaggcacaa aactcattaa actgcactca ataaccaaac cacctaagaa
     2881 aaaaagcaaa tcgggacggt ataaaaagtc cgaaattaaa tcggaataca accgctgggt
     2941 cgacgatgcc taccttatga tggggcgtgc ctatctgtat aaaaaagaat acattatggc
     3001 gaccagcacc ttccagctga tcatcagaaa atataaaaac gatcccgtgc gtttcgatgc
     3061 ctatttatgg cttatcagga gccttagcga agacagtcgg ttttcagaag caacacaact
     3121 ggtgcaaatg ctcgaaagcg acaaagaatt tccaaagaaa ctcgaagggg aattggccat
     3181 tgtaagtgcc gacatgcaca tgaaacagca gcattacgat gaggccatcc acagccttga
     3241 cattgggata aaaaagataa aaggcaacaa acgtaaatcg agatatacct tcattttggg
     3301 gcagctatat caggaaaccg gcaaacgcga ccaggcactg gctgcttaca agcaggttat
     3361 acgccgcagc cccgaatatc agatgctttt caatgcgcgc atcagaagtg cccaggtact
     3421 ctcgggcgaa ggcaatattg cgcaactgcg caaagacctg aacaaaatgc gcaaaaagaa
     3481 atggaaccag ccatatctcg accaaatcta ttacgcactg ggaaacatca gtttcaacga
     3541 gggaaagatt gacgacgcta tagaacttta caagaagtcg gccgctgtgt cgcgtaacaa
     3601 tattcaccaa aaagcactta gcagcctgac acttggtgat atttatttcg accggaaaca
     3661 atacattccc tccggagcat actacgacag cgcaatgact ataattgacg agagttatcc
     3721 a
//
LOCUS       DAOPUW010000110         3718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_25152_length_3718_cov_2.908272, whole genome shotgun sequence.
ACCESSION   DAOPUW010000110 DAOPUW010000000
VERSION     DAOPUW010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25152_length_3718_cov_2.908272"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..487)
                     /locus_tag="PKJ43_02830"
     CDS             complement(74..487)
                     /locus_tag="PKJ43_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71524.1"
                     /translation="MSHRFIEYQPYKGEIEKRTNGSLIALETGTAIAYALDKLQDRGR
                     YFVNPGDEVYEGMVVGENSREDDITINITKGKKLTNMRASGSDDKVKLAPPIIFTLEE
                     ALEYIQEDEYVEVTPKNIRIRKILLKEVDRKRNKK"
     gene            complement(497..1867)
                     /locus_tag="PKJ43_02835"
     CDS             complement(497..1867)
                     /locus_tag="PKJ43_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNZ71525.1"
                     /translation="MQIRNIAIIAHVDHGKTTLVDKMLMAGKLFKDHEKPGELIMDNN
                     DLERERGITILAKNVSVVYKDIKINIIDTPGHADFGGEVERVLNMADGVLLLVDAFEG
                     PMPQTRFVLQKAIQLGLKPIVVINKVDKPNCRPEDVHEMVFDLMFSLNATEEQLNFHT
                     VYGSAKEGWMSEDLSKKTTNINALLDAIIEHIPSPNVDKGTTQMLITSIVYSPYLGRI
                     AVGRLQRGEIKENQMVSLCHRDGSIVRTRIKELNLFVGLGRVKVESVSAGDICALVGI
                     ESFDIGDTIADIENPAPLPPIKIDEPTMSMLFTINNSPFYGQDGKYVTSRHIYDRLQK
                     ELEKNLALRIDQSPNTDSWNVYGRGVLHLSVLIETMRREGFELQVGQPQVIFKNIDGV
                     KSEPVEELTGDVLDEYSGRVIEMVSRRKGEMLSMERNNDRTVLEFHIPSRGIIGLRNN
                     ILTATA"
     gene            complement(2014..2610)
                     /locus_tag="PKJ43_02840"
     CDS             complement(2014..2610)
                     /locus_tag="PKJ43_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNZ71526.1"
                     /translation="MKHIIIIVVLLSVFRGAFSQSPINIGIKAGINSSSLIYNIDDFF
                     DQSQVNNYLVGAFARITFSKVYLQPEAYFNAKGGILSSPDPLYSLITEEITYNTIDVP
                     ILLGYKLIDKPMFNFRVNAGPVLSWVTTEPMIKELSDFNVNELKDNYLGIQAGIGMDI
                     WMISLDARVETSSNIFIDKSNYSASNRVYLITAGIKLF"
     gene            complement(2713..3219)
                     /locus_tag="PKJ43_02845"
     CDS             complement(2713..3219)
                     /locus_tag="PKJ43_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNZ71527.1"
                     /translation="MSTVHFNQQLLSLEHNLKYYALSLTSDRDRASDLLQETMLKALT
                     YKDKFSADTNFKAWVYTIMKNTFINDYRKASKSRNSLNGSNSEFHLKIAQDKVYPSPE
                     SFYSSNEIEKSISSLEDEYRIPFQMFLDGYKYKEIADKLELPLGTVKSRIFFTRKKLS
                     STLKDFVK"
BASE COUNT         1052 a          713 c          705 g         1248 t
ORIGIN      
        1 gcaaccatcg agtgggaata aaaaaaggct gtagtggtat tcactgcagc cttttttggt
       61 atgtcgaaat tttctatttc ttatttcgtt tcctgtcaac ctcttttagg agaatctttc
      121 tgattcttat gttcttaggg gttacttcaa cgtactcatc ttcttgtata tactcgagtg
      181 cttcttcgag agtaaagatg ataggagggg ccaacttcac tttatcgtct gatcctgaag
      241 ccctcatatt agttagcttt ttccctttcg taatatttat agtaatgtca tcttcccggc
      301 tattttcgcc tacaaccatt ccttcataca cttcatcgcc agggttaaca aaataccggc
      361 ctctgtcttg taatttgtca agggcataag caatggcagt tcctgtttct aaggcaatga
      421 gcgagccatt ggtccttttt tctatttcgc ctttataagg ttgatattca atgaaacgat
      481 gcgacataat tgcctctcat gcagtggcag taagaatgtt gtttcgaagt ccaatgatgc
      541 cccttgaagg gatgtggaat tccagaactg tgcggtcgtt gttgcgttcc atgctcagca
      601 tctcgccctt tcggcgtgac accatctcga taacccggcc cgagtattca tcaagcacgt
      661 caccagtgag ttcttcaacc ggttcgcttt ttactccgtc aatatttttg aaaataacct
      721 gaggctgacc aacctgaagc tcgaatcctt cgcggcgcat ggtttctatt aacacagaaa
      781 ggtgcagcac accacgccca tatacattcc atgaatcggt gttgggactt tggtctattc
      841 gtaacgcaag gtttttttca agttcttttt gaagcctgtc gtaaatatgg cgagacgtaa
      901 catattttcc atcttgcccg taaaaaggtg agttgttgat tgtgaacaac atactcattg
      961 tgggctcatc aattttgatc ggtggaagtg gcgctggatt ttcaatatca gcaatggtat
     1021 cgccaatatc gaaactttcg attcccacca aagcacaaat atccccagcc gaaaccgatt
     1081 caactttcac cctgcctaat ccgacaaaaa ggttaagttc ttttatccgg gtacgtacaa
     1141 tagaaccgtc tcggtggcaa aggctaacca tttggttttc tttaatttct ccacgttgaa
     1201 gacgaccaac cgcaattcgt ccaagataag gtgaatacac aattgaggta atcaacattt
     1261 gagttgttcc tttatctacg tttggcgaag gaatgtgctc aataatagca tcgagcagag
     1321 cattaatgtt ggttgttttt ttgctgaggt cttctgacat ccaaccttct ttggccgagc
     1381 cgtaaacggt atggaaatta agctgttctt cggtcgcatt taagctgaac attaaatcga
     1441 aaaccatttc gtgcacatct tcaggccggc aattgggttt atcaacctta ttgataacca
     1501 cgatgggttt taacccaagc tgaatggctt tttgtagcac aaagcgggtt tgaggcattg
     1561 gcccctcaaa ggcgtcgacc aaaagcaata ccccgtcggc catgttaagt acgcgttcaa
     1621 cttctccacc aaagtcggca tggccagggg tgtctatgat gttgatcttt atgtctttat
     1681 aaacaacaga aacattcttg gcaagaatgg taatccccct ttcccgctca aggtcgttgt
     1741 tgtccatgat aagctcacct ggtttttcgt gatctttaaa aagctttcca gccattagca
     1801 tcttgtcaac caatgttgtt ttaccgtggt ctacgtgggc aataatggca atgtttctaa
     1861 tctgcattct ctttttttta ggcagcgcaa aagtattttt ttttggttca atttgacagc
     1921 tattctaact aaatggggta gatgaaccca ttaaaaaacg tttttttaac aaagtggagt
     1981 atttccttaa ttaattattt gataagaaga taattaaaag agtttaatgc ctgcagtaat
     2041 taagtaaaca cggtttgatg ctgaataatt acttttatcg atgaagatgt ttgaactggt
     2101 ttctacccga gcatcgaggc ttatcatcca aatatccata ccgattcctg cttgtatacc
     2161 caagtagttg tcttttagtt cattgacatt aaagtcagat agttccttaa tcattggctc
     2221 tgtagtgacc caagacagga cagggcctgc attgaccctg aaattgaaca ttggtttgtc
     2281 gataagtttg tatcccaata agatgggcac gtcgatagtg ttgtaggtta tttcttcagt
     2341 tatcaacgag tagagtgggt caggagatga taaaattcca ccttttgcat tgaagtaagc
     2401 ctcaggttgc aggtatactt tgctaaatgt aatacgggca aaagcgccaa ctaggtaatt
     2461 gtttacctgg ctctggtcga aaaaatcatc gatgttatat atgagtgaag agctgttgat
     2521 tccagctttg attccgatgt ttattgggct ttgagaaaat gctccacgga aaacggaaag
     2581 taatactact attataataa tgtgtttcat ggtttccagc ttaaatgtaa aattacaaaa
     2641 ttatgccccg ttatacagca aaaaaaaggc cattgttttt caatgacctt tataacgtat
     2701 cgtaattctt gtttatttca caaaatcttt caatgtcgac gagagttttt ttctagtgaa
     2761 gaatatcctg ctcttaactg ttcccaaggg caattcaagc ttatcggcta tttctttgta
     2821 tttataacca tctaaaaaca tctggaacgg aattctgtat tcgtcttcaa gtgacgaaat
     2881 acttttttct atttcgttcg atgaataaaa cgattccgga gatggatata ctttgtcttg
     2941 ggctattttc aggtgaaatt cactgttcga tccattcaaa ctatttctcg acttcgatgc
     3001 ctttcgataa tcattaatga aagtattttt cataatggta taaacccatg ctttaaaatt
     3061 ggtgtcggcc gagaacttgt ctttatatgt caatgccttt agcattgttt cttgaagcag
     3121 atcacttgca cggtctctat ctgaagtaag acttaaggca taatatttca gattatgctc
     3181 aaggcttaac aattgctggt taaaatgaac tgtagacata atttataggt tttcggtgtt
     3241 aaacatggta caaagataag aaataaataa gacaaaaaca tctttaattc ttaatttttt
     3301 attaattagt gttaattgtt attagtcatt attcatttcg aagaataatt taagactttt
     3361 tttaatgcaa cgcaactttt tgctgtctta tcatttgttg ataatcaatt atttattgtt
     3421 ctgttgaaat cagggtgtgg tttacaatac tgtttgaaat cttattaaca tttgggtaat
     3481 gttcttccaa aaattgtcat tatatagtaa ggaaagaatt ataaaaaaga tcaaccattc
     3541 aaaatacctt atttttgtac cgataacttt aaacatttac tatgaattat tcactcgcaa
     3601 tgaaaaaacc gacggttgtt ttccgacgat ggaacaacaa acggtatgca gttttcaaca
     3661 gcttaaaaag gtcaatcaag attggggcac tattgattgc ctatttaaaa tttgtcaa
//
LOCUS       DAOPUW010000111         3716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_25175_length_3716_cov_3.898662, whole genome shotgun sequence.
ACCESSION   DAOPUW010000111 DAOPUW010000000
VERSION     DAOPUW010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25175_length_3716_cov_3.898662"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PKJ43_02850"
     CDS             <1..360
                     /locus_tag="PKJ43_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71528.1"
                     /translation="WTIEIDTGSGKIKAYESEEKKDGSYGVLKVFNESGSSAIYTYEW
                     TFDSAGNGSIIWKDATESFVYDVTVNVNKSGEAKWISNGDLFYHFSWNSDGSGSYYYY
                     GDNGEVLLSESWTVNDL"
     gene            577..1908
                     /gene="miaB"
                     /locus_tag="PKJ43_02855"
     CDS             577..1908
                     /gene="miaB"
                     /locus_tag="PKJ43_02855"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNZ71529.1"
                     /translation="MKKRLYIETYGCQMNVADSEVVAAVMAEKGYEITEDQHEADAIF
                     INTCSVRDNAEQRIWGRLRLFNSYKRKKKDLIIGMIGCMAERLADELLDKKAVDLVAG
                     PDAYRDLPFLIDKANEGETAINVELTIDETYDSLVPKRLDTDSITGFVSITRGCNNFC
                     TYCIVPYTRGRERSRDPKDILSEVADLVANGYAEVTLLGQNVNSYKWQPEGESKVMRF
                     PDLLKTIALSQPSLRVRFTTSHPKDLSDKLLKVMASHPNICKHIHLPFQAGSSRILKL
                     MNRKYDREWYLKRIAAIREILPGCGLSTDVFCGFCTETEEDFLETLSLMEEVRFDSAF
                     MFKYSERPGTYSAKYMKDDVSDEVKSERLSRMIELQNRISLENNRQDIGKTFEVLVEG
                     RSKKSEKDLFGRSSQNKVVIFPGENYKRGDLVNVTIERCTQTSLIGKVVSA"
     gene            complement(1920..3098)
                     /locus_tag="PKJ43_02860"
     CDS             complement(1920..3098)
                     /locus_tag="PKJ43_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71530.1"
                     /translation="MLKKILPLVIMLTAALLCSAQELDELIKEIHSEPKTQNKCLQMA
                     NMVMRYLNEDKADSVHAIISRWENSEGTSEPIFRAKMLLALKEGKNTDSLTDKNTIDH
                     IFKYISRIDLIQYNNLAEYEEFRSDCGNIEPGGTFDQFTWETASTLKHKFAPNSLPYL
                     WCEFYGENCDTLLSKIQGKEFANTMLAKQHSKQVNKYLNYSEFHIALLSGLWIPTGEL
                     KVLGIHPEIGLLSGWKKNKMNYDLAFSMKFCDTPKPYWATRHGKTSPEQTQMFIGGYF
                     AFETSYDLISHKKHSLQLATGIGIDGFDAFRSDSLMNLEAASTNTFNFNAGFSYRYYY
                     KKNTYIGARVKYNIVDYTRSGVIDLTGNTLSFQVVIGGLENSVKYQQLDLLRYPLRGH
                     "
     gene            3273..3623
                     /locus_tag="PKJ43_02865"
     CDS             3273..3623
                     /locus_tag="PKJ43_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNZ71531.1"
                     /translation="MGKLVDEFNVYRTKMNEKILASDNKVMKRIYGVDTLAYQDGALS
                     VKVKEMLGLATSMVLRCDDCIKYHLGKCYEQGVSTEEIFEVFSIANLVGGTIVIPHTR
                     RAVEYWEELLAKAD"
BASE COUNT         1076 a          632 c          808 g         1200 t
ORIGIN      
        1 tggacaattg aaatcgacac cggttcaggg aaaatcaaag cttacgagtc agaagaaaaa
       61 aaagatggct catatggtgt gctaaaggtt ttcaacgaat cgggtagtag tgccatttat
      121 acttatgagt ggacgtttga ttctgcagga aatggatcaa ttatttggaa agatgctact
      181 gaatcatttg tttatgatgt tacggtaaat gtaaacaaat caggagaagc aaagtggata
      241 tccaatggcg atttgttcta tcatttctct tggaactccg acggaagcgg gagctattat
      301 tattatggcg acaatggcga agtcttactt tctgagtctt ggacggtgaa tgatttgtaa
      361 attaatttct tatgaatagt gaaaaacttg ttggctttaa agcaacaggt ttttcttttg
      421 tctatttgat accgtttttc taaagaattt ttaaatttag tacatgcagg tcttattttt
      481 ttgttatcct tctttttcac tacttacaat taaaggccaa agattttcgt ttcggaaaat
      541 gaaattaact ttgcaatcct gtttaaggtt aaagcaatga agaaaagatt gtatatcgag
      601 acctatgggt gtcagatgaa tgttgccgat agtgaagtgg tggcagctgt gatggccgag
      661 aaagggtatg aaataactga agatcagcat gaggctgatg ctatttttat taatacttgt
      721 tcggttcgcg ataatgctga acagcgcatt tggggacggt taaggctttt caattcgtat
      781 aaaagaaaaa agaaagatct cattattgga atgattggtt gcatggctga gcgcctagcc
      841 gatgaactac ttgataaaaa agccgttgat ttggttgcag gtcccgatgc atatcgtgat
      901 ttacctttcc tgatagataa agccaatgag ggcgaaactg caataaatgt agagctgact
      961 attgacgaaa cttacgattc attagtgcca aaaaggcttg acacagattc tattacgggt
     1021 tttgtttcaa ttacccgagg gtgcaataat ttctgtacct attgcattgt tccttacacc
     1081 agaggccgag agcgtagccg ggatcctaag gatattctta gcgaagtggc cgacttagta
     1141 gccaatggct atgcagaagt gaccttgttg ggccaaaacg tgaattcgta caaatggcaa
     1201 cccgaaggtg aatcgaaagt gatgcgtttt ccagacctgc tcaagacaat tgccttgagc
     1261 caaccctcat tgcgagtgcg ttttactacg tctcatccca aagatttgtc cgataagttg
     1321 ctaaaggtga tggcatcgca ccccaatatc tgcaaacata ttcatttacc ttttcaggct
     1381 ggaagttcgc ggattctcaa attaatgaac aggaaatatg atcgtgaatg gtatttaaaa
     1441 cgtattgcgg caattcgtga aattcttccg ggttgtggcc tttcaaccga tgttttttgc
     1501 ggtttttgta ccgaaaccga ggaggatttt cttgaaacac tctcgctgat ggaagaagtt
     1561 cggttcgatt cagctttcat gtttaagtat agcgaacgtc ctggaacata ttctgcaaag
     1621 tatatgaaag atgatgtctc agacgaggtt aaatcagaac ggttatctcg gatgatagaa
     1681 ctccagaacc gaatttcgct tgagaataac agacaggata tcggcaaaac ttttgaggtt
     1741 cttgttgagg ggcgttctaa aaaatcggaa aaagatcttt ttggccgttc gtcgcaaaat
     1801 aaagtggtga ttttccccgg agaaaattac aaacgtggcg atttggtgaa tgtaaccatc
     1861 gaacgctgta ctcaaacttc gctaataggg aaagtagtat ctgcttgata ttctaatatc
     1921 taatgccccc ttagtgggta gcgcaataag tcgagttgtt ggtattttac actgttttct
     1981 aagcctccaa taacaacttg aaaagacaag gtatttccag tgagatctat tactccgctt
     2041 cgggtataat caactatatt gtatttgact cttgctccaa tataggtgtt ctttttatag
     2101 taataccgat aactaaatcc agcattgaaa ttgaacgtgt tggttgatgc tgcttccagg
     2161 ttcatcagcg aatcggatct aaatgcgtca aaaccatcga tgccaatgcc tgttgctaac
     2221 tgaagactat gctttttgtg agaaataagg tcgtagctgg tttcaaaagc aaaatacccg
     2281 ccaataaaca tttgggtttg ttcggggctt gttttcccat gtcttgttgc ccaataaggt
     2341 ttgggtgtat cgcaaaactt catcgaaaat gctaaatcat aattcatttt gttcttcttc
     2401 catccgctca ataacccgat ttcggggtga atgccaagca ctttcaactc gcctgtggga
     2461 atccataatc cagaaagtag ggcaatatga aattcgctat agttcaggta tttgttcact
     2521 tgtttcgagt gttgtttagc aagcatggtg tttgcaaatt cttttccctg aatttttgaa
     2581 aggagggtgt cgcaattttc tccgtaaaac tcgcaccata aatagggcag ggagtttggc
     2641 gcaaacttgt gtttcaaagt cgaagcggtt tcccatgtaa actggtcgaa agtgccaccg
     2701 ggttcaatat tcccacagtc ggatcgaaac tcttcatact cggctaagtt attgtattgt
     2761 attaagtcaa ttcggcttat atatttgaat atatggtcga tagtgttttt atctgtgaga
     2821 gaatcggtgt ttttaccttc ttttaaagcc agcagcattt tagccctgaa gattggttcc
     2881 gaagttccct ctgagttttc ccatcgactg attattgcat ggactgagtc ggctttatct
     2941 tcgttgaggt aacgcataac catattggcc atttgcaaac atttattttg cgtttttggc
     3001 tcagagtgaa tttctttaat taattcgtcg agttcttggg cagagcataa taaagcagct
     3061 gtaagcataa tgactaatgg taggattttt tttagcatag tttcaaattt tcagattata
     3121 caattatata aaatgtttcg ctaatgtcca cattttcaac aaaggcaaaa atgcatttta
     3181 attgtagaag aaaaagaaag caaatttttc gcttatcaaa aaaacccttt ttgtattttg
     3241 ttgttcaatt gtgaaattaa aaattaaggc acatgggaaa acttgttgat gaatttaatg
     3301 tctatcgtac aaaaatgaac gagaagattc ttgcatccga caataaagta atgaaacgca
     3361 tttatggtgt tgatacactt gcctatcagg atggtgcatt gagtgttaag gtaaaagaaa
     3421 tgttaggttt ggctacgtca atggtgttac gctgtgatga ttgcattaaa tatcatctcg
     3481 gtaaatgtta cgaacagggc gtatcgaccg aagaaatctt cgaagttttt tctattgcaa
     3541 accttgttgg tggtactatt gtaatccctc atactcgcag ggcagttgaa tattgggaag
     3601 agcttcttgc taaggcggat taattattaa gtagttcttc gatttttatt gttgtaaatg
     3661 gtcctttcga tgcaggcacc ttattaatgc ttaagtgaat tgttttgtat tcgggt
//
LOCUS       DAOPUW010000112         3704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_25293_length_3704_cov_3.681008, whole genome shotgun sequence.
ACCESSION   DAOPUW010000112 DAOPUW010000000
VERSION     DAOPUW010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25293_length_3704_cov_3.681008"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="PKJ43_02870"
     CDS             complement(<1..824)
                     /locus_tag="PKJ43_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010423522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNZ71532.1"
                     /translation="MELDILRDIVIIFALSTVVNLLFTKLKLPTILGYLLTGIIAGPH
                     LLKLIHDQHNIELMAEIGIVLLLFSIGLEFSLKHLFKIRRVVFLGGFMQVIFTAVVFM
                     FISFIYEISWTSGIFIGFMAALSSSALVLKILQDRSELTSNYGRTVLGILIFQDLMLV
                     PLLLFSDFLGKGATLDVGHELIVLLIKAIGIIAFVYVGNKWLFPRILHIIALAKNQEL
                     FMMSIFLICLAIALLTSSLGMSLAFGAFLAGLMVSESEYSHNAFGSLIPFKDLFSS"
     gene            complement(851..1231)
                     /gene="crcB"
                     /locus_tag="PKJ43_02875"
     CDS             complement(851..1231)
                     /gene="crcB"
                     /locus_tag="PKJ43_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNZ71533.1"
                     /translation="MLKNILIIGAGGFIGSVLRYLTQVASGKILSLSFPVGTFIVNIL
                     GCFIIGVVYALSEKNNFLTTEMRLFLAVGFCGGFTTFSTFSNDNLILLKDSQLAFLFF
                     NIFGSVILGVAAVYLGIIAVKTFL"
     gene            complement(1436..2377)
                     /locus_tag="PKJ43_02880"
     CDS             complement(1436..2377)
                     /locus_tag="PKJ43_02880"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HNZ71534.1"
                     /translation="MRKLLIVIFILATGYSAQAQLLRNIARSAANQAVNSVENRAEKE
                     VDKQVDKGVNKAIDKALEEDSTKQAAPSQKKQTEAEDPSSKIFKKLGISTEVAAHKED
                     YKFTGQIVMIMEITDEKGKKAGGGEYTTCFNETNSDAMFKLSGEGSESTTTIFDQENS
                     CMLILSESNGSKTGFATKFDPNAAQSANLPQTQNQQQTTNDSADDCTPSKTGKTKTIS
                     GYSCTEYSCETDEVSVSVWITKDFSAKNNKIFKNNSMGMNYKTDGLDGMVMQYESRSK
                     KDKSSSVMTVKSIDMKKSSSFSTKGYQFTGLNFGGAK"
     gene            complement(2529..2969)
                     /locus_tag="PKJ43_02885"
     CDS             complement(2529..2969)
                     /locus_tag="PKJ43_02885"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNZ71535.1"
                     /translation="MKYTEATTGRSFVLRLEDGEILHEVIEKFAEEQKIDAAKLIVLG
                     GADKGSILVTGPRDGRAPIIEKQEITLDDVHEITGVGTLFRNEEGKVISHIHLSAGRG
                     EKAVTGCARSGVKIWLVAEVVITELYNCTARREKDHNGFELLTM"
     gene            3147..>3704
                     /locus_tag="PKJ43_02890"
     CDS             3147..>3704
                     /locus_tag="PKJ43_02890"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HNZ71536.1"
                     /translation="MRNLTLFLLLFFVVEVFAQPKDDKLMGVIRTQPFFKNAGISICV
                     SDVLSDSVLWSYQPELCMVPASVQKLITSATALEVFGADMRFETVVWFNGTIESRKLS
                     GDLVVTGGGDPTLGSGFFCKQGEQQDFLAQWVHRFRQAGIDTITGDIVVDPFIFSDND
                     VPQSWLYEDVGNHFGAAATGLAIFDN"
BASE COUNT         1007 a          739 c          763 g         1195 t
ORIGIN      
        1 aagctcgaaa acaagtcttt aaaaggaata aggctaccaa aagcattgtg gctgtattcc
       61 gattcagaaa ccattaaccc cgcgaggaat gccccgaatg ccagcgacat acccaacgaa
      121 gaagtgagca gcgcaatggc aaggcaaata agaaagatgc tcatcatgaa aagctcttgg
      181 tttttagcca gcgcgataat gtgtaatatt ctcgggaata accacttatt cccgacatac
      241 acaaacgcga taatgccaat cgctttaatt agaagtacga taagttcgtg acccacatcg
      301 agggtggctc ctttgccaag aaaatcgctg aaaagcagca gcggaaccag cataaggtct
      361 tggaatataa gtattcccaa taccgttcgg ccatagttag aagtgagttc tgagcggtct
      421 tgcaatattt tcaaaactaa agccgaacta ctcagcgcag ccataaaccc gataaaaatt
      481 cccgaagtcc agcttatttc gtaaatgaat gagatgaaca taaatactac tgcggtaaaa
      541 atcacttgca taaagccacc aaggaatact acacgcctga ttttaaacag gtgttttaga
      601 gaaaactcga ggccaatgga aaacaggagt aacacgatgc caatttcggc catcaattct
      661 atattatgct ggtcgtgaat gagtttaagt aggtgtgggc cggcaatgat gcccgtgagc
      721 aagtacccca atatggttgg tagtttcaac ttagtgaaga gtaggtttac tacagttgaa
      781 agggcaaaaa tgatgactat atcccgtaaa atatctagtt ccatcttgtt atgtttccta
      841 aatcaattgg ttataagaac gtttttactg caataatgcc taaataaacc gcggcaacgc
      901 ctaggattac gctgccaaat atattgaaaa acaaaaatgc caactggcta tcttttagta
      961 atataagatt atcgttcgag aatgttgaaa aagtggtgaa gcctccgcag aaaccaaccg
     1021 ctaaaaagag gcgcatttcg gtggtgagaa aattgttctt ttcagaaagg gcatatacaa
     1081 ccccaatgat aaaacatcct aatatgttga cgatgaatgt tcccacagga aaagacaagc
     1141 ttaatatttt gcctgaagcc acttgtgtga ggtagcgtaa cacgctgcca ataaacccac
     1201 ccgcacctat tatcaatata ttctttaaca tataaccttt tttcaatgag caaaataaca
     1261 aaaaaaccgg ctgaaaaccg gttttaatga aatataaatt gtgattttta taccaagtgc
     1321 aatttcaaaa cagaccaaaa tcactcaggt ataatgccga tgaatagaaa tgtcgggcca
     1381 atcgagagag cgatcccgat taatcgggag gactgttaca tttcttcaat cggtattatt
     1441 ttgctccccc aaaatttaat ccggtaaatt ggtatccttt ggtcgagaat gaactgcttt
     1501 tcttcatgtc gatacttttt acagtcatta cagacgatga tttgtctttt ttcgagcggc
     1561 tttcatattg catcaccatg ccgtcaaggc catcggtttt atagttcatg cccatcgaat
     1621 tattcttaaa tattttattg ttctttgctg aaaaatcttt ggtaatccac acgctaaccg
     1681 atacttcatc ggtctcacat gaatattcgg tgcatgaata tccgctaatt gtttttgttt
     1741 tacctgtttt cgaaggggta caatcgtcag cactgtcatt agttgtctgt tgctgatttt
     1801 gggtttgtgg caggttggcg ctttgtgctg cattggggtc aaatttggtg gcaaaacctg
     1861 ttttcgatcc gttcgattcg ctgaggatca acatgcatga gttttcctga tcgaaaattg
     1921 tagttgtgct ttccgagcct tcaccactta gtttgaacat agcatccgaa ttggtttcgt
     1981 taaaacaggt tgtgtattcc ccgccgcctg cttttttgcc tttttcatcg gttatttcca
     2041 tgatcataac aatctggccg gtaaacttat agtcttcttt gtgtgctgca acttcagttg
     2101 aaatgcccaa tttcttgaat atttttgacg aagggtcttc tgcctcggtt tgttttttct
     2161 ggcttggtgc ggcctgtttg gttgagtctt cttccaacgc tttatctatg gctttgttaa
     2221 ctcctttgtc gacttgtttg tcaacttctt tttcagcacg gttttctaca gaattaacag
     2281 cttggttggc agcgctacgg gcaatgtttc tcaagagttg tgcctgggca gagtagcctg
     2341 tggcaagaat aaatataacg ataagtaatt ttctcatagt gaaatgtatt atggtttttt
     2401 tacaaattta gacagtttta tttaaaaaat taccttcttg tccccttatt ttgaggtcga
     2461 ttattattcg tcttgatgtg ggttgaaagc ggcgtgcttt gtactgaatt tgtgtgtttt
     2521 tattttgttt acatggtcag aagttcaaaa ccgttatggt ctttttctct tctggctgta
     2581 cagttataca gttcggtaat cactacttcg gccacaagcc aaattttgac ccccgaacgg
     2641 gcgcagcctg taactgcttt ttcgcctctg ccagccgaaa ggtgtatgtg tgaaatcact
     2701 ttcccttctt cgtttctgaa gagggtgcca actccggtga tctcgtgcac atcgtcgagc
     2761 gtaatttctt gtttttcaat gattggagct cttccgtcac gaggacccgt taccaagatg
     2821 cttcctttgt cagctccgcc cagcacaatc agctttgctg catcaatttt ttgttcttca
     2881 gcaaatttct caattacttc gtgaagaatt tccccgtctt cgagtcgtaa aacaaaactc
     2941 cgcccggttg ttgcttctgt atatttcatt ttttgttttt tttgctggtt caaaggtaaa
     3001 atgatttttt tgtagttttt ttaagctctg gcttttatca gatcttttta atgccagcat
     3061 tcgggattaa tcctgagttt tatggcacaa aataattcaa aataagattg ttatatttgt
     3121 tcccgtttga tataatctct ttgaatatga gaaatctcac cttgttttta ttactctttt
     3181 ttgtcgttga agtgtttgct cagccaaaag atgacaaact catgggagta atcagaacgc
     3241 agcccttttt caaaaatgca ggcataagta tttgtgtttc tgatgtgtta agcgactctg
     3301 tgttatggtc gtaccagcca gaattatgca tggttcccgc ttcggtacaa aagctgatta
     3361 cttcggccac ggcacttgaa gtttttggcg ccgatatgcg tttcgaaacc gtggtatggt
     3421 tcaacggaac gattgaaagc aggaaattgt cgggcgattt agtggtaacc ggtggtggcg
     3481 atccaacgct tggttcgggg tttttctgta aacaaggaga acaacaagat tttcttgccc
     3541 agtgggttca tcgttttcgt caggcaggta ttgataccat taccggggat attgttgtcg
     3601 atccatttat tttttctgat aatgatgttc cacaatcgtg gttatacgaa gatgtaggaa
     3661 accattttgg agctgcggct acaggacttg ccattttcga caat
//
LOCUS       DAOPUW010000113         3704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_25295_length_3704_cov_3.101672, whole genome shotgun sequence.
ACCESSION   DAOPUW010000113 DAOPUW010000000
VERSION     DAOPUW010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25295_length_3704_cov_3.101672"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1602)
                     /gene="ppk1"
                     /locus_tag="PKJ43_02895"
     CDS             complement(<1..1602)
                     /gene="ppk1"
                     /locus_tag="PKJ43_02895"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNZ71537.1"
                     /translation="MATYKYFNRDISWLSFNHRVLEEAGDRSIPLYERIKFLAIYSNN
                     LEEFYQVRVSYYRQLLQNADLIPAKIEEVQPARVLHKINEIVNGYLNYFNDIYNNQII
                     PELEENGIMLLERNATLNEQQSKEVKEIFMTEILSALQPVVLIKERIRPFLKTGQGYI
                     VTEMVINEPKRPKELKPRVRYGLIKVPTDHNISRFIELEPTEGKHYIMFLEDVIMRHI
                     NKIFPGYTILDWYTIKITRDADFEYDEYEEEELINAIGKISSSRKLGEANRFLYDSRM
                     PSKILNYLIHTFRIENDIVVQGSSYHNIQDFFRFPNPLSPKLENEKLIPLRIPELDKF
                     KWISDAIDKKDYLLSVPYQSYDYFLKFLEQAARDESVTEIKATQYRVAERSAVVNALM
                     NAAENGKKVTVFVELKARFDEEANLQYAAEMTKAGIHIIYSIPGLKVHAKLALVIRNK
                     ERFPDLKDQAYLSTGNFNEKTARLYCDHGLFTSHEGIISDLKKMFLYLEEQELQIHFE
                     HILVPNFNLVYKFGILIGQEIENVKQ"
     gene            complement(1687..2094)
                     /gene="arfB"
                     /locus_tag="PKJ43_02900"
     CDS             complement(1687..2094)
                     /gene="arfB"
                     /locus_tag="PKJ43_02900"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNZ71538.1"
                     /translation="MDIQNRNFLAEFTFLSSRSSGPGGQNVNKVNSKVELRFNIDKSD
                     QLSENEKEIILKKLKNRINSEGELLIAVQTDRSQLKNKELAIELFYNLITKALTPRKP
                     RKSTTPTRASVAKRLETKKQDSEKKMRRKKPDL"
     gene            2578..>3704
                     /locus_tag="PKJ43_02905"
     CDS             2578..>3704
                     /locus_tag="PKJ43_02905"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNZ71539.1"
                     /translation="MLFTEMGLSEDILMGIRELGFETPTPIQEKVIPILIENRRDIVG
                     LAQTGTGKTAAFGLPLLQNISLRDNYPQMLVLSPTRELCLQITRDLENFSKFKNGIKI
                     VAVYGGASMDKQISALKQGVHIIVATPGRINDLLRRKKADLSRINSLVLDEADEMLKM
                     GFREEVDAILASTPNEKNTLLFSATMSADIANIAKSYMNDPVEVSIGTKNAGADNVSH
                     IYHMVHAKDRYAALKRVADYNPDVYGIVFCRTRQETKEVAASLMKDGYNAEALHGDLT
                     QAQRDYVMQKFRDRNLSLLVATDVAARGLDVNDLTHVINYNLPDDSENYTHRSGRTGR
                     AGKEGTSVSIIHTKEMGKVREIERAIKKKFVPMPVPGGQEI"
BASE COUNT         1010 a          682 c          781 g         1231 t
ORIGIN      
        1 ttgtttaaca ttctcaattt cttgcccgat caatatccca aacttgtata caagattaaa
       61 gttgggaacc agaatgtgtt caaaatgaat ttgcagttct tgttcttcaa gatagaggaa
      121 catttttttt aagtctgaaa taattccttc gtgagaagta aacaacccat ggtcgcagta
      181 taaacgggct gttttttcgt tgaagtttcc agtgctcaga taggcttgat cttttaaatc
      241 gggaaatctc tccttatttc ggataacaag agcgagtttg gcatgtacct tcagcccggg
      301 aatactgtag ataatatgta ttcctgcttt ggtcatttca gcagcatact gaaggttggc
      361 ttcttcgtcg aatcgggctt ttaattcgac aaacacagtt acttttttcc cattttcagc
      421 cgcattcatc agtgcattta ccactgccga ccgttcggcc acgcgatatt gtgtggcttt
      481 tatttcggta acagattcgt cacgggcggc ttgctcgagg aactttaaaa agtaatcgta
      541 cgattggtat ggaacactta ataaataatc ttttttatcg atagcatcgc taatccattt
      601 gaatttatcg agttcgggga ttcgcaatgg aatcagtttt tcattctcga gttttggtga
      661 gagggggttt ggaaaacgga aaaagtcctg aatattgtgg tagctactgc cctgtactac
      721 aatatcgttt tcaattctaa atgtgtgtat caggtagttg agtatttttg aaggcatccg
      781 gctatcgtat agaaatcggt tagcttctcc caattttctc gatgaagata tttttccgat
      841 agcattgatg agttcttctt cttcatattc atcatattcg aagtctgcat ctcgggtaat
      901 ctttatggtg taccaatcga gaatagtgta tccgggaaat attttgttaa tatggcgcat
      961 aattacatct tcgagaaaca taatgtaatg tttgccttcc gttggttcca gttcgataaa
     1021 cctcgatatg ttgtgatctg tagggacttt tatcagcccg tatcgtaccc gtggttttaa
     1081 ttcttttggg cgtttgggtt cattgatcac catttcggta acaatatacc cttgaccggt
     1141 tttaagaaat ggccgtatcc gttcttttat aagaacaaca ggctggaggg cacttaaaat
     1201 ttcggtcatg aaaatctctt ttacctcttt tgactgctgc tcgttaagag ttgcatttcg
     1261 ttcgagaagc ataatgccat tttcttccag ctcgggaatg atctggttgt tatatatgtc
     1321 gttgaaataa tttaggtatc cgtttacaat ttcattgatc ttatgaagaa ccctggctgg
     1381 ttgaacttcc tcaattttag ctggaataag atctgcattt tgcagtagct gccggtaata
     1441 gcttactcta acctgataaa attcttcgag gttgtttgag taaattgcca aaaattttat
     1501 ccgctcgtat aacgggatcg atcggtctcc ggcttcctca agcacgcggt ggttgaacga
     1561 aagccagcta atatcgcggt taaaatactt gtatgtagcc aatttaagga atctttaagt
     1621 tcttaattct tcaaagttat tcgttttagg cgtaatcccg gtttttgtaa agaaatattt
     1681 tgttgcttat aagtctggtt tttttctacg cattttcttt tcgctatctt gcttttttgt
     1741 ttcaagcctc tttgctactg atgcccttgt tggtgttgtc gattttctgg gctttcttgg
     1801 tgttaaagcc ttggttatta aattgtaaaa tagttcaatg gcaagctctt tgtttttaag
     1861 ctgagacctg tctgtttgta cggcaattaa aagttcacct tcgctattga ttctgttctt
     1921 gagtttcttt aagatgattt ctttttcatt ttcagaaagt tgatccgatt tatcgatgtt
     1981 gaatcgaagt tcaaccttcg agttgacttt gtttacattt tgcccgccag gtccactgct
     2041 gcgtgacgat aagaaggtaa attcggctaa gaaatttctg ttttgtatgt ccattatttc
     2101 ggaatgtttt ttcaaagata gggaatagaa tgatagtctg ccaatagaaa tgcatcaaca
     2161 aaatgcatag tttttagtta gcatgcaatc ataaatagag tgagccgatg agatgagttt
     2221 aaagtttcat aatgggatat tgggtaattg cttgtctttc aatttttagt atgaatgagt
     2281 ttattttgcc aacacaacta actttacgtt aacaacacaa aaataaattg cataaaacaa
     2341 tattataagg ttaattgtgc tacttttgcg ccgcctttaa tgaaatctaa agttttaacg
     2401 ttcattacaa agcatttgtt ccaggtaaaa ctttttataa ggctttaagt tactatttag
     2461 atttttgtgt ctctggcaaa cgctggagga tcgggtggga acagtgctga atattaaccg
     2521 ggttaccctg tattctgtag gtaaaacagg aacgggaagg ccctaaaaca attttttatg
     2581 ttatttacag aaatggggct ttccgaagat attcttatgg gtatcaggga attaggcttt
     2641 gagacgccca ctccgattca ggagaaagtt attccaattt taattgaaaa tcgtcgtgat
     2701 attgttggac tggcacaaac cggtaccggc aaaactgcag catttgggtt gcccttgctg
     2761 caaaatatct cgttgcgcga caattatccg cagatgttgg tgctaagccc aacgcgtgaa
     2821 ttgtgccttc agatcacccg cgaccttgaa aatttcagca aatttaaaaa tgggatcaaa
     2881 atcgttgccg tatacggtgg agcatcgatg gacaaacaaa tcagtgcgtt gaaacaaggt
     2941 gtgcatatta ttgttgccac tcctggccgt ataaacgatt tattgagacg aaaaaaagct
     3001 gacttgtcca gaattaactc gttggtactc gacgaagcag atgagatgct gaaaatgggc
     3061 ttcagagaag aagttgatgc tattttagct tctacaccaa acgaaaagaa cacgttgctt
     3121 ttctctgcaa ccatgtcggc tgacattgcc aatattgcca agtcgtatat gaacgaccct
     3181 gttgaagttt cgattggaac aaaaaatgct ggggccgaca acgtgagcca tatctatcac
     3241 atggtacatg ccaaagaccg ttatgccgca ttgaaacgtg tagccgatta taatcccgat
     3301 gtatatggta ttgtattttg ccgtactcgt caggaaacca aagaggtagc tgcttcattg
     3361 atgaaggatg gatataacgc cgaagctctt catggtgatc tcacacaggc tcagcgtgat
     3421 tatgtaatgc agaaatttcg cgacaggaac ctgagtttgc ttgttgcaac cgatgtagca
     3481 gcccgtggtc tcgatgtgaa cgaccttact cacgtaatca attataacct tcccgacgat
     3541 tctgaaaact atacccatcg ctctggtcgt accggtaggg caggaaaaga aggaacttca
     3601 gtatccatta tccacactaa ggagatgggt aaggttcgtg aaattgaacg ggcaattaaa
     3661 aagaaattcg ttccgatgcc tgttcctggt ggtcaggaaa tttg
//
LOCUS       DAOPUW010000114         3673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_25571_length_3673_cov_3.559149, whole genome shotgun sequence.
ACCESSION   DAOPUW010000114 DAOPUW010000000
VERSION     DAOPUW010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25571_length_3673_cov_3.559149"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /gene="miaA"
                     /locus_tag="PKJ43_02910"
     CDS             complement(<1..792)
                     /gene="miaA"
                     /locus_tag="PKJ43_02910"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNZ71540.1"
                     /translation="MATTPYNLITITGPTASGKTSVAAHLARQINGEIISADSRQVYR
                     GMDIGTGKDLSDYMVDGLIVPYHLIDIADAGYEYNVFEYQRDFINAFNDIRQRGKFPI
                     VCGGSGMYIEAVLKGYKLINVPINEELRKSLAGKTLDELAEILKKYKTVHNNSDIETP
                     KRATRAIEIEEYYRQTPQQDRYYPEIKALIVGMLFDRETRRQRITERLKARFDEGMID
                     EIENLINNGVSPEALIYYGLEYKYIPRYVSGEISYNEMFSQLETAI"
     gene            861..1445
                     /locus_tag="PKJ43_02915"
     CDS             861..1445
                     /locus_tag="PKJ43_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71541.1"
                     /translation="MSLFQPFTTIYHESLAFFLHPQVYWRELKSGSRKNVFGFRNFLV
                     PGLIIGFVCIVLGDLIFHSKNGFFWQDSLVKACRKTLFIFMLLTFSVMITKFVLGQFH
                     FELKMKTARKIAVISLAPAVLKTVITGLLPFLDLGGVLPWYGFFLAYFGFETFFVIPD
                     EKKFYFYFALFMAIFSLIMFLTFILNRISAHILL"
     gene            1447..1908
                     /gene="smpB"
                     /locus_tag="PKJ43_02920"
     CDS             1447..1908
                     /gene="smpB"
                     /locus_tag="PKJ43_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNZ71542.1"
                     /translation="MALKPRTDVSIKNKKAFFEYEIIESFIAGIQLQGTEIKSIREGK
                     AGLVDSYCQFAGGELYVKNMHIAEYFFGNLRNHEAKRERKLLLQKKELRKLERKVRET
                     GLTIVPLKLFLNEKGFAKLEIGLARGKKVYDKRESLKQNDARRDMDREMKY"
     gene            complement(1920..2576)
                     /gene="nth"
                     /locus_tag="PKJ43_02925"
     CDS             complement(1920..2576)
                     /gene="nth"
                     /locus_tag="PKJ43_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNZ71543.1"
                     /translation="MNRKERFEKVLAWFELNMPVAETELQYSSPFGLLVAVILSAQCT
                     DKRVNQITPPLLEKYPTAQFMALATPEEIFEYIRTCSYPNNKANHLVGMAKMVTEKFG
                     GVIPDNIDDLQKLPGVGRKTANVVASVVFNKPAMAVDTHVFRVAARIGLSVNAKNPLQ
                     TEEQLVEYIPENLIPKAHHWLILHGRYVCTARKPACEKCGIAPWCNFFLNGSRKSEEG
                     "
     gene            2864..3571
                     /locus_tag="PKJ43_02930"
     CDS             2864..3571
                     /locus_tag="PKJ43_02930"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNZ71544.1"
                     /translation="MDPTVHNNGKKQLPPTTPKWISSPGNPLKGDLHRAVFTPPHLSY
                     SFNALEPIADYDTLKNHFYNFHMKGFEEFLDLINDTEVEKIGLGLLFKHSDRYPEDVI
                     EHAGCVFNHQLYWDNLSPHGGSMSEEFELVVNQNFGSVFQLKMNLFNLAQSHDCCGWL
                     WLIVTHDGKLQLTGTKHNLNPLMKGASHPGTPLLAIDMWEHAYYVKFQNNREDYLRSI
                     WMLINWNEVSRRYNFAT"
BASE COUNT          963 a          772 c          807 g         1131 t
ORIGIN      
        1 aatggctgtt tcgagctgtg aaaacatctc gttatacgaa atttcaccgc ttacgtaccg
       61 gggtatgtat ttgtattcga gcccgtaata aatgagtgct tcggggctta ccccattgtt
      121 tatcaggttt tctatttcgt cgatcattcc ttcgtcgaat cgtgctttca gtcgttctgt
      181 aatccgttgc ctgcgggttt cgcggtcgaa aagcatgcca acaatgaggg cttttatctc
      241 ggggtagtag cggtcttgct gtggggtctg ccggtaatat tcttcaattt caatggctct
      301 tgtggcccgt ttgggtgttt cgatgtcgct gttgttgtgt actgttttat attttttcag
      361 tatttcagcc agctcgtcga gggtttttcc ggccaacgat tttcgcagct cttcgtttat
      421 tggtacatta atcaatttat agccttttag tacagcttct atatacattc cgctcccgcc
      481 acaaacgatc ggaaatttgc ctcgctgacg gatgtcgttg aacgcattga tgaaatctcg
      541 ctgatattca aacacattgt attcgtatcc ggcatcggcg atgtcgatga ggtggtaagg
      601 cacaataagt ccatcgacca tataatcgct taggtctttt cctgtaccaa tgtccattcc
      661 gcggtaaacc tgcctcgaat cggccgaaat aatttcgcca ttgatttgcc tggccaagtg
      721 tgctgccacg ctggttttgc ccgaggccgt tggcccggtg atggttatca ggttgtatgg
      781 tgtagttgcc atgaaatatt tctgtgtgta aaatactttt tatgcaaatc tatttatttt
      841 tgttgccaaa attcatcaac atgagcctct ttcagccctt tactactatt tatcacgaaa
      901 gcctcgcgtt ttttttacat ccgcaggttt attggcgcga gttgaaaagc ggctcacgaa
      961 agaatgtttt tggatttaga aactttttgg tgccgggatt gataattggt tttgtgtgta
     1021 tcgtgctcgg cgatttgatt tttcactcga aaaatggttt tttttggcaa gactcgctgg
     1081 ttaaagcttg tcgtaaaacc ttgtttattt tcatgttgct tacattttcg gtaatgataa
     1141 ccaaattcgt gctcggacaa tttcacttcg aactgaaaat gaaaactgcc cggaaaattg
     1201 ccgtaatatc gttagccccg gcggtactga aaacggttat aacaggctta ctgccttttc
     1261 ttgatttggg tggggtgctg ccatggtatg gtttctttct tgcctacttt ggcttcgaaa
     1321 cgttctttgt aattcccgac gagaagaaat tttactttta ttttgcgctt tttatggcta
     1381 ttttcagtct aattatgttt ttaaccttta tattgaaccg aatttcggct cacatacttt
     1441 tataatatgg cactaaaacc acgcaccgac gtttcaataa agaacaagaa agcttttttc
     1501 gaatacgaga tcatcgaaag ctttattgct ggcattcagc tgcaaggcac cgagattaaa
     1561 tctattcgag aagggaaagc tggcctggtc gattcgtatt gccagtttgc cgggggcgag
     1621 ttgtatgtga agaacatgca tattgctgaa tatttcttcg gaaaccttcg caatcacgaa
     1681 gctaagcgcg agcggaaact cttgttgcaa aaaaaggaac tgcgaaagtt ggagcgaaag
     1741 gtaagggaaa ccggtcttac cattgtgccc ctaaagctat ttcttaacga gaaaggcttt
     1801 gccaaactcg agatcgggtt ggctcgtgga aaaaaggtgt acgataagcg cgaatcgctg
     1861 aaacaaaacg atgcccggcg cgatatggac cgggaaatga aatactaacc taggtacggc
     1921 taaccttctt cactttttct acttccattg agaaaaaagt tacaccacgg ggcgatacca
     1981 cacttttcgc atgccggttt acgagccgtg caaacgtacc ttccgtgcaa tattaaccaa
     2041 tgatgggcct tgggaattaa attctcgggg atatattcaa ctaattgttc ctcggtttgc
     2101 agtgggtttt tggcatttac cgataatcca attcgggcag ctaccctaaa tacgtgcgta
     2161 tcgacagcca ttgccggttt gttgaatacc acagaagcca ccacattcgc tgtttttcgg
     2221 ccaactccgg gcagtttctg caggtcatcg atattgtcgg gaattactcc gccaaacttt
     2281 tcggtcacca ttttggccat acccactaaa tggtttgctt tattgttggg gtatgagcag
     2341 gtgcgtatat actcaaaaat ttcttcgggc gtggccaggg ccatgaattg ggcagtaggg
     2401 tatttttcga gcaggggcgg cgtaatttgg tttacccttt tatcggtaca ttgggccgaa
     2461 aggataaccg ctaccagtag cccgaaagga gaggagtatt gcaactctgt ttcggccacg
     2521 ggcatgttca gctcaaacca ggccagtact ttctcaaaac gttcttttct gttcataacg
     2581 tgtataatga taaatttcag atattacatt cgcatcatgc aagattcatt gtgaaacagg
     2641 cctaaaatca actatgcgat ttatattcaa caacgtagaa acagcttatt ttagactgaa
     2701 aataaatgtt tcttactgaa ggtaaagtta tccattttcc tgaatttggc acatatttgc
     2761 tggatttgtg aaaatcagaa tatagaggtt ggagcgaaaa gcattgactt tgtcatcaaa
     2821 ttagaagaac ttgcatcgta aattcattct aatttaattg actatggatc ccactgttca
     2881 taacaatgga aaaaaacagt tgcctccaac aactccaaaa tggatctcca gccctggcaa
     2941 tccgttaaag ggagacctcc atcgtgcagt atttacccct ccgcacttgt cgtactcgtt
     3001 taatgcgctg gaacccatag ccgattatga tacgctcaaa aatcacttct acaactttca
     3061 catgaaagga tttgaagaat ttttggattt aattaacgac actgaggttg aaaaaatcgg
     3121 tttgggactg cttttcaaac attctgaccg ttatccggaa gacgtgattg aacatgcagg
     3181 gtgcgtattc aaccatcaac tgtactggga taacttatcg ccacacggag gaagcatgag
     3241 cgaggagttc gaattggtgg ttaaccaaaa ctttggaagc gtatttcaac tgaaaatgaa
     3301 tttgttcaat ttagcacaat cgcatgattg ttgcggatgg ctgtggctca ttgtaacaca
     3361 cgatggaaag ctccagctaa cagggaccaa gcacaacttg aatccattga tgaagggagc
     3421 ttcgcatccg ggcacaccac tactggctat cgacatgtgg gagcatgcat attatgtgaa
     3481 atttcaaaac aaccgcgaag actacctccg ttctatttgg atgctcatca actggaacga
     3541 agtgagccgc cgatacaatt ttgcaaccta agtactggca attatggtgc tcaatatatt
     3601 ttgcaagcat atctaacagc acaactgcat ctcgaacaac aaattgccgt ttgatttttc
     3661 ctgttttccc gtg
//
LOCUS       DAOPUW010000115         3671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_25588_length_3671_cov_3.978429, whole genome shotgun sequence.
ACCESSION   DAOPUW010000115 DAOPUW010000000
VERSION     DAOPUW010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25588_length_3671_cov_3.978429"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="PKJ43_02935"
     CDS             <1..797
                     /locus_tag="PKJ43_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71545.1"
                     /translation="AKAPGLASWVKTLGNLNYNNQDEWPGEAIAIISKLFLLIKTFRN
                     YDNLSPEWQITIKNLVGWSQSTKELLDDNNAERIKDFWLVAGQETETNDDIVTQRNWL
                     IGCNTQQRALILNFATRFSSFESLIVPGSIIHADLAFFPSVTPHRAVVKMQRGLETEL
                     PVLPDFFDNWTCAFQYRAKQLQQNPWANDLLVLIRDVRLLNTEKQWVAFDMENKYVPL
                     LSSFDTQKVMKWMIISGNRPQDIAFILRNGKAIPLGIFKNQQYILL"
     gene            799..2391
                     /locus_tag="PKJ43_02940"
     CDS             799..2391
                     /locus_tag="PKJ43_02940"
                     /inference="COORDINATES: protein motif:HMM:NF039502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HNZ71546.1"
                     /translation="MWQYLIETALLGTGKKNLNTENLIDDINQYSGKTFDDKEDEFLK
                     KSVLTYFYLEAGRCPSKLKAEPELSVIEEVCEIAPDELISTYLPIESLSQKLKEKLLN
                     SWLDVLVKNKWIVSPEVVLKLLELGNSFSKGIKLKIVKVIGNKGNMILPMFSGYNGVS
                     SLNSDQVWLEGSTSDRKELLDSLLEKQPERAIALLQSTWAQESVTNKRFFIELLSERK
                     SEATIQFAETLYDNEFSYREKEKKTEKECRRILAKILLSSDTSELYRFTKGKLLSYVM
                     KEKRSGLLKLLSSKSQPDYQLPENEDSEFWNGFTMEKYYGLEVQHYDIAKFNTIVQYW
                     FSELLMLMPANIWINEAFESYTELFNCLLFKDLFKIKIDGNMVPVFFNALYQNAISFA
                     DNQLSLAMIPRIPANIAIPLLKNLTQSQFEQYVQTNNFFGDEGILSNGPFSIDSNWSI
                     SFTENVLRQIFEIAMQSQYSNLSSLADVVAQLAPLDITNTLNHIEKNAVNNNLYHSWK
                     TSFYDRVVQAIEIRKSIIHYNN"
     gene            2396..3475
                     /locus_tag="PKJ43_02945"
     CDS             2396..3475
                     /locus_tag="PKJ43_02945"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ71547.1"
                     /translation="MNVLREHAEEQYREELEELKRQDSDLKPTNWNLSPQMVIKYLMG
                     GKLKNGFIVSPKYIGNQRLMEIAVATLTTDRALLLYGIPGTAKSWVSEHLAAAITGDS
                     TLLIQGTAGTSEESIRYGWNYARLLAEGPSEAAMVSTPVMKAMQTGKIVRIEELTRIS
                     ADVQDSLITILSEKTLPVPELNIEIQAIKGFNVIATANNRDKGVNELSSALKRRFNTV
                     ILPVPETIEEEVNIVQTRVASSLKSLELPASMPTLQEIQRIVRIFRELRNGVTEDGRT
                     KIKSPSSTVSTAEAISVVNSGLALAAHFGNGKLNAYDMASGLVGAIVKDPVQDAVVWK
                     EYLENVVKPREEWKDIYRACKELGN"
BASE COUNT         1160 a          708 c          798 g         1005 t
ORIGIN      
        1 atgcgaaagc tcccggatta gccagttggg taaagaccct tggtaatttg aattataaca
       61 atcaggatga atggcctggc gaggctatcg caataatatc caagttgttt ctgcttatca
      121 aaacgttccg aaactatgat aaccttagtc ctgaatggca aataaccatc aaaaacttag
      181 tcggctggag tcaatcgacc aaagagctat tggacgataa caatgctgaa agaatcaaag
      241 atttctggct cgtagccggt caggaaaccg aaacgaacga tgacattgtt acccagcgca
      301 actggttaat tggatgcaac acccaacagc gggctctgat tttaaatttt gctactcgtt
      361 tcagttcgtt cgagagcctc atcgtgcctg gaagcatcat tcatgccgat cttgcctttt
      421 tcccatcggt tacacctcat cgtgcagtgg tgaagatgca aaggggattg gaaactgaac
      481 tacctgttct tcccgatttt tttgacaact ggacctgtgc ttttcagtat agggcaaagc
      541 aactccaaca aaatccatgg gctaatgatc ttttggtctt aattcgggat gtgcggctgt
      601 taaatacaga aaaacaatgg gttgctttcg atatggagaa taaatatgtt cccttgcttt
      661 cctcattcga cactcaaaaa gtgatgaaat ggatgataat ctcaggaaat aggccacagg
      721 atattgcatt cattttgcga aacgggaaag caatcccatt aggcattttt aaaaaccaac
      781 aatatatcct tttgtaacat gtggcagtac ctaatcgaaa ccgcattgct tggcaccgga
      841 aagaaaaacc ttaacacgga aaatcttatc gatgacatta atcaatattc cggaaaaact
      901 tttgacgaca aagaggatga atttctaaaa aagtctgtct tgacctattt ttatctggaa
      961 gcaggtaggt gcccgtcaaa gttaaaagct gaaccagaac taagcgttat agaggaagta
     1021 tgtgaaattg cacccgacga gctcattagt acttatttgc caattgaaag tttaagtcag
     1081 aaattgaaag aaaaattgct gaacagttgg cttgatgtgc ttgtaaaaaa taagtggatt
     1141 gtttcacctg aggtggtgct caagttattg gaattgggaa atagcttttc caaaggaata
     1201 aaacttaaaa ttgtaaaagt tattggcaat aaaggaaata tgatcttgcc aatgttctct
     1261 ggttataatg gcgtctcatc attaaattcg gatcaagtat ggcttgaagg gtccacttcc
     1321 gatcgaaagg agttgctcga ttcccttttg gaaaaacaac cagagagggc cattgctttg
     1381 cttcaatcta cctgggcaca ggaatcagtt acgaataaac gtttttttat tgaattgctt
     1441 agtgaacgga aaagtgaggc aacaatacaa tttgctgaaa ccctttacga caatgaattt
     1501 tcttaccgtg aaaaggaaaa gaaaacagaa aaagagtgtc ggcgaatttt ggcaaaaata
     1561 ctcctttcgt cagacaccag tgaactatat cggtttacaa aaggcaaatt actttcttat
     1621 gtgatgaaag agaaacggag cggactgctc aaacttctat cgtcgaaatc tcaacccgac
     1681 tatcaattgc ccgaaaatga agacagtgaa ttctggaatg gatttacgat ggagaaatat
     1741 tatgggctgg aagttcagca ttacgacatt gcaaaattta acaccattgt tcaatactgg
     1801 ttctcagaat tactgatgct tatgcctgcg aatatttgga ttaatgaggc gttcgaatcg
     1861 tatacggaac tgtttaattg cctgctgttt aaagatctat tcaaaataaa aatagatggt
     1921 aacatggttc cagtattttt taatgcattg tatcagaatg caatttcatt tgccgacaat
     1981 cagctttcgt tggcgatgat cccacgcata cctgcaaaca ttgctattcc attgctaaag
     2041 aacctgaccc aatcccaatt tgagcaatat gtgcaaacga ataatttttt tggcgatgaa
     2101 ggaattttgt cgaacgggcc tttttcaatt gacagcaact ggtctatatc atttactgaa
     2161 aatgtgctaa ggcaaatttt tgaaattgcc atgcagtcgc aatactctaa cttatcatcg
     2221 ttggccgatg tggtggctca gcttgcccca ttggatataa cgaatacact taaccacata
     2281 gaaaagaatg ctgtaaataa caacttgtac catagttgga aaacatcatt ctacgacagg
     2341 gtggtgcaag ccattgaaat cagaaaatca atcattcact ataataatta gacctatgaa
     2401 cgttttaaga gaacacgcag aagaacagta tcgcgaagag ttggaagaac tgaaaaggca
     2461 agattcagat ttaaaaccaa ctaattggaa cctctcgcca caaatggtca tcaaatacct
     2521 gatgggagga aaacttaaaa atgggtttat tgtctctcca aaatatattg gcaaccagcg
     2581 gttaatggaa attgcagttg ccacgctaac taccgatagg gctttgttgc tttatggtat
     2641 tccgggcacg gctaaatcgt gggtctccga acatctggct gcggcaatca ctggcgactc
     2701 aaccctgcta attcagggga ctgccggcac cagcgaagag tctattcgct atggttggaa
     2761 ttatgcgcgc ttattggctg aaggccccag tgaggctgct atggtaagca ctccggtgat
     2821 gaaagccatg caaaccggaa agattgtccg catcgaggag ctgacccgta tctcggccga
     2881 tgtgcaagat tcgttgatta ctatactttc agaaaaaaca cttcccgtgc cagagctgaa
     2941 tattgagatt caagctataa aagggtttaa tgtaattgct acggccaata accgggacaa
     3001 aggggttaat gagctctctt cggctttaaa acgaagattc aataccgtaa ttctccctgt
     3061 tcccgaaacc attgaagagg aggttaacat tgtgcaaacc cgtgttgcca gttcattgaa
     3121 gtcactcgaa ttgcctgctt ctatgccaac actgcaagaa attcagcgaa ttgtaagaat
     3181 ttttcgggag ttgcgcaacg gggtcaccga agatggtcgc actaaaataa aatcgccttc
     3241 aagtacagta agtactgctg aagccatttc ggtggtgaat agtggcctgg ctttggctgc
     3301 ccattttggc aatggtaaat taaatgccta cgatatggca tcaggtctgg ttggtgccat
     3361 cgtaaaagac cctgtacaag atgcggtggt ttggaaagag tatctcgaaa atgtggttaa
     3421 gccccgcgaa gagtggaaag acatttaccg ggcatgcaaa gagttgggaa actaaggttt
     3481 tttggaaaac aaacagctaa taactacatc tatggacgag aactacaaaa gccatattct
     3541 tcaatattct caagacaaga aggattatat acagagaaac agatacgcag gtgaggaaca
     3601 attgcttcct tttcttgata aagtgaccaa tgtgctgatt ggagatgagc cctttagtga
     3661 cgatgttctg g
//
LOCUS       DAOPUW010000116         3654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_25745_length_3654_cov_4.518755, whole genome shotgun sequence.
ACCESSION   DAOPUW010000116 DAOPUW010000000
VERSION     DAOPUW010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25745_length_3654_cov_4.518755"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="PKJ43_02950"
     CDS             <1..988
                     /locus_tag="PKJ43_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ71548.1"
                     /translation="SYTFTIAGNRNLVANFVAQTPTTYTVALSASPSNGGTVSGGGTF
                     NEGINVTAHAAPMAGYTFVNWTENGAIVSTSVSYIFQVTSNRTLIANFQLVAAEEYTV
                     GLSVAPVASGTVSGSGTFESGSSVTVSANAANGYNFLNWTEGDNVVSSNATYTFTITS
                     DRDLKANFEPEKYTITLTANPSAGGFTNFSSNQFAFGQSVTINANENSNYKFVNWTEN
                     GVEVSTNKSYTFMAEANRSLVANFIPQTGVEDIEELKMSVYPNPTTGIVHVSGIQEGM
                     ELNITDVSGRLVHSVKADNTTVILDLSSFRHGIYFIRVVDKQFHYTKRVVLR"
     gene            1218..3071
                     /gene="ispG"
                     /locus_tag="PKJ43_02955"
     CDS             1218..3071
                     /gene="ispG"
                     /locus_tag="PKJ43_02955"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HNZ71549.1"
                     /translation="MMDRNFNYCKTLTEYHREETMEVNIGGVPLGGFNPIRLQSMTDT
                     DTMDSDATVDQFIRIVKAGADYVRIAVKTTKDAENLKEIKAKIRALGFQTPIIADVHF
                     NPKIAEIAARYVSKVRINPGNFYDKRAQFEKLIYTDEEYRQELIKLEETLTPFINICK
                     ENDTAIRVGANQGSLSDRIMSRYGDTPAGIVESVMEFLRICKKNDFHKIVISIKSWNT
                     RTLVYTVRLMNYKMRLEDMNYPFHIGVTEAGEGEDGRIRSAVGIGTLLIDGIGDTIRV
                     SLTEKPENEIPFAKKLVDVITSRRGHQKIGAPGFAQTNPFEFERRYGRPVRKIGNRRA
                     PVVVADLNGNEILEVSNLNGKLEPDYYYDGRHIINRLNKRFPILSLEDYLYNDIYQSS
                     HRFIRMSRKEFVEFRNENPEIVQKLKAERKAVILLESENSNRYADMRAFFMIMESIVC
                     RLSVILCGQYREKNLLDLQLKAATDFGGLFIDGYGDGILLENEGHDIFYSDLKNTSFA
                     ILQATRMRITKSEFISCPGCGRTQFDLHTTTLEIKSHFSHLKHLKIGIMGCVVNGPGE
                     MGDADYGFVGAGNGKINLYKGQKVVKRHIPHEEAVAELEKIIRENNDWVED"
     gene            complement(3108..>3654)
                     /locus_tag="PKJ43_02960"
     CDS             complement(3108..>3654)
                     /locus_tag="PKJ43_02960"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNZ71550.1"
                     /translation="SEEADVSRPISPYAFTKKACELINYTYHHLYQFDIINMRFFTVF
                     GPRQRPDLAIHKFTKLIRNHQPINMFGDGTTARDYTFIEDTLQGICASIDYLLTHENV
                     FETINLGNNQPVTLSDMIDAIANATGEKPVINQLAMQPGDVDITLADISKANKLLHYQ
                     PAFNFNEGVKKFVEWYDEVNR"
BASE COUNT         1050 a          707 c          810 g         1087 t
ORIGIN      
        1 aagctatacg tttactattg ccggcaaccg taacctggtg gcaaacttcg tagcgcaaac
       61 cccaactacc tacaccgttg ccttatcagc ttctccgtca aatggaggaa cagtcagtgg
      121 cgggggtaca tttaacgaag gaattaatgt tacagctcac gctgcaccta tggctggcta
      181 cacgtttgtg aattggacag aaaacggagc gattgtttca acttctgtta gttatatttt
      241 tcaggtaact tcaaatagaa ctctgatagc aaatttccag cttgttgctg cagaagagta
      301 tacagttgga ttgtcggttg ctccagtagc atcgggtact gttagtggtt ccggcacttt
      361 tgagtcgggt agctcggtta ctgtttctgc caatgctgca aatgggtata attttcttaa
      421 ctggactgaa ggggataatg ttgtttcgtc aaacgcaaca tatacattca caattacttc
      481 tgatcgggat ttgaaagcaa attttgaacc tgaaaaatac acaattactc ttactgcaaa
      541 tccatcggca ggaggcttta ctaatttttc aagcaatcaa tttgcttttg gacaaagtgt
      601 taccattaat gcaaatgaaa acagcaatta taaatttgtt aactggacag aaaatggggt
      661 tgaagtatca acaaacaaat cgtacacttt tatggcagaa gcaaatcgtt ctttggttgc
      721 caattttatc cctcaaaccg gcgttgagga tattgaagaa ctaaagatgt cggtttatcc
      781 gaaccctaca acaggtattg tgcatgtttc tggaattcaa gaaggtatgg agcttaatat
      841 aaccgatgtt tcgggtcgat tagtccattc tgtaaaagcc gataatacta ccgtaattct
      901 cgatttgtca agctttaggc atggaattta cttcatcagg gttgttgata agcaatttca
      961 ttacactaaa cgggttgttc tgcgttagtt ttactagggt taataattaa gcaaagtata
     1021 aaaagataga aaatcggggc atgcttgatt cataagcatg ccccggcttt tttttgttct
     1081 tgcaatttct gaaaaagtct taattgaagc ttttgttcca ttttttgttc cattttttgt
     1141 ttttctgtca caattactgt ctttatatta tggtaaagtt ttaaatttgc tttttcatat
     1201 taaagaggaa acaggctatg atggatcgca attttaatta ctgcaaaacc cttaccgaat
     1261 atcacagaga agaaacgatg gaggtgaata ttggtggcgt tccgttgggc ggcttcaacc
     1321 cgatccgatt gcagtcgatg accgacaccg acacaatgga cagcgatgcc actgtcgatc
     1381 agtttattcg cattgttaaa gctggtgccg actatgttcg tattgcagta aaaacaacca
     1441 aagatgccga aaacctcaaa gaaattaaag caaaaattcg cgccctgggt ttccaaaccc
     1501 ctataattgc cgatgtgcat ttcaatccta aaattgcaga gattgctgcc cggtatgtgt
     1561 ctaaagtgcg tatcaatcct ggtaacttct acgataaaag agctcagttt gaaaaactta
     1621 tttataccga tgaagaatat cgtcaggagt tgattaagct ggaagaaacc ctaactcctt
     1681 ttattaatat ctgtaaagaa aacgatacgg ctattcgcgt tggggctaat cagggctcac
     1741 tttctgaccg tattatgagc cgctatggcg acactccggc aggaatcgtt gagtcggtga
     1801 tggaattttt gcggatctgt aaaaagaacg atttccataa aatagttatt tctatcaagt
     1861 cctggaatac aagaactttg gtgtatactg tacggttgat gaattacaag atgcgcctcg
     1921 aggatatgaa ttatccgttc catattgggg ttaccgaggc gggcgaaggt gaagacgggc
     1981 gtatcagatc ggccgttgga attggcacct tgctcatcga tggaattggc gacacgataa
     2041 gggtttcgtt gactgaaaaa cctgaaaacg aaattccttt tgccaaaaaa cttgtcgatg
     2101 taattaccag ccgccgtgga caccaaaaga ttggtgcccc ggggtttgct caaactaatc
     2161 cgtttgaatt tgaacgccgt tacggacgac cagttcgcaa aatcggaaac cggcgtgctc
     2221 cggttgtagt cgctgacctt aatggaaatg aaattctcga ggtctcgaac cttaacggga
     2281 aacttgagcc tgactattat tacgatgggc gccatattat caatcgcttg aataaaagat
     2341 tcccgatcct ttcgcttgaa gattatttat acaacgatat ttatcaaagc agccaccggt
     2401 ttatccgaat gtcgcgaaaa gaatttgtag agttcagaaa cgaaaacccg gagatcgtgc
     2461 aaaagcttaa ggctgagcga aaagctgtga tccttctcga atctgaaaac tcgaaccggt
     2521 atgccgatat gagggctttc ttcatgatta tggagtcgat tgtttgccgc ttatcggtta
     2581 tcctttgcgg acaataccgg gaaaaaaatc tgctcgattt gcaattgaaa gctgcaaccg
     2641 actttggagg tcttttcatt gatggttacg gcgacggaat cttgctcgaa aacgaaggcc
     2701 atgatatttt ctactctgat ttgaagaata ccagttttgc aatcttgcag gctacccgta
     2761 tgcgtattac taaatcggaa tttatttcat gccctggttg cggccgtact cagttcgatt
     2821 tacataccac tacgctcgaa ataaaatcgc atttttcgca ccttaaacat ctgaaaattg
     2881 ggataatggg ttgcgttgta aatggtccgg gcgaaatggg tgatgccgat tacggttttg
     2941 ttggagctgg caacggaaaa attaacctgt acaaaggaca aaaagtggtt aagcgccata
     3001 ttcctcacga agaagctgta gctgaactcg aaaagattat ccgcgaaaac aacgattggg
     3061 ttgaggatta attctgaaaa ttattcaaag ggcgattgaa ttttccttta cctgtttact
     3121 tcatcatacc actcaacaaa cttcttaact ccttcgttga agttgaatgc aggttgataa
     3181 tgaagtagtt tgtttgcctt cgatatatct gcaagcgtaa tatctacgtc gccgggttgc
     3241 atcgccagtt ggttgattac cggtttctcg ccggttgcat tggcaatagc atcaatcata
     3301 tcactgagtg taacaggttg gttattccct aaattgatgg tttcgaacac gttttcgtga
     3361 gtaagcaaat aatcaatcga tgcacatatt ccctgcaaag tatcttcgat aaacgtgtag
     3421 tcgcgtgcag tcgtcccgtc gccaaacata ttgattggtt ggtggttgcg tatgagtttt
     3481 gtgaatttat gaatggcaag atcaggtcgt tggcgcggac caaacaccgt gaagaaccgc
     3541 atgttaataa tgtcgaactg atataggtga tgataggtgt aatttatcag ttcacaggct
     3601 tttttagtga aagcgtaggg cgaaattggc ctcgaaacgt cagcttcttc agaa
//
LOCUS       DAOPUW010000117         3654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_25748_length_3654_cov_3.198666, whole genome shotgun sequence.
ACCESSION   DAOPUW010000117 DAOPUW010000000
VERSION     DAOPUW010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25748_length_3654_cov_3.198666"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..428)
                     /locus_tag="PKJ43_02965"
     CDS             complement(78..428)
                     /locus_tag="PKJ43_02965"
                     /inference="COORDINATES: protein motif:HMM:NF038084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HNZ71551.1"
                     /translation="MNIPFQIIDWSNIPRSEHKGESGLAYWQTIQVGGLRIRLVEYSK
                     GYIADHWCTKGHIVHCLEGDFVSELLDGEKFTLTRGMTYVVSDELSSHRSSTEHGVKL
                     LIIDGDFLKLADDE"
     gene            complement(516..>1585)
                     /gene="rny"
                     /locus_tag="PKJ43_02970"
     CDS             complement(516..>1585)
                     /gene="rny"
                     /locus_tag="PKJ43_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNZ71552.1"
                     /translation="DAKAEAMSYIQEIMDEAKLNANKEAKKIIVKSIQRVASETAIEN
                     AVTIFNIENDEIKGRIIGREGRNIRALEAATGVEIIVDDTPEAIVLSAFDPVRREIAR
                     LALHQLVTDGRIHPARIEEVVNKVSKQIEEEIIETGKRTCIDLGIHGMHPELVRLVGK
                     MKYRSSYGQNLLQHSRETANLCAIMAAELGLNPKKARRAGLLHDIGKVPDEEPELPHA
                     VLGMKLAEKYKEKPDICNAIGSHHEEVEMQTLLAPIVQVCDAISGARPGARREAVESY
                     IKRLNELESMAMSYNGVTKTYAIQAGRELRVIVGSDKISDAESEQLSYDIAKRIQDEM
                     TYPGQVKITVIRETRAVSYAK"
     assembly_gap    1586..1685
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1686..2221)
                     /locus_tag="PKJ43_02975"
     CDS             complement(<1686..2221)
                     /locus_tag="PKJ43_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HNZ71553.1"
                     /translation="MNWIYTIVAFALGLPITYFIWDKALHRKREKMLKEAENEAEVLK
                     KDKVLQAKEKFLQLKSEHEKYINERNNKLLANENRVKQKETEYLRRKDELSRSIRDFE
                     VEKKETEVIRENLTHQLELVEQKSEEVEKLHRQEVEKLEIISGFSADEAKAQLVESLK
                     NDAKAEAMSYIQEIMDEA"
     gene            complement(2322..2627)
                     /locus_tag="PKJ43_02980"
     CDS             complement(2322..2627)
                     /locus_tag="PKJ43_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010424497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNZ71554.1"
                     /translation="MDEELSINVTVADRRYPMRIRRSDEEKIRKAAKIINERILQYQQ
                     RYSGKDNQDFLAMSALQFVMQIMENVEQTNISPFVKQIEEINEQLALYIDKNQRSNE"
     gene            complement(2637..2930)
                     /locus_tag="PKJ43_02985"
     CDS             complement(2637..2930)
                     /locus_tag="PKJ43_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71555.1"
                     /translation="MTEKDQHLIEELRMNIRRLMESLDASKSELIAVKEECRNLEERF
                     VQLSSENEELKKRYENLKVAKVLAEGDPDTQAAKQKITKLIREVDKCIALLNQ"
     gene            3173..>3654
                     /locus_tag="PKJ43_02990"
     CDS             3173..>3654
                     /locus_tag="PKJ43_02990"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNZ71556.1"
                     /translation="MKFLFISICTLFLVIEGFCQTPSFVSPVNIPLVLNGNFGEIRSN
                     HFHAGIDIKTNGATGLPVFSISDGVVSRISVSATGYGKTLYIDHPSGYTSVYAHLDRF
                     IPEIEKWVKEQQYQLQRFEVNLEPSPTLFRFSKGEQIAFSGNTGSSAGPHLHFEIRKT
                     "
BASE COUNT          899 a          834 c          659 g         1162 t
ORIGIN      
        1 tcctgctcag ttgggaaacc tttttttcat taaaaacggt caaaagggta atgaccagta
       61 tttgctatta tatcctttta ttcatcatct gccaacttca aaaaatctcc gtcaataatc
      121 aataacttaa ctccatgctc tgttgacgag cggtgtgaac tcaactcatc agatacaaca
      181 taagtcattc ctctggtcaa tgtaaatttt tcgccgtcaa gaagttcact aacaaaatcg
      241 ccttccaaac aatgcacgat atgacctttt gtacaccagt gatctgcaat ataacctttt
      301 gaatattcaa ccaaccgtat tcttaatcct ccaacctgaa ttgtttgcca ataagcaagc
      361 cccgactcgc ccttatgctc agaccttgga atgtttgacc aatcaattat ctgaaatgga
      421 atgttcattt tatccatgtt atatccttct tttattaaat aaaaacgggg cactcccaaa
      481 agagcccccc gttacaatat catgttattt attaattact tagcataact cacagcacga
      541 gtttctcgaa taacggtaat tttcacctga ccaggataag tcatttcatc ctgaatccgt
      601 ttagcgatat cgtaagacag ctgttcggat tcggcatcag aaatcttgtc gctgccaaca
      661 attacacgaa gttcgcggcc tgcctgaatt gcgtaggttt tagttacacc gttgtacgac
      721 atagccatgc tttccaattc gttcaatcgt ttgatatacg actcaacagc ttcgcgacgt
      781 gcacccggac gtgcaccaga gatagcgtcg caaacctgaa cgataggagc aagcagggtt
      841 tgcatctcca cttcttcatg gtgcgaacca atggcattgc agatatctgg cttttctttg
      901 tacttctcgg ccaacttcat accaagcact gcgtgcggta attcgggttc ctcgtcgggc
      961 accttgccta tgtcgtgcaa aagccctgcg cggcgtgctt ttttgggatt cagcccaagt
     1021 tcggcagcca taatggcaca aaggtttgct gtttcacgtg agtgctgcag caggttctga
     1081 ccatacgatg aacggtattt cattttcccc acaagacgca ccaattcggg atgcatacca
     1141 tgtattccaa ggtcgatgca agtgcgtttt ccggtttcaa taatctcttc ttcaatttgt
     1201 ttgctcactt tattaaccac ttcctcaata cgggcagggt gaatacgccc gtcggtaacc
     1261 agttggtgaa gggcaaggcg ggcaatttcg cggcgtacag gatcaaatgc tgaaagcacg
     1321 attgcctccg gggtatcgtc aacaataatt tcaactcctg tagctgcctc aagggccctg
     1381 atgttacgac cttcacgacc aataatccgg cctttaattt cgtcgttttc gatattaaag
     1441 atggtaactg cattctcgat agcagtttca gaagcaacgc gttgaataga ttttacaatg
     1501 attttcttgg cttctttatt ggcattgagc ttagcttcgt ccatgatttc ctgaatatag
     1561 gacatggcct cggctttggc atcgtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnttagc ttcgtccatg atttcctgaa tataggacat ggcctcggct ttggcatcgt
     1741 ttttcaaaga ttcgactaac tgggctttgg cttcatcggc cgaaaaacca gagattattt
     1801 ccagcttctc aacttcctga cggtgaagtt tttctacctc ttcgcttttt tgttctacca
     1861 attcgagctg gtgagtaaga ttttcgcgga tcacttcggt ttctttcttt tcaacctcga
     1921 agtctctgat gctgcgcgaa agttcatctt tacggcgaag gtattcggtt tctttttgtt
     1981 ttacacggtt ttcgttggcc aacaacttgt tgttccgttc gttaatatac ttttcgtgtt
     2041 ccgattttaa ctgcaggaat ttctcttttg cctgcaaaac tttatccttt ttaagtactt
     2101 cggcttcatt ctcggcctct ttcagcatct tttctctttt ccgatgcaac gccttgtccc
     2161 atataaaata ggttattggg aggcccaacg caaatgcaac tattgtatat atccaattca
     2221 tcaattaatt ttttattatt agttagtaaa aaaaaacgca ttccaacact gtgaatatga
     2281 actcgatatt caaacatgct gagatgcggc gattatatat tttattcgtt agaacgctgg
     2341 tttttatcga tataaagagc taattgttcg tttatctctt caatttgttt tacaaaagga
     2401 ctgatgttgg tttgctcaac attttccata atttgcatta caaactgcag ggccgacatg
     2461 gccagaaaat cctggttgtc ttttcctgaa tatcgttgtt gatattgtaa aatccgctca
     2521 tttattattt tcgcggcctt acgtatcttt tcttcgtcgc tacgccttat cctcatcgga
     2581 tatcgcctgt cggcaaccgt aacatttatc gatagttctt catccatagt gacccgctat
     2641 tgatttaaaa gggcaataca tttatcgact tcgcgaatca attttgtaat cttctgtttt
     2701 gccgcctgtg tatcaggatc gccttcggct aaaacttttg ccacttttaa attttcatac
     2761 ctttttttga gctcttcatt ctcgctactg agctgaacaa atcgttcttc aaggttacga
     2821 cattcttcct tgacagctat caattcgctc ttcgaagcat cgaggctttc cattaaccgc
     2881 ctgatgttca ttcgcaattc ctcaatcagg tgctgatctt tctcagtcat ttttgaatga
     2941 tttctgtaca aaaataacat ttttaataaa cggaaaatac atgtagttcc cgataccgaa
     3001 gtttttttgt catctgacca taatatctgc ctaaaatgct gtaaaaatgt aaccctgcac
     3061 aacatctgtt ctcgacaatt aaataactgt caattttgcc ggctacaaac cgcacgatcc
     3121 tgttattcgg tgcagaaact gtattttagc ctcatgttag tctagatggg ttatgaagtt
     3181 tctttttatt tctatttgca cactgtttct tgttattgag ggattttgtc aaacaccctc
     3241 gtttgtttcc ccggtgaata ttccgctcgt gctaaacggt aatttcggcg aaatcaggtc
     3301 gaatcacttt cacgcgggca tcgacataaa aaccaacgga gctacaggcc tgccggtttt
     3361 ttctatcagc gacggggttg tttcacgcat ttcggtttcg gcaaccggct acggaaaaac
     3421 tctttatatc gatcatcctt caggctatac ctctgtatat gcccatctcg accgttttat
     3481 tcccgaaatt gaaaaatggg taaaagaaca gcagtaccag cttcagcgct tcgaagtaaa
     3541 ccttgaaccc tcgcccactc ttttccgttt tagcaaaggc gaacaaatag ctttttcggg
     3601 caacaccggt agctcggccg ggccacatct ccatttcgaa atcaggaaaa ccga
//
LOCUS       DAOPUW010000118         3587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_26428_length_3587_cov_3.298981, whole genome shotgun sequence.
ACCESSION   DAOPUW010000118 DAOPUW010000000
VERSION     DAOPUW010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26428_length_3587_cov_3.298981"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PKJ43_02995"
     CDS             complement(<1..251)
                     /locus_tag="PKJ43_02995"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ71557.1"
                     /translation="MMKCFLIQSNQPPVQKNYNFFLTGATGILGSHVLFELLLKYIKK
                     ELVGKIVLLVRPDKKNTARQRILSVLTSSAIPLWLKKIP"
     gene            complement(304..1059)
                     /locus_tag="PKJ43_03000"
     CDS             complement(304..1059)
                     /locus_tag="PKJ43_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71558.1"
                     /translation="MGKIMLVIAFFLMGSIYMLNIEKESTSGVLFNNPAESHGVAYYK
                     TTEPREGAFSFLVPANWEISGGITRVEQEPKLYLKLSSPDNKTTVCWLPEMEFIVPEN
                     RSRNIGNTVDEQLKIKSLLTPADFVLTIAVPFAHPHAATIRVTDVKPITPVWMFSLSG
                     EMNAANRQAAEVTISYWENGVFFKEKITCVIESYTENGDKKWRNFSTWYVRCDSNSFE
                     AFEPIFSKIISSVEPDKAWTEREIQRRQIVSPR"
     gene            complement(1146..>3587)
                     /locus_tag="PKJ43_03005"
     CDS             complement(1146..>3587)
                     /locus_tag="PKJ43_03005"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HNZ71559.1"
                     /translation="WNMHDNVAQDRYIDSSLYAFNGATASEYCYLFTPPYKGEFLDII
                     IAEIREKIIGWYDFYQFDKPVNTEYEVLRHDTAIVYSALGMNIDVDIITRVVGKRNAD
                     ENVETGLGTFNAKKFSYNIIITAYLHIFGDVAEIVFADFPVIHWITSDKWIVKEWRPT
                     VITTDALEFVGINSITAPGLDRTLTGYTAPKPVTPTLNLPQNNSSNVSKEPTLQWNQS
                     NLADIYFLQIATDMGFTNVVYEDSTISANSKEISGLSAGTNYYWKVKAKNSNGISNWS
                     DTWNFSTEEDKFLNVDKTNVSVSASEGSTTSLAISSNTEWTVASDQTWLTINPLSATG
                     NKTISLTANKNNTINPRSATISIASQGLPTKTVTVTQDAGAATLDVSSSNLTIESTNG
                     STTNFSVTSNTSWNASSDQSWLSLNPPSGNGNLTVVATAEANLTTSSRSGTIFLSAPG
                     TTSKSISVTQAAGAATLKVSTTSINIGAMANSSSSVTITSNTSWDISSDQPWLEVSPE
                     NGVGNQLVTLVAQENPTILNRTATITISPNGTASKTITVTQASGSETLLLSTSAITFE
                     ATEGSSSVVNVISNTGWSVSVNEEWLTATPSTGSGNGSLTIVTTENSSTIERTATVTV
                     TIEGTETKQIVAVTQKGASPMLVVSDYSLTIGALEGSTAQFTIASNTSWNISSTSNWL
                     STNIVAGESDQTVVLTATKNPSSSERTATIIVTAEGVAQKTLTITQSIFTASERLFPK
                     NVKIYPNPFSEGIYIDSSSDNAHLLIADINGHIVFEAPVSGVTYIPLENLRNGIYHIC
                     FIENGQSHRKTLIKK"
BASE COUNT          945 a          707 c          777 g         1158 t
ORIGIN      
        1 atgggtattt tcttcaacca taaaggaatg gcagaactgg taagaacgga aagtattcgc
       61 tgacgtgcag tatttttttt gtcggggcgt accaatagta caattttacc cactaactct
      121 ttttttatat acttcaacaa aagttcaaaa agcacatgtg accccagtat accggtagcc
      181 ccggtaagaa agaaattata gtttttctgc actggtggtt ggttcgattg aattaaaaag
      241 catttcatca ttttattttt ttcgggttat catttatttc cattgaaatg aaatgagccg
      301 ggattaacgt ggtgacacaa tctgccggcg ttgaatttca cgttctgtcc atgctttgtc
      361 gggttcaaca gacgatatga tttttgaaaa gattggttcg aatgcttcaa agctattgct
      421 atcgcacctt acataccatg tggaaaaatt tctccatttt ttgtccccgt tttctgtata
      481 ggattcgatg acgcatgtta ttttttcttt gaaaaacact ccattttccc aatacgatat
      541 ggtgacttca gctgcctgtc tgttggccgc attcatttca cccgacaggg aaaacatcca
      601 gactggggtg atgggcttaa catcggttac cctaatggtt gcagcatgag gatgggcaaa
      661 aggaacggca atggtaagta caaagtcggc gggtgtcaat aaactcttta ttttcagttg
      721 ttcgtctacg gtgttgccga tattccgaga acggttttcg ggaacaataa actccatttc
      781 gggcaaccaa caaactgtgg ttttattgtc gggtgagctt aacttcaagt aaagttttgg
      841 ctcttgttca acacgtgtta ttcctcccga tatttcccaa ttggcaggta ccaggaaaga
      901 aaaagctcct tccctgggct ctgtggtttt ataataagca accccatgac tttctgccgg
      961 attattgaaa agtaccccgc tggtgctctc tttttcaatg ttcagcatgt atattgatcc
     1021 cattaaaaaa aaggcaatca ccaacattat ctttcccata ctcataaatc tttgtttcct
     1081 aaaatgttgc ctgaataatc tatgaattgg ccggagaatt tactctccgg ccaagactag
     1141 gacagttact tttttataag ggttttacgg tgcgattgcc cgttttcaat aaagcaaata
     1201 tgatatatcc cgttccttag gttttcgagc ggaatgtatg ttacgcctga aaccggtgct
     1261 tcaaacacaa tgtgaccgtt tatatcagca atcaggagat gggcattgtc ggaagatgaa
     1321 tcgatataaa taccctcgct aaacggattg gggtaaatct taacattctt cgggaataat
     1381 ctctcactgg ctgtaaaaat ggattgagta atggtcaatg ttttctgggc tactccttct
     1441 gctgtgacaa taattgtcgc agtccgttct gacgaagatg gatttttggt tgcagtcaat
     1501 actacagtct gatcactctc tcctgcaaca atatttgtgc taagccagtt acttgttgaa
     1561 gaaatattcc aggaagtgtt tgatgcaatg gtaaactggg ccgtgctccc ctccaatgcg
     1621 cctatggtta atgaataatc ggaaacgacc aacattggcg atgcgccttt ttgagtaaca
     1681 gctactattt gtttagtttc cgtgccctca attgtaaccg taactgtggc tgttcgttca
     1741 atcgtgctac tgttttctgt tgtcacaatg gtaagtgaac cattaccact accggttgag
     1801 ggggttgcag taagccattc ttcattaaca gagacagacc aaccggtgtt tgaaatgaca
     1861 tttaccacgc ttgaactgcc ttctgttgct tcaaaggtta ttgcagatgt tgaaagtaaa
     1921 agagtctcac tgcccgaagc ctgggtgaca gtgattgttt tagaagcagt gccgttaggt
     1981 gaaatcgtaa tggttgcggt tcggttcaat attgttgggt tctcttgggc tacaagggtt
     2041 accagttggt ttccaactcc gttttcaggg cttacttcca gccatggttg atcggatgaa
     2101 atatcccaac ttgtatttga agtaatggta accgacgatg agctgttggc catagcccca
     2161 atgtttattg atgtagtgga gactttaagt gttgctgctc cagcagcctg tgtgactgaa
     2221 atactttttg atgtagtacc tggtgccgat agaaaaattg ttccactccg gcttgaagtt
     2281 gtcagattgg cttcggctgt ggcaacaact gtaagatttc cgtttccaga tggaggattt
     2341 aggcttagcc acgattgatc ggaactggca ttccatgaag tatttgatgt aaccgaaaaa
     2401 ttagttgtgc tcccgtttgt tgattcaata gtcaggttac ttgatgaaac atctaaggtt
     2461 gcagcccctg cgtcctgggt tacagtaact gtttttgtgg gcagtccttg ggaagcaata
     2521 gaaattgttg cagaacgggg attaatcgtg ttgtttttgt tggctgtcag gctgatggtt
     2581 ttattgcctg tcgccgagag tggatttatg gtgagccatg tctggtctga ggcgactgtc
     2641 cattcggtat tcgaggagat agccaatgat gttgtgcttc cttccgaagc agaaacactt
     2701 acattagttt tgtctacatt caaaaattta tcttcctcgg tagaaaaatt ccatgtatcg
     2761 gaccagttgc ttataccgtt gctgtttttt gctttgacct tccagtaata atttgtccct
     2821 gcgctcaacc cgcttatctc ctttgaattt gctgaaatcg tggaatcttc ataaaccaca
     2881 ttggtaaagc ccatgtcggt tgcaatctga agaaaataaa tatctgccaa gttcgattgg
     2941 ttccattgaa gtgttggttc tttggaaaca tttgaactat tgttttgggg cagattaagt
     3001 gtaggtgtaa ctggttttgg cgcagtatat cctgttaaag ttctgtcgag tcctggtgca
     3061 gtgattgaat tgattccaac aaactcaagt gcatcggttg tgatcacagt tggcctccat
     3121 tcctttacaa tccatttatc acttgtgatc cagtgaataa caggaaaatc agcaaaaaca
     3181 atttctgcaa catcgccaaa aatgtgcaaa taagctgtta tgataatatt ataggaaaat
     3241 tttttagcat tgaaagttcc caatccggtt tctacatttt catcggcatt tctttttcca
     3301 acaacgcgag taataatatc aacatcgata ttcatgccca gtgctgaata gacaattgcc
     3361 gtatcgtgcc ttaggacttc atattcggta ttaactggtt tgtcgaattg ataaaagtcg
     3421 taccagccga taatcttttc tctgatttca gcgattataa tatccagaaa ttctccttta
     3481 tagggaggtg taaacagata acaatattcc gaagcagtag cgccgttgaa tgcatacagc
     3541 gaagaatcaa tgtatctgtc ttgggcaaca ttgtcatgca tattcca
//
LOCUS       DAOPUW010000119         3582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_26474_length_3582_cov_3.351857, whole genome shotgun sequence.
ACCESSION   DAOPUW010000119 DAOPUW010000000
VERSION     DAOPUW010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26474_length_3582_cov_3.351857"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PKJ43_03010"
     CDS             complement(<1..938)
                     /locus_tag="PKJ43_03010"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HNZ71560.1"
                     /translation="MKQVYIFLFVSMFSLYAWSVNLPNPVVDAIYSDQFKTVQFHPVG
                     KSLEPPVVFFNNMQQLCLSFDDLSNEVQNLYYTVFHCDRNWKISAIPQEEYLLNFTDF
                     VLDDYAFSKNTLVKYVNYQLLLPNDDVDIKYSGNWAVVVFNKDHPDEPLIVKRFFVVE
                     PVVGLDARIRDNRFDVSGGAKQEIDFILSKNGFQVRNPRTDLKVVVSQNFRFDNAVSN
                     LNPLFDDEKKLEYGLNSEINFTGDNEYRFFEFREIEFPGRGVESIKLHRPLYHITLKP
                     DKIRRGERYSSDREINGLYRVEMYRSDDPEVEADYM"
     gene            1216..2559
                     /locus_tag="PKJ43_03015"
     CDS             1216..2559
                     /locus_tag="PKJ43_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HNZ71561.1"
                     /translation="MSNTIRIKKGLDIKLQGKAELVVETPLSSATFAIKPSDFKGLVP
                     KLSVKADDIVKAGTPIIHDKYNPEIQFVSPVSGKVVNINRGDHRMILEVIIESDNKND
                     SIEFNVSGWKSAGREEIIKQLLDSGLWPYIIQRPFGLIAKPSDTPRDIFVSGFDSAPL
                     APDFDFIVGTETAAFQTGLEVLSKLTSGKVFLGLRNGSKLSSMKGVEINYFEGPHPAG
                     NVGIQIHHLKPIDKGINVWTLSPQAVLFIGRLFTNGKLDFSETIAITGSEVKSPCYLK
                     TISGAEINSLVAGRTKKEVNERIISGNVLTGVKSDSNGYLGFYHNQITVIPEGDNYEF
                     MGWAMPRFNKFSVSRAYFSWLNPGKKYRADANLNGEERAYVMTGQYEKVLPMEILPVH
                     LIKAIIAEDFDKMEQLGIYEVIEEDMALCEYVCTSKIEVQQILRKGISMVVKELG"
     gene            2584..>3582
                     /locus_tag="PKJ43_03020"
     CDS             2584..>3582
                     /locus_tag="PKJ43_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HNZ71562.1"
                     /translation="MNSLRKYIDKNKPLFQKGGKFEKLGSTFGAFETFLFTPNHTAKS
                     GSHIHDVIDLKRTMIVVVLALLPALLFGCWNVGYQNGLAFGIERSLLGHFFFGFWKFL
                     PLFIVSYVVGLGIEFASAQIRGHEVNEGYLVTGILIPLIIPINTPLWMLALAVAFAVI
                     FGKEIFGGTGYNIMNVALIARAFLFFAYPSEMSGDKVWIYGLPDQAAIDGFSGATPLA
                     QLAQGATQNMASMSDLFMGFIPGSIGETSKLAILIGAAILLFTGIASWKIMLSTVIGV
                     LSIGLLCNAFALNEYMAVPAYYHLLIGGFAFGAVFMATDPVTAPHTEKGKWIYGFLI"
BASE COUNT         1144 a          753 c          707 g          978 t
ORIGIN      
        1 atcatataat cagcctcaac ctcagggtcg tcagaacgat acatttcaac ccggtataaa
       61 ccattaattt cacgatcaga cgaatagcgt tcaccacgcc ttattttatc gggtttcagc
      121 gttatatgat ataatggccg atgaagtttt attgactcaa cacccctgcc cggaaattca
      181 atttcccgaa actcgaagaa tcgatattca ttatcgccag taaaattaat ctccgaatta
      241 agaccatatt caagtttttt ctcatcgtcg aataagggat ttaagttaga aacagcattg
      301 tcgaaacgaa aattctgtga aacaacaact ttcaaatcgg tacgaggatt gcgaacctga
      361 aaaccattct tagataggat aaaatcaatt tcctgcttgg cgccccctga aacatcaaag
      421 cggttatctc gaattcgtgc atcgagacca acaacaggtt ccaccacaaa aaaccttttc
      481 acaataagag gctcgtcagg atgatcttta ttgaaaacaa ctacggccca attccccgaa
      541 tattttatat ctacatcgtc attaggtaaa agaagctgat aattgacata ttttaccaat
      601 gtatttttag aaaacgcata atcatcaagc acaaagtcgg taaaattcag cagatattct
      661 tcttggggta ttgcagaaat tttccagttt ctatcgcaat gaaaaactgt ataatacaga
      721 ttttgaacct cattagacaa gtcatcgaac gacaaacata gctgctgcat gttgttgaaa
      781 aaaactaccg gaggctcaag gcttttgcct acgggatgaa attgaacagt cttaaactga
      841 tcggaatata tggcatcgac aacagggtta ggaagattca ccgaccatgc atacaacgaa
      901 aacattgaca caaacagaaa aatatatact tgtttcattc tactgatttt tacacaaaat
      961 taacatttga aacgggaaac aaaacaaacc caatgccaat tcaaaacgaa gtgcataggg
     1021 attaattatc accatctcaa aaaattacca aatcaaacaa ataggctaca ataaaacata
     1081 aaaattaaat gtattatttt tatctcccga agacaacaaa aaactaaagc gagaaaaacg
     1141 atcaatcaat tattggctca atttgagttt aatttattaa aatatatacc tttgtccctc
     1201 taaaaaataa cagcaatgtc gaatactata agaattaaga aaggtttaga tattaagctg
     1261 caaggaaaag ccgaactggt ggttgaaaca ccattatcgt cggcaacatt tgctatcaaa
     1321 ccttccgatt ttaaggggct agtcccaaaa ctttccgtaa aggctgatga cattgtgaaa
     1381 gccggaactc caattattca cgacaaatac aaccccgaga tacaatttgt atcgccggta
     1441 agtggtaaag tagtgaatat caatcgcggc gaccatagaa tgatactcga agtgatcatt
     1501 gaatcagaca acaaaaacga ttcaattgag tttaatgttt cgggttggaa atcggccggt
     1561 agggaagaaa ttatcaaaca gctgctagac agcggtcttt ggccttacat catccagcgt
     1621 ccatttgggc tgatagccaa gccaagcgac actccgcgcg acatttttgt ctcgggattc
     1681 gattctgccc cattggcacc cgatttcgac tttatcgtcg gaactgaaac cgcagcgttt
     1741 caaaccggcc tcgaggtgct ttcgaaatta acatcgggaa aagtattcct tggtctgcgc
     1801 aacggttcaa aactttcatc aatgaaaggg gtggaaatca attattttga aggaccacat
     1861 ccagcaggca acgttggcat tcaaattcat catctgaaac cgatcgacaa agggatcaac
     1921 gtatggacac ttagtcctca ggcagtactt ttcattggcc gtttattcac caacgggaag
     1981 ctcgattttt cggaaaccat tgcaatcaca ggttccgaag taaaatcgcc atgctaccta
     2041 aaaacaattt ctggtgctga aattaattcg ctggttgccg gaagaacaaa aaaagaagtt
     2101 aacgaacgaa ttatctcggg caatgtactc accggggtaa aaagcgattc aaacggatac
     2161 cttggttttt accataatca aatcacggta atacccgaag gcgacaatta cgaatttatg
     2221 gggtgggcaa tgccacgctt caacaaattc agcgtttcgc gagcttattt ttcatggctc
     2281 aaccctggga aaaagtaccg tgccgatgcc aacctcaatg gcgaagaacg agcctatgta
     2341 atgacaggac aatacgaaaa agtattgcca atggaaatcc ttcctgtaca tcttataaag
     2401 gccattattg ctgaagactt cgacaaaatg gaacaacttg gcatttacga agttatagaa
     2461 gaagacatgg ctctctgtga atatgtttgt acttcgaaaa ttgaggttca gcagatactc
     2521 agaaaaggaa tcagcatggt ggtgaaagaa cttggttaac aagataaaat gaacaagaaa
     2581 tagatgaatt ctcttagaaa atatatcgat aagaataaac cgctttttca gaaaggtggc
     2641 aaattcgaaa agctaggatc gacgttcggc gcgttcgaaa cgttcctgtt cactcctaac
     2701 cacacagcaa aatcggggtc acacattcac gacgtgatcg acctgaaaag aaccatgatt
     2761 gttgttgtgt tagctttgtt accagccctt cttttcggct gctggaatgt tggttaccaa
     2821 aacgggctag catttggcat tgaacgctcg ctgctgggac acttcttctt cgggttttgg
     2881 aaattcctcc cgttgtttat tgtttcttat gtagtagggc ttggcattga attcgcctcg
     2941 gcacaaatac gagggcacga agtaaacgaa ggatatttag taaccgggat acttatacca
     3001 ctcatcattc ccataaatac tccgctgtgg atgttggcgc ttgccgttgc ttttgctgta
     3061 atttttggaa aagaaatatt tggcggcacc ggatacaaca ttatgaatgt ggctcttatt
     3121 gcacgtgcat ttcttttctt tgcataccct tcagaaatgt cgggcgacaa ggtttggata
     3181 tatggactac ccgaccaagc tgctatagac ggtttctcag gtgctacgcc actggctcaa
     3241 ctcgctcaag gtgcaacaca aaatatggcc tcaatgagcg atcttttcat gggctttatt
     3301 cccgggtcca ttggtgaaac ctcgaaatta gccatattaa ttggcgccgc aattcttctt
     3361 ttcacaggaa ttgccagttg gaaaattatg ctttcgactg ttattggtgt cctctccatt
     3421 ggacttttat gcaacgcatt cgcgctcaat gaatatatgg cagtgccagc atattaccat
     3481 ttacttatcg gaggttttgc ttttggtgcc gtgtttatgg ccaccgaccc ggtaacagct
     3541 ccgcacactg aaaaaggcaa atggatttac gggttcttga tc
//
LOCUS       DAOPUW010000120         3564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_26663_length_3564_cov_2.740952, whole genome shotgun sequence.
ACCESSION   DAOPUW010000120 DAOPUW010000000
VERSION     DAOPUW010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26663_length_3564_cov_2.740952"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2163)
                     /locus_tag="PKJ43_03025"
     CDS             complement(<1..2163)
                     /locus_tag="PKJ43_03025"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNZ71563.1"
                     /translation="MFSQFLFFTSMYNTCRNAFRFVLVCAIGIGVELSASGQSITITG
                     KVVDKQTEETLPFANVYIKGKPTTGITTDYDGNYTLTIDQPEDSIGASSIGYITVFKP
                     LQKNNSKQMIVLPLDRSDYSLMEVVIKPGENPANILMRKVIANKGKYNKNKLNSYGYE
                     VYNKMEIDLEEINDKFKNRKVFKPFKFVFNNMDSVSEEKPFLPFFMTETMSDFHHRKN
                     PLDNREIIKASKVSGVNNASVTQFLGSMYVQFDIFEDWILLLNQSFVSPISNVGLSSY
                     RYYIVDSAFIGNKWCKKIQFMPRRKGELTFEGDMWIADSAFAVKQLSMKISKDADVNF
                     VKKISIYNEFESLSDSIWLLKKDKLVINFIHAKNAPGLIGRKTTSYSKFTLEEDQKTI
                     DSLFKKQKADITIKDNANEYSDKDWQQFRHDSLSKNENRIYGMIDTLKTLPVVKSWLT
                     AFQTIYLGYYDIGPLSIGNFWTFLDVNPVEGWRFKYGMGTSNKFSKHIMLNAYVAYGI
                     KDRKVKYGGEFLWIIRKKSRESLSGSYKNDVTYTTDFDRFYSSSNILNSIPGIRRVEH
                     NGVIPYKIVGVRELKFNYFKETNFGYSITAGFANRFYKPLGKFEFNYATNGNDLRKNQ
                     TIQSFTTTEFSFTNRFAWQEKFISGEFNRTSLGSKYPVLVLRYTLGLKRWAKGDFNYH
                     KLSFAMDDVQPCGPLGRLYWRIEVGKTFGRVPFLLLYTP"
     gene            2230..3003
                     /locus_tag="PKJ43_03030"
     CDS             2230..3003
                     /locus_tag="PKJ43_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNZ71564.1"
                     /translation="MSELNVLTYNVNGVRAAIKNGLLQWLSDKDFDVICLQEIKVALE
                     DFDPKPFEALGYTCYLFPAQKKGYSGVAIFSKIKPDNVVRGMGVSEYDAEGRNIRIDI
                     GDLSILSAYFPSGTTGDERQSVKMSYLDAMYDYLHELKKTRPNYLVCGDFNICHTEID
                     IHNPLQNKDTSGFTPEERAWMTKFFDSGFVDTFRVLNPQPNQYSWWSYRANARQNNKG
                     WRIDYIAASKTLEGKVKSSRMYPDAVHSDHCPVFCKVEL"
     gene            complement(3069..3260)
                     /locus_tag="PKJ43_03035"
     CDS             complement(3069..3260)
                     /locus_tag="PKJ43_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNZ71565.1"
                     /translation="MENGTVKFFNDEKGFGFIKDANSSKEYFVHSSGLTTRIKEGDNV
                     MFDLQDGKKGLNAVNVKLA"
BASE COUNT         1033 a          703 c          711 g         1117 t
ORIGIN      
        1 aggagtgtaa agtaaaagaa atggaacccg accaaaagtt ttccctactt cgattcgcca
       61 atacagtctt ccgagcggac cgcaaggttg cacatcgtcc atagcgaaag agagtttgtg
      121 atagttgaaa tcgccttttg cccatcgctt caagcctaat gtgtagcgca acaccaacac
      181 agggtacttg ctgcctaatg aagttcggtt gaactcgcca ctgatgaact tttcttgcca
      241 agcaaaacgg ttggtgaaac taaactcggt ggtggtgaac gattgaatgg tttggttttt
      301 tcttaaatcg ttaccattgg tagcatagtt gaattcgaac ttacctaaag gtttgtagaa
      361 tcgattcgca aatcccgctg taatggagta cccaaagttg gtttctttga aataattgaa
      421 tttcaattcc cgaacaccca caatcttgta tgggattacg ccattgtgtt ctactcttcg
      481 aatgccgggt atggaattca aaatatttga tgaggaataa aagcgatcaa aatcagtagt
      541 gtaggttacg tcatttttgt agctgccgga aagcgattcc cgtgattttt tacgaataat
      601 ccagagaaac tcccctccgt attttacctt tcgatctttg atgccgtaag ctacgtatgc
      661 gttgagcata atgtgtttac tgaatttatt gctggtgccc ataccgtatt taaaccgcca
      721 gccttccaca ggatttacat caaggaaggt ccaaaaatta ccaatactta gcgggccgat
      781 atcatagtat cctaagtaga tggtttgaaa agcggtcagc caacttttca ccactggcag
      841 ggtttttagt gtgtcgatca tgccataaat ccggttctcg tttttcgata atgaatcgtg
      901 acggaactgt tgccaatcct tatcggaata ctcattggca ttgtctttaa tggtgatatc
      961 agctttctgt tttttaaaca aactgtcaat cgttttttga tcttcctcca gtgtgaactt
     1021 actatacgaa gtagttttcc gtccaattaa tccgggtgca tttttggcat gaatgaaatt
     1081 gataactaat ttgtcttttt tcagtaacca tatagaatcg ctcaaacttt caaactcgtt
     1141 gtagatgctg attttcttta caaaattcac atccgcatcc ttcgaaattt tcatgcttaa
     1201 ctgtttcacg gcaaaggccg aatcggcaat ccacatgtcg ccttcaaacg taagctcccc
     1261 ttttctgcgc ggcatgaatt ggattttttt gcaccactta ttgccgataa aagcgctatc
     1321 cacaatgtag taacgatacg atgataaccc tacattagaa atgggcgaaa caaaactttg
     1381 attgagcaat aaaatccaat cttcaaaaat gtcgaactga acatacatag agcccaaaaa
     1441 ctgggttaca ctggcattgt ttacgcctga aactttagaa gctttgatga tttcgcggtt
     1501 gtcgagcgga ttctttctat ggtgaaaatc cgacatggtt tctgtcatga aaaacggcaa
     1561 aaaaggtttc tcttctgata cactatccat gttgttaaac acgaatttga agggtttgaa
     1621 aacttttctg tttttgaatt tgtcatttat ctcttcaagg tcaatttcca ttttgttata
     1681 tacctcgtag ccgtatgagt tcagtttatt tttgttgtac ttgcccttgt tggcaattac
     1741 ttttcgcatt aaaatatttg cagggttttc gcccggttta atcaccactt ccatcaacga
     1801 atagtcggaa cgatcaagcg gcaaaacaat catctgcttg ctgttgtttt tttgcaaagg
     1861 tttgaacacc gtgatataac caatcgatga agcgccaatc gaatcttcgg gctgatcgat
     1921 agtgagggtg taattgccat cgtagtcggt agtgatgccc gtggttggct tacctttaat
     1981 gtaaacgttg gcaaaaggca acgtctcttc ggtttgttta tccaccactt ttccagtgat
     2041 ggtaatggat tgcccggaag cagaaagttc aaccccaatt cctattgcac aaaccaatac
     2101 aaaacgaaat gcatttcggc acgtgttgta catggaggtg aagaataaaa attggctgaa
     2161 cacttctttt ttttagtttc gtgaagcgtt caaaataatt cttttcttat aacttttaag
     2221 tgttttttaa tgagtgaact aaatgtccta acatacaacg tgaatggtgt tcgagcagcc
     2281 ataaaaaatg gtttattgca atggctgtcc gacaaagatt ttgatgtgat ttgtttacaa
     2341 gaaatcaaag tagcattgga agatttcgac ccaaaacctt ttgaagcatt gggttatact
     2401 tgttatttgt ttcctgcaca gaaaaaaggc tacagcggtg tagctatctt ttctaaaatc
     2461 aaacccgata atgtagtgcg cggcatgggt gtatcggaat atgatgctga aggaagaaat
     2521 attcgaattg atattggcga cttatctatt ctttcggctt atttccctag cggcacaacc
     2581 ggtgacgaac gtcaaagtgt gaagatgagt tatttggatg cgatgtacga ctacctccac
     2641 gagttgaaaa aaacacgtcc gaactattta gtatgtggcg attttaatat ttgtcatact
     2701 gaaatcgata ttcataatcc gttgcagaat aaagacacat ccggtttcac acccgaagag
     2761 cgcgcgtgga tgacgaagtt ttttgattcg ggatttgtag atacgtttcg ggtcttgaat
     2821 cctcaaccca atcaatattc gtggtggagt tacagagcca atgctcgtca gaataacaaa
     2881 ggctggcgta ttgattatat agcggcaagc aagactttgg aagggaaagt aaaatcatct
     2941 cgcatgtatc ctgatgcagt gcactcggat cattgtcctg tgttttgcaa ggtggagctt
     3001 tagcagttgt acaaaaaaaa gaaacccgcc aatggcgggt ttcgatataa aattgaatgc
     3061 gcgtaaaatt aagctagctt aacgtttact gcgtttaatc cttttttacc atcctgaaga
     3121 tcgaacatta cgttatcgcc ttctttgatt cgggttgtta aaccagagct atgcacgaaa
     3181 tactctttag aagagtttgc atctttgata aatccaaatc ctttctcgtc attaaaaaat
     3241 ttcactgtac cgttttccat aaaaattgtt ttttgcgttg ttactaatta aacgcatgct
     3301 caccaacaac aaacgtgacc ataccgtgca aagatagcaa tattttcatc attggccggt
     3361 ttatattcct tttgcaacaa ggctttcttc cattccacaa aaaaaatgcg tagaatagca
     3421 ctagtgaaaa acaaaacaaa caacgcgcaa tacagtgtaa tatcgatgct cttttttgga
     3481 gcggtaatgc tgctttttta ttcctgcaac aacagccagg agaccatgaa attcaatcat
     3541 cctgtcccaa aaggtgatgt aaag
//
LOCUS       DAOPUW010000121         3559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_26711_length_3559_cov_3.686358, whole genome shotgun sequence.
ACCESSION   DAOPUW010000121 DAOPUW010000000
VERSION     DAOPUW010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26711_length_3559_cov_3.686358"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PKJ43_03040"
     CDS             complement(<1..667)
                     /locus_tag="PKJ43_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ71566.1"
                     /translation="MSSETFHHALYVDNEKCIRCTHCVKVCPTEAIRIVNAQVVIRED
                     RCVDCGECMRACPHKAINIEHDDLGQIWKYKYRVLLFPSVFLGQFPEKITEDQIYASL
                     LEIGFTHAYEVEQPIEVLKNKLREQIKNNKTEYPVISSFCPAIIRLIRIKYPSLTENI
                     SRVKAPHDLAAHWVLQKVFNRGIDPAEVGIFYITPCAAKITAVKSPLGEKESIITGII
                     NPKQ"
     gene            complement(657..1082)
                     /locus_tag="PKJ43_03045"
     CDS             complement(657..1082)
                     /locus_tag="PKJ43_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ71567.1"
                     /translation="MVKLKYDIEGGNFNRAGNASSQVKRVLKQLNIDQRLIKKIVVAL
                     YEAEVNIVAHAYEGTMEINISPEKIDMILQDKGPGISNVEQAMQEGFSTASQKVREMG
                     FGAGMGLPNIKKNTDLMEIETSVGVGTTLRMTKYLNNVE"
     gene            complement(1082..1423)
                     /locus_tag="PKJ43_03050"
     CDS             complement(1082..1423)
                     /locus_tag="PKJ43_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HNZ71568.1"
                     /translation="MTLTSIIELLEAKVVTGNMCHEEVETGFCSDLMSDVLTLDTDKM
                     LLITGTANIQTIRTAEMADVKIILLVRKKEASPEMIKLAEETNITILQTAFSSFRTCG
                     ILYSFGLKPVY"
     gene            1655..2545
                     /locus_tag="PKJ43_03055"
     CDS             1655..2545
                     /locus_tag="PKJ43_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNZ71569.1"
                     /translation="MLSIDFKIVDQLPFPVFYRTTEGIVEFSNQAFCRIIGLSSNLIA
                     GRQLSDIMPHSQADFFSIKDKELLSSEVDQHYEVSLKYSDGEEHYVLVTKTLLRDEHG
                     ETIGIVGSIADISERKNLQNLLAESENEKLISSTMLQKIRAGIVIVNSNLKIIDSNLG
                     FAKLVGGENEELFETIPGLKGADLEMLVPGVIVDMFRSVFESGESRIERDITYQNKLL
                     SVGIVTIHKNQVAGALIRDLSAPMLERAEIIERAKQVNRKNMKTVQQIAYLLGENAAQ
                     TEELLNSIIESQKYNVEETQ"
     gene            2549..>3559
                     /locus_tag="PKJ43_03060"
     CDS             2549..>3559
                     /locus_tag="PKJ43_03060"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNZ71570.1"
                     /translation="MNQNYHTEIEFQQLTPKGQEVCGDIFLSRRVKEEGRTVLVLSDG
                     VGHGIKASVLATLTATMALNYSSFHTKPEIAAQIIMDALPSSGETPNYATFTMIEIES
                     NGLIRTINYDNPPVIILRGNQRFIPQKIYETPVRGEENQGKMLRCMEFYALKEDRIIF
                     LSDGVTQSGIGSPDLKMGWGHQQVVDFILKQIKNQPKLSATKLAQKIVNRALANDKYS
                     LRDDTSCGVVYFREPREVMLITGPPFYKIKDQGFIQRIKEFTGMKMICGGTTAEIIAR
                     ELGLKLDMVQYFADMSLPPASILEGFDLVTEGILTISKVEEILENYSAETRLYDSPAE
                     EVA"
BASE COUNT         1027 a          664 c          754 g         1114 t
ORIGIN      
        1 tttgtttggg gtttatgatt ccggtaataa tcgactcttt ttcaccaaga gggcttttta
       61 ctgctgtaat ttttgcagca cagggtgtaa tgtaaaaaat tcctacctca gcggggtcaa
      121 taccacggtt aaatactttc tgcagaaccc aatgagcggc caggtcgtgt ggcgctttga
      181 ctcgtgaaat gttttcggta agcgatgggt atttaattcg gataagccgg ataatagctg
      241 ggcagaacga cgaaataaca gggtattccg ttttattgtt tttgatttgc tcacgaagtt
      301 tatttttcaa tacctcaatg ggttgttcta cttcgtaagc gtgggtaaac ccaatctcga
      361 gaagtgaagc atagatttgg tcttcggtta ttttttcggg gaattggcca aggaatactg
      421 aagggaagag aagtaccctg tatttatact tccatatttg ccccaagtca tcgtgttcta
      481 tgttaatggc tttgtgcggg catgccctca tgcattcacc gcaatcgaca caacggtctt
      541 cgcggattac gacctgtgca ttcactatgc gtatggcttc tgttgggcaa accttaacgc
      601 aatgtgtaca acgtatacat ttttcgttgt cgacatacag ggcatgatga aatgtttcac
      661 tcgacattgt tcagatattt ggtcatgcgg agcgttgtac caacgcctac cgatgtttct
      721 atttccatta aatcagtatt ctttttaatg tttggcaacc ccataccagc accgaacccc
      781 atttcacgta ctttttgcga tgcagttgaa aatccttcct gcatggcttg ttctacgttt
      841 gaaatgccag gacctttgtc ttgcagtatc atgtctattt tttcgggaga aatgtttatt
      901 tccattgtcc cttcatacgc gtgggcaaca atgtttactt ctgcttcata taatgcaaca
      961 actatttttt taatcaaccg ctggtctata tttagttgct tgagcactct ctttacctgg
     1021 ctcgatgcat ttccggcacg gttgaaattg ccaccttcta tgtcatactt caatttgacc
     1081 atcaatatac aggttttaat ccgaaactgt acaatattcc acaagttcta aatgaagaga
     1141 atgcggtttg cagaatcgta atatttgttt cttcggccaa tttaatcatt tcgggtgagg
     1201 cttccttttt tcgcaccaac aatataattt ttacatctgc catctcggcg gtcctgattg
     1261 tttgaatatt cgctgtgcca gtgattaata gcattttatc tgtgtcgagt gtgagaacat
     1321 cgctcatcag atcggaacaa aagccagttt caacttcttc atggcacata ttgccggtta
     1381 caactttcgc ttccaataac tcaattatcg atgtcaacgt catgcacttg aatttttttg
     1441 aattagttcc cgaattttga ggcaaaaata atcttgattc ttacaagtac aaatatgcaa
     1501 aatactctcg actcaaatta gtcgaaaatt atactatcgg gactggaatt ttatttctat
     1561 ttgttgataa taatcttgtg attgattatc ttaccggttg tcagtaaata cgttgtgtat
     1621 gaccgtgaat aataattcat ctgaaattaa gaacatgtta tcaattgatt ttaagatcgt
     1681 cgaccaattg ccatttcctg tgttttatcg cacaactgag ggaatcgtag aattttctaa
     1741 tcaagctttt tgtcgaataa ttggactgtc atctaatttg attgcgggaa ggcaactttc
     1801 tgatataatg cctcatagcc aagctgattt tttttctata aaggacaagg aattattgtc
     1861 ttcagaagtc gatcagcatt acgaagtttc gttaaagtat agcgatggtg aagaacatta
     1921 tgttttggtt accaaaacat tgcttcgcga tgaacatggc gaaaccattg ggattgttgg
     1981 gtcgattgcc gatatttctg aacgaaaaaa tctgcagaac ctgctcgcag agtcagaaaa
     2041 tgaaaaatta atttcatcaa caatgctcca gaaaatcagg gcaggtattg ttattgtaaa
     2101 tagcaatttg aagattatcg acagtaattt gggctttgcc aagcttgtcg gtggtgaaaa
     2161 tgaagaactt tttgaaacaa ttcccgggct aaaaggtgct gatcttgaaa tgcttgtgcc
     2221 gggtgttatt gttgacatgt ttcgctctgt atttgaatca ggggaaagcc gcattgagcg
     2281 agacataact tatcagaaca agttgctttc agtggggatt gtgactattc ataaaaatca
     2341 ggtagccggt gcgctaattc gcgatttatc ggcaccaatg cttgaaaggg ctgaaattat
     2401 agaaagagca aaacaagtga accgtaaaaa catgaagact gttcagcaaa tagcctattt
     2461 actaggcgag aatgctgctc aaactgaaga gttgctgaac tcgattatcg aatcgcagaa
     2521 gtataatgtt gaagaaactc agtaaacgat gaatcaaaac tatcatacag aaatagagtt
     2581 tcaacaactt accccaaaag gacaggaggt ttgcggcgat atttttttat cgcgaagggt
     2641 aaaagaagag gggcgcacgg tattagtttt gtccgacggt gtaggacacg ggattaaagc
     2701 cagtgttttg gcaacactta cagctactat ggcattgaat tattcaagtt tccatacaaa
     2761 acccgaaatt gccgctcaaa ttatcatgga cgctttgcca tcttcaggcg aaacacccaa
     2821 ttacgctacg ttcactatga tagaaattga aagcaatgga ctcattagaa cgataaatta
     2881 tgataacccg ccagttatta ttttgcgcgg aaatcagcgt ttcattcctc agaaaattta
     2941 tgaaactcca gttcgtggag aagaaaacca agggaaaatg ttgcgttgta tggaatttta
     3001 tgcgctaaaa gaggatcgga ttatttttct ttccgacgga gtaacccagt cggggattgg
     3061 tagccctgat ctaaaaatgg ggtgggggca tcaacaagtt gtagatttta ttttaaagca
     3121 aattaagaat cagcccaaat tgtcggccac caagttggca cagaaaattg taaaccgggc
     3181 tcttgctaac gataaatatt cgctacgcga tgataccagt tgcggtgtag tgtattttag
     3241 agaacctcgg gaagttatgc ttattacggg tcctccattt tataagatta aagaccaggg
     3301 ttttattcag cgaataaagg aattcaccgg catgaaaatg atttgcggag gaactactgc
     3361 agagatcata gcccgtgaac ttgggcttaa actggatatg gttcagtact ttgcagacat
     3421 gtcattgcca cctgcttcga ttctcgaagg attcgatttg gttactgaag gcattcttac
     3481 gatatcgaag gtggaagaaa tattagaaaa ttattctgct gaaactaggt tatatgattc
     3541 acctgccgag gaagtggca
//
LOCUS       DAOPUW010000122         3547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_26843_length_3547_cov_3.086483, whole genome shotgun sequence.
ACCESSION   DAOPUW010000122 DAOPUW010000000
VERSION     DAOPUW010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26843_length_3547_cov_3.086483"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PKJ43_03065"
     CDS             <1..382
                     /locus_tag="PKJ43_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNZ71571.1"
                     /translation="DRTKHHILPLSKFCLMQITRQRVRPEEHVDTSEVCPLCRGTGKI
                     TPTILFIDEIENKIDYICSDLNHKRVVIKVHPFVEAFLKKGLISILRRWRWKYLKKIE
                     VASDENISLLEAHFFDGNNEEIIF"
     gene            579..2576
                     /locus_tag="PKJ43_03070"
     CDS             579..2576
                     /locus_tag="PKJ43_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNZ71572.1"
                     /translation="MMIKRILLIAALILLGQIGMAQVVNPVKWSFSKKQLSDTQAELQ
                     FKAVIEKGWHLYSDKLPEGGPIATSVSYDDTTLFKRIGSLAPSKPPIKVFDKTFNMEL
                     EYFGDEVTFIQKIEFEAPVSISGHIEYMSCNDETCTPPTEADFSFQLVAKKDNKSEVL
                     PVIENGKPASGTENRGLMLFFFFSFLAGLAAILTPCVFPMIPMTVSFFMHSNKSKAKA
                     RFNAIVYGISIIAIYTIIGTLVAVLFGPDAANWLSTHWLPNIIFFVIFIVFAFSFFGM
                     FEIVLPSWMVNKADKGADRGGISGSFFMALTLVLVSFSCTGPIVGAILVESAGGQVLK
                     PIIGMFGFALAFALPFTLFAIFPQWLSNLPKSGGWLNSVKVVLGFIELALGLKFLSVA
                     DQTYHWGLLDREVYIAIWIVIFTLMGIYLMGKIKFAHDSDVKHVSVPRLMMSIVTFSF
                     VVYLIPGMFGAPLKAISGYLPPMSTHDFDLHKIIRDEVSLVTQSNATASFVNSNDEDC
                     EEPKYADLLHLPHGLKGYFDYEQGLACARAQGKPLFIDFTGHGCVNCREMEAAVWSDP
                     RVLQRLKNDFVIVALYVDDKTTLPESDWITSKYDGKVKKTIGKKYADFQISRFGVNAQ
                     PYYVVLNDAEEILVKPKARDLNPDNFVRFLDEALAAYKKGK"
     gene            complement(2595..3203)
                     /locus_tag="PKJ43_03075"
     CDS             complement(2595..3203)
                     /locus_tag="PKJ43_03075"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNZ71573.1"
                     /translation="MKTKLKTDEKIILEVQLHWFTLVVPFLIGTALVVGGIFLIPFGL
                     ILSAAGALLILYKLIERKNNLWTVTNFRIVDEKGVLSNNSKESPLDKINNVAYHQSFW
                     GKIFNYGNVEIQTAAEIGATTYSMLENPARLKDTITQMQEEYKQLQARKQAQEMANAM
                     KAASQPPTVDIKEELEKIFELKMKGILTEEEYNAQKTKILSR"
BASE COUNT          891 a          724 c          763 g         1169 t
ORIGIN      
        1 cgaccgtacg aagcatcaca tccttccgtt aagtaagttt tgtttgatgc agattacaag
       61 acagcgcgtt cgccccgaag aacatgtcga cacctccgag gtgtgtccac tttgccgggg
      121 tacaggaaag attactccaa cgatcctatt tatcgacgaa atcgaaaaca agatcgatta
      181 catttgcagc gatttgaacc acaaaagagt ggtcatcaag gttcatcctt tcgttgaagc
      241 cttcctcaaa aaaggcttaa tttcgattct gcgcagatgg cgttggaaat atctgaaaaa
      301 aattgaagtt gccagcgacg aaaatatttc tttgctcgaa gcacactttt tcgacggcaa
      361 caacgaagaa attatctttt aaagaatgtt aaaccccgct tcggcggggt ttttttgtga
      421 ctatagtcac tgcttctaaa actctcactc ccttattttg ttatctttaa tccgaattag
      481 ttcggcgatt atttttttat tccattcagt caattatttg cctgtttggc tttttgcctt
      541 attaatggta tgttttattc tttaatacga attgtaacat gatgataaaa agaattcttc
      601 tgattgctgc actcatactg cttggtcaaa ttggtatggc tcaagtggtc aatcctgtaa
      661 aatggagttt cagtaaaaaa caactctcgg acacccaagc cgagcttcaa tttaaagctg
      721 ttatcgaaaa aggctggcac ctttattccg ataaactccc cgaaggtggc ccaattgcta
      781 cgtcggttag ctatgacgac actacactat ttaagcgtat tggtagctta gctccttcta
      841 aaccgcccat taaggtattc gataaaacct ttaacatgga gcttgaatat tttggcgatg
      901 aggttacttt tattcagaaa attgagtttg aagcaccggt ctcaatttcc gggcatattg
      961 agtatatgag ctgtaacgac gagacttgta ctcctcctac cgaagccgat ttctcttttc
     1021 aacttgtagc aaaaaaagac aacaaaagtg aagttctgcc tgtaatcgag aacggtaaac
     1081 ctgcatcggg caccgagaac cgtgggctga tgcttttttt ctttttttcg ttcctcgccg
     1141 gcttggcagc cattttaacc ccatgcgtgt tcccaatgat tccgatgacg gtttcgtttt
     1201 tcatgcacag caacaaatca aaagccaagg ctcgatttaa tgccattgtt tatggtattt
     1261 ctatcattgc aatctatacc ataattggca cactggtagc cgttcttttt ggccccgatg
     1321 cagcgaactg gctcagcacc cattggttgc ccaatataat cttttttgtc atttttattg
     1381 tgtttgcctt ttctttcttt ggaatgtttg aaattgtact tccaagctgg atggtaaaca
     1441 aagccgataa aggagccgat cgcggaggta tttcggggtc gttcttcatg gcgttaacat
     1501 tggtactggt atcgttctcg tgtaccggtc ctattgttgg tgccattttg gtggagtcgg
     1561 cagggggaca ggtacttaag ccaattattg ggatgtttgg ttttgccttg gcttttgcgt
     1621 tgccgtttac tttgtttgcc attttcccgc aatggctaag taatctcccg aagtctggcg
     1681 gctggcttaa ttcggtgaaa gtggtgcttg gctttatcga actggcgtta gggctcaagt
     1741 tccttagcgt ggccgaccaa acctatcatt ggggactact cgaccgtgaa gtttatattg
     1801 ctatttggat cgtaattttc acgttgatgg gaatctatct tatgggtaaa atcaagtttg
     1861 cccacgacag cgatgtgaaa cacgtttcgg tgccacggtt aatgatgagc atcgtaactt
     1921 tctcgtttgt ggtttacctt attccgggca tgtttggtgc tccgttgaaa gctatttcag
     1981 gctatcttcc cccaatgtcg acccacgatt tcgacttaca taagatcatt cgagatgaag
     2041 tttcgctggt aacacaaagc aatgcaactg cctcgtttgt caattccaac gacgaagatt
     2101 gtgaagaacc caagtatgcc gatttgttgc atttgcccca tggcttgaaa ggatatttcg
     2161 attatgagca aggattggct tgtgcccgtg cgcagggcaa accgttattt attgacttca
     2221 cggggcatgg atgcgtgaat tgccgcgaaa tggaagctgc tgtgtggagc gacccgcgtg
     2281 tgttgcagcg cttgaagaac gattttgtaa ttgtggccct ctatgttgac gataaaacta
     2341 ccttgcccga gtctgactgg attacatcga aatacgatgg aaaagtgaaa aagaccattg
     2401 gaaaaaaata tgccgatttt caaattagcc gtttcggggt taatgcccaa ccgtattatg
     2461 tggtgctcaa cgatgccgaa gaaattttgg taaagcctaa agcccgcgac ttgaaccctg
     2521 ataattttgt ccgctttctc gacgaagcgc ttgctgcata taaaaaaggg aagtaattgt
     2581 acttccctct tattttatct gcttaatatt tttgtctttt gagcattgta ttcttcctcg
     2641 gtgaggatgc ctttcatttt tagctcaaaa atcttttcaa gttcttcttt tatatcgact
     2701 gttggtggtt gactggctgc tttcattgcg ttggccatct cttgggcttg ttttcgggct
     2761 tgaagttgct tgtattcttc ctgcatttga gtgatggtgt cttttaaccg cgcagggttt
     2821 tcgagcatgc tgtaggtagt tgcaccaatc tcggctgctg tttggatttc tacattgcca
     2881 tagttgaata tttttcccca gaatgactga tggtaagcga cattgtttat tttgtcgagc
     2941 ggactttctt tactgttgtt cgatagtacc cctttttcgt ctactatgcg gaagtttgtg
     3001 actgtccaaa ggttattttt tcgttcaata agtttgtata gaattaataa agctcctgct
     3061 gccgagagga ttagtccaaa tggaatgaga aatatgcccc caacaaccaa cgcggtgcca
     3121 attaaaaagg gaacaaccag ggtaaaccaa tgtagctgaa cctcgaggat aattttttcg
     3181 tctgtcttta gtttcgtttt cattgttagt cttcatttta agttgcttat tcccgtttgt
     3241 gttttgctgt gtttgattca taaacacaac atgcacttcg aaaacatgct tgtgaaaaaa
     3301 tcaatatgag atgcgttgca tttgcaaaat cagcctcaaa ttacatattt ttttaatgga
     3361 atgagaggcc gatcatattt ttttcagcaa ttgcattaaa attttaatta gctgtaaatt
     3421 agcttagtat tcattatcta cgtcggtcag tacttcgaat ccggtttcgg taacgagaat
     3481 ggtgtgctca aattgtgctg acaattttcc atcaacagta cgggctgtcc atccatcgaa
     3541 tttatca
//
LOCUS       DAOPUW010000123         3537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_26952_length_3537_cov_2.652499, whole genome shotgun sequence.
ACCESSION   DAOPUW010000123 DAOPUW010000000
VERSION     DAOPUW010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26952_length_3537_cov_2.652499"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PKJ43_03080"
     CDS             <1..255
                     /locus_tag="PKJ43_03080"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNZ71574.1"
                     /translation="AKLTNAATPRNLKVSFFWIFYAPYNILELDENYQYALIGSSSEK
                     YLWILSRIPNMDDTTYNMLLSKAKARGYDVSKLIKVVHPK"
     gene            328..>1516
                     /gene="ftcD"
                     /locus_tag="PKJ43_03085"
     CDS             328..>1516
                     /gene="ftcD"
                     /locus_tag="PKJ43_03085"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNZ71575.1"
                     /translation="MKQCIECVPNFSEGRNMEIIGQITREIETTDGVKVIGVEPGKAA
                     NRTVITFTGTPEAVCEAAFRMVKIASELIDMRKHQGEHPRFGAIDVLPLIPLYGITME
                     ETVELTRELGKRIGEELGIPIYCYEFAAFEEKRRNLASCRSGEYENLPQKLTDPQWKP
                     DFGPASFTPRVAQTGAIALGARNILLAYNVNLNTTSAQIASKIAAEVREKGTVKRTGN
                     LPDGEIIKDEHGKTMYEPGLLKGVKGIGWYIEDYGIAQCSYNITDLEKTSIEQVFEAT
                     CERAEKQGIKVTGSELIGLIPLQAILNAGTYFLKKQHRSTDISMEEIIQVAIDSLGLA
                     ELAPFEPKKKIIEFLIDEEKKSQLTDLSVKEYVEQTASPTPLPAGGSASATIAALGTA
                     LGTM"
     assembly_gap    1517..1526
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1527..2060
                     /locus_tag="PKJ43_03090"
     CDS             <1527..2060
                     /locus_tag="PKJ43_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HNZ71576.1"
                     /translation="TMVANILHKKNGNESLETKFSALAKEGKELINKLLKLCNKDTEA
                     YRKLMNALELPQKTTHEKQLRLENLATAAKSATEIPLELMKTGVSALKMLILMAEDGP
                     VSTLADTATGVLALCAAVRSSAITIRANAPLFHDKELISSIINEAICTENEAIELEKQ
                     VLAIVNRRLDEKGIITL"
     gene            complement(2050..2580)
                     /locus_tag="PKJ43_03095"
     CDS             complement(2050..2580)
                     /locus_tag="PKJ43_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ71577.1"
                     /translation="MPEIRKIFPSDEFLWEYTRNLLEISFPVNERRDFSRQRLLASSS
                     EIFSQGVIIENELPVGVICWWDFPNFLYIEHFVIDPMQRNKGLGEKVLKMMHEPTGRS
                     LVLEVELPVNEINNRRINFYKRNGFSLFDQDYLQPPYRSGENFLPMKVMSTNILHPVF
                     DEIKQTLYREVYGFTE"
     gene            2691..>3537
                     /locus_tag="PKJ43_03100"
     CDS             2691..>3537
                     /locus_tag="PKJ43_03100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNZ71578.1"
                     /translation="MHKLKDILLKYPGFLFVAALLVFSFFYDYQSIISKPPQSIHAWR
                     QSDCASLTLNYYQNGMRFFEPETHNLTSDGGTTGKCSTSEIPVLYYCVALLYSVFGHH
                     DIIFRLLNTLIFFVGLFYLFKLFRFVLKDIFWSIALTLLIFTAPVLVYYGNNYLSNSS
                     ALAFVFVGWYYFVRFLGEKKNRWFLLSVLAFFIGGACKVTALFSAFAICGIIVLELFK
                     KVNFGQQQKLFGKTYFYLLPFLAAFFVIGSWILYARWYNISHRCRYFSTTIFPIWDLD
                     KTGIKT"
BASE COUNT         1112 a          785 c          718 g          912 t
ORIGIN      
        1 gccaaactga ccaatgccgc cacaccccgg aacctgaaag tatcgttttt ctggatattt
       61 tatgcgccat ataacatcct tgaactggat gaaaattacc aatatgcact tattggcagc
      121 tccagcgaga aatatctgtg gatactctcg agaataccaa acatggatga cacaacctac
      181 aacatgcttc taagcaaagc aaaagcccgg ggatatgatg tctctaaact cataaaagtg
      241 gtgcatccga agtaaaatac ttatcgaggc aaagagattt ttcaaacatt caaataaaaa
      301 gctgaatacc agccataatc attggcaatg aaacaatgta tcgaatgcgt gcccaatttt
      361 agcgaaggcc gtaatatgga gattataggc cagataaccc gcgaaattga aactacagat
      421 ggagtgaaag tcattggagt agaaccggga aaagcggcca accgaacagt cataaccttt
      481 accggaaccc ccgaagcggt ctgtgaagct gcattcagga tggtgaaaat agcctcagaa
      541 cttatcgaca tgaggaaaca ccagggggaa catccacgtt ttggcgctat cgatgtgtta
      601 ccgctcattc ccctgtatgg gattaccatg gaagaaacag tggaactaac ccgagaactg
      661 ggcaaacgca ttggcgaaga acttggaata cccatttact gttacgaatt tgccgcattt
      721 gaagaaaaac gccggaacct tgccagttgt cgttccgggg aatatgaaaa ccttccgcaa
      781 aaattgactg atccccagtg gaaacccgat tttgggccag ccagcttcac cccaagggta
      841 gcacaaacag gagctattgc cctgggagcc cggaatatac ttttggcata caatgttaac
      901 ctgaacacca cgtcggcgca aattgccagt aaaattgccg cagaagtgag ggaaaaaggt
      961 actgtaaaac gtacagggaa tttgccggat ggtgaaataa taaaagatga acacgggaaa
     1021 acaatgtatg agccaggatt gctcaaaggg gtcaaaggaa ttggctggta cattgaagat
     1081 tatggaattg cacaatgctc gtacaacatc acagacctgg aaaaaacatc gattgaacag
     1141 gtttttgaag ccacatgcga aagagctgaa aaacaaggaa taaaggtaac aggctctgaa
     1201 ctcatcggac taatcccact tcaagccata ctcaatgccg gaacctattt tttgaagaag
     1261 caacaccgct caaccgacat ttcaatggaa gagattatac aagttgccat tgattctctt
     1321 ggccttgcag aattagcccc attcgaacca aaaaagaaga ttattgagtt ccttattgat
     1381 gaagaaaaaa aatcacaatt gaccgacctt tcggtaaaag aatatgtaga acaaacggca
     1441 tcacctaccc cactgccagc cggaggttca gcctcggcaa ccattgctgc ccttggaaca
     1501 gctcttggaa ctatggnnnn nnnnnnacta tggtcgccaa tattttacac aaaaaaaatg
     1561 ggaacgaaag ccttgaaaca aaattttcag cattggctaa agaaggcaaa gaactaataa
     1621 acaaattgtt gaaactttgc aataaagata ctgaagctta tcggaaattg atgaatgccc
     1681 tggaattacc ccaaaaaaca actcatgaaa aacaactcag acttgagaat ttagcaactg
     1741 cggcaaaaag cgctaccgaa attcccctgg aacttatgaa aaccggggta tcggcattga
     1801 aaatgttaat tctcatggct gaagatggcc ctgtaagcac attggccgat acagccacag
     1861 gagttcttgc cctatgcgct gcggtaagga gttctgcaat aaccatcagg gcaaacgctc
     1921 cgctttttca cgacaaggaa ctaatttcaa gcataatcaa cgaggctatt tgcactgaaa
     1981 acgaagccat tgaattagag aaacaagtac ttgctattgt aaatcggcgg ctggatgaaa
     2041 agggcattat tactctgtaa acccgtatac ttcgcgataa agggtctgct taatctcatc
     2101 aaaaacagga tgtaaaatat ttgtagacat cactttcatt ggcaagaagt tttcaccact
     2161 ccggtaaggc ggctgcaaat aatcctgatc gaacagcgaa aaaccattcc gtttatagaa
     2221 gtttattctc cggttattta tctcgttaac aggcaattca acttcaagca ccagggagcg
     2281 gccggtaggt tcatgcatca ttttgagcac cttctcgcct aaacccttgt tccgctgcat
     2341 tgggtcaatg acaaaatgct cgatataaag aaaattagga aaatcccacc agcaaattac
     2401 gccaaccggc aattcattct caatgatcac cccttgtgaa aatatctcgg atgaactggc
     2461 caacaaacgc tgtcgggaaa aatcacgccg ctcattcacc gggaatgata tttcgagtaa
     2521 attccgggtg tactcccaca aaaattcatc ggaaggaaat attttacgga tttctggcat
     2581 tgcatcgatt tatatctcaa caaaaatact ttctctgatc attgtttcat aaacttgctg
     2641 aaatataaaa tgaacatatc tttatactcg aaaataaaaa ttaaaacctt gtgcataaac
     2701 ttaaagacat actactgaaa tatcccggat tcctttttgt tgcagcgttg ctcgtgtttt
     2761 cttttttcta tgattatcaa tcaattatta gcaaaccccc acaatcgata catgcatgga
     2821 ggcaatcaga ttgcgcctcg ctgaccctca attattatca aaacgggatg cggtttttcg
     2881 agcccgaaac acacaacctc acctccgacg ggggcaccac gggcaaatgc agcaccagcg
     2941 aaatccctgt actttattac tgcgtggcct tgttatattc agtatttgga catcacgaca
     3001 ttattttcag gctcttaaat acgctcattt tctttgtcgg tcttttctat ctttttaagc
     3061 ttttccgttt tgtgctaaaa gacatattct ggagcattgc gcttacccta ttgattttca
     3121 cggctccggt actagtatat tacgggaaca actacctgtc gaactcatcg gcgctggcct
     3181 ttgtctttgt aggctggtat tattttgtcc ggtttctcgg cgagaagaag aatcgctggt
     3241 tcctgctctc ggtgctggcc tttttcatag gcggggcatg taaggttacg gcccttttca
     3301 gcgcttttgc catttgtggg atcattgtat tggaactctt caaaaaagta aacttcgggc
     3361 aacaacaaaa gctttttggg aaaacgtact tctatcttct cccattcctt gctgcctttt
     3421 tcgtaattgg cagttggatc ttatatgccc gctggtacaa catttcacac cgttgcagat
     3481 acttttcaac cacaattttt ccaatatggg atttagacaa gacaggcatt aagacag
//
LOCUS       DAOPUW010000124         3536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_26965_length_3536_cov_2.725079, whole genome shotgun sequence.
ACCESSION   DAOPUW010000124 DAOPUW010000000
VERSION     DAOPUW010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26965_length_3536_cov_2.725079"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PKJ43_03105"
     CDS             <1..200
                     /locus_tag="PKJ43_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNZ71579.1"
                     /translation="NARNIVNTWIRTSGKFDAVIDFDKIMRNPSDTITLMPDLHTGDF
                     LHPNEAGYQRMGEAIDLTLFK"
     gene            complement(389..1678)
                     /gene="eno"
                     /locus_tag="PKJ43_03110"
     CDS             complement(389..1678)
                     /gene="eno"
                     /locus_tag="PKJ43_03110"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNZ71580.1"
                     /translation="MMYIEEIVGREILDSRGNPTVEVEVILESGVKGIAAVPSGASTG
                     ENEAIELRDGDKSRYLGKGVLKAVDNVNKVIAPELEGWNVMDQVAIDKKMIELDGTKT
                     KSKLGANAILGVSLAVAKAAANYLEIPLYRYIGGVNAKSLPVPMMNIINGGSHSDAPI
                     AFQEFMIRPVGAPSFREALRYGAEVFHNLKKIFHDRGLSTAVGDEGGFAPTLNGTEDA
                     LESIIKAIENAGYKPGVDITIGLDCASSEFYKNGIYDYSKFEGANGAKRTSAQQVDFL
                     AELVAKYPIDSIEDGMAEGDWEGWVALTNKLGGKCQLVGDDLFVTNVEYLKKGIEMGA
                     ANSILIKVNQIGTLTETLDAIEMAHRAGYTSVTSHRSGETEDATIADIAVATNSGQIK
                     TGSLSRSDRMAKYNQLLRIEEELGEEAIYGYKKVYRK"
     gene            2103..2795
                     /locus_tag="PKJ43_03115"
     CDS             2103..2795
                     /locus_tag="PKJ43_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71581.1"
                     /translation="MSFTHEVEGMICVAQGANHGPAPIPEEGRWVKAKEVKDISGLTH
                     GVGWCAPQQGACKLTLNVKEGIIQEALVETIGCTGMTHSAAMAAEILPGKTILEALNT
                     DLVCDAINTAMRELFLQIVYGRTQTAFSEGGLPIGAGLEDLGKGLRGVTGTLYGTVAK
                     GPRYLEMAEGYITKIGLDAGNEIIGYEFVSLGRMMDMIKTGMDAGEALKKASGKYGRM
                     EDAVKVIDPRKE"
     gene            2808..>3536
                     /locus_tag="PKJ43_03120"
     CDS             2808..>3536
                     /locus_tag="PKJ43_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HNZ71582.1"
                     /translation="MPLFESYDRRIKQINKFLNDNGISSIEEAKKICDDKGVDVYNLV
                     KGIQPIAFENAMWSYIVGAAVAIKRGQTNAADIATTLGEGLQSFCIPGSVADDRKVGL
                     GHGNLAAMLLREETKCFAFLAGHESFAAAEGAIGIAKSANRVRKEPLRVILNGLGKDA
                     AKIIARINGFTYVQTDFDYFTGELKEVQRIAYSTGDRAKVNCYGANDVREGVAIFHSE
                     GVDVSITGNSTNPTRFQHPVAGTYK"
BASE COUNT         1039 a          752 c          751 g          994 t
ORIGIN      
        1 aaaatgcccg gaacatagta aacacctgga tccgcaccag tggcaagttt gatgctgtaa
       61 ttgattttga caagattatg cgaaacccca gtgacactat cactctcatg cccgacttac
      121 ataccggaga ttttcttcac cctaacgaag caggctacca acgtatggga gaagccattg
      181 acttgacgct tttcaagtaa aacaaaaata aaccgacaga tagacaacac ggttcagcat
      241 tgggccgtgt tgtttttttc tatatcagct gaaaatattg ttcttgtcca gcggcacaat
      301 ctgatttcct tttcatgcag agtttcgggt gaaaaaaaag ccattgcagg aatgcaacag
      361 ctttttaaat attatatcag taacaggatt actttctgta tacttttttg tatccgtaaa
      421 ttgcttcttc gccaagttct tcttcaatgc ggagaagttg attgtattta gccatacggt
      481 ccgaacggct caacgatccg gttttgatct gtccgctgtt agtagctacg gcaatgtcag
      541 ctatagttgc atcttcagtt tcaccagagc gatgcgaagt taccgaagtg taaccggcac
      601 ggtgagccat ttcaatagca tccaatgttt cggtaagagt accaatttgg tttaccttga
      661 taagaattga gttagcagca cccatttcaa tacctttctt cagatattcg acattggtta
      721 caaagaggtc gtcaccaacg agctggcatt ttccgcctaa tttattggtc agcgcaaccc
      781 agccttccca atcgccttcg gccataccat cttcgattga atcgatagga tatttggcca
      841 ccaattcagc gaggaaatca acttgttgag ctgatgtgcg ttttgcacca ttagctcctt
      901 caaatttcga ataatcgtaa ataccgtttt tgtagaattc agaagaagca cagtcgagac
      961 caatggtgat gtctacacct ggtttgtagc ctgcgttttc aatggcttta atgatgcttt
     1021 caagggcatc ttcagttcca ttaagtgttg gggcaaaacc accttcatcg cctactgcag
     1081 tgctcaaacc acgatcgtgg aaaattttct tcagattatg gaaaacttcg gcaccatagc
     1141 gcaatgcttc gcgaaacgaa ggagcgccaa ccggacggat catgaattcc tggaatgcga
     1201 taggagcatc ggagtgagaa cccccgttaa tgatgttcat cattggaaca ggtaacgact
     1261 tagcgttaac accaccgatg taacggtaaa gaggaatttc gaggtaattt gcagcagctt
     1321 tggctacagc cagcgaaacg cctaaaatgg cattggcacc cagcttgctc ttggtttttg
     1381 ttccgtcgag ctcgatcatt tttttgtcga tagctacctg atccattacg ttccagcctt
     1441 caagctcagg agcgattacc ttgttcacgt tatcaacagc tttgagtaca cctttcccaa
     1501 ggtagcggct tttgtcacca tcgcgcaatt ctatggcttc gttttcacca gttgaagcac
     1561 ctgacggaac agctgcaata cctttaactc cgctttcgag gattacttct acttctacag
     1621 ttgggttacc acgggaatca agaatctccc ggcctacaat ttcttcaata tacatgatta
     1681 tctttttaga gtattaaact ttaactatta atgtaaaatg aacgtgtttg ttcgaaaatt
     1741 gttttcagta cacaaaaata cgctaaaaaa aattctgtaa tgataatcac gaactaaaca
     1801 ttgatataca attctcccta cagacttttt caagaataaa ctgtaattta gtatcgaaca
     1861 attccataaa taatcagcct gctcaaagaa tatgatgaaa aaacttaatt ataaaacaaa
     1921 atctacaggc tgagagccgt taaaaaaacg ttaacaaaga cgtttaaata tcttcggcaa
     1981 aatcagcttt ttggggttta ggtacatttt taattacatt tagcgcgcct tcccggaagg
     2041 aataagggaa ggaataatgt gttgatttcc acctttaata ccaaaaatca aaaattaact
     2101 atatgagttt tacacacgaa gttgaaggca tgatctgcgt ggctcaaggc gcgaatcatg
     2161 gtccggctcc aataccagaa gaaggtcgtt gggtaaaagc caaagaggta aaagatattt
     2221 cgggtttgac ccacggtgtg ggctggtgcg ctccacaaca aggtgcatgt aaattgacct
     2281 tgaatgtaaa agaaggtatt attcaggaag cattggtcga aaccattggc tgtactggta
     2341 tgacacactc tgctgcaatg gccgctgaaa tccttcctgg aaaaacaatc ctcgaagcat
     2401 tgaacaccga cttggtttgc gacgcaatca acactgcaat gcgcgaactt ttcttacaaa
     2461 ttgtttatgg tcgtacacaa actgctttct ctgaaggtgg tctgccaatt ggcgctggtc
     2521 tggaagactt aggaaaaggc cttcgcggtg ttaccggaac attgtacgga actgtggcaa
     2581 aaggtcctcg ttacctcgaa atggccgaag gttacatcac caaaatcggt ttggatgcag
     2641 gaaacgaaat catcggttac gagtttgtta gccttggccg tatgatggat atgatcaaaa
     2701 ctggtatgga tgctggcgaa gccctcaaaa aagcttctgg caaatatggc cgtatggaag
     2761 atgcagtgaa agttattgac ccacgtaaag aataggagga atatactatg ccattatttg
     2821 aaagttacga cagaaggatc aaacagatca ataagttttt aaatgataac ggtatttctt
     2881 ctatcgaaga agccaaaaaa atatgcgacg ataaaggtgt tgacgtttac aaccttgtaa
     2941 aaggtattca gccaatcgct ttcgaaaacg caatgtggag ctacattgtt ggtgctgctg
     3001 ttgctatcaa acgcggacaa acaaacgctg ccgacatcgc tactacttta ggcgaaggct
     3061 tgcagtcgtt ctgtatccca ggttcagttg ctgatgaccg taaagtaggt ttaggtcacg
     3121 gtaatcttgc agctatgttg cttcgcgaag aaaccaaatg ttttgctttc ttagctggtc
     3181 acgaatcatt tgctgctgct gaaggcgcta ttggtattgc caaatcggca aaccgtgttc
     3241 gtaaagaacc tcttcgcgtt atcctgaatg gtttgggtaa agatgctgct aaaatcatcg
     3301 cccgtatcaa cggattcact tatgtacaga cagacttcga ttattttaca ggagaactga
     3361 aagaagtaca acgcattgct tactcaactg gcgaccgtgc aaaagtaaac tgctacggag
     3421 ctaacgatgt tcgcgaaggt gttgctatct tccacagcga aggtgttgat gtatcgatca
     3481 ctggtaactc aaccaaccca acacgttttc aacatcctgt ggctggcaca tataaa
//
LOCUS       DAOPUW010000125         3512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_27215_length_3512_cov_3.542378, whole genome shotgun sequence.
ACCESSION   DAOPUW010000125 DAOPUW010000000
VERSION     DAOPUW010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27215_length_3512_cov_3.542378"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PKJ43_03125"
     CDS             complement(<1..742)
                     /locus_tag="PKJ43_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNZ71583.1"
                     /translation="MESRENTEFQWFSNFFRHSIAVMLLIDPETHNIIDANPAAEKFY
                     GWSHSLLTQMKISHINTLSYEEIEAEMQKARSQQRSYFSFKHRKADGSICPVEVFSSK
                     ITFDGKEYLHSIIHDITEQKKAIDAQHKSNELFYAMISSTPLASFSLSPEGTVLTWNN
                     AAELMFGWSEQEAVGSILPIIPPHLFNEFHDIRQRVLNGEHIIGLEIIRQRKSGELFP
                     CRLFSSPIHDPKGKPVGLMSMIEDITQSK"
     gene            complement(763..>3512)
                     /locus_tag="PKJ43_03130"
     CDS             complement(763..>3512)
                     /locus_tag="PKJ43_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNZ71584.1"
                     /translation="GERLFATIHFLNKGASISDNEYIESFVKQAGILIERAKTSEQLA
                     QSEMKFRSVFNSTNEAIFIHEPHTGKIIDCNHAAEEMYGYTKNEFLDLEISHYSAPGE
                     EYSTKKGLEMIRQTNNHESYTFEWRNKRKDGTLFWNEISVRKAYIDNKEQVIAVCRNI
                     DERKKILEELKESEEQHRLMFETSQEGIVISQNYQLVYFNPRMLELTGYKATELQNIE
                     FLKIIDPLDHERLLRNYKSRVEGKVAEQNYEFRLLRKNGKSHWVRMSGVRLMWNGKPA
                     TFNFISDINDQKEAALHLLESEEKYRLIAENSSDVIWVFNTHTRKITYISPAIKKLRG
                     YTAEEAMKHTIKELVTNETYEEVEEQIKKQISLFNIDPINNNSFTLELLQPCKDGRVI
                     NVEASLNYSFNKFNEIEILGVSRNIDERKRILAELRDSEHKFKLLFENSPLGIYIANT
                     DGQIEDANQALLKILGSPSLKMTKGINVLQFKPLVDNGYAYHFKKSIEENRVVTIELP
                     YTTFWGKELHLSNYIVPMSNAEGKVEKVYTLMEDITQRKNDERELIKLSLVAEQSPVS
                     IVITNTKGTIEYVNPKFTEVTGYTAEEAIDQNPRMLKGGTTPKEEYKKLWDTILSGNE
                     WEGVFYNRKKNGEKYWEKATIRPIKNDSGEIISLLAMKEDITKQREAQEALVRSEARL
                     REALATKDKFFSIISHDLRSPFASIVSFSDLMADQNYTFSVDEYRQYALSLNKTAKSA
                     YTMLENLLEWSRIQRGSVKFTPERITLQAFFNRCEESTLEAAQKKSIEIVIQYSENYE
                     FTTDKNMLHTVIRNLVNNAIKFSSPGGKITITAERSQLGQMLFSVNDTGIGMDAERLE
                     KLFRIDTNVSRPGTQGEASSGLGLILCKEFVEKMGGTIWAESTEGKGSTFFFTIQQPH
                     S"
BASE COUNT          799 a          697 c          748 g         1268 t
ORIGIN      
        1 ttttcgactg ggtaatatct tcaatcatcg acatgagtcc gacaggcttg ccttttgggt
       61 cgtgtatggg tgaagaaaat aacctacatg gaaatagttc accgcttttg cgctgacgaa
      121 taatttcgag cccaataata tgctctccgt ttagtacccg ttgcctgatg tcgtgaaatt
      181 cgttgaataa atgaggtgga atgatgggca aaatggagcc aacggcttct tgttcagacc
      241 atccgaacat gagctcagcc gcgttgttcc acgtgagtac cgtcccttcg ggcgaaaggc
      301 tgaacgaggc caacggggta ctgctgatca ttgcataaaa gagttcgttg cttttgtgtt
      361 gggcatcaat agctttcttt tgctctgtaa tatcatgaat gattgagtgt aagtattctt
      421 tcccatcgaa ggtaattttg ctgctgaata cttcaaccgg gcaaattgat ccgtcggctt
      481 tgcgatgttt gaagctgaaa tacgaacgtt gttggcttcg ggctttttgc atttctgctt
      541 caatctcttc atacgagagg gtgtttatgt ggcttatttt catctgggtc aatagcgaat
      601 ggctccatcc ataaaacttc tcggcggccg ggtttgcatc gatgatattg tgagtctcag
      661 ggtcgatgag taacataacg gcaatgctat gtctgaaaaa gttgctaaac cactgaaatt
      721 cggtattctc cctgctttcc atactcgtaa attttagttt gtttatgaat ggggttgttg
      781 aatggtaaag aagaatgtgc ttcctttgcc ttcggtactt tctgcccaaa tagtgccgcc
      841 cattttttct acaaattctt tacaaaggat aagtcccaag cccgaactgg cttcgccttg
      901 tgtccctggc cgactaacat ttgtgtcgat ccggaatagt ttttctaacc gttcggcatc
      961 cattccaatt ccggtgtcgt ttacagaaaa gagcatttgc ccgagttgtg acctttctgc
     1021 tgtaattgtt atcttacccc cgggcgatga aaactttatc gcattgttta caaggttgcg
     1081 tatcacggta tgaagcatgt ttttgtcagt tgtaaattca tagttttctg aatattgaat
     1141 gacaatttct attgattttt tttgagctgc ttccagggtc gattcttcgc aacggttgaa
     1201 gaacgcttgc agggtgattc gttccggggt gaatttaaca gagccgcgct gtattcttga
     1261 ccattcgagc aagttctcga gcattgtata ggctgatttt gctgttttat tcaacgagag
     1321 tgcatattgt cggtactcat cgaccgagaa cgtatagttt tgatcggcca ttaaatcaga
     1381 aaagctaaca attgaggcaa aggggctgcg taggtcgtgc gaaatgatcg agaagaattt
     1441 atctttggtg gctagagctt ctcgaaggcg ggcttcactt ctgactaacg cttcctgtgc
     1501 ttcgcgctgc tttgtgatgt cttctttcat ggctaaaagg gatataattt ccccactgtc
     1561 attttttatt ggcctgatgg tcgctttttc ccaatatttt tctccgtttt tctttcggtt
     1621 ataaaacacg ccttcccact cgttgcccga aagtatggtg tcccaaagtt ttttatattc
     1681 ttctttgggt gttgtacctc cttttagcat tcttgggttt tgatctatcg cttcttctgc
     1741 agtgtaaccg gtaacttcag tgaatttggg gttcacgtac tcaattgttc cttttgtgtt
     1801 agtaattaca atgcttacag ggctttgttc ggctaccagc gatagtttga tgagttccct
     1861 ttcatcgttt tttcgctgtg tgatatcttc catcagggtg tacacctttt caacttttcc
     1921 ttcggcattc gacatgggaa caatgtaatt cgacaagtgc aattctttgc cccagaatgt
     1981 agtataaggc agttcgatag taaccactct gttttcttca atgctttttt tgaaatgata
     2041 ggcatagcca ttatcaacga ggggcttaaa ttgtaaaaca tttattcctt tcgtcatttt
     2101 aagcgaagga gaacctaata tctttaataa agcttggttc gcatcttcaa tttgtccgtc
     2161 ggtattggca atgtatattc ctaatggtga gttttcgaaa agtaatttga atttatgctc
     2221 actgtcgcgc aactcggcaa gtatcctttt tctttcgtcg atgttgcggc ttactcccag
     2281 tatttctatt tcgttgaatt tgttgaaact gtagttcagc gatgcctcaa cgttgataac
     2341 cctaccatct ttgcatggtt gcagtagttc taaggtaaaa ctgttgttgt ttatcggatc
     2401 gatgttgaaa agacttattt gtttttttat ttgttcttca acctcttcat atgtctcatt
     2461 ggttaccagt tcttttattg tgtgtttcat tgcttcttca gccgtataac cacgaagctt
     2521 cttaatggca gggcttatat aagtaatttt tctggtgtgt gtattgaaaa cccagataac
     2581 atctgatgaa ttttcggcaa taagacgata cttttcttca ctttcaagta ggtgtagcgc
     2641 agcttctttt tggtcgttga tatcggaaat gaagttgaat gttgctggct tgccgttcca
     2701 catgagccgt accccgctca ttcttaccca atgcgatttc ccgtttttac gcagtagcct
     2761 gaattcgtaa ttttgttctg caactttgcc ttcaacacga gacttgtaat ttcttaatag
     2821 gcgttcatga tcaagcgggt caattatttt cagaaactca atattttgta gttcagttgc
     2881 tttgtatcct gtaagttcaa gcattctcgg gttgaaataa accaattggt agttttgtga
     2941 tataacgatt ccttcttgtg atgtttcgaa catcaggcgg tgttgttctt cactttcttt
     3001 gagttcttcc aatatctttt ttcgttcatc aatgtttcgg catacagcaa taacttgctc
     3061 tttattatcg atatatgctt ttcgtacact tatttcgttc cagaataggg ttccgtcttt
     3121 acgtttgttt cgccattcga aagtgtacga ctcgtggttg ttggtttgcc tgatcatttc
     3181 aaggcctttc ttggtagaat attcttctcc gggagcactg taatggctta tttcaaggtc
     3241 aagaaattca ttctttgtgt atccgtacat ttcctcggca gcgtggttgc agtcgataat
     3301 ttttccggtg tgtggttcgt gaatgaatat ggcttcgttg gttgaattga aaaccgaacg
     3361 gaatttcatc tcactttggg ctaattgttc ggacgttttg gcgcgttcga taagtatacc
     3421 tgcttgtttt acaaacgatt ctatgtattc gttatcgctt atagacgcac ctttgtttaa
     3481 gaagtgaatt gttgcaaata gtctttctcc tt
//
LOCUS       DAOPUW010000126         3505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_27286_length_3505_cov_3.682029, whole genome shotgun sequence.
ACCESSION   DAOPUW010000126 DAOPUW010000000
VERSION     DAOPUW010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27286_length_3505_cov_3.682029"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3505)
                     /locus_tag="PKJ43_03135"
     CDS             complement(<1..>3505)
                     /locus_tag="PKJ43_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNZ71585.1"
                     /translation="KDLLVRNAERLKELKGINATSEILKKGLPIQQSLSELVDILPAS
                     WQYVEHTVARISYGDLVFESQQFEESPWRMAQEFESPKKQKGLIEIFYTRQFPDEDEG
                     PFLKEERNLLINIANLISGIATREVFNKLQHDNTERLKELRAINQTSAIIEDGKPVDE
                     TLQAICNILPQSWQYPKCTVARIRFDGKNYVSKNFKETIWGQKEYFITIDNKKGLIEI
                     FYTKEFPFEYEGPFLAEERNLLVNIGKLISGYLNNYKGREIYHKNVFMSNQMHKAEEY
                     RKSLIRNKKPLQLYFNQQTIDKYVYLDMMKYKIKHILFVATLYDAFVLENEDSFFEKF
                     MGEIYQYSLFSLPRITGVSTAEEALEVLETTQFDLVILMAGLDREAQIILSEKIKQQN
                     ESLPVYLLLNKKSDMRYYEELVPTIGSVDKMFVWNGDSSIFFSIVKSIEDKVNVENDT
                     KIGLVRVILLIEDSPLYYSKYLQILYGIVFGQVQQLLPEVEKNELDKICKMRSRPKIL
                     HARNWDEAMMIFDKYQDFMLCVISDVEFERSGKMDINAGVKFIKYVKSKISDLPIILQ
                     SSEDENENLAKRLKVKFINKNSETLLTDIRKYLISYLGFGNFTFRDKEGNKIGVAKSL
                     RDFETLLRDVPDESFYVHAHQNQFSLWLMARGEIQLAKTMNSQLVDENTDLRLARNEF
                     LEAIQAYREVKKKGKILQFDETSTLDEKNIVSFASGSLGGKGRGLAFINTLINNIEFP
                     ELNGKINILAPITVIIGTDEFQSFISRNKLMPYIIDSDVSFEKLREIFAQGSLSHSLE
                     MKLKDFVSQIDKPIAVRSSSISEDSLTQPFAGVFDTYIVPNGDSNKRLVLENIETAIK
                     LVFASIYSDKARLYFRAINHKIEDEKMAVVIQELVGSKYGKHYYPHISGVAQSYNYYP
                     IGHMKPEEGFAVAAVGLGSYVVDGWKAFRFSPVYPRLSMYTVKDLLNSTQVQFFALDC
                     EQNNIDFLKDGEFASLKLLDISVAENDGALKHCVSVYNRDNDRVEPGLSARGPRIVNF
                     ADILQFNHIPLAQTISLLLNAVKEAFGSPVEIEWAVNLDRTKNNLPTFYLLQIKPLVG
                     TQFFSHIDINAIDKNKMLLFTRSSLGNGEVKGICDVIYIDREKFSKLKTLEMVNEIEY
                     LNNIMVKENRNYVLIGPGRWGT"
BASE COUNT         1053 a          733 c          585 g         1134 t
ORIGIN      
        1 ggtcccccaa cgacctggtc cgataagtac gtaattcctg ttttctttta ccataatatt
       61 attcaggtat tcgatctcat taaccatttc aagggttttc aacttgctga atttttcccg
      121 gtcaatataa atcacgtcac aaatcccttt tacttcacca ttaccaaggc tagaacgggt
      181 aaacaacagc attttattct tatctatagc attgatatca atatggctaa agaactgagt
      241 accaaccaat ggcttaattt gtaaaagata aaacgttggt aagttatttt ttgttctgtc
      301 gaggttaact gcccattcaa tttcaacagg cgaaccaaaa gcctctttaa cagcatttaa
      361 caataaggaa attgtttggg ctaagggaat atggttaaac tgtaaaatgt cggcaaaatt
      421 aacaatacgt ggcccccgcg cacttaatcc tggctctacc ctatcgttgt cacggttgta
      481 aacagataca caatgtttta gcgcaccatc attttcggca acacttatgt ctaaaagttt
      541 taaagaagca aattccccat ctttcaaaaa gtcgatatta ttctgttcac aatcgagtgc
      601 aaaaaactga acctgagtac tattcagcaa gtctttaact gtatacatcg ataagcgagg
      661 atatacagga gaaaagcgga aagctttcca cccatcaacc acatatgatc ccagccccac
      721 agctgctact gcaaatccct cttcaggttt catatgccct atagggtaat agttatacga
      781 ttgggcaacc ccacttatat gtggatagta atgttttcca tacttacttc caacaagttc
      841 ctgaataaca acagccattt tttcatcttc aattttatga tttatggcac ggaaatatag
      901 tcgtgcttta tcagaataaa ttgaagcgaa aaccaattta atagctgttt caatattttc
      961 aagaaccaac cgtttattgc tgtcgccatt gggtacaata tacgtatcga aaacacctgc
     1021 aaatggctgt gtaagtgagt cttcagaaat acttgacgaa cgaacagcaa ttggtttatc
     1081 aatttgcgaa acaaaatctt taagcttcat ttcgagggaa tgagacaatg atccttgcgc
     1141 aaatatttcc ctaagctttt caaacgaaac atcggaatcg attatgtaag gcatcagctt
     1201 atttcgcgaa atgaaactct gaaattcatc ggtaccaata atgactgtaa ttggagccag
     1261 gatgttgatt tttccgttta attcgggaaa ttctatattg ttaatcaggg tattaataaa
     1321 tgccaatcca cgtcctttac cccccaatga accactggca aacgatacta tatttttctc
     1381 atcaagggtc gaagtctcat caaactgaag gatttttcct tttttcttta cttctcgata
     1441 tgcttgaata gcttcaagaa attcgttgcg agccaacctc agatcagtat tttcatcaac
     1501 caattgagaa ttcatcgttt ttgccagctg tatttcgccc cgagccataa gccaaagtga
     1561 gaactgattt tggtgtgcat gcacataaaa cgattcatca ggaacgtcgc gcagtaaagt
     1621 ttcaaaatcg cgtaacgact tagccacacc aattttatta ccttccttgt cgcgaaatgt
     1681 aaagtttcca aaacctaaat aactgatcag gtatttacga atatctgtca acaaggtctc
     1741 agaattttta tttataaatt taactttcag ccttttggcc agattttcat tttcatcttc
     1801 tgaagactga aggataattg gcaagtctga aattttagat tttacatatt ttataaactt
     1861 tactcctgcg ttgatatcca tctttccact tcgctcgaat tctacatcag agattacaca
     1921 aagcataaaa tcctggtatt tgtcgaatat catcatcgcc tcgtcccaat ttcgagcatg
     1981 aaggattttt ggtctcgaac gcattttgca aattttgtcg agctcgtttt tctctacctc
     2041 gggcaacagc tgctgaactt gtccgaagac tataccatat aatatctgga ggtattttga
     2101 ataataaagc ggtgaatctt caataagtaa gataaccctt accagcccaa ttttcgtatc
     2161 attttcaaca ttaactttgt cttcaataga ctttacaatg gaaaaaaata tggacgaatc
     2221 gccattccaa acaaacatct tatcgacaga accgatggtt ggcaccagtt cttcgtaata
     2281 acgcatatca cttttcttgt tcaacaacaa atataccggc aacgattcat tttgctgttt
     2341 aatcttctca cttagaatta tttgagcttc ccgatcgagg cctgccataa gaattaccag
     2401 atcaaactga gtagtttcaa gcacttcgag ggcttcctca gcagtagaga ctcctgtaat
     2461 acgaggaagc gaaaacaagc tgtactggta tatttctccc atgaattttt cgaagaagga
     2521 gtcttcgttt tccaacacaa acgcatcgta aagagtggcc acaaaaagta tgtgttttat
     2581 tttgtacttc atcatgtcga ggtacacata cttatcgatg gtttgctggt taaaatataa
     2641 ctgaagcggt tttttatttc taatcaacga cttacgatat tcctctgctt tgtgcatctg
     2701 attcgacatg aaaacatttt tgtggtatat ttcacgccct ttataattat tgagataacc
     2761 gctgatgagt ttccctatgt tgacaagcaa atttcgttct tctgcgagga atgggccttc
     2821 atactcgaat ggaaattctt tagtataaaa aatttcaatc aagccctttt tattgtcgat
     2881 ggtaataaaa tattcctttt ggccccaaat cgtttctttg aaatttttac tcacataatt
     2941 tttaccatcg aaacgtattc gggcaacagt acatttaggg tactgccacg attgtggtaa
     3001 aatattgcaa atagcctgca aggtttcatc aactggtttt ccgtcttcaa ttattgccga
     3061 agtttgatta attgcccgca attctttaag tctctcggta ttatcgtgtt gtaatttatt
     3121 gaaaacttcg cgagtagcta tacccgaaat aagattcgct atattaatga gcaagttacg
     3181 ttcttccttt aaaaacggcc cttcgtcttc atcggggaac tgccgggtat aaaaaatttc
     3241 tatgaggcct ttttgttttt tcggactctc aaattcttga gccatccgcc agggactttc
     3301 ttcaaattgt tggctctcga atactaaatc accataacta atgcgggcaa cagtatgctc
     3361 aacatactgc cacgaagctg ggagtatatc caccaactca ctcaaagact gctgtatagg
     3421 cagtcctttt tttaatattt cagaagtagc atttattcct ttcagttctt ttaaccgttc
     3481 ggcatttcga acaagcagat ctttc
//
LOCUS       DAOPUW010000127         3492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_27437_length_3492_cov_3.272040, whole genome shotgun sequence.
ACCESSION   DAOPUW010000127 DAOPUW010000000
VERSION     DAOPUW010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27437_length_3492_cov_3.272040"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="PKJ43_03140"
     CDS             <1..1056
                     /locus_tag="PKJ43_03140"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71586.1"
                     /translation="PMNTSTSRKKKKISSFLLLGLTLGAFVLAGFSPTQKPSARIMFY
                     NVENLFDTIDNPDKSDEEFLPDGVRNWGGYRFYIKLQRLSQVIVATGENGFPAVIGMC
                     EVENHQVLESLIHHTPLKKIDYGIVHQESPDSRGIDVAMLYRKDMFSPQFFRAIRVSD
                     PADPSFTTRDILYVKGLLGTDTMHFFVNHWPSKYGGVSATKPRRALAASVLRAQTDSL
                     LARNPSANIIIMGDLNDSPLDKSVAGVLGAKPVGKPSPSGLYNLAYPLAKKGIGTNKY
                     RSKWDLIDQFIVSRNLVSKDGMASHSDAFSVFSPSYLLVKDENNLGEKLNRTFVGVKY
                     NGGFSDHLPILLDLRKK"
     gene            complement(1136..>3492)
                     /locus_tag="PKJ43_03145"
     CDS             complement(1136..>3492)
                     /locus_tag="PKJ43_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HNZ71587.1"
                     /translation="QWIDKTDWEYSTTFVVSNDENNKENQELCFKGLDTYADVYLNDS
                     LILQTDNMFRNWKIAVKGIAHEGENQLRLLLHSPINRGLAFLEKSAYPYPAANDQSEN
                     GGLGDKKVSIFTRKAGYHYGWDWGPRLVTSGIWRPVYFNAWDSGRIENVFIMQPDVTA
                     QKASLVASVTFSANKESDVEVTIYQDNKKITSKKTKAIPGENQLELPFTISKPKLWWS
                     NGLGEANLYTFTTQLKINGNVFHSISTQTGIKSLKLVRTPDDKGESFYFELNGVPVFA
                     KGANYIPNDNFLPRVSRQQYEKVISDAVDANMNMLRVWGGGIYENDYFYELCDEQGIM
                     VWQDFMFACSMYPGNEAFLKSVAAEAKDNIIRLRNHPSIVLWCGNNEIDAAWQYSNEH
                     GGWGWKQRYSTQQRDEIQQAHLAIFHDILPSAVEKYTDGDYYWPSSPMSGDKPNQHSN
                     YTSGDVHYWGVWHGLHKFSEFETNIGRFMSEYGFQSFPAFETVKKYALPEDYSIESEV
                     MAAHQRSGIGNLRIREYMSWDYNVPTDFEQFLYVGQLLQSRGIKAAIEAHRRAMPFCM
                     GSLYWQINDCWPVASWSSTDYYHNWKAMHYMARKAFMPVIISVFEKEGQITFTAASDQ
                     LQNIDTELEIKIIDFAGTELSKMTVPAQINANSATLLKTISKQELTGNSNPAHVALVA
                     SLKSNGKTLADNIFYFVPPKDLVLEKPALKVSVSEENGDKIIRLKSETLVKDIMLYVP
                     DAQLAFIDNYFDMLPNTEYGVSVKTTMSAKELSEKLKFRDLSMK"
BASE COUNT          879 a          746 c          784 g         1083 t
ORIGIN      
        1 cctatgaata catctacatc gagaaaaaag aagaagatta gtagttttct cctcctgggg
       61 ctcaccttag gggcctttgt gttggccggt ttttctccaa cccaaaagcc ttccgcacga
      121 attatgttct ataatgttga aaatcttttt gatactattg ataatcccga caaaagtgat
      181 gaggaatttc tgccagatgg tgttcgaaat tggggaggtt accgctttta cataaaattg
      241 cagcgcctgt cgcaggtgat agtggctact ggcgaaaatg gatttcctgc agttataggc
      301 atgtgtgagg ttgaaaacca tcaggttctt gagtcattga ttcatcatac cccgctcaaa
      361 aaaatcgatt atggcattgt tcaccaagag tcgcccgatt cgcggggaat tgatgtggcc
      421 atgctatatc gtaaagatat gttttcgcct caatttttca gggcgatcag ggtttcagac
      481 cctgccgatc cttcttttac cacgcgtgat attttatatg tgaaaggcct gcttggtact
      541 gacaccatgc atttttttgt taaccattgg ccatcaaaat acgggggagt gagtgcaacg
      601 aagcctcgca gggcgttggc tgcttcggtg ttaagggcac aaaccgacag tctgcttgct
      661 cgcaacccgt cagccaacat tataattatg ggcgatctga acgattctcc tctcgacaaa
      721 agtgttgcag gggtgcttgg tgcaaagcct gtggggaaac catctccgtc gggtttgtat
      781 aatttggcat atcccttggc taaaaaagga attgggacca ataaatatcg ctccaaatgg
      841 gatctcatcg accagtttat cgtgagccgg aatcttgtgt cgaaagatgg aatggcctct
      901 cattctgatg ccttctctgt tttttcacca tcctatttat tggtcaaaga tgagaataac
      961 ctgggcgaaa aacttaaccg aacttttgta ggtgtaaaat ataacggtgg ttttagtgac
     1021 catttgccca tattactcga tttgagaaaa aaataacaac ctttcaacct caaaaaaacc
     1081 atggatttgt acattaaaga tgtaaatcaa tggccctaaa aaagggagag cctgtttact
     1141 tcattgacaa atccctgaat tttagcttct ccgaaagttc cttggcactc attgtggttt
     1201 taacactgac accatattcg gtgttgggca gcatatcaaa gtagttgtcg ataaaggcta
     1261 attgcgcatc ggggacatat agcataatgt cttttaccag tgtttcgctt ttcagtctga
     1321 ttattttatc gccattttct tccgaaacag atactttcag cgctggtttt tccagcacca
     1381 gatctttggg cgggacgaag tagaagatgt tatctgccag ggttttccca tttgacttta
     1441 acgaggcgac aagggctacg tgcgccgggt tactgttgcc ggtcaattct tgtttcgata
     1501 ttgtttttag cagggtagca ctgttggcgt ttatttgtgc cggaactgtc attttgctca
     1561 attcggttcc tgcaaaatca atgattttaa tttcaagttc agtatcaatg ttttgtagtt
     1621 ggtctgatgc tgcggtaaag gtaatttgac cttctttttc aaaaactgat ataataactg
     1681 gcataaaggc ttttcgggcc atgtagtgca tcgctttcca gttgtggtaa tagtcggtgc
     1741 tcgaccagct agccactggc cagcagtcgt ttatttgcca gtaaagagat cccatgcaga
     1801 aaggcattgc acggcggtgc gcttcaattg ctgcttttat tccgcgcgat tgcagtagtt
     1861 ggccaacata cagaaactgt tcaaaatcag tgggcacatt ataatcccag ctcatatatt
     1921 cccttatgcg aaggttgcca attccacttc gttggtgggc agccattact tccgattcga
     1981 ttgaataatc ctcgggcaat gcatattttt taacggtttc gaacgctgga aacgactgga
     2041 acccgtattc gctcataaaa cgtccaatgt tggtttcgaa ttcgctgaat ttatgaaggc
     2101 catgccatac gccccagtag tgtacatcgc ccgaagtgta attcgagtgc tggttgggtt
     2161 tgtcgcccga catcggagac gagggccaat aataatcgcc atctgtatat ttttctaccg
     2221 cactaggcag aatgtcgtga aaaatagcta gatgtgcttg ttgaatttcg tcgcgttgtt
     2281 gcgttgagta tcgttgtttc caaccccaac ctccatgttc gttgctgtac tgccaagccg
     2341 catctatttc gttgtttccg caccataaca ctatgctggg gtggttgcgc aaccttatga
     2401 tattgtcttt ggcttcggca gctacgctct tcaaaaatgc ctcgttgccg gggtacattg
     2461 aacaagcaaa cataaagtct tgccaaacca taatgccctg ttcgtcacag agttcgtaaa
     2521 agtagtcgtt ttcatagatt ccgccacccc aaacccgcag catgttcatg ttggcatcaa
     2581 cggcatctga aataactttt tcgtactgtt gccggcttac gcgggggaga aaattgtcgt
     2641 tggggatata gtttgcccct ttggcaaata ctggcacccc gtttagttcg aaatagaacg
     2701 attcaccttt gtcgtcgggg gtgcgtacaa gtttcaggct ttttattcca gtttgtgttg
     2761 aaattgaatg aaaaacgttg ccatttatct tcagttgtgt agtgaacgtg tagaggtttg
     2821 cttcgcctaa tccgttcgac caccatagtt taggcttcga aatagtaaac ggaagttcaa
     2881 gttggttttc tccagggatg gcctttgttt tcttgcttgt aattttttta ttgtcttgat
     2941 atattgtgac ttcaacatct gattctttgt ttgcactgaa tgtcaccgaa gcaacaagtg
     3001 atgctttctg agcagtaaca tcgggttgca tgataaaaac attttcaatt cggcccgaat
     3061 cccaggcatt gaaataaaca ggccgccaga tgcccgatgt aaccaatcgt ggaccccagt
     3121 cccaaccata atgatatcct gcttttcgag tgaaaattga aactttctta tcaccaaggc
     3181 ctccgttttc cgattgatcg ttggcggcag gatacgggta agcagatttt tcaagaaaag
     3241 ctaatccacg gttaatgggc gagtgaagca acaggcggag ttgattttct ccttcgtgtg
     3301 caataccttt tacagcaatt ttccaattcc tgaacatatt atcggtttgc aaaataagcg
     3361 aatcgttcag gtaaacatcg gcataggtat cgaggccctt aaagcaaagt tcctgatttt
     3421 ctttgttgtt ttcgtcgttg cttaccacga atgtggtgct gtattcccag tcggttttgt
     3481 cgatccattg ta
//
LOCUS       DAOPUW010000128         3479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_27568_length_3479_cov_4.594042, whole genome shotgun sequence.
ACCESSION   DAOPUW010000128 DAOPUW010000000
VERSION     DAOPUW010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27568_length_3479_cov_4.594042"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PKJ43_03150"
     CDS             <1..211
                     /locus_tag="PKJ43_03150"
                     /inference="COORDINATES: protein motif:HMM:NF036996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-asparaginase 1"
                     /protein_id="HNZ71588.1"
                     /translation="LNISQCAGGSVKMGRYETSRNLLDAGVLSGYDMTTEAAITKMMI
                     LLGQNRHPEEIKKLFNKNLRGEITL"
     gene            374..461
                     /locus_tag="PKJ43_03155"
     tRNA            374..461
                     /locus_tag="PKJ43_03155"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:408..410,aa:Ser,seq:gga)
     gene            521..1333
                     /locus_tag="PKJ43_03160"
     CDS             521..1333
                     /locus_tag="PKJ43_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNZ71589.1"
                     /translation="MKKLFALIAVLTVFVLGSSVSVKAQETEAPATEQVAAAQPAADV
                     AADKIENAAKAAEEGQSLHSALKQKFIEGGPGFMTSILICLILGLALAIERVIYLNLA
                     TTNTKKLLTDVEQALNKGGFEAAKEVCRNTRGPVASIFYQGLDRHNEGVEVIEKSIIS
                     YGGVQMGLLERNLSWIGLFIALGPMLGFLGTVIGMIQAFDAIEVAGDISPTLVAAGIK
                     VALITTVFGLIVAMILQIFYNYCLSKIDSITGDMEESSISLLDMIVKYNIKK"
     gene            1341..1808
                     /locus_tag="PKJ43_03165"
     CDS             1341..1808
                     /locus_tag="PKJ43_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71590.1"
                     /translation="MEKGTKIAKFVLWGLFAISIVLFILLLTSIETQENPGAKAESLI
                     TFSIYWSEILIAIGAFVALFYAAKQMVADKKQAITSLLIIAAFALIVVISYAVSSSEI
                     PKFFGVEKFVADGSLTPSISRWIGTGLVTTYILFAGAALSVIGFSAAKFFKRS"
     gene            1838..2425
                     /locus_tag="PKJ43_03170"
     CDS             1838..2425
                     /locus_tag="PKJ43_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNZ71591.1"
                     /translation="MPRKMPEIPSASLADIAFLLLIFFLVTTTMDQDKGLERRLPQWV
                     PEDQMDQDAPPIKERNIFVVLIDKENRLMVEGEICRVEDLKEKAKLFLTNPLDLPNMP
                     ETEVREFDIVGNYKVISKSAVISLRNDVGTTYGAYIQVQNELVAAINELRQELAMDKF
                     GKSYVNLSTDEQKIIQDIYPQKISEAEPKRIGGAK"
     gene            2425..2892
                     /locus_tag="PKJ43_03175"
     CDS             2425..2892
                     /locus_tag="PKJ43_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010421387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNZ71592.1"
                     /translation="MSKFKKDNTKELPAISTASLPDIVFMLLFFFMSTTSMRQNTLMV
                     KIHLPEATEINKMEKKSLVSYVYVGVPVPAMQHIYGSEPHIQLNDKFAKVTEIPDFIE
                     AERQARDESEIPQMITSLKVDEFTKMGIVNEIKQELRKAAALHINYSSRKAEK"
     gene            complement(2971..>3479)
                     /locus_tag="PKJ43_03180"
     CDS             complement(2971..>3479)
                     /locus_tag="PKJ43_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HNZ71593.1"
                     /translation="LNFMGNEFGHPEWIDFPREGNGWSYRHARRLWSIVDNHELKFHW
                     MNDFDRDMIALIRKKQLLGVPEIWKVWENRSDQVLAFTRGDLLFVFNFNPQQSYVDYG
                     IPFEPSKFKVVLTTDSGIYGGWDRVDERVAYYSMPSSGLESQHYLKLYLPARSAVVFQ
                     KEKIKRIK"
BASE COUNT         1107 a          687 c          704 g          981 t
ORIGIN      
        1 actcaacatt agccaatgcg caggaggcag tgtgaaaatg gggcgatatg aaacaagccg
       61 aaacctactt gatgccggag tattaagcgg ttatgacatg acaactgaag cagctataac
      121 caaaatgatg atcctgttag ggcaaaaccg acaccccgaa gaaataaaaa aattgttcaa
      181 caaaaacctt cggggagaaa taacattata ggtttgttta ttattctatt ttttttgtaa
      241 ttttcagccc gcaaattttg cttggtaatg tttaggtaac attattgaaa aaaatgtaat
      301 cagtttgaat aatgcgttca ttgttgagtt taaaaaaaag cactcttgca aaaatgtttg
      361 agtaaaaaaa tctggagaga tgcaggagtg gcttatctgg cacgcctgga aagcgtgtgt
      421 actccaaaag ggtaccgggg gttcgaatcc ccctctctcc gcacaaaaag aattaataaa
      481 ttaaaaatca gaatattgtt gaataaattt taaaaacatc atgaaaaaat tatttgcatt
      541 aatagctgta cttacagtgt tcgttctggg atcatcggta agcgtgaaag ctcaggaaac
      601 tgaagctcct gccaccgagc aggtagctgc tgcacaacct gctgccgatg ttgctgctga
      661 caaaattgaa aatgctgcca aagcagcaga agaaggccaa tcgttgcaca gtgcattgaa
      721 acaaaagttc attgaagggg gccctggatt catgacatcg atccttatct gtcttattct
      781 tgggttagca ttggctatcg aacgtgttat ctatcttaac cttgcaacta ccaacaccaa
      841 aaaattgttg accgatgttg aacaggcact taacaagggc ggttttgaag cagcaaaaga
      901 agtttgccgc aacaccagag gcccagttgc cagcattttc taccaaggtc tcgaccgtca
      961 caacgaaggt gttgaagtaa ttgaaaaatc gattatttcg tacggtggag ttcagatggg
     1021 cttacttgaa agaaacttaa gctggattgg attgttcatc gccttaggcc ctatgctcgg
     1081 gttcttggga acagtaatcg gtatgattca ggcttttgat gctattgaag ttgccggtga
     1141 cattagcccg actctcgttg ctgctggtat caaagttgct cttatcacaa ccgtattcgg
     1201 tcttatcgtg gctatgatcc ttcagatttt ctataactac tgcttatcaa aaatcgacag
     1261 cattaccggc gacatggaag aatcgtcaat ctcattgctc gacatgatcg ttaagtataa
     1321 cattaaaaaa taattgagcg atggagaaag gtacaaaaat agccaaattt gtactctggg
     1381 gattgtttgc tatttcaatt gttctgttta tcttattgtt aacatcaatt gaaacccagg
     1441 aaaacccagg ggcaaaggct gaaagtctaa ttactttcag catatattgg tctgaaatac
     1501 taattgcaat tggagcattt gttgcattat tttatgctgc aaagcaaatg gtagccgaca
     1561 aaaaacaagc aataacatca cttttgatca tagcagcatt cgctttgatc gtggttatat
     1621 catatgctgt ttcatcgagt gaaatcccaa aatttttcgg ggttgaaaaa ttcgtggccg
     1681 acggctcatt aactccatct atttcgagat ggattggaac tggccttgta accacttaca
     1741 ttctttttgc tggagcagcg ttgtctgtaa ttggatttag cgctgccaaa ttttttaaac
     1801 gttcatgatg tttcaacatc attaaattga gagaattatg ccaaggaaaa tgcccgaaat
     1861 accttctgct tcgttagccg acatcgcatt cttgttgtta atcttctttt tggttacaac
     1921 aactatggat caggataaag ggcttgaaag aaggttgcct caatgggtac cggaggatca
     1981 aatggatcaa gatgctccgc ccattaagga acggaatatc ttcgtagtgc ttatcgataa
     2041 ggaaaacagg ctcatggtag aaggtgaaat ttgccgggta gaagacttaa aagaaaaagc
     2101 taagctcttc ttaaccaatc cgctcgattt acctaatatg cctgagacgg aagtacgtga
     2161 attcgatatt gttggaaact ataaagttat ttcgaaaagt gcagtaatat cgttacgtaa
     2221 cgatgtggga acaacgtatg gtgcttacat tcaggtacaa aacgaactgg ttgctgccat
     2281 taacgaactg cgacaagaac tcgcgatgga taaatttgga aaatcgtatg ttaatctttc
     2341 aactgatgaa cagaaaatta tccaagacat ttatcctcag aaaatttcag aagctgaacc
     2401 aaaacggata ggaggagcaa agtaatgtcg aaatttaaga aagataatac caaagaatta
     2461 ccagcaattt ctactgcttc attgcccgat atcgtattca tgttgttgtt ctttttcatg
     2521 tcgacgactt caatgcgtca gaatacgctt atggtgaaaa ttcacttgcc tgaagcaact
     2581 gaaatcaaca aaatggagaa aaaatcgttg gttagctatg tctacgttgg tgttcctgtt
     2641 cctgccatgc aacacatata tggtagcgaa cctcacatcc agttgaatga caagtttgct
     2701 aaagttaccg aaatacccga ctttattgaa gccgaacgtc aagctcgtga cgaatcggaa
     2761 attccacaga tgattacatc gctaaaagtg gatgaattca ccaagatggg tatcgtgaac
     2821 gaaataaagc aggaactgcg aaaagctgca gcattgcaca taaactactc ttcgcgaaaa
     2881 gctgaaaaat agaaataaaa aaggaggcca cggcctcctt ttttattata tcagcttcga
     2941 aatgtccatt gatgttttat ttcaatccca ttatttaatc ctttttattt tctctttttg
     3001 aaacacaacg gcactacgtg ccggaagata tagtttcaaa taatgctggc tttcaagccc
     3061 cgacgaaggc atggaataat acgcaaccct ttcgtcaacc ctgtcccagc cgccatatat
     3121 gccagaatcg gtagtaagca caaccttaaa cttcgacggt tcaaaaggaa ttccgtaatc
     3181 gacatacgat tgttgtgggt taaaattaaa gacaaacaat aaatcaccac gggtaaatgc
     3241 caatacctga tcggatcggt tttcccaaac tttccatatt tcaggtaccc ccagcaactg
     3301 tttcttccta atcaatgcaa tcatatcccg atcgaaatca ttcatccaat gaaacttaag
     3361 ttcatggtta tcgacaatac tccaaagccg acgggcgtgt ctgtacgacc aaccgttgcc
     3421 ttccctcggg aaatcaatcc actcgggatg accaaattcg ttacccataa aattcaaat
//
LOCUS       DAOPUW010000129         3456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_27823_length_3456_cov_3.136136, whole genome shotgun sequence.
ACCESSION   DAOPUW010000129 DAOPUW010000000
VERSION     DAOPUW010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27823_length_3456_cov_3.136136"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PKJ43_03185"
     CDS             complement(<1..702)
                     /locus_tag="PKJ43_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNZ71594.1"
                     /translation="MKPTLVILAAGMGSRYGGLKQLDEVGPNGEAIIDYSLFDAIRAG
                     FGKVVFIIRRDFEDAFKERFDKKLKGKINVEYVFQELTDLPAGFSVPEGREKPWGTGH
                     AIRAARNVVKEPFSAINADDFYGFEAYDTVARFLTGDVDDNQYAMVGYRLDKTLSENG
                     SVSRGLCVTNAQQNLVDIEELTQITNETEGIFYYKDNEKVGLKGYETVSMNFWGFHPS
                     LFTHLEEGFVSFLKEK"
     gene            1045..1395
                     /gene="rpsF"
                     /locus_tag="PKJ43_03190"
     CDS             1045..1395
                     /gene="rpsF"
                     /locus_tag="PKJ43_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNZ71595.1"
                     /translation="MLNQYETVFIATPVLSDAQFSEVVAKFSGIISSNNGEVIHQEDW
                     GLKKLAYPIQKKSTGFYHLIEFKAEPTFIEKLETEYRRDERIIRFLTFKLDKHAVEYS
                     EKRKSKVKAKAEEK"
     gene            1399..1668
                     /gene="rpsR"
                     /locus_tag="PKJ43_03195"
     CDS             1399..1668
                     /gene="rpsR"
                     /locus_tag="PKJ43_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNZ71596.1"
                     /translation="MASNQSEIRYLTPPSVEVKKKKYCRFKKNGIKYVDYKDPEFLKK
                     FLNEQGKILPRRITGTSLKFQRKVSQAVKRARHIALLPYVTDLMK"
     gene            1684..2130
                     /gene="rplI"
                     /locus_tag="PKJ43_03200"
     CDS             1684..2130
                     /gene="rplI"
                     /locus_tag="PKJ43_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNZ71597.1"
                     /translation="MEIILKQDVQGLGSKDDILKVKDGYGRNYLIPQGMAIVATESAK
                     KVLAENIRQRAHKEAKLKEEASVLAERIKALTVTIGAKTSTTGKIFGSVNNIQLAEAI
                     AAQGIEIDRKQIYIAKEGVKEIGKHIAKIKLHREVTVDFEFEVVSE"
     gene            complement(2194..2571)
                     /locus_tag="PKJ43_03205"
     CDS             complement(2194..2571)
                     /locus_tag="PKJ43_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNZ71598.1"
                     /translation="MKRIISTEKAPAAIGPYSQGVEVNGTLYISGQVPIDPETGSLVE
                     GDITVQTERVMKNIGAILDSAGYSFADVVKSTCLLSDMDNFKAMNSVYATFYPQNPPA
                     RAAFAVKGLPLGALIEIETIAAK"
     gene            complement(2703..>3456)
                     /locus_tag="PKJ43_03210"
     CDS             complement(2703..>3456)
                     /locus_tag="PKJ43_03210"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HNZ71599.1"
                     /translation="EMKLRNLSDTTGKEAFKKVPIFDDLSFGGNYNLAADTNRLSNIS
                     LNLRTKIAGTVLNISGNLNPYALDSLGRVTRFYMWEKASGIAKLGRITDLGTGFSYNF
                     GSSQIEKWRKEREAKKGGSEAKKSETETVSSNYQSFKNSWSIRYNFNIRYSNPAKNPK
                     IRITQTLTFDGSIDITNKWKANVTSGFDFNAMKLSHTNFSVTRDLHCWLMTFSFSPIS
                     QNQYYMFTLSANSAMLKDLKIDKRSTSMYANY"
BASE COUNT         1121 a          704 c          624 g         1007 t
ORIGIN      
        1 tttttctttc agaaaagaaa caaatccttc ttccaaatgc gtgaaaagcg atggatgaaa
       61 tccccaaaaa ttcattgaaa ctgtctcgta gccttttaac ccaacttttt cattgtcttt
      121 ataatagaaa atcccttctg tttcatttgt gatttgagtc agctcttcaa tgtcgacaag
      181 gttttgctgt gcattggtaa cacaaagtcc gcgcgaaacc gaaccattct ctgatagggt
      241 tttatcgagc ctgtacccaa ccatcgcata ttggttatca tccacatcgc cagtaaggaa
      301 gcgggcaact gtatcataag cctcgaagcc ataaaaatcg tcggcattaa tagcggagaa
      361 aggttctttc actacatttc gtgcggcacg tattgcatga ccggttcccc aaggtttttc
      421 acggccttca gggactgaaa aacctgctgg caaatcggta agttcctgaa aaacatattc
      481 aacattgatt ttacctttca attttttatc aaaacgctct ttaaaagcgt cctcaaaatc
      541 gcggcgaata atgaaaacaa ccttgccgaa gcctgctcta atggcatcaa ataatgaata
      601 atcgataatt gcttcaccgt tggggccaac ttcatcaagt tgttttaagc caccgtagcg
      661 gctacccatt ccggcagcca aaattactaa tgtaggtttc atttagataa tataattaac
      721 gggttattga atcgcagcat attaaattga aaattatcaa ttgcgggcaa aaataaaaca
      781 tttgaaattt gataacgagt ttatgccaca aaatgactat aaaaagaaaa ggttttaaaa
      841 cccaaaagat taaagaacaa cgaaaaaatg aaaatttgga ggcaaggttt agttttttca
      901 ccgattaatt tgctgcgacg caaataattc ctacttttgc gccgctcaat tcgctgaatc
      961 catgtaaccg attatttacc ggtcgattac gcgaatagag caaataaata aacaattaat
     1021 aaacaattaa aaggtaaacg ttctatgtta aaccagtacg agactgtatt tattgctacg
     1081 cctgtattgt ctgatgcaca attcagtgaa gtagtagcaa aattcagcgg aattatttcg
     1141 tcgaataatg gcgaggtaat ccaccaggag gactgggggc taaaaaaatt ggcttaccca
     1201 atccagaaaa aatcaacagg tttttatcac cttatcgagt ttaaagctga gcctacgttc
     1261 attgaaaagc ttgaaaccga ataccgtcgt gacgagcgca ttattcgttt tctgactttc
     1321 aaattggaca agcacgctgt tgaatacagc gaaaaacgca aaagtaaagt taaagctaaa
     1381 gcagaggaga aataagccat ggcatcaaat caatcagaaa tcagatactt gaccccaccg
     1441 tcggtagagg ttaaaaagaa aaagtattgc cgttttaaaa agaacggaat caagtatgtc
     1501 gattacaaag accctgaatt cttgaagaaa ttcctgaacg aacaaggtaa aattttaccc
     1561 cgtcgtatca ctggtacttc actaaaattc cagcgtaaag tatctcaggc agttaaacgt
     1621 gcccgtcata ttgctttatt gccatacgta accgacttaa tgaaataatc tggaggattc
     1681 ttaatggaaa tcatattaaa acaagacgtt caagggctag gctctaaaga cgatatcctc
     1741 aaagttaagg atggttatgg ccgcaactac ctcattcctc agggaatggc cattgttgct
     1801 accgaatcgg caaagaaagt gctcgctgaa aacattcgcc agcgtgccca caaagaagca
     1861 aaactgaaag aagaagcttc tgttcttgct gaaagaatca aagcacttac tgttactatt
     1921 ggtgcaaaaa ccagtacaac aggtaaaatc ttcggatcgg ttaacaacat tcaacttgcc
     1981 gaagctattg cagcacaagg aattgaaatt gaccgcaaac aaatctacat tgctaaagaa
     2041 ggtgtaaaag aaattggcaa acacattgct aagatcaaac tccatcgtga agtaactgtt
     2101 gatttcgaat tcgaagttgt atcagaataa cacacacctt ataaatgaaa aggctacctt
     2161 ttgggtagcc ttttttttcg cataattttc aaattatttt gcagcgatag tttcaatttc
     2221 gattaaagca cctaacggca atcctttcac ggcaaaagca gctcttgccg gagggttctg
     2281 aggataaaaa gttgcatata cactgttcat cgctttgaaa ttatccatat cgctcaataa
     2341 acatgtcgat tttaccacat cggcaaaact atatcctgcc gaatcaagaa tggcaccaat
     2401 gttcttcatt acccgttctg tttgaacggt aatatctcct tcgaccaaac ttccagtctc
     2461 cgggtcgata ggaacctgcc ccgaaatata cagagttccg ttaacttcaa cgccttggct
     2521 ataagggcca atggcagccg gagctttctc tgtactaata attcttttca ttttgctata
     2581 tcttctgagt taatattttg attaagtaac aaataacaac actttccatt tatcctatat
     2641 tttgccatat tgtaaacctt aacatgacca gttgtggcaa gaaccaaata tgcaacaaat
     2701 tattaataat ttgcatacat agaagtgctc cgtttgtcaa ttttcaggtc tttcagcatt
     2761 gctgaatttg ctgagagcgt gaacatataa tactggtttt gtgaaatagg tgaaaagctg
     2821 aaagtcatta accaacagtg aaggtcccgg gtaacagaaa aattcgtgtg cgataatttc
     2881 atcgcattaa agtcgaatcc cgaagtgacg ttagctttcc acttattggt tatatcaata
     2941 ctcccgtcaa aggtaagtgt ttgggtgatt ctgatttttg gattttttgc tgggttagaa
     3001 taacgaatat taaaattata acgaatactc cacgaatttt taaaagactg ataattagaa
     3061 cttacagttt ctgtctcaga tttttttgct tcacttcccc ccttctttgc ttcacgttct
     3121 ttacgccact tttcaatttg actcgagcca aagttatagc taaaaccagt tcccaaatcg
     3181 gtaatacgcc ccaactttgc tatacccgat gctttttccc acatataaaa ccgggtaacc
     3241 ctccccaagc tgtcaagggc gtaaggattt aaatttcctg aaatgtttaa tactgtcccg
     3301 gcgatttttg tccttaaatt taaactaatg ttagacaaac ggttcgtatc ggctgccagg
     3361 ttataatttc caccaaacga aagatcgtcg aaaatgggaa cttttttaaa ggcttctttg
     3421 ccggtagtat cacttaggtt gcgcaatttc atttca
//
LOCUS       DAOPUW010000130         3435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_28048_length_3435_cov_2.875740, whole genome shotgun sequence.
ACCESSION   DAOPUW010000130 DAOPUW010000000
VERSION     DAOPUW010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28048_length_3435_cov_2.875740"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PKJ43_03215"
     CDS             complement(<1..518)
                     /locus_tag="PKJ43_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNZ71600.1"
                     /translation="MSNIVAIVGRPNVGKSTLFNRLIEQRSAIVDDISGVTRDRNYGK
                     SEWNGVEFSVIDTGGYVMNSNDVFEDEIKRQVHLAIDESDVILFVVDVESGITDLDEA
                     VATILRREKKPVFIVVNKVDNNKRQSDAYEFYNLGLGELYSISSMNGSGTGDLLDKVV
                     EAFPKDNTPEEEL"
     gene            complement(619..1503)
                     /gene="era"
                     /locus_tag="PKJ43_03220"
     CDS             complement(619..1503)
                     /gene="era"
                     /locus_tag="PKJ43_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNZ71601.1"
                     /translation="MAHKSGFVNIIGNPNVGKSTIMNALVGEKISIITSKMQTTRHRI
                     KGIVSGDDFQIVYSDTPGILKPNYKLQEEMMKFVDTALTDADVILYVTDVVETVNKNP
                     EYIEKINKSGVPVILLINKIDLSEQQKVLELVKTWKELLPGAEILTISATEKFNIAPI
                     FDMILEKLPEGPAYFPKDELTDRNERFFVQEIIREKILLYYQKEVPYSVEIEVESFKD
                     EEKLLRIQAVIHVNRETQKGIIIGHQGKAIKKVGTEARKDIEEFFEKKVFLELFVKVN
                     KDWREEERSLRNFGYEMQ"
     gene            complement(1707..2690)
                     /locus_tag="PKJ43_03225"
     CDS             complement(1707..2690)
                     /locus_tag="PKJ43_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HNZ71602.1"
                     /translation="MNSKSIIGIGNALMDIVVKLNDDTILEKNKLPKGSMTLVDSATS
                     ASLNHQTEGMEKILAAGGSVANTIDGLAHLGAPTSFIGKVGNDELGRIYYEDLKNIGV
                     KPHLFKSFNPTGVAMALVTPDSERTFGTYLGVAVEMSPNDLSSTLFEGHNIAYVEGYL
                     VQNHNLVRQAASLAKNAGLSIALDLASYNVVEENLDFLKEIASEYVDIIFANEEESKA
                     FTGKNPRESVKELSKLCHIAVVKTGAQGSLICSGKEFIEIGVTGNSCVDTTGAGDLYA
                     AGFLYGYSLGLPLEKCGKIASVVAGKVISIYGARMNETMWEQIKAEVSKII"
     gene            2794..>3435
                     /locus_tag="PKJ43_03230"
     CDS             2794..>3435
                     /locus_tag="PKJ43_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71603.1"
                     /translation="MITKSFTIRKLWLNAALLFLSFLGILSAKGQTEDCKLSIGTNLG
                     GISDWMTEMPFVDMMHNARTWGTRNQTDWIEGGVNEWNTELANRVEKDENGYPLEVPF
                     FFDDAALEDSQIIFTVWAYLAAWPSGIYTCFYEGEGEIQFGADGEVIHAEPGKLTVNI
                     TPSSEGGFLEMKITRSKRGNHIRNIRLIMPGHENTYQDQPFNPLYLEKLQPFKA"
BASE COUNT          875 a          749 c          715 g         1096 t
ORIGIN      
        1 agttcctctt caggagtgtt gtctttagga aaagcttcaa ctaccttgtc gagcaagtcg
       61 ccggttccgg agccattcat cgacgaaata ctgtagagct cacccaatcc aaggttataa
      121 aactcgtaag catcactttg ccgtttgtta ttgtcaactt tgtttaccac gataaaaaca
      181 ggtttctttt cgcggcgaag tattgtggcc acggcttcat cgaggtcggt aattccgctt
      241 tcgacatcaa caacaaaaag tattacatcc gattcgtcga tcgcaaggtg aacttgtcgt
      301 ttaatctcat cttcaaaaac atcgttcgaa ttcattacat atccgccggt gtcgattacc
      361 gagaattcaa cgccgttcca ctcgcttttg ccatagttgc ggtcgcgggt tacccccgaa
      421 atgtcgtcga caatggccga acgttgttct ataagccggt taaagagtgt cgatttgcct
      481 acattgggcc ttccaactat tgctactata ttgctcatct ttcttttctg tttttcaagg
      541 gaatcaaatg gtttccccgc agtttctata tcaatttctt attcttgccc ttgtgcttga
      601 agatatgccg ggtgcaaact attgcatttc gtaaccgaag tttcgcaaac tccgttcttc
      661 ttcacgccag tctttgttca cttttacaaa caattcgaga aatactttct tttcaaagaa
      721 ttcttcgatg tcttttcgtg cctcggtgcc tacttttttt atcgcttttc cctggtggcc
      781 gataatgatc cctttctggg tttcgcggtt tacatgtata acggcctgta tccgaaggag
      841 tttttcttca tctttgaacg actcaacttc tatttcaacc gagtaaggga cttctttttg
      901 gtagtacagt aatatttttt cgcggatgat ttcctgcaca aaaaagcgtt cgttgcggtc
      961 tgtcagctcg tcttttggaa agtatgccgg gccttcaggg agtttttcaa gtatcatgtc
     1021 gaaaatgggc gcgatattga acttctctgt agctgaaata gtcaatattt ctgccccggg
     1081 caacagttct ttccatgttt tgaccaattc aagcactttt tgctgttccg ataaatcgat
     1141 tttgtttatc aatagaatga caggaactcc cgatttattg attttttcga tgtattcagg
     1201 gtttttgttc actgtttcaa ccacgtcggt tacataaagg atcacatcgg cgtcggtgag
     1261 cgctgtgtct acaaatttca tcatttcttc ctgaagtttg tagttgggtt tcaatatccc
     1321 cggggtgtcg gaatacacga tctgaaagtc gtcgccacta acaattcctt taatgcggtg
     1381 acgggtagtc tgcattttcg acgtgataat agatattttc tcgccgacca acgcgttcat
     1441 aatggtcgat ttcccaacgt tgggattgcc tatgatattt acaaaacctg atttgtgtgc
     1501 catggtattc gatgcttttt taagcggggc aaagatagtc ttttttagtc aatgaataaa
     1561 tcgctgaaaa aaaagaggtt aaatgttaac cttctgtaag taaaaacgtt ttgagagggg
     1621 attttgctgt gaaaaaatgc ccgtcgggtt gtttttttcc ggcgggcata ctttattgaa
     1681 aacgtgtaaa cgttgattcc tgtcttttat ataatttttg agacttcagc ttttatttgc
     1741 tcccacatgg tttcattcat ccgggcgccg tatatactaa tcactttacc cgcaacgact
     1801 gaggcaattt ttccgcattt ttcgagggga agtcccagcg agtaaccata tagaaatccg
     1861 gctgcgtata ggtcgccggc ccctgtagtg tctacgcaag agtttcctgt aacgccaatt
     1921 tcgataaatt cttttcccga acagatgagc gatccttgag ccccggtttt tactactgca
     1981 atgtggcata atttcgacag ttctttcacc gattcacgtg ggtttttccc ggtaaaagct
     2041 ttcgactctt cttcgttggc aaaaatgata tcgacatatt cgctggcaat ttccttcaga
     2101 aaatcaaggt tttcttcaac cacattataa cttgccagat cgagggcaat gcttagtccg
     2161 gcattttttg ccaagctggc agcctgtcgt acaagattat gattttgaac cagataacct
     2221 tcaacatacg caatgttatg tccttcgaaa agagtagaag aaagatcatt gggcgacatc
     2281 tcaacggcta ctccaagata ggttccgaac gtgcgctccg aatcgggcgt taccaaagcc
     2341 atggctaccc ctgttgggtt aaatgatttg aaaaggtggg gtttgacccc tatattttta
     2401 aggtcttcgt aataaatccg gcctaactcg tcatttccta ctttcccgat aaatgatgtt
     2461 ggtgcgccaa gatgggccag cccgtctatt gtgtttgcta ccgaacctcc ggcagcaagt
     2521 attttttcca taccttcagt ctggtggttc agacttgccg aggtagctga gtctaccagg
     2581 gtcatacttc ctttagggag cttgtttttt tccagaatgg tgtcgtcgtt tagctttacc
     2641 acaatgtcca tcagtgcatt accaatacca attatactct ttgaattcat tgtttctgaa
     2701 ttttaatgag ctacaaagtt gtgaattttt tccactcgga cgataaatat cggaataaac
     2761 ccataaattg cattaacaaa acaacctatt actatgatta ccaaatcctt tactatacga
     2821 aaactttggc ttaatgcagc gcttttgttt ctctcttttt tgggaatact ttctgccaaa
     2881 ggtcaaacgg aagattgtaa attatcaata ggcacaaact taggtggaat ttcagactgg
     2941 atgactgaga tgccctttgt agatatgatg cacaatgccc gcacatgggg cacccgaaac
     3001 cagacagact ggattgaagg gggtgtaaat gaatggaata cagaactcgc caatcgtgtg
     3061 gaaaaggacg aaaatgggta tccgctcgaa gttccctttt ttttcgatga tgccgcgctt
     3121 gaagattcac agattatttt taccgtctgg gcttatcttg ccgcctggcc gtcagggatt
     3181 tatacctgtt tctacgaggg agaaggcgaa atacagtttg gtgccgatgg cgaagttatc
     3241 catgctgaac caggaaagct aacggttaat ataaccccgt catctgaagg gggttttctc
     3301 gaaatgaaaa ttacgcgatc gaaacgcgga aaccacatca ggaatatcag attaataatg
     3361 ccggggcacg aaaatacgta tcaggatcaa ccttttaacc ccctctattt agaaaaattg
     3421 cagccattta aggct
//
LOCUS       DAOPUW010000131         3433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_28071_length_3433_cov_2.990231, whole genome shotgun sequence.
ACCESSION   DAOPUW010000131 DAOPUW010000000
VERSION     DAOPUW010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28071_length_3433_cov_2.990231"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PKJ43_03235"
     CDS             complement(<1..316)
                     /locus_tag="PKJ43_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNZ71604.1"
                     /translation="MENQEVISMPRIGETAPEFKAVTTQGTINFPSDFKGSWVILFSH
                     PADFTPVCTSEFMTFASMENQFAEYNCKLVGLSVDGFYSHIAWLRTIKEKIEYKGMKD
                     VEV"
     gene            468..1040
                     /locus_tag="PKJ43_03240"
     CDS             468..1040
                     /locus_tag="PKJ43_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HNZ71605.1"
                     /translation="MARPKRIRKVINPPHFKGYRPLGLPEENNPVILNYEEYESIRLS
                     DFELYGQHEAAQVMEVSRPTYARIYESARRKVAQAFVSGRPIVFEGGKVYFDSEWYSC
                     TGCGCWFNHPSKDQEIKNCPLCGAPDIKQYYEDNDQSAIEDVCVCPKCGKEKKHLPDT
                     PCRKEVCPDCNSFMIRKGGPHHYRNGNKNC"
     gene            1046..1369
                     /locus_tag="PKJ43_03245"
     CDS             1046..1369
                     /locus_tag="PKJ43_03245"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNZ71606.1"
                     /translation="MKVAITSTGNSLESKLDSRFGRCSFFVIYDTESKSVEFIPNPNK
                     ENVEGAGPASVNLIASRDSKKVVSGEFGAKVKSLFDSLQIQLIVLNDEEKTINEIIEL
                     LNHNK"
     gene            1375..1572
                     /locus_tag="PKJ43_03250"
     CDS             1375..1572
                     /locus_tag="PKJ43_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 family protein"
                     /protein_id="HNZ71607.1"
                     /translation="MPQLNGTGPEMKGEKTGRGMGKCREVGTDEAREKLGTGMGLRRH
                     SGGGQGQGKRLKAGLDKIEKK"
     gene            1583..1810
                     /locus_tag="PKJ43_03255"
     CDS             1583..1810
                     /locus_tag="PKJ43_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HNZ71608.1"
                     /translation="MPGLNRKGPNGEGAMTGRRMGRCNPDNKGKTEDEILQNRTLSEP
                     SGLGMGQGLGRGLGRGRGLGRGKGMRFRGEA"
     gene            1909..2271
                     /locus_tag="PKJ43_03260"
     CDS             1909..2271
                     /locus_tag="PKJ43_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNZ71609.1"
                     /translation="MKQIIAIPMENGVLCAHFGHCQYFAIVEVDNGKIVSIKEETPPE
                     HVPGLYPRWVAQFCVTDVIGGGMGQKAIDLFNQQNINAFVGAPKKSAQELVTDFLEDK
                     LTLSANYCNHDENHSCGH"
     gene            2283..3137
                     /locus_tag="PKJ43_03265"
     CDS             2283..3137
                     /locus_tag="PKJ43_03265"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HNZ71610.1"
                     /translation="MISDYRCFFCFARAFEKLLEEEKLTVAEKNSFTRGMAMLYSKLQ
                     EDFSAPAFSRELHQVLKLYSKNTDPYLEIKKQSNDRVLALYPTLKKLVLESANPFDTA
                     LRLAIAGNIIDFGVSNQYDLDATIDKVLKSDFSINHSYELKEALSNAKTVLYLGDNSG
                     EIVLDKLFIETIMHPNLHFAVRGAPVINDVTIDDARYVGIYKVADVISNGYDAPSTII
                     DKCSEEFQKHFNDADVIISKGQGNLEGLLHKTHKTVFFLLMVKCDVIAEALGVKKGDF
                     VVMKNIQK"
     gene            3134..>3433
                     /locus_tag="PKJ43_03270"
     CDS             3134..>3433
                     /locus_tag="PKJ43_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNZ71611.1"
                     /translation="MIIAVASGKGGTGKTFVSTNLFWAAQKQGIPSTLVDCDAEEPND
                     AEFIPGYVLNTQNVVQKIPVIDDQKCTFCGRCFDYCTYNAIVYLPDKRFIKVVEEL"
BASE COUNT         1077 a          645 c          786 g          925 t
ORIGIN      
        1 tcacttccac atccttcatc cctttgtatt cgatcttttc tttgatggtg cgtaaccatg
       61 caatgtggct gtaaaaccca tcgaccgaca aaccaaccag cttacagttg tattcggcaa
      121 actggttttc cattgaagcg aaagtcatga attctgaagt gcaaaccggg gtaaaatcgg
      181 caggatgact gaacaagatc acccagcttc ctttaaaatc agaagggaaa ttgatggttc
      241 cctgagttgt aactgctttg aattctggtg cagtttcacc aatacgtggc attgaaatta
      301 cttcttgatt ttccatgtta attgtttttg ttgttattaa tttattgtcg ttgaatttgt
      361 tgtgctaact tctatactat tttataaaaa ggcatcataa aattacgaca aatatttgta
      421 aatgaccaaa tgatcattac ttttgttttg atcaaatgct cataattatg gctcggccaa
      481 aaagaatacg gaaagtaata aacccgcctc attttaaagg gtatcggcca cttggcctgc
      541 ccgaggaaaa caatccagtg atacttaact acgaagaata cgaatcgata aggttaagcg
      601 attttgagtt atatggacag catgaggctg ctcaggttat ggaagtttcg cgtccaacgt
      661 atgcccgcat ttacgaaagt gcacgacgaa aagtggccca ggcatttgtt tcaggtcgac
      721 ccattgtgtt cgaaggcgga aaagtgtatt tcgatagtga atggtattcc tgcacaggtt
      781 gtggctgttg gttcaatcat ccttcaaaag atcaggaaat caaaaactgc cctctttgcg
      841 gagctcccga tattaaacaa tattatgaag ataatgacca aagtgcaatt gaagatgtgt
      901 gtgtatgccc gaaatgtgga aaggaaaaga aacacttacc tgatactcca tgtcgaaaag
      961 aggtgtgtcc cgattgcaat agctttatga tcagaaaagg tgggccccat cattatcgta
     1021 atggaaataa aaactgttaa atacaatgaa agtagcaatt acatctaccg gcaatagcct
     1081 tgaatcgaag ttggacagcc gttttgggcg atgttcgttt tttgtcatct atgacaccga
     1141 aagcaaatcg gttgaattca ttccaaaccc gaacaaagaa aacgtggaag gtgctggccc
     1201 ggcatcggta aacctgattg cctcgcgcga ttccaaaaaa gtagtgtcgg gcgaatttgg
     1261 cgcgaaagta aaatcgctgt tcgacagcct tcagattcag ttgattgttt taaatgacga
     1321 agaaaaaacg atcaatgaaa tcattgaact tttaaatcat aataagtaaa cctaatgcca
     1381 caattaaacg ggacaggccc ggaaatgaaa ggagaaaaaa ccggaagggg catggggaaa
     1441 tgccgcgagg ttggaaccga tgaggcccgc gaaaaacttg gaacaggaat gggattgcgc
     1501 cgtcattcag gcggtgggca aggtcaggga aaacggctaa aagccggatt agataaaatt
     1561 gaaaaaaaat aaggaggtaa ttatgccagg attaaacaga aaaggcccga atggcgaagg
     1621 tgccatgacg ggtcgcagga tggggcggtg taaccctgat aataaaggga aaaccgaaga
     1681 tgaaatatta caaaacagga ctctttccga gccatcaggc ctgggaatgg ggcaaggctt
     1741 gggccggggt ttaggtagag gtcgtggact aggaagaggc aagggaatgc gtttcagggg
     1801 agaagcctga tggtgttcct aagggtgccg ggagtcgttc tttaaagtca ttcacaaatt
     1861 tacatcctgg catccttttt taatgattaa ttagctaaaa atcaagttat gaaacaaatt
     1921 atagccattc ccatggaaaa tggggtttta tgtgcccatt ttgggcattg tcaatacttt
     1981 gccatagtag aagttgacaa cggtaaaata gttagcatca aagaagaaac accacctgag
     2041 cacgtaccgg gtttataccc ccgttgggtt gctcagtttt gcgttaccga tgtgattggc
     2101 gggggaatgg ggcaaaaagc catcgacctg ttcaaccagc aaaacataaa tgcctttgtg
     2161 ggagcgccaa aaaaatcggc gcaagaattg gttactgatt ttttggagga caagctcacc
     2221 ctttcagcca attattgcaa ccacgacgaa aaccatagct gtggtcatta attcattgga
     2281 tgatgatttc agattatcgt tgtttttttt gctttgctcg cgcttttgaa aaattgctcg
     2341 aagaagaaaa gttgacagta gctgaaaaaa atagctttac ccgtggcatg gccatgttgt
     2401 acagtaaatt gcaagaagat ttttcggcac ctgctttttc aagggaatta caccaggtat
     2461 tgaagttgta ttctaaaaat accgatccat acttagaaat aaaaaagcaa agtaacgatc
     2521 gggttttagc tttgtatcca accttgaaaa agttggttct tgaatcagcg aatccttttg
     2581 atacagcgtt gcggttggct atagcgggaa acattatcga ctttggtgtt agtaatcagt
     2641 atgatttaga tgccacaatt gacaaggtgt taaaatctga tttttctatt aaccactctt
     2701 atgagttgaa agaagcactc tcaaacgcaa aaacagtatt gtaccttggc gataattcag
     2761 gcgaaatagt actcgataaa cttttcattg aaaccataat gcaccctaac cttcatttcg
     2821 cagttagggg tgcgcctgta attaacgatg ttacaatcga cgatgcccgt tatgtgggca
     2881 tctataaggt ggccgatgta atttccaacg gctacgatgc accatcgacc attatagaca
     2941 agtgttccga agaattccaa aagcatttta acgatgccga tgtgatcatc tcaaaaggcc
     3001 agggcaatct tgaaggtctt ttgcacaaaa cccacaagac tgttttcttc ctgttaatgg
     3061 taaaatgcga tgtaatagcc gaagcattgg gggttaaaaa aggtgatttt gttgtaatga
     3121 agaacattca aaaatgataa tagcagttgc aagcggcaaa gggggaacag gaaaaacatt
     3181 cgtttcgacc aacctgtttt gggctgccca aaaacaaggc atacccagca cattggtcga
     3241 ttgcgatgct gaagaaccca acgatgccga atttataccc ggttatgtgc tgaacacgca
     3301 aaacgtagtt caaaaaatcc cggtgatcga tgatcaaaaa tgcacatttt gcggcaggtg
     3361 cttcgattat tgcacctata atgccattgt ttatttgccc gacaaacgct ttataaaagt
     3421 tgttgaagag ctg
//
LOCUS       DAOPUW010000132         3428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_28122_length_3428_cov_3.499259, whole genome shotgun sequence.
ACCESSION   DAOPUW010000132 DAOPUW010000000
VERSION     DAOPUW010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28122_length_3428_cov_3.499259"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /gene="glmS"
                     /locus_tag="PKJ43_03275"
     CDS             complement(<1..1073)
                     /gene="glmS"
                     /locus_tag="PKJ43_03275"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNZ71612.1"
                     /translation="MCGIVGYIGGKNAYPLLMKGLKRLEYRGYDSSGIALIQNQKINV
                     YKKKGKVNELKVYCSDKDVSGNIGMAHTRWATHGEPNDINAHPHTSMKGLFTIIHNGI
                     IENYDKLHRDLALKGYTFYGQTDTEVLANLIEYMYVKGESVNAELAVRMALSRVIGAY
                     GLVVMCSEEPDRLIAARKSSPLAIGIGQGEFFIGSDATPMIEYTRNVVYLNDNDVAIL
                     SRNSFTLKTVDNDEEITPEIQQLELSVGEIEKNGYDHFMLKEIFEQPKTISDSFKGRI
                     NPVTKEVFLGGLSEVMPRILDARRIIIIACGTSWHAALIGEYLIEEYAQIPVEVEYAS
                     EYRYRKPIINKDDVVLAISQSGET"
     gene            complement(1138..1947)
                     /locus_tag="PKJ43_03280"
     CDS             complement(1138..1947)
                     /locus_tag="PKJ43_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNZ71613.1"
                     /translation="MEKKKVLFVFEEITPYLPESEKSIIGRYLPQGIQERGKEIRTFM
                     PRYGCVNERRNQLHEVIRLSGMNLVINDTDHPLIIKVASIQAARMQVYFIDNDDYFHR
                     KFVMTDDDGNPFDDNDERAIFFGRGVLETAIKLRWAPDLVHCHGWFSAIVPILIKRTF
                     ADDPLFQDAKVVISVYNDTFDKPFDEILPEKVQNDEITPDDVTILKEASYKSFYKLAI
                     QYCDGIVLGSDRIDKEIEECIAKSGKPVLSYQTEETYIEAYNDFYDKILNQ"
     gene            2099..2938
                     /gene="panC"
                     /locus_tag="PKJ43_03285"
     CDS             2099..2938
                     /gene="panC"
                     /locus_tag="PKJ43_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNZ71614.1"
                     /translation="MEVVRTIKDLSKIIDNQRVSGSKIGFVPTMGALHQGHISLVKRA
                     GEECNFVVVSIFVNPTQFNNKEDLERYPRDLGADVALLEKTSCNIVFAPTAEEMYPEP
                     DTRAFNFGDIENVMEGVYRPGHFNGVAQIVSKLFDAVRPDNAYFGLKDFQQLAIIKKM
                     VNMMNYPITIVPCAIVREADGLAMSSRNKLLSKEQRENATVISKTLFDAVKLKDNYDV
                     ESLKKWVTETINNNPYLDTEYFEIVNDTTLQPINNWDEKVNKIGCIAVHCGKVRLIDN
                     VMF"
     gene            2942..3292
                     /locus_tag="PKJ43_03290"
     CDS             2942..3292
                     /locus_tag="PKJ43_03290"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNZ71615.1"
                     /translation="MFIEVCKSKIHKVSVTEANLQYVGSITIDEALMDAANLIENEKV
                     QVVNLNNGERLETYVIKGKRGSGVICLNGPAARKVAVGDIVIIISYAMMDFEEAKSFK
                     PSLVFPDTETNMLI"
BASE COUNT         1047 a          693 c          643 g         1045 t
ORIGIN      
        1 gtttctccac tctggcttat ggccaaaacc acatcgtctt tgttaataat aggcttgcgg
       61 tatctgtatt ctgaggcata ttcaacttca acagggatct gcgcatactc ttcaatcaaa
      121 tactccccga tgagggcagc atgccatgat gttccgcaag caataataat gatgcggcgt
      181 gcatcaagaa tacgcggcat gacctcggaa agcccgccca agaacacttc tttggttact
      241 gggttgatac ggcctttaaa cgaatctgaa atagttttag gctgttcaaa tatttctttc
      301 agcataaagt ggtcataccc attcttttca atctcaccca cactgagttc gagttgctga
      361 atttcgggag taatttcttc gtcgttatcg acagttttca gggtaaaact attcctcgac
      421 aatatggcta catcgttatc gttcaggtaa acaacgttcc tcgtatattc aatcatcgga
      481 gttgcatcag aaccgatgaa aaattcgcct tgtccaattc ctatggctaa aggactactt
      541 ttccgggcgg caattagcct atcgggttct tcactacaca ttacaacaag cccataagca
      601 ccaattaccc tggataaagc cattcgaact gcaagttctg catttacaga ctcgcccttt
      661 acatacatgt attctatcaa gtttgccagc acctcagtgt cggtttgccc ataaaaagta
      721 tatcctttca acgctaagtc gcgatgcagt ttgtcatagt tttcaattat cccattgtga
      781 atgatagtga ataatccttt catggaggta tgggggtggg cattaatatc gttaggttca
      841 ccatgggttg cccaccgcgt atgagccatg ccaatgttcc ccgacacatc tttatcggaa
      901 cagtaaactt tcagttcgtt tacttttcct ttctttttgt aaacattaat tttctgattt
      961 tgaattaagg caattccaga agagtcgtaa ccacgatatt ctaatctttt caggcctttc
     1021 atgagtaatg ggtaggcatt tttgccacca atatatccaa caattccgca catactttat
     1081 tttattaggg ataaaaaaac aaaaaaaaga gaaagtaaaa ctttctctgt gatatgttta
     1141 ttgatttaaa attttatcgt aaaaatcgtt atacgcctca atgtaagtct cttctgtttg
     1201 ataactcagc acaggcttcc cgcttttggc aatacattct tcgatttcct tatctattcg
     1261 gtcacttcca agtacaattc catcgcaata ttgaatggct agcttgtaaa aacttttgta
     1321 agaagcttcc ttgaggatgg taacatcgtc gggtgtaatt tcatcatttt gcactttctc
     1381 cggaagtatc tcatcaaaag gtttatcaaa tgtgtcgtta taaacagaaa ttaccacttt
     1441 tgcgtcctga aaaagaggat catcggcaaa agtacgttta atcaaaatcg gaacaatggc
     1501 actgaaccat ccgtgacaat gaaccaaatc tggagcccaa cgcagtttaa tagctgtttc
     1561 gagcactccg cgcccaaaaa agattgcccg ttcatcgtta tcatcgaaag gatttccatc
     1621 gtcatcagtc ataacaaatt tacgatggaa ataatcatcg ttatcgataa aatacacctg
     1681 cattcgcgca gcctgaatcg aagccacttt aataattaaa ggatggtcgg tatcattgat
     1741 cacaaggttc attcccgaca aacgaatcac ttcgtgcaat tgattgcgcc gctcgttaac
     1801 acagccatat cggggcatga atgtacgaat ttcttttcct ctttcctgga ttccctgtgg
     1861 aaggtatcgg ccaataattg acttttcaga ttcgggtaag taaggagtga tttcctcaaa
     1921 gacaaacaat acctttttct tttccatttc taattaacca acaaattgat tttgcaaaag
     1981 tagtaaaatt acatgaataa tcaatccgta aatacaacag taacattagg caaaagattg
     2041 ggtgctatct tgttaaattc gctatttatt tatttacttt gcaattcatt tttgaaaaat
     2101 ggaagtagtt agaacgatta aagatcttag caaaatcatt gacaaccaaa gagttagtgg
     2161 ttcaaaaatt ggttttgtcc ctaccatggg agcgctacat caaggacata tttcgttggt
     2221 taagcgtgca ggagaagaat gtaattttgt tgtggtcagt atttttgtga atcccaccca
     2281 attcaataat aaagaagatt tagagcgtta cccacgcgat ttaggtgctg atgttgccct
     2341 gctggagaaa acaagctgca atatagtatt tgcaccaaca gctgaagaaa tgtatcctga
     2401 acctgacacc cgggcattca attttggtga tattgaaaat gttatggagg gtgtataccg
     2461 ccctggccat tttaatgggg ttgcacaaat agtaagcaag ctttttgatg ccgtacgtcc
     2521 cgacaatgcc tatttcggat tgaaagattt tcaacaactt gccattatta aaaaaatggt
     2581 aaatatgatg aactatccga tcactatcgt tccttgtgcg attgttcgcg aggccgacgg
     2641 gttggccatg agctcaagaa acaagttact tagcaaggaa cagcgtgaaa atgcaacagt
     2701 aatctcgaaa actcttttcg atgctgttaa actgaaggat aactacgatg ttgaaagcct
     2761 taaaaaatgg gtaactgaaa ccatcaacaa taatccctac ctcgataccg agtattttga
     2821 aattgttaac gacacaactc ttcaacctat aaataactgg gatgaaaaag tcaacaaaat
     2881 tggctgcata gctgttcatt gtggtaaagt gaggttaatt gacaacgtaa tgttttaaac
     2941 aatgtttatt gaagtttgca aatcgaaaat tcataaggtt tcggtaaccg aagctaacct
     3001 gcagtatgtg ggtagtataa ccattgacga agcgttaatg gacgcagcaa accttatcga
     3061 gaatgagaaa gtacaggtag taaacctcaa caacggtgaa cgcctcgaaa catacgtaat
     3121 taaagggaaa cgtggaagcg gcgttatttg cctcaacggc cccgctgccc gaaaagttgc
     3181 tgttggcgat atcgttatta ttatttctta tgccatgatg gattttgaag aagccaaatc
     3241 gtttaaaccc agtttggttt ttcctgatac agaaacaaat atgctgatat aatcaattta
     3301 tcgctatttt tttcacttca cgttttccat ctacattcaa taataagaaa tatacacctg
     3361 caggaagttg agactggttg gtgttaattt caaaagtatg ttttcctcca ttaagcattc
     3421 catcaaat
//
LOCUS       DAOPUW010000133         3420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_28194_length_3420_cov_3.647548, whole genome shotgun sequence.
ACCESSION   DAOPUW010000133 DAOPUW010000000
VERSION     DAOPUW010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28194_length_3420_cov_3.647548"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1061..2527)
                     /locus_tag="PKJ43_03295"
     CDS             complement(1061..2527)
                     /locus_tag="PKJ43_03295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNZ71616.1"
                     /translation="MTEETYEDAALILRRTKYYSGVFEREITEVNGVTNEREINFIFA
                     PDGLAAIYEKKNGTGNMYYVSTDNLGSINIIADAQGNIVNDLSYDAWGRRRDPYTWEQ
                     LTVAQTKALQPTLITDRGYTLHEHMDAFGLINMNGRAYDPLVGQFLSPDPFVQSATST
                     QSYNRYSYCWNNPLKFVDPSGYLGSLTWIDIQARNYAWGASNKNVDGIWFNTYWYEDQ
                     SQAFKSTLKLAIELQKQGNHLILQKNDDGYHIVYGVKFGQATTKSSYGSLSREEAVLM
                     CNLLNNGRQKDDNPYESHVMSQSWENNRDNPMRDVGMMHMGVYPGPSSWMRDFNRAFG
                     TTLNAGMAAGLAPIVAPALLEAAPRFALKYAGWKFLGSSLVQLTVNQRVDVFDATLDA
                     VTLPGLSRSTLGGFVDMRVSNNGFTFRTLGIDKTMGECLYDSGARYLFSSFGGYMNNQ
                     IGSTVKNGVDNAFYNFGTYYLAPTILNTVNKGVKNDIF"
     gene            3067..>3420
                     /locus_tag="PKJ43_03300"
     CDS             3067..>3420
                     /locus_tag="PKJ43_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71617.1"
                     /translation="MTRSIQNQRAKYFSRNAVVCFMKRTYHTNLAIAYNLGILPADIC
                     CAVPASTRHNWKTRGYNHLFGLDYSLEIQESTDVLRTIAQNKRLLRIARALVIIAGMY
                     RRLLLQVNDRKRIFLA"
BASE COUNT          947 a          702 c          722 g         1049 t
ORIGIN      
        1 aaaaacagca tgacaactcg caatcaaaaa agctcacgct ttgtgaagta ttttgcggag
       61 gtggggatgg tggagtagtt ggggaaggaa tgctgttgaa gaagatttgt ttatgttcag
      121 aaatggcact ctttagtccg aaatagtcaa acagaacaaa gcgctcgaaa taatttccga
      181 aatggtacat gactatactt atattcgccc gcatggatca ctcaacggtc tcactccaat
      241 ggaggcgctt atgaatgaaa acacggaatc cattgttcca accgatcaaa tcaaaatagc
      301 cgtaacgaaa agaatctctg aaaaccttaa ttcaaattgc atcgactgcc aaccgatacc
      361 ggaatagcag ttctttagac cgaaagaagt agaattttca aaaagaagca atgatttttt
      421 ctgctttctc tataaaatga acatttttct attcatttga taacccaata tgttcaaagc
      481 cggcgctgtg gccggctttg cgatataatg ttgaaaatct gtgttttacc aatattggga
      541 ttggtattta ggatttatcg tggatggtgc aagggtgttt gggatttatc ttggatggtg
      601 caagcctgca tgtattttca catggctaat ttatcatgat ttccgtgaac taaaaaatca
      661 ttttcatttc ggttgttgcc agtctggaat attcggaatt ttgtcccacc cttcgggcgg
      721 tgcttccctg atgctatctg gaactggaaa atccagtaaa agcttactat tttttggatt
      781 gtcaggattg aacattacgt aaaaatttct atttatcaga gatctgtaac taaaggaata
      841 atcagaacaa ggagagtctt gcatgtagcg aacgttattt acgaaataaa tataaaccaa
      901 gtcattgctt gtattagaat taacatcata tcttattgga gttcctattg taaatttgtg
      961 attgtcatgc aacatcctat tatttcttac aataataaaa gcaataatta gcaatattgc
     1021 tattaaagca actacaccta ttacttgaaa caatctgctt ttaaaaaatg tcattcttaa
     1081 ctcctttatt tacggtattt aatattgttg gagccaaata gtatgttccg aaattataaa
     1141 atgcattatc aaccccattt tttactgttg atccaatttg attgttcatg tatcctccaa
     1201 aagaggaaaa caggtatcgt gcaccggaat catacagaca ttcccccatt gttttatcaa
     1261 ttccgagtgt cctaaaagtg aagccattat tactaacgcg catatcaaca aatcctccta
     1321 aagttgatct gcttaatcct ggtaaagtaa ctgcatccaa tgtcgcatcg aaaacatcca
     1381 ctctttgatt gactgttagt tgaaccaatg aactacctaa aaatttccaa ccagcatatt
     1441 ttaaagcaaa cctgggggca gcttccagta aagcaggagc aacaattggc gctaatcccg
     1501 cagccatacc cgcattgagc gtggttccaa aagctctgtt gaaatcacgc atccatgaag
     1561 aggggcctgg ataaacgccc atgtgcatca taccaacatc acgcatcggg ttatcacgat
     1621 tgttctccca cgattggctc atcacgtggc tttcataagg attgtcatct ttctgtctgc
     1681 cattgttcag taaattgcac atcagaaccg cttcctcgcg gcttaagctt ccataactgg
     1741 atttcgttgt tgcttgtcca aacttcactc cgtatacaat atggtaccca tcatcattct
     1801 tttgtaaaat gagatggttc ccttgttttt gcagttctat ggcaagtttc agagtgcttt
     1861 taaatgcttg tgactgatct tcgtaccaat aggtgttaaa ccaaattcca tccacatttt
     1921 tattggaagc gccccatgca taattgcggg cttgaatatc gatccatgtt aaactgccca
     1981 gatacccact cggatccaca aacttcagcg ggttgttcca gcaatagctg tagcggttgt
     2041 agctctgagt gctggtggca gattgaacga atgggtcggg ggagaggaac tggcccacaa
     2101 gtggatcgta ggcgcggcca ttcatgttta tcagcccaaa ggcatccatg tgctcgtgca
     2161 gggtgtagcc tcggtcggtt atgagggttg gctgcaacgc ctttgtttgt gcaacggtga
     2221 gctgttccca tgtgtatgga tcgcggcggc ggccccaggc gtcgtagctc aggtcgttta
     2281 caatattgcc ctgtgcatcg gcaataatgt ttatgctgcc cagattgtcg gtcgaaacgt
     2341 aatacatgtt tccggttccg ttcttttttt cgtaaatggc ggccagcccg tcaggagcaa
     2401 agataaaatt tatttctctt tcgttggtca cgccattgac ttcggttatt tcgcgttcaa
     2461 acacgcccga gtaatatttt gtgcggcgca atatcagggc tgcatcttcg taggtttctt
     2521 cggtcatttt gcggcgccgt ggccggcttt gtgatataat gttgaaattc agtgtttacc
     2581 aatattgtca agggttgcca ggatttattg tggatggtgc aacagagctg aaataatttg
     2641 ccccaagtca ctcttcgaac aaattcgatg tttctgttca attgttttca aaaatcattt
     2701 gcacacactt aatcagttat tatcgaagat gataactgat ttgcattagc gttaatgcga
     2761 cctgctgagg gatttgaatt tcttcgcgtt cattcgtgtt tattcgcgga agacattcgg
     2821 cgggattagc tccacaaagt aacttcgctt cgatataagt gaaagagcga ttaaataaaa
     2881 aaacagagtg aaatcttcgc tctcgcgctg attctcactc tgtttttgtg aacctcacag
     2941 tgctgaattc gaactctttt ttagaggatt tgaagaagat cagcgattta tttttacttg
     3001 aataatccgc ctgcaatgca gtcgcaatgc gagggtgggg aattgcgtat atttataatt
     3061 tttctgatga cccgatcaat tcaaaatcaa cgggcaaaat acttttcgcg gaatgcagtt
     3121 gtttgcttca tgaaacgcac ctatcacacc aatctcgcaa tagcttacaa tcttggaata
     3181 ttgccggcgg acatttgttg cgctgttccg gcaagtacac ggcataattg gaaaacacgc
     3241 ggatacaatc atttgttcgg gcttgattat tcgttggaaa ttcaggagag caccgatgta
     3301 ttgcgaacga ttgcgcaaaa caagcgcctg ttgcggattg cgcgtgcgct ggtgatcata
     3361 gcaggcatgt atcgccgatt gcttctgcag gtcaatgatc gcaaacgaat ttttcttgcg
//
LOCUS       DAOPUW010000134         3416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_28229_length_3416_cov_2.993752, whole genome shotgun sequence.
ACCESSION   DAOPUW010000134 DAOPUW010000000
VERSION     DAOPUW010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28229_length_3416_cov_2.993752"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="PKJ43_03305"
     CDS             complement(<1..1156)
                     /locus_tag="PKJ43_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HNZ71618.1"
                     /translation="MSTNILNMKPLVISLLLLLVSTFVNAQANTCGDPVWSDEFNYEG
                     APDPSKWGYDVGGGGWGNQELQTYTNNRNNSWVADGKLFIKAIKTNGNWSSARLVTRQ
                     KGDWTYGRIEIRAKIPAGRGIWSAIWMLPTDFSYGNWPNSGEIDIMEHVGYDVNRIYG
                     TIHTGAFNHTLGTQKGSNTMEPTAYTEFHDYAVEWDAENISIFVDEKRYFTFQNLHKT
                     SAEWPFDKRFHLLLNIAIGGSWGGAQGLDGTITEAVMEIEHVKVYNLAPQQPVIIGPE
                     FVQPNEEVSFECQQVSNASFLWDFPEDVQIIDGQNTNLINVIWGETSGPVKVKLQTPC
                     DTLDANPLNVKLTTELTPLIVQNFSGENTTWKVNETATNTVTLEKEGSENL"
     gene            complement(1146..1997)
                     /locus_tag="PKJ43_03310"
     CDS             complement(1146..1997)
                     /locus_tag="PKJ43_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNZ71619.1"
                     /translation="MKRGIKIGSLASLMLIVTFLFAACGNKQKKTASRLDNFELIWAD
                     EFEYNGLPDSAKWNYDTVGNAWGWGNHELQYYTVARSQNTWVSDGTLKITANKENLNG
                     FNYTSGRLTTRQKGDWLYGRFEIKAKLPDGRGLWPAIWMLPTDWEYGGWPKSGEIDIM
                     ENVGFDPYTIVASVHTGNYNHAIKTERNNKMTLKTNRSQFHTYALEWTEKQIDAFVDD
                     SLYFTYQKESDDPMVWPFNKNFHLLLNVAVGGDWGGKQGVNDSIFPVAMEVDYVRVYK
                     KRLHNEH"
     gene            complement(2208..>3416)
                     /locus_tag="PKJ43_03315"
     CDS             complement(2208..>3416)
                     /locus_tag="PKJ43_03315"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HNZ71620.1"
                     /translation="NTNNEGGVLRLQEDGTFSNVSYPFYKLKGKFIGSFFHFNVIDEE
                     NVLIALEKGFAHYNPQMVIDYRRGYNSAINRVSLLGENTLLYSGLNIGKNAAGQKNVP
                     EIKYRNNSLRFTFSAQFFEGKKETQYSYQLVGFDKNWSDWQPESVKEYTNIPDGSFVF
                     NVRTKNKYGVISEAGSFEFIIQPPWYKSLFAIILYLIIASIIIWLLILLFLKKVEKSR
                     IKEKEIQQKEFREREEKLKNETLLAEKEIIRLKNEKLNAEMIHKDKELANTAINLVQK
                     NKQLNKIKIDLQKIQSELKEELVKNRIAMIIRKIDRETSNDESWSIFETNFEQVHEDF
                     LKRIREKHPDITPKELKLAAYLRMNMSSKEIAALMNITTRGVEISRYRLRRKLDIERT
                     QNLIDYILSV"
BASE COUNT          876 a          721 c          668 g         1151 t
ORIGIN      
        1 gaaggttttc actgccttct ttctcaagcg tcacggtatt tgttgccgtt tcatttactt
       61 tccatgttgt gttttcgccc gagaaatttt gaacgataag tggcgttaac tcagttgtaa
      121 gctttacgtt cagtgggttt gcatcaagtg tgtcgcatgg ggtttgtagt tttaccttta
      181 ctggccccga tgtttctccc catataacat ttatcaagtt tgtattttgc ccgtcaatta
      241 tttgtacatc ttcaggaaaa tcccagagga aagatgcatt cgatacttgt tggcattcaa
      301 aacttacctc ttcgttgggc tgtacaaatt cgggtccgat gattacgggt tgttgcgggg
      361 caaggttgta taccttcaca tgttcaattt ccatgacggc ttcggtaatt gtaccgtcta
      421 aaccttgagc acctccccaa gaacccccga tggcaatatt gagcaacaaa tggaagcgtt
      481 tgtcgaatgg ccattcagcg cttgttttgt gcaagttctg aaaagtaaag tagcgctttt
      541 catcaacaaa aatcgagata ttttctgcgt cccattccac cgcgtaatca tgaaattcgg
      601 tgtatgccgt tggctccata gtattgcttc ccttttgtgt gccaagggtg tgattgaatg
      661 ctccggtatg tatggtccca tagatgcggt ttacatcata gccaacatgc tccataatat
      721 ctatttcgcc actattgggc cagttgccgt atgaaaagtc tgttggaagc atccaaattg
      781 cagaccagat tcctcgtcct gcgggaattt tcgcccttat ttcgattctg ccatatgtcc
      841 aatcgccttt ttgccgggta accaatcggg ccgaggacca gtttccattg gtttttatgg
      901 ctttaatgaa aagtttccca tcggccaccc aagagttatt gcggttattt gtataggttt
      961 gtagttcttg gtttccccag ccgccaccgc caacatcgta tccccatttc gaagggtcag
     1021 gggcaccttc gtagttgaat tcgtcgctcc aaaccggatc gccgcaggta ttggcctggg
     1081 cattaacaaa ggtgctaact aacagtagca gaagcgagat aaccaacggt ttcatattca
     1141 atatattagt gctcattatg tagtcttttt ttgtacaccc tgacataatc aacttccatg
     1201 gccaccggga aaattgaatc gttaacgcct tgttttccgc cccaatcgcc acccacagca
     1261 acatttagca acaggtgaaa atttttgttg aaaggccaca ccattgggtc gtcgctttct
     1321 ttttggtatg taaaatagag tgagtcgtcc acaaaagcat caatttgttt ttcggtccat
     1381 tctaacgcat aagtatgaaa ttgagaacga ttggttttta gtgtcatttt gttatttctt
     1441 tccgttttga tggcgtggtt atagtttccg gtatgtaccg aggcaacgat agtatatggg
     1501 tcgaaaccaa cattttccat aatgtcaatt tcgccgcttt tgggccaacc gccatattcc
     1561 caatcggtgg gaagcatcca gatggctggc caaagccctc gtccatcggg aagttttgct
     1621 tttatttcga accgcccata aagccagtcg cctttttggc gggtagttaa cctgcctgag
     1681 gtataattga agccatttag attttctttg ttcgccgtaa tcttaagggt tccgtcagat
     1741 acccatgtgt tttggcttcg tgctacggta taatactgaa gttcgtgatt tccccaaccc
     1801 caggcgtttc ctacggtgtc gtaattccac tttgcgctgt cgggaagccc gttatattca
     1861 aactcatcag cccaaatcag ttcgaagttg tccaggcgcg atgcggtttt cttttgcttg
     1921 ttaccgcagg ctgcaaaaag aaaggtcaca attagcatta agctagccaa agacccaatt
     1981 tttatccccc ttttcatttt aatcagtttt aatgaagcac tcgtaaaaac tttcatgttg
     2041 aaaataacta aggcgcatta ttgttcgccc aaatcaatta cgaagtaata aaatactgaa
     2101 ttacaagtca aaaaaggtag attaacaaaa acacatcggg gagtttgcac aatttcatca
     2161 atagtacatc agtttaatca taatttatta aaaatcaaat taataattta aaccgacaag
     2221 atataatcaa ttaagttttg agtccgttca atgtctaatt ttctgcgtaa tcggtagcgg
     2281 ctaatttcca caccacgggt agtaatattc attagggcgg caatttcttt gctcgacatg
     2341 ttcatgcgta aataggcggc aagttttaat tcttttgggg taatatcagg atgtttttcc
     2401 ctgattcgct tcaggaaatc ttcgtgaacc tgttcaaaat tcgtttcgaa aattgaccag
     2461 gattcatcgt tgcttgtctc ccgatctatt ttcctgataa tcatggcaat tctgtttttt
     2521 acaagttctt ctttcaactc ggattgaatt ttttgcagat caatttttat tttattcagc
     2581 tgtttgtttt tttgcacaag gtttattgcc gtgttggcca attccttgtc tttatgaatc
     2641 atctcggcat tgagtttttc atttttcagt ctgataatct ctttttctgc cagtaatgtt
     2701 tcatttttca gtttttcttc gcgctcgcga aattcttttt gctgtatttc cttttctttt
     2761 attctcgatt tttcaacttt ttttaggaaa agcaatataa gcaaccaaat aattatgctg
     2821 gctataatga gatataatat aatggcaaat aatgatttat accatggtgg ctgaataatg
     2881 aattcaaatg aaccagcctc cgatatcacc ccatatttat ttttggtacg aacgttaaaa
     2941 acgaaacttc cgtcggggat gttggtatat tctttaaccg attctggctg ccaatcggac
     3001 caatttttat cgaagccgac caactgatag gaatattgcg tttctttttt cccttcgaaa
     3061 aattgtgctg aaaaagtaaa gcgtagcgag ttattcctgt attttatttc cggtacattt
     3121 ttttgaccgg ctgcattttt cccaatattc aacccggaat aaagcaaggt gttttcccct
     3181 aataacgaaa cccggttaat cgccgagtta tagcctctgc ggtaatctat taccatttga
     3241 gggttgtaat gggcaaagcc tttttcgagg gcaatgagca cattttcttc atcgatgaca
     3301 ttaaaatgga aaaaactacc gataaacttt cctttcaatt tataaaatgg gtacgagaca
     3361 tttgaaaatg ttccatcttc ctgaagcctg agaacaccgc cttcgttgtt ggtatt
//
LOCUS       DAOPUW010000135         3391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_28507_length_3391_cov_2.514089, whole genome shotgun sequence.
ACCESSION   DAOPUW010000135 DAOPUW010000000
VERSION     DAOPUW010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28507_length_3391_cov_2.514089"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..653
                     /locus_tag="PKJ43_03320"
     CDS             72..653
                     /locus_tag="PKJ43_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71621.1"
                     /translation="MNELKKGLLPLVLIVISGAFLAVSLIVLLRGNKSGKWISRKMKL
                     GGLILSLTALINACDPAQTAALTDGNPPWTCYIIAMPDSTEQKKQNIFYVETNNDSLI
                     TLNLRRESQIKGEIRNRTYETFSFAVVDQQGNKLQRDNILASDGVFDERIEPFSIDVD
                     KKLPNGLYLIRFFESDKENQPEWSEQYFNLRLN"
     gene            700..1230
                     /locus_tag="PKJ43_03325"
     CDS             700..1230
                     /locus_tag="PKJ43_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71622.1"
                     /translation="MDTKRVLSPIILVGLFAAFALVCLLVWLLKGRAGKLITGKMKLG
                     AAIIAITGVSTGCPPVITCYDPMPQDSFQFDSIDYNDYSVFANLPNDSVLTGTVIEPT
                     YNQYQFKVSTTDSQLVDHGMVQAVDGTFDNSSEAFKIILNPQIDAGTYQLSILPYQRT
                     TDIPEYEVQNIRLKIK"
     gene            1239..2309
                     /locus_tag="PKJ43_03330"
     CDS             1239..2309
                     /locus_tag="PKJ43_03330"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ71623.1"
                     /translation="MIWERRKKAELSYILYEATERCNLDCIFCYNHWKRDGYTHNPPA
                     EDYTNSLKTLKKLFSIASVKHVTFTGGEPMLAERIGELILFCRMKGASVSLITNGNHA
                     SFEELSQLINLGVQLFELPVHAADAQTHDRMTNVQGSWEKSRATIGYLNRMGAYVVPV
                     MVITKFNYNQVAETLEMIHSMGLNRVMINRYNIGGKGVKTPEEVSATQNQLNEAFAQA
                     NEKARTLKMTVSSNVCTPHCVVNPRHYPSIQFTNCSPDITHRPLTLNTSGDLRFCNHS
                     PSVLGNIFTNKVDEIIANGQKNACMDQRPDYCSACNLYEKCLGGCRAASEQSGNTFSE
                     VDPIVFLTSKKNTLKDTYHEPV"
     gene            2296..3138
                     /locus_tag="PKJ43_03335"
     CDS             2296..3138
                     /locus_tag="PKJ43_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71624.1"
                     /translation="MNLYNKINLLLIVLISAGCSKNDFDANSPSGLVAEITNGEGEIV
                     SRYTYNKKQQVTESWHTDIAFSSNERQHYKYIYNNSNKLTEKKEYEPGIIYMSSITGA
                     SGKDVDYQYSYDDKGRLKSIKVDYDYDIEGWVNADYSRLSTFEYPDDKHVIVTVSTID
                     PAGNSIPGFSKYYYNEKGNIEKTTDNVIIDNKERIISQAVFTYDTMKAPRYPDPGPVS
                     KSNVLTRTETSYNYNEAEIMSVAYTSVFQYEYTYNDYGYPLTQVETYPNEIKNSKQFK
                     YVKQ"
     gene            3135..>3391
                     /locus_tag="PKJ43_03340"
     CDS             3135..>3391
                     /locus_tag="PKJ43_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNZ71625.1"
                     /translation="MKTKLFTAVIALFILFACEKADDKPGNTDLSEKSEQLIKADNEF
                     GLEMFSRIYNSPECPENYMISPLSISLALSMAYNGANTQTK"
BASE COUNT         1151 a          757 c          659 g          824 t
ORIGIN      
        1 aagatacggg cgcgatcctt tttgttggga aaatggttga cccaacatta aacaattaaa
       61 aacaacagat aatgaacgag ctaaaaaaag gactcctgcc gcttgtactg atcgtgatat
      121 ccggggcgtt tctagcggta agcctaatag tgctgctgcg tggcaataaa tcggggaaat
      181 ggatcagccg gaaaatgaag cttggaggcc taattttatc gcttactgcg ttaatcaatg
      241 cttgtgaccc cgcgcaaaca gcggcattga ccgatggcaa cccaccatgg acatgttaca
      301 taatagccat gcctgattca accgaacaaa aaaagcagaa tatattctat gttgagacca
      361 ataacgatag cctgatcacg ttaaacctcc gtcgggaaag ccagataaag ggagaaatac
      421 ggaaccgcac ctacgaaaca ttttcgtttg ccgtagtcga ccagcaaggc aacaagttgc
      481 aacgcgacaa catcctggca tccgacgggg tttttgatga acgcattgag ccgttcagta
      541 tagatgtgga caagaaacta cctaatgggc tttacctcat ccgatttttc gaaagtgaca
      601 aagaaaatca gcctgaatgg agcgaacaat actttaatct acggttaaac taaataaaga
      661 aaccgtaact tgcttattat aatagataag ggaaaagaaa tggatacaaa aagagtttta
      721 tcaccgatca ttcttgttgg actatttgcc gcctttgcac ttgtttgcct cctggtttgg
      781 ctgctaaaag ggcgtgcagg caaacttatt acaggcaaaa tgaaacttgg cgcagcaata
      841 attgccatca caggagtttc taccggatgc ccgccggtta ttacctgtta cgacccaatg
      901 ccccaagact cgtttcagtt cgacagtatc gattacaacg actattcggt ttttgccaac
      961 ctgccaaacg attcggtact taccggcaca gtaatcgaac caacgtataa ccaatatcag
     1021 tttaaagtaa gcacaaccga cagccagctg gttgatcatg gaatggttca agctgttgat
     1081 ggcacattcg acaatagcag tgaagcattt aaaataatcc ttaacccaca gattgatgct
     1141 ggaacttacc aactttcaat tcttccgtat cagcgaacaa ctgacatacc cgaatatgaa
     1201 gtacagaaca tcaggttgaa aatcaaataa ttcgcagcat gatttgggaa agacgaaaaa
     1261 aagcagaatt aagctacata ttgtacgaag caaccgagcg ctgtaacctt gattgcatct
     1321 tttgttataa tcactggaaa agagatggat acacacacaa tccacctgcc gaagattaca
     1381 cgaattcgct caaaacatta aaaaaactat tttcgatagc ctctgtaaaa catgtaacct
     1441 ttacgggcgg tgaacctatg cttgccgaaa gaattggtga actcatcttg ttttgccgca
     1501 tgaaaggcgc ttcggtttca cttatcacca atggcaacca tgcctcattc gaagaacttt
     1561 cacaactcat aaaccttggt gttcagcttt ttgaacttcc agtccatgct gccgatgccc
     1621 aaactcacga tcgaatgacc aacgttcagg gatcatggga aaaatcacgg gctacaattg
     1681 gatatctcaa ccgaatggga gcgtatgtgg ttccggttat ggtaattaca aagttcaact
     1741 acaatcaagt tgctgaaacc ctcgaaatga ttcattcgat gggcttaaac agagtcatga
     1801 tcaatcgcta taacattgga gggaagggtg ttaaaactcc tgaagaagta tcggctacac
     1861 aaaatcaact gaatgaagct tttgcccagg caaacgaaaa agcccgtaca ctaaaaatga
     1921 cagtatcatc gaatgtttgc accccacact gcgtggttaa tcccagacat tatccatcga
     1981 tccagtttac caactgctcg cccgacatca cacacagacc gttgacatta aacacatcag
     2041 gcgatcttcg tttttgcaac cactctccct ctgtgctggg taacattttc accaataaag
     2101 ttgatgaaat aattgccaac ggccaaaaaa atgcttgcat ggaccaacga ccagactatt
     2161 gctctgcttg caatctttac gaaaaatgtc ttggcggatg ccgtgctgct tcagaacagt
     2221 cgggaaatac cttcagcgaa gtcgacccga ttgtttttct tacatcgaaa aaaaatacat
     2281 taaaagacac ctatcatgaa cctgtataat aaaataaacc ttctgctcat cgtgcttatt
     2341 tctgccggat gtagcaaaaa tgacttcgat gcgaacagcc cgagtggatt ggttgctgaa
     2401 attaccaacg gagaaggaga aattgtttcc cgctacactt acaataaaaa acaacaagtg
     2461 accgaaagct ggcacaccga tatcgcgttt tccagtaatg agagacaaca ctacaagtat
     2521 atatacaaca attccaacaa attaaccgaa aaaaaagagt acgagcctgg cataatatat
     2581 atgtcgagca tcactggggc aagcgggaaa gatgttgatt accagtattc ttacgacgat
     2641 aaagggagat taaaatcaat taaagttgat tatgattacg acatcgaagg atgggtaaat
     2701 gccgattata gccggctaag cacatttgaa tatccagatg acaagcacgt aatcgtgaca
     2761 gtcagcacca ttgatcctgc cgggaattca attccaggat tcagcaaata ttattacaat
     2821 gaaaagggga acatagaaaa aaccactgat aatgtaataa tagacaataa ggaacgaatt
     2881 atatcacaag ccgttttcac ctacgacacg atgaaagctc cccgttaccc cgaccctggc
     2941 ccagtttcga aaagtaatgt tctcacgcga actgaaacct cctataacta caacgaagca
     3001 gaaataatga gcgtagccta cacttcagtt tttcaatatg aatacacata caatgactat
     3061 ggataccctt taacacaagt tgaaacatat ccgaatgaaa ttaaaaacag caaacaattc
     3121 aaatacgtaa aacaatgaaa actaaactat tcacagcagt aattgcccta ttcatactct
     3181 ttgcctgcga aaaagcagac gataaaccgg gcaacaccga tttaagcgaa aaatcggaac
     3241 aactgatcaa agccgataat gaatttggcc tcgaaatgtt ctcacggata tacaactcac
     3301 cagaatgtcc agaaaactat atgatttcac cactaagtat ttctctggcg ctatcgatgg
     3361 cttacaatgg cgccaacacc caaaccaaag a
//
LOCUS       DAOPUW010000136         3385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_28574_length_3385_cov_2.845045, whole genome shotgun sequence.
ACCESSION   DAOPUW010000136 DAOPUW010000000
VERSION     DAOPUW010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28574_length_3385_cov_2.845045"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..979
                     /locus_tag="PKJ43_03345"
     CDS             296..979
                     /locus_tag="PKJ43_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNZ71626.1"
                     /translation="MKKLVVLTGAGISQESGIKTFRDSDGLWEEYNIEDVATPEAWKK
                     NPELVMRFYNERRKQLLDCKPNAGHFGLAKLEVLFDVSIITQNIDDLHERAGSTKVLH
                     LHGELKKSRSTVDENLINDIDGWELKMGQKCAKGSQLRPHIVWFGESVPAISDAIPMV
                     KAADIFVVIGTSMNVYPAAGLIHYVKPNTPVFLIDPKPVDVYQKVIFIQEKAGKGLEI
                     LTKELQKLL"
     gene            1120..1848
                     /locus_tag="PKJ43_03350"
     CDS             1120..1848
                     /locus_tag="PKJ43_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNZ71627.1"
                     /translation="MSSTDKGYIIQTSGSVNNKFSSRVSASIKGRTTFSRILLFVIFI
                     VLAKPGLSQGFDGGLLAGFNGSQVRGDLYSNGFHKMGLLAGAWVQTSLNDNFYWTMEL
                     KYSQKGSRMNPSKKNDYYLYIYRLNYIDIPVAFGYHNSDYYSFLMGLSFNYLMSRSAE
                     DSFGEIELSQNLHSWEIAAFAGIRIDFEQMVRRDWASKFKLDFRFQFSALPIYTSNNK
                     LFYYSPYSQFNSVISTALYYTVDW"
     gene            1870..>3385
                     /locus_tag="PKJ43_03355"
     CDS             1870..>3385
                     /locus_tag="PKJ43_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNZ71628.1"
                     /translation="MGKKDKSSFKKKKGINKKELKQMVLELFYKDSEKILTPRQICSM
                     LAVSDLSTKQLVNVAIEELSDDGYLEQKERGKFQLIAKTGYVVGIVDMTQKGYAFIVS
                     DELEEDIFVAGPNLNRALNGDKVRVYLNAKRKRKQPEGEVVEIIERAKTNFVGVVSMS
                     KNFAFLVPSGKNNFDIFIPKDKLKGAKEGQKAVAKITDWPARAKNPFGEIVDVLGEPG
                     HNDTEMHAILAEFDLPYKFPERVNSAAEKLPVEITAEEISKRRDFRNITTFTIDPGDA
                     KDYDDALSIQKLENGYWEVGVHIADVSHYVQPGSLIDNEAYERATSVYLVDRVVPMLP
                     ERLSNGVCSLNPNEDKLCFSAVFEIDDDATVRKHWIGRTVIHSNRKFSYEEAQQIIET
                     GEGELKDEMLTLHRLAQKLRSGRYQNGAISFDRAEVKFKLDESGKPLGVYFKEAKESN
                     QLIEEFMLLANKKVAETIGKVKDKSKARTFVYRIHDPPDPDKLESFNNFIGKFGY"
BASE COUNT         1063 a          624 c          764 g          934 t
ORIGIN      
        1 aaggctacgg acttaaagtg gttgaaaata ttcccattga agtagaacca aacgaatata
       61 acgagtttta tatgaagaca aaacgtgacc ggatggggca tatcctccag aaattcaagt
      121 acgaaagtga agaataacag gggtcaagcg tttaattttc agtgatattg atcttgcgaa
      181 atttttttaa tagtccgaaa tttaattatt taaagcaagc tatccagcat taatctcagt
      241 aaaagaacag aaagtagatc taaaagatac ctagtaagtt aaaagttttg ttgagatgaa
      301 aaaattggtt gttttgacag gtgcaggcat aagtcaggaa agtgggatta aaacctttcg
      361 cgattccgat ggcctttggg aagagtataa cattgaagat gttgccaccc ctgaagcctg
      421 gaaaaagaat cctgagctgg tgatgcgttt ttataacgaa cgacgaaaac agttgcttga
      481 ctgtaaacct aatgctggcc atttcgggtt ggcaaaactt gaagtacttt tcgacgtaag
      541 tataatcaca caaaatatcg atgatttgca cgaaagggcc gggagtacaa aggtgcttca
      601 tttgcatgga gaactgaaaa aatcccgcag tacggtcgat gaaaatctga ttaatgatat
      661 tgacggctgg gaactgaaga tgggacaaaa gtgtgccaaa gggagccagc taagacctca
      721 catcgtgtgg ttcggagaat ctgtccctgc tatctccgat gcaattccaa tggttaaagc
      781 cgccgatatt ttcgtggtaa ttggcacttc aatgaatgtt tacccagcgg caggcctaat
      841 tcattacgtt aagcccaaca cccctgtttt ccttatcgat cccaaaccgg tcgatgttta
      901 ccagaaagtt attttcattc aggaaaaagc aggaaagggg cttgaaatat taaccaaaga
      961 actccaaaaa ttattgtaaa ttcataacct gaaactgatt ttcagtttta caaggttttg
     1021 tacttcattg cttgtttcgt atttgagcag ttattattat gttgagaagc aagctttttt
     1081 gaatacacct tgttgattgt tagttgttaa tagttttata tgagcagtac agacaagggg
     1141 tatataatcc aaacttctgg cagtgtaaac aataagtttt cttcacgtgt ttcggcatcg
     1201 ataaaaggaa gaaccacatt cagtcgcatt ttattattcg taatatttat tgttttagca
     1261 aaaccagggc tatctcaagg tttcgatgga ggcttgctgg cggggtttaa cgggagtcag
     1321 gttagaggtg atttatattc taacgggttc cacaaaatgg ggttgctggc tggtgcctgg
     1381 gtacaaacat cactgaacga caatttctat tggacaatgg aattgaaata tagccagaaa
     1441 ggcagccgga tgaacccgtc gaaaaaaaat gattattacc tatatatcta ccgccttaat
     1501 tatattgata ttccggtggc tttcggatat cacaacagtg attattattc gtttcttatg
     1561 gggctctctt ttaattattt aatgagccga tcggccgaag atagctttgg ggaaattgag
     1621 ttaagccaaa accttcatag ttgggaaatt gctgccttcg caggaatacg catcgacttt
     1681 gagcaaatgg taaggcgcga ttgggcttca aaattcaagc tcgatttccg gttccaattt
     1741 tcggcattac ctatttatac aagcaataac aaactttttt attattcacc atacagccag
     1801 tttaacagcg taatttctac agcattatat tatactgtcg actggtagta caatattaac
     1861 tatccgatta tgggaaaaaa agacaaaagc tctttcaaaa aaaagaaagg cataaataaa
     1921 aaagagttga agcaaatggt gcttgaactt ttctacaaag attcggagaa aattcttact
     1981 ccgcgacaaa tatgttcgat gcttgctgtt agcgatcttt caaccaaaca gcttgtcaat
     2041 gtagctattg aagaattgtc agacgacggc taccttgagc aaaaagagcg cggcaagttt
     2101 caactcattg ccaaaaccgg ctacgtggtg ggcattgtcg atatgactca gaaaggctac
     2161 gcctttattg tatccgacga attggaggaa gatatttttg tagccgggcc aaatttgaac
     2221 cgtgcgctga atggcgacaa agtaagggtt taccttaatg ccaagaggaa acgcaaacag
     2281 ccagaaggag aagtggttga aataattgag cgtgcaaaaa ccaattttgt cggggtggtt
     2341 tccatgtcga aaaattttgc atttcttgtg ccgtcgggga aaaacaactt cgatattttc
     2401 attccaaaag ataagttaaa aggggcaaaa gaagggcaaa aagcggtagc taaaattacc
     2461 gactggccgg ctcgagctaa aaatcctttt ggcgaaattg ttgatgtact gggtgaacca
     2521 gggcacaacg acaccgaaat gcatgccatc ctggccgagt tcgacttgcc ctataaattt
     2581 cctgagaggg tgaacagtgc tgctgaaaaa cttccggtgg aaattaccgc tgaagaaata
     2641 tcgaaacgtc gtgatttcag gaatataacc acttttacta tcgacccggg tgatgcgaaa
     2701 gactatgatg acgctctttc gatacaaaaa ctggaaaatg ggtactggga ggttggtgtt
     2761 catattgccg atgtaagcca ctatgttcag cctggttcgc tcatcgacaa tgaagcatat
     2821 gaacgtgcta catctgtata tcttgttgac agggtggtgc ctatgctccc tgaaagactt
     2881 tcgaatggcg tgtgttccct caacccgaac gaagataagc tttgcttttc tgctgttttc
     2941 gagatcgacg acgatgccac agttcgaaaa cactggattg ggcgcactgt gattcattca
     3001 aaccggaaat ttagttacga agaagcccag caaattatcg aaacaggcga aggtgagtta
     3061 aaagacgaaa tgcttaccct gcatcggctt gcccaaaagc ttcggtcggg aaggtatcaa
     3121 aacggggcta tttcgttcga tagggccgag gtgaaattca agctggatga aagcggaaaa
     3181 cctcttggag tgtattttaa agaagccaaa gaatcgaacc agcttatcga ggaatttatg
     3241 cttctggcca ataaaaaagt ggctgaaact attggtaaag tgaaagataa atcgaaagcc
     3301 cgcactttcg tttaccgtat ccacgatccg ccagatcccg acaaacttga gtcgttcaat
     3361 aatttcatcg gaaaattcgg gtatg
//
LOCUS       DAOPUW010000137         3379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_28648_length_3379_cov_3.925692, whole genome shotgun sequence.
ACCESSION   DAOPUW010000137 DAOPUW010000000
VERSION     DAOPUW010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28648_length_3379_cov_3.925692"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PKJ43_03360"
     CDS             complement(<1..541)
                     /locus_tag="PKJ43_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HNZ71629.1"
                     /translation="MKTIEIFCENNNQYKEYPLGTTLLEIAGDMGIEMPFPICGAMVN
                     HKVRELTFEVVKPKDIRFIDYSHPDGKRIFTRSLFFILYVAVRDIMPKAKLKIDHGIS
                     RGYYCEFENLPAPLSDDFVEKIKQRMLEIIADDVPFERRGKQMERILNEYEAAGLSDK
                     VELFEGTGWLYAYVYYLNNN"
     gene            complement(705..>3379)
                     /locus_tag="PKJ43_03365"
     CDS             complement(705..>3379)
                     /locus_tag="PKJ43_03365"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNZ71630.1"
                     /translation="WYCIKAVFSNGESPISNIISVTPGYHPIIVNAPTYNSVLSLNGE
                     IDAYKFKTTTSGLYTIETHGELDTYLTLFDYDATTQLHFNNDGGTNRNAKITYSLFAD
                     TWYYFEVKGVNSSVVGNYSVDVKGGEPLVLYPPSNVTATTANGKMAINWMTPDAGAPI
                     GYNVYLGNSESGAYTLAVENTTDLSVTLSGLSNGIKYWAYVSAIYEIGESTASNKVYG
                     IPSAGTPPPAPIALAPLSVGMDFFTANWGNTEGSTGYYIDISDNIDFTSFLTGFQHKN
                     VGDVTSFSITGLTANTSYYYRVVAYNEAGSSNYSNAVMVHTSAATVPSVPVAIAGTDV
                     SAEGFTANWNWAENATGYFLDVSLNSSFSSFLPGFENKNVGNVCSFTLDGLETNTEYF
                     YRIKAINLNGSSSYSNIISVRTLSSINCFNIISPKPGATAVSLNPVISIEFCVDQIIV
                     KSDGYIILSDQSMEQILGVNYFVFSLSDMLVKNNRIDIQAPSLKENMTYTVSIPYGVI
                     TDLAGNPFSGLPTPKYWMFSTGNSSSEIVSVQSVTIPNNASGTVTVTCSKAAKVYLVR
                     NDVPKDVISIKNAIQQNKCREANLLSPGSVVMNAENLLPGTYYVCAIETSGAMSFAVN
                     EVIVVSSNELSLKSIKQIQGEVSSSPISGTLVKVRGTIAAITTDGFFMQDDNAPWCGI
                     FVRSTIPVSQNLSVEIVGTVAEINGMTTIDNVSEIKTILQVVQLQPISITSSLAEKHE
                     GVLCYITGKSSETKNTSGTWQAGNYLVDNSLVGSFSLEEGNNYGVTGIVSEDNNKYKV
                     LSTQIVNLTTGTDGFNIFDNVTLYPNPFDEELYIMNAHCVESIVIFNQSGQLVRELTN
                     PGQTINTSNLRKGLYVVLIQTTEGTHRIEKVFKQ"
BASE COUNT         1019 a          719 c          640 g         1001 t
ORIGIN      
        1 tgttgttatt caggtaatac acataagcgt agagccaacc tgtgccttca aaaagctcga
       61 ctttgtctga aagccctgcc gcttcatatt cattcagaat gcgttccatc tgcttgccgc
      121 gccgctcgaa tggtacatcg tcggcaataa tttcaagcat gcgctgctta attttttcga
      181 caaaatcatc gctcagaggg gcaggaaggt tttcgaactc gcaataatac ccacgcgaaa
      241 ttccgtgatc aatcttcagt ttagccttag gcataatgtc ccgcacggca acatagagga
      301 taaaaaacaa cgaacgggta aatatccgtt tcccgtcggg gtgcgaataa tcgatgaaac
      361 ggatatcttt aggtttcacc acctcgaaag ttaactctcg tactttatgg ttaaccattg
      421 ccccacaaat tgggaaaggc atttcgatgc ccatatcacc agcaatttcg agcagcgtag
      481 tgcctaaggg atattcttta tattgattgt tgttttcaca aaaaatttcg attgtcttca
      541 tgctataaat cttgaaaaat tacgctcgtg caactcttgc acgttttctt ttcaaaaata
      601 caaaaaaaga gatgcctgct aacttttaaa tgccagcaaa aggcaaaatg aaaaatcatt
      661 cctcagaaat gaagaacaga aaaacaataa aatttttaat tcacttattg tttaaaaacc
      721 ttctcaattc tatgtgttcc ttcagtagtt tgaattaaaa caacgtacaa tccttttctc
      781 aagttactgg tattaatcgt ttgccccgga ttcgtcaact cgcgcactaa ttggcctgac
      841 tgattgaata ttacaatcga ttcaacacaa tgagcattca taatgtatag ctcctcatca
      901 aatgggtttg gatagagagt tacgttatca aaaatgttaa aaccatcggt tcctgttgtg
      961 agattaacaa tttgggttga aaggactttg tacttattgt tatcttcaga tacaattccg
     1021 gttaccccat agttgtttcc ttcctctaac gagaaacttc ccaccaatga gttatcaacc
     1081 aaatagtttc cggcctgcca tgttcctgat gtattttttg tttcactaga tttcccggta
     1141 atataacata gaacaccttc gtgcttctca gccaacgatg aagtaataga aattggctgt
     1201 aattgaacaa cttgcaatat cgtttttatt tccgacacgt tgtcaattgt tgtcattcca
     1261 tttatctcag ctactgttcc aactatttca accgaaaggt tttgacttac aggaattgtc
     1321 gaccggacaa aaattccgca ccaaggagcg ttgtcatcct gcatgaagaa accatcagta
     1381 gtaatggcgg caattgttcc gcgaactttt accagcgtcc cgctgattgg tgaagaagaa
     1441 acttctccct gaatttgctt aattgattta agagaaagct cattcgatga aacaacaatt
     1501 acttcgttta cagcaaagct catggctccc gatgtttcta tggcgcaaac ataatacgtt
     1561 cccggcagca ggttctcggc attcataact acagaacccg gagaaagcag gttcgcctct
     1621 ctgcacttat tttgttggat tgcattttta atagaaatga catctttggg aacatcgttt
     1681 cggacaagat atactttcgc cgctttactg caagtcacag ttactgtacc agaagcatta
     1741 tttggtatcg ttaccgactg aacagaaaca atctcagacg aagagttacc ggtagagaac
     1801 atccaatatt ttggggtggg caaccctgag aatggattac ccgctaaatc agttatcaca
     1861 ccataaggaa tcgatacggt ataggtcatg ttttctttca gtgatggtgc ctgaatatct
     1921 atcctgttgt ttttgacaag catatcgcta agtgaaaaaa caaagtaatt gacaccaagt
     1981 atctgctcca tcgattggtc gctaagaatt atatacccat cggattttac aataatttga
     2041 tcgacacaaa attcgattga aattaccggg tttaatgaaa cagccgttgc accaggcttc
     2101 ggtgaaataa tgttgaaaca attgatgctt gataacgtac gcactgaaat tatatttgaa
     2161 tagctgctcg atccgttaag gttaatggct tttatccggt aaaaatattc agtgtttgtc
     2221 tccagaccgt cgagcgtaaa tgaacaaacg ttacctacat tcttattttc gaagcctgga
     2281 agaaaagatg aaaaactgct atttaatgat acatcgagaa aataaccggt agcattttca
     2341 gcccaattcc agttcgcagt aaacccttcg gcagaaacat cggtacctgc aattgcaact
     2401 ggaaccgaag gaaccgttgc ggcggatgta tgcaccataa cggcgttcga gtaattgctg
     2461 ctgccagcct cattatacgc cactacccgg taataatagc ttgtatttgc agtcaatcct
     2521 gtgattgaaa atgaagtaac atctccaaca ttcttatgtt gaaagcctgt aagaaaactt
     2581 gtgaaatcaa tattgtcaga tatatcgata taataccctg tagatccttc agtgtttccc
     2641 cagtttgcag taaagaaatc cattcctacc gataaagggg ccagggctat aggtgctgga
     2701 ggaggtgttc ctgccgaggg aataccatac accttattcg atgccgtact ttcgcctatc
     2761 tcgtatattg ctgacacata agcccaatac tttattccat tagatagtcc gctaagggta
     2821 acagacagat ctgttgtgtt ttcaacagca agggtgtaag cgccactctc agaattacct
     2881 agataaacat tgtatccaat tggggcaccg gcatcgggag tcatccaatt tattgccatt
     2941 ttaccgtttg ccgtcgtcgc tgtaacattg cttggaggat agagaacaag aggttctccg
     3001 ccttttacat caacggaata gtttcccaca actgaagaat ttactccctt aacctcaaaa
     3061 taataccagg tatcggcaaa caaactatac gtaatctttg cattcctgtt cgttccccca
     3121 tcattattaa aatgaagttg cgttgtggca tcgtaatcaa aaagggtcaa ataagtatct
     3181 agctctccat gggtttcaat ggtatagagc cccgaagtag tcgttttaaa cttatacgca
     3241 tctatttctc cgttgagaga aagtactgag ttataagtag gcgcattaac aatgattgga
     3301 tgatagccag gagtaactga aataatattc gaaatgggac tttcaccatt actgaacacg
     3361 gcttttatac aataccaat
//
LOCUS       DAOPUW010000138         3376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_28677_length_3376_cov_3.468835, whole genome shotgun sequence.
ACCESSION   DAOPUW010000138 DAOPUW010000000
VERSION     DAOPUW010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28677_length_3376_cov_3.468835"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1657
                     /locus_tag="PKJ43_03370"
     CDS             <1..1657
                     /locus_tag="PKJ43_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNZ71631.1"
                     /translation="EVAGNLVKVEDYTNKIGYSERTDVVIEPRLSAQWFIAMQKLAKP
                     ALDAVENDEIVFHPSKFKNLYRHWMSNVKDWCISRQLWWGHQIPVYYLADGSYVVAVT
                     ADEALELAKQKTGNNNLTINDLRQDEDVLDTWASSWLWPISVFNGISDPENDDIKYYY
                     PTNDLVTAPEIIFFWVARMIIAGYEFRGKMPFKNVYFTGIVRDKLRRKMSKSLGNSPD
                     PLDLIEQYGADGVRVGMLLSSPAGNDLLFDEALTEQGRNFSAKIWNAFRLVKNWDVDA
                     SLPQPEHSKLAVEWFSNKLSNVICEMDEHFARFRISDALMLIYTTVRDEFSSWLLEIV
                     KPAYQQPIDQKTYDEVIELFDKLMRLLHPFMPFITEEIWQLLLQRADRESIMVSLQPK
                     ASQVNPEMLENFEDVKEAVTGIRNIRKNNNIAFKDTIKLFVNEGSKGFVGYYNPILVK
                     LGNLSEIEMTSEDKKGAVSFLVKTSGFFVDLGEKVDVEAELKRLGEELAYTQGFLRSV
                     MAKLGNEKFVSGAPAQVVENERKKQADAEDKIRSIEERIASLK"
     gene            1883..>3376
                     /locus_tag="PKJ43_03375"
     CDS             1883..>3376
                     /locus_tag="PKJ43_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNZ71632.1"
                     /translation="MKRHLIFLIILCFTLSVAGQITTEPAFPVAGQSVKIIFDSKKEP
                     RLGAFSEDLYAHTGVGVEGDGNWQYVIGQWGQNATQPKLTFKGDGIYELVIRPSILDF
                     YNVPEGKQVVNLSFVFRNSAGNKQTNDLFVVVYQNGLNVDLLFPSNNFILQIDKQYTF
                     SAIAGSLANLKLYVDHELVGQNTGTEIHHDFSFNSAGEHMVLVVAESQDGQLVSDSAF
                     ICVSTGTPESPRPAGAQKGITYLSDQSLRLVLFAPNKENVFVLGDFNGWRPKNSFQMN
                     RDGDYFWIDIDGLIAGKEYAFQYLIDGELLIADPYSEKILDPWNDKYISNTTYPGLLQ
                     HSEEKIEQIATVVKAGQQPYQWEVNEFTIPAKNQLIIYELLIRDFTDEHSFQAVIDKL
                     DYLEDLQINVLELMPVNEFEGNSSWGYNPSFYFAPDKYYGPKDDLKRLVDECHKRGIA
                     VVIDMVLNHSYGQSPLVQMYWNPVNKRPSADNPWYNVVSNFQNPDAQW"
BASE COUNT          981 a          584 c          779 g         1032 t
ORIGIN      
        1 cgaagttgcc ggaaatcttg ttaaagttga agattatact aataaaatcg gctattcaga
       61 gcggactgat gtggttattg aaccccggtt atccgcgcaa tggtttattg ccatgcaaaa
      121 attggctaaa ccggcactcg atgctgttga aaatgatgag attgtttttc atccatcgaa
      181 attcaaaaat ctttaccgcc attggatgag caatgtaaaa gactggtgta taagtcgtca
      241 gctatggtgg ggacatcaaa taccagttta ttatttggcc gatggcagtt atgtagtggc
      301 tgttactgcc gacgaagctt tagaattagc aaagcaaaaa accggaaaca acaatcttac
      361 aattaatgat ttacgccagg atgaagatgt cctcgatact tgggcttcgt cgtggttatg
      421 gccaatttct gtttttaatg gaatttcgga cccggaaaat gacgatataa aatattatta
      481 tcctacaaat gacttggtaa ccgcacctga gattattttc ttctgggttg cccgtatgat
      541 tattgccgga tatgagttca gaggcaaaat gccttttaaa aatgtatatt ttaccggaat
      601 tgttcgcgat aaacttcgtc ggaagatgtc gaaatcgctt ggaaattcac cagatccgct
      661 cgacctgata gagcaatatg gcgcagatgg cgttagggta ggcatgctgc taagttcacc
      721 tgcgggcaac gatttgcttt ttgatgaggc acttaccgag cagggacgta atttttcagc
      781 taaaatttgg aatgctttcc gtttggtaaa gaattgggat gttgatgctt cgttgcctca
      841 accagagcat tcaaaacttg ctgttgagtg gttttctaac aaactgagca atgtgatttg
      901 tgaaatggac gagcactttg cccgctttcg tatttcagat gcattaatgc ttatatatac
      961 cacggttcgt gatgaattct catcgtggct gctcgaaatt gttaagcctg cttaccagca
     1021 acccattgac cagaaaacgt acgatgaagt aatcgagctt ttcgataagt tgatgcggtt
     1081 attacatcct ttcatgccat ttattactga ggaaatttgg cagttgttgt tgcaacgtgc
     1141 tgacagggag agtatcatgg tttcgttgca gcccaaagct tcacaggtaa atccggaaat
     1201 gctcgaaaat tttgaagacg tgaaggaagc tgtaaccgga atacggaata ttaggaaaaa
     1261 taacaatata gcgtttaaag atacgattaa gttgtttgta aacgaaggca gtaaaggatt
     1321 tgttggctat tataatccta ttttggttaa gcttggaaac ctctctgaaa tagaaatgac
     1381 cagcgaagac aaaaaaggcg ctgtttcatt ccttgttaaa acctcaggat tctttgttga
     1441 tttgggtgaa aaagttgatg ttgaagcaga actgaaaagg ctaggagagg aattggcata
     1501 tactcaaggg tttttaagat cggttatggc aaaacttggg aacgagaaat ttgttagcgg
     1561 tgcgccagct caagttgttg aaaacgaaag gaaaaaacag gctgatgctg aagataaaat
     1621 ccgttctatt gaagagcgta tagcttcatt gaagtaacag ttacaagcaa taatctaaaa
     1681 ctccttcggt gttacttatt aagtgaatgc cggaggagtt ttcatttttc ctgttgtaaa
     1741 acatttttct gcaaacgttt gcgatttcgt tttcggtgtt tttttgctct cgggcattgc
     1801 tttgccaccg caagcaatta taaaatggct taattttaca gggctagtac aggattttgt
     1861 ttagcagata ataaattaag ctatgaaaag acaccttatt tttctaataa tactttgctt
     1921 taccttgagt gttgccgggc aaataactac cgagcctgcc tttcctgttg ccggtcagtc
     1981 ggtaaagatc atcttcgatt caaaaaagga acctcgcttg ggagcttttt ccgaagattt
     2041 gtatgcacat acgggtgttg gcgttgaagg agatgggaat tggcagtatg ttataggaca
     2101 gtggggacaa aatgctaccc aaccaaaatt aacatttaaa ggcgatggga tttatgagtt
     2161 ggtcattagg cctagcatac tcgatttcta caatgttccg gaaggaaagc aagtagtgaa
     2221 tctttcgttt gtttttcgta attcggctgg taacaagcag actaatgact tatttgttgt
     2281 ggtttatcag aatggattga acgttgattt gctatttcct tcaaataact tcatccttca
     2341 gattgataag caatacactt tttcagctat tgcaggttcg ttagccaatt taaagctata
     2401 tgttgatcat gagctcgtcg gacagaatac tggtacggaa attcaccacg atttctcgtt
     2461 caattctgca ggagaacata tggttctcgt tgttgctgaa agccaagatg ggcaactcgt
     2521 ttctgattct gcatttattt gtgtttcaac tggaacacct gagagtccgc gaccagcggg
     2581 ggcacaaaag gggataactt acttgagtga tcagtcgtta aggcttgtgc tttttgcacc
     2641 gaataaagaa aatgtgtttg ttttgggaga tttcaatggt tggcggccaa agaattcttt
     2701 tcaaatgaac cgtgatggcg actatttttg gatagatatt gatggattaa ttgctgggaa
     2761 agagtatgct tttcagtatc ttatcgatgg ggagttactt attgccgatc catattcaga
     2821 gaagattttg gatccatgga atgataaata cattagtaac acaacttatc ccgggttgct
     2881 gcaacactct gaggagaaga ttgaacaaat agccacggtg gtaaaagccg gtcaacagcc
     2941 ttaccaatgg gaagtgaacg aattcactat tcctgcaaaa aatcagttga ttatttacga
     3001 attgcttatt cgcgatttta ctgacgaaca ttcatttcag gcggtgatcg ataaactcga
     3061 ttatctggaa gacttgcaga ttaacgttct tgaacttatg ccggtcaatg agttcgaagg
     3121 gaatagtagt tggggctaca atccttcgtt ttattttgcc cctgataaat attacgggcc
     3181 taaagacgat ctgaaacgac ttgtagacga gtgccataaa agaggcatag ccgtagtgat
     3241 cgacatggtg ctgaaccata gttatggaca gagtccgctg gttcaaatgt attggaatcc
     3301 agtgaacaaa cgaccgagtg ctgataatcc atggtataat gttgtttcga attttcagaa
     3361 tcctgatgcg cagtgg
//
LOCUS       DAOPUW010000139         3370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_28753_length_3370_cov_4.290799, whole genome shotgun sequence.
ACCESSION   DAOPUW010000139 DAOPUW010000000
VERSION     DAOPUW010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28753_length_3370_cov_4.290799"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="PKJ43_03380"
     CDS             complement(<1..1182)
                     /locus_tag="PKJ43_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNZ71633.1"
                     /translation="MAIKRSKIFIIDTNVILHDHRCLFNFEENDVVLPITVLEELDKF
                     KRGSEQLNYNAREFVRLLDELVKDDLFNGGLPLGKGKGKLKIETGKTFSEKLKTSFRE
                     DIPDHRILAITEWVKENNPKQKVVLITKDINLRMKAKSLGLDAEDYKNDKVVNTEIFD
                     RGVNVRDKFDDVLISRLYEDRFLPLADACFDKDPTANDYFIFRGINASALVRYDAGAR
                     HFERVDKKSAYGIKPRNAEQTFLLNALLNPEMKLVAVTGKAGTGKTLLALAAALAQYD
                     IYEQIMLARPIVALSNRDIGFLPGDADEKIRPYMQPLFDNLAVIKHCFNPRSVEYSRI
                     DEMEKEERLVIAALAYIRGRSLSNSFFIVDEAQNLTPHEIKTIITRAGEGTKMVFTGD
                     LH"
     gene            1317..2630
                     /locus_tag="PKJ43_03385"
     CDS             1317..2630
                     /locus_tag="PKJ43_03385"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNZ71634.1"
                     /translation="MDYQQTIHYLYENLPMFHRIGPAALKNDLSNTKTLDNAYGFPHQ
                     HFKTVHIAGTNGKGSVSHMLAAIFQSAGYKTGLFTSPHLIDFRERIKVNGEMIPENDV
                     VNFVSDFIERNKILNLQASFFELTTIMAFDFFRKEKVDIAIVEVGLGGRLDSTNIITP
                     ELSVITNISFDHTAILGHTLDKIASEKAGIIKTGVPVIIGESHPKTCNVFLEKASQTG
                     SPITFADEEFLSKTQKNGELSIFSHYKKMYENITPELKGDYQQKNILTVIAAIEVLKE
                     KINITNEAVRNGIAQVTSLTGLQGRWQQLCETPRIICDTGHNEAGMRWVAGQLKREKY
                     NRLHIVFGMVSDKDTSSVLALLPTSADYYFTKAPIERALDEINLKEKANSFGLNGNAY
                     PTVKEALDAAKKRAGKNDLIFVGGSTFIVAEILTNNNTCQPLKSQ"
     gene            2665..3327
                     /locus_tag="PKJ43_03390"
     CDS             2665..3327
                     /locus_tag="PKJ43_03390"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ71635.1"
                     /translation="MKLTVHPIYKLIAQGEHQQQDFKYCINDSRKIARSLVAFANSDG
                     GRLLVGVKDNGKIVGVSSEEEYYMVDAAARIYSKPKIEFTAQQWDVDGKTVFEVTVAP
                     SRQRPYFAQSDDGKWLAYIRKDDENILANNVLLKSWELNKKTTGLCFQYDEPRKIIIE
                     YLNHNQSITLTKFTRLAQITRGYAENILAELLAMKCIDGNFDQIPVTYQLNGEFDITS
                     LK"
BASE COUNT         1068 a          723 c          628 g          951 t
ORIGIN      
        1 gtgcaaatcg ccggtaaaaa ccattttggt cccctcgcct gcccgtgtaa ttatcgtctt
       61 tatttcgtgt ggagtaaggt tttgtgcttc atcaacaata aagaaagaat tcgacaaact
      121 tcgtccccga atgtaagcaa gtgcagcaat cactaaacgt tcttcttttt ccatttcatc
      181 aatacgggaa tattctacac tgcgtggatt aaaacagtgt ttgattacgg caagattatc
      241 gaaaagcggc tgcatataag gccttatttt ttcatcggca tcgccgggca agaaacctat
      301 atcgcggtta cttaaggcaa caattggcct tgcaagcata atttgctcat aaatatcgta
      361 ttgagccaaa gctgcggcta atgccagcag tgtttttccg gttcctgctt ttccggtaac
      421 tgccactaat ttcatctcag ggttcagcaa agcattcagc agaaaagttt gttctgcgtt
      481 gcggggcttt atgccgtatg cactcttctt gtcaacccgt tcgaaatgcc gggccccagc
      541 atcgtaacgc acaagagccg aagcatttat tccccggaat ataaaataat cattagctgt
      601 aggatcttta tcgaaacagg catcggccag tggcaaaaaa cgatcttcgt ataatcttga
      661 gattaaaaca tcatcaaatt tatcgcgaac attaactccc cgatcaaaaa tctcggtatt
      721 gactacctta tcgtttttat agtcctctgc atcgagccct aacgatttag ctttcatccg
      781 caggttgata tctttggtaa tgagcactac tttttgtttc gggttattct ctttcaccca
      841 ctcggttatt gccaatatcc tgtgatcggg aatatcttca cgaaaagaag tttttaattt
      901 ttcagagaaa gttttcccag tttcaatttt cagttttccc ttcccttttc ccaacggaag
      961 gccgccatta aataaatcgt ctttcaccaa ttcatcgagt aagcgcacaa attccctggc
     1021 attataattc aattgttcgc ttccgcgctt aaatttgtcg agttcttcaa gaaccgttat
     1081 gggcaacacc acatcgtttt cttcaaaatt aaacaagcag cggtggtcgt gtagaattac
     1141 attggtgtcg attatgaaaa tttttgatcg tttaatggcc atattttcat tttttttggg
     1201 ttctttgtag aaaaacaaga ttgaaataat cttttaaatg tataaaattt tacgtgttag
     1261 attgttaaca tagacccgtt ttatcctatt tttatatcaa cgatttgtta ataacaatgg
     1321 attatcagca aaccatacat tacttatacg aaaacctgcc catgttccac cgcattgggc
     1381 ctgcagcact taagaacgat ttatcgaata ccaaaaccct cgataatgcc tacggttttc
     1441 ctcaccagca ctttaagact gtccacattg caggcaccaa cggcaaaggc tctgtttcgc
     1501 acatgttagc agctattttc caaagcgcag gatataaaac agggctattt acatcgcccc
     1561 acttgatcga tttcagagaa cgcattaaag ttaatgggga aatgatcccc gaaaacgatg
     1621 tcgtaaattt tgtcagcgac tttatcgaaa gaaacaaaat actaaacttg caagcttcgt
     1681 tttttgagct aactactatt atggcctttg atttctttag aaaagaaaaa gtagatatcg
     1741 ccattgttga agtgggcctt ggtggccggc tcgattctac aaacatcata acccccgagc
     1801 tttcagtcat caccaacata agttttgacc acacagccat tttaggccat accctcgaca
     1861 aaattgcctc cgaaaaagcg gggatcatca aaactggagt tccggttatc ataggcgaat
     1921 cacacccgaa aacctgtaat gtttttcttg aaaaagcatc gcagacaggt tccccaataa
     1981 catttgccga tgaagagttt ttatcgaaaa ctcaaaagaa cggagaactg tctattttca
     2041 gccattataa aaaaatgtac gagaacatta ctccagagct gaaaggtgat tatcaacaaa
     2101 agaatatttt aacagtaatc gccgctatcg aagttctcaa agagaaaata aacatcacca
     2161 atgaagccgt tcgaaatgga atagctcaag taacaagcct taccggacta cagggccgct
     2221 ggcagcaact ttgtgaaact ccccggataa tatgcgatac cggccacaac gaagccggaa
     2281 tgcgctgggt ggctggtcag ttaaaacgag aaaagtataa tcgcctgcat atcgtttttg
     2341 gcatggttag cgacaaagac accagctctg tgttagcttt attacccaca tcggctgatt
     2401 actatttcac taaggccccc attgagaggg cattagatga aattaatctc aaggagaaag
     2461 cgaactcttt tggattaaat gggaatgcct atcctaccgt aaaagaagca cttgatgctg
     2521 caaaaaaacg agcaggaaaa aacgacctca ttttcgtggg gggtagtaca tttattgttg
     2581 ccgaaatact gaccaataat aacacttgcc agccactaaa aagccaataa gctactgata
     2641 tttcacatta taagactcaa aaacgtgaaa ctaactgtac acccaatata taaacttatt
     2701 gcacaaggcg agcatcagca acaagatttc aaatattgca ttaacgactc tcgcaaaata
     2761 gcccgttctc tcgttgcttt tgcgaattct gatggaggac gtctgcttgt tggtgtaaaa
     2821 gacaacggaa aaattgttgg ggtaagctct gaagaagaat attatatggt tgatgctgcc
     2881 gccagaattt acagtaaacc aaaaatcgaa tttacagccc aacaatggga tgtcgatgga
     2941 aaaaccgtat tcgaagtaac tgtagcgccc agccgtcagc gcccatactt tgcccagtct
     3001 gatgacggga aatggcttgc ttacataaga aaagacgacg aaaatatatt ggcaaataat
     3061 gttctattga aatcatggga attaaataaa aaaaccactg gtctctgttt ccaatacgat
     3121 gagcccagaa aaatcataat cgaatatcta aatcataacc aaagcattac cttaacgaaa
     3181 tttacaaggt tagcacaaat tacaagaggc tatgccgaaa atatcctggc agaattgctc
     3241 gcgatgaaat gtatcgacgg aaattttgat caaattcctg tcacatatca acttaacggg
     3301 gaattcgata taaccagttt aaaatagccg caaaaaagac attgattaca ctaatctttt
     3361 agtctaacaa
//
LOCUS       DAOPUW010000140         3341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_29107_length_3341_cov_3.512781, whole genome shotgun sequence.
ACCESSION   DAOPUW010000140 DAOPUW010000000
VERSION     DAOPUW010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29107_length_3341_cov_3.512781"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PKJ43_03395"
     CDS             <1..210
                     /locus_tag="PKJ43_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71636.1"
                     /translation="NRNDSFNTYSAGLGYRQQSFFIDFAYRLSDISTAYKVHEMVPND
                     PMNGKNIAQLNYTNSQATITLGFRF"
     gene            252..623
                     /locus_tag="PKJ43_03400"
     CDS             252..623
                     /locus_tag="PKJ43_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71637.1"
                     /translation="MRGEYPLLVCIITIKIDYTKHIKNPLSMNVARPIDYQSACNYFK
                     EAEKKGEKLLMGDINTNFWLHKERINLDFVRDTARKFTDARIFIIAEGKNKGFYMYSE
                     TSKTCFLLFDIASKIPHAQFA"
     gene            649..1371
                     /locus_tag="PKJ43_03405"
     CDS             649..1371
                     /locus_tag="PKJ43_03405"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNZ71638.1"
                     /translation="MTINPEFDPLGQAIYNYHFHNDNTPVIVQSDVIEDEELPPDYFF
                     RKYNEMPLLERLALKHSHGKILDIGAGAGCHSMHLQEKGFDVTSLDVSELSCNVMQHR
                     GLKKVINNDILSLNHCKFDTILLLMNGIGIAKDLKGLSVLLKHLKKILEPGGTILVDS
                     SDLIYLYEEEDGSILFDINSANYYGEIEYKMKYRKIIGETFSWLFADNVILFDIATKN
                     GFDAKLLEYGPHYDYLAELKLK"
     gene            1374..>3341
                     /locus_tag="PKJ43_03410"
     CDS             1374..>3341
                     /locus_tag="PKJ43_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HNZ71639.1"
                     /translation="MNYRNLSSLEIEQLEKQLCTCTEWNNILVSNNFNPKNIFNTHFS
                     GTIKIGKQEKNIELFGGISKPSGINNSTLHNVIIGDNCYINNIKTHISNCEIEENVII
                     DNVQMIATQGPSTFGQGQKVAVLDESGGRKIMISDNLSAQIAYIMTLYRYRPLLIENL
                     EKIINVYVETKKSTFGHIGKNTTLINCGSLKNVWLGEHVTIEGARALEDGTIISNEKA
                     PVYIGNNVIMKHFVVQSGTSIIEGVIIDKCYVGQGCELGKNYSAENSVFFANCQGFHG
                     EACSIFAGPFTVTHHKSTLLIAGMYSFLNAGSGSNQSNHMYKLGPIHQGIVERGSKTT
                     SDSYLLWPAKIGPFTLVMGRHYKNSDTSDMPFSYLIENDDKSWLVPGINLRSVGTIRD
                     AMKWPKRDNRKDPNKLDCINFNLLSPYTIQKMVKALEILENLKKVSGQTSQEYVFHNA
                     SISQSALNRGIMLYNIGIDKFLGNSLITRLFKANLKTEEDLRQRLRPDCEDGQGEWVD
                     ISGLIAPKNEVNKILEAIENGDLCDLQAIQQEFQKLHSRYYEIEWTWAAALLEWRLQK
                     PIEQMTICDIMNLVSRWKKSVIDLDQMLYEDARKEFNLTSMTGFGQDSNDVSTQQADF
                     EMVRGSFESNSFVQEIKSHIDKKSNLGERMMN"
BASE COUNT         1151 a          641 c          630 g          919 t
ORIGIN      
        1 aaccgaaatg atagcttcaa tacctattcg gcaggattgg gataccgtca acaatcattt
       61 ttcatcgatt ttgcctatag actaagcgac atcagtacag catacaaagt acatgaaatg
      121 gtgcccaatg acccaatgaa cggtaaaaat attgcacaat taaattatac caacagtcag
      181 gcaacaatta cactgggctt tcgtttttaa tgtgagaggt tgttcaaaaa aatgttgata
      241 acttttttaa catgaggggg gaataccccc ttcttgtttg tattattact atcaagatcg
      301 actatactaa acacataaaa aacccattaa gtatgaatgt agccaggcca atcgattatc
      361 agtcagcctg caattatttc aaagaggcag aaaagaaggg cgagaaactt cttatggggg
      421 atattaatac caatttttgg ctacataagg aaagaattaa tctcgatttt gtaagagata
      481 ctgcacgaaa atttactgat gccagaatat ttatcattgc tgaaggaaaa aacaaaggtt
      541 tttacatgta ctcagaaaca agcaaaacat gctttctcct tttcgatata gcctccaaaa
      601 taccccatgc acaatttgca taaagccaat aggtaacatt ataataaaat gacaatcaat
      661 cccgaatttg acccgctggg acaagccatt tacaattatc actttcacaa tgacaacact
      721 cctgttatcg ttcaatctga tgtaattgaa gacgaagaac ttccccctga ttactttttc
      781 aggaaatata acgagatgcc tctactcgaa aggcttgcac tgaagcatag ccatggcaaa
      841 atattagaca ttggtgccgg cgccggatgt cattcgatgc acttgcaaga aaaaggattt
      901 gatgtaacat cactcgatgt ttcagaatta agttgtaatg ttatgcaaca ccggggcctt
      961 aaaaaagtca ttaataacga cattctttct cttaaccact gcaaattcga cacgatatta
     1021 ctgctgatga atgggatcgg aattgctaaa gacttaaagg gactttcagt attacttaag
     1081 cacctgaaaa aaatactaga gcccggtgga accatccttg tcgattcttc agacctgatt
     1141 tacctttatg aagaagaaga cggatcgata ttgttcgaca ttaattcagc caattattac
     1201 ggcgaaattg aatataaaat gaaatataga aaaattattg gcgaaacctt ttcgtggtta
     1261 tttgccgata atgtaatttt gtttgatatc gcaacaaaaa atgggttcga tgccaagttg
     1321 ttggaatacg gacctcatta tgattatctg gctgaattaa aacttaaatg actatgaact
     1381 atcgtaatct ctcttcctta gaaattgaac aactagaaaa acaattatgc acttgcactg
     1441 aatggaataa cattttggtt tccaataatt tcaaccccaa aaatattttc aacacccatt
     1501 tctcgggtac cataaaaatt ggaaagcagg aaaaaaacat tgaacttttt ggcgggattt
     1561 ccaaaccatc tggaataaat aactctaccc tccacaatgt tatcattggc gacaattgct
     1621 atatcaataa tataaaaacc cacatttcga actgcgaaat tgaagaaaac gtaatcatcg
     1681 acaatgtgca aatgattgcc acacaaggtc caagtacttt tggacaaggg cagaaagttg
     1741 ccgtactcga tgaatcgggt ggccggaaaa taatgattag cgacaacctt tcggcacaaa
     1801 tagcctatat tatgacctta tacaggtatc ggcccctttt gattgagaac cttgaaaaaa
     1861 taataaatgt ttatgttgaa acaaaaaaat caacatttgg tcatatagga aaaaacacga
     1921 ccttaattaa ttgtggttca ctaaagaatg tttggcttgg cgagcatgtc acaattgaag
     1981 gcgcacgcgc ccttgaagat ggtacaatta ttagcaatga aaaagcacca gtttatattg
     2041 ggaacaacgt tataatgaaa cactttgttg tacaatcagg tacttcaatt atcgaaggag
     2101 taatcattga caaatgctat gttggccaag gctgtgaact cggtaaaaat tactcggctg
     2161 agaattcagt ctttttcgcc aattgccaag ggtttcacgg cgaagcatgt tcaatatttg
     2221 cgggtccatt tactgtaacc caccataaat cgaccttgct aatagccgga atgtattcct
     2281 ttttaaatgc aggatcgggt tcaaaccaaa gtaaccacat gtacaagcta ggccctattc
     2341 atcaaggaat tgttgagcgc gggtctaaaa ccacctccga ttcttatttg ctttggcctg
     2401 ctaaaattgg cccatttacc ctggttatgg ggcgccatta taaaaattca gacacatcgg
     2461 atatgccatt ttcgtattta atcgaaaatg acgacaagtc gtggctcgtg ccgggaatca
     2521 acctccgaag cgtgggtaca attcgcgatg caatgaaatg gcccaaacga gataaccgga
     2581 aagatccgaa caagctcgac tgcatcaact ttaacctact aagcccttac acaattcaaa
     2641 aaatggttaa agcccttgaa attctcgaaa accttaaaaa agtatcaggt caaacttcgc
     2701 aagaatacgt gttccataat gcttcaattt cgcagtctgc gcttaacagg ggaattatgc
     2761 tctataatat cgggattgac aaatttcttg gcaactcgtt aattacaagg ctattcaaag
     2821 ccaaccttaa aacagaagaa gaccttcgcc aaaggttgag acctgattgt gaagacggac
     2881 aaggcgagtg ggttgatata tcgggcttaa ttgctcccaa aaatgaagtc aacaaaattc
     2941 ttgaagccat tgaaaacgga gatttatgtg atcttcaagc cattcagcaa gagtttcaaa
     3001 agttacattc aagatactac gagatcgaat ggacatgggc tgctgcatta ctggaatgga
     3061 gactacaaaa gcccattgaa caaatgacta tttgcgatat catgaacctt gtgtcgcgat
     3121 ggaaaaaaag tgtaatagat cttgaccaaa tgctatatga agatgcccgc aaagaattca
     3181 atttaacatc gatgactggt tttggtcaag acagcaacga tgtaagtact caacaagcag
     3241 attttgaaat ggttcgtggt agttttgaaa gcaatagttt tgtccaagaa ataaaatcgc
     3301 acatagacaa gaaatcgaat ttgggcgaac gtatgatgaa c
//
LOCUS       DAOPUW010000141         3340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_29118_length_3340_cov_3.474581, whole genome shotgun sequence.
ACCESSION   DAOPUW010000141 DAOPUW010000000
VERSION     DAOPUW010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29118_length_3340_cov_3.474581"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /gene="speA"
                     /locus_tag="PKJ43_03415"
     CDS             complement(<1..804)
                     /gene="speA"
                     /locus_tag="PKJ43_03415"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HNZ71640.1"
                     /translation="MRKWRIEDSAELYNINGWGVDYFSINEDGHIIVTPKKECASVDL
                     KEIIDELSLRDVTTPILIRFPDIIDNQIDTISQCFNRAAIEYGYKAQNHIIYPIKVNQ
                     MRPVVEEIVSHGKKYNIGLEAGSKPELHAIIAINTDPESLIICNGYKDEDYIELALLA
                     QKMGKRIFIVVEKLNELKLVASISKQLKIKPNIGIRIKLASSGAGKWESSGGDVSKFG
                     LSSSELLEACDFIQKNKLEDCFKFIHFHIGSQITKIRRIKNALREASQFY"
     gene            complement(912..2417)
                     /gene="zwf"
                     /locus_tag="PKJ43_03420"
     CDS             complement(912..2417)
                     /gene="zwf"
                     /locus_tag="PKJ43_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNZ71641.1"
                     /translation="MKKTSPHIVVIFGASGDLTKRKLIPALFNLHQQNLLGHPFAILG
                     VSRTEMTDTMFREKMMEFLPDNETSADFIGHLYYESLQTSDEADYPRLKERIDDLRRE
                     INAPANILFYLSTPPNLYPLIPKHLANVGLNNESDGFKRLIIEKPFGSNLESARHLNR
                     DLLQYFEEDQLYRIDHYLGKETVQNLLVLRFSNSLWEPLWNHKYIDHVQITSSESLGV
                     EKRGGYYDHSGALRDMVQNHLLQMLAMVAMEPPTVINADAIRNEMLKVFQSIRPLKKE
                     NLHEDVVRGQYTSSHIKGEAVAGYREEEGVDPESRTETFVAMRLNIDNWRWGGIPFYI
                     RTGKKLPTRVTEIVINFKPTPHHLFDAALNGTTAPNQLIIRIQPDEGILMKMGLKVPG
                     AGFMVQTVNMGFHYSDLSDAYVPTAYERLLLDCIHGDATLYSRGDNVEKAWEIVQPII
                     DEWDTNPDFPLHGYPAGTWGPLATDELMAAHPSGWRYPCKNLNNDGEYCEL"
     gene            complement(2510..3142)
                     /locus_tag="PKJ43_03425"
     CDS             complement(2510..3142)
                     /locus_tag="PKJ43_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNZ71642.1"
                     /translation="MPLSATIQKGNNTLALWTITEPVSFFENNVNLSPDELQIYRCFK
                     NESRKREWLAVRCLLQETLKMKPQIEYSETGKPFLKNYPSKLSISHSRKMAGILLSNN
                     KLTGIDIEEITRPIDKVASRFLSESERMLIEKSGLEYGKILSWCTKEAIFKAMNHCNV
                     DFSTQIIIEKITPDSNIEARFIDHGVDIKIKLEYLLTDQHAVVWTSTNDM"
     gene            complement(3143..>3340)
                     /locus_tag="PKJ43_03430"
     CDS             complement(3143..>3340)
                     /locus_tag="PKJ43_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HNZ71643.1"
                     /translation="KVYGTVYSIKGNAASPMQFYLTDSVEHFLRGSMYISEIPNYDSL
                     QPVINFLEADMIRLIETLSWK"
BASE COUNT          846 a          658 c          724 g         1112 t
ORIGIN      
        1 atagaattgc gaagcttcgc gaagggcgtt ttttatcctc cgtatttttg taatctgact
       61 acctatgtga aaatgaatga atttgaagca gtcttcaagt ttgtttttct ggataaagtc
      121 acaagcttcg agaagttcac tcgacgaaag tccaaattta ctcacatcgc cgcccgagct
      181 ttcccatttt cctgcgccag aactggctag tttgatccga atgccaatat tgggctttat
      241 tttcagttgt ttgctgattg acgcaacgag ttttagttca ttaagttttt caactacgat
      301 gaaaatacgt tttcccattt tttgagctaa cagggctagt tcaatgtaat cttcgtcttt
      361 gtagccgttg caaatgatta acgattctgg gtcggtattt atggcaataa tggcatggag
      421 ttcgggcttc gaaccggcct ctagtccaat attgtatttt tttccgtggc ttacaatttc
      481 ttcgaccacg gggcgcatct gattcacttt aatcggataa ataatgtgat tctgagcttt
      541 gtaaccatat tctattgccg cacggttgaa acattgcgag atggtgtcga tttggttatc
      601 gataatgtca gggaatctaa ttaaaatagg ggttgttaca tcacggagtg aaagttcatc
      661 gatgatttct tttaaatcga ccgatgcgca ctcttttttt ggggtaacaa taatgtgccc
      721 gtcttcgtta attgaaaaat agtcaactcc ccagccgtta atgttataaa gttctgcaga
      781 gtcttcaata cgccattttc tcatttcaaa ttaccagatt ctaagtgtga ttaataattg
      841 taaataaaac tgctacgaaa atagcaatga tatttgttta agctggttta ttttgagaaa
      901 aagttgtttt gctaaagttc gcagtattct ccgtcgttgt taaggttttt acaagggtat
      961 cgccaaccgc tcggatgtgc tgccataagt tcgtcggtag ccagtggtcc ccaagttcct
     1021 gccggatagc cgtgtaatgg gaagtcgggg ttagtatccc actcatcgat aattggttgc
     1081 acaatttccc atgctttttc aacattgtcg cctcgcgagt aaagtgtggc atcgccgtgt
     1141 atgcagtcga gcagcaaacg ctcgtaagct gttggtacat atgcatccga taggtcagaa
     1201 taatgaaatc ccatgttaac tgtttgtacc ataaacccgg ctcctggtac ttttagtccc
     1261 attttcatca ggattccttc gtcaggttgt attctgataa ttagttgatt tggggcagta
     1321 gtgccattaa gggccgcgtc gaaaagatgg tgcggtgtag gcttaaaatt aatcactatt
     1381 tctgttaccc ttgtaggaag cttttttcct gttcgaatat aaaaaggaat tccgccccag
     1441 cgccagttgt caatattaag tcgcatggcc acaaaggttt cggtacgact ctcagggtca
     1501 accccttctt cttcgcgata ccctgcaact gcttctcctt taatgtgtga cgatgtgtat
     1561 tgcccccgga caacatcttc gtgaaggttt tcttttttta atggtcttat cgattggaaa
     1621 accttaagca tttcgttgcg tattgcatcg gcattaatca cggttggtgg ttccatggca
     1681 accatggcca gcatttgcag caggtggttt tgtaccatgt cgcgaagcgc acctgagtga
     1741 tcgtaatagc cgccacgttt ctctactcca agcgattccg atgaggtaat ttgtacgtgg
     1801 tcaatatatt tatggttcca taatggttcc cataagctat ttgaaaatct cagtaccaac
     1861 aggttttgca cggtttcttt tccgaggtaa tggtcaatgc ggtacagttg gtcttcttca
     1921 aaatattgga gcagatcgcg attgaggtgg cgggcacttt ctaaattact cccaaatggt
     1981 ttttcaatga tgagccgttt gaagccatcg ctttcgttgt ttagtccaac attagccagg
     2041 tgtttgggaa tcaatggata aaggttgggt ggggtagaga ggtaaaacaa tatattcgcc
     2101 ggggcattta tttcgcggcg caaatcatcg atccgttctt ttagtcttgg ataatcggct
     2161 tcatctgatg tttgcagcga ttcataatac aaatggccta taaagtcggc actcgtttca
     2221 ttatcgggaa gaaattccat cattttctcc ctaaacatgg tatctgtcat ttcggtgcgg
     2281 cttacaccaa gaatggcaaa agggtgccct aacaaatttt gctgatggag gttgaacaat
     2341 gcaggaatga gcttgcgctt ggttaaatcg cccgatgccc caaatattac cactatgtgc
     2401 ggactcgttt ttttcatttt aatcgatgtt tatttcaatc cgagatacgt gtcgttacta
     2461 aatctaaaca aaaataacag aaaacctatt gatttgttgg tgatacttat tacatatcat
     2521 ttgtggaagt ccatacaact gcatgttgat cggttagtag gtattccagt tttattttta
     2581 tgtcgacccc gtggtcaata aagcgggcct cgatattgga gtcgggtgtt attttctcaa
     2641 taattatttg ggttgaaaaa tcgacattgc agtggttcat tgctttaaat attgcttctt
     2701 tggtacacca gcttaagatt ttgccatatt ctaagcccga tttttcaatc agcattcgtt
     2761 cgctttctga aaggaagcgc gaagcgactt tatcaattgg tcgggtaatt tcttctatgt
     2821 caattccggt taatttgttg tttgatagaa gtatgcctgc cattttccgt gagtggctaa
     2881 tgcttagttt gcttgggtaa ttttttaaaa atggtttacc agtttcagaa tattcaattt
     2941 gtggtttcat tttcaaagtt tcctgaagca aacaccgtac ggcaagccac tctcttttac
     3001 ggctttcgtt tttaaaacaa cggtatatct gcaactcgtc aggcgagagg tttacgttat
     3061 tctcgaagaa actaaccggc tctgttatag tccataaagc cagtgtgttg ttgccttttt
     3121 gtatggttgc agaaaggggc atttatttcc aactgagggt ttctatcagg cgaatcatat
     3181 cggcttcgag aaagttgatt acaggctgaa gtgagtcgta attcggtatt tctgaaatat
     3241 acatcgaacc acgcaaaaag tgctctacgc tatcggtaag gtaaaactgc atcggagaag
     3301 ctgcgttgcc tttaatgctg tacaccgttc catatacctt
//
LOCUS       DAOPUW010000142         3326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_29293_length_3326_cov_3.047080, whole genome shotgun sequence.
ACCESSION   DAOPUW010000142 DAOPUW010000000
VERSION     DAOPUW010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29293_length_3326_cov_3.047080"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="PKJ43_03435"
     CDS             <1..1134
                     /locus_tag="PKJ43_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71644.1"
                     /translation="NNTLQVSTRYSNRINNNLRIVAGIEYKHMFYDMKDDFRYDDDFR
                     WRTNIDMEENTGMLESFVNFKYRVVKNVNIVAGLHYTNFLLNNSQALEPRAALKWDIT
                     PKSSLNFGYGKHSMPENIVTYFANVYDDNMVASTPNKNLGLTKANHFIVGFDQRLTKN
                     MKAKIELYYQGLYDMPVENLDTSNFSIVDPYSNNIIPLVNEGEGRNYGAEFTLERFFD
                     NSYYFLVTGSLYKTEYKTLTNVWHNTVFDGTYAFNFLAGKEFILGKPEKGNTLNLNTR
                     VVYNGGRRNLAVNVEESRKNGYAVYDYSNPYEDRLNNIFQVNFTASLKLNRKNVTHEL
                     LLDFFNITNNQSKIREYYNTISKDRDYEKQLGIMPNVMYRIHF"
     gene            1418..2461
                     /locus_tag="PKJ43_03440"
     CDS             1418..2461
                     /locus_tag="PKJ43_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71645.1"
                     /translation="MKTQILALMLLLLSLSTAFAQKDSLKKNELVMYSFIVNRTPDNF
                     NIPLIGFINIANGNHKSAEVGFVNYTKGNFTGGQISFINTVGENCIGSQIGFVNTTVK
                     KFKGSQIGFVNTTLGDFFGSQVGFVNTSAKKSDNIQIGFVNTCGDSLRGAQVGFVNTS
                     LKRTVAPQIGLINVAKSLKGTQIGLVNFVDSIESGVPLGLLSIVRKGGYHAIELSVNE
                     MYPLNLSYKIGIPELYTSFVISYNPKDEKNVAIGAGLGSIIPLGKRLCFNPEFTSQTL
                     FFNEFQQYTNASANIGYKLNNHVMISAGPSLTYQYNYNKNSLNEPLFTFSESNWRNGT
                     FITGARVAVRYKF"
     gene            2595..>3326
                     /locus_tag="PKJ43_03445"
     CDS             2595..>3326
                     /locus_tag="PKJ43_03445"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNZ71646.1"
                     /translation="MKRTLLTRYFLVTFFWSWIIWISTALLCKAGVIGDQPADKVIKT
                     VVLVLGAFGPLVGALVAVRSEKNKGGVGLYLKRFLSFNIGYKAFIFPLLILCGTTLLA
                     WFLPSLFGYQKISMLLPSAWVFVPYLIAMMLFGGGQEEFGWRGYALPKLEENLGYWKS
                     NILLGTIWAVWHLPLWFIPGTSQQLMHFVGFMMLTIGYSFLFSWFRKLAGEKPFSGIY
                     VHGLANAFIPLFPILNMQPGAPQPAF"
BASE COUNT         1032 a          668 c          667 g          959 t
ORIGIN      
        1 aacaacaccc ttcaggtttc aacccggtat agtaaccgca tcaacaacaa cttgcgcatt
       61 gttgccggga tagaatacaa acacatgttt tacgatatga aagacgattt ccgctatgac
      121 gatgactttc gctggcgtac aaacatcgac atggaagaaa acaccgggat gctcgagtcg
      181 tttgtgaact tcaaataccg agtggtcaaa aacgtgaata ttgtggccgg cttgcattac
      241 acaaatttcc tgctgaataa ttcgcaagcc cttgaacccc gggcagcatt aaaatgggac
      301 atcaccccga aaagcagcct gaatttcggg tatggcaagc atagtatgcc agaaaatatt
      361 gttacctatt ttgccaatgt ttacgatgat aatatggtag catcgacacc gaataagaat
      421 ctgggtttaa cgaaagcaaa ccacttcatt gttggtttcg accaacgctt aacgaaaaat
      481 atgaaagcca agatcgaact gtactatcag ggcctgtacg atatgccggt agaaaacctc
      541 gacaccagca atttttcaat agtagatccc tattcaaaca acatcatccc actggtaaat
      601 gaaggggaag gccgcaacta cggagctgaa tttaccttag agcgtttctt cgacaacagc
      661 tattattttt tagttacagg ttcgttatac aaaactgaat ataagacact taccaacgtg
      721 tggcacaata ccgtgttcga cggaacgtat gcatttaatt ttctggcagg taaagaattt
      781 attttgggga aaccagaaaa aggaaacacg ctcaacctga atacccgcgt ggtatacaac
      841 ggaggccgaa gaaacttagc agttaatgtt gaagaaagcc gaaaaaatgg atatgctgtt
      901 tacgattact caaatcctta tgaagatcgt ttgaataata tttttcaggt aaattttaca
      961 gcaagtttaa agctgaaccg caaaaacgtg acccacgaat tactgctcga tttcttcaat
     1021 ataactaata atcagtcaaa aatacgagaa tactataata caatatccaa agatcgtgat
     1081 tatgaaaagc aacttggcat aatgccaaat gtgatgtacc gcatacactt ttaagtcata
     1141 caattttgaa taaatgatta atcgccagtc aaaaatgatt ggcggttttt cttttataac
     1201 tacgcttaac atgatccgac taaagagaaa ttaattttta tacagtgtca gaaaggtcac
     1261 aatctgagcg gggtgggaca tctacggcag gtgctaagta tacctatgct aaagaatgtc
     1321 taaaaaaaat tcaaaatctt ttgggggggt gccgaaggct tgttgtcata gagccaaatt
     1381 aagaaacagt aaaacaaatt ttaaatctca aaataccatg aaaacacaaa ttttagcttt
     1441 aatgcttctg ttattatccc tctctacagc gtttgcacaa aaagattcat tgaaaaagaa
     1501 cgaactggtg atgtactctt tcattgttaa ccggactccc gataatttca acattcccct
     1561 gataggtttt atcaatattg ccaacggaaa ccataaaagc gccgaagtgg gttttgtgaa
     1621 ttatacaaaa ggtaatttca ccggcggaca gattagcttc atcaacactg tgggagaaaa
     1681 ttgtattggt tcgcaaatcg gctttgtaaa taccaccgtg aaaaaattca aaggttctca
     1741 aatcggtttc gttaacacca cattaggcga cttcttcgga tcacaggttg gctttgtaaa
     1801 cacctcggca aaaaagtcag acaatattca aatcggtttc gtaaacacct gtggcgattc
     1861 actccggggc gcacaagttg ggtttgtaaa tacctcgctg aaacggactg ttgctccgca
     1921 aattggactt ataaatgttg caaaatcgct gaaaggtacg caaataggct tggtaaactt
     1981 tgtcgattct attgagtcgg gcgttcctct tggtttactt tcaattgtaa gaaaaggtgg
     2041 atatcatgcc atagaactgt cggttaacga aatgtatcca ctcaatttat cgtataaaat
     2101 tggtattccc gagttgtaca cctcgttcgt gatttcgtac aatccgaagg atgaaaaaaa
     2161 tgtagccatt ggtgccggcc tcggatcaat tatcccgttg ggaaaacgct tgtgcttcaa
     2221 tcctgaattc acgtcacaaa ccttgttctt caatgaattt caacaataca ccaacgctag
     2281 tgcaaatatt gggtataaac tgaacaatca tgtcatgata tctgccgggc catcgctcac
     2341 ttaccaatat aattacaaca agaattcgct aaatgaaccc ctctttactt tctcggaaag
     2401 caactggaga aatgggacct tcatcaccgg ggcaagggtt gccgttcgat ataaatttta
     2461 atctaaacac aagcttaagt atatttacag caggtgcatg gcggcaaaac caatatgtgc
     2521 ctgtttcatt taaaatctct taatcaaaaa gatggtgaaa gccaccaatt gtacaacaca
     2581 ttaaaattat tgaaatgaaa cgtacacttc ttacccgata ttttttagtt acatttttct
     2641 ggtcatggat aatctggata tcaaccgcac ttctttgtaa agcaggagta ataggcgacc
     2701 aaccggccga caaggttata aaaacagttg ttcttgtact cggcgctttt ggcccattgg
     2761 ttggggcact tgttgcggta agatcagaaa aaaacaaggg tggggttggg ctctatttaa
     2821 aacgttttct ttcctttaat atcgggtata aggcttttat tttcccatta cttattcttt
     2881 gcggtactac gctgcttgca tggtttttac catcgctgtt cggatatcaa aaaatctcaa
     2941 tgttgcttcc ttcggcatgg gtctttgtgc cctaccttat tgctatgatg ctctttggtg
     3001 gcggtcagga agaatttgga tggaggggtt atgctcttcc aaaacttgaa gaaaacctgg
     3061 gatactggaa gtcaaatatc ctgttgggaa ctatttgggc cgtgtggcat ttgccccttt
     3121 ggttcattcc gggaacaagt cagcagttaa tgcatttcgt tggttttatg atgcttacta
     3181 ttggttattc gtttctcttc tcgtggttta gaaaactggc tggcgaaaaa ccattttcag
     3241 gcatatacgt tcatggattg gcgaacgcgt ttattccgct atttccgatc ttgaacatgc
     3301 aacccggcgc accgcaacca gcattt
//
LOCUS       DAOPUW010000143         3319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_29391_length_3319_cov_3.331495, whole genome shotgun sequence.
ACCESSION   DAOPUW010000143 DAOPUW010000000
VERSION     DAOPUW010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29391_length_3319_cov_3.331495"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PKJ43_03450"
     CDS             complement(<1..309)
                     /locus_tag="PKJ43_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ71647.1"
                     /translation="MKNFALLGAAGFVAERHLRAIKETNNSLVVACDMFDVMGRMDSY
                     FPEAEFFADFDSFTAFIQKQQLSSSSKIDYCTVCTPNYLHFPHIKFALEHGMNVICEK
                     P"
     gene            complement(342..899)
                     /locus_tag="PKJ43_03455"
     CDS             complement(342..899)
                     /locus_tag="PKJ43_03455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNZ71648.1"
                     /translation="METYIHETACVDQGAIIGKGTKIWHFCHVMSGTNVGENCILGQN
                     VFIGSKAIIGNNVKIQNNVSVYDAVFIEDDVFCGPSCVFTNVINPRAFVERKTEYRPT
                     RVKKGATIGANATVLCGVTIGEFAMIGAGAVVTKDVKPFSLMTGVPAKRSGWVSRNGH
                     VLGNDLVCPETGERYIEEKGELKLI"
     gene            complement(978..2072)
                     /locus_tag="PKJ43_03460"
     CDS             complement(978..2072)
                     /locus_tag="PKJ43_03460"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNZ71649.1"
                     /translation="MQFCDLNRQYEAYRDEIDEAVRSVISSSSFINGKEIELLETGLA
                     QYCGAKHAIVCSSGTDALLLAMMAYGLQPGDEVICPAFSFFASASMVSFLNGIPVFVD
                     VSPTNFTIDVDKIEEKITLRTKGIIAVSLFGQCPDFEAINELAEKYGLWVIEDGAQSF
                     GATYFGQKSCTLTEVSTTSFFPAKPLGCYGDGGAVFTSNDELAHLIKQLRSHGQAQRY
                     YHQRIGLNARMDTLQAAILRVKLKHFDQELIIRQTAAEAYTKLLSDWVMTPLVNDGCI
                     SSWAQYTIIVKDREKLRVDLASKNIPTAVHYPIPLPAQEAFSHLAHPDEVFKVAGLLS
                     DTVLSLPMHGLITLAEVETVAKAIIESLED"
     gene            2202..3107
                     /locus_tag="PKJ43_03465"
     CDS             2202..3107
                     /locus_tag="PKJ43_03465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ71650.1"
                     /translation="MTAISAIISTYNRAQFLDGLFDSILQQTVDCSRYEVVIVNNNCT
                     DNTEAICHKFMVEHPEISVNYCIETKQGLSHGRNRGIVESTHSIVTFLDDDAVIAPDF
                     FEKTMDFFEKHPAVNAIGGKILLQYMERKPDWYNPFLASILGYFNPGDKEEVFSGRNY
                     FRGSNMSFRKEVFNKYDGFNTTLGRVGKNLAGSEEKELFYRLKNKGEEMWYVPSTVVY
                     HLVPIERTYSEFVKRQAIGTGNSQRQHALIEGKISFIKCLASEGGKWVVSFLLAIFYI
                     FTFQFPVASMLLRFRWWVSRGIILK"
BASE COUNT         1030 a          699 c          636 g          954 t
ORIGIN      
        1 tggcttttcg caaatgacgt tcatgccatg ttcaagcgca aactttatat ggggaaaatg
       61 aagataattt ggggtgcaaa ccgtgcaata atcaatttta gatgaagaag acaattgctg
      121 tttctgaata aaggcagtga acgaatcaaa atcggcaaaa aactcagctt caggaaaata
      181 gctatccatt ctacccataa catcaaacat atcgcaagcc accacaagag aattgtttgt
      241 ttcttttata gccctcaaat gtcgttcggc tacgaaacct gccgcaccca ataaagcaaa
      301 attcttcatt atattattct atgtaaatca atagtttaaa actaaataag ttttagttct
      361 cctttttctt ctatatatct ctcacctgtt tcggggcaaa ctaagtcgtt acccaataca
      421 tgaccgtttc ggctaaccca acccgatctt ttcgccggca cacctgtcat aagtgaaaaa
      481 ggctttacat ctttggttac aacagcccca gcgccaatca tggcaaattc accaatggtg
      541 accccacata aaactgttgc attggcacca atagtagccc ctttctttac ccgggttgga
      601 cggtattcgg ttttcctttc gacaaacgcc ctgggattaa ttacattggt aaaaacacaa
      661 ctcggcccgc aaaatacatc atcttcaata aaaacagcat cgtaaacgct tacattattc
      721 tgaattttta cgttgttccc aataatggct ttacttccaa tgaaaacatt ttgccctaag
      781 atgcaatttt cgccaacgtt tgtcccagac ataacatggc aaaagtgcca tatttttgta
      841 ccttttccaa tgatagcgcc ctggtcgaca caagctgttt catgaatata cgtttccata
      901 ttgctatttt tcaatgtgtt tatacaatgt atcagacggc atttttattt aaaacaaccc
      961 ttgtagccat taatccgtca atcctccaaa ctttcaataa ttgccttagc caccgtttca
     1021 acctcggcga gagtaattaa accatgcatg ggcaacgaca aaacggtatc ggaaagcaac
     1081 ccggctactt taaatacttc atcgggatgc gcaagatgcg aaaaagcttc ctgagctggc
     1141 aacggtatag gataatgcac ggctgtgggg atattctttg aagccaggtc gaccctgagt
     1201 ttttcgcgat cttttacaat aattgtgtat tgggcccacg aactgatgca accatcgtta
     1261 accaaagggg tcataaccca gtcgcttagg agctttgtat atgcttcagc agcagtttgc
     1321 ctaatgataa gttcctgatc aaaatgcttc aactttaccc taaggatagc tgcctgcaag
     1381 gtatccatcc gtgcattaag tccaattcgc tgatggtaat aacgttgcgc ttgtccgtgc
     1441 gacctaagct gcttaatcaa atgagccagc tcgtcgttgg atgtaaaaac agccccccca
     1501 tcaccgtaac aaccaagcgg tttggccggg aaaaaacttg ttgtcgacac ttcggtaaga
     1561 gtacacgatt tctgcccaaa ataagttgca ccaaacgact gagccccatc ttctataacc
     1621 caaaggccgt atttttcggc caattcgtta atcgcttcaa aatcggggca ttgcccaaac
     1681 aacgaaacgg caataattcc tttggtccgg agggttattt tctcctcaat tttatcgaca
     1741 tcaatagtaa agttggtagg tgaaacatcg acaaatactg gaataccatt taaaaaactg
     1801 accattgaag ctgaggcaaa gaagctgaat gccggacaaa taacttcatc gcccggttgg
     1861 aggccatacg ccatcatagc tagcaacaaa gcgtcggttc cggaactaca cacaatggca
     1921 tgtttcgccc cgcaatattg ggctaaccct gtttcaagga gttcaatttc tttcccattt
     1981 ataaaggaag atgagctaat taccgagcgt acagcctcat ctatttcatc cctgtaggct
     2041 tcgtattgac gattaaggtc acaaaattgc atgataaaaa ctaaatgata ttctgcaaaa
     2101 ataacggaaa aggggcgaga atatgattta tatttaggaa aatgcttttt tgacgattgc
     2161 gaaatcgtaa ttttgtcaac caaacaagaa atcacatagg aatgacagca atctcagcca
     2221 taatttcgac atataaccgg gctcaatttc ttgacggttt attcgattcg atattgcagc
     2281 aaacggttga ttgcagtcgt tacgaagtag ttattgtcaa taataattgc accgacaata
     2341 ccgaagcaat ttgccacaag ttcatggtcg aacatcctga aataagcgtt aattactgca
     2401 ttgaaactaa acaaggatta tcgcatggac gtaaccgtgg cattgttgaa tcgactcatt
     2461 ccattgtcac tttcctcgac gacgatgctg tgattgctcc cgactttttt gaaaaaacca
     2521 tggatttttt tgagaagcac cctgcagtta atgcaattgg aggaaaaatt cttcttcaat
     2581 acatggaaag aaagcccgat tggtacaacc cttttttggc atcgatttta ggctatttca
     2641 atccaggcga taaagaagag gttttttcag gaagaaatta cttcagggga tctaacatgt
     2701 cgttcaggaa agaagtcttt aataaatacg acggctttaa tactactttg gggagagttg
     2761 gcaaaaacct cgcaggcagc gaagagaaag aactgtttta ccgtttaaaa aacaaagggg
     2821 aagaaatgtg gtatgtgcca tcaacggttg tttaccacct tgttccaatt gagcggacat
     2881 attccgagtt tgtaaagcga caagctatag ggacaggcaa cagccagcgg caacacgcac
     2941 tgatagaggg gaaaatatca tttattaaat gccttgcttc agagggaggt aaatgggttg
     3001 ttagtttttt attagctatt ttttatatat tcacctttca atttcccgtt gccagcatgt
     3061 tgctgcgttt tcgttggtgg gtgagcaggg ggataatact taaataagtc atgaaaagaa
     3121 cgattgtacg cctaataaca atgattctat cattttcttt aaccaatttt aaggctatta
     3181 atatttacaa aaaagggaat aagagtatca aaaatccttc ttgcgtttga ttgatttagt
     3241 ttacaaaaat gagaataatt atatttccag tcgggttaac gaaattctta agttcaatga
     3301 acaatcagta gactttata
//
LOCUS       DAOPUW010000144         3291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_29705_length_3291_cov_3.565513, whole genome shotgun sequence.
ACCESSION   DAOPUW010000144 DAOPUW010000000
VERSION     DAOPUW010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29705_length_3291_cov_3.565513"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..1621
                     /locus_tag="PKJ43_03470"
     CDS             257..1621
                     /locus_tag="PKJ43_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNZ71651.1"
                     /translation="MKNANILIVDDNKSILSALELLLKGRCAKVKTLSTPNLLLSELK
                     SEAYDVLLLDMNFSAGINTGNEGIYWLQRVLDQEPGLSVVMITAFGDVELAVKAVRMG
                     SVDFVLKPWENEKMLATIESAYKLNQSRKEVKKLKLKEKQLIAEINRPVQDLIGSSGA
                     WKQVIESVAKVAATNANVLITGENGTGKELIAREIHRLSPRSDKVMVTVDMGSITETL
                     FESELFGHKKGAFTDAHSDRTGKIETAHEGTLFLDEIGNLPLPMQAKLLSVLQNRAIT
                     RVGENIPVPVDVRLICATNCNLLQMVANGRFREDLLYRINTIHIELPPLRNRRADIAE
                     LANFFLKKYVEKYNKPSLSITEAALQKLETYAWPGNVRELQHAIEKAVILSDSAMLQP
                     SDFLFRSSDSVSDIQSNCTLEEMEQKLIAEAISKCEGNMTSVANQLGITRQTLYNKIK
                     RYGL"
     gene            1611..2957
                     /locus_tag="PKJ43_03475"
     CDS             1611..2957
                     /locus_tag="PKJ43_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ71652.1"
                     /translation="MAFKHYNRLLVIRLVVIVGLAFAIGCLVMMGKSLFFIIFLSSVE
                     VILVAEFVNFLNSTNRQISFFIQAINNDDTTLRFPMKTGNAIINDLHNSLNELNVILQ
                     KAKVSNQIRERYFSEILQHIGTGVLVLNEKGFITEVNPAALDLFGIQNLTHITQLDRV
                     DPKFKNELQLLAGSNKQVITLRKGNDAIQIVTRCSVINLKEEDIKLITLQDIRGELER
                     KELDSWVKLIRVLSHEIMNSLAPVTSIAQSLNSIWKEKLEAQPEYASDDAIESTMKGL
                     EVIGERGEGLIRFVQSYRMLTKVPVPQFSKVSVQNLFDRLSILVSPLKAEYRVAIQFY
                     APDSDFEVQIDEQMMVQVVINLVKNSVEALQGCENPKIEIHAQMLSGEKVEITVIDNG
                     PGVPEEILEEIFVPFFTTKATGTGIGLSHSRQIMRAHGGSIGCNSEPGKTVFRLVW"
     gene            complement(3011..>3291)
                     /locus_tag="PKJ43_03480"
     CDS             complement(3011..>3291)
                     /locus_tag="PKJ43_03480"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNZ71653.1"
                     /translation="AIRRINGATLGNIIWAFVLDLAYIALPAILIGLVFAWITATKWM
                     QNFESKITLHWALFALCCLFILVSIAIIAAVNYARTARHNPVESLRYE"
BASE COUNT          988 a          660 c          755 g          888 t
ORIGIN      
        1 tcaattttca atttccccac tcatagccaa ggatgtgcca aaattgcaat aatcattaaa
       61 tgagagatat atgaagatag ggggatttta tgtgtaaata aattagacaa tcactgtaaa
      121 aatcttttac aaaccactaa ctttcggaaa aatgtatatg gttgtcaatc agtcaacaaa
      181 gaatagggtt taatatttgt aaattttatt cgtgtactta gtgaatgaac tttgtgtact
      241 tagtgtttag tgaaatatga aaaacgcaaa tatcttaatt gtcgacgata acaagagtat
      301 cctcagtgcg ttggaactct tacttaaagg gcgttgtgcc aaggtgaaaa ccttgtcgac
      361 acctaacctg cttttaagcg aactgaaaag tgaggcctac gacgtgcttt tgctcgatat
      421 gaatttttcg gcagggatta ataccgggaa tgaagggatt tactggctgc aacgggttct
      481 ggatcaagaa cctggactta gtgtggtaat gattacagcc tttggcgatg tggaattggc
      541 agtgaaagct gtacgcatgg gttccgtcga ttttgtgctc aaaccatggg agaacgagaa
      601 aatgctcgcg acgatcgaat cggcttataa actaaaccag tcgcgcaaag aggtgaaaaa
      661 gctgaaactt aaagaaaagc aattaattgc cgaaatcaac cgtccggtgc aagacctaat
      721 tggttcttcg ggcgcatgga aacaggttat agagtcggtg gccaaggtgg ctgctaccaa
      781 tgccaatgta ctgattacag gcgagaatgg aacgggcaag gagttaattg cccgcgagat
      841 acaccgactt tcaccacgct ccgacaaggt gatggtaacc gttgacatgg ggtccataac
      901 cgaaaccttg ttcgagagcg aactctttgg tcataaaaag ggtgcattta ctgatgccca
      961 ttccgaccgt acaggtaaaa tagaaacagc acacgaaggt acgctattcc tcgatgaaat
     1021 tgggaactta ccgcttccga tgcaggccaa actgctgtcg gtattacaaa atcgtgccat
     1081 tacgcgtgtt ggcgaaaata ttccagttcc agttgatgtc cggctcatct gcgctaccaa
     1141 ctgcaacctg ctgcaaatgg tcgccaatgg ccgcttccgc gaggacttgc tttaccgtat
     1201 caataccatt cacattgaat tgcctccgct gcgcaaccgc cgtgccgata ttgctgaatt
     1261 ggccaatttc ttcctgaaaa aatatgttga aaaatacaac aagccaagct taagcattac
     1321 ggaagcagcc ttacaaaaac tcgaaactta tgcgtggcca ggtaatgtgc gcgagctgca
     1381 acatgccatt gaaaaggctg tgattttaag tgattcagca atgctgcaac cgtccgattt
     1441 cctgtttcgc agttccgatt ctgtttctga tattcaatcg aattgtacgc tcgaagaaat
     1501 ggaacagaag ttaatagccg aggctatttc aaagtgtgaa gggaatatga cttcggtagc
     1561 caatcaattg gggattactc gtcaaacatt gtacaataaa attaaacgtt atggccttta
     1621 agcactacaa ccgtttgctc gtaatccgtt tggtggtgat tgttggattg gcctttgcaa
     1681 ttgggtgttt ggtgatgatg ggtaaatctc tgtttttcat cattttttta agttcggttg
     1741 aagtgatttt ggtagccgaa ttcgttaatt ttttgaattc tacaaaccgt caaatcagtt
     1801 ttttcattca agcaattaat aacgacgata ccaccttgcg gttcccaatg aaaaccggaa
     1861 atgccattat taatgatttg cacaacagcc taaacgaact gaacgtgatt ttgcaaaagg
     1921 ccaaggtgag caatcaaatt cgcgagcgct atttcagcga aattttgcaa cacatcggaa
     1981 cgggtgtgtt ggttctcaac gaaaaaggat ttattacaga ggtgaacccg gctgcactcg
     2041 atttatttgg aattcaaaat ctcacacaca tcacccagct tgatcgggtc gatccgaaat
     2101 tcaaaaatga acttcaactc ttggccggat cgaataagca agttattacc ctgcgaaagg
     2161 gcaacgacgc cattcaaatt gttacgcgct gttcggtaat caacttgaaa gaagaagaca
     2221 ttaaactcat aacccttcag gatattcgag gcgaattgga gcgaaaagaa ctcgattcgt
     2281 gggtgaaatt gatacgtgtt ttgagtcacg aaattatgaa ttcattggcg cccgtaactt
     2341 ctattgcaca gtcacttaat tcaatatgga aagagaaact tgaagcacag cctgaatatg
     2401 caagcgacga cgccattgaa agtaccatga aaggccttga agtgattggt gaaaggggcg
     2461 agggattgat ccgctttgtg cagagctatc gtatgctgac caaggtgccc gttccgcaat
     2521 tttcgaaggt aagcgttcag aatctgttcg atcgactgag cattttggta tcgcctttga
     2581 aagctgaata cagagttgcc attcagtttt atgccccaga ttccgacttt gaggttcaaa
     2641 tagacgagca aatgatggtt caggtggtca tcaatttggt gaaaaacagt gtcgaggcat
     2701 tgcaaggttg tgaaaacccc aaaatcgaaa ttcatgcgca gatgctttca ggcgagaaag
     2761 tggaaattac cgtaatcgac aatggaccgg gtgtacccga agaaatttta gaagaaatat
     2821 ttgttccctt tttcaccaca aaagccaccg gtactggaat tgggctcagt cattcgcgcc
     2881 aaattatgcg ggcacatggt ggcagtatcg ggtgcaattc tgaacctgga aaaacagtgt
     2941 ttcggctggt ttggtaaaaa caggtaaaga gtttatatta aaaacccttt atacgttgaa
     3001 tcgttcatat ttattcgtac cgcagacttt ctaccgggtt gtgacgtgcc gtgcgagcat
     3061 agttcactgc tgcaataatg gcaatcgata caaggatgaa caagcaacat agtgcaaata
     3121 aagcccagtg aagcgttatc tttgattcaa agttttgcat ccatttggtg gccgtaatcc
     3181 atgcaaatac taatccgatg agaatagccg ggagggcaat gtaagccaaa tcgagcacaa
     3241 aagcccaaat aatgttgccc agtgtggcgc cattgatcct cctgattgcc a
//
LOCUS       DAOPUW010000145         3289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_29729_length_3289_cov_4.454855, whole genome shotgun sequence.
ACCESSION   DAOPUW010000145 DAOPUW010000000
VERSION     DAOPUW010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29729_length_3289_cov_4.454855"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="PKJ43_03485"
     CDS             complement(<1..884)
                     /locus_tag="PKJ43_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNZ71654.1"
                     /translation="MAKEKKFVTCDGNYAAAWMSYMFSEVACIYPITPSSTMAEYVDE
                     WAANGKTNIFGRPVRLAEMQSEGGAAGAVHGALQSGALTTTYTASQGLLLMIPNMYKI
                     AGELLPCVFHVSARALAAHALSIFGDHSDIYAARQTGFAFLAAGSVQEEMDLAGVSHL
                     ATLKSKVPFCAFFDGFRTSHELQKIEVITQEQMMPLIDMDLIQAFRDKALNPENPVER
                     GTAQNPDIYFQGREASNKFYNAVPEIVENYMSEITKITGREYHLFDYYGAEDATDIIV
                     AMGSVTETTKEVIDYLMA"
     gene            complement(955..2172)
                     /locus_tag="PKJ43_03490"
     CDS             complement(955..2172)
                     /locus_tag="PKJ43_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ71655.1"
                     /translation="MNYSLSLFFKIELMDFFWDAHKNMINSTKSVVRRELIDEIDWNE
                     RLIAIKGFRGVGKTSFLIDCIREKFPDPKESLYINLNSFYFTRRKISGFADEFYKRGG
                     KVLVLDQIHKYPGWAEELRECYDKLPGLSIIFSASPVLRVIEGNDYLKDIAKIYHLPG
                     LSFREFLNYRTNNNFKCYDFEEIIENHVEIANQIVSKVKPLAYFTDYLQYGYYPYFLD
                     RHSYFSDDLLKHINLALEIDVTYLNQIDLKYLPKLRKLLYIVAKEVPFSPNVSKLSNE
                     ITTSRATVMNYLRYLKNARLINLLYTNGDEDQQKKPTKIYLHNTNILHAVAPENNDNA
                     TNRQTFFYNQVSYRNDIKGVEGFDFKVNNNYCFTVGGKYAEPVNENSYAASDRIEIGE
                     DRKIPLWLFGFLY"
     gene            2647..3243
                     /locus_tag="PKJ43_03495"
     CDS             2647..3243
                     /locus_tag="PKJ43_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HNZ71656.1"
                     /translation="MDKRSFFKTSMVALGGLLIPRKMFALEYYPTTKNGEWAILYGTW
                     CGSTRDASIWISEGMNGIANVFDVRENPDLSKYKHIVIGGAIRAGVVPKELQTYLENN
                     KSVIEGKVRGLFAVCGNMKQPVNQEQHNRLINNHLAKLAGAGNVPSKVFLGRITFGLM
                     EPDVQKQMRSMMNNEEYDNLKRSECIDFGREVFKSINQ"
BASE COUNT          908 a          605 c          702 g         1074 t
ORIGIN      
        1 tgagccatca agtaatcgat tacttctttt gttgtttcgg ttacagaacc cattgcaacg
       61 atgatgtcgg tagcatcttc agctccgtaa taatcgaaca ggtggtattc gcgaccagta
      121 atcttagtga tttcgctcat gtagttttct acaatttcag gaactgcatt gtagaatttg
      181 ttcgaagctt ctcttccctg gaagtagatg tcggggtttt gagcagtacc gcgttctaca
      241 gggttttctg gattcaatgc tttatcacgg aatgcctgga taaggtccat gtcgatcaaa
      301 ggcatcattt gttcctgagt aattacttca attttctgta attcgtgtga agtacggaaa
      361 ccatcgaaga atgcacagaa aggcactttt gattttaatg tagcaaggtg ggaaacacct
      421 gccaggtcca tctcttcctg aacagaacca gctgcaagga atgcgaaacc agtctgacgt
      481 gcagcataga tatcgctatg gtcaccaaaa attgataatg catgagctgc caacgcgcgt
      541 gcacttacgt ggaaaacaca tggcaacaat tcgccggcaa ttttgtacat gttagggatc
      601 atcagcagca atccctgaga tgcagtgtaa gttgttgtta atgcaccact ttgcaatgca
      661 ccgtgcactg caccggctgc gccaccttca ctttgcattt cggccaggcg cactgggcga
      721 ccgaatatgt ttgtttttcc gttagcagcc cattcgtcaa catattctgc cattgtcgac
      781 gaaggagtga tcggatagat acaagccact tcgctgaaca tatagctcat ccacgctgct
      841 gcatagttcc cgtcacaggt aacaaacttt ttttcttttg ccataacttc ttcagtgtta
      901 ttaattattg ttatattttt ttctatctcg tttattttct ttcaattatt gatgttaata
      961 caaaaagcca aacagccaca gtggaatttt tctgtcttca ccaatttcga tacggtccga
     1021 ggctgcataa ctattttcgt tgactggttc ggcatatttc ccgcctactg taaaacaata
     1081 gttgttgttt actttaaaat cgaagccttc aactcctttt atatcattgc ggtaactaac
     1141 ctgattgtaa aagaatgttt gcctgttggt agcattgtcg ttgttttcag gagctactgc
     1201 gtgtaaaata ttggtgttgt gcagataaat cttggttggt tttttttgct gatcttcgtc
     1261 tccgtttgta taaagaaggt tgatcaaccg ggcgtttttc aggtaacgga gatagttcat
     1321 tacagtagcc cttgatgtgg ttatctcgtt gctgagtttg ctcacgttgg gcgagaatgg
     1381 cacttctttg gctacaatgt aaagtaactt tctaagcttg gggagatatt tcaggtcgat
     1441 ttggttaagg taagttacat cgatttcgag ggccaggttg atgtgtttca gcaggtcatc
     1501 ggaaaaataa ctgtggcggt caagaaaata agggtaatag ccatattgaa gataatcggt
     1561 aaagtaggcc aggggtttta cttttgatac aatttgattg gctatttcga cgtggttttc
     1621 aatgatttcc tcaaaatcgt agcatttaaa attgttgttt gtacgataat tcaggaactc
     1681 acggaacgaa agccccggga ggtgataaat ttttgcaata tctttcaagt aatcgttccc
     1741 ttcgatgacc ctcaaaaccg gcgaagcaga aaaaataatg cttagtccgg gcagtttgtc
     1801 gtaacattcg cgcagttctt ctgcccatcc cgggtatttg tgtatctggt cgagcacaag
     1861 cactttgccg ccgcgtttgt agaattcatc ggcaaagccc gatattttcc tgcgggtaaa
     1921 ataaaaactg ttaaggttga tgtaaaggct ttctttgggg tctggaaatt tctcacggat
     1981 gcaatcgatc aggaacgagg ttttaccaac accgcgaaat ccttttatgg caatgagccg
     2041 ttcgttccaa tcaatttcgt cgatgagttc ccttctgact acgctttttg tgctgtttat
     2101 catgttttta tgtgcatccc agaaaaaatc catgagctca attttgaaaa acaaagatag
     2161 tgaataattc atttttttgt aatgtacaga tggcaatcgt gtgtaaatag tgtaatttgg
     2221 aattgataaa aataaactag aaaagggaga catgatgtta ttggactctt tgctggcttt
     2281 ggtaatgaaa tttaggtttc cttattcgat actaattcga ccttcttatt tcggctaatt
     2341 ttacaaacac aaaatcactg tttaatgaac tgaaatcctc atggttattt tctgccttaa
     2401 accttttttt ctatttttag tcatattaat tttagcaaaa gatgtatttc tgttttgttt
     2461 ttcaattgat taattactag gcgtttaaaa aattaacggt cataaattgt cgttcatctc
     2521 tggattagaa attatttgaa cagtattttt actgcgaata tgcttatggt tacattgtta
     2581 tttgccaggc agatgcatct tttgtatgag tgaaaattgc tttatcaacc ttgaaaacta
     2641 attccgatgg ataaaagaag ttttttcaaa accagtatgg tggccttggg tggcttgcta
     2701 atcccacgta aaatgttcgc gctggaatac tatccaacaa caaaaaatgg agaatgggcc
     2761 attttgtatg gtacatggtg tggttctact cgtgatgcaa gtatttggat atcagaagga
     2821 atgaatggaa tcgccaatgt ttttgatgtg cgcgaaaatc ccgatttatc gaagtataaa
     2881 cacattgtta taggaggggc tattagagca ggagtcgttc ctaaagaact tcagacatat
     2941 ctcgaaaaca ataaatcggt cattgaaggc aaggttaggg gcttatttgc tgtttgcgga
     3001 aacatgaagc aacctgtgaa tcaggagcaa cacaacaggc ttataaacaa tcatctggca
     3061 aagcttgctg gcgcaggaaa tgtgccttcg aaggtttttc tggggcgcat cacctttggg
     3121 cttatggagc ccgatgttca gaaacaaatg cgttcgatga tgaataatga agaatacgat
     3181 aatcttaaac gaagcgaatg tatcgacttt ggtcgtgaag tttttaaatc tataaaccaa
     3241 taataatgaa gaagccgatt acttttctgt cagtattttt tcttgttgt
//
LOCUS       DAOPUW010000146         3281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_29839_length_3281_cov_3.278983, whole genome shotgun sequence.
ACCESSION   DAOPUW010000146 DAOPUW010000000
VERSION     DAOPUW010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29839_length_3281_cov_3.278983"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="PKJ43_03500"
     CDS             <1..1088
                     /locus_tag="PKJ43_03500"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNZ71657.1"
                     /translation="KQNKGTMKRSWAITLIIIGVAATASIFLYLAYNNEKPVVLPEER
                     QGVAIIMTGAAARIPQEAALLEELDKRGMLDDIVFISGVSSGALNAVLLNGIIDKKIT
                     WKEYRNILYSIKNKDIVVYDHVQFPFDTSPARALYTKIVEKKLGYKTIGDLPVTTSIS
                     VAYIKKLPLKKVAYRMCSRKINEESDTTLNLVDILMASTAFPIVFPPVKIPNAKTIPD
                     VEYIDGGVGDDEIPFKALLQFEKKRGIGVEKVFIISRNGDDATKISEEIDALGIDDDG
                     VFDELELTFDTFLKRGLIKRLKAYAVEAPELVPLTYVWIPDFKENFPMFDFGTMKQQY
                     NVTTRWAKKNPPTPFAEYLLPYLLEEK"
     gene            complement(1095..1889)
                     /locus_tag="PKJ43_03505"
     CDS             complement(1095..1889)
                     /locus_tag="PKJ43_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71658.1"
                     /translation="MLNRFFIPFLLVASIFWSCEDSGEIFTPNYPTTIQTVSSEEIDL
                     IFSELYETPLYECTSVDDYGFCHVTTSSTACGKFDSIYVNYSYEELENIFHDALIEHS
                     ELLNISDLSGITISSIKNLKGMTFEAFKKAYPDSATTGWVITSNVQRVKNYPIYGTEI
                     KVMVFFDRVRAIGGRRFSNLNIPDSDVYSESEAKEKFIDQEISSGSTILKITDDSFWY
                     TSEKVIFPYQTNNKIELRLCWKLRPEKWTVFVDTQTGRSFGVIKPN"
     gene            2139..3263
                     /locus_tag="PKJ43_03510"
     CDS             2139..3263
                     /locus_tag="PKJ43_03510"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNZ71659.1"
                     /translation="MLTNIYRKEIEELFKTPIVQQTAFWSIVKKRLGANTLAINFKAQ
                     KSGMYASSNSSELIISDLLLIIRKIDKIHSVAYIPYGPELEPDEEFQGLFLEELSECL
                     RSFLPKNCIMIRYDLCWESYWAKDEKIQGTDNIWAGTPETRTQELRFNFNTLHWNFKK
                     TQFNILPSNTIYLNLSNDTETILQRMKPKTRYNIGLARRKGVTVRPIGMESLEIWYKL
                     YQETAQRNKIRINDINNFKAVLEAKTHDSQSPAEVILLLAELDHKPLAAMFLIITGNR
                     GSYLYGASSSENRNYMAPYALQWEAIKISKSRGCTEYDMFGVSPTPDPTHPLYGLYKF
                     KIGFGGDLFHSMGCWDYPLDQDKYNCFIASEMNSFGYHVN"
BASE COUNT         1130 a          656 c          595 g          900 t
ORIGIN      
        1 aaaaacaaaa caagggaacg atgaaaagat catgggctat aacattgata attataggag
       61 ttgcagcaac agcatcaatc tttctatacc ttgcatacaa taacgaaaaa cccgttgtcc
      121 tgcccgaaga acgacaagga gttgccataa ttatgaccgg tgctgcggct cgaattcctc
      181 aagaagctgc cttgctcgaa gaactcgata aacgcggcat gctagatgac atcgtgttta
      241 tatcaggagt tagctcagga gcgttaaatg cagtgttact caatggaata atcgataaaa
      301 aaataacatg gaaagaatat cggaatatct tgtacagcat aaagaataaa gacattgttg
      361 tgtacgacca tgttcaattc ccatttgaca cttctccagc gcgtgcactt tacacaaaaa
      421 ttgtagagaa aaaactggga tacaaaacca taggcgacct accggtgacc acatcgatat
      481 ctgttgcata cattaagaaa cttccattaa agaaagtcgc ttaccgaatg tgcagcagaa
      541 aaataaatga agagagcgat actaccctca atttagtaga tatactaatg gcctctacgg
      601 catttcccat cgtgtttcct ccagttaaga ttcccaatgc gaaaactatt cccgatgtgg
      661 aatatatcga tggtggagtt ggcgatgatg aaattccatt taaagcatta ctgcaatttg
      721 agaaaaaaag gggcattgga gttgaaaaag tgttcattat cagcagaaac ggagatgatg
      781 ccacaaaaat tagtgaagaa attgacgcgt tgggaataga cgacgatgga gtattcgacg
      841 aacttgaact caccttcgac acctttctaa aaagagggtt aattaaaagg ttaaaagcgt
      901 atgccgttga agctccggaa cttgtgcctc ttacgtacgt ttggattccc gattttaaag
      961 agaatttccc aatgtttgat tttggtacca tgaaacaaca atacaatgta accacacgat
     1021 gggcaaagaa aaaccctcct accccgtttg cagagtatct tttaccttat ttacttgagg
     1081 aaaagtaatt acaattagtt tggtttaatc accccaaaac ttcggcctgt ctgagtatca
     1141 acaaatacag tccatttctc aggacggagc ttccaacaga ggcgcagttc tattttattg
     1201 ttggtttggt atggaaagat cactttctcg ctagtatacc agaacgaatc atcggtgatt
     1261 ttaaggatcg ttgaacccga gctaatctct tggtcaataa acttttcctt tgcctcgctt
     1321 tcagaataca catcactgtc aggaatattc aaattagaaa agcgcctacc cccaatcgca
     1381 cgtacacgat cgaaaaaaac catgacttta atttcggtcc catatattgg atagttttta
     1441 acccgctgaa catttgaagt tataacccaa cccgtggtag cagaatcggg ataagccttt
     1501 ttgaaagctt caaacgtcat ccctttcaaa tttttgatcg aagatatagt gataccagat
     1561 aggtcactta tattcagcaa ttcggaatgt tcaataagcg catcgtgaaa aatattttcc
     1621 aattcttcat acgaataatt aacataaatc gaatcaaatt taccgcaagc cgtggaagaa
     1681 gtagtcacat gacaaaaccc gtaatcatct accgaagtgc attcataaag cggagtttca
     1741 tataattcac taaaaattaa atcaatttct tcagaagaaa ctgtctgaat tgttgtggga
     1801 taattgggtg taaagatttc gccactgtcc tcgcacgacc aaaatattga agctaccaac
     1861 aaaaatggta taaaaaacct attcaacatg gattcttttt ttcacgaaga taaccatggc
     1921 tactgataat ttgaaaaata ggtcaacgaa aatagaatga attatacttt tttatcattt
     1981 aaacaaaaca atcttcaaca ataagacgca aacgagaagt gcttcatctt caataaatcg
     2041 gcataatttt caaatactaa attgctccaa attaaatgct aagttttttt aatagaatgt
     2101 attagctacc tttgcaggac aacaatttat tttcagagat gttaactaat atttaccgta
     2161 aagaaataga agaactcttc aaaaccccaa ttgtacaaca aaccgccttc tggtcgatag
     2221 taaaaaagag actgggagcc aacacgctgg caataaactt caaggctcaa aaatcaggaa
     2281 tgtacgcaag ctccaatagt tctgaactca taatctcaga tttgctgctt attattcgaa
     2341 aaatcgacaa aatccactca gtggcatata ttccatatgg ccctgaatta gagcccgatg
     2401 aagaatttca aggactattt ctcgaagaat tatctgaatg tttacgttcg tttttaccta
     2461 aaaattgtat aatgatacgt tacgatcttt gctgggaatc ttattgggca aaagacgaaa
     2521 agatacaagg aacagacaac atttgggctg gaacccccga aacccgcact caggaactta
     2581 ggttcaattt caatacgtta cactggaatt tcaaaaaaac gcaattcaat attttaccat
     2641 ctaacaccat ttatcttaat ctttccaacg acactgagac cattcttcag cgaatgaagc
     2701 ccaaaacaag atataacatt gggcttgcca ggcgcaaagg agttactgtt aggccaatag
     2761 ggatggaaag tttagaaatc tggtataaac tttatcagga aacagcacaa cgaaataaaa
     2821 taagaatcaa cgatataaat aattttaaag cggtactcga ggccaaaacc cacgattcgc
     2881 aatcgccagc agaagttatt ttgttgcttg ccgaactcga tcacaaacca ctggctgcaa
     2941 tgtttctgat aattaccgga aaccgtggtt cttatcttta tggagcctca tcttcagaaa
     3001 accgcaacta catggctccc tacgctttac agtgggaagc aattaaaata tcgaaaagcc
     3061 ggggctgtac agaatatgac atgttcggag tttcaccaac ccccgaccct acccatcctc
     3121 tttatggact ttacaaattt aagattggtt tcggcggaga cttattccac agtatgggat
     3181 gttgggatta tcctctcgat caggataaat ataattgttt cattgccagc gaaatgaata
     3241 gttttggtta ccatgtcaac taatcgataa caaaaccaag t
//
LOCUS       DAOPUW010000147         3269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_30006_length_3269_cov_3.305849, whole genome shotgun sequence.
ACCESSION   DAOPUW010000147 DAOPUW010000000
VERSION     DAOPUW010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30006_length_3269_cov_3.305849"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PKJ43_03515"
     CDS             <1..272
                     /locus_tag="PKJ43_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HNZ71660.1"
                     /translation="VYQLSISSTKSMTGHLLGAAGAAEAIATILAIKNNIVPPTINHF
                     ETDPELDPELDFTFNVAKKKEVNYALSNTFGFGGHNASVVFKKFK"
     gene            269..1000
                     /gene="rnc"
                     /locus_tag="PKJ43_03520"
     CDS             269..1000
                     /gene="rnc"
                     /locus_tag="PKJ43_03520"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNZ71661.1"
                     /translation="MIRTIFQRIKLFSSPRKEFYLFLKDILGFYPGNLKLYDIAFVHK
                     SASMTDSMGNLINNERLEYLGDAILGAVVAEFLYRRFPQRGEGFLTQMRSKLVNRAFL
                     TQLINETGMHRFIKSRTVSPLTACNIYGDALEALIGAIYLDKGYQKARYVIVKRFLDG
                     YVDLNKMEEQDNNYKSQLIEWGQKNKMEVTFQTDEEDSPNSKFSIFVTTVEIDNQLKG
                     KGYGSSKKESQQNAAREALSTIEPD"
     gene            1176..2738
                     /locus_tag="PKJ43_03525"
     CDS             1176..2738
                     /locus_tag="PKJ43_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ71662.1"
                     /translation="MDKRRIYILIIIMSVVVSLLIFVQMNIIKNAAHIREEQFGQIVQ
                     RCLMHVALRLEEEEFKQFILDDHLAQSAKAYSSQGKSKSSANESSPKQISFSLNVTQD
                     PSGKVVAQASVNESTPESDVMENNPVQNPDVFDELAELTDMEQRRFFQSFNNRDAFNR
                     NVQYQLMLANKPIEQRVDPALLSRFIEEEFTMNGIDLHYEYVVKSYNQGTEKIIFSSK
                     NYKATKKKEHKTPLFINDLGAQKPNYLKVYFPNSQKRFLGDVSYMVYPTFILLSFIIG
                     IFAYTIAIIFKQKKLSTIKNDFINNMTHELKTPISTISLAAQMLRDNSVNNSTSAIER
                     ISGVIFDESKRLGMQVEKVLQMAVFNEGRLKFKFKDLDIHELISTVVQNFELRVQSLN
                     GKITFLPGATSTFIYGDYVHITNVLFNLLDNAVKYCKGNPEIEVITETKKDKLIIYIK
                     DNGIGIAKEHQKQIFERFYRVPTGNVHDVKGFGLGLHYVHKIITAHNGTIEVESAINK
                     GTTFSIKFPISE"
     gene            2742..>3269
                     /locus_tag="PKJ43_03530"
     CDS             2742..>3269
                     /locus_tag="PKJ43_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016268617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ71663.1"
                     /translation="MDTKIKILLAEDDENLGLLLKEYLIAKGYDTDLFPDGETAYHGF
                     MRNQYNICLLDVMMPKKDGFTLARDIRLVNADIPVIFLTAKNLKEDVIEGFKIGADDY
                     MTKPFSMEELIFRIEAILRRISNDNQPSDQNVYHIGLYSFDVRKQILSHDGEEVKLTT
                     KESELLRLLVANANKV"
BASE COUNT         1050 a          599 c          665 g          955 t
ORIGIN      
        1 atgtttacca attgagcatc agttctacca aatcaatgac cggccactta cttggtgcag
       61 ctggtgcagc tgaagcaatt gctacaatac ttgcaatcaa gaacaacatc gtgcctccaa
      121 caattaacca ctttgaaaca gatccagaat tggatccaga attggatttt acctttaatg
      181 tggctaaaaa gaaagaagtt aattacgcct taagcaacac gtttggtttt ggtgggcaca
      241 atgcatctgt ggtttttaag aaattcaaat gataagaacc atttttcaac ggataaaact
      301 cttttcttct ccgaggaaag agttttattt atttttaaaa gatattctcg ggttttatcc
      361 tggtaattta aagctttacg atattgcttt tgtccataaa tcggcttcaa tgaccgattc
      421 aatgggaaac ctgatcaaca atgagaggct cgagtatctt ggcgatgcca ttttgggcgc
      481 tgtggtagcc gaatttctct atcggcgctt tccccagcgt ggagaaggct tccttaccca
      541 aatgcggtct aaacttgtta acagggcttt cctcacccag cttattaatg aaacgggtat
      601 gcaccgtttt atcaaatcac ggacagtaag ccctctcact gcttgtaaca tttatggtga
      661 tgccctggag gctctcatag gtgccattta tctcgataaa gggtaccaga aagcccgtta
      721 tgttattgtg aaacggtttc ttgatggtta tgtcgatttg aataaaatgg aagagcaaga
      781 caataactat aagagccagc ttatcgaatg ggggcagaaa aataaaatgg aagtcacttt
      841 tcagactgac gaggaggatt caccaaacag taaattcagc atttttgtta ctacggttga
      901 aattgacaac cagttgaagg gaaaggggta tggcagttct aagaaggaat cgcagcaaaa
      961 tgcagcccgt gaagctctca gcacaatcga acctgattaa taaatattct cttatttttt
     1021 aatttgaaat tcaatctgtt gtgaggtaaa taggttgaat gctcaatcat gcttgttgtt
     1081 gtacaatctg gttcatcgtt aaaaaaggtt tacagccggt taaataatgt taagttgaaa
     1141 atccgggcca ttgtaaatca gttatctttg ttcctatgga taaacgacgt atctacatcc
     1201 ttattattat aatgtcggta gtagtgagtt tattgatatt tgtgcaaatg aatatcatta
     1261 aaaatgctgc ccacatcagg gaagagcaat ttggacaaat tgttcagcgt tgccttatgc
     1321 atgtcgccct gcgccttgaa gaagaagagt tcaagcagtt tattctcgac gatcatttgg
     1381 cacaatcggc aaaggcatat tctagccagg gtaaatccaa atcatcagca aacgaaagca
     1441 gtccaaagca aattagtttc tcgttaaatg ttactcagga tcccagtggg aaagttgtag
     1501 cacaggcatc tgtcaatgaa agtactccag agtcagatgt catggaaaat aatccggttc
     1561 aaaaccctga tgttttcgat gagctggcag aattgactga tatggaacag cggcgttttt
     1621 tccagagttt taacaatcgt gatgcgttca accggaatgt tcagtatcag cttatgctgg
     1681 ccaacaaacc aatagaacaa cgtgtcgatc cagcgttgtt gagccgtttt attgaggaag
     1741 aatttacaat gaatggcatt gaccttcatt acgagtatgt ggtaaaatca tataatcaag
     1801 gcacggaaaa aataattttc agttcgaaga actataaggc aactaaaaag aaagagcata
     1861 aaacaccgct gtttatcaat gatttagggg cacagaagcc caactatctg aaagtgtatt
     1921 ttccgaatag tcaaaaacgt tttcttggcg atgtaagcta catggtttat cctactttta
     1981 ttttgctttc gttcattatc ggcatttttg cttacacaat agctattatt ttcaagcaga
     2041 aaaaattgtc gaccataaag aacgatttta tcaataacat gacccatgaa cttaagaccc
     2101 caatttcaac catttcgctg gcagcacaaa tgctccgcga caattcggtg aacaattcaa
     2161 catcggccat tgaacgaatt tcaggagtta ttttcgacga aagcaagcga ttgggcatgc
     2221 aagtagagaa agtgttacag atggctgttt ttaatgaagg tcgtttgaag tttaaattca
     2281 aagatttaga tattcatgag ctgatttcaa ccgtagtgca aaacttcgag cttagggttc
     2341 aatcactcaa cgggaaaatt acatttttac caggtgcaac ctctactttt atttatggag
     2401 attatgtgca catcacaaat gttttgttta acttgctcga caatgcagtg aaatattgca
     2461 agggaaatcc ggaaattgaa gtgattaccg aaacaaaaaa agataaactg attatttata
     2521 ttaaagataa cggtattggc attgctaaag aacaccaaaa acagatattc gaacgtttct
     2581 atagggtacc caccggaaat gttcacgatg tgaaagggtt cggcttaggt ttgcattatg
     2641 tgcataaaat aattactgcc cataatggaa caattgaagt tgaaagtgcg ataaataaag
     2701 ggacaacgtt tagtattaaa tttcctatat cagaataaac catggacaca aaaattaaaa
     2761 ttctgcttgc cgaagatgat gaaaaccttg gcctgttgct taaagagtat ttgattgcca
     2821 aaggttacga taccgacctt tttcccgatg gggaaacagc ctaccacggt ttcatgcgca
     2881 accaatataa catttgtttg cttgacgtta tgatgcctaa gaaagatggt tttacccttg
     2941 ctcgcgatat cagattggta aatgccgata ttccggttat tttccttaca gccaaaaacc
     3001 tgaaagaaga tgtgattgaa ggctttaaaa taggggccga cgattacatg acaaaaccgt
     3061 tcagtatgga agaactcatc ttcaggattg aagcaatttt gcgccgaata tcgaacgaca
     3121 accagcccag tgatcagaat gtttatcata ttggccttta ttctttcgat gtacgaaagc
     3181 aaattctttc tcacgacgga gaagaagtta agctcacaac aaaagaatcg gaattactaa
     3241 ggttattggt tgccaatgcc aacaaagtg
//
LOCUS       DAOPUW010000148         3265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_30047_length_3265_cov_2.780374, whole genome shotgun sequence.
ACCESSION   DAOPUW010000148 DAOPUW010000000
VERSION     DAOPUW010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30047_length_3265_cov_2.780374"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PKJ43_03535"
     CDS             complement(<1..282)
                     /locus_tag="PKJ43_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNZ71664.1"
                     /translation="MDSKFSPKLRDVLSYSREEAIRMGNDFIGPEHIFLGILRDGEGV
                     AIEILHGLGVDFYELKKSIEAEIKEDQILDSGASIQLLKTTEKTLKLVYL"
     gene            498..3017
                     /gene="gyrA"
                     /locus_tag="PKJ43_03540"
     CDS             498..3017
                     /gene="gyrA"
                     /locus_tag="PKJ43_03540"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNZ71665.1"
                     /translation="MSENERILKINIEEQMKSAYIDYSMSVIVSRALPDVRDGLKPVH
                     RRVLFGMSELGVLSNRPYKKSARIVGEVMGKYHPHGDSSVYMTMVRLAQNWSLRYPLV
                     DGQGNFGSVDGDSPAAMRYTEARLSKIAEETLQDLEKNTVDFQPNFDESLKEPTVLPT
                     RIPNLLVNGASGIAVGMATNMPPHNLIDTIDAINAYIDNSDIEIDDLIEIIKAPDFPT
                     GGIIYGYQGVKESYQTGRGRIIVRGKANIETLGNGREKIIISEIPYMVNKAELIMKIA
                     ELVNEKKIEGISNVNDESDRSGMRIVIDLKKDEIANVVLNKLYKYTALQSSFSVNNIV
                     LVKGRPRLLNLKQLIKYFVEHRLDVVTRRTQFELDQAEKRAHILLGLIIASDNIDEVI
                     SIIRSSNNPDEARERLMARFELSDIQARAIVEMRLRQLTGLEQEKLRAEYEELLKLIE
                     RLKEILADVSLRMNIIKEELNELKEAYPEGRRTEIVPNAEEFNPEDFYADEEMVITIS
                     HLGYIKRTSLSEFKTQGRGGVGSRGGATRDEDFLEHMFVATMHNTLLLFTEKGKCFWL
                     KVYQIPEGSRNSKGRAIQNLLNIESDDKVMAYINVLTLDDSDYINNNNIILCTKNGII
                     KKTLLEAYSRPRQNGVNAITIREGDQLLEAKLTNGSHEIMLAVKSGKAIRFNENTVRA
                     IGRTASGVRGVNLAEGDEVIGMICVENESEDVMVVSENGYGKRSKIEDYRITNRGGKG
                     VKTINITEKTGKLVAIKSVTDSDDLMIITADGITIRMAVNSVRLMGRTAQGVKLINLR
                     ENSTIASVASVSKNEENEDIEEAGQPNDYDIENEAEATAEE"
     gene            3071..>3265
                     /locus_tag="PKJ43_03545"
     CDS             3071..>3265
                     /locus_tag="PKJ43_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71666.1"
                     /translation="MRRITTLFILLLFVTSVFAQRGKVTSALSYKESGEILKAYQTIQ
                     EAIDPSNEKTAKSITWPRTWE"
BASE COUNT         1087 a          630 c          684 g          864 t
ORIGIN      
        1 aagataaacc agttttaatg tcttttctgt agttttcaac aactgtatcg atgcccccga
       61 atcaagtatc tggtcctctt taatttctgc ttcaattgat tttttcaatt catagaaatc
      121 aacaccaaga ccatgcagta tttcgatggc tacaccttca ccgtcgcgca aaataccaag
      181 aaaaatgtgc tcaggcccaa tgaaatcatt gcccatacgt attgcctctt ctcggctata
      241 tgataataca tcccttaatt ttggagaaaa ttttgagtcc atatattctt taacaatatt
      301 tgtttttaca attgagtcac taatatacac tattttcaaa atagaatctt tcagcaaaga
      361 ccaattaaat agcaattgtt gaaagattgg taaagtatgt taatattttc gacaattggc
      421 aacaagaatc tactgtttag tcacggtatt tttgtatttt taaccgcttt ttttgagact
      481 tacgaaacac tattataatg tctgaaaacg aaagaatttt aaaaataaac attgaggagc
      541 aaatgaaatc ggcctacatc gactattcga tgtcggttat cgtttctcgt gctttacccg
      601 atgttagaga tggattaaaa cctgttcacc gccgtgtttt attcggaatg agcgaactgg
      661 gagttctgtc aaaccgccca tataagaagt cggccaggat tgttggtgaa gttatgggta
      721 aatatcaccc acatggtgat tcatcagtgt atatgacaat ggtgcgttta gcccagaatt
      781 ggtcactaag gtacccgtta gtcgacgggc agggaaactt cggctcggtc gatggcgaca
      841 gcccagctgc tatgcgttac accgaagctc gcttatcgaa aattgccgaa gaaacgctcc
      901 aagacctcga aaaaaataca gttgactttc aacctaattt cgatgaatcc ctcaaagaac
      961 cgacagttct gccaacgcgt atacctaact tgctggtgaa cggagcttcg ggtattgctg
     1021 ttgggatggc taccaatatg ccacctcaca acctcatcga taccattgat gcaattaatg
     1081 cctatataga taacagcgat atcgaaatcg atgacctgat cgaaattatc aaggcccctg
     1141 attttcctac cggaggtatt atatatggat atcaaggagt taaggagtca tatcaaaccg
     1201 gtcgtggacg tataattgtt cggggcaaag ccaacatcga aacgttgggc aacggtcgtg
     1261 aaaaaatcat catctccgaa attccttaca tggtcaataa agcagaattg atcatgaaaa
     1321 ttgcggagct ggtcaatgaa aagaaaatcg aaggcatctc aaacgtaaac gacgaatcag
     1381 acagaagcgg tatgcgcatc gtaatcgatt tgaagaaaga cgagatagcc aatgttgtgc
     1441 tgaacaaatt atataaatac actgctctgc agtcatcttt cagtgtaaac aacattgttt
     1501 tggttaaagg acgtccaaga ctactgaatc tcaaacaact cataaaatat ttcgttgaac
     1561 accggctcga tgtggtaacc cgtcgtactc agttcgaact cgatcaagct gaaaaaagag
     1621 ctcacatctt gttgggacta atcattgcca gcgataatat cgacgaagta atcagtatta
     1681 tccgttcttc gaacaaccca gatgaagcaa gggaaaggtt aatggctcga ttcgaactct
     1741 ctgacataca ggcacgtgca attgttgaaa tgagacttcg ccaactcacc ggcctggaac
     1801 aggaaaagct tcgcgctgaa tacgaagaac ttcttaaact gatcgagcgc ctgaaagaaa
     1861 ttcttgctga tgtttcgctc cgtatgaata ttataaagga agaactcaac gaattaaaag
     1921 aagcttaccc agaaggacga aggactgaaa ttgtgccaaa tgccgaagaa ttcaaccctg
     1981 aagactttta tgccgatgaa gaaatggtaa taactatttc acatttaggc tacattaaac
     2041 gtacctcttt gtctgaattc aagacacagg gtaggggtgg ggttggctct cgtggaggtg
     2101 ctacccgcga tgaagacttc cttgaacata tgtttgtggc taccatgcac aataccttgc
     2161 tgttgtttac tgaaaaaggc aagtgcttct ggctcaaggt ttatcagatt cctgaaggat
     2221 cgagaaattc aaagggccgc gcaatacaaa acctgctgaa cattgaatca gacgataaag
     2281 taatggcata catcaatgta ctgacactcg acgattccga ttatatcaat aacaataata
     2341 tcatattgtg cacaaagaat ggtataatca agaaaacatt acttgaagca tattctcgtc
     2401 ctcgtcagaa tggtgtaaat gcaattacta ttagggaagg tgaccaactt cttgaggcta
     2461 aacttaccaa tggcagtcac gaaataatgt tggctgtgaa atcaggaaaa gcaatcaggt
     2521 ttaacgaaaa taccgttcga gctataggcc gtactgcttc tggtgttcgc ggagttaacc
     2581 tggctgaggg cgatgaagta attggcatga tatgtgttga aaacgagtcg gaagacgtaa
     2641 tggtagtttc agaaaacggt tacggaaaac gttcaaaaat tgaagattac cgaattacaa
     2701 acagaggagg aaaaggggtt aaaaccataa acattaccga aaaaactggt aaactggtag
     2761 ccattaagag cgtaactgac agcgatgatc tgatgattat tactgccgac ggaattacca
     2821 tccgaatggc cgttaattcg gtaaggctga tgggacgtac agcacaaggc gtgaaactca
     2881 tcaacctcag agaaaattcg acgattgcat cggttgccag cgtttcgaaa aacgaagaaa
     2941 acgaagatat tgaagaagcg ggtcaaccta atgattatga catagagaat gaagctgaag
     3001 ctacggccga agaataatag aaatcaaata attatcgtaa ttttgaaagt ttaattgaaa
     3061 caatacaact atgagaagaa ttacaacatt attcatcctt ttgttatttg taacctcagt
     3121 atttgcacaa aggggaaaag tgaccagtgc gttgagttac aaagaatcgg gagagatatt
     3181 aaaagcctat caaaccattc aggaggcaat tgacccgagc aatgaaaaaa ctgcaaagag
     3241 tattacttgg ccaagaactt gggaa
//
LOCUS       DAOPUW010000149         3263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_30071_length_3263_cov_3.144638, whole genome shotgun sequence.
ACCESSION   DAOPUW010000149 DAOPUW010000000
VERSION     DAOPUW010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30071_length_3263_cov_3.144638"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /gene="ppnK"
                     /locus_tag="PKJ43_03550"
     CDS             <1..214
                     /gene="ppnK"
                     /locus_tag="PKJ43_03550"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the phosphorylation of NAD to NADP;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HNZ71667.1"
                     /translation="VPDDVEIELIIEGRGNQFLVSVDHRSMPLDFSTSIKIKKALVNV
                     PTVKFNGHTFYSTLRNKMMWGADKRN"
     gene            395..1066
                     /locus_tag="PKJ43_03555"
     CDS             395..1066
                     /locus_tag="PKJ43_03555"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNZ71668.1"
                     /translation="MPVKKLGIVVLLIVLSCQVKAQQRSADIGLFVGGGTPLTDFTQT
                     KITESLNFDFGIFYRHNYNSRISLRFNAIYGTMNQTGTFNKQEMKFSKKVFNFDALVE
                     INYLDFIIGVPKMRFSPFVYTGIGLSTYPGKDGNTIVTPSIPLGIGAKYAFNKQLAIG
                     AEASLHKLMNDGLDNFDNPYTNNGLIKVSDIAHNNDWLSYFGLTLTYKFFWGSVPCPI
                     YETIN"
     gene            1069..1824
                     /locus_tag="PKJ43_03560"
     CDS             1069..1824
                     /locus_tag="PKJ43_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNZ71669.1"
                     /translation="MSFKEQIDSSKIPQHVAIIMDGNGRWAAKRGSDRIFGHEHGVEA
                     VRAAIKAAAEIGVKHLTLYAFSTENWNRPKFEVDALMTLLVKVIKSEASELNKNNIRI
                     NVIGDRNSLPSKVAGEVEKIESMLSQNTGLNVILALSYSSKWEIINAVKSIASDVKKG
                     KIDTQDISQELFDSYLSTANIPDPELMIRTSGERRLSNFLLWQLAYAELYFTKKLWPD
                     FDKEDFWEAICSFQNRERRFGKTSDQIKKPNIK"
     gene            1824..>3263
                     /locus_tag="PKJ43_03565"
     CDS             1824..>3263
                     /locus_tag="PKJ43_03565"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNZ71670.1"
                     /translation="MTRKFSLTALLVLIFFNLWSQIPDSTDFSIYYSSPREYEVGGIE
                     IMGIKYLDKDMLKEYAGIKVGDQITIPGDKVTQIIKKFWKQGLFSDVKVNASKIIDDK
                     IYLEIFLKERPRLSKANYHGVKKSELEDLQEKVLLLEGGQVVDAQLINAERIIKNYFK
                     EKGFLNTEVNIVQRDDPDKENSLILDINVDKKEKVKISELVFHGVEKVNVSLLERVMK
                     KTNDKKPKNFFLTKKFQEDLYEKDKISVLEKYNELGYRDAIIVRDTVIKNENDNTVKV
                     ELWIEEGNKYHIRNFKWVGNTIYPSPLLENYLGIRKGEVYNQKRLQKRLSTDNDALSN
                     LYLDKGYLFSSIIPVENYAEGDSIDFEMRISEGTQATIDRVIIQGNTKTHEQVARREL
                     YTLPGDLFSKTQLMRSYRQLAQLGHFDQERIVPDVVPDQANGTVDIKYNLVERANDQV
                     ELSGGWGMGMFVGSLGLKFTNFSAQNIFNP"
BASE COUNT         1118 a          616 c          659 g          870 t
ORIGIN      
        1 tgtccccgat gatgttgaaa tagaattaat tatcgaagga cgcggtaatc agtttcttgt
       61 atcagttgat caccgctcaa tgcctctcga tttctcaact tcaataaaaa taaaaaaggc
      121 tttggtgaat gtaccaaccg taaaattcaa tggacacacc ttttattcta ccttgcggaa
      181 caaaatgatg tggggtgccg acaaaagaaa ctagagggaa aaagcaaatc agacagcaat
      241 ttttatccat tttgggagtt aataaagaag cccgtataat tttaaaaatt atattacttt
      301 tgctgcactc tgaaaaggag ctgtaatttg ggctactccg gaacattaca aatggttttg
      361 cttccggttt ttttaatggt taaaaaaata gaatatgccc gttaagaaac ttggaatagt
      421 agtattgtta attgtattat cgtgtcaagt caaggcccag caacgatcag ccgacattgg
      481 actgtttgtt ggtggaggta caccgttgac cgattttacc caaacaaaaa ttaccgaatc
      541 gctaaatttc gatttcggta tattttatcg gcacaattac aactcccgta tcagtttgcg
      601 ttttaacgct atttacggaa caatgaacca aacaggaaca ttcaataaac aggaaatgaa
      661 attcagcaaa aaggttttca atttcgatgc actggttgag attaactatc tcgattttat
      721 tattggtgtg cctaaaatga ggtttagccc atttgtatat accggtattg ggctaagcac
      781 gtacccggga aaagatggga acaccatagt aacgccaagt attcccctgg gcatcggggc
      841 aaaatatgcc ttcaacaaac aactagcaat tggcgctgaa gcctctttgc ataaattaat
      901 gaacgatggg cttgacaact tcgataaccc atacacaaac aatgggttga ttaaggtaag
      961 cgatatcgca cataataacg attggttaag ctattttgga ttaactttga cctataaatt
     1021 tttctggggc agtgtacctt gtcctatata cgaaaccatt aattaacaat gagttttaaa
     1081 gagcaaatag acagttcaaa aataccacaa catgttgcca tcattatgga tggaaacgga
     1141 cgctgggcag ccaaacgcgg gagtgaccgg atttttggac atgaacatgg tgttgaagca
     1201 gttcgtgcag caataaaagc tgcagccgaa attggggtaa aacatcttac cctgtatgcc
     1261 ttttcaaccg aaaactggaa ccgcccaaaa tttgaggtag atgctttgat gaccctgctt
     1321 gtgaaagtta ttaaatcaga agcatctgag ctaaacaaaa acaacatccg aatcaatgtc
     1381 attggtgaca gaaatagcct accctcaaaa gtagccggag aagttgaaaa gattgaaagc
     1441 atgctttcgc aaaataccgg gctaaatgtc atccttgccc ttagctacag ttcaaaatgg
     1501 gaaatcataa atgctgttaa atcgatcgct tccgatgtta aaaaaggaaa aatagacact
     1561 caagatattt cacaagaact gttcgatagt tatctttcca ccgcaaatat tcccgatccg
     1621 gaactgatga tcagaacctc tggtgagaga aggcttagta atttcttatt gtggcagtta
     1681 gcttatgctg agttgtattt tacaaagaaa ttatggcccg attttgataa agaagatttt
     1741 tgggaagcaa tttgcagttt tcagaacaga gaacgccgtt ttggcaaaac aagtgatcaa
     1801 attaaaaaac ctaatataaa gtaatgacca gaaaattttc gttaacagcc cttttggttc
     1861 tgatattttt caacctttgg tcacaaatac ctgatagtac agacttttcg atttactatt
     1921 ccagtccgcg tgaatatgaa gttggtggaa tagaaattat ggggatcaag tatctcgata
     1981 aagatatgtt aaaagagtac gcgggcatta aagtgggcga ccagattacc attccgggcg
     2041 acaaagtaac ccaaattatc aaaaaattct ggaaacaagg gctgttctcc gatgtgaaag
     2101 taaacgcatc aaagattatc gacgataaaa tttatcttga aatattcctt aaagaaagac
     2161 caaggctttc taaagcaaac taccacgggg taaaaaaatc ggagctcgaa gaccttcagg
     2221 aaaaagtact tctgcttgaa ggaggccagg tggtcgatgc ccaattaatt aatgctgaaa
     2281 ggattattaa aaattatttc aaagaaaaag ggtttctcaa taccgaagtc aacattgttc
     2341 aacgtgacga ccctgataaa gaaaactctc tcatcctcga tatcaacgtt gataaaaagg
     2401 aaaaagttaa aatcagtgag ctcgtatttc atggcgtcga aaaagtaaat gtttcgttac
     2461 tcgaacgtgt aatgaaaaag acaaacgaca aaaaaccaaa gaatttcttt ttaacgaaga
     2521 aattccagga agacttgtat gaaaaagata aaatcagtgt actcgagaaa tataacgaac
     2581 tgggatatcg cgatgccatt atcgtccgcg acactgttat aaaaaacgag aacgacaata
     2641 cggttaaagt agaactttgg atagaagaag gcaacaaata ccacatccgg aacttcaagt
     2701 gggtcggtaa tactatttat ccttcgcctc tcctcgaaaa ttatctgggc attagaaaag
     2761 gtgaagtata taaccaaaaa agattacaaa aacggttgtc gactgataat gatgccttaa
     2821 gtaacttata tctcgacaaa ggataccttt tttcgagcat aatcccggta gaaaattatg
     2881 ccgaaggcga ttctatcgat tttgagatgc gcatttccga aggcactcaa gccacaatcg
     2941 accgtgttat cattcaagga aatacaaaaa cacacgaaca agtcgcccgc cgcgagttgt
     3001 ataccctgcc cggagatttg ttcagcaaaa cacaattgat gcgttcatac aggcagttgg
     3061 cgcaacttgg tcacttcgac caggaacgaa tcgtacccga cgtagtacca gaccaggcaa
     3121 acggaactgt agatataaaa tacaatttgg ttgaacgcgc caacgaccag gtagagctct
     3181 ccggtggttg gggaatgggc atgtttgtag gctcattagg attgaaattc accaacttct
     3241 cggcccagaa cattttcaac ccc
//
LOCUS       DAOPUW010000150         3260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_30123_length_3260_cov_2.835257, whole genome shotgun sequence.
ACCESSION   DAOPUW010000150 DAOPUW010000000
VERSION     DAOPUW010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30123_length_3260_cov_2.835257"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /locus_tag="PKJ43_03570"
     CDS             <1..1349
                     /locus_tag="PKJ43_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNZ71671.1"
                     /translation="NMAPADIRKEGSSYDLPIAIGILAASEQIKADKLDRFVVMGELS
                     LDGSLQPIRGVLPIAIKARQEGFEGFILPKANAREAAVVDKLKIYGADSISEVIEFFN
                     EEKELEQTIVDTRKEFFENVMHSDLDFSDVKGQENVKRALEVAAAGGHNIIMIGPPGS
                     GKTMLAKRIPTILPPFTLHEALETTKIHSVAGQIDSETSLMTQRPFRSPHHTISDVAL
                     VGGGTYPQPGEISLAHNGVLFLDELPEFKRAVLEVMRQPLEDRRITISRAKFSVEYPS
                     SVMLVASMNPCPCGYYNHPTRDCVCAPGMVAKYLNRISGPLLDRIDIHLEVIPVPFDK
                     LQNQPRAEGSSEIRQRVIRAREAQAFRFKDENGTYCNAQMSSKQLRTYSQPDEAGSQL
                     LKTAMEKLGLSARAYDRILKVSRTIADLEGSENVLAHHLSEAIQYRSLDRENWGEK"
     gene            complement(1363..2154)
                     /locus_tag="PKJ43_03575"
     CDS             complement(1363..2154)
                     /locus_tag="PKJ43_03575"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HNZ71672.1"
                     /translation="MKMNNLITFTVLLVLFAACQEDVVLDLNKTEKKLVVEAYVSNEF
                     PCANVSLSYSLDFYDEVIQEKLDNATVNLTDENGQVTVLDLNNAREFVPVSFIPNFGL
                     NYTLNISLGDQIIQSQATLPFPVPIVDVSFVPNPFYSSPDSLNLYVTLADQIDRDNFF
                     RLKVRRLSEEDSGEYSLFDDSFGNGGLVTLPVYFRNFSAGDTVVVELFNLSEEAFNYY
                     AWLSENDRATLNTIAPGNPTSNVPENVLGCFIAYTVDRDTVIARF"
     gene            complement(2211..>3260)
                     /locus_tag="PKJ43_03580"
     CDS             complement(2211..>3260)
                     /locus_tag="PKJ43_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ71673.1"
                     /translation="SQKQGIETAAYISNEQKVGSRFSIGYGLRVSGFTRVGKGTEYQF
                     DVKGELADSSMYGEGEFYSPYWYVEPRLNTSLILNENASIKAGYNRLSQYIHLLQNAT
                     SGTPIDLWVPSSPNVKPQLADQVSLGFFRNFNNHIYQFSAETYYKWMYNQIDYKTGAE
                     IILNEMVEGELLYGTGKAYGLELMLEKAEGKLTGWISYTLSRSLKQIDGINNNYWYPA
                     RQDRIHDLAIVGIYRLNPKWTISANWIYYTGNAVTFPAGKYYFDGKTVNLYTERNGYR
                     MPDYHRLDIGATWLLAEKKNYRSELNFSVYNAYGHKNAYNYIFTEDSDNPGKTKTVMV
                     YLFSVLPSVTWNFKF"
BASE COUNT         1007 a          754 c          635 g          864 t
ORIGIN      
        1 tcaatatggc tccggccgat attcgcaagg aaggctcatc gtacgacctg cccattgcca
       61 ttggcatact agccgcttcg gaacaaataa aagccgataa actcgaccgt tttgtagtta
      121 tgggagaact ttcactcgac ggcagcctgc agcctattag aggggtactt cccatcgcca
      181 taaaagcacg gcaagaaggg ttcgaaggct ttattcttcc aaaagccaat gcgcgagaag
      241 ccgcagtagt tgataaactc aaaatttatg gtgctgattc aatttctgaa gtgattgaat
      301 tcttcaacga agaaaaagag ctggaacaga ccattgttga cacccggaaa gaattttttg
      361 aaaatgtgat gcattccgac ctcgatttct ctgacgtgaa gggtcaggag aacgtcaagc
      421 gcgcccttga agttgctgca gccggtggcc ataacattat tatgattggc cctcccgggt
      481 caggaaaaac catgctggca aaacgcatac ctaccatatt acctccattt acccttcacg
      541 aagccctcga aacaacaaaa atccattcgg ttgccggaca aattgacagt gaaacatcgc
      601 ttatgacaca acgaccgttc agaagcccgc accacactat ttctgatgtt gcccttgtgg
      661 ggggcggaac atatccccag cccggcgaaa tatcgttagc tcacaacggg gtactctttc
      721 tcgacgagct tcctgaattt aaacgagcgg tattagaagt tatgagacag cccctcgaag
      781 accgaagaat tacaatctca cgggcaaaat tttcggttga atacccttca agtgtgatgt
      841 tagtagccag tatgaaccca tgcccatgtg gctattacaa tcacccaact cgcgattgcg
      901 tatgtgcacc cggaatggta gccaaatatc taaaccgcat atcgggtccg ctcctcgatc
      961 gtatcgatat ccatctggaa gtaattcctg tgcccttcga caaactacaa aaccaacccc
     1021 gtgccgaagg cagtagcgaa atacgccaac gtgtaatacg agcacgcgag gcacaagctt
     1081 ttcggtttaa ggatgaaaat ggcacctatt gcaatgcaca aatgagctca aaacaactcc
     1141 gaacatactc gcaacccgat gaagccggtt cgcaattatt gaaaacagcg atggaaaaat
     1201 tggggttatc ggcccgtgcg tacgaccgta ttctaaaggt ttcgcgcaca attgccgatt
     1261 tggaaggaag cgagaacgtg ctggcacatc acctttcgga agctattcag taccggagcc
     1321 tcgaccgtga aaattgggga gagaaatagc aatacaaaac acctaaaatc ttgctattac
     1381 agtatcgcga tcgacagtgt atgctataaa acaccctaaa acattttcgg ggacattgct
     1441 tgttggattt ccgggtgcaa tagtattcaa tgttgcccgg tcgttttccg aaagccatgc
     1501 ataataatta aaagcttcct cactaaggtt gaacaattca acaacaacag tatctcctgc
     1561 agaaaagttt ctgaaataca cgggcaatgt taccaatccc ccattcccaa aagaatcatc
     1621 aaacagtgaa tattcgcccg aatcttcttc gctcaatcta cgaactttca gcctaaaaaa
     1681 gttatcccga tcaatttgat cggcaagcgt tacatacaaa tttaaactgt cgggagaact
     1741 ataaaacgga ttgggcacga aagagacatc gacaattgga accggaaacg gcaacgtggc
     1801 ttgcgattgg ataatctgat caccaagact tatgttcaac gtataattaa gaccaaaatt
     1861 ggggataaaa ctaacgggca caaactctct cgcattgtta agatctaaaa cagtaacttg
     1921 accgttttcg tcagtgaggt ttactgtggc attatccaat ttctcttgaa ttacttcatc
     1981 gtagaaatcc agactatatg ataaagaaac atttgcacaa ggaaattcat tgctcacata
     2041 cgcctcaact acaagttttt tttcagtctt gttcaaatcg agaaccacat cttcctgaca
     2101 tgcagcaaac aaaaccagaa gtactgtaaa tgtaataaga ttgttcattt tcattgccaa
     2161 aaacttacta taatcattta tattctgaat tatttcggga ttataccctg ctaaaattta
     2221 aaattccaag taaccgaagg gagtaccgaa aacaaataaa ccattacggt ttttgttttt
     2281 cctggattat cagaatcttc ggtaaaaata taattataag cattcttgtg tccataggca
     2341 ttgtagactg agaaattcaa ctcagaccga taattttttt tctccgccag aagccatgta
     2401 gcgccaatat cgaggcgatg ataatcaggc atcctgtatc cgttacgctc ggtgtataga
     2461 tttactgttt tcccatcaaa ataatacttt ccggcaggga aagtaacagc attcccggta
     2521 taataaatcc agttggccga gatagtccac ttaggattta accgatagat tccaacgata
     2581 gccaaatcgt gaatacggtc ttgcctggcc gggtaccaat aattgttgtt aatcccatcg
     2641 atttgcttta aggaacggga taacgtatat gaaatccaac cggttaattt tccctcagct
     2701 ttttccaaca tcaattcaag cccataagct ttaccggtac catacaggag ttctccctca
     2761 accatttcgt ttaaaattat ttcggcacca gtcttataat caatctgatt atacatccac
     2821 ttataatatg tttcggcaga aaactgatag atatgattgt taaaattccg aaagaaaccc
     2881 aagctgacct gatcggctaa ctgtggtttt acatttggag aactgggcac ccacaaatcg
     2941 ataggagtac cggaagttgc attctgcaat aaatgaatat attgcgataa ccgattataa
     3001 cccgccttta tgctggcatt ttcatttaga attaatgagg tattcagccg aggttcgacg
     3061 taccagtatg gactataaaa ttcgccctcc ccgtacattg aagaatcggc aagctctcct
     3121 ttaacatcaa actgatattc cgtacccttt ccaactcgtg taaatccaga cacacgcagt
     3181 ccgtacccaa tgctaaacct tgatccaact ttctgttcat ttgagatata tgcagcagtc
     3241 tcaatgcctt gcttttgaga
//
LOCUS       DAOPUW010000151         3253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_30206_length_3253_cov_3.809256, whole genome shotgun sequence.
ACCESSION   DAOPUW010000151 DAOPUW010000000
VERSION     DAOPUW010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30206_length_3253_cov_3.809256"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PKJ43_03585"
     CDS             <1..817
                     /locus_tag="PKJ43_03585"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNZ71674.1"
                     /translation="ASAIISLIAYVGIINAYNLIDGIDGLATMLSMLAAATFGTLFII
                     KGDMAYAILAFATIGSLASFFFYNVFGKKNKIFMGDTGSLLLGLLIAIFVIHYNQLTL
                     NGPLAEQLLAPVLSMAVVSIPLFDMVRVFGLRIMKGKSPFFPDMNHIHHKLLKLKMSH
                     LRSTLTLVIINLFFIGLAYLIAPIDNTLQLVIMFTVAILLSLVPGEIFRLRKAAEIEE
                     IIEKENIIPENTKAIFFNISKQKSATTDIKAGISSEKKETAKAEKLELEHFNV"
     gene            1116..2270
                     /gene="gmd"
                     /locus_tag="PKJ43_03590"
     CDS             1116..2270
                     /gene="gmd"
                     /locus_tag="PKJ43_03590"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNZ71675.1"
                     /translation="MKTALITGITGQDGAYLAEYLLKKGYIVHGIKRRASLFNTDRID
                     HIYQDPHVENRNLYLHYGDLTDSMNLTRIIQETQPDEIYNLAAMSHVQVSFETPEYVA
                     NADGIGTLRILEAVRLLGLTKKTRIYQASTSELYGLVQQVPQTEKTPFYPRSPYAVAK
                     MYAYWITVNYREAYGMHANNGILFNHESPIRGETFVTRKVTRALSKIALGLQETVFMG
                     NMNSKRDWGHAKDYIRAMYLILQQDKPDDYVIATGITTTIRDFISKAANEIGLEIEFR
                     GQNADEIGVLVNVDQQKFAEKVGDKYLDQIVVRAAQKDVVVGVDKRYYRPTEVDLLIG
                     DPTKSKTVLGWEPKYDLQGLISDMMQSDIKLMQKEDYLREGGYKIMNYFE"
     gene            2270..>3253
                     /locus_tag="PKJ43_03595"
     CDS             2270..>3253
                     /locus_tag="PKJ43_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNZ71676.1"
                     /translation="MKKVFVSGCYDLLHSGHVAFFKEAAQYGDLYVGIGSDATIAELK
                     GRQTINSEQERLYMVKSVRYVKEAFVNAGSGIMDFENELRTLKPEYFVVNEDGFSPAK
                     KQLCDELGIELKVLERIPDAGLPARSTTAIRSAGNCTLPYRIDLAGTWIDQPYVSKHY
                     PGWAITLSLEPIIEYNERCGMSTSTRNAAKKIWPYHLPLEKPERLAEILFKFENEPGS
                     SIISGAQDAIGICMPGLVRHYYDSAYWPKKFESVYDEDILSWLEQHIFMVLLWPREKD
                     LDLLKETYITTENVKRLADAAEKAWNAILEKDIHRFAEAFLDSFRAQTQMFP"
BASE COUNT         1028 a          668 c          635 g          922 t
ORIGIN      
        1 tgcaagtgcg ataatatcac tcatcgcata cgttggaata ataaatgctt ataacctaat
       61 cgacggaatc gatgggttgg ccacaatgct cagtatgctt gctgctgcaa cctttggtac
      121 ccttttcatc ataaaaggtg acatggctta tgccattttg gcctttgcca ccattgggag
      181 tttagcaagt ttcttctttt ataacgtttt cggtaaaaag aataagattt tcatgggcga
      241 tactggctcg ttactcttag gtcttttaat agctattttt gttatccatt ataaccaact
      301 aaccctaaac ggaccactag cagaacagct tttggcacca gtactttcaa tggcagttgt
      361 ttcaattccg ctattcgata tggttcgagt tttcggatta agaattatga aaggtaaatc
      421 accttttttt cctgatatga accacattca tcataagttg ctaaaactta aaatgtctca
      481 cttaagatca acattaactc tagttattat caatttgttt ttcattggat tagcatatct
      541 tattgctcct atcgacaata ctttacaatt ggtaatcatg tttactgtag caattctttt
      601 gtcgttggtt ccgggcgaaa tattccgttt aaggaaggct gctgaaattg aagaaatcat
      661 tgaaaaagaa aatattattc cagaaaacac gaaagcaatt tttttcaaca taagtaaaca
      721 gaaaagtgca acaacagata ttaaggcagg tatttcaagc gaaaaaaagg agactgcaaa
      781 agctgaaaag ctagaattgg agcattttaa tgtatagaat ctactataag ttgtttttct
      841 caagcgcttt tttcaagagt tactatgtta tactatgatt aattattatc caactactgt
      901 ttaaataata attaatcata tgtgaaattc aaatcgataa attgatttca atgaatcact
      961 actaaaccca gaaatgcatt aataaattac tcctaaagac attgcatttc caagatataa
     1021 cattttcttt ttcttacttt tgaacaattt tcaaagagca ttacctttga atgcattttt
     1081 ttagaaaata agctaacaat caacaaaata cacaaatgaa aaccgcacta attaccggta
     1141 ttaccggaca agatggcgct tatcttgctg agtatctatt aaaaaaagga tacatcgttc
     1201 atggaataaa acgaagggct tcgctgttta ataccgacag gatcgatcac atctatcagg
     1261 atccccatgt tgaaaaccgt aatctttacc tccattatgg cgaccttacc gatagcatga
     1321 accttacacg tatcattcag gaaactcaac ccgacgaaat ttataacctt gctgcaatga
     1381 gccatgttca ggtaagtttc gaaacccccg aatatgttgc aaacgccgat ggaatcggca
     1441 cattaaggat tcttgaagcg gtacggcttc tgggattaac aaagaaaaca aggatttatc
     1501 aggcctctac ttccgaatta tacgggcttg ttcaacaagt gccacaaacc gagaaaaccc
     1561 cgttctatcc tcgttcgcct tatgcagttg caaaaatgta tgcctactgg atcactgtca
     1621 actatcgcga agcgtatgga atgcatgcca acaatggaat cctcttcaac cacgaaagcc
     1681 ctatccgtgg cgaaacattc gttacacgta aagtcaccag ggcgctttca aaaattgcgc
     1741 ttggattaca ggaaactgtc ttcatgggca acatgaacag taagagggat tgggggcacg
     1801 ctaaagacta tattcgtgca atgtacctca tcctgcagca ggataaacca gacgattatg
     1861 tgattgctac cgggattaca acaactatac gcgactttat tagcaaagca gccaacgaaa
     1921 ttggtttgga aatcgaattc cgcggtcaga atgccgatga aattggagta ttggtcaatg
     1981 tcgatcagca aaaattcgct gaaaaagtgg gagacaaata cctcgatcag atcgttgtta
     2041 gggctgcaca aaaagatgta gtggtgggcg ttgataaaag atattatcgg ccaacggaag
     2101 tagatcttct cattggcgac ccaaccaaat caaaaacggt actcggctgg gaaccaaaat
     2161 atgatcttca agggttaatt tccgacatga tgcaatccga catcaagctc atgcaaaagg
     2221 aagactacct tcgcgaagga ggatacaaaa tcatgaacta tttcgagtaa tgaagaaagt
     2281 ttttgtttct ggttgttacg acctgctcca tagtggacat gtagcttttt ttaaagaagc
     2341 ggctcagtat ggcgatcttt atgtaggcat tggctccgat gctactattg ccgaactcaa
     2401 aggccggcaa accatcaata gcgagcagga acgtctgtat atggtaaaat cggtccgtta
     2461 cgtgaaagaa gcttttgtca atgccggcag tggaatcatg gactttgaaa acgagctccg
     2521 tacattaaaa cccgagtatt tcgtagtaaa tgaagatggt tttagtccag caaaaaaaca
     2581 actctgcgac gaactgggca ttgaattaaa ggtgctggaa cgtattcccg atgctggact
     2641 tcctgctcgg agtacaacag caatacgaag tgcaggaaac tgcacccttc cctatcgcat
     2701 cgaccttgct ggaacctgga ttgatcaacc ttatgtgtcg aaacattatc cagggtgggc
     2761 catcactctt tcactcgaac caattatcga atacaacgaa cgttgcggga tgagtacatc
     2821 tacccgtaat gcagcaaaaa agatttggcc ctatcacctt ccgctcgaaa aacctgaacg
     2881 gttagccgaa atactcttca aatttgaaaa tgaacccggc tcttcaatca tttccggtgc
     2941 gcaggacgca attggcatct gtatgccagg cctagttcgt cattattacg acagtgccta
     3001 ctggccaaaa aaattcgaat cggtttacga cgaagatatt ctcagttggc ttgagcagca
     3061 catcttcatg gttcttcttt ggccccgcga aaaagatctc gatttattga aggaaacgta
     3121 tattactact gagaatgtaa agcgtcttgc cgatgctgct gaaaaagctt ggaatgcaat
     3181 tcttgaaaaa gacatccatc gatttgctga agcatttctc gattcattcc gcgcgcaaac
     3241 tcaaatgttt cct
//
LOCUS       DAOPUW010000152         3249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_30254_length_3249_cov_3.173450, whole genome shotgun sequence.
ACCESSION   DAOPUW010000152 DAOPUW010000000
VERSION     DAOPUW010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30254_length_3249_cov_3.173450"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /gene="lepA"
                     /locus_tag="PKJ43_03600"
     CDS             complement(<1..1014)
                     /gene="lepA"
                     /locus_tag="PKJ43_03600"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNZ71677.1"
                     /translation="MKNIRNFCIIAHIDHGKSTLADRLLQYTGTISDRDMTDQVLDNM
                     DLEKERGITIKSHAIQMKYEQDGEKYILNLIDTPGHVDFSYEVSRSIAACEGALLIID
                     STQGIQAQTISNLYLAIEHDLEIIPILNKMDLPNAMPEIVEDQIIELIGCKRSDIIRA
                     SGKTGEGVEEIMHRIVSDIPHPVGNVEAPLQTLIFDSVFNPFRGVIAYFKIENGILRK
                     GDRVKFFATDKEYVADEIGVLRMGMDPREELSTGNVGYVICNIKTAKEVRVGDTITHV
                     KNGCTQRIDGFQEVKPMVFAGVYPIESDDYEDLRAAIEKLQLNDASLTFEPESSLALG
                     FGFR"
     gene            1498..2208
                     /locus_tag="PKJ43_03605"
     CDS             1498..2208
                     /locus_tag="PKJ43_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNZ71678.1"
                     /translation="MGRAFEYRRAAKEKRWGMMSRVFPKLGKAITVAAKAGVPDPEMN
                     AALRTAIQNAKAQNMPKANIDAAIKRAAGKDTEAFMEELYEGKGPYGVMILVECLTDN
                     HARTVANMKWIFNKWGAGLAPSGSMLNMFSRKAVIQFEMPAGKDREELEMELIDAGLE
                     ELSENEGTCFAYADYTDFGSLTKAFEDLKIETTKATLQYIPVAPVELSEEQMEEIEKL
                     IDKIEEDDDVQQVFTNIA"
     gene            2963..>3249
                     /locus_tag="PKJ43_03610"
     CDS             2963..>3249
                     /locus_tag="PKJ43_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71679.1"
                     /translation="MKKISKILILSFLCFFTLSSFAQLQASLIGPSTVTSGGTVVQGP
                     LNAIDVPENSVLNYLPSGWTGGYTSDSKSSPSYKCFQYFTGATGSFNTVTI"
BASE COUNT         1020 a          642 c          634 g          953 t
ORIGIN      
        1 acgaaagcca aacccaaggg cgagcgacga ttccggctcg aaggtaagcg atgcatcgtt
       61 gagttgcagt ttctcaatag ctgcccgcaa gtcttcgtaa tcatcacttt caattggata
      121 aactcctgca aaaaccatgg gcttaacttc ttgaaaacca tcaattcgct gagtacaacc
      181 attcttgaca tgcgtgatgg tgtcaccaac cctcacttct ttggctgttt ttatattgca
      241 aattacatag cccacatttc cagtggagag ctcttcgcgc ggatccattc ccatgcgcaa
      301 aacaccaatt tcgtcggcaa catactcttt gtcggtagca aaaaacttca ccctgtcgcc
      361 cttgcgcaat attccatttt caattttgaa ataggcaata accccacgga atggattaaa
      421 aaccgagtcg aagataagcg tctgtaacgg agcctcaaca tttcctacag ggtgcggaat
      481 atcgctgaca atccggtgca ttatttcttc aacaccttcg ccggttttcc cgctggcgcg
      541 aattatgtcg ctgcgtttgc agccaataag ttcaataatt tggtcttcaa caatttcggg
      601 catagcattg ggcaagtcca tcttattaag gattggaata atctcgagat cgtgttctat
      661 agccagatag aggttggata tagtctgagc ctgaatacct tgcgtagaat caattataag
      721 taacgcccct tcacaagctg caattgagcg cgacacttca tacgaaaaat cgacatgccc
      781 cggggtatca atcaaattga gaatatattt ttctccatct tgctcatact tcatttgaat
      841 agcatgactc ttaatggtaa ttcccctttc cttttcaaga tccatgttat cgagcacctg
      901 gtcggtcata tcgcgatcgc tgattgttcc ggtatattgt aacaagcgat cggctagtgt
      961 gcttttcccg tgatcgatat gagcaatgat gcaaaaattt cgtatgttct tcactgtgta
     1021 tttttttatt tctttttcaa ccctcgacaa tgccggtacc aaagagtatt ttcagcagaa
     1081 aacctttggc taattaactg atataccgaa ctgtctattt ttcaaacttc gcaaagataa
     1141 cgcatttttt gaatcgggaa acagggtaaa accaagcaac aggctaaacc tactttcaaa
     1201 ataaaaaatg attaacaaaa ccatattccc ggggctcaat ttgcttttca cccgggaaaa
     1261 aatgtaggaa aagtaacagt atctatccca ttgagataaa aaagtgcatt tttcatcatt
     1321 aaagaagtga ttttattgtt ctcttattga gtgaagtcga atagattttt ttattttcgt
     1381 agcctgaaaa aattagaata cttcacaaag aaataagtaa caaatcactg gttttcaatt
     1441 attttataca ggtaccgaca tgtattaaaa ttgaaatctg taaattgata aaatattatg
     1501 ggaagagcat ttgaatatcg ccgtgctgcg aaagaaaaac gttggggcat gatgtcgcgg
     1561 gttttcccca aacttgggaa agcaattact gtagctgcca aagccggggt tcccgaccct
     1621 gaaatgaacg ctgctctaag aacagccatc cagaatgcga aggctcaaaa catgcccaag
     1681 gcaaatatcg atgctgccat taaacgagct gccggtaaag atactgaagc ctttatggaa
     1741 gaattgtacg aaggcaaagg tccttacggg gtaatgatcc ttgttgaatg cctgaccgac
     1801 aaccatgccc gtactgttgc caatatgaaa tggattttca acaaatgggg tgccgggtta
     1861 gccccttctg gctcgatgtt gaacatgttc tctcgcaaag cggtgatcca gtttgaaatg
     1921 ccagcaggca aagatcgcga agaacttgaa atggaactca tcgatgccgg gcttgaagaa
     1981 ctctcggaaa acgaaggtac ctgtttcgcc tacgctgatt acaccgattt tggttcgctc
     2041 accaaagcat tcgaagatct gaagattgaa accactaagg ctacgctcca gtacattccg
     2101 gttgctcctg ttgaattgag cgaagaacag atggaagaaa tcgaaaaact gatcgacaaa
     2161 atcgaagaag acgatgatgt acaacaagtg tttacgaata ttgcttagaa gacgttagat
     2221 aaaagatata ggaagagaag gcaacgcgaa agtgttgcct tttttatgct tttctccctt
     2281 tattcgtaaa caaaaaaagg atcaaatgaa ctttctggtt gagaaggaat attttttatg
     2341 gatcaaatga acttttgcgt gggaagggga aaaattacaa tgaaaataag cgaacctacg
     2401 ataagttaag ttccgaagga acgggtaatt ttgtaacgcc ggattttaat ccggggtaaa
     2461 acagcaacaa aagacatagt gagttccgta ggaacgaaac attaaaaata aaaaacaatg
     2521 gcaaatacat tttcccaagt ttatgtccaa tgtgtttttg cagtgaaatg tatgatgagc
     2581 ggtatatttt tgctgattta gtctaaaatg taccgagcct acagctctca ataatgtaga
     2641 ctagttgcct cattgaatgg actaaagtcc atccttacaa aatgattcga gcctacggct
     2701 ctaaattttt agatagtttt tctgactcat caaataaaag gtgcgttttc attaagtcag
     2761 aatctgcaat acccaaagag atgtcagaga aattcaattt ttcttgtccc aattagctaa
     2821 gatttatgcg tgaaaatgat ttactacacc agaaagttct cttgatccca atgttcatga
     2881 taatgtttct ataagtaatt tattagtaaa agttttttta actttgttgc gaaaatagct
     2941 aaaagaatta aatctattca acatgaaaaa aatcagtaaa atattaattt tgagtttttt
     3001 atgctttttt acattaagct catttgctca attgcaagcc agccttattg gcccttcaac
     3061 ggtaacttct ggaggtacag tagttcaagg accacttaat gccatagatg taccagaaaa
     3121 ttctgtatta aattatttgc cctcaggatg gacaggtgga tatacttccg attctaaaag
     3181 tagtccttcc tataaatgtt ttcaatattt tactggagca acaggttctt tcaatactgt
     3241 cactatttg
//
LOCUS       DAOPUW010000153         3249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_30256_length_3249_cov_2.989981, whole genome shotgun sequence.
ACCESSION   DAOPUW010000153 DAOPUW010000000
VERSION     DAOPUW010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30256_length_3249_cov_2.989981"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /gene="kdsB"
                     /locus_tag="PKJ43_03615"
     CDS             complement(<1..477)
                     /gene="kdsB"
                     /locus_tag="PKJ43_03615"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNZ71680.1"
                     /translation="MKKPLIVIPARYASTRFPGKPLALIHGKPMIQHVYQRCREVCNQ
                     VVVATDDDRIASAVKGFGGEYIMTSPDHQSGTDRCAEAVASYASKADFNLVINVQGDE
                     PFVEATQLEKIIACFDDPSCEIATLVAPISSSDVLFNSNKVKVVLSTKNYALYFSRQ"
     gene            925..2181
                     /locus_tag="PKJ43_03620"
     CDS             925..2181
                     /locus_tag="PKJ43_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNZ71681.1"
                     /translation="MIDIQDIKQRFGIIGNAPALNRAIEVAVQVAPTDLSVLITGESG
                     VGKESFPQIIHQFSSRKHGRYIAVNCGAIPEGTIDSELFGHVKGSLTGALSDRKGYFE
                     VADGGTIFLDEVGELPLSTQVRLLRVLEAGEYIRVGSSDVLKTNVRVVAATNLDIPKA
                     INNGKFREDLYYRLNTVPVQVPPLRERRDDIYLLFRKFTTDFATKYRMPPIKLDEEAK
                     HILTSFPWPGNVRQLKNITEQISIIEKEREISGITLRGYLPHFHEHQLPAIYQKESEN
                     TFSNEREILYKVLFDMKKDMTDLKKLVLDLMQHGEDSPKVKSENARIITKLYQEDELD
                     INVEQIPEVKLQKVSQPTPVIAKPEHLIQDTEEYIEETLSLEEKEKELIQKALSKYSG
                     KRKYAAQELGISERTLYRKIKEYNIN"
     gene            2188..2700
                     /locus_tag="PKJ43_03625"
     CDS             2188..2700
                     /locus_tag="PKJ43_03625"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HNZ71682.1"
                     /translation="MKRMMKFLLAAGLVVAFIGCKINYSFTGASISPDVKTYSILDFP
                     NRAKLVNPNLSNYFVEKLREKFTRQTSLNYLDEGGDLEFEGTISQYDVQPVSIKAGTD
                     EASMNRLTIKISVKFTNRKNSEQDFESEFSAYADFDASQLLSDVEDSLIEQIIKEIID
                     DIYNKSVANW"
     gene            2705..>3249
                     /locus_tag="PKJ43_03630"
     CDS             2705..>3249
                     /locus_tag="PKJ43_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71683.1"
                     /translation="MNSQQFIQILKKPETLDQSTLPALKEMIDNYPFFPLARILYLRN
                     LKNINSYKFEEELSKHAVFIPDRAALYRFLFFPARIDDGFELLPFDNESINKLMEEAE
                     FSGEEQSLSDDFYIQTTEAIRLIEPEDNLLNEKKSLEPDLIDKFIAAGTTKDGFRQAK
                     KEDERDLADQSSIVGEELITET"
BASE COUNT         1085 a          709 c          633 g          822 t
ORIGIN      
        1 ctgccgactg aaataaagtg catagttttt cgtcgacaat acaactttaa ccttattgga
       61 attaaaaaga acgtcggaag aagaaatggg cgcaaccaaa gtggcaattt cgcacgaagg
      121 atcatcgaaa caggcaataa ttttctcaag ctgagtcgct tcaacaaaag gctcatcccc
      181 ctgaacattg atcaccaaat tgaaatcagc cttggaagca tagcttgcta cagcctcggc
      241 acaacgatca gtcccacttt ggtgatcagg ggaagtcatg atatattcac ctccgaaacc
      301 tttcacagca ctggcaattc tatcatcatc ggtggcaaca accacttggt tgcatacctc
      361 acggcaccgt tgatatacgt gctgaatcat cggttttcca tgaatcaatg ccaacggctt
      421 tcccgggaaa cgggttgaag cataacgggc cggtattacg ataagtggtt ttttcacaag
      481 tatatattct tttttattct taactaatta atttacaaat gatttatatt tcgcgtttgt
      541 gtgaatttga attaaactcg taccaaacaa catgctctca accgaaatta ttttcggttg
      601 attctcttcc gagacaaaca aaggtaatcg ataccacgta atttgcattc ggtaaattta
      661 aattaaaccg ttaaaataat gaattgaaac agaattctta aatacaataa atacacactc
      721 taacattttc acccaatggt tttatttata acatgatggc tgattaaaaa cgtcattaca
      781 actttatatc gacattaaaa cgtcaactct gctaaaaaaa ctaaaaataa cagaggcaaa
      841 agttttttac cccgtagtta agcttcaaaa attgtaactt tgcctactta gtaaaatcga
      901 gtgtgaatat ttgttgcaga agacatgatt gatatacaag acataaagca acgttttgga
      961 attattggca atgcccccgc cctcaaccgg gccattgaag tggccgtgca ggttgcccca
     1021 actgatctct cggtacttat caccggagaa agcggtgtag gaaaagagtc gttcccgcaa
     1081 atcatccatc agttttcatc gcgcaagcat ggtcgctaca ttgccgtcaa ctgtggcgca
     1141 atacccgaag gaaccatcga ttctgaactc tttgggcacg tgaaaggctc gcttaccggg
     1201 gcactttccg atcgcaaagg gtatttcgaa gtagccgacg gtggtacaat ttttctcgat
     1261 gaagtaggag aactgcctct ttcgacccaa gtgcgcttac tccgtgtgct cgaagccggg
     1321 gaatacatta gggtgggttc ttctgacgtg ctgaaaacaa atgttagggt tgttgcagct
     1381 acaaacctcg acatccctaa ggcaattaac aatggtaaat ttcgcgaaga tttgtactac
     1441 cgccttaata ccgtgccagt gcaagttccg ccacttcgtg aacggcggga cgatatatac
     1501 ctgctgttcc gaaagtttac aactgatttt gcgacaaaat atcgcatgcc tccaataaaa
     1561 ctcgatgaag aagccaaaca tattctcacc tcgttcccct ggcctggaaa cgtaaggcaa
     1621 ttgaaaaata taaccgaaca aatatctatt atcgagaaag agcgcgaaat atcgggcatc
     1681 acattaagag gatacttacc tcattttcac gagcatcaac tgccagcaat ttatcagaaa
     1741 gaaagtgaaa ataccttctc aaatgaacgc gagatcctgt acaaggtcct tttcgatatg
     1801 aaaaaagaca tgaccgacct gaaaaaactg gtactcgacc tgatgcaaca tggagaagac
     1861 agcccaaaag ttaaaagtga aaatgcccga attatcacta aattatatca ggaagacgaa
     1921 ctggatatca atgtagaaca aattcccgag gtcaaactcc agaaagttag ccagccaaca
     1981 ccggtaatag ccaaacccga gcacttgatc caagacaccg aagaatacat tgaagaaacc
     2041 ctatcgctcg aggaaaaaga aaaagaattg atacaaaagg ccttgagtaa atacagcggt
     2101 aagaggaaat acgcagctca ggaacttggc atttcagaac gtaccttgta tcggaaaatt
     2161 aaagaataca atataaatta gtgcccgatg aagcgaatga tgaaattttt actggccgcc
     2221 gggcttgtgg ttgcatttat tggatgtaaa atcaactatt ctttcactgg tgcatccatt
     2281 tcacccgatg taaaaactta ttcgatcctc gattttccta acagggcaaa acttgtgaac
     2341 ccaaatctca gcaattattt tgtcgaaaag ttacgggaaa aattcacccg acaaaccagt
     2401 ttaaattatc tcgacgaagg aggcgacctt gagtttgaag gaacaataag ccaatacgat
     2461 gttcaaccgg tttcgatcaa agccggcacc gacgaagctt cgatgaaccg cttaacgatt
     2521 aaaatatcgg taaagttcac caatagaaaa aactcagagc aagacttcga gagcgagttt
     2581 tcggcctatg ccgattttga tgcaagccag ctgctgagcg atgttgaaga cagtttaatt
     2641 gaacaaatta ttaaagaaat aatcgacgat atttataaca aatcggtagc aaattggtag
     2701 ccacgtgaac agtcaacaat tcatacaaat ccttaaaaag ccggaaactc tcgaccagtc
     2761 gacacttccc gcactgaaag aaatgataga taattatcca ttctttccgt tagcccgaat
     2821 cttgtacctc agaaacctca aaaacattaa tagttacaaa ttcgaggaag aactatcgaa
     2881 acatgccgtt tttattcccg acagagctgc gctgtatcgc tttctcttct ttcctgccag
     2941 aattgacgat ggcttcgaac tcttgccctt cgacaacgag tcaattaata aactcatgga
     3001 agaagcagaa ttttcaggag aagaacagag cctttccgac gatttctaca ttcaaacaac
     3061 agaagctatc cggcttatcg aaccagaaga caacctcttg aacgaaaaga aatcgttgga
     3121 acctgatctt atcgacaaat ttattgccgc tggaaccact aaagatgggt tccggcaagc
     3181 aaaaaaagaa gatgaacgcg accttgctga ccaaagcagc attgttggag aagagctgat
     3241 tacagaaac
//
LOCUS       DAOPUW010000154         3243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_30329_length_3243_cov_3.324655, whole genome shotgun sequence.
ACCESSION   DAOPUW010000154 DAOPUW010000000
VERSION     DAOPUW010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30329_length_3243_cov_3.324655"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="PKJ43_03635"
     CDS             <1..1153
                     /locus_tag="PKJ43_03635"
                     /inference="COORDINATES: protein motif:HMM:NF019260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1593 domain-containing protein"
                     /protein_id="HNZ71684.1"
                     /translation="DPAFPSAETLLSKVKSGLPVYGMQGVGEGKSSEGSEWIIKILEE
                     PDERPLWISVWGGSNTLAQALYAINQTKPKAEVEKLISKLRVYTISDQDDSGIWIRNN
                     FPTLFYIVTPGDEYGDATWTAINGCFEGANNDVVSNSWLAENIQQGHGPMGTNYPDVA
                     YGMEGDTPSWLLLINNGLNYPEHPEWGSWGGRYEKYKPDFSKQKKGTSGVPFEPETRE
                     IWTNASDEYTPYVFNNFGRTVKISSNSYSGDKVTLWRWREDFQHDFAARMDWCIKPYS
                     EANHPPVVKLAIDEEITVKSGQGFSLIASGSYDPDGDNISYLWFSYPEAGSYKKKISI
                     NSAENMYKAWVIAPVVDKKETAHFILKVSDKGTPSLSRYKRVIVTILPE"
     gene            complement(1167..1796)
                     /gene="pdxH"
                     /locus_tag="PKJ43_03640"
     CDS             complement(1167..1796)
                     /gene="pdxH"
                     /locus_tag="PKJ43_03640"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HNZ71685.1"
                     /translation="MDLSDIRRNYGKNRLDADGVDTDPLRQLALWLAEAENADCEEHS
                     AMILSTVGTDGQPSSRVVLLKFLTDESLVFFTNYSSRKGQEISLNPKVSACFFWPSLE
                     RQVRVEGQVSKTDVKISDQYFSSRPYESRVSAVISPQSDEIPDRKFLDDQRKHLLESG
                     EPLIRPENWGGYMLNPSYFEFWQGGPHRLHDRIVYRLCSGVWETARLAP"
     gene            1863..2765
                     /locus_tag="PKJ43_03645"
     CDS             1863..2765
                     /locus_tag="PKJ43_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71686.1"
                     /translation="MKKNKAMQSNSKFGIKDIVVILLVIVLAVMVVLYFTKRNEHNKI
                     VSELNAEKDSIKVELSQMLVNYDSLKTNNEQLNENLLMTQSEIKNLLTEIEQVKRASV
                     IEVSNYKDKVNTLRGIMKDLYNQIDSLNERNKILYAENIEVKQRYTAVQSKNEQLTKE
                     KDELAQTVKMAQILEALSLRISGLNPRDKETDRISKTQKIMVSFTLSKNLTAVRGAKN
                     IYVRIMRPDQLLLVESQTALFEFEDLKIPYSAKREVNYEGNELPVNIFWDNSGHEPLI
                     PGTYTIDVFADGYNIGTTKYLFKN"
     gene            2779..>3243
                     /locus_tag="PKJ43_03650"
     CDS             2779..>3243
                     /locus_tag="PKJ43_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71687.1"
                     /translation="MENPVNNSGNETDICFHGINLTGALVALAALHSGCKVSIELNQK
                     PDLQFLPELVALYPISAFELPNSLSRIKLLKKIAGFYPTLVYPQRLLTLVEDTRLKAR
                     KLSFFDLLLGREREDASMPIRLSKYDQYQSIEPFFENGAMISEFRFDRNMLLI"
BASE COUNT         1051 a          680 c          692 g          820 t
ORIGIN      
        1 cgatccggct tttccaagtg cagaaacgtt gctatcgaaa gtaaaatcgg gacttccggt
       61 ttacgggatg caaggtgttg gcgagggaaa aagctctgaa ggatcggaat ggattataaa
      121 aatattggaa gaacctgacg aaagaccgct ttggatatca gtttggggag gatcgaacac
      181 tctggcgcaa gctctttatg cgatcaacca aaccaaaccg aaagcagaag ttgaaaaact
      241 gatttcgaaa ttacgagttt atacaatttc agatcaggat gatagcggaa tttggatccg
      301 aaataacttt cctacccttt tttacattgt aactcccggc gatgagtacg gagacgcaac
      361 atggactgct atcaatggat gttttgaagg tgcaaacaac gatgtggtga gcaatagctg
      421 gttagctgaa aacattcaac aagggcatgg cccaatggga acaaactacc ccgatgtagc
      481 gtatggaatg gaaggcgaca ccccctcgtg gttattgctg ataaacaatg ggttgaacta
      541 tcccgaacat cccgaatggg gtagttgggg tggacgatat gaaaaatata aacctgattt
      601 ttcgaaacag aaaaagggga cttcaggggt tccttttgaa ccagaaaccc gagaaatttg
      661 gacgaatgcc agcgatgaat acacccctta cgtttttaac aacttcggac gaacagttaa
      721 gattagttca aattcctatt caggggataa agttacgctc tggcgctgga gagaagattt
      781 tcagcacgac tttgcagccc gaatggactg gtgtattaaa ccttacagcg aagccaacca
      841 ccctccggtt gtgaaactgg ctattgatga agaaattaca gtgaaatcag gacaggggtt
      901 tagccttatt gcttccggtt catacgatcc tgatggcgac aacataagtt acctgtggtt
      961 ctcttatccc gaagctggtt cctataaaaa gaagatttca ataaattcgg ccgaaaacat
     1021 gtataaagcc tgggttatcg ctcctgtggt cgacaaaaaa gaaaccgctc actttatttt
     1081 gaaagtaagc gacaaaggaa cccccagcct ttctagatat aaacgggtaa ttgttactat
     1141 attacctgag tgatagattg atggggtcat ggagccagcc gtgctgtttc ccagactccc
     1201 gaacaaagac gatacactat tcggtcgtga aggcgatgag ggccaccttg ccaaaactcg
     1261 aaataagaag ggttaagcat atacccgccc caattttcgg gtctaattaa gggttcgccc
     1321 gattcaagaa gatgttttcg ctgatcatca agaaatttgc gatcaggaat ttcatcactt
     1381 tgaggagaaa ttacagcact gacacgactt tcataagggc gggaagaaaa atactgatcg
     1441 gagattttca cgtcggtttt cgaaacttgc ccttcaaccc ggacttgccg ctcgagagaa
     1501 ggccagaaaa aacaagcaga aactttggga ttcaacgaaa tttcctgccc cttgcgactt
     1561 gagtaattgg taaaaaacac caggctctca tcggtgagga atttcaataa aacaaccctt
     1621 gaagaaggct gtccatcagt cccaacggtt gacaatatca tggctgaatg ttcttcacaa
     1681 tcagcatttt cagcctcggc gagccacaag gccaattgcc tcaacggatc ggtatcaacc
     1741 ccatcggcat cgagcctgtt ttttccataa ttgcgccgaa tatctgataa atccatatcg
     1801 atctgtttcc gaaatgcaac aaatattcat ttgaaaagtt tatttttgaa atgtcgacta
     1861 aaatgaaaaa aaacaaggca atgcaatcaa attcaaaatt cggaatcaaa gatattgtag
     1921 tcattcttct ggtaatcgtt ctggccgtaa tggtcgtatt atatttcacg aaacgcaacg
     1981 aacacaataa aatcgttagt gagctgaatg cagaaaaaga ttcaataaaa gttgaacttt
     2041 cgcaaatgct ggtcaactac gactcgctca aaaccaacaa cgagcaactg aacgaaaact
     2101 tgttgatgac ccaatcggaa ataaaaaacc ttttgaccga aatagagcaa gttaaacgcg
     2161 caagtgtaat cgaggtttct aattacaaag acaaagtgaa taccctgagg ggaattatga
     2221 aagacctata caaccaaatc gattcgctaa acgaaagaaa taaaatactt tacgccgaaa
     2281 acattgaggt aaaacaacgg tataccgcag ttcaatcaaa aaacgaacaa ctgaccaaag
     2341 aaaaagacga acttgcacaa actgtaaaaa tggctcaaat tcttgaagca ttgagcctta
     2401 gaattagcgg actcaacccc agggataaag aaaccgaccg catttcaaaa actcagaaaa
     2461 ttatggtaag cttcaccctg agcaagaacc ttactgccgt tcgtggagct aaaaacatat
     2521 atgtcagaat tatgcgcccc gaccagttac tgcttgttga gagccaaact gctcttttcg
     2581 aatttgaaga tttgaaaatc ccttattcgg ccaaacgcga agttaattac gaaggaaatg
     2641 aattgccagt gaatattttc tgggacaaca gcgggcacga acctcttatt cccggcacat
     2701 atactatcga tgtttttgcc gatgggtaca acattggaac aactaaatat ctgtttaaaa
     2761 actaattaac tactcaggat ggaaaatccg gtgaataata gcggtaacga aaccgatatc
     2821 tgttttcatg ggataaacct aaccggagca ttggttgcgc tggccgcatt gcacagtgga
     2881 tgtaaggttt cgatagaatt aaatcaaaag cctgatttac aatttcttcc cgaacttgtt
     2941 gcgctctacc ctatttcagc tttcgagctg ccaaattctc tttcgcggat taaacttctt
     3001 aaaaaaatcg caggatttta cccaacgtta gtttacccgc aacgcttgct cactctggtg
     3061 gaagataccc ggttgaaagc caggaaactt tctttcttcg atctgcttct cggtcgcgaa
     3121 cgcgaagatg cttctatgcc gatacgcttg tcgaaatacg atcaatacca atcaattgag
     3181 cccttttttg agaatggggc aatgataagc gagttccggt tcgacagaaa tatgttgttg
     3241 atc
//
LOCUS       DAOPUW010000155         3243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_30331_length_3243_cov_2.908720, whole genome shotgun sequence.
ACCESSION   DAOPUW010000155 DAOPUW010000000
VERSION     DAOPUW010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30331_length_3243_cov_2.908720"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..797)
                     /locus_tag="PKJ43_03655"
     CDS             complement(24..797)
                     /locus_tag="PKJ43_03655"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNZ71688.1"
                     /translation="MKYLIVLIAIICSTSLFAQDAKTIVEKNKTAMEANDQEATLTMK
                     LVGKSGSVRERSLVWSTMKDKNGLSSSYLLFIAPADIKGTAFLSIENASGNDDQWLFL
                     PALKRSRRISSNEKSQSFMGSDFTYDDLGEQDIDLSDYKLLCTETQGNMQLQVIESVY
                     KTPQKIKESGYSKRIIWIDAATFMLVKTEFYGIDGKLKKVLECSDFKQLGDKKLWRPQ
                     VYIMKNLEKGTETQLRFANYKIDQGIDASRFTLRNLESN"
     gene            complement(908..3187)
                     /locus_tag="PKJ43_03660"
     CDS             complement(908..3187)
                     /locus_tag="PKJ43_03660"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNZ71689.1"
                     /translation="MKSIINFILKRRNILLIIILLLTVGFSLKLGKVEMHEDEETFIS
                     PSDSVMIKYREFQKKFETDEGVVVAFESPNLFSKSEIQYLSSLHKKLEQLPGVTEVTS
                     LINADKVTGVADGIEINSIIDTTMLDDKLLMDAKLSIANDPMFEGVYLSSDHKVASII
                     VTIPGVFDGGTDSLNKAFYTELVALVNNEKDINNRDLHIGGDLVTDAAIGVLMDRDMA
                     VLFPISLLFCALVLFISFRKLVTTLVPLIPVLLAIVWVLGLKGWTGIPMTPISITLFP
                     LIMVIGLANSIHIISHYRKIRPQSPNSIEAMKETLSRVIKPSFMAAFTTAIGFGSLVV
                     SNVIGISQMGFFAAFGIMAAYFLSFIIIPSVLVSSNIFSNEKLVEKNSKAMDWTLHKI
                     NWLNQNHTWKIVLAFILVFVVMAMYIPRIKVEGSMASFAKEKTKLRNDIRFLDERLSG
                     INSYELIISGEENAFKNPEILRKIDKIEMELVQNPDVLKVFGVSNLVKIINKALNSDK
                     QEFFRVPETGQEVAQYLFLYEISGGDELSSFVNEDYSTARITIRTKQMSNEKQKVFIE
                     NLHQITSSQLGTLKTEVSGFGMLMSHINDNLIETQIESIIMALGIILALMFVMFGWKG
                     GFLSILPNIFPIVFFLGLMGIIGIDLNMATSIIASVTIGIVVDDTIHIFFRQREEMKE
                     LDSPLQAITTSLLKIGAAMCVTSLLLVIGFGILSISASKFIADFGLLSASAIAAALLA
                     DLFISPILLEKAKLFQKKN"
BASE COUNT          911 a          712 c          664 g          956 t
ORIGIN      
        1 gttcgtttca tggttcaatt tttctaattc gattcaaggt tgcgaagtgt aaagcggctg
       61 gcatcgatgc cctggtcaat tttgtaattg gcaaagcgca gttgggtttc agtacctttt
      121 tccagatttt tcataatgta cacctgtggt cgccacagct ttttatcccc caactgtttg
      181 aaatcggaac attcgagcac ttttttcagc ttgccatcga taccgtaaaa ttcggttttc
      241 accagcatga acgtggctgc atcaatccag atgatgcgtt ttgaataacc gctttccttt
      301 attttctgcg gagttttgta caccgattca attacctgca actgcatgtt tccttgggtt
      361 tcggtgcaca acaatttgta gtcagaaagg tcgatgtcct gttcgcccag atcgtcgtaa
      421 gtgaaatcgc tgcccatgaa actttggctt ttctcgttcg atgaaattcg tcgcgagcgt
      481 ttcagtgcgg gcaaaaagag ccactggtcg tcgttgccgc tggcgttttc gatggagaga
      541 aaagctgttc ctttaatgtc ggcaggtgcg atgaagagca agtacgacga tgacaagccg
      601 tttttgtcct tcatggtact ccaaacgagc gagcgttcgc gcacgctccc tgatttgccc
      661 accagtttca tggtgagcgt tgcttcctga tcgttggcct ccatggccgt tttgtttttt
      721 tcaactatgg ttttggcatc ctgtgcaaaa agcgatgtcg aacaaatgat tgcgattaaa
      781 acgattagat atttcatgat tttcagattt ttttttaccg cagagaacac agaggttttc
      841 tcagagatac acagagttga gttcgcgttt cgtgagaata ttcctttgtg cctttgcgtc
      901 tttgtgttta attcttcttt tgaaacaatt tagctttctc caataagatt ggcgaaatga
      961 acaggtcggc aagcaaagcc gctgcaatgg ccgaagccga gagtaacccg aaatcagcaa
     1021 taaacttact tgctgaaatg gaaaggatac caaacccaat aaccaaaagc agagaggtta
     1081 cgcacatggc cgcaccaatc tttaagagag atgtggtaat agcctgcaac ggcgaatcaa
     1141 gttctttcat ctcttcgcgt tgccggaaga aaatgtgaat ggtatcgtct accactatcc
     1201 caatagtaac cgaggcaatg atggaagtgg ccatattcag gtcgattcca attattccca
     1261 ttaaaccaag gaagaacaca atggggaaaa tattgggcaa tatgctcaaa aaaccgcctt
     1321 tccatccaaa catgacgaac ataagagcga ggatgattcc aagggccata ataatacttt
     1381 caatctgagt ttctatcagg ttgtcgttga tgtggctcat cagcattcca aaacccgaaa
     1441 cttcggtttt tagcgttccc aattgcgatg aagtgatttg atgaaggttt tcaataaaca
     1501 ccttctgctt ctcgttactc atctgtttgg tacgaatggt gatacgcgcc gttgaataat
     1561 cttcatttac aaacgacgaa agctcatcgc cacccgaaat ttcgtatagg aaaaggtatt
     1621 gcgccacttc ctgcccagtt tccggcactc tgaaaaactc ctgtttgtca ctattcaagg
     1681 ctttattgat gattttcacc agattggaaa ccccaaagac tttcaacaca tcgggatttt
     1741 gtacaagttc catttcaatt ttgtcgattt tcctcaatat ttcagggttt ttaaacgcat
     1801 tctcttctcc tgaaatgatc agttcgtagg aattgatacc cgaaagcctt tcatctaaaa
     1861 accggatatc gttgcgtagc ttggttttct ctttagcaaa cgaagccatc gatccttcta
     1921 ctttaattcg tggaatgtac atcgccatta caacgaaaac caaaataaaa gccaacacaa
     1981 ttttccatgt gtgattttgg tttaaccagt tgattttatg gagggtccaa tccatcgcct
     2041 tactattctt ctcaaccagc ttttcattgc tgaaaatgtt agatgaaacc aacaccgaag
     2101 ggatgataat gaacgacaaa aagtaggctg ccataatacc aaaggctgca aaaaatccca
     2161 tttggctaat cccgataacg ttgctgacca ccaacgatcc aaagccaata gctgttgtaa
     2221 aagcggccat aaaacttggt ttaatcaccc tgcttaatgt ctctttcatg gcttcgatgc
     2281 tattcggtga ttgcggcctt attttccggt agtggctgat aatgtggatg gaatttgcca
     2341 aaccaatcac cataatcagc ggaaaaaggg ttatcgaaat gggtgtcatc ggaatacccg
     2401 tccatccttt taagcccaaa acccatacga tggccagcag aaccgggatc aacggaacca
     2461 aggtggtgac tagtttcctg aaagaaatga acaataccaa ggcacaaaac aacaacgaaa
     2521 tagggaacaa caccgccatg tcacgatcca ttaacacacc tattgcggca tcggtaacca
     2581 ggtcaccacc aatgtgcagg tctcggttgt taatgtcctt ttcgttgttc accagcgcaa
     2641 ccaattcggt gtaaaatgct ttgtttaagc tatctgtacc cccatcaaac acaccgggaa
     2701 tggtaacaat aatcgaggcc accttatgat cggatgagag ataaactcct tcaaacatag
     2761 gatcgttggc tatagatagt tttgcatcca tcagtagttt atcgtcaagc atcgttgtgt
     2821 caataatcga attgatttcg ataccatcgg ccaccccggt aaccttatcg gcattgatga
     2881 gcgaggttac ttcggtgaca cctggtaatt gctccaattt tttgtgcaag gaggaaaggt
     2941 attgtatttc gcttttgcta aataggttgg gcgattcgaa cgcgacaact accccttcat
     3001 cggtttcaaa tttcttctga aactcacgat attttatcat tacagagtcc gacggtgaaa
     3061 tgaacgtttc ttcgtcttca tgcatttcga cttttcctag tttcaatgag aatccaacag
     3121 tcaggagcag aattattatc agtaagatgt tccttcgctt taaaataaag ttgataattg
     3181 atttcataat tagttagata aaactaaata tatttgtaag caaagatttc gacaatggac
     3241 gag
//
LOCUS       DAOPUW010000156         3233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_30466_length_3233_cov_2.767149, whole genome shotgun sequence.
ACCESSION   DAOPUW010000156 DAOPUW010000000
VERSION     DAOPUW010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30466_length_3233_cov_2.767149"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PKJ43_03665"
     CDS             <1..562
                     /locus_tag="PKJ43_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HNZ71690.1"
                     /translation="RPLSVFLCLIPFKTEVRNKNFISWVGLRGAVPIVFATYPLLAGA
                     EKASMIFNIVFFISLTSVLIQGTTLPFVSRLLKLSLPAEEKKPTQTDLHLFDSIKSML
                     TELLVPENCPTLGKQIVDLDLPPTTLISIIERGNEFFTPNGSTVIESGDKLFILAENN
                     ESLDEFLRRICIDKNGLATEPTDIAG"
     gene            747..2165
                     /gene="nhaD"
                     /locus_tag="PKJ43_03670"
     CDS             747..2165
                     /gene="nhaD"
                     /locus_tag="PKJ43_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HNZ71691.1"
                     /translation="MFELMVILFIVGYVFIALEHTVNINKAATALFMGISLWVVYILG
                     AESILGPSSPNFSEFINIHPQVAQLPIYEQFIHFIAENELLKYFAEVSEILFFLMGAM
                     TIVEIVDQHEGFSIITNRITTRKKVKLMWVIGIITFFMSSILDNLTTAIVMVALLRKI
                     IPDKHERWLMAGIIVIAANAGGAWSPIGDVTTIMLWINGNITSAGIIPSLFLPSLISL
                     AVPLVILSFTLKGKVEEHKIENTEKHKHKKVITDNERWLLLALGVAALLFVPVFKVTT
                     HLPPFMGMLLGLSVVWIFTEIMYHRKKDVDKNEKLTVSRVIKNIDVPTILFFLGILMA
                     VNALQSAGHLALLSSTLDNHLHNVYAINTIIGLLSSIIDNVPLVAAAMGMHTVATPEA
                     LAIATNPDYLQLFLQDGKFWTLLAYCAGTGGSILIIGSAAGVAIMGLEKMEFGWYLKK
                     ISLLAIAGYFAGIVCFALQNGF"
     gene            complement(2192..>3233)
                     /locus_tag="PKJ43_03675"
     CDS             complement(2192..>3233)
                     /locus_tag="PKJ43_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNZ71692.1"
                     /translation="SAPAMMGNVCIWKPSKNAVYSASVIMEVLMEAGIPDGVINMVFV
                     PGAVASEVLLSHRDFAGIHFTGSTPVFHGIWKKIGENIDRYKSYPRIVGETGGKDFIF
                     ADPTCDVTELVTAFVRGAFEFQGQKCSAVSRAYIPQSVWPIFWKETETILSHLKIGPP
                     EDFTNFINAVIDEDSFDKLKRYIDNAKTDSDVEMVYGGKCDKSIGYFIEPTVIVTKNP
                     GYVTMREELFGPIVTVYVYDDNQLDETLDMVDNTSPYALTGAVFSQNRNSISKISERL
                     VDCAGNFYINDKPTGAVVGQQPFGGGRASGTNDKAGSAFNLLRWVNIRTVKENFLPPK
                     NVLYPSFEPDVD"
BASE COUNT          949 a          724 c          609 g          951 t
ORIGIN      
        1 ccgcccgctg agcgtgtttc tctgcctcat tccgtttaaa acagaggtca gaaacaaaaa
       61 tttcatttct tgggttgggc ttcgcggggc tgtacccatt gtttttgcta cttacccctt
      121 gctagccgga gccgaaaaag caagcatgat tttcaatatc gtatttttta tctcgttaac
      181 ctcagttctt attcaaggaa caaccctgcc gtttgtatcg agacttctga aactttctct
      241 ccctgccgaa gaaaaaaaac ccacacaaac tgaccttcat ttattcgata gtattaaatc
      301 gatgctcacc gaacttttgg ttccagaaaa ctgcccgaca ttagggaaac aaatcgttga
      361 ccttgatttg ccacccacaa cactaatttc aatcatagag cggggcaatg aatttttcac
      421 acctaacgga tctacggtta ttgagtctgg cgacaagcta ttcatactgg cagaaaacaa
      481 tgaatccctc gacgaattct tacgacgcat ttgcatcgac aagaacggcc tggcaactga
      541 gccaactgat atagctggat gacaaaacaa taaatccctg aatcattaaa aaaaagttct
      601 catcaattga cattcaaaaa acagtcgtta agaaattata aagaacatga taaaaaaaat
      661 ataattatca ccttctttct cttataaatc tacttttgcg gccttaaaaa aaatcaagat
      721 ttattttcat ctaaaaagaa tttatcatgt tcgaattgat ggttatcctg tttattgttg
      781 ggtatgtttt cattgcatta gaacacacgg tcaacattaa taaagccgca acagcccttt
      841 tcatgggcat aagtttatgg gttgtataca ttcttggtgc agagagcatt ttaggtccct
      901 caagtccaaa tttcagcgaa ttcatcaaca tacatcctca agtagcacaa cttcctattt
      961 atgagcaatt cattcatttt atagcagaga atgagttatt gaaatatttt gccgaagttt
     1021 ctgagatttt gtttttcctg atgggggcca tgaccattgt tgaaattgtg gaccaacatg
     1081 aaggcttcag tataataacc aaccgcataa ctactcgaaa gaaagtcaaa ctcatgtggg
     1141 tcattggaat aataaccttt ttcatgtcat ctatcctcga caaccttacc actgcaattg
     1201 tcatggtagc cctactacga aaaattatac ccgataagca cgaacggtgg cttatggccg
     1261 ggataattgt tatagcggca aatgccggtg gtgcctggtc tccaattggc gacgttacca
     1321 cgataatgct ttggattaac ggcaacatta cttcagcagg aataattcca tcactgtttt
     1381 taccaagcct gatttcactt gccgtacctc tggtaatttt gtcgtttaca ctgaaaggaa
     1441 aagttgaaga acataaaata gaaaacaccg aaaaacacaa gcacaaaaag gttatcaccg
     1501 acaacgagcg atggttactt ttagctcttg gcgttgctgc attacttttt gtaccagttt
     1561 tcaaagtaac cacacattta cctccattta tgggcatgct gcttggtctt tcagtcgttt
     1621 ggatattcac cgaaataatg taccacagaa agaaggatgt cgacaagaat gaaaaactca
     1681 cggtctcgcg ggttattaaa aatatagatg ttcctaccat tttgttcttt ctaggaatac
     1741 ttatggctgt gaatgcgtta caaagtgccg ggcacctggc tcttttgtct tccactctcg
     1801 ataaccacct gcataatgtg tacgccatta acactataat tggcttactt tcatctatca
     1861 tcgacaatgt acccctggtt gctgcagcaa tgggtatgca taccgttgcc acacccgagg
     1921 ctcttgcaat agctaccaac cccgactatc ttcaattatt cttacaagat ggtaaatttt
     1981 ggacattgtt ggcatactgt gctggtaccg gtggaagtat cctcatcatt ggttctgcag
     2041 cgggtgttgc tattatggga ctcgaaaaaa tggagtttgg ctggtatctg aaaaaaatat
     2101 cattacttgc aattgctgga tattttgctg gaatagtatg ttttgcccta caaaacggtt
     2161 tttgaatcta tgtcaattta taccgaaaaa gtcaatcaac atctggctca aagctaggat
     2221 ataaaacatt tttgggcgga agaaaatttt ctttcacggt gcgtatattt acccaccgca
     2281 ggagattgaa cgccgaacca gccttatcat ttgtgcccga ggctcttcct cctccaaaag
     2341 gttgctggcc aactacagca ccggttggtt tatcgttgat gtaaaaattc ccggcacaat
     2401 cgactagcct ttcgcttatc ttggaaattg aattcctgtt ttgcgaaaaa actgctccgg
     2461 ttagggcata cggagaagtg ttgtcgacca tatccaatgt ttcatccaat tggttatcgt
     2521 cgtatacata aaccgtaaca atcgggccaa aaagttcctc gcgcatagtc acatagccgg
     2581 ggttcttcgt aacaatcact gttggttcaa tgaagtaccc tatgcttttg tcgcacttcc
     2641 ccccataaac catttcaaca tcagaatcag ttttagcgtt gtcgatatat ctttttagct
     2701 tatcaaacga atcttcgtcg atcacagcat tgatgaaatt ggtgaaatct tctggtggcc
     2761 caatttttaa atgagaaaga attgtttcgg tttccttcca aaagattggc caaaccgatt
     2821 gggggatata cgcccgggag actgccgagc atttttgccc ctggaattcg aaagccccgc
     2881 gcacaaaagc ggtaaccaac tccgtaacat cacatgtagg gtcggcaaaa atgaaatctt
     2941 ttccgccagt ctctcctact atgcgcggat agcttttata tcgatcaata ttctctccga
     3001 ttttcttcca aattccatga aagacaggcg ttgacccggt gaaatgtatc ccggcaaaat
     3061 cacggtgtga aagaagcact tcagaagcca cagccccagg aacaaaaacc atattgatta
     3121 ccccatcagg gattcctgct tccattaaca cttccatgat tacagatgca gaatacaccg
     3181 catttttaga gggcttccaa atacagacat ttcccatcat agccggagcc gac
//
LOCUS       DAOPUW010000157         3232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_30478_length_3232_cov_3.057602, whole genome shotgun sequence.
ACCESSION   DAOPUW010000157 DAOPUW010000000
VERSION     DAOPUW010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30478_length_3232_cov_3.057602"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            452..>3232
                     /locus_tag="PKJ43_03680"
     CDS             452..>3232
                     /locus_tag="PKJ43_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNZ71693.1"
                     /translation="MSLQKKQLRVLLKAIMASFLFFMVIQSQAALQEKKTITGSVKDA
                     ETNEPLPGATILEKGTTNGTITDVDGNFTLQVKANGSLQISYVSYKTEEVAITGSSQY
                     TISLSPDVVGVSDVVVIGYGTVKKSDLTGAVSSISSEDIRQNIGSGIDQALQGRTAGV
                     TVTANSGAPGAAPTVRIRGMGTITNPNPFYVIDGIPVSSESVGMLNPGDIESMEVLKD
                     ASAAAIYGARAANGVVLITTRRAKAGKSAISVDAYTGVQSVAKKYDVMTAKDWVTIRN
                     AAGQPWMDSSTVQQTDWQDELFRQAKVNSVQLSLLNGNENISSAIIGSYFKQDGIVKG
                     SGYERFTVRVNTASKLKPWLTVGENIGYSHAKQNLIPEQNEWSSAVVQALVMDPTTPV
                     YDANGNHAGAILNNITNPVGVAERSHNILKTQQLLGNVYLEIKPVSWLTFKSSVSTEI
                     NDYDNEQFFPIFTESSTVQSNVTTLAKGNYKLNTLLTEQLLTFQKTLFEKHDVQFLLG
                     YTRQQTSYRLNVSAVSGVPEASDLWMIANGDASTLITGDVVGASSPLSAYVPSSLSGI
                     PYDASMVSYLARLIYSYNNRYDLTASIRRDGSSKFGSAKRWGNFESFALGWKLSEEAF
                     FPKNDIVNFAKIRFGWGELGNQEIGDYAAYTNVTYGFDATFGEVGSQERLPGGAPTGF
                     ANSGIQWETTVQTNIGLDANLFKSKMSLNFDYFTRLTTDMLAQVPVPGLTGIQVAPTA
                     NAGSVSNKGWELNVSYRERTGVFKYELGFNIGQVKNNVEKLGSNQPINSASFRAIDFI
                     SRTEVGMPIAYYYGYICDGLYQNTDEIEKYNQMAREATGKPKQVYDNKKAMPGDIIYK
                     DISGPEGKPDGIITSADRKFIGSPHPDLTYGVNLKFEYKNFDLSVFGQGVYGNEVFMA
                     TTFYLMSGDAYWN"
BASE COUNT         1021 a          678 c          678 g          855 t
ORIGIN      
        1 aagcaatctg ttttgtgttt ttaaattttt accggacaac aatgattagg tctataatta
       61 attgtgcaaa atcaaaaata cgccaccatc cctaccctcc aaaaagaatt tgatatgacc
      121 cattccaaat caacttcgta acatataggc ttacaaaacc gaatagacgc tgagtgtatt
      181 cgaataaatg aagcacactc tacagaaaac agatcgtcga gaaggtgtta tattcacact
      241 attaaaaata tttacttcat cgggagatca aaactcaaaa aaaaagaaaa gaaaactttt
      301 aaatttcaag acgatatata aataaattta ttatttttcg gtgattttta taaagcgtaa
      361 aaattacact ttataaaaat tcaagatatt tttttacaat ctaaaaacct tgtagttaat
      421 tattaaccta aaaagtatga aaaatcatgt aatgagccta caaaagaaac aattgagagt
      481 tcttctcaag gcaattatgg catcgttcct gttcttcatg gttattcaat ctcaggcagc
      541 cctgcaggag aagaaaacca tcacggggag cgtgaaagat gctgaaacta acgagcccct
      601 tcccggagca acaattttgg aaaaaggcac caccaatggt accattaccg atgttgacgg
      661 gaacttcacc ttgcaggtga aagcaaacgg cagccttcaa atttcgtatg tttcttataa
      721 aacagaagaa gttgcaatta ccggcagcag ccagtatact atttcattaa gccccgacgt
      781 agtgggcgta tccgatgttg tggttattgg atacggtacg gtgaagaaaa gcgacttaac
      841 cggagcggta tcttcaatat cgtcagaaga tatccggcag aatattggat caggtatcga
      901 ccaggcactc caaggacgta ctgccggcgt aacggtaacg gccaatagtg gtgccccggg
      961 tgctgctcct accgttcgaa tccgcggtat gggtaccatt accaatccga acccattcta
     1021 tgttatcgac ggtatccctg tatcttcaga atcggtgggt atgcttaacc ccggagacat
     1081 tgaatcgatg gaagtactca aagatgcctc tgcagcagct atttatggtg cccgtgcagc
     1141 caatggtgta gtgcttatta ctacccgccg ggcaaaagcc ggaaaatcag caatctcggt
     1201 cgacgcatat actggtgttc aatcagttgc taagaaatat gatgtaatga ccgctaaaga
     1261 ttgggtaact atccgtaacg ctgcaggcca accctggatg gacagttcta ctgttcaaca
     1321 aaccgattgg caagatgaat tgttccgtca ggcaaaagtt aacagtgtgc aactttcatt
     1381 actcaatgga aatgaaaaca ttagtagcgc tataatagga agttacttca aacaagacgg
     1441 tattgtgaaa ggctcaggct acgaacgttt taccgtgcgg gtaaatacag catcgaaact
     1501 gaaaccgtgg cttactgtag gtgaaaacat agggtacagc catgcaaaac agaacttaat
     1561 tcccgagcaa aatgagtgga gttctgcagt tgtgcaggct ctcgttatgg atccaacaac
     1621 tccggtatat gatgccaatg gaaaccatgc aggagcaata ctcaacaaca ttactaaccc
     1681 tgtaggtgtg gctgaaagaa gtcataatat tctgaaaaca cagcaattgc ttggaaatgt
     1741 ttaccttgaa ataaaaccag tttcatggtt gacctttaaa tcaagtgtca gcactgaaat
     1801 taacgattac gacaacgaac aattcttccc gatattcaca gaatcgagta ctgtacaaag
     1861 caacgttacc actcttgcca aaggaaatta caagctgaat accttactta cagaacagtt
     1921 acttacattc cagaaaacct tgttcgaaaa acatgacgtt cagtttttgc tcgggtacac
     1981 ccgtcagcaa acttcttacc ggttgaatgt aagcgcagtt tcgggggtac ccgaggccag
     2041 tgacctttgg atgattgcta acggcgatgc gagtacttta attacaggag acgttgtagg
     2101 agcatcgagc ccattatcag cttatgtacc atctagcctc agtggtatac cttatgatgc
     2161 ctcaatggtt tcttacctcg cccggttgat ctactcatac aataaccggt acgaccttac
     2221 tgcttcgatc cgccgcgatg gctcgagtaa attcggttcg gcaaaacgtt ggggtaattt
     2281 cgaatctttt gccttaggat ggaaactttc ggaagaagca ttcttcccca aaaacgacat
     2341 tgtgaatttt gcaaaaatcc gttttggatg gggagaactt ggaaaccagg aaattggtga
     2401 ctatgcagct tacacaaatg taacgtatgg tttcgatgcc actttcggtg aagttggttc
     2461 tcaggaacgt ctgcccggtg gtgcacctac aggctttgcc aacagcggga ttcagtggga
     2521 aactactgtt cagaccaaca ttggtttaga tgccaacctt ttcaaatcga aaatgagctt
     2581 aaacttcgac tactttaccc gcttaaccac cgatatgtta gcacaagttc ctgttcccgg
     2641 attgactggt atccaagtgg caccaacagc caatgcagga tcagtttcta acaaaggttg
     2701 ggaacttaac gtatcgtacc gtgaacgtac cggtgtgttt aaatacgagt tgggtttcaa
     2761 cattggtcaa gttaaaaaca atgttgaaaa attaggtagc aatcagccaa tcaattcagc
     2821 ctccttccgt gctatcgatt tcatttcgag aactgaagtg ggaatgccta ttgcttatta
     2881 ttatggttac atttgtgatg gattgtatca aaacactgat gaaattgaaa aatacaatca
     2941 aatggcccgt gaagctaccg gcaaacctaa acaagtgtac gacaacaaaa aagccatgcc
     3001 cggcgacatt atctacaaag atattagtgg accagaaggc aaacccgacg gcattattac
     3061 cagtgctgac cgtaagttta ttggaagccc acacccagat cttacatatg gggtaaacct
     3121 gaaatttgaa tataagaact tcgacttgtc agtgtttggt caaggagtat acggaaacga
     3181 agtatttatg gccaccactt tctacctgat gagtggcgac gcatactgga at
//
LOCUS       DAOPUW010000158         3226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_30542_length_3226_cov_3.573636, whole genome shotgun sequence.
ACCESSION   DAOPUW010000158 DAOPUW010000000
VERSION     DAOPUW010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30542_length_3226_cov_3.573636"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..488
                     /locus_tag="PKJ43_03685"
     CDS             207..488
                     /locus_tag="PKJ43_03685"
                     /inference="COORDINATES: protein motif:HMM:NF018563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HNZ71694.1"
                     /translation="MFKVNRYFDDCVMSMAFENEEGRATVGIMAAGEYEFSTSSIEYM
                     TVISGKMSVLLPGETKWKDFKPYDTFVVGKDLSFKLVIEADCAYKCLYK"
     gene            complement(507..1103)
                     /gene="ruvC"
                     /locus_tag="PKJ43_03690"
     CDS             complement(507..1103)
                     /gene="ruvC"
                     /locus_tag="PKJ43_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNZ71695.1"
                     /translation="MNSKRVVLGIDPGSNVMGYGIIQTEGKQSGIVAMGTISPGKITD
                     HYAKLKYIFDRVLHVIDEYKPTEVALEAPFFGKNVQSMLKLGRAQGIAMAAAMYHNLP
                     IFEYAPLKVKQSITGMGQASKEQVAYFLNKIYGIEIPHLLDATDAVAVAYCHILQSSR
                     PALTKEFRNWEDFIRKNPDKVIAPNPRKSNQNKKAPDK"
     gene            complement(1103..2404)
                     /locus_tag="PKJ43_03695"
     CDS             complement(1103..2404)
                     /locus_tag="PKJ43_03695"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNZ71696.1"
                     /translation="MKNCISRKKTNNGKIPLLLIVFIFTTTFLSTSCSSFRHEEDKTA
                     NEYIYETFQDWYLWNNKLPIVDPNTFETGQELIDSICVEQDRWSFAGSLTSVKQLFEA
                     GEFTGFGGNMMLDAQKRIWVGLVYRNSPMGRIGVQRGWEIKSIDGFTTEYLEEINNAL
                     AKEQSVTFEFVDNNGETHTATLKREVVTMNTVLHSSIIEEGPHKIGYLVFDSFVETSE
                     AELDSVFTRFNQEKITDLIVDLRYNGGGLNDIAYKLTAMIGGSKVKNKLITLTQHNSN
                     HFNYNLEKIADYKGTSVEVPTVHFITTKETASASEVVINNLIPYMEVVIVGRATHGKP
                     VGMYIFEVEKLDLAILPICFKTTNKDGNGDYFNGLQPTLEVADDITRPWGDPEEGMLK
                     ATVAFIANPVLATSAFPLKSQPISTLEPIKYQGINKLIGAY"
     gene            complement(2459..>3226)
                     /locus_tag="PKJ43_03700"
     CDS             complement(2459..>3226)
                     /locus_tag="PKJ43_03700"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ71697.1"
                     /translation="AVELPELGNLQRSALKLELADLNVYDNSLWTATLLYAQIIDSNK
                     DNPIGDEAKLKKAKLGFYLGDINWAKAQLDVLKASTSKLIANDAMELSLLITSYYELD
                     TVELPIQIFARGDLLVLRNKDNEAVETYDSITNLFPGHSLQDKILMRKAQISEKNFRF
                     EEAVGFYKQLLANFASSTSADDALYNMAVIMEEKLGQKEQASELFKQMLTTYPSSIFT
                     ADSRQRYRKFRGDLPEEEDPATEAEKERMLNETFRPE"
BASE COUNT          777 a          645 c          749 g         1055 t
ORIGIN      
        1 cgtttcgtca gaaaagggag gggcaaatgt tgctcatggc tgggtatttt ttcctgcttt
       61 tgtctttttt gtgttgtgtt ttttattcta tttcgaatag gagtgatttg caaaagttaa
      121 tattttttca attatgtcga ctgtagtgtt tttattgcgt cttttttgta taataatggt
      181 tagaattgtc gaaaaaggac atcaatatgt ttaaagtgaa tcgatatttc gatgattgcg
      241 taatgagcat ggcattcgaa aatgaagaag gcagggctac agtaggtata atggcagctg
      301 gtgagtatga attttcaaca tcatcgattg agtatatgac cgttatctcg ggaaagatga
      361 gtgttcttct tcccggtgaa accaaatgga aagattttaa gccgtacgat acgtttgtgg
      421 tagggaaaga cctgtcgttc aaattggtaa ttgaggccga ttgcgcgtac aaatgtcttt
      481 ataaataaac cctttgcact aaatgtttat ttatcagggg cttttttgtt ttggttggat
      541 ttccgtggat tgggagctat cactttgtca gggtttttcc gtataaaatc ttcccaattt
      601 ctgaattcct tggtcagagc tggccggctc gattgaagaa tatggcagta tgcaacggct
      661 acagcatcgg ttgcatcgag cagatggggt atttcgatgc cataaatttt attcagaaaa
      721 taggcgacct gctctttcga agcttgcccc ataccggtaa ttgactgttt tactttcaac
      781 ggagcgtatt caaaaatggg aaggttgtga tacatggctg ctgccatggc tattccctgg
      841 gcgcgcccaa gtttcagcat cgattgcaca tttttgccaa agaacggcgc ttcgagggca
      901 acttcggtgg gtttgtattc atcgattaca tgaagcaccc tgtcgaaaat atatttcaat
      961 ttggcgtagt gatctgttat tttacccggc gaaatagttc ccatggctac aatgcccgat
     1021 tgtttccctt cggtttgaat tatcccgtaa cccattacgt tcgatcccgg gtcgatgccc
     1081 aaaactactc gcttgctgtt cattagtagg ctcctattaa tttgttgatc ccttggtatt
     1141 ttatgggttc caaagtgctt atcggttggc ttttaagagg gaaagcacta gtagccaata
     1201 cggggttggc aataaacgct accgttgctt taagcattcc ttcttcgggg tcgccccaag
     1261 gccgggtaat gtcgtcggcc acttcaagtg ttggctgaag cccgttgaaa tagtcaccgt
     1321 ttccatcttt attggtggtt ttgaagcata taggcaaaat ggccaaatct agcttctcta
     1381 cttcaaaaat atacattccc accggtttgc cgtgcgtggc tctgccaaca atcactactt
     1441 ccatgtatgg gattaggttg ttgatgacta cttcgctggc cgaggctgtt tcttttgtgg
     1501 taataaagtg cactgttggc acttcaactg aagttccttt gtaatcggct atcttttcta
     1561 aattataatt gaaatggttg ctgttgtgtt gggtcagtgt aatgagcttg tttttcactt
     1621 tgcttccgcc gatcatggct gtcagtttgt acgcgatatc gttcagcccg cccccattat
     1681 aacgtaagtc aacaattaaa tcggtaattt tttcctgatt gaaacgggtg aaaaccgagt
     1741 cgagttcggc ttctgaggtt tctacaaaac tatcgaaaac cagatagcca attttatgag
     1801 gcccttcttc gattattgat gaatgcagca cggtattcat ggtcaccact tcgcgcttaa
     1861 gggttgccgt gtgggtttcc ccgttattgt cgacaaattc gaaggttact gattgttctt
     1921 tggccagtgc attgtttatt tcttccagat attcggtagt gaacccgtcg atacttttta
     1981 tttcccagcc ccgctgtacg ccaatccgac ccattgggga gttgcggtac accagtccaa
     2041 cccaaatacg tttttgggca tcaagcatca tgttgccgcc aaatccagta aattcacctg
     2101 cctcaaaaag ttgcttaacg ctggtcaacg atcctgcaaa gctccatcgg tcttgttcta
     2161 cacagataga atcgatgagt tcctgacctg tttcgaatgt attcggatca actatgggga
     2221 gtttattgtt ccacaaatac caatcctgaa aggtttcgta gatatattcg ttggctgttt
     2281 tatcttcttc gtggcgaaat gacgagcaag aggttgaaag aaaggttgtt gtaaaaataa
     2341 atacgatgag cagtaagggt atcttgccgt tattggtttt ttttcgggat atacaatttt
     2401 tcattttctg ttccgggtta gtacaggtaa taaattttgt agtatcgcac tactggtttc
     2461 attcgggcct gaacgtttca ttcagcattc gttctttttc ggcttcagta gccggatctt
     2521 cttcttcggg caagtcgccg cgaaatttcc ggtaacgttg gcgcgagtcg gcagtaaaaa
     2581 tactcgacgg gtaggtggtg agcatttgtt tgaaaagttc gctggcttgc tctttttgcc
     2641 cgagcttttc ttccataatt acagccatgt tgtataaagc atcgtcggcc gaggttgagg
     2701 aagcaaaatt ggcaagcagt tgtttgtaaa agccaacagc ctcttcgaaa cggaagtttt
     2761 tttcgcttat ttgggctttt cgcattagga ttttatcttg aagcgagtgc ccggggaaaa
     2821 gattggtgat ggagtcgtaa gtttcaacgg cttcattgtc tttgtttcta agaaccaaca
     2881 gatcgccgcg tgcaaaaatc tgtattggca attctacggt gtcgagttca taatacgaag
     2941 tgataagcaa agaaagctcc atggcatcgt tggctatcaa tttcgaggta cttgccttta
     3001 gcacatcgag ttgagccttg gcccagttaa tgtcgccaag atagaaaccc aatttcgctt
     3061 ttttgagttt agcttcatct ccaatgggat tatctttgtt actgtcaatt atttgagcat
     3121 acagcaaagt ggctgtccac aggctattgt cgtacacgtt taggtcggcg agttcaagtt
     3181 tcaacgccga acgctggagg ttgcccaact ctggcagttc gacggc
//
LOCUS       DAOPUW010000159         3219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_30638_length_3219_cov_4.051833, whole genome shotgun sequence.
ACCESSION   DAOPUW010000159 DAOPUW010000000
VERSION     DAOPUW010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30638_length_3219_cov_4.051833"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PKJ43_03705"
     CDS             <1..588
                     /locus_tag="PKJ43_03705"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNZ71698.1"
                     /translation="AFLQHIIAMLDEHGRAAVVLPDNVLFEGGAGETIRKRMLDELNL
                     HTILRLPTGIFYAQGVKANVLFFEKGTENKSLKTKEVWYYDYRTNIRHTPKKNPLRYE
                     HLEDFVECFKPDNKEQRKETFHSKENPEGRWRKYTYDDLVARDKTNLDITWLKDENLI
                     DLENLPEPETLIDEIIDNIQSALESFKTIKESLEI"
     gene            1009..1848
                     /locus_tag="PKJ43_03710"
     CDS             1009..1848
                     /locus_tag="PKJ43_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HNZ71699.1"
                     /translation="MGNIKLFQNQKVRTYWDKTEQQWYFSLVDVVEILTDSKNPTDYL
                     KKVRKRDDELNKYIGTNCPQVEMITSTGKIRKTLAGNAEHLFRVIQSIPSPKAEPFKQ
                     WLAKVGYERVQEIENPELAQERMKLIYEQKGYPSDWIDKRLRGIAIRQNLTDEWKERG
                     IQSDNDYAILTAEISKATFGLTPTEYKDLKGLTKKSQNLRDHMTDLELIFTMLGERVT
                     TEISKKEKPGTFPENKLVARRGGKVAGVAREETEKELGRSIISDQNFLEHTENDEPLP
                     LTE"
     gene            2373..2897
                     /locus_tag="PKJ43_03715"
     CDS             2373..2897
                     /locus_tag="PKJ43_03715"
                     /inference="COORDINATES: protein motif:HMM:NF017913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HNZ71700.1"
                     /translation="MIKTIHFIERTIIYTLIVMMAGVLVLATIELGYFLVKSILQSSF
                     LFIELDNLMDLFGVFLLVLIGIELLDTIKVYLRQNVVHVEVVILVAIIAIARKVVVLK
                     IEELESMMIIGIGVLVVSLAVAYYLIKRAGLIMLDCDKVLNSTGKDVNKVRIETDIKE
                     QGAEEAANSNDLLK"
     gene            3012..>3219
                     /locus_tag="PKJ43_03720"
     CDS             3012..>3219
                     /locus_tag="PKJ43_03720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ71701.1"
                     /translation="MVICVFNEEDNIAPLANRIVEALSGYDFEAIFVDDGSTDRTKEK
                     IRAIADNRFLLVELRKNFGQSSALQ"
BASE COUNT          960 a          608 c          697 g          954 t
ORIGIN      
        1 gcttttttgc agcacatcat tgctatgctc gatgagcatg gccgtgccgc ggttgtactg
       61 cccgacaacg tgctgttcga agggggtgct ggggagacca ttcgcaaacg aatgttggat
      121 gagttaaacc ttcataccat tttgcgcctt cctacgggta ttttctacgc tcagggagtg
      181 aaagccaatg tgttgttttt cgagaaaggc actgaaaata aatcattaaa gacaaaggag
      241 gtttggtatt acgattaccg cacaaacatc aggcatacac caaagaagaa tccgttaaga
      301 tatgagcact tagaagactt tgttgaatgt tttaaacctg acaataaaga gcagagaaaa
      361 gagacgtttc actcaaaaga aaacccggaa ggacgctgga gaaaatacac ctacgacgat
      421 ctggtcgccc gcgataaaac caatctggac attacgtggc taaaggatga aaacctgatc
      481 gatctcgaaa atctgccaga accagaaaca ttgattgatg aaattatcga caacatccag
      541 agtgcattag aaagttttaa gacaataaaa gaaagcttgg aaatataggg taagcaaaac
      601 catttttgct atgccaaggt cacaccttct tttctgcctg caggaactat agacatgcca
      661 acccacagtg tttggtgcat atatacatgc aattgaattt gtaaaatttg tggtttaatt
      721 tattatccta cctgtttcgt ccgaatggag ttttgttctc tgaataacat tctaccgcgg
      781 acgatgcacc tggtcgaaca ttttcccccg ggataaatcc cggggctatt gatgtttaac
      841 ccgctggggt gttttctctg tggaactctg tgcctcctcc gtgttctctg tgttaaaatt
      901 tatcggaact ctgtggttac ttcgttttat aatttcatca atattcgttc tcactgttta
      961 ccccatattt ttgtaaattg catcgcaaaa cccaaaaatt aatcggaaat gggaaatata
     1021 aaactctttc aaaaccaaaa ggtaagaacc tactgggaca aaaccgaaca acaatggtat
     1081 ttctcgttgg ttgatgttgt tgaaatactg actgattcaa agaatcctac tgactattta
     1141 aagaaagtca ggaaaaggga tgatgaacta aataaataca tagggacaaa ttgtccccag
     1201 gtagaaatga taacttccac cgggaaaata cgaaaaacac ttgccggaaa tgcagagcac
     1261 ttgtttcgtg tcattcaatc cataccttca cccaaggcag aacctttcaa acaatggtta
     1321 gcgaaagtcg gttacgaacg tgtacaggag atagaaaatc ccgagcttgc acaggagcgt
     1381 atgaaactga tctacgaaca gaaaggatat cccagcgatt ggattgacaa acggcttcgt
     1441 ggcattgcaa ttcgtcaaaa ccttaccgat gaatggaaag aacggggaat tcaatctgac
     1501 aacgattatg ctatcttaac agcagagatt tcaaaagcta ctttcgggct taccccaact
     1561 gaatataagg acttgaaagg tctgaccaaa aaatcgcaaa accttcgcga tcacatgacc
     1621 gatctggaat taatttttac catgctgggt gaaagggtaa ccactgaaat ttcgaaaaaa
     1681 gagaagcccg gaaccttccc cgaaaataaa ttggttgccc gcaggggtgg taaagtagcc
     1741 ggtgtagccc gtgaagaaac cgagaaagaa ttggggagga gtatcatctc cgatcaaaat
     1801 tttctggaac atactgaaaa tgacgagcca cttcccctta cagaatgatt taaggttgga
     1861 tgcagatata cgtgcaattg aatttgtgaa atttgtggtt atgatttatt atcctatcag
     1921 tttcgtccga aggaagattg gcactttaaa gggcattcta ccgcggacga tgcatctggt
     1981 cgaacattta ccccccccgg gataaatccc ggggctattg atgttgtagc ccgttgcagg
     2041 gctttttttt taaaagtgct tttaagataa atcccggggc tattgatatt tcgccccgct
     2101 ggggtgtttt ctctgtggaa ctctgtgcct cctccgtgtt ctctgtgtta caatttctcc
     2161 ttgtaattag ttccatattg tagttgaaag ctcatgcgtt actttttttt ggattgaatt
     2221 tcataatttt aaatcaaaat tgaatagggt ttttgttttt attgtctaat aaagctcgtt
     2281 cactaaagac ggtttcaaca aaaaggtaag cgctgtgttt atttaatcgt attatattca
     2341 ctaaattgct aaaacgtaaa atatttgcaa ctatgataaa aacaattcac ttcatcgaaa
     2401 ggacaatcat ttacactctt attgtaatga tggcgggggt tctggtgttg gctaccatcg
     2461 aactgggcta tttcttggtg aaaagcatcc tccaatcttc tttcctgttt atcgaactcg
     2521 acaatttgat ggatcttttc ggggtttttc tccttgttct tattggaatt gagttgcttg
     2581 atactattaa agtgtatttg cgccaaaatg tggttcatgt ggaggtggtg atattggttg
     2641 ccattattgc cattgcccgg aaagttgtgg tgctgaaaat agaagagctt gaaagcatga
     2701 tgattatcgg tattggagtg cttgttgttt ctctggctgt cgcttactac ttaataaaac
     2761 gtgctgggtt aattatgctc gactgcgata aggtacttaa ttccaccggg aaggatgtga
     2821 acaaagtacg tatagagacc gacatcaagg agcaaggagc tgaagaagcg gcaaattcca
     2881 acgacctatt aaaataataa gattggcaga tgaagttatt tcgtaacttt ttttccaaat
     2941 ttttgcctga gtatcatttt atcgtaagtt tgaattttgt ttcataattc ttatttgtaa
     3001 tgcggattag cttggttata tgcgttttca acgaagagga taacattgcc cccttggcca
     3061 atcgtatcgt agaagccttg tcggggtacg attttgaagc cattttcgtt gatgatggat
     3121 cgaccgatcg gaccaaagag aaaataaggg ctattgccga taaccgtttt ctgttggtcg
     3181 aattacgtaa gaattttggg caaagctctg cattacagg
//
LOCUS       DAOPUW010000160         3216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_30675_length_3216_cov_3.636507, whole genome shotgun sequence.
ACCESSION   DAOPUW010000160 DAOPUW010000000
VERSION     DAOPUW010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30675_length_3216_cov_3.636507"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2841)
                     /locus_tag="PKJ43_03725"
     CDS             complement(<1..2841)
                     /locus_tag="PKJ43_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNZ71702.1"
                     /translation="MMKRVMLLIVFVIISTVLYPKNSGGLKTNDTQKISSQNLSKILS
                     GKLIETMMFSKKSTSGNLKNALISSCRVILYFDAYPSASQIKELEDQEVSLYFNTWTP
                     PVESHRYGFIVASVPTANIKKVIELPFVKKIDWGERSSRAMNNSASLSINAQMVWTKG
                     YKGKGKKICILDSGIDISYAGSDFPSSFEYKDYSYFPEVDDNVANTITGHGTHVAATA
                     LGRGVLSEGQNHWNNGKGAFKGMAPDADLVFLKIGQDEDAFAEDAPIIAAIDAAVNVY
                     HADIISMSYGGWDDYHDGSSAVEQKVDWAYSQGVPFFCSAGNDANMEKHWRGTVAANS
                     QSDFIEVNVNDPAGEKTMLRFNMVWADNIQRSDLSLQYFDSLKQPLGDVTILPATESI
                     KGTESCYSWYNQELTSAGTFYVKVVNNSNQAREVHIFEDWNNQRNGSDRVYFSNADPS
                     FTIVSPASALHAFAVGAYVSKTGWTNPEDHSRWWGPSSVLNNIAPFSSLGPTLDQRIK
                     PDICAPGAVVLSLRDKDVYKTLNNSWIDNDGLPGGDANYYSMRGTSMACPVVAGAAAL
                     FLEKHPDASPQQVYDAMKNFSNESGLSNLPNNTWGYGKLDINNAIQNWRNKILVDGDM
                     ADDRYKTLAAFTSGQNGFGTKNNLGELKYHTDGENLFIGITGEVTSNDNILLFFDFSG
                     VQGRGSSTLGGGNSGDFVYCSFAYMGNVQMDFDVDFALGFNEGNSTQFEFFTDAIRYG
                     TSNKAANIGKTNQMGGSSNFEIGSVFGGSGFITVAYDSSFSVNRQKGVEIKIPISAFA
                     GVDTSQALRLFAVISSMEGNVSNECIPGNPGANNLGNGADFASIPNQDFFTQPVKISA
                     PQATTFVLSNEDGLIDDLIVGGYGDGSLYDALIALEPASGGIISQSFLDVLSNCPAGS
                     QIFTKKLNEYAHLKGGICINTDGTVFTTIDIAG"
     gene            complement(2875..>3216)
                     /locus_tag="PKJ43_03730"
     CDS             complement(2875..>3216)
                     /locus_tag="PKJ43_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ71703.1"
                     /translation="NPNDDAYRVATVTVAATGAASQSVEVVQDVTVGVSENALNDKSV
                     YPNPFTSGFYVNPDGINTTVLIYDLRGREIYTRIISKTEYFSFDGMLNGVYLIELKSG
                     QTTFSKKLIKR"
BASE COUNT          921 a          743 c          612 g          940 t
ORIGIN      
        1 cccagcaata tcgatggtgg taaacaccgt tccatcggtg ttgatacaga ttcccccttt
       61 gaggtgggca tattcattca gttttttggt aaatatctgg cttccggcgg ggcaatttga
      121 taagacatca aggaagctct gtgatataat gcctcccgaa gctggttcca acgcgatcaa
      181 agcatcatac aaactgccat ctccgtatcc tcccacaatc aaatcatcaa tcagtccatc
      241 ttcattacta agtacaaacg tagtagcttg tggcgctgat attttaaccg gttgggtgaa
      301 gaaatcctga ttgggaatgg aagcaaagtc agcaccgttg cccaggttgt tcgcacccgg
      361 attgcctgga atacattcgt tcgaaacatt accttccatc gatgaaatta cagcgaacaa
      421 acgcagggct tgcgaagtat cgaccccggc aaaagccgaa attggaattt taatctcaac
      481 acctttttgt cgattaacag aaaagctact gtcataggct acggtaataa aaccggatcc
      541 accgaaaaca gaaccaattt caaagtttga actccctccc atttggttgg ttttcccaat
      601 attagctgct ttattcgaag tcccataacg gattgcatcg gtgaaaaatt cgaattgagt
      661 actattgcct tcgttaaaac ccagggcaaa atcgacatca aaatccattt gtacattacc
      721 catatacgca aatgaacaat aaacaaaatc acccgaattc ccgccaccta gtgtactgga
      781 tcctcgtccc tgaactcctg aaaaatcaaa gaaaagcaga atgttatcgt tgctggttac
      841 ctcaccggtg attcctataa aaaggttttc gccatcggta tggtatttca attcaccaag
      901 gttgttttta gttccaaacc cattttgtcc gctcgtaaat gcagccagcg ttttatagcg
      961 atcatcggcc atatcaccat cgaccaaaat tttattgcgc cagttttgta ttgcgttatt
     1021 gatatccagt ttgccatatc cccaagtatt attaggaaga ttgcttaaac ccgattcgtt
     1081 actgaagttt ttcatcgcat cataaacctg ttggggtgaa gcatcggggt gtttttcaag
     1141 aaaaagcgct gcagctccgg caacaaccgg gcaagccata ctggtacctc tcatggaata
     1201 ataattggca tcgccacctg gaagtccatc attatcgatc caggaattat tgagggtttt
     1261 gtatacatcc ttatcacgca acgataaaac gactgctccc ggcgcacaaa tatctggttt
     1321 tatacgctga tccaaagttg gtcccaaact cgaaaagggc gctatattat ttaaaaccga
     1381 agaaggcccc caccaccggg aatgatcttc ggggttagtc cagccggttt tcgagacata
     1441 agctccaact gcaaaagcat gaagcgccga ggccggtgac actatggtga acgatgggtc
     1501 ggcattggag aaataaaccc gatcggaacc attacgctgg ttgttccaat cttcaaagat
     1561 atgaacctca cgggcctgat tagagttatt aacaactttt acataaaaag ttcctgccga
     1621 agtaagttct tgattatacc atgaatagca cgattctgtg ccttttatgc tttcggtggc
     1681 cggtaaaatg gtaacatcgc ctaacggctg ttttaatgag tcgaaatatt gtagcgacag
     1741 atcactcctt tgtatattat cggcccaaac catattaaac cgtagcattg ttttttcacc
     1801 agccggatca ttaacattta cttcaataaa gtcgctttgt gaattggcag ccactgttcc
     1861 cctccagtgt ttttccatat tggcatcgtt acccgctgaa caaaaaaacg gaactccctg
     1921 actgtatgcc caatcgacct tttgttccac ggcagaacta ccatcatggt aatcgtccca
     1981 accaccataa ctcatcgaga tgatgtcggc atgataaaca tttacggctg catcgattgc
     2041 tgcaatgatt ggtgcatctt cagcaaaggc atcttcatcc tgcccgattt ttaaaaaaac
     2101 cagatcggca tcgggagcca ttcctttgaa tgcccctttc ccgttattcc agtggttttg
     2161 cccttcggag agaactcccc tgcctaatgc tgtagcggca acatgtgtgc catgaccggt
     2221 aattgtattg gcaacattat catctacttc agggaaatag gaataatctt tatactcaaa
     2281 tgacgaagga aaatcagaac ctgcatacga aatatcgatg cccgaatcga gtatacatat
     2341 tttttttcct ttccctttgt accctttagt ccaaaccatt tgggcattga tacttaacga
     2401 agcggaattg ttcatcgccc tgctcgatcg ctcgccccag tcgattttct tcacaaaagg
     2461 aagttcaata actttcttga tgttagctgt tgggacagat gctactataa aaccatatcg
     2521 atggctttca acaggtggag tccaggtgtt gaaataaagt gaaacttcct gatcttctaa
     2581 ctccttaatt tgggaggctg aaggataagc atcaaaatat agaataaccc ggcaagagct
     2641 aataagtgcg tttttcaaat tgcccgaagt tgatttctta ctgaacatca ttgtttcaat
     2701 caattttccg cttaaaatct tgctcaggtt ttgagaagat attttttgtg tgtcattcgt
     2761 ctttaagcct ccgctattct tcggataaag aactgtagaa atgataacaa agacaatcaa
     2821 aagcattacc cgtttcatca tttttaatct atttatgtta aaatatctgt tcgtttatct
     2881 tttaatcaac tttttactga acgtcgtttg tccgcttttc aactcaatca aatacacgcc
     2941 gttcaacatc ccatcaaagg agaaatattc tgttttggaa attatccttg tataaatctc
     3001 cctcccccgg aggtcataaa ttagaactgt tgtatttatg ccatctggat ttacatagaa
     3061 accactggta aatggatttg gataaaccga tttatcgttt aacgcatttt ctgaaacacc
     3121 aacggttaca tcctgaacta cttctactga ttgcgaagcc gctccggttg ctgcaacagt
     3181 cactgtggcc acgcgataag catcatcatt tggatt
//
LOCUS       DAOPUW010000161         3216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_30680_length_3216_cov_2.553939, whole genome shotgun sequence.
ACCESSION   DAOPUW010000161 DAOPUW010000000
VERSION     DAOPUW010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30680_length_3216_cov_2.553939"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="PKJ43_03735"
     CDS             <1..857
                     /locus_tag="PKJ43_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNZ71704.1"
                     /translation="MFTLYMNDPTLINPAYAGTRGNMSINGVFRKQWVGMTWQPTTTS
                     ISANSPFLNNTIGVGFSFMNDQIGPMQQNGFYVDYAYHLLLPNKQNLSLGLKAGFTSL
                     NLNLLDLTVIDWDDVVLMNDYSNAFMPNFGVGAYYYTDKFYAGFSVPLLLKNGLYKDK
                     NRYEIVSREDRVYILTGGGIFTLIEPILKLKPTVLFRAVSGSVPSAELSATLIAYDRV
                     WFGALYRIGDATALHGRFQVTNQLQIGYSYDLTNSDLRPLNRGTHEVLINYVFTRRGE
                     RILSPRYF"
     gene            complement(1085..1954)
                     /locus_tag="PKJ43_03740"
     CDS             complement(1085..1954)
                     /locus_tag="PKJ43_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HNZ71705.1"
                     /translation="MIASSELIINPDGSIFHLHLKPGEIADTILLVGDPGRADMIARF
                     FDTIEVKKENREFVSRTGTYHGKRITVLATGIGTDNIDIVVTELDALVNVDFATREIK
                     PEKKSLNLIRIGTSGSLQPDIPIGAFVVSKKSIGFDGLLNFYAHRDDVSDLAFEECFM
                     NATHWNQSLASPYVVDCSEELHQLIAGSDTVDGINISAPGFYGPQGRVVRLPLADPML
                     NEKIEKFSYNGQRITNYEMESSAIYGLSRLLGHKALTICTIIANRVNLKANETYKDAM
                     EVLIKLVLNRISA"
     gene            2420..3052
                     /gene="def"
                     /locus_tag="PKJ43_03745"
     CDS             2420..3052
                     /gene="def"
                     /locus_tag="PKJ43_03745"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNZ71706.1"
                     /translation="MKREKRLKGWLFLFSVLLIFGCSEEGLNSDEARLIKSGKATEKM
                     RVFTIENSSDSLFLRQPTRKIEKENINSKLITLLRKRMLSTVTDIDNEGVGIAAPQVG
                     VGVKLILVQRFDKAGEPVEPYFNPEIKVYGDSVNAGFEGCLSVPGYRGSVSRSQNIEV
                     SYLDSTGKKQREEINGFVAVIFQHEIDHLSGVMYFDHVFGGFDALLLYKE"
BASE COUNT          896 a          554 c          696 g         1070 t
ORIGIN      
        1 cgatgttcac cttatatatg aacgatccta cgctgattaa ccctgcctat gccggtaccc
       61 ggggaaatat gagtattaac ggggtattca gaaaacaatg ggtgggaatg acgtggcagc
      121 ctacaacaac ttctatttct gcaaattcgc cctttttgaa taatacgata ggagtaggtt
      181 tttcattcat gaacgaccag attgggccta tgcagcaaaa tgggttttat gtcgattatg
      241 cttatcattt gttattgcct aacaagcaga atctttcgtt ggggttgaaa gccggtttta
      301 caagtttgaa ccttaatttg ttagatctta cagtaattga ttgggacgat gtggttttga
      361 tgaatgatta ttcaaatgct tttatgccca actttggcgt gggggcttat tattatacgg
      421 ataaatttta cgctggtttt tcggtaccat tgctcttaaa gaatgggtta tataaagata
      481 agaaccgata cgagattgtg agtcgtgaag accgtgtgta tattctcact ggcggaggta
      541 tttttacttt gattgaacca attctgaagt taaagccaac cgtattattc agggctgttt
      601 ccgggtcggt gccttcggcc gaattgtcag ctaccttgat cgcatacgat cgcgtatggt
      661 ttggtgcact ttatcgtatt ggcgatgcga cggctttgca tggccgcttt caggtaacaa
      721 atcaattgca gattggttac tcgtacgatt taaccaattc agatttgcga ccgcttaacc
      781 ggggcacaca cgaagtgctg ataaattacg ttttcacaag gcgcggagag cgtattttgt
      841 cgccgcgtta cttttaaaaa caatgaagag ctttgccttg tggggctttt aaaaacgttt
      901 tttgttttcc tctatatttt ttttgttttc ctctatttta aaattggaga caattttctc
      961 tcctccgttc tccacggagg agattttttt ttatattgta aagccaacgt ttaaacatcg
     1021 tgatccatag gcgggaaaat aataaaatca gtgccttcag cggaaggaat taaagcagag
     1081 aatattaagc gcttattctg tttagcacca atttaataag cacttccatg gcatctttat
     1141 aggtctcgtt cgctttaagg tttacccggt tggcaataat ggtacaaatg gtaagtgcct
     1201 tgtggccaag aagtcgagat aaaccataga tggcagaact ttccatttcg taattggtaa
     1261 ttcgttgtcc gttgtatgaa aatttttcaa ttttttcgtt caacattggg tcggccagag
     1321 gtaagcgcac cacacgacct tgcgggccat agaagcctgg tgctgaaata ttaatgccat
     1381 caacagtgtc gctgccggca atcaattggt gtaattcttc agaacaatcg actacataag
     1441 ggctagcgag cgattgattc caatgggttg cattcatgaa gcactcttca aaagccaggt
     1501 ccgaaacatc gtcgcggtgg gcataaaaat ttaacaagcc gtcgaaacct attgattttt
     1561 ttgagacgac aaaagcaccg atcggaatat cgggttgcaa gctacccgac gtgccaatgc
     1621 gtatcaggtt taacgatttt ttctctggct taatttcacg ggttgcaaaa tcgacattca
     1681 ccaaagcatc caattcggtt accacgatgt cgatattgtc ggtgccaatg ccggtagcca
     1741 acacagtgat tctttttccg tggtaagtgc ctgtacggct tacgaattca cggttttctt
     1801 ttttaacttc gatggtgtcg aaaaagcggg caatcatatc agctcgtcct gggtcaccta
     1861 ccaaaagaat ggtgtcggca atttcgcctg gttttaggtg caagtggaag atcgatccgt
     1921 cggggtttat tattaattca gatgatgcaa tcatatcgtt tttgtattgg ttagtaccaa
     1981 atctaagaaa aaatctaatt aaaatgcagg aagctggagt taattcattg ggttgtattt
     2041 tgttctaatg atttttctag tttgtataat gttgtaattc agtatcatat gttacttatt
     2101 ttttggtatt tctcaatttt taaattaatt gtattctgat tgagtttttc atgttgatat
     2161 ttataacata tgattattca acaaaatatg agggaatagt gtttctattg taattattac
     2221 ctaagtgatg attcatcaag gattttcata tcgtaatttt tcttaatttt gggttattgg
     2281 ccgttgagtt tgtgttgtag attgctatcg atggtcaatt aatacatgtt tgaaatttgc
     2341 aaaactggct tgtattcgaa ttatagatta tataaataac tacggttgac gtaagagttg
     2401 tctttataaa ttgaaacttg tgaaaagaga aaaaaggtta aaaggttggc tgtttttgtt
     2461 ttcggtgctt ttaattttcg gttgttccga agaaggtttg aatagtgatg aagcccgatt
     2521 gataaaaagc ggcaaagcca ctgaaaagat gcgcgttttt actatcgaga attcttctga
     2581 ttcgcttttt ttacgccagc caacacgcaa aatcgaaaag gaaaacatca attctaaatt
     2641 gattacttta cttcggaaac ggatgttgtc aacagtaacc gatattgata acgagggagt
     2701 aggtattgca gcacctcagg ttggagttgg tgtgaaactt attcttgttc agcgttttga
     2761 taaagcgggc gaacctgttg aaccgtattt caaccctgag atcaaggtgt atggcgacag
     2821 tgtgaatgcc ggtttcgaag gttgccttag tgttcccgga tatcgtggaa gcgtaagccg
     2881 gtctcaaaat attgaagtgt cgtatctcga ctcaacaggt aaaaagcaaa gagaagaaat
     2941 aaatggtttt gttgcagtaa tcttccagca cgaaatcgat catttgagcg gggttatgta
     3001 ttttgatcat gtatttggag gcttcgatgc cctgctacta tataaagaat aagggaatcc
     3061 tgttttttta atattcattc ctactttatt tcagaatagt aattcttttt aacgaagagc
     3121 gaaaactgct tgaagacttg gcagaatcta ttatttttgt caaaaaaatt aatcatgata
     3181 caacgtattc aaaccatatt cttgtcgatt gcccta
//
LOCUS       DAOPUW010000162         3207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_30816_length_3207_cov_3.368972, whole genome shotgun sequence.
ACCESSION   DAOPUW010000162 DAOPUW010000000
VERSION     DAOPUW010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30816_length_3207_cov_3.368972"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PKJ43_03750"
     CDS             <1..283
                     /locus_tag="PKJ43_03750"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HNZ71707.1"
                     /translation="LFLVYTGGIIMSAKAYKKAIAEDAPFAYGKAVGLGVATLFFASI
                     IMALFYYILYAFVDPQLVDQAAALAEEASLQMGLVKKWLNSRWSFSVSC"
     gene            259..423
                     /locus_tag="PKJ43_03755"
     CDS             259..423
                     /locus_tag="PKJ43_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71708.1"
                     /translation="MVIQRKLLSPMFIAVSQMFNVVLYGLFISIITSIFLKKKPENGF
                     NAAMNEIDNE"
     gene            437..1390
                     /locus_tag="PKJ43_03760"
     CDS             437..1390
                     /locus_tag="PKJ43_03760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ71709.1"
                     /translation="MDISVVIPLFNEEESLPELVAWIKKVMDEHHFSYEIIMVDDGSR
                     DSSWAVVQQLSAENPVISGISFQRNYGKAAALHIGFQEAKGDVVITMDADLQDSPDEI
                     PELYRMITKDGYDLVSGWKKVRHDPVLSKNLPSKFYNWTVRRMTKIKLHDMNCGLKAY
                     KKQVIKSIEVYGDMHRFIPVLAKWAGFKKIGEKVVKHQERKYGVTKFGLERFIRGPLD
                     LLSVMFISKFGKRPMHLFGVLGTLMFLVGFFAALWVGVAKIYALKQGIRLALVTDNPY
                     FYIALTSMIIGTQLFLAGFIADLVTRNSPDRNNYQIGDRTK"
     gene            1503..2162
                     /locus_tag="PKJ43_03765"
     CDS             1503..2162
                     /locus_tag="PKJ43_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNZ71710.1"
                     /translation="MTSGGGIQTDYELFLNALLHGDRRSAVTVARKYAGSHNMIKFFY
                     DNIVKSSLYRVGELWEYNSITVAAEHLATSISESIMNDLYENVISDERLPYKVVLGCV
                     ENEQHQVGVKMVADIFEMHGWDTFFLGAGIPISELIDYASGVNPDVIALSLTIYSHLT
                     GLEKMIVAIHQYWPHIPIIVGGQAFLHGGQEVVEKYHQVSLLRSFEEIEQYITKFSDH
                     E"
     gene            2155..2622
                     /locus_tag="PKJ43_03770"
     CDS             2155..2622
                     /locus_tag="PKJ43_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71711.1"
                     /translation="MNREGLIESAGKLISFDSAAVLEYEKKLPIMVQLMNEKMEARND
                     ISDLVGDNVLMMRDNHANHARFILSVMQNFNAEVLVDTILWVFRAYRSRNFHSSYWAA
                     QLNTWLLVFESELSPSAYREIWRIYNWMQVNIPSFVICSDDKLSENKSKHISD"
     gene            2674..>3207
                     /locus_tag="PKJ43_03775"
     CDS             2674..>3207
                     /locus_tag="PKJ43_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71712.1"
                     /translation="MIDNWKEEILQAAIESKTLAIAVFSVDGGLIYCNLAMKKLCKGK
                     ADGNFVNPTLPRLIEITTKETFSGVITFADESNNNYSIEGTASQKNGEIFIFGEVNVA
                     QLVKQNITMTLLNQQINNLQRQLIKEKKMLLKTNADLAELNAEKNRILGIAAHDLRNP
                     VGATSAYADLIKESIDSL"
BASE COUNT          929 a          584 c          744 g          950 t
ORIGIN      
        1 gttgtttctg gtttatacag gcggcattat tatgtcggca aaagcgtata aaaaagctat
       61 cgccgaagat gctccatttg cctacgggaa ggctgtaggg ctgggtgtag ctacgctgtt
      121 ttttgcttca attattatgg ctcttttcta ttatatcctt tatgcctttg ttgacccaca
      181 acttgttgat caggctgcag ccttggctga agaagcctcg cttcagatgg gattagtgaa
      241 gaaatggttg aacagcagat ggtcattcag cgtaagttgt tgagtcctat gtttattgca
      301 gtaagccaaa tgttcaatgt tgttctttat ggcttgttca tttcaatcat cacatcaata
      361 tttttgaaga aaaagcccga gaacggtttc aatgctgcaa tgaacgaaat tgacaacgaa
      421 tagtaaaaca cactccatgg atatatctgt tgtaatacca cttttcaacg aagaggaatc
      481 gctgcccgaa cttgtcgcct ggattaagaa ggtgatggat gaacatcatt ttagctatga
      541 aattatcatg gtcgacgatg gaagccgcga tagctcctgg gccgtagtac agcaactttc
      601 ggcagagaac cctgtaattt ccggcatcag ttttcagcgg aattatggca aagcggcagc
      661 gcttcacatc ggttttcaag aggcaaaggg ggatgtggta attactatgg atgccgattt
      721 gcaagacagc ccggatgaaa ttcctgaact ataccgaatg atcaccaaag atgggtacga
      781 tcttgtttcg ggttggaaaa aagtacgcca cgatcctgtg ctgtcgaaaa acttgccgag
      841 caagttttat aactggacag tgcgtcgtat gaccaaaatt aagctccacg atatgaattg
      901 cgggctcaaa gcatataaaa aacaggtaat taaaagcatt gaagtgtatg gcgacatgca
      961 ccgctttatc cctgttcttg ccaaatgggc cgggtttaag aaaattggag aaaaagtggt
     1021 gaagcatcag gaacggaaat acggagttac taagtttggc ctcgaacgtt ttattcgtgg
     1081 cccgctcgat ttgctttcgg ttatgttcat ttcaaaattc gggaaacgcc ctatgcactt
     1141 gttcggtgtt ctgggtactc ttatgttcct tgtcggtttt tttgccgcat tatgggttgg
     1201 ggtagcaaag atttatgcac tcaaacaagg catccggctg gcattggtca ccgataaccc
     1261 ttatttttac atagccctca cgtcaatgat cattggtact caattgttcc ttgccggttt
     1321 tattgccgac ttggtaacac gcaattcgcc cgatagaaat aattatcaga ttggcgacag
     1381 aacaaagtag aaagtaaaga attttatatt gatagcaagg tgttcagcag agcacctttt
     1441 ttattttatg attgattgct attcataaaa gcagtacctt tcttcagata aacaactttt
     1501 gtatgactag tggtggaggg attcagactg attatgaact ttttctgaat gcattgttgc
     1561 atggagacag gcgcagcgca gtgactgttg cccgtaaata tgcaggttcg cacaatatga
     1621 ttaagttctt ttacgataat attgttaaat cgtcgcttta tcgcgttggc gaactatggg
     1681 agtataatag cataaccgtt gctgcagagc atcttgctac gtctatttcc gaatcaatta
     1741 tgaacgatct ttacgagaac gttatttcag acgaacgctt gccgtataag gtagtactcg
     1801 gatgtgttga aaatgagcaa catcaggttg gggttaagat ggtggccgac atctttgaaa
     1861 tgcatggatg ggacactttt tttcttgggg ccggaatacc catttctgaa ttaattgatt
     1921 atgcatcagg tgttaatcct gatgttatag ccctttcgtt aacaatttat tcccacttaa
     1981 ccgggctaga gaaaatgatt gtggctatcc atcaatactg gccccatatt ccaataattg
     2041 ttggcgggca ggcttttttg catggaggcc aagaggtggt tgaaaaatat caccaagtgt
     2101 cattactgcg gagttttgag gaaattgaac aatatattac aaaattttca gaccatgaat
     2161 agagaaggat tgattgaatc tgccggcaaa ttaataagtt ttgattcggc agcggtgttg
     2221 gagtatgaga aaaaactgcc aataatggtg caactcatga atgaaaaaat ggaggcacgg
     2281 aacgatatta gtgatctggt gggggataat gtgcttatga tgagggataa tcatgcaaat
     2341 catgcccggt tcattttgtc ggtaatgcag aattttaatg ccgaagtcct tgtcgatact
     2401 attttgtggg ttttcagggc ataccggagc cgaaattttc atagcagcta ttgggcggca
     2461 caacttaaca catggcttct ggtttttgag tcagaacttt ctccttcggc ttatcgcgaa
     2521 atttggcgaa tctacaattg gatgcaggta aacataccgt cgtttgtgat ttgttccgac
     2581 gataaattga gcgaaaataa gtcgaaacac atttccgatt gattataagt gtgttgcttg
     2641 agttttttat tacattctag ataaggaaag ccaatgatcg ataattggaa agaggagata
     2701 ttacaagccg caattgaaag caaaacgttg gctattgccg tgttttccgt cgatggcgga
     2761 ttaatatatt gtaacttggc gatgaaaaag ctttgtaaag gcaaggccga tgggaatttt
     2821 gttaacccta cattgccgag gcttattgaa attacaacga aagagacgtt tagcggggta
     2881 attacctttg ccgacgagtc gaacaacaat tattctatcg agggtaccgc ctctcaaaaa
     2941 aatggcgaaa tctttatttt cggagaagtg aatgtagctc aactggtgaa gcagaacatt
     3001 acaatgacct tgctcaatca acaaatcaat aatcttcaac gtcagctcat taaggagaag
     3061 aaaatgttgt tgaaaacaaa tgccgattta gccgaattaa atgctgaaaa aaaccggata
     3121 cttggcattg ccgcacacga cctacgaaat cctgtaggag caaccagtgc gtatgccgat
     3181 ctgattaagg aaagcattga ttcattg
//
LOCUS       DAOPUW010000163         3182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_31149_length_3182_cov_2.804285, whole genome shotgun sequence.
ACCESSION   DAOPUW010000163 DAOPUW010000000
VERSION     DAOPUW010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31149_length_3182_cov_2.804285"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1063)
                     /locus_tag="PKJ43_03780"
     CDS             complement(17..1063)
                     /locus_tag="PKJ43_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNZ71713.1"
                     /translation="MGIASILQVALGSTGIINGLVIAIVLFLLNRGNRKANQLLGLLL
                     VAVCLKLGYALLVNIQHEWKIPSYLIYYCSESAYFAFGPLLLLYSHALLQRPISPFFV
                     AAMFVPAFSPFVGHFISFDIPLWALQSYFFIFLLIIFIKFKLVIFPGEKSSDNHPDRI
                     WLRILFLTFSLIWLTANLLFVDFKFYFLELLAVVIAAIYVVLYSAIRFYWFSEGDKLN
                     VQKYVHSTLTEEETNAIISRLKRVMEVERLYINPEITLPKVAERLQIRPHKLSQVINQ
                     QLNMTFNEYINSCRISEIKGIMKLPEYQNLKIASIAYDYGFNTISSFNTAFKKFTNNT
                     PSQYRNQQLVDNRN"
     gene            1299..2165
                     /locus_tag="PKJ43_03785"
     CDS             1299..2165
                     /locus_tag="PKJ43_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNZ71714.1"
                     /translation="MDKLKKLFHLFMPMVMLFACTGDPLAEVTEESDRLNVSYETVNS
                     EVVGQNYTIYTYLPPNYQTSNDSLPVLYMLDADSEFMTVAKFAEKQIMAKKMVPVIIV
                     GIGYKGNTEDKRNRDYTPTSVSEVTDRIECCGAENFYQFIEKELIPHIDSTCRTQGAN
                     YRAMAGHSLGGLFAFYCFFKHTETFTHYLAASPSVWWDNFVCFEYEEQFSKKASQFDY
                     PTRLMMTLGQAGEGGGGIFPVMVEQLYERLTERGYPQFDAEYVVLNNTVHNENWEDAY
                     QKALPYLFNAKN"
     gene            2188..2850
                     /locus_tag="PKJ43_03790"
     CDS             2188..2850
                     /locus_tag="PKJ43_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71715.1"
                     /translation="MSIIKSFSTKKLVFILLVIVSVNAFSQSDSTRSLSRYDNKLNVS
                     AAINLLPSSFPFSRFLAKDINFSYRFSPKLPVGFTFSVGPGIIIQPKTMKIPGDLTIQ
                     TNHVDFRPGLFIDYPLDAGKYVTFNPRLTAYYPIFFDRSRVESSRYGIDKSKNMFCHY
                     FSPEVELFAEWRWGEMTKLRILKPFTLEGFIKVPVNGIYKIYERGSLGVNLRYFIFTS
                     KK"
BASE COUNT          987 a          683 c          625 g          887 t
ORIGIN      
        1 gtgttgtttt tttgcatcag ttacggttat cgaccaactg ctggttgcgg tactgcgacg
       61 gggtattgtt ggtaaatttt ttgaaagcag tattgaacga tgaaatagtg ttgaacccgt
      121 aatcgtaggc aatgcttgca attttcaggt tctgatattc gggcaatttc ataatgcctt
      181 ttatctcact aattcggcag gagttaatgt actcgttaaa cgtcatgttc aactgctggt
      241 taatcacctg cgaaagctta tgcggcctta tctgaagccg ttcggccacc tttggcagtg
      301 taatttcggg attaatgtat agccgttcaa cctccatcac ccgttttaat ctcgatataa
      361 tcgcgtttgt ttcttcctca gttaatgttg aatgaacata cttctgtacg ttcagcttat
      421 cgccttcgct gaaccaatag aacctaatgg ccgaataaag caccacgtag atagctgcaa
      481 taacaacagc caacagttcg agaaagtaaa atttaaaatc gacaaaaaga aggttggcgg
      541 tcagccaaat cagggaaaag gtcaggaaga gaattctcag ccaaattcga tccggatgat
      601 tgtcagaact tttttcaccc ggaaagataa ccaatttgaa tttgatgaaa ataatgagca
      661 ggaaaatgaa aaaatacgac tgcaaagccc acaagggaat atcgaacgag ataaagtgac
      721 caacaaatgg tgaaaatgcc ggaacaaaca tagcggcaac gaaaaatgga ctgatcggcc
      781 gctgcaacaa tgcatgggaa tagagcaaca gcaagggtcc aaatgcgaaa tatgccgact
      841 ctgagcagta gtaaatcaaa taacttggaa ttttccactc gtgctgaatg ttgacgagca
      901 atgcgtatcc taatttgaga caaaccgcaa ccagcagtaa tcccaacaat tggtttgctt
      961 tccggttccc acggttgagc aggaaaagca caatggcaat taccagccca ttaataatcc
     1021 ctgtagaacc taacgcaacc tgtaaaatac ttgcaatccc catgtttccc ctttttgagt
     1081 ttagacaact ttgttcaagc tttcccttac acgaacctgg gttagatttt tattaagttc
     1141 aaaaattaaa tacaaatcag cgataaatcc tatgttgcac tcatacttca acaaacaagt
     1201 taaaatcctg tcaagctatt ttaaggaaag tcaaaagcaa cccactaaac aactacttgt
     1261 ctttggtatt gaaatttaaa actctgaaca gatatactat ggacaaatta aaaaaactat
     1321 ttcacttatt catgccgatg gtcatgctgt ttgcttgcac aggcgacccg ctggcagagg
     1381 tgaccgaaga atcagatcgt ttgaacgtga gctacgagac tgttaattca gaagttgttg
     1441 gccaaaacta caccatctac acctatctcc cacccaatta tcaaaccagc aacgactctc
     1501 ttccggtgct ttatatgctc gatgccgata gtgagtttat gaccgttgca aaatttgccg
     1561 agaaacaaat tatggcaaag aaaatggtcc cggttattat agtcggaatt ggatataaag
     1621 gcaatacaga ggataaacgc aaccgcgatt atacaccaac atcggtgagc gaagttaccg
     1681 accgtataga gtgttgcggt gccgagaact tttatcagtt tatcgaaaaa gagctgatcc
     1741 ctcatatcga ctcaacgtgc cggacacaag gggccaacta ccgggccatg gcaggtcact
     1801 cgcttggtgg tttgtttgct ttttattgtt tcttcaaaca caccgaaaca ttcacacatt
     1861 acctggctgc aagtccttcg gtttggtggg acaactttgt atgcttcgaa tacgaggagc
     1921 aattctcgaa aaaagcttcg caatttgact acccaacacg gttaatgatg actttggggc
     1981 aagccggcga aggtggtggg ggaattttcc ctgttatggt cgaacaactc tacgaacggc
     2041 ttaccgaacg tggttatccg caattcgatg ccgaatatgt ggtacttaac aataccgtcc
     2101 acaacgagaa ctgggaagat gcctaccaaa aagcattgcc ctatttgttc aacgctaaaa
     2161 actaacccac ttacaaaaca cacagttatg agcatcataa aatcattttc aacaaaaaaa
     2221 ttggtattta tcctccttgt tattgtatct gtaaatgcat tttcgcaatc cgacagcaca
     2281 cggtcgctat cgcgttacga caacaagctg aatgtaagtg ccgcaatcaa cctattgccc
     2341 tcgtcgttcc cgttttcccg ttttcttgca aaagacatca atttcagcta tcgcttttcg
     2401 cctaaactac cggttggatt caccttttcg gtaggtccgg gcattattat ccaacctaaa
     2461 acgatgaaga ttccgggtga tcttacaatc caaaccaacc atgtcgattt caggccagga
     2521 ttgtttatcg actaccctct tgacgcaggg aaatatgtaa cattcaaccc aaggcttacg
     2581 gcctattatc caatcttttt tgatcggtcg agagtagaaa gcagcaggta cggaattgat
     2641 aaaagcaaga atatgttttg ccactatttc tcgcccgaag tcgagctttt tgcagaatgg
     2701 cgctggggcg aaatgacgaa actgcggatt ttaaaaccct ttacgcttga aggctttatt
     2761 aaagtgcccg ttaacggcat ctataaaatt tacgagcggg gaagccttgg ggtaaatctc
     2821 cgatatttca tttttacgtc aaaaaagtag caaccaaatt atcttcctcg aataaaaatc
     2881 ggctctggca tcaatcttat ttttctataa tcctgatttt caattcaaat tttcacatta
     2941 caagtgaaaa agctttttgt agctattttt tttatctgcc tgattaaact ttttgcaggg
     3001 taaataatca aagggaatgt taatcaatct aattattaaa ctatgaaaaa attaatgact
     3061 gtagcttcac ttttgttgct cacaattatc gtaagtgcac agaccgattt ttctggaaca
     3121 tggaagctca ataaagaaaa aagcacattg aatgctgaat tcagcatggc ccctacacaa
     3181 ct
//
LOCUS       DAOPUW010000164         3155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_31538_length_3155_cov_3.003871, whole genome shotgun sequence.
ACCESSION   DAOPUW010000164 DAOPUW010000000
VERSION     DAOPUW010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31538_length_3155_cov_3.003871"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..1674)
                     /locus_tag="PKJ43_03795"
     CDS             complement(268..1674)
                     /locus_tag="PKJ43_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71716.1"
                     /translation="MDFEKIYHNAPEQTKLNFLEAIIMHDDKLKKEFVAYATKNESEI
                     IPFAYEDFTRIASITQAHYQDLFEEVDTENPDWDNYKPPHSGYIPDYEAYQYASKQEF
                     EAIVTGFVKQAIDKIIEQDPPGLMSMFAGLYEAIQNADVDDSVGSFDDVNEYLLGLFS
                     EAMGTVVQKIRLSALSENTICTAFKIFFQYNDEEKTQDPDFLKCFEPLLIALTDKSKK
                     ADSLINIMEDAGIEKELLPELTLSLNKAMGNSAGWLQSAQQFYKNSEPVARELLQYYF
                     ETDKQAFVALARELFDANEFTWANYLKEYMSPQLDNELFVKVYLRLIDKNHDIQYYHK
                     VKEYLTETDIKNLLEKVKWDEVFTVKILEVEQRFEEIKFLIQKDVNKWHFADMIEPIL
                     DIYPEFCFRQIEKLALNTLQAERGRDIYEMIARWLKLAQEIPGFEAEALLLIQKLYTH
                     KPNLPALKDEMRKAGLVK"
     gene            complement(1700..2395)
                     /locus_tag="PKJ43_03800"
     CDS             complement(1700..2395)
                     /locus_tag="PKJ43_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71717.1"
                     /translation="MQPFEILEYGQFYHIYNHGVGGRNLFLEPGNYEYFLGLYDKYIS
                     PIAETYAWCLMPNHFHLLVRIKNNVVYKYSNADGSIDAVRFKEAKWQTVESNLTASEG
                     PVSVGENRIKIPQPHLHFSHLFNAYSKYLNSRHEARGALFERPFRRKLIDNLNYRREV
                     VVYIHCNPIHHGFCTHPVEYPWSSFLSCTSDKATKLKRDEVIGWFGGKNEYEEWHKKK
                     TDMHKMDWELGID"
     gene            complement(2462..>3155)
                     /gene="cas6"
                     /locus_tag="PKJ43_03805"
     CDS             complement(2462..>3155)
                     /gene="cas6"
                     /locus_tag="PKJ43_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HNZ71718.1"
                     /translation="IYKVLGKADAGFAGFLHSEGYRDGAKSFKLFCFSPLNFGKPTLW
                     KEKTLFEIHQENVTLSVSFYLPDAAERFIVGLFNNQQAYFGDRFNGIDLTVVQIERLP
                     EAVVAPTVHYRALSPVVISARSEEARYARYLSPTDEGYALMVKNNLTQKWATIPGALM
                     LPEPFDFEMVVTSQPKSKLVTMKPGTPQQSKVRGYVYEFDLTAPEALHQLIGSAGIGE
                     KNSTGFGWCEVK"
BASE COUNT          793 a          722 c          652 g          988 t
ORIGIN      
        1 cagatgttgt tttaatccat ttctcttaat attgaggaat ggattttttt atttcatgct
       61 actcttcttt cattttcctc gtttatattt ttcgtctacc ttctttcgcc aactctatca
      121 ggagcttcgc acgctgacag gttttggaag ttggacaaac ccgacagcgt cgttagttga
      181 atacttatta ttcgttgttg cctcctaacg ctgactggtt tttcatcccg acgctgacag
      241 gtcctgcgga cgctgtcagc gttggggcta cttcaccagc ccggcctttc gcatttcatc
      301 ttttaacgcg ggaaggttgg gcttgtgtgt gtatagtttc tgtatcagta aaagtgcttc
      361 ggcttcaaaa cccggtatct cttgagccaa tttcagccat cgggcaatca tctcatagat
      421 gtcacgtccc cgctcggcct gtaaggtatt caatgccagt ttttcgatct ggcggaaaca
      481 gaattcgggg tatatatcca atattggctc aatcatatcg gcaaagtgcc atttattgac
      541 atccttttga atcaaaaatt ttatttcctc aaagcgttgt tctacttcaa gtattttcac
      601 cgtaaacact tcgtcccatt tcactttttc aagcagattc tttatatcgg tttcggttag
      661 gtattccttt actttatggt aatattgaat gtcgtggttt ttgtcaatga gcctgagata
      721 aacctttacg aacagctcgt tatcaagttg cggactcata tattctttca aataattggc
      781 ccacgtaaat tcattggcat cgaacaattc gcgggcaagg gcaacaaaag cttgtttatc
      841 ggtttcaaaa tagtattgaa gcagttccct ggctacaggc tcgctgtttt tataaaattg
      901 ctgggctgat tgcagccatc cggctgaatt gcccatcgct ttgttgaggg aaagtgttag
      961 ctcgggcagt aattcctttt caattcctgc atcctccata atatttatta aactgtcggc
     1021 tttttttgat ttatcggtca gcgcaatcaa taacggttca aaacatttca agaaatcggg
     1081 atcctgggtt ttttcttcat cattatattg gaaaaagatt ttaaaagcag tgcagattgt
     1141 attttcagac agtgcactta acctgatttt ctgcaccact gtacccatag cttcggagaa
     1201 caacccgagc aaatattcgt tcacatcgtc gaacgagccg acactgtcgt ctacatcagc
     1261 attttgaatt gcctcgtaaa gccctgcaaa cattgacatc agtccaggcg ggtcttgttc
     1321 aatgatttta tcgatggctt gctttacaaa accggttaca atagcttcaa actcctgttt
     1381 gctggcatat tggtaagcct cgtaatcggg gatgtaccct gaatgaggcg gtttataatt
     1441 gtcccagtct gggttttcgg tatccacttc ttcaaacaaa tcctgatagt gagcctgtgt
     1501 aatactggca atccgggtaa aatcttcgta agcaaacggg attatttcac tttcgttttt
     1561 tgttgcatag gctacaaatt cttttttaag cttatcgtcg tgcatgatta tagcctccaa
     1621 aaaattgagc tttgtctgct caggtgcatt gtggtatatt ttctcaaaat ccataaataa
     1681 aatcttaatt ccgttgtttt caatcaattc ctaactccca atccatttta tgcatatcgg
     1741 ttttcttttt gtgccactct tcgtattcat ttttaccacc aaaccaaccg attacttcgt
     1801 cgcgtttaag tttggtcgct ttatcagatg tacaggacaa aaaactgctc cagggatatt
     1861 caacagggtg tgtacaaaaa ccatgatgga tcgggttaca atgtatgtac acaactactt
     1921 ccctgcgata attcaggtta tcaatcagtt tccgtctaaa cggccgctcg aacaaagcgc
     1981 cgcgggcttc atggcgtgaa ttgaggtatt tgctatatgc gttgaacaga tgcgaaaaat
     2041 ggagatgtgg ctggggaatt ttaattctat tttctccaac gctgacaggt ccttcggacg
     2101 ctgtcaggtt ggattcaacc gtttgccact tcgcttcttt aaacctgacg gcgtcgatag
     2161 acccgtcagc gttggaatat ttataaacaa cattattttt aatcctaacc agcaaatgaa
     2221 agtggttcgg catcaaacac cacgcatacg tttcggcaat gggcgatatg tatttgtcgt
     2281 acaaccccag aaaatattcg tagtttccag gttcgaggaa caaattgcgc ccgcccacac
     2341 cgtggttata gatgtggtaa aactgtccgt attcaagtat ttcaaaaggt tgcatttttt
     2401 tcgttttttt atccttaaac ctgacagatt tttgaaacct gccaggttta aatgttgatt
     2461 attatttcac ctcgcaccat ccaaaccctg tagaattttt ctcgcctatc cctgccgaac
     2521 caatcagttg gtgcagcgct tcgggggctg taaggtcgaa ttcgtacaca taaccgcgca
     2581 ccttgctctg ttgcggggtg ccaggtttca tggtcaccag ctttgatttg ggctgactgg
     2641 taacaaccat ttcgaaatcg aacggttcgg gaagcatcaa cgctccggga atggttgccc
     2701 atttttgcgt caggttattc ttcaccatca gggcataccc ttcgtcggtg ggagaaagat
     2761 aacgggcata acgcgcctct tcgcttcggg cgcttatcac caccggcgat agtgccctgt
     2821 aatgcacggt tggggcaaca acagcttcgg gcagtcgctc aatctgaaca accgtcaggt
     2881 cgatgccatt gaagcggtcg ccaaaataag cctgctggtt gttgaagagc cccacaataa
     2941 accgctcggc cgcatcgggc aaatagaacg aaaccgaaag agttacattc tcctgatgga
     3001 tttcaaaaag ggttttttct ttccacagtg tcggcttgcc aaagtttaac ggggaaaaac
     3061 aaaacagctt gaagctttta gccccatcgc ggtaaccttc ggaatgcaaa aaaccggcaa
     3121 acccggcatc ggcctttccc agcaccttat agatc
//
LOCUS       DAOPUW010000165         3141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_31747_length_3141_cov_3.269605, whole genome shotgun sequence.
ACCESSION   DAOPUW010000165 DAOPUW010000000
VERSION     DAOPUW010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31747_length_3141_cov_3.269605"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PKJ43_03810"
     CDS             complement(<1..558)
                     /locus_tag="PKJ43_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNZ71719.1"
                     /translation="MIAAISYSFLQEYWWAIISLLGSLLVFLMFVQGGQSLLFIIGKT
                     ELQRTMLVNTLGRKWEFTFTTLVTFGGAFFASFPLFYATSFGGAYWVWIAVLFSFILQ
                     AVSYEFRSKPSNFLGKKTYEIFLFINGLMGPILIGTAVGTFFNGACFSLNDYNQVIWQ
                     GASRGLEAVAVFHNVALGLAVFFLVR"
     gene            complement(555..2129)
                     /locus_tag="PKJ43_03815"
     CDS             complement(555..2129)
                     /locus_tag="PKJ43_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNZ71720.1"
                     /translation="MFESIDLSLVDWSRAQFALTAMYHWLFVPLTLGTTFMIAIMETI
                     YVRTGNPEWKKMTKFWMTLFGINFAIGVSTGIILEFEFGTNWSNYSWFVGDIFGAPLA
                     IEGIMAFFMESTFIAVMFFGWNKVSPKFHLLSSWLVAIGANLSALWILVANGWMQSPV
                     GTTFNPETARNEMTSFWDVLFSPVAVNKFLHTITSGFVLSAVFVIGVSAWFLLKKRHV
                     VLARKSILVASIFGIVSTLAVIGTGDGSAKQVYKVQPMKFAAMEGLYEGGNGVAIKVF
                     GLLSNSEADPEYSKLKDFVFKFEIPKFLSILAANDPNAYVPGVKDIIEGNEQQGILPV
                     AEKIERGKLALSTLSEFKEAKKNGDLQKASELRTLLISDSFQTNNFKYLGYGHIKDVN
                     YLIPNIALTFYAFHIMVMLGVFFLVLFVLAFWWLIKGSLEKQRWFLWIALFSIPLAYL
                     ASELGWVVAEVGRQPWIIQDLMTTSTAVTNISTSSVKITFVLFAVIFTTLLIAEIGIM
                     VKQIKIGPKEVEGGSK"
     gene            complement(2136..2348)
                     /locus_tag="PKJ43_03820"
     CDS             complement(2136..2348)
                     /locus_tag="PKJ43_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HNZ71721.1"
                     /translation="MNIAKRVFRFYYDGFKNMTWGKSVWIVILVKLFIFFVIMKLIFF
                     PNFLKKNFDTDAERSSHVLENITNSK"
     gene            complement(2563..>3141)
                     /locus_tag="PKJ43_03825"
     CDS             complement(2563..>3141)
                     /locus_tag="PKJ43_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71722.1"
                     /translation="QTRSIQLVSKESALTDNDLLSVCIAKGGTSVVADGYLICGDMTI
                     EQEWFCFGFGTLLQFIDDIQDVNEDCSGQLETVFTRGALSGKLEELTNRTLSFTHSVL
                     EKINYCYRSSVTPSVRGLMEKSVVSMLSEAVALNVQYFSPVYSSAFELHSPFRYSFIR
                     KRRNNSDPSRISLMKTIEGYIFGKEKAVVGIS"
BASE COUNT         1037 a          727 c          566 g          811 t
ORIGIN      
        1 ccgaactaaa aagaaaacag ctaaccccag tgccacgttg tgaaaaactg ccacagcctc
       61 gagcccacgc gatgcgcctt gccatatcac ctgattataa tcgttgagcg aaaaacatgc
      121 accattgaaa aaagtaccta cggcagtacc tatcagtatt ggccccatca atccattaat
      181 aaagagaaat atttcatagg ttttctttcc aaggaaatta gaaggtttag agcgaaactc
      241 gtacgacaca gcctgcaaaa taaacgagaa caaaacggca atccataccc agtaggcgcc
      301 tccaaaactc gtagcataaa acagtgggaa cgatgcaaaa aaagctccac caaaagttac
      361 cagggtggta aacgtgaatt cccacttccg ccccagcgtg ttcaccagca tagttcgctg
      421 taattcggtt ttgccaataa tgaacagcaa cgattgcccg ccttgaacaa acataagaaa
      481 tacaagcaac gagcccagca atgaaattat tgcccaccaa tattcctgta agaatgaata
      541 acttatcgca gcaatcattt tgagcctcct tccacttctt tgggtcctat tttaatttgc
      601 ttaaccataa ttcctatctc agcaatgagc agagtagtaa aaataacggc aaaaagcacg
      661 aaagtaattt tcaccgatga agtgctaata tttgtcacgg cagttgaggt tgtcattaaa
      721 tcctgaataa tccatggctg gcggccaact tcggcaacca cccaccccag ctctgaagca
      781 agatatgcca gcggaatgga aaacaaggca atccacaaaa accaacgttg cttttcaagc
      841 gatcctttta tcagccacca gaaagccagc acaaacaaaa caaggaagaa aacgccaagc
      901 atcaccataa tatgaaatgc ataaaacgta agggcgatgt tgggaatcag ataatttaca
      961 tcttttatgt gtccgtatcc cagatactta aaattgttcg tttggaagct gtcgctaatc
     1021 agcagcgtgc gtaattcact ggctttttgc agatcaccgt tttttttggc ctctttaaac
     1081 tcgcttagag ttgacaaggc aagtttcccg cgttcaattt tttctgcaac aggaagtatc
     1141 ccctgctgtt cgttcccctc aataatatct tttaccccgg gtacataggc attggggtcg
     1201 tttgctgcca aaatggaaag aaattttgga atctcaaact taaaaacaaa gtctttcaac
     1261 ttgctgtatt ccgggtcggc ttcgctgttc gacaataatc caaaaacttt aatggctaca
     1321 ccattgccac cttcataaag cccttccatt gcggcaaatt tcatgggctg aactttgtac
     1381 acctgcttag ccgaaccatc gccggtgcca ataactgcga gcgtcgatac tattccgaaa
     1441 atggaggcca caagaatact ttttcgggct aacacaacat gccgtttttt tagcagaaac
     1501 caggccgaaa caccaatcac aaaaactgct gaaagaacaa atcccgatgt aatggtatgc
     1561 aaaaatttat ttactgctac aggagaaaat aaaacatccc aaaaactagt catctcgttt
     1621 cttgccgttt cgggattaaa tgttgttcca accggactct gcatccaccc gttggccacc
     1681 aaaatccaca atgccgagag attagcccca atggcaacca gccacgacga gaggagatga
     1741 aatttgggac tcactttatt ccacccgaaa aacattacgg caataaaagt cgattccatg
     1801 aaaaaggcca taatgccctc aatggcgagt ggcgcaccaa aaatatcgcc cacaaaccag
     1861 gaataatttg accagttggt gccaaattca aattcgagta ttataccggt tgatacacca
     1921 atagcgaaat taatcccaaa aagtgtcatc cagaatttgg tcattttttt ccattcagga
     1981 ttgccggttc gaacataaat tgtttccatt atggcaatca tgaatgttgt gcccaatgtt
     2041 aacggaacaa ataaccaatg gtacattgcc gttaacgcga attgcgcacg cgaccagtca
     2101 actaacgaaa gatcgattga ttcaaacata ggtgtttatt ttgagttagt tatgttttca
     2161 agcacatgcg agctccgctc agcatcggta tcgaagtttt tcttcaggaa atttgggaaa
     2221 aaaatcagct tcattattac aaagaagata aacagcttaa ccagaataac aatccacacc
     2281 gactttcccc aagtcatgtt tttaaatccg tcatagtaaa acctgaatac ccgtttagca
     2341 atgttcattc ttgctgaatt tagtgtgttt aaattaatct ttatacacga gaaaacaaaa
     2401 cattttttac aaaccaaatg tgactaacgc aagaaagccc ggcaaaagtg cgggcttcaa
     2461 actgaaaacg attgcttaaa agcttaattt aaaaatagta aagtaatgta tgatgaaata
     2521 atgatttaat tttattagga aaaactgtca gcataaaaga ttttacgata ttcctaccac
     2581 tgctttttcc ttaccgaaaa tataaccttc aatagttttc attaacgata tacggctcgg
     2641 atccgaatta ttacgtctct tacgaatgaa tgaatatctg aatggggaat gtagttcaaa
     2701 tgcagatgaa tataccggcg agaaatactg aacattcaag gcaaccgcct cactaagcat
     2761 cgagactaca ctcttttcca ttaagcccct caccgaaggc gtaacactgg agcgataaca
     2821 ataattgatt ttttccaata cagaatgcgt aaacgacagg gtccggttgg tcaattcttc
     2881 gagtttacca gaaagggcac ctcgggtaaa aactgtttca agctgtcccg aacaatcctc
     2941 attaacgtcc tgtatatcat caatgaactg caataacgtt ccaaaaccga aacaaaacca
     3001 ttcttgttca atggtcatat ctccacaaat aaggtatccg tcggctacta cagaagtgcc
     3061 cccctttgcg atacaaactg acagcaaatc gttgtcagtc aacgcagatt ccttactcac
     3121 cagctgaata ctccgggttt g
//
LOCUS       DAOPUW010000166         3136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_31831_length_3136_cov_2.751704, whole genome shotgun sequence.
ACCESSION   DAOPUW010000166 DAOPUW010000000
VERSION     DAOPUW010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31831_length_3136_cov_2.751704"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /gene="corA"
                     /locus_tag="PKJ43_03830"
     CDS             <1..1060
                     /gene="corA"
                     /locus_tag="PKJ43_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNZ71723.1"
                     /translation="RFLKNRQNTLGKSPGTLTFIGRKKMEQPRFRLISYSNNSYGDKE
                     YHSYQQVIEAIDPSHVCWINIDGIHDPDVVAGICNYAGVSPLTQEDIVNTDRRPKVTE
                     EKDSLVVILKVLSFNKDRKNIASDQITFILAQNLLITFQEKVGDYFNPIRERLEQNFG
                     KLRSSSADYLLYRLIDAMADNYLVTIGMLGEMIEKNEDLLLRKNNPQFIRDIYSYKTE
                     ISFLRKIIRPAKEVSKILKNSDTNLIANSTQPYLDHLDDLLTYALETVETYYAMTSDQ
                     LTIYNTNINNRSNEVMKVLTIYASIFIPLTFIVGVYGTNFDFVPEYHFRYGYYAMWAV
                     MLILSFAMLVYFRRKKWL"
     gene            1105..>3136
                     /locus_tag="PKJ43_03835"
     CDS             1105..>3136
                     /locus_tag="PKJ43_03835"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNZ71724.1"
                     /translation="MRRIIILVISFLSVIQIFAQTNQIKFRSLDINKGLSSNQINTIF
                     KDSQGFLWIGTVNGLNRFDGYSTRVFRKTADSAGSSLNNNIFRIYEDCQGRLLILSGE
                     ELWVFDTATERFLSDDELFNLNIPIPRSGIVEIIRLNSNSLWFITRDSGLFGYNHQKK
                     EVVHIQPSQLGGVNASNNRISGATASPDGMLWLVKSDLSLAKVDVVGRRIVQTILSLS
                     AYLRPSDVDHGIFADSHSNLWIFSRNENEGLLRYNLSLGQANVYETSESAGSINSNVI
                     TAVIEDDFGNILVGTDHGGLSRIHSISGKIETYKNDPADKNSLAQNSITCLYRDNVGI
                     IWIGTYKKGVSYYHPDLFKISTYILHPFKENWLGYDDVNTFAEDSQGNLWIGTNGGGL
                     FYFDRQKNIFKSYKHNPSDNNSLSSNVVVDVLVDHLGDVWVGTYMGGLNRFDGRKFTR
                     FMHNPSDNTSIAHNNIWSLFEDSERNLWIGTLGGGLDRFDRNTGQFVHHQTSMENSVH
                     SDFIMAVDEGIDGNLWLATTQGFEQFDRDKNIFTQFAQEPDGKGLISNTALDVFCDHR
                     GWVWVATREGLNMYDPKNKKHRHFTADDGMPINDVLTILEDDKGNMWLGTPLGLCNLI
                     VSSKPDGSYDFRCKNYDEKDGLHSKAFNEHGALKTRRGELVFGGPEGFSVFKP"
BASE COUNT          899 a          630 c          696 g          911 t
ORIGIN      
        1 acgatttctt aaaaaccggc aaaataccct tggtaaatct cccggaacat taacttttat
       61 tggaaggaag aaaatggagc agcctcgttt caggttgatt tcctattcca acaattctta
      121 tggagacaaa gaatatcatt catatcaaca agttattgag gctattgacc catctcatgt
      181 ttgttggata aatatcgatg gtattcatga tcccgatgtg gttgccggca tttgcaatta
      241 tgccggtgtt tcgcccctta cccaggaaga tattgttaat accgaccgtc gtcctaaggt
      301 tacggaggaa aaggatagtt tggttgtgat tcttaaggtg ttgtcattca ataaagaccg
      361 taaaaatatt gcttcagacc aaattacctt tattctggcg caaaacttgt tgatcacttt
      421 tcaggaaaaa gtgggggatt attttaatcc gattcgggaa cggttggagc aaaattttgg
      481 taagctaaga tcaagtagtg ccgattatct tttgtaccgt cttattgatg ctatggccga
      541 caattacttg gttaccatag gtatgcttgg tgaaatgatt gaaaaaaacg aagatctctt
      601 gttgagaaaa aacaatcctc agttcattcg cgatatttat tcttacaaaa cagaaataag
      661 ttttttgcgg aagattatcc gccctgccaa agaagtctcg aaaattctca agaattcaga
      721 cactaacctt attgccaatt caacacaacc ttacctcgac cacctcgacg atttgctgac
      781 gtatgccctg gaaacggttg aaacatatta tgccatgacc agtgaccagt tgaccattta
      841 taatacaaac attaataacc gctcgaacga agtgatgaaa gtgctcacaa tttacgcgtc
      901 tattttcatc ccgttaacct ttatagtcgg ggtatacgga acaaactttg atttcgtgcc
      961 cgaatatcat ttccgctatg gatattatgc gatgtgggct gttatgctta ttttaagctt
     1021 tgccatgttg gtttatttca ggcgtaaaaa gtggctctaa tctgtgaaaa aataaaaaga
     1081 atcattgaac acaaaattat tgccgtgagg agaatcatca tacttgtaat tagtttttta
     1141 tctgttattc agatttttgc acagacgaat caaattaagt ttcgcagtct cgacatcaac
     1201 aaaggcttgt cgagcaacca aattaatacg atatttaagg acagtcaggg ttttttatgg
     1261 attggcacgg tgaacgggtt aaaccgtttc gacgggtatt ctacccgggt attccgaaaa
     1321 acggccgata gcgcaggctc ttcactcaat aataatattt tccgcattta tgaagattgt
     1381 caggggcgcc tgcttatcct ttcgggcgaa gagctatggg tgttcgatac tgccaccgaa
     1441 cgcttccttt ccgacgatga acttttcaat cttaatatcc cgatccctcg ttcgggcatt
     1501 gttgaaatca tacggttgaa cagtaattcc ttatggttta tcactcgcga ttcggggctc
     1561 tttgggtaca atcaccaaaa gaaagaggtg gtacacatcc agccttcgca actgggtgga
     1621 gtgaatgcgt ctaataaccg gatttcgggc gctacagctt ctcccgatgg aatgctttgg
     1681 ctggtgaaaa gtgatttgtc gcttgcgaaa gtcgatgtcg ttggccggag aattgtgcaa
     1741 accattcttt ccctttccgc gtatttacgt ccttcagatg tagatcatgg tatttttgcc
     1801 gactcgcaca gcaatctttg gattttttcc cggaacgaaa acgaaggctt gctccgatat
     1861 aacctttcgc tgggtcaagc aaatgtttat gaaacctctg aaagtgcagg aagcattaac
     1921 agtaatgtaa ttacggcagt aattgaagac gatttcggca atatccttgt tggtaccgac
     1981 catggaggat taagtcgtat ccattcaatc tcggggaaaa ttgaaaccta taaaaacgat
     2041 ccggccgata agaacagcct cgcacaaaac agtattacct gtctttatcg tgataatgta
     2101 ggaataatat ggatcggaac atataaaaaa ggggttagct attatcatcc tgatctgttt
     2161 aaaatttcta cttacattct tcaccccttt aaagaaaatt ggctcgggta cgacgatgtg
     2221 aacacatttg ccgaggattc acagggaaac ctgtggattg gaaccaatgg cggagggttg
     2281 ttttatttcg acaggcagaa aaacatattt aaaagttaca agcataatcc cagcgataat
     2341 aactcgttga gcagtaatgt ggttgtcgat gtgttggttg accatctggg cgatgtttgg
     2401 gtgggtactt atatgggcgg gcttaaccgt ttcgatggaa gaaaatttac ccgatttatg
     2461 cataaccctt ccgacaatac ctcaattgcc cacaacaata tatggtcgct tttcgaagat
     2521 tcggagcgca acctatggat tgggacctta ggcggagggc tcgatcgctt tgatcggaat
     2581 acgggtcaat ttgtccacca tcagacgagt atggagaaca gcgttcattc cgacttcata
     2641 atggctgtcg acgaaggtat tgatggcaat ttgtggctgg ccactaccca gggttttgaa
     2701 cagtttgacc gcgacaaaaa catatttacc caatttgcac aagagcccga cgggaaaggc
     2761 ttgataagca ataccgcgct tgatgtattt tgcgatcacc ggggttgggt ttgggtggct
     2821 acccgcgaag gattaaacat gtacgacccg aaaaataaaa aacaccggca ttttaccgct
     2881 gacgatggca tgccaatcaa cgatgtatta actattcttg aagacgacaa agggaacatg
     2941 tggcttggaa caccccttgg tttgtgtaat ctcattgtca gttcaaaacc cgatggcagc
     3001 tatgatttcc ggtgcaagaa ttacgatgag aaagacgggc ttcatagtaa agccttcaat
     3061 gaacatggcg cacttaaaac ccgtcgtgga gagttggtct ttggtggccc cgaagggttt
     3121 agtgttttta aacccg
//
LOCUS       DAOPUW010000167         3132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_31883_length_3132_cov_3.479038, whole genome shotgun sequence.
ACCESSION   DAOPUW010000167 DAOPUW010000000
VERSION     DAOPUW010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31883_length_3132_cov_3.479038"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PKJ43_03840"
     CDS             complement(<1..604)
                     /locus_tag="PKJ43_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ71725.1"
                     /translation="MEQKLKYLIVEDEAKSRETLLQKIEMCNIPNIVCTGMAANSTEA
                     MMLAKLTPPDFILLDINLPGKNGFELIEELKSINISPQVIFTSAHTESSILLDALKKS
                     PVTYLTKPIDLDDLEEAIRKVILLIKNEKPNKTNDQKIKLNAYSGPIYLYADQIMMVK
                     SELHQSKLYFNDGECILLNQNIGSFEKDHPLCELPFFRADR"
     gene            complement(617..>3132)
                     /locus_tag="PKJ43_03845"
     CDS             complement(617..>3132)
                     /locus_tag="PKJ43_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNZ71726.1"
                     /translation="YQIRYNLKSKKCDVLYSGDNKVLYSKIEQDDCLFFTSDTLCIFN
                     HTGLHKQALPGKFTCYVENQSDSTTYGISETDNKIYKLDPLFSSATLIMKNTKINLNF
                     RLIADLANNNSLYFVNKQNEICKLGCGPLGRNTIISCMLIDRENNLWVGTENGIYNYF
                     KAGFEEHKFNIGKSVDNVWSIIQTADSTMWFGGYLTGFWSLDKKDRLTVYNANTYLDN
                     ELQEYNNTFYMGGITDKEGRAYLPVKHGILRIENNKIKHFRIDKYNVPMSITDDPGNN
                     RLLMGTSAGFCVMEKGTMKIIDLVRSPRSIVSTCIDKKGNILAGSFTAQFIYQQDTLI
                     PYHPERNRGAISMVKDFRGNVWKGTSTGLYLDNGDDESQQFDEKIKGMIASVHISHPY
                     LLAATVNRLYMVNLDSFYNNPNALLYEFDPGNGYIAMDGGQNGFYTDNEGYVWYTVTD
                     KVLRFSPEDLAQSYFRHSPKPHFVSFNFSSDQVSWNRVEPHDTLNLKRKWNENSVRFN
                     INAVSATYADNMVYQYRIKQLSPEWSEPTQITQQTFSNLRPGKYQIEIRCSCHDGVWS
                     DVVTSPLITIKRAWWQFWWVGLLMALMIIGLIIRLTVLIVKQRQKYLIRKLNEQKRLN
                     ELRLQTVRSKHIPHFSGNALSNIEHFIFSADLRQANKYLTKYSRLMNIMLRDADKASR
                     SIEQEIEYVTLYLELEKMRFEDKLEYEIQLQPDIDTRKEIPNMLLQTWVENALKHGLR
                     HKDGVGKITIIIKNSTGYTEISVEDNGIGREKAKELGTTGTGQGLKILAEQIEIYNHF
                     NKSKIEIRTLDLSDENRKATGTRFIIFIPDNYNFTSLEN"
BASE COUNT          838 a          630 c          627 g         1037 t
ORIGIN      
        1 tacggtcggc gcggaagaaa ggtaattcgc acaatggatg atctttctca aaagaaccaa
       61 tgttctgatt tagcagtatg cactcgccat cgttaaaata taattttgac tggtgcaatt
      121 ccgacttcac catcataatt tggtcagcgt aaagatatat tgggccactg taagcattta
      181 atttaatttt ttgatcattt gttttattgg gcttttcatt ctttattaat agaataactt
      241 ttcttatggc ttcctccaag tcatccaaat cgataggctt ggttaaatag gtgaccgggg
      301 actttttcaa tgcgtcgagt aaaatcgaac tttcggtatg agcgctggtg aaaattacct
      361 gaggtgaaat atttatcgat ttcaattctt cgatcaattc aaagccgttt ttaccgggaa
      421 gattgatgtc gagcaggata aaatcgggag gagtaagttt ggcaagcatc atggcttctg
      481 ttgaattcgc tgccattccg gtgcaaacta tgttggggat attgcacatt tcaatttttt
      541 ggagcagtgt ctccctcgat tttgcctcat cttcaacgat caggtacttt aatttctgtt
      601 ccatactcac attgctttag ttttcgagac ttgtgaaatt gtaattatca ggtataaaaa
      661 taataaaacg ggttcctgtt gccttcctgt tttcatcgct taggtcaagt gtccttattt
      721 caatcttcga tttgttgaaa tgattataaa tttcaatctg ttcggcaagg attttaagtc
      781 cctgtccggt tcctgttgtt cctaattctt ttgctttttc tcgaccaatg ccattatctt
      841 caaccgaaat ttcggtataa ccggtgctat tttttatgat gatagttatt ttaccaaccc
      901 catctttatg ccttaaccca tgttttaggg cattttctac ccaggtttgc agcagcatgt
      961 tgggaatctc tttgcgggta tcaatatcag gttgaagttg aatttcatat tccaatttat
     1021 cctcaaaacg catcttttcc aattcgaggt aaagggttac atactcaatt tcttgctcaa
     1081 tcgatcggct ggctttatcg gcatcgcgca acatgatgtt catcaaccgt gagtattttg
     1141 tgaggtattt gttggcctgg cgtaaatcgg cactgaaaat aaagtgttcg atattcgata
     1201 gggcattacc cgaaaaatga gggatgtgct tcgatcgtac tgtttgaagg cgtaattcat
     1261 taagcctttt ttgttcgtta agtttcctga tcaggtattt ttgccgctgc ttaacaatta
     1321 atacagttaa ccttataata agtccgataa tcatgagtgc catcaaaagg ccgacccacc
     1381 aaaattgcca ccatgcccgc ttaatggtga taagcggtga ggtaaccaca tcggaccaaa
     1441 cgccatcgtg acaagagcaa cggatctcga tttggtattt tccggggcga aggttcgaga
     1501 aggtttgctg ggtaatttgg gttggctccg accattcggg tgaaagttgc ttaatccggt
     1561 attggtaaac catattgtcg gcataagttg ccgaaactgc attgatgttg aaacggaccg
     1621 agttttcatt ccatttgcgc ttcaggttaa gggtgtcgtg cggttcaacc ctattccagg
     1681 aaacttgatc ggacgaaaaa ttaaaagaaa caaaatgtgg ttttggagaa tgtctgaaat
     1741 aactttgggc caggtcttcg ggcgaaaaac gcaatacctt atcggttaca gtataccaaa
     1801 catatccctc attatcggtg taaaaaccgt tttgtccgcc atccatagct atatatccat
     1861 ttcccggatc aaattcatat aacaaagcat tcgggttatt gtaaaacgaa tcgaggttga
     1921 ccatgtatag ccggttaacc gtggctgcca gcaggtaagg gtgactgatg tgaaccgaag
     1981 caatcattcc cttaattttt tcatcgaatt gttggctttc atcatctcca ttgtcgaggt
     2041 ataaaccagt tgaggttcct ttccatacgt tgcccctgaa atctttcacc atcgaaatgg
     2101 cccctctgtt gcgctcggga tgatatggta tcaatgtatc ttgctgatag ataaattgcg
     2161 ccgtgaaaga tccggccaaa atgttgcctt ttttatcgat acaggtactg acaatgctac
     2221 ggggagaacg aactaaatca atgatcttca ttgttccctt ttccattaca caaaagccag
     2281 ccgatgttcc catcagcagc ctgttgttcc cggggtcgtc ggttatcgac atggggacgt
     2341 tatacttatc aatccgaaag tgcttgatct tgttattttc aattcttaaa ataccatgtt
     2401 ttaccggtaa atacgccctg ccttccttat cggtaatacc tcccatataa aaggtattat
     2461 tgtattcctg taattcattg tcgagatagg tatttgcgtt atacacggta agcctgtctt
     2521 ttttatcgag cgaccaaaaa ccggttaaat agcctccaaa ccacatcgtg ctgtcggcgg
     2581 tttggataat tgaccaaaca ttatcgaccg atttgcctat gttgaatttg tgttcctcaa
     2641 aacccgcttt gaaatagttg taaatgccat tttcagtccc tacccaaagg ttattttccc
     2701 tatcgatcaa catgcacgat attatcgtat tgcgtcctag tggtccgcat cccagtttac
     2761 aaatctcgtt ttgtttgttc acgaaatata atgaattgtt atttgctaag tcagcaatca
     2821 atctgaaatt cagattgatc ttagtatttt tcattattag cgtggctgat gaaaacaagg
     2881 ggtcgagttt gtaaatctta ttatcggttt cacttatgcc atatgtcgtt gaatcacttt
     2941 ggttttcaac ataacaggta aatttgccgg gcaacgcttg cttatgcaaa ccagtatggt
     3001 taaaaatgca caaggtgtcg ctggtaaaaa aaagacagtc atcttgttca attttactat
     3061 ataaaacctt gttatcaccc gaatacaaca catcgcactt tttgctttta agattgtacc
     3121 gtatttggta ga
//
LOCUS       DAOPUW010000168         3123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_32014_length_3123_cov_2.790743, whole genome shotgun sequence.
ACCESSION   DAOPUW010000168 DAOPUW010000000
VERSION     DAOPUW010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32014_length_3123_cov_2.790743"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PKJ43_03850"
     CDS             <1..212
                     /locus_tag="PKJ43_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71727.1"
                     /translation="AEFVTFIIPIAGILYVGSSFPYLRTREKTQSALMVPSSTMEKFL
                     YEFTERILLFFIMFPLYYFYLETWH"
     gene            212..610
                     /locus_tag="PKJ43_03855"
     CDS             212..610
                     /locus_tag="PKJ43_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71728.1"
                     /translation="MIVDTYNSAKGVVMNASPLSYGEMISDIEGQGKWVIIEGALFVL
                     SLAFAGAVTFRKYPLVKTVVFVGSVLAISAGYFVLIFEKLHLNHPWLENLGDHLSKTQ
                     GLTLSTFILLLLSLITLSFAYFKLKEKEVQ"
     gene            610..975
                     /locus_tag="PKJ43_03860"
     CDS             610..975
                     /locus_tag="PKJ43_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNZ71729.1"
                     /translation="MEFKDSKGIFQQIAESICDKIVSGEYPVGSKIPSVREHAAAVGV
                     NQNTIMRTYMELQRENIISNQRGIGFFVTESAPQQIINMRKEEFFNVHLPEFIRQVKL
                     LNLSKEELKPLFDQLNEKP"
     gene            972..1625
                     /locus_tag="PKJ43_03865"
     CDS             972..1625
                     /locus_tag="PKJ43_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71730.1"
                     /translation="MKTSKIIFITFFGVIGLFLLSLLIQVPQEKRGMKIETETEALPK
                     FSHLEITKGANLIVSYGSSDSIKLAHNKGVIVTKPVYWTKGDTLIVDPIPNQENFFLE
                     LTCSGLKSISLTESHMDIKQISLDNLRIEGTKAEIGFYDVTIDSLSMSLSSESRFWCS
                     NSKVKKVNLIADKSNADFSIDPIAELKAELHESSELSAGKVLQSNVTSDETSRYYSR"
     gene            complement(1681..2130)
                     /locus_tag="PKJ43_03870"
     CDS             complement(1681..2130)
                     /locus_tag="PKJ43_03870"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNZ71731.1"
                     /translation="MKRTLTLLALLFMLAVTNAYCQSSSLSIKKEKFNIDKQGEDNIG
                     YTQAIKVGNTIYISGSVGWGNMKDALKLAYDEIDKTLKYYNATFKNVVKENLYSTALD
                     SVIANKNIRLQYYGTDYPAATWVEVKRLYNEGLVVEIEVTAVLPEEK"
     gene            2829..>3123
                     /locus_tag="PKJ43_03875"
     CDS             2829..>3123
                     /locus_tag="PKJ43_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71732.1"
                     /translation="METTDQLNTSWRKVASTIYRKPVDSKIFGGVEFDVTELESFISQ
                     KRKEGIKITLTHFFVLAIARIVKEQIPEFNSYLRRGKIVARPTINPAVSVLKAD"
BASE COUNT          962 a          590 c          609 g          962 t
ORIGIN      
        1 gggcagaatt tgtaacattt ataattccga ttgccggaat actttatgtt gggtcttcgt
       61 ttccatatct gagaacacgg gagaaaacac aaagtgctct gatggtacct tcatcaacga
      121 tggaaaagtt tctatatgaa ttcacagaaa ggattttact tttcttcata atgttcccat
      181 tatactattt ctatttggaa acatggcatt aatgattgta gatacatata attcagcaaa
      241 aggagttgtc atgaatgctt caccattgtc ttatggtgaa atgatctctg atattgaagg
      301 ccagggcaaa tgggtaataa ttgaaggagc tttatttgtt ttatcgttgg catttgccgg
      361 tgctgttact ttccgtaaat atcctttggt aaaaacagtc gtttttgtag gttctgtcct
      421 tgccataagt gcaggttatt ttgtcctgat tttcgaaaag ttgcatctca atcatccatg
      481 gttggaaaat cttggtgacc acttgagtaa aacacaaggt ttgactttat caacttttat
      541 attgttactc ttaagcctga tcacactttc atttgcctat ttcaaactaa aggaaaagga
      601 ggtacagtga tggaattcaa agattcgaaa gggatttttc agcagatagc cgaaagcatt
      661 tgcgacaaaa tcgtatcggg cgaatacccg gtaggaagta aaataccctc ggtgcgcgaa
      721 catgccgctg ccgttggggt caatcaaaac accataatga gaacgtatat ggaattacaa
      781 cgcgaaaata tcatcagcaa tcagcgtggc attggttttt ttgtaaccga aagtgcgccg
      841 cagcaaatca tcaacatgcg caaagaagaa ttctttaatg tgcatttgcc cgagtttatc
      901 cgtcaggtaa agttgttgaa cctgagcaag gaagagctga aaccattgtt tgaccaatta
      961 aacgaaaagc catgaagaca agcaaaataa tattcatcac attctttggg gtaattggat
     1021 tgttccttct ttcgttgctg atacaggttc ctcaagaaaa acggggtatg aaaattgaaa
     1081 ctgagaccga agcccttcct aagttttcac atttagaaat taccaaaggg gcaaatctta
     1141 tagtgagtta tggatcaagt gattctataa aattagctca taataaaggg gttattgtta
     1201 caaagcctgt ttattggaca aaaggcgata cgctgattgt tgacccaatc ccaaatcagg
     1261 aaaacttctt tctggaactt acttgtagtg gtctaaaatc tatctcattg accgagagcc
     1321 atatggatat taaacaaatt tctcttgata atctacggat tgaaggaact aaggctgaga
     1381 taggtttcta tgatgttaca atcgactcat tgagtatgag tttatcatca gaatcacgat
     1441 tttggtgcag taattcaaag gtgaaaaaag taaatctaat tgctgacaaa tctaatgccg
     1501 atttcagtat tgacccaata gcagaactga aagccgaatt gcacgaaagc tctgaacttt
     1561 cggccgggaa ggttcttcaa agcaatgtaa cgtctgacga aacaagccgc tactattcgc
     1621 ggtaggctat aaaaagacaa tgtattgaaa gaaggtctga caagttcagg ccttctttgt
     1681 ttatttctct tcgggcagaa cggctgtcac ttcaatctcg accaccaaac cctcattgta
     1741 aagcctttta acctccaccc aagttgctgc cggatagtcg gttccataat actgtaacct
     1801 gatattttta ttggcaatta ccgaatcaag tgccgtgcta taaaggtttt cttttacaac
     1861 atttttgaac gtcgcgttat agtacttcaa cgttttgtcg atttcatcgt aagcaagttt
     1921 aagggcatct ttcatgtttc cccagccaac cgaccccgaa atgtaaatgg tatttccaac
     1981 tttaattgct tgtgtatacc caatgttgtc ctctccctgc ttgtctatgt tgaatttctc
     2041 tttcttaatc gacaaggaac tgctttgaca ataagcattt gtgacagcaa gcataaagag
     2101 tagtgccaat aaagtgagtg ttcgtttcat agttatattt taatttgatt aggttatatt
     2161 tttaatttat tcttctacaa aacagacaat caggcattga tattccctta ctgaaaggtt
     2221 gattttaact ttactcgagt tcactagttt gacaattttt acagtggacc agtgaataag
     2281 taagccatgg ttgcaatcaa atatttaaga aaaatatatt tcctggcatc gacactgttt
     2341 cctgatgaag tttatatata taccagatat acagatattt cacctccata ttttgagtgg
     2401 cacaaaccaa attttgtcaa ctaaacattg acatttatat gttgccatcg ttaatgctcc
     2461 gaaggagcaa tatctcaata accgtgtggc gtaagcccac ggtgtgtaat acaagaaagg
     2521 atcagccccg aatggggttg tatttgaaga agtttggaat aacaaaaact tgagccaatg
     2581 cttggtacaa cccctccggg gcagtgcctg ccttttctta acctccacgg gctaatgccc
     2641 gtggttattg aaataatacc gcttcgcagc ataaagtcag gaaatattta caaaaaactt
     2701 ctatttttgg ttttgtcttt ttattcataa cctattgcta gaaacggagc tgaattaggt
     2761 aaaaatacat ctataacagt ctcctttgct gcattgaaac acgaggaaag cctcaataat
     2821 tatttactat ggaaacaacc gaccagctga acacttcgtg gcgtaaagta gcctccacca
     2881 tttacaggaa accagtagat tcgaaaattt ttggcggagt agaatttgat gttaccgagt
     2941 tggaatcgtt tatcagccaa aaacgtaaag aaggtataaa aattaccctt actcactttt
     3001 ttgtgctggc cattgcgcga atagtaaaag agcaaattcc cgagttcaat tcataccttc
     3061 gcaggggtaa aattgtagcc cgtccaacga taaatcccgc ggttagcgtt cttaaggccg
     3121 acg
//
LOCUS       DAOPUW010000169         3122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_32022_length_3122_cov_3.368112, whole genome shotgun sequence.
ACCESSION   DAOPUW010000169 DAOPUW010000000
VERSION     DAOPUW010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32022_length_3122_cov_3.368112"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /locus_tag="PKJ43_03880"
     CDS             complement(<1..1453)
                     /locus_tag="PKJ43_03880"
                     /inference="COORDINATES: protein motif:HMM:NF023855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HNZ71733.1"
                     /translation="MSYLKFDKNQLVNLEYSLMREILHTNRAGSYCCTTLSGCNTRKY
                     HGLLVCPNNLLDGGKHVLLSSLDATIIQHGAEFNLGIHKYSGDFYEPRGHKYIRDFDI
                     ETIPITTYRVGGVILTMERVLVEEAEQVLIRYTLVDAHSETKLRFKPFLAFRNVHTLS
                     HSNLYANSKYKEVPKGVAFQLYDGYPSLNMQFSKNTEYVHNPDWYYNIEYMKDRTRGY
                     DYLEDLFVPGYFETDIKKGESIVFSASTSEARPEELKKLFIREVNNKTPRDSFFGCLR
                     NSAEQFVVKMPSFTDIQAGYPWYNGRINQTFVALPGLSLALNNPDLVSRIIDTNVSRL
                     KNGLFPRKWGRHGSDYDSVDSPLWFFWTIQNLVDDLGGKKAVWKKYKPAFTEILEAFK
                     KGTDFNIGMMENGLINAYTPGIALTWMNAYSDGFPVTPRYGSPVEVNALWYNAVCFAL
                     ELASEAKDKAFIKEWGELPEHIAASFISTFWSEE"
     gene            complement(1506..2516)
                     /locus_tag="PKJ43_03885"
     CDS             complement(1506..2516)
                     /locus_tag="PKJ43_03885"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HNZ71734.1"
                     /translation="MGVQRGDFALRTEVENVDLGFKLGYSSQSLFIGSCFSDNIGNIL
                     LRNKFPVMSNPFGVLYNPMSISKAIGRIISHQNIEKGELVFHNNLWHSFDFHGFFSGV
                     DPDTVVFRCNQAIDSAHRFLKTSRVLFITFGTSWVYRHLQQNVIVSNCHKIPDYEFDR
                     FRLSVDEIVNEWQGILFKIQKFNPDLKVVFTVSPVRHLKDGIHANQLSKAVLLLSIDE
                     IIRRSTPGLCSYFPAYEVVNDELRDYRFYDKDMIHLSETGVLYIFEIFKSVFFSSSTI
                     ECMKKVSSVVTAAEHKQLTEDISAIRQFATGMLRKIEILKIEYPEVNFDGEIKHFSEL
                     GA"
     gene            2731..3096
                     /locus_tag="PKJ43_03890"
     CDS             2731..3096
                     /locus_tag="PKJ43_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNZ71735.1"
                     /translation="MLTDTHSHIYSGEYNGEVDEVIKRALEQGVTKIMLPNIDSSSIK
                     MMLDLADSYPGICFPMIGIHPTSVKEDFKEELEVVEFWLSKRKFYAIGEVGIDLYWDK
                     TFREEQEYVFSRQIQLANS"
BASE COUNT          997 a          657 c          566 g          902 t
ORIGIN      
        1 tttcttcgct ccaaaatgtg ctgatgaacg aggctgcgat gtgctctggt aactcacccc
       61 attctttgat aaaagcctta tcttttgctt cagatgccag ctccaaggca aaacacactg
      121 cattatacca caaagcatta acttcaaccg gagaaccata acgcggagtt acaggaaaac
      181 cgtcggagta tgcattcatc caggtaagtg caatcccagg agtgtaagca ttgataagcc
      241 cgttctccat cataccaata ttgaaatcgg tgcccttttt aaatgcctct aaaatctcag
      301 taaatgcggg cttgtacttt ttccacacag ctttcttgcc tcctaagtcg tcgaccaaat
      361 tttgtattgt ccagaaaaac cacaacggac tgtcgacaga gtcataatcg gaaccatgcc
      421 taccccactt tctgggaaat aacccatttt taagcctcga cacatttgta tcaataatac
      481 gggaaaccaa atcggggtta ttcaaagcga gcgacaaccc gggcagtgca acaaatgttt
      541 gatttattcg cccgttatac caaggatatc ctgcctgtat gtcggtgaac gatggcatct
      601 taaccacgaa ttgctctgca gaattgcgaa ggcaaccgaa aaatgaatca cgcggagttt
      661 tgttattcac ttcccgtata aacagttttt tcagttcttc tggccgggct tcgcttgttg
      721 aagctgaaaa aacaatgctt tccccttttt taatatctgt ttcgaaataa ccaggaacaa
      781 aaagatcctc gagataatcg tacccccgag tccgatcttt catatattct atattgtagt
      841 accaatcggg attatgaaca tactctgtat tctttgaaaa ttgcatgttc aacgacggat
      901 acccatcgta taattggaaa gctactcctt tgggaacttc tttatatttt gaattggcat
      961 agaggtttga atgactcagc gtatgcacat tacgaaaagc taaaaatggt ttgaaccgga
     1021 gcttagtttc agaatgagca tcgactaacg tgtagcggat aagcacttgc tcggcctctt
     1081 caaccaaaac cctttccatt gtaagaataa caccaccaac acgatatgtg gtaataggaa
     1141 tggtttcaat atcgaaatca cggatatatt tatgcccccg gggttcataa aagtcgccgg
     1201 aatatttatg gattcctaag ttgaattcgg caccatgctg aataattgtt gcatcgagcg
     1261 atgaaagcaa tacatgcttg cccccgtcga gtaaattatt agggcatacc aataaaccat
     1321 gatacttacg ggtattgcat cctgaaagcg ttgtgcagca ataagaaccg gcacgattgg
     1381 tatgcaatat ttccctcatc aaggaatatt caaggttaac cagttgattt ttatcgaatt
     1441 ttaagtaact cattcctcgt taataattga ttttacacct tataaaatta aaaaaatagt
     1501 ttgtcttatg ccccaagttc gctgaaatgt ttgatttctc catcaaaatt aacctcagga
     1561 tactctattt taagtatttc tatcttgcgt aacattccag tagcaaattg cctgatagcc
     1621 gaaatatctt ctgttaattg tttatgttca gcagcagtta ccaccgatga aactttcttc
     1681 atacattcaa tggtcgatga agagaaaaat accgatttga atatctcgaa aatgtagaga
     1741 accccggttt cagaaagatg aatcatatcc ttgtcataaa accgataatc gcgaagctca
     1801 tcattaacaa cttcataggc aggaaaatat gaacacaagc ccggggtact tctcctgatt
     1861 atttcgtcaa ttgaaagtag aagtacggct ttactcagct gattggcatg tataccatct
     1921 ttaaggtgtc tcaccgggct aacggtaaaa acaaccttca agtctgggtt aaatttttgt
     1981 attttgaaca aaattccttg ccattcattc actatctcat caacggataa acgaaaccga
     2041 tcgaattcat aatcggggat tttatgacaa ttggaaacaa taacattctg ctgcagatgg
     2101 cggtaaaccc atgatgttcc gaaagtaatg aataaaacac gtgacgtttt cagaaaccga
     2161 tgcgctgagt caatagcctg attgcaacga aaaacaaccg tgtcggggtc tactccggag
     2221 aaaaatccat gaaaatcaaa cgaatgccaa agattattgt gaaaaaccaa ctctcctttt
     2281 tcgatatttt gatgactaat aattcgccca atagccttag aaattgacat cggattgtaa
     2341 agtacgccaa aaggattaga catgaccggg aatttattcc gcagaagaat gttcccaata
     2401 ttgtcggaaa aacatgaacc aataaatagg gactgagaag aatatcccaa cttaaaccca
     2461 agatctacat tttcaacctc agtccgcaat gcaaaatcac ctctttgtac gcccataaca
     2521 gttcttagaa tttaccttca tggcaatata tcaaaaaatt gtaaaattga agtcgacgaa
     2581 aaaaataatt atttatccgg ctaacttgct gtattcgttg tctattcatt ttcaacatct
     2641 tcaatttaca ataatataac gtaatatttt tggagagttt agcaccaacc atcacttaat
     2701 ttttattttt gccgcaaatt ttgaacaacg atgctcactg atactcattc acatatctat
     2761 tcaggagaat acaacggcga ggtcgacgag gtaataaaac gggcgttgga acaaggcgta
     2821 acaaaaatta tgctccccaa catcgacagt tcatcaatta aaatgatgct tgacttagcc
     2881 gacagctatc ctggaatatg tttcccgatg atcggtattc atcctacctc ggtaaaagaa
     2941 gatttcaaag aagaactcga ggtggttgaa ttctggcttt cgaaaaggaa attttatgcc
     3001 attggcgagg ttggcatcga tttgtattgg gacaaaactt tccgcgaaga acaggaatac
     3061 gttttttccc gtcaaattca actggcaaac tcctgaaaat cctatcggtt ttgacataat
     3121 aa
//
LOCUS       DAOPUW010000170         3107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_32257_length_3107_cov_2.877457, whole genome shotgun sequence.
ACCESSION   DAOPUW010000170 DAOPUW010000000
VERSION     DAOPUW010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32257_length_3107_cov_2.877457"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PKJ43_03895"
     CDS             <1..412
                     /locus_tag="PKJ43_03895"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNZ71736.1"
                     /translation="RAMEKINGNRTGEIPIDKLTEIPESDTIERVVVKDKNKIHIIPT
                     EQIRYIESLDDYVMIYTRDKRFVKQKTMKFFEDHLNPKEFIRIHRSYIVKVEEIAEIQ
                     QYEKESHIVILKNDHTKLKVSKTGYKNIKEALNF"
     gene            complement(539..1840)
                     /locus_tag="PKJ43_03900"
     CDS             complement(539..1840)
                     /locus_tag="PKJ43_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ71737.1"
                     /translation="METNTLKSKLNEIKDSVLNRVLIVIMFVMVLGITFSLLRIPQTG
                     FLFTYGVQLFLALVIASLYIFRNKLSSNLKGAIFLSVMLILALSGLLSFGLYGFGYTY
                     FIPASAVAFIYFKRRTGWAVTLSSLFVLVVIAVFFNRGILHFSPQKPDYMESASMWLN
                     MIITVTLIASVITMFWSNLFSLLSNTLTHINHQQEDMMRMNEQLILARDKALESDRLK
                     SAFLANISHEIRTPLNIIIGFSDMLSQAETQDEKEEYSQVIKHNSAMMLKMVNDIVDF
                     SKIETNSLALNQTKFKVADVIREVEKKVLWKKPELVEWTCNAENSELVTDKDRFGQVV
                     LNLADNALKFTSEGRVEISCSTEGGKIVCKVADTGIGISGDDESRIFERFYKVDTFVP
                     GAGLGLSISKSIANMMGGDILVHSEQGKGSVFEFWIPCHSN"
     gene            1918..2310
                     /locus_tag="PKJ43_03905"
     CDS             1918..2310
                     /locus_tag="PKJ43_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71738.1"
                     /translation="MEILFVIILILILTAGVVFIVLHKKKKEVEEPDVTPGTMPESDC
                     CGAHEVCERDLVKVNPDIIEYFDDEDLDKFSNINENEYSDDQIDEFREILYTLKKSEI
                     QSWILSLSRRNIALPTILHDEARMLILE"
     gene            complement(2337..2795)
                     /locus_tag="PKJ43_03910"
     CDS             complement(2337..2795)
                     /locus_tag="PKJ43_03910"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HNZ71739.1"
                     /translation="MIQRIQTIFLSIALLLMGILFFIPLAEVAVGEKLYAFGIKGLVD
                     SQSGNTTYAAWALFVYLLFVVCLQAFIIFSFKKRVLQMRLATFNLILMLGFVLLGWLF
                     VKFSLSALGEGVYSLKLPLVFPFASAVLNYLAIRAILKDELLIRSIDRIR"
     gene            complement(2919..>3107)
                     /locus_tag="PKJ43_03915"
     CDS             complement(2919..>3107)
                     /locus_tag="PKJ43_03915"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNZ71740.1"
                     /translation="GSVSRSQNIEVSYLDSMGKKQREEINGFVAVIFQHEIDHLSGVM
                     YFDHVFGGYDALLPYNEE"
BASE COUNT         1070 a          660 c          560 g          817 t
ORIGIN      
        1 aagggcaatg gaaaaaatca acggtaacag aactggcgaa atacccatcg acaagctgac
       61 cgagataccc gaaagcgata ctattgagcg cgtagtggta aaagacaaaa ataaaattca
      121 catcatccct actgaacaaa taaggtacat cgagtcgctc gacgattatg tgatgattta
      181 cacgcgcgac aagcgttttg taaaacaaaa aaccatgaaa tttttcgaag atcacctgaa
      241 cccgaaagaa ttcatccgca tccatcgttc ttatattgtt aaggttgaag agatcgccga
      301 aattcagcaa tatgaaaaag aatcgcacat cgtgattttg aaaaacgacc atacaaaact
      361 gaaagtgagc aaaacagggt acaaaaacat caaagaagca ctaaatttct aattgggaag
      421 acccaattca cgtagactta tagcaatcaa acaaataaaa aagcagctat ctcaggctgc
      481 tttttcattt atatcaaact gtacttcttt acttgatttg tgcattttac aaggatgatc
      541 aatttgaatg gcagggaatc cagaactcaa acactgaacc cttcccttgc tctgaatgaa
      601 caagaatatc gccacccatc atatttgcaa tcgacttact gatactgagc cccaaccctg
      661 ctccgggaac aaaagtatcg actttgtaaa atcgctcgaa aatcctcgat tcgtcatcac
      721 cagaaatacc aatacccgtg tcggcaacct tgcagacaat ttttcctcct tcagtagaac
      781 aacttatttc aaccctacct tccgatgtaa atttaagggc attgtcggcc aagtttagca
      841 caacttgccc aaagcggtct ttatcggtaa ccaattcact gttttctgcg ttgcaagtcc
      901 actcgaccaa ctctggtttt ttccagagga ccttcttttc cacttcgcga ataacatcgg
      961 ctaccttgaa ttttgtttgg ttgagcgcta atgaattcgt ttcaatcttt gaaaaatcta
     1021 caatatcatt taccattttc agcatcatgg cgctattgtg ttttataacc tggctatatt
     1081 cctctttttc atcttgagtt tcagcctgag aaagcatatc agaaaaaccg ataattatat
     1141 tcaacggagt gcgtatttcg tggctgatat tggcaagaaa agctgatttc aaacggtcag
     1201 attcgagggc tttatcacgt gcaagaatga gctgttcatt catgcgcatc atgtcttcct
     1261 gctgatggtt aatatgcgta agcgtgttcg acaataaact aaacagatta ctccaaaaca
     1321 tggtaatcac tgaggcaata agggtaactg ttattatcat gttcagccac atagaagcag
     1381 attccatata atcaggtttt tggggagaaa agtgaaggat gccccgattg aaaaacaccg
     1441 caattacaac caaaacaaat aaagaggaca aggtgacagc ccaccctgta cgccgtttaa
     1501 aataaataaa agctactgcc gatgccggaa taaaatacgt gtacccgaaa ccatacaagc
     1561 caaacgacaa caagccgcta agggcaagta tcagcataac agacaagaaa atagctcctt
     1621 ttaggttcga cgaaagctta tttcggaaaa tatagagcga agcaataacc aacgccaaaa
     1681 atagttgaac accataggta aacaaaaaac cagtttgggg aattcgcaac aacgaaaagg
     1741 taatgcccaa caccattaca aacataataa cgatgagtac acgatttaga actgagtctt
     1801 ttatttcatt gagttttgat tttagagtat tagtttccat gttatcgatt gtttttattt
     1861 cccagaaagg tatggcattt ttcaaagaaa tcaatcgaat aatcctaatt ttgaaccatg
     1921 gaaatactat tcgttattat actgatatta atcctcaccg ccggagttgt ctttattgtt
     1981 ctccataaga agaagaaaga ggttgaagaa ccagacgtta cacctggaac catgcccgaa
     2041 tccgactgtt gtggtgcgca tgaagtgtgc gagcgcgatt tggtaaaagt caatcccgac
     2101 atcatcgagt actttgacga tgaagatctc gataagttca gtaacattaa cgaaaatgaa
     2161 tattcagacg atcaaatcga cgaatttcgc gaaatccttt acactttaaa aaagagtgaa
     2221 atacaaagtt ggatactcag cttgtcgcga cgcaacatcg ccctccccac tattttgcac
     2281 gacgaagccc gaatgctgat tcttgaataa gtaaaggttg agaaacccag tatagcttat
     2341 cgaatcctgt cgatcgaacg gatcaacaat tcatctttta gtattgcccg aattgccaaa
     2401 taattcaaca cagcagatgc aaaaggaaat accaacggaa gcttcagtga ataaacccct
     2461 tcgcccaacg ccgaaagcga gaattttaca aacaaccagc ctaacaacac aaaacccaac
     2521 ataaggatca gattgaaagt ggccaatctc atttgcagca cacgcttttt aaagctgaaa
     2581 ataataaagg cctgaagaca tactacaaac aataaataaa caaacaaagc ccaagcagcg
     2641 taagttgtgt tgcccgactg agaatcgaca agtcccttaa tgccaaaagc atacaatttt
     2701 tctccaactg caacttcggc tagcgggata aaaaataaga tacccataag cagtagggca
     2761 atcgacaaga atatggtttg aatacgttgt atcatgatta attttttcgg acaaaaaaaa
     2821 tagattctgg cgagtcttca agcagttttt actcttcgtt acaagaattg cgattatgaa
     2881 attaggttag aagattattt acaaaagaaa agatttgctt attcttcatt atatggcagc
     2941 agggcatcgt agcccccgaa tacatgatca aaatacataa ccccgctcaa atgatcgatt
     3001 tcgtgttgga agattaccgc aacaaaacca tttatttctt cgcgttgctt ttttcccatt
     3061 gagtcgagat aagacacctc aatattttga gaccggctta cacttcc
//
LOCUS       DAOPUW010000171         3104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_32293_length_3104_cov_2.374549, whole genome shotgun sequence.
ACCESSION   DAOPUW010000171 DAOPUW010000000
VERSION     DAOPUW010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32293_length_3104_cov_2.374549"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PKJ43_03920"
     CDS             <1..536
                     /locus_tag="PKJ43_03920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ71741.1"
                     /translation="TTLHESNIACFNALDTVIVAGDETNVILESASSVEIIAGKTIRF
                     MPGFHAKEGSNLFGHITTNNTFCLYEKSPGIEHFETEKSEPISIAADTSTIITITEKQ
                     AKIYPNPSNGQFIVDMNQFEGISKIMITTTSGVVVYKAQTEDKTIQIELNHLSKGLYF
                     VNIENRKYHQCNKIIIR"
     gene            750..2765
                     /locus_tag="PKJ43_03925"
     CDS             750..2765
                     /locus_tag="PKJ43_03925"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNZ71742.1"
                     /translation="MNNAIVNKAADNIRILAASMVEKAKSGHPGGAMGGADFINVLYS
                     EFLNYDPSDMSWINRDRFFLDPGHMSPMLYAQLALIGKFSMEDCANFRQWGSVTPGHP
                     EVDVERGVENTSGPLGQGHVMAIGAAIAERFLVARFGEWMAHKTYTFISDGGVQEEIS
                     QGAGRLAGHLGLNNLIMFYDSNDIQLSTETHEVTGENTEMKYKAWGWNVMTINGNNAD
                     EIRAALKAAQNEKDRPTLIIGKTIMGKGAVKEDGSNYERKCATHGMPLGEAGASYEKS
                     IENLGGDPKNPFKIFPEVQELYAKRREELIKAAALKKAEQAKWEAENPELAAKLKKFF
                     SKEAPAFDYSKIEQKPGQATRAASATVLASFAEQVDNMVVASADLSNSDKTDGFLKKT
                     KAFKKGDFSGKFLQAGVAELTMACVMNGMALHGGIIPACGTFFVFSDYMKPAARMAAL
                     QELPVKYVWTHDAFRVGEDGPTHQPVEQEAQIRLMEKLQNHKHKNSMLVIRPADVNET
                     TVAWKMAMENTDTPTALILSRQNIKDVTTYETALNATKGAYILKDSEGTPDIILLASG
                     SEVSTLVDGAELLEKEGIKCRIVSVPSEGLFRSQPKEYQNSVLPCGVKKFGLTAGLPV
                     NLEGLVGADGKVFGLPSFGFSASYKVINEKLGFNGENVYKQVKEILA"
     gene            complement(2842..>3104)
                     /locus_tag="PKJ43_03930"
     CDS             complement(2842..>3104)
                     /locus_tag="PKJ43_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71743.1"
                     /translation="KVLVTFNVPNTHREIIENYLNENNLFADEPTMSVGKTYTVYNIQ
                     VDTSGSKVPLAMVRYQLAKLGATNIDTLPIQSSIQSLEAIKF"
BASE COUNT          999 a          651 c          643 g          811 t
ORIGIN      
        1 acaccacact gcacgaatcc aacatcgctt gttttaatgc tctcgacaca gtaatcgtag
       61 caggggatga gacaaatgtc atcctcgaaa gtgcatcatc tgttgaaata attgctggca
      121 aaacaatacg gtttatgcca ggatttcatg caaaagaagg cagtaattta tttggccata
      181 ttaccaccaa taatacgttt tgcctttatg agaaaagccc gggcatagaa catttcgaaa
      241 ctgaaaaaag tgagccaatc agtatcgccg ccgatacttc aacaataata accattactg
      301 aaaagcaggc taagatttat cctaacccca gtaacggtca gtttattgtc gatatgaacc
      361 agtttgaagg catttcaaaa ataatgatta ccaccacttc aggagttgtt gtttacaaag
      421 cccaaaccga ggataaaacg attcagatcg agttgaacca cctaagcaaa gggctctatt
      481 ttgttaatat cgaaaacagg aaataccatc aatgcaataa aataattata cggtaggatt
      541 cagtttggaa taaaaagcaa taaaacaaat tacaacagga gcaagagcca atcaacaatc
      601 agattgtgtt aattctctgt caagaaaata aaaaccaaag caaaggtttg aacaaggctt
      661 ttttaggttg ctgaatagtt ttatatttgc tctccgtttc aatataagta ataataacaa
      721 ttacctaagg tattaaatta taacgacaaa tgaacaatgc aattgtaaac aaagctgccg
      781 ataacattcg tattttggcc gcatcgatgg tcgaaaaagc caagtcgggg catcccggtg
      841 gcgctatggg tggcgccgat ttcatcaacg tactctattc agaattcctc aactatgacc
      901 ccagtgatat gagctggata aaccgtgacc gttttttcct cgatccaggt catatgtcgc
      961 caatgttata tgcacaactt gcccttattg gcaaattcag catggaagac tgtgccaact
     1021 tccgccaatg gggaagcgta acacccgggc atcccgaagt tgatgttgaa cgcggagttg
     1081 aaaacacatc gggacctctt ggtcaaggcc acgttatggc tataggcgct gcaattgcag
     1141 aacggttttt agttgcccgc tttggtgaat ggatggctca caaaacctat acatttatct
     1201 ccgacggtgg tgttcaggaa gaaatctcac aaggtgcagg ccgtctggca ggtcaccttg
     1261 ggcttaacaa cctgatcatg ttctacgact cgaacgatat ccaactttcg acagaaaccc
     1321 atgaagtaac cggcgaaaat accgaaatga aatacaaagc atggggctgg aatgtaatga
     1381 ctatcaacgg aaataatgca gacgaaattc gcgctgcact gaaagccgca caaaacgaaa
     1441 aagacaggcc aaccctcatc attggtaaaa caattatggg taaaggggcc gttaaagaag
     1501 atggctcgaa ttacgaacgc aaatgtgcaa cccatggtat gccattgggc gaagcaggtg
     1561 catcgtacga gaaatcaatc gaaaacttgg gtggcgatcc taaaaaccca ttcaaaattt
     1621 tccctgaagt acaggaatta tacgccaaac gccgtgaaga attaattaag gccgctgccc
     1681 taaaaaaagc agaacaagca aaatgggaag ctgaaaatcc tgaattagct gcaaaattga
     1741 aaaaattctt ctcgaaagaa gctccagctt ttgattatag caaaattgaa caaaaaccag
     1801 gtcaggctac ccgtgctgca tcagctactg ttttagcttc ttttgcagaa caagttgaca
     1861 atatggttgt tgcatctgcc gacctgtcaa attctgacaa aacagatggt ttcctcaaga
     1921 aaacaaaagc gttcaaaaaa ggtgatttct ctggcaaatt ccttcaagct ggcgttgccg
     1981 agctcaccat ggcatgcgta atgaacggta tggctttaca tggcggtatt atccctgctt
     2041 gcggaacctt cttcgttttc agcgattata tgaaacccgc tgcccgtatg gctgcattac
     2101 aggaattacc tgttaaatac gtttggacac acgatgcatt tcgcgttgga gaagatggcc
     2161 caacccacca gccagttgag caagaagctc aaatccgcct gatggaaaaa ctccaaaacc
     2221 ataaacacaa aaactcgatg cttgtgattc gtcctgccga tgttaacgaa acaacagtgg
     2281 catggaaaat ggctatggaa aacaccgaca cgcctactgc actaatcctt tcccgtcaga
     2341 acattaagga tgtaaccaca tatgaaacag cactcaatgc aacaaaaggt gcgtatatcc
     2401 tgaaagattc agaaggtaca cccgacatta ttttgctggc tagtggttct gaagtaagca
     2461 ctttggtcga tggagctgaa ttgcttgaga aagaaggcat caaatgccgc attgtttctg
     2521 ttccttcaga aggtttgttc aggtcacaac ctaaagaata ccagaattcg gtactgcctt
     2581 gtggcgttaa gaaatttggt ctgacagctg gtttgccagt taaccttgaa ggacttgttg
     2641 gtgccgacgg taaagtattt ggtttgccat cgttcggttt ttctgcttcg tacaaagtta
     2701 tcaatgaaaa acttggtttc aatggcgaaa atgtatacaa acaagtgaaa gaaatccttg
     2761 cctaatacca attcttacaa taaaattaaa agagccatcg ttttgatggc tcttttaatt
     2821 tttataataa tcatgctcat tctaaaactt tattgcctca agactttgaa ttgaagactg
     2881 aatagggagc gtatcgatat ttgttgcacc aagcttggcc aactggtaac gtaccatggc
     2941 caaaggaact ttgctacctg aagtatctac ctgaatgttg tataccgtat aggttttacc
     3001 cacactcatg gttggctcgt cggcaaaaag attgttctcg tttagataat tctcgattat
     3061 ttcgcgatgt gtattaggta cattgaatgt taccaacacc ttgt
//
LOCUS       DAOPUW010000172         3099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_32361_length_3099_cov_3.468463, whole genome shotgun sequence.
ACCESSION   DAOPUW010000172 DAOPUW010000000
VERSION     DAOPUW010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32361_length_3099_cov_3.468463"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="PKJ43_03935"
     CDS             complement(<1..927)
                     /locus_tag="PKJ43_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71744.1"
                     /translation="MNTKAFRSTLFLAFWLLCPWAFSQPANTPVGKLNAVLEQAKNTF
                     DKDKVFVKTDKDIYSPGEKIWFYAQVYNALTQQLSSAAELIVMLKGENGEVIGDSRYV
                     LINGQSESWFQVPSWSPEGNVYLVAYTSAAIRANDASLAAVKPLIINTYRKNDFIIDA
                     WLNKEIYSPGEEVKLSVKLTSLTPGHKKEKLEIALYDRTTKKYPQKFTLAVNQVNELK
                     IKLPSKVYNCLSLTLGLAGKGSILHKIPLRTTDDNISVEFYPEGGVLLANNIQRILYR
                     ATDPFGMPIDIEGNVFDQFGNQAGAGKIVKKGY"
     gene            complement(939..2468)
                     /locus_tag="PKJ43_03940"
     CDS             complement(939..2468)
                     /locus_tag="PKJ43_03940"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNZ71745.1"
                     /translation="MRIYAALAVLMVMLVKVSFAQQNIGLLSSEVRLPLTPVVKADLL
                     DSIEKRLGIHFSYNPEWIGATDTVSFSTEKVVLLDLLSQLTDSSTTGFHLLDNQLVFY
                     PLKSDDVSRPAIPVDPFVVVGGMVTDAKDKSPVPYCNIAVTGKGLGAMSNNDGKFLIK
                     IPASCINDTLRFSCMGYMPYFIPVAEAKDSQLAVTLSKKIFNLKTIDVVHYQPSVLLQ
                     KYFDNVRKNYENEYNLFTTFYREIVREDNDFTDVSEAVLHVLKAPYSSVVREDMVKFL
                     KGRKSSNVRPFNDIRFKLRGGPYYITKLDVVKNEESFINPDFISLFNYSFERKTLLAG
                     RETAIVAFSPVYNLRELLYEGLLYFDIETGALARAEFNYTRQGLKEARRLMIEKEPRN
                     HKAIPAELSYMVEYTFVQGKWYLLSAQSSMEIKIIDREKRQKTNFYSTAEMLVTSIEK
                     GDLLHFSRKEIFRPGEFFTEKITSYDEQFWSDYNVIEPEEDLQKAVKNFDDHNLIIST
                     D"
     gene            complement(2557..>3099)
                     /locus_tag="PKJ43_03945"
     CDS             complement(2557..>3099)
                     /locus_tag="PKJ43_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNZ71746.1"
                     /translation="ESNVFTFLGVGLGGDLYGHKGRISALWIGGRFLDLPIVSYPESG
                     FINQVIEQENRNGSIGGEILRRFTTIFDYKNKMLVLRPNGDFNDGFYYNMSGLEIINP
                     VPGIPVFTISNVMEKSPAWEAGIRENDQVLSVNSNDHRNLTLNDINLMLRQRQDKKIT
                     MILLRNGQKIKTSFHLKESF"
BASE COUNT          904 a          694 c          624 g          877 t
ORIGIN      
        1 gtaccctttt tttacgattt tacctgcccc tgcctgatta ccaaattggt cgaaaacatt
       61 accttctatg tcgataggca tgccgaacgg atcggttgcc cggtacaaaa tacgctgtat
      121 attgttggcc aacagcaccc caccctcagg gtaaaattca acagaaatat tatcgtcggt
      181 tgtacgaagg ggaattttat gcaaaatgct tcctttcccg gcaagcccca gcgttaaact
      241 aaggcagtta tacaccttcg aaggaagttt aattttcaac tcgttaacct gattaacagc
      301 aagtgtaaat ttttggggat acttttttgt agtacggtcg taaagggcaa tctccaattt
      361 ttccttttta tggccgggag ttagtgaggt gagtttaacc gacaatttta cctcttcgcc
      421 aggggaatag atttctttat taagccatgc atcaataata aaatcgttct tacgataagt
      481 attaatgata agaggcttaa ctgcggctaa cgaagcatcg ttggcacgaa tggccgctga
      541 tgtgtaagcg accaaataga cattcccctc aggagaccat gaaggaacct ggaaccaact
      601 ttcactttgt ccgttaataa gcacgtaccg actgtcacca atcacttcgc cattttcacc
      661 tttcaacatg acaataagtt cagcagccga agagagttgt tgggttaacg cgttgtaaac
      721 ctgagcatag aaccatattt tttctcccgg cgaatagatg tctttatcag tcttaacaaa
      781 caccttatct ttatcgaagg tatttttagc ctgttcgagt accgcattta gtttccctac
      841 cggggtattc gccggttgcg aaaaggccca ggggcataac agccaaaagg caagaaaaag
      901 tgtactacgg aaagctttgg tattcataac aaaatcgttt aatctgttga aataattaaa
      961 ttgtggtcgt cgaaattttt cacggctttt tgcaagtcct cttccggctc aattacatta
     1021 taatcggacc aaaactgttc atcgtaactg gttattttct cggtaaagaa ttcaccgggg
     1081 cgaaagatct ctttccggct aaaatggagt aaatctcctt tctcgatcga tgtaacaagc
     1141 atttcagcag tactgtaaaa gtttgttttc tgcctcttct cccgatcaat gattttaatt
     1201 tccatggatg attgtgccga caagaggtac catttcccct gcacaaaagt atattcaacc
     1261 atgtacgaca actcggcagg aatggcttta tggttacgtg gctctttctc tatcatgagt
     1321 cgccgagcct cttttaaacc ctgacgggtg tagttaaatt cggccctggc cagtgcaccg
     1381 gtttcaatat cgaaatagag caacccttcg taaagcaatt cacgcaggtt ataaaccggc
     1441 gaaaaagcca caatagccgt ttcacgccct gcaagcaagg ttttacgctc gaaactgtaa
     1501 ttaaataaac taataaaatc agggttaatg aaactttctt cgtttttcac tacatcgagc
     1561 ttagtgatat aatatgggcc accgcgcaac ttaaaacgta tgtcattaaa aggccgcaca
     1621 ttagagcttt ttctcccttt caggaacttc accatatctt cgcgtacaac gctcgaatac
     1681 ggagccttga gcacatgaag cacagcttcg gaaacatcag taaaatcatt gtcttcgcgt
     1741 acaatctcgc gataaaatgt agtaaacagg ttatactcgt tctcgtagtt tttcctcaca
     1801 ttatcgaaat atttctgcaa caaaaccgac ggctgataat gaacaacatc gatcgttttc
     1861 aggttgaaaa tctttttcga cagggtgaca gcaagctgcg agtctttggc ctcggccaca
     1921 ggaataaaat acggcatata gcccatgcac gaaaaacgca aggtatcgtt gatgcatgag
     1981 gcaggaatct taataaggaa ttttccgtcg ttgttcgaca tagctcccaa gccttttccc
     2041 gtcacggcaa tattacaata agggaccggg cttttgtctt tggcatcggt aaccataccc
     2101 cctaccacta caaatgggtc gacaggaata gcaggcctgc tcacgtcatc agatttcaat
     2161 ggatagaata ccaactgatt atctagcaga tgaaaacccg tggttgagct atcggtgagt
     2221 tgtgaaagca ggtcaagcaa aaccactttc tcagttgaaa aagaaaccgt atcggtagca
     2281 ccaatccatt cggggttata ggaaaaatga atgccaagcc gcttttcgat actatcgaga
     2341 agatcggctt ttaccacggg agtcaatggc agccgcactt cagaagagag cagaccaatg
     2401 ttttgctggg caaaagaaac cttgaccagc attaccatca aaactgcaag agcggcatat
     2461 attctcatct cattatgtca aatttccttt gggcattcaa cgaagtcgaa aactgttctt
     2521 agatcagatt gggcaagaaa catcgaactt ccaacgttaa aagctttctt tcaaatgaaa
     2581 cgaagtttta attttttgtc catttctcaa aaggatcatg gttatttttt tatcctgtcg
     2641 ttggcggagc atcaggttaa tgtcgttgag ggttaaattc cggtggtcgt tggaatttac
     2701 tgatagcacc tgatcgtttt cgcggatacc agcttcccat gccggcgatt tttccatcac
     2761 attactaatg gtgaaaacag gtattccggg cacaggattt atgatttcga gcccactcat
     2821 gttgtagtaa aatccatcgt tgaagtcgcc attcggcctc aataccagca ttttattttt
     2881 atagtcgaaa atagtggtga aacggcgcaa aatctcgcca ccaatagaac cattccgatt
     2941 ttcctgttca ataacttggt ttataaaacc cgattcggga taagatacga taggcaaatc
     3001 gaggaaacgt ccaccaatcc acaaagcgct gattcggcct ttatgtccgt aaaggtctcc
     3061 cccaagccct acaccaagaa aagtaaagac attagattc
//
LOCUS       DAOPUW010000173         3078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_32667_length_3078_cov_2.658948, whole genome shotgun sequence.
ACCESSION   DAOPUW010000173 DAOPUW010000000
VERSION     DAOPUW010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32667_length_3078_cov_2.658948"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PKJ43_03950"
     CDS             complement(<1..535)
                     /locus_tag="PKJ43_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNZ71747.1"
                     /translation="MSENIGHECGVAMIRLLKPLEFYQQKYGSWQYGLNKLYLLMEKQ
                     HNRGQDGAGLVSLKLNSVPGEEYLSRSRSVKENPIKDIFDGIFRYFSSSAEKKPDEFH
                     DSNWAKKNLDFVGEAYLGHLRYGTFGRNSIEFVHPVMRQNNWKSRNLVLAGNFNLTNT
                     DELFNKLISLGQHPSAYT"
     gene            1172..2335
                     /locus_tag="PKJ43_03955"
     CDS             1172..2335
                     /locus_tag="PKJ43_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71748.1"
                     /translation="MNKNVAGIAALSVFLFGSVSTGFAQGVEGPNISSILEIEQYGRS
                     IHIMAMLLIGFGFLMVFVKKYGRSALTATFLLVSVSLPTYLAIKGLGVFEEKGEIEQF
                     ILAEFGAASLLIATGAVLGRIKMHQYLILGLLFIPFYMFNELVVIDDYFHFVGRVADT
                     GGSIVIHAFGAIFGISAAIFLTTKEQREIPIHADATSDRFSLLGSMVLWVFWPSFCAA
                     LVPNEAIPATVVNVFLALCGSTLATYFASVKIRGKISPADIANAALAGGVAIGSTCDY
                     ANHVEAMIIGVIAGTISTIGFAILQEKQQKFQKIVDTCGVTNLHGIPGLFGGLVALVI
                     VDGIDAGAQLKGILITAIIAIIAGLFTGKIVSLLGQPKLIYDDKAEFETEVSE"
     gene            complement(2517..>3078)
                     /locus_tag="PKJ43_03960"
     CDS             complement(2517..>3078)
                     /locus_tag="PKJ43_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNZ71749.1"
                     /translation="VLSGEGADEIFGGYLYFHKAPNAEEFHKETVRKIDKLHLYDCLR
                     ANKSLAAWGIEGRVPFLDKEFMDVAMRLNPEAKMAKDGKMEKWILRKAFEDFLPESVA
                     WRQKEQFSDGVGYSWIDTLRDLASKNISDEEIANAAERFPINPTMNKEEYHYRTIFHE
                     LFPSDSAASCVPSVPSVACSTPIALE"
BASE COUNT          810 a          550 c          637 g         1081 t
ORIGIN      
        1 cggtatatgc gcttgggtgt tgtcccaatg aaatcagttt gttgaatagc tcgtcggtat
       61 tggttaggtt gaagtttccg gccaggacaa gatttcttga tttccagttg ttttgacgca
      121 taaccgggtg cacaaattct attgaatttc gtccaaaagt accgtatcga aggtgtccaa
      181 ggtaagcttc gccaacaaaa tcgaggttct tttttgccca gttagaatcg tggaattcgt
      241 caggtttttt ttcggcactt gatgagaaat aacgaaaaat cccatcaaaa atatctttga
      301 taggattttc tttaaccgag cgcgagcgcg aaaggtattc ttcgcccgga acactgttca
      361 attttaatga aactaaccct gccccatctt gaccgcggtt gtgctgtttt tccattaaca
      421 agtataactt gttaagtccg tactgccacg atccgtattt ttgttggtaa aattcaagtg
      481 gtttcagtag tcggatcatt gcaaccccgc attcatgtcc aatattttcg ctcatattat
      541 tttatgtcaa ttattgaatt ctatttcttt caatttgtca ctgttttaat ttgaaataat
      601 cgttaaatga ttttttaatg atttatatcc gttaaaaaat tctagcaata ctggtataga
      661 actaacattt tgttttttat ctggggtgaa tttagtgcaa gattgtgctt gttggacatt
      721 tgctgttcgc aatatttgtt tgggttgata ggttattgtg ttgtttttta gcgattaaca
      781 tgaaatgatg tttttctttt ctacgatatg aatttagtgg attttcaata aaaaaattgt
      841 gttttttagt atgaaatgtt catgtgtgcc aaagcttgca ccaaactaaa atggacagat
      901 ggctatttta tcctatctaa gcttaaggaa tagagttttt tgcgtgggtt gtagctattt
      961 gctttgaaat catgtactaa taaaactagt caacaactat tagattagat tcgtttctgt
     1021 atgttttccc gggttaggtg tcattaaatt taggtttttg atgtcaaaat gattcatttc
     1081 tataagtatt tcacgcaaaa aagttttaac aatcagatac cattcgctaa acattacttt
     1141 tggcaacctc taatcaattc ttaaaactat tatgaataaa aatgttgctg gaattgctgc
     1201 tttatcagta ttccttttcg gttctgtttc aactggattt gcgcaaggtg ttgagggacc
     1261 aaacatctct tcgatattgg aaattgaaca gtatggtcga tccattcaca ttatggcaat
     1321 gttgctgata ggctttggtt ttttaatggt atttgtaaag aagtatggac gttcggcatt
     1381 aacggcaact tttcttcttg taagtgtttc gttaccaact tatcttgcaa ttaaaggact
     1441 tggcgttttt gaagaaaaag gagaaattga acaatttatt ttggctgagt ttggtgcagc
     1501 aagtttgttg attgcaaccg gtgctgtttt gggccggata aaaatgcatc aatacctgat
     1561 tcttggcctg ctttttattc ccttttacat gtttaatgaa cttgtcgtaa tcgacgatta
     1621 ctttcatttt gttggccgtg ttgccgatac tggtggatct attgttattc atgcatttgg
     1681 agcaattttt ggaatttcgg cagccatttt cctaacaact aaggaacaac gtgaaattcc
     1741 tattcatgct gatgctacaa gtgatcggtt ttcgttgttg ggaagtatgg ttttatgggt
     1801 gttttggcca agtttctgtg ccgcgttagt tccaaatgaa gccatcccgg cgacggtggt
     1861 aaatgtattt ctggctcttt gcggttctac gctcgctact tactttgctt ctgtgaaaat
     1921 cagagggaaa attagccctg ctgatattgc caatgcggct cttgcaggtg gcgtggcaat
     1981 tggatcaact tgcgattatg caaatcatgt tgaagcaatg attattggtg taattgcagg
     2041 aacaatttcg accattggct ttgctatttt gcaggaaaaa caacaaaaat tccaaaaaat
     2101 agttgatacc tgtggtgtta caaatcttca tggtataccc ggattatttg gtggtttggt
     2161 tgctcttgtt attgttgatg gtattgacgc gggtgcacaa ttgaagggta tattaatcac
     2221 tgcaattatt gctattatag ctggtttatt caccggaaag attgtttcct tgttaggtca
     2281 accaaaactg atttatgacg ataaagctga atttgaaact gaggtttcgg aataggttca
     2341 gcaaccacga tacatttaga aacaattctg ccgtatttct tcttgttaag aaagaaagta
     2401 cggcagtttt gttattataa aattgctaat agaataaaat cttatttgta gccctgttcg
     2461 tgaacacttt taacggcccg acccgatgga tcgaccatat tcttgaacgc ggcatctcat
     2521 tccaatgcaa ttggcgtgct gcacgcaacc gaaggtaccg aaggcacaca cgatgctgct
     2581 gaatcagatg ggaacaattc gtgaaaaatg gtgcggtagt ggtattcttc cttgttcatt
     2641 gttgggttga tcggaaacct ttcggctgcg tttgcaattt cttcatccga aatatttttc
     2701 gaagccaaat ctcttaatgt atcaatccag ctataaccta caccatccga aaactgttct
     2761 ttttgacgcc atgcaacgct ctcgggcaga aaatcttcga acgcttttcg caagatccat
     2821 ttttccattt tcccgtcttt ggccatcttg gcttccggat taagcctcat agcaacatcc
     2881 atgaattctt tgtcgagaaa tggaacgcgg ccttcaattc cccaagctgc caacgattta
     2941 ttggcgcgca ggcagtcgta caaatggagt ttgtcaattt ttcggactgt ttctttgtgg
     3001 aattcttcgg catttggagc tttgtgaaag tagaggtatc caccaaaaat ttcgtcggca
     3061 ccttcgcccg agagtacc
//
LOCUS       DAOPUW010000174         3076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_32695_length_3076_cov_3.943396, whole genome shotgun sequence.
ACCESSION   DAOPUW010000174 DAOPUW010000000
VERSION     DAOPUW010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32695_length_3076_cov_3.943396"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            344..1543
                     /locus_tag="PKJ43_03965"
     CDS             344..1543
                     /locus_tag="PKJ43_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNZ71750.1"
                     /translation="MENKKVVLAFSGGLDTSFCAMYLAKEKGLEVYSAIANTGGFSDE
                     ELKIIEEKAYRLGVKKHVAIDVTEKYYQQSIRYMVFGNVLRNNTYPISVSSERIFQAI
                     AIIEYAKQIGAAYVAHGSTGAGNDQVRFDLTFDVLAPGIKILTPTRDLELTREYEINY
                     LKEHGFEADFKKMEYSINAGLWGTSIGGKETLKSEQTLPEKAYPSQLTETESRDIVIE
                     FDKGEIAAVEGVAYKNPVDAIRALQAIASKYAIGRDMHIGDTIIGIKGRVGFEAAAPM
                     IIIKAHQMLEKHTLTKWQQYWKEQLGNWYGMFLHEALYLEPVMRDIEKFLENSQRNVS
                     GKVFVRLKPYQFQLIGVDSPKDLMKSDFGEYGEMNKGWTADDVKGFTKILGTQLKIFN
                     AVNKDLK"
     gene            1598..2572
                     /gene="argC"
                     /locus_tag="PKJ43_03970"
     CDS             1598..2572
                     /gene="argC"
                     /locus_tag="PKJ43_03970"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNZ71751.1"
                     /translation="MKKIRVGIIGGAGYTAGELLRILLNHPSVDVTYVHSTSNSGKKV
                     TDIHTDLLGDTDLVFSGVYDFSGLDVIFLCMGHGKSVEFMNDNQLPESLKVIDLSTDY
                     RMKRDNNNFVYGLPELNAMEIKSAKYIANPGCFATGIQLALLPLAHAGLLTDEIHVNA
                     ITGSTGAGQSPSKTSHFSWRNNNLSVYKVFEHQHLGEINQSLVQLQPSYKPSLNFIPV
                     RGNHTRGIFVTAYTRFNGSVEDAKNLYRAYYQDHPFVHISSENPDLKQVVNTNKCILY
                     FEKHNEYLVVVSLTDNLIKGASGQAIQNMNILFGLDQKTGLYLKPVAF"
     gene            2679..>3076
                     /locus_tag="PKJ43_03975"
     CDS             2679..>3076
                     /locus_tag="PKJ43_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ71752.1"
                     /translation="MELFNVYPLFDVEPVKGEGTFVFDKNGQRYLDLYGGHAVISIGH
                     SHPHYVANISSQVEKIGFYSNSVQNPLQKELARKLGEACGFPEYSLFLCNSGAEANEN
                     ALKLASFKTGKSNIISFKNSFHGRTSAAVVV"
BASE COUNT          954 a          549 c          655 g          918 t
ORIGIN      
        1 gcaatgcttt acgatccggc ctgggaaaag aaagagcagg ataaaaacga acaaaaaatc
       61 gaagaaaaaa ataatgggaa gatcgtttcg tttaaaaaat ggttgaccaa aggagaatca
      121 actacgggtt ttgcttctcc tctaaaatcg aaatttaaat tatttaaaaa gaactaaaat
      181 ggtgccgcgt tccaatggct ggtaattcgc aattttcata aagccagtat tgtttgcata
      241 gtatgtcgtg taattggttt tttttaatgc ttacaatact tctcattggt atttgggtta
      301 gtaaagttta tttttgcggc accatttttt tcatcaaaat atcatggaaa acaagaaagt
      361 agttttggcg ttcagcgggg ggttagatac ttccttctgt gctatgtatt tggcaaaaga
      421 aaaaggactt gaagtatatt cagccattgc caataccgga gggttctccg acgaagaact
      481 caaaatcatt gaagaaaaag cctacaggct tggagtgaaa aaacatgtgg ccatcgatgt
      541 tacagaaaag tattatcagc aaagcatccg ctacatggtt tttgggaatg tgttacgaaa
      601 caatacctat ccaatttcgg ttagttccga acgaattttt caagctattg caattattga
      661 atatgccaag caaattggag cagcatacgt agctcacggg agtacaggtg ccggaaacga
      721 ccaggtacgc tttgacctta cttttgatgt gcttgcccct ggaataaaaa tacttacgcc
      781 tacacgtgat cttgagctta cccgtgaata cgagataaac tatctgaaag aacatggttt
      841 tgaggccgac tttaaaaaga tggaatattc cattaatgca ggtctttggg gtaccagcat
      901 tggcggaaaa gaaaccctga aatcggaaca aacactgccc gaaaaggctt atcccagtca
      961 gttaactgaa accgaaagtc gtgatattgt tatcgagttc gataaaggtg aaattgcagc
     1021 cgttgaaggt gtcgcttata aaaatcctgt cgacgctatt cgcgccttgc aagctatcgc
     1081 ttcgaaatat gccattggcc gcgatatgca tattggcgat acaattattg ggattaaagg
     1141 tcgtgtaggt tttgaagctg ctgctccaat gattattata aaagcgcatc aaatgcttga
     1201 aaaacacacg cttactaagt ggcaacaata ttggaaagaa cagcttggaa attggtatgg
     1261 catgttcctt cacgaagcat tatatcttga acccgtaatg cgcgacatag aaaagttcct
     1321 cgaaaattca cagagaaatg tatcaggaaa agtatttgtt cggttgaaac catatcaatt
     1381 ccagttaatt ggcgttgact ctcctaaaga ccttatgaaa tctgattttg gcgaatacgg
     1441 cgaaatgaac aaaggctgga ctgccgacga tgtgaaaggg ttcacaaaaa tacttggaac
     1501 ccagttgaaa atattcaacg ctgtaaacaa ggatttaaaa taatttgaca agttgcgtga
     1561 attattggtt ttgcaactct ctatttattg attgattatg aaaaaaatac gcgttgggat
     1621 tattggtggg gcagggtata ctgcaggcga actattgcgc atattattga accatccgtc
     1681 tgttgatgtt acctatgtgc acagtaccag taactctgga aaaaaagtca cagacatcca
     1741 caccgacctt cttggcgata ccgacttggt tttttctgga gtatatgatt tttcagggct
     1801 cgatgttatc tttttgtgca tggggcatgg gaaatcggtt gaattcatga atgataatca
     1861 attgcctgaa agtcttaagg ttattgattt gagcaccgat tatcgcatga aacgtgacaa
     1921 taataatttc gtgtatggtt tgcccgaatt gaatgccatg gagataaaga gtgcaaagta
     1981 tattgccaac ccaggttgct ttgctacggg aattcaattg gcacttttgc cattggctca
     2041 tgccgggttg ctgactgacg aaattcatgt caatgccatt accggttcaa ccggggctgg
     2101 tcaaagtcca tcgaaaactt cgcatttcag ttggcgtaat aataatcttt cagtatataa
     2161 agtttttgaa catcagcatc ttggtgagat aaatcaaagt ctggtacagt tacagccaag
     2221 ttacaaacca tcgctaaact ttattccagt aaggggaaat catactcgtg gaatatttgt
     2281 gacagcttac acccgtttta acgggagtgt agaagacgca aaaaatctct atcgggccta
     2341 ttatcaggat catccgtttg tacatatttc cagcgaaaac cctgatttga agcaggtagt
     2401 taataccaat aaatgcattt tgtacttcga aaagcataac gaatatcttg tagttgttag
     2461 cctcaccgat aacctcatca aaggagcttc ggggcaggct attcaaaaca tgaacatatt
     2521 gtttggctta gatcaaaaaa ctggattata tttgaagcca gtagcttttt aagctttttg
     2581 tcctggacaa aacgtggttc gggaattttt attcattgat tgttagtgtt ttattaatta
     2641 atgagattac tgcaattaat cactttaata actaagaaat ggaattattc aatgtttatc
     2701 ccctttttga tgtagaaccg gttaaaggag aagggacttt tgttttcgat aagaacgggc
     2761 agcgttacct cgatttgtat ggtggccacg cagttatttc tatcggtcat tctcacccgc
     2821 attatgtggc aaacatttcg tcgcaagtag aaaaaattgg tttttattcc aattcagtac
     2881 aaaacccctt gcagaaagag ctcgcccgaa aacttggtga agcatgtggt tttcctgaat
     2941 attcactttt tttgtgtaac agtggcgcag aggctaatga aaatgcctta aaactggctt
     3001 cattcaaaac cgggaaatcg aatataattt cattcaaaaa ttcgtttcat ggtcgtactt
     3061 cggctgcagt tgtggt
//
LOCUS       DAOPUW010000175         3073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_32736_length_3073_cov_3.377071, whole genome shotgun sequence.
ACCESSION   DAOPUW010000175 DAOPUW010000000
VERSION     DAOPUW010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32736_length_3073_cov_3.377071"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1264
                     /locus_tag="PKJ43_03980"
     CDS             167..1264
                     /locus_tag="PKJ43_03980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNZ71753.1"
                     /translation="MNFLQALQYCIDNRLIFAAYRLPHSDAIKLVVQKNPTPKAETIN
                     SELFEKQGFIFSPFVKSEKNPSYLIQPDLVYSSTNYINFDELFQLQAIAQEENSYENG
                     VIGKKEFKAQVESIIEAIHQNKFKKAVLSRTKVLERNYRCKLKEIFQLLSTSYVNAFV
                     YMVNVGGQFWIGATPEPLLCAHKNELEAVSLAGTKPYNDENLNITNWSHKDQIEQELV
                     TQYIEETFESRGIQFTQKSGPYTKKAGNLVHLRTDFTYNLADVGKHLGELVSELHPTP
                     AVCGLPKQAAMDFIQNIEKHQRGYYSGFLGPVNIDNKLQLFVNLRCMQVMDERLILYI
                     GAGITAQSDAEEEWNETEIKADTLLSVIHKV"
     gene            complement(1315..2757)
                     /locus_tag="PKJ43_03985"
     CDS             complement(1315..2757)
                     /locus_tag="PKJ43_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ71754.1"
                     /translation="MESKIIGRGKELKIIDKLLLSEKAEFLAVYGRRRVGKTFIVREY
                     LKAHIHFAFSGSYEEKVSVQIDNFFHEYIRRTGGKKETVVPPNWSTAFQYLADILYNM
                     KDSPTKVVVFIDELPWLDTPKSGFVQALEYFWNQHVSTMNHVLLVVCGSAASWMQQKL
                     FKNKGGLYNRITKRIKLEPFTLFETELYCKSKKLKLTRYQIIQLYMVMGGVPFYWNEL
                     SQGKSAEQLIDEICFESTGLLATEYDQLYYSLFKNAENHIAVIEALASHPYGLVREEL
                     LKKSGLPDGGTFTRTIEDLYESGFIAKHRPFEKKVKETQFRLVDMYSLFYLNFIKDNV
                     KNRANTWQKIANQPRFVSWSGYAFENICLQHSNQILIKLGISGTFTEISSWRHKGNDE
                     LPGAQIDMLIDRKDGMINLCEAKFSNKEFIIDKDYIATLRQKRAVFSHVTGTKKSVVT
                     TLITTYPAMQNNYYLEEIHSEVTMDDLFVE"
BASE COUNT          904 a          704 c          581 g          884 t
ORIGIN      
        1 gaaaccacat ttcgtctatt cgcgagggtt gggtcattgc ccgcgcaacc atcattcacc
       61 aagggcgcac cagccatgtt tgggatgttg aaattcgcga cacagacggt aaacttattt
      121 cggtggcccg tgttactaat gccattgtgg aaaagaaaac agaaaaatga atttcttaca
      181 ggcacttcaa tattgtatcg acaatcgctt gatttttgcg gcatacagat taccgcactc
      241 cgatgccatt aaacttgttg tacaaaagaa tccaaccccg aaagccgaaa ccatcaattc
      301 tgaattattt gaaaaacaag gttttatatt ttcaccattt gtgaagtctg aaaaaaatcc
      361 cagctatttg attcagcccg atttggtgta cagcagtacc aactacatca attttgatga
      421 gctatttcag ttgcaagcca ttgctcaaga agaaaattca tacgaaaacg gcgtcatcgg
      481 gaaaaaagaa tttaaagctc aggttgaaag cattattgaa gccattcatc agaataaatt
      541 caaaaaagcc gtactctcgc gcaccaaagt tttagaacgc aattaccggt gtaaactcaa
      601 ggaaatcttt cagttactca gcacaagcta cgtcaatgct tttgtgtaca tggtgaacgt
      661 aggcggccaa ttttggatag gtgcaacccc cgagcctttg ctttgtgctc acaaaaacga
      721 attggaagca gtgtcgctgg caggcacaaa accttataac gacgagaacc tgaacattac
      781 caattggagc cacaaggacc agattgaaca agaactcgtt actcagtata ttgaagaaac
      841 ttttgagagc cggggcattc aattcactca aaagagcggt ccttacacga aaaaggcggg
      901 caatctggtt cacctccgca ccgatttcac ttacaacctg gccgatgttg gcaagcactt
      961 gggcgaactt gtttccgaac tacaccctac cccagccgtt tgtggacttc cgaagcaagc
     1021 tgccatggac ttcattcaaa atattgaaaa acaccaacga ggatactact ctgggttttt
     1081 gggtccggta aacatcgaca acaaacttca gctatttgta aaccttcgtt gcatgcaggt
     1141 tatggacgaa cggcttatcc tttacattgg tgccgggatc actgcccaat cggacgccga
     1201 agaagaatgg aacgaaaccg aaataaaagc cgacacgctg ctgtcggtaa ttcacaaggt
     1261 ttaattctgt aaattcatta cttttcccct ccttaggagg ggttagggga ggctttattc
     1321 tacaaacaaa tcgtccatcg ttacttccga atggatttct tcgagataat aattgttctg
     1381 catggccgga taggtagtta tcagtgtagt taccaccgac ttctttgtac cggtaacgtg
     1441 cgaaaaaaca gcccgtttct gccttaacgt agcgatataa tctttatcaa taatgaattc
     1501 cttgtttgag aatttggcct cgcataggtt aatcatacca tctttccggt caataagcat
     1561 gtcgatttgc gcgcccggca attcgtcgtt gcccttgtgc ctccacgacg aaatttcagt
     1621 aaatgtgcca ctaataccca acttaatcaa tatctggttg ctgtgttgaa ggcaaatatt
     1681 ttcaaaggca tatccgctcc acgaaacaaa ccggggttga ttggctattt tttgccaagt
     1741 gttcgccctg tttttcacat tgtctttaat aaaattcagg tagaaaagcg aatacatatc
     1801 aaccaagcga aattgcgttt ccttaacctt tttctcgaac ggacggtgtt tggctataaa
     1861 accgctttcg tacaaatctt cgatggtgcg tgtaaaagtt ccgccatcgg gcaaccctga
     1921 tttcttgaga agttcctctc gaacaagccc atacggatga ctggccaaag cttcgataac
     1981 ggcaatatgg ttttcggcat tcttaaaaag cgaataataa agttgatcgt attcggtggc
     2041 aagcaagccc gttgattcga aacaaatttc atcgatcaat tgctccgcac tttttccctg
     2101 gcttagctcg ttccaataaa acggtacacc gcccatcacc atataaagtt gaataatctg
     2161 ataacgggtc agtttcagct tcttgctttt gcaatacagt tcagtttcga acaaggtaaa
     2221 cggctccaat tttatccgtt tggtaatgcg attgtaaagt ccgcctttgt tcttgaaaag
     2281 tttttgctgc atccacgaag cagccgaacc gcaaaccacc aaaagtacat ggttcatggt
     2341 tgaaacatgt tggttccaaa agtattcaag tgcctgcaca aaacccgatt ttggcgtatc
     2401 caaccacggc aactcgtcga taaacaccac cacttttgta gggctgtcct tcatattgta
     2461 caatatatca gctaaatact gaaaagcggt actccagttt ggcggaacaa ccgtttcctt
     2521 tttcccgccg gtccttctga tgtattcatg aaaaaaatta tcgatctgaa ccgaaacttt
     2581 ttcttcgtac gagcctgaga atgcgaaatg tatatgagct ttcaaatatt cgcgcactat
     2641 aaatgtcttc cccactcgcc tccgtccgta cacagccaga aactcggcct tctccgacaa
     2701 taaaagcttg tcgattattt tcagctcttt gccacgacca ataatcttac tttccatgat
     2761 tattctgatt ttcaatactc aaagatataa aacatttttc atacttggag aaatcttcat
     2821 ttttaaatcg catttctcca agtatgtgct ctttatgtgg aattatcttc gttataaaaa
     2881 gattttccac caaatttaaa aactcatatt tggagaaatc tacatttttt caccacattt
     2941 ctccaagtat gaatatcgaa aaacaaaaac acctacaaaa tggtttcctg ccattattgc
     3001 caatcgaaga catttcgtta ctttgcatac ttatgattca tcaccttcag catattgtcg
     3061 atttggttgc gat
//
LOCUS       DAOPUW010000176         3059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_32952_length_3059_cov_2.896471, whole genome shotgun sequence.
ACCESSION   DAOPUW010000176 DAOPUW010000000
VERSION     DAOPUW010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32952_length_3059_cov_2.896471"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>875
                     /locus_tag="PKJ43_03990"
     CDS             <1..>875
                     /locus_tag="PKJ43_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HNZ71755.1"
                     /translation="TDEYAYESIERLQELQKTQDNLELRLSTVVKDISLLTPWGDFSW
                     DIIRKLRHQGWQLTFYSCPTRKIDSTWNEKFFFEIISQDSSTTNFITITKAGELIDLD
                     LESIHLPEKSLSSLLAEEVSIKEQLRQIADSYSNLAIDSINTLKQLQISALADLDFRK
                     VVLNTQKQADDRLMLLEGWAPAENHEGISNFLDEKGVVWFSHKAEKEDPAPIKLTNNK
                     FARLFHPITELYEMPAYGEIDLTPFFAPFFVMFFGLCLGDAGYGVLLLLIGLIARKKV
                     KEDMKPIMTLVSILG"
     assembly_gap    876..885
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <886..1591
                     /locus_tag="PKJ43_03995"
     CDS             <886..1591
                     /locus_tag="PKJ43_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HNZ71756.1"
                     /translation="IIFGFLSGTLFGIELAKVEWPWIQRFKSIMLDPNDLFSFSLIIG
                     GVQILFGMTIKAIGQTIRFGFKNALSTWGWLVAIIGCGGVFGMQKLGILSQQSATVGY
                     YITGGIAFLGIFILNDIKRNPLINIGAGLWDTYNMITGLLGDVLSYVRLFALGISGAV
                     LGLVFNDLAIKMSPDIPVVGFLVSAVILLFGHGMNIFMAGLGSFVHPLRLTFVEFYKN
                     AGFEGGGKKYNPFRKG"
     gene            1642..2091
                     /locus_tag="PKJ43_04000"
     CDS             1642..2091
                     /locus_tag="PKJ43_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HNZ71757.1"
                     /translation="MQPIVLAYIGVGLMLGLSGIGSAFGVTITGNATVGALKKNPDKF
                     GNYMVLSALPGTQGLYGFLGYFLLKGALLADMGWLAASGVFGAGLGLGLVCLFSAIRQ
                     GQVCANGIAGIGTGYDLFGKTLILAVFPELYAIIAVAATFLISGSLV"
     gene            2209..2583
                     /locus_tag="PKJ43_04005"
     CDS             2209..2583
                     /locus_tag="PKJ43_04005"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNZ71758.1"
                     /translation="MKNLKKYYKLGASPADIYNALTNQIMIEIWTGDKADFKAEPGYE
                     FSLWDGAITGRNVAFEMDRKIKQIWFFDEVESEVIITLHPDKKGTSVELVHNNIPDEA
                     FENISEGWDLDYFSSLAELFND"
     gene            complement(2580..3005)
                     /locus_tag="PKJ43_04010"
     CDS             complement(2580..3005)
                     /locus_tag="PKJ43_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71759.1"
                     /translation="MKKLRRYILPMALIITTGLLAMSYVFRSPENSVAKEQAVFQIGA
                     NDLFSSFETDENAANEKFIGKVIEVSGEVVSTEQTANGQLEVLLMVENPMGGVRCVFE
                     TAQQKVIGKLKTGQTASIKGKCSGMLMEVVLDNCALTGN"
BASE COUNT          837 a          541 c          654 g         1017 t
ORIGIN      
        1 aactgatgag tatgcatacg agagcattga acgtttacaa gaattacaga aaacgcaaga
       61 taatttggag ttgcgtttgt ctaccgtagt aaaggatatt tcactactta ccccttgggg
      121 cgatttttcg tgggatatta ttcgtaagct caggcatcag gggtggcaat tgacctttta
      181 ctcatgtcca acacgtaaaa tagattcaac ttggaatgaa aaattctttt ttgagataat
      241 cagtcaggat tcttccacta cgaattttat taccattaca aaagcaggag aattaattga
      301 ccttgattta gaaagtattc atttaccaga aaaaagtttg tcttcacttc tggcagaaga
      361 agtaagcatt aaagaacaac tgcgacaaat tgccgattcc tatagtaatc ttgccattga
      421 ttcaattaat acgctcaaac agttacaaat cagtgcattg gctgatctag attttcggaa
      481 agttgttctt aatacacaaa aacaagctga cgatcgttta atgcttctcg aaggctgggc
      541 cccagcagaa aaccatgaag gaattagtaa ttttcttgat gaaaagggag ttgtatggtt
      601 ttctcataag gcagaaaaag aagacccggc acccatcaag ttaaccaata ataaattcgc
      661 ccggcttttc catcctatta ccgaactata cgagatgcct gcatatgggg aaatagacct
      721 tactccgttt tttgctccat tttttgtaat gttttttggc ctttgccttg gcgatgccgg
      781 atatggagtg ttgttgcttt tgattggttt aattgcccgt aagaaagtaa aagaagacat
      841 gaagcctatt atgactttag tttctatttt gggtgnnnnn nnnnnaatta tcttcgggtt
      901 tcttagtgga acattatttg gcattgagtt ggcaaaggtt gaatggccat ggattcagcg
      961 ttttaaatca attatgctcg atcccaatga cttgttcagt ttctcgctaa ttattggtgg
     1021 agttcaaata ttgtttggaa tgactattaa agccattggt caaacaattc ggtttggatt
     1081 taaaaatgcg ttatctacat gggggtggtt agtggctata atcggttgtg gtggtgtttt
     1141 tggaatgcaa aagttaggaa ttctttccca acaatctgcc actgtaggct attatataac
     1201 tggtggtata gcctttctgg gcattttcat actgaacgac ataaagcgca atccattaat
     1261 aaacattgga gctggtttat gggatactta taatatgatt accgggctgt taggtgacgt
     1321 tttatcatac gtccgtcttt tcgcattggg aatctctggt gcggtattgg gcttggtgtt
     1381 taatgattta gcaataaaga tgagccccga tattcctgtt gtcggatttt tagtttcagc
     1441 agttatcctg ttgtttggcc acggaatgaa catttttatg gcaggcctag gatcgtttgt
     1501 gcatccgctc cgtttaacgt ttgtagaatt ttacaagaat gccggatttg aaggcggagg
     1561 aaagaaatat aaccctttca gaaagggata attgtgttat gtaaattagt aaaaataaat
     1621 aataattaaa cgattaaaat tatgcaacca attgttttag cttacatcgg cgtaggattg
     1681 atgctcgggc tctccggaat aggtagtgct tttggggtaa caatcactgg aaatgccacg
     1741 gttggggctt taaagaaaaa tccggacaaa ttcggtaatt acatggtcct ttctgcattg
     1801 cccggaacac aaggcctcta cggttttctt ggttactttt tgttaaaagg agcgttattg
     1861 gccgacatgg gatggctggc tgcgtctggt gtttttggcg cagggctggg cttaggctta
     1921 gtgtgtttgt tttcggccat tcgacagggg caggtatgtg ctaatggtat tgcaggaatt
     1981 ggcactgggt acgacttgtt tgggaaaact cttattcttg ccgtttttcc tgaactttat
     2041 gcaattattg ctgttgctgc aactttcctt atttcgggtt cgcttgtata agaaagctat
     2101 ttttaacgaa caacccggga aatgtcgata acctgatttt cccgggttgt ttgtttccat
     2161 tcaatcttat cgtacttttg cacctttcat cttttattct atgatactat gaaaaatctt
     2221 aaaaaatatt acaaactggg cgcttcgcct gccgatatct ataatgctct tactaatcag
     2281 attatgattg agatatggac tggtgacaaa gccgatttta aagcagaacc gggctacgaa
     2341 ttttctttgt gggacggggc aattaccggt cggaatgtgg ctttcgaaat ggaccggaaa
     2401 ataaaacaga tatggttttt cgatgaggta gagtcggaag ttattataac ccttcatcct
     2461 gacaaaaaag gaacgagcgt tgaacttgtc cataacaata ttcccgatga agcttttgaa
     2521 aacatctcgg aagggtggga ccttgattat ttcagttcgt tggccgaact ctttaacgat
     2581 taattcccgg ttaaagcgca attgtcgagg acaacttcca taagcatgcc cgaacatttg
     2641 ccttttatac ttgcggtttg tccggttttt agcttcccga taactttttg ctgagcggtt
     2701 tcaaatacac aacgaacacc gcccatgggg ttttctacca ttagtagtac ttctaattgg
     2761 ccatttgcgg tttgttctgt tgaaacaact tcaccactta cttctattac tttgccaata
     2821 aatttttcat tggctgcatt ttcatcggtt tcaaacgatg aaaataagtc gttagctcca
     2881 atttgaaaaa ctgcttgttc ttttgccacc gaattttccg ggctgcggaa tacataactc
     2941 attgctaaca aacctgtagt aatgattaat gccattggca ggatgtatct tcttaatttt
     3001 ttcattggga ctgatttacc tattatattt attttgaata aggtacatat tcaacatct
//
LOCUS       DAOPUW010000177         3055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_33017_length_3055_cov_3.333000, whole genome shotgun sequence.
ACCESSION   DAOPUW010000177 DAOPUW010000000
VERSION     DAOPUW010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33017_length_3055_cov_3.333000"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..1135)
                     /locus_tag="PKJ43_04015"
     CDS             complement(194..1135)
                     /locus_tag="PKJ43_04015"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNZ71760.1"
                     /translation="MTLLQLSYVLELSRYNSFSLTAKRLNISQPALSLQIRKLEEELG
                     IHLFKRSPTAVTLTAEGELFVDKARELLALAEKLKDLPFELEEKPEGTLRIGVIPTLA
                     PYWFPMFMNSLSKKYPKIMLTVSEMKTDDIIAELKNGQLDAGFLSTPVHAPGMEFRTL
                     FYEKFFLYVSDKNELFGKETLNLKNIDLKELWYLQEGNCFQNQVNSICKYAHEPNELQ
                     NVVYLSNSIESLCRMVETSGGMTFIPELATLAVSSDEEEMIKEIEGEAPLREISMITT
                     RISKSDRLLELFLNVALSVIPQRMKTIENGKVLSTGI"
     gene            1239..1688
                     /locus_tag="PKJ43_04020"
     CDS             1239..1688
                     /locus_tag="PKJ43_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HNZ71761.1"
                     /translation="MTTKNLNKVSSQLNQVLADLQVVYQNLRTMHWLVKGAQFHQLHK
                     LYEEMYNETAETIDEVAERVLMLGQVPLHTYADYLKVAKVEVVTQVPKGMESLKTALE
                     NQQYLLNAYRELISLAGENSDEGTVVLASELIASTEKRVWMLGATLS"
     gene            1746..2459
                     /locus_tag="PKJ43_04025"
     CDS             1746..2459
                     /locus_tag="PKJ43_04025"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNZ71762.1"
                     /translation="MENNEVTVVSLPRIGEKAPSFEAVTTQGNIKFPGDYAGSWVILF
                     SHPADFTPVCTSEFMTFASMEEQFASVNCKLVGLSVDGLYSHIAWLRTIKEKIEYKGM
                     KDVEVKFPLIEDITMEVAKKYGMIQPGESNTKAVRAVFIIDPKGVVRTIIYYPLSLGR
                     NFDELYRAVVALQTADEFSVATPADWRPGDDVIVPTAGSCGVAKQRMESKEEMTCYDW
                     FFCTKKLSKEEVLSKIVKK"
     gene            complement(2507..>3055)
                     /locus_tag="PKJ43_04030"
     CDS             complement(2507..>3055)
                     /locus_tag="PKJ43_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71763.1"
                     /translation="QMTTMTKSELEEMVASAYSKADYQYLFQRATLEPAFFKLVWQVA
                     SEKPDKQSWRFLWILDHATEKSNTNLLPICSDLYKMVLATKNNSVIRQSMKLILRCPI
                     NEEHAGALLDRCISWMNNPKETISSQCMGLEFFFRCCELYPEMSSELLSHIEENLERH
                     PSAGFKSRLKYVRKKLIDEANK"
BASE COUNT          889 a          609 c          661 g          896 t
ORIGIN      
        1 cacagaattg atctgtatgt tacaccgctt aattttaaaa atgtgaaagg acgtttggga
       61 tggtcttttc atatagccaa tggcgacctc gacaaccagc aacagttttc actgctctat
      121 acttttggca acgatttgtg cagaaagcaa taaatcagca tttttttaat tgagctgata
      181 aaacaaattc aacttatatt cctgtggaaa gaactttccc gttttcaatt gttttcatgc
      241 gttgaggaat aaccgataag gcaacattga ggaagagctc aagcaggcgg tccgatttgc
      301 tgatccgtgt agtaatcatg ctaatttccc ggagcggtgc ttcgccctca atttctttga
      361 tcatttcttc ctcatcgctg ctaaccgcaa gggtagctaa ttccggaata aaagtcattc
      421 ctccgcttgt ctctaccatt cggcacagcg attctattga atttgataga taaactacat
      481 tttgcagttc gttgggttcg tgggcatact tgcaaataga attgacctgg ttctgaaagc
      541 aattcccttc ctggagatac caaagctctt tcaggtcgat gttttttaag ttgagggtct
      601 cttttccgaa aagctcattt ttatcagaca catataaaaa aaacttttca taaaacaagg
      661 ttctgaactc cattccggga gcatgtaccg gggttgatag aaatcctgca tccaattgcc
      721 cgttttttag ttcagcaatg atatcgtcgg tcttcatttc ggaaaccgta agcataattt
      781 tgggatattt tttcgaaagg ctgttcataa acatagggaa ccaataaggt gccagagttg
      841 gaatcacccc aatccgcaat gtcccttcgg gtttttcttc cagctcgaag ggcagatctt
      901 ttagtttctc tgccaatgcc agcagttcac gggccttgtc aacaaaaagt tcaccttctg
      961 ccgttagggt caccgcagtg ggtgagcgtt tgaaaaggtg aatacccaac tcttcttcaa
     1021 gctttctgat ttgcaaactt agcgcaggtt gcgaaatatt taaccgtttt gcagtaagcg
     1081 aaaacgaatt gtaccggctc aattccagta cgtagcttaa ttgaagtaat gtcatgatat
     1141 aatcttttgc ttatatgttt atttgtatcg ataaatatat ccaataatat ctttgtcata
     1201 tcaaaatgaa taagaacaat aaccaaaaaa ttaatatcat gacaacaaaa aatttgaata
     1261 aagtaagcag ccagctaaat caggtattgg ccgatttaca ggtagtatat cagaatctcc
     1321 gtaccatgca ttggttggtt aaaggggcgc aatttcatca gcttcacaag ttgtatgaag
     1381 aaatgtataa cgaaaccgcc gaaacaatcg acgaagtggc cgaacgggtg ttaatgctag
     1441 gccaggttcc actgcatact tacgccgatt atcttaaggt tgctaaggtt gaagtagtta
     1501 cacaagttcc gaagggcatg gagagtttaa aaactgcact cgaaaaccag caatacttgc
     1561 taaatgccta tcgcgaactt atttcacttg ccggcgagaa cagtgatgaa ggaacagtag
     1621 tacttgctag tgagctaatt gcatcaaccg aaaaaagagt ttggatgcta ggagcaacat
     1681 tatcataaat atcaatgtta tagtattaaa cagaattatt aactaaccat ataaatatag
     1741 aagaaatgga aaataatgaa gtaacagtag tttcgttgcc acggattggc gaaaaagctc
     1801 catcgtttga agcagtaacg acccagggaa acatcaaatt tcctggcgat tatgcaggaa
     1861 gctgggttat cctttttagc caccccgccg atttcacccc ggtttgtact tccgagttta
     1921 tgactttcgc ctcgatggaa gaacaatttg ccagtgttaa ttgtaagctg gtaggccttt
     1981 cggtcgacgg actttacagc cacattgcat ggctccgtac aatcaaagaa aaaatcgagt
     2041 acaaggggat gaaggacgtg gaagtgaaat tcccgttaat tgaagatatt accatggaag
     2101 tagctaaaaa atatggtatg attcagccgg gagaaagcaa cactaaagcc gttcgtgcgg
     2161 tgtttattat cgatccgaaa ggggttgtac gaacaataat ttattacccg ctcagtcttg
     2221 gccgtaattt cgacgaactt taccgtgctg tggtggcctt gcaaactgcc gacgaattta
     2281 gtgttgccac tccggccgac tggagaccgg gcgatgacgt aattgttcca accgcaggtt
     2341 catgtggggt agcaaaacaa cgcatggaaa gcaaagaaga aatgacttgt tacgattggt
     2401 tcttctgtac caaaaagttg agcaaagaag aagtgctttc gaaaattgtg aagaagtaat
     2461 atactataat ttaacttgtt agagccctgc aattgtgggg ctctttttat ttgtttgcct
     2521 cgtcaatcag tttttttctt acatacttga gcctcgactt gaacccggca gaggggtgtc
     2581 tttcgaggtt ttcttcgatg tgcgaaagca attccgaact catttcggga tacaattcgc
     2641 agcagcggaa aaaaaactca agtcccatac attggctcga aatggtttct ttcggattgt
     2701 tcatccatga aatgcaacgg tcaagaagtg ctccggcatg ttcttcatta atcgggcacc
     2761 tgaggatcag tttcatgctt tgccggataa cactgttgtt ttttgtggca agaaccattt
     2821 tgtacaggtc gctgcaaatg ggcaataggt tggtgttgct cttttctgta gcatggtcga
     2881 gtatccacaa aaaacgccac gattgtttat ctggtttttc gctggcaact tgccaaacaa
     2941 gcttaaaaaa tgcaggctca agggttgccc gctggaataa atactggtag tcggctttcg
     3001 aataggccga agctaccatt tcttcaagtt cgcttttggt catggttgtc atttg
//
LOCUS       DAOPUW010000178         3054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_33030_length_3054_cov_4.921641, whole genome shotgun sequence.
ACCESSION   DAOPUW010000178 DAOPUW010000000
VERSION     DAOPUW010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33030_length_3054_cov_4.921641"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..518)
                     /locus_tag="PKJ43_04035"
     CDS             complement(135..518)
                     /locus_tag="PKJ43_04035"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNZ71764.1"
                     /translation="MDAAVEIVRLTQVDFYNGYKSARKVIDTGSGSLSVKGIVADAQT
                     GLPLKGVTVSFALEGGMMKTVAATTTDPELVKKTAEKGGFNVKNLASGTYQVTLKKVG
                     YADQVITVNVNDGEMSLIDVRLDKQ"
     gene            complement(777..1871)
                     /locus_tag="PKJ43_04040"
     CDS             complement(777..1871)
                     /locus_tag="PKJ43_04040"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNZ71765.1"
                     /translation="MKQDAKIVVDMFESQVPINKRLSWAGLSASTFYYKSRTGKPGRK
                     ASTTTMKTDGSVVTNHQVVEEIKSILDIEFLCYGYIPITDELFDRGYLINHKKVYRIM
                     RENKLLCGKVISSHAGKRQFVRFRKIEANYPLQYLCMDIKYIYIEGQKKYAYLLSLID
                     VYSRRIVGYVFKNSIKQHDVLWLLKHAVGENQKVSITIRNDNGSQFIAKSVREYLKEI
                     QVTHEFTHIATPEENAYIEAFHSILERELTSRYEFDSFYHAEMKIAQYMFTYNNIRKH
                     RAIGLRTPQRAWDEYFGSLSTDRQPTAVEPEGLSRLADSKNKITNQSAMHFNLYKPGG
                     SAIFDYLLANENIRNYICKNSQTIFQKHSN"
     gene            complement(1868..2164)
                     /locus_tag="PKJ43_04045"
     CDS             complement(1868..2164)
                     /locus_tag="PKJ43_04045"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNZ71766.1"
                     /translation="MTKERRQWSEQQKLQIIQEVEVNGLLPVLRKYDLSQSLFHKWKR
                     KFNAQGVAGLAPQYPKIDPEKRRMEQEIKRLREMVGELSMELRFKDELLKKSQY"
     gene            complement(2774..>3054)
                     /locus_tag="PKJ43_04050"
     CDS             complement(2774..>3054)
                     /locus_tag="PKJ43_04050"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNZ71767.1"
                     /translation="ELFFIDERSVFIRTFQGKTYDVDYSLDQLQQLVDPDQFFRINRN
                     YLVNIDAVTKLVSYSSSRFKLELQPEFRADDLIVAREKAGEFKKWMDR"
BASE COUNT          805 a          671 c          579 g          999 t
ORIGIN      
        1 ataacttacc cgtcttcatt tttctttggt gttaatctcg taagcttttt atgttgatga
       61 aactgaacac aaataggttg cgttcagttt cacatattag aaaatacaga tgaccctaca
      121 atgtgtattt gaaactattg tttgtccaat ctaacatcga tcaggctcat ttcgccatcg
      181 ttaacattca ccgttataac ttggtcggca taaccaacct tcttcaatgt aacttgatag
      241 gtgccagaag ccaggttctt aacattaaat cccccttttt cagcggtctt ttttaccaac
      301 tcggggtcag tggtagttgc tgccacagtt ttcatcattc caccttctaa cgcaaacgat
      361 acggttactc ctttcagtgg taaacctgtt tgagcatcgg caacaatgcc ttttaccgac
      421 aatgaaccag aaccagtatc aataactttt cgtgccgatt tgtacccgtt gtaaaaatca
      481 acttgggtca aacgtacaat ttcaactgct gcatccatgt tttctaaagc ggtatcggca
      541 gttttaaaca aggttgcaag ttgcttggtc agttgtgttc cctcgcttct ttctgcacca
      601 ggtttgccaa tcgaatcatt ataagccgta attgcaccag caagggtcgc ttgtgtttca
      661 tcggtaatac cataaggtgc gagagagcca acaagtggtt gggctaggtc ataaacaatc
      721 tgtccatagt ttttaactgc cgtatcggct acctgtctta atttctataa cccctattaa
      781 ttggaatgtt tttgaaaaat agtttgactg tttttacaaa tatagtttct aatattctca
      841 ttcgccaaca gatagtcaaa gatagccgaa cctccgggtt tgtaaaggtt aaaatgcatg
      901 gccgattgat tggttatttt gttttttgaa tcggccaacc ttgacaaacc ctccggttct
      961 accgctgttg gttgcctatc ggttgacaat gaaccgaagt actcatccca tgccctttgc
     1021 ggggttctta gcccgattgc acggtgtttc ctgatgttgt tgtaggtaaa catgtactgt
     1081 gcaattttca tctctgcatg gtagaacgaa tcaaattcat aacgtgatgt caattcacgt
     1141 tcaaggatgc tgtggaacgc ttcaatgtat gcgttttctt cgggtgtcgc aatgtgggtg
     1201 aactcatggg ttacctgtat ttctttgagg tattcgcgta ccgattttgc aataaactgg
     1261 ctgccgttat cattgcggat tgtgatcgag accttctggt tttcaccaac ggcgtgtttt
     1321 aagagccaga gcacatcatg ttgtttgatg ctgtttttga acacgtaacc tacgatacgg
     1381 cgcgagtaaa catcgatcaa agacaaaaga taggcatact ttttttggcc ttcgatatag
     1441 atgtatttga tgtccatgca caggtattgc aaagggtagt tagcctctat tttacggaac
     1501 cgcacaaact gccgtttccc ggcatggctg ctgatcactt taccacataa caatttattc
     1561 tcgcgcataa tacggtagac tttcttgtgg ttgatcagat agccccggtc aaagagctca
     1621 tcggttatag ggatgtaacc atagcataag aactcaatgt ccagaatgga ttttatctct
     1681 tcaaccacct ggtggtttgt gaccacactg ccatctgttt tcatggttgt tgtacttgcc
     1741 tttcttccag gtttccctgt ccttgatttg taataaaaag tgctcgcaga caacccagcc
     1801 catgaaagcc gcttatttat tggaacttga gactcgaaca tatcgaccac tatttttgca
     1861 tcctgtttca atattggctt ttttttaaaa gctcatcttt aaacctgagc tccatactca
     1921 attctccaac catctctcgc agccgcttga tctcttgttc cattctccgt ttttccggat
     1981 caatcttggg gtattggggg gctaaccctg ctacgccttg agcattgaac ttgcgtttcc
     2041 atttgtgaaa caacgattgc gataaatcat acttgcgaag tactggtaat aacccgttta
     2101 cttcaacttc ctgaataatc tggagctttt gttgctcgct ccattgacgc ctttcttttg
     2161 tcataacttt acaaagataa gtgtcaaact gtttctccct ttttattcca cttaattaag
     2221 gggctagtac aaaataagtc catgcaataa tcattaagac agtaaaactc tctgatttga
     2281 acttaatcaa tgggtcatta aaaatcttaa ctgctgctaa agcgctttct tttgactttt
     2341 caagcagttc attttgtatc gaccagattc ttctttttct catatcattt gattcgtcga
     2401 aagcgtcttt ttaaatggca gccaactcgt taatatgcct aaatatgtct tcttttccaa
     2461 tattactatg ttcaacaaag cacattgctt ttcctggttt ggtcacattc cgcaagagaa
     2521 cctgagcata ttggaattcc agtactagtc tcttactgaa cactattaaa cgaaaagagg
     2581 catcaaataa acaggtaatt ggcctgctcc gacgcctctt tccatctcct tttactccaa
     2641 ttttcaactg aaaattgaga tatgtaattt taataaatat tatttgatta atcaaacaaa
     2701 taattgcagg caatagaatt acaagctttt tcctgcccgt cgggatttag ctctgctgat
     2761 atttaattac aaattacctg tccatccact ttttaaactc acctgctttt tcgcgggcaa
     2821 caatcaggtc gtcggcccgg aattcgggtt gaagctccag cttgaagcgg cttgaagaat
     2881 aactgaccag tttggttacg gcatcgatgt tgacgagata gtttcggtta atgcgaaaaa
     2941 actgatcggg atcgaccaac tgctgcaact gatcgaggga ataatcgaca tcgtaggttt
     3001 tgccctgaaa ggtgcggata aacaccgacc gctcgtcaat aaaaaacagc tcca
//
LOCUS       DAOPUW010000179         3051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_33083_length_3051_cov_3.088451, whole genome shotgun sequence.
ACCESSION   DAOPUW010000179 DAOPUW010000000
VERSION     DAOPUW010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33083_length_3051_cov_3.088451"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PKJ43_04055"
     CDS             complement(<1..603)
                     /locus_tag="PKJ43_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNZ71768.1"
                     /translation="MAKKNVLGRGLDALIEGADSVNQEHVAATSIDEIQISKIEANPW
                     QPRTTFDEEALQDLANSIKEVGIIQPLTLRKVSENKYQIIAGERRFRASKLAGLEKVP
                     AFIRESDDDRMLELALIENIQREDLDAIEISLSYQRLLDECQITQEELSERVGKKRST
                     VANYLRLLKLPAVIQKAIRDREISMGHARAIINVEDPETQI"
     gene            complement(636..1418)
                     /locus_tag="PKJ43_04060"
     CDS             complement(636..1418)
                     /locus_tag="PKJ43_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ71769.1"
                     /translation="MGKVIAIANQKGGVGKTTTAINLSASLAILEKKVLLIDADPQAN
                     ATSGYGFDLKKIENTIYECIVDRIDPETAILKTEVEKLELIPSHIDLVGAEIEMLKMP
                     NREKVLKEVIAPLKEKYDFIIIDCSPSLGVITVNALTAADSVIIPVQCEYFALEGLGK
                     LLNTVKLIQNKLNPELEIEGFLLTMYDSRLNLSNQVYEEVKRHFQNMVFNTIIQRNIK
                     LSESQGYGKPIVLYDAASKGAVNHLNLAQEIIDRNTIPVSAN"
     gene            complement(1552..>3051)
                     /gene="priA"
                     /locus_tag="PKJ43_04065"
     CDS             complement(1552..>3051)
                     /gene="priA"
                     /locus_tag="PKJ43_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNZ71770.1"
                     /translation="LRAGKQVLYLLPEIALTSQIIDRLKAVFGEKAGIYHSKFNDAER
                     VEIWNNVLHFDKDNNTNCQLILGARSSIFLPFENLGLIIVDEENETSYKQFDPSPRYN
                     ARDSAVMLGHFHGAKVVMGTATPSFDTFYNVQSGKYGYVHINKRYYDVAQPEIVIADI
                     SDAIKRRQMKSLFTPTLLNEISAALKKEEQIIIFQNRRGFSPYIQCRSCGWIPMCKHC
                     DVSLTYHKLQNNLQCHYCGYTVRLPNTCEKCQSTDIQSKGFGTEKIEDELKIFFPDAT
                     IERLDHDSTQGKHSHEKILHKFSTGKTNILVGTQMITKGLDFENVSVVGIIDADALLN
                     YPDFRSHERAFQLMLQVSGRAGRKKGRGKVVIQTYQASHPILSHVLTCDFNHLFSSTL
                     HERKLFQYPPFFRLVYITVKHKNRDRAQLASQQLTVELKKLENIAVLGPEYPLLARIQ
                     QYYQLMIRIKIRKSQSPVQIKCNILQSIDKVKHYEQNGSVVFAIDVDPL"
BASE COUNT          805 a          620 c          621 g         1005 t
ORIGIN      
        1 tatttgggtt tcaggatctt caacgttaat tatagcgcgg gcatgtccca tgctgatttc
       61 acggtctctt attgcttttt ggatcactgc cggaagcttc agcaaacgca aataattggc
      121 aacagtagag cgtttttttc caacccgttc gctgagttct tcttgagtaa tctggcattc
      181 gtcgagcaga cgctgatagc ttaacgagat ttctatagca tctaagtctt cgcgttgtat
      241 gttttcaata agtgccagct ctagcatgcg atcgtcgtcc gattctctga taaatgcagg
      301 aactttttca agtccggcaa gtttcgatgc cctgaacctg cgttcacctg caataatctg
      361 atatttattc tcggatactt tccgtaaggt gagaggctga ataataccaa cttctttaat
      421 ggagttggca aggtcttgaa gcgcttcttc gtcgaacgtt gtgcggggct gccatggatt
      481 ggcttcgatc ttcgatattt gaatttcatc gatcgatgta gcggctacat gttcctgatt
      541 tacactgtcg gcaccttcta tcaatgcgtc caatcctcgt cctaatacat ttttcttggc
      601 catacaatat ctgagtactt ttctgatttt actaattagt tcgctgaaac gggtatcgtg
      661 ttgcggtcga taatctcctg tgcaaggttc agatggttaa cagctccttt cgaggcagca
      721 tcatacaaaa caataggctt gccatatccc tgagactcac tgagttttat attcctttgg
      781 attatagtgt taaaaaccat attctggaaa tggcgtttta cttcttcgta aacctgattc
      841 gataaattaa gcctcgaatc gtacattgta agtaggaacc cttcaatttc aagttcagga
      901 ttaagcttgt tctgaatgag tttaacggta ttcaatagct tgcctaatcc ttcgagggca
      961 aaatattcgc attgaacagg aataattacc gaatcggcag cagtgagtgc attaacggtt
     1021 attaccccca acgacggtga acaatcaata attatgaaat cgtatttttc ctttaaggga
     1081 gcaattactt ccttcaaaac tttttcccgg ttgggcattt tcaacatttc tatttcagca
     1141 ccaaccaaat cgatatgcga cgggataagc tccagctttt caacttctgt tttgagaata
     1201 gcagtttcag gatcgatgcg gtcgacaata cattcatata ttgtattctc aatctttttc
     1261 aagtcgaacc catagcccga agtggcattg gcctgtggat cggcatcgat gagcaaaact
     1321 tttttttcca gtatcgcaag actggctgaa aggttaattg ctgttgtggt ttttcctact
     1381 ccacctttct gatttgcaat tgctattact ttacccattt gtattttttt tgacctccca
     1441 aaaataacat tttaacttgt attgacagca tatatttttg aacaacgaaa gttgttattg
     1501 actaacttgt tgctttacag ataaattcct ttgaagttta tctttctaac attacagcgg
     1561 gtcaacatct atcgcaaaca ctaccgagcc gttttgttcg taatgtttca ctttgtcgat
     1621 ggattgcaag atgttgcatt ttatttgtac cggtgattgg ctttttctga tttttatcct
     1681 tatcatcaat tggtaatatt gctggattct ggctagaagc gggtattcag gaccaagtac
     1741 agcgatattt tctaattttt taagctcaac ggtcagttgt tgagaagcaa gttgagcccg
     1801 atcgcggttt ttatgcttca ctgtaatata aaccagacgg aaaaatggag gatattgaaa
     1861 tagttttcgt tcatgtaatg tactggaaaa tagatggttg aaatcgcatg tgagaacatg
     1921 tgaaagaata gggtgggagg cctgatacgt ttgaatcact acttttcccc tgcctttttt
     1981 tcggcctgca cgaccactta cttgcaacat caattgaaaa gccctttcgt gcgatctgaa
     2041 atcaggataa tttaacagcg catcggcatc aataattccg accacactaa cattctcaaa
     2101 atcgaggcct tttgtaatca tttgagtgcc aacaagaata ttggttttcc cagtggaaaa
     2161 tttatgcaat atcttttcgt gagaatgctt gccctgtgtg gaatcatggt caagccgctc
     2221 tatggttgca tcagggaaaa agattttcaa ttcgtcttca atcttctcag taccaaatcc
     2281 tttcgattga atatctgttg actggcattt ttcgcaggta tttggcaggc gaacggtata
     2341 tccacagtaa tggcactgaa gattattctg tagtttgtga tatgtaaggc ttacatcgca
     2401 atgtttgcac atgggtatcc agccgcagct tcggcattga atataaggag aaaaacccct
     2461 gcggttttgg aaaataataa tttgctcctc tttttttagc gcggcactaa tttcatttaa
     2521 taaggttggg gtgaagaggg atttcatctg ccttcgcttt atggcatcag aaatgtcggc
     2581 gataactatt tcgggctgag caacatcgta ataccgctta ttgatatgaa catagccgta
     2641 ttttccgctt tgaacattat aaaaagtgtc gaacgacggt gttgccgttc ccattacgac
     2701 ttttgcccca tgaaaatgtc ccagcataac ggctgagtcg cgggcgttgt atctcggcga
     2761 cgggtcgaat tgtttgtaac tggtttcgtt ttcttcatcg acaataataa gccccaggtt
     2821 ctcgaacggc aaaaaaattg aagaccgggc tcccagaatt agttggcagt ttgtgttatt
     2881 gtctttatca aaatgcaata cgttattcca gatttctacc cgttcagcat cgttaaattt
     2941 tgaatgataa atgccagcct tttcgccaaa aacagcctta agccggtcaa ttatttgcga
     3001 agtgagcgca atttcgggca aaagatataa aacttgtttt cctgcacgaa g
//
LOCUS       DAOPUW010000180         3048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_33122_length_3048_cov_4.035750, whole genome shotgun sequence.
ACCESSION   DAOPUW010000180 DAOPUW010000000
VERSION     DAOPUW010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33122_length_3048_cov_4.035750"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="PKJ43_04070"
     CDS             complement(<1..1012)
                     /locus_tag="PKJ43_04070"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNZ71771.1"
                     /translation="MSIYKEAVKKPITTILIFVGVMVFGLFSLVQLPIDLYPEMELPA
                     ITIMTQYNGANASDIETNITKPVEDAMSSISDMKEVSSVSRDNLSLVFVEFEWETDLD
                     EAANDIRDALSLIENFLPEDAEDPTIFKFNSSMMPILFYSVTANESLDGLEKILDEKL
                     VNPLNKIDGIGSIGLSGAREREIRIEIDPVRLEAYNLTIEQIGNALAMENINMPSGNV
                     KMGKTDYPLRVKGEFVNSDEIKNIVVANYNGNIILLKDVADVNDTMREMNIEEKIDGK
                     DGARMYIMKKSGGNTVKIARDVRKELEKIKKTLPSDIQINTIFDSSEFIDSSIKNLSE
                     TLL"
     gene            complement(1035..2147)
                     /locus_tag="PKJ43_04075"
     CDS             complement(1035..2147)
                     /locus_tag="PKJ43_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNZ71772.1"
                     /translation="MKRILIYSIAILLFSACSNTGTPEAKQKKIDAINAKIRNLETKK
                     RSLIEATDTAGIDKVYPIKIEALASDTISKIVSFSTNLTAYEEVYLAPASPGKIEKIY
                     VKIGDRVKKGDILAKMDETQLNNSKLQLAQLETDYERMKALRETNSISAQQFEQIKTQ
                     VEVTRASVTYMAENTKLVAPFSGVITGKYFENGELYSGAPNTQAGKAAIVVLQQTDPL
                     KAIISISENYFPEIKVGTALDITSEIYPAETFNGIIERIHPTIDPLTRSFQVEVRVSG
                     NGKLRSGMFSKADLKLGEVASIIAPDNAIIQQEGTNIRYVFINENGVARRVNVTLGER
                     INDKIEIISEELKEGDELIVTGQSLLMNGSKVKVSQ"
     gene            complement(2160..>3048)
                     /locus_tag="PKJ43_04080"
     CDS             complement(2160..>3048)
                     /locus_tag="PKJ43_04080"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNZ71773.1"
                     /translation="VLDVKQNITNSYFMVLVTQKSMEIFEQNIADLKKIENHTRKMVD
                     TGVSEQTSLDQITVQVSMLENTKKTMERALRMGYNLLKFQLGVNAETDVILSESYDKL
                     LSENILQLPSDGNFSIESNLLYQMTETNQKISEKMVKMEQMAYAPTLTGFYAYNQKFL
                     TTGFDMTPNHIAGLSMSIPIFSSGSRAHKVALAKIKFEQAGINKAMVADQLSMQEQQL
                     KMDLNSAIENYELQKETVNVAKRVFDNVNRKFEQGMVSSLELTQSNSNYLQAETNFVQ
                     ASFTLLQARLALDKLYNKL"
BASE COUNT          731 a          613 c          618 g         1086 t
ORIGIN      
        1 ataacaacgt ttcagacagg tttttaatcg agctgtcgat gaattccgac gagtcaaaaa
       61 ttgtgttaat ttggatgtcg gaaggcagtg tttttttaat tttttcgagt tctttgcgta
      121 catcacgagc aattttaaca gtatttccgc ccgatttttt catgatgtac attcttgcac
      181 catctttacc atcgattttt tcctcgatgt tcatttcgcg cattgtatca tttacatcgg
      241 caacatcttt cagaaggatt atgtttccgt tgtaattggc aacgacaata tttttaatct
      301 catcgctatt tacaaactcg cctttaaccc tcaacggata atcggttttt cccattttta
      361 cgttacccga tggcatgttg atgttttcca tggccaaagc attcccaatt tgctcaatgg
      421 taaggttgta ggcctccagt cgaacggggt cgatttcgat cctgatttcg cgctcgcggg
      481 cgcccgataa cccgattgat ccaattccgt caattttgtt cagcgggtta accaacttct
      541 cgtcaaggat tttttccaag ccatccaaac tttcgttagc ggttaccgag tagaacagga
      601 tgggcatcat gctcgaattg aatttgaaga ttgttggatc ttctgcatct tcgggcaaga
      661 aattttctat caacgaaagg gcatccctga tgtcgtttgc cgcttcgtcg aggtcggttt
      721 cccattcaaa ttcaacgaaa actagcgaaa ggttgtcgcg cgataccgag ctaacttctt
      781 tcatatcgct gatcgacgac atggcatctt cgaccggctt ggttatattt gtctcaatat
      841 cagatgcatt agctccgttg tattgcgtca ttatggtaat tgccggcagc tccatctctg
      901 ggtagaggtc gatcggcagc tgaaccaacg agaacagccc gaaaaccatt acgcccacga
      961 agatcagtat cgtggtaatt ggttttttta ccgcttcttt atatatactc attgtattgt
     1021 atttcttttt taggttattg gcttacttta actttcgatc cgttcatcag caacgattgg
     1081 ccggtaacaa ttaattcatc tccttctttc agttcctctg aaattatttc gattttatcg
     1141 ttgatgcgtt cgccaagagt tacattaacc cgacgtgcta ctccgttttc gttgatgaaa
     1201 acataacgga tgttagttcc ttcttgctga attattgcat tgtcgggggc aattattgag
     1261 gcaacttcac caagtttaag gtcggcttta gagaacattc cagagcgcaa ttttccattg
     1321 cctgaaactc ttacttctac ttgaaacgaa cgggttagtg ggtcaatcgt tggatgaatc
     1381 cgctcaatta ttccgttgaa tgtttccgct ggatatattt cggaggtaat atcgagtgct
     1441 gttccaactt ttatttcagg aaagtaattt tctgaaatac tgattattgc ttttaatggg
     1501 tcagtttgtt ggagtaccac aatggctgct ttccctgctt gggtattcgg tgcgcctgag
     1561 tagagctcgc cgttttcgaa atatttgccg gttattactc cgctaaaagg agccacaagc
     1621 tttgtgtttt cggccatata tgttacactt gcacgggtca cttcaacttg ggttttaatc
     1681 tgctcgaact gctgtgccga aatactgttg gtttcacgca atgctttcat tcgttcgtaa
     1741 tctgtttcca actgagccaa ttgtagtttt gaattgttta actgggtttc atccattttg
     1801 gctaaaatgt cgcctttttt gacacggtcg ccgattttta cataaatttt ctcaattttt
     1861 ccgggtgatg caggggcaag gtatacttct tcgtaagctg taaggtttgt tgaaaaactt
     1921 acaattttcg atattgtatc gcttgcaaga gcttcaattt taatcggata aactttatcg
     1981 atgccagctg tatctgttgc ctctatgaga cttctctttt ttgtttcaag atttctgatt
     2041 tttgcattga ttgcgtcaat ctttttttgc tttgcttcgg gtgtgccggt gttgctacat
     2101 gctgaaaaca acagaatagc tattgaataa attagaattc gtttcatatt gtaatttttt
     2161 tagagtttgt tatataattt atcgagggct aaccgtgctt gcagtagagt gaaagatgcc
     2221 tgcacaaaat tagtctcggc ctgaaggtaa tttgaattcg actgggtaag ctcaaggctc
     2281 gataccattc cttgttcaaa ttttcggttc acattgtcga aaaccctttt ggccacgttc
     2341 acagtttctt tttgcagctc ataattttcg atagctgagt ttaaatccat tttcaattgt
     2401 tgttcctgca ttgaaagttg gtcggctacc attgctttgt tgattccggc ttgttcaaat
     2461 ttaattttgg ccagagcaac tttgtgagcc ctgcttccac tcgaaaaaat tgggatactc
     2521 atagagagac ccgcgatgtg gtttggtgtc atatcgaaac ctgtagttag gaatttttgg
     2581 ttgtaagcgt aaaaccctgt caatgttgga gcataagcca tttgttccat ttttaccatt
     2641 ttttccgaaa ttttctgatt tgtttcagtc atttggtaaa gaagattact ctcaatcgaa
     2701 aaattcccat cagatggaag ttgtaaaatg ttttcagaca gtaatttgtc gtaggactca
     2761 gagagtataa catcggtttc agcatttacc ccaagttgaa attttaacag attgtatccc
     2821 atccgaagcg ctctttccat agttttcttc gtattttcaa gcatgctcac ctgaaccgta
     2881 atttggtcga ggctggtttg ctcggagact cctgtatcga ccattttgcg agtgtggttt
     2941 tcgattttct ttaagtcggc aatgttttgc tcgaaaattt ccatggattt ttgagtgact
     3001 aaaaccataa aatagctgtt tgtgatgttt tgtttcacat cgagaact
//
LOCUS       DAOPUW010000181         3042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_33212_length_3042_cov_3.518246, whole genome shotgun sequence.
ACCESSION   DAOPUW010000181 DAOPUW010000000
VERSION     DAOPUW010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33212_length_3042_cov_3.518246"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2119)
                     /locus_tag="PKJ43_04085"
     CDS             complement(<1..2119)
                     /locus_tag="PKJ43_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ71774.1"
                     /translation="MNSIFIKLSFRKLLKSKVYSITNLVGLTIGFVAFTLIALYIQHE
                     TSWDKSHENYDRIYRIQRQMTNATQVVAGNDISPHTRAVTAELIEGKFPEFEKITVIR
                     EINSAFLSTNSEQMIHEKEGIHADNYFFDIFTYHFIEGNTKTALEQPYSVAISESLAQ
                     KLFNKTNVVGQTITFEKKYPLTVTGVYTDLPFNSSLRPDFIISFSTLKPMQKINRSDL
                     WSGDCMNYALLKPGASKQVAESKIKYLFNNYEELKYEELQLCPLSKVYLNFNDRSDYR
                     IILKLFGLIGIFILMMSGFNYINLSLAQTSMRGKEVAIKKVVGSRKHSVVIQFLTETI
                     GISLVALTLALAFSKLILPIFNNVVDKHISFHWVNDWSLVLILIAVALVTGLLSGLYP
                     ALFMASNKIVTLFKGNFFSGQRNQLGLKKALVTFQFTISLFLIILTASFSMQIKHISN
                     KDLGFNQEGLLYSTINISENKVQFDQFRDRLLQHPEIADVSISKNFPFVGQGGGMTNW
                     EGGNPDEEVICRFNQVSANYLSLLDAQITAGRNLSTDFGDESNNCVINETAVRYFGWD
                     NPIGKRVNNNRLIVVGVVRDFVYHDMHNPIEPTLMTLAPNSILGNWTFAFRVNESDVQ
                     TAKTIISNELTTAFPNDAFEITDFKMAFNNENSYRIYHSINKSLVFFTVLNVLLAILG
                     MFGLVSFSVARRTKEIGIRKINGS"
     gene            complement(2206..>3042)
                     /locus_tag="PKJ43_04090"
     CDS             complement(2206..>3042)
                     /locus_tag="PKJ43_04090"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNZ71775.1"
                     /translation="ICAMRIDNNYLKTMGIKIKEGTDFPDYYEKVLTNVDTSMTYQVI
                     LNQEAIRRMGMKDPVGKPFKAWNYYNGSIVGVTEDFHFESLHQNVEPLLLLPLTENPG
                     YIIVRINANNFSQTIDKIKSAWSKVVPQSNCEIGFFDNRLASLYESEVKISGLFKYFS
                     FIAIFISCIGLFGLSLFIIERRKKEVGIRKVNGANTPEVMLLLNKDFALWVIISIVIA
                     TPVAWFIMHRWLENFACKTTLSWWIFALSGLLALGIALLTVSWQSWRAATRNPVKALR
                     YE"
BASE COUNT          849 a          650 c          638 g          905 t
ORIGIN      
        1 tgctgccgtt gattttacgg attccaattt ccttggtgcg ccgtgcaaca gagaatgaaa
       61 ccaaaccaaa catgcccaaa attgcaagca gcacgttcaa tacggtaaaa aacacgagcg
      121 atttgttgat tgagtgatat atacgatagc tgttttcgtt gttgaacgcc attttgaaat
      181 cggttatttc gaaggcatcg ttgggaaatg ccgtagttaa ttcattggaa ataattgttt
      241 ttgcggtctg aacgtccgat tcgttaaccc tgaatgcaaa ggtccagttt cccagaatgg
      301 aattgggagc caaggtcatc aatgtgggtt caatagggtt gtgcatgtcg tggtaaacaa
      361 aatcgcgaac cacacctaca acgatcaagc ggttgttgtt cacgcgcttg ccaatggggt
      421 tgtcccatcc aaaatagcgt acagctgtct cgttgatcac acaattgttg ctctcgtctc
      481 caaaatctgt cgacaagttg cggcctgcag taatttgggc atccagcaac gataggtaat
      541 tagcactcac ctgattgaat cggcaaatca cttcctcatc cgggtttccg ccctcccaat
      601 tcgtcattcc gccaccttgg cccacaaagg ggaaattctt cgaaatggaa acgtctgcaa
      661 tctcgggatg ctgtaaaagc cggtctctaa actggtcgaa ttgcaccttg ttttccgaga
      721 tgtttatcgt cgaatagagc aagccttctt ggttaaaccc caagtctttg ttcgaaatgt
      781 gcttgatttg catcgagaac gaggccgtta aaataatcag gaacaacgaa atggtaaatt
      841 gaaaggtcac caaggctttt tttagcccca gttggttgcg ttgacccgag aagaagttcc
      901 ctttaaacag ggtcacaatt ttgttcgagg ccataaacag tgcaggatac aagcccgaaa
      961 gcaagcccgt taccaaggct actgcaatca atattaacac caggctccaa tcgtttaccc
     1021 aatgaaacga gatgtgcttg tccactacgt tgttgaaaat cggaaggatc aatttgctga
     1081 acgccagagc caaagtcaaa gctacaagtg atatgccaat ggtttcggtc aagaactgaa
     1141 taacaacact gtgtttgcgg cttccaacca cttttttgat ggccacttct ttgccgcgca
     1201 tcgaggtttg ggccagcgaa aggttgatat agttgaagcc cgacatcatt aaaatgaaaa
     1261 tcccaatgag accaaatagt ttcaatataa tgcgatagtc gctcctgtcg ttaaagttga
     1321 ggtacacttt cgaaaggggg cacaattgca gttcttcgta tttaagctct tcgtaattgt
     1381 tgaacaggta tttgattttc gattcggcca cttgtttcga agccccgggc tttagcaggg
     1441 cgtagttcat gcaatcgccc gaccacaagt ccgatcgatt gattttttgc atgggcttta
     1501 aggttgaaaa ggagataata aagtcaggtc tcaggcttga attgaagggc aaatcggtat
     1561 acacgcccgt tactgtcagc gggtatttct tctcgaaggt gatggtttgc cccactacat
     1621 tggttttatt gaaaagtttt tgcgccaatg attcagaaat ggccaccgaa tagggctgtt
     1681 ccaaagccgt tttcgtgttt ccttcaataa aatggtaggt gaaaatatcg aaaaagtagt
     1741 tgtcggcgtg gatgccctct ttttcgtgga tcatttgttc ggaatttgta gaaaggaagg
     1801 ccgaattgat ctcgcgaata acggttattt tttcaaattc aggaaatttc ccttcgatta
     1861 gttctgctgt tacagcccgt gtatgcggcg aaatgtcatt cccggcaacc acctgggtag
     1921 cattggtcat ttgtcgttgt atgcggtaga tccggtcgta gttctcatgg cttttgtccc
     1981 agcttgtttc gtgctgaatg tatagcgcaa tgagcgtaaa tgcaacaaaa ccaatggtaa
     2041 ggcccacaag gtttgtgata gaatacacct tgcttttcag tagttttcga aacgacaatt
     2101 taataaaaat tgaattcatg gctatattgt aaacttcatt tgttattttc tatttcaatg
     2161 cacaaatctt caactctggc tttttttgtt tttctttttt ggaacttatt catacctcag
     2221 cgccttcacc gggttcctcg tagcagcacg ccagctttgc cagctcactg ttagcaaagc
     2281 aattcccaat gccagcaaac ctgacaatgc aaaaatccac cagcttaaag ttgttttaca
     2341 ggcaaagttt tcgagccagc ggtgcataat aaaccatgca acgggtgtgg ctataacaat
     2401 ggatatgata acccataaag caaaatcctt atttagtaac aacattactt ccggtgtgtt
     2461 ggctccatta acttttctaa ttcccacctc ttttttcctt ctttcaatta taaacagcga
     2521 taatccgaac aagccaatac acgagatgaa aatggcaatg aaagagaagt atttaaacaa
     2581 tcccgaaatt ttcacctccg attcataaag ggatgccaaa cggttgtcaa aaaagccaat
     2641 ttcacaattc gattggggta ctactttcga ccatgcactc ttaattttat caattgtttg
     2701 tgagaagttg tttgcattga tgcgcacaat aatgtaaccg gggttttcag ttaacggcaa
     2761 taaaagcaac ggttcaacat tttgatggag cgattcgaaa tggaaatctt cagtaacccc
     2821 aacaattgat ccgttataat aattccaggc tttaaacggt ttccccacag gatctttcat
     2881 gcccattctt ctgattgctt cctggtttag aatcacctga tacgtcatgc ttgtatcaac
     2941 atttgttaaa actttttcat aataatcggg aaaatcagtt ccttccttta tcttaattcc
     3001 catggttttg aggtaattat tatcgatgcg catggcacaa at
//
LOCUS       DAOPUW010000182         3041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_33224_length_3041_cov_3.579705, whole genome shotgun sequence.
ACCESSION   DAOPUW010000182 DAOPUW010000000
VERSION     DAOPUW010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33224_length_3041_cov_3.579705"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PKJ43_04095"
     CDS             <1..679
                     /locus_tag="PKJ43_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ71776.1"
                     /translation="VAKAFGDKLLFEDLEFSLPPNGIVGVIGPNGAGKTTLFRMIMNL
                     ESADKGEFNVGETVVIGYADQTHTAIDPEKTVYQVVSGGQDDIMVGGRLINARAYVSK
                     FNFSGSDQEKKCGVLSGGERNRLHLALTLKSGANVLLLDEPTNDIDVNTLRALEEGLE
                     SFAGCAVVISHDRWFLDRIATHILAFEGDSQVKFFEGSFSEYEENKKKRVGDITPHRI
                     KYRKLVK"
     gene            689..1696
                     /locus_tag="PKJ43_04100"
     CDS             689..1696
                     /locus_tag="PKJ43_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate delta-isomerase"
                     /protein_id="HNZ71777.1"
                     /translation="MTERKMAHINLAARSIPESLIPDQRFNYEPLLSGHTQHSSKYSF
                     LGKSLGHPLWVSSMTGGAKDAGVINHMLAEACAEFGLGMGLGSCRALLQSDEYFDDFN
                     LRPVIGFELPFYANIGIAQIEELLLNNELSRIDDLVGEKLNADGLIIHVNPAQEWLQP
                     EGDVFLIPPLETIERFIDHLGHRYKLIVKEVGQGFGPQSLKKLFRTEIDAIEFAAFGG
                     TNFVNIEIERKRSDHAQYMYPMGNVGHTAEEMLEFVNSQLADCSVCNKSLIISGGIKN
                     FLDGFYLVSKSKLPSTYGMAFRLLEHAMISKKDLFDFISAEIDGFRFASKFLTIRESV
                     E"
     gene            2079..>3041
                     /locus_tag="PKJ43_04105"
     CDS             2079..>3041
                     /locus_tag="PKJ43_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71778.1"
                     /translation="MKLLSRFIWLLIAIGAIVFIGVSLVNFRAENKFKEIKTSISQEY
                     NVLIDKMLSPDRSEISTTYNYGIINASTTRSFLSGSIPVPELLAFDLDTMVMNHFLVD
                     AIWFYQSSGNQFYFYSHHGVGKEVLSISASDINALFSNTKTSSFYVKNNGKLYRVFGS
                     KVGDESYNFGFVFSASIQDDRWVDIYQKEINNSEAKFSGVETPLPEVPKEYILIERPL
                     LSYNGKPAENLLITLKLPFLSLWKSTTTTDNWLMSGAIGLIVIFLTLSLLLWVISPLK
                     RISKSLEKGDSHDIQPLIKNTSEMGKVARMISDYHQKNEEIEASE"
BASE COUNT          898 a          511 c          664 g          968 t
ORIGIN      
        1 tgttgccaaa gcttttggag ataaactact gttcgaagat cttgaattta gccttccgcc
       61 taacggtatt gttggggtta ttggccccaa tggagcagga aaaacgacgc ttttcagaat
      121 gataatgaac ctggagagtg ctgataaggg cgaatttaat gttggcgaaa ctgttgtgat
      181 tggttatgcc gatcaaaccc atactgctat cgatcctgaa aaaacagtat atcaggtagt
      241 ttcgggtgga caggatgata taatggtagg tgggcgtttg attaacgcaa gggcctatgt
      301 atcaaaattt aattttagtg gttccgatca ggaaaaaaag tgtggagtac tctctggtgg
      361 tgaaagaaac cggttacatc tagccttgac tttaaaatcc ggagcgaatg tgctgctact
      421 cgatgaacca acaaatgaca tcgacgtaaa cactttacgt gcacttgaag agggccttga
      481 aagctttgcc ggttgtgcag tagtaatttc gcacgaccgc tggttcctcg atcgtatagc
      541 tacacatatt cttgcttttg agggcgattc gcaggttaaa ttctttgaag gttctttctc
      601 tgaatatgag gaaaacaaga aaaaaagagt gggtgatatt actccccatc gtataaaata
      661 tcgaaaactg gttaaatagc atgatacaat gacagaacga aaaatggctc atataaatct
      721 ggcagccaga tctatacctg aatcgttgat cccagaccag cggttcaact atgagccttt
      781 gctttctggt catacgcaac atagctctaa gtattctttt ttgggcaaaa gcctggggca
      841 tccgttgtgg gtatcgagta tgactggagg agcaaaggac gcgggtgtta ttaaccacat
      901 gctcgcagag gcttgtgctg agttcggtct tggtatgggc ctaggttctt gtcgtgcact
      961 tttgcaaagt gatgagtatt tcgatgattt caatttgcga cctgttattg ggttcgaact
     1021 tcccttttat gcaaatattg gcattgccca aatagaggaa cttttgctga ataacgaatt
     1081 atcccgtatt gatgatttgg ttggtgaaaa gctaaatgcc gacgggctga ttatccatgt
     1141 taatcctgct caggagtggt tacaacccga gggcgatgtg ttcttgattc cccccttgga
     1201 aacgatagaa cgttttattg atcatcttgg gcatcgatat aagttgattg tgaaggaggt
     1261 ggggcagggc tttggtcctc agagtcttaa gaagctattc agaacggaaa ttgatgcgat
     1321 tgaatttgct gctttcggag gaactaattt tgtaaacatt gaaatagaac ggaaaaggtc
     1381 tgatcatgct caatatatgt atccaatggg gaatgtgggt catactgcag aggaaatgct
     1441 cgaatttgta aacagtcaac ttgctgactg ttctgtttgt aataaaagtc tgataatctc
     1501 gggagggatc aagaattttc tggatggttt ttatcttgtc agtaaatcga aattgccgtc
     1561 gacttatggt atggcttttc gattattgga acatgctatg atttcgaaaa aagatctttt
     1621 tgattttatt tctgccgaaa ttgatgggtt tcgttttgca tcgaaatttc ttacaataag
     1681 ggagtcggtg gagtaattac ttgaatttca aaaatatata ttaaagaaat tcaaacacga
     1741 ttacttaatg agtttttgga tataagtatt tgaaatttaa tttattgaag ttgttttatt
     1801 cttgtgtgca atgcattttg agtttgttaa agttaaatgc atgatttgaa aataacgttg
     1861 gtcctgacga ttgatagaac agttgaaaaa ccttcatcaa ttgaaagatt caatattatt
     1921 caaccaatca tcaataataa ctttttttct tttaactaat ttcttaactt tcatctcaat
     1981 tcttgagaat aagttgccat atttgatcga gatggtttct ataaatttgg taattttgat
     2041 ttcaaagaaa aggaataact ggttttaagg gatacattat gaaacttctt tcacgcttta
     2101 tttggctctt aatcgcaatt ggagcaattg tgtttatagg ggtgagcctt gttaatttca
     2161 gggcagaaaa caagtttaag gagattaaaa catcgattag tcaggaatat aacgttttga
     2221 ttgataaaat gttgtctccc gatcgatcgg agattagcac cacgtataat tatgggatca
     2281 taaatgcatc aacaacccgt tcatttctaa gcggatcgat tcctgttccc gaactgctgg
     2341 catttgacct tgacacgatg gttatgaacc actttcttgt cgatgccatt tggttttatc
     2401 aatcttccgg gaaccagttt tatttttatt ctcaccatgg cgtcggaaaa gaagtgttgt
     2461 cgatcagtgc gtctgatatt aacgctttgt ttagcaatac aaaaactagt agtttctatg
     2521 ttaaaaacaa tggaaaacta tacagggttt tcgggtctaa ggtcggtgac gaatcatata
     2581 attttgggtt cgttttttct gctagtattc aagatgaccg ttgggtcgac atttaccaaa
     2641 aggaaatcaa taactctgaa gcaaaatttt caggcgtcga gaccccactg cccgaagtac
     2701 ccaaggagta tattttgatt gaacgaccat tactttcata caatggtaaa ccagccgaaa
     2761 atctgcttat taccttaaaa cttccattct tgagtttgtg gaaaagcacc actactaccg
     2821 ataactggtt gatgagcgga gctattggat tgatcgtgat ttttctcaca ttatcacttt
     2881 tactttgggt aatatctccg ttgaaaagaa tctcaaaatc gcttgaaaaa ggagattcgc
     2941 acgatattca acccctgata aaaaacacat ctgaaatggg taaggttgcc cgcatgatta
     3001 gcgattacca tcagaaaaat gaagaaattg aagccagcga a
//
LOCUS       DAOPUW010000183         3036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_33311_length_3036_cov_2.899698, whole genome shotgun sequence.
ACCESSION   DAOPUW010000183 DAOPUW010000000
VERSION     DAOPUW010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33311_length_3036_cov_2.899698"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3036
                     /locus_tag="PKJ43_04110"
     CDS             <1..>3036
                     /locus_tag="PKJ43_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNZ71779.1"
                     /translation="AIYSTQVNYINPEEIKLLEEVASDISYALDALETAAEQKKAEVM
                     AHERLKELNAFYKLSELTENRALTSDELCAEFVNILPQSLKYNHSAYAKIEIQDKIFV
                     SDNYIKTTNWALQDPIFTNGIRTGKIEVGYNAQMPVEDDGSFLKEEHLLINGIAERLG
                     HIIERKQAEVQILKSESQFRNLMNQMQLGMVLHEIILNDQEQPVDYRFLDCNPAYEQL
                     TGLNRSEILGKTVLEVLPNTEKIWIENFGKVALSGVPIMFESYARELNRHYSVKAYQP
                     APMQFAVLTEDITEQRINLQKIESNSLKFKYLSRSAAEMLELKNLDSLYEYITKSLHD
                     QYPDTIILFQNVNHAQHTSKLTHIEGVSEKIKKMAIKLAGSQIYNKEFELIPEHLEFY
                     MSGKLHHFEKGLADFVGSQFPRFAASTLEKAVGIKQIYTIGITKDDVLHATIHLFNRG
                     AVPITDHEYIESFIKQAGIVIDRAKTAELLAESEEKYRLITENASDVIWVLNINTRRF
                     TYISPAVMQLRGFTPKEAIAQTIEEAVTPESLIHIQQALEANLGKFMTNQLDMEYYMD
                     EIQQPCKDGRIIWVEVSTTFRTNKQGEIEVVGVSRNIDERKKLEAAIKVNEQKFRLLF
                     ENSPLGIYIANTSGEILDANPALISILGSPSLEMTKKINVLEHKKLQENGYTQQFLKC
                     IETKKVVTIELPYTAHWGKKVYLSCYLVPMTNAQGSVEKIYTLMEDITLRKQNEEELI
                     KLSLVAKQSPVSIVITNLDGNIEYVNSKFTEVTGYLPEEVIGQNPRILKSGDTPQEGY
                     KSMWETITSGNEWSGIFHNKRKNGDLYWEKANILPVKTADGNPLYFLALKEDITEQLK
                     AQDALIESETLLKEALAAKDKFFSIISHDLRSPFSTLVSFTELMSDDQTHFSVDEYKQ
                     YSRAIFKTAQSTYSLLENLLEWSRLQRGSIPFKPESIDINEFIKTCDPSIIEKMNAKQ
                     IEFEIQVEDNLTVQADKNMLHAILRNLMSNATKFTPLGG"
BASE COUNT         1056 a          660 c          602 g          718 t
ORIGIN      
        1 agctatttac tcaacccaag tcaattatat taacccagaa gaaatcaaac tcctcgagga
       61 agttgcttct gacatcagct atgcattaga tgcccttgaa accgcagccg aacaaaaaaa
      121 agcagaagtg atggcacatg aaaggctaaa agaactgaat gccttttata aactttctga
      181 attaaccgaa aacagggcct taacttcaga tgagctttgt gccgagtttg taaatatttt
      241 acctcagagc ttgaaataca atcattcagc ctatgccaaa attgaaattc aagataaaat
      301 atttgtatcc gataattata taaaaacaac caactgggct ttgcaagatc ccattttcac
      361 gaatggtatt cgaaccggga aaattgaagt gggctataac gcgcaaatgc ccgtagaaga
      421 cgatggctcg ttcttaaaag aagaacattt gttgattaat ggtattgccg aacgccttgg
      481 gcacataata gaacgcaaac aagccgaagt acagatattg aaaagcgaaa gccaattccg
      541 gaacttaatg aaccaaatgc agttagggat ggtattgcac gaaatcattt taaacgacca
      601 agagcaaccg gtcgactacc gttttttaga ttgtaaccca gcatacgagc aacttacagg
      661 tctaaaccgc tcggagatat tggggaaaac cgtactggaa gtactaccta acaccgaaaa
      721 aatttggatc gaaaacttcg gcaaggttgc attatctggc gtacctatta tgttcgaaag
      781 ttatgcccgt gaactcaacc ggcattacag tgtaaaagcc tatcaacctg cacccatgca
      841 atttgcagta ttgaccgaag acattaccga acagagaatc aacctacaga aaatagaaag
      901 taatagttta aaattcaaat atttaagtcg ttcggccgct gaaatgctcg agcttaaaaa
      961 cctcgattca ttgtatgaat acataaccaa atcgttgcac gatcaatatc ccgacaccat
     1021 tattcttttt cagaatgtga accatgccca acacacttcg aagctcaccc acattgaagg
     1081 agtatcggaa aaaataaaaa agatggcaat caaactggca ggttctcaaa tatataacaa
     1141 agaatttgaa ctgattcccg agcatttgga attctacatg tcgggcaagc ttcatcattt
     1201 cgaaaaaggg ttagccgatt ttgtaggaag ccaatttcca cgatttgcag cttcaacgct
     1261 tgaaaaagca gttgggatca agcagatcta tacgattggg atcaccaaag acgatgtgct
     1321 tcatgcaacc attcatttgt tcaaccgtgg ggcagtcccc ataaccgatc acgaatacat
     1381 tgaatcattc atcaaacaag ctggcattgt gattgatcga gcaaaaactg cggagttgct
     1441 ggcagaaagc gaagaaaaat accgtttgat taccgaaaac gcttcggacg ttatttgggt
     1501 actcaacatc aacacccgga ggttcaccta tataagtccg gcagtaatgc aactgcgcgg
     1561 ttttaccccc aaggaagcca ttgcacaaac aattgaagaa gcagttacgc ccgaatcgtt
     1621 gatacacatt cagcaagccc tcgaagcaaa cttgggaaaa tttatgacaa accaactcga
     1681 catggaatac tacatggatg aaatacagca accatgtaag gatgggcgta ttatctgggt
     1741 cgaagtttca accacattca ggaccaacaa acaaggagaa attgaggttg taggtgtaag
     1801 tcgcaacatc gacgagcgca aaaaactcga agcggcaatc aaagtgaacg aacagaaatt
     1861 caggcttttg ttcgagaatt cgcctttggg catttacatt gccaacacaa gtggcgaaat
     1921 tctggacgcc aacccagccc tgatctccat tttaggttca ccttcgctcg aaatgaccaa
     1981 aaagataaat gtacttgagc acaaaaaatt gcaagaaaac ggatatactc aacaatttct
     2041 aaaatgcatc gagaccaaaa aggttgtcac aattgaacta ccatacacag cacattgggg
     2101 caaaaaagtt tacctatcgt gctacttggt gcccatgaca aacgcacagg gcagtgtgga
     2161 gaaaatctac acgttgatgg aagacattac cctgcgcaaa cagaacgaag aagaattgat
     2221 caaattgtcg ttggttgcca agcaaagccc tgtaagcatt gtcattacca atttggatgg
     2281 taacatcgaa tatgtcaatt caaagttcac cgaggtaacg ggttatttgc ccgaagaagt
     2341 aataggtcaa aacccacgca tcttaaaatc gggcgacaca cctcaagaag gatacaaatc
     2401 gatgtgggaa accattactt cgggcaacga atggagcggc atttttcaca acaaacggaa
     2461 aaatggcgat ctgtattggg aaaaggccaa tattcttcca gttaaaactg ccgatggaaa
     2521 tccattgtac tttctagcac tcaaagaaga tataacagaa caactgaaag cgcaggatgc
     2581 tttgatcgaa agcgaaactc ttctaaaaga ggctctagca gccaaggata agttcttctc
     2641 cattatttcg cacgacctac gcagcccatt ctcaacactc gtaagcttta ccgaattgat
     2701 gagcgacgat cagactcatt tttcagtcga cgaatacaag caatattcac gggcaatttt
     2761 caaaacggcc caatcgacct acagcctact cgaaaacctg ctcgaatggt ctcgcctgca
     2821 aaggggtagc attccattca agcccgaatc tattgacatc aatgaattta ttaaaacatg
     2881 cgatccatcc ataattgaaa aaatgaatgc caaacaaatc gaatttgaaa ttcaggttga
     2941 agacaactta acagttcagg ccgataaaaa tatgctacac gccattctgc ggaacctgat
     3001 gtcgaatgca accaaattca ccccattggg aggaaa
//
LOCUS       DAOPUW010000184         3031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_33408_length_3031_cov_3.348454, whole genome shotgun sequence.
ACCESSION   DAOPUW010000184 DAOPUW010000000
VERSION     DAOPUW010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33408_length_3031_cov_3.348454"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PKJ43_04115"
     CDS             <1..263
                     /locus_tag="PKJ43_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNZ71780.1"
                     /translation="DSAAIYTQQNRADLADAEMAQVAVFNSYLPAMMSDEELEKALVE
                     IIKNSGAEGVKDMGKVMGIATKELAGKADGKAISQKVRSLLQ"
     gene            532..>3031
                     /locus_tag="PKJ43_04120"
     CDS             532..>3031
                     /locus_tag="PKJ43_04120"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ71781.1"
                     /translation="MCKPLLLLVSLLLVFSVNGQVPKSVAVDQRYGNLTFSQFAKKLF
                     EQDSVRIFYQEKWVENITTPTVTQKVLLSDFLQTVLSQSDLHYIDFQGNIVVMPGRQQ
                     VAQRAITQNTVIVGNPLDKGKYRTAVISGQVFEGKTKNPIPGAQIICEALSKGTSTNF
                     DGKFTLELPTGQHKIKFSFMGLDDEFRDVFVYSDGSMEVDLYEKSISLNQINIFADRP
                     EDNFRSTSMGMVKLSSKSIKKLSVLMGEPDIIKSMVLLPGVQSAGENSSGFNVRGGNT
                     DQNLILIHDAPVYNTAHMFGLFSMLDPGVVDNVTLYKSGIPSKFGGRLSSVMDIDVRK
                     GQLDKFKVNGGIGLINSRLSVEGPIYKNKISFMAGGRATYSDWMLGLLKNYQLQQSSV
                     SFYDFNAKIDYEINSNNRVSLFGYGSHDYFNYFEEAEYGYGNVIGSARWNHIFDKFTS
                     ATLSLNFSKYTADLVDYTSKNYEYDLSTSIEQEQVSYHFSTSRFARHRISAGMNVIRY
                     LIEPGNSAPHSPLSAAAFINVDDENGYEAALYLEDEFDLTPELALMAGLRYSGFAKVG
                     QAQVKTYYPGQPLNESTVSGMNEFGAGQVVKFHHGAEPRIALRYELPQSSSLKLGYNR
                     TMQYIRQISNSTSITPADYWKSSDTYLKPLVANQLALGYFKNFKNNKYETSLELYYKN
                     IENEVDYKNGGRLVLNRNLEQVLISGIGRAYGLELMVKKSSGDLTGWIAYTYSRSFKK
                     MNGAFDEEKVNGGKWYRSNIDKPHDLTMVMNYKLSRRFTFSSNFTYSTGRPVTLPEQK
                     YNVGSYEIVYYSERNKYRMPDYHRLDLALTYEGSL"
BASE COUNT          852 a          574 c          686 g          919 t
ORIGIN      
        1 ccgattctgc agcgatttat acccagcaga accgcgccga ccttgccgat gctgaaatgg
       61 cacaagtggc tgtttttaac agctatttgc cagctatgat gagcgatgaa gaattagaaa
      121 aagccttggt ggaaattatt aaaaattctg gtgccgaggg tgttaaagat atgggaaaag
      181 tgatgggcat tgccacaaaa gagcttgcag gaaaagccga cggcaaggcc atttcacaaa
      241 aggtacgttc actgttgcaa taaaagttta aaatttaaac ttttggggta agcgtcgcgg
      301 ggaaacttgc ggcgcttttc tatttcttct tttattggct ataatctcaa cacgcaagaa
      361 ttctttttgg gcgaatcagt actgttttat tgaatcatta attgatatcc attttctact
      421 gctatctgta acataattgg tgattgtttc gtaacttcag gggctttatt ttcaagttgc
      481 aaaagtttgg tgatttgaag gtaatttttt aaacgaacaa aacctttaac tgtgtgtaaa
      541 cctttacttt tgcttgtctc tttgttgctg gttttttcag tgaatggaca agttcctaaa
      601 tctgttgctg ttgaccaacg ttacggaaat ctcacttttt cgcaatttgc taagaaatta
      661 ttcgagcagg attctgttcg tattttttat caggaaaaat gggtcgaaaa tattaccacg
      721 ccaacagtaa cgcaaaaagt tctgctttcc gattttttgc aaaccgtact ttcacaatca
      781 gatttacatt atatcgattt tcagggaaat attgttgtta tgcctggccg acagcaagtt
      841 gctcaacgtg caataacaca aaatacggta attgttggta atcctctcga taaagggaaa
      901 tatcggactg ccgttatttc gggacaggtt ttcgaaggga aaacaaaaaa tcctatccct
      961 ggtgcgcaga ttatttgcga agctctttca aagggtactt caacaaattt cgacggaaaa
     1021 tttacgttag aactccctac tggtcagcat aaaattaagt tttcatttat ggggcttgac
     1081 gacgaatttc gcgacgtttt tgtttatagc gacgggagca tggaggtcga tttgtatgaa
     1141 aaatcaatct ctctcaatca gataaatatt tttgccgatc ggcccgaaga caatttccgg
     1201 tctacttcaa tgggaatggt caagctttct tcaaagtcga taaagaaact atcggtactg
     1261 atgggtgaac cggatatcat aaagagtatg gttttgctgc cgggagtcca atcggctggt
     1321 gaaaactcgt cgggctttaa tgttcgtggc ggaaataccg accagaacct gatcctgatt
     1381 cacgatgccc cggtttacaa cacggcgcac atgttcggcc ttttttcaat gctcgatccg
     1441 ggggtggtcg ataatgtaac tctttataaa tcggggattc cctcgaagtt tggtggccgg
     1501 ttgtcgtctg ttatggatat tgatgttcgc aaaggtcagt tggataaatt taaggtgaat
     1561 ggtggaattg gtttgattaa tagccgattg tctgtcgaag ggcctatcta taaaaataag
     1621 atatcgttta tggccggagg tagggcaacc tattctgatt ggatgcttgg attgttgaaa
     1681 aattaccagt tgcagcaaag ttccgttagc ttctatgatt ttaatgcaaa aatcgattat
     1741 gagataaact caaacaatcg ggttagcttg ttcggatatg gtagccacga ttattttaac
     1801 tattttgaag aagcagaata tggttacggg aatgtaattg gctctgctcg ttggaaccat
     1861 atttttgaca agtttacatc ggccacgcta agtttgaact tcagcaagta tacagccgat
     1921 ctggttgact atacctcaaa aaactatgaa tacgaccttt caacttcgat agaacaagaa
     1981 caggtttcct atcatttttc aacctcccgg tttgcgcgtc accgcatttc ggctggtatg
     2041 aatgttattc ggtaccttat cgagccggga aattcagcgc cacattcccc gctttctgcg
     2101 gctgctttta tcaatgttga tgatgaaaac ggatatgaag ctgcccttta ccttgaggat
     2161 gaattcgacc tgacccctga gttagccctt atggctgggc tgaggtattc gggttttgcc
     2221 aaggttggac aagcgcaggt aaaaacatat tatcccggtc aaccgctcaa cgaatcaacg
     2281 gtttcgggca tgaatgaatt tggagctggg caggtcgtga agttccatca tggagcagaa
     2341 cccagaattg ctttacggta tgaattaccc cagtccagtt ctttgaaact cggctataac
     2401 cgtaccatgc agtatataag gcaaatatcg aacagcactt caattactcc tgccgattat
     2461 tggaaatcga gcgacacata tctgaaaccg cttgttgcta accaactggc gttggggtat
     2521 ttcaaaaact ttaagaacaa taaatacgaa acctcactcg aactctatta taagaatatt
     2581 gaaaatgagg tggattataa aaatggggga cgtttggtcc taaaccggaa tcttgagcaa
     2641 gtgcttatct ccggtattgg tcgtgcgtat gggcttgagt tgatggtcaa gaaatcgtcg
     2701 ggcgatttaa caggttggat tgcctatacc tattcgcggt cgtttaagaa aatgaatggc
     2761 gcttttgatg aagaaaaagt gaacgggggt aaatggtacc gttctaatat cgacaagcca
     2821 cacgatttaa caatggttat gaactacaag ctttcgcgga ggtttacttt ttcatcgaat
     2881 tttacttaca gcacgggtcg tccggttact ttgcccgagc aaaaatataa tgtggggtcg
     2941 tatgagattg tatattattc agagcgaaac aaatatcgca tgcccgatta tcatcgcctc
     3001 gatctggccc ttacgtatga aggaagtttg t
//
LOCUS       DAOPUW010000185         2999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_33934_length_2999_cov_3.272079, whole genome shotgun sequence.
ACCESSION   DAOPUW010000185 DAOPUW010000000
VERSION     DAOPUW010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33934_length_2999_cov_3.272079"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1915)
                     /locus_tag="PKJ43_04125"
     CDS             complement(<1..1915)
                     /locus_tag="PKJ43_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNZ71782.1"
                     /translation="MFIMILLLSVVSITYAQERLTQGVIIDESGIPIPGANILIKGTS
                     NGTVSDSDGKFTVKIPSDNTTLVISFVGYEMQEVQVGNTAAITVVLKELRSEIDNVIV
                     IGYGTQKKSDLTGAIVSVSGEQLSKTPSSGALQALEGKAAGVQIINSSGMPGAPIYVR
                     VRGINTITKQSQYGGVAGPVYIIDGIQGDINTINPNDIDHIEILKDASAQAIYGSSGG
                     NGVIIVTTKLGKKNQKTKVDFSMYRGIQTNDISVDMCNTEEFIKIYNSLDATKPINRI
                     TANPDSLPNTNWWNEISSNAVMEEYNLSVSGGSENSASYLSLGYFNQDGVVDKTNYKR
                     YTIKVNNSFDINKRIRIGENISLTATRNQGNDGWGTPMGSINQSPISYVRDTSSTLTP
                     EQMKTRNIGWGGWAQPMFNTGNGNPVAGIYYYNAKKGTYAMNGNLFANVEIIKGLTYT
                     NNFGFGVNFYEMDDFKPYYFINTTQNNKVIQVSRQLDRTFKWNWQHVLDYKMTLNNQH
                     SIDVMAGFEASEWFYKGLSGQADSLLTNGATPEYQYIDATLRDYGSEYSYAGGGMGHG
                     SKYAYFGRLNYEYKNLFLAQFTYRYEGSTNFGKDHRFGGFPGFSAGFKFSELNAVKEN
                     IPYLSFGKLRFGWG"
     gene            complement(2057..>2999)
                     /locus_tag="PKJ43_04130"
     CDS             complement(2057..>2999)
                     /locus_tag="PKJ43_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNZ71783.1"
                     /translation="NKDSYWATDDSVTTASLTIDFEKPTSFNRFMAQEYIRLGQRVKS
                     FTVEANVGGEWKEITKGTTIGYKRILRFPTVEATQLRFNITSSKACPLISNIGVYNAP
                     QILPDPSIIRNQLGEIIIVPYDKESEIYYTIDGTEPSIGSKNYSAPFVTEGKIEVKAI
                     ACEPESGKCSPVAHEKFDIVRKDWKILGVDDEKAYRIIDGDPTTDWVQDKNLKMPIDL
                     VVDLGKEENLIGFKYLPSQTQWAGGVITLYKFYVSKNQKDWEMVSEGEFSNINNNPVW
                     QTKNFEKVKARFIKFQAIRNAWWNNDARYAEIDIVTP"
BASE COUNT          802 a          640 c          561 g          996 t
ORIGIN      
        1 gaccccatcc aaaacgcagc ttaccaaagc ttaaataagg aatattttct tttaccgcat
       61 tgagctcact aaatttaaac cctgccgaaa aacccgggaa gccaccaaac ctgtgatctt
      121 tcccaaaatt tgttgaacct tcgtaacgat aagtaaactg ggcaaggaac aggtttttat
      181 attcataatt taaccttcca aaataggcat attttgaacc atgtcccatt ccacccccgg
      241 cgtaggaata ttctgaacca taatccctga gggttgcatc aatgtattga tattcgggtg
      301 tggccccatt ggttagcaat gaatcagcct gaccgcttag ccctttataa aaccattccg
      361 aggcttcaaa accagccata acatcaatgg aatgttggtt gttcaaagtc attttataat
      421 cgagcacatg ttgccagttc catttaaagg ttcggtctaa ctgtctggaa acctgaatga
      481 ctttgttatt ctgagtagta ttaataaaat agtaaggctt aaaatcgtcc atttcgtaaa
      541 aattgacacc aaacccaaaa ttattagtat aggttaaacc cttgattatt tcaacattgg
      601 caaacaggtt gccattcatt gcataggttc ctttttttgc attgtaataa taaatacctg
      661 ccacagggtt accgtttccg gtgttaaaca tgggttgagc ccaacctccc caaccgatgt
      721 tcctggtttt catttgctca ggagttaagg ttgatgatgt gtctcgaaca taactgatag
      781 gactttgatt aatagaaccc atgggtgttc cccatccgtc gttaccctgg ttccgggtag
      841 cagtaagtga tatattttct ccaatacgta ttcttttgtt gatatcgaat gaattgttga
      901 ctttaattgt ataacgctta tagttagttt tatcaacaac cccgtcctga ttaaaatatc
      961 caaggcttag ataagatgca gagttttcgc tgccccccga aactgaaagg ttatattctt
     1021 ccataacagc gttgctggaa atttcgttcc accagttcgt gttgggcaac gaatctgggt
     1081 tggcagtaat ccggtttatc ggtttagtgg cgtccaatga attgtaaatt tttataaact
     1141 cttcggtatt acacatatcg acagaaatat cgttggtttg tatcccccgg tacattgaaa
     1201 aatcaacttt tgttttttgg tttttcttcc caagttttgt ggtaacgata attaccccgt
     1261 ttccacctga agacccgtaa atagcctgag ccgatgcatc tttcaaaatt tcaatatgat
     1321 cgatatcgtt ggggttaatg gtgttgatat ccccttgtat accatcgata atataaacag
     1381 gaccggctac ccctccgtac tgactttgtt ttgttattgt attaatacca cgcacacgaa
     1441 cgtatattgg agcgccgggc atccccgagc tgttgattat ctgtacacct gcagctttac
     1501 cctcaagggc ttgcaatgca ccactgctgg gagttttaga caactgctcc ccggatacag
     1561 acacgattgc cccggttaag tcgctttttt tctgtgtgcc gtatccaata acaattacgt
     1621 tgtcgatttc agatctcaat tcttttaaaa caacagtaat cgcagcagta tttccaactt
     1681 gaacttcctg catctcgtac ccgacaaatg agatcaccaa cgttgtattg tcagaaggaa
     1741 tttttacagt aaattttcca tcagagtcgg atactgtacc attagaggtt cctttaatca
     1801 ggatatttgc tcccgggatg ggaattcctg attcatcgat tattactcct tgagtaagac
     1861 gctcttgtgc ataagtgata cttaccactg aaagcaaaag aatcataata aacattcgtt
     1921 tgaaaaaatg cttacaagaa attatttccg ttatatcagt catcataatt aatattgtta
     1981 attagtgttt gttattcatt ttacgttagt ttatttcgct ttatttttgt tgatatatcg
     2041 ttaatctaca atctgtttat ggggtaacta tatcaatttc ggcataccgt gcatcgttgt
     2101 tccaccaagc atttctgatt gcctgaaatt tgatgaagcg ggctttcacc ttttcaaaat
     2161 ttttggtttg ccatacgggg ttgttgttga tattcgaaaa ttctccttca cttaccattt
     2221 cccaatcttt ttggtttttt gaaacataga acttgtaaag ggtaattact cctcctgccc
     2281 attgggtttg gctgggcaag tacttgaacc caataaggtt ttcttctttt cctaaatcga
     2341 ccacaagatc aatcggcatt tttaggtttt tgtcctgaac ccagtcagta gttgggtcgc
     2401 catcgataat ccggtaagct ttttcatcgt cgacacccag aattttccag tcttttcgaa
     2461 caatatcaaa cttttcgtga gccacagggc tacattttcc cgactctggt tcgcaggcta
     2521 ttgccttcac ttctattttt ccttcggtaa caaaaggggc tgaatagttc ttcgagccta
     2581 tacttggttc ggttccgtca atcgtgtaat aaatttcaga ctccttgtcg taaggtacta
     2641 tgataatttc gcccaactga ttccttatta ttgaaggatc cggaagaatt tgcggtgcat
     2701 tgtatacccc aatgtttgat atgagcggac aagcttttga gctagtaata ttgaaccgta
     2761 attgcgttgc ctcaactgta ggaaatcgga gtatccgctt atagcctatg gttgttcctt
     2821 ttgtgatttc tttccattca ccgccaacat ttgcttcaac ggtaaacgat tttacccgtt
     2881 gccccagacg aatgtattcc tgtgccatga aacggttaaa ggaagttggt ttctcaaagt
     2941 cgatggtcaa cgatgcagtg gtaacactgt cgtcagtagc ccaatagcta tccttgttg
//
LOCUS       DAOPUW010000186         2993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_34038_length_2993_cov_3.179033, whole genome shotgun sequence.
ACCESSION   DAOPUW010000186 DAOPUW010000000
VERSION     DAOPUW010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34038_length_2993_cov_3.179033"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2630)
                     /locus_tag="PKJ43_04135"
     CDS             complement(<1..2630)
                     /locus_tag="PKJ43_04135"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNZ71784.1"
                     /translation="MKSVKSLNIFVGIATFVVSFIIYLFTLEPSVSFWDCGEFIAASY
                     KLQVGHPPGAPLFMIIARVVSIFAGNPTNVAFMVNALSALASAATVMFLYWTIVRLAK
                     KIHGENESHFSEYVRIGAGIIGAMTFAFTDSFWFSAVEAEVYALSSLFTAVVFWAILR
                     WEEVAEQEHSNRWIILIAYLIGLSIGVHLLNLLAIPAIALIWYFKKYTPTTKGIIATL
                     SVSALIIAFIMWGIIQGTPTIAQKFEVLFVNSFGLPYNSGLVFFILLIVAVAIGLIRY
                     SHKHNKPMLNLSMVAFVLIMMGYFSYAVVMIRSKADPPMDENNPENAYTLMKYLNRAQ
                     YGTTPLLTGYYFNAPAVAVEKGDPVYYPKNGKYVKIEGSPNYKYDDRFKTFFPRMYSS
                     QSTNHESGYKMWGEIKGKPVTINGEKEIIPTFSENLRFFFSYQLNHMYFRYFMWNFSG
                     RQNDNQGHGDVLNGNWLTGIGFIDKIRLGHNGSQPAPMANAETTNRYFMLPFLLGLLG
                     MFFHYRRHKKDFWVVMLLFFMTGIAIVMYLNQPPYQPRERDYAYTGSFYAFAIWIGIG
                     VLALYELLQKRSKLAMPIALGVTSIVPILVLVQNFDDHDRSQRYVARDMGKNYLNSCE
                     KNAILFTYGDNDTFPLWYVQDVENARPDVRICNVTLLNGDWYIDQMKRKVYSSDPLPI
                     SMDNSKYENEVRNSVFVRDDIKRPVDISQALDLVLSDDPRTKLRTQSGTLYSYLPSKQ
                     LKITVNKQKVLSTGTVSADKASQIEDSIIINIPGSYISKSDLAILDMLAHNDWERPIY
                     FDLSVVNTSGLKLEKYLQNEGFAYRLVPLRNNSGEPMINSDILYNRLMNQFTWGNLNK
                     NILVDETLRNTIDVIQLKNN"
BASE COUNT          864 a          617 c          644 g          868 t
ORIGIN      
        1 aaattgtttt tcaattgaat aacatcgatc gtgtttctca acgtttcatc gaccaggata
       61 tttttgttca aatttcccca agtaaactga ttcatcaacc ggttgtataa aatgtcggaa
      121 ttgatcatcg gttctccgct attgtttcgc agaggcacca aacgataggc aaatccttcg
      181 ttttggaggt acttttcaag tttaagcccc gatgtgttca ccacgcttaa atcgaagtaa
      241 attgggcgct cccagtcgtt gtgggcaagc atatcgagta tggcaaggtc gcttttcgaa
      301 atatacgagc ctggaatgtt tatgatgatt gaatcttcaa tctgtgaagc tttatcggca
      361 gaaacagttc ctgttgaaag gactttttgc ttgtttactg ttattttcag ctgttttgat
      421 ggaagatagc tgtatagtgt accgctttgc gtccgaagtt ttgttcgtgg gtcgtcgcta
      481 aggactaagt ctaaggcttg cgaaatgtca accggccttt taatgtcgtc gcgtacaaaa
      541 acagagtttc gtacctcatt ttcatatttc gaattgtcca tcgaaatggg caacgggtct
      601 gagctgtaca ctttcctttt catctgatcg atataccagt cgccgtttaa cagggtaaca
      661 ttgcagatcc ttacgtcggg acgggcattc tcaacatcct gaacatacca aagcggaaac
      721 gtgtcattat cgccataggt gaaaagtatg gcattttttt cgcacgagtt aaggtagttt
      781 ttccccatgt cgcgggctac atatcgttgc gacctgtcgt ggtcgtcgaa attttgaaca
      841 agcaccaaaa taggaactat ggaggttaca ccaagagcga ttggcatggc aagtttcgac
      901 cgtttctgca aaagctcgta caaggccagc actccaattc caatccatat tgcaaaagca
      961 taaaacgatc cggtataggc ataatcgcgt tcccggggtt gatacggggg ttgattcaaa
     1021 tacatgacaa tcgcaatacc tgtcatgaag aaaagcagca taaccaccca gaagtctttt
     1081 ttatgcctcc ggtagtggaa aaacattcca agcagtccca gcagaaaggg cagcataaaa
     1141 tagcggtttg ttgtttcagc attggccata ggtgcaggtt gactgccatt atgccctaat
     1201 cgtattttat caataaaccc aattccggta agccagttgc cgttgagtac gtcgccatga
     1261 ccctggttgt cgttctgccg gccggaaaag ttccacatga aatatctgaa atacatgtgg
     1321 ttcagctggt atgaaaagaa aaaccgtaag ttttcactaa atgttggaat gatctctttc
     1381 tctccattta tcgttactgg ttttcctttt atttcacccc acattttata cccgctttcg
     1441 tggtttgtcg actgcgagct gtacattctt gggaagaatg ttttaaaacg gtcgtcgtat
     1501 ttataatttg gcgaaccttc tatttttacg tatttgccgt ttttggggta atacacaggg
     1561 tctccctttt caacggcaac agcgggagca ttgaaatagt atcctgtaag taatggagtt
     1621 gttccatatt gtgcccggtt gaggtatttc attaatgtat aggcattttc gggattattt
     1681 tcgtccattg gaggatcggc tttcgagcgg atcataacca ccgcatacga aaaataaccc
     1741 atcatgatca acacaaatgc taccattgag agatttagca taggtttatt atgcttgtgc
     1801 gaatacctga ttaatcctat cgcaacagct actatcagaa gaatgaaaaa gacaagtccc
     1861 gaattgtatg gaaggccaaa ggaattgaca aacagcactt caaatttttg ggcgatggtt
     1921 ggcgttccct ggattattcc ccacatgata aaggcaatga tcagggccga aacagaaaga
     1981 gtggcaatga tgccttttgt agttggagta tatttcttga aataccagat taaagcaata
     2041 gccggaattg caagcaggtt tagcaggtgc actccaattg aaagtccaat taagtatgca
     2101 ataaggataa tccaacggtt tgagtgctct tgctcggcaa cttcttccca gcggagaatg
     2161 gcccaaaaaa ctaccgccgt aaataacgaa gacaaggcat aaacctcagc ctcgacagcc
     2221 gaaaaccaaa acgaatcggt aaatgcaaaa gtcattgcac caataatacc agcaccaatt
     2281 cttacatatt cagaaaaatg tgactcattt tcgccatgaa ttttttttgc aagccggacg
     2341 atagtccagt aaaggaacat aactgttgct gcactggcca gggctgagag ggcatttacc
     2401 atgaaagcca cgtttgtggg atttcctgca aatatcgata ctacgcgggc aataatcata
     2461 aataaaggtg ccccgggtgg atgaccaact tgcagtttgt acgaggctgc aataaactcg
     2521 ccgcaatccc aaaagctaac cgagggctca agggtgaaca ggtaaatgat gaaggaaaca
     2581 acaaatgttg caattcctac aaaaatgttg agcgatttaa ctgatttcat ggattcttgt
     2641 aaaagatttc agcaaatata gtttgattat gattaaaatg attctcattt atgatttgtt
     2701 aagaaatgag caggtgtaaa agcatccatt ttctgcaact ttccatttat tgcattcttg
     2761 cattatcttt gccctccaat tataacgttt tgaggcaaac acctttcgtg acctttgtga
     2821 acatcctttg agtcctttgt gtttatgcct cgtccttaaa gaatgaaaat gaagaaagat
     2881 attcgcaaag agctggaaaa gcgtgtgttg gtacttgatg gagccatggg ttcgctcatt
     2941 caaacctata aactcaccga ggccgattac cgtggcgagc aattcaaaaa cgc
//
LOCUS       DAOPUW010000187         2985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_34176_length_2985_cov_4.043345, whole genome shotgun sequence.
ACCESSION   DAOPUW010000187 DAOPUW010000000
VERSION     DAOPUW010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34176_length_2985_cov_4.043345"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PKJ43_04140"
     CDS             <1..787
                     /locus_tag="PKJ43_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNZ71785.1"
                     /translation="GNLSGMLYAIDLNTGKKKWEYKTDNQIMGAPSFAEAGGKKIIVA
                     GSYDFFLHGVDAQTGKGLWKYESDNYLNSAPAIHNQKAIFGGCDGFLHQVLLTSGKAA
                     SKTQIATYVASSPAIDGNKAFVGDYDGGFSCIDLSSSKVVWKFENKESNLPFIASASV
                     INDRVIIGSRDKNIYCFNKANGKIQWKTNTGVRVDASTVVNSKEVLAVNMRGDVMLLD
                     IQTGKKIWNYELGTGVMNTPAVIDGAIVLAGSDGNLYLLAASK"
     gene            815..2104
                     /locus_tag="PKJ43_04145"
     CDS             815..2104
                     /locus_tag="PKJ43_04145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNZ71786.1"
                     /translation="MNIIQIIPGSGGSFYCGNCLRDSKFVDSLKKQGHSVIKVPMYLP
                     LFSDERDLTDIPVFYGAISIYLKQLYPIFRKAPAWFDRLLNSKPLMKLAAGMAGSTNA
                     KGLEEMTVSMLLGEEGEQRKELDKMADWIAEHCKPDVIHLSNALLLGLARRMKQKLNV
                     PVVCSLQDEDIWVDVMKPSMREKVWKLMEEKATDVDAFIPVSGYYAQVAKQRMNIPDE
                     KMHTIHLGVDPSDYSFSNASERKREIGYLSRMNAENGLDILVDAFILLKKEERFSDVH
                     LHITGGWTGDDIHYIKEQKAKLVSAGIRSFAHFWEGFEIEHRTHFLKRVQLMSVPVRN
                     GEAFGIYLSEAMASGIPVVQPNLGAFPEIVNTAGGGIIYEPNTPEALCKALQNLLDDQ
                     PLINKLSIEARKSVEEHFDINTQAEKMIAVYQKYVNR"
     gene            2282..>2985
                     /locus_tag="PKJ43_04150"
     CDS             2282..>2985
                     /locus_tag="PKJ43_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71787.1"
                     /translation="MHLKALIFCAFFCSATSLFGQDLSQISKQKPVNVDGSIAATSNF
                     YSINGKESGRDPFSYILSGNVNVSIYGLQLPFSFMYSNSSFSYAQPLNRFGISPEYKW
                     IKLHIGYRSITFSPLTLAGHSFLGAGIELTPGKFRFGAAYGRFNKKTIPNTNNPLDTL
                     QTPTRKGFSMKIGVGTPKNYFDLIMLKIADDTLSFDKTKYEPKIPQSNTVLGTHMKFT
                     LFKNLTWETEGAISLLT"
BASE COUNT          960 a          627 c          639 g          759 t
ORIGIN      
        1 ggggaatctt tcgggaatgc tttatgccat cgacctgaat accggtaaaa agaaatggga
       61 atataaaaca gacaaccaga ttatgggagc cccttctttt gccgaagccg ggggtaaaaa
      121 aattattgtg gctggcagtt acgatttttt ccttcacgga gttgatgccc aaacagggaa
      181 aggactttgg aaatacgaat cggacaatta tttgaacagt gccccggcaa ttcataacca
      241 gaaagctatt tttggcgggt gcgacggatt tctccatcaa gtcctcctta caagcgggaa
      301 ggctgccagt aaaacacaaa ttgccacata cgttgcttca tcgccagcaa tagacgggaa
      361 caaagccttt gtgggagact atgatggagg attttcgtgc atcgacttat caagctcgaa
      421 agttgtatgg aaattcgaaa acaaggaatc gaacctaccg tttatcgctt ctgcatcagt
      481 aatcaacgac cgagtaatta ttggaagccg cgacaaaaac atctactgtt tcaacaaggc
      541 taacgggaaa atacaatgga aaactaacac cggtgtcagg gttgatgcat ctacagtggt
      601 caatagtaaa gaagtgttgg cagtgaacat gcgaggcgac gttatgctac tcgacataca
      661 aacagggaaa aaaatatgga actacgaatt gggcacggga gtaatgaaca ctccggcagt
      721 tatcgacggc gcaattgtgc ttgccggttc cgacggaaac ctataccttt tggcagcttc
      781 gaaataacaa acaaataatc agcaaatagc aacaatgaat attatacaaa taatacccgg
      841 atcaggagga agtttttact gtggcaattg cttacgcgac agcaaatttg tcgattcttt
      901 gaaaaaacaa ggccactcgg taataaaagt ccccatgtac cttcccctct tttcagacga
      961 acgcgatctg acagatatcc ctgtatttta tggtgccata agtatttacc tcaaacagct
     1021 ttaccctatt ttcaggaaag cccctgcttg gttcgaccgt ttgctcaatt cgaaaccgct
     1081 aatgaaactg gctgcaggta tggctgggtc gaccaacgca aaaggtttgg aagaaatgac
     1141 cgtttccatg ttgcttggcg aagaaggcga acagcgcaaa gaactggata aaatggccga
     1201 ttggattgcc gaacattgta aacccgatgt aattcacctt tcgaatgcct tgttgcttgg
     1261 gctggcccga agaatgaaac aaaagctgaa tgtcccggtg gtgtgctcgc tgcaagatga
     1321 agatatttgg gttgatgtaa tgaaacctag catgagagag aaagtgtgga aacttatgga
     1381 ggaaaaagca acggatgttg atgcttttat ccccgtcagt ggatattatg cacaagtagc
     1441 aaaacagcga atgaacattc ccgatgaaaa aatgcataca atacatcttg gggtagatcc
     1501 ttccgattac tcattttcga atgcatcaga aagaaaacgc gagattggat acctttcacg
     1561 catgaatgcc gaaaatggac tcgatatttt ggtcgatgcg tttatcctct taaaaaagga
     1621 agaacgtttt tccgacgtgc atcttcatat aaccggtggt tggaccggag acgacattca
     1681 ctacatcaaa gaacaaaaag caaaactcgt aagcgctggc ataagatcat ttgcccattt
     1741 ctgggaagga tttgaaatag agcaccgaac ccattttttg aaaagggtcc agctaatgtc
     1801 tgtgcctgtt cgaaatggcg aagcctttgg catttacctt tctgaagcca tggcttcggg
     1861 cataccagta gtacaaccca acttgggagc ctttcccgag attgtaaata ctgcaggtgg
     1921 tggaataatt tatgagccca acacacctga ggcactgtgc aaagcactgc aaaaccttct
     1981 cgatgaccag ccactgatta acaagcttag cattgaggcc agaaaaagtg ttgaggaaca
     2041 tttcgacata aacacacagg cagaaaaaat gattgccgta tatcagaaat atgtcaacag
     2101 ataggaaaat gcgataagga ttgaagacaa attcatgtaa aagaacaaca tatttaagct
     2161 agattacatc gaattcaaag aaagtactgg tttcaaaata attatactgg caaaataacg
     2221 ttgcttcaat tttttttgta catttgatag actcattaat gggatattaa aagagtacac
     2281 catgcatttg aaagccctta ttttttgcgc ctttttctgt agtgctacct cgctgtttgg
     2341 tcaagacctc tcacaaatat cgaaacaaaa accggttaac gttgatggga gcattgctgc
     2401 aaccagtaat ttttattcaa tcaacggcaa agagtcggga cgcgatccat tttcgtatat
     2461 tttatctggt aatgtaaatg tttcgatata cgggcttcag ttgccctttt cattcatgta
     2521 cagcaacagc agttttagct atgcacaacc cttaaaccga tttggcataa gtcccgaata
     2581 taaatggata aaacttcaca taggctaccg aagtataacg ttctcaccgc taacccttgc
     2641 cggccattca tttcttggtg ccggtattga attaaccccc ggaaaattca ggtttggagc
     2701 cgcctatggc cgtttcaaca aaaaaaccat acccaacacc aacaacccgc tcgacactct
     2761 gcaaacccca acgagaaaag gattcagcat gaagattggt gttggtactc caaaaaatta
     2821 cttcgacctg attatgctta agatcgccga tgacacctta tcgttcgata aaacaaaata
     2881 tgagccgaaa atacctcaat caaatacggt tctgggtaca cacatgaaat ttaccctatt
     2941 caaaaaccta acgtgggaaa ccgaaggagc aataagcctg ttaac
//
LOCUS       DAOPUW010000188         2983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_34208_length_2983_cov_3.313866, whole genome shotgun sequence.
ACCESSION   DAOPUW010000188 DAOPUW010000000
VERSION     DAOPUW010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34208_length_2983_cov_3.313866"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            553..807
                     /locus_tag="PKJ43_04155"
     CDS             553..807
                     /locus_tag="PKJ43_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNZ71788.1"
                     /translation="MNIYVGNLDFKVNGDDLQGIFEEYGTVSSTKIIVDKFSGRSKGF
                     GFVTMDNQNEAQNAVNELNGATLENRKMVVNEAKPKKENY"
     gene            820..1020
                     /locus_tag="PKJ43_04160"
     CDS             820..1020
                     /locus_tag="PKJ43_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010423634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNZ71789.1"
                     /translation="MAKGKVKWYNEVKGFGFIASETGEDLFVHRTGLASPYSRLQPDQ
                     EVVFETKEGEKGLMAVGVKPAN"
     gene            1115..1579
                     /locus_tag="PKJ43_04165"
     CDS             1115..1579
                     /locus_tag="PKJ43_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNZ71790.1"
                     /translation="MGRSQESFNKKEVRKQKEKKRKEKTDKKLARKETGKTSFDDMIA
                     YVDEFGQITATPPDPDKKIDIDVEDIEIGISKADPSLKADPTRKGIVTFFNSSKGYGF
                     ITDMETRQSVFVHVNNLLESIKENNVVNYEVVKGVKGPTAIKVKLFRKEQPQ"
     assembly_gap    1839..1848
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1851..>2983
                     /gene="gdhA"
                     /locus_tag="PKJ43_04170"
     CDS             1851..>2983
                     /gene="gdhA"
                     /locus_tag="PKJ43_04170"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNZ71791.1"
                     /translation="MARTAGEKEFHQAVQEVVESVWDIYDSNPRFKKAKILERMTEPE
                     RVIMFRVPWVDDRGDVQINRGYRVEFNSAIGPYKGGLRFHPTVSLSVLKFLGFEQTFK
                     NSLTTLPMGGGKGGSDFNPKGKSDNEIMHFCQSFMTELYRHIGPNTDVPAGDIGVGGR
                     EIGYLFGQYKRLKNEFTGVLTGKGIHWGGSLIRPEATGFGSVYFIRHMLKEMGKDIEG
                     HTFSVSGFGNVAWGVVSKLNELGGKVLTISGPDGYIYDKDGISGDKVNYMLELRASNN
                     DVVQPYADEFGAKFIPGRRPWELPTDIAIPCACENELNLDDAKELVKNGCFIVAETSN
                     MGCNSEAAEYLNENITFAPGKAVNAGGVAVSGLEMSQNSMRYN"
BASE COUNT          981 a          526 c          629 g          837 t
ORIGIN      
        1 cgagggcgaa cgtttctacg atctgatgcg tattgctaag cgccgaaacg acccgtcgta
       61 cttagcttca cgtgtgtctg caaaatatcc cgcaagccgc aggcaggcta tttataatta
      121 tctgcttaat gaggacaatt ggtatataca ctactttcaa taatattttt gtttaatagg
      181 ggagattaga ttgaatgaag ggattgccgg ctattatgtc aggcagtctc ttttttcgtt
      241 ttacaccctt catgtcaaac aattattgcc aaaaagaatt gattaaattg gaatacttta
      301 ccgaacgatg gttcacatta aaaaaaaact atctttgtaa aaatttcgat ttaaaaattt
      361 gagcacttat ctactaaacc catcaaagtt ttagttaatt actgcactac ttcttgaatc
      421 ctgattttta agcccgatat ttatgacagg gtttatgctg ctgaattaga aggaattaca
      481 atgtaatgcg ttaatattta gtatgttttc tctgtcggtg tggggtagca taaacaatta
      541 aaagaaaaaa aaatgaacat ttacgttgga aaccttgatt tcaaggttaa tggagacgac
      601 cttcagggaa tcttcgaaga gtacgggaca gtaagctcta caaaaattat tgtcgacaaa
      661 tttagtggca gaagcaaagg ctttggtttt gtaactatgg ataatcaaaa tgaagcccaa
      721 aacgctgtaa atgaattaaa tggtgcaact ctcgaaaacc gtaaaatggt tgtaaacgaa
      781 gcaaaaccta aaaaagaaaa ctactaatac tttaatcaaa tggcaaaagg taaagtaaaa
      841 tggtataatg aagtgaaagg ttttggcttc attgcctccg aaacaggcga agatttattc
      901 gttcaccgca ctggtcttgc atctccatat agcagattac agcccgatca ggaagttgtt
      961 ttcgaaacaa aagaaggcga aaaaggatta atggctgttg gtgtaaaacc tgccaattaa
     1021 tcatatcata ttatttgacc caagtggaac agcaagccct tgttgtccgt tgggtcaaat
     1081 tttttttaat acagcaaatc gtaaataagt aaaaatgggt agatcacaag aatcatttaa
     1141 caaaaaagaa gttagaaaac aaaaggaaaa aaagagaaaa gagaaaaccg acaaaaaatt
     1201 agctcgtaaa gaaactggga aaaccagctt cgacgacatg attgcttacg tcgacgagtt
     1261 cggtcaaatt acggccactc ctcccgatcc cgacaaaaaa attgatattg atgttgaaga
     1321 tattgaaatc ggtatctcaa aagccgaccc ttcgctaaaa gctgatccaa cacgcaaggg
     1381 aattgtaaca ttctttaata gctcaaaagg ttatggtttt attaccgaca tggaaacccg
     1441 gcaaagtgtt ttcgtgcatg ttaacaatct tctcgaatct ataaaggaaa acaatgtcgt
     1501 taattacgaa gtggtaaagg gagttaaagg gccaacagct ataaaagtaa agcttttcag
     1561 aaaagaacag cctcagtagt ttcttcccat ataatcattt tctttatttg gtatataggt
     1621 cagtattcag gatagttttt cccgaatctt ggttcatgat aaaaatcagg tgtacttggc
     1681 aagaaagtgt taataaatgt taaaacactg tttctctgcc agctacatct ttactttttt
     1741 gtagggcaaa atgcttttgc cgacgggttt gtcataactt taaataaaac aaaatgaaaa
     1801 gttatgagac ccgaaatcaa tgaattcatg agccacttnn nnnnnnnnta attgcccgca
     1861 cagcaggcga aaaagagttt catcaggcag tacaggaagt agtcgaatct gtttgggaca
     1921 tctacgatag caacccacga ttcaaaaagg caaaaatact tgaacgaatg actgaacctg
     1981 agagggttat aatgtttcgt gtcccttggg ttgacgatcg cggagatgtg caaataaaca
     2041 ggggctatcg tgtagaattt aatagtgcca ttggccctta taaaggaggt cttcgttttc
     2101 acccaaccgt ttcacttagc gtgctgaagt ttctcgggtt cgagcaaact ttcaaaaaca
     2161 gtcttaccac attacccatg gggggcggaa aaggaggatc tgattttaat cccaaaggaa
     2221 aatcggacaa cgaaataatg cacttttgtc agagcttcat gactgaactc tacaggcaca
     2281 ttggtccaaa caccgatgta cctgccggtg atattggtgt aggcgggcgt gaaattggtt
     2341 atctattcgg ccaatataaa cggctcaaaa atgaattcac cggagtactt acaggaaaag
     2401 gaatccattg gggcggtagc ctcatccgtc ccgaagcaac aggtttcggt tctgtatact
     2461 tcattaggca tatgttgaaa gaaatgggta aagacatcga aggccataca ttctcggttt
     2521 ccggattcgg aaatgttgct tggggggtag tctctaaatt aaacgagtta ggtgggaaag
     2581 tactaactat ttcaggtccc gatggttata tttatgataa agatgggatt agcggcgata
     2641 aagtaaatta catgctcgaa ttgcgtgctt caaataacga tgtggttcag ccgtatgccg
     2701 atgaatttgg cgcaaaattt attccaggca gaaggccttg ggaattgcca actgatattg
     2761 caattccttg tgcttgcgaa aacgaactta atctcgacga tgcaaaagaa ttggttaaaa
     2821 atggctgttt cattgttgcc gaaacatcta acatgggctg taattccgaa gcggctgaat
     2881 atcttaacga gaatattacg tttgcaccgg gtaaggctgt caatgctggc ggagttgctg
     2941 tttctggcct cgaaatgtca caaaattcta tgcgttataa ctg
//
LOCUS       DAOPUW010000189         2979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_34278_length_2979_cov_2.569083, whole genome shotgun sequence.
ACCESSION   DAOPUW010000189 DAOPUW010000000
VERSION     DAOPUW010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34278_length_2979_cov_2.569083"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..1027)
                     /locus_tag="PKJ43_04175"
     CDS             complement(200..1027)
                     /locus_tag="PKJ43_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNZ71792.1"
                     /translation="MVKGEVFYLDNTLCSADQWNEGLETGTVVYEVLRIVDGVPLFYL
                     DHYQRLMNSCQLVGKKGQLDKDQLFTNMVTLAAENNLLIGNVMLKVIFSQSAGFPYET
                     PRTLLYFIPHSYPSDQQYITGVDVGILKAERQNPEAKVEQGVRNIANKILTESTLFEV
                     LLVDREGYITEGSKSNMVFVKNEVLYTCPLSKVLTGITLLKVIEIAEKENISLKFTAV
                     PLKQLHEFDALFITGTSPKILPVRRVGEIQFDVNNQLMRFLMEKYNYLLQFDISKRS"
     gene            complement(1079..2242)
                     /locus_tag="PKJ43_04180"
     CDS             complement(1079..2242)
                     /locus_tag="PKJ43_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71793.1"
                     /translation="MKSHVCSLVIFGLFFYLTISCSAKDEHNPANDSMIIDHHCTNIF
                     AVPVEYIQAAKSKLVIAYGHTSHGSQIISGMGSLDDFMQKHDYQPGLFNYNSDGENGA
                     LELHDSPFEEAADLGNPDRTSWASTTRKYLENHSDINVIMWSWCGQAAWASNEEIDLY
                     LSLINDLETEFPSVRFVYMTGHLDGTGAEGQLNQNNNRIREFCRSNKKVLFDFADIES
                     YDPDGKVNYMVLNANDNCDYQTSTGESRNWAIDWQNSHTENVDWYDCGAAHSQSLNGN
                     RKGYAAWWMFARLAGWDTAPDTTVSSSQFSKDQFTYKVLGDEIVFSSSDRKTIDGIVF
                     YDVTGRVLYKNNTKTNGELFVPRSVFGNTPVVLYRITSDRKSFSGKILLQKAN"
     gene            2627..>2979
                     /locus_tag="PKJ43_04185"
     CDS             2627..>2979
                     /locus_tag="PKJ43_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71794.1"
                     /translation="MKKVTLFGVIILVIALTVFVYIRYFFVFGEGVKAGDLNFIVYKG
                     MVFKTWEGRVIQTGIRTNTPGAIQSNEFEFSVKNDSIAKVLEVNSGRFVELRYKEYFH
                     SLPWRGNSKYVVHEI"
BASE COUNT          926 a          602 c          550 g          901 t
ORIGIN      
        1 atccaaaggt tggtaccttt gaaaacaatt cctttttcta tttcactgtg aataaaatcg
       61 taatcctcaa tttcactgcg aaggtcgagg taatggaata ttttttctaa aaaattcata
      121 acatgctttt ttagttcatt atcctaagat actgattttt tctaaccttt catacgttga
      181 atttctgaaa gtaaaaacat caggagcgtt tcgagatatc gaattgaaga aggtaattgt
      241 atttctccat caaaaaacgc atcaattggt tgttaacatc aaactggatc tctcctaccc
      301 ttctgaccgg tagaatcttt ggtgaagttc cggtgatgaa taatgcatcg aactcgtgaa
      361 gctgtttcag gggtacggct gtaaacttta atgaaatatt ctctttttca gcaatttcaa
      421 ttactttcag caatgttatt ccagtcagta ctttgctcaa cggacaagta tagagcactt
      481 catttttaac aaaaaccatg ttcgatttgc ttccttcggt gatatagccc tcacgatcga
      541 ccagcagtac ttcgaaaaga gtgctttctg taagtatctt gttcgcaata ttccttaccc
      601 cctgttcaac tttagcttca ggattttggc gttctgcctt gagaattcca acatcaacac
      661 ctgtaatata ctgctgatcc gaaggataac tatgaggaat gaaatacagt aaagtccgtg
      721 gggtttcata cgggaacccg gcagattgag aaaaaataac cttaagcatt acatttccga
      781 tgaggagatt attctctgct gccagagtca ccatattggt aaacaactga tccttgtcaa
      841 gttgtccttt tttcccaacc agctggcacg agttcattaa ccgttgataa tggtcaagat
      901 agaaaagcgg aacgccatct acaattctca agacttcata tacaacggtt cccgtttcaa
      961 gcccttcgtt ccattgatct gcagaacaaa gggtattatc taaataaaaa acttctcctt
     1021 ttaccatgtc gaaccatttt catgcaaaaa taatcgcgaa atggcggaaa tgtgcatgtc
     1081 aattggcttt ctgcaacaaa atcttccctg agaagctttt ccggtctgac gtaatacggt
     1141 acaaaacgac aggagtgttt ccaaagacag accgtggtac aaacaactca ccgtttgttt
     1201 ttgtgttgtt tttgtaaaga actctcccgg taacatcata aaatactatt ccgtcaatcg
     1261 ttttcctatc ggaggatgag aaaactattt cgtcgcccaa aactttgtat gtaaactgat
     1321 ctttagaaaa ttgacttgat gaaaccgtcg tatcaggggc tgtatcccaa cctgccaggc
     1381 gggcaaacat ccaccatgca gcatatcctt tccggttccc gtttaacgac tggctgtgcg
     1441 cggcaccaca atcataccaa tcgacgtttt cagtatgtga attttgccaa tcgatagccc
     1501 aattcctcga ctcaccagta ctggtttgat aatcgcaatt atcgttggca ttcaaaacca
     1561 tatagttaac ttttccgtcg ggatcataac tttcaatatc agcaaaatcg aaaagtactt
     1621 tcttgttgct gcgacagaat tcccgaatcc ggttattatt ctgatttagt tgcccttcgg
     1681 ctccagtacc atcgagatgg cctgtcatat aaacaaaacg taccgaagga aattctgttt
     1741 ccagatcgtt tatcagcgac aggtaaagat ctatttcctc gttcgaagcc catgctgctt
     1801 gcccgcacca cgaccacatg attacattta tatcggaatg attttccaaa tacttacgag
     1861 ttgtcgatgc ccacgaagtg cgatcaggat ttcccaaatc ggcagcctct tcaaagggag
     1921 aatcgtgtaa ttcgagagct ccgttttcgc cgtctgaatt gtaattaaat agccctggct
     1981 gataatcatg tttttgcata aaatcatcga gagagcccat gcccgatata atctgacttc
     2041 catgcgaagt atgcccgtat gcaattacca acttcgattt agcagcttga atatattcaa
     2101 caggaacagc aaaaatattg gtacaatgat ggtcgattat cattgagtca tttgctggat
     2161 tatgctcatc tttagcagaa cagctaattg taagatagaa aaacaatcca aaaataacta
     2221 aagaacacac atgcgatttc atagtattgt aggtttagag gtttacttga gcaatttgaa
     2281 aatcaatatt gtaataacga tcaaaagtaa gactatagca acataaaaac aacgctctcc
     2341 attaaaaacg aagagcgttg taaaattatt atatgtttag cctatcgggc tggcggatat
     2401 gcgcccatta tggtctaatt ttaaataaaa ggcttaatga tttttattaa tatcgaaaca
     2461 aaaacgttac aaatagttga tatttaccct actgcaattc cctgtgcaaa tcaagaatac
     2521 agaatgaaat tttaccccct ctggtaagat tgaatgagga aaaaaagcga tatttgcttg
     2581 acttcgggat attccctaca tttataaaaa attaattaag aaaaagatga aaaaggttac
     2641 actcttcggc gttattattc ttgtaattgc actgacagtt tttgtttata tccggtactt
     2701 ttttgtattc ggagaaggtg taaaagctgg cgatctcaat ttcatcgttt acaaagggat
     2761 ggtttttaaa acctgggaag gccgggtaat acaaaccggt ataagaacca atacgcccgg
     2821 tgctatccaa agcaacgaat ttgaattcag tgtaaaaaac gacagcattg ccaaagtgtt
     2881 ggaagtgaat agcggtagat ttgtagagct tcgctacaag gaatattttc actcgttacc
     2941 atggagggga aacagcaaat atgttgtgca tgaaataat
//
LOCUS       DAOPUW010000190         2975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_34340_length_2975_cov_3.880137, whole genome shotgun sequence.
ACCESSION   DAOPUW010000190 DAOPUW010000000
VERSION     DAOPUW010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34340_length_2975_cov_3.880137"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="PKJ43_04190"
     CDS             complement(<1..1153)
                     /locus_tag="PKJ43_04190"
                     /inference="COORDINATES: protein motif:HMM:NF028046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71795.1"
                     /translation="MTLQRNKTFQNRIFFSFFAAFALLATAVLTFQYQREKEYRVKQL
                     ENTLDNITVIVHNYIDQNNLLETKNFNGINTLVDLLPSEDERITIIDSKGNVLFDSFV
                     DNIASMENHLERPEIQKAFFSGVGKNIRKSATTNTEFYYYARYYNNYFVRTALVYDVQ
                     VKNYLHIAQTFIIFIIVLFALMWLIISIVTKKVSSFVTQLKDFSVKAAIGEEIENEKT
                     FSDNEFETISQQIKKIYNQLNGAKAALSKERDKLFQHLNVLHEGIAFFHPNRERFLTN
                     SHFIHYVNIISEKSTVSAPNVLEIPELKPLVDFINQYQNEAFSSAPDLPSMCITVEKN
                     EFVFDIKTIIFSDKSFEILITDTTRLEKRKQIKQQLTANIAHELKTPVAS"
     gene            complement(1236..1913)
                     /locus_tag="PKJ43_04195"
     CDS             complement(1236..1913)
                     /locus_tag="PKJ43_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ71796.1"
                     /translation="MIEDDIKILVVDDEKDLCEILQFNLESEGYEVDTANSAEEALKK
                     NLHEYDLILLDVMMGGMSGFKAADTMRKQKQIEIPIIFITAKSSENDLLTGFNVGGDD
                     YIQKPFSVKEVIARVKAVLKRGKHTSTEKISCDTLEIDLSRKLAYIDGRIIDLTRKEF
                     DILLLLMKKKGSYVSREEILDRVWDDDVVVTERNVDVNIARLRKKIGEYGNHIKGKTG
                     YGYLFEE"
     gene            complement(1947..2654)
                     /locus_tag="PKJ43_04200"
     CDS             complement(1947..2654)
                     /locus_tag="PKJ43_04200"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HNZ71797.1"
                     /translation="MSQKKEEAIQKVMTEFESMANLVLSQLDRLESFFQSGETNLPEK
                     LREEFDANEKLIDQYEVKLSERIINTIVLFQPVASDIRQLIACYRIIINLERIGDYTI
                     NIVKFFSEIKNLELYEQMIGAINPMLITSTQMVTKSLLSFIHHDKEYAIWTIKNDDMV
                     DDMNRKLLKGMVSRSKSFDDKKKTLISFITIKDMMDNIERMADHATNIAEAAIYYMEG
                     KDIRHHPLDDEMMNINQ"
     gene            complement(2676..>2975)
                     /locus_tag="PKJ43_04205"
     CDS             complement(2676..>2975)
                     /locus_tag="PKJ43_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HNZ71798.1"
                     /translation="QQRLCIARALAFNPEIILLDEPTSALDPIATSKIEDLVVKLKEN
                     YTIIMVTHNMSQAARISDYSLFMYLGELVEYNKTKIMFTNPKDRRTEEYLTGKFG"
BASE COUNT          733 a          565 c          616 g         1061 t
ORIGIN      
        1 gcgatgcaac cggcgttttt agttcatgtg caatatttgc ggtaagctgt tgttttatct
       61 gttttcgttt ttcgagtcgt gtggtatctg taatcaatat ctcgaagctt ttgtcggaga
      121 agattatggt ttttatgtcg aatacaaatt catttttttc aacggtgata cacatgcttg
      181 gcaggtcggg tgccgacgaa aacgcttcat tctggtattg gtttatgaaa tcgacaagag
      241 gttttaattc cggtatttca agaacgtttg gagcagatac agttgatttt tctgaaataa
      301 tattcacata atgaataaaa tgactattgg ttaggaagcg ttctctgttg ggatggaaaa
      361 aggcaatgcc ctcgtgcaac acgttgaggt gctggaacaa tttatcgcgc tctttcgata
      421 gtgctgcttt tgccccatta agttggttgt aaattttttt gatttgctgg cttattgttt
      481 caaactcgtt gtcggagaat gttttttcgt tttctatttc ttcgccaata gccgctttta
      541 ccgaaaaatc tttcaattgg gtaacaaagc tgctaacttt tttggtaaca atgcttatta
      601 ttagccacat taatgcaaac agaacgataa taaatatgat gaatgtttgg gcaatatgga
      661 gataattttt cacctgtaca tcgtatacta aagcagtgcg tacaaagtag ttgttgtagt
      721 agcgggcata ataatagaac tctgtattgg tagtggccga tttacgaata tttttgccta
      781 ctcctgaaaa aaaggctttt tgtatttcag gcctttcaag gtggttctcc atcgaggcaa
      841 tgttgtcgac aaaactgtcg aaaagtacat tgcctttcga atcgattatg gtgattcgct
      901 catcttcact ggggagaaga tcaacgagtg tgtttatacc attaaaattt tttgtttcga
      961 gaaggttgtt ctgatcgata taattatgga caatgaccgt gatgttgtcg agagtattct
     1021 cgagctgctt aacccggtac tctttttctc gctgatattg aaaggtgagc acggctgttg
     1081 caagcagtgc aaaggctgca aaaaacgaga aaaaaatcct gttttgaaat gttttgttcc
     1141 gttgcaatgt catagtcttc atttttatac cggaaattga aatgttgctc gcttcccgtt
     1201 ttgttattta ttttttgtac ccagggttgg catgcttact cttcaaacaa gtatccgtag
     1261 cctgttttac ctttaatatg gttcccgtat tcgccaattt ttttacgcaa acgggctata
     1321 tttacatcta catttcgttc ggtaacaaca acatcgtcgt cccaaacccg atcgagaatt
     1381 tcttcgcgac taacatagct tccctttttt ttcattaaca gaagaagaat gtcgaactct
     1441 ttgcgtgtca gatcaataat acgtccgtcg atataagcca gctttctcga caggtctatt
     1501 tccagcgtgt cgcagcttat tttttcggtt gatgtatgtt ttcctcgttt aagcactgct
     1561 tttacccggg caatcacttc tttcaccgaa aagggttttt gaatgtaatc gtcgccgccc
     1621 acattgaacc ctgtaagaag atcgttttca ctgcttttgg cggtaatgaa aattatgggt
     1681 atttcaattt gcttttgttt tcgcatagtg tcggccgctt taaaccccga cattccaccc
     1741 atcattacat cgagcaggat gaggtcgtat tcgtgcaggt tctttttaag cgcttcttct
     1801 gctgaatttg cggtatctac ctcatagcct tcgctctcaa ggttgaattg cagaatttcg
     1861 cagagatctt tttcgtcgtc aacaaccaat atttttatat cgtcttctat cattccagta
     1921 atatttttct ttctcgtata gtagtgtcat tggtttatat tcatcatttc gtcgtcgagc
     1981 ggatggtgcc tgatgtcttt tccttccata taataaatgg ctgcttcggc aatgtttgtg
     2041 gcatggtcgg ccattctttc aatattgtcc atcatgtctt tgatggtgat aaagcttatg
     2101 agggttttct ttttatcgtc gaacgatttg ctgcgtgaaa ccattccttt gagcaatttc
     2161 ctgttcatat cgtctaccat atcgtcgttt tttattgtcc agatggcgta ttctttgtcg
     2221 tgatggatga aggaaagcag cgattttgtc accatttgcg tgctggtaat gagcatgggg
     2281 tttatcgcac caatcatttg ttcatacaac tccagatttt ttatctcgct gaagaatttg
     2341 acaatattga ttgtatagtc cccaatccgt tcaaggttaa taattattcg gtaacacgca
     2401 attaactgac ggatatctga ggcaaccggc tggaaaagca cgatggtatt tataatgcgc
     2461 tcgctaagct ttacctcata ttgatcgatt aatttctcgt tggcatcgaa ctcttcgcgt
     2521 aacttttcag gcagattggt ttcacccgac tgaaaaaaac tttccagtct gtcgagttga
     2581 ctgagtacca ggttggccat gctttcaaat tcggtcatga ccttttgtat tgcttcttct
     2641 tttttctgtg acatacaata tttataatta ggttattaac caaattttcc agtaaggtat
     2701 tcttcggttc ggcggtcttt ggggtttgta aacatgattt tggttttgtt atattcaacc
     2761 aattctccaa gatacataaa aagtgagtaa tcagaaatac gggccgcttg cgacatgttg
     2821 tgtgttacca taataatagt gtagttttct tttagcttca ccaccaggtc ttcaatcttt
     2881 gatgttgcaa tggggtcgag tgccgaagtg ggttcatcga gcagtattat ttcggggttg
     2941 aatgccaacg cccgggctat gcaaaggcgc tgttg
//
LOCUS       DAOPUW010000191         2962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_34563_length_2962_cov_3.471620, whole genome shotgun sequence.
ACCESSION   DAOPUW010000191 DAOPUW010000000
VERSION     DAOPUW010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34563_length_2962_cov_3.471620"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PKJ43_04210"
     CDS             complement(<1..265)
                     /locus_tag="PKJ43_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71799.1"
                     /translation="MMSENWDIIAKYLANEELNDEELQIVENLKNDAEGQQLLRESAG
                     VFDDASLYLQQKQYNTLNAWQKVNASVARSARRRLFVISSRVAA"
     gene            complement(324..911)
                     /locus_tag="PKJ43_04215"
     CDS             complement(324..911)
                     /locus_tag="PKJ43_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HNZ71800.1"
                     /translation="MLNDRELFLQIKQGNKKSFEELFMAYYAPLCAFARKYIKDADEC
                     EEIVQSFFTRLWDKRDDLEINLSVKNYLFSSVRNRCINYLKHSKIKVEYQNDFLKSTD
                     TQSDFSDAMLEIDLVEKIEKSIAELPQRRREIFVLSREKGLKYREIADELGISIKTVE
                     AQMGQALKDLRDSLKEYRQLLISFLFVLPLRVSKR"
     gene            complement(1120..1830)
                     /locus_tag="PKJ43_04220"
     CDS             complement(1120..1830)
                     /locus_tag="PKJ43_04220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNZ71801.1"
                     /translation="MGTRILNMEGIGAVTFTRRKGSRRISLRVKPDGSVLVNHPWFAA
                     SNDVLGFVAEHAGWILEQKEKMAGRKIRYQLDEMVQIKNTTIKISRSGVNKLEAGVSP
                     GLVVVAVPVNLPVESDEVQHFIRGVVTLVCRKEAKKYLPERTRQLAQRNDFFFRKVFI
                     KNLKSKWGSCSSQQNINLNLSLMLMPDHLIDYIILHELAHLREPNHGPGFWALLNQLT
                     ENKAKALDREIRKNYRIL"
     gene            2131..>2962
                     /locus_tag="PKJ43_04225"
     CDS             2131..>2962
                     /locus_tag="PKJ43_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNZ71802.1"
                     /translation="MSFTLNINPQSNTLKYLQLVDAVVDALNEGSLSEGDLLPSVNDL
                     MKNAGLSRDTVFKAYGELKNRGIIESVPNRGYFVTRNRNRVLLFLDTFKAYKEVLYDG
                     FRANLPGNISVDLHFHHYNLEVFEQIIDDSLGRYSYYIVMNFDHPEMERVINKIPTEK
                     LLIIDWNIHANEGHSFVCQDFGKSVYDNLKEEIDRIKKYKRFIFLYPEKMTFHPKEAI
                     TNFMKFGSDNQLNFSVIYDPKKIDIQKGDLYLLVSDRTLAMLLDQAAEKNYEPGSDIG
                     I"
BASE COUNT          916 a          654 c          564 g          828 t
ORIGIN      
        1 cagcggcaac cctggaactg attacgaata accttcgccg ggcactgcgg gcaacactgg
       61 cgttcacctt ttgccatgca ttcagcgtat tatattgctt ctgctgcaaa tagaggcttg
      121 catcgtcgaa aacaccagcc gactcgcgaa gcaattgttg cccctcggca tcgtttttca
      181 ggttttcaac gatttgaagt tcctcatcgt tcaactcctc gttagccaaa tatttggcaa
      241 taatatccca attttcactc atcattgttt tatcactcct tcacttcaaa aaaaccattc
      301 ggtttgtata cacaaaagac atttcaacgt ttgcttaccc ttagcggcaa aacaaacaaa
      361 aaagaaataa gcaactgccg gtactctttc aggctgtcgc gcaagtcttt aagcgcctgc
      421 cccatttgtg cctcaacagt cttgattgaa atacccaact cgtcggctat ttcgcggtac
      481 ttaagcccct tttcacggct taagacgaat atttcgcgcc tgcgttgcgg caattcagca
      541 atactcttct ctattttctc aaccaaatcg atctcgagca tggcatcaga aaaatcagac
      601 tgggtatcgg tagactttaa gaaatcattt tgatattcaa ctttaatttt actgtgcttc
      661 aaataattga tacatcgatt gcggactgaa ctaaacagat agtttttcac cgaaaggtta
      721 atttcaaggt catcccgttt gtcccacaac cgggtaaaga aactttgaac aatttcctcg
      781 cactcatcgg catctttaat atattttcgg gcaaaagcac atagcggggc ataataggcc
      841 ataaaaagtt cctcaaagct ttttttgttg ccttgcttaa tttgcaaaaa cagttcccga
      901 tcgttcaaca ttggtaatcg tattaaatat ctatgcaatt tacagaaaaa accattctga
      961 ttatgtttag ccacagtaaa cattgaatag gtagttacat agtttacaaa agcaatcaac
     1021 aacaaatcgt catttcccta tagccattga caactaaaga agttttttcc attactcatc
     1081 ggggaaagaa aaatcaaaaa aatgatgttt aaaggcaatt tataaaatac ggtagttttt
     1141 tcttatttcc cggtcgagag cttttgcctt gttttcggtg agttgattaa gaagcgccca
     1201 aaatccgggg ccatggtttg gctcgcgtaa gtgggcaagc tcgtgaagaa taatataatc
     1261 aataagatgg tcgggcatca gcattaatga aaggttcagg ttaatatttt gctgcgatga
     1321 acaacttccc catttgcttt tcagattttt gataaacact ttacggaaaa agaaatcatt
     1381 gcgctgagcc agttgccgtg tacgttcggg aagatatttt ttggcttctt tacggcaaac
     1441 cagggtaaca accccacgaa tgaaatgctg cacctcatca gactcaaccg gcaaatttac
     1501 tggaactgcc acaacaacca acccgggcga aaccccggcc tcgagcttat tgacccctga
     1561 acggctaatt ttaatggttg tgtttttaat ctgtaccatt tcatccagtt gataacgaat
     1621 ctttcgccct gccatttttt ccttttgctc gagaatccat ccggcatgtt cggcaacaaa
     1681 gccaagcaca tcattcgagg cagcaaacca aggatggttg accaacacac tgccatcggg
     1741 cttcaccctg agcgatatcc ggcgtgaccc ttttctgcgt gtaaaagtta ctgcgccaat
     1801 gccttccata ttcaggatac gtgttcccat agagtttaat ccattcgaaa ataatgtttt
     1861 gtaatgctct ttgccttaac aacaatcaat ctgcggtaaa tatacaaacc aatcccggca
     1921 taactaaagg ggaaaagcat tcatcagccc ccgaaggaaa tatccaccgg caatataatc
     1981 gcgaaaaaaa tgtttacaat gcttgagtgt aaaaattatt tgagacaaag ttttgcaaaa
     2041 gatgttttat aacacaaaaa aatcgttcaa gagctattgc attagaatga aaaagaaact
     2101 tatatttgta ccagaacaga ccagaacagc atgagtttta cgttaaatat caatccgcag
     2161 tcgaatacgc tgaaatatct tcagcttgta gatgctgtgg ttgatgcatt aaacgaaggg
     2221 tcgctatcgg aaggagattt gttaccatcg gtaaacgatc tgatgaaaaa tgccggtctg
     2281 tcgcgcgaca cagtttttaa agcctatggc gaactgaaaa accgaggaat catcgaatcg
     2341 gttcccaacc gtgggtattt tgtcacccgc aaccgaaatc gggtattact gttcctcgat
     2401 acatttaaag cttacaagga agtactatac gatgggttta gggcaaattt gcccgggaac
     2461 atttcggtcg acctgcattt tcaccattac aacctcgaag tattcgaaca aattatagac
     2521 gatagcctgg gccgttacag ttactacatt gtaatgaatt ttgatcaccc ggaaatggaa
     2581 cgggtcatca ataaaattcc aactgaaaag ttgttgataa ttgactggaa cattcacgcc
     2641 aacgaaggac actcatttgt ttgtcaggat tttgggaaaa gtgtttacga caatcttaag
     2701 gaagaaatag atcggataaa gaaatacaaa cgatttattt tcctctaccc cgaaaagatg
     2761 accttccacc ccaaagaagc catcactaat ttcatgaaat ttggcagcga caaccagttg
     2821 aatttttcgg tgatctacga cccaaaaaaa atagacattc aaaaaggaga cctgtactta
     2881 ttggtaagcg accgtaccct ggccatgcta ctcgaccagg cggcagaaaa aaattatgag
     2941 cctggaagcg atattgggat ca
//
LOCUS       DAOPUW010000192         2955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_34685_length_2955_cov_2.703793, whole genome shotgun sequence.
ACCESSION   DAOPUW010000192 DAOPUW010000000
VERSION     DAOPUW010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34685_length_2955_cov_2.703793"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PKJ43_04230"
     CDS             <1..211
                     /locus_tag="PKJ43_04230"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNZ71803.1"
                     /translation="LELLSDNNDLLTIQKTFSGTHRSPLKGIPASGKKVKWHEISVVK
                     FNNDKIIEEWVVSDLAAQLMLKLKS"
     gene            289..1353
                     /locus_tag="PKJ43_04235"
     CDS             289..1353
                     /locus_tag="PKJ43_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNZ71804.1"
                     /translation="MNYKLLSTIAVALLIVLCTTANAQTTSKFDFVPGEKVIFYDDFS
                     QENIGDFPVMWNTNGSGEIVTSANYPGKWLQLTKQGYFIPEATDDFTDNYTIEFDIVF
                     QSTTGDNNVVGIEFYLLSTDLKNPASGSQPGQAGFRMRPEYDNLFWNNWSEAREWQGD
                     DGGVKFTFNTTDKYHFAIWVQKQRVRVYANESKVLDAPKALQAGYKYNVFRTELFSED
                     YSPLISNFRIAAGIPDLRNKLITEGKLVSYGIYFDVNKDEVKPESFATLKDIAQVLSE
                     NPTIRISVIGHTDADGDNAANLDLSKRRAAAVKAYLVKNFNADATRIETDGKGESEPV
                     ADNNTSVNKAKNRRVEFIKL"
     gene            1449..2234
                     /locus_tag="PKJ43_04240"
     CDS             1449..2234
                     /locus_tag="PKJ43_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71805.1"
                     /translation="MNKPIFFLLILVSLIKVSSGQSLFTPGKRLEITLSAGHAQYKEQ
                     LFNGLRNDGVDLSLGLNYGIQKNAMHHEFGWNIDAAGLWNRYGWSSMYLQISNLHYRL
                     LADVGKGLQLGGNVGYSSSFYRNENFDSQHNYWITTLNLGLSTCYSLPINSKWTMMIP
                     VNIPLVGFLSRPAENRNLILNEPDLKIGDILKRINSNFKFVTIGSNYFDIETGLFFRT
                     QLRNEKQVTFGYRVRYEQTATSLKTQLFTNQLCIQYPLSKHQK"
     gene            2231..2881
                     /locus_tag="PKJ43_04245"
     CDS             2231..2881
                     /locus_tag="PKJ43_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNZ71806.1"
                     /translation="MKPIISILILLVMLSSCLKDEDMKLKTAEYLPSEQNDGWILNPV
                     SAENFNMDIFNEVMEAVYSNEDFLLIRSLVVVKDGKLMAEAYPRTLADRKEPHHLWST
                     TKSFISMLTGIAVDKGFIHSVNDSVFTYIPEYMQYAYPELKPLTIEECLTMRSGIDYD
                     NDGWEEEELLACVPNELTRYIVERPMKAKPGAEAFYKNSDPQLLVKVISNAAKTDG"
BASE COUNT          930 a          646 c          607 g          772 t
ORIGIN      
        1 acttgaactt ctttcggaca ataacgatct gctaaccatt caaaaaacgt tttccggcac
       61 ccatcgttca cccttaaaag ggatacctgc atcggggaaa aaagtgaagt ggcacgagat
      121 ttcagtcgtt aaattcaata acgacaaaat aattgaagaa tgggtggttt cggaccttgc
      181 tgcacagtta atgttgaaat taaaaagttg agcattgaga aaaggaaatt aacctattta
      241 aaatcaactc ctcaataaaa aaataagaac caataagatt atatgctcat gaattacaag
      301 ttattatcaa caatcgcagt tgcgctttta attgttttat gcacaactgc aaatgcacaa
      361 actaccagta aattcgactt tgttcctggt gaaaaggtta ttttttacga cgatttttca
      421 caagaaaaca taggcgattt cccggtaatg tggaacacca atggaagtgg cgaaatagtt
      481 acttcggcca attatcctgg gaaatggctg caacttacca aacaaggcta ttttatcccc
      541 gaagcaacgg atgattttac cgataactac accattgaat tcgatattgt ttttcaaagc
      601 acaacaggtg ataacaatgt agttggcatt gaattttacc tgctttcaac cgatttgaaa
      661 aatcccgcaa gcggaagcca acccgggcaa gctggtttta gaatgcgccc cgaatacgat
      721 aatttgttct ggaacaattg gtccgaagct cgtgaatggc aaggcgatga tggtggcgta
      781 aaatttacat tcaatacaac tgataaatac cactttgcca tttgggtaca aaaacaacgg
      841 gtaagggtat atgccaacga aagcaaagtg ctcgatgccc caaaagcctt acaagctggc
      901 tataagtaca atgtattcag aaccgaattg ttcagcgaag attactcacc gctgatctcc
      961 aacttcagaa ttgctgccgg aatccccgat ttgcgcaaca agttaattac cgaaggcaaa
     1021 ttagttagct acggaattta tttcgatgta aataaagatg aggttaagcc agagtcgttt
     1081 gcaaccctaa aagacatagc ccaggtgctg agcgagaacc caacaattcg cattagcgtt
     1141 attggccaca cagatgccga tggcgacaat gcagccaacc tcgacctttc gaaacgaagg
     1201 gctgctgcgg ttaaagcata tttggtgaag aactttaatg ccgatgccac cagaattgaa
     1261 accgatggaa aaggcgaatc agaacctgtt gccgacaaca atacaagcgt taacaaagcc
     1321 aaaaaccgca gggttgagtt cattaaactg taatgacgta ttgttttatt atcaaatata
     1381 aaatctgctg ctcagctggt cgggatttct tcccctaaac ctattcgtta aactatgttg
     1441 aaaaaaccat gaacaaacca attttctttt tactcattct tgtttcattg ataaaagtct
     1501 cctccgggca atcgcttttc actcccggta aaaggcttga aatcacactc tcggccgggc
     1561 atgcccaata caaggaacaa ctttttaacg gtctaagaaa cgatggggta gacctttcgt
     1621 tgggactcaa ttacggaatc caaaaaaatg cgatgcacca tgagtttggg tggaatatcg
     1681 acgcagctgg gttgtggaat cgttacggct ggtcaagtat gtatttgcag attagcaacc
     1741 tgcactaccg tttattagcc gatgttggta aagggcttca actgggtggc aacgtcggtt
     1801 actccagttc gttttacagg aatgaaaact tcgactcaca gcacaactac tggattacca
     1861 cccttaatct gggtttatcg acatgttaca gtttgcccat caattcaaaa tggacaatga
     1921 tgatcccggt aaatattccg cttgtgggtt tcctgagccg accggcagag aatcgtaacc
     1981 tgatactaaa tgaacccgac ctgaaaattg gcgatatact gaaacggatc aacagcaatt
     2041 tcaagtttgt taccattggg agcaactact tcgatatcga aaccggactg tttttccgaa
     2101 cccaacttcg caacgagaaa caagtaacct ttggctacag ggtaagatac gaacaaacgg
     2161 ccacctcgct aaaaacccaa ttgtttacca accaactctg cattcaatat cctctttcaa
     2221 aacatcagaa atgaaaccaa ttatatctat actgattttg ctggttatgc tcagcagctg
     2281 cctgaaagac gaagacatga aactgaaaac ggctgaatac cttccttcag aacaaaacga
     2341 tggctggata ctaaacccgg tttctgccga aaacttcaat atggacattt tcaatgaagt
     2401 gatggaggcg gtttattcca atgaagattt tcttttgatc cgcagccttg ttgtggtgaa
     2461 agatggcaag ctgatggctg aagcatatcc ccgaaccctt gccgatagga aggaacctca
     2521 tcatctttgg tcgaccacca aaagttttat ctccatgctc acaggaatag cagttgacaa
     2581 aggatttatc catagtgtaa acgattcggt ttttacctat atccctgaat acatgcaata
     2641 cgcctaccct gaattgaagc ctctcactat tgaagagtgc ctgaccatgc gttctggcat
     2701 cgattacgac aacgatggtt gggaagagga agagttgctg gcctgtgtgc ccaacgagct
     2761 gacccgttac atcgttgaac gcccgatgaa agcgaagccc ggagcagaag ctttctataa
     2821 aaattccgat ccgcagcttc ttgtaaaagt aatttccaat gcggcaaaaa ccgatggttg
     2881 aatttgccgc ccagaatttt tttgctccgc tgggcattac gaactatcac tggagccgga
     2941 acaaagacaa taccc
//
LOCUS       DAOPUW010000193         2950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_34744_length_2950_cov_4.575475, whole genome shotgun sequence.
ACCESSION   DAOPUW010000193 DAOPUW010000000
VERSION     DAOPUW010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34744_length_2950_cov_4.575475"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PKJ43_04250"
     CDS             complement(<1..199)
                     /locus_tag="PKJ43_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71807.1"
                     /translation="MKHLMIIFLACFFLHASGNEPEGNIYWVQFNTKAGTPYSINQPH
                     NFLSDKALERRKKQSIDIDSTD"
     gene            complement(314..1405)
                     /gene="mnmA"
                     /locus_tag="PKJ43_04255"
     CDS             complement(314..1405)
                     /gene="mnmA"
                     /locus_tag="PKJ43_04255"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNZ71808.1"
                     /translation="MGKGKVLLCMSGGIDSSVAAMLLNDEGYQLHGLTYRPYDVISPA
                     CMEKQTGCCSVDAIFEAKALCNDLKIDHTVLDLRNEFDKTVISDFVKEYLAGRTPNPC
                     VLCNSVIKWGEVIHKADELGCEFIATGHYARIGNENGRHFLIKGEDLSKDQTYFLWQL
                     SQEDLKRTLFPLGHLTKTEVREIAARKGYEKISKKRESQEICFIPDNDYRRFIKERVP
                     NLEEKVGKGNFVDLDGKVLGQHYGYPFYTIGQRKGLEIALGKPRFVIKIDAETNTVTL
                     GDREDLDRKELWVEQINLMKYDKIEGELEVTTKIRYNNQGTLSRISQHGTRIKVEFYE
                     NTWAVTPGQSAVFYEGNHVVGGGIISSSQ"
     gene            1572..2900
                     /locus_tag="PKJ43_04260"
     CDS             1572..2900
                     /locus_tag="PKJ43_04260"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNZ71809.1"
                     /translation="MAYFEDLKLTKSVQKAIAEIGFIEPTPIQEQAIPAIKSGKDVLG
                     IAQTGTGKTAAYILPILTHLVKAEGEDPRVVVLVPTRELSIQVGEDIEELAQFTNIRS
                     VAVYGGIGWTKHAEMIVPGIDILVATPGRLLDLYQHNVLSLKKVKILVIDEADRMLDM
                     GFLPQLKSIFDILPMRKQNLLFSATFNEKVETIAAEFLDFPVRVEVAPSATPVEMVKQ
                     MVYHVPNFKTKLNLVSYLLSDVENFKKVLIFCKTKVSAEEVFKVIKSRSDGEKRILHS
                     NKGQSSRINSISAFKNDEVRILISTDVSARGIDASKVTHVINFDVPTNYDDYIHRIGR
                     TARAGNHGVAITMLDPSEEWHFLNIEKIIRMSVPVYEVPVEVEFGDYGTYEQKDQLRE
                     IDRQKRIDDPTFKGAFHEKKRKLHKDTRSYNDRFVKKESTQAKKKRRLKP"
BASE COUNT          814 a          612 c          628 g          896 t
ORIGIN      
        1 agtcggtaga gtcgatatca atactttgtt tttttcttct ttccaatgct ttgtctgaaa
       61 ggaagttgtg aggctgatta attgagtatg gcgttcctgc tttggtgtta aattgaaccc
      121 aatagatgtt cccttccggt tcgtttcccg atgcatgaag aaaaaagcaa gctaaaaaaa
      181 taatcatcaa atgtttcatc tgcagcaatt tttcgagtat aattccgggt gaaagtcagc
      241 taagcaatta gtcttgatgg ctgaaactat cattgaatta cctgataaag atagaaaaag
      301 agggaaagaa atgctattgg ctgctactta taattcctcc gcctaccacg tggttgcctt
      361 cataaaatac ggcagactgg ccaggtgtta ctgcccaggt gttttcataa aattcaacct
      421 tgatccgggt tccatgttgc gaaatccggc tgagagttcc ctggttattg tagcgtattt
      481 ttgttgttac ttctagttcc ccttcaatct tgtcgtattt cattaaattg atttgttcaa
      541 cccataattc tttgcggtcg aggtcttcac ggtcgccgag ggttactgta tttgtttccg
      601 catcgatttt tattacaaaa cgtggttttc ctaaggctat ttcaaggcct ttccgttgcc
      661 cgatggtgta aaacgggtac ccatagtgtt gccctaagac tttcccatcg aggtcgacaa
      721 aatttccttt tccaactttt tcttccagat taggaacccg ttctttgata aaacgccggt
      781 agtcattgtc gggaataaaa caaatctcct ggctttccct tttttttgag attttttcat
      841 agcctttccg ggccgcaatt tcacgtactt cagttttggt aagatgacca agcgggaaca
      901 aggtgcgctt caggtcttcc tgagaaagtt gccataggaa atatgtctgg tctttactca
      961 ggtcttcgcc ctttatcaga aaatgacgtc cgttttcgtt tccaattcgg gcatagtggc
     1021 ctgtggcaat aaactcacaa ccaagttcgt cggccttgtg gattacttct ccccacttga
     1081 ttacactgtt gcacaacaca caagggtttg gagtccggcc tgcaaggtat tctttaacaa
     1141 aatcggatat tactgttttg tcgaattcgt ttcgtaagtc gagtacagta tggtcaatct
     1201 tgaggtcgtt gcacaaggct ttggcttcaa aaatggcatc gacactgcaa cagccagttt
     1261 gtttttccat gcatgcaggc gaaattacat cataaggcct gtaggttagc ccgtgcagct
     1321 ggtagccttc atcgttgagc aacattgctg ccaccgaact gtcgatgcca ccgctcatgc
     1381 ataacaatac ttttccttta cccataaaac gttttgaaat aactgtgcaa agaaacaaaa
     1441 atacaataag aatgtttgtt actgaattct tatttttcgg gtggtattgt ttgtaatgtg
     1501 ttgtaatgta ggaatatgtg tagaattaga tgaaccggat tcttttcctt atttttgcga
     1561 aaaatttcac tatggcatat ttcgaagatt taaaactaac aaaatcggtt caaaaggcaa
     1621 ttgcagagat tggtttcatc gagccaacgc ccattcagga acaagcgatc ccggccatca
     1681 aatcggggaa agatgttctt ggcattgccc aaaccggaac tggaaaaact gcagcttaca
     1741 ttttgccaat cctcacacac ctggtcaaag ctgaaggtga agatccacgg gttgtcgttc
     1801 ttgttcctac ccgcgagctg tcaattcagg taggcgaaga tattgaagag cttgctcagt
     1861 ttaccaatat ccgaagtgtt gctgtttatg gtggaattgg ctggaccaaa catgccgaga
     1921 tgattgttcc cggaattgat atcctggtgg ccacaccggg gcgtttgctc gatttatatc
     1981 agcacaatgt gttaagcctc aaaaaagtga aaattttggt tattgatgag gccgaccgga
     2041 tgctggacat ggggttttta cctcagctga aatcaatttt cgacatcctt cccatgcgga
     2101 aacaaaactt gttattctcg gcaacattta atgaaaaagt tgaaaccatc gccgctgaat
     2161 ttctcgattt tccggtcagg gttgaggttg ccccgtcggc aactcccgtc gaaatggtga
     2221 agcagatggt gtatcatgtt ccaaatttta aaacaaaact taatcttgtt tcttatttat
     2281 tatctgatgt tgagaatttt aagaaagttt taatcttttg caaaaccaaa gtgtcggctg
     2341 aagaggtttt taaagtcata aaaagccgtt ccgacggaga gaagcgtatc ctgcattcga
     2401 ataagggaca gagttcacgc ataaattcta tttcggcttt caaaaacgac gaagtgcgta
     2461 ttctcatctc caccgatgtt tcggccaggg gaattgatgc cagtaaggtt acgcatgtta
     2521 tcaatttcga tgtgccaaca aattacgatg attatatcca ccgtattggc cgtacagcac
     2581 gggccggaaa ccatggcgtg gctatcacca tgctcgatcc ttccgaagaa tggcatttcc
     2641 tgaatattga gaagattatc cgcatgtcgg ttcctgttta tgaagttcct gttgaggttg
     2701 aatttggcga ttacggaaca tacgaacaaa aagatcagct tcgcgaaatc gaccggcaaa
     2761 aacgcatcga cgacccaacg ttcaaagggg catttcacga aaagaagcga aaactccata
     2821 aagatacccg ttcgtataat gatcgttttg tgaaaaagga atcgactcaa gcgaagaaaa
     2881 aaaggcgctt gaagccataa caaaattata tttttcataa aaagtcatct ccgaacgtac
     2941 ctttttctta
//
LOCUS       DAOPUW010000194         2947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_34780_length_2947_cov_3.821923, whole genome shotgun sequence.
ACCESSION   DAOPUW010000194 DAOPUW010000000
VERSION     DAOPUW010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34780_length_2947_cov_3.821923"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..627)
                     /locus_tag="PKJ43_04265"
     CDS             complement(214..627)
                     /locus_tag="PKJ43_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71810.1"
                     /translation="MNSIKPRVIKDYDKLPLEIQEQIKLEYPFGFSDNLIRFTNKDGL
                     IVSALPFETEDKYYMVRMTVGEAIKIVEDDDDFDDDGILKEGIKEEYANKYGEELEGM
                     EVSEEEDSGYDDDYDDDDYDDDDADEEVADDDDED"
     gene            949..2478
                     /gene="gpmI"
                     /locus_tag="PKJ43_04270"
     CDS             949..2478
                     /gene="gpmI"
                     /locus_tag="PKJ43_04270"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNZ71811.1"
                     /translation="MADNKKAMLMILDGWGIGNGSKSDIISTAPTPFIDTLLNEYPHS
                     KLLASGEYVGLPDGQMGNSEVGHLNLGAGRVLYQDMVRITRAIKDKSLWSHPQILKAY
                     NYAKDNNKKIHLVGLIGPGGVHALSTHMVALCQIATEMGLKDIFIHGLTDGRDTDPRS
                     GYGFLESDLEAIKSTNARFASLIGRYYGMDRDKNWDRMKLAYDLWLSGKGEKSSNLLE
                     TVKNFYNEGTTDEFMKPIVMVDELGNPLATFEEGDVVLCFNFRTDRLRQATIAFTQQD
                     LPEFGMKTQNLEWYTLTEYKADFKGINVIFGKDNVTNTIGEVISNEGLKQIRIAETEK
                     YAHVTFFFSGGQEKEFPGEKRILCPSPKVATYDLKPEMSAYDIRDAIVPELKNKTADF
                     VCLNFANGDMVGHTGVYEAIYKAVVAVDECVKDVVTAAKEGGYDILIIADHGNADNAI
                     NEDGSPNTAHSLNPVPCIWVTDRKNGKISDGVLADVAPTLLSIMGLNIPESMTGKVLI
                     S"
     gene            complement(2583..>2947)
                     /locus_tag="PKJ43_04275"
     CDS             complement(2583..>2947)
                     /locus_tag="PKJ43_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71812.1"
                     /translation="MRKPEIIHYKGKDVVYIDLSNLKSNDEIIQLEKQASEMIRSRPV
                     KSVYTLTNMEGMFFNNDIKSFFEDAVKHNAPFVCCGAVIGLSGLITIFYNAFLKITGR
                     NIKSFRTKEEALEYLAMS"
BASE COUNT          917 a          625 c          565 g          840 t
ORIGIN      
        1 ggaatgcaca ttggccccat gcggccgcag caaaccatca cgttttcgtt ctctgaaata
       61 atttttcaga agtcgacttt cgaaatgata tgttctaaac ttgtgtgtaa catttttatt
      121 gaattaaaat ttcaacaaaa atacacacac atacctcttc agccggtaac aaaagtcaca
      181 aagcaggaac aaaaaaatcc cggttattcg ggattaatct tcgtcatcat cgtcggcgac
      241 ctcttcatca gcatcgtcgt cgtcgtaatc atcgtcatcg taatcgtcat catatcccga
      301 atcttcttcc tctgacactt ccataccttc gagttcttcg ccatatttat tggcatactc
      361 ctctttgatt ccttctttaa ggataccatc atcatcgaaa tcgtcgtcgt cttcaacaat
      421 tttgatggct tcgccaacgg tcatccttac catataatac ttatcttcgg tttcgaatgg
      481 caaagcactc acgataaggc cgtctttatt tgtaaagcga attaagttat cgctaaaccc
      541 aaaaggatat tccagtttta tttgttcctg tatctccaaa ggcagcttat cgtaatcttt
      601 aattacccgt ggtttaatac tattcattgc aaaaggttta taaggttata cgctcaaatt
      661 aatcaaacaa tactctgtgt tagagtaaaa aaacatgagt atcaactttg cagttttgct
      721 tgtttttatg cgggcaaaat acaaaacttt tataaaccta cgcaaggata ttaaaaaatg
      781 aatgtttttt tttctgatta tttttgctcc ggcaagcaca accagctata attgggtgaa
      841 tttaagccac aattaacaat tttctgaaat caaagttggg tcgatggttg ttgtattaaa
      901 actgatactt ttgcaaagca ttataacata caataaaata aatactttat ggctgacaat
      961 aagaaggcaa tgttgatgat ccttgacgga tggggaattg gtaatggaag caaatctgat
     1021 ataatttcta ctgcaccaac accttttatc gacacactgt taaacgaata cccacactca
     1081 aagcttcttg caagcggtga atatgtaggg ctgcccgacg ggcaaatggg aaactcagaa
     1141 gttgggcacc taaatttggg cgccggaagg gttttatatc aagatatggt tcgcattacc
     1201 cgtgccataa aagacaaatc gctgtggagc catcctcaga ttctgaaagc ttacaattac
     1261 gcaaaagaca acaacaagaa aattcatttg gtaggactta ttggccctgg aggggtacac
     1321 gcccttagca cccacatggt tgcactttgc caaattgcca ccgaaatggg actaaaagat
     1381 attttcattc atggccttac tgatggccgt gatactgatc cccgctcggg ttacggcttc
     1441 ctcgaaagtg atcttgaagc tattaaaagt acaaatgccc gctttgcttc gctcattggc
     1501 cgttactacg gtatggaccg cgataaaaac tgggaccgga tgaaattagc ctacgatctg
     1561 tggctgagcg ggaaaggtga gaaatcgtcg aatttactcg aaactgtgaa gaatttttac
     1621 aatgaaggga caaccgacga attcatgaaa cccattgtta tggttgacga attggggaac
     1681 cctcttgcta ccttcgaaga aggcgatgta gtactttgct tcaatttcag aaccgaccgc
     1741 ttgcgtcagg cgacaattgc attcactcag caagaccttc ccgaatttgg aatgaaaacc
     1801 caaaatttag aatggtacac ccttaccgaa tacaaagccg acttcaaagg aatcaatgtg
     1861 atttttggca aagacaatgt aaccaacacc attggagaag taatatcgaa cgaagggtta
     1921 aaacaaatca gaattgcaga aactgaaaaa tatgcgcacg taacattctt cttctcgggc
     1981 ggacaggaaa aagaattccc gggtgagaaa aggattttat gcccttcgcc taaagtggca
     2041 acctatgacc tcaagcccga aatgagtgct tatgatatac gcgatgctat tgtgcccgaa
     2101 ttgaagaaca aaacagctga ttttgtttgc ctcaactttg ccaatggcga catggttggc
     2161 cacaccggag tatacgaagc tatttataaa gcggttgttg ctgtcgacga atgcgtgaaa
     2221 gatgttgtta ctgctgccaa agaaggtgga tatgacatac tgatcatagc cgaccacgga
     2281 aatgcagaca atgctattaa cgaagacggg tctccaaata cggctcactc gcttaaccca
     2341 gtaccttgca tttgggttac tgaccgcaaa aacggaaaaa tttcagatgg tgttttggcc
     2401 gatgttgccc ctaccctgct ttcgatcatg ggattaaata tccccgaaag catgacaggt
     2461 aaagtactga tttcgtaaac aatatactct ataattaaaa aactccggca gctatctgtc
     2521 ggagtttttt gcataataaa aatcggttgc ccacaaaggt tctaagcagg caatacttaa
     2581 gattaactca tcgccaaata ctcaagagcc tcttctttag tcctaaagga cttaatatta
     2641 cgaccggtaa ttttcaaaaa tgcattgtaa aaaatagtta taagcccaga aagaccaata
     2701 acagccccgc aacaaacaaa aggagcattg tgcttcactg catcctcgaa aaaactttta
     2761 atgtcattgt tgaaaaacat cccctccatg ttcgtcaacg tataaaccga tttaaccggg
     2821 cggcttctaa tcatttcaga agcttgtttt tctaattgga tgatttcatc gttgcttttt
     2881 aagtttgaaa ggtcaatata aacgacatct ttccccttgt agtgaataat ttcaggtttt
     2941 ctcattc
//
LOCUS       DAOPUW010000195         2947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_34790_length_2947_cov_2.330913, whole genome shotgun sequence.
ACCESSION   DAOPUW010000195 DAOPUW010000000
VERSION     DAOPUW010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34790_length_2947_cov_2.330913"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2947
                     /locus_tag="PKJ43_04280"
     CDS             <1..>2947
                     /locus_tag="PKJ43_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71813.1"
                     /translation="TYTETKNSKTATCNETIDFTYEGPKTTLVIANAVAKTYIPSIVV
                     DAADVVAEAGQTYNYNFADTSVFEQVTTTKYSTFVTADGIVAMKNNDGAQFWWHDTQH
                     GAAMYVANSIAVTVAGNAKITIGTCQYSMSDVVLEFADLEGNVLGSIPATDKGTGACS
                     SHSFNYNGEAGTITGTFKNNGTSTGTIYIHNVTIENEKAKTAMIDSWDFGAAQLDTTK
                     YSNKLTESLINSWYDASIAIGSTGVTLPASFIVDELRWVGAANKDRLRTTNENLTRYD
                     ATGAPIIIEGDTLTGSLYVNASASMARYLTIDANEDDEVYIYCKSQNAAGLINFVYSD
                     PTIQTDTASSSSNGVKIRFVAKNTGTYKIYDTKDKPFYYRILRKDATMVKVSGAVDVT
                     KAPGIPAGYKIEFKNAAGKTWNASVSGSLFSTKLPAGFEYSLSLIGANGYIINNGSKL
                     TIESETTHDVSIEKLEMFTASGSIIGLDSTQLKKLALVYTPATEKIYKPETQINASSA
                     TYSSNLEPNCLYTISATGVNDFVISDDTIMIGKANATASITFVAKPVYPVTITTEGLA
                     DSLKAKLKLTFTNLKETGYTYTFADLNNIALRDGDYAVSCSGIEAYPIELGATSNVKI
                     ARASSSKTLSFKTVTSWPFDDATITNKDAAYKGLLFTGNIFNEKAKSHLVAKASATIN
                     VPMNPNEKLIIGYYYAAAFSINGGDTIRTTSNSTSIIESTSYLYTGTEAGYVTIGTGS
                     GTTYIADMSVIKVIPYVETITVGVDKNYQTINGALEAVRSMIRPNNERVSIVIDPGNY
                     EEMLVIDVANVSFTNAAEEPSIALLNKGVDIDKNAVRITSYYGHGYNYYSMATNQKWS
                     ADALRVNKENGYSTYTNTGGASTSGSYWNATVVVSAPGFEAANIIFENSFNQYISKKE
                     SEDVVVEWAVGGKGKRPTDIGNTAVQAKSFVERAAAIAYTKSGDRSILNNCRVVGRQD
                     SFYGAEGARV"
BASE COUNT          983 a          646 c          597 g          721 t
ORIGIN      
        1 gcacttacac cgaaacaaaa aactcaaaaa cagcaacttg caacgaaacc attgacttta
       61 cttacgaagg accaaaaacg actttggtaa ttgcaaacgc agtagccaaa acttacattc
      121 cttctattgt tgttgatgct gccgatgttg ttgctgaagc aggccaaacc tacaactaca
      181 actttgccga tacatcagtc ttcgagcaag ttacaacaac caaatactca acttttgtta
      241 ctgctgatgg tattgtagcc atgaaaaaca atgatggtgc acaattttgg tggcacgaca
      301 ctcaacacgg ggcagcaatg tacgtggcca attcaatagc cgtaaccgtc gcagggaatg
      361 ctaaaatcac aataggaacc tgccaatata gcatgtccga tgttgtactg gaatttgctg
      421 atttggaagg caatgtatta ggatcaatac ctgccaccga caaaggaacc ggggcttgca
      481 gttcccattc gttcaattac aatggcgaag ctggaactat aacaggaacg ttcaaaaaca
      541 acggtacctc gaccggaaca atctacatcc acaatgtgac aatagaaaat gaaaaagcca
      601 aaacagccat gattgactca tgggactttg gagcagctca attagataca acaaaatact
      661 cgaataaact tacagaatca ctcattaaca gttggtatga tgcttcaatt gcaataggtt
      721 caacgggggt tacactccca gcatcgttta ttgttgatga gctaagatgg gttggtgcag
      781 caaacaaaga ccggttaaga acaacaaacg aaaatttgac ccgctatgat gctaccggag
      841 ctcctatcat aattgaagga gatacgctta ccggctcgct ctacgtaaat gcaagtgcct
      901 caatggctag gtatcttaca atcgatgcca atgaagacga cgaagtttac atttactgta
      961 aatcgcaaaa cgccgccgga ttaatcaact tcgtctattc cgacccaaca atacaaaccg
     1021 ataccgcatc gtcgagtagt aacggggtta aaatcagatt cgttgcaaaa aataccggga
     1081 catataaaat ctacgacaca aaagataaac ctttctacta tcgtattcta agaaaagatg
     1141 ccaccatggt gaaggtttcg ggtgctgtag acgtaactaa agcccctggc attcctgccg
     1201 gatacaagat tgaattcaaa aatgcagccg gaaaaacctg gaacgcctct gtttcaggct
     1261 ctttgttcag cacaaaactc cctgcagggt tcgaatactc actaagcctt attggcgcca
     1321 acggttatat catcaacaat ggatcgaaat tgaccattga aagcgaaacc actcacgatg
     1381 tttcaattga aaagctagaa atgttcactg ctagcggttc aatcattgga ttagattcga
     1441 ctcaattaaa aaaactagcc cttgtatata ctccggcaac cgagaagatt tacaagcccg
     1501 agactcagat caatgctagt tctgcaacct attcttccaa tctggaacct aattgcttat
     1561 atactatttc agcaacagga gtaaacgatt tcgttatttc agacgataca attatgatag
     1621 gaaaagctaa tgctacagcc agcatcactt ttgtggcaaa accagtttat ccggtaacca
     1681 ttaccaccga aggtttggcc gactcgttga aagccaaatt gaaactgact ttcacaaatc
     1741 tgaaagaaac aggttatacc tacacttttg ctgatcttaa taatattgct ctcagggatg
     1801 gtgattacgc tgtatcatgc tctggaattg aagcctatcc aattgaattg ggcgctacat
     1861 cgaatgtaaa gattgcaaga gcttcaagct ccaaaacact ttcattcaaa actgtaacat
     1921 catggccatt cgacgacgca accattacca ataaagatgc agcatacaaa ggattgctat
     1981 tcaccggaaa tatctttaac gaaaaagcca aaagccattt agttgcaaaa gccagtgcaa
     2041 ccattaatgt accaatgaat ccaaacgaaa aactcatcat tggttactac tatgctgctg
     2101 ctttctcaat taacggaggc gacaccatca gaacaacaag caacagcacc agcattatcg
     2161 aaagcacatc gtacttatac acaggtaccg aagctggcta tgttacaatt ggaaccggat
     2221 caggaacaac ctacattgcc gacatgtcgg tgattaaagt aattccttat gtcgaaacaa
     2281 ttaccgttgg ggttgataaa aattaccaaa cgataaacgg agctttagag gctgtacggt
     2341 ctatgatcag accaaataac gaaagagttt caattgtaat cgacccggga aactatgaag
     2401 agatgctggt tattgacgtt gccaatgtgt ctttcaccaa tgcagccgaa gaaccaagca
     2461 ttgcgctcct caacaaaggt gtcgatattg ataaaaatgc agtcaggatt acttcgtact
     2521 acggacatgg ctataattac tacagcatgg caacaaatca gaaatggagt gccgatgcat
     2581 tgcgggtcaa caaagaaaac gggtattcaa cctacacaaa tactggtgga gcatcgacca
     2641 gcggttcgta ctggaatgca acagttgtgg taagtgcccc gggatttgaa gctgccaaca
     2701 tcatcttcga aaactcgttc aaccaataca tttcgaaaaa agaatcggaa gatgttgttg
     2761 ttgaatgggc tgttggtgga aaaggcaaac gaccaaccga cattggcaac actgcagttc
     2821 aggcaaaaag ttttgttgaa agagccgcag ctattgctta cactaaaagt ggcgacagat
     2881 ctatattgaa caactgtagg gttgttggcc gtcaggactc gttctatgga gctgaaggtg
     2941 ccagggt
//
LOCUS       DAOPUW010000196         2945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_34820_length_2945_cov_3.219377, whole genome shotgun sequence.
ACCESSION   DAOPUW010000196 DAOPUW010000000
VERSION     DAOPUW010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34820_length_2945_cov_3.219377"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1879
                     /locus_tag="PKJ43_04285"
     CDS             <1..1879
                     /locus_tag="PKJ43_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HNZ71814.1"
                     /translation="LLDGLFKINSIDTVTISVHSENFRRLLLALSGDLRVILIKIADR
                     LEDMRNLDGLPSDLQQKYANESSFLYAPLAHRLGIYNIKTELEDASLKYLKPDEYQNI
                     VKKLEDSLEERKNFVSEFVKPIEAKLQARNFKFEMKARTKSIYSIWNKMKKQRVTFEE
                     VFDLFAIRIILDSKLEEEKSDCWQVYSIVTEEYQPNTERMRDWISIPKSNGYESLHTT
                     VMGPHGRWVEVQIRTRRMDEVAEKGLAAHWKYKGGKSSGELDKWLAGIREMLENPSGD
                     TNAIIEEFKTNVYDDEIFVFTPKGDLKRLPAGATILDFAYEIHSGLGDKCVGGKVNQR
                     KVTIKHKLKNGDQVAIETSSNQKPKIDWLDFVVTSKAKSRIKASLNEDKRREAENGKE
                     IVKRKFKNWKIDYSEENIRLMISHFKLKSALVLYYAVSVGQIDPLELKSFIRGTEIQE
                     VKAKEVLGELLPKKVVENLSFDSGDDFLVIDKDLKNVIYKLAQCCNPIFGDEIFGFVT
                     IREGIKIHREHCPNAKQMRERYPYRFIKAKWRSTKQNASFQATIHLSGSDRQGIVGDI
                     SYIIAKDVGVQMRSINVDSKNGNFEGTLRVYVNNVEHLDFLMHKMKNIKGIQHVSRGD
                     Y"
     gene            1879..>2945
                     /locus_tag="PKJ43_04290"
     CDS             1879..>2945
                     /locus_tag="PKJ43_04290"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNZ71815.1"
                     /translation="MKIVIAPNSMKGSLDAFAFCDAIEKGLRISGITNIEKLPLADGG
                     DGTAAILANVYNANIQHITVCDPLERKIESGYYLTPDSQIAIIGMADASGLHLLKRYE
                     YSATQSTSYGTGELINGAIEAGAKTIIIGLGGSATVDGGMGALMALGVRFYGHNGEIK
                     SGCGLRLGDVLHIDRTDALLRLKNVELILLTDVKTPMTGINGAAKLFGPQKGATEGDV
                     EILSKNLDIFAQKLFQETKKDVSFLPGGGSAGGIAASFHALLGAKIENGAPFILEKTS
                     LMHKAIHSDFIITGEGKIDETTFEGKLPGEVLKIGMKIDKPVFAICGATTLKDEEGFE
                     AVLPINKLSRSEEESTEHASD"
BASE COUNT          995 a          548 c          646 g          756 t
ORIGIN      
        1 gctcctcgac ggactgttca aaataaactc tatcgacaca gtcacaattt cggtgcattc
       61 cgaaaatttc aggagactac tgcttgccct ttctggcgac ttgcgggtaa ttcttattaa
      121 aattgccgac cgtctcgaag atatgcgaaa cctggacgga ctcccatccg acctgcaaca
      181 aaaatatgct aacgaatctt cgtttctgta tgctccgcta gctcataggc ttggtattta
      241 caatattaaa actgaactgg aagatgcatc gttgaaatac cttaaacctg atgaatatca
      301 gaatattgtt aaaaagcttg aagattcttt ggaagaacgt aaaaattttg tttctgaatt
      361 tgtaaaacct attgaagcaa aattacaggc aagaaatttc aaatttgaaa tgaaagcccg
      421 gactaaatca atttattcta tttggaataa aatgaaaaaa caacgggtaa ccttcgaaga
      481 agtattcgat ctttttgcca tccgcatcat tctcgactca aaactagaag aagaaaaatc
      541 agactgctgg caagtatatt ccatcgtaac cgaagaatat cagccaaata cagaacgaat
      601 gcgagactgg atttccattc ccaaatcaaa tggatacgaa tcgttgcaca caacggtaat
      661 ggggcctcac ggtcgttggg tcgaagtgca aatacgaacc cgaagaatgg atgaagtcgc
      721 agaaaaaggg ctggctgctc actggaaata taaaggtgga aaaagcagcg gagaactcga
      781 caaatggctc gcaggcatta gggaaatgct tgaaaaccct tcgggagata ccaatgccat
      841 aattgaagaa ttcaaaacca atgtatatga cgacgagatt tttgttttta ctcctaaagg
      901 cgacttaaaa cgcttaccag ccggggcgac aattctcgat tttgcttacg aaattcattc
      961 ggggcttggc gataaatgtg ttggaggaaa agttaaccag cgaaaagtca ccataaaaca
     1021 caagcttaag aatggtgatc aggttgccat tgaaacatca agcaaccaga aacccaagat
     1081 cgattggctt gactttgtgg ttacttcaaa agccaagagc cgtatcaaag caagtttgaa
     1141 cgaggataaa cgacgtgaag ctgaaaatgg gaaggaaatc gttaaacgga aattcaaaaa
     1201 ctggaaaata gattacagcg aagagaatat ccgactgatg atcagtcatt tcaaattgaa
     1261 atcggctttg gttctgtatt acgcagtgtc ggtcggacaa atcgacccgc ttgaactgaa
     1321 aagttttatt cgcggcactg aaatacagga agttaaagcc aaagaagttc tcggagagct
     1381 tttgccgaaa aaagttgttg aaaacctttc attcgactca ggtgatgatt ttctggtgat
     1441 cgacaaagac ctgaaaaatg taatttacaa attggcccaa tgctgtaacc caatatttgg
     1501 agacgaaatt ttcggttttg tcaccattcg cgaagggata aaaatacatc gtgagcactg
     1561 tcccaacgcc aaacaaatgc gtgaaagata tccatatcgt ttcattaaag ccaaatggag
     1621 atcgaccaag caaaatgctt catttcaggc aacaatacac ctttcaggct ccgacaggca
     1681 ggggattgtc ggcgacattt cttatatcat tgcaaaagat gttggggtgc aaatgcgttc
     1741 aataaatgtc gattcaaaaa atggcaattt tgaaggtacc ctcagagtat atgtaaacaa
     1801 tgtggaacat cttgattttc tgatgcataa aatgaagaat atcaaaggga tccaacacgt
     1861 ttcaaggggc gattattgat gaaaatagta atagcaccca attcgatgaa aggttctctc
     1921 gatgcttttg cattttgcga tgcaatagaa aaaggactca gaatatcggg aataacaaat
     1981 attgaaaaac ttcctctagc cgatggaggc gatgggacag ctgctattct tgcaaatgtt
     2041 tataatgcaa atatacagca tattacagtt tgtgacccgc ttgaacgaaa gatagaaagc
     2101 ggttattatc ttacaccaga tagccaaata gcaattattg gaatggccga tgcatcgggg
     2161 ttacacctgc tcaagcgtta tgaatactct gccactcaat cgaccagcta tggcaccggt
     2221 gaactcataa atggagctat tgaagccggg gcaaaaacaa taattattgg tctaggaggt
     2281 agtgcaactg tcgatggcgg catgggggcg cttatggctt tgggtgttag gttttatgga
     2341 cataatggag aaatcaaatc aggatgcgga ctaaggttgg gagatgtgct tcatattgat
     2401 agaaccgatg ctttactgcg gttaaaaaat gttgaattaa tcttgctaac cgatgtaaaa
     2461 acacccatga caggcattaa tggagcagct aagctttttg ggcctcaaaa aggagccaca
     2521 gaaggcgacg tggaaatatt atcaaaaaat ttagacatct tcgcacagaa attattccag
     2581 gagacaaaga aagatgtaag cttcttaccc ggtggtggtt ctgcgggggg tattgccgct
     2641 tcatttcatg ctctattggg tgcaaaaatc gaaaatggag caccgtttat cctcgaaaaa
     2701 accagtctta tgcacaaagc aatccattca gatttcatca ttaccggcga aggcaaaatt
     2761 gatgaaacta cctttgaagg taaacttcca ggagaggttt taaaaattgg aatgaaaatt
     2821 gataaaccag tatttgcaat atgtggcgcc accacgttga aggatgaaga aggtttcgaa
     2881 gcggttttgc caattaataa actttcaaga agtgaagaag aatcgacaga acatgcttca
     2941 gattt
//
LOCUS       DAOPUW010000197         2942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_34870_length_2942_cov_2.922411, whole genome shotgun sequence.
ACCESSION   DAOPUW010000197 DAOPUW010000000
VERSION     DAOPUW010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34870_length_2942_cov_2.922411"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="PKJ43_04295"
     CDS             complement(<1..1041)
                     /locus_tag="PKJ43_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNZ71816.1"
                     /translation="MQLIEQTLGNYLEKWAFETPDNDFMVYADRGLRFSYSEFNQRVD
                     RLAKGMLYIGIKKGDHVGVWATNVPDWLTYFFAAAKIGAVLVTVNTSYKTHELEYLMS
                     NSDLHTLCLIDGWRDSDYLGMVSELVPELKTSERGFLKSERFPCLKNVIYIGPEKHRG
                     MYNTQELILLGEHIDDTELEDAKAEVDPYDVVNMQYTSGTTGFPKGVMLTHVNILNNG
                     YSIGDRQKFTNKERVCLPPPLFHCFGIVLGVMAVLSHGAALVMIETFDPLLVLASVQK
                     EKATALYGVPTMFIAELNHPMFNMFDLSTLRTGIMAGSLCPIETMRAVIDKMYMKDIT
                     IVYGLTESSPGMT"
     gene            complement(1053..1628)
                     /locus_tag="PKJ43_04300"
     CDS             complement(1053..1628)
                     /locus_tag="PKJ43_04300"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNZ71817.1"
                     /translation="MSSKKNVGEKIKQIRELKNISIEDLAMRSNLDVVQITQIEENKV
                     VPSLAPLIKIARSLGVRLGTFLDDSEQLGPVVTLAGEQQKGASFSNKNLKARSHMDFF
                     ALASDKAGRHMEPFIVEIKPGDVNDYMLSSHEGEEFIYVLEGCIEINYGKDVYVLTQG
                     DTIYYDSIVNHNVHAGSNCTAKILAVVYAPF"
     gene            complement(1846..2568)
                     /locus_tag="PKJ43_04305"
     CDS             complement(1846..2568)
                     /locus_tag="PKJ43_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71818.1"
                     /translation="MKTITYKTRILATAIAILVVAFTATEANAQRRTLENAKASNERV
                     EKKDNPKSRLANNSVKEPKKTEKQSFDNNRGKGHGNDKGRDGRYENGNGNRGKGNNGR
                     DGVRDERHGRDKHNDEYGRNDGHRGNGYHSSHNNRPKYVHVNPPRVHPNDYIWHHHKN
                     VRYRHLPRKAVWIAIDGMNYAYYKGRFYLPGPFGFYKVTPPTYLHNLPGGCIRVHVDG
                     TPMWGLHGILFIETPFGFKVIV"
BASE COUNT          825 a          653 c          584 g          880 t
ORIGIN      
        1 ggtcattccc ggcgacgatt cagttagccc gtaaacaatc gtaatgtctt tcatatacat
       61 tttatcgatt accgcgcgca tggtttctat tggacaaaga gatccggcca tgatccccgt
      121 acgcaatgtg ctaaggtcga acatattgaa cataggatga ttcagctcgg caataaacat
      181 ggtaggtaca ccgtacagtg ctgttgcttt ttctttttgc accgaagcaa gcacaagcaa
      241 tggatcgaaa gtttcaatca tcaccaaggc agccccatgc gaaagtacag ccattacccc
      301 aagcacgata ccaaaacaat ggaacaacgg aggaggcaaa caaacacgtt ctttatttgt
      361 aaatttttga cgatcgccaa tagagtatcc attgttcagg atgttcacat gggtgagcat
      421 tacgcctttc ggaaaacctg ttgttcccga tgtatattgc atattcacaa catcatacgg
      481 atcaacttca gcctttgcat cttcgagttc tgtatcgtcg atatgttcgc ccaacaaaat
      541 aagttcctga gtgttataca tcccgcgatg tttctctggg ccaatgtata tcacattctt
      601 caggcacgga aaacgttcgc ttttgaggaa gccacgttcc gatgttttca gttcaggcac
      661 aagctccgaa accatcccaa gataatcgct atcacgccaa ccatcaatta aacacaaggt
      721 gtgcagatcg gagttcgaca tgagatattc tagttcgtgc gttttataac tggtattcac
      781 cgtaaccaat acggcaccaa tttttgctgc agcgaaaaag taagtcagcc agtcgggtac
      841 attggtagcc caaaccccca catgatctcc tttttttatg ccgatatata acatcccttt
      901 ggccaatctg tcgactcgtt ggttaaactc gctgtacgag aacctcagcc cgcggtcggc
      961 atacaccata aaatcattgt cgggcgtttc aaaggcccac ttttcgaggt aattacccag
     1021 cgtttgttca attaactgca tgtgacaaat aattagaatg gtgcataaac aactgccagt
     1081 attttagccg tgcaattgct tccggcatga acgttatgat taacgattga atcgtagtag
     1141 atagtgtcgc cttgagtgag cacataaaca tctttcccat agtttatttc gatgcaacct
     1201 tcaagaacat aaataaattc ttcgccctca tgcgacgaca acatataatc gtttacatcg
     1261 cctggtttaa tttcaacaat gaagggctcc atatgccggc ctgccttatc gctggccaac
     1321 gcaaaaaagt ccatgtgtga acgggctttc aagtttttat tagagaaact ggcacctttt
     1381 tgttgttcgc cagccaaggt aactacaggc cccaattgct cgctgtcgtc gagaaatgta
     1441 cctagccgta cgcctaacga acgagctatt ttaatgagcg gtgccaacga aggaaccact
     1501 ttgttttctt caatttgggt tatttgtacc acatcgaggt tactgcgcat ggcaagatct
     1561 tcgatactga tattttttag ctcgcggatt tgtttaattt tttcacctac attttttttc
     1621 gatgacatag tacttttgat ttaaggttga atgctaacaa aaatagaatt aaggtattaa
     1681 tttgaaaata aaacattgaa atattctcaa tttttttatt caaaaaggcc attttcatca
     1741 aatttgtaag gaaaaaacaa aaaaacgtct gccaaaccac aaaaaaagcg actgtattcc
     1801 agccgctgta agttaagcat cctgcatttt caagcagaac atatgttaca ctataacctt
     1861 aaatccgaaa ggagtttcga tgaacaaaat tccgtgaagc ccccacattg gagttccatc
     1921 tacatgaacc ctgatgcaac cgcccgggag attatgcaaa taagtcggag gggttacttt
     1981 gtagaatccg aatgggccgg gtaagtaaaa acgacctttg tagtatgcgt agttcattcc
     2041 atcaatggca atccaaacgg ctttgcgagg cagatgtctg tatctcacat ttttgtgatg
     2101 atgccaaatg tagtcgttcg ggtgaacgcg cggtggattc acatgtacgt atttgggacg
     2161 attattatgg cttgaatggt atccattccc acggtgccca tcgtttcgac cgtattcatc
     2221 gttatgcttg tcacgaccat gccgttcgtc acgaactcca tctcgcccgt tattaccttt
     2281 tcctcgattg ccattgccgt tttcatatcg accatcgcgc cccttgtcgt tgccatggcc
     2341 tttgccccga ttattatcga acgactgttt ctctgttttt ttcggttctt ttacgctatt
     2401 gttggctaaa cggctcttgg gattgtcttt tttctcaacg cgttcattgc ttgccttggc
     2461 attttctaaa gtacgtcgtt gtgcgtttgc ctctgttgca gtgaaagcaa ctactaatat
     2521 tgcgattgct gttgcgagga ttctggtttt atatgttatt gttttcatag cttttgtatt
     2581 taaatggttt aacaaaagcg ggcccgttga tgaggaaaaa acaaacactg tgccaaaaaa
     2641 gaaaaaagca ggtcaacaga agaaatccaa tcggcaaacg atcgagcgac gtttatgtct
     2701 ttctgtcatt acagagtccg caggcatcta ttatccagcc agttctttat gaaaagtgta
     2761 agactgcgac cagaaatcaa ccgtattgaa tccgtgcaaa acaatttcac ctttgaccaa
     2821 tttttgtaca aaaatttgtt tggtattata gcatcagccg gtactatctc tgttcaacca
     2881 ttgtttaaaa agtgtcacaa tggttgaata tgaggggata gaataaatta ccaaagctat
     2941 at
//
LOCUS       DAOPUW010000198         2941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_34884_length_2941_cov_3.307692, whole genome shotgun sequence.
ACCESSION   DAOPUW010000198 DAOPUW010000000
VERSION     DAOPUW010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34884_length_2941_cov_3.307692"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PKJ43_04310"
     CDS             complement(<1..250)
                     /locus_tag="PKJ43_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71819.1"
                     /translation="MDKELLQAIDELINRGLHEDVNTGDITTNLLIPEGKQTTAVMIA
                     KADGIIAGLQVAEAVFRKLDKDVKFIPSVNDGDKVKKGD"
     gene            complement(252..1826)
                     /gene="nadB"
                     /locus_tag="PKJ43_04315"
     CDS             complement(252..1826)
                     /gene="nadB"
                     /locus_tag="PKJ43_04315"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNZ71820.1"
                     /translation="MKYLEHDYVIIGSGLAGLTAAYYASRFGSVAIITKAGLDVSNSY
                     FAQGGIAAVLHPDDTVEDHINDTLVAGRGLCDYDAVEILVNEGRERVLELIEMGMKFD
                     KENGEYVMGLEGGHSKRRILHASGASTGRDMTRFMVEKVKEIPNITAYEKTKAVELFV
                     KDGRCRGLKAICFEDMSTMCFQSNVVILATGGLSRLFQRSTNPYTATGDGIALAWNAG
                     AKLTDMEFIQFHPTALMIPGQDAFLLSEAIRGEGAYLLDKNGERFMQKIHPMAELAPR
                     DTVASAIYNQMKKDQTQNVYLTLQHLDKNYIKERFSVIYEEILKKGVDITQDLIPIAP
                     AAHYMVGGIRTGLNGETTVPGLWACGEVAATGVMGANRLASNSLLECLVFARRAAFHA
                     ASFVAEPTEKTETTPIVYNYNHVDEFLEIKNEISDIMSNNVCLIRNHEGLTHAVTRLK
                     EIHEAYRNKDDEYNHSKINNIADISFLIARSALIREESRGGHVRADFPETSPEFEMHI
                     SQTRNEEPIFEPVRKK"
     gene            2210..>2941
                     /locus_tag="PKJ43_04320"
     CDS             2210..>2941
                     /locus_tag="PKJ43_04320"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ71821.1"
                     /translation="MGGEQSATSRRNFIKTAATGLAAITILPSNVVAGLGHTPPSSRL
                     SIAAIGVGGVGFKNLNAMNGENIVALCDVDRSYGLKAFRRWPAAVRYTDFRKMLEAEK
                     NIDAVLIAAPDHIHAHAASMAMRLGKHVFVQSPMGHSVQEIRRMAQLSKSYPIVSQVG
                     NTIASSDISRRVAEIIWNGDLGEIREVDVWSSGPEWPQGFLPDTKKTNVPVTLYWDLF
                     LGPAESIPYKDNYTPYGWRAFWSFGS"
BASE COUNT          718 a          614 c          658 g          951 t
ORIGIN      
        1 ggtcgccttt ttttacctta tcgccatcgt ttactgacgg aataaatttc acgtctttat
       61 caagttttct gaacacggcc tcggctacct ggagcccggc aattatgcca tcagctttgg
      121 caatcatgac tgcagttgtt tgctttccct cggggataag caaattggtt gtaatgtctc
      181 ctgtatttac atcttcatgt aatccgcggt taatgagttc atctatcgct tgtaaaagtt
      241 ctttatccat tttatttctt tctgacaggt tcaaaaatag gctcttcgtt tcgtgtttgt
      301 gaaatatgca tttcgaattc ggggcttgtt tccgggaaat cggctcgcac atgaccgccc
      361 ctgctttctt cccggataag ggcagaacgg gcaatcagga aactaatgtc ggcaatgttg
      421 tttatttttg aatggttata ttcatcgtct ttgtttcggt atgcttcgtg aatttctttc
      481 aatcgtgtta cagcatgtgt gagcccttca tggtttcgga tcaaacaaac attgttcgac
      541 atgatgtcag aaatttcatt ttttatttcg aggaattcat caacgtggtt gtagttgtac
      601 acaattggag tagtttcagt cttttcagta ggttcagcaa caaaacttgc ggcatggaaa
      661 gcagctcttc ttgcaaatac gagacattcg agtaatgagt tactggctag ccggttggca
      721 cccataactc cggtagcagc aacttcgccg catgcccaaa gcccggggac agtagtttct
      781 ccgttaagtc cggtccgtat tcctccaacc atgtaatgtg cggcaggagc aatgggtatt
      841 aggtcttgcg taatgtcgac gccctttttt aatatttctt cgtaaataac tgaaaagcgt
      901 tcttttatgt aatttttgtc aaggtgctga agggtgagat agacattctg tgtctggtct
      961 tttttcatct ggttatatat ggcgcttgcc acagtatcgc ggggggcaag ttcggccatt
     1021 ggatgaattt tttgcatgaa ccgttcccca tttttatcga gcaggtaagc gccttctccc
     1081 cgaatagcct cactgagaag gaaggcatct tgcccgggaa tcatcagtgc cgttggatgg
     1141 aattgtataa attccatgtc ggtaagtttt gctcctgcgt tccacgccag ggcaattcca
     1201 tcgccggtgg cagtgtaggg gttggtgctt cgctggaata gtcgggaaag acctcctgtg
     1261 gcaagaatta ccacattgct ttggaagcac attgttgaca tgtcttcgaa gcaaatggct
     1321 tttaaaccac ggcaccttcc gtcttttaca aataattcaa cagcttttgt tttttcgtat
     1381 gcagtaatat tcgggatctc tttaactttt tctaccatga aacgggtcat gtcccgcccg
     1441 gttgaagctc cgctggcatg taaaatcctg cgttttgaat ggcctccctc aagtcccatc
     1501 acatactcgc cgttttcttt atcgaacttc atgcccattt caattaattc aagcacacgt
     1561 tcacggcctt cgttgactaa tatttccaca gcatcatagt cgcaaagacc acgcccggca
     1621 actaatgtgt catttatatg atcttcaacg gtatcatctg ggtggagtac ggcagctatc
     1681 cctccttggg caaaataaga attgctaaca tcgaggccgg cttttgtaat aatggctacg
     1741 ctaccaaacc tggaagcata atacgctgct gtcagtccgg ctaatccgct gccaattatc
     1801 acataatcat gttccaggta tttcactttc ttaaatttta gttgttttga gcaatcattg
     1861 ttctattcga taaacctatg ctctagttcc gctcagtata aaaatggaaa tgtatacatt
     1921 tggatcgaaa agttagtgac tgaatttaaa aaattggtgc aaaattaaga ttattcacaa
     1981 aacgctgatt tttgtgtcac tttttcagtg aaaatacgaa ggttttactt cgttttctat
     2041 ttctcgaagc agggtacaca actacatatt gtgttgagat atacgtgctt ttcagagtct
     2101 ttaaacgtta tattgtatgg ttgcgttgaa tgaattattt ttttgctttt catgccgctt
     2161 tcaaaaggat gaatatttag attaaatttg agaggaaatt ccaagaacta tgggaggaga
     2221 acaatctgcc acttcacggc gcaactttat aaaaactgca gcaaccggtc ttgctgcgat
     2281 tactatttta ccttctaacg ttgttgcggg gctggggcat acgcctcctt cgtcgcgatt
     2341 gtccattgct gcaattggtg ttggaggagt tgggtttaag aacctcaatg ccatgaatgg
     2401 agaaaatatt gttgcgttgt gtgatgttga cagaagctat gggcttaaag catttcggcg
     2461 ctggcctgcg gcagtgcgtt atactgattt tcgtaaaatg ctcgaagctg agaaaaatat
     2521 tgatgccgtt cttattgctg ctcccgatca tattcatgcc catgctgcgt ctatggcgat
     2581 gagactggga aaacatgttt ttgtgcagtc tccgatgggg cattccgtgc aggaaattag
     2641 gcgtatggca caattgtcga aatcgtatcc aattgtaagt caagtgggaa acactattgc
     2701 atcttcagac atctcgcgtc gggttgctga aataatttgg aatggtgatc ttggcgaaat
     2761 acgcgaagtt gatgtttgga gttcgggtcc cgagtggcct caaggttttc ttcccgatac
     2821 gaaaaaaacc aatgtgccgg taactctcta ttgggatctt tttcttggac ctgcagaatc
     2881 tattccgtat aaagataatt atacgcccta cggttggcgg gcgttttggt cctttggttc
     2941 g
//
LOCUS       DAOPUW010000199         2933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_35005_length_2933_cov_3.573662, whole genome shotgun sequence.
ACCESSION   DAOPUW010000199 DAOPUW010000000
VERSION     DAOPUW010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35005_length_2933_cov_3.573662"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PKJ43_04325"
     CDS             <1..783
                     /locus_tag="PKJ43_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ71822.1"
                     /translation="RSYSIKTDILIQQEKYKEAREYLKKSLEIDPNRYSLWEQLVFID
                     NQLLDWPEMTSDSKKAIELFPTQPLLYMLNSIALIQLKQYKEALSSLETGQNYVVDNK
                     KIEAQYEIYRAEAHYNLNNYAEAFTAFDKVLALEPDNYMAMNNYAYYLSVRGEQLEKA
                     ESMSSKVVVANPDNSTYLDTHAWVLFKRKEYRLAKHYMEIALNNGGDVSDVIVEHYGD
                     ILFMLNDIEGAVAQWKKSKELGNPSEILQQKIDSKSYIEEKE"
     gene            786..1091
                     /locus_tag="PKJ43_04330"
     CDS             786..1091
                     /locus_tag="PKJ43_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71823.1"
                     /translation="MNMNRCKIYYLLTLCAITVLFASCSTSRWVALTEIRPMAAQKLM
                     RKVERESPSFNSYGSKKVNIEYTLNNQKNSFSGQFKLKRNECLIASARKFTMTVVKG"
     gene            1088..1642
                     /locus_tag="PKJ43_04335"
     CDS             1088..1642
                     /locus_tag="PKJ43_04335"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HNZ71824.1"
                     /translation="MITPDSIVFINYFDRSFLSGTFDDIKKVSGADVSFNLIEALLTG
                     DISHIIDFEIFDRSFTSSIDSQMYRIDSPVKNIALAGKKNKNVLQQAGFENISVWVDP
                     QKYVVRKISLSDNKQNQKIGISYGNYESIKGDQYPQEVIFDYITPSQKASFTLSLSKS
                     EVNAENDFSFSVPEKYEKLKLNGL"
     gene            1639..2868
                     /locus_tag="PKJ43_04340"
     CDS             1639..2868
                     /locus_tag="PKJ43_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNZ71825.1"
                     /translation="MITRKTGSNIALARLSAIKPLIFIVLFSCCLPLFSGAQTLDELR
                     KRKEKTQKEIEYLNSLINETGENTRATVNKLSMLNQQIELRNNLIIDYNTQLSMLQKS
                     IGENMQTIQSMNDDLATLRARYEAMIKQAFRNRGNYSQLVFLLSSANFNQAYKRMFYI
                     RQMARYRKKQSEEIEMLKASLETQTVELNNKKQEQTEILNQQMAETSKLNLEKEKQAG
                     YQKQLQKKEKELKRKLQEQQRLERKLQEGIERLIAQEVKKSKTTAATPEEKLLSSDFA
                     NNKGRFPWPVQNGVITDRFGVHAHPVMKQLIIENDGIDISTSPGEKARAIFNGTVSRI
                     FSLTGGNLAVIIRHGEYMTVYSNLREVFVKQGDTVNTKQEIGLIFTDTFEDNKTTLKF
                     QIRKETLKLNPEEWISR"
BASE COUNT         1009 a          633 c          609 g          682 t
ORIGIN      
        1 cgcagttaca gcataaaaac cgatatcctc attcaacagg agaaatacaa agaagcccgc
       61 gaatatctta aaaaatcgct cgaaatagat ccgaaccgtt attcgctttg ggaacaactg
      121 gtgtttatcg acaaccaatt gctcgactgg cctgaaatga ccagcgatag caaaaaagcg
      181 atcgagctct tccctaccca accgttactt tatatgttga attctattgc tttaatacag
      241 ctaaaacaat ataaagaggc cctttcgtcg ctcgaaaccg gccaaaacta tgtggtcgac
      301 aacaaaaaaa ttgaagcaca atacgagatt taccgggccg aggctcatta caacctcaac
      361 aattatgctg aagcatttac ggcattcgat aaagtgctgg ccctggaacc cgacaattat
      421 atggcaatga acaattatgc ctactacctg tcggtcaggg gagaacaatt agaaaaagct
      481 gaatcgatga gcagtaaggt cgtggtagcc aatcccgata attcgaccta cctcgatacc
      541 catgcctggg tgcttttcaa acgaaaagaa taccggctgg ccaaacacta catggaaata
      601 gcccttaaca acggtggcga tgtaagcgat gtaattgtag aacactatgg tgacatattg
      661 tttatgctca acgacataga aggagcagta gctcagtgga aaaaatcgaa agagctggga
      721 aatccttcag aaatccttca gcaaaaaatc gattctaaaa gctatatcga agagaaagaa
      781 taagaatgaa tatgaatcgt tgcaaaatat attacctgct taccttatgc gccataacgg
      841 tgctttttgc ctcgtgtagc acaagccgct gggttgcttt gactgaaatc aggcccatgg
      901 cggctcaaaa actgatgcgg aaagtagagc gcgaaagccc ttcgttcaac tcgtacggat
      961 cgaaaaaagt aaacatagaa tataccctga acaaccaaaa aaacagcttt tcggggcaat
     1021 ttaagctgaa acgcaacgaa tgtttaattg cctcggcacg taaatttacc atgaccgtgg
     1081 taaagggatg attacccctg acagcattgt tttcatcaat tatttcgacc gcagtttctt
     1141 gtcgggaacc tttgacgata ttaagaaggt tagtggtgcc gatgtcagtt ttaaccttat
     1201 tgaagccctt ttgaccggag acatcagcca tattattgat tttgaaatat tcgacaggtc
     1261 atttacttcg tccatcgact ctcagatgta ccggatcgac agcccggtta aaaacattgc
     1321 tttggccgga aagaaaaata aaaacgtatt acagcaggct ggtttcgaga atatttcagt
     1381 atgggttgac cctcagaagt atgtagtccg taaaatctct ttgtcggaca ataagcaaaa
     1441 ccaaaaaatt ggaataagct acgggaacta tgaatcgatt aaaggcgacc aatacccgca
     1501 ggaagtcatt ttcgattaca ttaccccctc acaaaaagca tcattcacgc ttagcctttc
     1561 gaagtcggaa gtaaatgctg aaaacgattt tagtttttct gttcctgaaa agtatgaaaa
     1621 gttaaaactg aacggattat gatcacgaga aaaacaggaa gcaacattgc acttgccaga
     1681 ttatcagcaa taaaacctct tatttttatt gtcttgttct cgtgttgcct acccctgttt
     1741 tcgggagccc aaacgcttga tgaactccgg aagcggaaag aaaaaacgca aaaagaaatt
     1801 gaatatctca actcactaat caacgagacc ggagaaaata cccgcgccac agtaaataaa
     1861 ctttcgatgc tgaaccagca gatagagctg cgcaacaacc tgatcatcga ttacaatacc
     1921 cagttaagca tgctccaaaa atcgattggt gaaaacatgc agaccatcca atcgatgaac
     1981 gatgacctgg ccacgctgcg tgcccgctac gaagcgatga ttaaacaggc attcagaaac
     2041 cgcgggaact acagtcagct ggtttttctg ctttcgagcg ccaactttaa ccaagcctac
     2101 aaacggatgt tctacatcag gcaaatggcg cgttaccgca aaaaacaatc ggaagaaatt
     2161 gaaatgctaa aggcttcact ggaaacccaa accgtagaat tgaataataa aaaacaggag
     2221 caaacagaaa tcctgaacca gcaaatggct gagacatcga agcttaacct tgaaaaagaa
     2281 aagcaggcag gttaccaaaa gcaattgcaa aaaaaggaaa aggagctgaa gcgaaaatta
     2341 caggaacagc aacgactgga aagaaaactt caggaaggaa tagaacgcct tattgcccag
     2401 gaagttaaaa aatcgaagac aactgcggcc accccggaag aaaaactatt atcgagcgac
     2461 tttgccaaca ataaaggacg ttttccctgg cctgttcaga acggggtaat aacagaccgt
     2521 tttggtgttc atgcccaccc ggtgatgaaa cagcttatta ttgaaaacga tgggattgat
     2581 ataagcacca gtccgggcga aaaagcacgg gcaattttca atggtacggt aagccgtatt
     2641 ttttccctta caggaggaaa ccttgcggta atcattcgcc atggtgaata catgactgta
     2701 tactctaacc tcagagaagt gtttgttaaa caaggtgata ctgtgaacac aaaacaagaa
     2761 atcggcttaa tattcaccga tactttcgaa gacaataaaa ccaccctgaa atttcagatt
     2821 cgcaaagaaa ccctgaagct gaaccccgaa gaatggattt cgaggtaaaa aggcactaaa
     2881 cgttggcaat tttacggata gaagcctcat cgataaaggt tatcctgcgg cct
//
LOCUS       DAOPUW010000200         2932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_35027_length_2932_cov_3.484880, whole genome shotgun sequence.
ACCESSION   DAOPUW010000200 DAOPUW010000000
VERSION     DAOPUW010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35027_length_2932_cov_3.484880"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..899)
                     /locus_tag="PKJ43_04345"
     CDS             complement(114..899)
                     /locus_tag="PKJ43_04345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ71826.1"
                     /translation="MTNLTKTSLHKIAAMRYEYLESLPRFQDIYLEFMINESMCYTIK
                     ENDIPLGYAIATNDAVLLEFYLFLPYQNHRTRLFATIVEQLKIESILCKSFDSILLDC
                     CLLYNFSYSLIGCLFREHTGRTDRKNSNLTYRFANKIDLPFLLAQNDEVFEPKELLAK
                     NIDDESIVIAGNHESPVVACGFLTQVHPQFNYYDLGIWVDEAYRRKGIASSTMQYMIE
                     LCTEKKGIAICGCDIQNVASQNMLEKLGFISKHKLIEFTTKKN"
     gene            1254..1976
                     /locus_tag="PKJ43_04350"
     CDS             1254..1976
                     /locus_tag="PKJ43_04350"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HNZ71827.1"
                     /translation="MKKSLFIVTLIFASVVVAEASSLFWGQIVKGNGKVTKENRSVNS
                     FQGVSASAGINVYLFAGDEEKVIVETDENLQDCLITKVKGNTLECYFDCNIRKSTKTN
                     VYVTYRKINHIHASSGSNVTGETPIKAQELEIKANSAADIKAEVYANTIECYVASGAD
                     VELDGKASHFKGRADSGADLKAQDLTAETADVNSSSGANIQIAVTKSIDADASSGGNV
                     EYFGKPQNENVHESSGGDVRRR"
     gene            complement(2069..>2932)
                     /locus_tag="PKJ43_04355"
     CDS             complement(2069..>2932)
                     /locus_tag="PKJ43_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71828.1"
                     /translation="KQRSWPKQDSLYTRLYRASMENELFLKQLINESDADKECREIAA
                     LTLDKLLIESKNNPYAQDSINEKADNFLNNSESKRYNDYIKKHIRYKLIAKNWSASVE
                     FFSGYGMLTGELSNTYRNFLPIGVGIGIGYKNADLSIRALNGNHKTNKDLTYSTGIYT
                     KGSKVRIFIPEITLGYSILNNNYLKISPFGGIGLSDIRATTDQTDKVPELKELSTGYP
                     AMYVAGLNIEVKFGPKKMPEFMPKTNYSYLNVRYSYGMPQFEKKYAGMHGNFHYITIG
                     HGSMSRGTHRQ"
BASE COUNT          806 a          515 c          621 g          990 t
ORIGIN      
        1 gtttttccgc tataatgtat atttctctgt tgggtataaa acgtaataca atggttttcg
       61 ttgctttatt tcagatcgat taagtgaaat gtatgtttct taaatttaat gtgttaattc
      121 ttttttgtcg tgaattcaat taatttgtgt ttgctgatga atcctaattt ttcgagcatg
      181 ttttgtgagg caacgttctg tatatcgcag ccacaaatgg caataccttt cttttcggtg
      241 cataactcta tcatatactg catggtactg ctggcaatgc cttttcgccg ataagcttca
      301 tcgacccaaa tccctaaatc gtaatagttg aattgagggt ggacttgtgt aagaaaacca
      361 caggctacaa ccggagattc gtgatttcca gctataacaa tcgactcatc atcaatgttt
      421 ttggctaaaa gctctttggg ctcaaaaact tcatcatttt gggctaaaag aaaaggtaag
      481 tcgattttgt ttgcaaatcg gtaggtaagg ttagagtttt ttctgtccgt tcgcccagta
      541 tgttcacgaa acaggcaacc aatgagtgaa tatgagaagt tgtataataa acagcagtct
      601 aatagaatag agtcgaacga tttgcacaga atgctttcaa ttttcaattg ttcaacaatt
      661 gtggcgaata gacgggtgcg atggttttga tagggcagaa ataggtaaaa ttcaagtaaa
      721 acagcgtcgt tcgttgcaat ggcataaccc aacggaatat cattctcctt aatagtataa
      781 cacattgatt cgttgatcat aaattctaaa taaatgtctt ggaaacgtgg tagcgattcc
      841 agatattcat agcgcatggc agcaattttg tgaaggcttg ttttcgttaa gtttgtcata
      901 attattctat gtgttgtttt ttctgaaata tttgattttt cgatcgataa aacggcaata
      961 aatatacgtg atatgatcgt tttgggaaaa tagttctgtt ttcttttcta ttggttattt
     1021 caccccttgc cgtgaatgtc ttttttcggc attcaatttt cttattttac cgataaaacc
     1081 aggccgttgg cattttttta cttcgtgctg taacttcccg ctcatacttt cgtcatactt
     1141 tttaaaagca acaacctgcc cgctgggagc tttttaagaa agtttcgttg ttgtaaaatc
     1201 tatagtctat acactgataa atattgtatt tgtttaataa taagaataat accatgaaaa
     1261 aatcattgtt tatcgttaca cttatttttg cttcggttgt tgtggccgag gcatcttctt
     1321 tgttctgggg ccaaattgta aaggggaatg gcaaggtgac taaagaaaac cgttcagtta
     1381 attcgttcca aggggtttca gcttctgccg gaattaatgt gtatttgttt gccggtgatg
     1441 aagaaaaggt aattgttgaa accgacgaaa acctgcaaga ttgcctgata accaaggtga
     1501 aaggtaatac actcgaatgt tattttgatt gcaatattcg taaatcaacg aaaactaatg
     1561 tgtatgttac gtatcgtaaa atcaatcata tacatgcttc gtcaggatcg aatgtcacag
     1621 gcgaaacacc tataaaagcc caggaacttg aaataaaggc caatagcgct gccgatataa
     1681 aagctgaagt ttatgccaat acgattgaat gttatgtggc tagtggtgct gatgtggagc
     1741 ttgacgggaa agccagtcat tttaaaggga gagctgattc gggagccgat ctgaaagcac
     1801 aagaccttac tgctgagact gcggatgtga actcgtcgag tggagcaaat attcagattg
     1861 ctgtaacaaa aagtattgat gccgacgcta gtagcggtgg taatgttgag tattttggga
     1921 agccacaaaa cgaaaacgtg cacgaatcga gtggaggaga tgttcgccgc cggtaataac
     1981 atagtaaggg cgattatttt cgcccttttt tgtttttatg cttaaaaaat atccaatcaa
     2041 gaactcaggt tgtcggtctt caatcttctt attgtctgtg ggttcctctc gacatggagc
     2101 catggccaat cgtgatatag tggaagttgc catgcatgcc ggcatacttc ttttcaaatt
     2161 gaggcatacc ataactatat ctgacattca ggtaggaata gttagttttg ggcataaatt
     2221 ctggcatctt ctttggaccg aatttcactt caatattgag gccagcaaca tacattgcag
     2281 ggtatcctgt agataattct ttcaattcgg gaactttatc ggtctggtct gtagttgccc
     2341 taatgtcact tagtccgatg cccccgaagg gtgatatttt caggtagtta ttgttcagta
     2401 tgctgtatcc taaggtaatt tctggtatga aaatcctaac ttttgagcct ttcgtgtaaa
     2461 ttcccgtaga gtaggtgagg tctttatttg ttttatggtt cccgtttaat gccctaatcg
     2521 atagatctgc gtttttgtag ccaatgccaa tacctacgcc aatagggaga aagttgcggt
     2581 aggtgttgct caattccccg gttaacattc cgtatcctga gaagaactct accgatgcgc
     2641 tccagttctt ggcaatcaac ttgtaacgga tgtgtttttt gatataatcg ttataacgtt
     2701 ttgattcaga attgttgagg aaattatcgg ctttttcgtt gatcgaatct tgagcataag
     2761 ggttattctt gctttctatc agtaacttat cgagcgtgag tgctgcaatc tcccggcatt
     2821 ctttgtctgc atccgattca ttgatcagtt gtttcaaaaa taattcattt tccatcgatg
     2881 cccggtaaag tctggtgtac agtgagtctt gctttggcca gcttctttgt tt
//
LOCUS       DAOPUW010000201         2929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_35076_length_2929_cov_3.231385, whole genome shotgun sequence.
ACCESSION   DAOPUW010000201 DAOPUW010000000
VERSION     DAOPUW010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35076_length_2929_cov_3.231385"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..1182
                     /locus_tag="PKJ43_04360"
     CDS             223..1182
                     /locus_tag="PKJ43_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71829.1"
                     /translation="MRKIISIVALLLFFSLSLFSQNAGLLRDFHKNWKVSAMGGASYL
                     AFELKKDFMRATMDMNSTPNLAFSVTMNKRFNHHFEVGLEYEKSYFSGFKNNSSSVNW
                     LVYDPQFNTDGKKFIPNALYYDTDISSFYLNFYYCFLNMYSMRYSLLNMNFFLKGGLG
                     FSSIGVAMGYKNPEDYIASNLLDPIYVKGQGRHPWRDSYGTLHLGLGINYYFSPRWSL
                     VLDGTLLFVSSDYLDGVHNFDIKKLPDEQVEMIRIGVFDFVGQLKVGVTYHFDLYKGR
                     RWTGVSPFGKKRNVYKNDFFIDKKYNRVRKPRDTFYKPSYRRR"
     gene            complement(1198..>2929)
                     /gene="cysC"
                     /locus_tag="PKJ43_04365"
     CDS             complement(1198..>2929)
                     /gene="cysC"
                     /locus_tag="PKJ43_04365"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNZ71830.1"
                     /translation="AGDEIDYALLLDGLKAEREQGITIDVAYRYFSTNKRKFIIADCP
                     GHEQYTRNMVTGASTANLAIILIDATKGVITQTRRHTYLVSLLGIKHVVLAVNKMDLV
                     DFRRERFDEICKDYKAFVTQLNIPDVEFIPLSALKGDNVVSSSQNMLWYHGKSLLEFI
                     ETVHVSSDRNFEDLRYPVQYVNRPDKDFRGFAAKVASGVIRKNDLIMVLPSRKTSVVQ
                     SITTYDGELDHAFPPQSVTITLADEIDISRGEMIVHPDNLPHIERHFEAMLVWMDETP
                     MNPSVQYYIKHTTNTTKARIDSIRYRVDVNTLQKSDIGFLSLNEIGRAIITTVKPLFF
                     DPYTLNHNTGSFVLIDPVSHNTCAVGMIIEKLSSNELSSKISHHEIVEKIDNGQCLIS
                     PADRLKRFNHNGFTIWITGLHGSGKNHLAYLLEKELFSLGANVVLLDGSTVRSGLSRE
                     LDFSNNDRAEHLRRVAHIARLLNDQGIITICSFISPNNDTRNIVKEIIGGQRVVTIFM
                     NTDLEICKKNKPLLYQLADEGKLHDLPGIDTPYENPENANLVLVHNTDFSNIEKVIAL
                     LSEKKLFPAS"
BASE COUNT          759 a          559 c          657 g          954 t
ORIGIN      
        1 aatgtctaca acagttacgg taatgtttgg gcatttaagt gcaataaccg ccatggttgg
       61 gccgccaaca tagccggcac ctatgcaaca tatgcgtgaa atcttattgg gtatattcat
      121 gataaacagg ttgttgtgtt tatgtttctg gttagttaaa gtacgatttt atttttttat
      181 aagtagggta atgtttccta ataatgttat ttttgcgtgt ttatgagaaa aatcatttcc
      241 atagttgccc ttctgttgtt tttttcgctc agtttgtttt ctcaaaacgc tgggctcctt
      301 cgcgatttcc ataaaaactg gaaggtatcg gctatggggg gtgccagtta tttggctttc
      361 gaactgaaga aagatttcat gcgggctacg atggacatga attcaacgcc aaacttagcg
      421 ttttctgtca cgatgaacaa acgttttaat catcatttcg aagtggggtt ggaatatgaa
      481 aagagttatt tctctggctt caaaaacaat tcttcgagtg taaactggct tgtgtatgat
      541 cctcaattta atactgatgg gaaaaaattt atacccaatg ctctctatta cgacactgat
      601 atctcatcgt tctatctaaa tttttattac tgtttcttga atatgtattc gatgaggtat
      661 agcttgttga atatgaattt ttttctcaaa ggtggcttgg gcttctcatc aattggtgtt
      721 gctatggggt ataaaaaccc agaagattat atcgcatcaa atttgctgga tccaatttac
      781 gtaaaagggc aaggccggca tccgtggcgt gactcgtatg gtacattgca tttggggctt
      841 gggattaatt attatttttc accccgttgg tcattggttc ttgatggaac attgctgttt
      901 gtaagttccg attatcttga tggcgttcac aatttcgata ttaagaaatt gcctgatgag
      961 caggttgaaa tgattcgtat tggggtcttc gattttgtgg ggcagctcaa agttggcgtt
     1021 acctaccatt tcgacttata taagggacga aggtggactg gtgtgtcgcc ttttggaaaa
     1081 aagcgtaacg tgtacaaaaa tgatttcttc atcgataaga aatataatcg cgttagaaaa
     1141 ccccgtgaca cattttacaa gcctagctat cgtcggagat aattttttat taacttctta
     1201 gctggccggg aagagttttt tctcacttaa gagtgcaatc actttttcaa tatttgagaa
     1261 atcggtatta tgcaccaaaa ccaagttcgc attttcaggg ttttcgtaag gagtatcgat
     1321 cccgggcaaa tcgtgtagtt ttccttcatc ggccaactgg tataacaagg gtttattctt
     1381 tttgcagatt tccaggtcag tattcatgaa aattgtcaca acacgttgtc ccccaattat
     1441 ttcttttact atgtttcgtg tgtcgttgtt tggcgagatg aacgaacaaa tggtaattat
     1501 tccctgatcg ttcagtaaac gggcaatgtg agccactctt ctgaggtgtt ctgcccggtc
     1561 gttgttcgag aaatcgagct cgcgcgataa acctgaccgc acagtacttc cgtcgagcaa
     1621 taccacattg gctccaaggc tgaagagttc tttctcgaga agataggcaa gatgattttt
     1681 acccgaacca tggagcccgg taatccaaat ggtaaagcca ttgtggttga aacgttttaa
     1741 ccgatcggct ggagaaatga gacattgccc attatctatt ttttcaacta tttcgtggtg
     1801 tgatattttg ctggagagtt cgttgctgct gagtttttct ataatcatac ccaccgcaca
     1861 ggtattatgg ctgaccgggt cgattagcac aaacgaacct gtattgtggt taagagtgta
     1921 cggatcgaaa aacaagggtt taactgtagt gattatggct cggcctatct cgttgagcga
     1981 caggaatcca atatccgatt tctgtaatgt gttcacatca actctgtacc ttatcgaatc
     2041 aattcgggct ttggtcgtgt ttgtagtgtg ctttatataa tattgtaccg atgggttcat
     2101 gggggtttcg tccatccaca ccagcatagc ttcaaaatgt cgttcaatat gtggcaagtt
     2161 gtcgggatga acaatcattt ctccccttga gatgtcgatt tcatcagcca gtgtaatggt
     2221 aaccgactgt ggggggaagg catggtccaa ttccccatcg tatgtagtaa ttgattgtac
     2281 aaccgatgtt tttctggacg gaagtaccat gatgagatcg ttcttgcgaa taacacccga
     2341 ggctactttg gccgcaaatc ccctaaaatc tttatcggga cggtttacat attgtacagg
     2401 atagcgcaga tcttcaaaat ttctgtcgct actaacatgc actgtttcaa taaactccaa
     2461 taagcttttc ccatggtacc acagcatgtt ctgcgaggaa gaaacaacat tatcgccttt
     2521 aagtgccgat aaagggatga attccacatc gggaatgttc agttgggtta caaatgcttt
     2581 atagtctttg cagatttcgt cgaagcgttc acgtctgaaa tctacaagat ccatcttatt
     2641 tactgccaga actacatgct tgatgcccaa taacgataca aggtatgtgt ggcgccgcgt
     2701 ttgggtgatt acccctttgg ttgcatcgat aaggataatg gcaaggtttg cggttgaggc
     2761 tcccgtaacc atgtttcggg tatattgttc gtggccgggg caatcggcaa tgataaattt
     2821 tcttttgttt gtactgaagt accgataagc aacatctatg gtaatgcctt gttcgcgttc
     2881 ggctttcaaa ccgtcgagca gaagggcata gtcaatttca tcgcctgcg
//
LOCUS       DAOPUW010000202         2917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_35250_length_2917_cov_3.121593, whole genome shotgun sequence.
ACCESSION   DAOPUW010000202 DAOPUW010000000
VERSION     DAOPUW010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35250_length_2917_cov_3.121593"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..1064
                     /locus_tag="PKJ43_04370"
     CDS             246..1064
                     /locus_tag="PKJ43_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71831.1"
                     /translation="MRTLFSNAVLIILLLGNVNSLRASEPDTSKYPKQLQVNAGYYNL
                     GSIDEIYSYNLYSGSDFCYGLKYTYGKRPVRQIISIRFSTINRRPHSLILAENLVASD
                     ARERILNSFVADAFYTCQFPIELKGADYLRFWVTGTWLTTFNITLNSYGTPELVQSGL
                     APGAFLETNFKRHNFHCQLSVPIISWTLRNSYSQSMTQNFEKINTFNFIRSNDQLQFP
                     NTLLAVYSNIGYVFSLSSRFNIECEYDFRYIYNSSPRKLESVSGIYSFGLTYKF"
     gene            1070..2179
                     /locus_tag="PKJ43_04375"
     CDS             1070..2179
                     /locus_tag="PKJ43_04375"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNZ71832.1"
                     /translation="MKKNTFLWIAILLSLAVSSCEESLIKEEFENTPVDNYNAFWFEF
                     DRFYGAFEAKNINWDSLKTVYSGNLTDSSTDMELFTAMSGLLDELNDGHADLLAPGVG
                     FFRSWNRRNKSFFADNKSTDNSGIVMMQNIIRSNYLKNKYKAGDYSGWKFFYGTITYE
                     THRVGYICIPTFGNDDYPNDFIQQAVDSFNTLDAVIIDLRFNGGGKTEAFVTSLNSFS
                     SERKLYMKSKFRNGSGQSDFTQLDDHYTNPHPDCLKNKPVAILMNSFSASSSDHFILG
                     MKSQSNVITVGDTTCGAFSSVLERILPNGWKFRLGAQVVYGPDGKLLTDSNGKYLEGV
                     GIAPDIYIQEQFSAIRKGKDLPLEAALKNLEGRLN"
     gene            2357..>2917
                     /locus_tag="PKJ43_04380"
     CDS             2357..>2917
                     /locus_tag="PKJ43_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71833.1"
                     /translation="MNKLIFLMIAVFLLSTYNADAQLDLGKLKNKVKDAVNNEKSSGT
                     SSSSHSSSGESTEKKITKAFEDPYEPHKDNGKSTDAIHANHLKEIVFSSTPIESTIKS
                     FNIQGNATEAMLAKSFSLTDDIYMMAYFEQSFYNQMMNDKTALPENTYYTPRIWFEVN
                     GKISGKKGSNKFEKSMGKYDFLEWTNL"
BASE COUNT          911 a          523 c          584 g          899 t
ORIGIN      
        1 ccacgagttt taatttcttg attgtcagta tccatcaggc agtggtaaca agtattgatt
       61 tcaccgataa atagaatctg gtaaagaagt tgttgtattc aataacggct aattatatat
      121 tatggtgtta cgttgattgc cagaaagcaa ttagttgaga aaaaatgaaa aggttgcaac
      181 ttattatcaa gaatattgac tttattgtat actcaacatg aattcaataa tgtgaaaacc
      241 aacaaatgag aactttattt tctaatgcag tcttgattat tttactgctt ggcaatgtta
      301 attcattgcg tgcctccgaa cccgatacct cgaaatatcc caaacagtta caggtaaatg
      361 ctggctatta taatcttggc tcaattgatg aaatatactc gtacaatctt tattcaggat
      421 ctgatttttg ttatgggttg aaatatacct acgggaaaag accggttcgg caaattattt
      481 caatccgttt ttcgacgatt aatcgccggc cgcattcgtt aatactggca gaaaaccttg
      541 tggccagcga tgcccgtgaa cggatcttga atagtttcgt tgctgatgcc ttctatacct
      601 gtcaatttcc aatcgagttg aaaggcgctg attatcttag gttttgggtt acaggcacat
      661 ggttgaccac gtttaatatt actttaaaca gttatggaac tcctgaattg gtgcaaagcg
      721 gtttagcccc gggcgctttt ctcgaaacca acttcaaaag gcataacttt cattgccagt
      781 tgtcggttcc aataatttcg tggactcttc ggaacagtta ttctcagtcg atgacacaga
      841 attttgaaaa aatcaacact ttcaatttta tacgctcgaa cgatcagttg cagtttccca
      901 acactctttt agcggtgtac agcaatattg gatatgtttt ttcgctgtca agtcgattta
      961 atattgagtg cgaatacgat ttcaggtata tctataattc ttcgccacgg aaattggaat
     1021 cggtttcggg aatttactcg tttggtttaa cctataaatt ttgaatgcga tgaagaagaa
     1081 tacatttttg tggattgcaa ttcttttatc gctcgctgtt tcaagttgcg aagagtcgct
     1141 aattaaagag gagtttgaaa atacccctgt ggataattat aacgctttct ggtttgaatt
     1201 cgaccggttt tacggtgctt ttgaagcaaa aaatatcaac tgggattccc tcaaaacggt
     1261 atattcagga aatttaaccg acagttcaac ggatatggaa ttgtttacgg ccatgtcggg
     1321 tcttttagat gaactcaacg acgggcatgc cgatttgcta gctcccggtg tcggattttt
     1381 caggtcgtgg aaccgtagga acaaatcctt ttttgcggat aataaaagta ccgataattc
     1441 gggtatcgtg atgatgcaaa atataatcag aagtaactac ctgaaaaata aatacaaagc
     1501 aggcgattat tccgggtgga aattcttcta tggaacgatt acctatgaaa cccatcgcgt
     1561 tggatatatc tgtatcccaa catttggtaa cgacgattac ccgaacgatt tcattcagca
     1621 ggcagttgat tcatttaaca cgttggatgc tgtaattatc gatttgcggt ttaacggagg
     1681 tggcaaaacc gaagcctttg ttacatcgtt aaacagtttt tcgtcggaaa ggaaactcta
     1741 tatgaaatcg aaattcagaa atggttcggg gcaatcagat ttcactcagt tagacgacca
     1801 ttataccaat ccacaccctg attgccttaa gaataagcct gttgccatac tgatgaacag
     1861 tttttcggca agttcatccg atcattttat tcttggaatg aaatcgcagt ccaatgttat
     1921 tacggttgga gataccactt gtggcgcttt ttcatcggtg cttgagcgca ttttgcccaa
     1981 tggctggaaa ttcaggcttg gtgcgcaggt tgtatatggc cctgatggaa aattacttac
     2041 cgattcaaat ggtaaatatt tagaaggggt tggaattgct cccgatattt atattcaaga
     2101 acagttttct gcaataagaa aagggaaaga tcttccgcta gaagctgcgt tgaagaattt
     2161 ggaaggaagg ctgaattaga aagtgataaa gttgattttt ttgtcaaact aatttaatgc
     2221 cgctcaaaac cttttacaga agtgaggcta ccattcagtg gcaaaatttt accattcagt
     2281 actatttcaa tttcaacact aacgtagttt gttcaatttt gagcattcaa aaaatgctaa
     2341 tttaaaactc agaattatga ataaactaat ctttttaatg atagcagtat tcttgctgtc
     2401 gacctacaat gctgacgcac agctcgattt gggcaaactt aaaaataagg tgaaagatgc
     2461 ggtaaacaac gaaaaatcga gtggcacatc gtcttcatcg cattcgtcaa gtggagaaag
     2521 caccgaaaag aaaataacca aagcatttga agatccctac gagcctcaca aggacaatgg
     2581 taaaagcact gatgctattc atgccaacca tcttaaagaa atagtatttt caagtactcc
     2641 aatcgagtcg accataaaat cgttcaacat tcaaggaaat gctaccgaag ctatgttggc
     2701 aaagtcgttt tctctaaccg acgatattta tatgatggct tattttgaac aatcgtttta
     2761 taatcaaatg atgaacgaca aaacagcatt gcctgaaaat acttattata ctcctagaat
     2821 ttggtttgaa gtaaatggaa aaataagcgg aaagaaagga agcaacaagt ttgaaaaaag
     2881 catgggcaaa tatgactttc tggagtggac taactta
//
LOCUS       DAOPUW010000203         2903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_35500_length_2903_cov_2.917837, whole genome shotgun sequence.
ACCESSION   DAOPUW010000203 DAOPUW010000000
VERSION     DAOPUW010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35500_length_2903_cov_2.917837"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PKJ43_04385"
     CDS             complement(<1..187)
                     /locus_tag="PKJ43_04385"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNZ71834.1"
                     /translation="MKLLQSFGYAFKGLFAAVQSELNMKIHLLAVIIVVFAGFYFKVT
                     STEWALVLLCMALVIALE"
     gene            complement(197..1240)
                     /locus_tag="PKJ43_04390"
     CDS             complement(197..1240)
                     /locus_tag="PKJ43_04390"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ71835.1"
                     /translation="MPAKRKIIVAPLNWGLGHATRCVPIIHLLIEQGFEVLLASDGDA
                     LQLLQLEFPELTSFELPSYNIEYGHHFAFSVLKRVPHILSTIKRERQAIETIAKNTGA
                     EIIISDNRYGCYTSFTKNIFVCHQHYLLVPVFSDLANSLHRKLLRKFEQHWIPDMEEP
                     KAKFAGKLSTSSLPNVHYIGPLSRFQKMETAKKYDCIAVISGPEPQRSYFEAIIKEQF
                     SASALKTLLVCGKATEASTEKHIGNCTIVPFLNAVQLNQAICASDVVICRSGYSSVMD
                     LATLEKKVIFIPTPGQTEQIYLAEYFQQKNIAPCYKQSNFELNSALMEVKKYSGFHSF
                     EGKNNLRTIIAQL"
     gene            1422..1688
                     /locus_tag="PKJ43_04395"
     CDS             1422..1688
                     /locus_tag="PKJ43_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HNZ71836.1"
                     /translation="MDYIGPQMKRLTQLKKGEFAFVVDLRDPIVCLEMLDNGFFPGEK
                     VDVLENMPDANHILVAMNNKHYRIIKNKANSIITNVVSYDIHLN"
     gene            1745..1921
                     /locus_tag="PKJ43_04400"
     CDS             1745..1921
                     /locus_tag="PKJ43_04400"
                     /inference="COORDINATES: protein motif:HMM:NF028377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HNZ71837.1"
                     /translation="MGRGDKKTEKGKRFKGSFGNARPSKVEKTVAPKAAKTEDKPKKA
                     AAKKAPAKKKATEE"
     gene            2031..>2903
                     /locus_tag="PKJ43_04405"
     CDS             2031..>2903
                     /locus_tag="PKJ43_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNZ71838.1"
                     /translation="MISPIYILLFIGFYFCMLLLISWITSRNADEASYFLGNKQSKWY
                     LVAFGMIGDSLSGVTFISVPGLVANDKFSYLQVVLGYVVGYFIIGKILLPLFYERNLT
                     SIYTYLKTRFGVYSERTGSFYFLISRLLGAGARLYLAINVLQLFVFDGLHIPFALSAS
                     IVIILILMYTIRGGIKTLVWTDVFQSSFLLLAVIFSIVAIGNQLNLSFGGIVQAVVDS
                     PNTQIFYWDWMPKNFFFKQFIGGALIALTMTGLDQNMMQKNLSCRSLKEAQTNIYTFS
                     FIQLGVNVLFLSLGA"
BASE COUNT          847 a          554 c          578 g          924 t
ORIGIN      
        1 attccaacgc aatcaccaat gccatacaaa gcaacaccag agcccattcc gtacttgtaa
       61 ctttgaaata gaaacctgca aatacaacaa taattacggc taataaatgg atcttcatat
      121 ttaactccga ttgaactgct gcaaatagtc ctttaaaagc gtaaccgaat gattgtagaa
      181 gtttcatact attcttttat aactgtgcaa tgatagttcg aagattgttt ttcccttcga
      241 aagaatgaaa tccactgtat tttttgactt ccattaacgc tgagttgagt tcaaaattag
      301 attgtttgta gcagggtgca atgttttttt gttgaaagta ttccgctaaa tatatctgct
      361 ctgtttgtcc gggtgtggga atgaaaatca ctttcttttc caatgttgct aaatccataa
      421 ctgagctata gccggagcgg caaattacca catcagaagc gcaaatggct tgatttaact
      481 gaactgcgtt gaggaaggga acaatcgtgc aattcccaat atgcttttca gtcgatgctt
      541 ctgtggcttt tccgcaaacc aaaagtgttt taagtgctga ggctgaaaat tgttctttaa
      601 taatggcttc aaaataactg cgttgcggct caggtcccga aatcactgca atgcaatcgt
      661 atttttttgc tgtttccatt ttttgaaaac ggctcaacgg ccctatatag tgtacgttgg
      721 gcaagctcga tgtacttaac ttaccggcaa atttcgcttt cggctcttcc atatccggaa
      781 tccaatgttg ctcaaatttg cgaagtaact tgcgatgtaa gctgttcgct aggtctgaaa
      841 acacaggcac cagtagataa tgttggtgac aaacgaaaat gtttttggtg aatgaagtat
      901 aacaaccgta acgattatcg ctgatgatga tttctgcacc agtgtttttg gcaatggttt
      961 cgatggcttg acgctctctt ttaatcgtac ttaatatgtg cggaactcgc ttcagcacac
     1021 tgaaagcaaa atgatgtcca tactcgatat tatatgaagg gagttcaaaa gaggttaatt
     1081 cagggaattc caactgaagt aactgcaacg catcgccatc tgaagccaaa agcacttcaa
     1141 atccttgttc aatcaacaaa tgaattatcg gcacacatcg ggtagcatga cctaatcccc
     1201 agttgagcgg tgcaactatt atttttcttt tcgctggcaa tacttatttt cgattaaatt
     1261 cgtttattaa ttatgaataa aaatagtctt cgtaaaataa tgatagcaat tatttgcttg
     1321 atggcgcttg cccaattacc gtcatgtgcc tcgcaaaagt gcgattgccc taagtttggt
     1381 cgacaacatt taccgcatta ggccttgaca agagcaactt tatggactat attggcccac
     1441 agatgaaacg actaacccaa cttaaaaaag gtgaatttgc ctttgtagta gatcttagag
     1501 accccatagt atgcctagaa atgcttgaca acgggttctt tccgggagaa aaggtcgatg
     1561 tattggaaaa catgcccgat gccaaccata tcttggtggc gatgaacaat aaacattatc
     1621 ggattattaa aaataaagcc aattctatca tcacaaatgt ggtgagttac gacattcacc
     1681 tgaactaggg tttggcaagg cgcatttttt ttgtacgttt gtcgcactta aaattaaaac
     1741 aatcatggga agaggagata aaaaaaccga aaaaggaaaa cgtttcaaag gttcttttgg
     1801 taatgcaaga ccaagtaaag tagagaaaac tgttgcccct aaagcagcaa aaacggaaga
     1861 taaaccaaag aaagcagcag ctaaaaaagc tccagctaaa aagaaagcaa ctgaagaata
     1921 atttttcaag ctctaacttt ttaaagcctc accttgttga ggcttttctg ttttgtgtac
     1981 tatttcttcg taaatagtat attgcaattc ataaaaagta accgcattca atgatttcac
     2041 ctatctatat cctactattt attgggtttt acttttgtat gctattgctg atttcgtgga
     2101 ttacttcccg aaatgccgat gaagccagct attttctcgg caacaaacaa tcgaaatggt
     2161 atttggtggc tttcggtatg attggcgatt ctttatcggg tgttactttt atttcggtac
     2221 ccggcttagt ggccaacgat aaattcagtt acctgcaagt ggtgctgggt tatgtggtag
     2281 gctatttcat tatcgggaaa atcttgctac cgctttttta cgaacgcaat ctgacatcca
     2341 tctatacgta tctgaaaacc agatttggcg tgtattccga acgaaccggt tcgttttatt
     2401 ttttgatttc ccgattgttg ggagccggag cacgtttata tttggccatt aatgtgttgc
     2461 agcttttcgt atttgacgga ttgcatatac cattcgccct ttcggcatct attgttatca
     2521 tacttatttt aatgtacacc attcgcggag gtattaaaac cttggtgtgg acggatgtgt
     2581 ttcaatcttc attcctatta ttagctgtta tattttcgat tgtagccatc ggaaatcaat
     2641 tgaacttgtc gtttggtggc atcgttcagg cggtagtaga ttcacctaat acacaaattt
     2701 tctattggga ttggatgccg aaaaacttct tctttaagca gtttatcgga ggtgcgctta
     2761 tcgcacttac tatgactggt ctcgaccaaa atatgatgca gaagaatttg agttgccgtt
     2821 cgttgaaaga agcacaaacc aatatttaca ctttcagttt cattcagtta ggcgtgaatg
     2881 tgctgttttt gtcgttgggt gca
//
LOCUS       DAOPUW010000204         2893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_35658_length_2893_cov_2.574700, whole genome shotgun sequence.
ACCESSION   DAOPUW010000204 DAOPUW010000000
VERSION     DAOPUW010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35658_length_2893_cov_2.574700"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..223)
                     /locus_tag="PKJ43_04410"
     CDS             complement(14..223)
                     /locus_tag="PKJ43_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNZ71839.1"
                     /translation="MDKLTTTKQALNQLFEVGNLATIPSIFDENYVAHAGDKTYNGHK
                     FIEQFVKQIRNAIPDINISKLEKAG"
     gene            complement(320..1078)
                     /locus_tag="PKJ43_04415"
     CDS             complement(320..1078)
                     /locus_tag="PKJ43_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNZ71840.1"
                     /translation="MNVLIVEDEIYAAQGLEKMLRTIEPGLNVLRVIGSVEETIEYLL
                     ENPTIDLIFMDIQLSDGISFEIFSETKVEYPVIFTTSYDEYAIKAFQVNSVDYLLKPI
                     EKAALQKSIDKYKKLFATPTQNKQIEQMLAQISFVKKEYKSRLLVKTAKGMLTLPIEE
                     VAYIYIDSQMVFVKTTSNQRFALEKTLDELEKQLDPSKFFRLNRQFIVSVKAINAIHN
                     YFNNTLKIELKPPIDREIIVSRYTVKSFKDWLDS"
     gene            complement(1090..>2893)
                     /locus_tag="PKJ43_04420"
     CDS             complement(1090..>2893)
                     /locus_tag="PKJ43_04420"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ71841.1"
                     /translation="GMTYYNIDDLDKALENVTKSYKIDSISNNKKGIARAYNNLGLIY
                     DQKGDYTKCLEFYNKAYPILIELGEKSQLSRLLTNIGIIYTYQKDYDKSLENYNKSIS
                     IKKEIGDNIGIANIYNNIGVIFSRQKQLDSMLHYMQLALEIYTNLDSKRDIAISFGNL
                     GSVYRSMGNYGEALKYIEKAISLRLEMSDIKGAGRSYTSKGYLYSDKNNLPLAMEAFK
                     KALECGEKSGDERLKMESYLNLSTTYEKLNNHKQALTFYQKYHASSDTIYSHQKQKQL
                     LELQTKYETIAKDNEIKLLNNTNEINTLNLEKSKLQIKQQRTVISIALLMLLLVAVFT
                     IVYYRLFKKNQNANRILNEKQLKIEKQNKEILTQHNLLEQLNIELQLQKEKVMNQRDQ
                     IEAELKRTLLSSEILQRENIQFKFEALKNQLNPHFLFNTFSTLIQLIPANPELAEEYT
                     RNLSNVYRYILMGKDKELVQLREEMDFVNAYMFLIAIRFNDSVKLNIDIEQPKYDHYL
                     PLLSLQLLIENAVKHNIISDRKPLIISIKTRGLQLVVENNLQKKSSIENSTKIGLQNI
                     ITRYQLISDQPVIIEQSESHFTVKLPLLNENSYL"
BASE COUNT          727 a          483 c          612 g         1071 t
ORIGIN      
        1 actaaacaag cacttaacca gctttttcaa gtttgctaat gttgatgtcg ggtatggcat
       61 tcctaatttg ctttacaaat tgctcaatga atttatgccc gttataagtt ttatcaccag
      121 cgtgggctac atagttttca tcaaaaattg aagggatagt tgccaagtta ccaacttcaa
      181 aaagctggtt aagtgcttgt ttagtggtgg ttagcttatc cataattgta atttgaaatt
      241 tcagtgaaat gtacggcaca aaattgtacg ttacatcaac tttttggaat ttcttatgaa
      301 tttttttagg atttcgaagt tacgagtcga gccaatcttt aaaactttta acggtatagc
      361 ggctaacaat tatttctcgg tcaatagggg gtttcagctc aattttaagc gtgttgttga
      421 agtagttgtg tatggcgtta atggccttca ccgaaacaat aaattgcctg tttaacctga
      481 aaaatttcga agggtcgagc tgcttttcca attcatcgag ggttttttcc agcgcaaacc
      541 gttggttgga tgttgttttt acaaatacca tttggctgtc gatgtaaata taggccacct
      601 cttcaatggg tagcgtaagc atgcctttag cggtttttac aagcaaacgg cttttgtatt
      661 cctttttcac aaaggaaatt tgcgccagca tttgttctat ttgcttgttt tgtgttgggg
      721 tggcaaatag tttcttgtac ttgtcgatgc tcttttgcag ggcagctttt tcaatgggtt
      781 taagcaggta atcaacactg ttcacttgaa atgcttttat cgcgtactcg tcgtacgagg
      841 tggtgaagat taccggatat tctacttttg tttctgaaaa aatctcgaag cttatcccgt
      901 cggagagttg tatgtccatg aaaattaaat cgatagttgg gttctccaat aagtattcga
      961 tggtttcttc aacgctacca atcactctaa gtacattcag tccgggctca atggttcgga
     1021 gcattttttc tagtccttgt gcggcataaa tttcgtcttc aactattaaa acgttcatat
     1081 cgatctagtt tataggtagg aattttcgtt tagcaaaggt aatttaacgg tgaagtgaga
     1141 ttccgattgt tcaataataa caggttgatc tgaaattaat tgatacctgg ttatgatgtt
     1201 ttgcaagcca atttttgttg aattctcaat gctcgatttc ttctgaaggt tgttttcaac
     1261 caccaattgc aaaccacggg ttttgatgct aatgatcagt ggttttctgt cggaaataat
     1321 gttgtgctta acggcgtttt caatcaacaa ttgtaacgag agcaggggca gatagtggtc
     1381 gtatttaggt tgttctatgt cgatgttgag tttaacacta tcgttaaagc gtatggcaat
     1441 gaggaacatg tatgcgttaa caaaatccat ctcttcacgc aactgaacca attctttgtc
     1501 cttgcccata agtatgtagc ggtaaacgtt cgaaaggtta cgggtgtatt cttcggcaag
     1561 ttcggggttg gccggaataa gttgaatgag cgtactgaat gtgttgaata agaaatgcgg
     1621 attaagttgg tttttaagtg cttcgaattt gaattgaata ttttcgcgtt gtagaatttc
     1681 agaagaaaga agtgttcgtt tcaactcggc ttctatttgg tcgcgttggt tcatcacttt
     1741 ttctttctgt agctgcaatt caatattgag ttgttcgagt aaattgtgtt gcgtaagtat
     1801 ttctttattc tgcttctcga tcttgagttg tttttcattc aaaatgcggt tggcattctg
     1861 gtttttttta aacaacctgt agtaaactat tgtgaaaacc gccaccaata gtaacattaa
     1921 aagtgcaata ctaataaccg tgcgttgttg ctttatttgc aatttcgatt tctcaaggtt
     1981 taatgtgttg atttcgttgg tgttgtttag taattttatt tcgttatcct tggcaattgt
     2041 ttcgtatttg gtttgtagct ctagcagttg tttttgcttt tgatggctgt aaatagtgtc
     2101 tgacgaggca tggtattttt ggtaaaaggt tagggcctgt ttatggttgt ttagtttttc
     2161 ataggttgta gaaaggttga ggtagctttc cattttcagt cgttcgtcgc ccgatttttc
     2221 gccacattcg agcgctttct taaacgcctc catggccagt ggcagattgt ttttgtcgga
     2281 atacaaatat cccttacttg tgtaacttcg gcctgcgcct tttatgtcgc tcatttcaag
     2341 cctaagcgaa attgcttttt caatgtattt tagtgcttcg ccgtagtttc ccatggagcg
     2401 gtataccgag cccaggtttc cgaatgaaat agcaatgtcg cgttttgagt cgaggttggt
     2461 ataaatttca agggccaatt gcatataatg aagcatggag tccagctgtt tttgtctcga
     2521 aaatattacc ccaatgttgt tgtaaatatt tgcaatgcca atgttgtcgc caatttcttt
     2581 tttaatcgat attgatttat tgtagttttc aagcgacttg tcgtagtctt tttggtaagt
     2641 ataaattatg cctatgttgg ttaaaagtcg cgacaattgg cttttttcgc ccagttcaat
     2701 taaaatagga taggctttat tgtaaaattc gaggcatttt gtatagtcgc ctttctgatc
     2761 gtatatgaga cctaaattat tgtaagcccg tgcaatacct tttttgttgt tgctaatact
     2821 atcgattttg tacgactttg taacattttc aagtgcttta tccagatcat caatattata
     2881 gtaggtcatg ccc
//
LOCUS       DAOPUW010000205         2891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_35681_length_2891_cov_4.356135, whole genome shotgun sequence.
ACCESSION   DAOPUW010000205 DAOPUW010000000
VERSION     DAOPUW010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35681_length_2891_cov_4.356135"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PKJ43_04425"
     CDS             <1..858
                     /locus_tag="PKJ43_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ71842.1"
                     /translation="YSRMAQHVHLLTTSGISLPTDLWLPVTKTFAPPISDQVALGTAI
                     NLPKDLTLTVEGFYKTMENLIEYKEGATFVGSSTDWESKVEKGRGWSYGLEMMLEKTV
                     GKTTGWLAYTLSWSQRQFENLNFGRMFPAKYDSRHDISIALTHTFNKRIDMGATWVFN
                     TGNAVTLGTREYIGADVPSGNDNLHGRPYITEYQGRNNYRMPPYHRMDWGINFHKQKK
                     HGVRTWNISIYNVYSRQNAFVLTWDTKREMHTIVQDGREIRVMGDKTILKKFSLYPII
                     PSFSYSYKF"
     gene            869..1945
                     /locus_tag="PKJ43_04430"
     CDS             869..1945
                     /locus_tag="PKJ43_04430"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HNZ71843.1"
                     /translation="MRISDKIYCVLVLLLFSALFTACSTEVEFKGEELEPKLVVNSIC
                     LVDSLLEVEVSATKLIPGFESKFRFIPNATVKVFVDDEETEVLQCVNVGDSVRAKLED
                     LFLARYSGKTVVKSGKKYRVEVSHPDFKHVAVGEMTGAMPVLIESAFVEVVKNDDILD
                     NSSSKVNASITFTDTPDEENYYRLYVYLSVGEGFETTKPNGEEGIRVLVMDYFVNQQL
                     TSSDPVFSNNNDPNNILGETSSFGYTIFDDKLLKGKPYELSFELYEHWAWDMAHVDTT
                     MGGYYIVHFELQSITKDVYYYLKSIDDSNESGMGLFTEPVQVYSNIANGLGIFGAASS
                     STYNIQYGRYPIDGVDYSYGMISY"
     gene            complement(2007..2633)
                     /locus_tag="PKJ43_04435"
     CDS             complement(2007..2633)
                     /locus_tag="PKJ43_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71844.1"
                     /translation="MTEEMPVVIECPSCGERYLIWPAMSELPQGTTYYSDGYFTSDNK
                     WRTPEIIGCVTCELGFTPQNGKIIATPNREEFLQHWSHLKPAQPPTTGALVLELRARK
                     NMDTTMEKVLRKELWYSTSHTTAGKNLLENNAKFRNFWNESMIRLETLFDRNNPAELL
                     LKAEANRQLGHYEQCLELLLGEQSNMAEAIRNAAQRENCTLLVIAEKK"
BASE COUNT          768 a          557 c          667 g          899 t
ORIGIN      
        1 tattctcgaa tggcacagca tgtgcatttg ctcacaactt cgggcataag tttgcccacc
       61 gatctttggc tgccggttac caaaacgttt gcgccaccta tttcagacca agtggcgttg
      121 ggcacagcca ttaatctccc taaagactta acgcttaccg ttgagggctt ttacaaaacc
      181 atggaaaacc ttatcgaata caaagagggg gcaacatttg tagggagttc aaccgactgg
      241 gaaagcaagg ttgaaaaagg ccgtggctgg tcgtacggac tcgaaatgat gcttgaaaaa
      301 acggttggta aaacaactgg ttggctggct tataccctct cgtggagtca gcgccagttc
      361 gagaatctta attttggacg catgtttcct gctaaatacg acagcaggca cgacattagc
      421 attgccctta cgcatacttt caacaagcgg attgatatgg gggcaacctg ggttttcaac
      481 accggaaacg ccgtaactct tggaacccga gaatatattg gagctgacgt gcccagtgga
      541 aacgataatc ttcatggaag gccatatatt acagaatacc aggggcgtaa caattaccgc
      601 atgccaccat atcatcgtat ggattggggt ataaatttcc acaaacaaaa aaaacatggt
      661 gtgcggacat ggaacatctc tatatacaat gtgtatagtc ggcaaaatgc gtttgtgctt
      721 acctgggaca cgaaaaggga aatgcatact attgtacagg atggacggga aattagggtg
      781 atgggcgata aaaccatctt gaagaaattt agcctctatc ctattattcc atcgttttct
      841 tattcgtata aattttaatt tactagtaat gagaatttca gataaaatat attgtgtgct
      901 ggttttgctg cttttttcgg cattgttcac ggcttgttct acagaagtag aattcaaagg
      961 agaagaacta gaaccaaaac tggttgttaa cagcatttgt ttggtagata gtttgctcga
     1021 ggtagaggtt tcggcaacaa agctcattcc cggatttgag agtaaatttc gctttattcc
     1081 taatgcaact gtaaaagtct ttgtagatga tgaagaaacc gaagtattgc aatgtgttaa
     1141 tgtcggtgat tcggtgagag caaagttgga agacttgttt ttagcccgtt attcaggaaa
     1201 aacagtggtc aaaagcggga aaaagtaccg cgttgaggtg tcgcatcccg attttaaaca
     1261 cgtagctgtg ggtgaaatga caggtgcaat gccggttctc atcgagtctg cttttgttga
     1321 ggtggttaaa aatgatgata ttcttgacaa ttcatcgtca aaagtgaatg ccagcatcac
     1381 gtttaccgat acgcccgatg aggaaaatta ttatcgcctt tatgtttatc ttagtgtggg
     1441 ggaggggttt gagacgacca agccgaatgg tgaagaaggt attcgtgttc ttgtgatgga
     1501 ttactttgta aatcaacaac tcacgtcttc agaccctgtg ttttcaaata acaacgaccc
     1561 caacaatatt ctgggcgaaa catcatcgtt tggctatact atcttcgacg ataaattgct
     1621 aaaaggtaaa ccctacgaat tgtcgttcga gctttacgaa cattgggctt gggacatggc
     1681 tcatgttgac accactatgg gcgggtatta tattgttcat ttcgaattgc agtcgataac
     1741 caaggatgtg tattattacc taaagtcaat tgacgattca aacgaatcgg gcatggggct
     1801 tttcaccgaa ccagtacagg tatatagcaa cattgctaat ggcttgggta tttttggagc
     1861 tgcatcttcg tctacttaca acattcagta tggccgctat cctattgacg gtgttgacta
     1921 ctcctatgga atgataagtt attaacctga gttcgatttt atttttaaag ttcatattgg
     1981 cgtaaagtat tgttttccag tggttcttat ttcttttctg caataaccaa aagcgtgcag
     2041 ttttcgcgtt gggcggcgtt tcttatggct tcggccatgt tgctttgctc gcccaataaa
     2101 agctccaggc actgttcata gtgccccaac tggcggttag cttctgcttt tagcaacaat
     2161 tctgccgggt tattcctgtc gaaaagtgtt tcgagccgta tcatgctttc gttccagaag
     2221 tttctgaatt tggcgttatt ttccaacagg tttttcccgg ctgtggtatg cgaagtgctg
     2281 taccataatt cttttctgag aactttctcc atcgtagtat ccatgttttt ccgggcacgt
     2341 aactcaagta ccaaggctcc ggtggttggg ggctgtgccg gttttaggtg cgaccagtgt
     2401 tggagaaatt cttcacgatt tggggtggca attattttgc cgttttgagg ggtaaaaccc
     2461 agttcgcagg ttacacagcc aattatttcg ggtgtgcgcc acttgttgtc cgaagtgaaa
     2521 tagccgtcac tgtaataggt ggttccttgc ggtaactcgc tcatcgctgg ccaaataaga
     2581 tacctttccc cacagcttgg gcattctatc acaacaggca tttcttcagt catatagtta
     2641 aggtgttatg gtttttgttc ggtacaaaaa aatattgtga agatcgttgt gcgtcttcac
     2701 aatattatat tatcgcaaga tgaaaactat gaatagagga acctttttat gttctttttc
     2761 ggagtttttt caaactcttc atttacaatt tcaattttcg atagctgttc gtattgagct
     2821 acgtgatgat tgaaattttt acggttatcg gccattattg tgtttaatgc attttcgtcg
     2881 atgttgttgg t
//
LOCUS       DAOPUW010000206         2891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_35682_length_2891_cov_3.651269, whole genome shotgun sequence.
ACCESSION   DAOPUW010000206 DAOPUW010000000
VERSION     DAOPUW010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35682_length_2891_cov_3.651269"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(293..670)
                     /locus_tag="PKJ43_04440"
     CDS             complement(293..670)
                     /locus_tag="PKJ43_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNZ71845.1"
                     /translation="MTAGVRIDKWLFAVRIFKTRTIATDECNKGRITIEGMNVKPSRE
                     VRIGDVIEIRKPPITYKFKVLQLAEKRMGAKLVPEFAEDLTTKEQFEIIEMQRMVQWS
                     GREKGTGRPTKKDRRDMEDFLDW"
     gene            complement(673..1239)
                     /gene="pth"
                     /locus_tag="PKJ43_04445"
     CDS             complement(673..1239)
                     /gene="pth"
                     /locus_tag="PKJ43_04445"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNZ71846.1"
                     /translation="MKYLIVGLGNIGEEYAKTRHNVGFQILDALARASNLVFDVNKRY
                     GAIAEYKFKGHIFILLKPSTYMNLSGNAVRYWLQKENIAPENLMVLVDDLALPFGAIR
                     IRTKGSDAGHNGLKHINETLGSNEYARVRFGIGNQFNKGQQVDYVLGEWSEEESNLLP
                     ERIDKVVQIIKSFGTIGIERTMNQFNNK"
     gene            complement(1274..1867)
                     /locus_tag="PKJ43_04450"
     CDS             complement(1274..1867)
                     /locus_tag="PKJ43_04450"
                     /inference="COORDINATES: protein motif:HMM:NF004132.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HNZ71847.1"
                     /translation="MKSFELKGNLREALGKKDSAKLRAEKKVPCVLYGVDEPVHFYTE
                     SADLAKLIYTPNVYIVDLTINGKKSQAIMKDIQFDPVSDEVTHIDFLKISDSKPIKIE
                     VPVNVKGFAKGIRAGGKLQIEVRRLKLLALPKYLPDTIEIDVTELDLGQSLRVSDIHL
                     DNVTILNGKSIPVVRIMVTRAARAAAQAAETSGKGKK"
     gene            complement(2058..>2891)
                     /locus_tag="PKJ43_04455"
     CDS             complement(2058..>2891)
                     /locus_tag="PKJ43_04455"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNZ71848.1"
                     /translation="PRFSDGEFEPCYEETIRGSYVFIVQSTFPSADNLWELLLMIDAA
                     KRASAYKVIAVIPYFGYARQDRKDKPRVSIGAKVVADVLSKAGVDRVITVDLHADQIQ
                     GFFDVPVDHLYSSSLFIPYIKQMNLPNVVIASPDVGGTKRANTYAKLLETDLVICHKT
                     RSKANYIDNMTVIGDVTGKDVIIVDDIIDTAGTISKAADLFKERGATSVRAFATHPIL
                     SGPAIERIENSALSEVYFTDTIPLKKESEKIKILSIAPLCADVIEKVYYYKSISSIYT
                     Q"
BASE COUNT          843 a          605 c          517 g          926 t
ORIGIN      
        1 aatccccctc ttcattcccc taaaccttta aaaaaatttg cttaagcaaa aaactacact
       61 agaaagataa caaaataaat taagaaagaa aagaaccctg atcaccaaac aataaagggg
      121 aaagctattt tttcttattg cttattttat agaaaacaga agccattata accttctcgg
      181 aattgagcaa aaagtaaaat aggaaataat aataggggga taatgagctt taaggaattc
      241 attttaaaat atttaggggg tgaatatttt tacttatacc aagtcgatgt tattaccagt
      301 cgagaaaatc ttccatgtcg cggcgatctt tttttgtagg cctgccagtt cccttttctc
      361 gtccactcca ctgaaccatt cgttgcattt caataatttc aaattgttct tttgtggtaa
      421 ggtcttcggc aaattcagga acaagttttg cccccatgcg tttctctgcc aattgaagca
      481 ctttaaattt ataggttatt ggaggttttc ttatttcgat aacatcgcca atccgaactt
      541 ctctcgaagg cttaacgttc attccttcaa tggttatgcg gcctttgtta cactcgtcgg
      601 tagcgattgt tctggtttta aatatcctga cggcaaatag ccatttgtca attcgaacac
      661 cagcagtcat agctatttat tattaaattg gttcatggta cgttcaatgc ctatagtgcc
      721 aaacgattta ataatctgaa ctactttatc gattctctcg ggcaacagat tggattcctc
      781 ttcagaccat tcgcctaata cataatctac ctgttgtcct ttattgaatt gatttccaat
      841 accgaaccta acccgggcat attcatttga gccaagagtc tcgttgatgt gttttaaccc
      901 gttgtgtccg gcatcgctgc ctttggttct tatccgaatg gcgccaaaag gcaaagcaag
      961 gtcatcgacc aaaaccatta agttttcagg ggctatgttt tctttctgca accaataacg
     1021 cacggcattg ccgctaaggt tcatataggt cgaaggtttg agtaagatga aaatatgccc
     1081 tttaaactta tattctgcga tagctccata ccgcttgttt acatcaaaaa caagattgga
     1141 cgccctagcc agagcgtcca atatttgaaa gccaacattg tggcgagttt ttgcatattc
     1201 ttctccgatg ttaccaaggc caacaatcag atacttcata attgcatttt ttacgggtat
     1261 agtaccctat aggttatttc tttccttttc cacttgtttc agcagcctga gcagcagcac
     1321 gtgcagcacg ggttaccata atgcgaacaa caggaattga tttgccattg agaattgtta
     1381 cattatcaag gtgaatatcg ctaacacgta aactttggcc aagatcgagt tcagttacat
     1441 caatttcaat cgtatcgggc aaatattttg gtaaagccaa taatttcaag cggcgaactt
     1501 caatttgcaa tttaccccct gcacgtatac cttttgcaaa acctttaacg ttaacgggaa
     1561 cttcaatttt tatcggtttg ctatcgctaa ttttcaagaa gtcgatgtga gttacttcgt
     1621 cgctaacagg atcaaactga atgtctttca tgatcgcttg cgattttttc ccattgatag
     1681 taagatctac tatatacaca ttgggagtgt aaatcaattt ggctaaatcg gcgctttcag
     1741 tataaaaatg aacaggttca tcaacaccat ataacacaca tggtactttc ttttctgctc
     1801 tcagctttgc tgaatctttt tttcctaacg cttcacgaag gtttcctttt aattcaaaac
     1861 ttttcatttt ctttttgttt tgtggtactc agcccttccc gatattcccg gaaatcccgg
     1921 tacgggacaa accgcataca cgattaataa ttgtctaatt tttaaagcgg tgcaaatata
     1981 aatcttttta tcaaaaaaac agtctaccaa taaaataaag gtagactgac atatctttaa
     2041 tgcgataaca caaaatgtta ttgcgtataa atggaactaa ttgatttgta ataatatacc
     2101 ttttcaatca catcggcaca caaaggggca atagataata ttttaatttt ttccgattct
     2161 ttttttaatg gaattgtatc ggtaaagtat acttcgctaa gagcagaatt ctcaattcgc
     2221 tcgatggctg gccccgaaag aattgggtgt gtggcaaaag ccctgacact tgttgctccg
     2281 cgttctttaa acaagtctgc ggctttactt attgttccgg ctgtgtcaat aatatcatca
     2341 acaatgataa catctttgcc cgttacatca ccaataactg tcatattatc aatgtaattt
     2401 gccttgctac gtgttttatg gcaaattacg aggtctgttt cgagcaattt tgcatacgtg
     2461 ttggctcgtt ttgttccccc aacatcgggc gaagcgataa ccacgttagg caaattcatt
     2521 tgctttatat atggaataaa caacgacgat gaatagaggt gatcaactgg cacatcgaaa
     2581 aatccctgga tttgatcggc atgcaaatca acagtgataa ccctgtcgac accggctttt
     2641 gaaagcacat cggcaactac ttttgcccct atagaaacac ggggtttatc ttttcggtct
     2701 tgacgggcat acccaaaata tgggatcact gcaattactt tgtacgctga tgcccgtttg
     2761 gcggcatcaa tcatcagcaa taattcccat aagttatcgg ctgatggaaa agttgactga
     2821 acaatgaaaa catacgaacc cctgatggtt tcttcgtagc agggttcgaa ctctccgtcg
     2881 ctgaaccttg g
//
LOCUS       DAOPUW010000207         2891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_35683_length_2891_cov_3.635049, whole genome shotgun sequence.
ACCESSION   DAOPUW010000207 DAOPUW010000000
VERSION     DAOPUW010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35683_length_2891_cov_3.635049"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PKJ43_04460"
     CDS             complement(<1..451)
                     /locus_tag="PKJ43_04460"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNZ71849.1"
                     /translation="MSVNSFNPIIKLLSVDSTNNYATARLETENWTEGTLVLSENQTA
                     GRGQVNNSWQSETGKNILASMVFYPTFLPIQFQFLLSKTIALAIHELVSLYADNVSIK
                     WPNDIYIGQKKVAGILLETAILGSKINWAVAGIGLNVNQKVFSSGLPN"
     gene            511..885
                     /gene="rsfS"
                     /locus_tag="PKJ43_04465"
     CDS             511..885
                     /gene="rsfS"
                     /locus_tag="PKJ43_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNZ71850.1"
                     /translation="MDQTNNNTSNTLIDAIVEGVRRKKGESIVDLDMIMLENSQCDHF
                     IICHGNSNTQVEAIARSVEETVEEYTGQNAWHADGYRNAQWILLDYSDVMVHVFQKEF
                     RNHYDLESLWADAQINIYKSEN"
     gene            893..>2891
                     /gene="ftsH"
                     /locus_tag="PKJ43_04470"
     CDS             893..>2891
                     /gene="ftsH"
                     /locus_tag="PKJ43_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNZ71851.1"
                     /translation="MAEDNSKKNNQQSKDQEQPQKQDPNLKMKPNNGKGKFNMTFFYL
                     ALILILLGINLFMSGNNAVETTWHEVKTTMLKNNDIQKIVVLNKTNANIYLKEESFEK
                     YKDKMGISGFNKPSKHGPQFYFVIGSVDKFEENIEKAYQEYSISNSDYLPIIYSDTFD
                     WGSVISFLLPIAIIIFFYWFIFRRMSKGGGGPGGNIFSVGKSQAKLFDKDQKVSTTFK
                     DVAGLSEAKQEIEEIVSFLKNPSKYTKLGGKIPKGALLVGPPGTGKTLLAKAVAGEAN
                     VPFFSMSGSDFVEMFVGVGASRVRDLFKQAKEKAPCIVFIDEIDAIGRARGKNPNMGS
                     NDERENTLNQLLTEMDGFGTNSGVIILAATNRADILDRALMRAGRFDRQIHVELPDLN
                     ERKEIFNVHLRPIKISEEINSDFLSKQTPGFSGADIANVCNEAALIAARNNHEKVEKQ
                     DFMSAVDRIVGGLEKKNKIISIEEKRTIAYHEAGHATVSWLLEHAHPLVKVTIVPRGK
                     ALGAAWYLPEERSITTKEQMLDEMCATLGGRAAEELAFSSISSGAQNDLERVTKQAYA
                     MVCYFGMSEKVGNLSYYDSSGQSDYGFTKPYSEKTAELIDEEIKNLIGEAYVRAKEVL
                     SANSTGHKKLADRLLEKEVIFSDDLEEIFGPRNSSHQHSIAD"
BASE COUNT          983 a          566 c          593 g          749 t
ORIGIN      
        1 gattaggcaa acctgaacta aaaactttct gattaacatt cagaccaatc cctgccacag
       61 cccaattaat tttacttccc agaatagcgg tttcgagcag aatacctgcc acctttttct
      121 gtcctatata aatatcattt ggccatttaa tagacacatt atctgcatat aatgaaacta
      181 attcgtgaat agccaaggca atagtcttcg acaataaaaa ttgaaattga attggcagaa
      241 acgtagggta gaaaaccatg ctggccaaaa tattctttcc tgtttcactc tgccatgagt
      301 tattcacctg accacgacca gctgtttggt tttcgcttaa aaccaaagtc ccttcagtcc
      361 aattttctgt ttcaagcctt gcagtggcat agttattggt cgaatcaaca gacagtaatt
      421 ttatgattgg attaaaagaa tttactgaca taatttcagg tgtattaatt aggtaaaaag
      481 attaattttg caaagtgcta aaatacaaat atggatcaaa caaataacaa cacatcaaat
      541 acattaattg acgcaattgt tgaaggagta agacgaaaaa agggagaaag cattgttgac
      601 cttgacatga taatgctaga aaattcacaa tgtgaccatt ttataatctg ccacggaaat
      661 tcaaacaccc aagttgaagc aatagcccgt tcagttgaag aaacagtaga agaatataca
      721 gggcaaaatg catggcatgc ggatggttac agaaacgcac aatggatttt gcttgactat
      781 tctgatgtaa tggttcatgt atttcaaaag gaattccgca accattatga tcttgaatca
      841 ctctgggcag atgcacaaat aaacatttac aaatcagaaa attagataag aaatggctga
      901 agataattcg aaaaaaaata accagcagag caaggatcag gaacaacccc aaaaacagga
      961 tccaaacctt aaaatgaagc caaacaatgg taaaggtaag ttcaatatga ccttctttta
     1021 tctggctcta attctcatac tactgggaat aaatttattc atgagtggaa acaatgccgt
     1081 tgaaaccact tggcatgagg ttaaaaccac catgttgaag aataacgaca ttcagaaaat
     1141 tgttgttcta aataaaacca atgcaaatat ttacctcaaa gaagaaagtt ttgagaaata
     1201 taaggataag atgggtatta gcgggtttaa caagcctagc aagcatggcc cacagtttta
     1261 ctttgtaatc ggctctgttg ataagtttga agaaaatata gaaaaagcct atcaggaata
     1321 tagcatatca aacagtgatt accttccaat aatatacagc gatactttcg attggggaag
     1381 tgtcatcagc tttttactgc caatagcgat cattattttt ttctattggt ttattttcag
     1441 aaggatgtca aaaggaggcg gaggcccggg cggaaatatt ttctcggtag gaaaatcaca
     1501 agcgaagctc ttcgacaaag accaaaaagt atctaccact tttaaagatg ttgctggtct
     1561 ttcagaagcg aaacaagaaa ttgaagaaat tgtatccttc cttaaaaacc cttcaaaata
     1621 cacaaaactg ggaggtaaaa tacccaaagg tgcattgctt gttggccctc cgggaacagg
     1681 taaaactttg ttagccaaag cagtggctgg cgaagcaaac gtgccgtttt tctctatgtc
     1741 gggttccgat tttgtggaaa tgtttgttgg ggttggtgca tcacgcgttc gtgacctctt
     1801 taaacaagcc aaagaaaaag ccccgtgcat tgtttttatc gatgaaattg atgccattgg
     1861 ccgtgcccgt ggaaagaacc cgaatatggg gtcaaacgat gaacgagaaa acactcttaa
     1921 ccaattactt accgaaatgg acgggttcgg caccaattca ggggtaatca ttttggcagc
     1981 cactaaccgt gccgatattc ttgatcgggc attgatgcgc gctggccgtt tcgatcgcca
     2041 gatacatgtt gaactccccg acttgaacga aagaaaagaa atattcaatg ttcacctgcg
     2101 tccaataaaa atatcggaag aaataaactc cgatttcctc tccaagcaaa caccaggatt
     2161 ctccggagcc gacatagcta acgtatgtaa cgaagccgcc ttgattgctg cacgaaataa
     2221 ccatgaaaaa gttgaaaaac aagattttat gtcagcagtt gaccgcatcg taggtggctt
     2281 ggagaagaag aacaaaataa tttccatcga agagaaaagg acaattgcct atcacgaagc
     2341 tgggcatgct acggtgagct ggctacttga acacgcccat cctttggtaa aagtaaccat
     2401 tgtacctcgc gggaaagcac ttggagcagc atggtacctg cctgaagaac ggtctattac
     2461 aacaaaagag caaatgctcg acgaaatgtg tgctaccttg ggtggccgcg ctgccgaaga
     2521 acttgcgttc agcagtattt cttcgggagc ccaaaacgac cttgagcgag ttactaaaca
     2581 agcctatgct atggtctgct attttggcat gagtgaaaaa gtgggcaacc tgagttacta
     2641 cgattcctca gggcaatcag actatggttt caccaaacca tacagcgaga aaacggcaga
     2701 acttattgac gaagaaatta aaaacctaat tggtgaggct tatgtgcgtg caaaagaagt
     2761 actatcggcc aattcaaccg gacacaaaaa actagcagat aggcttctgg agaaagaagt
     2821 tatttttagc gatgatttgg aagaaatctt cggcccaaga aatagttcgc accagcattc
     2881 cattgccgat g
//
LOCUS       DAOPUW010000208         2886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_35788_length_2886_cov_2.966090, whole genome shotgun sequence.
ACCESSION   DAOPUW010000208 DAOPUW010000000
VERSION     DAOPUW010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35788_length_2886_cov_2.966090"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PKJ43_04475"
     CDS             <1..336
                     /locus_tag="PKJ43_04475"
                     /inference="COORDINATES: protein motif:HMM:NF020334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HNZ71852.1"
                     /translation="KENKFSNRSQAIRQLINRNIIEHKWICNNFVAGSVMLIYDPHKR
                     EIMNQINTIQEDFIDEIISSQRFILSKNKCLEIIAVKGIANRLTSLSDALISVKGVLH
                     GKLSMSRAD"
     gene            412..1695
                     /locus_tag="PKJ43_04480"
     CDS             412..1695
                     /locus_tag="PKJ43_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNZ71853.1"
                     /translation="MKEIIIISVLFLLNGIFSMYETALLSSRKTKLEERARSGHSGAK
                     TALHLLLEPEKILSAIQVGITLVGIVSGAYGGIAFTEDVAPVFRNITFLAPYADSLAM
                     ILVVGLITYFSIIIGELVPKSIALNNPETIASFLSPAMRMAGIFAYPVVLLLSISTKM
                     VIKLFGIKSERDSIVSEEELRILLRQGSENGVIEKEESTIINEVIRFGEKRAGTIMTH
                     RLDVKWIDINSPIDEIIETIQNIPFSRLLVCNKDIEDIKGVVAIKDIFSSYINSKNID
                     FENIMHDPVYIPEQLPAVKVLEQFRETRNHFGVVVNEYGSIEGIITLHDVTENIMGDL
                     PAIEDQDEPEVFRREDGSYLIDGSMLIEDVEDLLDLPSLYDDNEDEANVNTIGGLAMY
                     KLNRIPKAGDKFTIKDRVFEIVDMDGNRVDKLNVI"
     gene            2053..>2886
                     /locus_tag="PKJ43_04485"
     CDS             2053..>2886
                     /locus_tag="PKJ43_04485"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNZ71854.1"
                     /translation="MKQSSTTGMLNRWMKQGIALVFVSLFTSLALYSQERTITGTVRD
                     ASGETLVGVNIVVKGTTQGTSTDIDGKFSLGGIKSDATLTANYIGYKSQDINVGSQST
                     FEITMEKDVMDLDEVVVVGYGVQKKKLVTGATVQIKNEEFTKNNSTRIENALQGLTPG
                     MLIVKQSGQPGSDYNITIRGLSSINGNSPLVLIDGVPGDMNMLNPSDIESIDVLKDAA
                     SAAIYGSRAANGVILVTTKKGRSGEATVTYDAYIGISNLSKKIDLLNAREYAEIMNEA
                     SL"
BASE COUNT          921 a          589 c          592 g          784 t
ORIGIN      
        1 aaagagaaca aattttcgaa ccgttctcag gcaatacgac aattaataaa ccggaacatt
       61 attgaacaca agtggatatg taataatttt gttgcaggct cggtaatgct catttacgac
      121 ccgcataagc gcgagataat gaaccaaata aacacgatac aagaagattt tattgatgaa
      181 attatttcat cgcaacgatt tatactcagc aaaaacaaat gccttgaaat aattgcagta
      241 aagggtattg ccaaccgttt aacatcatta tctgatgcac ttatttcggt aaaaggagta
      301 cttcacggca aactttcgat gagtcgcgcc gattaacatt gtattctttt tattgtattt
      361 cattatcctt agcgcacaag gataaaaata aggattacct ttgtattcgt tatgaaagag
      421 attataatca tttcagtact ttttctgctc aatggcattt tctcgatgta tgagacagcc
      481 ttactttcgt cacgcaaaac caagctcgaa gaaagagctc gttcaggcca ttcgggagca
      541 aaaacggcat tgcatttact gcttgaaccc gaaaaaatac tctctgccat tcaggtgggc
      601 attaccttgg ttggaattgt atctggtgct tatgggggaa ttgcatttac tgaagatgtt
      661 gccccggtct ttcgtaacat taccttttta gctccatatg ccgattcact ggccatgatt
      721 cttgtagtgg ggctaattac ctatttttca attattatcg gagagttggt acccaaatct
      781 attgcactga acaatcctga aactatcgca tctttcttgt cgccggcaat gcgcatggcg
      841 ggtatttttg cctaccctgt agttttgctc ctcagtattt ctacaaaaat ggtaatcaag
      901 ctattcggca ttaaatcgga acgtgattca attgtttcgg aagaagaatt acggattttg
      961 ctcagacaag gttcagaaaa cggggttatc gaaaaagaag agtcaaccat aattaacgaa
     1021 gttatccggt ttggcgagaa acgtgctgga acaatcatga cccatcggct tgatgtgaaa
     1081 tggatcgaca taaactcacc gattgatgaa attattgaaa ccatacaaaa cattccgttc
     1141 tcgaggttgc tggtttgcaa taaagacatc gaagatataa aaggagtggt ggccattaaa
     1201 gacatctttt cgagttatat aaattcgaaa aatattgatt ttgaaaatat tatgcacgat
     1261 cccgtatata ttccagagca acttcctgca gtaaaagttc tcgaacaatt cagggaaacc
     1321 agaaatcatt ttggggttgt tgtgaatgaa tatggctcga tcgaagggat tattaccctt
     1381 cacgatgtta ccgaaaacat tatgggcgac ttgcctgcga tagaagacca agacgagccc
     1441 gaagttttca ggcgcgaaga tggatcatat ttaattgatg gatcaatgct cattgaagat
     1501 gttgaagacc tcctcgattt accttcgctt tacgatgaca acgaagacga ggcaaacgta
     1561 aataccattg gtgggttagc catgtacaaa ctcaaccgga tacccaaagc cggtgacaaa
     1621 tttaccataa aagaccgcgt attcgaaatt gttgacatgg atggaaaccg tgtcgataag
     1681 ctgaacgtga tttaaccctt ttgttggaaa accttcacac acagcccttt atcattctaa
     1741 cctcaataca taaaacgcta tagttctgtt ggttctcagc aagaacactc ctattgtttg
     1801 ttgacaaata caacacgtat tgaatacaaa tcaacaacat ccactttagc aattttaagt
     1861 aattatgctt taataacgag agcacgataa tgccttcatt gacaaactgt agcttcacct
     1921 ccaatttcct gtaataatta ttgagtaatg aagcttcgtc cccaacgcag gaactaatcg
     1981 gcctcaaaaa cgaaaaccga tagaaaatct aataatctat taataaacag caacaaaatc
     2041 taaatgaatc taatgaaaca atcatcaaca accggtatgt tgaatcgctg gatgaaacag
     2101 ggaatagccc ttgtttttgt cagcctattc acaagcctcg cgctgtattc gcaagaacgg
     2161 accataacag gtaccgtacg tgacgcatcg ggtgaaacac tcgtaggggt taatattgta
     2221 gtgaaaggca ctacccaggg aacctcaacc gacatcgacg gaaagttcag cctggggggc
     2281 ataaaaagcg acgctactct taccgccaac tacatcgggt acaaaagcca ggacataaat
     2341 gttggcagcc agtcgacttt tgaaatcact atggaaaaag acgtaatgga tctcgatgaa
     2401 gtagtggttg taggttacgg tgtacagaaa aagaagctgg tcaccggagc aactgtccag
     2461 attaaaaatg aagaatttac aaagaacaac tcaacccgaa tcgaaaacgc gctgcaaggc
     2521 ctgactcctg gtatgcttat cgtgaaacaa tcggggcaac ctggctcaga ttacaatatc
     2581 actatcaggg ggctttcttc aatcaatggc aacagcccat tggtacttat cgacggggta
     2641 ccgggcgata tgaacatgct aaacccttct gacattgaaa gcattgatgt tttgaaagat
     2701 gctgcttcgg ctgcaatcta tggatcgcgc gctgccaatg gggtaatcct tgtgaccacc
     2761 aagaaagggc gctcgggcga agcgactgta acatacgatg cttacatcgg aatttcgaac
     2821 ctttcgaaga aaattgattt gctcaatgcc cgtgagtatg ctgaaattat gaacgaagcc
     2881 tcgtta
//
LOCUS       DAOPUW010000209         2883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_35835_length_2883_cov_3.280057, whole genome shotgun sequence.
ACCESSION   DAOPUW010000209 DAOPUW010000000
VERSION     DAOPUW010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35835_length_2883_cov_3.280057"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PKJ43_04490"
     CDS             complement(<1..549)
                     /locus_tag="PKJ43_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ71855.1"
                     /translation="MKLKTIAIDDEPLAVALLTDYINKTPFLELSGTFDNPLDAIEFL
                     HQETVDLILVDIQMPDLNGVEFVRTLESAPKVIFTTAYAQYALEGFKLNATDYLLKPF
                     GYEDFLKAVQKALKQIEIEHAAATSVEANNQFLFLKSEYKIRRINFNDILYIEGLKDY
                     IKVFIKGEAKPILSLSSVKAIES"
     gene            complement(546..1637)
                     /locus_tag="PKJ43_04495"
     CDS             complement(546..1637)
                     /locus_tag="PKJ43_04495"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNZ71856.1"
                     /translation="MNKLILTDNRLKIALHIVLWVIIFIIPIYISRAVGFAEIRPLWH
                     FYTIAIAYGIIFYVSYLYLVPEFYFKKAKWQFFLIVVVLIAVLFILIIAVNDFLLADP
                     ERDRHLEEVMKRINENRNYKRPPMGLFRLVNYAFTSLMIVGFALGLGFMDRHREDEQK
                     RKELEKEKLHSELAFLKNQISPHFFFNTLNNIYSLTTIDTPAAQESILKLSKLMRYLL
                     YESEQGETMLSHEIDFMVNYIALMKLRLSPKVELKVSFPTEYPDCKIPPLLFISFIEN
                     AFKHGVSHRETSFIHIEMKADSQQILFKTENSVSGTASTDKQNSGIGLENVRKRLALL
                     FPTSHTLKIEQTDKAFNVEVKVELKKTEA"
     gene            complement(1640..2482)
                     /locus_tag="PKJ43_04500"
     CDS             complement(1640..2482)
                     /locus_tag="PKJ43_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HNZ71857.1"
                     /translation="MTKETAIKLFNEKQIRTHWDDEQEKWYFSIVDVVGVLSESPNPN
                     NYWKVLKHRLVKEGSELVTNCNQLKMQSTDGKFYKTDVADTEQLFRLIQSISSPKAEP
                     FKMWLAKVGRERIDEIEDPELGIDRMMETYLRKGYSKEWINQRLKSIEVRKELTDEWE
                     NRGVQKGQEYAILTDEITKAWSGLTTKQYKKFKELKKENLRDHMTNLELVLNMLAEAT
                     TTEISKEKKPKTFEQNRIIANQGGTIAGNTRKEIEEKTGKKVVTSKNAKQLSSGKINE
                     MENS"
     gene            complement(2599..>2883)
                     /locus_tag="PKJ43_04505"
     CDS             complement(2599..>2883)
                     /locus_tag="PKJ43_04505"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNZ71858.1"
                     /translation="GRLVESAVGAHLINYSVSERFQLYYWREGNNEVDFVLEHRGKTV
                     ALEVKSGSNTINKGMDLFAQKYNSAKVLMVGSGGIPLELFLRMNPLELFR"
BASE COUNT          785 a          628 c          546 g          924 t
ORIGIN      
        1 gctttcgatg gctttaaccg aactgagcga aagaatgggc ttggcctcgc ccttaatgaa
       61 tactttgata taatccttca gcccttcgat gtaaaggatg tcgttgaagt tgatgcgacg
      121 aattttgtac tccgacttca aaaacagaaa ctggttgttg gcctctacgc ttgtagcggc
      181 ggcgtgttca atctcaattt gcttcaaggc cttttgaaca gcctttaaaa aatcctcgta
      241 tccaaagggt ttcaacaaat agtcggttgc attcagttta aaaccttcga gtgcatattg
      301 cgcgtaggct gtggtaaaaa tcaccttagg agcgctctcg agtgtacgaa caaattcaac
      361 accatttaaa tcaggcattt gaatatcaac caaaatcaag tcaacggtct cttgatgcaa
      421 aaactcaatg gcatcaagcg gattgtcgaa cgtgccggac aactcgagaa atggagtctt
      481 gtttatgtag tcggtcaata gcgcaacagc taaaggctca tcatctattg caatggtttt
      541 caacttcatg cttcagtttt tttgagttca actttaactt caacattgaa tgctttatcg
      601 gtctgttcaa ttttcaacgt atgcgaagta gggaaaagca aagccaggcg tttccgcaca
      661 ttttcgagcc cgatacccga attttgtttg tcggttgatg ctgttcccga aactgaattc
      721 tcggttttaa ataaaatttg ctgactgtcg gccttcattt caatgtgaat aaaagaagtt
      781 tcgcgatggc ttactccgtg tttaaaagca ttctcgatga acgagataaa caatagcggc
      841 ggaattttgc aatcgggata ttctgtcgga aacgaaacct tcagttcaac ctttgggcta
      901 agccgcagtt tcatcagggc gatatagtta accatgaagt cgatttcgtg gctgagcata
      961 gtttcgccct gttccgattc gtataacaga tagcgcatca gtttcgacag tttcaaaatc
     1021 gattcctgag cagcaggcgt gtcgatggtg gttaacgaat aaatgttatt taaggtattg
     1081 aaaaagaagt gcggactgat ttggtttttc aaaaaagcca attcggaatg cagcttttct
     1141 ttttccaatt cttttcgctt ctgttcgtct tcgcggtgac gatccataaa ccccagccca
     1201 agggcaaaac cgacaatcat tagcgaagta aaagcatagt tgaccaaacg gaacaatccc
     1261 attggcggac gtttgtaatt tcggttctca ttgattctct tcatgacttc ttctaagtga
     1321 cggtcacgtt caggatcggc aagcaaaaag tcgtttacgg caataatcag tataaacagc
     1381 accgctatca gcactacaac aatcaagaaa aattgccatt ttgctttctt aaaatagaac
     1441 tccggaacca gatataaata actgacatag aatataattc cataggcaat agctattgta
     1501 tagaaatgcc ataagggtct gatttcagca aatccaacag cccgtgaaat atagattggg
     1561 ataataaaaa tgatgaccca aagcacaatg tgcagggcaa ttttcaatcg attatcggtg
     1621 agtatgagct tgttcattat taactatttt ccatttcgtt gatcttgcca gaactaagct
     1681 gtttggcatt tttgcttgtt accacttttt tccctgtttt ctcttcaatt tccttccggg
     1741 tgtttccggc aatggttccg ccctgattgg caattatcct attctgttca aacgttttgg
     1801 gtttcttttc cttcgaaatt tcagttgtag tggcttcggc aagcatgttc agcaccaact
     1861 caaggttggt catgtggtcg cgaaggttct ctttttttaa ctctttgaat tttttatatt
     1921 gcttcgttgt aagtccgctc catgcttttg taatttcatc ggtaagaatc gcatattcct
     1981 gtcctttttg tacacctcgg ttttcccatt cgtcggtcag ttctttgcgt acctcaatac
     2041 ttttcaaacg ctggttaatc cactctttgc tgtatccttt gcgcaagtag gtctccatca
     2101 tgcgatcgat tcctagttct gggtcttcaa tttcgtcaat ccgttcacgt ccaacttttg
     2161 ccaaccacat tttaaaaggt tcagctttgg gcgacgatat gctttggatt aagcggaaaa
     2221 gctgctcggt atcagcgaca tcggttttat aaaatttacc atctgtcgat tgcattttca
     2281 gttggttaca atttgtaacc aactcacttc cctcctttac cagccgatgt ttaagaacct
     2341 tccagtaatt atttgggttg ggactttccg acaacactcc taccacatct acaatcgaga
     2401 agtaccattt ttcctgttcg tcgtcccaat gggtgcgaat ttgcttctca ttgaataatt
     2461 taatcgccgt ttctttggtc attttctatg tttttgaagg tttatattcg ttcttcaaca
     2521 cgtaactaat gaagttgtaa cttagaaaag tttcctttat aaatctcgaa aaagcagata
     2581 accttatcaa caagctgcct aacgaaataa ttccaatggg ttcattctta aaaataattc
     2641 taatgggatt ccacccgatc cgaccatcaa tacttttgct gaattatact tctgggcaaa
     2701 taaatccatt cctttattaa ttgtatttga accacttttc acctcaagag caacagtctt
     2761 accccgatgc tcaagcacaa aatcaacttc attgtttcct tcgcgccaat agtaaagttg
     2821 aaaacgctcc gaaaccgaat aattgatcaa atgtgcccca actgccgact cgactaaccg
     2881 ccc
//
LOCUS       DAOPUW010000210         2872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_36043_length_2872_cov_3.629393, whole genome shotgun sequence.
ACCESSION   DAOPUW010000210 DAOPUW010000000
VERSION     DAOPUW010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36043_length_2872_cov_3.629393"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PKJ43_04510"
     CDS             <1..273
                     /locus_tag="PKJ43_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71859.1"
                     /translation="LIKKASILLNLGETNSALSLINSLLLIEGTSPDLYLMKGTAHLV
                     LGDLNEAKDNFAKTLKYSFGTTKKTITTLAMPMNRPTISKKPCPIF"
     gene            258..1202
                     /locus_tag="PKJ43_04515"
     CDS             258..1202
                     /locus_tag="PKJ43_04515"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ71860.1"
                     /translation="MSYFLKAFALNEKNEMVLYEIAYCYEKTGDNYKSIEFYNKYLDI
                     DSFSDNAWFNLGILYNKLEKHKKAIEAYEFAVAINDDFASAWFNLGNSYMLRKNFSKA
                     IDAFTEFLRFDDSSDEICCIIGDCHINNRDHEKAFEFYQRAVTFNKKNDRAWFGSALA
                     MKLINDYHAAYKYLKKAVNIDYDNSEYWLFMAKICRKLNLRSETETAYERVCELNPGK
                     LTGWLKLSEHLHHNGMINKAISILETSLFYHPANAVLHYRLSAYYFESMNEKNAVKHL
                     KKALLVDADKQNYLFDILPEARLNETIVKLIDKYGNKS"
     gene            1242..1991
                     /locus_tag="PKJ43_04520"
     CDS             1242..1991
                     /locus_tag="PKJ43_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNZ71861.1"
                     /translation="MSTYGLIGYPLGHSFSKKFFTEKFEKEGLTNCKYLNFEIENIDM
                     FPTIFQQYNDIKGLNCTIPYKQQVMKFLDEVDEETAKVGAVNTIKPIYTANGVKLKGF
                     NTDIIGFERSLKPMLKQTHTKALILGTGGASKAIKHILQKLGIDFLSASIENELFKNE
                     IRYSDIDKKMIEERTIIINATPLGTHPKIDACPDIPYEYLTSDHVLFDLVYNPEVTLF
                     MKKGIEKGANVKNGLEMLHGQALAAWEIWNS"
     gene            2171..2497
                     /locus_tag="PKJ43_04525"
     CDS             2171..2497
                     /locus_tag="PKJ43_04525"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HNZ71862.1"
                     /translation="MRTDQEKYCFSCGEAIFSKAEICPKCGVPQPDIVRNKTLNSRWI
                     AAVLLCWILGVFGAHRFYLGRTGSGVLMLLTIGGLGIWYLIDLILVIVGQMRDSNGQY
                     VKAQIE"
     gene            2638..>2872
                     /locus_tag="PKJ43_04530"
     CDS             2638..>2872
                     /locus_tag="PKJ43_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71863.1"
                     /translation="MKHILLIGLITLTLSMSAQNNVLKINEMLPAKTEKMKNALNDAT
                     VDLKSQVKENGLLVIFSCNTCPFVVAWEDRYPMV"
BASE COUNT          977 a          590 c          512 g          793 t
ORIGIN      
        1 ctcattaaaa aagcttcgat tttgctaaat cttggcgaaa ccaattcagc attatcactc
       61 atcaacagtc tgctgctaat tgaaggtact agccccgact tatatttgat gaaaggtacc
      121 gcacatttag tccttggcga tttaaacgaa gcaaaagaca attttgccaa aacattaaaa
      181 tattcattcg gaacaacgaa gaaaactatt acaacattgg ctatgcctat gaacaggcca
      241 accatttcaa aaaagccctg tcctattttc taaaagcttt tgcgctgaac gaaaaaaacg
      301 aaatggtact ttacgaaatt gcatactgct acgaaaagac aggagacaat tacaaaagta
      361 tcgaattcta taacaaatat ttagacatag actcattctc agacaacgcc tggttcaatt
      421 tgggtatttt gtacaataaa ctcgaaaagc acaagaaagc tattgaagct tacgaatttg
      481 ccgtagccat aaacgatgac tttgccagtg cctggtttaa ccttggcaac agctacatgc
      541 tgcgtaagaa tttttcaaaa gccatcgacg ccttcactga atttctccga tttgatgact
      601 caagcgatga aatatgctgc atcattggcg attgccatat aaacaaccgc gatcacgaga
      661 aggctttcga gttttatcag cgagccgtta cgttcaataa gaagaacgac cgtgcatggt
      721 ttggctctgc acttgcaatg aagttaataa atgattatca cgctgcttat aaatacctca
      781 aaaaagcagt aaacatcgat tatgacaata gcgaatactg gctgttcatg gccaaaattt
      841 gcagaaaact aaacctgcga tctgaaactg aaactgctta cgagagagtt tgtgaactca
      901 accccggtaa gctaaccgga tggcttaaat tatcggaaca ccttcaccat aatggcatga
      961 taaataaggc aatttctatt cttgaaacca gcttgtttta ccaccctgcc aatgccgttt
     1021 tgcattaccg cctatctgcc tattatttcg aatcgatgaa cgaaaaaaat gcagttaagc
     1081 acctgaaaaa agcactactg gttgatgccg acaaacaaaa ctacctgttc gacattcttc
     1141 cagaggcaag acttaatgaa actattgtaa aacttatcga caagtacgga aacaaatcat
     1201 aagtataccc tgtctgtcga ttctaaatca atcatcaaaa tatgtcaact tacggattaa
     1261 ttggctaccc actcggacat tcattctcaa aaaaattttt tactgaaaag tttgaaaaag
     1321 aaggacttac caactgcaag tacctcaatt ttgagatcga gaatatcgac atgttcccaa
     1381 caatttttca acaatacaat gatattaaag ggctaaattg caccataccc tacaaacaac
     1441 aggtaatgaa attcctcgac gaagttgacg aagaaacagc caaagtaggt gcagtaaaca
     1501 ccataaaacc aatttatacc gccaatgggg taaaacttaa aggttttaat actgatatca
     1561 ttggttttga gcgctcacta aagcccatgc tgaaacaaac ccataccaaa gcgttaattt
     1621 tgggaaccgg tggagcatca aaagccatta aacatatttt acaaaaactg ggcatcgact
     1681 ttctttcagc atctattgaa aatgaactat ttaaaaacga aatacgctat tccgacatcg
     1741 ataagaaaat gatagaagaa cgtaccatta ttattaatgc taccccactg ggaacgcatc
     1801 ccaaaataga cgcctgcccc gatatcccat acgaatattt aacttctgac cacgtgttat
     1861 tcgatttggt ttataaccct gaagtaacat tattcatgaa gaaaggaatt gaaaaaggtg
     1921 ccaatgtgaa aaatgggctg gaaatgcttc atggacaagc tcttgcagct tgggagatat
     1981 ggaacagcta agccggttct ataacctgtt ccgcagataa atttctgaaa tcaaagagct
     2041 catcaaattc aattatttgg ttggtagccg cccaaagatg tgttgttcac cgataaaata
     2101 agacagttta tccaaaacaa tttatacgtt tttagcaagc tgccgtacat ttgagaaaaa
     2161 attaaacatc atgagaacag atcaagaaaa atattgtttt agctgcggag aagccatctt
     2221 ttcaaaagcc gaaatttgcc ccaaatgcgg agttccccaa ccggatatcg ttagaaacaa
     2281 aacacttaat agtcgatgga ttgccgctgt tctcttgtgt tggatccttg gcgtttttgg
     2341 agctcaccgg ttttatcttg gacgaactgg ttccggagtg ctgatgctat taacaattgg
     2401 cgggctcgga atctggtatt taatcgacct tattcttgta attgttggcc aaatgcgcga
     2461 tagtaatggc caatacgtta aagctcaaat tgaataaacc catatttaat ctgctaacaa
     2521 ggctgctccc attttggaag cggctttgtt tttttataca cctaaggcta cccgcatttt
     2581 gattgaacaa aacccacttc tccttgttta ttcaaactaa atttaaacaa ggaaaatatg
     2641 aaacatattc tgctaattgg gttaataact ctcaccttaa gtatgtcggc acaaaacaat
     2701 gtgctgaaaa ttaacgaaat gcttcctgca aaaactgaaa agatgaaaaa tgcgcttaat
     2761 gacgctactg ttgaccttaa atctcaggta aaagaaaacg gtcttctggt tatattctcg
     2821 tgtaacacct gcccttttgt tgttgcctgg gaagaccgct atccgatggt ga
//
LOCUS       DAOPUW010000211         2868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_36121_length_2868_cov_3.029506, whole genome shotgun sequence.
ACCESSION   DAOPUW010000211 DAOPUW010000000
VERSION     DAOPUW010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36121_length_2868_cov_3.029506"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..844)
                     /gene="hisF"
                     /locus_tag="PKJ43_04535"
     CDS             complement(89..844)
                     /gene="hisF"
                     /locus_tag="PKJ43_04535"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNZ71864.1"
                     /translation="MLAKRIIPCLDIKDGQTVKGINFVGIKAVGDPVELGALYAEQGA
                     GELVFLDITATHEGRKTFVDLVKRIAREINIPFTVGGGISELKDAEALLKAGADKISI
                     NSSAVRNPKLIDEMALNFGTQFVVVAIDAKKYDDRWTVTVNGGRIPTEKELFSWAKEA
                     EDRGAGEILFTSMDHDGTKNGFANEELAKMAGMLKIPIIASGGAGNMDHFVDVFTKGK
                     ADAGLAASIFHYKEIAIPDLKNYLSSKNIPVRI"
     gene            complement(1050..1412)
                     /locus_tag="PKJ43_04540"
     CDS             complement(1050..1412)
                     /locus_tag="PKJ43_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNZ71865.1"
                     /translation="MQSYRDLIVWQKSMSLVMMLYGATKGFPKEEAFGLTSQIQRSAV
                     SIPSNIAEGYGRNSTGDYARFLQIASGSLYEFQTQLEISFQLGYLNNERFNEINSLSI
                     EIEKVLSSLISKVKNSKK"
     gene            complement(1487..2212)
                     /gene="hisA"
                     /locus_tag="PKJ43_04545"
     CDS             complement(1487..2212)
                     /gene="hisA"
                     /locus_tag="PKJ43_04545"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNZ71866.1"
                     /translation="MITIIPAIDLIDAKCVRLSQGDYNQKTVYNENPLEVAKMFEGAG
                     IKRLHLVDLDGAKAKHIVNYKVLETIASKTKLVIDFGGGLKSDEDLRIAFESGASMVT
                     GGSIAVKDRETFLHWIETYGAEKIILGADAKDKMIAVSGWQEVSELPILEFVESYTSK
                     GIQKVISTDIARDGMLTGPSIDLYKEIMVKFPNLELIASGGIATMKDIYELDEMGVPG
                     VITGKAIYENRISLDEISKFISK"
     gene            complement(2209..>2868)
                     /locus_tag="PKJ43_04550"
     CDS             complement(2209..>2868)
                     /locus_tag="PKJ43_04550"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNZ71867.1"
                     /translation="AYVADMAKYATPDETTRIMAAFNTIPAQLAKENRKFQYKLIKTG
                     ARAHEFETALDWLQASGVANKCVKITEGKFPLQLYAENAAFKIYLSDNGLLCSKFGIA
                     PHFLISGNRIIDNIKGALTENFVMNSLVVNNFQPNYWESQGKAEVDFVVQMNDGKVIP
                     IEVKSSENVRSKSLKQFIDKYNPAYSIRVSTKNFGFENGIKSIPLYALHCFTTDYNLD
                     I"
BASE COUNT          721 a          648 c          553 g          946 t
ORIGIN      
        1 ttttatttaa acacaaagac acgaagtatt tcacaaaggg acacaaagat ttatttttct
       61 ttgtgacttt gcgacttcgt attttcactt atattcttac tggtatattc ttacttgaca
      121 aatagttctt taaatcagga atggcaattt ccttgtagtg gaaaatgctg gcagccaatc
      181 cggcgtcggc tttgcctttg gtgaacacat caacgaagtg atccatattc cctgcgccgc
      241 ccgaagcaat gatgggtatt ttcaacatac cggccatttt ggcgagctct tcgtttgcaa
      301 atccgttttt ggtgccatca tgatccatgc tggtgaaaag gatttcgcct gcaccacggt
      361 cttcggcttc cttggcccaa ctgaacaatt ccttttccgt gggaatacgt ccgccattta
      421 cagtaaccgt ccagcggtcg tcatatttct tggcatcgat ggctaccact acgaactgtg
      481 tcccgaagtt cagtgccatc tcatcaatca gcttagggtt tcgtaccgcc gaagaattga
      541 ttgagatctt atctgctcca gctttcaata gggcttcggc atccttaagc tcactaatcc
      601 ccccccctac ggtaaaggga atgttgatct cgcgggcaat gcgcttaacc aaatcgacaa
      661 aggttttgcg gccttcgtgg gttgcagtaa tgtcgaggaa aaccagttca ccggccccct
      721 gttcggcata cagcgcgccc aattcaacgg gatcgcccac ggctttgatc ccaacaaagt
      781 tgatgccttt cacggtctga ccgtctttga tatcaaggca aggtatgatt ctttttgcta
      841 acatattttt ttgtttaaca gttgtcaggc agaagttaga agttaaaact gcggctcgta
      901 ttctagttta acagtttaac aggcagtagt tagaaggcag aaggtaaagg tgtcattcat
      961 taaaatattt ttacacttct gccttctgcc tgtttaactt ctgccttctg cctgtttaac
     1021 ttctcacttc tatttttctt ctgcctgttt tacttcttac tatttttcac cttcgaaatt
     1081 agacttgaaa gcaccttctc aatttcaata cttagcgagt ttatttcatt aaaacgttcg
     1141 ttattcaagt aacctaattg aaaacttatt tctaattgtg tctgaaattc ataaagcgac
     1201 ccagaagcga tttgaagaaa ccgagcgtaa tctcctgtag aatttcttcc atagccttca
     1261 gcaatattgc tcggaataga aacagcactt cgctgaattt gcgatgtcag tccaaaagct
     1321 tcttcctttg ggaaaccttt agtagcacca taaagcatca ttactagtga catcgatttt
     1381 tgccaaacga tcaaatccct atacgattgc atattcttga gtttttatat tgcattttac
     1441 ttcttacttc ttcctgttca acttctttct tctgcctgtg aacttcttac ttagaaataa
     1501 acttcgagat ttcatccaac gatattcggt tttcataaat tgcttttcct gttattactc
     1561 ccggaacacc catttcgtcg agttcgtaaa tgtctttcat ggtcgcaatt ccaccgctgg
     1621 caatcagttc gagatttgga aattttacca tgatttcttt atacaaatcg atacttgggc
     1681 cggtgagcat tccatcgcgg gcaatgtcgg tcgagattac tttctgtatg cctttgctgg
     1741 tgtagctttc tacaaattct aaaattggca gttccgacac ttcctgccaa ccactcactg
     1801 caatcatttt atctttggca tcggctccga ggattatttt ttcggctccg taggtttcga
     1861 tccaatgcag aaaggtttca cggtctttca cggcaatact tcctccagtg accatgcttg
     1921 cgccactttc gaaggcaatg cgcaggtctt cgtcgctttt caatccgccg ccaaagtcaa
     1981 tcaccagttt ggttttcgat gcaatggttt ctagcacttt gtaattcaca atgtgtttag
     2041 cttttgcacc gtcgagatca acgagatgaa gccgtttgat accggcacct tcaaacattt
     2101 tggccacttc gagcgggttc tcgttgtaca ccgttttctg gttgtagtcg ccctgcgaaa
     2161 ggcgtacgca cttggcatcg attaagtcga ttgctggaat gattgtaatc atatatctaa
     2221 attgtaatca gtagtaaaac aatgtaatgc gtaaagaggg attgatttta tcccattttc
     2281 aaatccgaaa tttttggtcg aaacgcgaat ggaatatgcc ggattgtatt tgtcaatgaa
     2341 ttgtttaagg ctttttgatc gcacattttc cgatgatttc acctcaattg gaatcacttt
     2401 tccatcgttc atttgtacca caaaatcaac ttctgccttt ccttgcgatt cccagtagtt
     2461 tggttgaaag ttattgacta caagagaatt cattacgaag ttttcagtta aggcaccttt
     2521 gatgttgtcg ataatgcgat tgcccgaaat taggaaatga ggcgcaatcc caaatttaga
     2581 gcacaacaaa ccattatctg ataagtatat tttgaaagca gcattctcgg catacaattg
     2641 taatgggaac ttcccttcgg taattttaac acatttattt gcaacacccg aagcttgcaa
     2701 ccaatccaag gccgtttcga actcgtgtgc acgtgccccg gttttaatta atttatactg
     2761 gaatttacgg ttttcttttg ctaactgggc tggaattgta ttaaaggcag ccatgatccg
     2821 agtggtttcg tcgggtgtgg catatttggc catatccgct acgtatgc
//
LOCUS       DAOPUW010000212         2865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_36182_length_2865_cov_2.509253, whole genome shotgun sequence.
ACCESSION   DAOPUW010000212 DAOPUW010000000
VERSION     DAOPUW010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36182_length_2865_cov_2.509253"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="PKJ43_04555"
     CDS             complement(<1..954)
                     /locus_tag="PKJ43_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNZ71868.1"
                     /translation="MSFESEIERRKTFAIVSHPDAGKTTLTEKLLLFGGAIHIAGAVK
                     SNKIKKTATSDFMEIERQRGISVATSVMAFDYSGYKVNILDTPGHQDFQEDTFRTLTA
                     VDSVIIVIDAAKGVEPQTEKLMNVCRMRKTPVIVFINKMDRPSEDTFYLLEEIEKQLQ
                     INTRPLSWPINSGPDFKGVYNIFNSSLQLFNPNVQEIDPGIKFENLNDPELEKQIGKK
                     DADTLREELELLEGVYPEFDVNEYLEGNLAPVFFGSALYNFGVQELLDTFLKIAPTPR
                     PRSSQERLIDPYEQAFSGFVFKIHANIDPNHRSRIAFVKVCS"
     gene            1199..2242
                     /gene="porQ"
                     /locus_tag="PKJ43_04560"
     CDS             1199..2242
                     /gene="porQ"
                     /locus_tag="PKJ43_04560"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HNZ71869.1"
                     /translation="MRRLSILIVFLAIFNSAFSQFGGSNTYDFLNLASSARVNALGGY
                     QVGIVDSNELSLSYYNPSLLMPAAHEHLTLNYIRYIAGINVGYVSYARNVEKIGTFAA
                     GIHYVNYGKFDEALENGYLTGKTFSASDYALNLIFSRNIWKTFTAGVNVKPILSSYET
                     YTSLGIAADVGISYVDSSGLFSAGLVFKNMGTQITTYQYLTDGEYEPLPFDIQVGFSQ
                     KLAHAPLRFNVTLNQLHKWNLTDKKTWEYDHKDSTDYIEGSSDDFLRQTLRHVIFGVE
                     FIPSQNFSIGFGYNYQRMRELSISARQAAVGLSGGINVKISKFRLSYAIARYHLSGTS
                     NTFSVSFNLSEFR"
     gene            2302..>2865
                     /gene="cmk"
                     /locus_tag="PKJ43_04565"
     CDS             2302..>2865
                     /gene="cmk"
                     /locus_tag="PKJ43_04565"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNZ71870.1"
                     /translation="MNKKIIIAIDGHSSCGKSTVARQVAHALQYNYVDSGSMYRAVTL
                     FALRNKLIINGLVDSNRLVEMLPLISISFKYNPEEQKSDTYLNGENVEKEIRGLEVSN
                     NVSLVAVVKEVRQAMVKIQQEMGREKGIVMDGRDIGTVVYPQAELKIFMTTSPQIRAQ
                     RRYDELVGKGEAVSFDEILKNVEERDFI"
BASE COUNT          795 a          531 c          599 g          940 t
ORIGIN      
        1 cgagcatact ttcacaaatg caattcggct tcggtggttt gggtctatat tggcatgtat
       61 cttaaacaca aaaccgctga aagcctgttc atatggatct attaatcttt cttgagatga
      121 gcggggccgt ggggtagggg caatttttaa gaaggtgtca agaagctcct gaacaccaaa
      181 attatacaag gcactaccaa agaataccgg ggcaagattc ccttcaagat attcatttac
      241 atcgaattca gggtaaacac cctcgagcag ctccagctct tcacgcaatg tgtcggcatc
      301 ctttttccca atttgttttt caagctcagg gtcattaaga ttttcaaatt taattcccgg
      361 gtcaatctcc tgcacattgg gattgaaaag ttgtaaactg gaattaaaaa tattataaac
      421 acctttgaaa tcggggccgc tgttaattgg ccagctcaaa gggcgagtat tgatttgtag
      481 ttgtttttcg atttcttcga gcaaatagaa ggtgtcttcc gatggccggt ccatcttgtt
      541 aatgaataca ataacggggg ttttgcgcat tcggcaaaca ttcattaatt tttccgtctg
      601 aggttctacg cctttggctg catcaataac aataatgaca ctatcgacag ctgtgagtgt
      661 tctgaaagta tcttcctgaa aatcctggtg tccgggggtg tcgagaatgt ttactttgta
      721 gccgctgtag tcgaacgcca taaccgatgt ggccaccgaa attcctctct gccgttcaat
      781 ctccataaag tcagaggtag ctgttttttt tattttgttg cttttaacgg ctccggcaat
      841 atgaattgct cccccgaaaa gcagtagctt ttctgttaaa gttgtttttc ccgcatcggg
      901 gtgactgaca atggcaaagg tttttcgtct ttctatctcg ctttcgaaac tcattttcta
      961 tcgttttaaa atggctgcaa aaatagtgta tctatccgat aattaaaatt gaatttgaat
     1021 tcaattggaa gaggacaaga tttcatatct tttcctttga ttatcatttt attcttttga
     1081 attttttgtg gattttgatt ctgctttttc aggtattctc cataattttt tgatggctat
     1141 tctctggcat cggaataata aaaatttatc tttggcgtta tttcgtaaat aacataggat
     1201 gcgccgactt tcaattttaa tcgtgtttct agcgatattc aatagtgctt tttctcagtt
     1261 tggagggagc aacacatatg atttcctaaa tttagcttca tcggcaaggg ttaatgccct
     1321 cggtggctat caagtcggta tcgttgatag taacgagttg agtttaagtt attataaccc
     1381 gtcgttgttg atgccggctg ctcacgaaca tttaaccctg aattatataa gatatattgc
     1441 aggtattaat gtgggatatg tttcttatgc ccgtaatgtg gaaaaaattg gaacatttgc
     1501 ggcaggaatc cactatgtca attatggtaa gtttgatgaa gcactcgaga atggatacct
     1561 aaccgggaaa acattttctg cctccgacta tgccttgaat cttatctttt cccggaatat
     1621 ctggaaaaca tttaccgccg gagttaacgt taaaccaatt ctttcttcgt acgaaactta
     1681 tacttcgctt ggcattgcag ccgatgtcgg gatttcttat gtcgactcgt caggattatt
     1741 ctcggcgggt ttggtattca aaaatatggg aacccaaatt acaacttatc aatatcttac
     1801 cgatggagaa tatgagcctc tccctttcga tattcaagta gggttctcgc agaaattagc
     1861 gcatgctcca cttaggttca atgttacgtt gaatcaactt cataaatgga acttaaccga
     1921 taaaaaaacg tgggaatacg accataaaga ttcaaccgat tacattgaag gttcatctga
     1981 tgatttttta cgtcaaacgt tgcgccacgt aatttttggg gttgaattta ttccttctca
     2041 aaatttttcg attggatttg gttataatta ccaacgaatg agggaattgt ctatttcggc
     2101 acgacaagca gcggtcggtc tgtctggagg tattaatgtg aaaatttcaa aattcaggtt
     2161 gtcgtatgct attgcccgat atcaccttag cggtacttca aatacttttt cagtaagttt
     2221 caatttatca gagttcaggt aagcgttgct tcttttttcc tgactcattt caaagcgtta
     2281 tatttgcgct aaaattggtt catgaacaag aaaatcataa ttgctatcga tgggcattca
     2341 tcttgcggga aaagtaccgt tgcccgtcag gttgcccatg cccttcagta caattatgtc
     2401 gattcaggtt cgatgtatag ggctgtaact ctttttgctc ttcgtaataa attaataatt
     2461 aatggattgg ttgattctaa tcgtttggtc gaaatgcttc cgttaatttc aatttctttt
     2521 aagtataatc ctgaagaaca gaaaagcgac acatacctga atggcgaaaa tgtagaaaaa
     2581 gaaatccggg gtctcgaagt atcgaacaat gttagccttg ttgccgttgt taaggaagtt
     2641 cggcaagcaa tggtgaaaat tcagcaggaa atgggacgcg aaaaagggat tgttatggat
     2701 gggcgcgata ttgggacagt ggtttaccca caagcagaac taaaaatatt tatgactaca
     2761 tcgcctcaaa ttagagctca gcgcaggtac gacgaattgg ttggtaaagg agaagcagtt
     2821 tctttcgacg aaattttgaa gaatgttgaa gaacgcgatt ttatc
//
LOCUS       DAOPUW010000213         2858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_36307_length_2858_cov_2.682483, whole genome shotgun sequence.
ACCESSION   DAOPUW010000213 DAOPUW010000000
VERSION     DAOPUW010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36307_length_2858_cov_2.682483"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..935)
                     /gene="murI"
                     /locus_tag="PKJ43_04570"
     CDS             complement(108..935)
                     /gene="murI"
                     /locus_tag="PKJ43_04570"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNZ71871.1"
                     /translation="MKRNHPIGIFDSGYGGLTILKEIRKLLPQYDYIYLGDNARTPYG
                     TRSFDVVFEYTLEAVKKLFELNCELTILACNTASAKALRNIQQNILPEIAPEKRVLGV
                     IRPSVEEVLNITRNKHIGVLGTNGTVSSGSYLIELEKISNGELVVTQEACPMWVPIVE
                     NNEIGTMGANYFVEKNITQLLSSDPEIDTIILGCTHYPLLSETIRKYLPDGITLVEQG
                     PIVAKKMLDYLNRHPEMEEKCSAEGSIQYFTTEQASNFEGKASIFMDEKIEASTIRL"
     gene            complement(1022..1534)
                     /locus_tag="PKJ43_04575"
     CDS             complement(1022..1534)
                     /locus_tag="PKJ43_04575"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNZ71872.1"
                     /translation="MKKFFVTFLTIAALTITAAQAQTPKFGHIDIQQLIQVMPERTTA
                     VSELEKTAGELEEMLGTLQTELQTMYKEYTEKRESMSDLVRQAKEEDIQAKQQRIETF
                     RMQSEQQLQQKQQDLMKPIIEKADNAIAEVAKEQGLVYVFDVSSRVVLYKSNQSVDCL
                     PLVKKKLGIE"
     gene            complement(1649..2164)
                     /locus_tag="PKJ43_04580"
     CDS             complement(1649..2164)
                     /locus_tag="PKJ43_04580"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNZ71873.1"
                     /translation="MKRIIITFIAVAVLSIASYAQKYAFVDTEYILGNIPAYKAAQEQ
                     LDQLSNQYQKELEGIQAELEQMYNDFRAESVLLSDDLKRKREDVIVTREKEYRTLQQK
                     YFGREGELFKKRQSLVKPIQDDIYNTIKTLATEGNYAVIFDKSGGLTMLYTDPKYDLS
                     DQILEKMGYKN"
     gene            complement(2224..>2858)
                     /locus_tag="PKJ43_04585"
     CDS             complement(2224..>2858)
                     /locus_tag="PKJ43_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNZ71874.1"
                     /translation="SLSLELTPPYSSFNGEDYADPNMSDKKRFSFLEYHKWLFNAKWF
                     TTLDRNEKFVLHAKYEFGLLGYFDEHRKSPLEGFKMGGDGMSGYDFYGSDNIALRGYA
                     NGSITPTSGNGRTGNIYAKTTIELRYPITLSESANIYALSFLEAGNSWYDFNTFNVFD
                     LKRSAGVGVRFWLPMFGLLGIDWGYGFDKPNNGSASSAKSQFHFVIGQQF"
BASE COUNT          738 a          579 c          587 g          954 t
ORIGIN      
        1 accagaccaa tgtcttggtt gcctcgatgc ccggctttca ttggtgaata gcaaaattgg
       61 gtatggtgaa tggggcgact ctcatcattt ttcgctcaga aaaaatacta aagacggata
      121 gtagaggctt ctattttctc gtccataaaa atagacgctt ttccctcgaa atttgatgct
      181 tgttctgttg tgaaatattg aatactacct tctgccgagc atttttcttc catttcaggg
      241 tgccgattaa gataatcgag catttttttt gcaacgatcg ggccttgttc taccagcgtg
      301 atcccatcgg gtaagtattt tcgaattgtt tccgagagca acggataatg cgtacaacca
      361 agtataatgg tgtcaatttc aggatcagaa gacaataatt gagtaatgtt tttttcgacg
      421 aaataattgg ctcccatggt gccaatttca ttgttttcga caataggcac ccacattggg
      481 caggcttcct gagttacaac aagttctccg tttgatattt tctcgagttc gatgagatat
      541 gatcccgacg aaactgtgcc gttggttccc aacactccaa tatgcttgtt gcgggttatg
      601 tttaagactt cttcaacact tggccgaata accccaagta ctcttttctc aggggcaatt
      661 tccggaagga tattctgctg aatgtttcgt aaggctttag ccgatgcagt gttgcaggca
      721 agaatagtca gctcgcagtt caactcaaat agttttttca ctgcttcgag ggtatattca
      781 aaaaccacgt cgaatgagcg cgttccatat ggagtacggg cattgtcgcc cagataaata
      841 taatcgtatt gaggcagtaa cttccttatt tcctttagga tggtcaatcc tccatacccc
      901 gagtcaaata ttccaattgg atggtttcgt ttcattgtta tgcgttattg ggtactaaag
      961 tagcaaaaga gccgcttgaa aaatcaaacg gctctcacta tttttttgaa ggtttaaaac
     1021 cttattcaat tccaagtttc tttttaacta atggaaggca atcaacactc tggttcgatt
     1081 tgtaaagcac aactcttgaa cttacatcaa aaacataaac aaggccttgt tcttttgcaa
     1141 cttcggcaat agcattgtca gctttttcga tgataggttt catcaaatcc tgttgttttt
     1201 gttgtagttg ttgttccgat tgcattctga atgtttcaat cctttgttgt tttgcctgaa
     1261 tatcttcttc tttggcctga cgaaccaagt ccgacataga ttcacgtttc tcagtatatt
     1321 ctttgtacat ggtttgaagt tcagtttgca acgttccaag catttcttcg agttcgcctg
     1381 cagttttttc aagttctgaa actgcggtag ttctttcagg cattacttga ataagttgtt
     1441 gaatgtcaat atgtccaaat tttggagtct gagcctgagc agcagtaatt gtaagtgctg
     1501 caatagtaag aaatgttaca aaaaactttt tcattttatt tctattaatt gttttcgttt
     1561 aaataagcca cgcaaatata aaaattaact tcgatttacc ccattatttg aacatgttag
     1621 ttcgaattgt gataagtgca ggaatttttt agtttttata tcccattttt tcaaggatct
     1681 gatcgctcaa atcgtatttt ggatcggtgt aaagcatggt caagccaccc gatttgtcga
     1741 aaatgacagc atagtttcct tcggtagcaa gggttttaat cgtgttgtaa atatcatctt
     1801 gaattggttt taccaggctc tggcgctttt tgaatagttc tccttcgcgg ccaaagtatt
     1861 tttgctgaag tgtacggtat tctttttcgc gggtaacaat tacatcttcg cgtttgcgtt
     1921 tcaaatcgtc ggaaaggagt accgattcag ccctgaagtc gttgtacatt tgttcgagct
     1981 ctgcctgaat tccttccagt tctttttgat actggttcga caattggtcg agttgttctt
     2041 gggctgcttt gtaggccggg atgtttccga ggatatattc tgtatcaacg aaagcatatt
     2101 tctgcgcgta actagcgatc gataaaaccg ctacggcaat gaatgtgatg ataatacgtt
     2161 tcataatctt tacgtttttt aatttcttta tgtgttacaa ctttcgtacc aagatatctt
     2221 caattaaaat tgctgaccaa ttacgaagtg gaattgactt ttggccgacg aagctgaacc
     2281 attgtttggt ttgtcgaaac catatcccca gtcaattccc agcaaaccga acattggcaa
     2341 ccaaaacctt actccaacac cggccgaccg cttcaggtcg aacacgttaa aggtattgaa
     2401 atcgtaccag ctgttaccgg cttcgagaaa cgatagtgca tatatgtttg ccgactctga
     2461 caatgtaatc gggtagcgca attcaatggt tgtttttgcg tagatatttc cggtacgccc
     2521 gttgcccgac gttggggtta tacttccgtt tgcgtatcct ctgagagcga tgttgtcgga
     2581 gccgtagaag tcatatcccg acattccgtc tccccccatc ttaaagcctt cgagtggcga
     2641 tttccggtgt tcatcgaaat atcccagtaa cccgaattca tatttggcat gtaaaacaaa
     2701 cttttcgttt ctgtcgagtg tagtgaacca cttcgcattg aaaagccact tgtggtattc
     2761 aaggaagcta aaacgctttt tgtcgctcat attcgggtcg gcataatctt ctccgttgaa
     2821 agaggagtag gggggggtta actcaagcga taaactga
//
LOCUS       DAOPUW010000214         2847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_36486_length_2847_cov_3.558381, whole genome shotgun sequence.
ACCESSION   DAOPUW010000214 DAOPUW010000000
VERSION     DAOPUW010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36486_length_2847_cov_3.558381"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1556
                     /locus_tag="PKJ43_04590"
     CDS             <1..1556
                     /locus_tag="PKJ43_04590"
                     /inference="COORDINATES: protein motif:HMM:NF014466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HNZ71875.1"
                     /translation="IVALSFLVTISYAQNTDRNWGLGVLAGNVQNLAVSDNDIAFHFY
                     LNHYLSSSFDVQFKQTFSFFASLPDTSLWLDNSNTILGMRFKFNNGTLLPVNNRFKPF
                     LSLGVGYLYDNDTGGINFDADVGAKYGIKSGLSLVAKIGYIHGIDSKVGKSTRHDNFL
                     RVSVGVEFAFGRPADSDKDGVPDSRDNCPGTESGLKVDKFGCLLDSDGDGISDVYDKC
                     PKLKGDISFDGCPDSDEDGIPDNDDLCPLQAGMVKFMGCPDSDQDGVSDDRDRCPEVA
                     GAAKLWGCPDRDDDGVIDIDDECPDDAGTILTKGCPDRDSDGIANSVDECPNEFGHKM
                     LNGCPDDDEDGVANKNDLCTDTPVGSVVDSLGCPLDSDNDGIFDGIDRCPDVIGILKN
                     NGCPLDKKKVEWINDYELQSVGFSKESAEITREMRQALAMLITILLNDSEYYAHITTH
                     VPVTSSADSNLSFTETKMDALVEYLVSKGISKSRISTEILTKKIQTKKYGEENIETER
                     QIFDFKIYR"
     gene            1698..2492
                     /locus_tag="PKJ43_04595"
     CDS             1698..2492
                     /locus_tag="PKJ43_04595"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HNZ71876.1"
                     /translation="MMKSIHNQLVVVVFLLAVVLSSCSSTKKIASYNAQNISVDKQID
                     TVQDISYLSIIQPYTDKLASELTGIVAIADTSLIAYRPESPLSNFISDLLLAWGKTFA
                     KTNQPTISIDFSLVNIGGLRTSLPQGEIQVRNIFELLPFENELVFVQLKGNQVNILLD
                     HLASRDGEGVSGTTFGIKKGKAVEVTVGGNPVDNNKLYWVLTNDYVANGGDGMKVLTE
                     AVQRVDTGEKIRDVVISELKEMNQKGIRINAKNDGRVYYVEQANIH"
     gene            2449..>2847
                     /locus_tag="PKJ43_04600"
     CDS             2449..>2847
                     /locus_tag="PKJ43_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ71877.1"
                     /translation="MTGGCTMWNRRTFIKTVAAGGLGFAAMPQMVLAGNNTQRLLILH
                     TNDIHCRFEPFPETDPKYAGKGGLNRISAYVKAMRQRNPDLLLFDCGDFSQGTPYFNF
                     FKSEVVLKLMSEMKYDAGTLGNHEFDNGLQG"
BASE COUNT          833 a          513 c          680 g          821 t
ORIGIN      
        1 ccatagtagc attgtcgttt ctggtaacta tttcgtatgc ccagaatact gataggaact
       61 ggggacttgg tgttttggcc ggcaatgttc aaaatcttgc agtgagtgat aatgatattg
      121 cttttcattt ctacctgaac cattacctct cttcttcctt cgatgtgcag tttaagcaaa
      181 cattcagctt ttttgccagt cttcccgata cctcactttg gctcgataac tcgaacacaa
      241 tcttaggcat gcggtttaaa tttaacaatg gaactttatt gcctgtaaat aaccgtttta
      301 aaccatttct ttcgctaggg gtaggctatt tgtacgataa tgacactggc ggaataaact
      361 ttgatgctga tgtaggagct aaatatggaa taaaaagcgg gttgtcgctg gtggccaaaa
      421 taggatatat acacggaatt gattcgaaag ttgggaaaag cactcgacac gataattttc
      481 tgcgagttag tgttggtgtt gaatttgcgt ttggtcggcc tgcagactca gataaagacg
      541 gagtacccga ttctcgcgat aattgtccag gcactgaatc tggtttaaaa gttgataagt
      601 ttggttgttt gctcgactca gatggcgacg ggataagcga tgtctatgat aaatgcccga
      661 aattaaaagg ggatatttct ttcgatggtt gccccgattc tgatgaagac ggtattcccg
      721 ataatgatga cctttgccca ttacaggcag gaatggttaa atttatgggc tgtcccgaca
      781 gtgatcagga tggcgtgagc gatgacagag accgatgccc ggaagttgca ggagcagcta
      841 agctttgggg gtgtcccgat agggatgatg acggcgttat cgatattgat gatgaatgtc
      901 ccgatgatgc cggaactatt cttaccaaag gttgtcccga tcgtgatagc gacggaattg
      961 caaactcagt cgatgaatgt ccaaatgaat ttggccacaa aatgttgaat ggctgcccag
     1021 atgatgacga ggatggtgtt gccaacaaaa acgacttgtg tactgatacc ccagtaggtt
     1081 cagttgttga ttcgttagga tgccctttag acagcgataa tgatggaatt tttgacggca
     1141 ttgaccgctg tcctgacgta atagggatat tgaaaaataa cgggtgccca cttgataaga
     1201 aaaaagtaga atggataaat gactacgaac ttcaatctgt gggctttagc aaagagagtg
     1261 cagaaattac ccgcgaaatg cggcaagcat tggccatgct tattactatt cttttaaatg
     1321 attcagagta ttatgctcat ataacgactc atgtgcctgt aacatcttct gccgattcta
     1381 acctttcatt tactgaaacc aaaatggatg cgctggtaga gtaccttgtt tctaaaggca
     1441 tttctaaaag caggatatct acagaaattc tgacaaaaaa gatacagaca aaaaaatatg
     1501 gtgaagagaa catagaaacc gaaagacaaa tttttgattt caagatatat aggtagatat
     1561 tgtttaagaa ggttttatcg ttagcgatca tcccgctttt ttattctatg gcaattctga
     1621 aatcgtactt tgctgcacct aaaaaaaata tatcttagct tccgaaatga attgaaagtg
     1681 aatcatacga ttaagatatg atgaaaagca tacataatca attagttgtt gtggtatttt
     1741 tgttggcggt tgtattatcg tcgtgttcgt caacaaaaaa aatagcctcc tataacgcgc
     1801 agaatatttc agtcgataag caaatcgata ccgttcagga tattagctat ttgtcaataa
     1861 tacagccgta tactgacaaa ttggccagtg agttaacagg gattgtggcc attgccgaca
     1921 cttcgctgat tgcttacagg cccgaaagcc cattaagtaa ctttatcagc gacctgttac
     1981 tggcgtgggg caagaccttt gcaaaaacca accagccaac catttcgatc gatttttcgc
     2041 tggtaaacat aggcggcttg cgcacttctt tacctcaggg cgagattcag gtaaggaata
     2101 tctttgaact tctgccgttc gaaaatgaat tggtttttgt gcaattaaaa ggaaatcaag
     2161 taaatatttt gctagatcat ttagcgtcgc gagatggtga aggcgtttcg ggtactacct
     2221 tcggaattaa aaaaggaaaa gcagttgagg taacagttgg aggtaacccg gttgataata
     2281 ataagttata ttgggttcta accaacgatt atgtggccaa tgggggcgat ggaatgaagg
     2341 ttctgactga agccgtgcag cgtgtcgata ctggagagaa aatacgcgat gttgtgattt
     2401 ctgaattaaa agaaatgaac caaaagggaa ttcgtatcaa tgcaaaaaat gacgggaggg
     2461 tgtactatgt ggaacaggcg aacattcatt aaaaccgttg ctgccggggg gctgggtttt
     2521 gcagctatgc cacaaatggt gctggctggg aataatacgc agcgtttgct cattctgcat
     2581 accaacgaca ttcattgccg gtttgagcca tttcctgaaa ctgatccgaa atatgcagga
     2641 aagggcgggt tgaaccgtat ttctgcgtat gtgaaggcaa tgcggcagcg aaatcctgat
     2701 ttattgctct tcgattgtgg cgacttttcg cagggtaccc cgtatttcaa ttttttcaaa
     2761 agcgaggttg ttttaaagct gatgtcggaa atgaaatatg atgccggaac ccttggaaac
     2821 catgagttcg ataatggctt gcagggt
//
LOCUS       DAOPUW010000215         2845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_36514_length_2845_cov_3.408602, whole genome shotgun sequence.
ACCESSION   DAOPUW010000215 DAOPUW010000000
VERSION     DAOPUW010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36514_length_2845_cov_3.408602"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PKJ43_04605"
     CDS             complement(<1..490)
                     /locus_tag="PKJ43_04605"
                     /inference="COORDINATES: protein motif:HMM:NF025647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HNZ71878.1"
                     /translation="MELTYPNDNGSDLYPDIIESPKTNFGENAFLSSRIEVIFMAIFF
                     SLFLFSCSKPGQVTIKGTITNSLTNKVYLDKLGINETSPFDSTTIGKDGVFTLKSMVD
                     QPTFFLLRLGDQRFITLLLDSAERVVLSADFINFSSDYEIKGSSGSEKVLLLNRHLMR
                     TNA"
     gene            681..1622
                     /locus_tag="PKJ43_04610"
     CDS             681..1622
                     /locus_tag="PKJ43_04610"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNZ71879.1"
                     /translation="MLIHHDINLFKATNPVITIGTFDGVHLGHKQVISRLNNLARNVQ
                     GESVIFTFYPHPRLVVSAHESNLRLLTTFEEKARLLEEAGIDHLVVYPFTIDFAELTY
                     EQFIREILIEKLGLHTLVIGHDHRLGKNREGTFDNVVALSQKLHFGVEKIETFLINDV
                     DISSSKVRNALQKGEIEKANEYLGYNYQLTGTVASGSQIGRNIGFPTANIVTADYSKL
                     IPDEGVYAVTVSFEGKTYNGMLNIGYRPTIDANADHRTIEVHIFEFEGDIYKKEITIK
                     FLKRIRDEKKFASIEDLRLQLIEDRKTVESLLPPPLN"
     gene            1742..>2845
                     /locus_tag="PKJ43_04615"
     CDS             1742..>2845
                     /locus_tag="PKJ43_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNZ71880.1"
                     /translation="MTLANEKTLPYKVADIALADFGRKEIEIAEKEMPGLMALRKKYG
                     TTKPLKGARIMGSLHMTIQTAVLIETLVELGAQVRWASCNIFSTQDHAAAAIAAAGIP
                     VFAWKGETLAEYWWCTERALDFGNGKGPTLIVDDGGDATLMVHLGCAAEKDSSLLDVK
                     TEAIDEIELYACLKKILAETPKRWSSVAAEIKGVSEETTTGVHRLYQMMEEGKLLFPA
                     INVNDSVTKSKFDNLYGCRESLADGIKRATDVMIAGKVVVVAGYGDVGKGCARSMRGY
                     GARVIVTEIDPICALQAAMEGFEVKPMEETLCEGNIYVTTTGNRDIITAEYMAAMKDQ
                     SIVCNIGHFDNEIQVSRLEKWEGIKKTNIKPQVD"
BASE COUNT          887 a          622 c          618 g          718 t
ORIGIN      
        1 aagcatttgt acgcatcagg tgccgattga gcaaaagaac cttttctgaa cctgatgacc
       61 cttttatttc ataatcagaa gagaaattga taaaatcggc cgaaaggaca acccgctctg
      121 cagagtcgag caaaagggta ataaacctct gatctccaag cctgagaagg aaaaaggtag
      181 gttgatcgac catactttta agggtaaaca cgccatcttt tccaatggta gttgaatcga
      241 aaggagaagt ttcatttatg cccaatttgt cgaggtaaac cttatttgta agtgaatttg
      301 taatagtccc tttaattgta acctgacctg gctttgaaca agaaaataaa aataaactaa
      361 aaaaaatagc cataaaaata acttctatcc gactggataa gaatgcattc tcaccaaaat
      421 ttgttttggg gctttctata atgtcagggt aaagatcaga tccgttgtca ttaggatatg
      481 ttagttccat tatatgtcat ttaaacttgt taatcgcctt tgcatactat ttgccgctaa
      541 tatactaaat tagggttaag ccgtttcgct tataacaatt tttaagactt tacataaact
      601 ggatcgaaca aaagaattac ttttgcaggt agcattaatg caaggtaaaa ctcaaaatta
      661 aataattatc ggttatatct atgctaatac atcacgacat caacctgttc aaagccacca
      721 atccggtaat aactatcggc accttcgatg gcgtacatct ggggcataag caagttattt
      781 cacggttgaa caacctggcg cgcaacgttc agggcgagag tgtgatcttc acgttttacc
      841 cacacccacg gctggttgtt tcagctcacg agtcgaacct tcgcttgctt accacctttg
      901 aagaaaaggc tcgtttgctt gaagaagccg gaatagacca cttagtggtg taccctttca
      961 cgatcgactt tgccgaacta acttacgaac aattcattcg cgagatccta atcgaaaagc
     1021 taggtctcca cacgcttgtc atcgggcacg accatcgctt aggaaaaaac cgcgaaggta
     1081 ctttcgacaa tgtggttgcc ttatcgcaaa agctgcattt cggggttgaa aaaatagaga
     1141 cctttctgat taacgatgtc gatatcagct catcgaaagt gcgcaatgcc ctgcaaaaag
     1201 gtgaaataga aaaagccaat gaatatttgg gctataacta ccagcttaca ggtaccgttg
     1261 caagcggcag ccagataggc cgtaacattg gcttccccac agctaacatc gtaactgccg
     1321 attattcgaa actaatcccc gatgaagggg tatacgcggt aaccgtttca ttcgaaggga
     1381 aaacatacaa cgggatgctc aacatcgggt accggcctac tattgacgcc aatgccgacc
     1441 accggaccat tgaagtgcat atcttcgagt tcgaaggcga tatctataag aaagagataa
     1501 ccataaaatt cctaaaaaga attcgcgacg agaaaaagtt tgcttccatt gaggatctcc
     1561 ggcttcaact gattgaagac aggaaaaccg ttgaaagcct cttgcctcca ccgctgaatt
     1621 agctttgtaa ccctttcggc acaagacctt tctgcattat ttcaaagaaa tcaattgcat
     1681 aaaatgtgaa cttgccaatc tgcaagctat acactatctt tgcagcctaa tttttgtaaa
     1741 catgacatta gcaaacgaaa aaacattgcc atataaagtt gccgacattg cgttggccga
     1801 ttttggcaga aaagagattg aaatagccga gaaagaaatg ccggggctta tggccctccg
     1861 caaaaaatac ggcacgacca agccacttaa aggcgcccgg attatgggtt cgctgcacat
     1921 gaccattcaa accgccgtac ttattgaaac ccttgtagaa cttggagcac aggttcgttg
     1981 ggcaagctgc aatattttct caactcaaga ccatgcggca gcagccattg ccgctgccgg
     2041 gattcccgtt tttgcatgga aaggtgaaac gttggccgaa tattggtggt gcaccgaacg
     2101 tgccctcgat tttggaaacg gtaaagggcc taccctgatt gtagacgatg gaggtgacgc
     2161 tactttaatg gtacacctgg gatgtgctgc agaaaaagac tcatcgctgc ttgatgttaa
     2221 aacagaagcg attgacgaaa ttgaactgta tgcctgcctt aagaaaattt tagccgaaac
     2281 acccaagcgc tggagttcag ttgccgccga aataaaaggg gtatctgaag aaaccaccac
     2341 aggtgtacac cgcctctacc aaatgatgga agaaggaaag ttgttgttcc ccgccatcaa
     2401 cgtaaacgac tcggtgacca aatcgaaatt cgacaatctt tacgggtgcc gcgaatcgct
     2461 tgccgatggt attaaacgtg ccaccgacgt gatgattgcc ggaaaagttg ttgtggttgc
     2521 cggttacggc gatgttggaa aaggttgcgc ccgttcaatg cgtggatacg gcgcccgggt
     2581 tattgttacc gaaatcgatc caatttgcgc ccttcaggct gccatggaag gtttcgaagt
     2641 aaaaccgatg gaagaaaccc tttgcgaagg aaatatctat gtaacaacaa ccggaaaccg
     2701 cgacatcatt acagcagaat acatggctgc aatgaaagac caatcgattg tatgtaacat
     2761 tggccacttc gataatgaaa ttcaggtaag ccgtcttgaa aaatgggaag gaatcaaaaa
     2821 aaccaatatt aaacctcagg ttgac
//
LOCUS       DAOPUW010000216         2828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_36833_length_2828_cov_3.789758, whole genome shotgun sequence.
ACCESSION   DAOPUW010000216 DAOPUW010000000
VERSION     DAOPUW010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36833_length_2828_cov_3.789758"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PKJ43_04620"
     CDS             <1..594
                     /locus_tag="PKJ43_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71881.1"
                     /translation="AASQQTVFIALHPPVIMETLEKIAEVISEETELISLAPKITIEK
                     IASKVSSKYIARMIPNATSYINQGYNPIAFAPGMEKEDCKEIKKLLKPLGKIVETDES
                     KLEAYAITSAMLPTYFWFQIQELEKIAQQIGLTEEEASKTVKSTLKRAIKLYFESGLT
                     PAEVMDLIPVKPIGEHEAEIKEIYQTKLMGLFGKIKA"
     gene            643..1446
                     /locus_tag="PKJ43_04625"
     CDS             643..1446
                     /locus_tag="PKJ43_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNZ71882.1"
                     /translation="MKPEDLKLVVKEKYGQIAEQSKIQVQSSCCGSSGCCGELEFSMI
                     GDEYQYVEGYNPDADLGLGCGIPTEFAQIKKGDTVIDLGSGAGNDCFVARALVGETGK
                     VIGIDMTEAMIEKARENTKKLNYTNVEFRLGDIEKMPVTANKADVIISNCVLNLVPDK
                     RKAFSEIYRVLKPGAHLSVSDVVLKGELPEKIKQAAVMYAGCVSGAMQKDEYLNVISE
                     SGLVNVKVQKEKLINIPDEVLSNYLNQQEIDEFRKVGTGIFSITVYAEK"
     gene            1569..2624
                     /gene="arsB"
                     /locus_tag="PKJ43_04630"
     CDS             1569..2624
                     /gene="arsB"
                     /locus_tag="PKJ43_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNZ71883.1"
                     /translation="MKTRLKFLDRYLTLWIFLAMFAGVFMGWLSPKVAEFWNSMSSGT
                     TNIPIAIGLIVMMYPPLAKVKYEELGDVFKNTKILGLSLVQNWIIGPVLMFALAITFF
                     KIFPGENNANLPYMYGIIMIGLARCIAMVIVWNDLAKGDRQYAAGLVAFNSVFQVLLF
                     SVYAYLFIAILPGWFGVPVNETVSQITMGEIAKSVFIYLGIPMLAGFLTRFILTRVKS
                     KTWYETKFAPRISPLTLIALLFTIVVMFSLKGEYIVKIPMDVVLIAIPLLIYFVVMFF
                     ASFFMSKKVGATYEQATTLSFTAASNNFELAIAVAIAIFGINSGEAFAGVIGPLVEVP
                     VMIGLVNVAFWFKKKFY"
BASE COUNT          862 a          537 c          599 g          830 t
ORIGIN      
        1 gctgccagtc agcaaacggt gtttatagcc ctgcatcctc cggtaattat ggaaaccctc
       61 gaaaagatag cggaagtgat ttctgaagag actgaactga tttcgttggc gcctaaaatc
      121 accatcgaaa aaatagcttc gaaggtgagc agcaaataca ttgcacgcat gatacccaac
      181 gccacatcgt atattaacca aggctacaat cccattgcct ttgcacccgg tatggaaaag
      241 gaagactgta aggaaatcaa aaaactgttg aaaccgctgg gcaaaatcgt tgaaaccgac
      301 gagtcgaaac tcgaagccta cgccattact tcggctatgc tgccaactta tttctggttt
      361 caaattcagg aattggagaa aattgcacag caaattgggc tcaccgaaga ggaagcttcc
      421 aaaactgtta aatcgacatt gaagcgcgcc atcaaactct atttcgaatc gggcttaacg
      481 cctgccgaag taatggattt gattcctgta aaacctattg gcgaacacga agccgaaatc
      541 aaagaaattt accaaaccaa gttgatgggg ctgtttggga agattaaagc ctaaaatgga
      601 atcaaaaatt gaactattta ttgatcaaat taaacctaat ctatgaaacc cgaagatttg
      661 aagctggtcg taaaggaaaa atacggtcag attgctgaac aaagtaaaat tcaggttcaa
      721 tcatcctgtt gtggttcatc gggctgttgt ggagaattgg aatttagcat gattggcgat
      781 gaataccaat acgttgaagg ttacaaccct gatgctgatc tgggtttagg ctgtggtatt
      841 cctactgaat ttgcccagat caaaaaaggg gacacggtta tcgacttggg ttcaggtgcc
      901 ggaaacgact gctttgttgc ccgggctttg gttggtgaaa caggcaaagt aatcggtatc
      961 gatatgaccg aagccatgat tgaaaaagcc cgtgagaaca ctaaaaagct gaactacacc
     1021 aatgttgaat tccggctggg tgacattgaa aaaatgccgg taacagccaa caaagccgat
     1081 gtaatcatca gtaattgtgt tttaaatctt gtacctgata aacggaaagc cttcagtgaa
     1141 atttaccggg tattgaaacc cggtgcccat ctttcagtct ccgatgttgt tttaaaaggc
     1201 gaactgcccg aaaaaatcaa acaagctgcg gtaatgtatg ctggttgtgt ttcgggagca
     1261 atgcaaaaag atgaatacct gaacgtaatt agtgaaagcg gactcgtaaa tgtcaaagta
     1321 cagaaagaga aattaatcaa tattcccgat gaagttttgt cgaattactt aaaccaacag
     1381 gaaattgatg aatttaggaa agtcgggacg ggaattttca gcattactgt ttatgccgaa
     1441 aaataatttg aagattaagt gatttgaaga tttgaaaatg atagagatac aatcttcaaa
     1501 ttttcaaatc acttaatttt caaatatgtc ttcattttca aattgtttca tcttcaaatt
     1561 ttcaaatcat gaaaactcgt ttaaaattcc tcgaccgtta tcttactctc tggatattcc
     1621 ttgccatgtt tgcaggggtt ttcatgggat ggttaagccc taaagtagca gagttctgga
     1681 acagcatgtc aagtggtaca accaatattc caatagccat tggattgatt gtgatgatgt
     1741 atcctccact ggccaaggta aaatacgaag aactgggcga tgtctttaag aacacgaaaa
     1801 tccttggcct ttcattggtg caaaactgga ttattggtcc tgttctcatg tttgcattgg
     1861 ccataacctt ctttaagatt tttcccggtg aaaacaatgc caatcttcct tacatgtacg
     1921 gcattatcat gattggcctt gcccgttgta ttgcaatggt gattgtttgg aacgaccttg
     1981 ccaaaggcga ccgccaatat gctgccggat tggtagcctt taactccgtt tttcaagtgc
     2041 tactgttttc ggtttatgcc tatctcttca ttgccattct tccgggttgg tttggagtac
     2101 ccgtaaacga aactgtttct caaataacca tgggagaaat tgccaaaagt gtgttcatct
     2161 atttggggat accaatgctt gcaggtttcc ttacgcggtt catcctcaca cgggtgaaaa
     2221 gcaaaacttg gtatgaaacc aaatttgcac ctagaattag ccctttaaca ctgattgccc
     2281 tgctctttac cattgtggtg atgttctcgc tgaaaggaga atacattgta aaaatcccta
     2341 tggatgttgt gctgattgct attccgttgt tgatttattt tgtggtcatg ttctttgcct
     2401 cttttttcat gagcaaaaaa gtaggagcaa cctacgagca agcaaccacc ttatcgttta
     2461 cagcggcaag caacaatttt gaattggcca ttgccgtagc catcgccata ttcggcatca
     2521 attccggcga agcttttgca ggtgtaattg ggccactggt cgaagttcct gtaatgattg
     2581 gcttggtgaa tgtggctttt tggtttaaaa agaaatttta ttaaacctga attctatttg
     2641 atgaataagg gattagtcta gaaatcattt ttactaaata ataaataaaa cgaaaacgat
     2701 cggaaacagt tgaaataatt gtttccgatc gtttttcatt gcattcctgt tgaacagatc
     2761 aatttaaaag agctgataat aaccgaaata tagttgtcta ttatttcgaa ctggtgttat
     2821 ttaatctg
//
LOCUS       DAOPUW010000217         2827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_36857_length_2827_cov_2.771645, whole genome shotgun sequence.
ACCESSION   DAOPUW010000217 DAOPUW010000000
VERSION     DAOPUW010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36857_length_2827_cov_2.771645"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PKJ43_04635"
     CDS             complement(<1..885)
                     /locus_tag="PKJ43_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNZ71884.1"
                     /translation="MSKYVIGLDYGTDSCRALIVDASNGQEMATSVKYYPRWKEGKYC
                     DPAKNQYRQHPLDYLEVMEASIIEALSKCDPSVAKNVTGIAFDTTGSTPALTDNNGTP
                     LALLPEFAENPNAMFILWKDHTAVKEADEINELARKWEIDYTKYEGGIYSSEWVWAKV
                     LHTLRNDKTVASKAFSWVEYCDWLPGVLTGNINPATILRGRCAAGHKALWHPDWNGLP
                     PEEFLVALGKELKGFRDHLFTETHTSKEKAGTLTPEWATKLGLSTDVIVGVSAFDCHM
                     GAVGAEIEPNTLVRVIGTS"
     gene            complement(939..1622)
                     /locus_tag="PKJ43_04640"
     CDS             complement(939..1622)
                     /locus_tag="PKJ43_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HNZ71885.1"
                     /translation="MVDQLKEEVFIANIDLVKHGLVIFTWGNASGIDREKGLVVIKPS
                     GVEYDSMKPSDMVVVDLDGKVVEGKLKPSSDLMTHLVLYRNFETIGGVVHTHSTYATA
                     WAQAGRAIPNIGTTHADYFAKEIPCTRQMTENEIFSAYEYETGNVIVETFNQLNPVEV
                     PGVLVQNHGPFSWGKNAHDAVHNAVVMEQVAKMASIAFAANPALQMNPLLIRKHYERK
                     HGKNAYYGQ"
     gene            complement(1672..>2827)
                     /locus_tag="PKJ43_04645"
     CDS             complement(1672..>2827)
                     /locus_tag="PKJ43_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HNZ71886.1"
                     /translation="NMIIDQDVNASIFKDLPGLAVIFGAMWLTNIGYWGFNQYIIQKG
                     LAAKNLNEAKRGLIFAGYLKLLIPLLVVIPGITAYVLFTHPDLRGTLAGLNGSINVAD
                     DAYPWLLRNFAPNGIRGLAFAALVAAVVSSLASMLNSTSTIFTLDIYKPLFNKQASER
                     QLVKVGRISALVALVIAMVAAKPLLGGLDQAFQYIQEYTGFIYPGVVVVFGMGILWKR
                     ATNRAALWTTIVTLPIGILMKIALPELPFIIRMGYVCMILIAIAIILTLTDKNQVKAE
                     PLSEKDKKNYNLAGWVFAIISAVCMVSGVIWGTSLFDFNLLHLGFNSIFMLAFLMGFL
                     SIMMFTNANSTSKDHKAYDINPALFETDKKFLFGAMGIVIIFVALYAYFW"
BASE COUNT          765 a          587 c          594 g          881 t
ORIGIN      
        1 tgacgttccg attacccgta caagtgtgtt tggttcaatt tctgcaccta ctgcccccat
       61 gtggcaatcg aaagcgctaa ctcccacaat tacatcggta gagaggccta atttggtagc
      121 ccattctggg gttaaggttc ctgctttttc tttactagta tgtgtttcag tgaaaaggtg
      181 atcgcggaaa ccttttaatt cttttcccag tgcaaccaaa aattcttcag gtggaagtcc
      241 gttccagtcg ggatgccata aggctttgtg gccagcagca cagcgacctc tgagaatagt
      301 tgccggattt atgtttccgg tgagtactcc cggtaaccag tcacaatatt ctacccatga
      361 aaaggctttt gatgcaactg ttttgtcgtt gcgtaaggta tgtaacactt tggcccaaac
      421 ccattcagaa gagtatatac cgccttcgta tttggtataa tcgatttccc attttcgggc
      481 aagttcgttg atttcgtcag cttcttttac tgcggtatgg tctttccaaa gaataaacat
      541 cgcatttgga ttttcagcaa attcgggaag caaagctaaa ggtgtcccgt tgttgtcggt
      601 tagggcaggg gtgctaccgg ttgtgtcgaa ggctattccg gtaacatttt tagctaccga
      661 agggtcgcat tttgacaagg cttcaataat tgaagcttcc attacttcca gataatcaag
      721 cggatgttga cgatattggt tttttgccgg atcgcaatac tttccttctt tccagcgtgg
      781 gtaatatttt acactggttg ccatctcctg tccgttcgat gcgtcgacaa ttagtgccct
      841 gcatgaatcg gttccataat ctaatcctat aacgtattta ctcatcgttg tttatttttt
      901 tgatattgac gctatttatg cggttcaaca tttatttttt attggccata ataggcgttc
      961 tttccatgtt ttctttcgta gtgttttctt atcagcaaag ggttcatttg caatgctgga
     1021 tttgcagcaa atgcaataga agccattttt gctacctgct ccatgactac agcgttgtgt
     1081 acagcatcat gggcgttttt tccccaggaa aatggtccgt ggttttgtac cagaacccca
     1141 ggaacttcta ccgggtttag ctggttgaat gtttcaacta taacgttccc ggtttcgtat
     1201 tcgtaggctg aaaaaatttc attttcagtc atttgtcgtg tgcaaggtat ttccttggcg
     1261 aaataatcgg catgcgttgt gccaatgttg ggtatagccc ttccggcttg agcccatgct
     1321 gttgcgtagg ttgaatgagt gtgaacaaca ccgccaattg tctcaaaatt tcggtaaagc
     1381 accaaatgtg tcatgagatc tgacgagggt ttaagtttcc cttccacgac tttcccgtcc
     1441 aaatcaacaa ctaccatgtc gcttggtttc attgaatcgt attcgacacc gcttggcttt
     1501 attactacca agcctttttc acggtcaatt ccgctggcat tgccccaggt gaaaattacc
     1561 aagccatgtt ttaccaagtc gatattggca ataaaaactt cttcttttag ttgatctacc
     1621 atattaaatt aataatatgg gcaccctaat atagggtgcc caagtatttg tttaccagaa
     1681 atatgcataa agtgcaacaa agataattac aatacccata gctccgaaaa ggaatttttt
     1741 atcggtttcg aaaagtgcag ggttaatgtc gtaagctttg tggtcttttg aagttgaatt
     1801 tgcgttagtg aacatcatga tcgagaggaa tcccatcagg aaggctaaca tgaaaataga
     1861 gttgaatcca agatgtaaca ggttgaaatc gaagaggctg gttccccaga taactccaga
     1921 taccatacaa actgcagaaa taatagcaaa aacccaaccg gcaaggttgt aatttttctt
     1981 gtctttttct gacagaggct ctgctttcac ctggttcttg tctgtcaatg tgagtataat
     2041 tgcaatagca atcaggatca tacatacata acccattcga atgatgaatg gtaattcggg
     2101 taaggcaatc ttcattaaga taccaattgg gagagtaact atggttgtcc aaagtgctgc
     2161 acggttagtt gcacgtttcc aaagtatacc cataccgaat actacaacta cacctgggta
     2221 gataaaccca gtgtattctt gtatgtattg aaatgcttgg tctagtccac ccaataatgg
     2281 ttttgcagct accatggcaa ttaccagggc aactaatgct gaaatcctac ctactttaac
     2341 aagttgtctt tccgatgctt gtttgttgaa aagtggtttg tatatgtcaa gtgtgaaaat
     2401 ggttgatgtt gaattcagca tcgaagcaag cgatgataca acggcagcaa cgagagcagc
     2461 aaaagcaagc ccgcgaatcc cgtttggtgc aaaattacga agtaaccatg ggtaagcatc
     2521 gtcggcaaca ttaatactgc cgttcaatcc tgctaatgtt ccccttaaat cgggatgtgt
     2581 gaacaatacg tatgctgtga tacctggaat tacaaccaaa agtgggataa ggagtttcaa
     2641 atatccggca aaaataagtc cacgctttgc ctcattgagg tttttggcag ccaacccttt
     2701 ttggataata tattgattaa agccccaata gccgatattt gtgagccaca tagctccaaa
     2761 gataactgct aatcccggaa ggtctttaaa aattgaagca tttacatctt ggtcaataat
     2821 catgtta
//
LOCUS       DAOPUW010000218         2799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_37352_length_2799_cov_2.483965, whole genome shotgun sequence.
ACCESSION   DAOPUW010000218 DAOPUW010000000
VERSION     DAOPUW010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37352_length_2799_cov_2.483965"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PKJ43_04650"
     CDS             <1..430
                     /locus_tag="PKJ43_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNZ71887.1"
                     /translation="VFDKAMSELKITEDDFKQLTAFVKEMKFDRLGVFPYSHEEDTYA
                     FTQYPDDIPQEIKQERADEIMEIQQSISESLMQQKVGQTINVLIDREEDEYYVGRTEW
                     DSPEVDGEVLIPVSAQLEIGQFYKVKINSAFMFDLYGDVI"
     gene            437..1792
                     /locus_tag="PKJ43_04655"
     CDS             437..1792
                     /locus_tag="PKJ43_04655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNZ71888.1"
                     /translation="MLKGYFPFYKRLLRIAFPLVLTQAGQMSVQLIDNAMVGRVGTAE
                     LAAASFANSIYIIIMVFGLGILLGLTPLISHSLGGGNEKKIAFHIKNGFILTLIVSGI
                     LTLVTLSLTAFMPYMGQSEDVVRLAIPYFKTLAISLIPLLLFIFLKQTGEGFGNTILA
                     MYATLLANLINIVLNYLLIYGKFGFPELGLLGAGYATLISRVVAPIFLFVCFRKVSPI
                     NHFIAISQKIKASKEEILRIFRLGLPIAGQLVLEVSAFAIGAVMMGWLGSVPLAAHQV
                     AMGMASFTFMLANGVAQASTIRVSYQLGQKDFISMEKVSLSAVHLVVVYMGICGVLFF
                     TLRNILPMIFTTDPVVIKQAASLLMVAALFQLFDGLQVVSMGILRGFKDVNAPMMIAG
                     ISYLIIGLPVSYVFAFILGFGPEGIWFGFVGGLISAAILLSFRIRKNIREVEANNIQT
                     N"
     gene            complement(1809..>2799)
                     /locus_tag="PKJ43_04660"
     CDS             complement(1809..>2799)
                     /locus_tag="PKJ43_04660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNZ71889.1"
                     /translation="EKDIEILKQQGLVESLGKPAKIWLGVPLIVEGKALGVFTLQSYD
                     DEKAFSLKDKEMIEFVTHEISHTVRRIKAEEEIILALERAEQSDRLKSAFLANISHEI
                     RTPLNSILGFTKLLGEPDVDAIKLHRYAEIILNNGNQLLSIINGVLDFSMIETGQIKL
                     LKKRFAIEKVVQKVYSDFLPAATQKQLELIISPDLFVNETFIDADYSRLKQAVANVVD
                     NAIKFTKKGTVELSMNRGENFVQIVVRDTGIGIPYDFKKSVFDQFQQADRSSVRRYGG
                     NGLGLAIAKYMIELMGGQIWFESELNAGSVFYIEVPYKHSASDTFFTGAKSKK"
BASE COUNT          767 a          580 c          585 g          867 t
ORIGIN      
        1 agtttttgac aaagcaatgt ctgagcttaa aataactgaa gatgatttca agcaactaac
       61 agcctttgta aaagaaatga aattcgaccg gttgggtgtt ttcccgtatt cgcacgaaga
      121 agacacctac gcctttaccc aataccccga tgatattcca caggagatca agcaggaacg
      181 cgccgacgaa atcatggaaa tacagcagtc gatttcggaa tcgcttatgc aacaaaaagt
      241 ggggcaaacc atcaacgtac tcatcgacag agaagaagat gaatattatg ttggtcgcac
      301 tgaatgggat tcacctgaag tagacggtga agtgttgatt cccgtttcgg cacaactcga
      361 aattggccaa ttctacaagg tgaagattaa cagtgcgttt atgttcgatt tgtatggtga
      421 tgtgatttaa cagcatatgc tgaaaggata ctttccattt tataagcggc tactcagaat
      481 tgcttttccg ctggttctaa cccaagcggg acaaatgagc gtgcagctga ttgataatgc
      541 aatggttggt cgtgtaggta ctgcagaact tgcagcagcg agttttgcga acagtatcta
      601 tataatcatt atggtttttg gcctcggaat actgctcggg ttaacaccat taatcagcca
      661 ttcgttaggt ggtggaaacg aaaaaaaaat agctttccat ataaaaaatg gctttatctt
      721 aacccttatt gtttcgggta tactaaccct cgtaaccctg tctcttactg cctttatgcc
      781 ctacatgggt caaagcgaag atgttgtcag gttggctatt ccttatttta aaaccctggc
      841 catatcatta atccctttat tactgttcat ttttctgaaa caaaccggtg aaggttttgg
      901 caatacgatt ttggccatgt atgccacgtt attagccaac ttgataaata ttgtcctgaa
      961 ttacctgctc atttacggaa agttcggttt ccccgaactg ggattgcttg gggctgggta
     1021 tgctacgctt atttcaaggg ttgttgctcc aatttttctg ttcgtctgtt tcaggaaagt
     1081 atccccaata aaccacttta ttgctataag tcaaaaaatt aaggccagca aagaggaaat
     1141 tttacggatc ttcaggttgg gattaccaat tgccggccaa ttggtgctcg aagtctcagc
     1201 tttcgcaatt ggagcagtta tgatgggatg gcttggcagc gtacccctgg cagcccacca
     1261 agtggccatg ggcatggcaa gtttcacttt catgctggcc aatggtgttg cccaggcatc
     1321 aaccattagg gtaagctatc aacttggaca aaaagatttt atcagcatgg agaaagtttc
     1381 gctctctgct gttcatctgg tcgttgtgta tatgggaatt tgcggggtct tgttctttac
     1441 cctgcggaat attttgccaa tgattttcac gactgaccct gtagtcataa agcaagcggc
     1501 atcgctgctt atggtcgctg ctttattcca attattcgac gggcttcagg tagtttcaat
     1561 gggaatattg cgtggtttta aagatgtaaa cgctccaatg atgattgccg ggatatctta
     1621 tttaattatc gggctacctg ttagttatgt ttttgctttt atccttggtt ttggccctga
     1681 aggaatctgg ttcggttttg ttggtgggct catcagcgcg gccatcttgt tatcgttccg
     1741 tatacgaaag aatattcgag aggtggaagc aaacaacatt caaacaaatt aaaccgacaa
     1801 aatgaaagtt atttctttga ttttgcccct gtaaagaacg tatcagatgc tgagtgttta
     1861 taaggaactt cgatataaaa tacagaacct gcgttaagct ccgattcgaa ccaaatttgc
     1921 ccccccatga gttcaatcat atatttggca atagctagtc ccaatccatt tcctccgtat
     1981 cgtcttacac tgctacgatc agcttgctgg aattgatcga aaacgctttt tttgaaatcg
     2041 taaggtattc caattcctgt gtcacgaaca acaatttgga caaaattttc gccacggttc
     2101 atgctcaatt caacagtccc ttttttagta aattttatgg cattgtcaac aacgttagca
     2161 acagcttgtt ttaacctgga gtaatctgca tcgataaaag tttcatttac aaacaaatct
     2221 ggagaaataa ttaattcaag ttgcttttgt gttgctgcag gcaggaagtc ggaatatact
     2281 ttttgcacta ctttttcgat ggcgaaacgc ttcttcaaca gcttaatctg gccggtttcg
     2341 atcatcgaaa agtcgagaac cccattgatg atcgacaaaa gttgattccc gttattgagt
     2401 attatttcgg cataacggtg caatttaatg gcatcaacat ctggctctcc aaggagcttg
     2461 gtaaagccaa ggatactatt caaaggggtt cgtatttcgt gactaatgtt ggcaagaaaa
     2521 gcagacttta acctatcgct ttgttctgca cgctcaagcg ccagaattat ttcttcttcc
     2581 gctttaatgc gccttacggt atgactgatt tcgtgggtga caaattcaat catttcctta
     2641 tctttcaagc taaaagcctt ttcatcatca tagctttgca aggtaaaaac cccaagcgct
     2701 ttcccttcaa caataagagg aacccctaac caaattttgg cgggcttacc caacgactcg
     2761 acaagccctt gctgtttcaa tatttcaata tctttttcg
//
LOCUS       DAOPUW010000219         2797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_37376_length_2797_cov_3.896061, whole genome shotgun sequence.
ACCESSION   DAOPUW010000219 DAOPUW010000000
VERSION     DAOPUW010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37376_length_2797_cov_3.896061"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1195)
                     /locus_tag="PKJ43_04665"
     CDS             complement(5..1195)
                     /locus_tag="PKJ43_04665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNZ71890.1"
                     /translation="MNNTYTKIVLKSGKDQSLKRFHPWVFSGAVKKIYGTVSEGDIVS
                     VYSNQDEFLGLGHYQIGSIAVRVFSFTEVNPDRDFWKSKLEEAYRLRAALGLTNSPQT
                     NVYRLVHAEGDGMPGLIIDIYNGTAVMQMHSIGMFQIRMLLAEILKEIMGDKLKAVFD
                     KSSKTIPFKAGIEATDGFILGESSPEMVTENGLHFNVDWVEGQKTGFFIDQRDNRQLL
                     SQYSEDRDVLNMFCYTGGFSFYAMKGGARSVHSVDSSGKAIDLTRENVELNYPNDKRH
                     EAIVADAFDFLKDIKDKYDLIVLDPPAFAKHREALGHALQGYKRINAKAFEQIRPGGI
                     LFTFSCSQVVSKEKFREAVFSGAAIAGRTVRILHQMTQPADHPVSIYHPEGEYLKGLV
                     LYVE"
     gene            complement(1188..1772)
                     /locus_tag="PKJ43_04670"
     CDS             complement(1188..1772)
                     /locus_tag="PKJ43_04670"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNZ71891.1"
                     /translation="MIYKSSISKEEFDELPFYSFDGEIVVVDTKEKMREAANYMAQFP
                     YLGFDTETRPSFKKGQINQVALLQLSTHDRAFLFRLNKQEISRKIIEILADEKIIKAG
                     VAIRDDIKVLQSKQKFSPAGFVELQDEARKRGIESFSLKKLAAIVLGLRISKAQQLSN
                     WEADELTEPQLRYAATDAWLSLKVYEHFKNHENE"
     gene            complement(1762..2400)
                     /locus_tag="PKJ43_04675"
     CDS             complement(1762..2400)
                     /locus_tag="PKJ43_04675"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HNZ71892.1"
                     /translation="MKEQNSEHIAFSKAAIEFAAVANEYCNFIEGQQQYPLKAFLSTL
                     QKLLPLLYLKTSMLPPIDDENAETPEKYLSEVDYNFLLGKLNNKLGQHDTFLEVFDQA
                     MQFSETSVEQSISENLCDIYQDLKDFIFVYRMGTTELMNDALWECKNNFEEFWGQKLV
                     NVLRAIHSLVYSDALNEEQTTDPGQKNKKGNNWVSKHFRNQESNEEFSDNDL"
BASE COUNT          713 a          592 c          638 g          854 t
ORIGIN      
        1 ctggttactc aacatacagg actaaacctt taagatattc tccttctgga tgataaatgc
       61 ttaccgggtg gtcggctggc tgggtcattt gatgtagtat cctgacggtc cgtccggcaa
      121 ttgccgcgcc tgaaaagaca gcttccctaa atttttcctt gcttaccacc tgcgaacagc
      181 taaaagtgaa caagatgcct cctggtctga tctgttcaaa cgcttttgcg ttgattcttt
      241 tatacccttg cagcgcatga ccaagggctt cgcggtgttt ggcaaaagca ggaggatcga
      301 gcactatcag gtcgtatttg tcttttatat ctttcagaaa atcaaatgca tcggcaacaa
      361 tggcttcatg gcgcttgtca ttcgggtaat taagttcgac gttttcgcgg gtaaggtcaa
      421 tggctttccc cgatgaatcg actgaatgca cgctgcgggc accccctttc atggcgtaaa
      481 acgagaaacc accggtgtag cagaacatgt tcagtacatc gcggtcttcc gagtattggg
      541 aaaggagctg acggttgtcg cgctgatcga tgaaaaaacc agttttctgg ccttcaaccc
      601 aatcgacatt gaaatgtagc ccgttttcgg ttaccatttc aggcgagctt tctccaagga
      661 taaagccatc agttgcttca attccggctt taaacgggat ggttttcgag cttttatcga
      721 aaacagcctt gagcttatct cccataattt ctttcaatat ttcagcaagt agcatgcgta
      781 tctggaacat tccaatggaa tgcatttgca ttaccgcagt gccgttgtat atgtcgatga
      841 tcagcccggg cataccatcc ccttcggcat gaaccagacg gtaaacattg gtttgcgggc
      901 tgttggtaag gccaagggct gcgcgtagcc tgtaggcttc ttcgagtttc gatttccaga
      961 aatcccggtc ggggtttacc tcggtaaacg aaaaaacacg aaccgcgatg gagccgatct
     1021 ggtaatggcc taagccaaga aattcgtcct gattggaata aaccgaaacg atgtcgcctt
     1081 cgcttactgt tccgtatatt tttttcacgg cgcccgaaaa aacccagggg tgaaaccgtt
     1141 ttagcgattg atctttcccc gatttaagta ctattttagt gtatgtgtta ttcattttca
     1201 tggtttttaa aatgctcata aacttttaac gaaagccagg cgtcggttgc agcatagcgt
     1261 agctgcggct cggtaagctc gtcggcttcc cagttcgaca attgctgggc ttttgatatg
     1321 cggagtccaa gtacaatggc tgccagtttt tttaggctga aagactcgat tccccttttc
     1381 cgggcttcgt cctgcagttc aacaaatccg gcaggtgaaa atttttgttt cgattgcagt
     1441 actttgatgt cgtcgcgaat ggcaaccccg gcttttatta tcttttcgtc agcaagtatt
     1501 tctatgattt tgcgggaaat ttcctgtttg ttcaacctga aaagaaaagc ccggtcatgg
     1561 gtcgatagct ggagtaaagc cacttggttg atttgccctt ttttaaacga aggccgtgtt
     1621 tcggtatcga atcccagata cggaaactgt gccatataat tagcggcctc acgcatcttt
     1681 tctttggtgt cgacaactac aatttcacca tcgaaggagt aaaacggaag ttcgtcgaat
     1741 tcttcttttg aaatgcttga tttataaatc attgtcggaa aactcttcgt tactttcttg
     1801 gttcctgaaa tgttttgaaa cccagttatt gccttttttg tttttttgtc cggggtcggt
     1861 agtttgctct tcgtttaggg catcgctgta tactaacgag tgaatggccc gaagtacgtt
     1921 gaccagtttt tgcccccaaa attcttcaaa gttgtttttg cattcccata aggcatcgtt
     1981 catcaattca gttgtcccca tacggtacac gaaaatgaag tctttcaaat cctggtagat
     2041 atcgcaaagg ttttcagaaa tgctctgttc tacagaagtt tcgctgaatt gcatggcctg
     2101 atcgaagact tcgagaaagg tgtcgtgctg cccaagttta ttgtttagtt tcccaagcag
     2161 gaaattgtaa tcgacctcgc tgaggtattt ctcaggggtt tctgcatttt cgtcgtcgat
     2221 aggcggcaac atgctggttt taaggtagag caggggaagt aacttttgca gagtcgacaa
     2281 aaaagccttc aacggatatt gctgctggcc ttcaataaaa ttacaatact cgttggctac
     2341 agccgcgaat tcgattgctg cttttgaaaa agcgatatgt tctgaatttt gttctttcat
     2401 acgtattgag tttgcaaaaa taagtaaata gatagatttt gtcctaaaat tttaccaata
     2461 ttgaaaagcc gggcgtgctt gactcatttt ttgagtgact ggccgaagtg cagcatcagg
     2521 ataagggaga agaaacaacg cctgatgcga tgataaaaaa taagatcgct ttgcatgcat
     2581 ggttcatcag gataaaagta ttttgagagc ctgtatttta ttgataatgt tcgaaatcag
     2641 ataacaaaaa acgatgtgcc tgtgaaagtt ttaacgatgt ataccacaac cttttcattt
     2701 gtgccggcgc tgaagaatag cgattttgcc gatgaaaatg ccgaagcaaa agagttttct
     2761 gactgcattc tggcctttat tcagattgaa gcagaag
//
LOCUS       DAOPUW010000220         2792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_37467_length_2792_cov_3.775667, whole genome shotgun sequence.
ACCESSION   DAOPUW010000220 DAOPUW010000000
VERSION     DAOPUW010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37467_length_2792_cov_3.775667"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="PKJ43_04680"
     CDS             complement(<1..966)
                     /locus_tag="PKJ43_04680"
                     /inference="COORDINATES: protein motif:HMM:NF016593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNZ71893.1"
                     /translation="MNTININTKITKLSGSIADLVTPVSVYLKMRDRYPNTLLLESSD
                     YHGSSNSMSYICFDEIATIEVNKGIINQCFPGQEPRKEAVSASNNVADNLQAFLKTFN
                     TPEIKQKGLVNGLWGYTAFDAIQYFETIKLRERENNEAEIPEIRYAFHRYVLAINHFN
                     NDISLIENLLEGEESHAAELTGILLNQNVVTFQFKTEGEETSNITDEEYMQMVDEGKK
                     HCFLGDVFQIVLSRRFRQKFSGDEFNVYRALRRINPSPYLFYFDFCNYKIFGSSPEAQ
                     LIVSKNKAIINPIAGTFKRTGDDEKDRQLAIDLSADKKENAEHVML"
     gene            complement(1240..>2792)
                     /locus_tag="PKJ43_04685"
     CDS             complement(1240..>2792)
                     /locus_tag="PKJ43_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNZ71894.1"
                     /translation="EDIKPLMDNTTEMGNVARMISDYHQKNEEIEASESTKRHIIEQV
                     QVGIIISDAASSLIITANPYACEIINAPEDAILGNITQNFFAPLSDELKNKLKNTTEN
                     LTGIESFIQSSTGEQIPILRNVTRMFMDGKHAIMETFINLSEIKSLQNKLEDEKKKHS
                     LAMKNSGLVFCEYDFKTDEILLSDEWKFMLKGKSNSNAQNIINNIYPSDYKSITDHFS
                     SLSSGARDTLTSEFRVQHPERGIIWVNLSVLITKRDEAHKPKQLIGLLEDITERISIQ
                     QELIKAKERAEESDRQKSAYLGNMSHKIRTPLNTIVGFSNLLTEDDISHQEKDNYINI
                     IRHDTEQVLHLIDDIINIAKIDANQLDTNDKECSINEMFNSLSEYYKTNEKTNRIEFV
                     TKQMLPNGKDILKTDADKLHQILNNLLNNAFKFTNEGKIELGYFINPVEEKMIIYVKD
                     TGIGIQNEHKDKVFNHFYQANQTTEGTGLGLTISQSLVKLIGGKLNFESKEGEGSTFF
                     VELPFKEV"
BASE COUNT          680 a          519 c          571 g         1022 t
ORIGIN      
        1 caacattacg tgttctgcgt tttctttctt gtcggccgaa aggtcaatgg ccaactgacg
       61 gtctttttcg tcgtcgccgg tacgtttgaa tgttcctgca atagggttga tgatcgcttt
      121 gtttttgctc acaatcaatt gtgcttcagg cgatgaacca aatattttgt aattgcagaa
      181 gtcgaaatag aaaaggtaag gcgaggggtt aatacggcga agggcacggt aaacattgaa
      241 ctcatcgccc gagaattttt ggcggaagcg gcgcgaaagc acaatctgga acacatcgcc
      301 caagaagcag tgtttcttgc cttcgtcaac catttgcatg tattcttcgt cggtgatgtt
      361 cgatgtttct tcgccttcgg ttttgaactg gaaggttacc acattctgat tcaataggat
      421 acctgttaat tcagcagcgt ggctttcttc tccttcgagc aggttttcga tcagcgaaat
      481 gtcattgtta aaatgattga ttgctagcac ataacggtga aaagcataac gaatttcagg
      541 aatttcggct tcgttgtttt cgcgttctcg tagttttatg gtttcgaaat attggatggc
      601 atcgaatgcc gtatagcccc acaaaccatt aaccaaccct ttttgcttga tttcgggcgt
      661 attgaacgtt ttaaggaaag cttgcagatt gtcggccaca ttgtttgaag ccgaaactgc
      721 ttcctttcga ggttcttgcc caggaaagca ttggttaata atgcctttgt ttacttcgat
      781 tgttgcaatt tcgtcgaaac aaatgtagct catgctgttg ctcgatccgt gatagtcgct
      841 actttctaac aacagggtgt tcgggtagcg gtcgcgcatt ttcaggtaca cgctcactgg
      901 cgtaaccaag tcggcaatgc ttcccgatag cttggtgatt ttggtgttaa tgttgattgt
      961 gttcatatcg taatttttta ttgtttctga ttttttgaaa tgaaaaaagg cttgccggaa
     1021 gttccgacaa gccttaataa tatcttttca ctatattaca tgagtagcct gctcaacttc
     1081 ctattgagaa ttttaagctg tgccaccacc atgtatttac agttcttgtt gtcatttttg
     1141 ctttgtttac gtttgcaaat aaaaaaattg attttcaata ttcctaatat ttcaacttaa
     1201 tttttttgat ttttattgaa cgttaatttt agtcgttgat tagacctctt tgaaaggcaa
     1261 ttcgacaaag aacgttgaac cttcgccttc ttttgattca aaattgagtt ttccaccaat
     1321 gagcttcact aaactttggc tgattgttaa gcctaatcct gtgccttcag ttgtttggtt
     1381 ggcttgataa aaatggttga aaactttgtc tttatgctcg ttttgtattc caattccagt
     1441 atctttcaca taaatgatca tcttttcttc aaccggattt atgaagtagc ctaattcgat
     1501 tttaccttcg tttgtaaatt tgaaagcgtt atttaacagg ttattcagaa tttgatgaag
     1561 cttatcagca tcagttttta aaatgtcttt tccattaggc agcatctgtt ttgtaacgaa
     1621 ctcgattcgg ttcgtttttt catttgtttt atagtattct gacagcgaat tgaacatttc
     1681 gtttatgctg cactctttgt cgtttgtgtc gagttgattc gcgtcaattt ttgcgatgtt
     1741 gataatgtca tcaatcaggt ggagcacctg ttctgtgtcg tgcctgataa tattgatgta
     1801 attatctttt tcctgatgag agatatcgtc ttcggttaag agatttgaga acccaacaat
     1861 agtatttaac ggagtccgaa ttttgtggct catatttcca agataggccg atttttgcct
     1921 gtcagattct tcggcccttt ctttagcttt aatcagttct tgttgaatac taatgcgttc
     1981 tgtaatatct tcgagcaagc cgattagttg ttttggcttg tgtgcttcgt cacgttttgt
     2041 aatcagaacg gataaattaa cccaaataat tcccctttcc gggtgttgaa ccctgaattc
     2101 ggaggtcaga gtatcgcgag ctcctgaaga cagggagctg aaatgatcgg taattgattt
     2161 atagtctgat ggataaatgt tgttgatgat gttttgtgca ttgctgtttg attttccttt
     2221 aagcatgaat ttccattcgt cggacaacaa aatttcatcg gttttgaagt catattcaca
     2281 gaaaacaagc cctgaatttt tcattgccaa actatgtttc tttttctcgt cttcaagttt
     2341 attttgcaac gatttgattt cgctcaggtt aatgaaagtt tccataatag catgtttgcc
     2401 atccatgaac attctggtaa cgtttcttaa aattggtatt tgttcaccgg ttgagctttg
     2461 tatgaagctt tctataccgg ttaaattctc ggtagtattt ttgagtttgt ttttcagttc
     2521 atcgctgagt ggggcaaaga aattttgtgt aatgttcccg agaatagcat cttccggagc
     2581 attaattatt tcgcatgcat aagggtttgc agtaatgatc aggctcgaag ctgcatctga
     2641 gatgattatt ccaacctgaa cttgttcaat tatatgccgt ttagtgcttt cgctggcttc
     2701 aatttcttca tttttctgat ggtaatcgct aatcatgcgg gccacattgc ccatctcggt
     2761 agtattatcc atgagtggct taatatcctc tg
//
LOCUS       DAOPUW010000221         2792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_37474_length_2792_cov_3.313482, whole genome shotgun sequence.
ACCESSION   DAOPUW010000221 DAOPUW010000000
VERSION     DAOPUW010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37474_length_2792_cov_3.313482"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /gene="nfo"
                     /locus_tag="PKJ43_04690"
     CDS             <1..608
                     /gene="nfo"
                     /locus_tag="PKJ43_04690"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010424716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HNZ71895.1"
                     /translation="PLQKSREAFLDEMHRCEQLGLDRLNFHPGSHLGKVDVDVCLKTI
                     ADSINWALDRTSGVIAVIENTAGQGSNVGNSFEEIRKTIDHVEDKSRVGVCIDTCHAF
                     TAGYELNTLEGYSLTWKKFEEIIGFEYLKGMHINDSKKELGTRVDRHDSLGKGFIGNN
                     LFEWIMNDPRMDGIPLILETPNEEIWAEEIALLKSYSKNSR"
     gene            739..1938
                     /locus_tag="PKJ43_04695"
     CDS             739..1938
                     /locus_tag="PKJ43_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ71896.1"
                     /translation="MSIDRMFTWSFQQIQRTNTLFKRYLYNQLDWNARLSIIVGMRGV
                     GKTTMMLQHAREHLPFDNSVIYISIDDIYFTTNSLIDFAEEFNKRGGKYLLIDEVQKY
                     EGWSREVKMIYDNFPEIQIIVSGSSAIEILKGEGDLSRRAIINRLQGLSFREFIELKY
                     QQPFPVWTLSEILKNPVEKAFLITHKIKPIKLFEEYIQAGYYPFAVEDERSFQQRMMQ
                     IINTIIETDIPAVNSIDYNAVLKMKKLLGIIAESVPFKPNISKLANQLSLSRETFLKY
                     LQLLAKADIISLMYSSVKGVSALNKPEKIYLNNPNIAYLLADSNANTGNMRETFFMNQ
                     LQHIHNVEYPAKGDFLVDETYTFEVGGKNKTTQQFAGIENAYVVADNIEIPVGNKIPL
                     WMFGFLY"
     gene            2287..>2792
                     /locus_tag="PKJ43_04700"
     CDS             2287..>2792
                     /locus_tag="PKJ43_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71897.1"
                     /translation="MVSKHSIAVIPFQNLSTDIDCEYFSDGITEEIINALSKIEGLNV
                     TSRTSSFKFKNQPFDSCTIGEKLNVSFLLEGSVRYSGNNVRISAKLINAKSGFQIWAE
                     NWDRQLKDIFIIQDEIAETIALKINKQIIPASQNTEVAVEDTFAIDMYLRGQYLLNQL
                     DSSKSGQM"
BASE COUNT          912 a          492 c          583 g          805 t
ORIGIN      
        1 aaccattgca aaaaagccgc gaagcttttc tcgatgagat gcatcgttgc gaacagcttg
       61 gtctcgatcg gttaaatttt catccgggaa gtcatctggg taaggttgat gttgatgtct
      121 gcctgaaaac aattgccgat agcataaact gggcacttga ccgcaccagc ggggtaattg
      181 cggtgattga aaacactgca ggacaaggct caaatgttgg gaacagcttt gaagaaatcc
      241 gtaaaacaat cgaccatgtt gaagacaaaa gccgtgttgg ggtttgtatc gatacctgcc
      301 atgcatttac tgctggctac gagttgaata cattggaagg ttacagtctt acctggaaaa
      361 agtttgaaga gattattggc ttcgaatatc tgaaagggat gcacattaac gactcgaaaa
      421 aggaacttgg aacccgagtt gaccgccacg atagtctggg aaaaggcttt attggcaaca
      481 acctttttga atggataatg aacgacccac ggatggatgg tattcctctc attttggaaa
      541 ctcctaacga agagatttgg gccgaagaaa ttgcacttct gaagagttat tcgaaaaatt
      601 cacgctaatt ctcagattgt tgtaatgtag agtctagtat tgaatgtgta aaatgtagaa
      661 tgggaattac attttagttt aaaaatgtaa aattaattca acatttttgt ttatttttgt
      721 tggatcaatt ttgcaattat gtcgatagat agaatgttta catggtcatt ccagcagatt
      781 caacgaacaa ataccctgtt caaacgctat ctgtataatc aacttgattg gaatgcaaga
      841 ttgagtatta ttgttggaat gcgaggtgtt ggaaaaacaa ccatgatgct acaacatgcc
      901 cgcgaacatc ttcctttcga caattcggtg atttatattt cgatcgatga tatttacttt
      961 actacgaatt ccctcattga ttttgctgaa gaatttaaca agcgaggcgg taaatatctt
     1021 ctaattgatg aagtgcaaaa atatgaagga tggtcgaggg aagtgaaaat gatttacgac
     1081 aactttcctg aaattcaaat cattgtaagt ggaagttctg caattgagat actgaagggt
     1141 gaaggcgatt tgagccgaag ggcaattatt aaccggttac aaggattgtc ttttcgcgaa
     1201 ttcatagaac taaaatatca gcagccattt ccggtttgga cacttagcga aatactcaaa
     1261 aaccctgttg aaaaggcatt tttaattacc cacaaaataa aaccgataaa gctgttcgaa
     1321 gagtatattc aggcagggta ctatccgttt gccgttgaag atgagaggag ttttcagcaa
     1381 cgaatgatgc agataatcaa cacaattata gaaactgaca ttcctgctgt aaactccata
     1441 gattacaatg ctgtactcaa aatgaaaaaa ttactgggaa ttattgccga gtcggttccg
     1501 tttaaaccca acatttcaaa attggccaat caattatcgc tttcgcgcga aacgttccta
     1561 aagtacctgc aactgcttgc aaaggctgac attattagct tgatgtactc ttcggtgaag
     1621 ggggtaagtg cgcttaacaa gcccgagaaa atttacctga acaacccgaa tattgcatat
     1681 ttattggcag attctaatgc aaacactggg aatatgcggg agactttttt tatgaatcaa
     1741 ctgcaacaca tccacaacgt agaataccct gcgaaaggcg attttctggt tgacgaaaca
     1801 tacacctttg aagttggagg taaaaacaaa accacgcaac aatttgcagg aatcgagaat
     1861 gcttatgtgg tagctgacaa tattgagata cctgttggca ataaaatccc gctgtggatg
     1921 tttggatttt tgtattaacc aaacagttag gcataagtta ttggaaatat tgcctcgatc
     1981 gaatttagca cgtttccctg aaatcgttac gaagcactct gaattcggta cggcggtcga
     2041 taattgacaa cactttgctc ccgtttttgc gaaagctagg agttgaactg aaccgatcca
     2101 acaaagtcgg tgcgagtcat aatctcgata acaatggttg ggtttagttc aagaagtaaa
     2161 attttactta tcggaaaatg tcatcttctg gaaaaagcat ctgatgactt tttctcaaga
     2221 gcccgctatt ctattttcac tttctgataa attagtaaat tgcatttttt taatccaaaa
     2281 agtaaaatgg tttcgaaaca cagcattgcc gtaatccctt ttcaaaatct gagcactgat
     2341 atagactgcg aatatttcag cgatgggatt actgaagaaa ttatcaatgc cctttcaaaa
     2401 atagaaggac ttaatgttac atcgcgtact tcatcgttta agttcaaaaa ccaacctttt
     2461 gatagttgta cgatagggga aaaattaaat gtatccttcc tgcttgaagg aagtgtcaga
     2521 tattcaggaa ataatgtaag gatttcggca aaactgatca atgcaaaaag tgggtttcaa
     2581 atttgggccg aaaactggga cagacaatta aaagatattt tcatcattca agatgaaata
     2641 gctgaaacta ttgcactgaa aataaacaag caaataatac cggcctcgca aaataccgaa
     2701 gttgcggttg aggatacctt tgccattgat atgtacctaa ggggtcaata tcttttaaac
     2761 cagctcgata gtagtaaaag cgggcaaatg tt
//
LOCUS       DAOPUW010000222         2783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_37621_length_2783_cov_3.155059, whole genome shotgun sequence.
ACCESSION   DAOPUW010000222 DAOPUW010000000
VERSION     DAOPUW010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37621_length_2783_cov_3.155059"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PKJ43_04705"
     CDS             complement(<1..723)
                     /locus_tag="PKJ43_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNZ71898.1"
                     /translation="MKKFQKVVFTVLIAITGGIVGMFVYTQFFEKPNVVTVTQPTAVQ
                     YASYNPQGSPDLTYAAEFSVKAVVNIQTSYKKDQSYSSGNPFFDFFFGDRYYYEQPQV
                     QQATGSGVIISEDGYVVTNNHVIESSNEIKVTLHDNREYEAKLIGTDPSTDIALLKIE
                     ASGLPTLSWGDSDNLKLGEWVLAVGNPFNLNSTVTAGIVSAKARGIGIISGQMPIESF
                     IQTDAAVNPGNSGGALVNTKGEL"
     gene            894..1673
                     /gene="dapF"
                     /locus_tag="PKJ43_04710"
     CDS             894..1673
                     /gene="dapF"
                     /locus_tag="PKJ43_04710"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNZ71899.1"
                     /translation="MKLEFYKYQGAGNDFIMIDNRAGFFDHDNQALIESLCHRRFGVG
                     ADGLILLENASGYDFKMVYYNSDGREASMCGNGGRCIVAFARQLGIISTSTLFVASDG
                     PHEAFVDDMGLVDLKMIDVEKIESLVDGYFLNTGVPHLVHFVDDLHTTDVELEGRQLR
                     WDARFQPAGTNVNFVKIEGDHLTVFTYERGVEAETLACGTGITASALSAAFKTGKISG
                     EYKITAKGGDLTVRFQQDSGRYTNIWLKGPALLVYAGSVNI"
     gene            1723..>2783
                     /locus_tag="PKJ43_04715"
     CDS             1723..>2783
                     /locus_tag="PKJ43_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71900.1"
                     /translation="MSGDWFRKLPLRGSIIAFLIIATFLIYFKTLSFGFLNFDDGQFV
                     TENEVVSSDTSSFADCFRYKFGEHDYFPLTYAFFRLLRLLFGFNPVVFHATNLLFHIA
                     NVVLVFLLCMKILTQFWPGRKNNELSAAIIAFIFSFHPIHVESVSWVIDLKDMLFTFF
                     YLSALLLYWKWIDSRKIVFYYLAFFLAFLSLLSKSTAITFVAALFWVDFAIGRKGLKE
                     MIIEKIPFFLLTLTGFYIFGLFTNPGGALLGLTGGMAEGAAPYFPASVASLPFFLQRI
                     VIASFRLGFWIMHSVFPFELNLFLMRSVLLEKYALLLPFIPFLLVAGFSGIWLLRKKT
                     PLMLYGLAFRMVNHLYFAD"
BASE COUNT          667 a          546 c          616 g          954 t
ORIGIN      
        1 cagttcgcct ttcgtattca caagtgcacc cccactattg cccgggttta ctgctgcatc
       61 ggtttgaata aacgattcga tgggcatttg cccgctgata atgcctattc ccctggcttt
      121 tgcactcaca atacctgcgg ttacagttga atttaggttg aaagggttgc ccacggccaa
      181 tacccattcg cctaatttaa gattatcaga atctccccat gaaagggtag ggaggccact
      241 tgcttcgatc ttcaatagcg caatatcggt tgaagggtcg gtgccaataa gttttgcttc
      301 atattcgcgg ttgtcgtgca aggttacctt tatttcgttc gacgattcta taacatgatt
      361 attggtaacc acatacccgt cttctgaaat aattacccct gagcctgttg cttgctgtac
      421 ctggggctgt tcatagtagt agcggtcacc gaaaaagaaa tcaaaaaaag gatttccgct
      481 cgagtaactc tggtcctttt tatatgaggt ttggatatta actactgctt ttaccgaaaa
      541 ctctgccgca taggtaaggt cgggcgaacc ttgcgggttg taactagcat actgaacagc
      601 tgttggttgg gtaaccgtaa caacgttggg cttctcaaaa aactgtgtgt atacgaacat
      661 tcctacaatt ccacctgtaa tggctatcaa cacagtaaaa actactttct gaaatttctt
      721 catggcgtat atacttttaa gattaatata tttgttctta tttttgttcc gatgtaacgc
      781 aaaaatagtt ccgttagttt tgaaaatgtc attgtgtcat atatcgttta acaaaaatta
      841 accactttag ttctaaatag ttaacaagcg ttatttacta agaaaataaa attatgaaac
      901 ttgaattcta caaatatcag ggggctggaa acgattttat tatgattgat aacagggctg
      961 gtttttttga ccacgacaat caagccctta tcgagtcgtt gtgccacaga aggtttggtg
     1021 ttggtgccga tggcctcatc ttgttggaaa atgccagtgg ttacgacttc aaaatggtgt
     1081 attacaacag cgatgggcgc gaagccagca tgtgtggcaa tggaggccgt tgtattgtgg
     1141 cttttgcccg tcagttgggt attatttcaa caagcacatt gtttgttgca tccgacgggc
     1201 ctcatgaagc tttcgttgat gacatggggc ttgtcgacct taaaatgatt gatgtagaga
     1261 aaattgaatc tcttgtcgat ggatatttct tgaatacggg agttcctcat cttgtgcatt
     1321 ttgtcgacga tttgcataca accgacgtgg agctggaagg ccgtcagcta cgatgggacg
     1381 cacggttcca gcctgccgga acaaatgtca actttgtgaa aattgaaggt gaccatttga
     1441 cagttttcac ttacgaacgt ggggttgagg cagaaacgct tgcttgtgga acggggatta
     1501 ccgcttctgc cttgagtgct gcgtttaaaa caggaaagat ttcgggcgaa tacaaaatca
     1561 cagcaaaagg cggagacctg acagttcgtt ttcaacaaga cagtggccgt tatactaaca
     1621 tttggctaaa agggccggct ttactcgtgt atgctggttc tgtaaatatt tagaaattat
     1681 tattacttta tttttgatat tcaattaaat acaccaaaac acatgtcggg agattggttt
     1741 cgtaagttgc ctttgcgtgg ttcgatcatt gcttttttga ttattgctac gtttttgatt
     1801 tattttaaaa ccctttcttt tggcttcctg aatttcgacg atggtcagtt tgttacagaa
     1861 aacgaagtag ttagcagcga tacatcttcg tttgccgatt gtttccggta taaattcggc
     1921 gaacatgatt attttccgct tacttatgca ttcttccgtt tgttacgcct attgttcggt
     1981 ttcaatcctg tagtttttca tgccacaaat ttgctgtttc atattgccaa tgttgtgttg
     2041 gtgttcctcc tgtgtatgaa aatcttaacc cagttttggc ccggaagaaa gaacaacgag
     2101 ttgtcggcag ccattattgc ttttattttt tcctttcatc ctattcatgt tgaatcggtt
     2161 tcgtgggtga tcgacctgaa agacatgctg ttcacattct tctatttgtc ggccttgctg
     2221 ctttattgga agtggatcga tagtcggaag atcgtatttt actacttggc tttcttcctt
     2281 gcgtttttat cattgctttc aaaatcaaca gccattactt tcgtagctgc tctcttttgg
     2341 gtcgattttg ctattggccg taaagggttg aaagagatga ttatagaaaa aattcctttt
     2401 tttctgctaa cccttaccgg tttttatatt ttcgggctgt ttactaatcc agggggggca
     2461 ttgctcggtt tgaccggcgg aatggctgaa ggcgctgcgc cttatttccc ggcctcggtg
     2521 gcttcgttgc cttttttttt acagcgcatt gttattgctt cttttcggct tgggttttgg
     2581 ataatgcatt cggtttttcc tttcgagctg aacttgtttc ttatgcgaag cgtgttgctc
     2641 gaaaagtatg ctttactgct tccgtttatc ccgtttttac tcgttgcggg cttcagcgga
     2701 atatggttgt tgcgcaagaa aacgccattg atgctttatg gtcttgcatt tagaatggtg
     2761 aaccatcttt attttgctga ttt
//
LOCUS       DAOPUW010000223         2781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_37668_length_2781_cov_3.047322, whole genome shotgun sequence.
ACCESSION   DAOPUW010000223 DAOPUW010000000
VERSION     DAOPUW010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37668_length_2781_cov_3.047322"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="PKJ43_04720"
     CDS             <1..1268
                     /locus_tag="PKJ43_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNZ71901.1"
                     /translation="LIMLEYSNIGIFGKMNSGKSTLMNLLTQQETSIADATPGTTADV
                     KMSLMEIHGLGPTRLFDTAGVDEPDVLGEKKRAKTLQLIKECDLVLVVIDPSTSNFTY
                     ENEIIGMARELDKQVVIIFNLFNPFDDDVIVQCKEKLLAGILIPVFSLVAVDVTFRQP
                     LIDFILANFEPDFKPMPLLPFVDANKYYVLIIPMDEETPDGRFLRPQALAMEYITRNW
                     AFPVAYRLDLSKARGAVNESNTEKERFQRFINSFTDKPHAIITDSQAIDIMSRWVSDD
                     ILLTTFSVMMINYTSKGRLAEFYNGVKALETLKPGDKVLIAEACNHSRVSEDIGTVQI
                     PAIISKRFPGVVVEHNFGREFQSNSSLEKYKLIIHCGGCMISHQKLQARLRDLRQVGV
                     PVTNYGIFLSWVQGIAALEKVVNPWLFQK"
     gene            1391..2140
                     /locus_tag="PKJ43_04725"
     CDS             1391..2140
                     /locus_tag="PKJ43_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNZ71902.1"
                     /translation="MRIGLIGFGRTGKAVASVLLASEKCSLMWVYRQKTLLEKRRVAE
                     YLGIENDAEGEVYSSSNTSIDELLDNHPVDIIIDFSSATGIYAYGEAAAKRRIKIISA
                     ISHYSENEKMLLSELAKTTTVFWSPNITLGVNFLLFSAKFLKRIAPWVDIEIIEEHFK
                     GKEGVSGTAVKIAESLDLEKSDINSVRVGGIVGKHEIIFGFPYQTVRLIHESISREAF
                     GNGALFVAENLVDKGSGFYTFEDLMLAYVKE"
     gene            complement(2158..>2781)
                     /locus_tag="PKJ43_04730"
     CDS             complement(2158..>2781)
                     /locus_tag="PKJ43_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71903.1"
                     /translation="TETFHMGNEVRDKRQIYTDDNGWVTQAGDLIISTYVQLLNERKF
                     VPSVSINYTMKTTTGSNTHGRYTDAEMNYFYMAAGKSFEFNSFFIDEIRLAGLLGFYV
                     WQTNKVEMGQDEGKLYELGIQFRKKEWSVFAECGGYNGWDAYEYIDRSSGTNLIDDHN
                     DPLILRTRIERKIKRFELHVEYQTGFRDYLYQSFKLGAVLTFDARKF"
BASE COUNT          794 a          495 c          601 g          891 t
ORIGIN      
        1 tattgattat gttggaatat agtaatatag gtatttttgg gaaaatgaat tctgggaaga
       61 gtaccctgat gaatcttctc acgcaacagg aaacgtcgat tgccgatgct acccctggta
      121 cgactgctga tgtgaaaatg tcgctcatgg aaattcacgg actgggtccg acccggcttt
      181 ttgatactgc aggagttgat gaacccgacg ttcttggtga aaaaaagcga gctaaaactt
      241 tgcagctgat taaagaatgc gatttggttc ttgttgttat cgatccatca accagcaatt
      301 ttacttatga aaatgaaatt attggaatgg ccagagaact cgataaacag gttgtcatta
      361 tttttaatct tttcaacccc tttgatgatg atgtaattgt acagtgcaag gaaaaattgt
      421 tggcggggat tctcattcct gtgttttcat tggttgctgt agatgtaact tttcggcagc
      481 cacttatcga ttttattctg gcaaattttg aacctgattt caagcccatg cctttgctgc
      541 catttgttga tgcgaacaag tattatgtgt taataattcc tatggatgaa gaaacccctg
      601 atggccgttt tcttcgtcct caggctttgg caatggaata tattacccgt aactgggcgt
      661 ttccagtagc ataccgtctc gacctatcaa aagctcgagg tgcagtgaat gagtcgaata
      721 ccgagaaaga acgttttcaa aggtttatca atagttttac ggataagcca catgcaataa
      781 ttactgattc tcaggctatt gacattatgt cacgctgggt ttcagatgat attttgctta
      841 cgaccttttc ggtgatgatg attaattaca cgagcaaagg acgcttagct gagttttata
      901 atggggttaa agccctcgaa accttaaagc ctggagataa agtgttgatt gccgaagcct
      961 gcaatcattc aagggtaagc gaagatatcg ggacggttca aattcctgct attatttcaa
     1021 aacgctttcc tggtgttgtt gtagagcata attttggtcg ggaatttcaa tcgaacagtt
     1081 ccctcgaaaa gtataaactg attattcatt gcggaggatg catgatcagt catcaaaaat
     1141 tacaggcccg attacgtgat cttcggcaag taggagtgcc agttactaat tatgggattt
     1201 tcttatcatg ggtacaaggt attgcggcac tcgaaaaagt tgttaaccca tggttgttcc
     1261 agaaataaag ttattaatct attaatgatt tgatgttcag aatataaagc catttttgat
     1321 tttcattatt ttttgtaatt ttatagaatt gatcaaataa atcataatat taaatataaa
     1381 agatagtgaa atgaggatag gtttaatcgg gtttggccgg acaggaaagg ctgttgcttc
     1441 agttttgctt gcaagcgaga aatgtagtct aatgtgggta tataggcaaa agactctttt
     1501 agaaaaaaga cgtgttgcgg agtatcttgg cattgaaaac gatgctgaag gagaagtata
     1561 ttccagttca aatacttcaa ttgacgagct cctcgataat cacccggtcg atataataat
     1621 cgatttctct tcagcaacgg gtatttatgc ttacggagaa gcagcagcca aaagaagaat
     1681 aaaaataatc tcagctatct cgcattatag cgagaacgaa aaaatgcttt tatcagaact
     1741 tgcaaaaacg actactgtgt tttggtcgcc gaacattacc ttaggcgtta actttctgtt
     1801 gttttcggca aagtttctta agaggattgc cccctgggta gatattgaaa taattgaaga
     1861 gcattttaaa ggaaaagaag gggtttcggg gactgctgtt aaaattgccg agtcgctcga
     1921 tttggaaaaa tcagatatta attccgtccg tgttggggga attgttggaa agcacgaaat
     1981 aatttttgga tttccttatc aaaccgttcg ccttattcat gagtcaatat cgcgtgaggc
     2041 ttttgggaat ggtgcgcttt ttgtcgccga aaatcttgtt gataaaggaa gtggtttcta
     2101 tactttcgaa gacctgatgc tggcttatgt gaaagagtaa atcccgttac ttctttttca
     2161 aaattttcgt gcatcgaaag ttaaaacggc tccaagttta aatgattggt agaggtagtc
     2221 gcgaaaacct gtttgatatt caacgtgtaa ctcaaatctt ttgatttttc tttctattcg
     2281 ggtacgtaag attagcgggt cgttatgatc atcgatcagg ttggtgccgc tggagcggtc
     2341 gatatattcg taggcatccc atccattata tcccccgcac tctgcaaaaa ccgaccattc
     2401 tttcttcctg aattggatgc ctaattcgta taattttcct tcgtcttgcc ccatttctac
     2461 tttgttggtt tgccaaacat aaaaacctaa gagtccagcc aaccgtattt cgtcgatgaa
     2521 aaaactgttg aattcgaaac tttttccggc cgccatgtag aaataattca tttcagcatc
     2581 ggtataccga ccatgggtat tgctgccagt agtagttttc atggtgtaat tgatagaaac
     2641 cgacgggaca aattttcttt cattcaaaag ttgaacataa gtgcttataa ttaggtcgcc
     2701 agcttgtgtt acccaaccgt tgtcatcggt gtaaatttgt cgtttatcac gcacctcgtt
     2761 gcccatatga aatgtttcgg t
//
LOCUS       DAOPUW010000224         2772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_37865_length_2772_cov_2.784321, whole genome shotgun sequence.
ACCESSION   DAOPUW010000224 DAOPUW010000000
VERSION     DAOPUW010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37865_length_2772_cov_2.784321"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..287)
                     /locus_tag="PKJ43_04735"
     CDS             complement(39..287)
                     /locus_tag="PKJ43_04735"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ71904.1"
                     /translation="MDFDILIIGSGPGGYVAAIRASQLGLKVGVVEKENIGGVCLNWG
                     CIPTKSLLKSAQAYEYAVHAESYGVSLPGKLPPIWGKW"
     gene            complement(499..1593)
                     /locus_tag="PKJ43_04740"
     CDS             complement(499..1593)
                     /locus_tag="PKJ43_04740"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNZ71905.1"
                     /translation="MKHFLLISLSALLLWSCGNKNQFSITGKVEPAMDGKMILFGFKD
                     GQPVPVDTTTIEKGKFSFKGEVEIAEIRLLAIDGQNNFAAQLFIEPAKISMTVYPDSF
                     ETNVIKGSKSQDIFQKYIDEMITFSEKAEGLKSRFAQAQATTNQEEMAAVQYEYETMI
                     ENNMLFARNFVKEYKSSPVAAYVYMQTFLQEDQIEAIDSMLEVFKPIEKSEFITVIKD
                     YAEQIRPFSKGAIAPDFTLAKADGSSFTLSSLKGKYVLVDFWASWCKPCMVDMPNVIE
                     LYKTYKDKGFEIVGVSLDREKEAWLNTIKTPGMEWIHVWDMEGDVPGGVATKFGVTGI
                     PHTVLLDKEGKIIEINLRSEGLQAKLSQLL"
     gene            complement(1604..2725)
                     /gene="meaB"
                     /locus_tag="PKJ43_04745"
     CDS             complement(1604..2725)
                     /gene="meaB"
                     /locus_tag="PKJ43_04745"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNZ71906.1"
                     /translation="MARKDNHHIENSPDFIGLKVNSGIPQPPSVNPNSPHRNHSRKRK
                     ELSVDEYVSGILSGNRMILSRAVTLIESVKPEHQAIAQEIILKCLPHSGKSIRIGITG
                     VPGAGKSTFIEALGKHITSHQGKLAVLAIDPSSERTKGSILGDKTRMEELANDPNAYI
                     RPSPSAGSLGGVARKTRETIILCEAAGFNTIFIETVGVGQSETAVHSMVDFFLLIQIA
                     GAGDELQGIKRGIMEMADGIVINKADGNNVEKAELAQAQYRNALHLFPTPPSGWHPNV
                     LKCSSINNSGIGEIWQMVTEYISFTQNNNYFDERRAQQSKYWMYESINETLKNSFYTH
                     PEIKKEIITYEDAVLKEKISSFVAAKDLLSIYFKNKTNI"
BASE COUNT          704 a          590 c          574 g          904 t
ORIGIN      
        1 gcctttgctc attccttcgg ccacgtcgcg gctacgtttt accattttcc ccaaatcggg
       61 ggtaacttgc ccggtaagct aacaccataa ctttcggcat ggacggcata ttcgtaggcc
      121 tgggcacttt tcagcagcga tttggtagga atacagcccc agttaaggca aacgcctcca
      181 atgttttctt tttctactac accgaccttc aatcctaatt gtgaagccct aatcgcggca
      241 acatagcccc ccgggccact gccaattatc aatatatcaa aatccatttt tagagttttt
      301 agtcacaaaa tactaaataa acgaaatagt tgtcaataag tttatccata ggtttataaa
      361 ttggtgtttt tcaagggttg aaaggttctt cctgaattgt agggggtgtt tgttatttac
      421 taagtctaat gtgttttata aaaaaaccac cgcagggtat tgcgatggtt ctttctattt
      481 gatttaaatg attttttctt ataaaagctg gctaagtttt gcttgcaaac cctcgcttct
      541 tagattaatt tcgataatct ttccttcttt gtcgagaaga acagtgtggg gaatgccggt
      601 aactccgaat ttagtggcaa ctcctccggg aacatcgcct tccatatccc aaacatggat
      661 ccattccatg ccaggagttt tgatggtatt cagccaggct tctttctcac ggtcgagtga
      721 aacgccaaca atttcgaacc ctttatcttt atacgtttta taaagttcga ttacattcgg
      781 catatcaacc atacaaggtt tgcaccatga tgcccaaaag tctactaaaa catattttcc
      841 tttcagagaa gaaagcgtga aagagcttcc gtcggctttt gctaatgtga aatcgggagc
      901 gattgcaccc ttgctgaagg gcctgatctg ctcggcataa tctttaatta cagtgatgaa
      961 ctctgacttt tcaatgggtt tgaaaacttc aagcatcgag tcaatggctt caatctggtc
     1021 ttcctgaagg aatgtttgca tgtaaacata tgcagctaca ggcgacgatt tgtattcttt
     1081 aacgaaattg cgggcgaaaa gcatgttgtt ttcaatcatc gtttcgtatt cgtactgaac
     1141 ggcggccatt tcttcctgat tagtggttgc ctgagcctga gcaaaacgag atttcaagcc
     1201 ttcggctttt tcagagaaag taatcatttc gtcgatgtat ttctggaaaa tatcttgcga
     1261 ctttgatcct tttatcacat tggtttcaaa actgtcggga taaacagtca tcgatatttt
     1321 tgcaggttca atgaagagtt gtgcagcaaa attgttctgg ccgtcgatgg cgagtaaacg
     1381 gatttctgca atttcaactt cccctttgaa cgaaaacttc cctttttcga tggttgtggt
     1441 gtcaacagga acaggttgtc cgtctttaaa tccgaaaagt atcattttac cgtccatggc
     1501 aggttcaact tttccggtta tactgaattg gtttttattt ccacacgacc acagcaagag
     1561 ggcagacagg gaaatcagca aaaaatgttt cataattctc tatttaaata tttgttttgt
     1621 tcttgaaata tatagacaat aagtctttag cagcaacaaa agaactgatt ttttctttca
     1681 aaaccgcatc ttcgtaggta attatttcct ttttgatttc aggatgggta tagaatgaat
     1741 tcttcaatgt ttcgttaatg ctttcgtaca tccagtattt tgattgctgg gcgcgacgtt
     1801 cgtcgaagta gttgttattc tgtgtaaatg aaatatactc ggttaccatt tgccaaattt
     1861 ctccaattcc actattgttt atcgaagagc atttcagcac gttaggatgc catcccgaag
     1921 gtggagtggg gaaaaggtga agggcattac ggtattgcgc ctgagcaagt tcggcttttt
     1981 ctacattgtt cccgtcagct ttatttataa caattccatc ggccatttcc attatgccgc
     2041 gcttgattcc ttgcagttcg tcgccagctc cggcaatttg tatcaacagg aaaaaatcga
     2101 ccattgaatg aactgctgtt tcgctttgtc caacgccaac ggtttcgata aatattgtat
     2161 tgaatcctgc tgcttcacaa agaattatag tttcgcgggt ttttcgggcc acgccgccca
     2221 acgaaccggc cgagggcgaa ggccgtatat atgcattcgg gtcgttcgct aattcttcca
     2281 tccgggtctt atcgcctaga atactccctt tggtgcgttc actgcttggg tcgatggcca
     2341 gtacggcaag cttaccctga tgacttgtta tatgcttacc caatgcttct atgaaggtgc
     2401 tttttcctgc acccggaact ccggttatgc caatcctgat cgattttcca ctatgaggaa
     2461 ggcatttcag gattatttcc tgggcgatgg cctgatgttc tggttttaca ctttcgatga
     2521 gcgttactgc tcggctaagg atcatccggt tgcccgacaa aatgcccgaa acatattcat
     2581 ctacagaaag ttcttttcgt tttcttgaat gattacggtg cggagagttg ggatttaccg
     2641 agggtggttg aggaatccca gaattcactt taagcccaat aaaatcagga ctattttcga
     2701 tatgatgatt gtcctttcgt gccattttga attttttttt gcgaagatac atcttttgcg
     2761 gctaaccgtt ca
//
LOCUS       DAOPUW010000225         2769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_37927_length_2769_cov_3.813191, whole genome shotgun sequence.
ACCESSION   DAOPUW010000225 DAOPUW010000000
VERSION     DAOPUW010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37927_length_2769_cov_3.813191"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..169)
                     /locus_tag="PKJ43_04750"
     CDS             complement(41..169)
                     /locus_tag="PKJ43_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71907.1"
                     /translation="MAKKRCKKEDYLPPEEPKYQCKKCGRLAKKENQVCDPKKYKL"
     gene            complement(162..1607)
                     /locus_tag="PKJ43_04755"
     CDS             complement(162..1607)
                     /locus_tag="PKJ43_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNZ71908.1"
                     /translation="MDIKHTSDILSSLFSMVFFNEKPTSIEKLPGSGSDRVYYRLKNE
                     NWSAIGAFNENVKENEAFFSFTNTFLEIRIPVPTLYAIAPDRQHYLISDLGNETLYDL
                     IVSKRKSGNCGDALCAMKEVLAGLIMIQLDGGDTVDFSKCYPSEAFDRRAIMWDLNYF
                     KYQFLKISTVPFDEQCLEDDFERLADYLLQAPSHFFMYRDFQSRNIMMVDNKPFFIDY
                     QGGRKGPLQYDLASLLFSPKTGLNSVQREVMLEYYIKLLESYFSFDRKEFIDFYYAFA
                     LARIMQALGAYGFRGIYEHKANFRSSIPLAIENLKWIKDNNHITIELPEIYRIIDSLA
                     QSEWAQKFDLPANKLTVRVTSFSYKKGIPVDPSENGGGFVFDCRGLPNPGRLPEFKNQ
                     TGLDEPVIQYLEQYDEVKNFQQHVRDMIKISLDEYLSRGFNHLMVSFGCTGGQHRSVY
                     NAESFARWLENNFAVNVVLLHTEIKTIKANG"
     gene            complement(1638..2513)
                     /locus_tag="PKJ43_04760"
     CDS             complement(1638..2513)
                     /locus_tag="PKJ43_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNZ71909.1"
                     /translation="MFTSENVEVPVFSWSEKILPWIMAHGIKIVFIIVVTLILNRFVN
                     RFITRIVRIAVVADKYSSKEAEEKRENTLIHIFTFALRILLWSLAILTILQEFGVKVG
                     ALLGAAGIVGVALGFGAQSLVRDVISGLFIIIENQYRIGDVIDVGTVNGTVEDITLRK
                     TTLRDIDGTTHHIPHGEIKIVSNKSKDFARINLDVGVAYDSNLEHVIEVVNRVGNELA
                     EDPAWKDMITTPPQFLRVNKLGDSAIEIKILGETIPLKQWEITGELRKRIVIAFNKEN
                     IEIPFPQRVIHSPNK"
BASE COUNT          845 a          632 c          490 g          802 t
ORIGIN      
        1 agctatcaaa ttttaaagaa caataaatga atgtgcatca ctaaagttta tactttttag
       61 ggtcgcacac ctgattttct ttcttggcaa gccttccgca ttttttgcat tggtattttg
      121 gttcttcagg aggcaaataa tcttcttttt tacatctttt tttagccatt tgctttaatg
      181 gttttaattt cggtatgcaa taaaaccacg ttcaccgcga aattgttttc gagccaacgg
      241 gcaaaacttt cggcattata aaccgaccgg tgctgacctc cggtgcaacc aaaactaacc
      301 atcaaatggt tgaagccacg ggaaagatat tcatccaatg aaatttttat catatcgcga
      361 acatgctgct gaaaattttt cacctcatca tactgttcaa gatactgaat aacaggctca
      421 tctaagccag tttgattttt aaattcagga agtcgtccgg gatttggcag cccccggcaa
      481 tcgaatacaa aaccgccacc attctccgaa ggatcaacag ggatgccttt tttatacgaa
      541 aagctggtta ccctaacggt aagtttattc gccggcaaat cgaacttttg agcccattcc
      601 gactgcgcta acgaatctat aattctatat atttcaggca gttctatggt tatatggtta
      661 ttgtctttga tccatttcaa gttttcgatc gctaatggaa tgctgcttcg aaaatttgcc
      721 ttatgctcat aaattcccct aaacccatat gcaccaaggg cctgcattat tcgagcaaga
      781 gcaaaggcat aataaaaatc gataaattct ttgcggtcga aagaaaaata actctcaagt
      841 agctttatgt aatactcgag cataacctcc cgctgaacag agtttaagcc agtctttggg
      901 ctaaaaagca gcgaggccag gtcgtattgc agaggcccct ttcttccccc ttgataatca
      961 atgaaaaatg gtttattatc gaccatcata atattcctcg actggaaatc gcggtacata
     1021 aaaaaatgag aaggagcttg tagcaaataa tccgcaagtc gttcaaaatc atcttcaagg
     1081 cactgttcat cgaaaggaac agttgatatc ttcaaaaatt ggtatttaaa atagttaagg
     1141 tcccacataa tggcgcgcct gtcgaatgct tcgctgggat aacatttcga aaaatcgaca
     1201 gtgtcgcccc catcgagctg tatcataatc aagccagcca acacttcttt catggcacaa
     1261 agagcatcgc cacaattccc tgattttctt ttgctcacaa tgagatcata caacgtttca
     1321 ttgccaaggt ctgagataag gtaatgctgc cgatcgggag ctatggcata cagtgtcgga
     1381 accggaatcc taatctcgag aaaagtattg gtgaaactaa agaacgcttc gttttctttc
     1441 acattttcat taaacgctcc aatagccgac cagttttcat ttttcagccg ataataaacc
     1501 ctatcggaac ccgaccccgg gagtttctct atgcttgttg gcttttcatt aaaaaagacc
     1561 atactgaaaa gggatgagag aatgtcagat gtatgcttta tgtccataat agttttacta
     1621 aggttctatg gtcgttttta cttgtttggc gaatggataa cccgctgggg gaatggaatt
     1681 tctatatttt ctttattgaa agcaatcaca attcttttac gcaattctcc ggtgatctcc
     1741 cactgtttta acggaattgt ttcgcctaat atttttattt caatggctga atcgcccaat
     1801 ttattaaccc tcaggaattg cggaggcgtt gttatcatat ctttccatgc ggggtcctcg
     1861 gccaactcat ttcccacacg atttaccact tcaataacat gctcaaggtt agaatcataa
     1921 gctacaccga catctaaatt gatgcgggca aaatctttcg atttgttcga aacgatttta
     1981 atttccccat gaggtatatg gtgcgttgtt ccatcgatat cgcgtaaagt agtttttcgc
     2041 aaggtaatat cttcaacggt tccgttgacg gttccaacat cgataacatc gccaatacga
     2101 tactgatttt caatgataat gaacaaacca gaaatcacat cgcggaccaa cgactgagcg
     2161 ccaaacccca acgccacacc cacaatacct gcggccccca acagagctcc tacctttacg
     2221 ccaaactcct gaagtatggt gagtatagcc aacgaccaca aaagaatccg caaggcaaac
     2281 gtaaatatat ggatcagcgt attttcccgc ttttcttcag cctctttcga cgaatacttg
     2341 tcggcaacca ctgcaatgcg cacaatacgg gttatgaacc gattgacaaa cctgttgaga
     2401 ataagtgtaa caacgataat aaatacaatc ttaatcccat gagccataat ccatggaaga
     2461 attttctcgc tccaactgaa aactggcact tcaacatttt cactcgtaaa catattcgtc
     2521 aattaaacat taataatact atatttcaga atcctacaaa ctataagtag ctttatctaa
     2581 atatcgaaat aactcttatt taggcaaaaa caaataggcc aatacaacgg caaacaccaa
     2641 agccggtaac atattgacta cttttatttt ttttatttcc atgatcgaaa agccaaggcc
     2701 caccagcatt aagccaccag cagcagacat ttcgtcgacc acaaccgaag gcataaggtt
     2761 ccccagcca
//
LOCUS       DAOPUW010000226         2759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_38139_length_2759_cov_2.855769, whole genome shotgun sequence.
ACCESSION   DAOPUW010000226 DAOPUW010000000
VERSION     DAOPUW010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38139_length_2759_cov_2.855769"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2759)
                     /locus_tag="PKJ43_04765"
     CDS             complement(<1..>2759)
                     /locus_tag="PKJ43_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNZ71910.1"
                     /translation="GYNFLGVRLYPAIDPPIITVSTSYIGANSEIIESQITEVLERSI
                     NGIEGVKSISSSSSMGSSRITVEFELGSDLEKAANDVRDKVSQATRRLPLDLDAPPTV
                     TKADANAEPIMMLIAQSTKMNAIELSEYADNVLLEKFQTIPGISSVSIFGEQKPAMRL
                     WIDPVKLAARGLTISDVTSALSRENVETPGGKIRGEGTELTIRTYGRLTSEEDFNNLI
                     IKQNNNQVIRFSDVGEAVLGPQDEESGATINGANGVTIAMIPLPGANTIEIADEFYKR
                     LEQVKENLPEGMELTMGRDSSIFVRQSVHDVLETLLIAIVLVVIIIFLFFRNWIIAIR
                     PLIDIPVSLIGTFFVMYIMGYSINVLTLMGIVLATGLVVDDGIVVTENIFKRIERGMN
                     KWRAAAEGTREIFFAVVSTSLTLAIVFIPVIFLEGFTGRLFREFGIVMASAILISAIV
                     SLTLTPVLNVKLGGSASHHSAFYNKTEPFFVGMENGYRKALKLFIGHKWASFLIMILC
                     ILLIVFIGGSLKSELAPLEDHSFIRTSITAPEGTEFSTTQKITNSIADMQLETIPEIR
                     YVLTRYGGWGGSSGNSGNIMTMLDEPDSRERSQQEIYDELTAKYSAISGARVIASQEP
                     TIATAFSRGLPVQFVLQNIDFQKIRDVLPRFLEEARNSEVFSVVDSDLKFNKPELNII
                     VDRLKATSLGVNIRDITDALNMAFSGGRTGYFLRNNKQYYVVMQVERENRDDPADISK
                     LYVRNNKGEMIQLDNLVKIEENSNPPTLYHFNRYKSATISANLAKGYSLGEGIDEMNR
                     IAKGLLDSSFETDLSGPSRDFAESGSNIFFALALALLLVYLILAAQFESFRDPFIIML
                     TVPMAIAGAMLSLWIFGHTLNIFSEIGMLLLIGIVTKNGILIVEFANQKREAGLSIKD
                     AAFEA"
BASE COUNT          825 a          630 c          568 g          736 t
ORIGIN      
        1 cagcttcaaa cgcggcatct ttaatagaaa gcccggcttc gcgtttttgg tttgcaaact
       61 caacaatcaa aattccgttc ttagttacaa taccaataag aagcaacata ccaatttcgg
      121 agaagatgtt cagggtgtgc ccaaaaatcc aaagcgaaag cattgcacca gcaatagcca
      181 ttggcacggt aagcataata atgaacgggt ctctgaaact ttcgaactga gccgccaaaa
      241 tcaaatacac aagcaacagg gctaaagcaa gagcaaagaa aatattggag ccactctcag
      301 caaagtcgcg cgatggtcct gacaaatcgg tctcaaacga cgaatcgaga agccctttag
      361 caatgcggtt catttcgtcg atcccctcgc ccagcgagta acctttggcc aggttggcag
      421 aaatggtagc cgatttgtaa cgattgaaat ggtaaagggt tggaggatta ctattttctt
      481 caatttttac caggttatcg agctgaatca tttccccttt gttatttcgc acgtaaagct
      541 tcgaaatatc tgcgggatcg tcgcggtttt cgcgttcaac ctgcattaca acataatact
      601 gcttgttgtt ccggagaaaa tacccggtac gtcccccgct gaaagccata ttaagtgcat
      661 cggtaatatc cctgatattt acaccaagac ttgttgcctt aagcctgtcg acaatgatgt
      721 tcaattcggg tttgttaaat ttaagatctg aatcgaccac gctgaacact tcgctatttc
      781 tggcttcttc caagaaacga ggcaaaacat cgcgaatttt ctgaaaatca atattttgca
      841 acacaaactg aacgggcaag cctcgtgaaa aagcagtagc aatagttggt tcctgacttg
      901 caataacccg ggcacccgaa attgcactat attttgcggt cagttcatcg taaatttctt
      961 gttgagatcg ttcacgagaa tcaggctcat caagcatagt cataatatta cctgagttac
     1021 cagagcttcc tccccaacct ccatagcgtg tcaatacgta acgtatttcc gggattgttt
     1081 ccagctgcat atcggcaatg gaattggtaa ttttctgagt agttgagaac tctgtccctt
     1141 caggtgcggt aattgatgta cggataaaac tatggtcttc gagcggagcc aattcgcttt
     1201 ttaaactacc cccgataaag acaatgagca ggatacaaag gatcatgatt aaaaatgaag
     1261 cccatttatg accgataaac aacttaagtg cttttcgata gccgttttcc atccccacaa
     1321 agaatggctc ggttttatta tagaatgccg aatgatgcga agctgaacca ccaagtttta
     1381 cgttaagaac tggggtaagt gttagcgaaa caatggctga aattagaata gcactagcca
     1441 ttacgattcc aaattcgcgg aaaagccgtc cggtaaaccc ttcaagaaaa ataaccggaa
     1501 tgaaaacaat ggcaagagtc aaagaagtag aaacaaccgc aaagaaaatt tcgcgggtac
     1561 cttctgcggc agcacgccac ttgttcatac cccgttcaat acgtttaaaa atattttcgg
     1621 ttaccacaat cccgtcgtca acaacaagcc cagtggccaa gacaatcccc ataagagtca
     1681 gcacgttgat ggaatacccc ataatataca tcacgaaaaa agtgcctatc agcgaaaccg
     1741 ggatatcgat taatggtcgt atggcaataa tccagttacg gaaaaagagg aaaataatta
     1801 tcacaaccaa aacaatggct atgagcaatg tttcaagaac atcgtgaacc gactgcctga
     1861 caaatattga actatcacgc cccatggtga gctccattcc ttcaggaagg ttttctttta
     1921 cttgttcgag gcgtttgtag aactcatcgg caatttcaat ggtattggct cccggtaaag
     1981 ggatcatggc tatagttacc ccattggctc catttatcgt tgcccccgac tcttcatcct
     2041 gtggaccaag aaccgcttct ccaacatcgc tgaaacggat tacctgatta ttattttgct
     2101 ttataatcaa attattaaag tcttcttcgg aagttaatcg gccatatgta cggatggtca
     2161 attcggtccc ttcacccctg attttacctc ccggtgtttc cacattttca cggcttaacg
     2221 cagacgttac atcagaaata gtaaggccgc gggcagcaag ttttaccggg tcaatccata
     2281 gacgcatagc tggtttttgt tcaccaaata ttgaaacgga actgatgccc ggaatggttt
     2341 gaaatttttc aagaagcaca ttgtcggcat attcacttaa ttcaatcgcg ttcatcttgg
     2401 tgctttgtgc tataagcatc atgatgggtt ccgcattggc atcggccttg gtaaccgttg
     2461 gcggggcatc aagatcgagg ggaaggcgcc gtgttgcttg cgatacttta tcgcgtacat
     2521 cgttagctgc tttttcaagg tcggaaccca gctcaaattc aacagtaata cgactcgatc
     2581 ccatagatga agaagaggaa attgacttca ccccttcaat cccattgata gaacgttcga
     2641 gcacttcagt aatctgcgat tcaataattt cggaattcgc tccaatatac gaagtggata
     2701 cagtaataat aggtgggtct atcgctggat aaagcctaac tcccagaaaa ttataccca
//
LOCUS       DAOPUW010000227         2745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_38406_length_2745_cov_3.877323, whole genome shotgun sequence.
ACCESSION   DAOPUW010000227 DAOPUW010000000
VERSION     DAOPUW010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38406_length_2745_cov_3.877323"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PKJ43_04770"
     CDS             <1..348
                     /locus_tag="PKJ43_04770"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ71911.1"
                     /translation="KDYDIRINILKAEVMPGKRGSLLLEMEARQSNLDKGIAYLEAHN
                     VECQPLDKRIQFNSEKCISCGNCTAVCFAGALTMNPVTWKMEFNKELCVACELCVKAC
                     PLRLFDIDFRDGN"
     gene            367..1143
                     /locus_tag="PKJ43_04775"
     CDS             367..1143
                     /locus_tag="PKJ43_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HNZ71912.1"
                     /translation="MTPEEKYGFGPRFYRSQMGHGRFKAFEVSYKDSDLWIGVDSESF
                     NPDMVDFARSSLIALRQELEEFILRCPQFRSSFSPIEIGSHAPEVAISMCNAGKIAGT
                     GPMAAVAGAFSEVIGKALKKKFKVEEIIVENGGDIYLDIQKDIVLSVYAGNSPLSGKI
                     GVQIPQELSPLGVCTSAGKIGPSVSFGRADAVMVACYNTALSDALATALGNLVTSPED
                     IKTVIEKAQKYSEIISAIIICDDKIGVSGQLSICPINSFP"
     gene            complement(1140..1910)
                     /locus_tag="PKJ43_04780"
     CDS             complement(1140..1910)
                     /locus_tag="PKJ43_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71913.1"
                     /translation="MMKALKVIIFSVVTIAVVLYLVVITAAPLRKVAELNRLATSDSV
                     FMNKYTAIATYPKLLPLVKEKAHLQAQIEMAESDSIGLVINVKEKKASLMLKGVEIHS
                     SLIKELNIDPVFKGIESPAYRKMFTNPIHNVGEYSSVIKEPIVYKKAPKDTIEAMKML
                     TLPVAPEPEPAYVNYDLQYGIKLILVQDKWETEADLIEKKKYTDDMKKIRIHNSVGLL
                     KNLKEPAYIPVISAVMDGKEVRAIYRALPQKANIVFIF"
     gene            complement(1924..2682)
                     /locus_tag="PKJ43_04785"
     CDS             complement(1924..2682)
                     /locus_tag="PKJ43_04785"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNZ71914.1"
                     /translation="MNDQNLDVSENLAENTLPESVDIQAQTQKKPFSKTIFGSIVLST
                     VFVIVVLFSVLILPNFLQKIAIPISKLGVDSFKNYEEAMAPDKAILEKSIAKLQKQLN
                     DFVPESFYMIINSTDNEFALMKAGQLIRQGQCSTGSYVVLEDHKQRKYLFKTPKGRRK
                     VLSKVKDPVWTKPNWAFIEEGLPVPPANHPSRFEEGVLGDYALKLGDGYMIHGTIWQR
                     YLGLPVTHGCVRLNDADLEVVYKSLDKGAFVYIY"
BASE COUNT          811 a          581 c          549 g          804 t
ORIGIN      
        1 aaagactacg acatccgtat taacatttta aaagctgaag taatgcctgg aaaacgaggc
       61 agtctgctcc ttgagatgga agcacggcag agcaacctag acaaggggat tgcttacctt
      121 gaggctcaca atgtagagtg ccaacccctt gataaaagaa ttcaatttaa cagcgaaaag
      181 tgtatcagtt gtggaaactg cactgcagta tgctttgcgg gagcgctcac aatgaacccg
      241 gttacgtgga aaatggaatt taacaaggaa ctttgcgtag cctgcgaact ttgcgtaaaa
      301 gcttgccctt tacgtctttt cgatatcgat tttagagacg gaaattaacc caagaaacaa
      361 aagcggttga ctccggaaga aaaatacggg tttgggccta ggttctaccg cagccaaatg
      421 ggccatggtc gcttcaaagc gttcgaagtt tcatacaaag attccgattt gtggattggt
      481 gtagattctg aaagctttaa ccccgacatg gtcgattttg cccgctcaag cctcattgca
      541 cttaggcaag agctcgaaga atttatcctt aggtgcccac aattccgcag ctctttctct
      601 cctattgaaa ttggctctca tgcgcccgaa gttgcaattt caatgtgcaa tgccgggaaa
      661 atagcaggaa ctggacctat ggcggctgtt gctggtgctt tttccgaagt tataggaaaa
      721 gcattgaaga agaaattcaa agtcgaagaa atcattgttg aaaatggcgg cgatatttac
      781 cttgacatcc agaaagatat tgtactatcg gtttacgcag ggaattcgcc attatcgggc
      841 aaaattgggg tacaaattcc gcaggaacta agtcctctgg gggtatgcac ttcggcagga
      901 aaaattgggc cttcggtaag ttttggccgc gctgatgctg taatggtcgc ctgctataac
      961 accgctcttt ccgatgcctt agccacagca ttaggcaatt tggttacatc gccggaagat
     1021 ataaaaacgg ttatcgagaa agctcaaaaa tattccgaaa tcatttccgc aataatcatt
     1081 tgtgacgata aaattggcgt ttcaggccaa ttaagtattt gcccgataaa ttcctttccc
     1141 taaaaaataa aaacaatatt agccttttga ggtaaagccc tgtatatggc ccgcacttct
     1201 tttccgtcca ttactgctga aataacagga atatatgcag gctctttaag gtttttaagc
     1261 aatcccacac tgttgtggat acgaatcttt ttcatgtcat cagtgtattt ctttttctcg
     1321 atcagatctg cctcagtttc ccacttgtcc tgaactaaaa taagtttaat accatattga
     1381 aggtcataat taacataggc aggttctggc tccggagcga ctggcaaagt caacattttc
     1441 atcgcttcaa ttgtgtcttt aggagctttc ttatacacaa ttggctcttt aattaccgat
     1501 gaatactcgc caacattgtg aatagggttc gtaaacatct ttcggtatgc aggagattca
     1561 atccctttaa aaacaggatc gatattcaac tcttttatca atgaagaatg aatctctacc
     1621 cctttaagca ttagggaagc tttcttttct tttacattga taaccaaccc aattgaatca
     1681 ctctcagcca tttcgatctg cgcctgaaga tgggcttttt ctttcaccaa aggaagtaat
     1741 ttggggtagg tagctatagc ggtatattta ttcataaaaa ccgaatcgga agttgcaagg
     1801 cggttcaatt ctgctacttt acgcaaaggc gcagctgtta ttacaactaa gtacaacacc
     1861 acagcaatag taacaacaga aaatataata acttttaatg ctttcatcat ctaaatcagt
     1921 taattaataa atataaacaa atgcaccttt atcgagcgat ttatacacca cttcgaggtc
     1981 agcatcgttg agacgcacac agccgtgggt taccggcagc cctaaatacc gttgccaaat
     2041 agtcccgtgt atcatatacc catcgccaag tttaagcgca taatccccca atacaccttc
     2101 ttcaaaccgg gaaggatggt ttgctggggg tacaggcaac ccttcttcta taaaggccca
     2161 attcggcttt gtccataccg ggtcttttac tttgctaagc actttacggc gacctttggg
     2221 tgttttaaag agatacttcc gttgtttgtg gtcttcaaga accacataac tcccggttga
     2281 gcattgcccc tgacgaatga gttgcccggc tttcatcagc gcaaattcat tatcggtact
     2341 atttataatc atgtaaaacg attcgggaac aaaatcgtta agctgctttt ggagtttagc
     2401 aatgcttttt tccaaaattg ccttatcggg agccatggct tcttcgtaat tcttgaacga
     2461 atcaacaccg agcttcgata ttggaattgc aattttctgt aaaaaattag gaagaattaa
     2521 taccgaaaaa agcacaacaa taacaaatac agtgcttaac acaatggatc caaaaatggt
     2581 tttagaaaaa ggttttttct gagtttgagc ttgaatatct acgctttctg gaagcgtatt
     2641 ttctgccaaa ttttctgata catccaaatt ctgatcattc atattatggt aatttcatta
     2701 tttcacttaa gggcctcaac gaaccaacaa tggtaactgt tgtcc
//
LOCUS       DAOPUW010000228         2738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_38551_length_2738_cov_4.050690, whole genome shotgun sequence.
ACCESSION   DAOPUW010000228 DAOPUW010000000
VERSION     DAOPUW010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38551_length_2738_cov_4.050690"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1090
                     /locus_tag="PKJ43_04790"
     CDS             98..1090
                     /locus_tag="PKJ43_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNZ71915.1"
                     /translation="MKTQLLSLILICISNVVFAQNQPLSQQADSVVSNADQLPLDELL
                     SENDSLAEAAVLKPDSVNKGSGTEKIDTVHIRVGKHAIEIVTDDDRTQIDVDKLEEFG
                     KHIEQSEIERAERKAEKHKKKKFNGHWGGIDFGGNQLLNTTYPESLYPAGTPEFMITA
                     PEKSFEVNVNLFEYSFGFSSYVGLVTGLGLNFNDYKFRNRYTLVKDDNGVIQPQSLPE
                     GDFRLSKLSTTFIHAPLMLEVQIPGQYDHKRIFIAGGVIGGVKIHEHVKTKIGSEKKR
                     DNGNYNVAPIRWGYTARVGFEDVGIFATYYNTKFFEKGLGPEATPFTVGLAFAF"
     gene            1206..>2738
                     /locus_tag="PKJ43_04795"
     CDS             1206..>2738
                     /locus_tag="PKJ43_04795"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNZ71916.1"
                     /translation="MGRIVPFFWLVFFVIPATFGSPSDFVNFNTGNGKLGHNTVEAVV
                     RDTFGYLWVGTNYGLNRLDGYQTINFLSDPEDSSSISSNFILSLFVDTENNVWVGTLG
                     GGLNMYDRNTGRFKRFVTTDKPGGIGSLNISEIIEDLNGDLWIGTNGNGVNRFNKKTG
                     VFSHYNIEQYDPEHRKNSNVTKLFRDCEGNIWVGMNQSEVFKIDPKTEKITFHGLTRS
                     DNGFRDIGAILGFIQEGSGRLLISGRSGNIYHIDPLRPEKMKLAFSKKIFGDNFLTDM
                     AYDRNGNIIVSTWVNGLFIIDSSYTIISHLKRDKNNINSIGSNSINTLYVDRTNTLWI
                     GFTDNGVSMLQLDKKMFTTLDVNSPISNDLNIYSIVRDRRNNLWIGSRGQGLIKYNLI
                     NKTFQTYLVGQQKGFVTNSYLSFKVGLDNKIWIGTDGGFLYRYDEIDGSFAHVKNRID
                     DWSSAVFCIAEDEHFVWCGTWGGGIKKVDKKTLEYVSINFDDKDQHRNSVFDIEIHDS
                     SLW"
BASE COUNT          862 a          481 c          625 g          770 t
ORIGIN      
        1 attgtacaaa gttgaaaaaa atctttcatg tagtgtaact ttttgggaaa ggcttccgtc
       61 atacgagcaa aaaggaatca attaaaaaag agatatcatg aaaacacaac ttttatcgtt
      121 aattttaatt tgtatatcga atgttgtatt tgcacagaat cagcccctta gccaacaggc
      181 tgattctgta gtgagcaatg cagaccaatt gccattggat gagcttcttt cggaaaatga
      241 tagtttggca gaagcagcag tactaaagcc cgattcggta aacaaaggtt caggcactga
      301 aaagatcgat acggttcata ttcgcgttgg aaaacatgcg attgagattg ttaccgacga
      361 cgatcgtacc caaattgatg ttgataagct tgaagagttt ggtaaacata tcgagcaatc
      421 ggaaattgag agggctgagc gaaaagctga aaagcataaa aagaaaaagt tcaacggaca
      481 ttggggaggc attgattttg gtggaaacca actactaaac accacttatc ccgaatctct
      541 gtatccggct ggaaccccgg aatttatgat tacggcacct gaaaaatcgt tcgaagtgaa
      601 tgtcaatctc tttgaataca gttttggatt ttcttcatac gtagggttgg ttacaggctt
      661 agggcttaat ttcaacgatt ataaatttag gaaccggtat accctggtaa aagatgataa
      721 tggagtaatt caaccgcaat cgttgcccga gggtgatttt aggctgagca agctttctac
      781 aacattcatt catgctccgt taatgcttga ggtgcaaata cctggtcaat acgatcataa
      841 acgaattttt attgccgggg gtgttatcgg gggcgtgaaa attcatgagc atgtaaaaac
      901 caagattggc agcgaaaaga aacgcgataa tgggaactac aacgttgctc ctattcgttg
      961 gggatacaca gcacgagttg gtttcgagga tgttggaata tttgcaacat actataatac
     1021 aaagtttttt gagaaagggt taggccccga ggccactcct tttaccgtgg gacttgcttt
     1081 tgctttctaa ccataaaaat ataattaggt ttaagaggtc gtcgaaaagg gcggcctctt
     1141 ttggttttct atccaataat ttgattaaat tggtacggta aaaaacaggg atagaaaaca
     1201 gggtaatggg aagaatcgtt ccattttttt ggttggtatt ttttgtaata ccggccactt
     1261 ttggttcccc gtccgatttt gttaatttca ataccggaaa cggaaaactc gggcacaata
     1321 ctgttgaagc agttgtgcgc gatacctttg gctatttgtg ggtgggtact aactatgggt
     1381 taaaccgcct ggatggctat caaacaatca acttcctctc cgacccggaa gactcgagtt
     1441 ccatttcaag caattttatt ctcagccttt ttgtcgatac tgaaaacaat gtctgggtgg
     1501 gaaccctggg cggagggcta aacatgtatg accgaaatac cgggaggttc aagcggtttg
     1561 taaccaccga taagcccgga ggaattggaa gcctaaacat ttctgaaata atcgaagatt
     1621 taaacggaga tttatggatt ggaaccaacg ggaacggggt taaccggttc aataaaaaaa
     1681 caggggtgtt ttcacactat aacatagagc aatatgatcc cgaacatcgg aaaaactcga
     1741 atgttacaaa gctttttcga gattgtgaag gaaacatttg ggtgggtatg aaccaaagcg
     1801 aggtctttaa aattgatcca aagacagaaa aaattacttt tcatggtttg acaagatccg
     1861 acaacggttt tcgtgatatt ggggcaatat taggatttat acaagaaggt agcgggagat
     1921 tgcttatctc ggggcgcagc gggaacatct accacatcga cccattgcgc cctgaaaaaa
     1981 tgaagcttgc ttttagcaaa aagatttttg gagataattt tcttaccgac atggcttacg
     2041 atcgaaacgg caacataatt gttagcacat gggtaaatgg cctttttatt attgacagct
     2101 catataccat aattagtcat ttaaagcgag ataaaaacaa tattaatagc atagggtcaa
     2161 attctataaa tacattatat gtcgaccgga ccaatacgtt atggattggt tttaccgata
     2221 acggggtcag tatgttgcaa ttagacaaga agatgttcac cactcttgat gttaattccc
     2281 caatatcgaa cgatttaaat atctatagta tagttcgcga ccgcaggaac aacttgtgga
     2341 ttggcagccg ggggcaaggg cttattaagt ataacctgat taataaaaca tttcaaacct
     2401 atctagttgg acagcaaaaa ggctttgtta ccaattcgta cctttcgttt aaagttggtc
     2461 tcgacaataa gatttggatt ggaaccgatg gtgggtttct ataccgctac gacgaaatcg
     2521 atggtagttt tgcccatgta aaaaaccgaa tcgacgactg gagtagtgcc gtattttgca
     2581 ttgctgaaga tgaacatttt gtttggtgcg gaacttgggg aggtggcata aaaaaagtag
     2641 ataagaaaac gctggaatat gtgtcgataa attttgacga caaagaccaa caccggaaca
     2701 gtgttttcga catcgaaata catgactcgt ccttgtgg
//
LOCUS       DAOPUW010000229         2737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_38564_length_2737_cov_3.916480, whole genome shotgun sequence.
ACCESSION   DAOPUW010000229 DAOPUW010000000
VERSION     DAOPUW010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38564_length_2737_cov_3.916480"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..493)
                     /locus_tag="PKJ43_04800"
     CDS             complement(41..493)
                     /locus_tag="PKJ43_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71917.1"
                     /translation="MENTKKQVLADLLSDLESKTFEIEAWKQKATLLIKRIFGDNDPK
                     IALVEELHYDFSSWSLRDSSGGKSNDKVKEAARGIIDAAIAEISLEGGDNVVVEVLRY
                     ELSGKDFETLNSIMASAQDREALLSGFIASVSNEVKDLIIKKLIEKSM"
     gene            complement(495..1289)
                     /locus_tag="PKJ43_04805"
     CDS             complement(495..1289)
                     /locus_tag="PKJ43_04805"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNZ71918.1"
                     /translation="MNYSELCEKVKVIVEDTAKYIQAERKNFSAQNDVERKGHSNFVT
                     YVDKGSEKRLISALGNLLPGSGFIAEEGTSDLKGEKYNWIIDPLDGTTNFIHGLPPHS
                     ISVALQEDNILVLGVVCEINSGEMFHAVRGGGAFLNNKAISVSVNATHQQALIATGFP
                     YSNFSKLDSYMFAMREIMEKTSGIRRLGSAAIDLCYVACGRFDAFWETHLNPWDVAAG
                     ALLVMEAGGRVSDFSGGENYLTGNQIIASNSLYYSDFYEIVNKYLG"
     gene            complement(1361..>2737)
                     /locus_tag="PKJ43_04810"
     CDS             complement(1361..>2737)
                     /locus_tag="PKJ43_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNZ71919.1"
                     /translation="NIYGVNGNTLEFAAEPYGPSWPNPPWSTLFLRKLLMNNDFKNQF
                     ITRFSDCLNSTFLVDNLSTKVDSIVRVISREIPSHLDKWNLSYENWKSNVSTVKSFIY
                     GRRADMREYIREYFGFDYDKLLTISLSEKNSGKIRANTIIPGSYPFQGYYFSEVPVTI
                     EALPNPGYRFVRWGGSVSSNERVITFSMNVNFSITAYFEPVNTIDEQVVINEINYKSP
                     NDFDSGDWIELYNNGSQTVDLSGWVLRDIDPEKSFVFSVGTYLYPGQYLVVASKASTF
                     RSVFPSIRNVIGDLSFGLSSSGETIWLYNNSGILIDQVRYDIRTPWSPLACGFGPSLE
                     LKSPSLDNALGNNWGTGDKGGTPGRVNAFTVDSRQLVQQKGFAVCYPTVFTHQTTLQF
                     FDENGGNYSVQVLDLQGRLYETITGQSTGTDTSVNLFKNSEKYTPGIYLIKLQTRGWT
                     ETLKVVKR"
BASE COUNT          841 a          626 c          470 g          800 t
ORIGIN      
        1 agtcctgttc taatcagtca acaataattt aagtaagccg ttacatcgat ttctcgatta
       61 actttttaat gattaaatct ttaacttcat ttgaaactga ggcaataaaa cccgacaaaa
      121 gagcttcgcg atcctgggct gaggccataa ttgagttcag cgtttcaaaa tctttccctg
      181 aaagttcata acgaagcacc tcaacaacaa cattatcacc accctcaagc gaaatttctg
      241 caatcgcagc atcgataatt ccgcgggcag cttcttttac tttatcatta gactttccac
      301 ctgagctgtc gcgcaacgac caacttgaaa aatcgtagtg taactcttca actaaagcaa
      361 ttttaggatc attgtctcca aaaatccgtt taatcaacaa cgttgctttc tgtttccatg
      421 cttcaatctc aaatgtttta ctttcgagat cgcttagcaa atcggctaaa acttgttttt
      481 ttgtattttc catgttatcc aaggtattta ttcacaattt cgtaaaaatc agaataataa
      541 agtgaatttg aagctataat ctggttcccg gtcaaataat tttctccacc agagaaatct
      601 gaaacacgcc cacctgcctc catcaccaat aaagcacctg cggcaacatc ccacgggtta
      661 aggtgagttt cccaaaaagc atcgaaacgc ccacaagcga cataacataa atcaattgca
      721 gccgagccca gacgacgtat cccgctggtt ttctccataa tttcacgcat tgcaaacata
      781 tagctgtcta attttgaaaa attgctatat gggaatccgg tagcgataag tgcctgttga
      841 tgagtagcgt taaccgacac agatatagcc ttgttgttta aaaaggcacc accgcccctg
      901 accgcatgaa acatctcgcc agaatttatt tcacacacta caccaagcac cagtatgttg
      961 tcctcctgaa gggcaacact tatcgaatgg gggggcaaac catggataaa gttcgtggtc
     1021 ccgtcgagcg gatcgataat ccaattatat ttttcgccct tcaggtctga ggtgccttct
     1081 tcggcaataa aacctgaacc cgggagcaaa ttacccaaag cagaaatcaa ccgtttctca
     1141 gatcccttat caacataagt tacgaagttg ctatgccctt ttcgttcaac atcgttttgt
     1201 gccgagaaat tctttctttc ggcttgaata tattttgcgg tgtcttctac aatcaccttt
     1261 actttttcac acaattcact ataattcatc tctttgcgtt aattaggaaa aactacaccc
     1321 aacaaacaag tgctttgcta aaaattgaag gacaaattta ttaccgtttt accaccttaa
     1381 gtgtttctgt ccagcctctg gtctgcaatt taatcaaata aattccaggt gtatatttct
     1441 cactgttctt gaacaaatta accgatgtat ctgtgccagt agactggcct gttattgttt
     1501 catacaatcg tccttgcaaa tctaagacct gcactgaata attccctcca ttttcatcaa
     1561 aaaattgcaa tgtcgtttgg tgtgtaaaaa ccgttggata acaaactgca aaacccttct
     1621 gttggacaag ttgtcttgaa tctacagtaa aggcattgac tcgtcccggg gttccccctt
     1681 tatcacctgt tccccaattg tttcccaagg cattgtcgag tgatggactt ttcagctcta
     1741 acgaaggtcc aaaaccgcag gcaagaggag accatggagt acgtatatcg taccgaacct
     1801 gatcgataag tataccggaa ttgttatata gccaaatggt ttcgccacta ctgcttagcc
     1861 caaacgacaa atctcctatt acattccgaa tactgggaaa aacagatctg aatgttgaag
     1921 cttttgatgc aacaacaaga tattgccccg gatacaaata ggttccaaca gaaaaaacaa
     1981 atgatttttc aggatcaata tcgcgaagta cccaacccga taagtcaaca gtctggctcc
     2041 cgttattata taattcgatc cagtctcccg aatcaaaatc gttgggcgat ttataattta
     2101 tttcattgat aaccacttgc tcatcaatag tatttacagg ttcaaaataa gcagtaatgc
     2161 taaaattaac gttcatcgaa aaagtaatta cccgttcatt cgagcttact gatcctcccc
     2221 atcggacaaa acggtagcca gggtttggca aagcctcaat tgtaacagga acttcactaa
     2281 aataatatcc ctggaatggg taacttcccg ggataatggt gtttgccctt atctttccac
     2341 tatttttttc ggaaagggag attgtcaata atttatcata gtcaaatcca aaatattccc
     2401 tgatatattc tcgcatatct gcacgccgcc cgtaaatgaa cgatttcact gttgaaacat
     2461 tcgatttcca attctcataa ctgagattcc atttgtcgag atgagacgga atttctctag
     2521 aaataacccg tacaattgaa tcaactttgg tactaaggtt atcgacgaga aaagtactgt
     2581 tcaaacaatc ggagaaacgc gttataaatt gatttttaaa atcattgttc atcagtaatt
     2641 tacgaaggaa aagagtagac cagggaggat tgggccacga cggtccataa ggctcagcag
     2701 caaactcgag cgtattcccg tttaccccat aaatatt
//
LOCUS       DAOPUW010000230         2732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_38673_length_2732_cov_3.465073, whole genome shotgun sequence.
ACCESSION   DAOPUW010000230 DAOPUW010000000
VERSION     DAOPUW010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38673_length_2732_cov_3.465073"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="PKJ43_04815"
     CDS             complement(<1..1226)
                     /locus_tag="PKJ43_04815"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ71920.1"
                     /translation="MKKSLLLYFLILSISLLGNPSFRVSGTVSDATSGEDLIGASVYV
                     KQIKHGTTTNTYGFYTMSMSPGLYSFSVSFLGYETLEYTLAVERDMVYNIKLNPRSEE
                     IGEIVVSTEARNQNIVSNEMGTVKLSPQAIKVIPVIFGEQDVLKTIQLLPGVSSSGEG
                     STGFFVRGGQADQNLVLLDDAQVFNSGHLLGFFSVFNSDVVNDVKLYKGGVPANFGGR
                     SSSVLDVRMREGNSEHFSASGGLGLISSRLTLEGPIVKNISSFLLSGRRTYADLFLLL
                     ADNEILRKSKLYFYDLNAKIHVDFGDKDRLFLSGYLGRDALKTDIMGFDWGNKVGTMR
                     WFHQFSPRFVSNTSLIYNDYDYKTSANLDFSFDLKAGVIGQNVKQRFIWYINNNNTVS
                     FGLEATRYVFKPGNLK"
     gene            complement(1318..2253)
                     /gene="queG"
                     /locus_tag="PKJ43_04820"
     CDS             complement(1318..2253)
                     /gene="queG"
                     /locus_tag="PKJ43_04820"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNZ71921.1"
                     /translation="MDIARRSELIRKKALSLGFSACGFANVEKLVEQEQGLRDYLENN
                     YYGEMTYMANHFDKRLDPSLLVPGAKSAIVVLLNYFPKKVLSNETGLMLSKYAYGEDY
                     HEVVKGKLAKLFDFIGAEIAPVQGRYFCDSAPVLERAWAVKAGLGWIGKNGLLINRKL
                     GSFVFIGELFVDIELEYDHSHEKKYCGSCTRCLNSCPSSAFVAPYRLDARKCISYLTI
                     ELKGEVPEKLKPALKNRIFGCDICQDVCPWNQKLSPHDVHEFAPKQELLEFTSSDWEN
                     ITHEGFTALFSKSALKRAGYSKLKQNIASVMSVDN"
     gene            complement(2279..>2732)
                     /locus_tag="PKJ43_04825"
     CDS             complement(2279..>2732)
                     /locus_tag="PKJ43_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HNZ71922.1"
                     /translation="WAGIAALGFGVLFNVPQRTLWAIAWIGALGSFVKFVLLEVDVNI
                     IIASLVGASLVGIVSIPLAHEIHSPPLIFSIPAVIPMVPGALAYQMMIGLMKIAVHTT
                     ETGMEVVLTDTFQSAMKVTFILLSLAVGVAVPMLVTRKESAKRIRIKI"
BASE COUNT          886 a          630 c          499 g          717 t
ORIGIN      
        1 atttttaggt tacctggctt aaaaacataa cgggttgcct caagtccaaa agaaacagta
       61 ttgttattat tgatatacca tataaagcgt tgtttcacat tctgcccaat aactccggcc
      121 ttaaggtcga atgaaaaatc gagatttgca gaagtcttat aatcataatc gttgtaaata
      181 agcgaagtat tgctaacaaa ccggggcgaa aattgatgaa accagcgcat ggtccccact
      241 ttattgcccc aatcgaaacc cataatatca gtttttaacg catcgcgacc gagatagcca
      301 gagagaaaaa gtcggtcttt atccccaaaa tcgacatgga tttttgcatt taagtcgtaa
      361 aaatatagtt tgcttttgcg cagtatttca ttatcggcca acaaaagaaa taaatcggca
      421 taggtgcggc ggcccgacaa aagaaacgaa cttatatttt taacaatagg cccttccagt
      481 gtcaacctac tggatattaa ccccaatcct ccagatgcag aaaagtgttc actgttgcct
      541 tcgcgcatac ggacatcgag tactgaagaa gatcgtccac caaaattagc aggaacaccg
      601 cctttgtata gctttacgtc gttcactaca tcagagttga aaaccgagaa aaaccctagc
      661 aaatgaccag aattgaaaac ctgagcatcg tcgagcaaaa caaggttctg atcagcctgc
      721 cccccacgaa caaaaaagcc agtgctccct tcgcctgagg aagacacccc cggaagtaac
      781 tgtatggttt tcaacacatc ttgctccccg aaaattaccg gaataacttt aatggcttgg
      841 ggcgataatt ttaccgtacc catttcatta cttacaatat tctgattgcg ggcctcggta
      901 ctgaccacta tttcaccaat ttcttcagac cttggattga gttttatgtt ataaaccata
      961 tcgcgctcaa cagccaaagt atattcaaga gtctcgtacc ctaaaaaact tacggaaaat
     1021 gaataaaggc cgggggacat actcatggta taaaacccat atgtattagt tgtagtccca
     1081 tgctttatct gctttacata caccgaggca ccaatcaaat cttcccccga tgtggcatcg
     1141 ctaacagtac cgctaaccct aaaagatgga tttcccaaaa gcgatattga taaaattaaa
     1201 aaatacaaca agagtgattt tttcatcgcg ttttcatcat taatgttaca actattgaca
     1261 gaaaaattac cccacagttg cttttgattt atcttatgaa agtccgattg tatccgacta
     1321 attgtcgaca gacatcacac tggcaatatt ttgcttcaac tttgaatacc ctgcacgttt
     1381 taatgctgat ttagaaaaca aagcagtaaa accttcatgt gtaatatttt cccaatcaga
     1441 ggatgtgaat tcaagcaatt cttgtttagg agcaaactcg tgaacatcat gaggagagag
     1501 cttttgattc caaggacaaa catcctgaca aatatcacaa ccaaaaatcc tgttttttaa
     1561 tgctggtttt aatttttccg ggacttcccc cttaagctca attgttagat aagaaataca
     1621 ctttcgggca tcaagccgat aaggagcaac aaaggctgaa gaggggcacg agtttaaaca
     1681 acgggtacaa ctcccacaat attttttttc gtgtgagtga tcgtactcaa gctcaatatc
     1741 gacaaaaagt tcaccaatga aaacaaacga acccaacttg cggttaatca gtagtccatt
     1801 ttttccaatc caacctaacc ccgctttcac agcccaagcc cgttccaaaa caggtgcaga
     1861 atcacaaaaa tacctgcctt gaacaggagc aatttccgcc ccaataaaat cgaaaagctt
     1921 ggctaattta cccttaacaa cctcgtgata gtcttcccca taggcatatt ttgaaagcat
     1981 aaggcctgtt tcattggaaa gtactttttt ggggaaataa ttcaaaagaa caacaattgc
     2041 cgattttgcc ccgggaacta aaagcgaagg atcgaggcgc ttgtcgaaat gattagccat
     2101 gtaggtcatc tcgccgtaat aattgttttc aagataatct cttaagccct gttcttgctc
     2161 aaccagcttc tcaacattgg caaagccgca agccgaaaaa cccaacgaaa gggctttctt
     2221 cctaattaat tcagagcgac gggcaatatc catttcaatg ttcttaataa acctgaacct
     2281 atatttttat gcgtatcctt tttgccgact cttttcgggt aaccaacatc ggtacagcta
     2341 caccaacagc gagggaaagg agaatgaagg taactttcat ggccgactga aaggtatcgg
     2401 taagtacaac ttccattcct gtctcggtag tatgaactgc aattttcatt aacccgatca
     2461 tcatttggta ggccaaagca cccggcacca tagggataac cgccggaata gaaaaaataa
     2521 ggggtggaga atgaatctcg tgagccagtg gtatgcttac tataccgacg agagatgcgc
     2581 ccacaagtga agcaataatt atattgacat caacctccag gagaacaaac ttcacaaaac
     2641 ttccgagagc cccaatccag gcaattgccc aaagtgtcct ttgaggcacg ttgaataaaa
     2701 ctccaaaacc aagagcagct attcccgccc aa
//
LOCUS       DAOPUW010000231         2729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_38724_length_2729_cov_3.600224, whole genome shotgun sequence.
ACCESSION   DAOPUW010000231 DAOPUW010000000
VERSION     DAOPUW010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38724_length_2729_cov_3.600224"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..851)
                     /locus_tag="PKJ43_04830"
     CDS             complement(210..851)
                     /locus_tag="PKJ43_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71923.1"
                     /translation="MRNLILLAAFLIGSIISGFAANYKETMIQTIEKLNKSQDANELV
                     EIANTFDRIAQKESTEWLPLYYSAYATISTTFFNQAMSNEEKVKVFEKAQQKLDQAKK
                     LADNEDEIYVLQAMIYQLKISTDNNNAYQYIMKANEALGRAESIDAKNPRIPYLKGTT
                     LFYTPTEYGGGASVAKPLFEKAAKLFSEADLSNQLMPNWGAYHNNMMLEQCNK"
     gene            complement(955..>2729)
                     /locus_tag="PKJ43_04835"
     CDS             complement(955..>2729)
                     /locus_tag="PKJ43_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ71924.1"
                     /translation="PLDIYTTAGSNGDIMAAMKTMPGSQSSPDDGRLMVRGGDAYESK
                     TYIDGFLAAQPYYSKTPDVATRGRFSPSLFSGVQFNSGGYSAEYGQALSSVLNLNSTD
                     LATTDVTGISIMTIGAEANITKAFKNNSIMASGSYMNMWPYHKLFPSNVNWTKPVESF
                     GVNLAHRYKPSSSAIWKNYFYADFGNMGYSSKESDNTLFTLNNNNQSLYFNTSYKDCF
                     SEESCYKFGLSSTYEANNTELNGLPIDTKEISADGRFNYYLQVGEAININAGINETFS
                     QYNQDVYAADKSKTWKSNLTDHTMGIFVESEIKFSKKIAIKPGLRGEYGTLLKQASIS
                     PRFALAVKTGKGSQLSGAWGIYYQNPEFDYLKFNSKLNLEKATHYILGFQSGNLEERL
                     FRTEAYYKTYNNLITWEGSNKYYPQHIANNGNGYARGIDVFWRDRKSIKYFEYWLTYS
                     FIDTKRKYQNYPEMATPDFVSAHNLAIVSKYWIHAITTQVGVSFNVASGRNYDNPATP
                     EFMDVKTGWYNSLNFNLSKVFFLGNQYSVLYFSVSNVLGSDPIVAYRPTSAADSNGNF
                     QLTPVKQDSKRFFFIGLFLNLDGK"
BASE COUNT          728 a          498 c          624 g          879 t
ORIGIN      
        1 gccaatagcc actgaaataa atgcagcaag aaataattgt ctgattaatc gtttcctatt
       61 tataaccctg tttttcatat tccgaggtaa agatacaaat tgaatttaag tcccggtaaa
      121 gctccaaaag acaaagattg agaatagcac cgggaaaatg ggtgaagaga atcttccatt
      181 ttcccggcga tcaatttatg ctcaaatttt tatttgttgc attgttccaa catcatgttg
      241 ttgtgataag ctccccagtt gggcatcaat tggtttgaaa gatcggcttc gctaaataac
      301 ttagcagctt tttcaaatag cggtttggcc acagaagctc ctccgccata ctcagtagga
      361 gtgtaaaaca atgtggttcc ttttagataa ggaatacggg gatttttagc atcgatggat
      421 tcggcacggc caagtgcttc atttgctttc ataatgtact gataagcatt gttattatct
      481 gtcgagatct taagctgata aatcatggct tgaagcacat aaatttcatc ttcattatcg
      541 gccaattttt ttgcctgatc gagtttttgc tgtgcttttt caaacacctt gaccttttct
      601 tcgtttgaca tggcttgatt gaagaatgtt gtactaatgg ttgcgtaggc agaatagtaa
      661 agtggaagcc attcggtgga ttctttttgg gcaattcggt cgaaagtgtt tgcaatctcg
      721 acaagctcat tggcatcttg cgatttgttt agtttttcaa tggtttggat cattgtttcc
      781 ttatagttgg ctgcgaagcc cgaaataatt gatccgatta aaaatgcggc aagtaagatt
      841 agatttctca tggcaaaaat ttattaattg tttatgcttc aaaattgttt tgtttatact
      901 gtatgtgaaa ttttattgtc ctgaattgtg gaaaatggtt actgaattgt cgctttattt
      961 cccatcgagg ttaaggaaca gcccgatgaa aaagaatcgc ttcgagtctt gttttactgg
     1021 ggttaactga aaattgccgt tactgtcggc tgccgatgta ggacgatagg ccacaatggg
     1081 gtcgctgccc aacacattgg atacagagaa atagagcaca gaatattgat ttcccaggaa
     1141 aaatacttta ctcaggttaa aattcaagct attgtaccat cctgttttaa catccatgaa
     1201 ctcaggagtt gccgggttgt cgtaattacg gcccgaagca acattgaacg ataccccaac
     1261 ttgcgtggtg atggcatgaa tccagtattt tgaaacgata gcaaggttgt gggccgaaac
     1321 aaaatcgggg gtggccattt ctggatagtt ttggtatttc cgttttgtat cgatgaacga
     1381 ataagtcagc caatattcga agtactttat gcttttgcga tcgcgccaga aaacatcaat
     1441 accacgggca tacccatttc cattgttggc aatgtgttga gggtaatatt tgttgctgcc
     1501 ctcccaggtg attaggttgt tataggtttt atagtaagcc tctgtacgga aaaggcgttc
     1561 ttccaaatta cccgattgga atccaagaat gtagtgtgtc gctttttcca gatttagttt
     1621 tgagttgaac ttcaggtaat cgaattcagg attttgataa taaatacccc atgcacctga
     1681 gagctggctc ccttttcctg tttttactgc gagggcaaag cgtggtgaaa tggaagcttg
     1741 tttaagtagt gtaccatatt cgccccttaa tccgggttta atggcaattt ttttactgaa
     1801 tttaatttca ctttcgacga aaatgcccat ggtatggtcg gtcaaattac ttttccaggt
     1861 tttactttta tcggcagcat aaacatcctg attgtattgg ctaaaagttt cgttaatacc
     1921 agcatttatg tttatggctt cgccaacctg aagataatag ttgaaacgtc catcggctga
     1981 aatttctttt gtgtcgatgg gcaatccatt aagctctgta ttattagctt cgtaagtgga
     2041 tgaaagtcca aatttatagc agctttcttc cgagaaacaa tctttatagc tggtgttgaa
     2101 atagagcgat tggttgttgt tgttcaatgt gaaaagggtg ttgtcgcttt cttttgatga
     2161 gtatcccata tttccgaaat cggcatagaa gtagtttttc cagattgcac ttgacgaagg
     2221 tttgtagcgg tgtgcaaggt ttacgccaaa tgattcaacg ggtttggtcc agtttacatt
     2281 tgaagggaaa agtttgtggt agggccacat gttcatgtac gagcccgatg ccataattga
     2341 gttattcttg aatgctttgg taatatttgc ttcggcaccg atggtcatga ttgaaatccc
     2401 ggttacatcg gtggttgcca agtcggtcga gttgaggttt aataccgaag agagagcttg
     2461 cccgtattca gcggaatagc ctcctgaatt gaattgaacg ccgctgaaaa gtgaaggtga
     2521 aaatcgtccg cgagtagcca catcgggagt tttggagtaa taaggttggg ccgcaagaaa
     2581 accgtcgatg taggtttttg attcgtaagc atcgcctcca cgtaccatca aacgaccgtc
     2641 atcgggcgac gattgagagc cgggcattgt tttcatggca gccatgatat cgccgttcga
     2701 tcccgcagtg gtgtaaatgt cgagaggct
//
LOCUS       DAOPUW010000232         2728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_38752_length_2728_cov_2.339693, whole genome shotgun sequence.
ACCESSION   DAOPUW010000232 DAOPUW010000000
VERSION     DAOPUW010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38752_length_2728_cov_2.339693"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(468..1628)
                     /locus_tag="PKJ43_04840"
     CDS             complement(468..1628)
                     /locus_tag="PKJ43_04840"
                     /inference="COORDINATES: protein motif:HMM:NF033538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HNZ71925.1"
                     /translation="MENIRQQFDIADIFSNYKNIVENNEKLCHEQIKAFNDIVACRTD
                     ALGGHKAVCDHCGNLSISYNSCRNRHCPKCQFTKKALWVDKIAAKLPPVKHFHLVFTV
                     PHSLNKLFYLNQAAAYNCLFKAAGKALIKLTANPDFLGAQTGAVAILHTWGQNLSYHP
                     HVHMIVPAGGLSEDATEWVPANPKFLVPVKVLGRIFRGEMYRSMLNALQSETMKLPDD
                     IADYNDFKARCYLKNWVVYCERPFCNAESLIAYLGNYTHRVAISNHRIQNFSNGKVRL
                     SCKDYRNAGKKRSIELDAKEFIRRFLQHILPKGFYKIRYFGFMAMRNAKTKLAQCFEL
                     IDKTGWLPILQGLPALDIFRLITGNDPIVCPICKTGKLKYLINQCNPIAAPG"
     gene            complement(1643..2491)
                     /locus_tag="PKJ43_04845"
     CDS             complement(1643..2491)
                     /locus_tag="PKJ43_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNZ71926.1"
                     /translation="MLEKSNNINQLVFEMQYRNYSSRTIKTYQELLRHLEVRLSTSLA
                     DITTEQLKHYLHKRIVDEEISVSTINQTISAFKIFQTDVLKKDWETFKIKRPRREKKL
                     PVVLSEQEIEMLIGATRNLKHRAILTLAYSAGLRLQEVQTMKPNAIDSKRMMVRVVQG
                     KGKKDRYTLLSQKALLLLRDYYKLYRPKNYLFETQCNAGQFLAETTFNNIIKNNAAKA
                     GINKQVSFHVLRHSFATHLLEHGVNLKAIQQLMGHNSLKTTSIYLHIANLNPAAIVSP
                     LEMMNF"
BASE COUNT          724 a          531 c          561 g          912 t
ORIGIN      
        1 gaagggacgg ttgcagggaa gggcagccac actcttgctt ttgtgggctg ggtttccatt
       61 ttcttctttg tctttatgca gcaaaaattt cattgtcatt gtgtcgttgt ttttttcttc
      121 ggcttgttgc caacttacca gcattttatt catcgttttt cacaaaatta aaacaaatag
      181 ctgcaaacag caatttttgg attaaaattt tgcatgatgg acaaggaaaa tgatgaataa
      241 aatgaagttg gactaagtcg ctgtttaggt tatgggcatt ttattttatg tagccctgtg
      301 cgaagccaaa aaaattgcat tcgattccgg tttattgtat tttgattgat tgtttggccc
      361 cggattccgt aacaaccaat ggaaaaagtg catagcattg ccattatgat ttggtcattt
      421 caatttctaa tctgtaattt tcaaagaaca aagtaatagg atcaagctca acctggtgct
      481 gcaataggat tgcattgatt aattaggtat tttagttttc ctgttttgca tattgggcaa
      541 acaattggat cgttaccggt aattagcctg aagatgtcta acgccggcag tccttgtaat
      601 atcggcaacc agcctgtttt atcaataagt tcaaagcatt gtgcgagttt tgtttttgca
      661 tttcgcatag ccataaaccc aaaatatctg attttgtaaa aacctttcgg caaaatgtgt
      721 tgcaggaatc tcctgataaa ctctttagca tccagttcga tactgcgctt tttccccgca
      781 tttcgataat ctttgcatga taagcgcacc ttcccattac taaagttttg tatgcgatga
      841 ttggatatag ccacgcgatg ggtatagttt cccagatagg ctataagcga ttcggcattg
      901 caaaagggcc gctcgcagta gaccacccag tttttaagat agcatctcgc tttaaaatcg
      961 ttataatcgg cgatgtcatc gggtaacttc attgtttctg attgcaatgc attgagcata
     1021 ctgcggtaca tttcacctct aaaaattcgc ccgagcactt ttacaggaac caaaaacttt
     1081 gggtttgccg gaacccattc tgtagcatct tccgacaagc caccggcagg aacaatcatg
     1141 tgaacatgtg ggtggtaact cagattttgc ccccaagtat gtaatatggc aaccgccccg
     1201 gtttgtgctc ccagaaaatc ggggtttgca gtcagcttta tcaatgcctt tccggcagct
     1261 ttgaacagac aattatatgc cgcagcctga ttcaagtaga acaacttatt gagcgagtgt
     1321 ggcaccgtaa aaaccagatg aaagtgcttc acaggaggca gcttggcagc tatcttatcg
     1381 acccatagtg cttttttagt gaactgacac ttagggcagt gtcggtttct gcacgagttg
     1441 tacgatatgc tcaggtttcc gcaatggtcg caaacggctt tgtgtccacc caaagcatcg
     1501 gttctgcatg caacaatgtc gttaaaggct tttatttgtt cgtggcacag tttttcattg
     1561 ttttccacga tgttcttgta attgctgaaa atatcagcta tatcaaattg ttgacgtatg
     1621 ttttccatga atagagtgcg ttttagaagt tcatcatctc taatggcgaa acaatggcag
     1681 cagggtttaa attggcaatg tgcaaatata tcgaagtggt tttaagcgaa ttgtgcccca
     1741 tgagttgctg aatggctttt aaattaacac catgttcgag cagatgagtc gcaaaactat
     1801 gtcgcagcac atgaaaagaa acctgcttgt taataccggc tttggcggca ttattcttta
     1861 ttatattgtt gaaggtagtt tctgccaaaa actgccctgc attacactga gtttcaaaca
     1921 aatagttttt tgggcgatac aatttgtaat agtctctaag caacaataga gctttctgag
     1981 agagtagtgt ataacggtct tttttccctt ttccttgcac cacacgcacc atcattcttt
     2041 tcgagtcaat agcgtttggt ttcatggttt gtacttcttg caggcgtaat ccggcagagt
     2101 aggcaagggt gagtattgcc ctatgtttta agttgcgcgt tgccccgatt aacatctcta
     2161 tttcctgttc cgacagcaca accggaagtt tcttctcacg ccgtggtcgc tttattttaa
     2221 aagtttccca atcttttttt aatacatcgg tttggaaaat tttaaaagca ctgatggttt
     2281 gatttatcgt tgaaacagaa atttcctcat cgacaatgcg cttgtgcaaa tagtgcttta
     2341 actgctctgt ggtaatatct gcaagtgatg tagaaagcct gacttcaagg tgtctcagta
     2401 gttcttgata cgtctttatg gttcggctgc tgtaattgcg atactgcatc tcgaaaacca
     2461 attgattgat gttgttcgat ttttctagca taacggtaaa ttttttggtt aataaccctc
     2521 aaatttaccg attcattagc gccactatgg tggcgattta gttcaactcg tttataacct
     2581 taccaaaagt aagcttacct attaaaattt atcgtataag cttaccattg cttcggtttt
     2641 attgcttcaa agctacaaaa cattttttga aatattcata ggcgtttcgt gttttttata
     2701 gcagtgtgtt ttactgaatt atgtggag
//
LOCUS       DAOPUW010000233         2726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_38783_length_2726_cov_3.877200, whole genome shotgun sequence.
ACCESSION   DAOPUW010000233 DAOPUW010000000
VERSION     DAOPUW010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38783_length_2726_cov_3.877200"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="PKJ43_04850"
     CDS             complement(<1..1026)
                     /locus_tag="PKJ43_04850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNZ71927.1"
                     /translation="MKENRLLNDLFCRKIFKAVALLIMLCLLIPGFSSANALQNKKTV
                     KGVVTDQKTSAPLIGVTVVVKGTTVGSITDIDGNYSLAASDKDVLEFSFIGYLRKEVR
                     VADQTTINVSLAEDIVGLDEVVVTGYGVQKKSDLTGAVSSVSGEKISSIPVVSVEHAL
                     QGQAAGVNIIPKSGRPGEGADIQIRGISSINGTSPLVIIDGVQGDLNKLNPGDIASIE
                     VLKDASSAAIYGATGGNGVILVSTKNGKAGKMKVNFNIYRGFDNPVGKVEMMNSQEWM
                     GVLEEIDYEGKKSKLKEGEFVKAINARPDTLKTYDWQDIVFQQAQTENYDLSISGGNE
                     SSVFLFST"
     gene            complement(1632..2576)
                     /locus_tag="PKJ43_04855"
     CDS             complement(1632..2576)
                     /locus_tag="PKJ43_04855"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNZ71928.1"
                     /translation="MDVQELSRQEYTARINRVMDYISQNLDQPIDLSSMAEVASFSPY
                     HFHRIFTFIVKETPNNFVSRIKLEKAAQMLLDNHKASVSDIAAHCGFVNISSFSRAFK
                     LFFGLSAMEFRRKGKAIFIKNGIRYSKNGKMLSKIGQKNEGENNQFCSVELNQLIIMD
                     TKIEVKQMPEFNLIYCRHMGAFDQIGQAYEKLFKWAIPRGLVSNETKTATVYHDDPAI
                     TSVEKVRQDASIIVDGDVKVEGEIGKSTLRGGKYAVGRFELKETEFEEAWNTMCSWLT
                     ESGYQPGDGCTYELYHNSYSNHPDHRYIVDICIPVKPL"
BASE COUNT          711 a          548 c          549 g          918 t
ORIGIN      
        1 tgtgctgaat aagaaaacag acgattcatt accacccgaa atagagaggt catagttttc
       61 tgtttgtgct tgttggaaca ctatgtcctg ccaatcatag gttttcaatg tatcggggcg
      121 ggcgttaatt gcttttacaa attcgccttc tttgagttta ctttttttgc cttcgtaatc
      181 tatttcttca agaacgccca tccattcttg tgagttcatc atttcaactt ttcctacagg
      241 attgtcgaag ccgcggtata tattaaagtt tactttcatc tttccggctt tcccattttt
      301 tgttgaaaca agaataaccc cgttcccccc tgtagcacca tagattgcag cagaagaagc
      361 gtctttcaat acttcaattg aagcaatatc gccaggattt aatttgttta aatcaccttg
      421 tacaccgtcg atgataacca gaggactggt accgttgatt gagctgatcc cacggatctg
      481 gatatcggca ccttcacccg ggcgacccga tttagggata atgtttaccc cagcggcttg
      541 tccttgtaag gcatgttcaa cacttacaac tggaatactg ctaatttttt ctccagacac
      601 tgaagaaact gctccggtaa ggtcactttt tttctgaacc ccgtacccgg taaccactac
      661 ttcgtcgaga ccgacaatgt cttctgccaa cgatacgtta atggttgttt gatcggcaac
      721 acgtacttct tttctgaggt agccgataaa actgaattcc aaaacatctt tgtcagaagc
      781 tgccaaagaa tagttgccat cgatatcggt aattgaaccg accgttgtac ctttcacaac
      841 caccgttacc ccgataagtg gagccgatgt tttctggtca gtgacaaccc ctttaactgt
      901 ttttttgttt tgaagtgcat tggcactaga aaagccggga atcaaaaggc agagcattat
      961 taaaagtgct accgctttga aaatcttccg gcaaaacaaa tcgtttaaca atcgattttc
     1021 tttcatagat ttaaaggttt aatacatttg tttaaataaa gcattagctt cttgaatttt
     1081 cttttatttt aacactgcag ttaagcactt gttaaattat gcgccaaaga tattaaaata
     1141 taatttcaag aagcggtaaa agtagaaggc attcaacatt tgcattttta attttagaca
     1201 ttgatttgtt aattttagac aatttgggag agggattgtc cagcagatct gttatttcac
     1261 attgcccgtt ttattcgata ttttcatgtt tgctaaaagt tatagtgcat tttatgttgc
     1321 tgttttatgg gtcaaaaaaa tgaattttta tctgattctt tggaataata ccgattgtgt
     1381 gaattaaaat tgagtgctga tcagctttaa aataatgctc cgagatgcta atatttggga
     1441 aggctggtta gagtattttg catctatttt aggttttcct aaaggaaccg aaaatctatt
     1501 ttcaaatttt cctacatttt ttcacataca aagaataatg aaaaaccggc cccaacattt
     1561 tcctgttctt tttcgcgttt tgctattcag aaagtcgatt ggagaggccg ggtttgtttg
     1621 cttgtgattg cttagagcgg ttttaccgga atgcagatat caacaatata ccggtgatcg
     1681 gggtggttgc tgtagctgtt gtggtacagc tcgtaggtgc aaccgtcgcc tggctgatag
     1741 ccactttcgg taagccacga gcacatggtg ttccaggctt cttcgaattc ggtttccttt
     1801 agctcgaacc tgcctactgc atattttcct ccgcgtaggg tcgatttccc gatttcgcct
     1861 tcaactttaa catcgccatc gacaatgata ctggcatctt gccgtacttt ttccacactg
     1921 gtaatggccg ggtcgtcgtg ataaaccgtt gcggtttttg tttcgttgct gaccagtccg
     1981 cgtggaattg cccatttaaa caatttctcg tacgcttgcc caatttgatc gaaagcgccc
     2041 atgtggcgac aataaatcag gttgaattcg ggcatttgtt ttacttcaat ttttgtgtcc
     2101 ataataatta attgattgag ttcgacactg caaaattgat tgttttcgcc ttcgtttttt
     2161 tgcccgatct tgctgagcat tttgccattc ttgctatacc ttatcccgtt tttaataaaa
     2221 attgcttttc ctttccgacg gaactccatg gcacttaaac caaagaaaag tttgaaagcc
     2281 ctgctgaatg acgatatgtt tacaaaaccg cagtgggccg cgatgtcgct gactgatgcc
     2341 ttgtggttgt cgagcaacat ttgtgctgcc ttttcaagtt ttatcctcga tacaaaattg
     2401 tttggggttt cttttactat gaatgtgaat atacggtgaa aatgataggg cgagaacgaa
     2461 gccacttcgg ccattgatga taagtcgatc ggctggtcga ggttttggct tatataatcc
     2521 attacacgat tgatgcgtgc agtatactct tgtcggctta attcctgaac atccatggtt
     2581 ggttgaaaaa tatttttggc aaatatactg aatgagcagg tacattactt tgcaaatctt
     2641 gctgacaggg tggggcagaa gtgttctgtg tatttgtttc ccaacataac ccattttaaa
     2701 ggtttgcagg ttgtaaaaaa gtaaag
//
LOCUS       DAOPUW010000234         2726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_38786_length_2726_cov_3.146013, whole genome shotgun sequence.
ACCESSION   DAOPUW010000234 DAOPUW010000000
VERSION     DAOPUW010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38786_length_2726_cov_3.146013"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PKJ43_04860"
     CDS             complement(<1..564)
                     /locus_tag="PKJ43_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71929.1"
                     /translation="MDYLNKHKEGVIVTIIFHLILIFLLIRFGFFTPYPLPAEKGVLI
                     DFGTSENGQGMNEPTPKEATKTVTEPRPQTPPPATPKTTQPVAGKEEVVTQDLEKTAA
                     LEEIKKQEDLKRQKQMAEDKRRRDSLQRINEERIAELNRLAEIRRQDSLRKAEENARI
                     AQINSRAKNVFGSSGQGTDASAAGEGVT"
     gene            complement(548..982)
                     /locus_tag="PKJ43_04865"
     CDS             complement(548..982)
                     /locus_tag="PKJ43_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNZ71930.1"
                     /translation="MAIRRRSKIDSAMNMSSMSDLVFLLLIFFMITSTLVAPNALKLL
                     LPESNNQTQAKPISSISITQDLRYYVNDNGKLVQINFQDIEPFLLRTTRPDDETYISL
                     HADKSVPIEEVVKVMNIAQRNKFKMILATSPEQNSAKGGLFK"
     gene            complement(992..1678)
                     /locus_tag="PKJ43_04870"
     CDS             complement(992..1678)
                     /locus_tag="PKJ43_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNZ71931.1"
                     /translation="MLDFVLPQVNQTIGVLAVNQAGVTMNYGELALKGGWIMIPILAL
                     SLIAVFIFVDRFLAIRKASKVDPNLMNQIRQNVSEGKIDAALAICRSANHPITRMLEK
                     GIVRIGRPLEDVNKAIENVGNLEVAQLEKGLPWLATTAGGAPMLGFLGTVTGMVRAFF
                     DMASAGNNIDIQLLSSGIYEAMVTTVAGLIVGVMAYFGYNFLVSRIENLIFKMEMNTT
                     EFMDMLNEPA"
     gene            complement(1780..>2726)
                     /locus_tag="PKJ43_04875"
     CDS             complement(1780..>2726)
                     /locus_tag="PKJ43_04875"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNZ71932.1"
                     /translation="KVDKDFNIKLYGDYVIEKGDYLFTLENIINKRFTINQGGTIKWT
                     GEPYDADINLTAVYKVKTSLYDLFPESSTDMDLVRRMPVDCVISLSESLMQPKINFKI
                     ELPTAEQSIQDQVKQIIVTEEDVNKQMLSLLMLGRFYTPEIYAGKTTTVAGSEIVTNT
                     ASTTASELLSNQLSNWLSQISDQVDIGVNYRPGVTDGVKEISNDQIELALSTQVFNDR
                     VTIDGNIGNNSNPSANNSGEFIGEFDIKVKLTNDGRLQLKVYNHSNDGILNEEEPYTQ
                     GIGLSYREEFNTLKDLFRKYRNAILKKKKKKTGPKNEN"
BASE COUNT          693 a          548 c          552 g          933 t
ORIGIN      
        1 tgtaactcct tcgccagcag ccgatgcatc ggttccttga cctgaacttc caaaaacatt
       61 cttagctcgt gagtttattt gcgctatacg agcattttct tctgcttttc gcaaactatc
      121 ttgccgacga atttctgcta aacggttaag ttcagcaatg cgctcttcgt taatacgttg
      181 caaactatcc cttcggcgct tgtcttcagc catttgcttt tggcgtttga ggtcttcttg
      241 ttttttaatt tcttcgagcg cagcagtttt ttcaagatct tgtgtaacaa cctcttcttt
      301 tccagctact ggttgtgtgg ttttaggtgt tgctggtggt ggtgtttgtg gccggggttc
      361 agtaacggtt tttgtggctt ctttaggtgt aggttcgttc atcccctgac cattttcaga
      421 agtgccaaaa tcgattaaca cccctttctc cgctggtaac ggataaggag taaagaaccc
      481 aaacctgatg agtaaaaaaa tcaggatgag gtgaaaaata atggttacaa ttaccccttc
      541 tttatgttta tttaaataat ccaccctttg ctgaattttg ttccggactt gtagccaata
      601 tcattttaaa cttgttgcgc tgtgcaatat tcatcacttt aacaacttcc tctattggaa
      661 ccgatttgtc ggcatgcaac gagatgtatg tttcgtcatc ggggcgggtt gttcgtaata
      721 ggaatggctc aatatcctga aaattaatct gaaccaattt cccgttgtcg tttacgtagt
      781 aacgcaagtc ttgcgttatt gaaatcgatg agattggttt agcctgagtt tggttattgc
      841 tttcggggag caatagcttt agtgcattgg gtgctaccaa tgttgatgta atcatgaaaa
      901 agatgagaag caggaacacc aagtcactca tcgaagacat gttcatcgct gaatcgattt
      961 tacttcttcg tcttatggcc atggcaacta attatgcagg ttcattcaac atgtccataa
     1021 attcggtagt gttcatttcc attttgaata ttaaattttc aattctggaa acgagaaagt
     1081 tgtaaccgaa ataagccata acaccaacaa taagaccggc aacagtggta accatagctt
     1141 cataaatacc cgatgacaat agttggatat cgatgttgtt accggcactg gccatatcga
     1201 agaaagccct caccataccg gttacagttc ctaagaatcc aagcatagga gcgcccccgg
     1261 cagtagttgc cagccatggt aagccctttt cgagttgggc aacttcgagg ttgccaacat
     1321 tctcaatggc tttgttaacg tcttctagcg gcctgccaat acgtacaatt cctttttcga
     1381 gcatacgtgt aattgggtgg ttggccgaac ggcaaatggc taaggctgcg tcgattttgc
     1441 cttcgcttac gttctgcctg atttggttca tcaggtttgg atcaacttta gatgcttttc
     1501 ggatagccaa aaagcggtca acaaaaatga aaacagcgat taacgataaa gcaagaattg
     1561 gaatcatgat ccagcctcct ttgagtgcca attcgccata attcatggta actccagctt
     1621 ggtttacagc cagaactcct attgtctggt ttacttgtgg tagaacaaaa tctaacatat
     1681 ttttgtattt tactgtttct ttaaacgata atttggtgaa attattctct tttttcttta
     1741 ttgtcgcaaa gtaaataaag ttattgaatt tcgaaatggt taattttcgt ttttaggccc
     1801 tgtttttttc ttcttttttt tcagaattgc gttgcggtat tttctgaaca aatcttttaa
     1861 cgtattgaat tcttcgcgat acgataaacc gatgccttgt gtgtaaggtt cttcttcatt
     1921 caaaatgccg tcatttgaat gattataaac ttttaattgc agccggccgt cgtttgtaag
     1981 cttaacttta atatcgaatt cccctatgaa ttccccactg ttgttggccg atgggttact
     2041 gttgttgcct atatttccat cgatagttac cctatcgtta aatacctgag tactaagtgc
     2101 cagttcaatc tggtcgttcg atatttcttt aacaccgtcg gttacgcctg gccggtagtt
     2161 aaccccaata tctacctgat cgcttatttg agataaccag ttgctaagct ggttcgaaag
     2221 aagttcgctt gctgtggtgc ttgctgtatt tgttacaatt tcggagcctg caactgtagt
     2281 ggtttttcca gcatatatct caggagtata gaaacggcca agcattagta acgaaagcat
     2341 ttgtttgttt acatcttctt cggtaacaat gatttgtttt acctggtctt gaatactttg
     2401 ctcggctgtt ggcagttcga tcttaaagtt gattttaggt tgcatgaggc tttcactcaa
     2461 agatattaca cagtcgacgg gcatacggcg tacaagatcc atatcggtac tgctttcggg
     2521 aaacaaatcg tagagcgatg tttttacctt atatactgct gttaagttga tatctgcatc
     2581 gtagggctca cctgtccatt ttatagtgcc accttggtta atggtaaaac gcttattaat
     2641 aatgttttcg agagtgaaaa gataatctcc tttttcgatt acataatccc catagagttt
     2701 gatattgaaa tctttatcga ctttaa
//
LOCUS       DAOPUW010000235         2722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_38868_length_2722_cov_2.581552, whole genome shotgun sequence.
ACCESSION   DAOPUW010000235 DAOPUW010000000
VERSION     DAOPUW010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38868_length_2722_cov_2.581552"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PKJ43_04880"
     CDS             complement(<1..414)
                     /locus_tag="PKJ43_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71933.1"
                     /translation="MLELFNSNDFKLWSTGKLDREDLYYYKLKDEDPGWAKVDITRKA
                     NKIKIVLPLNLVPEQQMDFAFLQLMTSTTAACNSNFDNLMIPFRCVSTDIYNNKAIIH
                     RDGDIGEAIRASMTFPLVYKPIEKDGMLLFDGGIVN"
     gene            complement(439..636)
                     /locus_tag="PKJ43_04885"
     CDS             complement(439..636)
                     /locus_tag="PKJ43_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNZ71934.1"
                     /translation="MKKIYLTIFLFIIYTIANAQQVGLVLSGGGAKGLAQIGLIKVLE
                     DHNIPIDYVSGTSIGAIIGGL"
     gene            complement(652..1575)
                     /locus_tag="PKJ43_04890"
     CDS             complement(652..1575)
                     /locus_tag="PKJ43_04890"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNZ71935.1"
                     /translation="MKLIPKESIFSARNFRDYIFIVVGAFILAVGFVYFISPHRIVPG
                     GVYGIAIVVHYLTQGVFPFWPEGIPIGLFGLVLNIPLTYAGIKILGPKFGIKTIAGFV
                     LSSVFMDGITMLREVGDAPLVDDVLLSCVFGGVLIGFGLGLIFKSRATSGGSDIIAMI
                     IAKYTNRQLGQLMIYVDSAIVLIGLAAFQDWKIPLYSWLVIYITGKAIDATIEGSGYN
                     KALFIISKEHDKIKEKILFDLERGGTYFNGKGMFTNEEKQIIYTVVSRREVAILEQYI
                     HSIDPEAFITVMDTSEILGEGFQSLKHKIEP"
     gene            complement(1876..2235)
                     /gene="rplS"
                     /locus_tag="PKJ43_04895"
     CDS             complement(1876..2235)
                     /gene="rplS"
                     /locus_tag="PKJ43_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNZ71936.1"
                     /translation="MDAIKIVQNAFAQEEVKTHPEFGAGDTITVHYKIKEGNKERIQN
                     FRGVVLQVKGSGMTKTFTVRKMSGNVGVERIFPLNSPFIELIEINKKGKVRQARIFYF
                     RELNGKKARIKAKRIIQ"
     gene            complement(2377..>2722)
                     /locus_tag="PKJ43_04900"
     CDS             complement(2377..>2722)
                     /locus_tag="PKJ43_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNZ71937.1"
                     /translation="QAQLENSFVKTVKAIDQAALSLWNSNEKELALDVLTNYSVSTAN
                     KTVDRWRELGHFLLVKYKDGNIMKEKDGVFIRTKTGQAEYPDQPGYPDSWKRQVVKES
                     GEKLKMIGSAGH"
BASE COUNT          772 a          551 c          533 g          866 t
ORIGIN      
        1 atttacaatt cctccatcga atagcaacat tccatccttt tcaataggct tgtataccag
       61 ggggaaagtc attgatgcgc gaatggcctc accgatatct ccgtcgcggt ggatgatggc
      121 tttattgttg taaatgtcgg tcgaaacgca acggaaagga atcatcaggt tgtcgaaatt
      181 gctgttacaa gctgcagtag ttgaggtcat caactgaaga aaagcaaaat ccatctgttg
      241 ttcgggaaca aggttcagtg gcagaacaat ttttatttta tttgccttac gggtaatatc
      301 cactttagcc cagcctgggt cctcatcttt cagtttgtaa taatacaggt cttctctgtc
      361 gagttttccg gttgaccata acttaaaatc gtttgaattg aaaagctcaa gcatttcgtc
      421 gggtgaaaag ccagctgctt ataggcctcc gattatggca ccgatagatg tgcccgaaac
      481 ataatcgata gggatgttgt ggtcttcaag aactttaatc aagccaatct gtgccagtcc
      541 cttggcacca cctccactta acacgaggcc aacttgctgg gcgtttgcaa ttgtgtaaat
      601 aataaaaaga aaaatagtaa ggtagatttt tttcatggct aataaaatta cttagggttc
      661 aattttgtgt ttcagcgatt ggaagccttc gccaagaatc tcgctggtat ccatcacggt
      721 aatgaaagct tcgggatcaa ttgaatggat atattgttcg agaattgcca cttcgcgccg
      781 gctaactact gtgtatatta tttgcttttc ttcgtttgtg aacatccctt tcccattgaa
      841 ataggtgccc cctcgttcga gatcgaaaag tattttttct tttattttgt cgtgttcttt
      901 cgatatgatg aaaagtgctt tattatagcc acttccttca attgttgcgt cgatggcctt
      961 accagtaata taaattacca gccacgagta aaggggtatt ttccagtctt gaaaagctgc
     1021 aaggccaatc agcacaatgg cagaatcgac ataaatcatg agttgcccca actgtcggtt
     1081 ggtatatttt gcaataatca tggcaattat atcagacccc cctgacgttg ccctcgattt
     1141 aaaaataagc cctagcccga agccaataag cacgcctcca aaaacacacg aaagcagtac
     1201 atcgtcgacc agtggggcgt cgccaacttc gcgaagcatt gtaatgccgt ccataaaaac
     1261 cgaagaaagt acaaaacccg caattgtttt tatgccgaat tttggtccca gtattttaat
     1321 gcctgcatac gtgagcggaa tgtttaacac aagcccgaac aagccgattg gaatcccctc
     1381 gggccaaaac gggaaaacgc cctgggttag gtagtgaact acgatggcga tgccgtaaac
     1441 gcccccggga acaattctgt gtggtgagat gaaataaaca aagcctacag caagaatgaa
     1501 tgccccaaca acaataaaaa tataatcccg aaaatttcgg gctgagaaaa tcgattcttt
     1561 aggtattaat ttcatcgttg tttgtcttta attgttttag cctaaatata ataatgaaac
     1621 aattggcgtc gattgaactc caggggctaa tttttatctt tcagaatatc attttgtcgt
     1681 tttgaaaaag gttatttttt cctccactaa attaattata aagaaaatta cctaaaaatg
     1741 aatttcggca ttttgttgat ttcttttgaa ttgtataaga ttacggtgaa gtctgcctgt
     1801 gtacgtaact atgttgttat aaaaaaaaat ccccgcacca taaggtgaag ggattcagat
     1861 attgaaatat aacccttatt gtataattct ttttgctttg atacgtgctt tctttccgtt
     1921 caattcgcgg aaatagaaaa tacgagcctg acgaacttta cctttcttgt tgatttcaat
     1981 tagttcaata aaaggtgaat tcaaagggaa aatacgttcc acaccaacgt tacccgacat
     2041 tttgcgaacg gtaaaagttt tggtcattcc tgagccttta acctgaagca caacgccacg
     2101 gaagttctgg atacgttctt tatttccttc tttaattttg tagtgaacag tgatggtatc
     2161 tcctgctcca aactcagggt gggtcttcac ttcttcctga gcaaatgcat tttgtacaat
     2221 tttaatagcg tccattttta ttatttttaa tgggtttaaa cgcaatatta cctaatgtga
     2281 cctgaatggt aagagattac gtttgcctta aaggcggcgc aaaagtaaat attttttttt
     2341 agttgagaaa aggatatcat taattaattc agttttttaa tgtccagcac ttcctatcat
     2401 ttttaacttt tctccgcttt ctttcacaac ttgtcgtttc caactatcgg gataccctgg
     2461 ctgatcggga tattcggctt gccctgtttt ggtacgaata aataccccgt ctttttcttt
     2521 catgatattg ccatctttgt acttcactaa caggaaatga cctaattctc tccatcgatc
     2581 aacagttttg ttggctgtgc tgactgaata gttggtgaga acatccagag caagttcttt
     2641 ttcgttgcta ttccataatg aaagggctgc ctggtcaatg gccttcaccg ttttgacaaa
     2701 tgaattttcg agttgagcct gt
//
LOCUS       DAOPUW010000236         2720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_38898_length_2720_cov_3.171107, whole genome shotgun sequence.
ACCESSION   DAOPUW010000236 DAOPUW010000000
VERSION     DAOPUW010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38898_length_2720_cov_3.171107"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="PKJ43_04905"
     CDS             <1..1013
                     /locus_tag="PKJ43_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNZ71938.1"
                     /translation="YKRAELNYEQTQKRLKREELNLVYNVSNAFYSLLSLQKREDIAR
                     MNLERQKEAYTTAKNKYDAGLIREVDALQMEVDLAEAQNNYDVAIINRQASLNNFKEI
                     IGVSLQDSVILSSNLDYKIVTVDAGKAVELAMLNRSEIREQEIRLQLNKIDIKRQKSY
                     GMATGNITAHYERIGTNEGMMSNDISGAVNNTFNDFRNRPENFGIGFTVNIPIIDWGE
                     NRALVKAAEARLQQNMYQQEVVKRNIESEVMNLVADLNSSLKRLQLLEKNVSVAEKSF
                     EITRARFSDGDIDSQALALERDRLNNAYVTHLSAYINYQLKIADLMRKTFYDFQNDRP
                     VQ"
     gene            1250..1507
                     /locus_tag="PKJ43_04910"
     CDS             1250..1507
                     /locus_tag="PKJ43_04910"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNZ71939.1"
                     /translation="MKAVNYSELRQNLKQNLDAVSDNNDLLVVHRSKGKSIVMMSIDE
                     YNSMEETFHLLKSKANRDRLEAAIENINTKTNLVTYPLIEE"
     gene            1511..1765
                     /locus_tag="PKJ43_04915"
     CDS             1511..1765
                     /locus_tag="PKJ43_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002452576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HNZ71940.1"
                     /translation="MDLFWDKTAWDDYQYWINTDRKVLLKINSLIKECQRTPFIGTGK
                     PEPLKQNLSGFWSRRIAGEHRLVYKVENDVLFIAQCRYHY"
     gene            complement(2221..2700)
                     /locus_tag="PKJ43_04920"
     CDS             complement(2221..2700)
                     /locus_tag="PKJ43_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ71941.1"
                     /translation="MGQVYWLKKGELEFKEAISENFIIKKPPLCEDEAEGLISIQEEV
                     FSEISEQINILTKKGLICSGEHALNGLLYCLVKNDKDEYIGYGYGYFDFDNKYIFYID
                     TIGVKNESRRKDIGTKITVKLAQEAFLNPNIERVKVITQEDNKSAIAIIEKLGFSPY"
BASE COUNT          887 a          536 c          514 g          783 t
ORIGIN      
        1 gctacaaacg agctgagctg aactatgaac aaactcaaaa acggctaaag cgcgaggaac
       61 tgaaccttgt ctacaacgtg agcaacgcgt tttacagtct gctgtcgtta caaaagcgcg
      121 aggacattgc ccgcatgaat ctcgaacgcc agaaagaagc atacaccaca gcaaaaaaca
      181 aatacgacgc cggattgatt cgagaggtgg acgccctgca aatggaagtt gacttggccg
      241 aagcccaaaa caattacgac gtagccatca tcaaccgaca agcttcgcta aacaacttca
      301 aagaaatcat tggggtttca ttgcaggaca gtgttatttt gagcagcaac ctcgattata
      361 aaattgtgac cgtagatgcg ggcaaggccg ttgaactagc catgctaaac cgttccgaaa
      421 ttcgcgagca agaaatcaga ttgcaattga acaagatcga catcaaaagg cagaaatcgt
      481 atgggatggc aacaggcaat attacagcac attatgaacg cattggcacc aacgaaggta
      541 tgatgtcaaa cgatatatcg ggagcagtga ataatacctt caacgatttt cgcaatcgcc
      601 cggagaattt tggtattggc tttacagtga acatccccat tatcgactgg ggcgaaaacc
      661 gcgcattggt aaaggccgcc gaagcccgat tgcagcaaaa catgtatcag caggaagtgg
      721 tgaaacgcaa catcgaaagc gaagtgatga acctggtagc cgacctgaac agtagcttga
      781 aacgcttgca attgttggag aagaacgtat cggtggccga gaaaagtttc gagattaccc
      841 gcgcccgctt ctccgatggc gacatcgaca gccaggcatt ggccctggag cgcgaccgtt
      901 tgaacaacgc ctatgtgacg cacctctcgg cctacatcaa ctaccagttg aaaattgccg
      961 acctgatgcg caaaaccttt tacgatttcc aaaacgacag gccagtgcaa taaaagatgt
     1021 tcaaactttt taaaaagaaa gccctttgat tacagaaaat caaagggctt tttagtaccc
     1081 ggggcaggaa tttttaataa tttaatgtgt atttctttag tctgcttaaa tgttgattat
     1141 cagttaccaa ttgaatatga gtgagatggt aaaggcatgg aatttttgat ttgaaaatta
     1201 caaataaagt acttaatttt gtactcaata taaatataaa actgatattg tgaaggctgt
     1261 taattactct gaattgaggc aaaacctaaa acaaaatctc gatgctgttt ccgacaacaa
     1321 cgatttactg gtcgttcacc gatcgaaggg taaaagcatt gtaatgatgt cgattgatga
     1381 atacaactct atggaagaga catttcatct gctcaaaagc aaggccaacc gcgataggct
     1441 tgaggctgct attgaaaaca tcaacaccaa aacaaatctt gtaacgtacc cattaattga
     1501 agaataacca atggatttgt tttgggataa aacagcgtgg gacgattacc agtattggat
     1561 taacaccgac cggaaagtac ttctaaaaat taacagcctt attaaggaat gccaacgcac
     1621 accatttatt ggtacgggta agccggaacc gttgaaacag aatttatcgg ggttttggtc
     1681 acgccgtatt gcaggggaac accgactggt gtataaagtt gaaaacgatg ttttgtttat
     1741 tgctcagtgc agatatcatt attgaccatc acttctattg accgtgaaag ccagaatgaa
     1801 gcagttaaat tggctaaata aaaaacaaaa ccctctcaat tctagtgaat taagagggtt
     1861 tttagtaccc ttaggggaac ttttttccga accatttttg aatgatttag agttaatatg
     1921 gaatttgaaa aacactttaa aatcttcttt tttaattgaa taaatgacaa aacattttgt
     1981 agcattactt gggaataaca cataatattt caattttttc aggagcaagt atcatacaca
     2041 tattcgctcc tattatcttg ccatcttttg tttcgactaa aaaactgact ttattatcaa
     2101 gtccatttag gtactgactt ataaccaggc aatacttgtt tttgaaaagt cctaatttag
     2161 cgatcaatct accgttagaa tttgcgatag taaaattccc tataggctat aaaaccaatt
     2221 ttaatatggg ctgaagccta acttttcaat aattgcaatt gcacttttgt tatcttcttg
     2281 tgtaattact ttaacacgtt caatattcgg atttaaaaag gcctcttgag caagttttac
     2341 tgtgatttta gttcctatat cttttctcct tgattcattt ttaacaccga tagtatcaat
     2401 gtaaaaaatg tatttattat cgaaatcaaa gtaaccatat ccatatccga tgtattcatc
     2461 cttatcgttt ttgaccaaac agtataataa accattcaat gcatgttcac ctgaacatat
     2521 taatcctttc tttgtcaaaa tgtttatttg ttcacttatt tctgaaaaaa cttcttcttg
     2581 aatcgagatc aaaccctcag cttcatcttc acaaagtggc ggctttttta tgataaaatt
     2641 ttccgaaatt gcttctttga attctaactc cccttttttc aaccaataga cttgtcccat
     2701 agcaaattgt atttttcagt
//
LOCUS       DAOPUW010000237         2715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_39007_length_2715_cov_2.737218, whole genome shotgun sequence.
ACCESSION   DAOPUW010000237 DAOPUW010000000
VERSION     DAOPUW010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39007_length_2715_cov_2.737218"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PKJ43_04925"
     CDS             complement(<1..259)
                     /locus_tag="PKJ43_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71942.1"
                     /translation="MKPNVIIVILIALIATACGTSNQMTGRYVDDLYYWPGDTPPVAV
                     TQEVPATKSADSITQRDMLIITEVGEDEKGNKTLDNYIYADD"
     gene            365..1840
                     /gene="proS"
                     /locus_tag="PKJ43_04930"
     CDS             365..1840
                     /gene="proS"
                     /locus_tag="PKJ43_04930"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNZ71943.1"
                     /translation="MAKELTSRKENYSQWYNDLVIKADLAENSAVRGCMVIKPYGYAI
                     WEKMQHELDRMFKETGHVNAYFPLFIPKSFFSKEASHVEGFAKECAVVTHYRLKNDPN
                     GKGVVVDPDAKLEEELIVRPTSETIIWNTYKNWIHSWRDLPILCNQWANVVRWEMRTR
                     LFLRTAEFLWQEGHTAHATKEEAMEETMKMVNVYADFAENFLALPVIKGHKSENERFA
                     GALDTLCIEALMQDGKALQAGTSHFLGQNFAKAFDVKFADKEGKLDYVWATSWGVSTR
                     LMGALIMAHSDDNGLVLPPKLAPYQVVIVPIYKEMDQLASISSKVEGIIKALRAKGIS
                     VKYDDNDAKKPGWKFAEYELKGVPVRLALGPRDLENGTIEVARRDTLTKEVVPIENVD
                     VYVADLLEEIQKTIYNKALEFRAASTTSVNTWEEFKEVLVNKGGFISAHWDGTPETEQ
                     KIKEETKATIRCIPLDAVEEEGKCIYSGKPSKCRVLFAIAY"
     gene            complement(1900..>2715)
                     /locus_tag="PKJ43_04935"
     CDS             complement(1900..>2715)
                     /locus_tag="PKJ43_04935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ71944.1"
                     /translation="NPIVRDIEKDDKGNIWFGTENGICVSTQASNINIVPKSSNRASI
                     SWTSGKGFVTALPNEYINDLQKDEEGKMWIATNNGVRILNAVPESSNTSTIKRVVFIS
                     ESIVQLLTPFNWTTYTANAEYKKGSAIGTWYCIYNGTGTSVEVTGLSPNTTYWVAVFD
                     YQGEPGFETYSIAGGEGNPESFLSLPVSADEFTRGEIRAYPVPFNEYLNISFEGEKGK
                     TYEITISDLEGKVFKKVKANGITDKINTSDLSRGAYMLKVSDGKQERTIKVIK"
BASE COUNT          736 a          511 c          595 g          873 t
ORIGIN      
        1 gatcatcggc atagatgtag ttgtcgaggg ttttgtttcc cttttcatct tcacctactt
       61 cggtgattat gagcatatca cgttgggtga tgctgtcggc acttttggta gccggcactt
      121 cttgtgttac agctacggga ggggtatctc cgggccagta atataggtcg tcgacatacc
      181 gccccgtcat ttgattgctt gttccacagg ctgtggcgat caaagcgatc aggattacta
      241 tgattacatt aggtttcata aacttgtcct cctttgactt tatttatttt attttttttg
      301 tgtttctttg tacgtgcttt tctgcacaaa aaatgaaaca aatattatac caaagaagct
      361 tataatggcg aaggaattaa cttcaaggaa agaaaattat tctcagtggt acaacgatct
      421 ggttataaag gccgatttag ctgagaattc tgctgtgaga ggttgtatgg taatcaaacc
      481 ttatggttat gcaatctggg aaaaaatgca gcacgaactc gatcgtatgt ttaaagaaac
      541 aggccacgta aatgcctatt tcccattatt tatcccaaaa tcgtttttta gcaaagaggc
      601 cagccatgta gaaggttttg ctaaagaatg cgcagtggtt actcactacc gtttgaaaaa
      661 cgatcccaat gggaagggtg ttgtggtcga ccctgatgca aagcttgagg aagagcttat
      721 tgtcaggcct acatctgaaa cgattatttg gaatacctat aaaaattgga ttcattcgtg
      781 gcgtgatctt ccaattttgt gcaaccaatg ggcaaacgtt gttcgctggg aaatgcgtac
      841 ccgtttgttt ttgcggactg ccgagttttt atggcaggaa gggcatactg cccacgcaac
      901 gaaggaagag gcaatggaag aaactatgaa aatggttaat gtatatgccg actttgctga
      961 aaattttctt gctcttccgg ttattaaagg tcacaaatcg gaaaatgaac gttttgccgg
     1021 agctctcgac actctttgta tcgaagctct gatgcaggat ggaaaagccc tgcaggctgg
     1081 aacttctcat tttcttggtc aaaatttcgc caaagcattc gatgtgaagt ttgctgataa
     1141 agaaggaaaa cttgattatg tttgggctac atcgtggggc gtttctaccc gattgatggg
     1201 tgcgcttata atggctcatt ccgatgataa cgggttggtt cttcctccaa aacttgctcc
     1261 ttatcaagtg gttattgttc ctatatataa agaaatggat caacttgcat caattagctc
     1321 aaaagttgaa ggaattatta aagcattgcg cgctaaaggc atatcggtga aatacgatga
     1381 taatgatgct aaaaaaccag gatggaagtt tgctgaatat gagttgaaag gtgttcctgt
     1441 tcgcctggca ttgggccctc gcgatctcga aaatggcaca attgaagttg cccgtcgtga
     1501 tacacttacc aaagaggtgg tgcccatcga aaatgtagat gtatatgtcg ctgatttgct
     1561 tgaagaaatt caaaaaacta tttacaataa ggccttggaa tttagggctg cttcaacaac
     1621 gtcagttaat acatgggaag aatttaaaga agtattggtg aataagggcg gatttatctc
     1681 ggctcattgg gatggcaccc ccgaaaccga acagaaaatt aaagaagaaa caaaagctac
     1741 tattcgttgt attccactcg atgctgttga ggaggaagga aaatgtattt attccggaaa
     1801 accttcgaag tgcagggtgt tgtttgctat tgcttattag ttttttaggt ttcgatatat
     1861 aaaaaagtaa gtcccggaaa atttccggga cttacttttt tatttgatta ccttaatggt
     1921 tctttcctgt tttccgtcgc ttacttttaa catgtatgct cctctgctca agtctgatgt
     1981 atttatttta tcagttattc cattagcctt cacttttttg aacacttttc cttcgaggtc
     2041 tgagatggta atctcatacg ttttgccctt ctctccctca aaagaaatat ttagatattc
     2101 gttgaatgga acagggtaag ctctaatttc ccctcgtgtg aattcatcag cacttactgg
     2161 gagtgataaa aaactttcgg ggtttccctc gcctccggca atagagtatg tttcgaaccc
     2221 tggttctccc tgataatcga agactgcaac ccaataggtt gtgtttggtg agaggcccgt
     2281 aacttcaaca ctagttccag ttccattgta gatacaatac caggtgccaa ttgctgaacc
     2341 ttttttgtat tcagcatttg ctgtgtatgt tgtccagttg aatggagtca atagttgtac
     2401 aatcgattcg gagatgaaaa caaccctttt tattgttgac gtattagaag attctggaac
     2461 ggcatttagt attctaacgc cgttgtttgt agctatccac atttttcctt cttcgtcttt
     2521 ctgaaggtcg ttaatatatt cattcgggag tgccgtgaca aaacctttcc ctgaggtcca
     2581 ggagattgaa gctcgattcg atgattttgg cacgatgttg atgttcgatg cttgtgttga
     2641 cacgcaaatt ccattttcag ttccaaacca gatattccct ttgtcatctt tttcaatatc
     2701 acgaacaatt ggatt
//
LOCUS       DAOPUW010000238         2708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_39163_length_2708_cov_2.941199, whole genome shotgun sequence.
ACCESSION   DAOPUW010000238 DAOPUW010000000
VERSION     DAOPUW010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39163_length_2708_cov_2.941199"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /locus_tag="PKJ43_04940"
     CDS             <1..1483
                     /locus_tag="PKJ43_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNZ71945.1"
                     /translation="PNRILLISGPNAGGKSVCLKSVGIIQYMLQCGLLVPMADGSKMG
                     IFEAIFIDIGDEQSIENDLSTYSSHLMNMKFFTRNCNKRTLVLIDEFGTGTEPMLGGA
                     IAEAVLNKLNKLGTYGVITTHYTNLKHFGAAEKGIVNGAMLYDSGQMRPLFKLQIGNP
                     GSSFAFEIARKIGLSEEILNEATEKLGKEHIDYDKHLREIVRDKQYWESKRQKIKETE
                     RKLDNLSEEYSSELQQVQKQRKQIIAEAKQEAQKILDDANKMIENTIREIRESQAEKE
                     KTREIRKSFTEHREKIDQIETETSAKIAQQMERIKQKQNREKKPKEVQKTEPKPVQKP
                     KFNRGDKVKLKENGNIGEIIEIDKNKVVLALGNLMTTMGIDKIEPISNNEAKKIEKET
                     RMVPKADTLKRTNEMMAKRLTFKPDIDVRGQRGEEALANIQSFIDESIMLRQKTLRIL
                     HGKGYGILKEIIRNYLRTEPAVHSFRDEDVQLGGAGITVVELE"
     gene            1515..>2708
                     /locus_tag="PKJ43_04945"
     CDS             1515..>2708
                     /locus_tag="PKJ43_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNZ71946.1"
                     /translation="MSNSQYEKKLKALAAQLEGELRFDSISKILYATDASAYREMPLA
                     VAIPASKNDIAKLISFARENNTSLIPRGAGTSLAGQVVGHGIVVDVSRNIDKIIEINR
                     EEHWARVEPGVVLAELNSKAKAMGLQFGPETSTATRCTMGGMLGNNSCGLHSLVYGSV
                     REHIIETTAFLSDGSEVVFKELTQTEFEDKCKEKTLEGEIYRQIKNILSDKKNLEEIE
                     NQFPDKTVPRRNNGYALDQLSQMQPFNPDGKKINLAKFIAGSEGTLAFVTEMKLNLVP
                     LPPKEKALLCVHFEQLTDAFQANLIGLKYKPWAIELMDKTISNLTKENIGLKRNRFFI
                     EGEPAAILIIELCGDTKEELISTAKEIEKEMLANKLGYHFPMIWGNDIPKVWGLRTAG
                     LGVLSN"
BASE COUNT          980 a          561 c          571 g          596 t
ORIGIN      
        1 acccaaccgc atcttgctga tttcggggcc caacgctgga ggcaaatcag tatgtctcaa
       61 gtcggtagga ataatacagt atatgttgca atgcgggttg cttgttccaa tggccgacgg
      121 ttcaaaaatg ggcatcttcg aagcaatttt tatcgatatt ggcgatgaac aatccatcga
      181 aaacgacctc agcacctact catcgcacct aatgaacatg aaattcttta cccgtaactg
      241 caacaaacga acgctagtac taatcgatga gtttggaaca ggaaccgaac caatgcttgg
      301 aggcgcaatt gcagaagcag tgcttaataa acttaacaaa ttgggtacgt acggggttat
      361 caccacccac tacaccaatc tgaaacactt tggtgcagct gagaaaggaa ttgttaacgg
      421 ggcaatgcta tatgattcgg gacaaatgag accccttttc aaattacaaa ttggtaaccc
      481 gggaagttct tttgcttttg aaattgctcg aaaaatcggg ctttcggaag aaatcctgaa
      541 tgaagccacc gaaaaacttg gaaaagaaca catcgattac gacaaacatt tgcgggaaat
      601 cgttcgtgac aaacaatact gggaatcgaa aagacaaaaa ataaaagaga ccgaaagaaa
      661 actcgacaat cttagtgaag aatactcaag tgagctgcaa caagttcaaa aacagcgaaa
      721 acagattatt gccgaggcca aacaggaagc tcagaaaata ttggacgatg ccaataaaat
      781 gattgaaaac accattcgtg aaattcgcga atcacaagcc gagaaagaaa aaacccgcga
      841 aatcagaaaa agcttcactg agcatcgtga aaagatagac caaatagaaa cagaaacttc
      901 ggcaaaaatt gcacaacaga tggagcggat aaagcaaaaa caaaaccgcg aaaaaaagcc
      961 taaagaagtg caaaaaactg agccaaaacc agttcaaaaa ccgaaattca accgtggcga
     1021 caaagttaaa ctgaaagaaa acggcaacat tggcgagatt atcgaaatag acaaaaacaa
     1081 agtagtattg gcattaggta atttaatgac tacaatgggg atcgataaaa ttgagcccat
     1141 aagcaacaat gaagcaaaaa agattgaaaa agaaacgcgc atggtcccca aagccgacac
     1201 attaaaacga acgaatgaaa tgatggccaa acgcctcacc ttcaagcccg atattgatgt
     1261 tcggggtcag cgtggagaag aagcccttgc caacattcag tcatttatcg acgagtcgat
     1321 aatgttgcgt caaaaaaccc tcagaattct gcacggcaaa ggatacggga tccttaagga
     1381 aattatccgt aactatctgc gcaccgaacc agcagtgcat tctttccgcg atgaagatgt
     1441 acaattgggc ggagcaggaa ttaccgtggt cgaactggaa taaacacaat ctttctacat
     1501 tgattaacaa caccatgagc aactcacaat acgaaaaaaa gcttaaagca cttgctgctc
     1561 agctcgaggg agaacttcgc tttgattcta tttctaaaat attatatgca accgacgcat
     1621 cagcctatcg tgaaatgcca ttggcggttg caatcccagc atcgaaaaac gacattgcaa
     1681 aactcatatc atttgcccgc gaaaacaata catcgcttat cccgcgcgga gcaggcactt
     1741 cacttgccgg acaagtagtt ggccatggta tcgtagttga tgtatcaagg aacattgaca
     1801 aaataatcga gataaaccgt gaagaacatt gggccagagt tgagccggga gttgttttag
     1861 ctgaactcaa ttcaaaagcc aaagcaatgg ggctgcagtt tggtcctgaa acatcaacag
     1921 ctacccgatg taccatgggc ggaatgttgg gaaacaacag ctgtggactg cactcgcttg
     1981 tctatggcag tgtgcgtgaa cacattattg aaacaactgc atttttaagc gatggttcag
     2041 aggtcgtttt taaggagctg acacaaaccg aatttgaaga taaatgcaaa gaaaaaaccc
     2101 ttgaaggaga aatttacagg caaataaaaa acattttatc cgacaaaaaa aatctggaag
     2161 aaattgaaaa tcaatttcca gacaaaactg ttccaagaag gaataacggg tacgcactcg
     2221 accagctatc acaaatgcag ccttttaatc ccgatggaaa aaaaataaat ctggccaaat
     2281 tcattgcagg ttccgaaggt acattagctt ttgttaccga aatgaaacta aacctggttc
     2341 cgctgcctcc aaaagaaaaa gcgctgctct gcgttcattt tgaacagctc actgatgctt
     2401 ttcaagccaa tctcatcggc ttaaaatata aaccatgggc aattgagctg atggataaaa
     2461 ccatcagcaa cctgacaaaa gaaaatattg ggctgaaacg taaccgcttt tttatagaag
     2521 gcgaaccggc tgcaattctg atcatcgaac tttgtggcga cacgaaagaa gaactcattt
     2581 caactgcaaa agaaattgaa aaagaaatgc ttgccaataa actagggtat catttcccaa
     2641 tgatttgggg aaacgatatt ccaaaagttt ggggtctgcg tacagcaggg ttaggggtcc
     2701 tttcaaat
//
LOCUS       DAOPUW010000239         2698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_39370_length_2698_cov_2.522134, whole genome shotgun sequence.
ACCESSION   DAOPUW010000239 DAOPUW010000000
VERSION     DAOPUW010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39370_length_2698_cov_2.522134"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..450
                     /locus_tag="PKJ43_04950"
     CDS             184..450
                     /locus_tag="PKJ43_04950"
                     /inference="COORDINATES: protein motif:HMM:NF016855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNZ71947.1"
                     /translation="MKSDLSKHTNIGKDTEAKLIQVGIDSFEKLKSVGTEQAFIRLQT
                     IDPGACISLLYGLDGSLSDTKWNKLSQERKAELLQFYNMVKKDM"
     gene            450..1076
                     /locus_tag="PKJ43_04955"
     CDS             450..1076
                     /locus_tag="PKJ43_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71948.1"
                     /translation="MNVIDLLATSLNRSDDKPNQELADEIIKSKRTDWVKELIENLNS
                     KDKNIQSDCIKVLYEIGERGAASMIAPYFETFGNLLESKNNRLIWGAMYALDSIASVN
                     PAEIFSILPAICKAIDKGSVITIDGGVSILAKLAAVNDYTETTFPLLLEQLKRCPAKQ
                     LPMYAEKSLKAINASNKSLFIDLLRERYSEMEKDTQKKRLEKVLRSLK"
     gene            complement(1068..1415)
                     /locus_tag="PKJ43_04960"
     CDS             complement(1068..1415)
                     /locus_tag="PKJ43_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71949.1"
                     /translation="MSVREIGCCGAYCKTCMVFLTKGQCKGCKLGYEDGSRDLSKSKC
                     AYKNCCYRDHNLETCADCPDYSSCELIHGFYAKNGYKYKKYQQAVEFIRANGYETFLK
                     LADEWKGPYGKLF"
     gene            1521..1859
                     /locus_tag="PKJ43_04965"
     CDS             1521..1859
                     /locus_tag="PKJ43_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71950.1"
                     /translation="MLRLFRNSISILMIGVLFLPSIVKIEHHHDHFVCKAKTEKHIHD
                     HHEKCQVCSFEFSVFMSEEFFSLPENPISFGGFINDYKVGYFSTAPDYTFLLRGPPSL
                     FAQLTTTDTK"
     gene            1972..2238
                     /locus_tag="PKJ43_04970"
     CDS             1972..2238
                     /locus_tag="PKJ43_04970"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNZ71951.1"
                     /translation="MKKIIILCAMVLFVCTVVAQNKISGKVTNQHHAPLPGATVSLPE
                     LNKGTMTNEKGEYELTNLPNGKNKVQFSFVGYASDIKTVLLNNA"
     gene            2290..>2698
                     /locus_tag="PKJ43_04975"
     CDS             2290..>2698
                     /locus_tag="PKJ43_04975"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNZ71952.1"
                     /translation="MVSGGYHSTQHENAVKIDVLKLNPKEITATPNFTEVLTRVPGVD
                     MISKGSGISKPVIRGLSMNDILVLNNSVRYENYQYSSHHPLGIDEFGIEDVEVIKGPA
                     SLLYGSDAIGGVINFIKEKPAPQHTITGDYNLQL"
BASE COUNT          826 a          590 c          576 g          706 t
ORIGIN      
        1 attaaagctg aaacagaaag taacaacatt gcatcaataa gggtgcttga aaagaatggt
       61 ttcaaaatca atcaacagac ggagggtggt ttaatcttta aattagaatt gaaatagaaa
      121 ccctgttgac atacagttgt caaaccagtg ttataaattt gcactaacgg taaataattg
      181 aaaatgaaaa gcgatttgtc gaaacatacg aacattggga aagataccga ggccaaacta
      241 attcaggtag gtatcgattc ttttgagaaa ttgaaatcgg taggaacaga acaggctttt
      301 atccggcttc agaccatcga ccccggggca tgtatatcct tactttatgg gttggacggt
      361 tcgttgagcg acaccaaatg gaataagctt tcgcaggaac gtaaagccga attgctacaa
      421 ttttataata tggtaaaaaa agatatgtga tgaatgtaat tgacttactg gccacatcgc
      481 ttaaccgtag cgatgacaag cccaatcagg aattggccga tgaaataatc aaatcaaaac
      541 gtacagattg ggtaaaggag ctaattgaaa acctgaacag caaggataaa aacatccaaa
      601 gcgattgtat caaggtgctt tacgaaatag gggagcgggg tgcggcttcc atgattgctc
      661 cgtacttcga aacatttggc aacctgctcg aaagtaaaaa taaccggctg atttggggcg
      721 ccatgtatgc gctggattcc attgcctcgg ttaacccagc tgagatattc tccattcttc
      781 cagccatctg caaagccatc gacaaaggtt cggtcattac catcgacggc ggcgtttcga
      841 ttctggcaaa gcttgcggca gtaaatgatt ataccgaaac cacatttcct ttgctacttg
      901 aacagcttaa gcgttgtcct gccaagcaac tgcccatgta tgccgaaaaa tcgttgaaag
      961 ccattaatgc ttccaacaaa agtctgttta ttgacttgct cagggaacgt tattccgaaa
     1021 tggaaaagga tacgcagaaa aagaggctcg aaaaagtatt gaggagttta aaatagtttc
     1081 ccatacggcc ctttccattc atcggcaagt tttagaaaag tttcgtagcc atttgccctg
     1141 atgaattcaa cagcctgctg gtattttttg tacttgtaac cgttttttgc atagaagcca
     1201 tggatcaatt cgcacgaact gtagtcgggg cagtcggcac aggtttcgag gttatggtcg
     1261 cggtagcagc agtttttgta ggcacatttc gatttcgaaa gatcgcgcga gccatcttca
     1321 taacccagtt tacagccttt gcactgccct ttggtcagga acaccataca cgttttgcaa
     1381 taggcaccac agcaaccaat ttcacgaacc gacatgactt tcagattatt aaaagtgcaa
     1441 tttagaaatt tattgtcaaa tcgatagaca ccgtgaacta aataaggctt tctttgtctt
     1501 ctgtaaacca actctttttt atgctcagac ttttcagaaa tagcatttca attttgatga
     1561 taggggtttt gttcctgcca tccattgtga aaattgagca ccatcacgac cattttgttt
     1621 gcaaagctaa aaccgaaaag catatacacg atcaccacga aaaatgccag gtttgcagct
     1681 tcgagttctc ggtttttatg tcggaagagt tcttttcact tcctgaaaac ccgatctcat
     1741 tcggaggttt tatcaacgac tacaaagttg gctatttctc aaccgctccc gattatactt
     1801 ttctgttacg gggccctcca tctttatttg cgcaattaac cacaacggac acgaagtaaa
     1861 cgcaaaggtt accaaatcag gaatcttgat acttgtgtcc tgatacttga tactttttca
     1921 gggcctcaac atcaattaag gctcattatt tatgtttaat ccgtaacaga aatgaaaaaa
     1981 ataatcatac tgtgcgcgat ggtgcttttt gtatgcaccg ttgtggcaca aaacaaaata
     2041 tccggtaagg ttaccaatca acaccatgcg cccctgcccg gggcaaccgt ctcgctgcct
     2101 gagttgaaca aaggcaccat gaccaacgaa aaaggcgaat acgaacttac caaccttcca
     2161 aacggcaaaa acaaagtgca gttttcgttc gtggggtatg cctccgacat taaaaccgtg
     2221 ctgctgaaca atgcctaggt cgaacttaat gccacactga aagaggcggt cattgaagcc
     2281 gaagaaattg tggtttcggg cggataccat tcgacacagc acgaaaatgc ggtaaaaatc
     2341 gacgtgttga aactcaatcc aaaagagatt actgcaacac ccaacttcac cgaagtgctt
     2401 acccgtgtgc cgggtgtcga tatgatttcg aaagggagcg gcatatcgaa acctgtgatc
     2461 cgcggccttt cgatgaacga cattctggta ctcaacaaca gcgttcgata cgaaaactac
     2521 cagtattcga gccaccaccc gctgggaatc gacgaatttg gcatcgaaga tgtagaagtg
     2581 atcaaaggcc cggcatcgtt gctttacgga tcggatgcca ttggcggggt aatcaatttc
     2641 ataaaggaaa aacctgcgcc ccagcacacc attacgggcg actacaactt gcagcttt
//
LOCUS       DAOPUW010000240         2693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_39459_length_2693_cov_3.619788, whole genome shotgun sequence.
ACCESSION   DAOPUW010000240 DAOPUW010000000
VERSION     DAOPUW010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39459_length_2693_cov_3.619788"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..238)
                     /locus_tag="PKJ43_04980"
     CDS             complement(29..238)
                     /locus_tag="PKJ43_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71953.1"
                     /translation="MMKDIIYKTENYKIIGTCMEVHNNLGPGFLEICDKSIAQALNYL
                     SVSGCKVSLIVNFGELSLNYKRIIK"
     gene            complement(376..720)
                     /gene="rplT"
                     /locus_tag="PKJ43_04985"
     CDS             complement(376..720)
                     /gene="rplT"
                     /locus_tag="PKJ43_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNZ71954.1"
                     /translation="MPRSVNNVASRARRKKLLKATRGNFGSRANVWTVAKNTWEKGMQ
                     YAYRDRKRKKGLYRALWIQRINAAVRPEGLNYSTFINLLKVKNIDINRKVLADLAMNH
                     PEAFKAIVDKVK"
     gene            complement(817..1014)
                     /gene="rpmI"
                     /locus_tag="PKJ43_04990"
     CDS             complement(817..1014)
                     /gene="rpmI"
                     /locus_tag="PKJ43_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012095718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNZ71955.1"
                     /translation="MPKMKTNAGAKKRFTLTGTGKIKRKHAFKSHILTKKSTKRKRML
                     THVGLVSADDLTNVKKMLAMK"
     gene            complement(1046..1582)
                     /gene="infC"
                     /locus_tag="PKJ43_04995"
     CDS             complement(1046..1582)
                     /gene="infC"
                     /locus_tag="PKJ43_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNZ71956.1"
                     /translation="MENNKDDDKLFFRINHQIRVREVRLVGDNIETPGVYPIQQALKM
                     ADELELDLVEISPKAEPPVCRIIDYQKFLYQQKKKQKEIKAKSVKVVVKEIRFGPQTD
                     EHDFQFKLRHAEKFLEEGAKVKAYVFFKGRSILFKEQGEILLLKLANELEEFGKLDQM
                     PKLEGKRMTIFISPKKKK"
     gene            complement(1632..>2693)
                     /gene="thrS"
                     /locus_tag="PKJ43_05000"
     CDS             complement(1632..>2693)
                     /gene="thrS"
                     /locus_tag="PKJ43_05000"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNZ71957.1"
                     /translation="PHIGDVNLYKTSGHYEKYGKDSFQPIHTPQEGEEYLLKPMNCPH
                     HCEIYKSKPRSYKDLPVRYAEFGTVYRYEMSGELHGLTRVRGFTQDDAHIFCTPDQLK
                     DEFKKVIDIIFIIFKALDFKEYTAQISLRDPNNNSKYIGSDENWEKAERAIIEAAAEK
                     GLKTVTETGEAAFYGPKLDFMIKDAVGRSWQLGTIQVDYNLPERFQLEYIGADDQRHR
                     PVMIHRAPFGSMERFVAVLIEHTMGKFPLWLTPEQVVVLPISEKFNDYARNVSNILNN
                     YDIRTVVDERNEKIGRKIRDNELKRTPYLLIVGEKEAETESVAVRRQGEGDKGTMTIE
                     EFARFIKEEIKEQLNAINI"
BASE COUNT          674 a          555 c          504 g          960 t
ORIGIN      
        1 ccgattgtgt tatcggaaag tttcgtgttc attttataat tcgtttgtaa ttaaggctta
       61 gctctccaaa attgacaatc aacgaaacct tacatcctga aacgcttaaa taattaaggg
      121 cttgtgcaat tgatttatca catatttcca gaaagcccgg accaaggttg ttgtggactt
      181 ccatgcatgt cccaataatc ttgtagtttt cagtcttgta aataatatcc ttcatcatta
      241 ttcgtgaatt tgtggccttt tagttagtta tgttacaaaa attaatttat ccactcgaat
      301 attagtaatc caaaaaaacc cgctgacttt gcaggcagcg ggctatatat atcacctaag
      361 attattttct gattgttatt ttactttatc aacaatcgct ttaaaagctt caggatgatt
      421 cattgctaaa tcagctaaaa ctttacggtt aatatcaata ttcttaacct tcagcaggtt
      481 gatgaatgtt gagtaattga gcccttcagg acgaacagca gcattgatac gctgaatcca
      541 taaagccctg taaagccctt tctttctttt gcggtcgcgg taagcatatt gcatcccttt
      601 ttcccaggtg ttttttgcta ccgtccacac atttgctctc gaaccaaagt taccacgcgt
      661 ggcttttaat aatttttttc ttctcgcgcg agaggctaca ttgttaacac ttcttggcat
      721 aatcttacat tttcgagtgg ttagcgtcat gcttcagcat gaactttggg ctaatacact
      781 ccggttaatt actaatttta ctttaaatca aaaaaactat ttcatagcga gcattttctt
      841 cacattggta aggtcgtcgg ctgacaccaa acccacatga gtaagcatgc gtttacgttt
      901 tgtgcttttt ttagtcaaaa tgtgactttt gaaagcatgt tttcttttga tttttcctgt
      961 accggtgagc gtaaaacgct tcttcgcacc ggcattggtt ttcattttcg gcattatcct
     1021 acttttttaa ttaatgtaca acctattatt ttttcttctt aggtgaaata aatatcgtca
     1081 ttcgtttgcc ttctaacttc ggcatttgat ctaattttcc aaattcttca agttcattgg
     1141 caagcttaag cagaaggatt tctccttgct ctttaaataa aattgaacgc cctttaaaga
     1201 atacgtaagc tttaactttt gccccttctt cgaggaattt ttcagcatgc ctgagcttga
     1261 actgaaaatc gtgttcgtcg gtttgtgggc caaaacggat ttcttttaca acaactttaa
     1321 ccgattttgc tttaatttcc ttttgtttct ttttttgctg ataaagaaac ttctggtaat
     1381 caataatgcg gcaaacaggt ggttctgctt tgggtgagat ctcaactaag tcgagttcta
     1441 attcatcagc catttttagt gcttgctgta ttgggtatac ccccggcgtt tcaatgttgt
     1501 cgcctaccag cctgacctcg cgcactctaa tttgatggtt gattctgaag aataatttat
     1561 cgtcatcttt attattctcc attcttttgt tttgtggtac tattgttgtg tcctcctaat
     1621 taagttagta atcaaatatt tatagcattc aattgttctt ttatttcttc ttttatgaag
     1681 cgggcaaatt cttcaatggt cattgttcct ttgtcgcctt ctccttgccg gcgaacggct
     1741 accgattcgg tttcggcttc tttttcacca acgataagaa gataaggtgt ccgtttaagt
     1801 tcgttatcac gaatctttct gcctatcttc tcgttacgtt cgtcaacgac ggtgcgaata
     1861 tcgtaattat ttaaaatatt tgaaacattt cgtgcataat cgttaaattt ttcactgata
     1921 ggcagtacca ccacttgttc aggggttaac cataacggga atttacccat ggtgtgctca
     1981 atcagaacgg ctacaaaccg ctccattgaa ccaaaaggag cacggtgaat cattaccggg
     2041 cggtggcgct ggtcatcagc tccaatgtat tccagttgga accgttcagg aaggttatag
     2101 tcaacctgaa ttgtgcctaa ctgccagctt cggccaactg catctttgat catgaagtcg
     2161 agttttgggc catagaaagc agcttcgcca gtttcggtta cggttttcaa ccctttttca
     2221 gctgccgctt cgataatggc tcgttcggct ttttcccagt tttcatccga cccgatgtat
     2281 ttgctgttgt tattagggtc gcgcaacgat atttgtgcag tatattcttt gaaatctaaa
     2341 gctttgaata ttatgaagat aatatcaatt acttttttga attcatcttt aagctgatcg
     2401 ggcgtacaga aaatatgcgc gtcgtcttgg gtaaagccac gaaccctggt tagcccgtgc
     2461 aattcaccac tcatttcgta acgataaact gttccaaatt cggcataccg aacaggcagg
     2521 tctttgtacg accgcggttt cgatttatat atttcgcagt ggtgagggca attcatgggt
     2581 ttcagaaggt attcttcgcc ttcttgtggg gtatgtatcg gttgaaatga atctttcccg
     2641 tatttttcat agtgcccaga ggttttgtac aggtttacat cgccaatgtg cgg
//
LOCUS       DAOPUW010000241         2692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_39488_length_2692_cov_2.991657, whole genome shotgun sequence.
ACCESSION   DAOPUW010000241 DAOPUW010000000
VERSION     DAOPUW010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39488_length_2692_cov_2.991657"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PKJ43_05005"
     CDS             complement(<1..361)
                     /locus_tag="PKJ43_05005"
                     /inference="COORDINATES: protein motif:HMM:NF027659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4968 domain-containing protein"
                     /protein_id="HNZ71958.1"
                     /translation="MKILKMAIIALLLSLQLQAADNFVKESNRVLLKTEKGTLAIYPL
                     TDNTVRVQFFTAENQTLPELVFESKPSVPEFNVIESKKSINTTLKNIVVELDKKTDKL
                     SFYDANGKLLLSEKANSP"
     gene            complement(419..658)
                     /locus_tag="PKJ43_05010"
     CDS             complement(419..658)
                     /locus_tag="PKJ43_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71959.1"
                     /translation="MGVFLGLTIQAQDNTPHPGLENSSIQGINKEWPRALFVSYESKE
                     LAMKGCPEASCMFASLNGTKAKCRRIELQLHHCSF"
     gene            complement(749..1114)
                     /locus_tag="PKJ43_05015"
     CDS             complement(749..1114)
                     /locus_tag="PKJ43_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71960.1"
                     /translation="MRQRGVQPGTVDLLLEENGIAKSIASVNLKEEVIGDKNLLLRLV
                     KAGSTYTAFYSTDGKTFEKLGASDIALKDIKAGLIACDGIITQSMTSTYWFNSDTTKP
                     ATPFDVSFDYFRIQNSGLK"
     gene            complement(1111..>2692)
                     /locus_tag="PKJ43_05020"
     CDS             complement(1111..>2692)
                     /locus_tag="PKJ43_05020"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HNZ71961.1"
                     /translation="DCRDIRPRLRLVAGVPAEGAEIIRRVPAAERKRMGMVEVEDIKN
                     NVNIPGIIYTGYNGQAQGAAMAKVLFGEVNPGGKTSVTWYKSVKDLPDFNDYNLRAAD
                     GKNGRTYWYFNKDVSYEFGFGLSYTTFEFSNFSISKSKISPNDKVTVSVDVKNTGDVD
                     GDEVVQIYVKTPESAASLQRPIKRLKGFQRVTIPVGQTKTVNIEVDCADLWFWDDKND
                     RITFDQGKYIFEIGASSRDIKGQVEAVMSGQINLGLKTVVAQSDQIVLRAGNATSTQV
                     TASLTDDSFIDLAKAKVTYKSNNPVVASVDAAGKVTALRPGVATIFAEVSYNGTTASD
                     NYSIKVMPDLTPASIKVNGKPVIGFNASTKAYSYLLKAGSKLPVVEAKALSSDIAVNI
                     EQAKKIPGTAVVTYIDNNTFEKNTYYINFDVASVSDEFDNGQLGKQWEWVRENKANYK
                     LTSPQGALTITTEKGDISEGSNNASNLLLQSANNDWTVDVKLNCSRTPSQPENAGIVA
                     YQDDNNFVKLMFRAVIKT"
BASE COUNT          683 a          597 c          559 g          853 t
ORIGIN      
        1 taggcgaatt ggctttttcg cttaagagga gtttcccgtt ggcatcgtaa aaactaagct
       61 tatctgtttt cttatccaac tcaaccacaa tatttttcaa cgtagtgttg atcgactttt
      121 tcgattcaat tacgttgaac tcgggtactg acggtttgct ttcgaacacc aactcgggaa
      181 gagtttgatt ttcagcagta aaaaattgta cccggacagt attgtcggta aggggataaa
      241 tggcaagggt tcccttttct gtttttaaaa gtacccgatt gctctctttt acaaagttat
      301 cagcagcctg tagctgcaag ctcagtaaaa gagcaatgat ggccattttc agtattttca
      361 ttgtatttct attagttaga gtttcatttc agttatttct tcacgttgtc tcgatgtact
      421 aaaaggaaca atggtgaagc tgtaattcaa tccttcggca cttggccttt gtgccattca
      481 atgaagcaaa catacatgaa gcttctgggc aacctttcat tgccagttct ttactttcgt
      541 aacttacaaa taaagctcgc ggccattcct tgtttattcc ctgaatggaa gagttttcca
      601 acccgggatg aggagtgttg tcctgagcct gaatagtcag gcccaggaat actcccaaaa
      661 ttgttaattg taatctatga atcaatattt tatcaaagtt catagttgtc agttattgta
      721 tttctgtcat tgagtttctt atcaatttct acttgagtcc actattttga atcctgaaat
      781 aatcaaacga tacatcgaat ggagttgcag gttttgtagt gtcggaatta aaccaatacg
      841 tgctggtcat actttgagtg atgatgccgt cgcaagcaat caacccggct ttgatgtcct
      901 tcagcgcgat gtctgaagca cccagttttt caaatgtctt gccatcggtt gaataaaaag
      961 cagtgtaggt gctaccggct tttaccaatc gcaacaacaa gtttttatcc ccaatcactt
     1021 cttctttaag gttgactgac gcgattgatt tagcaatgcc gttttcttca agcaaaaggt
     1081 caacagtacc cggttgaaca ccgcgttgtc tcaagttttg atcactgcac ggaacatcag
     1141 tttcacgaaa ttattatcgt cctgataagc aacgattccc gcattttcgg gctgcgaagg
     1201 agtccttgaa caattcagtt tcacatcaac tgtccaatcg ttgtttgcgc tttgcaacag
     1261 caagttactg gcgttattgc ttccttcgga gatgtcgcct ttttcagtag ttatggtaag
     1321 ggcaccttgc ggcgaagtaa gtttatagtt ggctttgttc tcgcgaaccc attcccactg
     1381 ttttcctaac tgtccgttgt cgaattcgtc gcttaccgaa gccacatcaa aattgatata
     1441 gtaggtattc ttctcaaaag tattgttatc gatgtaagtt acgacagccg ttccaggaat
     1501 tttcttcgct tgttcgatgt taacagcaat gtcgctgctc aatgctttgg cctcaaccac
     1561 aggtaattta gagcctgctt ttaacaaata gctgtatgcc ttggttgatg cgttaaatcc
     1621 aatgacaggt tttccgttta ctttgatgct ggcaggagta agatcgggca ttacttttat
     1681 agaatagtta tcggaagccg ttgttccatt gtagcttacc tcggcaaaga ttgtggccac
     1741 acccggacgg agcgcggtaa cttttccagc agcatccaca ctggctacca ctggattatt
     1801 gcttttgtaa gtaacttttg ctttagctaa atcgatgaag ctatcgtcgg taaggcttgc
     1861 agttacctga gttgatgtag cattgcccgc acgaagtaca atttgatcag attgggctac
     1921 cacagttttc aatccaaggt taatctgtcc gctcattacg gcttcaacct gtcctttaat
     1981 gtcgcgtgac gaagcaccga tttcaaagat gtattttccc tgatcgaaag tgatccgatc
     2041 atttttgtcg tcccagaacc aaaggtcggc acaatccact tcaatgttaa ctgttttggt
     2101 ttgtccgaca ggaatggtca cccgttggaa acctttcaac cgtttgatag gacgttgcag
     2161 tgaagcagca ctttcgggcg tttttacata aatctgaact acttcgtcgc catctacatc
     2221 gcctgtattt ttcacgtcaa cgctaacagt tactttgtcg ttaggtgaaa ttttgctttt
     2281 actgatgctg aaattgctga attcgaaagt ggtatatgac aacccaaaac caaattcata
     2341 agaaacatct ttgttgaaat accaatatgt gcgaccgttt ttgccatcgg cagcacgcaa
     2401 gttatagtca ttgaaatcgg gaaggtcttt caccgatttg taccaggtta cgctggtttt
     2461 tccaccagga ttcacttcac caaaaagcac tttagccatt gcagcacctt gtgcctgacc
     2521 attgtagccg gtgtagataa taccaggaat atttacatta ttctttatgt cttcgacttc
     2581 gaccataccc atgcgcttgc gctcggcggc ggggacacgg cggatgatct ccgccccctc
     2641 tgccggcaca cctgccacaa ggcgaagacg cggacggata tcgcggcaat cg
//
LOCUS       DAOPUW010000242         2691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_39502_length_2691_cov_4.182853, whole genome shotgun sequence.
ACCESSION   DAOPUW010000242 DAOPUW010000000
VERSION     DAOPUW010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39502_length_2691_cov_4.182853"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PKJ43_05025"
     CDS             complement(<1..555)
                     /locus_tag="PKJ43_05025"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNZ71962.1"
                     /translation="MNSNHPIEKTPTPNIKNIILVASGKGGVGKSTVAAGLALSLSLE
                     GYSVGLFDADIYGPSIPTLFNLINEYPVMTDHGGKPRIEPFEKLGIKVMSIGFLINNH
                     QPLLWRGPMASTSLKQLFNDTDWGNLDYLIIDTPPGTGDIHLTLLQQYNVAGAIVVTT
                     PQNMALTDVQKAIGMFFNEHVGVPV"
     gene            complement(580..1524)
                     /locus_tag="PKJ43_05030"
     CDS             complement(580..1524)
                     /locus_tag="PKJ43_05030"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ71963.1"
                     /translation="MICYGPVPSRRLGRSLGINNIPSPKMCTYSCIYCQIGVTNKFCN
                     TPQPFYEPDIIFSQVKKHLEKLSPTDRPDYLTFVSNGEPTLDSNLGKTIQLLKKLGFP
                     VAVITNGSLLNLKEVRRNLKNADLVSVKVDANDEEVWKSINLPGEGIFFENYIEGILK
                     FSRKFKGILTTETMLVEGVNDDTKILSRTANLISAIHPVKAYLSIPIRPPAISAVKVP
                     SETTINKAYQIFHWKIDQTELMLGFEGTGLASTGNPIEDITNICAVHPIREDSLQELL
                     GKYKGGSDILEILLHGNHIEKVKYQSQTFYIRKFHNSL"
     gene            complement(1529..2392)
                     /locus_tag="PKJ43_05035"
     CDS             complement(1529..2392)
                     /locus_tag="PKJ43_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ71964.1"
                     /translation="MNEIVVLSGKGGTGKTMLSAAFATLEKNVVVADCDVDAANLHLI
                     LQPKNEIEEVFVTGHRAQIDYNKCTNCGLCVEHCRFDAISIVDGMVKISETSCDGCKL
                     CSRVCPIGAIEMVPSDKSRWYAGNYRNGKMVHARLAPGEENSGKLVNVVRDYAKKIAV
                     ETNAKTIIIDGPPGTGCPVVSSLTGTKKVVLVTEPSESGFHDLKRIVELSESFHIKPS
                     VIINKYDLNYEMTRKIEKWCSNFKIPVIGKILFDKQIVEAMLHCKSIVEWKPSLEASE
                     EIKNIWKIINC"
     gene            complement(2389..>2691)
                     /locus_tag="PKJ43_05040"
     CDS             complement(2389..>2691)
                     /locus_tag="PKJ43_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase"
                     /protein_id="HNZ71965.1"
                     /translation="DYVVLVTEPTPFGLNDLKLSAETLDEIGKPYGVVVNRAGLGNND
                     VYLWLKEKKIPLLMEIPFDKEIAMVYSEGKLIAELNSKYSDKFMQLLQSVKKELKR"
BASE COUNT          711 a          584 c          534 g          862 t
ORIGIN      
        1 cacaggcacc ccaacatgct cgttgaaaaa catcccaatg gctttttgca catcggtgag
       61 ggccatgttt tgcggggttg ttaccacaat tgccccggca acattgtatt gttggagcag
      121 cgtaaggtgg atgtcgcctg ttccgggagg cgtatcaatt atcaggtaat cgaggttgcc
      181 ccaatcggtg tcgttaaaaa gttgttttaa acttgttgaa gccattggcc cgcgccatag
      241 caggggctgg tggttgttga taagaaatcc tattgacatg actttaattc ccagtttttc
      301 aaaaggttcg atgcgcggtt taccgccatg atcggtcatt accggatatt cgtttatcag
      361 attgaacaag gtgggaatag acggaccgta aatatcggca tcgaaaagcc ccacagaata
      421 gccttcaaga gacaacgaca aggccaaacc tgcagccacg gtcgattttc caacgccccc
      481 tttgcccgaa gctactaaaa tgatgttttt gatattggga gttggtgtct tctctatcgg
      541 gtgatttgaa ttcattttat ccatttttaa tgcgtttttt cataacgagt tatgaaattt
      601 tcgaatgtaa aacgtttgtg attgatattt aactttttct atatgattgc catggagcag
      661 tatttccaaa atatcggagc cacctttgta tttaccaagc agctcctgca aagaatcttc
      721 cctgatcgga tgaacggcac aaatgtttgt aatatcttca attggatttc cggtcgatgc
      781 caaaccggtt ccctcaaagc ctaacatcag ttcggtttgg tcaatcttcc agtgaaaaat
      841 ttgatatgct ttattaatag tggtttcaga aggaactttc actgccgaaa tagctggtgg
      901 cctgattggt attgataagt aagctttcac tggatgtatg gctgaaatca agtttgctgt
      961 tcttgaaaga atctttgtat cgtcatttac tccttcaaca agcattgttt cagttgtaag
     1021 tatccctttg aattttcttg aaaactttag aattccttca atataatttt caaaaaaaat
     1081 accctcaccg ggaagattga tgcttttcca cacctcttca tcattggcat caacttttac
     1141 cgaaaccaaa tcggcattct tcagatttct tctaacttcc tttagattca atagagaacc
     1201 attggtaata actgcaaccg ggaatccaag ttttttaagt agttgaattg tttttcccag
     1261 attggagtca agggttggct ctccatttga gacaaaggtc aaatagtcag gccggtcagt
     1321 tggtgatagc ttttcaaggt gtttttttac ttgcgaaaat ataatatcag gttcataaaa
     1381 tggctgagga gtattgcaga atttatttgt aacacctatc tggcaataaa tacaagaata
     1441 ggtacacatt tttggtgatg gaatgttgtt tatccctaaa cttctaccta accgacgtga
     1501 aggaactggt ccgtaacaaa tcatagattc agcaattaat tatcttccaa atgtttttga
     1561 tctcttctga tgcttctaaa cttggtttcc attcaacaat gctcttacaa tgaagcattg
     1621 cttcaacaat ttgcttgtcg aaaagaattt tccctataac cggtatttta aaattgctgc
     1681 accatttttc aatttttcgt gtcatttcgt agttgaggtc gtatttgttt attattaccg
     1741 atggcttaat gtggaaactt tccgatagct ctacaatccg tttcaaatcg tggaaacctg
     1801 attccgatgg ttcggtaaca agtaccacct tttttgtgcc cgttaacgac gaaaccacag
     1861 ggcaccccgt gcctggaggc ccatcgatga tgatggtttt tgcattggtt tcgacagcaa
     1921 ttttttttgc gtaatcgcgc accacattga ccaatttccc cgagttttcc tcgccaggag
     1981 ccagtcgtgc atgtaccatt ttaccgtttc ggtagttgcc cgcataccac cggcttttgt
     2041 cgcttggcac catttcaatt gcaccaatcg ggcaaacacg cgaacagagc ttgcagccgt
     2101 cgcacgatgt ttcggatatt ttcaccatac catcgacgat tgaaatggca tcgaaacggc
     2161 aatgttcgac acaaagcccg caattggtgc atttgttgta gtctatttga gcacggtgac
     2221 ctgttacaaa aacctcctca atttcatttt tgggttgaag tatcagatgc aaattggcgg
     2281 catcaacatc gcaatcggcc acaaccacat tcttttcaag tgttgcaaaa gcagcgctta
     2341 acatggtttt gcctgttccc cctttgccgc tcaacaccac aatttcgttc atcgtttaag
     2401 ttctttttta acagattgaa gcaactgcat aaatttgtcg gagtattttg aattcagttc
     2461 agcaatcagc ttcccctctg aataaaccat tgcaatctcc ttgtcaaaag gaatctccat
     2521 taacaagggt atcttttttt cttttaacca caaatagaca tcgttatttc ctagccctgc
     2581 ccggttgact accactccgt agggttttcc aatttcgtcg agcgtttcgg ctgaaagctt
     2641 caggtcgttt aatccgaatg gtgtgggctc ggtcaccaaa accacatagt c
//
LOCUS       DAOPUW010000243         2684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_39649_length_2684_cov_2.289464, whole genome shotgun sequence.
ACCESSION   DAOPUW010000243 DAOPUW010000000
VERSION     DAOPUW010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39649_length_2684_cov_2.289464"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /gene="nadA"
                     /locus_tag="PKJ43_05045"
     CDS             complement(<1..444)
                     /gene="nadA"
                     /locus_tag="PKJ43_05045"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF014498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNZ71966.1"
                     /translation="MASNTNTADQLKRDGYIDEQIPAGINLIDEINRMKKEKNAVILG
                     HYYISPELQDISDFVGDSLALAQEAARTTAEIIVFVGVHFMAETAKIINPTKKVLLPD
                     LKAGCSLAESAPIDSFREFKKKYPNHKVISYINCTADLKTETDIVC"
     gene            741..2108
                     /locus_tag="PKJ43_05050"
     CDS             741..2108
                     /locus_tag="PKJ43_05050"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNZ71967.1"
                     /translation="MAIFDLSIHIFRRDLRLDDNSGLLAALEKSKCVLPVFIFDERQI
                     NSPYAGKNSLSFMLNSLVELDLALRKLGSRLYVFQGESHLVLQNLLQSTQAQAVFVNR
                     DYTPFSLERDQNMANICQGLGVGFFSFADALLTEPEQVTKDDGLPYTVFTPFMKKARK
                     IHIPVPVENTFNNYYNGSIEKAIDDFHSMFTDWERKTLLTGGREEGKKLLVGIDSLDN
                     YSETRNIPSVKGTSLLSAHHKFGTISVRETYHQILSVFDTEHSLINELYWRDFFTHVA
                     FHFPRVFGNVFNEKYANLAWENDETKFGHWCSGTTGFPIVDAGMRELVATGFMHNRVR
                     MVVASFLTKDLHIDWRWGERFFANHLIDYDPSVNNGNWQWAASTGCDAQPWFRIFNPW
                     SQQQKFDPDGVYCKKWIPELAKLSANDIDNLTGHITSKLHYPPPIVDHASQVTIAKAM
                     FSNLK"
     gene            2160..>2684
                     /locus_tag="PKJ43_05055"
     CDS             2160..>2684
                     /locus_tag="PKJ43_05055"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0044210 - 'de novo' CTP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine monophosphate kinase"
                     /protein_id="HNZ71968.1"
                     /translation="MTTPYKRVLLKLSGESLMGNVGYGIDPARLASYAGQIKEIVDMG
                     IQVAIVIGGGNIFRGLSGAAKGFDRVKGDQMGMLATVINSLALQSELEKNGVNAHTFS
                     AIRMEPIGEYYQKEKALASLGRGEVAIISAGTGNPFFTTDTASALRAIELGAHVMLKG
                     TRVDGIYTADPEKDP"
BASE COUNT          725 a          532 c          604 g          823 t
ORIGIN      
        1 gcatacgata tcggtttctg tttttaaatc ggccgtgcag ttaatataac tgattacctt
       61 gtggttgggg tatttcttct taaattccct gaagctgtca attggagccg attctgccag
      121 tgagcagcct gctttcaggt cggggagcaa cactttctta gtggggttga ttattttagc
      181 tgtttcagcc atgaagtgaa ccccaacaaa tacaataatt tcggcggtgg ttcgggccgc
      241 ttcctgggcc agggcaaggc tatcgcccac aaaatctgag atgtcctgaa gttctgggga
      301 tatgtagtaa tgtccaagta ttacagcatt tttttctttt ttcatccggt taatttcatc
      361 aatgaggttt atccctgccg gaatttgttc atcaatataa ccgtctctct tcaactggtc
      421 ggctgtgttg gtgttgcttg ccatgttttg taatattgaa attgagcaac aaaggtaaaa
      481 aaaaggcaat aactacgaag agtgaaaaag actaacatgt ctgagataaa caatccttaa
      541 tgatgccctt aacgcatggg ttgcaatgct tttttttatg ttttcacagg tgaacagata
      601 atgtgttgat aaataaaaga atgtatgctg attttctatt ttaatggagg agttttctgc
      661 ctttcaaaaa cttttttcac agctcatggt ttcaactata cttaaaataa tgtagtttgt
      721 tttaaagttt ttataaaagc atggcaattt ttgatctatc aatccatatt tttcggcgcg
      781 atttacgtct cgatgataat tcaggcttac ttgctgccct tgaaaaatcg aagtgtgtac
      841 ttcctgtttt tatcttcgac gaacggcaga ttaacagtcc gtatgccggg aaaaacagtc
      901 tttcgtttat gcttaattcg cttgttgagc ttgatttggc tttgcggaaa ttaggctcgc
      961 gtttatatgt tttccaggga gaatcacatc tagtattgca gaatttgttg cagtcgactc
     1021 aggctcaggc tgtttttgta aaccgcgatt atactccgtt cagcctcgaa cgtgaccaaa
     1081 atatggccaa catttgtcag ggcttgggcg ttggtttctt ttcttttgcc gatgctcttc
     1141 ttactgaacc tgagcaggta acgaaagacg atggtttacc ttatactgtt tttaccccgt
     1201 tcatgaaaaa ggcccggaaa atacacatcc ctgttcctgt tgaaaataca ttcaataatt
     1261 actataacgg gtcaatagaa aaagccattg atgattttca ttctatgttt accgattggg
     1321 aacgtaaaac attgcttact ggaggacgag aggaagggaa aaaattattg gttggtattg
     1381 attctctcga taattacagc gaaacgcgca atattccttc ggtgaaggga accagtttgc
     1441 tttcggcaca ccataagttt ggaaccattt cggtgcgtga aacctatcat caaattcttt
     1501 cagttttcga tactgaacac tcattaatta atgagctcta ctggcgcgat ttctttactc
     1561 acgtggcttt ccattttcca cgagttttcg gaaacgtttt taatgaaaag tatgcgaacc
     1621 ttgcctggga aaacgacgag acaaagtttg gccactggtg tagcggaact accgggtttc
     1681 ccattgtcga tgccgggatg cgcgaacttg ttgctacggg gttcatgcac aatagagtac
     1741 gtatggttgt cgcttctttt ctaactaaag atttgcacat tgactggcga tggggcgaac
     1801 gtttttttgc caatcatctt atcgactacg atccctcggt gaataacggg aactggcaat
     1861 gggctgcttc aaccggctgc gatgcccaac cttggttccg gatttttaac ccatggagcc
     1921 agcaacaaaa gttcgatccc gatggtgttt attgcaagaa atggattcct gagctagcaa
     1981 aattatcggc aaacgatatt gataatctta ctgggcatat aacgagcaag ttacattatc
     2041 cacccccgat tgtagaccat gcttcgcaag taactattgc taaggcaatg ttcagcaatt
     2101 tgaaataaac agggtcaata aattctgaaa atactacctt tgtcgaaaaa aatagtaata
     2161 tgacaactcc ttataaacga gtactactaa agctttcggg tgaatcgttg atgggcaatg
     2221 tggggtatgg aatagacccc gcgcggcttg cctcgtatgc tggtcagatt aaagaaatcg
     2281 tggatatggg cattcaggtg gcgattgtaa ttggcggtgg aaacattttc aggggcttaa
     2341 gtggtgcggc caaagggttc gaccgcgtta aaggcgatca gatgggaatg cttgctacag
     2401 ttatcaatag cctcgcgtta caatcagaac ttgaaaaaaa tggcgttaat gcgcacacat
     2461 tctcggccat acgaatggaa cctatcggag agtattatca aaaggaaaag gcattagcat
     2521 cgctcggaag gggagaagtg gccataattt ctgccggtac cggaaacccg tttttcacca
     2581 ccgatactgc ctcggctttg cgtgctattg agcttggtgc ccatgttatg ctcaaaggga
     2641 cccgcgttga tggcatttat actgccgacc ctgaaaaaga tcct
//
LOCUS       DAOPUW010000244         2675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_39817_length_2675_cov_3.300000, whole genome shotgun sequence.
ACCESSION   DAOPUW010000244 DAOPUW010000000
VERSION     DAOPUW010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39817_length_2675_cov_3.300000"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PKJ43_05060"
     CDS             <1..496
                     /locus_tag="PKJ43_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNZ71969.1"
                     /translation="KFPRSERDRMAQKMRDLVAKMKLIPNNALIKDKVGVFLDDSIVR
                     GTQLKDNVIDLHDAGIKEVHMRIACPPLIYPCEYLNFSRSRTSLELATRKAILKLEGK
                     EDANLEKYADPTTEEYARMVEQIRIDLGLTSLKFQKLDDLVEAIGLPKEKLCTHCWDG
                     SSSF"
     gene            616..2445
                     /locus_tag="PKJ43_05065"
     CDS             616..2445
                     /locus_tag="PKJ43_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNZ71970.1"
                     /translation="MKQAIILSLIISAVLSGQIRAQEPQIISVQQRNITSLNGTWKYI
                     LDQYENGYYNYRYEPMDQQAFNPRSAEAIFYDYHPTDKTDRVEYDFDKSEELQVPGSW
                     NYQDDKLFYYEGTIWYRKKFDYTLSSSNRLFLYFGAINYEAHIYLNGKKLGMHVGGFT
                     PFNFEITSMLKEKDNSLVIKVDNKRKREGVPTLNTDWFNYGGITRDVLLIEVSSTFVR
                     DYRIQLTKNSLSKVEGYVNLDGANRKGKVTVEIPELKINQQFTIDSNGHAPISFTLNN
                     AILWSPENPKLYEVKIRFGESVITDKIGFRSIETKGSDLLLNGKPIFLRGISIHEENG
                     SRGDRAWSIEDAQMILSRAKELNCNFVRLAHYPHNENMIRVAEQLGLLVWSEIPVYWT
                     IDWNNESTYKNAENQLKDMITRDMNRANIIVWSVANETPVSDARTRFLTNLINKTREL
                     DNTRLVSAALEKHTLSNTPPHFAVEDPFAQYVDILSVNQYMGWYDGLPEKCSRSEWTI
                     KFNKPMIISEFGGGAKYGYHADKDTRWSEEFQEELYIQNIKMLEKIPQLRGMTPWILN
                     DFRSPRRLLPGIQDGWNRKGLFSEKGEKKKAFYILQEYYSKKQ"
BASE COUNT          884 a          519 c          533 g          739 t
ORIGIN      
        1 gaaatttccc cgcagcgagc gggatcgcat ggcccagaaa atgcgcgacc tggttgcaaa
       61 aatgaaactg atccccaaca atgccctaat taaagataaa gttggggttt tcctcgacga
      121 ttcaattgta cgagggactc agctaaaaga caatgtaatc gatcttcatg acgcagggat
      181 taaagaagtg catatgagaa tagcttgtcc gcctcttatt tatccatgtg aataccttaa
      241 tttcagccgt tcgcgtactt cgcttgagtt ggctacacga aaagcaatat tgaaattaga
      301 agggaaagaa gatgctaacc ttgaaaaata tgctgacccg actacagaag aatatgcccg
      361 catggttgaa caaattcgca ttgatttagg gcttacatcg ttgaaatttc agaaacttga
      421 cgatttagtc gaagcaattg gattgcccaa agaaaagctt tgcacgcatt gctgggacgg
      481 ttcgagctcg ttctagtcca ttttctgcct gttttttgct attttagctt cctataatac
      541 ccgttgaact tcgtgaggat atataagttt tcacgatgtt taactattgt taaatcagca
      601 ctataacgat acaatatgaa acaggcaatt attctctcat tgattatttc ggccgtatta
      661 tctggacaaa tcagggcgca agaaccacaa ataatttcgg ttcagcaacg aaatattact
      721 tcattgaacg gaacatggaa atatattctt gatcaatacg aaaacgggta ttataattac
      781 cgttatgaac ccatggatca gcaagcattt aaccctcgtt ctgcagaagc aattttctac
      841 gattatcatc cgacagataa aactgatcgt gtagaatatg atttcgacaa atctgaggag
      901 cttcaagtcc ctggttcctg gaattatcaa gacgataaac ttttctatta cgaaggaacc
      961 atatggtacc gtaaaaaatt tgattatacc ctctcctctt ccaatcgact tttcctgtat
     1021 tttggagcta tcaactacga agcacatatt tacctgaatg ggaagaagtt gggaatgcat
     1081 gtcggtggat ttaccccttt caatttcgaa ataacttcga tgttgaagga aaaggataac
     1141 tctctggtta ttaaagtaga caataaacga aaacgcgaag gtgtacctac attaaacacc
     1201 gactggttca attatggagg gataacacgt gatgtgcttt taattgaagt ttcttcgact
     1261 tttgtgcgcg attaccgtat tcagcttaca aaaaattccc tttcaaaggt cgaaggatat
     1321 gtaaaccttg atggagccaa tcgcaaagga aaagttactg ttgaaatccc cgaattaaaa
     1381 ataaatcaac aatttactat tgacagtaac ggccacgcac caatttcatt cacattaaat
     1441 aatgcgattt tatggagccc tgaaaaccct aaattatacg aagtgaaaat tcgttttgga
     1501 gaatcggtta ttacagacaa aattggattc agatcaattg aaactaaagg atctgattta
     1561 ctgttgaacg gcaaacctat ttttttgagg ggaataagta ttcacgaaga aaatggctca
     1621 cgaggcgaca gagcatggtc gatcgaagat gcacaaatga ttctatcaag agctaaagaa
     1681 ttgaattgta attttgtacg acttgcccat tatccacata atgaaaatat gatacgagta
     1741 gccgaacagc taggtttgct ggtttggtca gagattccgg tatactggac aattgactgg
     1801 aacaatgaat cgacctataa aaacgctgaa aatcaactta aagatatgat cacccgtgac
     1861 atgaaccggg ccaatattat tgtttggtcg gtagctaacg aaacccctgt aagcgatgcc
     1921 cgcacaaggt ttttgaccaa tcttattaat aaaacacggg agctggataa tacacgcctt
     1981 gtaagtgcag cacttgaaaa gcatacactc tccaatactc caccacattt tgcagttgaa
     2041 gatccctttg ctcaatatgt tgatattctt tctgttaatc aatatatggg ttggtatgat
     2101 ggattgcctg aaaaatgtag ccgttccgaa tggaccatca aattcaacaa accaatgatt
     2161 atcagtgaat ttggtggtgg cgcgaaatat ggctatcatg cagacaaaga cactcgctgg
     2221 agcgaagaat ttcaagaaga actttacatc cagaatatta aaatgcttga aaaaatacct
     2281 caactcagag gaatgacccc ttggattctc aacgacttcc gttcgccccg caggttattg
     2341 cccggaatac aggacggatg gaaccgtaaa ggccttttct cggaaaaagg tgaaaagaag
     2401 aaggcatttt acattttaca ggaatattac agcaaaaaac aataagagtt acagaactaa
     2461 ggaataatgc aacgctattc actgcggcat taatcaccat ataacaaaca aatataatga
     2521 gagttaacaa taaacaaacg atcgaacttt tatcccctgt agggaatatt gaatcgtttt
     2581 atgcagctct cgatggaggg gccgatgcga tttacctcgg actgaagaat ttcaacgcgc
     2641 gtaaccgggc aatgaacttt accgcctggc aagtt
//
LOCUS       DAOPUW010000245         2668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_39979_length_2668_cov_2.011481, whole genome shotgun sequence.
ACCESSION   DAOPUW010000245 DAOPUW010000000
VERSION     DAOPUW010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39979_length_2668_cov_2.011481"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PKJ43_05070"
     CDS             <1..658
                     /locus_tag="PKJ43_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNZ71971.1"
                     /translation="AAKVIENSQRDINIAFVNELSKIFNRMGIDTHAVLEAAGTKWNF
                     LPFKPGLVGGHCIGVDPYYLAQKAQEAGYHPEIILAGRRLNDSMGAYVASEVVKLMIK
                     NGTPVKGAKALMLGITFKENCPDIRNTKAIDIYHELCEYGIEVDVYDPWAKPEEVMHE
                     YGIKSITEYPEGNGYGAIILAVAHNEFKTLNYKAHKEAGAVIYDVKGILEKELTDGRL
                     "
     gene            645..1994
                     /locus_tag="PKJ43_05075"
     CDS             645..1994
                     /locus_tag="PKJ43_05075"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HNZ71972.1"
                     /translation="MEDFSRMVDVKSAIELGEGQFIEFKEAADKNLAREMVAFANSSG
                     GFIYLGITDTCNIKGVETSNKLKSQIQDTARNCDPPLAILLLQQENVLIIEVKEGVSK
                     PYSCASGFYMRMGANSQKMSRDEILSLAIKTGKIRYDEQICAQFDWRDFDEEKFEYYL
                     KLARISNNLPKEEILRNLRVLTSDGMTNAGVLYFAKQPYKYIISSKIRCIHFNDDERV
                     DILDKKEVDRGIIGNIEYAITYLKELVPIRFEIKDLKRDEYPEYPIEAYREAIVNAII
                     HFDYFLGDTIAIEKLKSRIIINNKGELLFPKSEFGKKSEARNRLLVDLLARTDFMEKA
                     GTGIKRVTNACIENGNKFNFEFSDSFWITIETNNTEKVVEKVVEKVVEKVVENLSDKQ
                     KNIIELLNKNPYFSSRELAPLVGISQRKIQENIAKLKEQGIIERIGPAKGGHWKINR"
     gene            complement(2149..2319)
                     /locus_tag="PKJ43_05080"
     CDS             complement(2149..2319)
                     /locus_tag="PKJ43_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71973.1"
                     /translation="MIPYSCITSSGEVPFKFPSGFHGVDVDGLGVAYPEASSGLRFAC
                     VGAVPGEICAAF"
BASE COUNT          883 a          550 c          578 g          657 t
ORIGIN      
        1 agctgccaag gtaattgaaa actcgcagcg cgatattaac attgcctttg taaacgagct
       61 gtccaaaata tttaaccgta tgggcatcga tacacatgct gtattggaag ctgccggcac
      121 caagtggaac ttcctgccct ttaaacccgg gctggtgggc ggacactgca ttggtgttga
      181 cccttattat ttagcacaga aagcacagga ggcgggttac caccccgaaa ttatattggc
      241 aggccgccgc ctcaacgact ccatgggggc atacgtagcc tccgaagtgg taaagctcat
      301 gattaaaaat ggcacacctg taaaaggagc aaaagcattg atgctgggca ttacctttaa
      361 ggaaaactgc cccgacatac gcaacaccaa agcgatagac atttaccacg aattgtgcga
      421 atacggcatc gaggttgatg tgtacgaccc ctgggcaaaa cctgaagagg taatgcatga
      481 atacggcatt aaatcgataa ctgaataccc ggaaggaaat gggtatgggg cgattatttt
      541 ggctgttgcc cacaacgaat ttaagacact taattataaa gcccacaaag aagccggcgc
      601 agtgatttac gatgtaaagg ggatactgga gaaggaattg acggatggaa gactttagca
      661 ggatggtaga tgttaagtct gcgatagaac tgggcgaagg ccaatttatt gagttcaaag
      721 aagcagcaga caaaaacctt gcaagagaga tggttgcatt tgccaactct tcagggggtt
      781 ttatttacct tggaataacc gatacctgca atattaaagg tgttgaaaca tcaaacaaat
      841 taaaatctca aatacaggat acagcccgaa attgcgaccc tccgctggca atcttgttgt
      901 tgcaacaaga gaatgtactt ataattgaag ttaaagaagg ggtaagcaaa ccctattcct
      961 gtgcttcggg tttttatatg cgaatgggag caaactccca gaaaatgagc agagatgaga
     1021 tattatcact ggccattaaa acgggtaaaa tccgctacga cgaacagatt tgcgcccagt
     1081 tcgactggag ggacttcgat gaagagaagt ttgagtatta tctcaaattg gcccgaatct
     1141 caaacaattt gcccaaagaa gaaattttga gaaatctgcg cgtactaaca agcgatggca
     1201 tgaccaatgc cggagttctc tactttgcaa aacaaccata caaatacatc atctcttcaa
     1261 aaatcaggtg catccatttc aacgacgacg agcgtgttga tatcttggat aaaaaggagg
     1321 ttgatcgtgg tataattgga aacatcgaat atgccattac ataccttaaa gagcttgtac
     1381 cgatccgttt tgaaattaaa gatttaaaac gagatgaata cccggaatat cccatcgaag
     1441 cataccgcga ggctatagtt aatgcgataa tccatttcga ttactttcta ggtgacacta
     1501 tcgccattga aaaacttaaa tccaggatta ttatcaacaa taaaggcgaa ctgcttttcc
     1561 caaaaagcga atttgggaaa aagagcgagg cccgaaaccg tttattggta gacctactgg
     1621 ctcgtaccga ttttatggaa aaagctggaa caggcataaa acgggtaacg aacgcttgta
     1681 tcgaaaatgg caacaaattt aatttcgaat tctccgattc gttttggata acaattgaga
     1741 ccaataatac ggaaaaggtc gtagaaaagg tcgtagaaaa ggtcgtagaa aaggtcgtag
     1801 aaaatttatc ggataaacag aaaaatatta tagaattatt aaacaaaaat ccatattttt
     1861 cttcacgaga acttgcacct ttagtgggaa tatctcaaag aaaaattcaa gaaaatattg
     1921 ctaaactaaa agaacagggt ataattgaac gcattggtcc cgcaaaagga ggtcactgga
     1981 aaattaacag gtaggtcaat gaatttaaac aatatacccg ttaaaggggc tcgcgccctc
     2041 atgctgggca ttacctttaa agaaatttac cccgtgaaat tgcaaaccag ttattttatt
     2101 gaaaattacc caaaaaattg cgaatcccat ttctatgaag aatacccctc aaaatgcagc
     2161 gcatatttca ccggggactg ccccgacaca agcgaagcga agtcccgaac tcgcttcggg
     2221 atacgcaaca ccaaggccat cgacatctac cccatgaaat cccgaaggga atttaaacgg
     2281 gacctctccc gaagaagtaa tgcacgaata cggaatcacc tccaaaacta cacttgagca
     2341 aatcccctcc ttggaggggt cccgatagct atcgtggagg ggtgggttcc tccccggagg
     2401 gatcagggat aggttatagg gccatcatcg ttgccgtgtc ccataaccaa tttctttctc
     2461 tcgacctcaa agcccataaa aataacgggg cggtgattta cgatgtaaaa gggatactgg
     2521 cgaaagattt gaccaatgga agattataaa gtacaaaccc atgcactcat caatgaagta
     2581 tcaagacatt agatagtaga catttccgct tccgtactcc ggtctccttt ttacttttta
     2641 ctttttactt tttacttttt acttattt
//
LOCUS       DAOPUW010000246         2667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_40002_length_2667_cov_2.812404, whole genome shotgun sequence.
ACCESSION   DAOPUW010000246 DAOPUW010000000
VERSION     DAOPUW010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40002_length_2667_cov_2.812404"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1286
                     /locus_tag="PKJ43_05085"
     CDS             132..1286
                     /locus_tag="PKJ43_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNZ71974.1"
                     /translation="MAEFFSFIGENISQFIHFTAFANFTIGHLIMICFGLLFIYLAIV
                     KEFEPMLLVPIGFGILIGNIAFSPGFEIGIYEEGSVLNYLYFGVRYGVYPPLIFLGIG
                     AMTDFSALISNPKLMLVGAAAQLGIFGAYCLALALGFTPNEAGAIGIIGGADGPTAIF
                     LTSKLAPDLLGAIAISAYSYMAMVPVIQPPIMRLLTNSKERSIRMKPPRAVSKKEKVI
                     FAIAGMIFTTSIVPSAIPLLGMLFFGNLLKESGVTRRLADTAKGPMIDIVTILIGLTV
                     GASTQATTFLTWKSVGIFFLGALSFAIATFGGVIFVKFFNLFLKEGNKINPLIGNAGV
                     SAVPHSARVSQVVGLQHDKTNHLLMHAMGPNVAGVIGSAVAAGILLSFLS"
     gene            1811..>2667
                     /locus_tag="PKJ43_05090"
     CDS             1811..>2667
                     /locus_tag="PKJ43_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNZ71975.1"
                     /translation="MTMEEKVKELLDLRTQARKGGGQKRIDKQHSQNKLTARERINLL
                     LDEGSFEEFDMFVTHRCTNFGLENESYLSDGVVTGYGTIDGRTVYVFAQDFTIFGGSL
                     SESFAKKICKIMDIAMKVGAPVIGINDSGGARIQEGVNSLAGYAEIFQRNIMASGVIP
                     QISAIFGPCAGGAVYSPALTDFILMSKQSSYMFLTGPKVVKSVTGEDVTQDQLGGAQV
                     HATKSSVAHFTSETEEEGIMKIRKLFSYLPSNNMEEAPMVECNDSIHRMEDRLNTIIP
                     DNANKPYDV"
BASE COUNT          802 a          526 c          534 g          805 t
ORIGIN      
        1 gttgcttgaa gcaatgaaaa tggagaataa cattgaagcc gacaaaactg gaaccataat
       61 ctctattgaa aagcaaaagg gcgacaatgt tatggaaggt gatgttttgc taataatagg
      121 agaataaaaa catggcagaa ttttttagtt ttataggtga aaacatatcg cagtttattc
      181 attttactgc atttgccaac ttcacaattg gtcaccttat catgatttgc tttggcttgt
      241 tgttcatata ccttgccata gtaaaagaat ttgagcctat gttattggtt ccaattgggt
      301 ttggtattct cattggaaat attgcatttt cgcctggttt cgagatcggg atttatgaag
      361 aaggcagtgt tctaaattat ctctattttg gtgtaaggta tggagtttat ccgcctctta
      421 ttttcttagg cattggtgca atgaccgact tttcggcgct aatctctaat cctaaattaa
      481 tgctggttgg agcagcagcc cagctcggta tctttggggc atattgcttg gcattggcct
      541 taggttttac ccccaatgaa gccggagcta tcggtattat tggtggtgcc gacggaccaa
      601 cagccatttt cctcacatcg aaacttgcac ctgacctact cggtgccata gccatttcag
      661 cttactcgta tatggccatg gttccagtta tccaacctcc gattatgcga ttgcttacaa
      721 acagcaaaga acgctctatt cggatgaaac caccacgcgc tgtgtctaaa aaagaaaagg
      781 taatatttgc cattgccggt atgattttta caacgtcaat cgttccgagc gctataccat
      841 tacttggaat gcttttcttt ggaaatctac taaaagaaag tggggtaacc cgccgacttg
      901 cagatacagc caaaggtcct atgattgata ttgttaccat tttaattgga cttactgtag
      961 gagcatcgac acaggcaaca accttcttaa cctggaaatc ggtaggtata tttttcttgg
     1021 gagctctttc gtttgcgata gcaacatttg gaggagttat ctttgtgaaa ttcttcaatt
     1081 tattcctcaa agaaggaaat aaaatcaacc cactcattgg aaatgcagga gtttcggctg
     1141 tgccacatag tgctcgcgtt tcgcaagttg taggacttca gcacgataaa acaaatcact
     1201 tattgatgca tgccatgggg ccaaatgttg ctggagttat tgggtcagct gttgctgctg
     1261 gtatacttct cagctttctt tcgtaatata tgaatattat tcagaaaagg cgcaattttt
     1321 gcgccttttt ttgtggcgta tatcactgaa tactatcata ttaaaatcat aaatgctgtt
     1381 ttgtttttaa tggttataaa caaggtaatt cccttgattt taaacaattt tctgttacac
     1441 cctatgtata aaattgttat gtttggagct taatttttga aagttagcct tagcagaaat
     1501 taatcgaaca aaccagcttt taataaagca cccatggttt ttcttgagat gatttattga
     1561 tgtacgtaga ttttattcaa gatgaacact ttattattta tcgaccataa aagattgaat
     1621 cattttaatc ttgcccaatc gataaccctg cccgaaaata attggacacg agtagtaaaa
     1681 agttatcccc acccttaccg tttcaacaac ctctatcaaa tcaaaacata agcatggaaa
     1741 ccatgtgtct acaagctttt caatccaatt ttgaaatcaa taaaaaaaat aatctaaaaa
     1801 tattcttatc atgacaatgg aggaaaaagt aaaagaatta cttgacctaa gaacacaagc
     1861 ccgcaaggga ggtggacaaa aacgaattga taaacaacac agtcagaaca aactgactgc
     1921 acgtgagcga atcaatttgc ttttagacga aggcagtttt gaagaatttg acatgtttgt
     1981 aacccaccgg tgcaccaact tcgggttaga aaatgaatcg tacctttcgg atggagttgt
     2041 taccgggtat ggaaccattg acggccgaac cgtttatgtt tttgcccaag actttaccat
     2101 ttttggcggt tcattatcag aatcgtttgc caagaaaatt tgtaaaatca tggatattgc
     2161 catgaaagtt ggcgctccgg taattggcat caacgactca ggcggggccc gaatacagga
     2221 aggcgttaac agccttgcgg gttatgccga aatttttcag cgcaacatta tggcatctgg
     2281 ggtaattcct caaatatcag ccatttttgg tccttgcgcc ggtggagcag tttactcccc
     2341 tgccctcacc gatttcattc ttatgtcgaa acaaagtagt tacatgttcc ttacaggacc
     2401 taaagtggta aaatctgtta ccggcgaaga cgtaactcaa gaccagttag gcggggcaca
     2461 agtacacgct actaaatcga gcgtagccca ttttacttct gaaacagaag aagaaggcat
     2521 tatgaaaatc cgcaagcttt tcagctacct gccttcaaac aatatggaag aagcacctat
     2581 ggttgaatgc aacgacagca tccaccgtat ggaagaccgg ctgaatacta taattcctga
     2641 caacgcgaac aaaccatacg atgtaaa
//
LOCUS       DAOPUW010000247         2666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_40019_length_2666_cov_3.307162, whole genome shotgun sequence.
ACCESSION   DAOPUW010000247 DAOPUW010000000
VERSION     DAOPUW010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40019_length_2666_cov_3.307162"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..907
                     /locus_tag="PKJ43_05095"
     CDS             8..907
                     /locus_tag="PKJ43_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ71976.1"
                     /translation="MENVNKNYSGFQALTDVSLSIDQGQVFGLLGPNGAGKTTLIRII
                     NQITAPDSGAVYFEGKLLGANDIYRIGYLPEERGLYKKMKVGEQALYLAQLKGLDRRT
                     ALKRLKFWFQKFEIESWWDKKLQELSKGMQQKVQFICTVLHEPKLLIFDEPFSGFDPI
                     NANLLKQEILSLRDKGATIIFSTHNMSSVEEICDHIALINKSKKILDGNINEVRASFK
                     TNTFDVVYRGEFATVEKQLNHGFEILSHKEDDWINSLTIKHSGGNNNELLQLITPHVE
                     IVSFSERIPSMNEVFIRVVENNK"
     gene            921..2273
                     /locus_tag="PKJ43_05100"
     CDS             921..2273
                     /locus_tag="PKJ43_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ71977.1"
                     /translation="MNKTLLIFKREYLTRVTKKTFLIMTLLMPILMGALMIVPSYLAN
                     KESTSEQLVGVVDRSSIFLGELENSKSTKFQFIKPEEYDKIKDNLQAKGFYALLEIPS
                     NILTTNKVMVSSQKQISIDVKSHIDSQLERVLENQKRAELISRVGIPDLEEQLKATKT
                     NISVETIKIGDDGLARKGSTEIAMGIGFAASFLIYMFVFMYGGMVMRGVSEEKQNRIV
                     EVIISSVKPIQLMMGKILAIAAVGLTQVLIWLVLLLGIFTGAKSMFGSDAEMLQLAQP
                     QAENIMMGNNSSVQMMQTIEPNQFDQIAEQIASLNLPMLIISFIVYFILGYLLYSSLM
                     AAIAAAVDSEEDVNQFMLPVTIPLIAAIVMMANVVKNPDGAIAIWGSHIPFTSPIIMM
                     VRIPFGVPWWELLISISVLAITMLGAVWVAAKIYRTGILMYGKKPTWKELGKWLKYKS
                     "
     gene            complement(2336..>2666)
                     /locus_tag="PKJ43_05105"
     CDS             complement(2336..>2666)
                     /locus_tag="PKJ43_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71978.1"
                     /translation="SFTKTIGSVEDGFFARIDVASNGATKFVELESTAWTTITTAEAL
                     KTAIDAATAIADGKWSKISASKSASYDYVLGVVYQDVYYMLHITNGKVESVTAGTKVT
                     INGQYKN"
BASE COUNT          817 a          470 c          587 g          792 t
ORIGIN      
        1 ttttagaatt gaaaatgtca ataaaaatta ttcgggattt caggcgctta ccgatgtgtc
       61 tctttcaatt gaccaggggc aagtgtttgg tcttttaggc ccgaacggtg caggaaaaac
      121 aaccttaatc cggatcataa accaaattac agcccccgat agtggagccg tatactttga
      181 aggaaagctg ctcggggcca acgatattta tcgtattggc tatttgcccg aagaaagagg
      241 cctttataag aaaatgaaag tgggtgaaca ggcattgtat cttgcccagc ttaaggggtt
      301 agacaggcga acagccttga aacgacttaa attttggttc caaaaatttg agattgaaag
      361 ctggtgggat aaaaagcttc aagagttgtc gaaagggatg cagcaaaaag tacaatttat
      421 ctgtactgtt ctgcatgaac caaagttgtt gatttttgac gaacctttca gtggtttcga
      481 cccaattaat gcgaatctgt tgaaacaaga gatattgagt ttgcgcgaca agggggctac
      541 cattattttt tcgacccaca atatgagttc tgttgaagaa atttgtgatc atattgcgtt
      601 gatcaacaaa tcgaaaaaga tactcgatgg caacatcaac gaagtgcgtg caagctttaa
      661 aaccaatact ttcgacgtag tttaccgggg agaatttgcc acggttgaaa aacagctgaa
      721 ccatgggttc gaaattcttt cgcataaaga agacgattgg atcaatagcc ttacaattaa
      781 gcattcgggg ggcaataata acgaattgct gcagctaatt accccacatg ttgaaattgt
      841 ctcattcagc gaacgcatac caagtatgaa tgaagtattt atcagagtag tcgaaaataa
      901 caagtaaaac cttaagcgaa atgaataaaa cattactcat attcaagaga gaatacctga
      961 cccgggtaac taaaaagacg ttcctgatca tgacattgct gatgccgata ctgatgggag
     1021 ccctaatgat tgttccatct tatcttgcaa acaaggaaag tacaagtgag caacttgttg
     1081 gagttgtcga ccggtcatct atatttcttg gggaacttga aaactctaaa tccacaaaat
     1141 tccaatttat taagcctgag gaatacgata aaataaaaga caatttacag gcaaaaggtt
     1201 tttatgccct tcttgaaata cccagcaata tccttactac caataaggta atggtatctt
     1261 ctcaaaagca gatttctatt gatgtgaaat cacacatcga ttcacaactg gagcgtgtac
     1321 tggagaacca aaagagagct gaattgattt cccgtgttgg tattcccgat ttggaggaac
     1381 agttaaaagc tacaaaaaca aatatctctg tcgaaactat taaaattggc gacgatggtc
     1441 tcgcccggaa aggctcaaca gaaattgcca tgggcattgg ttttgccgca agtttcctta
     1501 tttacatgtt tgttttcatg tatggaggga tggtaatgcg cggggtttca gaagaaaagc
     1561 agaaccgtat tgttgaggta attatttctt cagtgaagcc aattcaattg atgatgggga
     1621 aaattttggc aattgcagcc gttgggttaa cccaggttct catttggtta gtgttgttgt
     1681 taggtatttt taccggagct aagtcgatgt ttggttccga tgcggagatg ctgcaactag
     1741 cccaacctca ggctgagaat atcatgatgg ggaataattc atctgttcag atgatgcaaa
     1801 ctattgaacc aaaccagttc gatcagattg cagaacaaat tgcctcgcta aatttaccta
     1861 tgctgattat tagctttata gtgtatttta tccttggata tttgctctat agttcgctta
     1921 tggccgctat tgctgcagct gttgattctg aggaagatgt gaaccaattt atgttgccgg
     1981 ttaccatacc tttgatagca gctattgtta tgatggcaaa tgtggtgaaa aatcctgatg
     2041 gggctattgc aatatggggt tctcatattc cttttacttc gcctataatt atgatggtaa
     2101 ggattccctt tggcgtgcca tggtgggaat tgttgatttc aatttctgtg ttggccataa
     2161 ctatgttggg ggccgtttgg gtagctgcaa agatttatcg taccggaatt ctgatgtatg
     2221 ggaaaaaacc tacctggaaa gagttgggga aatggcttaa gtataaaagc tgattaagat
     2281 ataaaaaaac aggctggtaa aaataccagc ctgttttttt atgataacag caatattagt
     2341 tcttgtattg accgttaatt gtaacttttg ttccggcagt aactgattca actttgccat
     2401 tggtgatgtg aagcatgtag taaacatcct gataaactac acctagtaca tagtcgtaag
     2461 atgctgattt agatgcagat attttactcc attttccatc ggcgattgca gtagctgcgt
     2521 caatagcagt tttcagagct tcagcagtag tgatggtagt ccatgcagtc gattcaagtt
     2581 ctacaaattt tgtagctcca ttcgatgcaa catcaatacg ggcaaagaat ccgtcttcaa
     2641 cagaaccaat agttttagtg aaagaa
//
LOCUS       DAOPUW010000248         2663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_40085_length_2663_cov_2.777607, whole genome shotgun sequence.
ACCESSION   DAOPUW010000248 DAOPUW010000000
VERSION     DAOPUW010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40085_length_2663_cov_2.777607"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PKJ43_05110"
     CDS             <1..331
                     /locus_tag="PKJ43_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNZ71979.1"
                     /translation="EEVVNSGTASIARIPGITMCGKTGTAQNPHGPDHSTFIAFAPRE
                     NPKIAIAVYVENGKWGNLYAAPIASLMIEKYLNDSIQPSRQSLETNMLETNLLYPELP
                     NYIQYPK"
     gene            334..1758
                     /gene="rodA"
                     /locus_tag="PKJ43_05115"
     CDS             334..1758
                     /gene="rodA"
                     /locus_tag="PKJ43_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNZ71980.1"
                     /translation="MAQRFEIWKRLDWIIILLYIFLVIAGWFNIYSAVYSDEHRSIFD
                     MEQRYGKQMLWIIFAFVLGLLILVTDSNFFVAFAFPLYIIFLLLLIVVLLFGNEVNGA
                     KAWLTIGDFALQPSEFAKFGTSLALAKVMSSYDFSFKNRNSQILLIALLVIPVLLIVL
                     QHDTGSALVYVAFLIPFYREGMSGLILFFLFFTALLFILSLLLAPWLFVVLLILSAFA
                     VFISLRKKWNELLAPLIVLVSSIALVSIVFLVFGIAYNFYFVLLISSFISGVILLFLS
                     IYKRVPLATLIVSIFILSILFSFTVDFAFENILQPHQQDRINSFLGIESDPLGTGYNV
                     NQSIIAIGSGGLFGKGFLKGTQTKFDFVPEQSTDFIFCTVGEEWGFLGSIIIIVAFIY
                     LIIRIINLAERQRSIFSRVFGYCVASIIFFHFVINIGMTIGIAPVIGIPLPFFSYGGS
                     SLWFFTLLLFTFLRLDASRFEQLR"
     gene            1910..>2663
                     /locus_tag="PKJ43_05120"
     CDS             1910..>2663
                     /locus_tag="PKJ43_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ71981.1"
                     /translation="MARNGAGKTTLLDTIMGKDFPDSGKVTFSNEITVAYLSQVPVLN
                     PDFTVMQEVYHSSSGLVDAVREYEEAILHPQQHNLQSAMEKMDLLNAWDFDVKIKQIL
                     SRLKINNFDQKVSTLSGGQQKRVALANVLINEPDFIILDEPTNHLDLDMIEWLEEYLL
                     KTKSTLFMVTHDRYFLDRVCNEILELDRNTIFRYKGNYSYFLEKREERQEIHQAEVEK
                     ARNLMRTELEWMRRMPQARATKAKYRIDSFYEL"
BASE COUNT          701 a          491 c          541 g          930 t
ORIGIN      
        1 ggaagaggtt gtaaactcag gaacagccag tattgcccgt ataccgggaa ttactatgtg
       61 tggaaaaacg ggaacggcac aaaaccctca tggtcccgac cattcaacat ttattgcttt
      121 tgcgccccgc gaaaatccga agatagccat agctgtttac gtcgaaaatg ggaaatgggg
      181 gaacttgtat gctgctccaa ttgccagctt aatgattgaa aaatatctta acgactctat
      241 acagccttca cgtcaatcgc tcgagactaa catgcttgaa acgaatttgc tgtatcctga
      301 gttgccaaat tatattcaat atccaaaata gctatggcac aacgattcga aatatggaag
      361 aggttagact ggattatcat cctgctttat atctttttgg taattgctgg gtggtttaat
      421 atatactcag ccgtatatag cgatgagcat agaagtattt ttgatatgga acaacgatat
      481 ggcaaacaga tgctatggat tatttttgct tttgtgctcg gccttttaat cctggttacc
      541 gatagcaatt tctttgttgc ttttgctttt cccttatata taatcttttt gcttttgtta
      601 attgtggttt tactttttgg taacgaagta aatggcgcga aagcatggtt aactattggt
      661 gattttgccc ttcagccttc tgaatttgca aaatttggga cttcgttggc tctggcaaag
      721 gttatgagtt catacgattt tagttttaag aaccggaatt cacagatttt gcttatcgct
      781 ctgcttgtga ttcctgtact attgattgtc ctacagcatg atacgggttc tgcattggtt
      841 tatgtagctt ttcttatccc attttatcgg gaaggtatgt cggggcttat cctgttcttc
      901 ttattcttca ctgcgctatt attcatattg tcattgttac ttgctccctg gctttttgtt
      961 gtcttgctta ttctatctgc ttttgcggtg tttatttcct tacgaaaaaa atggaatgag
     1021 ttgttagctc ctttaattgt attggtttct tctattgcac ttgttagtat tgtttttctt
     1081 gtgtttggca tagcttataa cttctatttt gttctattga tttcctcttt catctcagga
     1141 gtaattttgc ttttcctttc tatttataaa agggtacctc tggctactct tatcgtatca
     1201 atcttcatcc tttcgatcct gttctctttc actgtcgatt ttgcctttga aaatattctt
     1261 cagcctcatc aacaagaccg gataaatagt tttttgggca tcgaatcaga tccacttggt
     1321 acaggatata atgttaatca atcaataata gccattggtt ctggcggttt atttggtaaa
     1381 ggttttctaa aaggaactca aacaaaattt gattttgtac ccgagcaaag taccgatttt
     1441 attttttgta cggtaggtga agagtggggt tttttagggt caataataat aatagtagct
     1501 tttatttatt tgattataag gattattaat ttggccgaac gccagcgatc aatctttagc
     1561 cgcgtttttg gctattgcgt tgcatccata atcttttttc actttgtgat aaatattggt
     1621 atgacaatag gtatagcccc ggttattggt attccgttgc ctttctttag ttatggtggt
     1681 tcatctcttt ggtttttcac cttgttgctt tttacttttc ttaggctcga tgccagccgc
     1741 tttgaacagc tgcggtaaat ttgttcgctg ctgtgttttt cctatttttg tgaaaaaata
     1801 attaattata ccatttctgc aagttgaagg gttaacccgt tactggggcg aaaatatcct
     1861 gttcgaatct cttaatttca caatagcaca aggccaaaaa gtagcactca ttgctcgcaa
     1921 tggagccgga aaaacaacgc tgctcgacac tatcatgggt aaagatttcc ccgatagtgg
     1981 aaaggttact ttttcgaatg aaattactgt tgcatacctc agccaagttc ccgtgctaaa
     2041 tccagatttt accgtgatgc aggaagttta tcattcttcg agcggattgg ttgatgcggt
     2101 tcgagaatac gaggaggcaa tcctgcatcc tcagcaacat aacctgcaaa gtgcaatgga
     2161 aaagatggac ttgctgaatg cgtgggattt cgatgtgaaa attaaacaga ttttatcaag
     2221 gctgaaaatc aataatttcg accagaaggt ttcaactctt tctggcggac agcaaaaacg
     2281 ggttgctttg gccaatgttt tgattaatga acctgatttt attattcttg atgaaccgac
     2341 aaaccacctt gatcttgaca tgattgaatg gcttgaagag tatttgctta aaacaaagag
     2401 cacgcttttc atggttactc acgaccgtta cttcctcgac agggtgtgta acgaaatatt
     2461 ggaactcgat cgaaatacaa ttttcagata taaaggaaat tactcttatt ttctcgagaa
     2521 acgggaggaa cggcaagaaa tacatcaggc agaagtggaa aaagcccgca atttaatgcg
     2581 taccgagttg gagtggatgc gacgcatgcc acaggctcgg gccactaaag caaaataccg
     2641 catcgattcg ttttatgagc tta
//
LOCUS       DAOPUW010000249         2651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_40341_length_2651_cov_2.729584, whole genome shotgun sequence.
ACCESSION   DAOPUW010000249 DAOPUW010000000
VERSION     DAOPUW010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40341_length_2651_cov_2.729584"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PKJ43_05125"
     CDS             complement(<1..578)
                     /locus_tag="PKJ43_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71982.1"
                     /translation="MDVIAWIGLSQALFSGIITLSKDGKSISDRLLSAWLFLLAIEFL
                     TFGLESELFPDFILLTNSFLLFNPALCLYTRSLTEPGFKLRWKQLFHLLPYIAFKIIA
                     WEIKEPQSLENFFDINDTLWYRILFACFGIVSWAVYLSMTSIIITRHRSRLKQEFSSI
                     DLFKRVGWIHFVVIIYILYSITVLIWGLFSVIV"
     gene            774..1172
                     /gene="rnpB"
                     /locus_tag="PKJ43_05130"
     ncRNA           774..1172
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKJ43_05130"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1396..1587)
                     /locus_tag="PKJ43_05135"
     CDS             complement(1396..1587)
                     /locus_tag="PKJ43_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71983.1"
                     /translation="MAENTNCENCAMRAKYDQNPKSFGGRFWRWHIGFCPGWKAYMIS
                     LDEGRRKDIASHYNLKKWM"
     gene            1750..>2651
                     /locus_tag="PKJ43_05140"
     CDS             1750..>2651
                     /locus_tag="PKJ43_05140"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNZ71984.1"
                     /translation="MKTKEEQFNSLKLSGIFMLIMNILMLLVISAAFVWAVSTTDLDS
                     FLKISVLVILAVLFIINCIFWGGFMLIEPNEVMVMIFFGKYDGTIKENGFWWVNPFYT
                     KKKLTMRARNLDAEPIKVNDKTGNPIMIGVVVVWRVVDTYKASFNIDSGSMATHVAGA
                     SVPGAVRLNEKMMAYQNFVKIQSDSALRNVAGMYAYDHAGAGSNDELTLRSGGDDINS
                     ILETQLNERLQIAGIEVVEARINYLAYAPEIAAVMLRRQQADAIISAREKIVDGAVGM
                     VKLALDKLSAEQIIELDDDKKAAMV"
BASE COUNT          769 a          462 c          598 g          822 t
ORIGIN      
        1 acaataacac tgaaaagccc ccatatgagc accgtgatgc tatataaaat ataaatgatg
       61 accacgaaat gaatccaccc aacacgttta aaaaggtcga tagatgagaa ttcttgtttc
      121 aatcggcttc ggtgacgggt aattataatg cttgtcatgc taagataaac cgcccaagat
      181 acaattccga aacatgcaaa caaaattctg taccatagtg tgtcattgat atcaaaaaag
      241 ttctcgagcg attgaggttc tttaatttcc caggcaataa ttttgaaagc gatatacggc
      301 aaaaggtgaa aaagctgttt ccacctgagt ttaaagccgg gctcagtgag cgaacgcgta
      361 tataggcaaa gtgccgggtt gaataataaa aacgaattgg tcaggagaat aaaatcggga
      421 aagagttccg attcaagtcc gaatgtaaga aattcaatgg caagcaagaa aagccaggcc
      481 gaaagcagtc ggtccgatat gcttttccca tctttcgata gggtaataat ccccgagaaa
      541 agtgcttgag ataaaccaat ccaggcaatt acatccataa gttatttgtg tttttctgta
      601 aatttagcta aagtgagtct aatttgcttg ttttgtgtgt attatatttt gttgttaatt
      661 gggcaagtgt gtcatgtatt cttgcatttc agattttggc aaagacggct tttcttggtt
      721 tattttgtta cttttgccgc aaagcagatc gttctatcgt tcggaacaat gatttggaag
      781 ggcgactttt caagttgttt tccgggagga aagtccgggc aacacagggc attccgctct
      841 ttccccggcc tgagtttatc aggatcgggg aggagatgtt cgtgagggca tggtaacgga
      901 gaagaaaata accgtctcgg tctcttcgga gggcgtgata agggtgagaa ggtggggtaa
      961 gagcccacca ggttgagtgg cgacattcaa tgctgtccgt cctggaagtt gcaaaaccaa
     1021 atatatcctg aagcttcggc cagcagctgc ccgttgcagt ttcttcactt gtggggaagt
     1081 tcaggagggg taggtcgctt agataaatga taggagcacc gttttcgaaa gatctcggtg
     1141 tacagaaccc ggcttacaga tctgcttttt ctttttttaa tacagaaaca tcttcttgta
     1201 tttcagttgt gtgttcttga gtgaaagaaa tacatcaact ataaatgagg gaaaagtata
     1261 actcaacccc taaagggtat tctacttttc acctcatttg gttaaagtgt gcagtgaaaa
     1321 tgggatgaag tatctgcaaa gcacttgata atagtgtgtt aagtgtggtg tactgaggct
     1381 ttttttagta aactgttaca tccatttctt aaggttgtaa tgggatgcaa tgtcttttct
     1441 cctgccttca tcgagcgaga tcatgtacgc tttccacccg ggacaaaatc caatatgcca
     1501 tctccagaat ctaccaccaa acgatttggg gttctggtcg tattttgcgc gcatcgcgca
     1561 gttttcgcaa tttgtgttct ctgccataat attttgatgt tgattttact attagtatgt
     1621 gcccatctgc aatcattaca atttttcata ttttttttca tcgatttaaa aagtgttgaa
     1681 aatgtttgga gtataagaat atttctctac atttgtgata tcaaaataat atcaaaataa
     1741 attaactcta tgaaaacaaa agaagaacaa tttaactcct tgaaattatc gggaattttt
     1801 atgttgatca tgaatatact tatgcttttg gttatttcag cagcatttgt gtgggcagtg
     1861 tcgacaaccg accttgattc gtttttgaaa ataagtgtgt tggtaatatt ggcagtactt
     1921 tttattatta attgtatttt ttggggaggt ttcatgctta tcgaacccaa tgaggtgatg
     1981 gtcatgattt tctttggaaa atacgatggt acgataaaag aaaatggctt ttggtgggtt
     2041 aatccctttt ataccaagaa gaaacttaca atgagggctc gaaacctcga tgctgaacca
     2101 atcaaagtga acgacaagac tggtaaccca attatgattg gggttgtggt tgtttggcgt
     2161 gttgtcgata cttacaaagc ttcttttaat atcgacagcg gttctatggc tacacatgtt
     2221 gcaggcgctt cggttcccgg tgcagttagg cttaatgaaa aaatgatggc ttaccaaaac
     2281 tttgttaaaa ttcagagcga ttctgctttg cgtaacgtgg ccggaatgta tgcatatgac
     2341 catgcagggg ctgggagtaa cgatgaactt acccttcgtt caggtggcga tgatataaat
     2401 tcaattttgg aaacccaatt gaacgaacgt cttcaaattg ctggcattga agtggttgaa
     2461 gcacgtatta attatttggc gtatgctccc gaaattgctg cagttatgct tcgtcgccaa
     2521 caagctgatg ctattatttc tgctcgtgaa aaaattgttg acggggctgt agggatggtg
     2581 aaacttgctc ttgataaact ttcagcagaa caaataattg aactcgacga tgacaaaaaa
     2641 gctgcaatgg t
//
LOCUS       DAOPUW010000250         2650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_40353_length_2650_cov_3.694027, whole genome shotgun sequence.
ACCESSION   DAOPUW010000250 DAOPUW010000000
VERSION     DAOPUW010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40353_length_2650_cov_3.694027"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="PKJ43_05145"
     CDS             <1..1069
                     /locus_tag="PKJ43_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71985.1"
                     /translation="DNAPYDTNYFLAKNQHALNNFKIATSFYNEFSKLATKIERKELQ
                     IDKLTGLCKKSINPFTVSEDPEIFLNRLNEKRKANKEKVREEEIVEIPAELIDTTINF
                     IISSEIYYSRIGQFKTNQGKKNFIGGWTNAGLLKILMQEVDSLRTEYSKSYSSENKHY
                     LSTKVLDLEQKIMRTKNLSDEKLMLANKLEAEWWKKAQEDEKWKLAAQNDSIRKADEE
                     KIMKQQLPVILPQMEEPADTTAQLTDSTTIQETEPITEVPESTANQVVFKIQIGKYNT
                     QLPESTKKLFKKISVLRKIDQYTDENNYIVYTIGELTNIKDAVKLQNQIRQESVKDAF
                     VIAIKDGKRIPLNEALKLTGQ"
     gene            1066..1344
                     /locus_tag="PKJ43_05150"
     CDS             1066..1344
                     /locus_tag="PKJ43_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNZ71986.1"
                     /translation="MTKEKYKEIPRFEDSALNDQEHELVLLNDDVHTFDYVIDALIKI
                     CDHTPQQATQCTLLIHYKGSCDVKKGDIKTLRPLRKALIEKELNAKIV"
     gene            1384..1854
                     /locus_tag="PKJ43_05155"
     CDS             1384..1854
                     /locus_tag="PKJ43_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNZ71987.1"
                     /translation="MEILIIIMLIILGIVLLFVEFMLIPGVSIAGVGSLLSFAGAVLF
                     AWKTMGSAGGIITLVVELIIVPTLLYLIFKGKAMKPMMLETDIDGKMKNIEEGQIKPE
                     DIGETIGRLAPAGKAKINGIALEVRTNGQFVDPHTKIKVLKIEGNSVYVEPIKE"
     gene            1858..>2650
                     /gene="floA"
                     /locus_tag="PKJ43_05160"
     CDS             1858..>2650
                     /gene="floA"
                     /locus_tag="PKJ43_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422830.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HNZ71988.1"
                     /translation="MTQLPIFLLIVGAVILLFIFLYFIPIGIWFSALVSGVRISLMQL
                     FLMRFRKVPPGVITRAMIEAHKAGLGFVKRDELEAHFLAGGHVENVVHALVSADKANI
                     ELTFKMATAIDLAGRDVLEAVRMSVNPKVINTPPVSAVAKDGIQLICKARVTVRANIK
                     QLVGGAGEETVLARVGEGIVSSIGSSQSHKTVLENPDYISKVVLDKGLDAGTAFEILS
                     IDIADIDIGKNIGAYLQMDQAEADKNIAQAKAEERRAMAVAVEQEM"
BASE COUNT          909 a          536 c          541 g          664 t
ORIGIN      
        1 cgacaatgcc ccatacgata caaactattt tttggcaaaa aaccaacatg ccctcaataa
       61 tttcaaaatt gcgacttcgt tttataacga attctcaaaa cttgccacaa agattgaaag
      121 aaaagaactt caaattgaca aacttactgg cctttgcaaa aagagcatca atccatttac
      181 cgtttctgag gatcctgaaa tctttcttaa ccgcttgaat gaaaaaagaa aagcaaacaa
      241 agaaaaggtc cgcgaagaag aaattgttga gattcccgcg gagcttatcg acaccacgat
      301 taatttcatt atcagctccg aaatatacta ttctcgaata gggcaattta aaacaaacca
      361 agggaaaaag aactttattg gcgggtggac aaatgcagga ttacttaaaa tattgatgca
      421 agaagtcgat agtctgcgta ccgagtattc aaaatcatat tcaagtgaaa ataagcatta
      481 tttgtctaca aaagtgctcg atctggagca aaaaatcatg cgcaccaaga acctgtcaga
      541 cgagaaacta atgcttgcca ataagttgga agcagaatgg tggaaaaaag cccaagaaga
      601 tgaaaaatgg aaactggcag cgcaaaatga ctcgatacga aaagccgacg aagaaaaaat
      661 aatgaaacag caattgcctg tgatacttcc ccaaatggaa gaacctgccg acacaacagc
      721 gcaacttacc gacagcacta caattcaaga aactgaacca ataacagaag ttccggaatc
      781 aacagctaat caggttgttt ttaagataca aattgggaaa tacaatacac agcttcctga
      841 gtcgacaaaa aagttgttca agaaaatatc ggttctccga aaaatagatc aatataccga
      901 cgaaaataat tatattgtat acaccatagg cgaactcacc aacataaaag atgccgtaaa
      961 acttcagaat caaattcgtc aggaaagtgt taaagatgcg tttgttattg caataaaaga
     1021 cggaaaaaga atacctttga acgaagcact aaaattgact ggccaatgac caaagaaaaa
     1081 tataaagaaa ttcctcggtt tgaagattct gcgctaaatg atcaggaaca cgagctggta
     1141 ctactaaacg atgatgttca cacctttgat tatgtgattg atgcattgat taaaatatgc
     1201 gaccacacgc cacaacaagc tacacagtgc actttactta ttcattataa aggaagctgc
     1261 gatgtaaaaa aaggggacat caaaacattg cgcccattac gcaaagccct tattgaaaag
     1321 gaactaaatg caaagattgt ttagccggat tatttttcat aacttaaacc ctttaactac
     1381 actatggaaa tactaattat catcatgctc atcatcctcg ggattgtact cctttttgtt
     1441 gagtttatgc ttatccccgg agtttcgatt gcgggagtag gcagtttact gtcatttgcc
     1501 ggagctgttc tttttgcatg gaaaacaatg ggaagtgccg ggggaattat tacactggta
     1561 gttgaattga ttattgttcc aacgctttta tacctcatat tcaaagggaa agcaatgaaa
     1621 cccatgatgc tcgaaaccga tatcgacggg aaaatgaaaa atattgaaga aggtcaaata
     1681 aagccagaag atatcggcga aaccattggc cgcttggccc ctgcaggaaa agcaaaaatc
     1741 aatggaatag cccttgaagt tagaacaaac ggacaatttg tcgatcctca cacaaaaata
     1801 aaagtcctta aaatagaggg caactcagta tatgtagaac caataaaaga ataaaagatg
     1861 actcaattac ccattttttt actcatcgta ggagcagtaa tactcctatt cattttcctc
     1921 tactttatcc ctattgggat ttggttttcg gcacttgtgt cgggcgtgcg tatctcgctg
     1981 atgcaattgt tccttatgcg ctttcggaaa gttcccccgg gagttatcac ccgcgccatg
     2041 attgaggcac acaaagcagg tcttggtttt gtgaagcgcg acgaacttga agcccacttt
     2101 ctggctggtg gccacgttga aaatgtggta catgctctgg tatcggctga taaagcgaac
     2161 attgaactta cctttaaaat ggctacggcc attgaccttg ccggccgcga tgtgctcgaa
     2221 gctgtacgta tgtcggtaaa tccaaaggta attaacactc ctccggtttc ggcagttgca
     2281 aaagacggaa tccagcttat ttgtaaagca agggtaaccg ttcgtgccaa cattaaacaa
     2341 ttggtaggtg gtgccggcga agaaactgta cttgcccgtg taggcgaagg gatcgtttcg
     2401 tcgatcggat catcgcaatc gcataaaacc gtgctcgaga accccgatta catttcaaaa
     2461 gttgtacttg acaaaggact cgatgccggt actgcctttg aaatcctttc cattgatatt
     2521 gccgacattg acattggcaa gaacattgga gcatacctgc aaatggacca ggccgaagcc
     2581 gataaaaata ttgctcaggc gaaggccgaa gaacgacgtg caatggctgt tgctgttgaa
     2641 caggaaatga
//
LOCUS       DAOPUW010000251         2645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_40468_length_2645_cov_3.308494, whole genome shotgun sequence.
ACCESSION   DAOPUW010000251 DAOPUW010000000
VERSION     DAOPUW010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40468_length_2645_cov_3.308494"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PKJ43_05165"
     CDS             complement(<1..582)
                     /locus_tag="PKJ43_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HNZ71989.1"
                     /translation="MENFKHHIKKAIVLGSGALKIGEAGEFDYSGSQALKALKEEGVE
                     TILINPNIATIQTSDEIADKFYFLPVTPYFVEEVIKKEKPQGILLAFGGQTALNCGVA
                     LYQSGVLEKYNVEVVGTPVQSIIDTEDRDIFSNILKSIDVKTPKSIACANMDEAKAAV
                     KEVGFPIIIRAAYTLGGLGSGFCDNMEELETRAA"
     gene            complement(606..1682)
                     /gene="carA"
                     /locus_tag="PKJ43_05170"
     CDS             complement(606..1682)
                     /gene="carA"
                     /locus_tag="PKJ43_05170"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNZ71990.1"
                     /translation="MKRVKLILEDGTVFTGKSFGSERSVAGEVVFYTAMVGYPESLTD
                     PSYTGQILVSTYPMIGNYGVPADSKNDGINIHYESDKIHASGLIISDYSFEYSHWNAV
                     KSLGDWLKENNVPGIFDIDTRALTKILREKGSMLGKIEFENDPIEFYDPNLENLVAIA
                     SIKEPITYGNGKYNVVLVDCGTKNNIIRCLLNRGATVKRVPWDYDFTTEEYDGLFISN
                     GPGDPAMCDITVKNIAKALSENKPVMGICLGNQLLALAAGAKTYKLKYGHRSHNQPVL
                     LNNTNRCFITSQNHGYAIDNESLPSDWEILFQNVNDKTNEGIRHKTKPFFSTQFHPEA
                     ASGPVDTEFLFDEFIGNISKYKGE"
     gene            1860..2399
                     /locus_tag="PKJ43_05175"
     CDS             1860..2399
                     /locus_tag="PKJ43_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HNZ71991.1"
                     /translation="MLNIFASKWNIHAFFMKAKLQRQVAIRNIISQNKVTSQEELLML
                     LKSKGFESTQATLSRDIKMLKIAKTPDPHGGYVYVLPAEILAAKGSGERRAPGRGFVG
                     EGFVSIDFSGNLAVIKTKPAYASSIALLIDEAASYVILGTIAGDDTIFLVLREGVEKG
                     DVIDFLEKIMPSAKNKLIL"
     gene            2408..>2645
                     /locus_tag="PKJ43_05180"
     CDS             2408..>2645
                     /locus_tag="PKJ43_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ71992.1"
                     /translation="MDEREKIVVLVADESHLKYVDEINDTIQRASEERGTGIARRTYD
                     YVATKMKEGKAIIALDGEKFAGFCYIESWGHHKFV"
BASE COUNT          696 a          517 c          599 g          833 t
ORIGIN      
        1 tgcagcccgg gtttcaagtt cttccatgtt gtcgcaaaag cccgaaccga ggcctccaag
       61 cgtgtatgct gcacggatga ttatagggaa tcccacttct ttcaccgcgg ctttagcttc
      121 gtccatgttc gcgcaagcta tgcttttggg cgtttttaca tcgatgcttt tcagtatgtt
      181 ggagaaaata tcacggtctt cggtgtcaat gatcgattgt accggggtac caaccacttc
      241 tacattgtat ttctcaagta ctcctgactg ataaagtgca accccgcagt tgagtgctgt
      301 ttgacctcca aatgccaaaa gtatgccttg tggtttttcc ttttttataa cttcttcaac
      361 aaaatatggg gtaaccggaa gaaagtaaaa cttgtcggca atttcatcgg aggtttgtat
      421 tgttgcgatg ttcgggttga taagaattgt ttcaacccct tcttctttca gtgcttttag
      481 tgcctgggag cctgagtaat cgaactctcc ggcctcgcca attttcaatg cacccgaacc
      541 aagaacaatt gcttttttaa tatgatgttt gaaattttcc attttgtgtc tctttaaggt
      601 caatcttact ctcctttata ttttgaaata ttcccgatga attcgtcgaa taagaattcg
      661 gtgtcgacag gcccgcttgc ggcttcgggg tgaaattgcg tggaaaagaa gggtttcgtc
      721 ttgtgacgta tgccttcgtt ggtcttatcg ttcacatttt ggaataatat ttcccagtcg
      781 gagggcaatg actcattgtc gatggcatac ccatgatttt gcgatgtgat gaaacagcgg
      841 tttgtattgt tgagcagtac tggctggttg tggctgcggt gcccgtattt gagcttatag
      901 gttttggctc cggcagcaag tgcaagaagt tggttgccaa ggcaaattcc cataacgggt
      961 ttgttctctg aaagtgcttt ggcgatgttc ttaacggtaa tgtcgcacat agccgggtcg
     1021 ccaggtccgt ttgaaataaa gaggccgtcg tactcttcag ttgtaaagtc gtaatcccag
     1081 ggaacccttt tcactgtggc tccacggttt agcaagcatc gtatgatatt gttttttgtg
     1141 ccgcaatcga ccagaacaac gttgtatttt ccatttccgt aggtgatggg ttctttaatg
     1201 ctggcaatgg caacaaggtt ttcgaggttg gggtcgtaga attcgatcgg gtcattttca
     1261 aattcaatct tgccaagcat cgatcctttt tcacgcaata tcttggtcaa tgcgcgggtg
     1321 tctatatcga aaattcccgg cacgttgttt tctttgagcc aatcacccag gctttttacc
     1381 gcattccaat ggctgtattc aaacgaataa tccgaaatga tcagtcctga tgcgtggatc
     1441 ttatccgatt cgtaatgaat gttgatccca tcgttttttg aatccgccgg gaccccgtaa
     1501 tttccgatca ttggatatgt tgatactaga atctgccctg tgtatgaagg gtctgtaagg
     1561 ctttcagggt agccaaccat agcggtatag aaaactactt cgccggcaac cgaccgctcg
     1621 ctgccaaacg actttccggt aaaaaccgtt ccgtcttcaa ggattaactt tactcttttc
     1681 aaggttgatg gtattactat aattataaaa aaaatgcgct tatctgctta ggcagttaaa
     1741 cgcatcgcga atgtatcttt atatcccgta taagaatttt cactttatct cggttatgtt
     1801 attttaaatt cgatggcaaa aataataaag tttcttgaaa aaatgttgga tgtttcacaa
     1861 tgttgaatat ttttgcatcg aaatggaata ttcatgcatt ttttatgaaa gcgaaattac
     1921 aacggcaggt agcaatccgt aacattatta gtcaaaataa ggtcacaagc caagaggaac
     1981 ttctaatgtt attaaaaagt aaaggatttg aatcgactca agcaacgtta tcgcgcgaca
     2041 ttaaaatgct gaaaatcgcc aaaacacccg atcctcatgg tggctatgtt tatgttcttc
     2101 ctgcagaaat tcttgccgca aaaggttctg gagaaagacg tgcacctggg cgtggttttg
     2161 ttggcgaagg tttcgtttct attgattttt caggaaacct tgccgtgata aagacaaagc
     2221 ctgcctatgc cagcagcatt gctttgctta tcgacgaagc tgcttcgtac gtaatattgg
     2281 gaactatagc tggcgatgat accatttttc tggtattgcg cgaaggggta gaaaaagggg
     2341 atgtaattga ttttttggaa aagattatgc caagtgcaaa gaataaattg atattgtaat
     2401 atttaagatg gacgaaagag aaaaaattgt ggtgttggtt gccgacgagt ctcacttgaa
     2461 atatgtcgac gaaataaatg atactatcca gcgtgcttct gaagaaaggg gaacaggaat
     2521 tgcacgccgt acatacgatt atgtagctac taaaatgaaa gaaggcaagg caataattgc
     2581 cttagatggt gaaaaatttg ccggtttttg ctatatcgaa tcttggggac atcataagtt
     2641 tgttg
//
LOCUS       DAOPUW010000252         2639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_40592_length_2639_cov_4.165248, whole genome shotgun sequence.
ACCESSION   DAOPUW010000252 DAOPUW010000000
VERSION     DAOPUW010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40592_length_2639_cov_4.165248"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..1918
                     /gene="lysS"
                     /locus_tag="PKJ43_05185"
     CDS             188..1918
                     /gene="lysS"
                     /locus_tag="PKJ43_05185"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNZ71993.1"
                     /translation="MSIRELSEQEIIRRESLNKLRELGINPYPADEYTITNSCAELKE
                     KYNPEENNFQEVSIAGRLMGQRIMGKASFAEIQDESGRIQLYINRDELCPGEDKTLYN
                     DVFKKLFDIGDIIGVKGFVFITQMGETSVHVKELVLLSKSLRPLPIVKEKDGQTFDAF
                     TDPETRYRQRYVDLIVNPLVRETFKNRTKVINTMRQMFNERGYLEVETPILQPIPGGA
                     AARPFITHHNALDIPLYMRIANELYLKRLIVGGFEGVYEFAKDFRNEGMDRTHNPEFT
                     VMEIYIAYKDYHWMMDFTEEMIERVALALHGTTTVQVGENSIDFKRPFKRISILDSIK
                     EHTGFDVSGMDEEQLREVCKKLDIEVDNTMGKGKLIDEIFGEKCESNYIQPTFITDYP
                     KEMSPLCKKHRDNPDLTERFELMVNGKELCNAYSELNDPIDQLERFEEQLRLSEKGDD
                     EAMFIDMDFVRALEYGMPPTSGMGIGIDRLVMFMTNQPSIQDVLFFPQMKPEKIVKTD
                     SKDDFVAAGIPEEWVEPLFKLGYKTLAKLKSVEKPAKLHQEICGFNKKNKLGLANPSQ
                     DEVGQWVENA"
     gene            complement(2010..2552)
                     /locus_tag="PKJ43_05190"
     CDS             complement(2010..2552)
                     /locus_tag="PKJ43_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71994.1"
                     /translation="MNLPCSDFSPHSAMALLRAYLNAVYGVGEWILMYNEEQVFLNHE
                     LIEEKEIKLDDMQQKTALFLNQFEGIKAAVPASLIELSNFDNRRFQIIENSYCIQRSG
                     DVMLILEEGWYPVPRYNEVEYSAENRFPVVFYGANIKPGILDTPIEAIDILPTLCNYL
                     NILPPDGAKGKVVKSVFWYN"
BASE COUNT          802 a          516 c          600 g          721 t
ORIGIN      
        1 aaacagggca accatgatag tacacgattg ccctgacaag tattctaatt gaaaaatttt
       61 aaaaatgact ggcagaataa agtttacatc aagctttttt gcatgagggc aagcgaaaaa
      121 agattttccg acttggtttt tcctatttgc ttttatcttt gcaaaaaatt tataaaaggg
      181 gctaagtatg agtatccgag aattaagtga acaagaaatt atacgtcgcg aatcgttgaa
      241 taaattacgg gaactgggca ttaatcccta tcctgccgac gagtatacta taaccaattc
      301 gtgtgctgaa ctgaaagaaa aatacaatcc tgaagagaat aattttcagg aggtttcgat
      361 agctggacgc cttatgggac agcgcattat gggaaaggct tcgtttgccg aaatccagga
      421 tgaatcggga aggattcagc tctacatcaa tcgcgatgag ctatgcccgg gagaagataa
      481 aactctttac aacgacgttt tcaaaaagct tttcgatata ggtgatatca ttggggtaaa
      541 aggttttgtt tttataaccc agatgggtga aaccagtgtt catgtaaaag aattggtgct
      601 tttaagcaaa tccttgcgtc cgttgcccat tgtaaaagaa aaagatggac aaacattcga
      661 tgcttttacc gacccggaga cccgttaccg ccagcgctat gtcgacttga ttgtgaatcc
      721 gctcgtacgc gaaaccttca agaaccggac aaaggtgatt aataccatgc gccagatgtt
      781 caacgagcgg ggttatctcg aagtcgaaac cccaattttg cagcccatcc caggaggtgc
      841 agctgcgcgg ccattcataa cccaccacaa cgcgctcgat attccccttt acatgagaat
      901 tgcgaacgag ttgtatctta aacgtctcat tgtaggaggg ttcgaggggg tctatgaatt
      961 tgcaaaagat ttcaggaacg aagggatgga ccgtacccat aaccccgaat ttacggttat
     1021 ggaaatttat attgcctaca aagattacca ttggatgatg gactttaccg aagagatgat
     1081 tgagcgggta gccctagccc ttcatggaac aacaacggtt caggttggcg aaaatagtat
     1141 tgattttaaa cgtcctttca aacgcatttc aattctcgac tcgataaaag aacataccgg
     1201 gttcgatgtt tccgggatgg acgaggaaca gttgcgtgaa gtttgtaaaa agcttgatat
     1261 tgaggtggat aacacaatgg gaaaaggaaa gcttattgat gaaatctttg gtgaaaaatg
     1321 tgagtcaaat tatatacaac ccacattcat aaccgattac ccaaaagaaa tgtcgccatt
     1381 gtgcaagaaa caccgcgata atcccgacct caccgagcgc ttcgagctaa tggttaatgg
     1441 gaaagaactt tgtaatgcat attctgagct taacgatccg attgatcagc ttgaaaggtt
     1501 tgaagaacag ttgcgacttt ctgaaaaagg cgacgacgaa gcaatgttta tcgacatgga
     1561 ttttgtccgt gcccttgaat acggaatgcc cccaacttcg ggaatgggaa ttggaatcga
     1621 ccgtttggta atgtttatga cgaatcagcc ttcgatacag gatgtgctgt ttttcccaca
     1681 aatgaaacct gaaaaaattg ttaaaaccga ttcgaaagat gattttgtgg cagccggtat
     1741 cccagaagag tgggtagaac cacttttcaa gttaggatac aagacccttg caaagttgaa
     1801 gtcggttgaa aaaccagcta aacttcatca agagatttgt gggttcaata agaaaaacaa
     1861 acttggcttg gcaaacccat ctcaagatga ggttggacaa tgggttgaaa atgcttgatg
     1921 taatcatgaa taaaatagca aaggggctga aagccccttt tttttttcca gtattgagtt
     1981 gattgtacga acctgcatgg tttgaaattt taattatacc agaacacact ttttactacc
     2041 ttgcctttgg caccgtcggg cggaagtatg ttcaaataat tgcaaagtgt tggcaaaatg
     2101 tcgattgctt caataggagt gtcgaggatt ccaggtttaa tgtttgctcc ataaaataca
     2161 actgggaagc gattttcagc cgaatattcc acttcgttgt aacggggcac cgggtaccat
     2221 ccttcctcaa gaatgagcat tacatcgccc gagcgttgga tgcagtagct gttttcaatt
     2281 atctgaaaac ggcggttatc gaagtttgac aattcaatga gcgaagcagg aactgcggcc
     2341 ttaatgcctt caaactggtt gaggaacagg gctgttttct gttgcatgtc atcgagctta
     2401 atttcctttt cttcaatgag ctcgtggttg aggaacactt gttcttcgtt atacatcaaa
     2461 atccattcgc caactccata tacagcattt aggtatgccc gaagcagggc catggcactg
     2521 tgtggggaaa aatcgctgca tggaaggttc atttcctctt taagaacatc gggtggataa
     2581 cctccggtag aagccagtca ggaataccat ataattctct ttgccaattt tccggtcga
//
LOCUS       DAOPUW010000253         2637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_40637_length_2637_cov_2.783888, whole genome shotgun sequence.
ACCESSION   DAOPUW010000253 DAOPUW010000000
VERSION     DAOPUW010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40637_length_2637_cov_2.783888"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..2567
                     /locus_tag="PKJ43_05195"
     CDS             192..2567
                     /locus_tag="PKJ43_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNZ71995.1"
                     /translation="MVAVKKSRSNVIRIIWVLVGTIIVSTILFFTLISNGAFGFMPTF
                     KELENPKSVVATEIITTDNKTLGRFYKDENRSLAEFKDLSPNLVKALIATEDERFYGH
                     SGIDLKSFGRAVKGILTGDSSSGGGSTISQQLAKLLFPRESNSGLKLIIRKFREWVIA
                     VKLERSYTKEEIITMYLNKFEFINGAFGVQVAARTYFNKTPDALTLEQAALLVGMLKN
                     PFLYNPTRFEDRALQRRNVVLGQMYKNKYISKQEFEKSKALPLNIQFQREELKKGIAP
                     YFRKYLERTLIASQPKRSDYGKSALSEQKYHEDSIAWVTDPLYGWCNKNRKPDGEAYD
                     LYKDGLKIYTTIDYRMQKYAEESVEAHLKSLQPKLNNSIREYKNAPFSDDLTDEQVEK
                     MIMNEITRSERYKNMKAEGKNKEEILREFNKRQLLPVYTWSGYKDTLISPLDSIKYYL
                     KHLSSSFMAMDPRTGEVKAWVGGSAYGFTEIDMVRSSTFKRQVGSTCKPFLYTLAMQN
                     GISPCEKVPNVPVTFTEADGLDAPWTANNSSKTDYDGKMVSLRWGLANSVNQVSAYLM
                     KQFNPEAMKKVMQQMGVYSNITAVPSMFLGTAEITLYEMVAAYSVFANKGVYTTPMIV
                     TRIEDKNGNLLATFQPKKRDALDEQTAYLMTYLLQGVVREGTGVRLATTYEVFKDYGG
                     FKAPFAGKTGTTQNQSDGWFIGFTPELVAGVWTGANYRSIHFKGISLGQGSNTALPVF
                     GRFFKKVFADPTLGLSQNFEFEKPAYMTVDLNCDETDVDSETSKPEYDDFF"
BASE COUNT          864 a          523 c          538 g          712 t
ORIGIN      
        1 tgaaagctat gttcaaaatt tatttacaac tcatttcaat ttatttttta ttttgcctgt
       61 ttttatacaa tataattata tgtgtgaacg gttaaacaag ggaaaattga atttagtaaa
      121 ttttaaacct tatccttttt tataaatatc gtacctattt tatttcttta tctttgtaaa
      181 aattttctca aatggtagct gtaaaaaaat cacggagcaa cgttatccgt ataatatggg
      241 ttttggttgg aacgattatc gtatcaacaa tactgttttt cacattaatt tcaaacgggg
      301 cctttggctt catgccgaca ttcaaagagt tagagaaccc gaaaagcgtg gttgccaccg
      361 aaataatcac caccgacaat aaaacactcg gaaggtttta caaagatgaa aaccggtcgt
      421 tagccgaatt taaagacctg agtcccaatc tggttaaagc attgatagcc accgaagatg
      481 agcgtttcta cggtcactca ggcatcgacc taaagagttt tggccgtgca gtaaaaggaa
      541 tacttaccgg cgatagcagt agtggtggag gtagtaccat ttcacaacag cttgcaaaat
      601 tgcttttccc tcgcgaaagt aattctggat taaaacttat aatcagaaag tttagggaat
      661 gggtaattgc agttaagctt gaaagaagct acaccaaaga agaaattatt accatgtacc
      721 tcaataagtt tgaatttata aacggagcct ttggggttca ggtagccgcc agaacttatt
      781 tcaataaaac acctgatgct ctaacgctag agcaagctgc cctattggtt ggaatgctta
      841 aaaacccctt tctgtacaac cctacccggt ttgaagatcg tgcattacag cgccgtaacg
      901 tagtcctggg ccaaatgtat aagaacaaat acatttcaaa acaagaattc gagaagtcta
      961 aggcgttgcc attaaacatc cagtttcagc gagaagaact taagaaaggg attgccccat
     1021 atttcagaaa atatcttgaa aggaccttga tcgcatcaca gcccaaaaga tcagactatg
     1081 ggaagagtgc cctttcggaa caaaaatatc acgaagattc aattgcctgg gttactgatc
     1141 ccttgtatgg ttggtgtaac aaaaaccgca aacccgatgg tgaagcatat gatttgtaca
     1201 aagatgggct aaaaatatat acaacaatcg attacagaat gcagaaatat gctgaagaat
     1261 cggttgaggc acatcttaaa agccttcagc ctaaattgaa taatagcatc agagaataca
     1321 aaaatgctcc tttctcggac gatcttaccg acgaacaagt cgagaaaatg attatgaacg
     1381 aaataacccg aagtgaacgg tataaaaaca tgaaagccga gggaaaaaac aaagaagaga
     1441 tattgcggga gtttaacaaa cgacaattat tgccggtgta cacatggagt gggtataaag
     1501 acaccttgat ctcacctctc gattcaatta aatattacct aaagcatcta agttcatctt
     1561 ttatggccat ggatccacgc accggggaag ttaaagcctg ggtaggtggc tcggcgtatg
     1621 gtttcaccga aatagacatg gttaggtctt caacgttcaa acggcaggtg ggttcaacat
     1681 gcaaaccatt cctttacact ttagccatgc aaaacggaat ctctccatgc gaaaaagtac
     1741 ctaatgtgcc cgtaacattt actgaagccg acggattaga cgctccatgg actgcaaaca
     1801 attcatcgaa aaccgattac gacgggaaaa tggttagctt gcgttgggga ctggctaact
     1861 cggtaaacca agtttcagcc tacctgatga agcagtttaa ccctgaagca atgaaaaagg
     1921 taatgcaaca aatgggagtg tacagcaata ttactgccgt tccttctatg ttccttggta
     1981 ctgccgaaat cactctctat gaaatggttg cagcatattc agtatttgcg aacaagggag
     2041 tttacactac acctatgatt gttacccgta ttgaggacaa aaatggtaac cttcttgcaa
     2101 cctttcagcc taaaaagcgc gatgctcttg acgaacaaac agcttacctt atgacttacc
     2161 tgttgcaagg tgttgtacgc gaagggactg gcgtacgtct tgccacaacc tatgaagttt
     2221 ttaaagatta cggagggttt aaagcaccat ttgccgggaa aacaggaacc actcaaaacc
     2281 aaagcgatgg atggttcatt gggtttacac ccgaattggt tgccggcgtt tggacaggtg
     2341 caaattaccg aagcattcac ttcaaaggta tttcactcgg gcaaggttcg aatactgcac
     2401 tccctgtttt tggccgtttc tttaagaagg tttttgcaga cccaactcta gggctttcgc
     2461 aaaactttga atttgagaaa ccagcctaca tgactgtcga tttaaattgc gacgaaactg
     2521 atgtagattc agaaacatcg aaacccgaat atgacgattt cttctaaaaa agacaacaga
     2581 acaatcggaa agctctttag catttgggtt ttcaaataaa taacttgaag cgttcaa
//
LOCUS       DAOPUW010000254         2628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_40826_length_2628_cov_2.902837, whole genome shotgun sequence.
ACCESSION   DAOPUW010000254 DAOPUW010000000
VERSION     DAOPUW010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40826_length_2628_cov_2.902837"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PKJ43_05200"
     CDS             complement(<1..214)
                     /locus_tag="PKJ43_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ71996.1"
                     /translation="MIRIFKDTFGCILGAFLFSGISVSVSGQSEVLVHWRFEQIQHLN
                     GDSTSVSIVGQGVQAEQICYSTAKNIN"
     gene            complement(238..1608)
                     /locus_tag="PKJ43_05205"
     CDS             complement(238..1608)
                     /locus_tag="PKJ43_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HNZ71997.1"
                     /translation="MKKVSYILLIVVAFAGSLSAQNPIVQTYFTADPAPMVYDGTVYL
                     YTSHDEDSTVNNFFTMYDWRCYSSKDMVNWTDHGAVASLKNFTWMNKDNGAWAPQCIE
                     RNGKFYLYVPIHGEGIAVLVSDSPTGPFTDPIGKRLIDSDHIWQDIDPTVAIDEKGQA
                     YLYWGNPSLWYVKLNEDMISYDKSVGANGIVSIEMTPQSFGSKAGRDGRPGTTYTEGP
                     WFFKRNNLYYVMYASEGIPEYISYSTSNSPEGPWTYKGQIMKRADHLAFTNHAGIIDF
                     KGNSYIFYHDQDLSKGQGFKRSVSVEQFTYNADGSIPLITPTKEGVTKSVANLDPYKK
                     VEAETIAFSEGLKTASDSKTGVYVTKIENGDYLKVRSVDFAKGAKTFEASVASASAGG
                     KIEIRLGDRNGELLGTIDVKNTGGLQKWATVKGKVKKATGVQDICLVFKGGEGELFNF
                     DWWAFN"
     gene            complement(1630..>2628)
                     /locus_tag="PKJ43_05210"
     CDS             complement(1630..>2628)
                     /locus_tag="PKJ43_05210"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HNZ71998.1"
                     /translation="GVAVGEKPEGLFKDALGKPLLTGYGYIDPTVFIDDYGQAYLYWG
                     NPNLWHVKLNEDMISFDETYGIKKEDLKDENFGYRAKKIDNRTAAYEEGPWLYKRNSL
                     YYLFYPAGGVPEHLAYSTAPSITGPWTYGDTIMHVIQKGGAFTNHPGVIDYKGKTYLF
                     YHNGALPGGGGFKRSVCVEPLVFNADGSVPLIEPTQLGVKESASLLNPYTRVQAETIA
                     FSEGVKTLSDSKTGVYVTKIESGDYIKVRSVDFGKGAKKFEASVASASKGGNIEIRIG
                     GVDGELLGTCNVGNTGGALSWSTVQCKVKKVKGIHDLYFVFKGEQGELFNFDWWKF"
BASE COUNT          727 a          639 c          504 g          758 t
ORIGIN      
        1 catttatatt ttttgccgtg gaataacata tctgttctgc ttgaacacct tgaccaacta
       61 ttgaaaccga agttgagtcg ccattaaggt gttgaatctg ctcgaaacgc cagtggacaa
      121 gaacttcact ttgacctgaa acagaaacag aaataccgga aaataaaaaa gctcccagaa
      181 tgcagccaaa tgtatctttg aaaatccgaa tcatcgcttt atatatttgt attctaatta
      241 attaaacgcc caccaatcaa aattgaacaa ctcaccttcg ccacctttga ataccaagca
      301 gatgtcttgt acacctgtcg cttttttcac cttgccttta acggtagccc acttttgcaa
      361 accgccggta tttttcacgt cgatagtgcc caacaattca ccattgcgat cgcccaaacg
      421 gatttcaatt ttcccacctg ctgaggccga agcaacactg gcttcgaatg ttttcgctcc
      481 ttttgcaaaa tcgacactgc gcactttcag atagtcgcca ttctcaattt tagttacgta
      541 aacgcctgtt ttgctgtcag acgctgtttt caagccttcg gaaaaggcaa tggtttcggc
      601 ttcaactttt ttgtagggat ctaaattcgc cacacttttt gtaacgccct ctttggttgg
      661 agtgatgagc ggaatgctgc catcggcatt gtatgtgaat tgctcaacgc tcaccgaacg
      721 cttaaaccct tgtcctttcg acaagtcttg atcgtgataa aaaatgtacg aatttccttt
      781 gaaatcgata attccggcgt gattggtaaa cgccaaatga tcggcacgtt tcataatttg
      841 acctttataa gtccaaggtc cttcgggtga atttgaagta gaatatgaaa tgtactcggg
      901 aataccttcg gaggcataca tcacatagta caagttattg cgcttgaaaa accaaggacc
      961 ttcggtgtaa gttgtaccgg gtcttccgtc gcggccagct ttagagccaa aagactgagg
     1021 tgtcatttcg attgatacaa ttccattcgc accaacactt ttatcgtatg aaatcatatc
     1081 ttcattcaac ttcacatacc acaaacttgg atttccccaa tacaagtacg cttgtccttt
     1141 ctcatcgatt gcaacagtgg ggtcaatatc ctgccaaatg tgatcactgt cgatcaaacg
     1201 tttaccaatt gggtcggtga acggacctgt tggagaatcg gaaaccaaca cggcaattcc
     1261 ttcgccatga atgggcacat acaggtaaaa cttgccgtta cgctcgatac actgaggagc
     1321 ccaagcacca ttgtccttgt tcatccatgt gaaatttttc aatgaagcta ctgctccgtg
     1381 gtcagtccag ttcaccatat ctttcgacga atagcagcgc cagtcgtaca tggtgaaaaa
     1441 attattcacg gttgaatctt cgtcatgcga agtatataaa tagacagtcc cgtcgtacac
     1501 catgggtgcc ggatcggctg tgaaataagt ttgaacaatt gggttttgag ccgacaaact
     1561 accggcaaaa gccacgacaa tcaataagat gtaacttact tttttcattg ctaacaatat
     1621 attttaacac taaaatttcc accaatcaaa attgaacaac tcgccttgct cccctttgaa
     1681 aacgaaatag agatcgtgga tgccttttac tttcttcacc ttgcactgta ctgttgacca
     1741 gcttaatgca cctcctgtat ttccaacatt gcaagttcca agcaattcgc catcaacacc
     1801 tccgattcga atttcgatgt ttccaccttt tgaagccgag gcaacactcg cctcaaactt
     1861 tttggctcct ttcccaaaat caacactgcg cactttgatg taatcgccac tttcaatttt
     1921 ggttacatat acaccggttt tgctgtcact caacgttttt accccttcgg aaaaagcaat
     1981 ggtttcggct tgcactcgtg tgtaaggatt gagcaaggaa gcactttctt ttacacctag
     2041 ctgcgttggc tcaatcaatg gaaccgaacc atcggcattg aaaacaagtg gttccacaca
     2101 aaccgagcgt ttaaaaccac cgccaccggg caaggcacca ttgtggtaga acaaataagt
     2161 tttgccctta taatcaatca cgccgggatg attggtaaaa gcaccccctt tttgaattac
     2221 atgcatgatc gtatcgccat aagtccaggg gccggttatg cttggagccg ttgaataggc
     2281 caaatgctcg ggaacaccgc cagctgggta aaacaaatag tacaggctgt tgcgtttgta
     2341 caaccaaggg ccttcttcgt aagctgcggt acggttgtct atctttttgg cacggtaacc
     2401 aaagttttca tcttttaggt cttccttctt tataccataa gtctcatcaa acgaaatcat
     2461 gtcttcgttt agctttacat gccaaaggtt tgggttgccc caatacaaat acgcttgtcc
     2521 gtaatcgtcg atgaaaacgg ttgggtcaat gtagccataa ccagtcaaaa gtggttttcc
     2581 aagagcatct ttgaatagac cttccggttt ctctcccaca gcaacgcc
//
LOCUS       DAOPUW010000255         2624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_40916_length_2624_cov_2.215259, whole genome shotgun sequence.
ACCESSION   DAOPUW010000255 DAOPUW010000000
VERSION     DAOPUW010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40916_length_2624_cov_2.215259"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1784
                     /locus_tag="PKJ43_05215"
     CDS             <1..1784
                     /locus_tag="PKJ43_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="STN domain-containing protein"
                     /protein_id="HNZ71999.1"
                     /translation="GMRTFFILILLLLMIWPEKSHAQQVPVFERKISVEMENETIEAI
                     LTEISRQSQVSFSYNPELLNASDKKSLSLTKQTVRFALSNLFDGKVNYRERGKFIILQ
                     PKKENSPSVVEGYLTNENGQLLSDATIYNADKKQVATTDQFGYFRLEIDKSKTPDTIK
                     ISKAGFESAMLMPVNNNAPFVQLSLSENQTSLPAEETFYPSILVRRQLRVSSSNIDET
                     FNRFAQLSIAPHLGTNSLLSGSTSNLLSINILGGYTEEVRALELGGVVNIVRRNAGVF
                     QAAGIGNFVGGNFRGCQASGVLNYTRRNFSGFQASGISNTVAGNLTGVQATGIMNYAK
                     NINGTQLTGMINVAGDLYGFQASSILNQAKTVQGAQVTALVNNADSLKGAQITGVLNR
                     ARKIRGTQISGLLNYASEMNGLQLGVVNITNRGRATQIGIININSSGVHNLEIYATET
                     FLTNIAYRGGSKHFHTILVGGIDPGTLGDRLLYTYGFGVGTSFGNRPKLYYDAEVLSQ
                     QITYGNVMGDINMLYKLGFSVNYKVSKSMAITFGLSHNIYVVDTSTPEFETIFSQIPP
                     YFLSNESAGTNRNLKTWVGFKAGIRLF"
     gene            1821..2411
                     /locus_tag="PKJ43_05220"
     CDS             1821..2411
                     /locus_tag="PKJ43_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72000.1"
                     /translation="METLVAEKMKTAFDQYFALPLLFWEAIANAGEIIQCESEFIVKK
                     SHSTEKYMRFIIKGSGGILLWNKNNFVCTDMVLDNDFLSDYFSLLTRQASPCEVITFE
                     ETTFFQINYNELIKITESSPWGDKFWRYATTALYVEKQGHYIQTVTQTATEVYRRIMT
                     YEPGIIQRIPQKFIASYLGITPQSLSRIRKNDSSRL"
     gene            complement(2423..>2624)
                     /locus_tag="PKJ43_05225"
     CDS             complement(2423..>2624)
                     /locus_tag="PKJ43_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72001.1"
                     /translation="LLWARQMNNNLLSYEIFSEDDYALVYWIVVLRNLAVIQFLIISS
                     TCGDIVSSEFMYCFIIINRLSG"
BASE COUNT          823 a          579 c          545 g          677 t
ORIGIN      
        1 taggcatgag aacatttttt atcctcattc ttctgttact tatgatttgg cccgagaaaa
       61 gccatgcaca gcaagtacct gtttttgagc ggaaaatatc ggtcgaaatg gaaaatgaaa
      121 ccatcgaggc catacttacc gagataagca gacagtcgca ggtgtcattc tcttacaatc
      181 ctgaattact gaacgcttca gataagaaat cattgtcgct taccaagcaa accgttcgct
      241 tcgccctgtc gaaccttttc gatggaaaag taaactaccg ggaacgtggc aaatttatta
      301 tccttcaacc caagaaagaa aattccccgt cggttgttga aggttacctg accaatgaaa
      361 acggacaact gctatctgac gcaacaatat ataatgctga caaaaagcaa gttgccacca
      421 ctgaccaatt tggatatttt cgcctcgaaa ttgacaaaag caaaactccc gatacgatca
      481 agatcagtaa agcaggtttc gaaagtgcca tgctaatgcc agtgaataac aacgccccat
      541 ttgttcaact ttcgttgtcg gaaaaccaga ctagcttacc tgccgaagaa accttttacc
      601 cgtcgatact ggtgcgtcgc cagttacggg tcagctcgtc gaatatcgac gaaacgttta
      661 accgctttgc ccagctgtca attgccccgc atttaggaac caatagttta ttgtcgggat
      721 cgacctctaa cttattatct ataaacatac taggaggtta tactgaagaa gtcagggcat
      781 tggaattagg tggagtggta aatattgtac gtcgaaatgc cggtgtcttt caagccgctg
      841 gtattggaaa ttttgttggg ggaaactttc gcggatgcca ggcttcggga gtactaaact
      901 atacgcgtcg caacttttcg ggctttcaag cctcggggat atcaaatacc gttgccggga
      961 atcttacagg agttcaagct acaggaatta tgaattacgc caaaaacata aacgggactc
     1021 aacttacggg aatgatcaat gttgccggcg atttgtacgg atttcaggct tcctcaattt
     1081 taaatcaggc caaaacagtg caaggagccc aggtaacagc attggtaaat aacgcagatt
     1141 cgctcaaggg cgcccaaatt acaggggtgc taaaccgcgc gagaaaaatc aggggaacac
     1201 agatatcggg gttgttaaac tatgccagcg aaatgaatgg tttacagtta ggagtagtga
     1261 atatcacaaa tcgtgggcgg gcaacgcaaa ttggtatcat taatattaac agtagcgggg
     1321 tgcacaacct cgaaatatat gccaccgaaa catttttgac caatattgcc taccgtggag
     1381 gatcgaaaca cttccatact atccttgttg gcggaatcga ccctggcaca ttgggagaca
     1441 gattacttta cacctatggg tttggcgtag gaacctcgtt tgggaaccgc ccgaaattgt
     1501 attacgacgc cgaagtcctc tctcagcaaa ttacctatgg aaatgttatg ggagacatta
     1561 atatgttata taaattgggt ttcagcgtca actataaagt ttcgaaaagc atggcaataa
     1621 cctttggcct tagccacaac atttacgttg ttgacacctc aacccctgaa tttgaaacca
     1681 ttttcagcca aatcccgcct tactttttat cgaacgaatc tgcaggaacc aaccgaaact
     1741 taaaaacctg ggtaggattt aaggccggga taagattgtt ctaggcaaaa gcaaacacta
     1801 catctcgcaa aatggacaat atggaaacat tggtggcaga gaaaatgaaa accgcatttg
     1861 atcagtattt tgccctcccg ctactgtttt gggaagccat agccaacgcg ggcgaaatca
     1921 ttcaatgtga aagtgagttc attgtaaaaa agagccacag caccgaaaaa tatatgcgct
     1981 ttattatcaa ggggagtggc ggcatactcc tttggaataa aaataatttt gtttgtaccg
     2041 atatggtgct cgataacgat ttcctgagcg attacttctc gctgctcacc cggcaggcat
     2101 cgccctgtga agtaataacc tttgaagaaa caaccttttt ccagatcaat tataacgaat
     2161 tgattaaaat aaccgagagc agtccctggg gcgacaagtt ctggcgctat gccaccactg
     2221 cactctatgt agaaaagcag ggacattata tacaaaccgt aacgcaaaca gccactgaag
     2281 tttaccgccg cattatgact tacgaaccag gtattattca acgcattcca caaaaattta
     2341 tcgcttcgta cctcggaatt acccctcaaa gtttaagccg gatacgaaaa aatgattcat
     2401 cccgattgta atccttattg aattatcctg aaagtcggtt tattatgata aaacaataca
     2461 tgaactctga ggaaacgata tctccacatg tacttgaaat tattagaaac tggattaccg
     2521 caagatttcg caaaacgaca atccagtaaa ctaacgcata atcatcctcg ctaaaaattt
     2581 cataggacaa caaattgttg ttcatttgcc tcgcccacaa cagt
//
LOCUS       DAOPUW010000256         2596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_41561_length_2596_cov_2.844943, whole genome shotgun sequence.
ACCESSION   DAOPUW010000256 DAOPUW010000000
VERSION     DAOPUW010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41561_length_2596_cov_2.844943"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PKJ43_05230"
     CDS             <1..431
                     /locus_tag="PKJ43_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72002.1"
                     /translation="VDETLRNTIDVIQLKNNFYLLAQQLNNEGKNDKARQVIEKLYSI
                     LPVGKYEVSYYDILISSVWYSLSDTNKGDELMKTIAASGFQKIDFYLSLGSDFVKSYQ
                     REINREISIMKELIRVAEFYKRQDLIKEIDQKLSSITGKV"
     gene            complement(502..1227)
                     /gene="recO"
                     /locus_tag="PKJ43_05235"
     CDS             complement(502..1227)
                     /gene="recO"
                     /locus_tag="PKJ43_05235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNZ72003.1"
                     /translation="MITSTKAIVLHQVKYAETSIIVTLYTEAFGRQSYIVNGIRSAKS
                     KQKMGLLQPLFQLEIEAYHKPGRGVQRIKEFRADYVYQSVPFDVTKRSVSLFLAEVML
                     KVLRSEESDDEAFIFIQNSLQFFDQMAKGSANFHLWFIVNLLKYLGLQPNNNFSETNR
                     WFDMKNGRFLITRPLLPQTPDAESSRLLSVLFSHSLDTLHQLELNGKQRTRLLELLLE
                     YYAIHFEHIGRINSLDILKELFY"
     gene            complement(1224..>2596)
                     /locus_tag="PKJ43_05240"
     CDS             complement(1224..>2596)
                     /locus_tag="PKJ43_05240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ72004.1"
                     /translation="ENGIYTQLTPQGPFDNNVLSLSFSDGTLWAASGGRNTAWGNMFF
                     DFNVNAYTDGKWKWYNQSTGAVDPGFYDAIQVLPVPGDPNHFFVSTLGFGILEYENGS
                     LKKIHNDQNSSLQNIFPGDNYVRIGGMAFDSKGNLWASNSEVARSLHKYSPDGKWQGF
                     VLEEIMNTYDLGSLLVTQNDDIWMVIPRNKTRGIYVMSNDGTQTKHLDVSSLFIGRDN
                     NNKEVTVITSMNDVYSLAQDLDGEIWVGTSNGVNVYSQPENVFSEEKFYGNRPSVDYN
                     DGIFHPLLENFTVTSIAVDGGNQKWCGTRGNGLFLISDDGEQELEHFTTENSPIISNN
                     INSLAFDGTTGMLYIGTDMGIVSYRTKSNTSNSKFRKVYAYPNPVRENYTGDIFITGL
                     VYNTNVKITTTSGRLVYETTSEGGQAVWNGKDLAGNRVHTGVYLAFCSSPDGNQSAVT
                     KILFIR"
BASE COUNT          803 a          551 c          495 g          747 t
ORIGIN      
        1 tggtcgatga aacgttgaga aacacgatcg atgttattca attgaaaaac aatttctacc
       61 tgcttgccca acagctcaat aacgaaggaa aaaatgataa agcaaggcaa gtcattgaaa
      121 aattatactc gattttgccg gtcggaaaat acgaagtttc gtattacgac atccttattt
      181 cttcagtttg gtattcattg tcagatacga acaaaggtga tgaactgatg aaaaccattg
      241 ctgcctcggg tttccaaaaa attgatttct acctgtcact tggttccgat tttgtaaaaa
      301 gctaccaacg cgaaattaac cgtgaaatct cgattatgaa agaattaatc agggttgccg
      361 aattttacaa acggcaagat ttgataaaag aaatcgatca aaaactttca tcgattactg
      421 gaaaagtata gcatatttta tcaaacaatt tctcgaaagg ttgtcggaat ttcccgataa
      481 ccttttttta tgctgatttt ctcaatagaa caactctttt aaaatatcga gagaattaat
      541 tcttccaata tgctcgaaat gaatggcata atactcaagg agtagttcaa gcaaacgggt
      601 tcgctgcttt ccgttcagct ctaattgatg gagcgtgtca agcgaatgag aaaataaaac
      661 cgacaataac cgggaagatt cagcatcagg tgtttgcgga agaaggggcc gcgtaatcaa
      721 aaaacggcca ttcttcatat cgaaccaccg attggtttca ctaaagttat tattcggttg
      781 caagccaagg tatttcaata agttgacgat aaaccacaag tgaaaattag ccgaaccttt
      841 tgccatttgg tcaaaaaatt gaagcgaatt ctgtataaaa atgaaggctt catcgtcgct
      901 ctcttcgctc cgcaacactt tgagcataac ttcggccaaa aacaacgaga cggaacgctt
      961 ggtaacatca aaaggcacag actgatacac ataatcggca cgaaactctt ttatccgttg
     1021 aactccacgg cccggtttgt ggtatgcttc aatttcgagt tgaaacaagg gctgtaacaa
     1081 gcccatcttt tgtttcgatt tcgcacttct gatgccattc actatatacg actggcggcc
     1141 aaatgcttca gtgtataaag taactattat actggtttcg gcatatttaa cctgatgtaa
     1201 aacaatggct tttgtgctgg ttatcatcgt atgaaaagga tttttgttac ggccgactga
     1261 ttaccgtcgg gcgaagaaca aaaagcaaga taaacgccgg tatgaactcg gtttccggcc
     1321 aaatctttac cattccaaac cgcttgtccg ccttccgaag tggtttcata aaccaaacgt
     1381 ccgctagtgg tagttatctt cacattcgtg ttatatacta aaccagtaat gaaaatatcg
     1441 cctgtgtaat tttcccgaac cggatttggg tatgcataca cttttctgaa ttttgaattc
     1501 gaagtatttg attttgtgcg atatgacaca atccccatgt cggtgccaat ataaagcatg
     1561 ccagtagtcc catcgaaagc caggctatta atattgtttg aaataattgg tgaattctct
     1621 gtagtgaaat gctcaagttc ctgttctcca tcgtcagaaa tcaggaacaa tccgttgcca
     1681 cgtgtaccac accatttctg attcccccca tcgacagcta tggaagttac cgtaaagttc
     1741 tcgagcagag gatgaaaaat gccatcatta taatcgacac ttggacgatt accgtaaaat
     1801 ttttcttcgc taaaaacatt ctctggctgg ctataaacgt ttaccccatt tgaggttcca
     1861 acccaaatct cgccatcgag gtcctgagcc agagaataca catcattcat cgaagtaatt
     1921 actgtgactt ctttattatt attgtcccgg ccaataaaca acgacgaaac atcgaggtgt
     1981 tttgtctgcg ttccatcatt gctcatcaca taaattccac gggttttgtt tcgtggaata
     2041 accatccaga tatcatcatt ttgtgtaacc agaagagaac ccaaatcgta tgtattcata
     2101 atttcttcaa ggacaaaacc ttgccatttg ccatcgggag agtatttatg aaggctgcgt
     2161 gcaacttccg aattgcttgc ccaaagattt cctttagaat cgaaagccat cccgccaatc
     2221 ctcacataat tgtcgcccgg gaaaatattt tgtaagcttg aattctggtc attatggatt
     2281 tttttcaatg aaccattttc atactcaaga attccaaacc caagggtgga tacaaaaaaa
     2341 tgattggggt ctccgggaac aggaagaacc tgaatggcat cgtaaaagcc gggatcaacg
     2401 gcaccggtcg attggttata ccacttccat tttccatcag tataagcatt tacattaaaa
     2461 tcgaagaaca tattgcccca agccgtattt ctacctccag atgcagccca aagtgttccg
     2521 tcggaaaacg acaatgaaag cacgttattg tcgaacggtc cctgaggggt aagctgtgta
     2581 taaattccat tctcat
//
LOCUS       DAOPUW010000257         2591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_41702_length_2591_cov_2.725158, whole genome shotgun sequence.
ACCESSION   DAOPUW010000257 DAOPUW010000000
VERSION     DAOPUW010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41702_length_2591_cov_2.725158"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PKJ43_05245"
     CDS             <1..210
                     /locus_tag="PKJ43_05245"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNZ72005.1"
                     /translation="SDEKYCLFGKGGGEFLANEFDIPLLAQIPLTERLCDNCDEGKLN
                     ELLSDSAVKKSFDLLLEGILNSKHD"
     gene            203..625
                     /locus_tag="PKJ43_05250"
     CDS             203..625
                     /locus_tag="PKJ43_05250"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNZ72006.1"
                     /translation="MIEHQYSFRVMYPDTDQMGTVHHSNYPKYYETARWELFRSIGIS
                     YKSVEEAGYMLPVVDMKFKFLKTTHYDALLTVKTTLKAIKGVRIWFTYQLYNEQNELI
                     NEAETELAFVKRDNWKPCAAPDFVLKAIEEHETKQKAC"
     gene            619..1083
                     /locus_tag="PKJ43_05255"
     CDS             619..1083
                     /locus_tag="PKJ43_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HNZ72007.1"
                     /translation="MLTKEEIEKRTDQAVDFFEAGYNCAQSVLLAYADLYGLDNEMAA
                     KMATSFGGGMGRLREVCGAVSGMFMVLGFEYPFIDTKDKASKNENYKKVQETAAEFKA
                     SMSSIICADLLSLKREAQHYESAERTKEYYATRPCAKCVAIAAEITGKNITK"
     gene            1080..1460
                     /locus_tag="PKJ43_05260"
     CDS             1080..1460
                     /locus_tag="PKJ43_05260"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNZ72008.1"
                     /translation="MKKIAVPTRGNAVDDHFGHCEMYTVFTIGKDKKIENSEIVPSPQ
                     GCGCKSNIASVLKEMGVTTLLAGNMGDGALNVLNCNGIQVFRGCKGDVRQVTETYLKG
                     YILDSGEGCHSHEHHGEEGHVCSH"
     gene            1586..2377
                     /locus_tag="PKJ43_05265"
     CDS             1586..2377
                     /locus_tag="PKJ43_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ72009.1"
                     /translation="MKIITLIENVVYGQGLIAEHGLSLYIESQNQKILFDTGQSGLFL
                     QNARALGIKIEEIDSVVLSHGHYDHTGGLYPFLEANPKAKLYAKRALFDTKYRNNRTF
                     IGTPKNDELLKDRLVYVDEITELDEGVFIMPHIPIKHQIDKHFDGLLKLKDNELVPDE
                     FDDELYLSLVENNLVNIVSACSHRGITNICEAATAHFNLRVGLILGGFHIKNCTTEQY
                     IHLLIISEHCNPSSLEFVTALESKSMPTFAKNSKYPCFTTIQGTR"
BASE COUNT          843 a          566 c          555 g          627 t
ORIGIN      
        1 tccgacgaaa aatattgcct ttttggtaaa ggcggcggag aatttttagc taacgaattc
       61 gacatccctt tgcttgccca aataccgctt accgaaagac tttgcgacaa ttgcgacgaa
      121 gggaaactga atgaactgct gtccgacagt gcagttaaga aaagttttga tttacttttg
      181 gaaggaattt taaactcgaa acatgattga acatcaatac agctttaggg tcatgtaccc
      241 tgatactgac cagatgggta ctgtacatca ttccaattac ccaaaatatt acgagactgc
      301 ccgttgggaa ctgttccgca gcattggcat ttcttacaaa tcggttgaag aggctgggta
      361 tatgctccca gttgtagata tgaaatttaa gttcttaaaa acaacacatt acgatgccct
      421 tctcactgta aaaaccacat taaaagccat caaaggtgtc cgtatatggt ttacctacca
      481 actttataac gaacaaaacg agctgatcaa cgaagcagag accgaactgg cttttgtgaa
      541 acgcgacaac tggaaaccgt gtgccgcccc cgattttgtg ttaaaagcga ttgaagaaca
      601 cgaaacaaaa cagaaagcat gttgacaaaa gaagaaatag aaaaacgaac cgatcaggca
      661 gtcgatttct ttgaagcggg ctacaattgc gcccaatcgg tgctgctggc ctacgccgat
      721 ttgtatggcc tcgacaacga aatggcggca aaaatggcca catcgtttgg cgggggaatg
      781 ggccgtttgc gcgaagtgtg cggtgccgta agcggcatgt ttatggtgct ggggttcgaa
      841 tatcccttca ttgacacaaa agataaagca tcgaaaaacg agaactataa aaaagtacag
      901 gaaaccgccg ctgaatttaa agcaagcatg agctcgatta tttgtgccga tttgctaagc
      961 ctgaaacgcg aagcacagca ctacgaatcg gccgaacgga ccaaagaata ttatgccacc
     1021 aggccatgcg ccaaatgtgt ggcgatagca gcagaaataa caggaaaaaa cataacaaaa
     1081 tgaagaaaat agcagtaccc acacgcggaa atgcagtgga cgaccacttt ggccattgcg
     1141 aaatgtacac cgttttcacc attggtaaag acaaaaaaat tgaaaattca gaaattgttc
     1201 cttcgccaca aggttgcggt tgcaagagca acattgcctc ggtgctgaaa gagatgggcg
     1261 taaccaccct gcttgccgga aacatgggcg atggggcttt gaacgtactg aactgtaacg
     1321 gcattcaagt atttagaggc tgcaaaggtg atgtacgcca agtaaccgaa acgtacttaa
     1381 aaggctacat tctcgactcg ggcgaagggt gtcacagcca cgagcatcat ggcgaagagg
     1441 ggcatgtgtg cagccactag attgtacttt tataaaaaaa atgaaacaca aagcaaacga
     1501 aagatatcac taagaactct aatatattca ctttgtgatc tttgtgttaa cccatagtgt
     1561 acttcgtgtt taaactaaac gaacaatgaa gataattacc ctgatagaga atgtggttta
     1621 cggccaaggc ttgattgccg aacacggcct ttcgctatac atcgaaagcc aaaatcagaa
     1681 aattctgttc gacactggcc aaagtggatt atttcttcaa aatgcccgag cacttgggat
     1741 taaaatcgaa gagattgaca gtgtagtgtt atcgcacggc cactacgacc atacaggtgg
     1801 actttaccca tttctggaag ccaacccaaa agcaaaactg tatgccaaac gggctctttt
     1861 cgacaccaaa taccggaaca acagaacctt tatcggcaca ccgaaaaacg atgaactact
     1921 gaaagaccgt ctagtttatg ttgatgaaat cactgagctg gacgaagggg ttttcattat
     1981 gccccacatt cccataaaac accaaataga caaacatttc gacgggttac tgaaattgaa
     2041 agacaacgaa cttgtacccg atgaatttga tgatgaactt tatctttcgc tggttgaaaa
     2101 caatttggta aatatcgtgt cggcctgctc gcaccgggga attaccaaca tttgtgaagc
     2161 cgcaaccgct catttcaatc ttcgcgtggg tttaatactg ggcggctttc atataaaaaa
     2221 ctgcactacg gagcagtaca tccacctact cattatttcc gaacactgca atccaagcag
     2281 cttggagttt gtcactgcac tggaatcgaa aagtatgccg accttcgcca agaactcgaa
     2341 gtacccctgt tttacaacca tacagggcac gaggtaacta ttacttaaaa ctccgcaatt
     2401 tcagtcaagt ttcttacttc aattcgcaat acttgtcaag agcggaaaaa tccgaacctc
     2461 tatttttgca agcgttaccg gtgacgttca gttttttaat cattaaaaac atgtaaaaat
     2521 ggaaaacaca attcaaatac agcccattgg ccatgtaagt actgccaata aacagttctc
     2581 gataatactt g
//
LOCUS       DAOPUW010000258         2588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_41772_length_2588_cov_3.831030, whole genome shotgun sequence.
ACCESSION   DAOPUW010000258 DAOPUW010000000
VERSION     DAOPUW010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41772_length_2588_cov_3.831030"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2588)
                     /locus_tag="PKJ43_05270"
     CDS             complement(<1..>2588)
                     /locus_tag="PKJ43_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNZ72010.1"
                     /translation="DENGKILITSNSDYIDIYDYPTNSFEHVLITVEKTPNWTNLSKS
                     IKLDKDGNALITTRGLGIIKWDRKTGVFSPITLTDEQGKPIELKIIRSSLIDRTNKIW
                     IGTDGNGLVVYDSEKNQAKHYPGTPGDPSQLTRGAIYSLYEDQEGNIWIGTYVAGLNK
                     MVPQKQNFGLFVSNPAIKNGLSHSCVTSFCEDSEKKIWIGTDGGGLNCFDPATGKFTH
                     IKKDLADKDGLTVNVVMALYCDPEDNIWIGTYNGGLNIYNQKTKKFRHYRFNADDTTT
                     ISSDHPWGFVRDRWGNMWVATVNYGLNLMKPGESKFIRYGIANSATSGFIQLMSEALT
                     HMFIDSKDRLWIGSEWGLEMVELANVDFSKPKPELVFKHFLQSDSVNSISDNRISYIN
                     EDRFGNIWVGTKGGGINILDTKTWLFTQYSTGQGLPHNIVDGILFDNNDNAWISTNNG
                     LSVFNPVSKQFKNYTTSDGLQSNVFFKTASFKTNDGMFYFGGIKGFNAFRPENVTGIQ
                     TELTTSIINLKIFNKEVRVGDVFGKRIILEKPIGQTDAIHLTYKEKEISFEFSALNYI
                     NPVKIHYAYKLEGFNNDWQTTDAQMRVAKYTNLNPGEYTFCVKATYNEDEWPEKATTL
                     KIIIDPPWWKTLPFMFLVFVLSVVLVLGFFYLRLSSYHKQQRILEKAVDEKTIQLRVA
                     NQQLTEANVTKDKFLSIIAHDLINPFSSIIGFSDLLLSNYWEWDDKARLDALKTIGHS
                     SEQLYELLGNLLQWSRSERNTLKCNPERVNISQNIRKIVELLKVTADAKELTIEKYLD
                     EENYFVYSDIQLLNTVIRNLLSNAIKFTPNGGKIQIRTEKNEGFLVVSVIDNGVGMSE
                     NDISKL"
BASE COUNT          747 a          536 c          448 g          857 t
ORIGIN      
        1 aagtttacta atatcgtttt ctgacatccc aaccccattg tcaataacag atactacaag
       61 gaacccttca tttttttcgg tccgtatctg aatttttcct ccatttgggg tgaattttat
      121 ggcattactc agcaagtttc taattacagt gttcagcaat tgaatatcgc tgtatacaaa
      181 atagttttct tcgtcgagat atttttcaat tgtaagttcc tttgcatcag ctgtaacttt
      241 cagaagctcg acaattttac gtatattttg acttatgttt accctttccg gattgcattt
      301 taaggtattc ctctcagaac gggaccattg aagcagattt ccaagcaatt catatagttg
      361 ttccgatgag tgtccaatgg tttttaaggc atcgagacgt gccttatcgt cccattccca
      421 gtaatttgaa agtaataaat cagaaaaacc aatgatactg ctgaaagggt taataaggtc
      481 gtgggcaata atggaaagaa acttatcttt agtcacattt gcctccgtaa gttgctgatt
      541 tgcaacacgc aattgaatcg ttttttcatc aacagccttt tctaatattc tttgctgttt
      601 atgataagaa ctcagacgta agtaaaaaaa acctaaaact aagacaacgg aaagcacaaa
      661 gaccaagaac atgaaaggta gagttttcca ccatggcggg tctatgataa tttttaatgt
      721 agtagctttt tcaggccact catcctcgtt gtaagtcgct ttaacacaaa aggtatattc
      781 tcccggattc aggttagtat attttgccac cctcatttgg gcatcggttg tttgccaatc
      841 gttgttaaag ccttcgagtt tgtatgcata atgtatttta acagggttaa tatagttgag
      901 ggctgaaaat tcgaaagaaa tttctttttc cttgtaggtt aaatgaatag catcggtttg
      961 ccctattggt ttttcaagaa ttatccgttt cccgaaaaca tcaccaaccc gcacttcttt
     1021 attgaaaatc tttaagttaa taatagatgt tgtaagctct gtttgaattc ctgtaacatt
     1081 ctccggacga aatgcattga aacctttgat ccccccaaaa taaaacatcc catcgttggt
     1141 cttaaaactt gccgttttga aaaacacgtt agattgtagg ccatcagacg ttgtataatt
     1201 tttaaattgc tttgaaacag ggttaaaaac cgacaacccg ttatttgtgc taatccacgc
     1261 attatcgtta ttatcgaaca atatcccatc aacaatgttg tgtggaagtc cctgacctgt
     1321 tgaatattgt gtgaaaagcc acgtttttgt gtcgagaata ttgattccgc cacctttggt
     1381 accaacccaa atattcccaa accgatcttc attaatgtag cttatcctat tgtcactaat
     1441 gctgtttact gaatcggact gtaaaaagtg tttaaaaacc aattctggtt tcggcttgga
     1501 aaaatcaacg ttggcaagtt caaccatttc caagccccat tcagaaccta tccataagcg
     1561 gtctttacta tcgataaaca tgtgagtcaa tgcttcactc attaattgga tgaaaccaga
     1621 agtagcagaa ttagcaattc catatctgat aaatttcgat tcgccgggct tcataaggtt
     1681 tagcccataa tttacagtcg ccacccacat atttccccat cggtcacgaa caaaacccca
     1741 agggtggtcg ctgctaatag tagttgtgtc atcggcatta aagcgataat gcctgaactt
     1801 ttttgttttc tggttgtaga tgttaagtcc cccgttataa gtaccaatcc atatattgtc
     1861 ttctggatca caatataaag ccatcaccac gtttactgtt agcccatctt tatcggcgag
     1921 gtctttttta atatgagtga atttcccggt tgccgggtca aaacaattta acccgccacc
     1981 atcggtgcca atccatattt ttttctcaga atcttcgcaa aaactggtca cacaactgtg
     2041 acttaagcca tttttaattg ccggattact aacaaaaagc ccaaaattct gtttttgtgg
     2101 aaccattttg tttaatcctg ccacataggt gccaatccaa atatttcctt cctgatcttc
     2161 gtacaaagaa tagattgcac ctctggtcaa ttgagatggg tctccgggag tgcccggata
     2221 atgcttcgcc tgattttttt ctgaatcgta aacaacaagc ccgttaccat cggtaccaat
     2281 ccatatttta tttgtacggt caatcagtga cgagcgtata attttcaatt caatgggttt
     2341 tccctgttca tcggtaagtg ttattggcga gaaaacaccg gttttcctat cccatttaat
     2401 tatgcccaag ccccttgtag taataagagc attaccatcc ttatcaagct ttatgctttt
     2461 tgaaagattg gtccaattag gtgttttctc aacagtaata agcacatgct caaaactatt
     2521 tgttgggtaa tcataaatat cgatgtaatc agaatttgat gtaatcagaa ttttcccgtt
     2581 ttcgtcct
//
LOCUS       DAOPUW010000259         2583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_41907_length_2583_cov_3.146361, whole genome shotgun sequence.
ACCESSION   DAOPUW010000259 DAOPUW010000000
VERSION     DAOPUW010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41907_length_2583_cov_3.146361"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..2086)
                     /gene="ligA"
                     /locus_tag="PKJ43_05275"
     CDS             complement(77..2086)
                     /gene="ligA"
                     /locus_tag="PKJ43_05275"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNZ72011.1"
                     /translation="MENNTVQQQIEELRSKLHHYNYLYYVQSQPEISDFEFDSLMKQL
                     EALENENPELDDPNSPTHRVGSDIISEFKQVTHKYSMLSLSNTYNEGELRDFDNRVRK
                     DINQEPEYVCELKFDGVSISLIYENGMLKHAVTRGDGEQGDDVTANVRTIKSIPLKLR
                     GEGWPAEFEIRGEIVLPYEAFNKLNEERLANGEEPMANPRNTTSGTIKMQDPSIVARR
                     GLDAYFYFVPSQIRLTESHSQNLMLAASWGFKTSEHTRVCKNIDEVLNFIHYWDKERH
                     NLPVATDGVVIKVNSIAMQNNLGTTAKSPRWAVAFKFQAEQAETRLLSVSYQVGRTGA
                     ITPVANLEPVLLSGTVVKRASLHNADIISSLDLRINDIVKVEKGGEIIPKIVGVNQVE
                     RHPMNQPVMFINVCPECGTPLVRDEGEAKHFCPNENGCPPQIKGKIIHFIGRKAMNID
                     GMGEETVELFYNTGLIRNVADLYTLQISDMAQLERLGEKSARRIADGLQASTQVPFER
                     VLFSLGIRFVGETVAKILVKSFKTIDALLAATLDELTQVNEIGSRIAQSITDWFSIED
                     NRILIDRLRNVGLQFEMSEDQMAGSSTKLAGLSIVISGTFAQHSRDELKELIEKHGGK
                     NVGSISKSTSYLLAGENMGPSKLEKAQKLAIKIISEDEFMTMISE"
     gene            complement(2206..>2583)
                     /locus_tag="PKJ43_05280"
     CDS             complement(2206..>2583)
                     /locus_tag="PKJ43_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72012.1"
                     /translation="KTLRGVSRNRVVSDGFVYGNLELRWKPVHFRFINQNFYIGLNAF
                     LDGGQAIQKIDIQDKIKNIDFGQETEADYFNLSGNEKLHLSYGAGLRIVMNENFVISC
                     DYGRAIDPQDGKSGLYIGLNYLF"
BASE COUNT          725 a          563 c          482 g          813 t
ORIGIN      
        1 tcctgtaaat attttgttcc tgaaacagcc attaaccaca tagtgttgac atacagtatt
       61 ttaatgaaat tttaaatcat tcggaaatca tcgtcataaa ttcatcctca gaaatgatct
      121 taatggccag cttttgtgct ttctcgagtt tacttggacc catattctca ccggcaagca
      181 ggtaacttgt acttttcgaa attgacccca cattttttcc gccatgtttc tctatgagtt
      241 ctttcagctc atcgcgcgag tgttgggcaa aagttcccga aatcacaata gatagtccgg
      301 caagtttagt cgatgaaccg gccatttggt cttctgacat ttcgaattgt agtcctacat
      361 ttctcagacg atcgattaat attcggttat cttcaattga aaaccaatcg gttatgcttt
      421 gggctatgcg acttccaatt tcattaacct gagttaattc atcaagagta gctgccaata
      481 aagcatcgat agttttaaaa cttttgacca gaatcttagc gacagtttcg cctacaaacc
      541 taattcccaa cgaaaagaga accctctcaa aaggaacttg ggttgaagcc tgaagcccat
      601 cagcaatacg ccgggccgat ttctcaccca agcgttccaa ttgggccata tctgaaatct
      661 gtaatgtata caaatcggca acattcctga taagtcccgt attataaaat agttcaacag
      721 tctcctctcc catcccgtct atgttcatag cttttcgccc tataaagtgg attattttac
      781 cttttatttg aggaggacaa ccattttcat taggacaaaa atgttttgct tcaccttcgt
      841 cgcgaacaag tggagtaccg cattcgggac aaacattgat aaacattacc ggttggttca
      901 ttggatggcg ctcaacctgg ttaaccccaa caatcttagg gataatttca cctccttttt
      961 ctactttcac aatatcattg attcgtaagt cgagtgatga aataatgtcg gcattatgta
     1021 gcgaagccct tttaaccaca gtaccgctca gcaaaacagg ttcaaggtta gcgacaggag
     1081 taatggctcc tgtgcggcct acttggtacg aaacagacaa aagccgggtc tcggcttgtt
     1141 cagcttggaa cttaaaggca acagcccatc gcggactttt cgccgttgtt cccaaattgt
     1201 tttgcatggc aattgaattc acttttataa cgactccatc ggtagccaca ggaaggttgt
     1261 gacgctcttt atcccaataa tgaataaaat taagtacttc atcaatgttt ttgcaaacgc
     1321 gtgtatgttc cgatgtttta aacccccaag aagctgctaa catcaggttt tgtgaatgac
     1381 tttcggtaag gcgtatctgg ctggggacaa aataaaaata agcatcgaga ccgcgacgag
     1441 ccacgatgga aggatcttgc atttttatgg ttcctgaagt ggtgttcctc ggattagcca
     1501 ttggttcttc accatttgct aatcgttctt cgttcagttt gttaaaagct tcgtaaggta
     1561 aaacaatctc tccacgaatt tcaaattctg caggccagcc ttctccccgg agtttcaacg
     1621 gaatactttt tatggttctt acattggctg ttacgtcgtc gccttgttca ccatcgccac
     1681 gggttacagc atgtttcagc atcccgtttt cataaattaa actaattgaa accccatcga
     1741 atttcagttc acatacatat tctggctcct gattgatatc ttttcgcaca cggttgtcaa
     1801 aatcgcgcaa ttcaccttca ttgtatgtat tactgagcga caacatggaa tacttatgcg
     1861 taacttgttt aaattcagag ataatgtcac tacctacccg atgtgttggt gaatttggat
     1921 cgtcgagttc gggattttca ttttccagag cttcgagttg tttcatcaaa ctatcaaatt
     1981 cgaaatcaga aatttcaggt tgagattgaa catagtagag gtagttataa tgatgaagct
     2041 tgctgcgaag ttcttctatt tgctgttgta ctgtgttatt ttccatggtg aacgttgtgt
     2101 ttactgtgtt aaaaatagct gaaataatat attgaattag caaggacgca aaaaaaaacc
     2161 tttgccaaac aatgacaaag gttttttgct ggtaacatgt tgttactaaa acaagtaatt
     2221 caagcctata tataaccctg attttccgtc ttgcggatca atagcacggc cataatcgca
     2281 tgagattaca aaattctcgt tcatgacaat tcgtaaaccc gccccataac taaggtgtag
     2341 tttttcattt cccgaaaggt tgaaatagtc agcttcagtt tcctgcccaa agtcgatatt
     2401 cttaatttta tcctgaatat caattttttg aattgcctgg cctccgtcaa ggaatgcgtt
     2461 caacccaata taaaagtttt ggtttatgaa cctgaagtgt actggtttcc atcgaagttc
     2521 gagattgcca tacacaaacc catcactcac cacacggtta cgacttaccc cacgaagggt
     2581 ttt
//
LOCUS       DAOPUW010000260         2574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_42123_length_2574_cov_4.013100, whole genome shotgun sequence.
ACCESSION   DAOPUW010000260 DAOPUW010000000
VERSION     DAOPUW010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42123_length_2574_cov_4.013100"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..469
                     /locus_tag="PKJ43_05285"
     CDS             164..469
                     /locus_tag="PKJ43_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72013.1"
                     /translation="MKNKLLIFNKIYVGLLLGLALPVLTFYVYFLIRHHQEISFEFFI
                     SMLHKYRLLFKVVSLCVLVNLPVFYGFIQLKFWQTSKGIVMACFLFAFAVAGYQLFT"
     gene            474..1622
                     /gene="lpxB"
                     /locus_tag="PKJ43_05290"
     CDS             474..1622
                     /gene="lpxB"
                     /locus_tag="PKJ43_05290"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNZ72014.1"
                     /translation="MKYFLIAGEASGDMHGARLMAEVKEYDQKADFMYFGGDLMKEQG
                     GTLLKHYREMAYMGILPVLTHLGAIKRNLTLCKKQLLMFNPDVVILIDYPGFNLRIAK
                     FAKKKKYATAYYISPKIWAWKTRRIKSIKRYIDQMYTIFPFETSFYQQFNYQVNYVGN
                     PVYDIIQEEKEKKIDFQQFCKNNNLSGKPIVALLAGSRKHEVKEMLPIMEQVSDHFSE
                     YEFVIAGVDALGSNFYKETLIKNLKVVYNQTYALLQNSKAALVTSGTATLETGLLYVP
                     QVVCYRMGFGWFLERIKDKVLKTKYFSLINIIAGKEVVKELFQSQVNVQNLSTELDKL
                     LHNTEYQQNMLNNYRSIENTIKTNGAAKAAAQLIVESIKTQKKDDTSA"
     gene            1606..>2574
                     /gene="gldF"
                     /locus_tag="PKJ43_05295"
     CDS             1606..>2574
                     /gene="gldF"
                     /locus_tag="PKJ43_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HNZ72015.1"
                     /translation="MIPLLKKELNTFFGSITGYLVIAIFLLATSLFLWVIPGNFNIID
                     GQQASLSGFFELAPWLYLFLIPAITMRMFAEEKKSGTIEILLTRPISTWGIVLSKFLA
                     SLLLVCISLIPTLLYFWSVARLGNPVGNIDTGATWGSFIGLLFLAAVYISIGLFASSA
                     TSNQIISFLVSLLISYLFYLGFGFIVYTSVSEAFKSFLIEAGINEHYISISRGVIDST
                     DLFYFILTSLLFLFLSGLMIRKQRISVKKILPGLGILGLTIVLVSIPLFRIDLTQEKR
                     YSLSPQTKEIIKNQKYNINIELYLAGDLPAGMRNFQQEIIDKIEDLN"
BASE COUNT          783 a          549 c          505 g          737 t
ORIGIN      
        1 aatgaagact atttctggat cacaggatat taccaaaatc tcgataacgg gagcgacgat
       61 accgacaacc atgcattggc cgaaggctac atttcggtgg ttcccatcct ggtagatatg
      121 acaaaccacc atgcaattga aaaaataaaa aaatggcatt tcaatgaaga ataagctgct
      181 aatatttaat aagatatacg taggcctatt gctagggctt gcgttacctg tccttacctt
      241 ctatgtgtat tttctgatca ggcaccatca ggagatttca tttgaatttt tcattagcat
      301 gttacataaa taccgtttgc tgtttaaagt ggtaagcttg tgtgtgctag tcaatttacc
      361 tgtattctat ggatttatcc agctaaagtt ctggcaaaca tcgaaaggta tcgtaatggc
      421 ttgcttcctt tttgcctttg ccgtagccgg ttatcaacta ttcacgtaag cccatgaagt
      481 attttctgat agcaggagaa gcttcgggcg atatgcatgg agcccggttg atggccgaag
      541 taaaagaata tgaccaaaaa gcggacttta tgtactttgg cggcgattta atgaaagagc
      601 aaggaggaac gctcttgaaa cattaccgcg aaatggcgta catgggaata ctacccgtgc
      661 ttactcacct gggggcaata aaacgtaatt taaccctgtg caaaaagcag ttgttgatgt
      721 tcaaccccga tgttgtgatt ttaatcgatt acccgggctt caacttgcga atcgccaaat
      781 ttgccaaaaa gaaaaaatac gctacagcct attacatatc tcccaaaatc tgggcatgga
      841 aaacccgcag gatcaagtcg ataaagcgtt acatcgacca gatgtatacc attttcccct
      901 ttgaaacttc cttctaccaa caattcaact atcaggtaaa ctatgttggt aatccggtgt
      961 acgacatcat tcaggaagag aaagaaaaaa aaattgattt tcagcaattc tgcaagaaca
     1021 ataatctctc gggcaaaccc attgtagccc ttcttgccgg tagccgcaaa catgaggtaa
     1081 aagagatgtt gcccataatg gaacaagtca gcgatcattt ttcggagtat gaatttgtta
     1141 ttgccggagt agacgcgctt ggctccaatt tttataaaga aacattaatc aaaaacctta
     1201 aagttgttta taatcaaacc tatgcattat tacaaaacag caaagcagcc ctggtaactt
     1261 cgggcacagc tacgctggaa accggcttgc tttatgtgcc acaagtagta tgttaccgca
     1321 tgggctttgg gtggttcctc gaaagaatta aagacaaagt gttgaaaacg aagtactttt
     1381 cgctaataaa tattatcgcg gggaaagagg ttgttaaaga actattccag agccaggtaa
     1441 acgtacagaa ccttagcaca gaacttgaca agctgcttca caacacagaa tatcaacaga
     1501 atatgctaaa caactatcga agtattgaaa acaccataaa aaccaacggt gctgcaaaag
     1561 cggcggccca actcattgtt gaatcgataa aaacacaaaa gaaagatgat acctctgctt
     1621 aaaaaagaac ttaatacatt tttcggatcg atcaccggat atttggtcat cgctatcttt
     1681 ttgctggcaa caagcctttt cttatgggta attcccggca atttcaacat catcgacgga
     1741 caacaagcct cgttatcagg ttttttcgag ctggcaccat ggctttatct tttcctaatc
     1801 ccagcgatca ccatgcgaat gtttgccgag gagaaaaaaa gcggcactat agaaatactt
     1861 ctcacacgtc ccataagtac atggggcatt gtgctttcaa aattcctggc atcgttactc
     1921 cttgtctgca tttcgctaat ccctaccctg ctctacttct ggtcggttgc gcgccttggt
     1981 aacccggtcg gaaatattga taccggggca acctggggtt cctttatcgg attattattt
     2041 ttagctgctg tatatatttc aatcggactt tttgcctcat cggcgacaag taaccaaata
     2101 atttcgtttc tggtttcctt gctcatttcg tacctctttt accttggctt tggatttatc
     2161 gtttatacct cagtttccga agcgtttaaa tcatttctta tcgaagccgg aattaatgaa
     2221 cattatatat cgataagccg cggagttatc gattcgacag acctgttcta ttttatactc
     2281 accagcctgc tgttcctttt tctttctggc ctgatgataa ggaaacagcg tatttcggtc
     2341 aaaaagatct tacccggatt ggggatttta gggttaacaa tagtcctcgt ttccatcccg
     2401 cttttcagaa ttgacctgac tcaggaaaaa agatactcgc tttcgccgca aaccaaagaa
     2461 atcattaaaa atcagaaata caacatcaac attgaactgt atcttgccgg agacctgcct
     2521 gccggaatga gaaattttca acaagaaatc attgataaaa tcgaagactt gaat
//
LOCUS       DAOPUW010000261         2570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_42238_length_2570_cov_3.672763, whole genome shotgun sequence.
ACCESSION   DAOPUW010000261 DAOPUW010000000
VERSION     DAOPUW010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42238_length_2570_cov_3.672763"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..1799)
                     /locus_tag="PKJ43_05300"
     CDS             complement(273..1799)
                     /locus_tag="PKJ43_05300"
                     /inference="COORDINATES: protein motif:HMM:NF010184.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HNZ72016.1"
                     /translation="MKDLQQVGFDNDKYLKEQTEAILKRVSRFNDKLYLEFGGKVLYD
                     YHAARVLPGFDPNVKMRLLQLLKDKIDVILCIYAGDIERNKIRADFGITYDVDALKTI
                     DDFREWGINVTAVVITRYQNQPAAAAFKNKLELRGMKVYLHYPTQGYPTDVDLIVSDQ
                     GYGANEYIQTTKPIVIVTGPGPGSGKLATCLCNLYHEYRNGIKAGYAKFETFPIWDLP
                     LTHKVNIAYEAATADLRDENLIDVHHLNAYNVEVVNYNRDIEAFHLLKRILEKITGGE
                     SMYQSPTDMGVNRASAGIVNNDIIEEAAHQEIVRRYFRCAVEYAMGMTEKDSLDRVKE
                     VMQKVGAKPEDRKVVLPAREAAKQAENCGKGNEGVYCGSSIQLHDGTIIVGKNSTLLH
                     SSSSLILNATKHLAGLPDNLFLLPQSIIDSVTHLKKDLLGGKMVSLDVEETLIVLSIS
                     ALSNPAAQMALENLKELRGCEVHTTHIPTPGDEAGFRKLGVNLTCDPEFSSKRLFVNE
                     "
     gene            complement(1973..>2570)
                     /locus_tag="PKJ43_05305"
     CDS             complement(1973..>2570)
                     /locus_tag="PKJ43_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNZ72017.1"
                     /translation="QKTIDLINTLDLFICAIFLGDFFYFLYAAKGKEARVEYLKHHWI
                     DFVASIPFMSFLRAFRLVRVVRIVRLLRGVKGIIHILRLLGTNKIQNIMISYFLILVL
                     VLFYCSLAFYSFELGVNPNVHCYFDAFWWAFVSLTTIGYGDIFPVTVEGRVAAMVLAL
                     AGIGLFSLITAQLATYFIKVQKQEQCDKKNDNGKHVEN"
BASE COUNT          784 a          565 c          480 g          741 t
ORIGIN      
        1 tctaaatcag tccgaaccta ttcatgtata atgccgatga atagaaatgt tcagtgcaat
       61 cgagtgggcg aagattggac tgttatattt cttcaatcgg tattaacctg agttcgacgt
      121 tattattgtc tttcagatag ccatgaaata catgacaaaa tctttataca atgcagaaac
      181 aattgcgttc acagccaaat taaattttaa agaataaggc aaactagcac agtgtaattc
      241 ctaacacact atatgcctgc tatttagtaa acttattcat taacaaataa cctcttgctg
      301 gaaaactcag gatcacaggt gaggtttaca ccaagttttc tgaaacctgc ttcatcaccc
      361 ggtgttggaa tatgggttgt atgaacttcg caaccacgca attctttcag gttctccaat
      421 gccatttgcg ctgcagggtt tgataaggca ctaatcgaca atacaataag tgtctcttca
      481 acatcaaggc tgaccatttt cccgcccagc aaatcttttt tcaagtgggt tactgagtcg
      541 ataatgcttt gaggcagcaa aaacagatta tcgggcaaac cggcaagatg ctttgttgcg
      601 ttgagtatca gactagaaga agaatgaagc aatgttgaat tttttccaac aataatggtt
      661 ccatcgtgca attgaataga agatccacaa taaacccctt cattaccctt accacaattt
      721 tcggcttgct ttgcagcttc gcgggcaggc aaaacaacct tccggtcttc gggtttggca
      781 cccactttct gcattacttc ttttacccga tcgagcgaat ctttttcggt cattcccatg
      841 gcgtattcaa cggcacacct aaagtaccgg cgcacaattt cctgatgcgc agcttcttca
      901 ataatgtcgt tattcacaat acctgcactg gcacggttta cccccatgtc ggtgggcgat
      961 tggtacatcg attcgccgcc tgtaattttc tcaagaatac gtttcagcaa atgaaaagct
     1021 tcaatgtctc ggttataatt caccacctca acgttatagg cattcaaatg atgaacatcg
     1081 atgaggtttt cgtcacgaag gtcggctgta gctgcttcgt aagcaatatt tactttatgc
     1141 gtaagaggca agtcccaaat aggaaatgtc tcgaactttg catagcctgc ttttattccg
     1201 ttgcggtact cgtggtataa gttgcaaaga caagtggcaa gttttccgct tccaggcccg
     1261 ggccccgtta caattacaat tggttttgtt gtctgaatgt attcattggc cccatacccc
     1321 tgatcgctga caataagatc aacatcggtt gggtagccct gtgttggata gtgtaaatac
     1381 actttcatgc cgcgaagctc aagtttattc ttaaatgcgg cagcagcagg ttggttttga
     1441 tatcgggtga taacaactgc agttacgttg atgccccatt ccctgaaatc gtcgatggtt
     1501 ttcagggcat caacatcgta agtgatgcca aaatcggccc ttattttatt gcgctcaata
     1561 tcgccggcat aaatgcaaag aataacatct atcttatctt taagaagttg caacaaacgc
     1621 attttcacat tcgggtcgaa acctggcaat acccttgcgg catgataatc gtaaagaact
     1681 ttccctccaa attcgagata caatttatcg ttaaaacggc taacccgctt gagaattgct
     1741 tcggtctgct ccttaagata cttgtcgtta tcgaacccaa cttgctgtaa atctttcatt
     1801 ttatttcccc ctaaaactga ctgaaaagga taaaattaaa gagttcaata ttagctattt
     1861 ttttaaggtc actaaaccaa aaaacacgtt tttttacatc atttgctgtt tctgccctga
     1921 caaaaagggt atttacgtag aacacgtata agcacatgat tatataacat acttagtttt
     1981 cgacatgttt cccgttatca ttctttttgt cacactgctc ttgtttctga actttaataa
     2041 aatatgtagc caactgagct gtaatgagtg aaaacaaccc gataccagcc aacgctagca
     2101 ccatggcagc taccctgcct tcaacagtaa cggggaaaat atcgccataa ccgatggttg
     2161 taagcgaaac aaaagcccac cagaaggcgt cgaagtaaca atgcacattg gggttcaccc
     2221 ctaactcaaa ggaataaaat gcaagagaac aatagaatag tacaagaacc aatattaaaa
     2281 aataggaaat cataatattc tgtatcttgt ttgttcctaa cagtctaaga atgtgaatta
     2341 tcccttttac cccgcgaagt aagcgcacaa tacgaacaac ccgcaccagc ctgaacgcgc
     2401 gcaaaaaact cataaaggga attgaagcta caaaatcgat ccagtgatgt ttcaaatatt
     2461 caacgcgggc ttctttacct tttgcagcat agagaaaata aaagaaatcg cccagaaaaa
     2521 ttgcacaaat aaaaaggtct aaggtattaa tcaagtcaat agttttctga
//
LOCUS       DAOPUW010000262         2548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_42777_length_2548_cov_3.890493, whole genome shotgun sequence.
ACCESSION   DAOPUW010000262 DAOPUW010000000
VERSION     DAOPUW010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42777_length_2548_cov_3.890493"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PKJ43_05310"
     CDS             complement(<1..245)
                     /locus_tag="PKJ43_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72018.1"
                     /translation="MLKYLFFSVLLIATAFAGFYFGSNRVRDGLVANYPTIKTLVNRS
                     FVEVDTSLTFPITAEETPALFALSSVSKTDSPTLTVRV"
     gene            complement(246..587)
                     /locus_tag="PKJ43_05315"
     CDS             complement(246..587)
                     /locus_tag="PKJ43_05315"
                     /inference="COORDINATES: protein motif:HMM:NF018002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72019.1"
                     /translation="MKKVTDGEQNTNTHWITWEVFHQEKKGKQAVHVGVVHAPSVDMA
                     VLFAKEAFARRGKTTNVWVVKTSDIYTVASEDEDIFESTPEKIHREPASYKVRDRIEA
                     YRTNYSSTLKQ"
     gene            complement(680..1264)
                     /locus_tag="PKJ43_05320"
     CDS             complement(680..1264)
                     /locus_tag="PKJ43_05320"
                     /inference="COORDINATES: protein motif:HMM:NF020295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNZ72020.1"
                     /translation="MEKKETMTLEIYQEATPNPESLKFVTNLHLLPNYYAEFKSADAC
                     GESELAAKLFTIPFVQSVFISNNFLTITKKPDYDWYEITPELKEVIKQFVYSDRPIVS
                     NALLEKGEAPKAQASDDPEAKIKELLDKYVKPAVEGDGGYIEFRSFTDGVVRLSLQGS
                     CSGCPSASMTLKGGIEGLLKRMVPEVTEVIAVEE"
     gene            complement(1299..>2548)
                     /locus_tag="PKJ43_05325"
     CDS             complement(1299..>2548)
                     /locus_tag="PKJ43_05325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ72021.1"
                     /translation="FSKNWFSDSALGFGHYLEKPHSISVSNMLANIGFGPLELVGTGQ
                     WYCGDSVVGGVQQCPDGSYSKQKVNQRIYIKNNFGFFDYKDTLIGTMGFHAALGHQHL
                     HVDNITENSLRIGGPETDPLQWPMLARGEKVSFSIYDHLRCDNTFEACEYNNKIYTSS
                     DFHNAGLGATYGATGFSIQGISVGYADLYEYNLDGQSIPLDSTLCNGDYYIFANFDPL
                     QRITDLNRDNNSVYGKITLTKQRSNCCETAFQIDTIDWPNRLFRFIDRSKAIPGEWHW
                     TFGDGDSSTAQFPLHQFKTNSNFAVHLKTKTNQGCEADMWQTLSLPQGLGINNRAVLS
                     SVAVYPNPSNDIFHIALEHEDNSLVDIAVVDIQGKAIAHYETGKNISDKKYSFDLRIE
                     DSGVYILIIKTNDAVKQIKLVKE"
BASE COUNT          720 a          559 c          503 g          766 t
ORIGIN      
        1 actcttaccg ttaaagttgg gctatctgtt tttgaaacgg acgacaaagc gaacaatgcg
       61 ggagtttcct ctgctgtaat tggaaaagta agcgatgtgt ctacttcaac aaaacttcgg
      121 ttaactagag ttttaatggt tggataattg gccactaaac catcgcgaac cctgttggag
      181 ccgaagtaaa atcctgcaaa tgctgtagca ataagcaaaa cagagaaaaa taaatactta
      241 agcatctatt gttttaaggt tgaactataa tttgttctgt aggcttcaat tctatcgcgc
      301 actttatatg atgcaggttc gcgatgaatt ttctctggag tggattcaaa tatgtcttca
      361 tcttcgctag caacggtata aatgtcggag gttttcacga cccaaacatt cgttgttttc
      421 cctcgtctgg caaatgcttc cttagcgaaa agcacagcca tatctacaga aggagcatga
      481 actacaccaa catgtacggc ttgttttcct ttcttttctt gatgaaacac ttcccatgta
      541 atccaatgtg tatttgtgtt ttgttctccg tccgttactt ttttcaagtc taaacggttc
      601 acgcgcgggt ctaaagatgt tggttgttgg ctcatactat tatcgctata gaaatattca
      661 aattggattc aaaaaacact tattcttcaa cggcaatcac ctccgttact tccggcacca
      721 ttcgcttcaa taggccttca atgcctcctt ttagcgtcat ggatgcagaa ggacaaccac
      781 tacaagaacc ttgcaatgaa agacgcacga caccatctgt aaagcttcgg aattcgatat
      841 agccaccatc gccctcaaca gcaggtttca catacttatc caacagttct ttaattttgg
      901 cttccggatc atcgctggct tgtgccttag gggcttcgcc tttttccaac aaagcgttac
      961 ttacaatcgg tcggtcggaa tacacaaact gcttaatcac ctctttcaac tccggtgtaa
     1021 tttcatacca gtcgtagtcg ggttttttgg tgatcgttaa aaagttgttc gaaataaaaa
     1081 ccgattgtac aaaaggtatc gtaaacaatt tagctgctaa ttctgattcc ccgcaagcgt
     1141 ctgctgattt gaactcggca taataattcg gcagtaaatg taagttcgtt acaaatttca
     1201 aactctcagg gtttggcgta gcttcctgat aaatttcaag tgtcattgtt tctttctttt
     1261 ccataaattt tttcaaggtt gataggatcg acatatactt attctttcac taatttgatt
     1321 tgtttcacgg catcgttcgt cttgataatc aagatataaa cgcctgaatc ttcaatgcgc
     1381 aggtcaaaac tatatttttt gtctgatata tttttgcctg tttcgtaatg cgcaatggct
     1441 ttgccctgaa tatcaacaac agcaatgtca acaagcgaat tatcttcatg ctccaaagca
     1501 atatgaaaga tatcgttgct tggattcggg tataccgcaa cagaagaaag aaccgctcga
     1561 ttgtttatac ctaatccctg cggcaacgaa agcgtttgcc acatatcggc ctcgcaacct
     1621 tggttagttt tcgttttaag gtgcaccgca aaattgctgt ttgttttgaa ctgatgtaac
     1681 ggaaattgag ctgtgctaga atcgccatcg ccaaaggtcc agtgccattc tcccggaatt
     1741 gccttactac gatcgataaa gcgaaacagg cggttcggcc aatcgatagt atcgatttga
     1801 aaagccgttt cacagcaatt ggagcgctgt ttagtcaaag taatctttcc atacaccgaa
     1861 ttattatctc gatttaaatc ggtaatgcgc tgcagtgggt cgaagttggc aaaaatgtaa
     1921 taatcgccat tgcatagtgt tgagtcgagc ggaatgcttt gcccatcgag gttatactca
     1981 tacaaatcgg catagcctac agaaatacct tgtatagaaa aaccggtagc gccataagtg
     2041 gctcccagtc cggcattatg aaaatcggac gaagtgtaaa ttttgttgtt gtactcacag
     2101 gcttcgaatg tattgtcgca acgcaaatga tcgtaaatgg agaacgaaac tttttctccg
     2161 cgggcaagca taggccattg caaaggatcg gtttccggcc caccaatacg cagactgttt
     2221 tcggtaatat tatcgacatg taaatgctga tgccctaaag cggcatggaa gcccatggtg
     2281 ccaattagcg tgtctttata atcaaagaac ccaaaattgt ttttgatata gatgcgctga
     2341 ttcacttttt gtttagaata gctaccatcc ggacattgtt gcacgccacc caccacacta
     2401 tcaccacaat accattgccc cgttccaacg agttcaagcg gcccaaaacc aatgttagca
     2461 agcatgttgc tcacactaat gctgtggggc ttttctaaat aatgcccaaa gcctaacgct
     2521 gaatctgaga accaattttt ggaaaagc
//
LOCUS       DAOPUW010000263         2546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_42822_length_2546_cov_3.066238, whole genome shotgun sequence.
ACCESSION   DAOPUW010000263 DAOPUW010000000
VERSION     DAOPUW010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42822_length_2546_cov_3.066238"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PKJ43_05330"
     CDS             complement(<1..216)
                     /locus_tag="PKJ43_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72022.1"
                     /translation="MKQKIFTLVLMLAMVFVASSAWAQNDANVTPGGTYSYSLSGITS
                     VYAATANVTYGGTNVAISNYRLDGTVIS"
     gene            complement(992..1903)
                     /locus_tag="PKJ43_05335"
     CDS             complement(992..1903)
                     /locus_tag="PKJ43_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ72023.1"
                     /translation="MYDIIGDIHGHYKLLVAMLRKLGYEKSGEGYACPGRKLIFTGDY
                     INRGPEIRKTVALVRQMVESGNAMAILGNHELNAILYFSLDKDSKLFRKHAAHLRLQL
                     LSTIDDFGSNTEELKDHIKWFRKLPFYLDLGSIRVVHGGWNDSHVETVNRFMDGNDKI
                     KKSFLRDYLIRDDLYVAVNELIRGVELQLPKDLIVRDSKGISHRTFRVCWWEPALGKT
                     FADASFGTRFILPPYTIPREVVPEIATYTDDLPPVFFGHYCLEKQDLIIKSNLCCVDR
                     CVTRSQTLTVYRWNGETNLKEENLVHL"
     gene            complement(1947..>2546)
                     /locus_tag="PKJ43_05340"
     CDS             complement(1947..>2546)
                     /locus_tag="PKJ43_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72024.1"
                     /translation="SVTDKIDALEKVYRKLEVLAQYKPDFQYHTYANDRELKHDKTLF
                     HLHSSWVTMFWQRSNPEGEKLMRPCEIPSMTGKTAYAYSGTYNCIFVVPQKAKNRNEA
                     IRLMKFICSLDIAEKWENYSKCPTGLKSRISVTEFGSDNFSLFSQHVTRKFDNRLQDR
                     TLSGIFFGNSQRSVNFNALNVLHGEISASQAVGNIRRQL"
BASE COUNT          754 a          553 c          493 g          746 t
ORIGIN      
        1 actaatcaca gtaccatcga ggcgataatt actaatagct acattcgtac caccatacgt
       61 tacattagca gttgctgcat aaacggaggt aataccagat aaagaatagc tatacgttcc
      121 cccaggagtc acatttgcat cattttgtgc ccaagcactg cttgcaacaa ataccatggc
      181 aagcattaat acgagtgtaa aaattttttg tttcataact ttaaaatttt ggtttaacaa
      241 tatttattat ttcttgtttt attatcaacg cactgcgcca ggcttgcacc cgcgcggctt
      301 attgttttcg tttatttatc gcccggtaat acaccaaaac tggcacccct accctagcta
      361 catgtataca atttaaaaaa tattttgtga aaaacgatgg gattatatta aaattttcgg
      421 gtatgtaatt agccacagca aacttgtttt gcatgcaccc taaattacta tttattctgg
      481 cattatagct cacccgcata actccccttg tattttcctc tttttccctc actcgtatac
      541 acaacatcag gcaaaagcaa aagctttcac ctaaattaat tcattaattt acaattgtat
      601 ggatgaaaaa gatataacaa aatcattagc acaccggtag ccggctcaat gcccatgtgt
      661 agttgttttc cccataaaat tgttttcctc tcttaattct gattactaaa tataaacatt
      721 caatacttca gatggttcaa aaataatgat tatcagttaa taaaaaacag gcaattgtca
      781 tttttttcat tttccaacgt ttttttatta ttcaattaat tcttaaaacg gagaaaggag
      841 gagggaaacc ggaaaaggga aaaaggagaa tgaagaaaat tgtcaattat ccgttgccca
      901 gggctgaatc ttgcaggccg gtagccggag gatgagacgc gatgcatcgc gtctgtacaa
      961 ttgtcaattg tcaactgtca attgtcaatt gctacaagtg tacaaggttt tcttctttca
     1021 agttggtttc gccattccat cggtacacgg taagagtttg cgaacgggtc acacaacggt
     1081 cgacacagca aagattgctt tttatgataa gatcctgttt ctcgaggcaa taatgtccaa
     1141 aaaaaactgg cggcaggtca tcggtatagg tagcaatttc gggaaccact tcgcggggaa
     1201 tggtataggg cggcaaaata aaacgcgtac caaacgacgc atcagcaaaa gttttaccca
     1261 aggccggctc ccaccagcat accctgaacg tacggtgcga aatacctttc gagtcgcgca
     1321 caatcaagtc tttgggcaac tgcagttcaa ccccacgtat caattcatta acagcaacat
     1381 acaagtcgtc acggattaaa tagtcgcgca agaaactctt ttttatctta tcgttgccat
     1441 ccataaagcg gttgactgtt tctacatgag aatcgttcca cccgccatgc accacacgaa
     1501 tgcttcccag atcgaggtaa aaaggaagtt ttctgaacca ttttatgtga tctttcagtt
     1561 cctcggtatt gctgccaaaa tcgtcgatag tagaaagcaa ctgaagcctc aggtgtgctg
     1621 catgcttacg gaatagcttc gagtctttat cgagtgaaaa atacaaaatg gcattcaact
     1681 cgtgatttcc caggatagcc atggcattcc cgctttcaac catttgacgg acaagggcta
     1741 ccgttttcct gatttcggga ccacgattaa tataatcgcc ggtaaaaatg agtttacgcc
     1801 cggggcaggc atagccttcg cccgacttct cgtagcctaa ttttctgagc atcgcaacca
     1861 gaagtttata atgcccgtgt atatctccaa taatgtcgta catatcgttt ttaattggga
     1921 aaagtaatca gcactgcaac tacaatctaa agctggcgcc gtatattgcc aacggcctgg
     1981 cttgccgata tttcaccatg caatacattt aaagcattaa agttgacgct ccgttgagaa
     2041 tttccaaaga aaatcccgga taatgttcta tcctgcaatc gattgtcaaa cttacgagtc
     2101 acatgttgcg aaaaaagact gaaattgtcg gaaccaaact ctgtaaccga aatccggctt
     2161 ttaaggcctg tagggcattt cgaatagttt tcccatttct cggctatgtc gagcgaacag
     2221 ataaatttca taagacgaat ggcttcattg cggttctttg ctttttgggg cacaacaaaa
     2281 atgcagttgt acgtgccact ataagcataa gcagtttttc cggtcatgga tgggatttcg
     2341 catggccgca tcagtttttc tccctcagga tttgagcgtt gccaaaacat ggtaacccac
     2401 gaagagtgaa ggtgaaacaa tgttttgtcg tgcttcagtt cacggtcgtt tgcataagta
     2461 tggtattgaa aatcgggttt atattgagcg agtacttcca attttctgta aaccttttcg
     2521 agggcatcga tcttatcggt cactga
//
LOCUS       DAOPUW010000264         2541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_42935_length_2541_cov_3.838294, whole genome shotgun sequence.
ACCESSION   DAOPUW010000264 DAOPUW010000000
VERSION     DAOPUW010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42935_length_2541_cov_3.838294"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="PKJ43_05345"
     CDS             complement(<1..813)
                     /locus_tag="PKJ43_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HNZ72025.1"
                     /translation="MAIIKPFKGLRPPKNIAQKVASRPYDVLNSDEARVEAAGNQYSL
                     LRIIKPEIEFPAGTDEHSPEVYEKAVEMFKLFQQEKWLVQDPKDCLYIYAQTMNGRTQ
                     YGLVACASVDDYLNNVIKKHELTRADKEEDRMKHVRNTNANVEPVFFAYPAVEKIDAI
                     VSEIVTSKPAEYDFVAEDGFGHHFWVIDNEETIQQLVELFAKIPSTYVADGHHRTAAA
                     ALVGAEKRKLNPNYNGSEEFNYFLAVHFPDNQLQIIDYNRVVKDLNGLDKTGL"
     gene            complement(878..1948)
                     /gene="serC"
                     /locus_tag="PKJ43_05350"
     CDS             complement(878..1948)
                     /gene="serC"
                     /locus_tag="PKJ43_05350"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNZ72026.1"
                     /translation="MKKHNFSAGPSILPEFTKEETAKAIIDFAGTGLSVMEVSHRGAE
                     FQAVVDETIQLIKEILSVPEGYSVLLLQGGASLQFYMAPLNLLVKKAAYLNTGVWASK
                     AMKEAKLVGEVIEVASSKDKNHTYVPKGYEVPADVDYFHITTNNTIYGTEMFSDPEVC
                     VPLVADMSSDIFSRPVDISKYDLIYAGAQKNLAPAGLTLVIVKDAALGKTGRTLPTML
                     NYQTHIDNGTMFNTPPCVAIYAALQTLKWIKNLGGLEVIQKMNVAKAKVIYDELDRNK
                     CFKAVVPNKEDRLLMNICFVMREGYEAVEADFLALAKQRNLIGLKGHRSVGGFRASTY
                     NALPIESAQALVDALKEFEANH"
BASE COUNT          681 a          485 c          529 g          846 t
ORIGIN      
        1 taatcctgtt ttgtcaaggc cgttaaggtc ttttaccacg cggttataat cgatgatttg
       61 aagttgattg tcggggaaat gaacggcaag aaagtaatta aactcctcac ttccattgta
      121 atttggattt aacttccttt tttctgcacc aaccaaagcg gccgctgcgg tacgatggtg
      181 tccatcggct acatatgtcg aaggtatttt ggcaaataat tcgacaagct gttggatggt
      241 ttcttcgtta tcgatcaccc aaaaatgatg tccaaagcca tcttcagcaa caaaatcata
      301 ttcagctggt ttcgatgtaa caatttcact tacaatggca tcaatttttt caacagccgg
      361 gtaggcaaaa aaaacaggtt caacatttgc gttggtattc ctcacatgtt tcatgcggtc
      421 ttcttctttg tcagcccggg tgagttcatg ttttttaatg acattattaa ggtaatcatc
      481 aaccgaagca caggcaacca agccgtattg agtgcgacca ttcatggttt gagcatagat
      541 gtaaaggcag tcttttgggt cttgaacaag ccatttctcc tgttgaaaaa gtttaaacat
      601 ttcaacagct ttttcgtaca cttcgggcga atgctcatcg gtgccggcag gaaattctat
      661 ttcaggtttg attatgcgca acagcgagta ttggttgcca gcagcttcaa cccgtgcttc
      721 gtctgaatta agtacatcgt agggccgtga agcaactttt tgtgcaatat ttttcggggg
      781 acgtaatcct ttgaatggtt ttattattgc cattattgtg tggtattttt tgtgaaaaac
      841 gagatgcagg gtgttacctc tgcatctcgt ttatttttta gtgattagct tcgaattctt
      901 tcaatgcatc aaccaatgcc tgagcacttt caattggtag ggcattatat gttgatgccc
      961 tgaaaccacc aaccgatcga tggcctttta agccaatcag gttgcgttgt tttgcaagag
     1021 caaggaaatc tgcttcaaca gcttcgtaac cttcgcgcat tacaaaacag atgttcatta
     1081 acaagcggtc ttctttattc ggaacaacgg ctttaaagca cttgttacgg tcgagttcgt
     1141 cgtagattac cttggcttta gcgacattca ttttctggat aacttctaaa cctccaaggt
     1201 tttttatcca tttaagtgtt tgaagagcag catatattgc cacacacgga ggtgtgttaa
     1261 acatagtgcc gttatcgatg tgggtttggt aatttagcat tgtaggcagt gtacggccag
     1321 ttttaccaag cgctgcatct ttcacaatta ctaaggttaa cccagcagga gcaaggtttt
     1381 tctgtgcacc ggcataaata aggtcatatt ttgaaatgtc aactgggcgg ctgaaaatgt
     1441 cagacgacat atcggcaact aatggaacgc aaacctctgg gtcactgaac atctctgttc
     1501 cataaatagt gttgttggtt gtgatgtgga aataatcaac atcggccgga acttcgtacc
     1561 ctttaggtac ataagtgtgg tttttatctt ttgatgaagc gacttcgata acttcgccaa
     1621 ccaacttagc ttctttcatt gctttgcttg cccaaactcc ggtattgaga taagctgctt
     1681 ttttaaccaa caggttgaga ggagccatgt aaaattgtaa gctggcacca ccttgcaaaa
     1741 ggagaactga atatccttca ggaacagaaa ggatttcttt aattagttgg atggtttcat
     1801 caacaactgc ttggaattca gcaccacgat gcgaaacttc cattaccgat aatcctgttc
     1861 ctgcaaaatc tataatagct tttgctgttt cttcttttgt aaattcaggc aaaatcgatg
     1921 gccctgcaga aaaattgtgt tttttcataa acttatttat tgatttttat tttgttagat
     1981 gtaaaaactc ttttctcgat gaacgacaag ctttgtgtct ttcttcgtat ttgtaagcta
     2041 tttaaccgat gcaagatcgg catgaacagt aacatgatgt aaaccacccc atctgaacgg
     2101 ggttccattc gttgattagc aatgtgtggt ttctcttttt cataatacct atgaatgaag
     2161 tttttgcagt cttttctggt gttgggtcaa cgttcttttt tgttaactct gatcactaga
     2221 agcgattttc ggcttgcgac tgtttgaagc gataaagata aaataaatct tctttgattt
     2281 aaaattgtcg aaataattgt gctttttttt gaaaaaatgc aagtgattta cgtgatatgc
     2341 ttaatgtttt attaaaaaaa gaggctatgc gtaaaaacat agcctctttt ttaaattaaa
     2401 atggtaaatc gttatacgcc tcggcgtcat actggttgtt gggtggggct ggtatctcgt
     2461 cgggaatatc ggctctcgaa aaattaggct gttcctgagt cgattgcgct gacgaaattt
     2521 tccaggcgtt gatgttgcta t
//
LOCUS       DAOPUW010000265         2522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_43395_length_2522_cov_2.985002, whole genome shotgun sequence.
ACCESSION   DAOPUW010000265 DAOPUW010000000
VERSION     DAOPUW010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43395_length_2522_cov_2.985002"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..553)
                     /locus_tag="PKJ43_05355"
     CDS             complement(260..553)
                     /locus_tag="PKJ43_05355"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNZ72027.1"
                     /translation="MKRIVWTPEAVKTYHSLVQYLALEWGNLSKTKFLNEVELVVDLI
                     SRNPRMFKRSTRYENIRIGYLSKQCSLIYRIKPDEIELLLFWGNRQDSKKLKY"
     gene            complement(550..786)
                     /locus_tag="PKJ43_05360"
     CDS             complement(550..786)
                     /locus_tag="PKJ43_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72028.1"
                     /translation="MEYSKLKEELHQIIDQVNDEAALYQVRSILEAQTGHDWADDMSD
                     ELREALERSIAQADAGNVISHEEVMKMLREKYGQ"
     gene            995..1951
                     /locus_tag="PKJ43_05365"
     CDS             995..1951
                     /locus_tag="PKJ43_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNZ72029.1"
                     /translation="MAAKVSIGVDIGGTNTEFGIVDGDGKVIERAMIPTPKKDNINQY
                     VDDLSAGIKRLISKYSDFDYQGIGVGAPDGNFYKGTIEHASNLPFQGVVPFVSLLKER
                     FPEMKKIALTNDANAAALGEMLYGGAKGLKNFIMITIGTGLGSGFVVNGELMYGSDGF
                     AGELGHVTVTAEGRECGCGQFGHLETYCSATGMVRTAFELLSHHNATESLLANKSFNQ
                     TTSKDIFDAAKKGDEIALTVFEITGDILGQALADAVHISSPEAIFLFGGPVAAGEMLF
                     KPIRESMESHLMPMFRGKVKLLPSRLRQGEAAILGASALVCK"
     gene            complement(2012..>2522)
                     /locus_tag="PKJ43_05370"
     CDS             complement(2012..>2522)
                     /locus_tag="PKJ43_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNZ72030.1"
                     /translation="KIISTVGLEDSKNFDVSLRLTPTDAPINVDNINYEFGKFDLLPG
                     SIQSLDSLVTLLELNPTIVIEIMSHTDFVGSAQFNSELSQKRAQSVVNHLISKGINPA
                     RLVAKGYGETWPKKVTKKMAKQYDFFSAGDELSEEYINKLQTDEQKEIAKSLDRRTEF
                     RVLRNDFIE"
BASE COUNT          693 a          516 c          552 g          761 t
ORIGIN      
        1 aatgccctct tcgatggcca tccgcaactc atcgctcatg taatcggccc agtcgtgacc
       61 ggtttgtgct tccaggatgg aacgcacctg gtacaacgca gcttcatcgt ttacctgatc
      121 gattaactgg tgcaattcgg ctttcaaatc ggaatattcc atggcaactt tttttctcaa
      181 aattaaggaa ataggatgag atttgaacta attgtggaca gaaagaaaaa ccacagtagg
      241 caaagtagaa cacataggtt caatatttca gtttcttcga atcttgtcga ttaccccaaa
      301 aaagcagcaa ttcaatttca tcaggcttaa tcctgtaaat caatgaacat tgttttgaca
      361 aatatcctat cctgatattc tcataccggg ttgaacgttt aaacatccga ggatttctgg
      421 agattaaatc aacaactaat tcaacttcat ttaaaaattt agttttagag agattacccc
      481 attctaaagc taaatattga acaagtgaat gatatgtttt gactgcttca ggagtccaaa
      541 cgattctttt cattgtccat atttctctcg aagcatcttc attacctctt catggctaat
      601 cacgtttccg gcatcggctt gggcaatgct tctttcaagt gcttcacgca attcatcgct
      661 catgtcatca gcccagtcgt gaccggtttg tgcttccagg atggaacgca cctggtacaa
      721 cgccgcttcg tcattgacct gatcgattat ctggtgcaat tcctctttca gttttgagta
      781 ttccattgat aatattttcc tcaaaaataa gcatttatga acaaaacaac aacggaggca
      841 tcatcggctc agcctttgtc aagtcttcta agtcttgctg ccatcgttgc gccccggcaa
      901 ctcttccaat aattaaatac tacttacagc ataattgctt aaacccatga taaaattggt
      961 tagctttacc acttcatctt gaaataaaaa gaatatggca gcaaaggttt ctattggggt
     1021 cgatatagga ggtacaaata ctgaatttgg cattgttgac ggcgacggga aagtcatcga
     1081 aagggcaatg atccctactc ctaaaaaaga taatatcaat cagtatgtcg atgatttatc
     1141 ggcaggaata aagaggctta tttcaaaata cagtgatttt gattatcagg gtatcggagt
     1201 tggtgcacct gatggtaatt tctacaaagg gactattgag catgcttcaa acctgccgtt
     1261 tcagggagtg gttccttttg tttccttatt gaaagaacgg ttccctgaga tgaaaaagat
     1321 tgccctgacc aatgatgcaa atgctgcagc cctgggcgaa atgctttatg gaggagctaa
     1381 gggattgaaa aattttatca tgataaccat tggaacaggt cttggaagtg gttttgtggt
     1441 gaatggcgaa cttatgtatg gcagcgatgg ctttgcgggt gaacttggcc acgttaccgt
     1501 gactgccgaa ggcagggagt gtggctgtgg acaatttggc cacctcgaaa cctactgttc
     1561 tgcaacgggc atggttcgta ccgccttcga attgttgtcg catcacaacg ctacagaaag
     1621 cttgttggcc aacaaaagtt tcaaccaaac aacatccaaa gatattttcg atgctgcgaa
     1681 aaagggagat gaaattgccc ttaccgtttt tgaaattacc ggcgacattc ttggtcaggc
     1741 tcttgccgat gccgtgcata tttcaagtcc tgaagcgatt ttcctttttg gagggccggt
     1801 tgctgcaggt gaaatgcttt tcaaaccaat tcgtgaatcg atggaaagcc acctgatgcc
     1861 catgtttcgt ggtaaagtta agttattgcc gtcgagatta cgtcagggag aagcagcaat
     1921 tctgggtgcc agtgcgttgg tttgtaaata attgtaatat atatcgaata aaaaacctca
     1981 ccgttcgcga tgaggttttt tttgattcag cttattctat aaaatcgttt cgcaagaccc
     2041 tgaactcagt gcgccggtcg aggcttttgg caatttcctt ttgttcgtcg gtttgaagtt
     2101 tattaatgta ttcttctgaa agttcatcgc cggcactaaa aaaatcatat tgtttggcca
     2161 tcttctttgt aactttcttt ggccatgttt cgccatagcc tttggctact agtctggcgg
     2221 ggtttatgcc tttcgaaata agatggttga caacagattg agcacgtttt tgtgaaagct
     2281 ccgagttaaa ctgggcgctg cctacaaagt cggtgtgcga cataatttct ataacaattg
     2341 tcgggttcag ctcaagtaac gtgactaaac tgtcgagcga ctggatggag ccgggcaaaa
     2401 ggtcgaactt gccaaattcg taattaatat tgtcgacatt gataggggca tcggtagggg
     2461 tgaggcgaag gctaacatcg aaatttttac tgtcttcgag ccccacggtg gaaattatct
     2521 tt
//
LOCUS       DAOPUW010000266         2510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_43668_length_2510_cov_3.356415, whole genome shotgun sequence.
ACCESSION   DAOPUW010000266 DAOPUW010000000
VERSION     DAOPUW010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43668_length_2510_cov_3.356415"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1547)
                     /locus_tag="PKJ43_05375"
     CDS             complement(<1..1547)
                     /locus_tag="PKJ43_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNZ72031.1"
                     /translation="MDKKTFVEKIQKSYTFKGESILIGSAILGDDYETGCQIRLPLKT
                     LNRHGLIAGATGTGKTKSLQVIMENLSDAGVPVLAMDIKGDLSGIGAEGETNAVVEKR
                     TKLIGMEWKPKSYPIEFLSISEEPGVRLRATVSEYGPVLLSKILGLNDSQEGILSMVF
                     KYCDDRNLPLLDLKDLKSVLSYAQEEGKEEFKREYGFLSTSSTGIILRNIITLEQQGG
                     ERLFGEPSFDVNDLMVTNNRGQGMINILRLTDIQDKPDLFSSFILCLLAELFQTMPEL
                     GDPEKPQLVIFIDEAHLVFKNATQNLLDQLEMTIKLIRSKGVGIFFITQSPTDIPASV
                     LGQLGTKVQHALRAFTAKDRKDIKTASENYPYSDFYVVDEMITQLGTGEAFVSGLDEK
                     GRPTELAYTLMRPPYSRMDVLTEQEINSHIRSSSLAREYNVEIDRESAFEILQSRVES
                     KEEEVERKMEDARTAPKGRTRVEQSTLEKIIKSPVTNTIAREVTRGLLGVLGIKTTTR
                     SRSRSRF"
     gene            1718..2125
                     /locus_tag="PKJ43_05380"
     CDS             1718..2125
                     /locus_tag="PKJ43_05380"
                     /inference="COORDINATES: protein motif:HMM:NF025589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HNZ72032.1"
                     /translation="MAKKIAEIEGIGPVYAEKLAALEITTVEKLLEEGASKTGRKKIA
                     EASGIDESKILKWVNMADLFRIKGIGQQFSELLEAAGVDTVKELRNRNAENLHAKLME
                     IQEQKKLTKAVPALSQVADFIEQAKGLEPMVTY"
BASE COUNT          766 a          535 c          472 g          737 t
ORIGIN      
        1 ctgaaccggg aacgagacct tgaacgtgtt gtggttttta ttcccaaaac accgagtaac
       61 ccacgggtta cttcgcgtgc tatagtattg gttaccgggc ttttaattat cttttcaaga
      121 gtcgactgct ctacccttgt cctccccttt ggtgcagtgc gggcatcttc catcttacgc
      181 tcaacctctt cctcttttga ctctacccta ctctgcaaaa tttcgaaagc actttcgcga
      241 tcaatttcta cattatattc cctggctaac gaagaacttc tgatatggga attgatttct
      301 tgttcagtta acacgtccat tcgtgaataa ggcgggcgca tcaaggtgta agccagctcg
      361 gtaggcctcc ctttttcgtc gagccctgaa acaaaagctt cacctgtacc caactgagta
      421 atcatctcgt caacaacata aaaatcggaa tatggataat tttccgaagc tgttttaatg
      481 tcttttcggt ctttggcggt aaaagcgcgt aaggcatgct gaactttagt tcccaactgc
      541 ccgagcactg aagcgggtat gtcggtaggc gactgcgtga taaagaaaat gccaacgccc
      601 tttgagcgga tcagttttat cgtcatttct aattgatcga gcagattctg ggttgcattc
      661 ttaaatacca ggtgggcttc gtcgataaaa ataaccaatt gtggtttttc tgggtcaccc
      721 aactctggca tagtttgaaa caattcggcc aacaaacaca gaatgaacga actaaataaa
      781 tctggtttat cctgaatatc agttaaacgt agaatattaa tcattccttg tccccgattg
      841 tttgttacca tcaaatcgtt gacatcgaac gatggctctc caaacaagcg ctcgcctcct
      901 tgctgttcga gggtaattat attgcgaaga atgattccgg tagaagaggt agacaagaaa
      961 ccatattcac gtttaaattc ttcctttcct tcctcttggg cataactcag cacgcttttc
     1021 aagtctttca ggtcgagcaa gggcaaattg cggtcgtcgc aatacttgaa aaccatcgac
     1081 aaaattcctt cctgactgtc gttcagcccc aggattttag agagtagaac tggcccgtat
     1141 tccgacacgg tagcacgaag cctcacccct ggttcttcag atatcgacag aaattcaata
     1201 ggataacttt tgggcttcca ttccatcccg atcagttttg tccgtttttc aaccacagca
     1261 ttagtttcac cttcggctcc aatcccgcta aggtcacctt taatatccat ggccaacact
     1321 ggaactcctg catcggaaag attttccatg attacctgaa gcgatttagt tttcccggta
     1381 ccggtagccc cagcgattaa cccatgacgg tttaacgtct tgagtggcaa ccttatctga
     1441 caaccggttt cataatcatc gcccaagatg gcagagccaa ttagtatcga ttcaccttta
     1501 aatgtgtacg atttctgtat cttttcaaca aacgtcttct tgtccatttc gaataatagt
     1561 tataaattac acggaagtta gtaaaaattt caattcataa aaattcgatc caaaataagt
     1621 gattttatga atttattaaa aaacaattta acaataaagc aaaaaccatc tgaaatacag
     1681 actttaaaaa atatcttact attaaaagtt aataattatg gcaaaaaaaa ttgcagaaat
     1741 tgaagggatt ggacccgttt atgcagaaaa attagctgca ttggaaatca caacagtaga
     1801 aaaactgctt gaagaaggtg cgtcgaaaac aggcagaaag aaaattgcag aagcttcggg
     1861 aattgatgaa tccaaaattc taaaatgggt caatatggcc gacttattcc gcataaaggg
     1921 tattggccaa cagttttcag aacttcttga agccgctggt gtggatacgg ttaaagaatt
     1981 gcgaaacaga aatgctgaaa acctgcacgc caaattaatg gaaattcagg aacagaaaaa
     2041 actcaccaaa gcggttcctg cactaagcca ggttgccgat tttattgagc aggccaaagg
     2101 gcttgaacca atggttacgt attgaaccaa aaagtaattc agcttgtttg aataacagat
     2161 cactggttta acagaattat ttcgatttcg gactgagcgc tgacaaaaca caaatagtca
     2221 gttttgttcc aaatcgtttt cataattttt acagaaattt tttggtgaaa cactttatct
     2281 taaagaagac cttgtagtat tacttggtct tcttttgtta tatccctgat tgaatatttg
     2341 ttacaaatgc aaaaataaca acaatctagg atcacttcat aattggggtt aacactttat
     2401 aatccttatt tagcctcaac tcgaaggaag aaaaaattca acagcttttt ttgatagaat
     2461 ggccactaat gcacgaatat tataccagat gcaattctaa atcagtccga
//
LOCUS       DAOPUW010000267         2510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_43674_length_2510_cov_3.020774, whole genome shotgun sequence.
ACCESSION   DAOPUW010000267 DAOPUW010000000
VERSION     DAOPUW010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43674_length_2510_cov_3.020774"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PKJ43_05385"
     CDS             <1..323
                     /locus_tag="PKJ43_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HNZ72033.1"
                     /translation="LKWNKSLILLLKKMIEKAQQYLASVQNSDGSWGGDVNIEGTIEE
                     TSLAVTALKGSSFSSNADKGLAWLDQQYCTKGLKASPIGLYFASLWYDEKLYPLTMYL
                     EALV"
     gene            435..917
                     /locus_tag="PKJ43_05390"
     CDS             435..917
                     /locus_tag="PKJ43_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNZ72034.1"
                     /translation="MKTQTFYDFSIHTPKGEKVEMSHYQGKVVLVVNTATKCGLAPQF
                     DGLEQLHQKYKDRGVVVLGFPCDQFLNQEPETNETVVEACRINHGVTFPLFEKIDVNG
                     KNTHPLYQYLKAELPGFLGGKIKWNFTKFLIDKNGKPVKRYSPTTVPEKIEVDILKLL
                     "
     gene            918..1346
                     /locus_tag="PKJ43_05395"
     CDS             918..1346
                     /locus_tag="PKJ43_05395"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNZ72035.1"
                     /translation="MDNFEQLKLENQLCFPLYTASRLVVRSYTPLLNRLGITYPQYLV
                     LLLMWEHRTLTVSQITKQLYLDTSTITPLLKRLEKIGYIKRTRSSEDERVVMIGLTEA
                     GEKLRDEACSIPAQLADELNFSEEELVELYRLLYKYIHQS"
     gene            complement(1411..>2510)
                     /gene="speA"
                     /locus_tag="PKJ43_05400"
     CDS             complement(1411..>2510)
                     /gene="speA"
                     /locus_tag="PKJ43_05400"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HNZ72036.1"
                     /translation="FYAELCKLGFPIEFVDIGGGLGVDYDGTRSSNSESSVNYSVQEY
                     VNDSVSTFVDVSDKNGLHHPNIITESGRSLTAHHSVLVFEVLETATLPEWDDEKDTVH
                     PDDHELVQEMYKIWDNLNQTGMLESWHDAQQIREESLDRFALNLLDLKTRALIERLFW
                     SIAREVFDMAAEIKHIPFELKQLPKMLADKYFCNFSLFQSLPDSWAIDQIFPIMPIHR
                     LEEKPIKNATIQDITCDSDGKIDNFISTKNVSYYLPLHKLKSTEPYYIGVFLVGAYQE
                     ILGDLHNLFGDTNAVHVSVNEKGYHIDQIIDGETVAEVLDYVQYSAKKLVRTVETWVT
                     ASVKQGKITASEGKEFLSNYRSGLYGYTYLE"
BASE COUNT          724 a          510 c          556 g          720 t
ORIGIN      
        1 cattgaaatg gaataaatcg ttgattcttc tgttaaaaaa aatgattgaa aaggcacagc
       61 agtatcttgc ttctgttcag aatagcgatg gcagctgggg cggcgatgtc aacatcgaag
      121 gaacaatcga agaaacctca ttggcggtaa cagccctcaa aggttcgtcg ttcagttcaa
      181 atgctgataa gggattggct tggctcgacc agcaatattg cacaaaaggg ttaaaagctt
      241 cgccaatagg gctatacttt gcttcgttgt ggtacgacga aaagctctat ccgctcacca
      301 tgtaccttga ggcgctggta taaaatctga aaattattgt agtatttcaa ctttcctatt
      361 gtttgtaaaa aataattttc tacatttgta tcgcatacga tataattgta aacgatttaa
      421 aataatgaat agacatgaaa acacaaactt tttacgattt tagcattcac acacccaaag
      481 gggagaaagt agagatgagc cattatcagg gcaaagttgt tctggttgta aacactgcaa
      541 ccaaatgtgg tttggctcca caattcgatg ggcttgaaca gcttcatcag aaatataagg
      601 atcggggagt ggttgtgctg ggttttccct gtgatcagtt tttgaaccag gaaccggaaa
      661 caaacgaaac ggtggttgaa gcttgccgga tcaaccatgg ggtaacattt ccgctttttg
      721 aaaaaatcga tgtcaatgga aaaaataccc acccgttgta ccaatactta aaggctgagt
      781 tgcctggttt tttgggtgga aaaatcaaat ggaatttcac caaatttctg atcgataaaa
      841 acgggaaacc agtgaagcgt tattcaccca caacggttcc tgaaaaaatt gaagtggaca
      901 ttctgaaact tttgtaaatg gataattttg aacaactaaa gctcgaaaac caactttgtt
      961 ttccgctgta tacggcatca aggttggtgg tgcgaagtta tacccctttg ctgaaccgtt
     1021 tggggataac ctatccgcaa taccttgttt tgttgctgat gtgggagcac cgaacgctga
     1081 ccgttagcca aatcaccaaa caactgtatc tcgataccag tacaatcact ccgctgctaa
     1141 agcggttgga aaaaattggg tacataaaac gaactcgttc ctccgaagat gaacgggtag
     1201 tgatgatcgg attaaccgaa gcgggtgaaa agttacggga cgaagcgtgt tctattccag
     1261 cccagttagc cgatgaactg aatttttcgg aagaagagct tgttgaacta taccggttgc
     1321 tttacaaata cattcaccaa tcatagaaat ctaaaaaaga agcggagatg tttataatcc
     1381 ccgcttcatt aatgtaaata gtattcgatt ttactccaga taggtgtatc catacaaacc
     1441 tgagcgatag ttagaaagga attccttccc ttccgaagct gtaatttttc cttgcttcac
     1501 cgaggctgtt acccatgtct ctactgtgcg gactaatttt ttagcactgt attgtacata
     1561 gtcgagtact tcggcaactg tttctccgtc aattatctgg tcgatgtggt accctttctc
     1621 attcactgaa acatgcactg cattggtgtc gccgaataag ttatgcaaat caccaaggat
     1681 ttcctgataa gcacctacaa gaaagacccc aatataatag ggctcggttg attttaattt
     1741 atgcaagggc aaatagtacg acacgttttt ggttgaaatg aaattatcga ttttcccgtc
     1801 cgagtcgcag gtaatgtctt gtatcgtagc attcttgatt ggtttctctt caagcctgtg
     1861 aataggcata atcgggaaaa tttgatcgat agcccacgag tcgggcaacg actggaacaa
     1921 cgagaagttg cagaaatact tgtcggccag catttttggc agctgtttca attcaaaagg
     1981 aatatgtttt atttcagcag ccatatcgaa aacttcgcgg gcaatgctcc agaatagccg
     2041 ctcaataagg gctcgggttt tcaggtcaag gaggttcagg gcaaaacggt caagcgattc
     2101 ttcgcgtatt tgctgtgcat cgtgccaact ttcgagcatc ccggtttggt ttaagttgtc
     2161 ccaaatttta tacatctctt gtaccagttc atggtcatcg gggtgaacag tgtctttttc
     2221 atcgtcccat tcgggtagtg ttgcagtttc aagcacttcg aataccaata ccgaatggtg
     2281 ggcagttagc gatcgtcccg attcggtgat gatgtttggg tgatgcaatc cgtttttgtc
     2341 gctgacgtcc acaaacgtac tcacagagtc gtttacatat tcttgtaccg aatagtttac
     2401 actgctttcg ctgttcgacg agcgggtgcc gtcgtaatcg acaccgaggc caccgcctat
     2461 atcgacaaat tcgataggga agcccaattt gcataattca gcatagaatt
//
LOCUS       DAOPUW010000268         2507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_43762_length_2507_cov_2.429445, whole genome shotgun sequence.
ACCESSION   DAOPUW010000268 DAOPUW010000000
VERSION     DAOPUW010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43762_length_2507_cov_2.429445"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1792
                     /locus_tag="PKJ43_05405"
     CDS             <1..1792
                     /locus_tag="PKJ43_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ72037.1"
                     /translation="EYYCQVINPNFPDLTISSMSFNYSFSLYTDSLALVALYNNTDGK
                     NWTNNANWCDTVPPLAPLSEWAGVTVNGNRVVKLVLTNNKLTGALPVEIGTLTQLTEL
                     SLHSNALSGNIPEEIGEIDSLRTLNLSNNLLDGPIPATIGQLSKLNSLSINSNQLEAL
                     PEEIGNLTNLNYLALNSNQLSGVLPASIGNLVNLKTLKLGSNQFSGALPADIGNLTSL
                     LDLSLEFNDFSGNLPGTLGDLLDLTSLTLNDNDFEGTLPEELSDLDYLTTLNVSGNHF
                     SGSISEDFSYLSNLNNFNISNNLYFFIDIEPVIDWCTDCGLVYSPQSKIGKENALVPG
                     YIGQPVMLEAEGYVVAEADEFTWFKNGVIINGETDSVLIIDSFNPANEGQYHCVVSNV
                     NVAGLLLESYPITLTEYSVPVDQVVANLSLASSADTCFNALQSITVAGSKAVTLQAGS
                     SATFIAGQSVRFLPGFHARAGSNAHAYITEDESFCEPEIPMAVYAALALKSAQGIEEA
                     VDNADGDNAMDMRVFPNPNNGSFTVRLPEGLSVQASVNVIDMQGRVVYSARGIGEPTF
                     DISLPNVQKGIYFVRVIDQETQLSKKVIVL"
     gene            complement(1972..>2507)
                     /locus_tag="PKJ43_05410"
     CDS             complement(1972..>2507)
                     /locus_tag="PKJ43_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72038.1"
                     /translation="PGTTSPIQQKTTTKHSYFAINALYDKYFEIRKRLTLGLLAEGVY
                     STRGLSSNYTEAIIAAPAFQPTPNSKSMYLEKYHANQYLATGGKILFKLNDNLHLRSE
                     VYGFFPIRDFVLGPDNSVSYHNDSFSRASFMGLAAAVYHTPLGPLSAEVNYYEKAGQK
                     WFFSLNLGYMIFNKRGL"
BASE COUNT          706 a          468 c          553 g          780 t
ORIGIN      
        1 agaatactat tgtcaggtta tcaatccaaa ttttccagac ctgaccattt cctctatgtc
       61 gttcaattac tctttttctc tttataccga ttcgctcgcg ttggttgcgc tttataataa
      121 tacagatggc aaaaattgga caaataatgc aaactggtgc gataccgttc ctccacttgc
      181 accattgagt gaatgggcag gggttactgt gaatggtaat agggttgtaa aacttgtgct
      241 tacaaataac aagcttaccg gtgctttacc tgttgaaatt ggaaccctta cacaactcac
      301 tgaattatcg ctgcatagta atgcactgag tggaaatata cccgaagaaa taggagaaat
      361 tgatagtttg cggactttaa accttagcaa taatttgctg gatggaccaa ttcctgcaac
      421 gattggccaa ttaagcaaat taaatagctt aagtataaat agcaatcaat tagaagcttt
      481 gcctgaagaa attggcaatt taaccaatct gaattatttg gcgttgaaca gtaaccaact
      541 ctcaggagtt ctacctgcaa gcatcggaaa ccttgttaac ttaaaaacat tgaagcttgg
      601 aagcaaccag ttttcaggag ctttgcccgc cgatattggc aacctgacaa gtttgcttga
      661 tttgtcgctt gaatttaatg attttagtgg aaatttgccc ggcacacttg gtgatctgct
      721 tgatttaact tcacttacat tgaacgataa tgactttgag ggaacgttgc cagaagaact
      781 ttcagatctc gattatttaa ccacgttgaa tgtttcggga aaccatttct ccggatcgat
      841 ttctgaagat ttcagttatt taagcaatct caataatttt aatatatcca ataatttata
      901 ttttttcatt gatattgagc ccgttattga ttggtgtact gattgtggct tggtttattc
      961 accacagtca aaaattggga aagaaaatgc tctggttcct gggtatattg ggcagccggt
     1021 tatgcttgaa gccgaaggat atgtggttgc agaagccgat gaatttacct ggtttaaaaa
     1081 tggggttata ataaacggcg aaacagattc tgttttaata atagattcat tcaacccggc
     1141 aaacgaaggt caatatcatt gtgttgtgtc taatgtaaat gttgcaggac ttttgctcga
     1201 aagttaccct ataaccctaa ctgaatactc cgttcctgta gatcaagttg tagccaactt
     1261 aagcctggca agttcggctg atacatgttt caatgccctt caatcgataa ccgtagcagg
     1321 aagtaaagcg gtaactttgc aagcgggttc atcggcaaca ttcatcgccg ggcaatcggt
     1381 acgttttctt cctggctttc atgctagggc cggaagtaat gctcatgcct acattaccga
     1441 agatgaatcg ttctgcgaac ccgaaatccc gatggctgtt tatgctgcat tggctctaaa
     1501 aagtgcccaa ggtattgaag aagctgttga taatgccgat ggagacaatg ctatggatat
     1561 gagggttttt cctaatccca acaatggttc atttaccgtt cggttacccg aagggctcag
     1621 tgttcaagct tctgtgaatg tgatcgatat gcagggtagg gtggtttatt cggcacgggg
     1681 aattggagaa ccaaccttcg atatctcgtt gcccaatgtt caaaaaggga tttattttgt
     1741 tcgcgtaatc gatcaggaaa cccaactgag caaaaaggtc attgtgctgt aggagagagc
     1801 gctgtaagga aagtggctat aatagaaaaa aggtttgatt acatagcgta gtcaaacctt
     1861 ttgtattgta gcatttttta tggttctaat attttatgct tggttttatc gtaggatatt
     1921 tcaactgact ttttgcaatg tagtcgtttt tattctgggt tggaacatcc cttataaccc
     1981 ccgtttgttg aaaatcatat agccaaggtt aagcgagaaa aaccattttt gtccggcttt
     2041 ttcgtagtaa ttaacttcgg cacttaacgg gccaagagga gtgtggtata ctgctgctgc
     2101 caagcccata aagcttgcgc gagagaaaga gtcgttatgg taacttacag agttatcagg
     2161 tccgagcaca aagtcgcgta tcgggaagaa accatagacc tcagagcgca aatgcaggtt
     2221 gtcgtttaat ttaaataaaa tttttcctcc tgtggccaaa tactggttgg catgatattt
     2281 ttcgagatac atcgatttgc tgttcggagt aggctgaaat gccggtgctg caatgatcgc
     2341 ttcggtatag tttgacgata gcccgcgggt actgtaaaca ccctcggcca aaagtcctag
     2401 tgtaagcctt tttcttattt caaaatactt atcgtacagt gcattgatag caaagtagct
     2461 atgttttgtt gtcgtttttt gttgaatagg cgaagtggtg cccgggt
//
LOCUS       DAOPUW010000269         2497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_44011_length_2497_cov_3.123260, whole genome shotgun sequence.
ACCESSION   DAOPUW010000269 DAOPUW010000000
VERSION     DAOPUW010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44011_length_2497_cov_3.123260"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..924)
                     /locus_tag="PKJ43_05415"
     CDS             complement(169..924)
                     /locus_tag="PKJ43_05415"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HNZ72039.1"
                     /translation="MSAFQILPGSPIIFAENFTVRSYHVDLHQRLTIHKLCSFFQDIA
                     GNHTVECGVGWDVMQQQQVFWVLSRMYIEVLSFPVWHDKISIKTWSMGTDGLYAYRNF
                     RVENERGDVLIKASSMWLMVNTETRRLVRPGEYMENFPLCTDRLLDTDPEKIPSLEEP
                     AFTGLSQVSFTETDMNMHTNNVSYIERIMNAYDFDFLMKKQVASFVINFIKEAVPGDM
                     LKVGSQQVSENEFINNIVRADGTEMVRTRIGWQ"
     gene            complement(995..1843)
                     /locus_tag="PKJ43_05420"
     CDS             complement(995..1843)
                     /locus_tag="PKJ43_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72040.1"
                     /translation="MKRIFSLLLMLTCFSSLAQKLDIRNSEFSFSIGTVEYNIEKFPN
                     SIDMDFLNYSNAYPGYFDYAVVNFGHKFDFLSKMSADINLIVMSNLVPDNYDVSVYYH
                     FNKVVALGLGSRLNKFNISYFEQFHLQGFPDYYLVDGNQRQFRVYDLGFYLTPMFKLI
                     RHERFQTTVKFDIGMSSFSEEEAVFYLKRKLSNERMAYHYYTTFSFQPYVNPKLELRL
                     RIFNINDTVVGLLLKSNFFYRYRSMNYNRSIQQWTCENEVYEFIRTPKHKCGRLEANA
                     GLYVKW"
     gene            complement(1847..>2497)
                     /locus_tag="PKJ43_05425"
     CDS             complement(1847..>2497)
                     /locus_tag="PKJ43_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72041.1"
                     /translation="DMDKKKSARRSLNASLPAKVFFVILVGVFTKLFFSCTPPPPGDM
                     YYNSIGIAGVDNSDRFLKRNTSVDTLYSGAVALNLTLKDSTFDYYAFNLNNALKSVSF
                     TPAMALSFPSSFIPVNEVEEIKVTTLFDIDAEVRAGDDITPLILYGTGRNFELYKNIG
                     NAISDFNKTQSATSVSIFLVLKRPIDNTMAQFYVQVFLDNDNVLSTNTNIFTIIKQ"
BASE COUNT          807 a          515 c          454 g          721 t
ORIGIN      
        1 tcaccagttc tcgaaacgcc aaatgacttg gttcaggcac atggaaaaga acgggaccac
       61 cattcattgg atcgacggga gtttgcccct cgatgagaaa ctgaagcaaa ttgagcgtct
      121 tgtctttccc aattaaaacg ttgcagcgaa ataaaataca aacaatattt actgccaacc
      181 gatgcgggta cgtaccatct ccgttccatc tgcacgaaca atattgttta taaattcgtt
      241 ttcggacact tgttggctcc caactttcaa catatcgcca ggaacggctt ctttaataaa
      301 atttataaca aacgaggcaa cctgtttctt cataagaaag tcgaaatcgt aggcattcat
      361 gatccgttcg atatagctaa cattattggt atgcatattc atatcggttt cggtaaacga
      421 aacttgcgac aagccggtaa acgcaggctc ttcgagtgac gggattttct cagggtcggt
      481 atcgaggagc cgatcggtac aaagcggaaa gttttccatg tattcacccg ggcgaaccag
      541 tcggcgagtt tcggtgttca ccatcaacca catactcgat gccttgatca acacatcgcc
      601 gcgttcgttt tcaaccctga aatttcggta agcataaagt ccgtcggttc ccatcgacca
      661 agtttttatc gagattttat cgtgccacac gggaaaagaa agcacttcaa tatacattct
      721 cgaaagcacc cagaaaacct gctgttgctg cattacatcc cacccaaccc cacactcaac
      781 cgtatggttt ccggcaatat cctgaaaaaa actacacaac ttatgaatgg taagccgctg
      841 atgcaaatca acatggtatg aacgaacagt aaagttttcg gcaaaaataa ttggtgaacc
      901 cggtaaaatt tgaaaagcac tcatagtgtt ggtttaaaaa cagggtaaaa gtaataatgc
      961 aaattaacaa caacaaccct gaacgcttac tatattacca ttttacataa agtccagcat
     1021 ttgcttctaa tcgaccacac ttatgttttg gtgttcgaat gaactcataa acctcatttt
     1081 cacaggtcca ttgctggatc gatcgattat aattcatact tctatacctg taaaaaaagt
     1141 tggactttag caatagcccc actactgtat catttatatt aaatattcgt aagcgtagtt
     1201 cgagtttggg attaacataa ggctgaaaag aaaaggttgt atagtaatga taagccatcc
     1261 tttcgtttga tagttttctt ttaagataga aaacagcctc ttcttctgaa aaagaggaca
     1321 ttcctatatc aaactttacc gtagtttgaa acctttcatg gcgtatgagt ttaaacattg
     1381 gtgtcaggta aaaacctaag tcatacactc taaactgcct ctggttaccg tcgactaaat
     1441 agtagtcggg aaaaccttgt aaatgaaatt gttcaaaata cgatatgtta aacttattaa
     1501 gccttgaccc aagcccaagc gcaacgactt tattaaaatg atagtatacc gaaacatcat
     1561 aattgtcagg aactaaattg ctcattacaa tcaggttgat gtctgcagac atttttgaaa
     1621 gaaagtcgaa tttatgtcca aaattaacaa cggcatagtc aaagtaaccc gggtaagcgt
     1681 tcgaatagtt taaaaaatcc atgtcaatcg aattcgggaa tttttcgatg ttgtattcta
     1741 ccgttcctat agaaaatgaa aattcgctgt tccgtatatc taatttctgc gctagcgagc
     1801 taaaacatgt caacataagt aataagctaa agattcgttt catgttttat tgctttatta
     1861 ttgtgaagat gttggtatta gtggaaagta cattgtcatt atctaaaaaa acctgaacat
     1921 aaaactgcgc catcgtatta tctattggtc gctttaatac aagaaaaatt gaaacactgg
     1981 ttgctgactg tgtcttgttg aagtccgaaa ttgcattacc gatattttta tataactcaa
     2041 aattccttcc tgtcccataa agaatcaggg gtgtgatgtc atcgcctgct ctcacttcag
     2101 catcaatatc gaacaaggtt gtaaccttaa tttcctcaac ttcatttacc ggaataaaac
     2161 tcgaaggaaa ggacaacgcc atagcgggag tgaatgatac ggatttcaac gcgttgttta
     2221 aattaaaggc ataataatca aacgtcgagt ccttgagggt aagattaaga gctacagcac
     2281 cggaatacaa tgtgtcaaca ctggtatttc ttttcaaaaa ccgatcgctg ttatcaactc
     2341 ctgcaatacc aatagaatta taatacatat cgccaggtgg aggtggcgtg caactaaaga
     2401 atagcttcgt aaataccccc actaaaatta caaaaaacac cttggcaggt aacgatgcgt
     2461 ttaaagatct ccttgcgctt ttctttttgt ccatatc
//
LOCUS       DAOPUW010000270         2496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_44046_length_2496_cov_3.077837, whole genome shotgun sequence.
ACCESSION   DAOPUW010000270 DAOPUW010000000
VERSION     DAOPUW010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44046_length_2496_cov_3.077837"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PKJ43_05430"
     CDS             complement(<1..304)
                     /locus_tag="PKJ43_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72042.1"
                     /translation="MEEYYVLIVGTIAIFVFVLIVSFFRRYKRCPSDRILVIYGKVGK
                     DKAQESRSAKCIHGGAAFIWPVIQDYSFMDLTPISIEINLTSALSKQNIRVDVPSRF"
     gene            complement(355..936)
                     /locus_tag="PKJ43_05435"
     CDS             complement(355..936)
                     /locus_tag="PKJ43_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72043.1"
                     /translation="MTTDFSLWWEGLSVLQKVYWLIAIPFSIFFILQTILSLFGGDSG
                     DSHGDADISVDSDTGIDFQFLSIKNLVAFFTLFGWVGILTSSGGMATGWSVLLAVIAG
                     LFMMVLMATLMYFMGKLTESGTLDLKKSVNKTGTVYLPIPPKRNGMGKVQITLQGFQT
                     LDAVTDEENEIPTGSLVEVTDTLDGEILVVKLK"
     gene            1217..1639
                     /locus_tag="PKJ43_05440"
     CDS             1217..1639
                     /locus_tag="PKJ43_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72044.1"
                     /translation="MKVLQFIGGIVFLFIFASCAHNAEIYNSGLPDGSVGMSYKVQKK
                     VTDKLLLEVINFEDTRCPVGEVCSNPGYVDVEIRASSGIDITSTTLRYNDFQPCDLSR
                     DTIFNYVVELVKVTPFPYADKPAQNAEIYRFAVVVEEL"
     gene            complement(1641..>2496)
                     /locus_tag="PKJ43_05445"
     CDS             complement(1641..>2496)
                     /locus_tag="PKJ43_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72045.1"
                     /translation="DEISRCPAALNQVKPVGYFHDQLSKALEMAISEQYNSGVQLLRS
                     QSINDARTAIEKFEWVKNAKPGYADVDNKLAQAIELATIKVVIEPIPYMGDQYTANIS
                     RFYSKLYADVSKNSRNRFVRFYQANEAEQMKIVPDQIVSMQFVDFAVGNVYEKETEKD
                     YVSDTLVAGTFKDDKGISHNVMGTVKAKAHIHEREVVSRGVLNVRVTRYPNKTILENR
                     NFPGEYVWRNQWATFNGDDRALPDDVKKMTKSRQTLPPLPQDLFVLFSDPLASNASSF
                     IKNYYRNK"
BASE COUNT          727 a          486 c          476 g          807 t
ORIGIN      
        1 taaagcgaga aggcacatca acgcggatgt tttgtttact caaagcactg gtcaaattaa
       61 tttctattga aatgggcgtt aaatccataa atgaataatc ttggataact ggccatatga
      121 acgcggcacc cccatgaata cactttgcag agcgcgattc ctgagctttg tcctttccaa
      181 cctttccgta gatgaccaaa atccggtcag aggggcatcg tttgtatcgc ctgaaaaatg
      241 atacaataag aacaaaaaca aatatggcaa tagtccctac aattaataca taatattctt
      301 ccatagtaaa aaaatttggg tttcgattta ttgtgacaat tgttattctg tgtcttactt
      361 gagtttcact actaggattt cgccatcgag cgtatcggtt acttcaacca gcgaaccggt
      421 aggaatttca ttttcttcat cagttacggc atcaagggtt tgaaagccct gaagcgttat
      481 ctgtactttt cccattccat ttcgcttggg tgggattggc aggtatactg taccggtttt
      541 gttcaccgat ttcttaaggt cgagggttcc cgattcggtt agttttccca tgaagtacat
      601 tagagtcgcc ataagtacca tcatgaacaa gccggcaatt acggccagca acaccgacca
      661 accggttgcc attcctcccg atgaggttaa tatgccaacc catccaaaaa gggtgaaaaa
      721 agctacaagg tttttaattg aaagaaactg aaaatcaatt cctgtatcgc tatcaacaga
      781 gatgtcggca tctccatggc tatcgccact gtcaccgcca aagagtgata atattgtttg
      841 tagaataaag aaaatggaaa atggaatggc aattagccaa tatactttct gtagaacaga
      901 taagccttcc caccataatg aaaaatcagt cgtcataagc gttgtgttta gtttctgttt
      961 aaaagtaaac agaaattttg aatcttttca acaaaaaata ttttttctta gttagtcggg
     1021 cattttctta aagcctttac atatagtttt gtagttctat ttatttcatt aaatccatat
     1081 aatgcacaga atatattaaa acatctttac tcctttcaat atgcatacct aatgcagaaa
     1141 aaaagttttt atgctgtttg acagtttttt aaaagaaaat catatatttg tgatgggaga
     1201 attttttaga ttaagcatga aagtattaca atttattggg ggaattgtat ttcttttcat
     1261 ttttgcttcg tgtgcgcaca atgcagagat atacaatagt ggcctgcccg atgggagtgt
     1321 tgggatgagc tacaaagttc aaaaaaaagt aaccgacaag ttgcttcttg aagtcattaa
     1381 ttttgaagat acccgttgcc cggttggcga agtttgcagt aaccctggct atgttgatgt
     1441 agagatacgg gcctcttctg gtatagacat cacaagtaca acccttcgtt ataacgattt
     1501 tcagccttgc gatttaagcc gcgacactat ttttaactat gtggttgagt tggttaaggt
     1561 aactccgttc ccttatgctg acaagcctgc tcaaaatgct gaaatatacc gcttcgcagt
     1621 ggttgttgaa gaattataat ctatttgttt cggtaataat tcttaatgaa cgatgaagca
     1681 tttgatgcta acgggtcgct aaataacaca aacaaatctt gtggtaatgg tggtaaggtc
     1741 tgtcgcgatt ttgtcatttt ctttacatca tcgggcagcg cacggtcatc tccgttaaat
     1801 gttgcccatt ggtttcgcca tacatattcc ccggggaagt tgcggttttc aagaattgtt
     1861 ttattgggat acctagtaac ccgtacgttt aagacacctc tcgaaacaac ttcacgttcg
     1921 tgaatatgtg cttttgcttt cactgttccc atcacattgt gtgaaatgcc tttgtcatct
     1981 ttaaatgttc ctgcaaccaa tgtatctgat acataatctt tttctgtttc tttctcgtat
     2041 acatttccaa cggcaaagtc aacaaattgc atcgacacaa tctggtctgg aacaatcttc
     2101 atttgttcag cttcgttggc ctgataaaac cttacaaaac ggttccgtga atttttcgaa
     2161 acatcggcat aaagttttga ataaaaacga cttatgttag cagtatattg atcgcccata
     2221 tagggtatag gttcaattac gaccttaata gtggctaatt caatggcttg cgctagttta
     2281 ttgtcaacat cagcataccc aggttttgca ttttttaccc actcaaattt ttcgatagca
     2341 gttcgagcat cattaatact ttgacttcga agtaattgaa cgccggaatt atattgttct
     2401 gaaatggcca tttcaagtgc cttgcttaat tgatcgtgaa aataaccaac tggctttacc
     2461 tgattgagtg ccgccggaca acgtgaaatt tcgtcg
//
LOCUS       DAOPUW010000271         2490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_44209_length_2490_cov_3.313347, whole genome shotgun sequence.
ACCESSION   DAOPUW010000271 DAOPUW010000000
VERSION     DAOPUW010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44209_length_2490_cov_3.313347"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1161)
                     /locus_tag="PKJ43_05450"
     CDS             complement(94..1161)
                     /locus_tag="PKJ43_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNZ72046.1"
                     /translation="MSIQINGIQQLGVGVVNTDESFAWYRRHFGVDIEVVNEKAMAEY
                     MLPYTGGQPRKRHTVLAMNLQGGGGFEIWQHTGREPLSPNFEVKIGDLGISAGKLKTT
                     DIEKTYDAFKKKGLNLLTSITTAPSGRAHFFMKDAYNNLWEFIEYQEFFTKTAAVNGG
                     VLGAIIGVKNMEESLSLYRDILRYDKTIYDKTAVFNDFEGIPGGENNYRRVLLTHSDK
                     KSGPFSPIFGSSEIELVEVLDRQAIKIYEDRMWGDPGFIHLCFDVHNMNAIREELNSK
                     GFPFTVDSAKQIETFDMGDAAGNFAYIEAPEGTLIEFVETLKVPIMQKWGIFIDLRKR
                     SPHKPLPRIILKAFSLKRVHE"
     gene            complement(1181..1975)
                     /locus_tag="PKJ43_05455"
     CDS             complement(1181..1975)
                     /locus_tag="PKJ43_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNZ72047.1"
                     /translation="MDFRGKTFWITGAASGMGRALAIKLATYQARLIISDRDEAGLVA
                     TAKEITSRNSTVRIEPLDMLDAQAIEQTAKRVIADGEKISGLYQFAGISQRSLLIDTP
                     MENIRKIMEVNFFGVVAHTKAVLPHMIENGGGQLAATSSIVGKFGFPYRTAYSASKHA
                     IHGFFESLLAENSKYNIKTSVLIPGRVQTNISKFALEKDGSEHGVIDPGQANGISAEK
                     AANMIFNGLKKEKKEIWVGASEMQMVNIRRFLPWLYYKLAPRIKPM"
     gene            complement(2029..>2490)
                     /locus_tag="PKJ43_05460"
     CDS             complement(2029..>2490)
                     /locus_tag="PKJ43_05460"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNZ72048.1"
                     /translation="FLNNFTANGTFAKTRTLIHHGNKKSNQIFQNDRFAAHSGIKVVE
                     ASEGYCKAVMEISDFHLNAAGVVQGGAIFTLADFAFGLASNSSGQLALAINVNITFLK
                     GKQSGTLFAIARQLDDPKRIGAYTVSVTDENDQLIATFNGLCYRKKETIGG"
BASE COUNT          622 a          554 c          499 g          815 t
ORIGIN      
        1 ctcgatgcaa tgatgacgta agtcatctgt tgttcatcta cttttccctg atcgaaaatt
       61 ttcaccattt ttgttgaaac aaattctgta aagttactcg tgcactcttt tcaaactgaa
      121 tgctttaaga ataatccggg gaagaggttt gtgtggcgat cgttttctta ggtcgataaa
      181 aattccccat ttctgcatga tgggaacttt cagtgtttca acaaattcga tcaaagttcc
      241 ctctggagct tcaatatatg caaagttccc ggcggcatcg cccatgtcga atgtttcaat
      301 ttgctttgcg ctgtcaacgg taaacggaaa tccttttgaa ttcagctctt ccctgattgc
      361 gttcatgttg tgcacatcga aacacaaatg gataaatccg ggatctcccc acatccggtc
      421 ttcatatatt tttatggctt gtctgtcaag aacttcaacc agttcaattt ctgagctccc
      481 aaaaatgggg ctgaatggtc cgcttttttt atcactatgg gtcagaagca ctcttctata
      541 attgttttca cctcccggaa tgccttcaaa gtcgttgaaa acagcagttt tgtcatatat
      601 tgttttgtcg tatctcagga tatcacgata taacgacaag ctttcctcca tattctttac
      661 cccaataata gcccccaata cacctccgtt aacggccgct gttttggtga agaattcctg
      721 atattctata aattcccaaa ggttattgta agcgtctttc ataaagaagt gggctcttcc
      781 cgatggggca gtagttatgc tcgtaagaag attgagcccc ttttttttga atgcatcata
      841 agttttttct atgtcggtgg ttttcagttt tcctgccgat atacccaaat cgccaatttt
      901 aacttcaaaa tttggactaa ggggttctcg tcctgtgtgt tgccaaatct cgaagcctcc
      961 tccgccttga aggttcattg caaggactgt gtgccgtttt cgaggttgcc ctcctgtata
     1021 tgggagcata tattcggcca tagccttttc gttaacgact tcgatatcaa caccaaaatg
     1081 gcgtcgatac catgcgaaac tttcatcggt atttacaact ccaaccccta attgctggat
     1141 cccgtttatt tgaattgaca tagattatac ttttttgttt ctacattggt ttaattcgtg
     1201 gtgccagttt gtaatagagc catggcagga aacggcgtat attgaccatt tgcatttcgg
     1261 aggcacctac ccaaatttct tttttctctt tttttagccc attgaaaatc atatttgcag
     1321 ctttctctgc acttattccg ttggcttggc ccggatcaat tactccgtgt tccgagccgt
     1381 ctttttctaa agcaaatttt gatatgttgg tttgaaccct gcccgggatg agtacactgg
     1441 tttttatatt gtatttcgaa ttttcggcga ggagactttc aaaaaaaccg tgtatagcat
     1501 gcttcgatgc ggaataggct gtgcgatagg gaaaaccgaa ttttcctacg atgcttgatg
     1561 ttgcggcaag ctggcctcca ccattctcaa tcatatgtgg cagcacggct tttgtatggg
     1621 caactactcc aaagaaattc acttccatta ttttcctgat gttttccata ggggtatcaa
     1681 ttagaagtga gcgctggctg atgcctgcaa attggtacag accagagatt ttttcgccat
     1741 ccgcaataac gcgcttagct gtttgttcta tggcttgggc gtcaagcatg tcaagaggtt
     1801 caatccgaac agtactattt cgtgaagtga tttcctttgc agtagccact aagccggctt
     1861 catcgcgatc ggagatgatt aagcgagctt gatatgtggc taactttatc gcgagagcgc
     1921 gccccatgcc cgatgcagca ccggttatcc aaaatgtctt tcctcgaaaa tccatacgca
     1981 ttttttgttt gaaacgaatg tatgaataat tgtgcgaaaa ccattgtttt acccgccaat
     2041 tgtctctttc ttacgataac acaatccgtt gaaagtggca attaattggt cgttttcgtc
     2101 ggttacgctt acagtatagg ctcctattcg tttcgggtcg tcaagctgcc gggcaatagc
     2161 aaaaagagtt cccgactgtt ttcctttcaa aaatgtaatg ttcacattta tagccaacgc
     2221 gagttgtccc gagctgttcg acgctaagcc aaaagcaaaa tcggccagtg taaaaattgc
     2281 tcctccctga accacgcctg ctgcattcag gtgaaaatca gaaatctcca ttactgcttt
     2341 gcaatagcct tccgatgctt caactacttt tatccctgaa tgagcggcaa agcggtcgtt
     2401 ttgaaaaatt tgattacttt ttttatttcc atggtgtata agtgttcgag ttttcgcaaa
     2461 agtaccattc gcggtaaaat tgttcaagaa
//
LOCUS       DAOPUW010000272         2458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_45034_length_2458_cov_4.104037, whole genome shotgun sequence.
ACCESSION   DAOPUW010000272 DAOPUW010000000
VERSION     DAOPUW010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45034_length_2458_cov_4.104037"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..233)
                     /gene="rpsU"
                     /locus_tag="PKJ43_05465"
     CDS             complement(42..233)
                     /gene="rpsU"
                     /locus_tag="PKJ43_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNZ72049.1"
                     /translation="MIVIPVKEGENVERALKRFKRKFEKTGVIKELRSRQAFEKPSVI
                     RRAEIKKAIYIQSLQREDD"
     gene            complement(333..1247)
                     /locus_tag="PKJ43_05470"
     CDS             complement(333..1247)
                     /locus_tag="PKJ43_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNZ72050.1"
                     /translation="MKIKRFLDPFILLSKAERNGAIVLLIIIGVLMITRILLPRFFKP
                     GGKDIPVLEKRLELVQKQNDSLDALRPKKKKSHSNYPDNSANKAKQVGNKDFQRSPSV
                     LFVFDPNNVTLTEMDSLGFSKYAAQNLINYREKGGKFYKPEDVKKIYGVDSALFAVLL
                     PYITVESASKQKILLELNSSDSISLIALNGIGPKLASRICKYRKQLGGFVRLEQLKEV
                     YQFSDETYNAVIPSLTVDPNLAKKINVNFADINEMKNHPYIDYKMARKIVDYRSSKGF
                     IKSVDVLLRDSILDQQAYDKIHAYLKIE"
     gene            1479..>2458
                     /locus_tag="PKJ43_05475"
     CDS             1479..>2458
                     /locus_tag="PKJ43_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HNZ72051.1"
                     /translation="MAVSYKELGLVNTKELFKKAIEGKYAIPAFNFNNLEQMQAIIAA
                     CVETKSPVILQVSSGARKYANQTLLRYMAQGAVEYAKELGYAIPVVLHLDHGDSFELC
                     KDCIDMGFSSVMIDGSHFSYEENIALTKKVVEYAHAHDVTVEGELGVLAGVEDEVSSE
                     HHTYTRPEEVVDFVTRTGVDSLAISIGTSHGANKFTPAQCTRDENGILIPPPLRFDIL
                     EEIEKQIPGFPIVLHGSSSVPQEYVETINKYGGALKDSIGIPEDQLRKAAASAVCKIN
                     IDSDGRLAMTAAVREVMATKPGEFDPRKYLGPARDELKKLYMHKTVNVLGS"
BASE COUNT          742 a          480 c          452 g          784 t
ORIGIN      
        1 taacaataat gccgggataa agttccggac gaaaccctca attaatcatc ttcgcgttgc
       61 aggctctgga tgtagatagc tttcttaatt tctgctcttc taataacaga aggtttttca
      121 aaagcctggc ggctgcgtaa ctctttgatt acacctgttt tttcaaattt acgtttgaag
      181 cgtttcaacg ctctctcaac attttcgcct tctttaactg gaataacaat catatttctt
      241 tactgttaat ttcttttttc tttcaaaacg gagcgcaaat ttagtaatga ttcaattata
      301 ttcaaatttt ttcagaaaaa agaaaatgtc tactattcga ttttcaaata ggcgtgtatt
      361 ttatcatatg cttgctggtc gagaattgaa tcacgaagta acacatccac tgattttata
      421 aagcctttgc tggaacggta atcgacaatt ttccgggcca ttttataatc gatataagga
      481 tggttcttca tttcgtttat gtcggcaaaa ttaacattga ttttctttgc caaattggga
      541 tctactgtaa gtgatggaat aactgcattg taggtttcat cagaaaactg ataaacttct
      601 tttagctgtt ctaagcgaac aaatccgccc agctgtttcc tgtacttgca aatacgagaa
      661 gccagcttgg gacctattcc attaagcgct atcaaggata ttgaatcgga agaattcaac
      721 tctaaaagaa tcttctgttt ggaagcgctt tcaacagtta tatatggtaa aagaaccgca
      781 aacaatgcgg agtcaacacc gtaaatcttt ttcacgtctt cgggtttata gaacttgccc
      841 cctttttctc tatagtttat taagttctga gcggcatact tagaaaatcc caaactatcc
      901 atctcagtca atgttacatt attaggatcg aacacaaaca gcaccgaagg actacgctga
      961 aaatctttgt tcccaacttg tttcgctttg ttggctgaat tatcgggata atttgaatgg
     1021 gatttctttt tcttagggcg taaagcatcc agactatcgt tttgtttttg aactaattcc
     1081 aagcgtttct ccaatacagg aatatctttc cctcccggtt taaagaagcg aggcaaaagg
     1141 atccttgtaa tcataagcac cccaataatt attaataaaa caatggctcc gttacgttcg
     1201 gcttttgaca acaagatgaa tgggtcgaga aatcttttga tcttcatact agtttttttc
     1261 aaaagtcgga atttttagga taacttgcat cggttataaa aaatttgtta aggattgatt
     1321 tccaaatcat gcctaagaca aaagcacgaa gttttttaaa cattaatgac taaacttgca
     1381 gaaaatgcaa ttaaagataa aattgagttt ttcaagctta ttcgtatttt tgcatttctt
     1441 tagaaaattt tgttagtact aattaaataa tatacaaaat ggcagtaagt tataaagaac
     1501 tcggtttggt gaataccaaa gagttgttca aaaaagcaat tgaagggaaa tacgctatcc
     1561 ctgcgttcaa cttcaataac ttggagcaaa tgcaggcaat tatagcagct tgtgttgaaa
     1621 caaagtctcc tgtaatcctt caggtttcga gtggtgcacg taaatatgca aaccaaacct
     1681 tgcttcgcta tatggcacaa ggtgcagttg aatatgcaaa agaacttgga tatgctattc
     1741 ctgttgttct tcatctcgac cacggcgata gttttgaact ttgcaaagat tgtatcgaca
     1801 tgggtttttc gtcagtaatg attgacggtt ctcacttctc atacgaagaa aatattgccc
     1861 tcacaaagaa agttgttgag tatgcacatg ctcacgatgt tactgttgaa ggcgaacttg
     1921 gcgtgttggc cggtgttgaa gacgaagttt cttcagaaca ccacacatac acccgccctg
     1981 aagaagttgt tgattttgtt acccgcaccg gtgttgattc attggctatt tcaattggta
     2041 catcacacgg agccaacaaa ttcactcctg cccagtgtac tcgcgatgaa aatggcattt
     2101 taattccacc tccattgcgc ttcgacattc ttgaagaaat tgaaaaacaa attccaggtt
     2161 tcccaatcgt tctccacgga tcttcatcgg ttccacagga atatgttgaa acgatcaaca
     2221 aatatggtgg cgcattgaaa gattctattg gaatccctga agaccaactt cgcaaagcag
     2281 ctgcttctgc tgtttgcaaa atcaatatcg attccgacgg ccgtcttgca atgactgctg
     2341 cagttcgtga agttatggct acaaaaccag gagaattcga tcctcgtaaa taccttggtc
     2401 cagcacgtga cgaactgaaa aagctttaca tgcacaaaac tgtaaatgtt ttaggttc
//
LOCUS       DAOPUW010000273         2448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_45332_length_2448_cov_1.623903, whole genome shotgun sequence.
ACCESSION   DAOPUW010000273 DAOPUW010000000
VERSION     DAOPUW010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45332_length_2448_cov_1.623903"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PKJ43_05480"
     CDS             complement(<1..502)
                     /locus_tag="PKJ43_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HNZ72052.1"
                     /translation="MAVKKTTGNRKPNPTAMVAMDMGKVPPQAIELEEAVLGAIMIER
                     DAVIAVIDVIKVESFYKESHQKIFQAVLDLSSRHEPIDLLTVTEELRKRDELDAVGGA
                     VYLAQLTSKVGSAAHLEFHSKIIAQKFIQRELIRVSSDIQSKAFDDSIDVDYLLDYSE
                     MELFRVA"
     gene            801..1874
                     /gene="corA"
                     /locus_tag="PKJ43_05485"
     CDS             801..1874
                     /gene="corA"
                     /locus_tag="PKJ43_05485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNZ72053.1"
                     /translation="MITFRTLRKTIPLNRKKSDPDKFVFTGVYPVSTINIQLLQYNKD
                     ECLEKRDIPPEGVDGFEDKGYIHWLNIYGIDNADTIASICKRQGIHDLVIQDILDVNQ
                     RPKFQEFENYSFLTLKSIAPSDNDIVTEQISFVFGSNFLISFQEKEASFFEHVRHMLR
                     DGKGIIRERGADYLLYALLESILDNYFKTLNRLDNEIEKYNFSDINKDLSPHLLANIE
                     SNKKFAQYIKKSIFPIKEFSLIAERGEYQWIDRRSIKYFLEIKDLCLTLMDGCDMLLW
                     SLDSSTNLFFYIQGHRMNQVMKTLTVVATIFIPLTFIAGIYGMNFRNMPELSWQYGYL
                     TTWVAFAFIVLGMLFYFRKKKWF"
     gene            1887..>2448
                     /locus_tag="PKJ43_05490"
     CDS             1887..>2448
                     /locus_tag="PKJ43_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HNZ72054.1"
                     /translation="MLQTVLLLTFGFAILIKGADWLVDGSSALARKYHVSSLVIGLTI
                     VAFGTSAPELVVNIFASFQNHQDIVFGNIIGSNIFNLFAILGIAGMITPLVVQSSTVW
                     KEIPISFLATIILFVMAMDFLLPHHSMLSRIDGAIMLLLFGLFLYYVFTQIKNSNTAE
                     SEKHKQVSTLRIWLQIILGLTGLIAGG"
BASE COUNT          704 a          556 c          492 g          696 t
ORIGIN      
        1 cggccaccct aaaaagctcc atttcggagt agtctaacag gtagtcaaca tcaatggaat
       61 cgtcgaaagc tttgctttgt atatcgctgg aaacccttat cagctcacgc tgaataaact
      121 tctgtgcaat gattttggag tgaaactcca ggtgtgccgc cgaacccacc ttcgaagtaa
      181 gctgtgcaag gtaaaccgca ccgcccaccg catccagttc atcccgcttg cgcagttcct
      241 cggtaacggt caacaagtca atcggttcat ggcgtgagga caggtctaat acggcctgaa
      301 aaattttctg gtgactctcc ttataaaaac tttcaacctt gattacatcg ataaccgcaa
      361 taacggcgtc cctttcgatc ataattgccc caagtaccgc ctcctccagc tcaatggcct
      421 gtgggggtac ttttcccatg tccatcgcca ccattgccgt gggattaggc ttacgattcc
      481 ctgttgtttt ttttactgcc atcttctatc ttttttgcaa aggtaacccc caaatacctg
      541 ttttatggaa agaaactgat gcgggttatt aacaaattac tttggagttt tttcacaatt
      601 tgttaaaaaa tggttttaaa aaaatataac cacagggtta aagcacaagc ccaaaagatt
      661 ttacatacct ttgtgcaata atggctttcc gacaaagaat ttcattatat gatcaccatt
      721 gtataacatt ttcaacacgt cgaaattcac acacatagtt gactacaaag caattgaaag
      781 aataactaaa gatagaaaaa gtgataacct tccggacgtt aagaaaaacc atacccctca
      841 atcgcaaaaa aagcgatccc gacaagtttg tttttactgg agtttacccc gtttctacaa
      901 tcaatattca acttttgcaa tacaataaag atgagtgcct tgagaaaagg gatattccgc
      961 ccgaaggggt tgatggtttt gaagataaag gctacatcca ctggctaaat atttacggca
     1021 ttgacaatgc cgacaccatc gcctccattt gcaaaaggca aggtattcac gacctggtga
     1081 ttcaggatat tctggatgta aatcaaaggc ccaaattcca ggagtttgaa aactactcgt
     1141 tcttaacgct aaaatccatt gccccttccg acaatgacat tgttaccgaa cagatcagct
     1201 ttgtttttgg atctaatttt ctcatatcgt ttcaggaaaa ggaggcctcc tttttcgaac
     1261 acgtaaggca catgcttcgc gatggcaagg gcattatcag ggagcgcggg gccgactacc
     1321 tgctgtacgc gctgctggaa tcgattctgg ataactattt caaaacgctg aacaggctgg
     1381 ataacgaaat tgagaagtac aatttttcag atattaacaa agatttatcg ccccatttgc
     1441 tggccaatat cgagtccaac aagaaattcg cacagtacat taaaaagtca atttttccga
     1501 ttaaagagtt cagcctgatt gccgaaaggg gcgaatacca gtggattgac agaagaagca
     1561 ttaaatactt tcttgaaatt aaagacctat gcctaaccct catggatgga tgcgatatgc
     1621 tcctatggtc gctggatagt agcacgaacc tgttctttta catacagggt caccgtatga
     1681 atcaggtgat gaaaacctta accgttgtgg caaccatctt cataccatta acgttcattg
     1741 ccggaatcta tggcatgaat ttccggaaca tgcccgagct gtcgtggcaa tatggatacc
     1801 taaccacatg ggttgctttt gccttcatag tgttgggaat gctgttctat ttcagaaaga
     1861 aaaaatggtt ctaacacaac tggcccatgc tgcaaaccgt tctgttactt acctttggat
     1921 ttgccatcct gataaaaggt gccgattggc tggttgatgg ctcttcggcc ctggcacgaa
     1981 aataccacgt atcgagtttg gtcatcgggt taaccattgt tgccttcggc acttctgccc
     2041 ccgaactggt ggtaaatatc tttgcctcat tccaaaacca tcaggatatc gtttttggaa
     2101 acatcatagg cagcaatatc ttcaacctat ttgccatcct gggaattgcc ggcatgatta
     2161 cacctttggt ggtacaatcg agcactgtat ggaaagaaat acccatctcc tttttggcca
     2221 cgattattct atttgtcatg gcaatggatt ttttattgcc tcaccattcc atgctctcga
     2281 ggattgatgg ggcaatcatg ctgctgttat tcggcctttt tctctactac gtttttactc
     2341 agattaaaaa tagtaacact gccgaaagcg agaaacataa gcaggtatcg accctcagaa
     2401 tttggctgca gattatatta ggactaacgg gattgatagc cggaggac
//
LOCUS       DAOPUW010000274         2445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_45400_length_2445_cov_4.896653, whole genome shotgun sequence.
ACCESSION   DAOPUW010000274 DAOPUW010000000
VERSION     DAOPUW010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45400_length_2445_cov_4.896653"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PKJ43_05495"
     CDS             complement(<1..625)
                     /locus_tag="PKJ43_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNZ72055.1"
                     /translation="MKTICLYFQLHQPFRFRRYRFFDIGNDHYYYDDYANETILRKVA
                     DNCYLPANKVILEAIKKHKGKFRVAFSLSGIAIEQFLLYAPEVIQSFKELADTGCVEF
                     LSETYSHSLVSLKSKEAFQHQVEKHDNLIEKYFGMKPSVFRNTEMIYSDEIGSMVADM
                     GFKAILTEGAKHVLGWKSPNFMYTNAINPRLKVLMRNFKLSDDLSFRF"
     gene            complement(622..1929)
                     /locus_tag="PKJ43_05500"
     CDS             complement(622..1929)
                     /locus_tag="PKJ43_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ72056.1"
                     /translation="MMKVLMFGWEFPPHISGGLGTACYGLTKGLSGFDDIEIIFVVPK
                     AWGDEDQSSMKLIGAGDIPVTRKKIKFDDIQQKIDYFEVDSMIVPYVDPDEFWKLSSI
                     KRSSKTRFVETDDFGKIKFTGQYGADLISEIYNYALVAETIASDNEFDIIHAHDWLTY
                     PAGIAAKRISGKPLVIHVHATDFDRSGGSVNPKVYAIEREGMEVADKIITVSNLTKQI
                     VIEKYGIDPSKVVTVYNAVEPVTFDTRKALKRGIDDKIVTFLGRITMQKGPEYFIEAA
                     HKVLKRTKNVRFVMAGSGDMMNAMVRRAAALRITDHFYFTGFLRGDDVFNMFKMSDVY
                     VMPSVSEPFGISPLEAMQSDVPVIISKQSGVAEILTHAIKIDFWDINAMADAIYALVT
                     YPALSREFKKQGRTEVDSLQWKNSAKNVRDVYMNTLNLINKKI"
     gene            complement(1944..>2445)
                     /locus_tag="PKJ43_05505"
     CDS             complement(1944..>2445)
                     /locus_tag="PKJ43_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNZ72057.1"
                     /translation="YLGDCCFQGTCDWTIRPNQVIAAALYYSPLSKEMKKSVLSLAKK
                     DLLTPRGLRTLSPENERYMGSYEGNEQQREEAIHQGSIFPWLIGFFIEGYLKIHKRGG
                     LAFTKTIMEGFEEEMTQHCIGTISEVYNGNPPHTGKGSVSQAWNIGEILRAYHLIDTY
                     ESENQN"
BASE COUNT          701 a          543 c          454 g          747 t
ORIGIN      
        1 aaaaccgaaa tgaaagatcg tcgcttagct tgaaattacg cataagcacc tttaatcgag
       61 gatttattgc attggtatac ataaagttgg ggctcttcca gccgagaaca tgctttgctc
      121 cttcagttag tatagcttta aagcccatat cggcaaccat gcttccaatt tcgtcggaat
      181 agatcatttc agtgttacgg aaaacggatg gcttcattcc aaaatatttc tcgataagat
      241 tatcgtgctt ttcgacctga tgttgaaagg cttctttgct tttcaacgaa accaaagaat
      301 gggaataggt ctcggaaaga aactcaacac aaccggtatc ggctagctcc ttaaacgatt
      361 gtataacctc gggtgcatat aacaaaaact gttctatggc aatgcccgat agcgaaaaag
      421 ctacacgaaa tttgccttta tgcttcttta tcgcctcgag aataaccttg ttggcaggca
      481 gatagcaatt gtcggccacc ttgcgcaaaa tggtttcatt ggcgtaatcg tcgtaataat
      541 aatgatcgtt ccctatatcg aaaaaacgat aacgacgaaa cctaaaaggc tgatggagtt
      601 gaaaatagag gcaaatagtc ttcatatctt tttattaata aggttcaaag tattcatata
      661 tacatcgcgg acattttttg cagaattttt ccactgcaag ctgtcaactt cggttctacc
      721 ctgcttttta aattcacggg ataaagcggg gtaagtaacc aaagcataaa ttgcatcggc
      781 catggcattg atatcccaaa agtcgatttt tatggcgtgg gttagaattt ctgctacccc
      841 cgactgtttt gaaatgatta ccggtacatc agattgcatt gcttcgagcg gagatatgcc
      901 aaaaggctca gacaccgaag gcattacata cacatcgctc attttaaaca tattaaacac
      961 atcgtcgcca cgaagaaatc cagtaaaata gaaatggtcg gtaattcgta atgcagcagc
     1021 gcgcctcacc atggcattca tcatatcgcc acttccggcc atcacaaaac gtacattctt
     1081 tgtccttttc aaaactttat gagcagcttc aataaaatat tcaggccctt tttgcatggt
     1141 aatacgccca aggaaagtaa caatcttatc atcaattcct cgctttagtg ctttgcgggt
     1201 gtcgaatgtt accggctcaa cagcattgta cacagtgaca accttcgaag gatcgatccc
     1261 atatttttca attacaatct gtttagtaag gttacttacc gttattattt tatcggctac
     1321 ttccatccct tccctttcga tggcataaac cttagggttc acgcttcctc cacttcggtc
     1381 gaagtcagta gcatggacgt gtattactaa cggcttccct gatatccgtt tagctgcaat
     1441 cccggcagga taagtcagcc agtcgtgcgc atggatgata tcgaattcat tgtctgaagc
     1501 aatagtttcg gcaactaatg catagttata aatttctgaa ataagatcgg caccgtattg
     1561 tccggtgaat ttgattttac caaaatcgtc agtttcaaca aaacgggttt ttgaagaacg
     1621 ctttattgaa ctcagtttcc agaattcatc aggatcgaca taggggacaa tcatagaatc
     1681 gacctcaaaa taatcgattt tttgttgtat gtcgtcgaat ttgatttttt tgcgggtaac
     1741 cggaatatct cctgccccaa taagtttcat cgagctctga tcttcgtcgc cccatgcttt
     1801 tggcacaaca aaaattattt caatatcatc gaaacccgac agtccctttg taagcccgta
     1861 acaagctgtt cccaaaccac ccgaaatatg gggcggaaat tcccaaccga acattaacac
     1921 cttcatcatc aaatctttat tagtcaattt tggttttcac tttcatacgt atcaattagg
     1981 tgatacgctc tcagtatttc gcctatgttc catgcttggg atactgaacc ttttcctgtg
     2041 tgcggaggat ttccgttgta tacttcagaa atagtcccga tacaatgctg ggtcatttct
     2101 tcttcaaatc cttccattat agttttagta aatgctaagc ctccacgttt atgaattttc
     2161 agatagcctt cgataaaaaa gcctatcaac caaggaaata tcgagccctg gtgaattgct
     2221 tcttcccgtt gttgctcatt tccttcgtaa ctacccatat atctttcatt ttcaggcgat
     2281 aatgttctaa gtccacgtgg agttagtaaa tcttttttgg ccaggctcaa caccgatttc
     2341 ttcatttctt tggaaagagg tgaataatat aatgctgcag caataacctg gttgggcctt
     2401 attgtccaat cacaggttcc ctgaaagcaa caatcgccca gatat
//
LOCUS       DAOPUW010000275         2444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_45428_length_2444_cov_3.867727, whole genome shotgun sequence.
ACCESSION   DAOPUW010000275 DAOPUW010000000
VERSION     DAOPUW010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45428_length_2444_cov_3.867727"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..668)
                     /locus_tag="PKJ43_05510"
     CDS             complement(51..668)
                     /locus_tag="PKJ43_05510"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNZ72058.1"
                     /translation="MFSGIVEEAGKVIAIERDQENYHFTLTCSFIDELKIDQSLSHNG
                     VCLTVVKIDKEKKTYVVTAIKETLLKSNLGLLEPGCEVNLERSMIMNGRLDGHIVQGH
                     VDQTAECTQVEEADGSWYYTFEYPFDKEKARQGYMTVEKGSVTVNGVSLTVVNSKLNS
                     FQVAIIPYTYHHTNFHAIEKGTKVNLEFDIIGKYLSKLMVINNLD"
     gene            complement(778..1269)
                     /locus_tag="PKJ43_05515"
     CDS             complement(778..1269)
                     /locus_tag="PKJ43_05515"
                     /inference="COORDINATES: protein motif:HMM:NF025160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HNZ72059.1"
                     /translation="MRNLSYNKALLASGIIFTIHNCEEAIGYALFMPEIGSRLPIAVT
                     KTNPMIAAIIAITLIAWAIISWGLSSRNERLQRDILTLITTVFFVNAFIPHIILTLLL
                     MNYTPAVITSIVLYLPFSVYILPKLYSTFNPKNSFFKLIGIWALPLCALVGGLQLAMS
                     LIL"
     gene            complement(1321..1806)
                     /locus_tag="PKJ43_05520"
     CDS             complement(1321..1806)
                     /locus_tag="PKJ43_05520"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ72060.1"
                     /translation="MIISFATEHYAKSFCKSLDIVARERKYLGSTTGFSEESTVNFVK
                     FIVENNHAQYYAIDKETVVGWCDIIPKSYEGLTHVGVLGMGVLKEYRNQGLGSQLLEK
                     TLAHAKTNGLEKVELEVYESNTAAVNLYIKFGFEVEGKRIKSRKLDGCYDNIIQMGKF
                     L"
     gene            2099..>2444
                     /locus_tag="PKJ43_05525"
     CDS             2099..>2444
                     /locus_tag="PKJ43_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72061.1"
                     /translation="MKKVYINQGIATSDFSLGKKCFSSFASKNSIVHFLGRKKKVATM
                     LVFAFFALFMVSCDLLNDEGKSFDKSLLYGTWRSGTLYYSYTSEGVGATWDTSDDVTE
                     AEGQAFTWTLIGD"
BASE COUNT          689 a          436 c          476 g          843 t
ORIGIN      
        1 ttttaccagc aaactataga gaacgaatcg ttgttacttg aaacccgatt tcaatccaag
       61 ttgtttatta ccattagttt gctaaggtat ttcccgataa tgtcgaattc aaggttcact
      121 ttcgtgcctt tttctatggc gtgaaaattt gtgtgatgat aagtgtaagg aataattgct
      181 acctgaaaac tgttcaattt agaatttact accgttagac tcactccatt aactgttacc
      241 gatccttttt caacggtcat gtagccttgt ctggcttttt ccttgtcgaa cgggtattca
      301 aatgtgtagt accagcttcc gtcggcttct tctacctggg tacattctgc cgtttggtct
      361 acatggcctt gcacaatatg cccatcgagg cggccgttca taatcatgct ccgttcaagg
      421 tttacctcgc agccaggttc aagtagccca aggttcgact ttagcaatgt ttcttttatg
      481 gctgtaacta cgtatgtttt cttttccttg tcgattttta caacagtaag gcaaacccca
      541 ttgtgcgaaa ggctttgatc gatttttaac tcatcaataa atgaacaggt tagcgtgaaa
      601 tgatagtttt cctgatctct ctcaatcgca attactttgc cggcttcttc tactattcct
      661 gaaaacatat ttgttatttt ttaaacaaaa ataataaatt ttaagaccat ttgaaaaagc
      721 tttatactgc tgcttcacaa ttttccaatt aatgtaagtt gcttctgcat tgggttttca
      781 taagatcaga ctcatggcca gttggagacc gccaaccaat gcacataagg gtaaagccca
      841 gatgcctatg agtttgaaaa aagagttttt tgggttaaac gtagagtata gtttgggtaa
      901 aatataaacc gaaaagggca agtaaagaac gatggaagtg attactgctg gtgtataatt
      961 cattagtaat aaagttaata taatgtgtgg aatgaatgca ttcacaaaaa agacagtcgt
     1021 gataagggtt aagatatcgc gttgcaaacg ctcatttcgg gatgataaac cccagctgat
     1081 tattgcccag gcaataaggg ttatggcaat tattgctgct atcatcgggt ttgttttggt
     1141 tactgctatg ggcaagcggg agccgatttc gggcatgaaa agagcgtagc caatggcttc
     1201 ttcacaattg tggatggtaa atattattcc gctggccaac aatgctttat tgtacgataa
     1261 gtttcgcatt tttcttatag tgttttttgt gcgtctggtc tttggcgttg ataatgttgg
     1321 tcaaagaaac ttgcccattt ggatgatatt gtcgtaacaa ccatcgagtt ttcggctttt
     1381 aatacgcttt ccttcaacct caaaaccgaa ttttatataa agatttactg cggcagtatt
     1441 cgattcatat acttctagtt caactttttc cagtccatta gttttggcat gggcgagtgt
     1501 tttttcaagt aattgtgaac caagtccctg atttcggtat tctttaagta ctcccattcc
     1561 gagaactcca acatgggtaa ggccttcata acttttggga ataatatcac accagccaac
     1621 aactgtttct ttatcaatag catagtattg tgcatgattg ttctcgacaa taaatttaac
     1681 aaagtttacg gtgctttctt ccgaaaaacc agtagtcgat ccaaggtatt tgcgttcacg
     1741 ggctacaata tcaagcgatt tgcaaaacga tttggcataa tgttctgttg caaaggagat
     1801 tatcatggct aaggtgttct taaaaataaa tttgttttat acactcatca atgtgacaat
     1861 aaaagaacca aaaacttttc gaatactgtc ttatataagc atatttatga gttttgtaga
     1921 caatatttta cattttcttg ttacgcatag ccatttaatt gtaccagttt caatttatta
     1981 tgttggaatt tgttgccata tttcgtctca acaactatct ttggtttact ttttgttagt
     2041 tcttcgaaag agaattttta tatacatgca gctgtaactt ttaagtaaat ccattaatat
     2101 gaaaaaagtt tatatcaatc agggtattgc aacaagtgat ttttctttag gtaaaaaatg
     2161 tttttcttcc tttgcctcga aaaattcaat agttcacttt ttaggaagaa agaaaaaggt
     2221 cgccacaatg ttggtttttg ccttttttgc attgtttatg gtttcttgtg atcttttgaa
     2281 cgatgaaggt aagtcgttcg ataagagttt gctatatggc acctggcgat cggggacatt
     2341 atactactcg tataccagcg aaggggtggg ggcaacatgg gatacttctg atgatgttac
     2401 tgaagcagaa gggcaggcat tcacatggac gctcattggc gatc
//
LOCUS       DAOPUW010000276         2443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_45468_length_2443_cov_2.817839, whole genome shotgun sequence.
ACCESSION   DAOPUW010000276 DAOPUW010000000
VERSION     DAOPUW010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45468_length_2443_cov_2.817839"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            444..>1272
                     /locus_tag="PKJ43_05530"
     CDS             444..>1272
                     /locus_tag="PKJ43_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNZ72062.1"
                     /translation="MNEMIAVTLVYILMFPLGMLAMKLVFKKSIMKKISQLTLVVMFV
                     GGYLGFFAGRYNSYHLAWAFPVTFFVGVVIFISIRKKLADPLSNSIRSIKSLSEGNLT
                     IEIEKSNDKSEMGILTNSLCDLVASLREIISNIKANSDNLAVSSQQISSMSEQLSQSA
                     AEQASSLEELSSTLEEIMSVVKNNMELGKNTIGVTEKAEGTARRAVSGIESTIKIFNE
                     IISKVAMVNDISFQTNILSLNAAVEAARAGDAGRGFAVVAAEVRKLAEQSKILSNNVS
                     "
     assembly_gap    1273..1282
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1283..1532
                     /locus_tag="PKJ43_05535"
     CDS             <1283..1532
                     /locus_tag="PKJ43_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72063.1"
                     /translation="SQSEKEFGELMPDISKATKHVQQIVHASMEQSTGIEQIGTSVFQ
                     MNNVTQQSAAASEELASNAEELEHQAKQLKQIVAHFHL"
     gene            1562..2365
                     /locus_tag="PKJ43_05540"
     CDS             1562..2365
                     /locus_tag="PKJ43_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNZ72064.1"
                     /translation="MTQLKSKKVVITGGASGIGKEMAKIVLSKGISELVLWDYDAVKL
                     DETVVELQSWNIPVHSFVVDVSDTGQIILAARKTLDLIGKADILINNAGILIGKMFHE
                     HSHEDITREMNINALGYMHVAREFLPSMIEQRSGHICNVASAAGLLGNPRMSIYCASK
                     AAVINWADSLRIEMDQMKTNVKVTIVTPNYIHTDMIPGVKSVIPILKKEVAAKKIIRG
                     IERNKAFVRMQFIVSITPILKAILSIRMFDFFVGKLLGVYNTMNGFKKN"
BASE COUNT          761 a          417 c          523 g          732 t
ORIGIN      
        1 ctggtattct tgggcgaagg tgagaatagg caatgcaatc aatattgtta caaaaagcaa
       61 ttgtctgatt gtttgtttct tacaatatat agtaaaattt ttcatgggta agtaattttt
      121 agtttataat tggtaatagt acccgatcag gcgggggagt aacgggtgat cgtatttgca
      181 gccactaacc gggttcaaat aattaacttc ctaaagataa aaaaatttat ttaaaaacaa
      241 tatgttttat aacataaaat aataaaaaac aacaggcaca ttacagtcag tataagattt
      301 aacaattacc tgatatacac tgcttttttt tcgcacccct tcatttgttc attatttttt
      361 tgagaaataa tgtgtaaaat atgtttaatt tcttaaatgt ttttttaaat ttaatttttc
      421 ttaacaaaat aaaccaatat gttatgaatg aaatgattgc tgttacactg gtgtatattt
      481 taatgtttcc actgggaatg cttgccatga aactggtttt taagaaatcg ataatgaaaa
      541 aaatatctca gcttacattg gttgttatgt ttgttggagg atatcttggt ttttttgccg
      601 gacgatataa ttcataccat ttggcttggg catttccagt tacttttttc gtaggggtag
      661 tcattttcat ttcaatcaga aaaaagcttg ccgatccgtt aagcaattct attcgttcta
      721 ttaaatcgct atctgaaggt aatttgacaa ttgaaataga aaaaagtaac gataaaagcg
      781 aaatgggtat tctgaccaat tcattgtgcg atttggttgc cagtttgcgg gagattattt
      841 caaatattaa ggccaattcc gataatttgg cggtaagtag ccagcaaatt agttcaatgt
      901 cagaacaact gtctcaaagt gccgcagagc aggcttcaag tctcgaagaa ctttcatcaa
      961 cacttgagga gatcatgtca gtagtaaaga ataacatgga gctgggcaaa aataccattg
     1021 gtgtaaccga aaaggctgaa ggtacggctc gaagagcggt cagtggcatt gagtcgacca
     1081 taaagatatt caacgaaatt atttcaaagg ttgccatggt aaacgatatt tcgtttcaga
     1141 ccaatatcct ttctttaaat gcagctgttg aggctgcccg cgctggcgat gcagggcgcg
     1201 ggtttgctgt tgttgctgcg gaggttcgta agctggccga gcaaagcaaa atattgtcga
     1261 acaacgtgtc ttnnnnnnnn nncagtcagt cggaaaagga atttggggag ttgatgcccg
     1321 atatttcgaa agcgacaaaa catgtgcagc aaattgtgca cgccagtatg gagcaaagta
     1381 caggaattga acagattggt acttcggttt ttcagatgaa caacgtaacc cagcaatcgg
     1441 cagccgcttc cgaagaactg gcatcgaatg ccgaagagct tgaacatcag gccaaacagc
     1501 ttaaacaaat agtcgcacat tttcatttgt aatctattct gatcaattag tcacataaat
     1561 catgacacag ctaaaatcaa agaaagttgt tattaccggc ggagcatccg gaattggcaa
     1621 agaaatggcc aaaattgtat tgtcaaaagg tatttcggag cttgtccttt gggactacga
     1681 tgctgtaaaa cttgacgaaa ccgttgttga gcttcagtcg tggaatatac cggtccattc
     1741 gtttgttgtt gatgtttccg ataccgggca aattatttta gctgccagaa aaaccctcga
     1801 tcttatagga aaggccgata tcctgatcaa taatgcagga attttgatcg gaaaaatgtt
     1861 tcatgaacac agccatgaag atataacccg cgaaatgaat atcaatgccc tgggatatat
     1921 gcatgttgcc cgtgaatttt tgccttcgat gatcgaacaa cgttcgggac atatctgcaa
     1981 tgttgcttcg gcggctggtt tgctcgggaa tccacgtatg tcgatctatt gtgccagtaa
     2041 agctgctgta atcaattggg ccgacagctt gcgcattgag atggaccaaa tgaaaaccaa
     2101 cgtgaaagtt accattgtta ccccaaatta tatccataca gatatgattc ccggggtgaa
     2161 atcggtaata ccaatcctga agaaggaggt ggctgctaag aaaattatca ggggtattga
     2221 acgcaacaaa gcttttgtaa gaatgcagtt tattgttagt ataaccccga ttctgaaagc
     2281 cattttatca atacggatgt ttgatttttt tgttggtaaa ctgctggggg tttataatac
     2341 catgaacggg tttaaaaaga attgattcag cgcatacaat ttgcaatcaa tgaaccctga
     2401 tatattttaa tgggacgcgg atgcaacgga tgcaggcaac gcg
//
LOCUS       DAOPUW010000277         2428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_45825_length_2428_cov_3.512853, whole genome shotgun sequence.
ACCESSION   DAOPUW010000277 DAOPUW010000000
VERSION     DAOPUW010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45825_length_2428_cov_3.512853"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PKJ43_05545"
     CDS             <1..475
                     /locus_tag="PKJ43_05545"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72065.1"
                     /translation="ERLVVTEGEDKPIEVDPTKVRADVHRVLGQSLKLRQISAAGDNS
                     CEWELNAANNVQFDMSRFGIDFVASPRHADGILITGPISENMAEPVQLCYDATPAPKI
                     VVLAGTDAISGGIFGDSPAIDRSFLSKYPVDLYIPGNPCHPLTIINGLKDLTRKK"
     gene            588..1022
                     /locus_tag="PKJ43_05550"
     CDS             588..1022
                     /locus_tag="PKJ43_05550"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNZ72066.1"
                     /translation="MKKIFLSLFIFSCTLTLFAQQPVQPAVADTVAKAKIVFEYLNHN
                     YGQIAYGSDGTCTFKFKNEGAVPLILSNVRASCGCTTPSWTKDPVAPGQSGEIKVTYD
                     THRIGAFSKSVTVTSNAVETPSVVLRISGEVKQNTEPQTSVN"
     gene            1045..1413
                     /locus_tag="PKJ43_05555"
     CDS             1045..1413
                     /locus_tag="PKJ43_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNZ72067.1"
                     /translation="MKALYTIGLLILSNTFMTLAWYGHLKFKEYKWFESLPLIMVVLI
                     SWGIALFEYFFQVPANRIGYKGNGGPFTLFQLKVIQEAITLIVFTLFAVFAFKTETFK
                     FNHFIAFVFIILAVYFVFKE"
     gene            1535..>2428
                     /locus_tag="PKJ43_05560"
     CDS             1535..>2428
                     /locus_tag="PKJ43_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNZ72068.1"
                     /translation="MNKLTVILMLLFTSIGYYADAQQSLWNDSKLVSPQINNDNSVTF
                     RVEAPGAREVKIIGDWIPSAGQSTGGVTMKLGENGVWSYTSAPLASDLYSYLFIIDGI
                     KTNDPNNVYQIRDVASIFNVFIVKGGKGDLYSVNDVPHGTVSRCWYNSPGLSKDRRIT
                     VYTPAGYEKSKEKYPVLYLLHGMGGDEEAWIALGRTAQILDNLIAQGKARPMIVVMSN
                     GNASQQAAPGEAAAGMHKPTMMLPNTMDGTFETSFPDIISFVEQNYRVIAEKKGRAIA
                     GLSMGGFHSLHISRFYPNTFDH"
BASE COUNT          694 a          495 c          521 g          718 t
ORIGIN      
        1 tgaaaggctt gttgtaactg aaggtgaaga caagcctatt gaagtagatc ctacaaaagt
       61 gagagccgat gtgcatcgcg tgttggggca atcgctgaag ctcaggcaaa tttctgcagc
      121 tggagacaac agctgcgaat gggagctcaa tgcggccaac aatgttcaat tcgatatgag
      181 ccgttttggg atcgattttg ttgcttcgcc acgccatgcc gacgggattc tcattactgg
      241 ccccatatcg gaaaacatgg ccgaacctgt acaactgtgt tacgatgcaa cccctgctcc
      301 taaaattgta gttctggcag gaacagatgc catcagcggt ggaattttcg gtgatagtcc
      361 cgccattgac aggagttttc tgagcaaata ccccgtcgat ctgtatattc cgggcaaccc
      421 gtgccatccg ctgactatta tcaacgggtt aaaagacctg acacggaaaa aataattgat
      481 acattttgtt tttttgttgt taaaaatcag gcaggaaagc acacaacatg aaaagaatct
      541 ataattttgt cgaacttata aaacaggaaa cttaaaatat ataccaaatg aaaaaaatat
      601 tcttatcgtt atttatcttc agttgtaccc ttacattgtt tgcacaacaa ccagtacaac
      661 cagccgttgc tgatacagtg gcaaaagcta aaattgtttt tgaatacctg aaccacaatt
      721 atggtcaaat tgcgtatggt tcagatggaa cctgtacttt caagtttaag aacgaaggtg
      781 ctgtaccatt gatcctttct aatgtaaggg cttcttgcgg ctgcactact ccgagctgga
      841 ccaaagatcc tgttgcaccg ggccaaagcg gagaaattaa agtaacttac gatacgcatc
      901 gcataggcgc tttctcgaaa tcggtaaccg taacgtcaaa cgctgttgaa accccttctg
      961 tggttttgcg catttcggga gaagtgaaac aaaacacaga accacaaacc agtgttaatt
     1021 aatttgcttt tgattgatct gtaaatgaag gcattatata ctattggatt gctaattctt
     1081 tccaatacat ttatgacgct tgcctggtat ggtcatctta agtttaaaga atacaaatgg
     1141 tttgagtctc ttcctctgat tatggtagtt cttattagct gggggattgc gctgttcgag
     1201 tatttttttc aggtaccagc caaccggatt ggttataaag gcaatggcgg cccgtttacc
     1261 ctttttcagt tgaaagttat tcaggaagca attaccttaa ttgtttttac attatttgca
     1321 gtttttgcct ttaaaacaga aacatttaag ttcaatcatt ttattgcctt tgtttttatc
     1381 attctggccg tttattttgt cttcaaagaa tagaaatata cctaataaaa aaagccgggg
     1441 agatttttca cccggctttt tttatcttat tttccctctc gtgccaaaat ttctacctat
     1501 cttaatcaac caattaacga tagattagat taccatgaat aaactgactg taattttaat
     1561 gttgctgttc acatccattg gctattatgc tgatgcacag caatctttgt ggaacgattc
     1621 gaaacttgta tcgccacaaa tcaacaacga taactcggta acttttaggg ttgaagcccc
     1681 gggagcccgc gaagtaaaaa ttattggcga ttggattcct tctgcagggc aatccaccgg
     1741 gggtgttacg atgaaattgg gtgagaatgg tgtctggagc tatacttcag ctccgttagc
     1801 cagcgatttg tacagctatc tttttattat agatggcata aaaaccaacg atccaaacaa
     1861 tgtttatcaa attcgcgatg tagcttcaat tttcaatgtg tttattgtaa aaggggggaa
     1921 aggcgacctc tattcggtga acgatgtacc tcacggtacc gtttcacggt gttggtacaa
     1981 ctcgccaggc ctttcaaaag atcggcggat aaccgtttat acaccagctg gatatgaaaa
     2041 aagtaaggag aaatatccgg tgctgtactt gctgcatgga atgggtggcg atgaagaagc
     2101 ctggatcgcg cttgggcgta cagctcaaat tctcgataac ctgatcgcgc aaggaaaagc
     2161 caggccaatg attgtggtta tgagcaatgg aaatgcatcg caacaagctg ctcccggtga
     2221 ggctgcggca ggaatgcaca aacctacaat gatgctcccc aataccatgg acggaacttt
     2281 tgaaacttct tttcccgaca taatttcttt tgttgagcaa aattataggg ttattgccga
     2341 gaaaaaggga cgggctattg caggcttatc gatgggcggt tttcactccc tgcatatttc
     2401 gcgtttctat cccaatacct ttgaccat
//
LOCUS       DAOPUW010000278         2421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_46011_length_2421_cov_3.045647, whole genome shotgun sequence.
ACCESSION   DAOPUW010000278 DAOPUW010000000
VERSION     DAOPUW010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46011_length_2421_cov_3.045647"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..877)
                     /locus_tag="PKJ43_05565"
     CDS             complement(77..877)
                     /locus_tag="PKJ43_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72069.1"
                     /translation="MAKIEDQRIVIDCTCEVQLQQTIIDAVYLALKLEKEICFFGNYL
                     NNSEKEKLQQRTAFYANFVKKDIPSLPVSVLLLEGKLNNLVKDLGEKYNTILYCYSGA
                     MDNRLLEAFYRSGFPFYFSKKKTSKGNQFSKVLIPVDFRNSSKDSALWGSYLGRFCNA
                     EIKLLVASEKNTEDRDLVASNTESISRLFTQFSFPWSSDFGKSSSWGIHEEAVKNEEP
                     FDVIIFSGSLNVTLVDKITGTFEKRLVNQSEKSVLLINPQRELYLICS"
     gene            980..>2421
                     /locus_tag="PKJ43_05570"
     CDS             980..>2421
                     /locus_tag="PKJ43_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNZ72070.1"
                     /translation="MINQQLFNPQSIVVVGASNDVTKPGGKLLKNLIDSNYAGNLMVV
                     NPSSSIVQGISAMASVSEVVHADLAVLAIPAASCPESVRILAEKGVKAFIIISAGFSE
                     VSDEGAALEAELKQIVDQHEACLIGPNCIGVLTPFHASVFTKPIPRLHPCGVDFISGS
                     GATAVFIMESAIPKGLNFHSVISVGNSTQTGIEDVLEYFDETYDPATSPNVKLIYLEN
                     IKNPDKLLYHASSLVRKGCHIAAIKAGTSVAGSRAAASHTGALASPDLAVEALFRKAG
                     IVRCHGREELTTVACVFMSKPLKGRNIAIVTHAGGPAVMLADALENGGLTIPHLEDCE
                     AKTKLKEMLFAGSSVENPIDFLATGNEQQLNAIIDACENDFDNVDAMAVIYGSPGLFP
                     VRDVYKMLDKKMRECRKPIYPILPSVINVKEDLAYFLSHGNVNFPDEVLLAKALTLVY
                     QTPPPSDDNIYLDGVDIPQVRRIIEKTENG"
BASE COUNT          653 a          493 c          527 g          748 t
ORIGIN      
        1 tcgttgatga atcatttgtt ttatttggtt tttgggaaac aaattattgt tgtgtataag
       61 aataaaacgt gttgtattag ctgcatataa ggtacaattc gcgttgggga tttataagca
      121 gtaccgattt ttctgattga ttgactagcc ttttttcgaa ggttcccgta attttatcga
      181 ccaaggttac atttaggctt cctgaaaaaa ttattacatc gaatggttct tcattcttaa
      241 ccgcttcttc gtgaattccc caactgctgc ttttcccaaa atctgaagac catggaaatg
      301 aaaactgggt aaaaagccgg cttatcgatt ctgtattgct ggctactagg tcccggtctt
      361 ctgtgttttt ttcgctagca acaagtagtt taatctcggc attgcaaaag cggccaaggt
      421 aacttcccca aagggctgaa tctttgctgc tatttctaaa atcgacggga ataagcactt
      481 tactgaactg attgccttta ctcgtttttt tcttgctaaa atagaatgga aaaccagagc
      541 ggtagaatgc ttcgagcagg cggttatcca tagctccgct gtagcagtat aaaatagtgt
      601 tgtatttctc gcccaggtct tttactaaat tgttcaattt cccttcgagt aaaagtaccg
      661 aaacaggcaa cgatgggata tcttttttta caaaattggc ataaaacgct gtgcgttgct
      721 gcaacttctc cttttcgcta ttattcaaat agttcccaaa aaaacatatt tctttttcaa
      781 gttttaaggc taggtaaaca gcatcgatta ttgtttgctg aagttgtact tcgcaggtac
      841 aatcgattac tatacgttgg tcttcaattt tcgccatttc tgaaaacttt ttactgtttt
      901 gtgcgtaata tcctctctat aatgaattat gtcagtagtg actcgaccca aaaccattaa
      961 ttttgaaaaa aaatcaggta tgattaatca gcaactgttc aatccgcaga gtattgttgt
     1021 ggttggtgct tcaaacgatg taaccaaacc cggcgggaaa ttactaaaaa accttattga
     1081 ctcaaactat gcagggaact tgatggttgt aaacccatct tcctcgattg ttcagggcat
     1141 ttcagcaatg gcatcggtaa gcgaagttgt ccatgccgat ttggctgtgc tggcaattcc
     1201 agcagccagt tgccctgagt cggtgcgcat attggcggaa aaaggagtga aagctttcat
     1261 cattatttcg gctggattta gtgaggtaag tgacgaaggt gctgctcttg aggcggagtt
     1321 gaaacaaatt gtagaccagc acgaggcttg ccttattgga cccaattgca ttggggtact
     1381 taccccattc catgccagtg tatttactaa accaattcca cggttgcatc cttgtggagt
     1441 cgattttatt tcagggtcag gagctacagc cgttttcatt atggaatcgg ctatccccaa
     1501 aggattgaat tttcactcgg ttatttcggt tggaaattct actcaaacag ggattgaaga
     1561 cgtgcttgaa tattttgatg agacgtatga tccggcaact tctcccaatg ttaaactgat
     1621 ctatcttgaa aatatcaaaa atcccgataa attattgtat cacgcttcgt ctttggttcg
     1681 taaaggatgc catattgcgg ccatcaaagc tggtacatcg gttgccggaa gccgcgctgc
     1741 agcttcgcac acaggcgctt tggccagtcc cgatctggct gttgaagccc tctttcgtaa
     1801 agcaggtatc gtgaggtgtc acgggcggga agaacttaca actgtggcct gtgtctttat
     1861 gagcaaacca ttgaagggta ggaatatagc tattgttacc catgcaggag gaccggcagt
     1921 tatgctcgcc gatgctctcg aaaacggggg gctaaccatt cctcatctcg aggattgtga
     1981 ggcaaaaaca aaattgaagg aaatgctttt tgcgggctcg tcggtcgaaa acccgatcga
     2041 tttcctagct actggaaacg aacagcaatt gaatgccatt attgatgcct gtgaaaatga
     2101 ttttgacaat gtagatgcaa tggctgtcat ttatgggagt cctgggcttt tccctgtgcg
     2161 tgacgtgtac aagatgctcg ataagaaaat gcgcgaatgc cgaaaaccta tttacccaat
     2221 attgccatcg gtgatcaacg ttaaagaaga tttggcttat ttcctttccc acggaaatgt
     2281 aaattttccc gacgaagttc tgcttgccaa ggcattaacg ttggtttatc aaacccctcc
     2341 accatccgat gacaatattt acctcgatgg ggttgatatt cctcaggtcc gtcgtatcat
     2401 cgaaaaaact gaaaatggct t
//
LOCUS       DAOPUW010000279         2417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_46137_length_2417_cov_2.110923, whole genome shotgun sequence.
ACCESSION   DAOPUW010000279 DAOPUW010000000
VERSION     DAOPUW010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46137_length_2417_cov_2.110923"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PKJ43_05575"
     CDS             complement(<1..396)
                     /locus_tag="PKJ43_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72071.1"
                     /translation="MRKGLLLLLFLTITFDFAFASNPYIDSLARQKATIPNDSLRFDA
                     LKKLAIAYSDSAYDVSIMYWREALLLSKRKNNKLWIGDVHQQLGYMLHRKGELSEALE
                     EMNSALSHYMSIKNYVSIAEVYNDMGLIYK"
     gene            complement(512..1483)
                     /locus_tag="PKJ43_05580"
     CDS             complement(512..1483)
                     /locus_tag="PKJ43_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNZ72072.1"
                     /translation="MKRILTIAMMAVISLYAIAQKNPVDILIDLEFADTLEANTMDTL
                     KLTPDTKQQTSVVEGTEKTTSPDTTKIRFGKRNIVIVEKDGTTSIEIPELKESGENIE
                     IREKSKPKKFKGHWAAVEWGFNGYMDANHSINMKDDLRYLELKQGRSWNFNINFLQYS
                     LGFNTDKAGLVTGLGFEFNNYHFRNPITLTAGTPVTGADSTYILDPNKNVVKSKFSTT
                     HLTVPLLFEFQIPTGHSKHRIFFSTGVIGGVKIGSSTKVQYEGTKKGKDKTRNDFNLS
                     QFRYGLTARIGYGGFRVFANYYPTPLFEEGKGPELYPFTVGLILLKL"
     gene            complement(1552..>2417)
                     /locus_tag="PKJ43_05585"
     CDS             complement(1552..>2417)
                     /locus_tag="PKJ43_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72073.1"
                     /translation="EGLKKTFSQGMGIDDFDYLCIANIEGELAPDEENLLNELLAEIP
                     QRMSDYSLFQKAKLEPDLHLVYKPKQSLKRNTIFKAKQSTVNSFVSIAAGIALVFGLY
                     SVFSIYKQMGIHPGLTEIAEIKTSNNTLDVTYKEEHAVKSVGDSFSYTGYINQTKPKA
                     QPQTDPSSEQHYFNREQLNFSYIEPLRIEEFKDHNADPSRYALNLAFLKESNSYGNST
                     ERVAQNSQPVGNTRTIGVFELAQLGLQKLSAYTGSPIELSAEKANDGTLKTIHFESTL
                     FAFSTNVRKKE"
BASE COUNT          654 a          474 c          513 g          776 t
ORIGIN      
        1 tttgtagatt aagcccatgt cgttatatac ttcggctatg ctcacataat tcttaatact
       61 catgtagtgt gagagtgcgc tgttcatctc ctcaagcgct tcggaaagtt ccccttttcg
      121 gtgcaacata tacccaagtt gctgatgcac atcgccaatc caaagtttgt tgttcttacg
      181 tttggacagg agcaaagctt ctcgccagta cataatgctg acatcatagg cagaatcgga
      241 ataggctatg gcaagctttt tcaaggcgtc aaaccggagc gaatcgtttg gaatagttgc
      301 cttttgacgg gccaaactat ctatatacgg attcgatgca aaggcgaaat caaaggtgat
      361 tgtaaggaat aggagtagca gaagcccttt tctcattttt accgtgctga tgtgcagggc
      421 aaaaatagta aaaacaaagg tttttaagaa gggaaaggcg ggcaatattt ttattgaccg
      481 ccttataccc tatggatcaa ataaacaatg attataattt cagtaggatt aaacctacgg
      541 taaaggggta aagctctggg cccttgccct cttcaaaaag aggcgtggga taataattgg
      601 caaaaactct aaatcctccg taaccaattc tggccgttag accataccta aattgcgata
      661 agttaaaatc gtttctcgtt ttatccttgc cttttttagt accttcgtat tggacctttg
      721 tactcgatcc tattttaacc ccgccaatta ccccggtgga aaagaaaata cgatgtttac
      781 tgtgccccgt gggaatttga aattcaaaaa gaagaggaac agtaaggtgg gtggtggaaa
      841 atttactttt tacaacattc ttattgggat ctaagatata ggtgctatct gcgccggtaa
      901 ccggagttcc ggcggtgagc gttattggat tacgaaagtg gtaattgtta aattcaaacc
      961 ccagccccgt aaccaagcct gccttatctg tgttaaaccc aagactgtac tgtaaaaaat
     1021 tgatattaaa attccacgat ctgccctgtt ttaactcaag gtagcgtaaa tcatccttca
     1081 tgtttatcga atggttagcg tccatatagc cattgaagcc ccattcaact gcagcccaat
     1141 gccctttaaa ttttttgggc ttactctttt ctcggatctc tatattctcg cccgattctt
     1201 taagttcagg gatttcaatg gaagtggtgc catccttttc aacaatcaca atattgcgtt
     1261 tcccaaaccg aattttcgtc gtatcaggag atgttgtttt ttctgttccc tccacaacag
     1321 aggtctgttg tttggtatca ggtgtcagct tgagggtatc cattgtattg gcctccagcg
     1381 tgtcggcaaa ttccaggtca ataagaatat ccacggggtt cttttgtgcg atggcgtaaa
     1441 ggctaattac agccatcatt gctattgtca gaattctttt catgataaag tgttttaaaa
     1501 aaattgtttg ccttatgacg ggttgataac gcgaaaaatt acaggagctt tttattcttt
     1561 tttcctaacg tttgttgaaa aggcgaatag ggttgattca aaatgtatgg tttttagtgt
     1621 accatcattt gctttttctg cacttagctc aatgggcgaa ccggtgtagg ctgataattt
     1681 ttgtaaccca agctgtgcca attcaaaaac tccgatagta cgagtgtttc cgactggctg
     1741 actgttttgc gctactcttt cagtgctatt cccataggag tttgattctt ttaggaaggc
     1801 aagattcaag gcgtatctac tggggtcggc attgtgatcc ttgaattcct ctatccggag
     1861 gggttctatg tatgaaaaat taagctgctc cctattgaaa taatgctgtt ccgaagatgg
     1921 atctgtttgt ggttgtgcct tgggctttgt ttgattaatg tatcctgtgt aagaaaaact
     1981 gtcgccaact gatttaacag catgttcttc tttataggtt acatccaatg tgttgtttga
     2041 agtttttatt tcagcaattt cagtcaatcc gggatgtatg cccatctgtt tgtaaatgga
     2101 aaaaactgag taaaggccga ataccaaggc aataccggct gcaatactta caaagctgtt
     2161 aacggttgat tgttttgctt taaaaatagt attccgtttt aacgattgct taggtttgta
     2221 aacaagatgc aaatccggtt ctaatttggc tttctggaaa agagagtaat ccgacattct
     2281 ctggggaatc tcggcaagaa gctcattgag caggttttct tcatcgggag caagttctcc
     2341 ttcgatgttg gcaatgcaca ggtagtcaaa atcatcaatt cccataccct gagagaaggt
     2401 tttttttaaa ccctcct
//
LOCUS       DAOPUW010000280         2413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_46248_length_2413_cov_3.588634, whole genome shotgun sequence.
ACCESSION   DAOPUW010000280 DAOPUW010000000
VERSION     DAOPUW010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46248_length_2413_cov_3.588634"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PKJ43_05590"
     CDS             <1..201
                     /locus_tag="PKJ43_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72074.1"
                     /translation="TLTPDLDRDLNRFREEISLLLKNEIIARYFYQKGSTQATLETDD
                     AVKKAKEILANSSDYKKTLLPK"
     gene            427..1353
                     /gene="mdh"
                     /locus_tag="PKJ43_05595"
     CDS             427..1353
                     /gene="mdh"
                     /locus_tag="PKJ43_05595"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNZ72075.1"
                     /translation="MKVTVVGAGAVGASCAEYIAIKDFADEIVLIDIKEGFAEGKAMD
                     LMQTASLNGFDSTITGTTNDYSKTANSDVAVITSGIPRKPGMTREELIDINAGIVKTV
                     AENLIKYSPNVILIVVSNPMDTMAYLAHKATGLPKNRIIGMGGALDSARFKFRLAEAL
                     GCPQSDVDGIVIGGHSDTGMVPLIEKAVRNSVPVSEFLSEEKMAEVVEATKVGGATLT
                     KLLGTSAWYAPGAAVSELVKAIALDSKKLFPCSALLEGEYGLTDISLGVPCILGKNGI
                     ESIVEIALSDAEKEKLVASAAGVREVNNLLTV"
     gene            complement(1471..>2413)
                     /locus_tag="PKJ43_05600"
     CDS             complement(1471..>2413)
                     /locus_tag="PKJ43_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNZ72076.1"
                     /translation="VCPAENFLVVTNAEYKDTVLEQLPALKPEQILSEPLRRNTAPCI
                     AYACHKIKCREENATIIVAPSDHLISREDEFIDQIKNGINFVTQNDALLTLGITPHRP
                     ETGYGYIQVSDKVDFMDITNLYRVKTFTEKPDLKMARVFLDSGEFYWNSGIFIWSLKS
                     IISALEKHLPEVNDLFSKGNKYYFTPSEKAFLSKTYSECPNISIDYGLMEKAKNVFVL
                     CADFGWSDLGTWGSLWENSTKDTNDNALNGKNIQLYNTKECIVNVSGDKLTVINGLEG
                     FIVVEADNTLMICKKEDEQQIRQIVNDIKMSKGDKYI"
BASE COUNT          668 a          434 c          548 g          763 t
ORIGIN      
        1 acattgactc ctgatctcga tcgcgatttg aaccgtttca gggaagagat ttcgttgttg
       61 cttaagaatg aaattattgc acgttatttc tatcaaaaag ggtcgacaca agccacactt
      121 gaaactgacg atgcagtgaa gaaggcaaaa gagatattgg caaacagctc agattataaa
      181 aaaacacttc tgccgaagta agccttttct gtaaaaaata tgtttaactc agtgacagtt
      241 cgctggatct gagagatggg gaaacaacct gttcggttta tttttggacg aaggttgttt
      301 tccttttatc taattgttaa cttgtgaggt aagtgcaggt tggctggagt tggatatcca
      361 tttcgagatt atttttgttg ctgacagaaa tcaagaatta ttaaaatttt taaatacaga
      421 atcaatatga aagtaacagt tgttggagca ggcgccgttg gtgctagttg tgcagagtat
      481 atcgctatta aagactttgc tgatgagatt gtattgatcg acatcaaaga aggttttgct
      541 gaaggaaaag caatggattt gatgcagact gcatcgctga acggtttcga ttctactatt
      601 acaggaacga caaacgatta ttcaaagaca gctaacagtg atgttgctgt tattaccagt
      661 ggtattcccc gtaaaccagg gatgacccgt gaagaattaa tcgatatcaa tgcagggatc
      721 gttaaaacgg tagccgaaaa cctgattaaa tattcgccta acgtgatatt aatcgtggtg
      781 agcaacccta tggataccat ggcttatttg gctcacaaag caacgggtct tcctaaaaac
      841 cgtatcatcg ggatgggtgg agcgttagac agtgcacgtt ttaaattccg ccttgccgaa
      901 gcgctgggat gcccgcagtc tgatgttgat ggaatcgtta taggcggtca ctctgataca
      961 ggtatggtgc ctctgattga aaaggcagta cgcaatagtg tgcctgtttc agaattcctt
     1021 tctgaagaaa aaatggctga ggtggttgaa gctacaaaag ttggtggcgc tacattaacc
     1081 aaattgctcg gaaccagtgc atggtatgca ccaggagctg ctgtatccga attggtaaaa
     1141 gctattgctc tcgattctaa gaaattattc ccttgttctg cccttttaga aggagaatac
     1201 gggttgactg acatttcgct tggtgtacct tgtattttgg gtaaaaacgg aattgagtcg
     1261 atcgttgaga ttgcactttc tgatgctgag aaagaaaaat tggttgcaag tgccgcaggt
     1321 gttagagaag taaacaattt gttgactgta taatatttcg gtttatttta tataaaaagc
     1381 catcgctaga gataacgatg gctttttagt gtgaatatct ttcgtttttg tgctaaaacc
     1441 tgcacagata ttattcctgt ttgaaatccg ttagatgtat ttgtcgcctt tcgacatttt
     1501 tatgtcgttc acgatctggc gtatttgctg ctcatcttct tttttacaga tcatcagggt
     1561 gttgtcggct tcaactacta taaatccctc aaggccatta atcacagtga gtttatctcc
     1621 cgaaacattt actatgcatt ctttggtgtt gtaaagttgt atgtttttgc cattcagtgc
     1681 gttgtcatta gtgtctttgg tcgagttttc ccaaagtgaa ccccaggtcc ccaggtcgct
     1741 ccatccaaaa tcggcacaaa gcacaaaaac attttttgct ttttccatca gtccgtaatc
     1801 gattgatata tttgggcact cggagtaggt tttactgaga aatgcttttt cactaggtgt
     1861 gaagtagtat ttattccctt tggaaaacaa gtcattaact tcaggaagat gcttctcaag
     1921 agcagaaatt atcgatttga gcgaccagat aaatatccct gaattccaat aaaattctcc
     1981 actgtcgagg aaaacacggg ccattttaag atctggtttt tcggtgaatg tttttaccct
     2041 gtaaagattt gtaatgtcca tgaaatcaac tttgtcgctt acctgtatat acccatagcc
     2101 ggtctcgggg cgatgggggg taatgcctaa ggtgagtagg gcatcattct gggttacaaa
     2161 gtttatccca ttttttatct ggtcaataaa ttcatcttcg cgggaaatga ggtgatccga
     2221 aggtgcaaca ataatcgtag cattttcttc gcggcatttt attttatgac aggcgtaagc
     2281 aatacatggt gcagtgttcc gtcgcagggg ttccgagagt atctgttctg gttttagtgc
     2341 tggaagttgc tctaatacag tgtctttata ttcggcattg gttaccacca gaaagttttc
     2401 ggcaggacaa act
//
LOCUS       DAOPUW010000281         2405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_46511_length_2405_cov_2.907660, whole genome shotgun sequence.
ACCESSION   DAOPUW010000281 DAOPUW010000000
VERSION     DAOPUW010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46511_length_2405_cov_2.907660"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PKJ43_05605"
     CDS             complement(<1..230)
                     /locus_tag="PKJ43_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HNZ72077.1"
                     /translation="MDTEYIETPKIGDILKEEFLAPLGITPYRLSKEIHVSTSSVLDL
                     VHNKRKITVDMALRLSKFFGTSYKFWLNLQNEL"
     gene            complement(241..522)
                     /locus_tag="PKJ43_05610"
     CDS             complement(241..522)
                     /locus_tag="PKJ43_05610"
                     /inference="COORDINATES: protein motif:HMM:NF016875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNZ72078.1"
                     /translation="MILSFGDKDTESIWNGIRVKNMPLEIQNVGRRKLRMLNNAHSIN
                     DLRQPPANHLEALKGNKKGKHSIRINDQWRILFRFVNSHASEVTITDYH"
     gene            739..2226
                     /locus_tag="PKJ43_05615"
     CDS             739..2226
                     /locus_tag="PKJ43_05615"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNZ72079.1"
                     /translation="MTQKKIVIIGSGVAGLSAGIYAAMNGFDSQIIEMHNVAGGQCTA
                     WDRKKYRFDYCLHWLVGTSHGAFHEIWKETNVLNSQTEIINHEIHSRILDKDGNEFII
                     YSDIDRWEKYLLEYAPEDEASIRKMCNDMRKSALLEPFATPPELRSPIEYITILPKML
                     PLFNVVRKFGRLTCEDYFQKLNFKNERIKSVLYNMYGGRNFSALAFIFMLGWFHQKNA
                     GYIKGGSYPFAQRMVEKFEQLGGKITYKKRVEKIIVENNVAKGVILTDGTKIDAGYVI
                     SAADGYSTIYKMLGGKYVSKEIDHAYKNWELFTPLVQVSFGINKLVESESPIIIVHNK
                     DLSIGRTKLDNGFSVMNYAFDSTMAPEGKTAIVMRYESPWKLWENLDGEAYEKEKQQI
                     EKDAIEKLEMVYPGISEFIEVIDVATPKTDVRYTGVKEGAYEGFMPTKDNMMKTINMT
                     LPKLQNFYMAGQWLSPGGGLPPAAQTGKFSVMLICKKEKQKFVVK"
BASE COUNT          749 a          480 c          458 g          718 t
ORIGIN      
        1 agttcattct gcaaattgag ccagaattta taagatgtac cgaagaattt cgaaagcctt
       61 aaggccatgt caaccgttat ttttcgttta ttatgtacca gatcaagaac ggaactggtt
      121 gatacatgta tttcttttga taaccgatag ggtgtaatac ctaagggtgc taagaattcc
      181 tctttaagaa tgtcgcctat tttgggtgtt tcaatatatt cagtatccat tatttcctcc
      241 ttaatggtaa tcggtaattg taacttcaga tgcatgtgaa ttcacaaatc taaaaaggat
      301 tcgccattgg tcgtttattc ttatactgtg ctttcctttt ttatttcctt tcaatgcctc
      361 taaatgattt gccggaggct gccttaaatc atttattgaa tgagcattat ttaacatcct
      421 caatttacga cgccccacat tttgtatttc aagcggcata tttttaaccc tgattccatt
      481 ccaaattgac tcagtgtcct tatctccaaa agataaaatc ataattacgg caatcgttac
      541 ttacgacaaa ggtaagtatt tttctacaca aattacttta atgtcttaat atctttcaat
      601 taccattttt accgttcatt ttcccctatt cgtcccgatt ttttccggca ttagtacatt
      661 ctccaaacga tcaaaataca ttataactta cgtttgccga aaattttatt atagctcgaa
      721 aaccaaaaaa ttcaaccaat gactcaaaag aaaatcgtaa tcataggcag cggagttgcc
      781 ggcttgtcgg caggaattta tgctgccatg aatggcttcg attcccaaat tattgaaatg
      841 cacaacgtag ctggcggcca atgcaccgcc tgggacagga aaaagtatcg tttcgactac
      901 tgccttcact ggctggtggg tacatcgcac ggggcgttcc acgaaatctg gaaagagacc
      961 aacgtactga acagccaaac cgaaatcatc aaccacgaaa tccattcgcg cattttggat
     1021 aaagacggaa acgagttcat tatctactcc gacatcgacc gatgggaaaa atacttgctt
     1081 gaatatgccc ctgaagacga ggcttcgatc cgcaaaatgt gcaatgatat gcgcaaaagt
     1141 gccttactag agccatttgc aactcctcct gaattgagaa gccctatcga atacatcacc
     1201 atcctgccca aaatgttgcc attgtttaat gttgttcgca aattcggacg gctgacctgt
     1261 gaagattatt tccaaaagtt gaatttcaaa aatgaacgga taaaatctgt tctttacaac
     1321 atgtatggcg gacggaattt ttcagcattg gcctttattt ttatgctcgg ctggttccac
     1381 cagaaaaatg ctggatacat caagggtggc tcctaccctt ttgcccagcg aatggttgag
     1441 aaatttgagc aacttggcgg aaaaatcacc tataaaaaac gggttgaaaa aatcattgtt
     1501 gaaaataacg tggccaaagg ggtaatcctt accgatggca caaaaatcga tgccggttat
     1561 gtaatcagcg ctgccgatgg gtattctacc atctataaaa tgctgggcgg aaagtatgtt
     1621 tcgaaagaga ttgatcatgc ctataaaaat tgggaactgt ttaccccact ggtacaagtt
     1681 tcgtttggta tcaacaagct tgttgaatct gaatctccta ttataattgt ccacaataaa
     1741 gatttaagca ttggccggac caaacttgat aacggttttt cggtcatgaa ttatgctttt
     1801 gattcaacca tggcacctga agggaaaacc gctatcgtaa tgcgatatga aagcccttgg
     1861 aaactctggg aaaacctcga tggcgaagcg tacgaaaaag aaaaacagca gattgaaaaa
     1921 gatgccatcg agaaacttga aatggtttat ccaggcatat ccgaatttat tgaagtgatt
     1981 gatgtagcca caccaaaaac cgatgttcga tacactgggg tgaaagaagg cgcatacgaa
     2041 ggttttatgc caacgaaaga caacatgatg aagaccataa atatgactct tccaaaactt
     2101 cagaatttct acatggccgg gcaatggctt tcgccggggg gaggactgcc tcccgctgca
     2161 caaaccggaa aattttcggt gatgttgatt tgcaaaaagg agaaacagaa gtttgtggta
     2221 aaataaacta atgacagttt tatttcttta atctaagtta tttaggaact tgatgttgaa
     2281 aaataatctt tgtggtgtta aggacttctt ttgaattcta aatatcacca tgccaccatc
     2341 ttaaataaca tatcattact gtttaattaa ttttttcgaa aatactgcat tatctgaata
     2401 tcctt
//
LOCUS       DAOPUW010000282         2403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_46568_length_2403_cov_4.183560, whole genome shotgun sequence.
ACCESSION   DAOPUW010000282 DAOPUW010000000
VERSION     DAOPUW010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46568_length_2403_cov_4.183560"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..350)
                     /gene="rpmH"
                     /locus_tag="PKJ43_05620"
     CDS             complement(192..350)
                     /gene="rpmH"
                     /locus_tag="PKJ43_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNZ72080.1"
                     /translation="MKRTFQPSTRKRKNKHGFRERMATANGRKVLASRRAKGRKKLTV
                     SDEPRHKR"
     gene            complement(556..1209)
                     /gene="rsmG"
                     /locus_tag="PKJ43_05625"
     CDS             complement(556..1209)
                     /gene="rsmG"
                     /locus_tag="PKJ43_05625"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNZ72081.1"
                     /translation="MPAVETYTYLTVDVILKYFPALQNSQIEQFKKIEGLYAEWNSKI
                     NVISRQDINELYLRHVLHSLAIAKLVRFTNGTKIIDVGCGGGFPGIPLAIMFPETHFT
                     LVDSIGKKIKVVNEVANALGLKNITALHERAENLPEKFDFIVSRAVTAFPDFVKMTRK
                     LISEKHSNAIPNGIIYLKGGDFSEEIASFKKTIEITEIPTYFDEPFFETKKIIYLPL"
     gene            1367..>2403
                     /gene="tgt"
                     /locus_tag="PKJ43_05630"
     CDS             1367..>2403
                     /gene="tgt"
                     /locus_tag="PKJ43_05630"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNZ72082.1"
                     /translation="MEYKLLHQCSDSKARAGLLTTDHGQIETPIFMPVGTAGSVKGIH
                     ARELDEDIKAQIILGNTYHLYLRPGTDIIEGAGGLHKFNGWHKPILTDSGGFQVFSLG
                     DIRKITEEGVRFQSHIDGSYHIFSPENSISIQRSIGADIIMAFDECTPGDADYNYAKK
                     SLEMTQRWLDRCMNQFSKTTPKYGYEQTLFPIVQGCVYPDLRQSAARFVTNYEADGYA
                     IGGLAVGEKEDDMYRMIEVVNDVLPENKPRYLMGVGTPVNLLEAIDRGVDMFDCVMPT
                     RNGRNGMLFTQKGVINIRNQKWEKDFTPVDPDGTSYVDHAYSRAYLRHLIISKEMLGA
                     QIASIHNLAFYL"
BASE COUNT          685 a          477 c          493 g          748 t
ORIGIN      
        1 atttttgtac ctgagttctt tttgtcggtt ttttatcggt tggtactttt ttgggtattg
       61 attctgatga tatattttag caaaaggcaa acgagtaacg tcagacttag agctagttgt
      121 tataaacaca aaaaggcaaa gaaaccgaat tcctcacctt gtgtccagat taaaaaaacc
      181 gtagagaacg attacctctt gtgacggggt tcgtcagata cagttaattt ttttcttcct
      241 tttgccctgc gcgaagccaa tactttacga ccatttgcag ttgccattct ttcacggaaa
      301 ccgtgtttgt tttttctctt tctggtcgaa ggctgaaatg tccttttcat cggtatatat
      361 ctttaattat tatcaatttt tatttttcaa tgaaaatggt tcgtcaaacg aagccatatt
      421 cctatcgaaa atcggactgc aaaaataatt gaatttttat cggattaaaa ttttttcaag
      481 ttttttttaa caactttaac ctgttttgcc aagtgcctgt atttaaggcg ataatattaa
      541 gcctttcagc gattgttaca acgggaggta aattattttt ttggtttcaa aaaaaggttc
      601 atcaaaataa gttggaattt cggttatctc aatcgttttt ttaaatgatg caatttcttc
      661 tgaaaaatca ccccctttta agtagattat tccattggga atggcattac tatgcttctc
      721 gcttatcagc ttgcgggtca tttttacaaa atcggggaat gcggtaactg ccctactcac
      781 gataaaatcg aatttttcgg gcaggttctc agcgcgctca tgaagtgctg tgatgttttt
      841 caagcctaag gcattggcta cttcatttac cacttttatt tttttcccta ttgaatcgac
      901 aagggtaaag tgggtttcgg gaaacataat ggctagcgga atgcccggga aacctccgcc
      961 gcagccaaca tcgataattt tagtgccgtt ggtgaagcga acaagtttgg caatggccag
     1021 cgaatgaagt acatgccgaa ggtacagctc gttaatatcc tgccttgaaa ttacattgat
     1081 tttcgaattc cattcagcat atagcccttc aattttctta aattgttcaa tctgtgaatt
     1141 ttgcagggct ggaaaatatt tcaggattac atccactgtc aaataagtat acgtttcaac
     1201 tgccggcaaa gctaattctt ttgtactttt tttgaaatac cggccgaaag aattacggtt
     1261 caagggtaca gcaatttgtt aactttccta aaagatgcta atgaatcccg acgggtgacc
     1321 tgttcaatgt aattattttt aattttgtgc gttttacaag cattatatgg aatacaaact
     1381 tttacatcaa tgttcggata gcaaggcaag ggcaggactt ttaaccactg accacgggca
     1441 aatagaaacg cctattttta tgccggtagg aacagccgga tcggttaagg gtattcatgc
     1501 ccgggaactt gacgaagata taaaggcaca aattattctt gggaatacct accatttata
     1561 tttacggcct ggaacagata ttattgaggg cgctggaggc cttcataaat tcaatggatg
     1621 gcacaaacct attcttaccg acagcggtgg atttcaggtg ttttcattag gcgacatacg
     1681 gaaaattacc gaagaagggg ttcgttttca atcgcatatc gatggatcgt accatatttt
     1741 ctcgcccgaa aattcaatct caatacaacg ctcaattgga gccgacatta tcatggcttt
     1801 tgatgagtgc accccaggtg atgccgatta taattatgca aagaagtcgc tcgagatgac
     1861 tcagcgttgg ctcgaccgct gtatgaacca attctcgaaa acgactccca aatatggata
     1921 tgagcaaacc cttttcccaa ttgtacaagg ttgtgtttac cccgatttac gccagagtgc
     1981 agcccgtttt gttaccaact acgaagccga tggctatgcc attgggggcc tggcggtagg
     2041 agaaaaagag gacgatatgt accggatgat tgaagtggta aatgacgtat tgcccgaaaa
     2101 caaacccaga taccttatgg gagttggaac cccggtcaac cttctcgaag ctatcgatcg
     2161 cggagtagat atgttcgatt gtgtgatgcc tacccgtaat ggccgtaatg gaatgctttt
     2221 tacgcaaaag ggggtcataa atatccgaaa ccaaaaatgg gaaaaagact tcaccccagt
     2281 cgaccctgat ggaacatctt atgtagatca cgcatatagc cgtgcatatc tcaggcacct
     2341 tattatttca aaagaaatgc ttggtgccca aattgccagc attcacaacc tggcttttta
     2401 cct
//
LOCUS       DAOPUW010000283         2401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_46629_length_2401_cov_3.197357, whole genome shotgun sequence.
ACCESSION   DAOPUW010000283 DAOPUW010000000
VERSION     DAOPUW010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46629_length_2401_cov_3.197357"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..333
                     /gene="rpsT"
                     /locus_tag="PKJ43_05635"
     CDS             82..333
                     /gene="rpsT"
                     /locus_tag="PKJ43_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNZ72083.1"
                     /translation="MANHKSSEKRIRQTAVKKVLNKYYAKTMRNAVKKLRGTTAKADA
                     EAMYPKVASLIDRLAKKDVIHDNKASNLKSKLALHVNKL"
     gene            422..1147
                     /gene="radC"
                     /locus_tag="PKJ43_05640"
     CDS             422..1147
                     /gene="radC"
                     /locus_tag="PKJ43_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNZ72084.1"
                     /translation="MKNIGYFGFMAEYEKLSIKMWAVDDRPREKMLKHGFGALSNAEL
                     IAILIGSGNTNETAVELSRRILTEYKNNLDQLGKTSVEKLKNFNGIGEAKAINILAAL
                     ELGRRRQLSEPGEVRRINSSQDAFQSVSLELSDLGHEEFWVLFLDRSNKIIDKIRVSQ
                     GGVSGTVFDVRIILKQAIDKLASSLILFHNHPSGNLTASSNDMDITKKACDAAKLFDI
                     KVLDHIIVAGKKYLSMADEGMLP"
     gene            1181..>2401
                     /locus_tag="PKJ43_05645"
     CDS             1181..>2401
                     /locus_tag="PKJ43_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNZ72085.1"
                     /translation="MIHIYSAELTNRMDYIFRLIFESVLGEQVDFYQNVKAFSTKTGV
                     KINYSRNSNIGGLTLQPHPLLFEKDIKPQECNVVEWKSMPVFFETDGSFLPFDIFAAS
                     FYLVSRYEEYLPGKRDRHNRFLSRNSLASQHHFLEKPLINCWAWTMANIIEQQYNECN
                     FTKSKFRYIPTFDIDNAWAFKHKGLVRNTASSIKDLLSGRFKSIGNRFSVLLASGKDP
                     YDTYDYISSLVKANHFQPVIFVLINNKGQHDRSLSHKNKSFRRLIRSLSKWSEVGIHP
                     SYSSEKNEKLLSKEIKRLDEILNKETRSSRQHYLKIMFPKTYRRLLDKGIDTDYSLGY
                     ASRPGFRASICTPFHFFDLTENETTSLTIIPFQVMDGTLLRYRNLSPNEATKKIATLM
                     NEVAVVGGTFVSLWH"
BASE COUNT          796 a          507 c          477 g          621 t
ORIGIN      
        1 taagggttag gattcaagat tttcattctt gccatagggg ttcgaatccc ctacggacta
       61 cttttaaaaa tatagctaaa aatggcaaat cacaaatcgt cagaaaagag aatacggcaa
      121 actgctgtca agaaagtact caataagtat tatgccaaaa caatgcgtaa tgcagtgaaa
      181 aaattgcgcg gcacaacagc aaaagccgat gcagaagcaa tgtacccaaa agtagcatca
      241 cttatcgacc gtttggcaaa gaaagatgtt attcacgata ataaggcttc aaaccttaaa
      301 tcgaaacttg cgttacacgt aaacaagttg tagaagaaat ttttaccaat catacgaagc
      361 ctcacctttc ggttgaggct ttttttgttt gccccatgct ttaaaaaaaa ttcttgcgtt
      421 tttgaaaaac atcgggtatt ttggctttat ggctgaatat gaaaaattat cgataaaaat
      481 gtgggccgta gatgacaggc cccgtgaaaa aatgctcaaa catggctttg gagcactaag
      541 taatgccgaa ctaatagcaa ttttaatagg ctcgggcaat acgaatgaaa ctgcagtaga
      601 actctcgcgc cggattctta ccgaatacaa gaacaatctc gatcagcttg gaaaaacctc
      661 ggtcgaaaaa ctcaaaaact tcaatggaat tggcgaagca aaagccatca atatattggc
      721 agcgctagag ctgggacgtc gaaggcaact atcagaaccc ggtgaagtgc gcagaataaa
      781 ttcaagtcag gatgcctttc agtctgtttc acttgaactt tcggatttag gccatgaaga
      841 attttgggtg ctttttctcg accgctccaa taaaataatc gataaaatta gagttagtca
      901 gggcggagta agcggaacgg tattcgatgt gcggattata ctaaaacaag ccattgacaa
      961 actggccagt tcactcatcc tttttcacaa ccacccaagc ggcaatctca cggccagttc
     1021 aaacgacatg gatatcacaa aaaaagcatg cgatgcagca aaactcttcg atataaaagt
     1081 gctcgaccac attattgttg ccggcaaaaa atacttgagc atggccgatg aaggaatgtt
     1141 accataattt tttgtaattt tatcgcatta atcgaaagct atgatccaca tctattctgc
     1201 agaacttacc aatcggatgg attatatttt ccgattgata ttcgagtcgg ttcttggcga
     1261 acaagttgat ttttatcaaa atgtgaaagc attttccact aaaaccgggg tgaaaatcaa
     1321 ttactcacgc aacagcaaca ttggaggctt aacacttcag ccccatccgc tacttttcga
     1381 aaaagatatt aagccacaag aatgcaatgt ggttgaatgg aaatcgatgc cggttttttt
     1441 tgaaaccgac ggatcatttt tgcccttcga tatttttgcc gccagttttt atctagtaag
     1501 ccgatacgaa gaatacctgc ctggaaaaag agacaggcat aacaggtttc tttcgaggaa
     1561 cagtttggca agccagcacc actttcttga aaaaccattg atcaattgct gggcctggac
     1621 gatggccaac atcattgaac aacaatacaa cgaatgtaat ttcacaaaaa gcaaattccg
     1681 ctacatcccg actttcgaca ttgacaatgc atgggctttc aaacacaaag gattggtacg
     1741 caacacggcc tcatcaatta aagatttact ttcaggaagg tttaaatcaa taggaaatag
     1801 gttctctgtt ctccttgcct cgggcaaaga cccttatgat acatacgatt acataagtag
     1861 tttggttaag gcaaatcatt ttcaaccggt cattttcgtc ctaatcaaca acaagggtca
     1921 gcacgaccgc agcttatcgc ataagaataa atcgttcaga cgattaatac gttcactttc
     1981 gaaatggagc gaagttggca tccacccgtc atatagttca gaaaaaaatg aaaaattact
     2041 gtctaaagaa attaaacggc tggatgaaat tctaaacaaa gaaacaagat cgagccgcca
     2101 gcactatctc aaaatcatgt ttccaaaaac ataccggcgg ctcctcgaca agggaataga
     2161 caccgactac agtctgggat atgccagcag gccaggcttc agggcaagta tttgtacccc
     2221 gtttcacttc ttcgatttga ctgaaaacga aacaacatcg ctcaccataa ttccatttca
     2281 ggtaatggat ggcacattgc tccgttaccg caacctgtct ccaaacgaag cgactaagaa
     2341 aattgcaacg cttatgaatg aagttgccgt agttgggggc acttttgtaa gcctgtggca
     2401 c
//
LOCUS       DAOPUW010000284         2387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_46988_length_2387_cov_2.765866, whole genome shotgun sequence.
ACCESSION   DAOPUW010000284 DAOPUW010000000
VERSION     DAOPUW010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46988_length_2387_cov_2.765866"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1021)
                     /locus_tag="PKJ43_05650"
     CDS             complement(89..1021)
                     /locus_tag="PKJ43_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HNZ72086.1"
                     /translation="MNLLNFNEIYPDELSCKLKFKEIRDKEGIVCAHCGCKDHYWKSD
                     KWQYECKKCGFRTTLRSGTVMHGSKLPFRYWFIAMHLLTSTKKSFSAKEVQRQLSHKR
                     YEPIWGMLHKLRQAMGKRDGQYQLSGQIELDEGFFSTEIADEEKNKPLKRGRGSQKKT
                     KVLVMAESKAVEGETKKNGKPRRVRFIKMQVIDDLKAATITPVVEKEVSPTSQLTTDD
                     STSYVNLKDVVEKHEPQVVPKEKVGQMLPWVHIAISNAKRMLLDIHHDIKPDHLQNYL
                     DEFCYNFNRRYFGEALFDRLLIASASNKNEFRYR"
     gene            1209..1769
                     /locus_tag="PKJ43_05655"
     CDS             1209..1769
                     /locus_tag="PKJ43_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72087.1"
                     /translation="MKKISIVKIIISLLCFVLLLQCCKLDKDFCRLSSFDKEFTLEEY
                     ESLEYLENGTTKINVKIESKQNDSWNKNGGWFYTGDGGYESIHSNFYVSDSSRFIEYA
                     IVACRDTCWIAIWKDRYSQYTSAFYFNKESLTGIKRTIQSKEYENCYVFSDTIDIKEL
                     IYVKDYGVVKLELLNGYSIELIRKIK"
     gene            1766..>2387
                     /locus_tag="PKJ43_05660"
     CDS             1766..>2387
                     /locus_tag="PKJ43_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72088.1"
                     /translation="MKHIISLLLVLATLQLVAQTTLNFRTTMFNNLTTSKLSTNVLAN
                     KAGMPDSIFGFNGSSTCNASTFASWQYAYQSLYVGFVGTPSYPTPETILAQVPDSPTS
                     EIKINGLYYKYNHLADNAVSSGLIHLTDTLFYDGPNSGNPYLEKTCVAVSPMYPAEVP
                     FNVIFRGGAIYGNLTANVASASIDFGNGSGYVSFVANNLYDISYSSE"
BASE COUNT          658 a          529 c          477 g          723 t
ORIGIN      
        1 tggaacctgg catgtacctc tataccctta ttgccgatgg caaagaggtg gatactaaaa
       61 agatgatttt aacgaaaaat gaatatcctc atctatacct aaattcattt ttatttgatg
      121 ctgaagcgat taaaagcctg tcaaacaatg cttccccaaa ataccgccgg ttgaagttgt
      181 agcaaaattc atcgaggtaa ttttgaaggt ggtccggttt tatgtcatgg tgtatgtcga
      241 gcagcatccg cttagcattg ctgatggcga tatgcaccca aggcagcatt tgcccgacct
      301 tttctttggg gacaacttgc ggttcatgtt tttcaactac atctttcaag ttgacgtacg
      361 atgtcgaatc gtcggttgtt agctgtgaag tgggcgacac ttctttttca accacaggtg
      421 ttatggtagc agctttcagg tcgtctatca cctgcatctt gatgaagcgg actctccggg
      481 gcttgccgtt cttcttggtt tccccttcaa cggccttgct ttctgccatc accagcactt
      541 tggtcttctt ctggctgccc ctgccccgtt ttagcggctt gttcttttct tcatcggcta
      601 tttctgtgga gaaaaagccc tcgtcaagtt ctatctgccc tgataattgg tattgcccgt
      661 cccttttgcc catcgcttgc ctcagtttgt ggagcatgcc ccaaatgggt tcgtaccgct
      721 tgtgcgagag ttgccgttgg acttctttgg ccgaaaaact ctttttggta gaggtcagaa
      781 ggtgcattgc tataaaccaa taacgaaagg gcaacttgga gccatgcatc acagtgccac
      841 tgcgcagtgt cgtgcggaaa ccgcacttct tgcattcgta ttgccacttg tcgcttttcc
      901 agtagtgatc cttgcaacca cagtgcgcgc atacaattcc ttccttgtcc cttatttcct
      961 tgaacttgag tttgcagctc agttcatctg gatatatctc attgaaattt aataggttca
     1021 tatctttctc gcttatatcc caaagatact gaaaaaaatc atattttagg tataatttcg
     1081 gatattcatt taacgaaata aaacacaagt gcatcacaac agtgcacaaa tgccataccc
     1141 catttgaggg tttagtggca actttacttg ccatgtttct atctatgaac taatgtaaaa
     1201 atggaacgat gaagaaaatt agtattgtta aaatcataat ctcattgctt tgctttgtac
     1261 tattactaca atgctgcaaa ttagataagg acttctgcag gctttcttct ttcgataaag
     1321 aatttacttt ggaagagtac gaaagtttgg aatatcttga aaatgggaca acaaaaatca
     1381 acgtaaaaat cgaatctaaa caaaatgatt catggaataa aaatgggggt tggttttaca
     1441 cgggtgacgg tggctacgaa tcaattcact caaactttta tgtttcagac agctctcgtt
     1501 tcatcgaata tgctattgtt gcatgccggg atacatgctg gattgccatt tggaaagatc
     1561 gttattcaca gtatacctca gcattttatt tcaataaaga atctttaaca ggtataaaac
     1621 ggacaatcca atccaaagaa tatgagaact gctatgtttt tagcgatacc atcgacatta
     1681 aagaattgat ttatgtaaaa gactatggcg ttgttaaact ggaactatta aatggatact
     1741 ctattgaatt aataagaaaa ataaaatgaa acatataata tcactattgc ttgttttggc
     1801 aaccttacaa ctggttgcac aaaccacctt gaactttcgg acaacgatgt tcaacaacct
     1861 caccacatcg aagctttcga ctaatgtgct ggccaacaag gccggaatgc ccgattccat
     1921 ttttgggttc aatggcagtt ctacctgcaa tgcgtcaact ttcgcaagct ggcaatatgc
     1981 ttatcaatcg ctctatgtgg ggtttgtggg tacgccgtct tatccaacac cagaaaccat
     2041 actggcacaa gtacccgata gccccacttc cgaaattaag ataaacggcc tctattacaa
     2101 atacaaccac ttggccgaca atgctgtttc gagtggattg atccacctca ccgacacgct
     2161 gttttacgat ggccccaaca gcggcaaccc ttacttagaa aaaacatgcg tagcggttag
     2221 cccgatgtat cctgccgaag tccccttcaa tgtaatattc cgtggtggtg ccatttacgg
     2281 caaccttacg gccaatgttg catcggccag tatcgacttt ggcaatggca gtggatatgt
     2341 atcatttgta gccaacaatc tatacgacat cagctattcg agcgaag
//
LOCUS       DAOPUW010000285         2385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_47042_length_2385_cov_2.798712, whole genome shotgun sequence.
ACCESSION   DAOPUW010000285 DAOPUW010000000
VERSION     DAOPUW010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47042_length_2385_cov_2.798712"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..1191)
                     /locus_tag="PKJ43_05665"
     CDS             complement(154..1191)
                     /locus_tag="PKJ43_05665"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNZ72089.1"
                     /translation="MKRNKKILLAIAIVVPALVVLYSYLQQLVDYLIYGLIGLNPESH
                     LGASVNFFFYDTIKILILLFLISSLMGVVNAYFPIDRLRNYLNTKKMYGLQYLLASLF
                     GAITPFCSCSSIPLFLGFVKGGIPLGVTFSFLITSPLVNEVAVAMFLGLFGVKATLVY
                     AISGILLGTIGGFILGQFKLERYLSNWVKEIQIQSDQETEAWEAEHTPFIDRLPGIVR
                     EGWDIVRKVLVYVLIGIAIGAAMHGYVPEDFFTTYLSADRWYAVPVAVILAVPMYANA
                     AGIVPVIQVFVAKGVPLGTAIAFMMAVVGLSLPEATLLKKVMTWKLIGIFFGTITIFI
                     ILLGYLFNWIL"
     gene            complement(1242..1943)
                     /locus_tag="PKJ43_05670"
     CDS             complement(1242..1943)
                     /locus_tag="PKJ43_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HNZ72090.1"
                     /translation="MEVLQNFLENSQFPLLSAFILGLMTAISPCPLATNITAIGFISK
                     DIESRRKVFYNGLIYTLGRAITYTTIGLIFFFSAGEIRMSGFLQQWGEKLLGPLMIII
                     GLFMLGAFNFIKIPGLGSLTDKMENRANKGFWGVLLLGIVFALAFCPYSGVLYFGMLI
                     PMTISSASGLYLPVVFAIATGIPVILFAWLIAFSVGSIGGVYNKLKIFEIWFRRVVAV
                     LFMGVGIYYVVRLYF"
     gene            complement(1953..2333)
                     /locus_tag="PKJ43_05675"
     CDS             complement(1953..2333)
                     /locus_tag="PKJ43_05675"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HNZ72091.1"
                     /translation="MKKLIALFSVIALFATTSVYAQKAEKEAIEAYYFHYTSRCVTCQ
                     SVEKVSSDALKELSGGKIVLKSINLDDKSNKELAKKLKIDGQTLLVVKGVKKVDITND
                     GFLYATTSPEKLKAKLKTAVESLK"
BASE COUNT          759 a          530 c          466 g          630 t
ORIGIN      
        1 gggttcgaat cgtaaaccac cacgctttgc agttcaattt cggcgttggt aaacgctttg
       61 aggaaaattt tggtgatgcg gccaccaccg ataaaaccaa gggcatttgt tttcatatta
      121 aattgatttt aaggttattc aaaagacatt aagttacaat atccaattaa acagatatcc
      181 caacaatatg atgaaaatgg taatggttcc gaagaaaata ccgatgagtt tccaggtcat
      241 cacttttttg agcagggtag cttcgggcaa cgaaagtcca accacggcca tcataaaggc
      301 aatagctgtc cccagcggaa cacctttggc tacaaacacc tgaataacgg gaacaattcc
      361 ggcggcattg gcatacatag gtacggccaa gattactgct acaggaacgg cgtaccatct
      421 atcggccgaa aggtatgtgg taaagaaatc ttcgggaacg tatccgtgca tggcggcacc
      481 tatggcgatt ccaatcagca cataaacaag tactttccgc acaatatccc agccttcgcg
      541 cacaattccg ggcagccggt caatgaatgg cgtatgttcg gcttcccagg cctcggtctc
      601 ctggtcgctt tgaatttgaa tttccttcac ccagttgctc aggtatctct caagtttgaa
      661 ttgcccaagg atgaaaccac ctattgttcc caacaaaatt ccactaatgg cataaaccaa
      721 tgtggctttc acaccaaaca acccaagaaa catggctacg gccacttcgt tcaccaaggg
      781 cgaggtgatc aaaaacgaga acgtaactcc aagcgggatg cctcctttta caaaccccag
      841 aaacagcgga atcgacgaac aggaacaaaa cggggtgatt gcaccaaaca acgaagccag
      901 aaggtattgc aatccataca ttttttttgt gttcaggtag ttacgcaacc tgtcgatagg
      961 aaagtatgca ttcacaaccc ccattaacga actgatcaga aatagcagaa tcaggatttt
     1021 aatagtgtcg tagaaaaaaa agttgaccga agcgcccaaa tgtgactcgg gattcaatcc
     1081 tataaggcca taaatcaggt aatcaacaag ctgttgaaga taactataca gcactaccag
     1141 tgcaggaacg acaatggcta tggccaatag tattttttta tttcgtttca tgttattcta
     1201 ttcgttgttc gcaaaaacac gaacaattat ttgaaatttt tttaaaaata taaccttaca
     1261 acatagtaaa tacccacacc cataaaaagc acagcaacca cacgacggaa ccatatctcg
     1321 aagattttca gcttattgta gaccccgcca attgaaccca cagagaaagc aatcaaccat
     1381 gcaaatagga ttacaggtat tccggtagct atcgcaaaaa ctacaggcag gtataaaccc
     1441 gaagcactac tgattgtcat gggaataagc atcccgaagt aaagcacacc gctgtaaggg
     1501 cagaaggcca atgcgaaaac aattcccaat aagagaacgc cccagaaacc tttgttggcg
     1561 cggttttcca ttttgtcggt taaagaacca agtccgggaa ttttgatgaa gttaaaagca
     1621 ccaagcatga acaatccaat gatgatcata agaggcccca gaagcttttc tccccattgc
     1681 tgtagaaaac ctgacatgcg gatttcacca gcgctaaaaa agaagattag cccaattgta
     1741 gtgtaggtaa tggcccgccc aagtgtatag atcaaaccgt tgtaaaatac ctttctacgg
     1801 ctttcgatgt ctttgctgat aaaaccaata gccgtaatgt tggtggctag cgggcaggga
     1861 ctgatggcgg tcattagtcc caatataaat gctgacaaca acggaaactg cgaattttct
     1921 aggaaatttt gtagtacttc cattgcttta aactatttca acgactcaac agccgttttc
     1981 aactttgctt tcagtttttc tgggctggta gtggcataca aaaagccatc gttggttata
     2041 tcaacctttt taacgccttt gacaacaagt agtgtttgac catcaatttt aagttttttg
     2101 gccaattcct tattgctttt atcatcgaga ttgattgatt ttaacacaat tttacctcct
     2161 gaaagctcct tcaaagcatc ggaagaaacc ttttcaacag attgacaagt tacacaacgg
     2221 ctggtataat gaaaatagta agcttcaata gcctcttttt ctgctttttg tgcataaacc
     2281 gatgtggttg caaataaggc tattacacta aataatgcga tcaatttctt catagtctat
     2341 aattattttg tgattaaact tttaatttca tccaggctcg gaact
//
LOCUS       DAOPUW010000286         2383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_47084_length_2383_cov_3.523196, whole genome shotgun sequence.
ACCESSION   DAOPUW010000286 DAOPUW010000000
VERSION     DAOPUW010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47084_length_2383_cov_3.523196"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1896)
                     /locus_tag="PKJ43_05680"
     CDS             complement(<1..1896)
                     /locus_tag="PKJ43_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72092.1"
                     /translation="METKKYIETRKQPMRLAFIPFLVLVSLFLLKTIPGKAVVKEIGV
                     PAIQNYPRSQYNAGTQNWCTEQGSNGIMYFGNNNGLLRYDGSFWELHAVPNGSNVRSL
                     HFSDTQQLLFAGAFNEFGYFSNDSTGQLTFKSLVSLVPEEYRNFGDIWKIYEGPWGIM
                     FQAFEAMFFYQNNSIEVVLPRSIFHFSYFVNGNLYVFDRNNGLMEYRNGYLKKIPSGD
                     FFIQNEVWSVLPLNNDELLIGTASDGLFVYDGVTLKAWNTPVNSLLKQHQIYSSQVID
                     NNHFAFGTIQNGLIICDKKGEIVQSINREKGLQNNTILSMNIDLDGNLWLGLDNGIDY
                     VEINSPITLLQDYYEFGTGYVSILHNGLLYIGTNQGLFYCNPARFSSATLNASDFNMV
                     ENTSGQVWSLQQIEGKLYCGHNNGCFLIEGNRGKQINEVPGCWALNELPGYPNVYIEG
                     TYNGAELFRYNNGQMEHITNIKGVNYSCQEMVCLKDRYVWISHSFKGASLFRLNQNLD
                     SLELIRKFGAADGLPSDYLNKIKRMKNGDILLITPKGIYQFDFNTNTFSRSTKYNSLF
                     DNQPVVYLQEDEKNNIWYINTNNEGGVLRLQEDGTFSNVSYPFYKLKGKFIGSFFHFN
                     VIDEENVL"
     gene            complement(2035..>2383)
                     /locus_tag="PKJ43_05685"
     CDS             complement(2035..>2383)
                     /locus_tag="PKJ43_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNZ72093.1"
                     /translation="YTSPEISSVGLTESKAIAAGYEINVGKFPFTASGKASAAGMKDG
                     FVKLVFDAKYGELLGAHMIGGNVTEMISELVVARKLEITGHELLKTIHPHPTMSEAIM
                     EAAAAAYGEVIHL"
BASE COUNT          676 a          490 c          472 g          745 t
ORIGIN      
        1 gagcacattt tcttcatcga tgacattaaa atggaaaaaa ctaccgataa acttcccttt
       61 caatttgtaa aatgggtacg aaacgtttga aaatgtacca tcttcctgta gcctgagtac
      121 gccaccttcg ttgttggtat tgatgtacca aatattgttt ttttcatctt cctgaaggta
      181 aactacgggt tgattatcaa acaacgagtt atactttgtc gatcgtgaaa atgtgtttgt
      241 attgaagtcg aactgatata ttccttttgg ggtaatgagg aggatatctc cgtttttcat
      301 ccgtttaatt ttgttgagat aatcggaggg aagaccgtcg gcagcaccaa attttctgat
      361 tagttccaaa ctatcgagat tttgatttag cctgaaaagg cttgcgcctt taaatgagtg
      421 gctaatccac acataacgat cttttaaaca aaccatttcc tggcaagagt aattcacccc
      481 tttgatgttt gttatgtgtt ccatctggcc attattatac cgaaagagtt cagccccgtt
      541 gtaggttccc tcgatatata cgtttgggta gcctggtaat tcgtttagtg cccagcatcc
      601 aggcacttcg tttatttgtt ttcccctgtt tccttcaata agaaaacacc cgttattgtg
      661 tccgcaatat agctttcctt caatttgttg caggctccaa acttgtcccg aagtgttctc
      721 aaccatgttg aagtcgctgg catttaaggt agcgctcgag aaacgtgcgg gattacaata
      781 aaaaaggccc tgattggtgc caatatagag aaggccgtta tgaagtattg aaacatagcc
      841 ggttccaaat tcgtagtaat cctgaagcaa agtaataggt gagttaatct caacataatc
      901 aataccattg tcgaggccaa gccaaaggtt tccatcaagg tcgatattca tactgaggat
      961 tgtgttgttt tgcaagcctt tttcgcggtt tatgctttgt acaatttctc cttttttgtc
     1021 acaaattatc agtccgttct gaatagttcc gaaggcgaaa tggttattgt cgataacctg
     1081 actggaatat atctggtgtt gttttagcaa tgaattcaca ggggtgttcc aggctttcag
     1141 tgttaccccg tcgtaaacaa aaaggccatc gctggcagtt ccaataagca gttcatcatt
     1201 gttaagcggc agcaccgacc aaacttcatt ctggataaaa aagtcgcccg aagggatttt
     1261 cttcaaatat ccgtttcgat actccattaa gccattgttg cggtcgaaaa catatagatt
     1321 tccatttaca aaataggaaa aatggaaaat acttcgtggc aaaacaactt cgatactatt
     1381 gttctggtag aaaaacatgg cctcgaatgc ctgaaacata ataccccacg ggccttcata
     1441 tattttccat atatctccaa aattccgata ttcttctggg acaagtgaaa ctagggattt
     1501 gaaggtcagc tgcccggtag agtcgttgct gaaataccca aattcgttaa atgccccggc
     1561 aaacaggagt tgctgagtat cggaaaaatg taatgaacgt acatttgacc catttggaac
     1621 ggcatgcaat tcccaaaaac ttccatcgta gcgaagcaag ccattattat ttccaaaata
     1681 cattatcccg ttgctgcctt gttcggtaca ccagttttga gtgcctgcat tatattgaga
     1741 gcgaggataa ttctgaattg ccggtacgcc aatctctttc acaaccgctt tccccggaat
     1801 tgttttcaat aaaaacagcg atacaagaac aagaaatggg ataaaagcca gccgcatggg
     1861 ttgctttcgt gtttcgatgt atttttttgt ttccataatc attgggctaa aaagtatctc
     1921 aatataatca aatttgaatt aattataatc aagaattttg tatggtttcc ggaaacaaaa
     1981 tagggttgaa agaataaacc tttcaacccg ttttttatat agacaaagta ttaattacaa
     2041 gtgaattact tctccatacg ctgcggcggc agcttccata attgcttcgc tcattgtggg
     2101 gtgcggatgt atcgttttga gcagttcatg accggtaatt tcgagttttc gggctactac
     2161 aagttctgaa atcatttcag ttacgttccc tccaatcatg tgagccccca aaagttcacc
     2221 atattttgcg tcgaaaacga gtttcacgaa tccatctttc attccggcag cacttgcttt
     2281 tcccgatgct gtgaatggga attttccaac gttaatttca tatccggcag caatggcttt
     2341 cgactcggta agcccaaccg acgatatttc aggcgatgta tag
//
LOCUS       DAOPUW010000287         2381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_47153_length_2381_cov_2.931212, whole genome shotgun sequence.
ACCESSION   DAOPUW010000287 DAOPUW010000000
VERSION     DAOPUW010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47153_length_2381_cov_2.931212"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..1201
                     /locus_tag="PKJ43_05690"
     CDS             59..1201
                     /locus_tag="PKJ43_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNZ72094.1"
                     /translation="MKNLFVILALLAQTSMVSLGQVVENSIPAPTNINENQCPCIMPD
                     NSVVFQVKAPNAQKIQIDLGKKYDMVKGADGVWTVRTEPVEPGFHYYFLVIDDVPVAD
                     PASQSFFGTGKMSSGIDIPGKGADFYAIKDVPHGAISSKYYYSNVSESWRRLYVYTPS
                     GYDQDKNAKYPVVYIQHGGGEDETGWVIQGKTDLILDNLIAEGKAKPMIVVIANGNVR
                     AGGGGYSSQGMAAFKKEMIQNIVPFIDKNYQTLTDKKNRAICGLSMGGGQSFYVGLES
                     LDVFGSVGIFSSGIFGGIRNPSGSTFDAEKEIPGLLSKSADFNKKLDLFYISCGEGDM
                     RIEHTKKAVATMKEKGLNVAFNSFSFIVATAFLVCSMRISKKPWLL"
     gene            1373..>2381
                     /locus_tag="PKJ43_05695"
     CDS             1373..>2381
                     /locus_tag="PKJ43_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNZ72095.1"
                     /translation="MNKLGTYVVGLLLVAPFCLRAQQANVNLDYNPQKDTEGLIPFSA
                     PLNSPEVHDDNTVTFRLKAPSAQKVVLPTGAIYTANNLGNTPLEFTKGVDGIWTLTIG
                     PLKPDMYAYHFNIDGVQVPDPSNTYAAFTAMPPYSELVIHGSEPAYYDAKNVPHGNVT
                     RHVYHSEVTKGEREMYVYTPPGYDAKKKYPVLYLVGGSGELPSNWVFDGRVNFIMDNL
                     LAEGKVVPMIIAIPNNQVVHRNHPKHAELTFDIFEKELREHVIPLVDKSYSTIASPKG
                     RAISGLSMGGRHSMFIGFRSLDVFANFGILSAGDDKPETTLANFLTDPKVNDKVDYLF
                     IG"
BASE COUNT          664 a          503 c          552 g          662 t
ORIGIN      
        1 aactgatagt caatatatta attcattatt ttcataaatt taaacagttt ctaaaaatat
       61 gaagaattta tttgtgattt tggcgctttt ggctcaaaca agcatggtta gtttgggaca
      121 ggtggttgag aactcgattc ctgcgcctac gaacattaat gaaaatcagt gtccgtgcat
      181 aatgcccgac aacagtgtgg tttttcaggt gaaggcgcct aatgcacaaa aaattcaaat
      241 tgatttagga aaaaaatacg acatggtaaa aggtgcggac ggcgtttgga ctgttcgcac
      301 cgaacctgtc gaaccgggtt ttcactatta ctttttggtg atcgacgatg tacctgttgc
      361 cgatccggcc agtcaatcgt tctttggtac aggtaaaatg tccagcggta tcgatattcc
      421 gggaaaaggt gccgattttt atgccatcaa agatgtcccc catggcgcca tcagttcaaa
      481 atattactat tcaaatgtaa gcgaaagctg gcgacggctg tacgtttata cgccttctgg
      541 ttacgatcag gacaagaacg caaaatatcc ggtggtgtac attcagcacg gtggtggtga
      601 ggacgaaacc ggctgggtaa tccagggcaa aaccgacctt attctcgaca atttgattgc
      661 cgaagggaaa gccaaaccca tgattgtggt gattgccaac ggaaatgttc gcgcaggcgg
      721 aggtggatac agcagccagg gtatggccgc attcaaaaag gaaatgatcc aaaacattgt
      781 gccatttatc gacaaaaatt accagaccct taccgacaaa aagaatcgtg ccatttgcgg
      841 cttgtccatg gggggcggtc agtcattcta tgtagggctc gaaagccttg acgtttttgg
      901 ttccgttgga attttcagct caggcatctt tggaggcatc cggaatccat cgggaagcac
      961 tttcgatgct gaaaaagaaa ttcccggatt actcagcaaa tcggcggact tcaataaaaa
     1021 gctcgatttg ttctatatct catgtggcga gggcgatatg cgcattgaac ataccaaaaa
     1081 agccgtggct actatgaaag aaaaagggtt gaacgttgcc ttcaattctt tttctttcat
     1141 agtagccacg gcttttttgg tatgttcaat gcgcatatca aaaaagccgt ggctactatg
     1201 aaagaaaaag ggttgaacgt tgccttcaat tctttccccg gcgaccacga gtggcaggtt
     1261 tggcggaaat cgctgcatga ctttgcttcc cgggttttta agtaaattgt tgcacctgag
     1321 ttaaatcaat gcaataatgt tgtgatacaa aaaacagaca aaatactata ctatgaataa
     1381 attaggaacc tatgttgttg gccttctttt agttgctcct ttttgtttaa gggcacagca
     1441 agccaacgta aaccttgatt acaatcctca aaaggatact gaaggattga tcccgtttag
     1501 tgctccgtta aattcgcctg aagtgcatga cgataacacg gttacttttc gattgaaagc
     1561 acccagtgcg caaaaggtgg tcttgccaac cggagccatt tatacggcca acaatttggg
     1621 caatacaccc cttgagttta ccaaaggtgt cgatggtatt tggacactca ccattggccc
     1681 gttgaaaccc gatatgtatg cctatcattt taatattgac ggcgttcagg ttcctgatcc
     1741 cagcaatacc tacgctgctt ttacggccat gccgccttac agtgagctgg ttatccatgg
     1801 tagtgaacct gcctattacg atgctaaaaa tgttcctcat ggcaatgtaa cccgccacgt
     1861 ataccactcg gaagttacca agggcgaacg tgaaatgtat gtgtatacgc caccgggcta
     1921 cgatgctaaa aagaaatatc cggtgttgta ccttgtgggt ggtagtggcg agctgccttc
     1981 caattgggta ttcgacggaa gggtcaactt cattatggac aacctgttgg ctgaaggaaa
     2041 agttgttcca atgatcatcg ccattcccaa taatcaagtg gttcaccgca accatccaaa
     2101 acatgccgag ttgactttcg atattttcga aaaggaactt cgtgagcatg taatcccgtt
     2161 ggttgataaa agttacagca ctattgcatc gcctaaaggg cgtgccattt cgggtttgtc
     2221 gatgggtgga cgtcacagta tgttcatagg tttccgctcg ctcgatgttt ttgccaactt
     2281 tggtatactt agcgctggcg acgataaacc agaaaccact ctggctaact ttttgactga
     2341 tcccaaagtg aatgataaag tcgattattt attcattgga c
//
LOCUS       DAOPUW010000288         2378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_47255_length_2378_cov_2.871718, whole genome shotgun sequence.
ACCESSION   DAOPUW010000288 DAOPUW010000000
VERSION     DAOPUW010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47255_length_2378_cov_2.871718"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="PKJ43_05700"
     CDS             complement(<1..1232)
                     /locus_tag="PKJ43_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNZ72096.1"
                     /translation="MILNYIWICFFLIAFVVALAKLIFFQDVTVFSALVTSTFDAAKT
                     GFEVSLGMTGVLTFWMGIMKVGEKGGVVQALSRAIGPFFQRLFPELGRNHPAYGAITM
                     NIAANMLNLDNAATPMGLQAMKEMQASNPSKDTASNAQIMFMVLNASGLTIIPVTIMV
                     YRAQLGAANPTDVFIPILIATFCSTLAGLIAVSVYQRINLLDKVILSWLGGMLAVMIG
                     IIWYFASLEGDQVQVVSNLLSGIILVLVIIGFILMAMLRKVNVYESFIEGAQEGFSTA
                     VKIIPYLIAILVAIAMFRASGAMDYFIAGVKWCFDVLHINTDFVDALPTALMKPLSGS
                     GARGMMIDAMKTHGADSFVGRLASTFQGSADTTFYILAVYFGSVGIKKTRHALACSLI
                     ADLVGFIAAIFVAYFFFH"
     gene            1557..2306
                     /locus_tag="PKJ43_05705"
     CDS             1557..2306
                     /locus_tag="PKJ43_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNZ72097.1"
                     /translation="MKYTKKISFFILVLVLFSTTAFSQDKFFASVSMGKVFPLGQFAS
                     NNLESKNSGFSKDGFTLSVDGDYQLHRRWAASLRLNLMNTTFNEESVIQLLKNEMSNF
                     TKATDSVQFDISYWLWISPMVGVKYNYPIIINKLYVEAGIFTGVNFVQIPDQNVYFND
                     KEKERTVFSKNMGETDVTFPITANIAFRYRLNKRMQLKINSEYFSTRTAFDHKTFYRM
                     NKGTEAVELNGYHVNVPIKTMNITLGLVYNL"
BASE COUNT          846 a          495 c          419 g          618 t
ORIGIN      
        1 caatgaaaga agaaataggc aacaaaaatt gcagcaataa aaccaactaa atcggcaata
       61 aggctgcaag ccaatgcatg acgggttttc ttaattccaa cagaaccaaa atacacagcc
      121 aagatataaa aagttgtatc agctgaacct tgaaatgtag aagcaagacg acctacaaag
      181 gaatcggcac catgagtttt catggcatcg atcatcattc cccgagctcc gctaccgctc
      241 aatggcttca ttaaggcagt tggaagtgca tcaacaaaat cggtgttaat atggagaaca
      301 tcgaaacacc acttcacccc ggcaataaaa taatccattg cccccgaagc ccgaaacatg
      361 gcaatggcta ccagaatagc aatcagatat ggaattattt tcaccgcggt gctgaaacct
      421 tcttgtgctc cttcgataaa agattcataa acattgactt ttcggagcat tgccatcaga
      481 ataaaaccaa ttattactaa aaccaggatt ataccactga gaagatttga aacaacctga
      541 acctgatcgc cctcaagcga cgcgaaatac caaataatac ctatcatcac agccagcatt
      601 ccgcctaacc aacttaaaat tactttgtcg agcaaattaa ttcgctgata aacagaaaca
      661 gctataaggc cagccaaggt agaacaaaaa gttgcaatta agataggaat aaatacatca
      721 gttggatttg cagcacccaa ttgtgccctg taaaccataa tagttactgg aataattgta
      781 agccctgaag cattgagcac catgaacatg atttgcgcat tacttgcagt atctttcgag
      841 ggattagagg cttgcatttc tttcatcgcc tgcagtccca ttggcgttgc agcattgtcg
      901 agattgagca tgttggccgc aatgttcatg gtaatagccc cataagccgg atgatttctc
      961 ccaagttcgg ggaacaggcg ctgaaagaat ggcccgattg cccgtgaaag tgcctggact
     1021 accccaccct tttctccaac tttcattatc cccatccaaa aagtgagcac tcctgtcatg
     1081 cccaacgaca cctcaaaacc tgtctttgcc gcatcgaaag ttgaagttac taacgcagaa
     1141 aaaaccgtaa catcttgaaa gaatatcagt ttcgcgagag caactacaaa tgcaatcaga
     1201 aaaaaacaaa tccaaatata attcagtatc atagcgtagg ataaagaggt ttaaacaatt
     1261 accccaacgg gtatcagaac tcaaaactac aaaaattaat attcagccat acattgtggc
     1321 tttttgtttt atcaacaatc gatttttgat aaaaaaggac aaaaaatcca tcgccaccaa
     1381 tattaaacaa cagtctggaa acgttcttcc aaagacgggt aagcataaat cataaaaaca
     1441 tcaattaact aaacaacaat attttaaaaa gaatgaatat ccctgttaat aaatactaaa
     1501 gtagaataag tatgaaaaaa attactttta tttgaacttg aattatcttt atcgtcatga
     1561 aatacacaaa gaaaataagc tttttcatct tagtcttagt actgttttca acaacagcct
     1621 tttcgcaaga taagtttttt gcatcggttt cgatgggaaa agtatttcct ctaggccaat
     1681 ttgcatccaa caatcttgaa tctaaaaaca gtggtttttc aaaagatggc tttacccttt
     1741 ctgttgacgg cgattaccag ttacatcgac ggtgggcagc ttcgctaagg ctgaatctga
     1801 tgaatacaac atttaatgag gaaagtgtga tccagttgct gaaaaatgag atgagcaatt
     1861 tcacgaaagc aacagacagt gtacagttcg acattagtta ctggctgtgg atttctccaa
     1921 tggtaggtgt aaaatacaac tacccaatca ttatcaacaa attatatgta gaagcaggaa
     1981 ttttcaccgg agtcaatttc gtgcagatac ctgaccaaaa tgtatacttc aacgacaaag
     2041 aaaaagagcg gactgttttc tctaaaaata tgggagaaac tgatgttacg tttcctatta
     2101 ctgcaaatat tgcgtttcgt taccgcctga acaaacgtat gcaattaaaa atcaacagtg
     2161 aatattttag tacacggact gcattcgacc ataaaacttt ttatcgaatg aacaaaggaa
     2221 ccgaagctgt tgagttaaat gggtaccatg tcaatgttcc cataaaaacg atgaatatta
     2281 ccctggggtt agtttataat ttataattaa ggcaaggctc cacaacatcg cacaaaaaag
     2341 ctatttttgg ccgctgaaaa tgaaaaccga tcatcaaa
//
LOCUS       DAOPUW010000289         2366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_47620_length_2366_cov_3.635656, whole genome shotgun sequence.
ACCESSION   DAOPUW010000289 DAOPUW010000000
VERSION     DAOPUW010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47620_length_2366_cov_3.635656"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PKJ43_05710"
     CDS             complement(<1..592)
                     /locus_tag="PKJ43_05710"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HNZ72098.1"
                     /translation="MKRSIIEFIDELNVELDNILNFWSKQALDTQRGGFYGKILSSGV
                     RVKDANKGAILNARILWSFSAAYRYTKKQQHLEMAERAFNYLVDYFIDKKNGGVFWEL
                     DSKGNPVNTRKQAYAQGFALYGLSEYYMASGNERSLQLAQELFWTIEKHFLEKQFGGY
                     IEALTEDWTPLDDVRLSTKDANEPKSMNTHLHIIEPY"
     gene            complement(594..1769)
                     /locus_tag="PKJ43_05715"
     CDS             complement(594..1769)
                     /locus_tag="PKJ43_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HNZ72099.1"
                     /translation="MSTIFETRKEAIEKLHSELLVRKNEELFSVNGIYCRFKYPVLTR
                     DHAPLHWRFDFNPKTNPFFMERIGINATFNSGAIKWNGKYLLVVRVEGNDRKSFFAIA
                     ESPNGIDNFKFWDRPITIPQTDNWETNVYDMRLTAHEDGWVYGVFCAERKDPDAPDGD
                     TSSATAAAGIVRTKDLISWERLPDLISHGGQQRNVVLFEKFVDGKYAFFTRPQDGFID
                     TGKGGGIGWGLSDTIENAEIPNEEIIDPKTYHTVYEVKNGLGPHPIKTAKGWLHLCHG
                     VRNTAAGLRYVLYVCMADLNDVSKITHKPHGHFIAPLGGERLGDVSNVVFSNGWIADD
                     DGTVLIYYASSDTRLHVAKTSIDQLVDYCLNTPSDELYSHLSVQTINKLIDNNQKFL"
     gene            complement(1811..>2366)
                     /locus_tag="PKJ43_05720"
     CDS             complement(1811..>2366)
                     /locus_tag="PKJ43_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNZ72100.1"
                     /translation="GGWFWWGNTSCSEAEYTALFRFTVDYLQKTKKINNLLIVFSPDH
                     NFESAEQYLARYPGDDYVDILGLDNYSDFKEQRLDRVVVKLGIVSDIAAQKGKIAAFT
                     ETGNDRLTIPDWYTSNLLQVLNASEKTRRMAYVMVWRNHDTTHFYVPYPGHREEEDFR
                     NFVNNKMIFLIDDLTKQYEYQFSK"
BASE COUNT          670 a          545 c          440 g          711 t
ORIGIN      
        1 tgtaaggctc aataatatgg agatgggtat tcattgactt tggttcgttt gcatctttgg
       61 tactaagccg cacgtcatcg agcggagtcc agtcttcggt cagcgcttcg atatatccgc
      121 caaactgctt ttcgagaaaa tgcttttcaa tcgtccagaa aagttcttga gccaactgca
      181 aacttctttc atttcccgaa gccatataat attctgaaag tccataaagt gcgaagccct
      241 gggcatacgc ttgctttcgg gtattaaccg gattaccttt cgaatcgagt tcccagaaaa
      301 cacccccatt ttttttgtcg atgaaataat cgaccagata attaaatgcc cgctcggcca
      361 tttcgagatg ctgctgtttt tttgtatagc gatacgctgc agaaaatgac cacaaaatcc
      421 tggcattcag tatagcaccc ttatttgcat cttttaccct aacccccgac gaaaggattt
      481 taccataaaa ccccccacgc tgtgtatcca aagcctgttt gctccagaaa tttaatatat
      541 tatcgagttc aacatttagc tcgtctataa attcaataat tgatcttttc attctataga
      601 aacttttggt tgttatcaat cagtttgtta atagtttgaa cagaaaggtg cgaatacaat
      661 tcatccgaag gggtattcaa acaataatca accaactgat cgatagaagt tttagctaca
      721 tgcagacggg tatccgatga agcataataa atcaatacgg ttccatcgtc gtcggcaatc
      781 cacccgttcg agaaaacaac attcgataca tcgccgagac gttcaccccc aagtggcgca
      841 ataaaatggc catgaggttt atgcgttatt ttcgaaacat cgttcaggtc ggccatgcat
      901 acatataaca cgtaacgcaa gccggcagca gtgttgcgca ctccatgaca caaatggagc
      961 cagcctttag cggttttaat agggtgaggg ccaagaccgt ttttcacctc gtaaaccgtg
     1021 tggtatgttt tgggatcaat tatctcttcg ttcgggattt cggcattttc aattgtgtca
     1081 gataaacccc agccaattcc tccccctttt ccggtgtcga taaaaccatc ctgaggccgg
     1141 gtaaagaaag catatttacc atcgacaaac ttctcgaaaa gcacaacatt tcgttgctgt
     1201 cctccatgag aaatcaaatc gggaagacgt tcccagctga tcaaatcttt agtacgtaca
     1261 ataccagctg cagcagtggc gcttgaagta tcaccgtcgg gagcatctgg atctttgcgt
     1321 tcggcacaga aaaccccgta aacccatcca tcttcatgag cagtcaggcg catgtcgtat
     1381 acgttggtct cccaattatc ggtctgagga atagttattg gtctgtccca gaacttgaag
     1441 ttatcgattc cgttggggct ttctgcaatg gcaaaaaaag atttccgatc gttgccttca
     1501 accctcacta ccaacaaata cttgccattc cactttattg ccccggaatt aaacgtagcg
     1561 tttatcccaa tgcgttccat aaaaaacgga ttggtttttg ggttaaaatc gaaccgccaa
     1621 tgaagtggag catgatcgcg cgttaatacg ggatatttaa atcgacaata tattccgttt
     1681 acgctgaata gctcttcgtt tttacgaacc aataactcgc tgtgtaactt ctcaatagct
     1741 tcttttcttg tttcaaatat ggtactcata taataattta catctaatta tttcttgtga
     1801 cttatttttt tcatttagaa aattggtatt catattgttt tgtaagatcg tcaatcaaaa
     1861 atatcatttt attgttaaca aaattcctga aatcttcttc ttccctatgt cctggatatg
     1921 gcacataaaa atgggttgta tcgtggttac gccaaaccat aacatatgcc atccgtcgtg
     1981 ttttttcact ggcattcaac acctgcaata aattagaggt ataccagtcg ggaatggtca
     2041 acctatcatt tcctgtttcg gtaaaagcgg caattttccc tttttgagct gcaatatccg
     2101 acacaattcc caattttaca accaccctgt cgagccgctg ctctttaaag tcggaataat
     2161 tgtcaagccc cagaatatcg acataatcgt cgccgggata acgggcaaga tactgttctg
     2221 ccgattcgaa attatgatcg ggtgaaaaaa caatcagtaa gttatttatc ttctttgtct
     2281 tctgtaaata atcaacagta aaacggaaaa gcgctgtata ttctgcctct gaacaacttg
     2341 tgtttcccca ccagaaccat ccccca
//
LOCUS       DAOPUW010000290         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_47896_length_2356_cov_3.813125, whole genome shotgun sequence.
ACCESSION   DAOPUW010000290 DAOPUW010000000
VERSION     DAOPUW010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47896_length_2356_cov_3.813125"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..808
                     /locus_tag="PKJ43_05725"
     CDS             29..808
                     /locus_tag="PKJ43_05725"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HNZ72101.1"
                     /translation="MKSKEEIVNNWLPRYTGVELENFGRYVLLTNFQHYVDLFARYFN
                     VPVVGEDLNMPSATANGITILNFGMGSPNAATAMDLLSAISPRAVLFLGKCGGLKVKN
                     KVGDLILPIAAIRHDGTSDDYLPPEVPALPAFNLQRAVSSSIRDRGRDYYTGVVFTTN
                     RRVWEYDDRFKKYLRKTRAMAIDMETATIFITGFANQIPTGALLLVSDQPMISTGVKT
                     TESDKSVTAKYVDEHVEIGIRAMEMLRDNGSSVKHLKFFDE"
     gene            805..1803
                     /gene="holA"
                     /locus_tag="PKJ43_05730"
     CDS             805..1803
                     /gene="holA"
                     /locus_tag="PKJ43_05730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNZ72102.1"
                     /translation="MTHTEIIENLKKKIYRPVYVLMGEEPYFIDLISDYIADNVLPES
                     EKSFNQTILYGKDTTSDMLITIARRFPMMSAYQVVILKEAQLFDKLEDELLSYIENPL
                     NSTILVICYKYKTLDKRKKFLKVASEKGIVFESPKIYENQLGAWISKYIGDNGFKIAP
                     QANAMLAEYLGTQISKVANELDKLMILLPKGATITPADVEKNIGISKEYNIFELQNAL
                     GERDIIKSNRIVNYFASNKQTPFPLVIGGLGQYFAKLLKYHFLSDKSKNNAAKALGVS
                     PYFVDTYIKAAKYYSPKKLVEIISMLREFDMKSKGIGNVSSTEGDLLRELTYKILH"
     gene            1805..>2356
                     /locus_tag="PKJ43_05735"
     CDS             1805..>2356
                     /locus_tag="PKJ43_05735"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNZ72103.1"
                     /translation="MLLLIILLTSVISVMAFSNAKISETLSLKPVLVYHNKQVYRAIT
                     HSLIHADWAHLLVNMFVLFSFGQACLAYFNQFFGTASNTYFIILYIGSLIFSSLFSIY
                     KHRNNPYYTAVGASGAVMAVVFTSIFFDPWNMLYFFGIIPIPGIVFGFLYMGYSIYMG
                     RKGNDNIGHDAHFFGALFGFLYPL"
BASE COUNT          721 a          509 c          454 g          672 t
ORIGIN      
        1 tatttttaca aaaaacaaaa gaccgtgtat gaaatcgaaa gaagaaatag taaacaactg
       61 gctaccccga tacaccggtg ttgagcttga aaattttggg agatatgtat tactcaccaa
      121 ctttcaacat tatgtcgatt tgtttgcccg atatttcaac gtgcctgttg ttggcgaaga
      181 tctcaacatg cccagtgcca ccgctaacgg gatcactatt ttgaactttg gcatgggaag
      241 ccccaacgcg gccaccgcca tggaccttct tagtgcgatt tcgcctcgtg cagtcttgtt
      301 cctaggcaaa tgcggtgggt tgaaggttaa aaacaaagta ggcgacctta ttctgcccat
      361 tgctgccatt aggcacgacg gaacatcgga tgattatctt ccacccgaag ttcccgctct
      421 gccagccttt aacttacaac gagctgtctc atcatcaatc cgtgatcgcg gacgcgacta
      481 ttataccgga gtggttttca ccacaaaccg cagggtttgg gaatacgacg accgttttaa
      541 aaaatacctt agaaaaaccc gcgctatggc cattgacatg gaaacagcaa ccattttcat
      601 tacgggtttt gccaaccaga ttcccacggg agccttatta ctcgtttcag atcagccaat
      661 gatttcaacc ggcgtgaaaa ctacggaaag cgacaaatcg gttaccgcaa aatatgttga
      721 tgagcatgta gaaattggaa ttagggccat ggaaatgctt cgcgataacg gcagctcggt
      781 taaacactta aaatttttcg atgaatgaca catactgaga ttattgaaaa cctgaaaaag
      841 aagatttatc gccctgtata tgtcctcatg ggcgaagaac cttattttat cgaccttatt
      901 tcagattata ttgccgacaa cgtgctgccc gaaagcgaga aaagcttcaa ccaaaccatt
      961 ctttacggga aagatacaac tagcgacatg ctaattacca ttgcaaggag atttccaatg
     1021 atgtcggctt atcaggttgt cattctcaaa gaagcacaat tatttgataa acttgaggat
     1081 gaactgcttt cttatataga aaacccgctg aattcgacca tattggtaat ctgctacaaa
     1141 tacaaaaccc tcgataaaag aaagaaattt ctgaaagtgg cctcagaaaa aggaattgtt
     1201 ttcgaatcac caaaaatata cgaaaaccag ctgggtgcct ggatcagcaa atatattggc
     1261 gacaatggtt ttaaaattgc cccacaggca aatgccatgc ttgctgaata ccttgggacg
     1321 caaataagca aagtagccaa cgagttagat aaactaatga tattgttgcc caagggagcc
     1381 acaattactc ctgccgatgt tgagaaaaat attgggatca gcaaagagta taacattttt
     1441 gaattgcaga atgctctggg tgaacgcgac ataattaaat caaaccgcat tgtaaattat
     1501 tttgcttcga acaaacaaac cccattccct ttagttattg gaggcttagg gcaatatttt
     1561 gccaagctgc ttaaatacca ctttttgtcc gacaaatcga aaaacaatgc cgcaaaagca
     1621 cttggagtat caccctattt tgttgacaca tatattaaag cagcaaaata ctattcacct
     1681 aaaaagttgg tagaaattat atcaatgctg cgcgaattcg atatgaaatc gaaaggcatc
     1741 ggcaacgttt catcaactga aggagattta ctccgcgaac taacctataa aatcctgcac
     1801 tagaatgctc ctacttataa tactactcac ttctgtgatt tctgtaatgg ccttttctaa
     1861 tgcaaaaatt agcgaaaccc ttagcctgaa accagtgctt gtataccata ataaacaagt
     1921 ataccgagct attacccatt cgcttattca tgccgactgg gcacaccttc tggtcaacat
     1981 gtttgtacta ttttcgtttg ggcaagcttg tttggcctat ttcaatcagt tttttggcac
     2041 agcctccaat acctatttta taattcttta tataggaagc cttatttttt caagcctgtt
     2101 ttcaatatac aaacaccgca acaatccgta ttatacggct gttggagctt cgggagctgt
     2161 tatggcagta gtttttacct caatattttt cgacccctgg aatatgcttt atttctttgg
     2221 gataattccg atacccggaa tagtttttgg ttttttatac atgggttaca gtatttatat
     2281 ggggcgcaaa ggcaacgata atataggaca cgacgcccac ttttttggcg cacttttcgg
     2341 tttcttatac ccgctt
//
LOCUS       DAOPUW010000291         2354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_47973_length_2354_cov_2.895607, whole genome shotgun sequence.
ACCESSION   DAOPUW010000291 DAOPUW010000000
VERSION     DAOPUW010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47973_length_2354_cov_2.895607"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="PKJ43_05740"
     CDS             <1..1152
                     /locus_tag="PKJ43_05740"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ72104.1"
                     /translation="YLQRLRRIKQLGLSSLVFPGANHTRFEHTIGALHLMRQAIDVLR
                     EKKVSISTDEAEAVSIAILLHDIGHGPFSHALESAIVSGIGHEDLSLMLMEELNREFG
                     GKLTMAIEIFKDEYPRHFLHQLISGQLDMDRLDYLRRDSFYSGVVEGMIGCDRIIKML
                     NVADDRLVVDKKAIYSVENFLIARRLMYWQVYLHKTVLAAEFMLINILKRAKELVSNG
                     EELFAPPQLLWLLKHGFSKENLKINDNKSIMIKNFVALDDSDILSSIKVWSTHPDSVL
                     SWLSTAIINRKLFKIKISDHQFSEKKAEALKEQLHTKFNISKEEAGYFVFQGEITNSA
                     YEPSSEEIFIIYNNGKRADFEKASDINLSVLSKTVKKHFFCYPKELTLK"
     gene            1190..2224
                     /gene="lpxD"
                     /locus_tag="PKJ43_05745"
     CDS             1190..2224
                     /gene="lpxD"
                     /locus_tag="PKJ43_05745"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010421742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNZ72105.1"
                     /translation="MEFSALEIATFLNGEIIGDPTIKINNVSKIEDGKPGTLTFLANL
                     KYENFLYETKASVVLINADFVPVREVKATMIKVKDAYAALSSLLDLYVKSVPEKKGIE
                     QPNFIDSSAKIDEASYLGAFCYIGKNVSLGKNVKVYPHAWIDDNCIIGDNTILYTGVK
                     LYASTQIGKNCIVHAGAVIGADGFGFAPLEDGSYKKVQQIGNVTIEDNVEIGANSTID
                     RATLGTTRVKKGVKIDNLVQVAHNVVIGENTVMAAQFGVAGSTTIGQNCVFGGQAGVA
                     GHLKVGDNVTMAAKTGATNNIKDNTMLMGAWGIEASRYRRSEAVFRNLPELAAEVNKL
                     KKEIKNIKNE"
BASE COUNT          755 a          475 c          501 g          623 t
ORIGIN      
        1 tatttacagc gattacgacg aattaaacaa cttggtcttt cgagcctggt gtttccggga
       61 gcaaaccata cccgtttcga acataccatt ggggcccttc acctcatgcg gcaggctatt
      121 gatgtgctga gagagaaaaa agtcagcatt tcgaccgatg aagccgaagc tgtgagcatt
      181 gctattcttt tacacgacat cgggcacggg cctttttccc atgccctcga atctgccatt
      241 gtctcaggca ttggccatga agatttgtcg ctcatgctca tggaagaact gaacagggaa
      301 tttgggggca aactcaccat ggccatcgaa attttcaagg acgaatatcc gagacatttc
      361 cttcaccaac tcatttcggg acaactcgac atggaccggc tcgattattt aaggcgcgac
      421 agcttttata gcggggtggt tgaagggatg attggttgtg accggattat taaaatgcta
      481 aacgttgccg acgaccggtt agtggtcgac aaaaaagcca tctattcggt agaaaacttt
      541 ctgattgccc gccggttgat gtattggcag gtttacctgc acaaaaccgt tttggcggcc
      601 gaattcatgc ttataaatat cttaaaaaga gctaaagaat tagtttccaa tggggaagaa
      661 ttatttgccc ctccgcaatt actttggctg ctgaaacatg gtttttcgaa agaaaatctg
      721 aaaataaacg acaataaatc gattatgatc aaaaattttg tggcgctcga cgattcagat
      781 atcctttcct caataaaagt atggagtact cacccagatt cggttctttc ctggctttca
      841 acggcaataa taaaccgcaa gctttttaaa ataaagattt ctgatcatca gttctctgaa
      901 aaaaaggcag aagcacttaa ggaacagttg catacaaaat tcaacatctc taaagaagaa
      961 gccggttatt ttgtttttca aggcgaaata accaatagtg catacgagcc cagttcagaa
     1021 gaaattttca tcatttacaa caacggaaaa cgggccgatt tcgagaaagc ttctgacata
     1081 aacctgtcgg ttttatcgaa aacagtgaaa aaacatttct tttgctatcc gaaggaatta
     1141 acgcttaaat aaatttttta cttattttgc agcaaattta tttcaggtta tggaattttc
     1201 tgcactcgaa attgcaacgt ttttaaacgg tgaaatcatt ggcgatccta ccattaaaat
     1261 caataatgtt tcaaaaattg aagatggcaa accaggaacg ttaacctttc tggcaaactt
     1321 aaaatacgag aacttcttat acgaaacaaa agcttcggta gtattgataa atgccgattt
     1381 tgttccggtt cgcgaggtga aagccacaat gattaaagtg aaagatgcct atgctgcact
     1441 gtcatcgctg cttgaccttt atgtgaaatc ggtacccgaa aaaaagggca ttgagcaacc
     1501 aaattttatc gactcgtctg caaaaattga tgaggcctct tatctgggag cattttgcta
     1561 cataggcaaa aatgtaagcc ttggaaaaaa cgtaaaagtg taccctcatg cctggattga
     1621 cgataattgc attattggcg acaacactat tttatacacc ggggtaaaac tctacgcctc
     1681 aacacagatt ggaaagaatt gcatagtcca tgccggggct gtaatcggtg ccgacggttt
     1741 tggctttgcc ccattagagg acggtagtta caaaaaagta cagcaaattg gcaatgtaac
     1801 cattgaagat aatgtagaaa ttggcgccaa tagtaccatc gatcgggcaa ccttaggcac
     1861 aacccgtgtt aaaaaaggtg taaaaattga taatctggtt caggtggccc acaacgttgt
     1921 aataggtgaa aataccgtaa tggctgctca atttggagta gcaggttcca ccactatcgg
     1981 gcagaattgt gtctttggcg gacaagcagg tgttgccggc cacttaaaag ttggcgacaa
     2041 tgttaccatg gccgcaaaaa ccggtgcaac aaacaacata aaagacaaca ccatgcttat
     2101 gggtgcatgg ggaattgaag catcacgtta caggcgttca gaagcggtgt tcagaaacct
     2161 gcccgaactg gctgccgaag tgaacaagtt aaaaaaagag attaaaaata ttaaaaacga
     2221 ataacatatt aacagaatcg ttataatgac tgcaaaacag aaaaccattc ttcacgaagt
     2281 taaaatatcg gggaaagggc ttcacactgg gctcattgtt aatgcattat ttaaaccagc
     2341 tcccgaaaac cacg
//
LOCUS       DAOPUW010000292         2345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_48258_length_2345_cov_2.492576, whole genome shotgun sequence.
ACCESSION   DAOPUW010000292 DAOPUW010000000
VERSION     DAOPUW010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48258_length_2345_cov_2.492576"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..>2345
                     /gene="gcvP"
                     /locus_tag="PKJ43_05750"
     CDS             278..>2345
                     /gene="gcvP"
                     /locus_tag="PKJ43_05750"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNZ72106.1"
                     /translation="MIKNDKFGWRHIGPRSQEIDQMLKTIGVKSMDELIDQTVPKNIR
                     LEKPLDLGPGLSERQYYRKIHDLAQKNKVFNTYIGMGYYDVITPAVILRNVFENPVWY
                     TSYTPYQAEISQGRLEALLNYQTMVCDFTGMELANASLLDEATAAAKAMIMMFSLRSR
                     DQKKNGANVLFVDEKIWPQTLDVILTRAEPLGIEVKVDNYKNFGFNDKVFGVIVQYPN
                     SEGNIEDYSALVETAHKNECKVAVAADLLSLALLVPPGEWGADICFGSSQRFGVPMGY
                     GGPHAAFFATKDEYKRSMPGRIIGVSKDANGNRALRMALQTREQHIKREKATSNICTA
                     QALLATMAGFYAVYHGPRGIYSIAARIYSIANLLSKEIEKLGYKQQNEFFFDTVRFSL
                     PRHVKREDIEWLSLELKMNFRYFDNGEVGLSIDETTNVEDINWIVEVFAKAANKPVPT
                     FSDYPEECLINEKFARKSEYLQLDVFNRYRSETEMMRYIKKLENRDISLTHSMISLGS
                     CTMKLNAATEMLPLSWIEFSGIHPYVPKNQAMGYQIMMEELKSDLAQITGFADVSLMP
                     NSGAAGEYAGLMVIMAYHKSRGDEHRKVCLIPSSAHGTNPASAAMAGMEVVVTPCDDR
                     GNIDVEALKQKAKEHKDDLACLMITYPSTHGVFEASVAEVCKIIHQFGGQVYMDGANM
                     NAQVGLT"
BASE COUNT          701 a          553 c          496 g          595 t
ORIGIN      
        1 ccaacgacgt aattgtatat cccggccacg gaccggcaac aaccatcggc gacgaaaaag
       61 ccctcaatcc atttttacag taggacaaac aagatcaaat tatgattatg aaagcatttt
      121 ctattttttt gacaatactt cactctataa tatttttgta acaaacataa aacacttctt
      181 attgcgtcag gataactatc tcctatccaa aatcattttt tcgcagtgca aatcacctat
      241 ctttgcacca taaattcaca ttaaaatttc tattgccgtg attaagaacg ataaattcgg
      301 gtggcgccac atcgggccac gttctcaaga gattgaccaa atgttgaaaa ccatcggtgt
      361 taaatcgatg gatgaactga ttgatcaaac cgtaccaaaa aatatccgtc tcgaaaaacc
      421 gctcgatctt ggccccgggt tatcggaacg ccagtactac cgaaaaattc acgacctggc
      481 ccaaaagaac aaagtgttca acacatacat tggcatgggc tattacgacg taattacacc
      541 ggcagtaatc cttcgcaatg tgttcgaaaa tcccgtttgg tacacatcgt acacaccata
      601 tcaggccgaa atttcgcaag gccgccttga agccctgctc aattaccaga ccatggtttg
      661 cgattttacc ggcatggaac ttgcaaatgc atcgttgctc gacgaggcca ctgccgcagc
      721 caaagcaatg atcatgatgt tctcgttacg ctcaagagat caaaagaaaa atggtgcaaa
      781 cgttcttttt gttgatgaaa aaatatggcc acaaaccctc gatgttattc tcacccgtgc
      841 cgaaccgctt ggtattgagg ttaaagtaga taactataag aatttcggtt ttaacgataa
      901 ggtttttggg gtaattgttc aatatccaaa ttccgaaggg aatatcgaag attattcagc
      961 ccttgttgaa actgcccaca aaaacgaatg caaagtagct gttgccgccg accttctaag
     1021 ccttgccctg cttgtgcctc ccggtgaatg gggtgccgat atctgtttcg gtagcagtca
     1081 gcgttttggc gttccaatgg gctatggtgg ccctcatgct gcctttttcg ccacaaaaga
     1141 tgaatacaaa cgcagcatgc ccggccgcat tattggcgtg tcgaaagatg ccaacggaaa
     1201 ccgtgccctg cgcatggcgc tgcaaacccg cgaacaacat attaaaagag aaaaagcaac
     1261 ttcaaatatc tgtacggcac aagcattgct ggcaacaatg gccgggttct acgccgtata
     1321 ccacgggcct cgcggaattt attcaatagc tgcccgtatt tactccattg ccaatcttct
     1381 ttccaaagag attgaaaaat tggggtacaa acagcagaac gaattcttct tcgataccgt
     1441 acgctttagc cttccacgcc atgtgaaacg cgaagatatt gaatggcttt cgctcgaatt
     1501 gaagatgaat ttccgctatt tcgacaatgg cgaagtgggc ttaagtattg atgaaacaac
     1561 caatgtagaa gatatcaact ggattgttga agtgtttgca aaagcagcca acaaacctgt
     1621 tccaacgttt tccgactatc ctgaagaatg cttgatcaac gagaaatttg cccgcaaaag
     1681 cgaatacctt cagttagatg ttttcaaccg ttaccgttcg gaaaccgaaa tgatgcgtta
     1741 tatcaagaaa cttgaaaacc gcgatatttc gttaacccac tccatgattt cacttggctc
     1801 gtgcaccatg aaactgaacg cagccaccga aatgctccca ttaagctgga tcgaatttag
     1861 cggcatacac ccttatgtac caaaaaatca ggcaatggga taccagatca tgatggaaga
     1921 gttaaaatcg gacctagccc aaatcactgg ttttgccgat gttagcctga tgccaaattc
     1981 aggagccgct ggcgaatatg ccggactgat ggtgattatg gcttaccaca aaagccgtgg
     2041 cgacgaacat cgcaaagtct gcctcattcc ttcgtcggca cacggaacaa acccagccag
     2101 cgccgcaatg gccggcatgg aagtggttgt aactccttgc gacgatcggg gcaatatcga
     2161 cgtagaagcg ttgaaacaaa aagcaaaaga acacaaagac gatttggcgt gtctgatgat
     2221 tacttaccca tcgacccacg gtgttttcga agcatcggtt gccgaagtct gtaaaattat
     2281 acatcaattt ggtggtcagg tttatatgga tggtgccaat atgaatgccc aggtgggatt
     2341 aactt
//
LOCUS       DAOPUW010000293         2325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_48844_length_2325_cov_2.855066, whole genome shotgun sequence.
ACCESSION   DAOPUW010000293 DAOPUW010000000
VERSION     DAOPUW010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48844_length_2325_cov_2.855066"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PKJ43_05755"
     CDS             <1..484
                     /locus_tag="PKJ43_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72107.1"
                     /translation="GVWRINAEAISHTGYIIIIFQLLVSRIFEKQKALVSFVIGIVLI
                     SIGFIILGLSVLTVGSLVFLGVFVFAVGELVSSPRIQEYILWIAPKEKAGLYMGANFL
                     ATFIGAAFSGVYTWLMGRFEAAGNPQNIMFTLAAHTLLGIVAIYFFTKKLGSFKERVE
                     "
     gene            595..1188
                     /locus_tag="PKJ43_05760"
     CDS             595..1188
                     /locus_tag="PKJ43_05760"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNZ72108.1"
                     /translation="MKKLPIILSTLALIGVIGLLVMFLISNPSKNKSNGESAIAGSGG
                     LKIAYILTDSVLLNYQLAIDLHKDFLSQQQQYNSEFSRKRQNLESQATAFQEKLQRGG
                     FLTEDRAVKERDKILGMEQEIQQLDYELSSKLSQLEASINQRLSDSILNYVKEYNKDH
                     NYTFIFSNTGNIIVGAPQNNISRQILDGLNARYKSGK"
     gene            1244..>2325
                     /locus_tag="PKJ43_05765"
     CDS             1244..>2325
                     /locus_tag="PKJ43_05765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ72109.1"
                     /translation="MTEDFANSYFKKYGNALTQFPQQFFPLDLIVVIPCFNEPDIGRT
                     IHSLEQCEINSLRVACVVVVNSSEVSPPEVIEQNRKTMAHLTAAGSLLENKIKLFVVQ
                     SEKLPKKHAGVGWARKIGMDWAVAHFSYSGNRNGVIVSLDADTFVEENYLTAIWSYFQ
                     QYSKSVAATIAFEHRIETKDSNDQIVNDAIVWYELYMRYYRNALRYVGFPASFYTVGS
                     CFAVRADAYVAQGGMNRRQAGEDFYFLHKIATFGEVGEINSTTVFPSDRISDRVPFGT
                     GPVIRKFIEGDRSFCYSYALESFLVLNPFFMRVADFYSFNGDVPVSFFSANNLFQEFL
                     LANDLVTQIVELAANCGSCEMFTRRF"
BASE COUNT          645 a          421 c          492 g          767 t
ORIGIN      
        1 tggggtttgg cgtattaatg ccgaggcaat ttcacatacc ggctatataa ttattatttt
       61 ccaactcctt gtttccagaa ttttcgaaaa gcaaaaagca ctggtttctt ttgttatagg
      121 aattgtattg atttctattg ggtttattat ccttggattg tcggtgctta ctgtgggaag
      181 ccttgttttt cttggcgttt ttgtttttgc tgttggtgaa ttggtttctt ctccccgaat
      241 tcaggaatac atactttgga ttgccccaaa agaaaaggcc gggctttata tgggagctaa
      301 ttttttggct accttcatcg gcgcagcgtt tagtggagtt tacacttggt tgatgggacg
      361 gttcgaagct gcaggcaatc cccagaatat catgtttacc cttgctgcgc acaccttgct
      421 tggtattgtt gctatttatt ttttcacaaa aaaattgggc agtttcaagg aaagggtaga
      481 atgagtaatt gtctcttaca acttttgaac gaaaaaagag gcatttacat tttttatgtt
      541 ttgcctatgc gataaagaat tatatttgcg tgtatcaaaa aaaagatcaa ataaatgaaa
      601 aaattaccaa ttatacttag taccctggca ttgatcgggg taattggttt gcttgttatg
      661 ttcctgatta gcaatccatc gaagaataag agcaatggcg aatcagcaat agcaggctcg
      721 ggcggtttaa agatcgctta tattcttacc gattctgttc tcctgaatta tcaattggct
      781 atcgatttgc acaaagattt ccttagccaa caacaacaat ataatagcga gtttagccgt
      841 aaacgtcaga atcttgaatc gcaagcaact gcatttcagg aaaaattaca gcgtggtggc
      901 ttcttaaccg aagatcgtgc ggtgaaagag cgtgataaaa tcctcggtat ggaacaagaa
      961 atccagcagc ttgattatga gttgtcttcg aagttaagtc aattagaggc atctattaat
     1021 caacgccttt ctgatagtat tcttaactat gtgaaagaat ataacaagga tcacaactat
     1081 acgttcattt tcagcaacac cggaaacatt attgttggtg ccccgcaaaa taacatcagc
     1141 cgtcaaattc tcgatggcct taatgcccgt tacaaatcag gtaaataata gtaaaaatca
     1201 tatctttgag accgtgctca ttatctgaac acggtctttt tttatgactg aagattttgc
     1261 aaatagttat ttcaagaaat acggaaatgc tttaacccaa tttcctcaac aattctttcc
     1321 tctcgattta attgtcgtta ttccttgttt caatgaacct gatattggca gaaccattca
     1381 ttcgcttgaa caatgtgaaa taaattcgtt gcgtgttgcc tgtgttgttg tggtcaatag
     1441 tagtgaagtt tcaccaccag aagtgattga acaaaacaga aaaactatgg ctcatctgac
     1501 tgccgcaggt tctttgttgg aaaataagat taaactattt gttgttcagt cagaaaaatt
     1561 accaaaaaag catgccgggg ttggctgggc acgaaaaata ggcatggatt gggcagtggc
     1621 tcattttagc tactcgggca atcggaatgg ggtaattgtt tctcttgatg ccgatacctt
     1681 tgttgaggaa aattacctga cggctatctg gtcatatttt cagcagtata gcaaaagtgt
     1741 cgctgctact atcgccttcg aacatcgaat tgaaacaaaa gattcaaacg accagattgt
     1801 taatgatgca atagtttggt acgagctgta tatgcgctat tatcgcaatg cgcttcgcta
     1861 tgttgggttt cctgcctcat tttatactgt tggttcatgt ttcgcggtga gagctgatgc
     1921 ttatgtagct caagggggca tgaaccggag acaggcaggc gaagattttt attttcttca
     1981 taaaattgcc acgtttggcg aagttggaga aataaactcg acaacggttt tcccctcgga
     2041 ccgcatttcc gatagggtac ctttcggaac agggccagtt atcagaaagt ttattgaagg
     2101 ggaccgttct ttctgttatt cttatgcatt ggaatcattt ctggttctga accctttttt
     2161 catgagagta gccgattttt attcctttaa cggcgatgtg ccagtttcgt ttttttctgc
     2221 caataatctg tttcaggaat ttttattggc aaatgatttg gttacccaaa ttgtagagct
     2281 agcagccaat tgtggttctt gtgagatgtt caccagacgt ttttt
//
LOCUS       DAOPUW010000294         2323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_48909_length_2323_cov_3.477513, whole genome shotgun sequence.
ACCESSION   DAOPUW010000294 DAOPUW010000000
VERSION     DAOPUW010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48909_length_2323_cov_3.477513"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PKJ43_05770"
     CDS             <1..570
                     /locus_tag="PKJ43_05770"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNZ72110.1"
                     /translation="VDVKLSTLSFMTFGLSAGIETKRIDMERAVYLGAPFAADEYNAN
                     LFYTAIGINLFAQNLHVGGAFHLTPLKGTYYKSNESFSLYFNSSYLFTINDDWAVKPA
                     LVYRHFAGYNDLDIGAFVLYKDIVWIGLANRINSAMIFFADFKVTDFLRVGYSYDLSV
                     GKTSNIQYGTHEIGLEFTLPHKKTAQFQR"
     gene            632..2173
                     /locus_tag="PKJ43_05775"
     CDS             632..2173
                     /locus_tag="PKJ43_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNZ72111.1"
                     /translation="MKKIILIVLIAMTLTSVGQSVYIEKKDITPDGISSFGGVSYYNN
                     QLYLLLSATVRLDGKVVEASEAKSISQKYKVWTIFRYDPELRELAKMDQIWDTKNASA
                     NGFTIINDSTVVYSDVKSQLVSNDSYISSLLKKVNDVKFHYTDPFWDANRKRLYYASD
                     FQGGRGGMDIWYIETEGIYAGIPVNAGEVNSSSNELSPAFRSDSMMFYVSNAKNQQYD
                     IYMLDSEGFTVPESTRGVNEFFIAPKDKKNFFYVTSSGNKQSLVEGVLHIRKIENIEV
                     IAPVVVEEVKKEVEPEPEIVTPAEVSVEDTDFRIDNYFGVAKFTLTPMMQDSLNKIAD
                     MLQKDPSLSIVICGHSSPDGPEDLNMKLSSLRANEAYKWLQGKGVATNRIFRVYGGEY
                     LYNNMLFSRMFSIFPVSESEMPSQMVAVPASVLGNANTMYNLFGTDGDESEYWRFVLR
                     DKLPVDDKNILLLPVSEIHYVKVGETLFSLAKQYGITVEKMKQVNGLKTDNVPVGKIL
                     YIPEN"
     gene            2239..2322
                     /locus_tag="PKJ43_05780"
     tRNA            2239..2322
                     /locus_tag="PKJ43_05780"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2273..2275,aa:Leu,seq:cag)
BASE COUNT          670 a          438 c          546 g          669 t
ORIGIN      
        1 gtcgatgtaa aattgtcgac gttatcgttc atgactttcg gcttaagcgc cggtatcgag
       61 accaaaagga tcgacatgga acgtgcggtt tacctgggag cgccctttgc tgccgatgaa
      121 tacaacgcga acctttttta tacggctatt gggattaatc tctttgccca aaacctgcat
      181 gttggcggtg cttttcattt aaccccattg aagggaacct attacaaaag caacgagtct
      241 ttttcgctgt atttcaattc gagttacctg tttactatta atgatgactg ggcggtgaaa
      301 cctgccttgg tttatcgtca ttttgccggg tataacgatc tcgatattgg ggcctttgtt
      361 ttgtacaaag acatcgtttg gattgggctt gcaaaccgca tcaacagtgc aatgatcttt
      421 tttgccgact ttaaagtcac tgactttttg agagtgggat atagttatga tttgagtgtt
      481 ggaaaaactt cgaatataca atatgggacc catgagattg gcctcgaatt tacgttgcca
      541 cataaaaaaa cagcacaatt tcagcgctaa ttcttatatc gtggcgataa acaataaaaa
      601 tacattcaac gcctaaaatg ataaattacc aatgaaaaaa ataatcctta ttgtattgat
      661 tgctatgacg ctaaccagcg ttggacaatc ggtatatatc gaaaagaaag atattacgcc
      721 tgacgggatc agttcgttcg gcggggtgtc gtactataac aaccagcttt acttgttact
      781 gtcggcaact gttcgcttag atggtaaagt ggttgaagcc tcagaggcta aatcgatttc
      841 gcagaaatac aaagtatgga ctattttccg ttacgaccct gaattgcgtg agttggcaaa
      901 gatggatcag atttgggata caaaaaatgc ttcggccaac gggtttacca taatcaatga
      961 ttcaacagtg gtttacagcg atgtaaaatc gcagcttgta agtaacgatt catacatttc
     1021 ctcgttgctc aaaaaagtga atgatgtgaa atttcattat accgatccct tttgggatgc
     1081 caaccgtaag cggctgtatt atgcttccga ttttcaggga ggcagaggcg gaatggacat
     1141 ttggtacatc gaaacagaag ggatttatgc cgggattcca gtcaatgcag gggaggtaaa
     1201 cagctcgtcg aatgagcttt caccagcttt ccgcagcgat tcaatgatgt tttatgtgtc
     1261 gaatgcaaag aatcagcaat acgatatcta catgctcgat tctgaagggt tcaccgtgcc
     1321 ggaatcaacc cgcggggtta atgaattttt tattgcccca aaagacaaga agaatttttt
     1381 ctatgtgact tcatcgggaa acaaacagtc gcttgttgag ggagttttac atattcgtaa
     1441 gattgaaaat attgaagtga tagccccggt tgtggtcgag gaagtgaaga aagaagtgga
     1501 gcctgagccc gaaattgtta ccccggcaga agtgagtgtt gaagacaccg atttcaggat
     1561 cgacaattat tttggggtgg ccaaatttac cctcaccccg atgatgcagg attcgctgaa
     1621 caaaattgcc gatatgcttc aaaaagatcc atcgttgagt atcgtaattt gcgggcattc
     1681 ttcgcccgac ggacctgaag accttaatat gaaactttca tcactccgcg cgaatgaagc
     1741 gtataagtgg ctgcagggca aaggagtggc tacaaaccgc atatttaggg tgtatggtgg
     1801 cgaatacctt tacaacaata tgctgttttc gcgtatgttc tctattttcc cggtgagcga
     1861 atctgaaatg ccatcgcaaa tggtggctgt tcctgcttcg gttcttggaa atgctaatac
     1921 gatgtacaac ttgtttggta ccgacggcga tgaatcggaa tactggcgtt ttgtgctacg
     1981 ggataaatta cctgtcgacg ataaaaatat tttgttgtta cccgtaagcg aaatccatta
     2041 tgtaaaagta ggggagaccc tttttagttt ggctaaacag tatgggataa cagtcgaaaa
     2101 aatgaagcag gtgaatggat taaagactga taatgtacct gttgggaaga tattgtatat
     2161 ccctgaaaac taaattgagg gaaaaaagta taaattcatt tccgatacac tgaaaatttt
     2221 tatatttgca cccgcaatgc ccaaatggcg gaattggtag acgcgctagt ttcagggact
     2281 agtgttcgca aggatgtgca ggttcgagtc ctgttttggg cac
//
LOCUS       DAOPUW010000295         2317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_49087_length_2317_cov_2.929266, whole genome shotgun sequence.
ACCESSION   DAOPUW010000295 DAOPUW010000000
VERSION     DAOPUW010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49087_length_2317_cov_2.929266"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1956)
                     /locus_tag="PKJ43_05785"
     CDS             complement(<1..1956)
                     /locus_tag="PKJ43_05785"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNZ72112.1"
                     /translation="MKIKTFIIATTAIFALSQAKADEGMWLPAFLSQVNIEKMQKMGL
                     ELTATDIYNINNASLKDAVVVLDGGSCTGELVSPEGLFLTNHHCGYGEIQSHSTPENN
                     ILKNGFWAASYKEELANPGKTVSFLIRVEDVTETINASLNIDMDEDVRLDSIEAVSSR
                     LEAKAIEGNHYEAVVKDLYESNRFYLFVTETFRDVRLVATPPHFIGKFGGDTDNWMWP
                     RHTGDFSIFRIYTAPDGSPADYSADNIPLKPRHYLPVSIKGYKNGDFAMVMGYPGSTN
                     RYLTSDGVNYTMNVVNDSRITARKEKLDIMGNYMATSDEANIKYASKYARSSNYYKYS
                     IGQNRGLKRLDVVEKKKATEDKFNQWVNADESRKARYGEVSALLKDGYQNLEDDEARQ
                     YLIETLLRGPEIFAFTEKFMTLNTMLKANNKEQKNEEEISSLKEIASNYFKDYDQETD
                     KKITAALARIYAKEAAPKYHPLFFKTIEDKYKGNFEKWAEDLFKKSIFDNEDRVSAFL
                     ENPKSKVLANDPALVATQQLYDVLRKIRKENESDSKKLNKGYRLYLAGLMEMEPDKQF
                     YPNANSTMRLTYGKVGDYSPADAVHYNYYTTLDGYIEKEIPGDREFDVWPKLKEMYEA
                     KDYGQYADNDGTLHTCFITNNDITGGNS"
BASE COUNT          541 a          455 c          514 g          807 t
ORIGIN      
        1 agagtttcct cctgtaatgt cgttattcgt gatgaaacat gtgtgcaatg ttccgtcgtt
       61 gtctgcatat tgtccgtaat ctttagcttc gtacatttct tttagttttg gccaaacatc
      121 gaattcgcgg tctccgggta tttccttttc aatataccca tcaagggtgg tatagtaatt
      181 atagtgaaca gcatcggccg ggctataatc gccaaccttt ccgtaggtaa ggcgcatggt
      241 tgaattggca tttggataaa attgcttgtc gggctccatt tccatgaggc cagcaaggta
      301 aagccggtac cctttattga gtttttttga atccgattcg ttttcttttc tgattttcct
      361 caacacatcg tacaattgtt gcgtagctac aagcgctgga tcatttgcca atactttcga
      421 tttaggattt tcgagaaaag cagaaacacg gtcttcattg tcgaagatgc tttttttgaa
      481 aagatcttca gcccattttt caaagttgcc tttgtatttg tcttcaatgg ttttaaaaaa
      541 tagcggatgg tatttgggcg cagcttcttt tgcatatatc cttgctaacg ctgctgttat
      601 ctttttgtcg gtttcctgat cgtaatcctt aaaatagtta ctggctattt ctttgagaga
      661 acttatttct tcttcgtttt tttgttcttt attgttggct tttagcatag tattgagggt
      721 catgaatttt tcggtaaaag cgaatatttc agggccgcgc agcaaggttt caattaggta
      781 ttggcgcgct tcgtcgtctt caaggttttg gtagccgtct tttagcaacg ctgagacctc
      841 tccgtagcgg gcttttctgc tttcatcggc attaacccat tggttgaatt tatcttcggt
      901 cgctttcttt ttctctacca catcgagtct ttttaatccg cggttttgtc caatactata
      961 tttataataa tttgaactcc gggcatattt gctggcatat ttaatgttgg cttcatcgct
     1021 ggtggccata tagttgccca tgatgtcgag cttttctttt cgggcagtaa tgcgggaatc
     1081 gtttacaacg ttcatggtgt aattcactcc gtctgatgtg aggtatctgt tggtcgatcc
     1141 tgggtaaccc ataaccatgg caaagtcacc atttttgtat cctttaatcg ataccggcaa
     1201 ataatgtctg ggtttcagtg gaatattatc tgccgagtag tcggctggag atccatcggg
     1261 ggcagtataa atgcgaaata ttgaaaagtc gccggtgtgc cgtggccaca tccagttatc
     1321 ggtgtcacct ccaaatttac caatgaaatg cggaggggta gcaacgagcc ttacatcgcg
     1381 aaaggtttcc gtgacaaata gataaaaacg gttcgattca taaaggtctt ttacaacagc
     1441 ttcataatga ttgccttcaa tagcttttgc ctcaagcctc gacgaaacgg cttcgatgct
     1501 gtcgagtctt acgtcctcgt ccatatcaat atttaatgag gcgtttatag tttctgtaac
     1561 gtcttcaacc cgaatgagga atgaaactgt ttttcctggg tttgcgagct cttctttgta
     1621 gctagctgcc caaaatccat tttttaaaat gttattttcc ggggtcgaat ggctttgtat
     1681 ttcaccatag ccacaatggt ggttggtcag gaaaagccct tcgggcgaga ccaattctcc
     1741 ggtgcacgag cccccgtcga gtaccacaac agcgtctttg aggctggcat tgttgatgtt
     1801 gtaaatgtct gttgccgtga gctcaagccc cattttctgc attttttcaa tgttaacctg
     1861 cgacaggaaa gcgggtaacc acattccttc gtcggctttt gcctgcgaga gcgcaaatat
     1921 ggctgtcgta gcaataatga atgttttgat tttcataaaa ttgtcgtgta attgtatact
     1981 aaattctttg tcgttttctt ctttgtattg caatggtaaa aaaaaatagc gattagaaac
     2041 tgattctaac cggtaaaaat tgttgttttt attaaatgtt tctaaacaaa tgtattgctt
     2101 atttggttga tcgcgattga ttgtttattt gagaaagggc ggttagaggt tcaacgaaag
     2161 ttgctttggt gcaggcaata gctgaaatgt ttttcgatgg tgtggggtgt ctccatgctg
     2221 ggcaattgcc gcacgatgat cagcagtggg gtagcctttg ttctttttcc attgataata
     2281 agggtattca atgtcgatcc gtagcatgta atcgtct
//
LOCUS       DAOPUW010000296         2310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_49294_length_2310_cov_2.763636, whole genome shotgun sequence.
ACCESSION   DAOPUW010000296 DAOPUW010000000
VERSION     DAOPUW010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49294_length_2310_cov_2.763636"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PKJ43_05790"
     CDS             <1..752
                     /locus_tag="PKJ43_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72113.1"
                     /translation="SIYRWRNSDWKLLNSNIKDDLKNYTLSEENLSSNWRSRTNIVEF
                     NNWFFPISAAFLQDAYNQANISANPYQSTIVDVYADVQQIAASKKSGGIVQCELIDEE
                     KQPDYYSATTDRLISQIEKLQDAGVRASEMAILVKKNNQAMVVVDALLNYQKNNPLKP
                     YNFQVVSDDSLILAVALSVRFIVSMFRFVTSPDDVVNRANIVFLFHHYILPQLQKENI
                     LTVDNVENSGFSGKLISEDIALTYFPFFNNN"
     gene            810..>1068
                     /locus_tag="PKJ43_05795"
     CDS             810..>1068
                     /locus_tag="PKJ43_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNZ72114.1"
                     /translation="MIARYSLDKLPGEQASLQAFHDVVIDFSKKEGGNLAKFLEWWEL
                     KGSSLKVQTAVDRDAIRIMTIHKSKGLEFPYVFLPFCDWTFD"
     assembly_gap    1069..1168
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1169..1834
                     /locus_tag="PKJ43_05800"
     CDS             <1169..1834
                     /locus_tag="PKJ43_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HNZ72115.1"
                     /translation="AKLLTSTFSTDNASSLLRPLTESAFSYGTLECEKRETNPVNEIN
                     LSGKIDVKLNLSNSLKLHKNYDNFLEESEEKMSEKVNEGTLMHSILSLIETSGDISLA
                     VKQLVIEGKINVGESSFFVEKITALLLNEQVKCWFDGSLKILNETTIISPDFNLKRPD
                     RVMLGNDFAIVVDYKTTSLVSESHRKQVLGYLKKIRQMGIGKVEGYVWYLNSNELVYV
                     SEN"
     gene            1859..>2310
                     /locus_tag="PKJ43_05805"
     CDS             1859..>2310
                     /locus_tag="PKJ43_05805"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNZ72116.1"
                     /translation="MRKILNPYHDCSGDEYNCFGCSPNNGIGLHLHFFEDGEELYAEW
                     QPDANYMGYPGILHGGIQATLLDEIGGWVVYIKCETVGVTQDMTVKYLNPLRMDKGKV
                     SLRAKLIEQTEKNAIILATIFDGSGKLCSEATLNYFIYPQSIAVKRFY"
BASE COUNT          687 a          384 c          481 g          658 t
ORIGIN      
        1 aatcaatata ccgttggagg aacagtgatt ggaaattgct caatagtaac atcaaagacg
       61 atttgaaaaa ttacaccctt tcagaagaaa accttagcag caactggcga agccgtacaa
      121 atattgttga attcaacaat tggtttttcc ccatatctgc cgccttttta caagacgcat
      181 ataatcaggc gaatatttcc gcaaatccat atcagtctac cattgttgat gtgtatgccg
      241 acgtgcagca aattgcagca agcaaaaaaa gtggaggtat tgttcaatgt gaattgattg
      301 atgaggaaaa gcaacccgat tattatagtg ctacaaccga tcgcttgatt tctcaaattg
      361 aaaagttgca ggatgccggg gttagggcat ctgaaatggc tattctggta aaaaagaata
      421 accaggccat ggttgtagtc gacgcattgc tcaattatca gaagaataac ccattgaaac
      481 cctataattt ccaggttgta tctgacgatt ctctcattct tgcggtagct ctttctgtac
      541 gtttcattgt gtcaatgttc aggtttgtaa cttcgccaga cgacgtggtg aaccgggcca
      601 acatcgtttt tcttttccat cattatattc tgccgcaact gcagaaagaa aacattttga
      661 ctgttgataa tgtggaaaac agtggcttta gtggaaaatt gattagtgaa gacattgccc
      721 ttacttattt tccttttttc aacaataatt aatcaaaagt aattgccagg cattgggcct
      781 cgctttcgat aaccgatctt actgctgaat tgattgctcg ctattctttg gataagctgc
      841 ctggagaaca ggcaagcttg caggcatttc acgatgttgt tatcgatttc tctaaaaaag
      901 aagggggaaa tcttgccaag tttcttgagt ggtgggagct gaaaggaagt agcttaaaag
      961 tgcaaacggc tgtcgaccgg gatgcaattc gcattatgac tatccacaaa tcaaaaggct
     1021 tggaatttcc ttatgttttt ctcccttttt gcgattggac ttttgatgnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnngc aaaactgctt acttcaacat ttagtaccga
     1201 taacgcttcg tcactgttga gaccattaac tgaaagtgcg ttttcctacg gcacgcttga
     1261 gtgtgaaaaa cgggaaacaa atccggtcaa cgaaattaac ttgtcgggca agattgatgt
     1321 taagctgaac ctttctaata gcttgaagtt gcataagaat tatgataatt ttcttgaaga
     1381 atcggaagaa aagatgagtg aaaaagtaaa cgaaggcaca cttatgcata gtatactttc
     1441 attgatagaa acttctggtg atatatcatt agccgtgaag cagcttgtga ttgagggaaa
     1501 aatcaatgtt ggcgagtcgt cgttttttgt agaaaagatt acagctttgc tgctgaatga
     1561 gcaagtgaaa tgctggtttg atggctcgtt gaaaatctta aatgaaacca ctattatttc
     1621 gcccgacttc aacctgaagc gacccgaccg ggtaatgctg ggcaacgatt ttgccattgt
     1681 tgtcgattac aaaactactt cactggtttc agaatcgcac cggaagcaag ttctgggata
     1741 tttgaaaaaa atcaggcaga tgggaatcgg aaaagtagaa gggtacgtat ggtacctgaa
     1801 ttcgaatgaa ctagtatatg ttagtgaaaa ttaaaattaa ctcaaaagaa tattaagcgt
     1861 gagaaaaatt ttaaatccgt atcatgattg ttctggcgat gaatacaatt gctttggttg
     1921 ttctccgaac aatggaattg ggctgcacct tcattttttc gaagatggcg aagagttata
     1981 tgctgaatgg cagccagatg caaactacat gggatatccc ggtattttac atggaggaat
     2041 tcaggctact ttgctcgatg agattggagg ctgggttgtg tatataaaat gtgagacagt
     2101 tggcgtaact caggatatga cagttaagta cttgaaccca ttgcgaatgg acaaaggtaa
     2161 agtttcatta agagcaaaac tcattgaaca aacagaaaag aatgcaatta ttcttgctac
     2221 aatatttgat ggaagcggga aattatgttc cgaagccaca ttgaattatt ttatttatcc
     2281 tcaatcaatt gcggttaaaa ggttctatta
//
LOCUS       DAOPUW010000297         2309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_49321_length_2309_cov_2.962733, whole genome shotgun sequence.
ACCESSION   DAOPUW010000297 DAOPUW010000000
VERSION     DAOPUW010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49321_length_2309_cov_2.962733"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="PKJ43_05810"
     CDS             complement(<1..1200)
                     /locus_tag="PKJ43_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNZ72117.1"
                     /translation="MSDFYLILTVVLFALAISDLIVGVSNDAVNFLNSAIGSKSAPKW
                     LIFGIAGLGILIGSTFSNGMMEIARKGIFHPEMFVFSEIMVIFLAVMITDVIILDLFN
                     TFGFPTSTTVSIVFELLGAAVSVSLVKMAQVGEAFTELSKYINSEKALMIIVGILTSV
                     VISFIVGVLIHWITRFLFSFRYESRLKYLGGIYGSLAITFIIYFMIIKGAKDASFMTP
                     ENYEWISANMKSILIYCFIGLSILLQGLYWLFKIDILKVVVLAGTFALAWAFAGNDLV
                     NFIGVPLAGFAAYKSWVASGVPADSFNMISLQGEVKTPTIMLVIAGIIMVVTLISSKK
                     ARSVIKTSVDLSRQTEGEERFSSSAFSRLIVGVSMRTSDMVSRIVPVKVSNFIERQFQ
                     TVPEDKTI"
     gene            1430..>2309
                     /locus_tag="PKJ43_05815"
     CDS             1430..>2309
                     /locus_tag="PKJ43_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ72118.1"
                     /translation="MKSLRPRAIAVWTSVIVSLVLSVAAYFLFLVSGGSPLILIIFFV
                     ISFFLVFMGANIFLNHLIEKRLKPIFKIIRDVPVEEVRKAREYSRKRNLNIDVNDEVT
                     LWATQKQAEIQKLKELERYRKEFVGNVSHELKTPIFNIQGYILTLLDGGIDDPSINHL
                     YLQRTVKSIDRMISIVEDLESINKLESGELKLKMENFDFVRLVEDVFEIELLMAQERK
                     INLEFSTRTDKPVKVYADKKRIMEVVSNLVVNSIKYGKKGGYVRVGFFDLDDHIVVEV
                     SDNGIGVDKDHLPRIFE"
BASE COUNT          746 a          409 c          454 g          700 t
ORIGIN      
        1 aatggtttta tcttcaggaa cagtttgaaa ttggcgttca ataaaatttg aaacttttac
       61 aggaacaatt cgcgatacca tatcgcttgt tctcatgcta acccccacaa taagtctcga
      121 aaatgctgat gagctaaatc gttcttctcc ttccgtttgc ctcgaaagat caacagaagt
      181 ttttataaca gaacgagctt tttttgatga aattagagtt actaccatta tgatgcctgc
      241 aataacaagc attatggttg gtgttttaac ctccccctga agggatatca tgttgaacga
      301 atcggccggg actcctgatg ccacccagct tttatatgcg gcaaacccgg ctaatggaac
      361 accaataaaa tttactaaat cgttgccggc gaaagcccat gcaagggcaa atgttcctgc
      421 taaaacgaca acttttagta tatctatttt gaagagccaa tacaaccctt gtaacaaaat
      481 agataagcct ataaagcaat atataagaat cgatttcatg ttggctgaaa tccattcgta
      541 attttcgggt gtcataaatg aagcatcttt tgctccttta atgatcatga aatagatgat
      601 aaatgtgatg gccaaactac catatatccc acctaaatat tttagacgac tttcgtaacg
      661 aaatgagaat aagaatcttg tgatccagtg tattaatacc cctacaataa aagagatcac
      721 caccgaagtg agtatgccca ctatgatcat caatgctttc tctgagttga tgtatttact
      781 taattctgta aatgcttctc caacttgggc cattttaacc agcgatacag aaactgcagc
      841 tccaaggagt tcaaaaacaa ttgatactgt agtggaagtg ggaaacccga aagtgttgaa
      901 aagatcaagg ataattacat cggtgatcat aaccgcaagg aaaatgacca tgatctcaga
      961 aaacacgaac atctcggggt gaaaaattcc ttttcgggca atttccatca tgccgttcga
     1021 aaaagttgaa cctataagaa ttcccagacc tgcaatgcca aaaattaacc atttgggggc
     1081 cgattttgat ccaatggccg agttgagaaa gttaacagca tcgttcgaaa caccaacgat
     1141 taaatcagat atggcaagtg cgaacaatac aacggtaaga attaaataga agtcactcat
     1201 tatttacagg tgttatttat ggttttgccc attttaaagc ccgcacaaag ctacgtccaa
     1261 ttgtgaagcc aaaagttttt acttttttaa aaaaatgtta aggaatagta aagtcaagtt
     1321 gttcttggca attgttgttc aatgtgtttt gctgagagtt atggcttata ataaaattaa
     1381 ttgtcagtgt agtttttcat ttctaattta gcgaaaaaaa tgtcggttta tgaagagttt
     1441 acgccctcgg gctattgcgg tatggacttc ggtcattgtt tcattggttc tttcggttgc
     1501 agcttacttc ctttttctcg tgtcaggagg tagtccgctg atattgatta tattttttgt
     1561 gatatctttt ttccttgttt tcatgggtgc aaacattttt ctgaaccatc ttattgaaaa
     1621 gaggttaaaa ccaatattca aaattattag ggatgttcct gttgaagaag taagaaaagc
     1681 cagggaatat tcaagaaaac gaaacttgaa tatcgatgtg aacgatgaag taacattatg
     1741 ggcaacacaa aaacaggccg aaatccaaaa attaaaagag ttggaacgct atcggaagga
     1801 gtttgttgga aatgtttctc acgagctgaa aaccccgatt tttaatattc agggatatat
     1861 tctcaccctt ctcgatggag gcatagatga tccaagcatt aaccatttgt atctgcaacg
     1921 tactgttaaa agcatcgacc gaatgatatc gatcgttgaa gatcttgagt cgatcaataa
     1981 gcttgaatcg ggcgaactga aacttaaaat ggaaaatttc gattttgtcc gtttagttga
     2041 agacgtgttc gaaattgaat tattaatggc acaagagcgt aaaattaacc ttgaattctc
     2101 gactcgtacc gacaaacccg taaaggttta tgccgacaaa aagagaatta tggaagtagt
     2161 tagtaatctt gttgttaata gtattaaata cggaaaaaaa ggtggttatg ttcgcgttgg
     2221 gttcttcgac cttgatgacc atattgttgt agaggtttct gataacggga taggggtaga
     2281 taaagatcat ttgccccgta ttttcgagc
//
LOCUS       DAOPUW010000298         2300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_49592_length_2300_cov_3.337639, whole genome shotgun sequence.
ACCESSION   DAOPUW010000298 DAOPUW010000000
VERSION     DAOPUW010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49592_length_2300_cov_3.337639"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PKJ43_05820"
     CDS             <1..709
                     /locus_tag="PKJ43_05820"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNZ72119.1"
                     /translation="DLADVFSANEEEYLERKLVDFFASTSNQIAVVTVSDLEGYDISD
                     LAFRIGADWGIGSEKFDNGVLILLKPKTADSNGQAFIAVGYGLEGVVPDITAKQIVEN
                     EMIPRFKEGNMFAGIDNAANVLIELAKGEYNYQQYAKRSEGSPLVFFILIGLFIVLPI
                     LFGRRRSGFTVGSRNSSLPFWITMGLLNGLGNRRSGSFSDFSRGSGSFGGGGSSFGGF
                     GGFGGGGFGGGGAGGSW"
     gene            complement(770..1300)
                     /locus_tag="PKJ43_05825"
     CDS             complement(770..1300)
                     /locus_tag="PKJ43_05825"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNZ72120.1"
                     /translation="MEKKFTDFEELRKRIDLKCKSLHAINEKYMVCKKGCDECCMNFN
                     LLPIEFNAILEAIKPLDIKINSNSAPNACPFLVDHECQIYEHRPIICRSHGLPILTMD
                     EEGENWELSFCPLNFTNTDEDYFEEEKCYEQDVFNSELYMINQDHSTSTGIDPHEMSD
                     LHQLAERYSSERLKRK"
     gene            1503..>2300
                     /locus_tag="PKJ43_05830"
     CDS             1503..>2300
                     /locus_tag="PKJ43_05830"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72121.1"
                     /translation="MEKSLLILLIVIFAPFLLVAQTDESEGDINSEQNPTMDANVVAV
                     PVIPSLRAYRLENQYSLQKSYPADTSMLNFHVFSPMFRNSITNTYLGFTGAPYISDNF
                     FDRFEYPDYFFFNSIKAYVRQIKDVTFYNTNTPFSYLKYAEGSEDIEQAFTAFFTQNI
                     DSVSNFGFSFDVENGVGDYKNQMGKHKNLNLFYSQNTPRSTIYATIINGTNLLSENGG
                     INNKTINPYANTAMVGVQLSGKLTSEIKSLVAMASYEYRMGEIPFLKK"
BASE COUNT          634 a          417 c          478 g          771 t
ORIGIN      
        1 cgatttggca gatgtttttt cggcaaacga agaagaatat ttggaacgga agcttgtcga
       61 tttttttgcc agtacttcta accaaattgc ggtggtaaca gtcagcgatt tagaaggata
      121 tgatatttct gatttagctt ttcgtattgg tgccgattgg ggcattggca gcgaaaagtt
      181 tgataacggt gttcttatat tgttgaaacc gaaaactgct gatagtaatg gacaggcctt
      241 tattgccgta gggtatgggc tcgaaggtgt tgtccccgat attactgcta aacaaattgt
      301 tgaaaatgag atgatcccta ggttcaaaga aggtaacatg tttgccggta tcgacaatgc
      361 cgccaatgtg ttgattgaac tagcgaaagg agaatacaac taccagcaat atgcaaaaag
      421 gagtgaagga agtcccctgg tgttttttat actgattggc ttattcattg tgcttcccat
      481 attatttggc cgacgccgtt cgggtttcac agtgggttcc cgcaatagta gtttgccatt
      541 ctggattacc atgggactgc tcaacggatt gggaaacaga aggtctggat cgttcagcga
      601 tttcagtcgt ggttccggtt cgtttggcgg aggtggcagc agctttggcg gctttggcgg
      661 tttcggcggc ggcggtttcg gcggcggcgg tgccggtgga agctggtagc atgctctatt
      721 atttcgaata gatttataac taaatagaaa gcgctttcgg gcgctttttt catttccttt
      781 ttaatcgctc tgacgaatat ctttccgcaa gttgatgtaa atctgacatc tcatgaggat
      841 cgattcctgt tgaggttgaa tgatcttggt ttatcatata cagttcgctg ttgaatacat
      901 cttgttcgta acatttctcc tcttcaaaat aatcttcgtc ggtattggtg aaatttagcg
      961 ggcaaaacga aagctcccaa ttttcacctt cttcgtccat ggtgagtatt ggcaaaccat
     1021 gcgaacggca gataatcgga cggtgttcat aaatttggca ctcatggtca acaagaaacg
     1081 gacaagcatt gggggcagaa ttactgttga tttttatatc caatggctta attgcttcta
     1141 aaattgcatt gaactctata ggtagcaggt tgaaattcat acaacattcg tcgcagccct
     1201 ttttgcaaac catatacttt tcatttattg cgtgcaatga cttgcatttc aggtcaattc
     1261 tttttcttag ttcttcaaaa tcagtaaact ttttttccat aattatatat tgtatggttc
     1321 atatttctat tgggtgtaac ttttcaatag ggttgacttc gcaaaaatcg ctttcatctt
     1381 ttaaataatg aaatagtttt tacctatttt gcgttaacga aatatgttta aagaatcagg
     1441 ctttaataaa cattaatatt tattggcaca aagtttttca acataattct atctttgctt
     1501 cgatggaaaa atcattattg atactattga tcgtaatttt tgcccctttt ttattggttg
     1561 ctcaaactga tgaatcagaa ggggatataa atagcgaaca gaatccaacg atggacgcta
     1621 atgtggtggc tgtccctgtt attccctcat tgcgtgcgta tcggctcgaa aatcaatatt
     1681 cattgcaaaa aagttatcct gccgatactt cgatgttgaa ttttcatgtt ttttccccaa
     1741 tgtttcgaaa ttcgattacc aatacctatt tgggttttac cggggctccc tatatttctg
     1801 ataatttttt cgaccgtttc gagtatcccg attatttttt ctttaattct ataaaagcct
     1861 atgtcagaca aataaaggac gttacctttt acaatacgaa cactcctttt tcatatctga
     1921 aatatgcgga aggaagcgag gatattgaac aggcttttac tgcatttttc actcagaata
     1981 tcgattcggt ttcaaatttt ggtttttctt tcgatgttga aaatggggtt ggcgattaca
     2041 agaaccaaat ggggaaacat aaaaacctga acctctttta ttcccaaaat acaccacgtt
     2101 caacgatcta cgcaacaatt ataaacggaa caaacctact ctctgaaaat ggtgggatta
     2161 acaataaaac gattaatccc tatgcgaata ctgccatggt tggtgttcaa ttgtctggaa
     2221 aactgacatc agaaattaaa tctttggttg ccatggcttc atacgaatat cgtatgggag
     2281 aaattccttt tttgaaaaag
//
LOCUS       DAOPUW010000299         2297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_49690_length_2297_cov_2.692239, whole genome shotgun sequence.
ACCESSION   DAOPUW010000299 DAOPUW010000000
VERSION     DAOPUW010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49690_length_2297_cov_2.692239"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2117
                     /locus_tag="PKJ43_05835"
     CDS             <1..2117
                     /locus_tag="PKJ43_05835"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HNZ72122.1"
                     /translation="VKEWLSQLLADEYNVIRSPKSFNSQIGVPLSVWLLDNDADIAII
                     EAGISQPGEMELLQRIVKPDIGIFTNIGEAHQSNFESVEQKIEEKLKLFVGTDVIIYH
                     KDYQGITDAIGSLAEHKSLCPITWSMMDETADLFIYFVQKIPEGCGIIGQSGDKQYAI
                     SIPFSDDASVENACTCLVALIAIGKDQDHVLNRFSNLQPVAMRLEIKQGKNECIVIND
                     SYNSDLNSLTIALDTLDQQASQGFLSKTLILSDIFYSPKTDEVLYAEVAELVRLRMMD
                     RFIGVGPNISKQAHLFGDNAHFYESTLEFLNELPLLEFKQEAILLKGSRDFQFDLIAT
                     TLQYKAHETVLEIDLSAMVHNYNYFKSMLKPDVKIMAMVKAFSYGSGTAEIARVLEFH
                     HADYLAVAIADEGVTLRNAGIKMPIVVMNPEEHSFDLMIEHQLEPVIYSLRLLDQFIP
                     YAHRSAVNYFPVHIKFDTGMRRLGFDSEDELKKLLAKINKTNELFISSVFSHLAVSDD
                     PDEDAYTHMQIDRFEQLAAVVQAGCDHPVMRHILNSSGIERFPDNQYEMVRLGIGLYG
                     VSVVNKKALMNVSTLKTTVSQLRIVRPGETIGYGRKGVAKGDMVIATLPIGYADGFNR
                     HLGNGVGKVWINGQLAPVVGNICMDMCMVDVSDIDVKEGDRVIVFGKELPASEMAEAL
                     GTIPYEIFTSVSQRVKRIYFQE"
BASE COUNT          626 a          461 c          553 g          657 t
ORIGIN      
        1 ttgtaaaaga gtggcttagt cagttgttgg ccgacgaata caatgtaata agaagcccca
       61 aaagttttaa ttcgcaaatt ggcgttcccc tttcggtgtg gttgctcgac aatgatgccg
      121 acattgcaat aattgaagcc gggatatcgc aaccgggcga aatggaactg ttgcagcgca
      181 ttgtaaagcc cgatattggc atttttacca atatcggcga ggctcaccag tcgaattttg
      241 aatcggtgga acaaaagatt gaagaaaagc tgaaactgtt tgttggtacc gacgtaatta
      301 tttatcacaa agattaccag ggcattactg atgcaattgg tagtttagcc gagcacaagt
      361 cgctgtgccc cattacctgg tcgatgatgg atgaaaccgc cgaccttttt atctattttg
      421 tgcaaaagat tcccgagggt tgcggcatta taggccaatc gggcgataag caatacgcca
      481 tttctattcc attttccgac gatgcatcgg ttgagaatgc ctgcacctgt ttggttgccc
      541 ttattgccat tggcaaagat caggaccatg tgcttaaccg gttcagcaac ctgcaacctg
      601 tagctatgcg ccttgaaata aaacaaggga agaacgagtg tattgtgata aacgactcgt
      661 acaattcaga ccttaattcg ctgaccatcg ctctcgatac cctagaccag caggctagcc
      721 aaggcttcct ttccaaaaca cttatccttt ccgatatctt ttattcgcca aaaaccgatg
      781 aagtgttgta tgccgaagtg gctgagttgg tgcgtttgcg aatgatggat cgttttattg
      841 gtgttggccc taatatttcg aaacaggccc atttgtttgg cgataatgcg cacttctatg
      901 aatcgaccct tgaatttctt aacgaattgc cgttgcttga gtttaagcaa gaagccattt
      961 tgctgaaagg gtcgcgcgat tttcagtttg atctgattgc cactacatta caatataaag
     1021 ctcatgaaac cgtgctcgaa atcgatcttt cggcaatggt acacaattat aattacttca
     1081 agagcatgct caaacccgat gtaaaaatta tggctatggt caaagctttt tcatacggga
     1141 gcgggactgc cgaaattgcc cgggttctag agtttcatca tgccgattat ctggcagtgg
     1201 ccattgccga tgaaggggtt accctgcgca atgccgggat taaaatgccc attgtggtaa
     1261 tgaaccccga ggagcatagc tttgatttaa tgatagaaca tcaactcgag ccggtaattt
     1321 attcgcttcg tttgctcgac cagtttattc cgtatgcgca ccgaagtgct gtgaattatt
     1381 tccctgtaca tattaaattc gataccggga tgcgaaggtt aggtttcgat tcggaagatg
     1441 aactgaaaaa actgttggca aaaattaaca agactaacga gttgtttatc agttcggtat
     1501 tttcacatct agcagtgagc gacgatcccg atgaagatgc atatacccac atgcaaattg
     1561 atcgtttcga gcagttggcc gccgtggttc aggcgggttg cgatcatcct gttatgcgcc
     1621 acatacttaa ttcgtcgggc attgagcgtt tccccgacaa ccaatacgag atggttcgtt
     1681 tgggcatagg gctttatggg gtcagtgtgg tcaacaaaaa ggcattgatg aatgtgagca
     1741 cgctgaaaac cactgtttcg caattacgca ttgtgcggcc aggcgaaacc ataggctacg
     1801 gacggaaagg tgtggctaag ggtgatatgg taattgctac cctgccaata ggctatgccg
     1861 atgggtttaa ccggcatttg ggcaacggag tgggaaaagt gtggataaac ggacagcttg
     1921 ccccggtagt tgggaatatt tgcatggaca tgtgcatggt tgatgtaagt gatatcgacg
     1981 tgaaagaagg cgaccgggta atcgtttttg gcaaagaact accggcatcg gaaatggctg
     2041 aagctttggg aaccattcca tacgaaatat tcacttcggt ttcgcaacgg gttaaacgga
     2101 tatattttca ggagtaagaa taaattactt caataattta tttcaggatt taatttgaaa
     2161 atctgcaaaa gttcgaaggc ttttcggagc tagaaactat tgaattttga tgcttcaaag
     2221 gattattgcc aattgttatt attcggtttt acttcgtcgg gtcagtttcc tgtgattatc
     2281 agtatattct ttaagaa
//
LOCUS       DAOPUW010000300         2296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_49715_length_2296_cov_3.530120, whole genome shotgun sequence.
ACCESSION   DAOPUW010000300 DAOPUW010000000
VERSION     DAOPUW010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49715_length_2296_cov_3.530120"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="PKJ43_05840"
     CDS             complement(<1..1361)
                     /locus_tag="PKJ43_05840"
                     /inference="COORDINATES: protein motif:HMM:NF039640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6057 family protein"
                     /protein_id="HNZ72123.1"
                     /translation="MKNIIRYLLFVLSGFAVFYVMYEMVNTELIYHLQQLGFSSSKAY
                     FNEMMSFPGGPAEYLSVFLFQYAYNSFTGALLFSAILFVILSLTGFVLKGNRSALDGF
                     LIFIPAALTAVLLVEYDLHPVFPLLVLSLLIFMSFFTAIIHAKRNTGLQIFSIILLFA
                     AFYWLAGGLIFLVFSLSALIWSVRELRGKRLLITSLTVTILAGVLPLIAPKFFFIGQR
                     DAFLKLGPYFTDYKPGYLLYGVVFSLPFSLLMHSISRFKNIDFVGKIPFGRTKFLQNS
                     FSLIVMGGLLAGMYFHIDQQQKFKIQVDKLAHERKWNEVLTMVGERTINDRITQFHVN
                     RALFFTDSLTTSLFKYDQRWGVDGLFLTRQFSNDMLLPSTELFFDLAFVNEAIHYANE
                     ALAQNEHSPLLIEQLILANIASADSQAARLYVNVLKDYPTFQQRARMYEKHINGTTNP
                     DLE"
     gene            complement(1412..1798)
                     /locus_tag="PKJ43_05845"
     CDS             complement(1412..1798)
                     /locus_tag="PKJ43_05845"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HNZ72124.1"
                     /translation="MKYEAALLMMKKEKIFEAILVITTGFIVLFAVLGKEWMLFVAIA
                     SGLVGLLIPELAAVLAKVWFKIGEVLGFVVSKVVLFLLFYVLLTPIAFLYRLFNHDSL
                     QLKRQNRSLWSERNHIFQPTDLKNSW"
     gene            complement(1999..>2296)
                     /locus_tag="PKJ43_05850"
     CDS             complement(1999..>2296)
                     /locus_tag="PKJ43_05850"
                     /inference="COORDINATES: protein motif:HMM:NF028171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNZ72125.1"
                     /translation="PAITHIDMSARIQTVHRDTNPRYWQLINEFKKLTEVGLVVNTSF
                     NVRGEPIICSPDDAYRCFMRTEMDYLVINNFVFVKEQQPAWNEKDNWKEEFKPD"
BASE COUNT          802 a          466 c          429 g          599 t
ORIGIN      
        1 tcttcaagat cggggtttgt agtcccattt atatgtttct cgtacatacg ggctctttgc
       61 tgaaacgtag gatagtcttt cagcacattc acatacaaac gggcagcttg tgaatcggcc
      121 gaggcaatgt tagccaaaat aagctgttcg atcagaaggg gtgaatgttc gttttgagca
      181 agggcttcgt tggcataatg aatagcttcg ttaacaaagg ccaagtcgaa gaaaagctca
      241 gtagacggca gcaacatatc gttcgaaaat tgtcgggtaa ggaacaaacc atctaccccc
      301 caacgctgat cgtacttgaa aagactggtg gtaaggctgt cggtaaagaa aagagcccta
      361 tttacatgga attgggtaat tcgatcatta atagtgcgtt cgccaaccat ggtaagaacc
      421 tcgttccatt tacgttcatg tgccagttta tcaacctgaa ttttaaattt ttgttgttga
      481 tcgatatgaa aatacattcc tgcaagaagt cctcccatta cgataagcga aaatgaattt
      541 tgtaaaaatt ttgttcgccc aaaaggaatt ttacctacaa agtcgatatt tttgaatctt
      601 gatatagaat gcattaacag agaaaatggc aacgaaaaca caactccata aagcaaataa
      661 cccggtttat aatcagtaaa atatggcccc agctttaaga aagcatcccg ctgtccaatg
      721 aagaagaatt ttggggcaat taatggcaac acccctgcaa gtatggtaac tgttaaagag
      781 gtaatcaaca gtcgtttgcc tcgtagttca cgaaccgacc aaataagtgc agaaagcgaa
      841 aagacgagaa aaatcaaacc tcctgcaagc caataaaaag cagcaaatag cagaattatt
      901 gaaaatatct gaagtccggt attccgtttg gcgtggatta tagccgtaaa aaacgacata
      961 aaaataagca aggataacac cagtaaggga aagacagggt gaagatcgta ctcaacaaga
     1021 agcaccgcgg taagggcagc tggtataaaa ataagaaaac catcgagagc cgagcggttc
     1081 cctttcaaca caaaaccagt gagtgaaagg attacaaaca atattgcaga aaaaagcaat
     1141 gctcccgtga atgaattgta tgcatattga aatagaaata cagaaaggta ttcggccggc
     1201 ccacccggga aactcatcat ttcattaaaa taagctttcg acgacgagaa acccaattgc
     1261 tgcaaatgat aaatcaactc ggtattcacc atttcgtaca tgacgtaaaa aacagcaaag
     1321 cccgataaga caaataataa atagcggatt atatttttca tttcgcttga aaattattca
     1381 ttattgattt taagccattg gacaacaacc attaccagct atttttcaaa tctgttggtt
     1441 gaaagatgtg gttacgttcg ctccacaaag aacggttttg ccttttcaac tgtaagctat
     1501 catggttaaa caacctatat aaaaatgcaa taggtgtcag taaaacataa aataacaaaa
     1561 aaagaacaac cttcgaaact acaaatccaa gaacttctcc aattttaaac catactttgg
     1621 ctaaaacagc ggccaactct ggaataagta agccaacaag tcctgaagcg atggcaacaa
     1681 acagcatcca ttcttttccc aaaacggcaa aaagaacaat aaaacctgtg gtaatcacca
     1741 aaatagcttc aaatattttt tcttttttca tcatcaacaa tgctgcctca tatttcaaaa
     1801 aagcgtgtat ataaatgggg ccaacgcact ccctgagctc aacacaatta aaacacccaa
     1861 caacaggaag acaataagcg tgggaatgag ccaccacttt ttgcgttcct tcatataatt
     1921 ccaaaaatcg ctgagaaaat ccatatctta atgtttattt ttttgcgaat ttattgctaa
     1981 aagaacgaaa acggaaattt aatcgggttt aaattcctct ttccaattat ccttctcgtt
     2041 ccaggctggt tgttgttcct tcacaaaaac gaaattattg attaccaaat aatccatttc
     2101 ggttcgcata aaacagcgat acgcatcatc gggcgagcaa attatgggtt cgccccgaac
     2161 attgaaactc gtattaacca ccaaacctac ttctgtaagc tttttaaact cgttgattaa
     2221 ctgccagtat cggggattcg tatcgcgatg cacggtttgt atccgtgccg acatatcgat
     2281 atgtgtaatg gccggg
//
LOCUS       DAOPUW010000301         2281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_50207_length_2281_cov_3.498652, whole genome shotgun sequence.
ACCESSION   DAOPUW010000301 DAOPUW010000000
VERSION     DAOPUW010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50207_length_2281_cov_3.498652"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PKJ43_05855"
     CDS             complement(<1..228)
                     /locus_tag="PKJ43_05855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HNZ72126.1"
                     /translation="MDTNRVTVRYAKAFVELASEEDVLMECYHDISVLYYSLTNYQGF
                     RDSLVGQKMKANEKLGHLKEMFADSFQPLTLR"
     gene            complement(235..726)
                     /gene="atpF"
                     /locus_tag="PKJ43_05860"
     CDS             complement(235..726)
                     /gene="atpF"
                     /locus_tag="PKJ43_05860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNZ72127.1"
                     /translation="MFLIPHVGTIIWTTIIFLLLVYILGKFAWKPLLKALAEREQNIE
                     RALTGAAEANEKIKSLQAEQEEIIKATRLEKEQLLKEGVLQRNTIISDAKIKAQEEAD
                     KIIENTRKQLAFERAKALEDLRNQVAELSLEIAGKVVSAEMSDKIRHEKLVKELVDDI
                     ELN"
     gene            complement(783..1028)
                     /locus_tag="PKJ43_05865"
     CDS             complement(783..1028)
                     /locus_tag="PKJ43_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNZ72128.1"
                     /translation="MTLFIILAELALGKLSAGIGAGMAALAAGIGIGKIGASAMEAIA
                     RQPEANADIRSSMILASVFIEGVAFLAIILCILLAVL"
     gene            complement(1041..2096)
                     /gene="atpB"
                     /locus_tag="PKJ43_05870"
     CDS             complement(1041..2096)
                     /gene="atpB"
                     /locus_tag="PKJ43_05870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNZ72129.1"
                     /translation="MEWCRYCVFILLIAFCVPKASYAEEAEEHNDFNTVEYIFEHVND
                     SHEIFFFSTSTVKVAIPLPVILYSPNSGWHFFSSRKLYSNNESFPFFIAKEGENKDKI
                     IEKLSEGSEIVPFDLSITKTVAGMIVVALLLVIVLVKASSRARKNPLAVPRGLQNLVE
                     PVVNFIREDIAIPFIGARHVCYLPFLLTMFFFILVSNLIGLVIPLGINITGNIAVTFV
                     LAFFTFVITSVSGNRQYWSHIFNPPDVPWFMKLPIPLIPFVEFMGIFTKPIILMVRLF
                     ANMFSGHMIVAVLIALIFLMSSVFNLVVGVGTSLISVLFSVFIITVDILVSFIQAYIF
                     TVLSAMYFGTATSEVKH"
BASE COUNT          745 a          491 c          410 g          635 t
ORIGIN      
        1 ccttaatgta agtggctgaa acgaatcagc aaacatttct ttcagatggc caagtttttc
       61 gttggccttc attttttgcc cgacaaggga atcgcgaaaa ccctggtaat ttgttaacga
      121 ataataaagt acacttatat catggtaaca ttccatgagg acatcctctt cagatgcaag
      181 ctcaacaaat gcctttgcat aacgtactgt taccctgtta gtatccatta tctatcaatt
      241 tagttcgata tcgtcaacca attctttaac cagcttttcg tgacgaattt tatcgctcat
      301 ttcggcagaa acaacttttc ctgctatttc gagtgacaat tcagcaacct gatttctaag
      361 atcttccaac gccttggccc gttcaaatgc caattgtttc cgtgtattct caataatttt
      421 atcagcttct tcctgagcct taattttggc atcagaaatg atcgtattgc gttgcaaaac
      481 cccttctttc aacaattgtt ctttttctag tcttgttgcc ttaataatct cctcttgctc
      541 agcttgcagc gatttaattt tttcgttggc ttcggcagcg ccagtaagtg ccctctcgat
      601 attttgctca cgctcggcca atgctttcag caatggtttc cacgcaaatt ttccaagaat
      661 atagaccaac aacaagaaga ttattgttgt ccagattatg gtgcctacat gcggtataag
      721 aaacatgata aaacacttta aataaatacg atatatacca actcaatacg tgcggccata
      781 tactacaata ccgccaacaa aatgcacaga atgatagcca agaaagctac cccttcgata
      841 aatactgaag cgaggatcat actcgaccga atatcagcat ttgcttcggg ctggcgagca
      901 atggcttcca tagctgaagc cccaatttta ccaattccaa ttcctgctgc taatgcggcc
      961 ataccggcac caatacctgc acttaatttt cccagcgcca gttcggctaa aataatgaat
     1021 agtgtcatag catttattgt ttaatgttta acttcagatg ttgcggtccc aaaatacatg
     1081 gctgatagca cagtaaatat ataagcctgt ataaaggaga ccaagatatc gacagtaata
     1141 ataaaaacag aaaaaagcac tgaaattagc gatgttccca ctccaacgac aagattgaaa
     1201 accgacgaca tcagaaagat cagggcaata agaactgcca caatcatgtg acccgaaaac
     1261 atattggcaa ataatcgtac cataagaatt atcggtttag taaatatccc cataaattcg
     1321 acaaacggaa tgagcggaat gggcagtttc ataaaccatg gcacatcggg tgggttaaat
     1381 atatgggacc aatattgcct gtttccgcta acagaggtaa taacaaaggt gaaaaaagcc
     1441 aacacaaaag taacagctat atttcctgtt atgttgattc ccaacgggat aaccaggcca
     1501 atcaggttgc taaccaggat aaaaaagaac atggtcagca aaaacggcaa ataacaaaca
     1561 tgccgggctc cgataaaagg gatagcaata tcttcgcgga taaaattcac tacaggctca
     1621 acaaggttct gaaggccacg cggtaccgcc aagggatttt tgcgggcccg cgacgatgct
     1681 ttaactaaaa caatcacaag taaaagggca accactatca tcccggcaac tgttttggtt
     1741 attgaaaggt cgaaaggcac aatttcggag ccttcactca gtttctcgat gattttatct
     1801 ttgttttctc cttcttttgc aatgaaaaaa ggaaaacttt cattgttgct gtataatttc
     1861 cgggaagaaa aaaaatgcca gcctgaatta ggactataaa gaatcacagg taaaggtatg
     1921 gccactttaa ccgttgatgt ggaaaagaaa aatatttcat gcgaatcgtt aacatgctca
     1981 aagatatact cgaccgtatt gaaatcatta tgttcttcag cctcctcggc ataagaagct
     2041 tttggaacac aaaatgcaat gagcaatatg aaaacacaat atctacacca ctccatacta
     2101 catctcattt tacgtataaa tgtaaggaaa ataacaaaag ctatctccgg cagaagaaat
     2161 aattagcgtt tatgataatt ttttgatgtg ctcttaataa tcagggcttg acgcttaagt
     2221 tttcgattcg ttgatgagtt ccataactga gcgtacttca aaaacagcat aagcaacgta
     2281 c
//
LOCUS       DAOPUW010000302         2278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_50318_length_2278_cov_3.760684, whole genome shotgun sequence.
ACCESSION   DAOPUW010000302 DAOPUW010000000
VERSION     DAOPUW010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50318_length_2278_cov_3.760684"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PKJ43_05875"
     CDS             complement(<1..665)
                     /locus_tag="PKJ43_05875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNZ72130.1"
                     /translation="MGIAACFPGLFTDVTRDAAKYAFVSKEIFETGNWMQISIMGEPY
                     YQKPQLLFWLSAASFQVFGISNFSFKILIFIYSLLGCFAVFKLSERLTDRKTGFISAI
                     MVIFSVIFVLYTMDIHTDTVLFTNVALSLWAIHCWLTNEKPFYAFLSGLALGLSFITK
                     GPFGLLIPIGSAAGFLLWKRKISAKIAQQSILIVFITILIGLLAVVPIFQKNGFNGVW
                     YFL"
     gene            862..2223
                     /gene="argH"
                     /locus_tag="PKJ43_05880"
     CDS             862..2223
                     /gene="argH"
                     /locus_tag="PKJ43_05880"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNZ72131.1"
                     /translation="MSKNNTTTDKIWAKGILIDEIIEKFTIGKDPEMDMFLAPFDVLG
                     SIAHVRMLNTIGLIRDEELPLLIGELISIYNEIGKGNFAIEPGVEDVHSQVELLLTRK
                     LGDIGKKIHSGRSRNDQVLLDLKLFIRSEIKQIVGLSDELFKVLISRSNETKDILMPG
                     YTHLQITMPSSFGLWFGAYAESLTDDMQMMLAAWKTANQNPLGSAAGYGSSFPLNRQM
                     TTDLLGFDNLNYNVVYAQMGRGKVEKNVAFAMSSVAATLAKMAYDVCLFMSQNFNFVS
                     LPDNLTTGSSIMPHKKNPDVFELLRAHCNKLQSLPNEILLIINNLPVGYFRDMQIIKE
                     IFIPAFAEMKTCLNIAAYAVSHLKVNHQILDDKKYDYIFSVEEVNRLVNEGMPFRDAY
                     KHVGQIIMDGNFIPVKKVKHSHEGSLGNLCNDQITEKMNAIVSLFNFSAVDDAIEQLT
                     ANS"
BASE COUNT          723 a          409 c          484 g          662 t
ORIGIN      
        1 cataggaaat accatacacc attgaacccg tttttttgaa aaatgggaac aactgccagc
       61 aagccaataa gtatggttat aaaaacaata agaatagatt gctgggctat tttagccgaa
      121 atttttcttt tccacaaaag aaatccagct gctgacccta tgggaataag taacccaaat
      181 ggccccttgg tgataaagga taagcccaag gcaaggccgc ttagaaaagc gtagaaaggt
      241 ttttcgttcg ttagccagca gtgaatagcc caaagagaga gggctacatt ggtaaaaagt
      301 actgtgtcgg tgtggatgtc catggtgtac agcacaaaaa tcacagagaa tatcaccata
      361 atagccgata taaatcctgt ttttctgtcg gttagtctct ctgaaagttt aaacactgca
      421 aagcagccga ggagagaata gatgaatatc aggattttaa aagaaaaatt ggaaatgcca
      481 aatacttgga aagaagcagc tgaaagccaa aacagaagtt gtggtttctg ataatatggc
      541 tcacccataa tactgatttg catccagttg ccagtttcaa aaatctcttt tgatacaaat
      601 gcatattttg ctgcgtcacg ggtaacatcg gtgaagaggc ccggaaagca tgcggcaatg
      661 cccattataa tcaaggagat aaataatatt aagtcttttt ttgtcaaatt agttagtagt
      721 aaatcataca agagcctgaa cgaaagaaca ggccagccaa agatattaaa cctttttggt
      781 tgtttttggc gcgtggataa agattatttc ctagggtttc atccttttct tatttttgtg
      841 ctgaattatt tgtctacaat catgagcaaa aacaacacaa cgaccgataa aatttgggca
      901 aaaggtatcc tgatcgatga aattattgaa aaattcacca taggaaaaga tcccgagatg
      961 gacatgtttc tcgcaccatt cgatgttttg gggtcgattg ctcatgtgcg tatgctgaat
     1021 accataggtc ttattcgcga cgaagaatta cccttgttga taggcgaatt aatttcgatt
     1081 tataatgaaa ttgggaaagg aaacttcgct attgagcccg gggttgagga tgtacattca
     1141 caagttgaat tgttgttaac ccgaaaactt ggcgatatcg ggaaaaaaat acatagcgga
     1201 cgctctcgca acgatcaggt tttgctcgac ttaaagcttt tcatcaggag cgaaattaaa
     1261 caaattgttg ggctgagtga tgagctgttt aaggtattga tttcgcgaag caacgagaca
     1321 aaagatattc ttatgcctgg ttatactcat ttgcagataa ccatgccgtc gtcgtttgga
     1381 ctttggtttg gagcctatgc agaaagcctt accgatgaca tgcaaatgat gctcgctgct
     1441 tggaaaaccg cgaatcagaa tccattaggt tcggcagcag gatatggttc gagtttcccg
     1501 cttaaccgcc agatgactac cgatttgctt ggttttgaca acttgaatta taatgtggtt
     1561 tatgctcaaa tggggcgtgg aaaggttgaa aaaaatgtgg catttgccat gtcttctgta
     1621 gctgcaacgt tggcgaaaat ggcttacgat gtttgccttt tcatgagcca aaatttcaat
     1681 tttgttagtc ttcccgacaa tttaacgacc ggatcaagca ttatgcctca taaaaagaat
     1741 ccagatgtct ttgaactttt gcgtgcccat tgtaataaac ttcaatcgtt gcccaacgaa
     1801 atattgctca taataaataa tttgccagta ggatattttc gcgacatgca gattatcaaa
     1861 gaaatattca ttcctgcttt tgccgagatg aaaacttgtt tgaacattgc cgcatacgca
     1921 gtttctcacc tgaaagtaaa ccatcaaatc cttgatgata agaaatatga ctacatattc
     1981 agtgttgagg aggtcaatag gttggtaaat gaaggtatgc ctttcagaga cgcatacaaa
     2041 catgtgggtc agataattat ggatggtaat ttcatccctg taaagaaagt gaaacattcg
     2101 cacgaaggaa gcttgggcaa tctttgcaac gaccagatta ctgaaaagat gaacgccatt
     2161 gtttctttat ttaattttag tgctgttgat gacgccatcg aacaacttac ggcaaattca
     2221 taataacctg agttcgattt tatttttaaa gttcagattg gcataaaacg ctgtttta
//
LOCUS       DAOPUW010000303         2277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_50357_length_2277_cov_3.015752, whole genome shotgun sequence.
ACCESSION   DAOPUW010000303 DAOPUW010000000
VERSION     DAOPUW010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50357_length_2277_cov_3.015752"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..1212)
                     /locus_tag="PKJ43_05885"
     CDS             complement(220..1212)
                     /locus_tag="PKJ43_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNZ72132.1"
                     /translation="METYLPSSSRYVRCSYNRCGRSGLKLPMISLGLWHNFGDVDVFE
                     NGRSIVRYAFDQGITHFDLANNYGPSPGSAEENFGQILAKDLKPYRDELVISTKAGYW
                     MWDGPYGEWGSRKYLISSLDQSLKRMGVDYVDIFYHHRPDPETPLEETMMALDHVVRQ
                     GKALYAGISNYNAEDTAKAAKILKELGTPCLINQVKYSMFERWVEDGLLATAEEWGIG
                     VIAFSPLAQGLLTDRYLQGIPEGSRASKSWGFLQPHQVEPALDKVRQLNEVAISRGQS
                     LAQMALAWLLKDKRVTSVLVGASSVAQLANNLGALDNAGFTNDEIERIIAILNK"
     gene            1336..1914
                     /locus_tag="PKJ43_05890"
     CDS             1336..1914
                     /locus_tag="PKJ43_05890"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNZ72133.1"
                     /translation="MSKIHFKPGTMIYPLPAVMVSCGETPEEFNIITVAWTGTICSNP
                     PMCYISVRPERFSHGIIKRTGEFVINLTTEKLAHATDWSGCRSGRKFNKWEECHLTPG
                     QASVVKAPIIEESPVNIECRVKEIISLGSHDMFISDVVNISVDDQYFNETTGKLNLSS
                     AKPLVYNHGFYFGIGKEIGKFGWSVEKKSKKK"
     gene            1914..>2277
                     /locus_tag="PKJ43_05895"
     CDS             1914..>2277
                     /locus_tag="PKJ43_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family peptidase"
                     /protein_id="HNZ72134.1"
                     /translation="MATEKNPLLSFFDTPFETVPFNLIKPEHFLPALNVIIARARKVF
                     KETILNSEEPDFQNTIIPIEKCYDEISKTGSILFNLNSAATSPEIQAITQKVSPKITR
                     FMAQLMVDYTYFKRVEKVY"
BASE COUNT          709 a          517 c          402 g          649 t
ORIGIN      
        1 aaaaaaggct tttatcgact tggcatcaag tgtccctgct gccgtgcatt cctcagccgc
       61 ctcaagcaag accggggtta catcatcgtc gtcatcttta tcgcaagcaa caaaaacgaa
      121 gcttgacaaa aaaacaactg ccaaaaattt tactaatact ttcatagtta accatttaat
      181 tctctaccct aatattttcc ccaataataa ttatacgttc tatttattta gtatggcgat
      241 gattctttca atttcgtcgt tggtaaaccc agcattgtca agagcaccaa gattgtttgc
      301 caactgcgct acagagcttg ccccaacaag caccgaagtt actcttttgt cttttaaaag
      361 ccaagctaaa gccatttggg ctaatgattg tccacgagaa atagctactt cgttcagctg
      421 tcttacctta tctaaggccg gttcgacctg gtgaggctgt aaaaatcccc acgatttaga
      481 cgctcttgaa ccctctggaa tcccttgcaa atacctatca gttaacaagc cttgagcaag
      541 aggtgaaaaa gcaataacgc ctatgcccca ctcttctgca gtagcaagaa gtccatcttc
      601 aacccaacgt tcaaacattg aatatttcac ctgatttatc aaacaaggtg ttcccagttc
      661 tttaagaatt ttagccgctt tggctgtatc ttcagcattg tagtttgaga ttccggcata
      721 tagggctttc ccttgcctca caacatggtc aagagccatc attgtttctt ccaacggagt
      781 ttcagggtcg ggccgatggt gataaaaaat atcaacataa tcgactccca tacgttttag
      841 actttgatct aaactagaaa tcaaatattt acgagacccc cattctccat aaggtccatc
      901 ccacatccaa tatccggctt ttgtcgaaat aaccaactcg tcacggtaag gttttaagtc
      961 cttggccaag atctgcccaa aattctcttc agccgacccc ggtgaaggac cataattgtt
     1021 cgccaaatcg aaatgggtaa taccttgatc gaaagcataa cgtactattg aacggccatt
     1081 ttcaaaaaca tcgacatctc caaagttatg ccacagacct aacgatatca ttgggagctt
     1141 aagccccgat ctgccgcacc tgttgtatga acagcgcaca taacgacttg aagaaggtaa
     1201 ataggtctcc atattgattt attattcttc aaaaatacaa tatttttttt accttgctcg
     1261 ctctgtcacc ttctgtgaaa aacttatccc tgtcaaaaac gtttatattt gcagcaaacc
     1321 actaagtaac gaaatatgtc gaaaattcat ttcaaaccag ggacaatgat ttatccctta
     1381 ccggccgtca tggttagttg tggcgagact ccggaagaat tcaatatcat cactgtggct
     1441 tggactggaa caatatgcag taaccctcca atgtgctata tttcggtgag gccagaacgt
     1501 ttttctcatg gaattatcaa acgtactggt gaatttgtca tcaaccttac aactgagaaa
     1561 cttgcccatg caaccgactg gagcggttgt cgctcgggta gaaaattcaa caagtgggaa
     1621 gaatgccatt taacacctgg ccaagcatcg gtagttaaag caccaattat tgaagagtca
     1681 ccagtcaaca tcgaatgccg ggttaaagaa atcatttcat taggttctca cgacatgttt
     1741 atttctgatg tggtaaatat tagtgtcgat gatcaatatt tcaacgaaac aaccggcaaa
     1801 cttaaccttt cgtcggctaa accattggtc tataatcatg gtttctattt cggaattggt
     1861 aaagaaattg gcaaatttgg atggtcggtc gaaaaaaaat caaagaaaaa ataatggcaa
     1921 cagaaaagaa ccccttgtta tcgtttttcg atactccttt tgaaactgta cctttcaacc
     1981 taattaagcc tgagcatttt ttgccagcac tcaacgtaat aattgcccgg gcccgaaaag
     2041 ttttcaaaga aacaattttg aacagtgaag agcctgattt tcagaataca attatcccaa
     2101 tagagaagtg ctacgatgaa atctcaaaaa caggatctat acttttcaac cttaattctg
     2161 ctgcaacatc ccctgaaatt caagcgataa cacagaaagt atcgcccaaa ataactcgtt
     2221 ttatggcgca actaatggtc gattacactt actttaaacg agttgaaaaa gtatatg
//
LOCUS       DAOPUW010000304         2267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_50723_length_2267_cov_2.709765, whole genome shotgun sequence.
ACCESSION   DAOPUW010000304 DAOPUW010000000
VERSION     DAOPUW010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50723_length_2267_cov_2.709765"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PKJ43_05900"
     CDS             <1..315
                     /locus_tag="PKJ43_05900"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HNZ72135.1"
                     /translation="IKGKQTGITRDEFEYSIPVADARQMLGLCLYNPVEKTRYITEID
                     HKTWEIDVFHGQNKGLYIAEIELHSENEPINLPDWIVAEVSTDERYFNFNLSINPYCS
                     WQ"
     gene            369..>2267
                     /locus_tag="PKJ43_05905"
     CDS             369..>2267
                     /locus_tag="PKJ43_05905"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HNZ72136.1"
                     /translation="MMKRMGKISILFAFIMLAILASGSNTTLNDNHENIEKNLQRIKS
                     LLKSREWSPADTAFERQLIALVNYFEHPQIDSVVSTLDSIIEVKEILFVRDRQRIKNP
                     ENVKGYVQHHEISEKLVKIADEVEGLYPPESIIVPEDRFAGGYAMLNIIGENETERML
                     HDSIFIMPDSILSQLHDPLTEQDEARRCYLDSLSNLLINEARLAHNKAVIQAYRDSVT
                     ENYRDNFIQNIIKKEQTIFLDSVYAINNQILAKYNDNETLELNQELKGIINDLLWQIK
                     IQPNQLTILNLNNEPTTLNLQNFAVWHQWVWLKNTRNDSIGIRVEDIDKHSLRILVDE
                     SINLSRLTVKSGVEIEQVKALEENDIKLSKIITRNPELSPWKLKGKAYAGFTQNYINE
                     FWSQGGKSSASALATFNYSANYEKKKLKWENFVDMKVGVIYYLPEEGSTVKRNWHKNS
                     DNIELNSRLGYSAAKKWYCSTEANFKTQLFYGYSNQKDSIVSSALFSPAYLTFSAGME
                     YKPKKNFSMFLSPVSLRTTFVSNPIVNEKGFGLNEGETRKSSIGFSAKIDYSQNLMEN
                     ISFKSKNSMFINYGNNASGELQLFKLPDFDSENSIEFKVNQFITTQVNFHFIYDKDVE
                     SRWTTASGE"
BASE COUNT          775 a          465 c          440 g          587 t
ORIGIN      
        1 attaaaggta aacaaacagg cattacccgc gacgaattcg agtactcaat cccggtagca
       61 gacgcccgcc aaatgctcgg tttgtgcctc tacaatccgg tagaaaaaac acggtacatt
      121 actgaaatcg atcataaaac atgggaaatc gatgtttttc atggccaaaa caaaggtctg
      181 tatattgcag aaattgagct acattcagaa aatgagccta tcaatcttcc agattggatt
      241 gttgctgaag taagtaccga cgaacggtat ttcaatttta acttgtcgat aaatccatac
      301 tgtagctggc aataaaaaac gttcaattca gttttgaaag agcatgatca tattttttgg
      361 gggaaaacat gatgaaacgg atggggaaaa taagcatact ttttgctttt ataatgctgg
      421 caatactggc atctggaagc aacaccacac tgaacgataa tcacgaaaat atagaaaaga
      481 accttcagcg cataaaaagt cttttaaaat cgcgtgaatg gtctccggct gacactgcct
      541 ttgaacgaca actcattgca ctggtcaact actttgaaca ccctcagata gattcggttg
      601 tgtcaactct cgattccatt atcgaggtaa aagaaatttt gtttgttcgt gaccggcaga
      661 gaattaaaaa cccggaaaat gtaaaaggat atgttcagca tcacgaaata tcggaaaagc
      721 ttgtaaaaat tgccgatgaa gtagaaggac tttacccacc agaaagcata attgtgcccg
      781 aagaccgttt tgccggtggc tatgccatgc tcaacatcat cggtgagaac gaaaccgagc
      841 gaatgttgca cgattcaatt tttatcatgc ccgactcaat cctaagccaa ttacacgacc
      901 cgctaaccga gcaggacgaa gcccggcgct gttacctcga ctctctctcc aatctattaa
      961 taaatgaagc ccgccttgct cataataaag cggtgattca agcctatcgc gattctgtaa
     1021 ccgaaaacta ccgcgataac ttcattcaga atattattaa aaaagaacaa acaatatttc
     1081 tcgattcggt gtatgccatt aacaatcaaa tattagcaaa gtataacgac aacgaaaccc
     1141 ttgaattgaa ccaggaattg aaaggaataa tcaatgacct gctgtggcaa attaaaatac
     1201 aacccaacca actaacaatc ttaaacctaa ataacgaacc tacaaccctg aaccttcaga
     1261 attttgccgt ttggcaccaa tgggtctggt tgaaaaacac ccgtaacgat tctattggca
     1321 ttcgtgttga ggatatcgac aagcatagtt tgcgcattct tgtcgatgag tcgatcaacc
     1381 tttcccggct tacagtgaaa agcggcgttg aaatagaaca agttaaggca ttggaagaga
     1441 atgatatcaa actaagtaaa ataattaccc gtaaccctga actcagtcca tggaagctta
     1501 aagggaaagc ttatgcggga tttactcaaa actatatcaa cgagttttgg tcacaggggg
     1561 ggaaatcttc ggcatctgca ttagcaactt tcaattattc agcaaattac gaaaagaaga
     1621 aactaaaatg ggaaaatttt gttgacatga aagttggggt aatttattac cttccagaag
     1681 aaggttctac cgttaaacgc aactggcaca aaaactctga caatattgaa ttaaactccc
     1741 gattgggcta ctcggctgca aagaagtggt attgtagtac cgaagcaaac ttcaaaaccc
     1801 agttgttcta tggatattca aatcagaaag atagtattgt aagttctgcg cttttctcac
     1861 cagcttacct tactttttca gccggtatgg agtataagcc caagaaaaac ttctcaatgt
     1921 tcctctctcc ggtttcactg cggacgacat ttgttagcaa cccaatagtt aatgaaaaag
     1981 ggtttggcct taatgaaggg gaaacaagaa aaagtagcat tgggttttct gcaaaaatcg
     2041 attattctca gaacctgatg gaaaacataa gcttcaaatc gaaaaactca atgtttatta
     2101 actacggaaa caatgcatca ggagaactgc aattgttcaa actccccgat tttgacagtg
     2161 aaaactcaat cgagttcaag gtcaaccagt ttattaccac tcaggtaaac tttcatttca
     2221 tatacgacaa agacgtagaa tcgaggtgga caacagcttc gggtgaa
//
LOCUS       DAOPUW010000305         2266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_50747_length_2266_cov_3.045228, whole genome shotgun sequence.
ACCESSION   DAOPUW010000305 DAOPUW010000000
VERSION     DAOPUW010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50747_length_2266_cov_3.045228"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PKJ43_05910"
     CDS             complement(<1..531)
                     /locus_tag="PKJ43_05910"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNZ72137.1"
                     /translation="MKNWILPLIVLVFSTQLIQAQNLLYTKTGHAYFMSHTEAIDIDG
                     NNNQVTSFLNIETGEIAFQMLVKSFKFTLATAEEHFNETYMESDKYPKAQFKGFVENM
                     QSIDLKKDGTYKAQVKGDLTIHGVSKAIKQEGNIVVKNGKITANSAFTVNIDDYAIKV
                     PKMVEDRVAKNVDIKVD"
     gene            complement(542..1411)
                     /locus_tag="PKJ43_05915"
     CDS             complement(542..1411)
                     /locus_tag="PKJ43_05915"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HNZ72138.1"
                     /translation="MKKIIISGLIVASIGIIKPCSAQDISALLDEETSKTTDYTLATF
                     LGNRVVNGQSSEQLQRNGLDFRIAHRFGSFKEGKNELYGFDESNSYFTVDYGLSNRIM
                     IGLGRATDNKLVTANIKAKLLRQSSGEKNMPVTLSFWGGISATTQKFSNKERNDDFNS
                     RLEYTGQLLIARKFNNLSILVSPTYLHRNLVDKQDDANDLTALGLSGRYKISSVFDVA
                     LEYFWVNQPKNSSTAYYNPLSFAMSYQVSHHVFQVVVSNSHAIAENSVIGRTVNSWTK
                     GDIRLGFNISTVF"
     gene            complement(1419..2183)
                     /locus_tag="PKJ43_05920"
     CDS             complement(1419..2183)
                     /locus_tag="PKJ43_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72139.1"
                     /translation="MIKNHICAVALILSVLTGCEYENAEEKYYDAKGPVVVNNNGLLA
                     HIPFDKNISDKSENKSAVALQGDAVYVKGVDGKDSSAIKLNGYPQCIAISNIGYNDTL
                     SIFLWFKTEQALTKNDKFTLFDYGVNSFSMQIDGSTGSTLLNTIHNNEKATMEDWINS
                     FNGWNYLYAESGSGKFKVVYKGVMKNNEVIEIDTENESIGALLPVADMLYIGRSGGQN
                     TASETYFSGSIDNIRVYNRPLSKSELLALINNDTQH"
BASE COUNT          581 a          446 c          446 g          793 t
ORIGIN      
        1 atctactttt atatcaacat tttttgctac acggtcctca accattttag gaacttttat
       61 tgcgtaatca tcaatgttta ccgtgaacgc agagttcgcc gtaattttgc cgttctttac
      121 tacaatattg ccttcttgct ttattgcttt gcttacccca tgtatagtta aatcgccttt
      181 tacctgagcc ttgtatgtgc cgtctttttt caggtcgatc gattgcatgt tttcaacaaa
      241 ccccttgaat tgtgctttgg gatatttgtc tgattccatg taggtttcgt tgaaatgttc
      301 ttcggctgtg gctaaggtga atttgaatga tttaaccagc atctgaaatg ctatctcgcc
      361 ggtttctata ttcaagaagc tggtaacctg attgttgttc ccatcaatat cgatagcttc
      421 ggtgtgcgac atgaaatagg catgaccggt ttttgtgtaa agcaggtttt gagcttgaat
      481 tagttgtgtg ctgaaaacca atactattaa tggtagaatc caatttttca tacgtttaat
      541 tttagaatac ggttgaaata ttaaagccca aacgaatgtc acctttggtc caactgttta
      601 ctgttcggcc tattacacta ttttcagcaa tggcgtgtga attgctgacc acaacttgaa
      661 aaacatggtg gctaacctga tacgacattg caaaagaaag tggattgtaa taggctgtac
      721 tgctgttttt aggttggttt acccaaaaat attcaagggc aacatcgaag accgatgata
      781 ttttataccg accgctaagt ccgagtgctg tcaaatcgtt agcatcatcc tgtttatcaa
      841 ctaaattgcg gtggagatat gttggcgaaa ccaatattga taaattattg aactttcggg
      901 caatgagcaa ctgtccggta tattccagcc ggctgttaaa atcatcgttt cgctctttat
      961 tcgaaaattt ttgcgttgtt gccgatattc caccccaaaa tgaaagtgtc accggcatat
     1021 ttttttcacc gctcgattga cgtaacaact ttgctttaat gttcgcagtg accagtttat
     1081 tatcggtagc acgtcccaat ccaatcatta ttcggttcga tagcccgtaa tcaactgtga
     1141 aataagagtt tgattcgtcg aatccataaa gttcgttttt cccttcttta aacgaaccaa
     1201 agcggtgtgc tatcctgaaa tcaagtccat tgcgctgaag ctgttcgctc gattgtccgt
     1261 taacaacccg gtttccaaga aatgtagcca gggtataatc ggtcgttttg cttgtttctt
     1321 cgtcgagtaa agcgctgata tcttgcgctg aacatggttt aattatgcct atcgatgcta
     1381 cgataagtcc tgatataatt atttttttca tttgctcttt agtgctgagt gtcgttattg
     1441 attaaggcta aaagttccga tttggaaaga gggcggttat ataccctgat gttgtcgata
     1501 cttcccgaga aataggtttc ggatgcagtg ttttgccctc ccgaacgacc aatatacaac
     1561 atgtctgcaa caggaagtaa tgctccaatc gattcatttt cagtatctat ttcaattact
     1621 tcgttgtttt tcatcacccc tttgtaaaca accttaaatt ttcctgatcc actttcggca
     1681 tataagtaat tccatccatt gaacgagttg atccagtctt ccatggttgc tttttcatta
     1741 ttatggatgg tgtttaagag cgttgagccg gttgagccgt caatttgcat cgaaaaactg
     1801 tttacaccgt agtcgaatag ggtgaattta tcgtttttgg ttaaggcttg ttccgttttg
     1861 aaccataaga atatcgataa agtatcgttg tagccgatat ttgaaatggc gatacattgc
     1921 ggatagccat tgagcttgat ggcactgctg tcttttccgt cgacaccttt aacatacacc
     1981 gcgtctccct gaagggctac agcgctcttg ttttcacttt tatcgcttat gtttttatca
     2041 aagggaatat gggcgagaag tccgttgtta tttactacaa caggcccttt ggcatcgtaa
     2101 tatttctcct cggcattttc atactcgcaa cccgttaata cggaaagaat taaagccacg
     2161 gcacaaatgt ggttttttat catggtttgg ttttgttaat ttcggcttcg aatatgagta
     2221 ttttttgtta gatagaaatt caaatttgaa tcattttgta tcattt
//
LOCUS       DAOPUW010000306         2266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_50755_length_2266_cov_2.753053, whole genome shotgun sequence.
ACCESSION   DAOPUW010000306 DAOPUW010000000
VERSION     DAOPUW010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50755_length_2266_cov_2.753053"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /gene="coaE"
                     /locus_tag="PKJ43_05925"
     CDS             <1..523
                     /gene="coaE"
                     /locus_tag="PKJ43_05925"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNZ72140.1"
                     /translation="FDQLGIPVFYADEEAKRFLNEDDAIREYIIELFGEDLYNTNNKL
                     IRPKLASLIFNDKLALSKINSFLHPVVINYFNQWADQQKGPYVIEEAALIYESGHDKL
                     FDKIIVVTAPLEIRISRVMERDCTSREKVLERINNQLDEETKKEKADFIIENDNRQML
                     IPQIINIHNLLLS"
     gene            523..1263
                     /locus_tag="PKJ43_05930"
     CDS             523..1263
                     /locus_tag="PKJ43_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HNZ72141.1"
                     /translation="MAKFGKWIGAGLGAFAGGPIGAILGFVIGSAFDAGSEVNTYQTG
                     QYSARTNNPTTGGYAMSLLVLVAAIMKADGQAMRSELNYVREYFTRNFGVRSANEAIQ
                     LLRDLLQQNIPVTDVCHQIQANMDYSSRLQLVHFLFGIANSDNILSDKELKLIAHIAV
                     NLGISSKDYESIKAMFVKNTDFAYKILEIDPNVSDEEVKKAYREMAKKYHPDKVSYLG
                     EDFQQAAKDKFQKVNEAYEEIKKQRNML"
     gene            complement(1297..1680)
                     /locus_tag="PKJ43_05935"
     CDS             complement(1297..1680)
                     /locus_tag="PKJ43_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HNZ72142.1"
                     /translation="MSLENFENSDFDGSKFREEVYSKSVRAGKRTYFFDVKMTRKGEY
                     YLTITESKRKNNLSGSAFYEKHKIFLYREDFEKFTDGLSFIIDFINEKQPLDSLSHFS
                     GDDAEDGQSDELPEYSDIDFDDLTK"
     gene            complement(1775..>2266)
                     /locus_tag="PKJ43_05940"
     CDS             complement(1775..>2266)
                     /locus_tag="PKJ43_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNZ72143.1"
                     /translation="MVNAWAMAGGKVVFYSGIMPVCRDENGVAVVMGHEIAHVVARHS
                     NERMSHGLITQLGGMALDVALKQKPEATKNIFLSAYGIGSQVAVTLPFSRKHEYEADE
                     LGLIFMTMAGYTPQKAIEFWTRMSEMGKSNVPEFLSTHPLDSKRIAALAKLMPKMETY
                     KPK"
BASE COUNT          751 a          440 c          419 g          656 t
ORIGIN      
        1 atttgaccaa ttgggaattc cagttttcta tgccgacgaa gaagccaaac gctttcttaa
       61 tgaggacgat gcaattagag agtatattat tgaattgttt ggagaagatc tttacaacac
      121 aaacaacaaa ttaatccgac caaaattagc atctctaatt tttaacgata aattggcact
      181 ttcgaagatc aattcatttt tgcaccctgt tgttataaac tattttaacc aatgggccga
      241 tcagcaaaaa ggaccttatg taattgaaga ggctgctttg atttacgaat cggggcatga
      301 taagctgttt gataaaatca ttgtagtaac agctcccctc gaaatccgca tcagtagggt
      361 aatggagcga gactgtacct cgcgggaaaa agtgctggaa agaattaata accagctcga
      421 tgaagaaaca aaaaaggaaa aagcagactt tataattgaa aatgacaatc gacaaatgct
      481 tattcctcaa ataattaata tacacaactt attgctttca taatggcaaa atttggaaaa
      541 tggataggtg ccggattagg ggcatttgca ggtggtccca taggggcgat tcttggtttt
      601 gtaattggat cagctttcga tgccggttct gaggtaaaca cctatcaaac aggacaatat
      661 tcagcaagaa caaataatcc gacaaccgga gggtacgcaa tgagtttgct ggtattggta
      721 gctgcaatca tgaaggccga tggacaagcc atgcgttcag agttaaacta tgtaagggaa
      781 tactttacac gaaattttgg cgtacgatca gccaatgaag ccatccagct tttacgcgac
      841 ttgctgcaac agaatattcc tgtaactgat gtttgtcacc aaatacaggc aaacatggat
      901 tattcgtcga gacttcaact tgttcacttc ctgtttggca ttgctaattc agacaatata
      961 ctatcagaca aagaattaaa actaattgca cacattgcag ttaacttagg aatctcttca
     1021 aaagattatg aatcgatcaa agcaatgttt gtgaaaaaca ccgactttgc ttataaaatt
     1081 ctggaaatcg atcctaatgt aagcgacgaa gaggtgaaaa aagcctatcg tgaaatggca
     1141 aaaaaatacc atcctgataa agtaagttat ttaggtgaag atttccagca agcagctaaa
     1201 gacaaattcc aaaaagtaaa cgaagcttac gaagagataa aaaagcaaag gaatatgcta
     1261 taaagattta ctaacaaaaa aaccgcgttt tgcggtttat tttgttaagt catcaaaatc
     1321 aatgtctgaa tattcaggca attcatcgct ttgaccatct tcagcatcat cgccagagaa
     1381 gtggctcaaa ctgtccaatg gttgtttttc gttgatgaaa tcgataataa aactcagtcc
     1441 atcagtaaat ttttcaaaat cttcgcgata aagaaaaatc ttgtgttttt cataaaaagc
     1501 acttccactg aggttattct tccgtttact ttctgtaatc gtaagataat actcaccctt
     1561 acgggtcatt ttaacatcga aaaagtaagt gcgttttcca gccctcacag actttgaata
     1621 aacttcttct ctaaatttac tgccatcaaa gtcgctgttc tcgaaatttt ctaagctcat
     1681 attctaaagt cagtttagga taatcgttca aatatataaa aaaaattatt ctaaactagt
     1741 aatgaacata agctaaaaaa cagttttctt atttttattt aggtttatac gtctccatct
     1801 ttggcattaa tttagccaaa gcagcaattc tttttgaatc aagagggtgt gtactcaaaa
     1861 attcgggtac gtttgatttg cccatttccg acatacgagt ccagaattca atagcctttt
     1921 gaggggtata cccagccatt gtcataaaaa taagtcccag ttcatcagct tcgtattcgt
     1981 gtttgcgaga gaaaggcaat gtaacggcaa cttgtgaacc tattccgtat gccgaaagga
     2041 aaatattctt tgtcgcttcg ggtttttgtt tcaatgccac gtcgagagcc atgcctccta
     2101 actgggtgat caatccatgg ctcattcgtt cgttactgtg ccgggctacc acgtgagcaa
     2161 tctcgtgccc cattacaacg gcaaccccat tttcgtcacg acaaacaggc ataattcccg
     2221 aatagaaaac aactttacca ccagccattg cccaggcatt caccat
//
LOCUS       DAOPUW010000307         2249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_51350_length_2249_cov_3.017320, whole genome shotgun sequence.
ACCESSION   DAOPUW010000307 DAOPUW010000000
VERSION     DAOPUW010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51350_length_2249_cov_3.017320"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PKJ43_05945"
     CDS             complement(<1..206)
                     /locus_tag="PKJ43_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72144.1"
                     /translation="MEDLAMTLVKRNQNYYPTFPSFFDNFLNRGLMDWDNWNFSDTNT
                     TIPAVNVKEDNDKFLIEVAAPGMK"
     gene            776..1672
                     /locus_tag="PKJ43_05950"
     CDS             776..1672
                     /locus_tag="PKJ43_05950"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF006307.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HNZ72145.1"
                     /translation="MIVTIIGLGLIGGSIAVDLRKTGFATELIGFDNSEKHCEKALQL
                     GLVDQIKPLQQAVKGVDIVVVAVPVDKIAVILPEVLDLIDPSTSVTDVGSTKHVICQG
                     VDNHPRRGNYVPAHPMSGTENSGPEAAMEGLFKEKICIICDQEQAKPQHLALIEKMYH
                     TIGLDLAYMTSDEQDHSTAHVSHLPHATSFALANAVLDVNDRHIIFDLASGGFQSTVR
                     LAKSSPEMWGPIFMQNKNYVVESLELYIKHLEEFKQSLESENDEKLMELMRNANRIRE
                     VLDGESQELVRLQSTTTKLYRK"
     gene            1693..>2249
                     /locus_tag="PKJ43_05955"
     CDS             1693..>2249
                     /locus_tag="PKJ43_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNZ72146.1"
                     /translation="MSIELDILPVKSWLPSIDNPLLIAGPCSLETEEQTLATARELAK
                     DKRVFVFRGGVWKPRTRPGSFEGIGSIGLEWLKRVKEETGLPVATEVANAQHTEECLK
                     AGVDVVWIGARSTGSPFAVQEIADVLKGTDTVVAVKNPMNPDLSLWMGALERLNRVGI
                     NKLVAIHRGFSPFQETKYRNYPGWKT"
BASE COUNT          682 a          396 c          486 g          685 t
ORIGIN      
        1 tttttcatac ccggggcagc aacttcaatt aggaacttat cgttgtcttc tttcacattc
       61 actgccggaa tagtagtgtt tgtgtctgaa aagttccaat tatcccaatc cattaatcct
      121 ctgttcaaaa aattatcgaa gaacgacgga aaagtggggt agtaattctg atttcttttt
      181 acaagtgtca tggctaaatc ctccatttat ttaagttaaa cgtttattaa tttatacgcc
      241 agtcaatttt tcaaagagaa tgccaagcgc gaaaagtgga aaaaatgaca tgaagttttc
      301 tgaaaaggag taaaaagtat gaaattttgt cgttgtcaat cagttttcta cgtcattttt
      361 tccgatttaa gccctataaa agattctttt ctgaaaattt aaagggaata gcacctttta
      421 tataataaat tgaaagttta aagttgattt tagtttgaaa tcttaaatta tttgcaattt
      481 ttctaaacta tttcgttctg atgttgttct gtcgttaaat aataaattat atttgcgcca
      541 gtaaaagcat atttgctacc tgaaattgaa attattgtca atgaaaagag tgctgtaggc
      601 tttactaaaa attgaaataa atagttttgt gatgaagaat tataacaaca cattttttta
      661 cttttattat tatttcggta ttcatatcgg aatcgagtag atgtgtgttt gttaaacaag
      721 agatacaatt tataaggttc cgaatttcgg agcctttttt tttaattata cagatatgat
      781 tgtaacaata ataggattag ggcttattgg ggggtcgatt gcagtagatc tcagaaaaac
      841 agggtttgcc actgaattaa ttggtttcga taattcggaa aaacattgtg aaaaggctct
      901 tcaacttgga cttgttgacc agataaagcc cttgcaacag gcggttaaag gggttgatat
      961 tgtggtggtt gctgttcctg tggataaaat tgccgtgata ttgccagaag ttcttgatct
     1021 catcgatcct tcaacctcgg taaccgatgt cggttcaact aaacacgtaa tatgtcaagg
     1081 tgttgataac catccgcgaa gaggaaatta cgtgcctgct cacccgatgt cggggacaga
     1141 aaattctggt cctgaagctg caatggaggg gcttttcaaa gaaaagattt gtattatttg
     1201 tgatcaggag caagctaaac cacaacattt ggcacttatt gaaaagatgt atcataccat
     1261 cggcttagat ttggcatata tgacctccga tgagcaagat catagtactg cccacgtttc
     1321 gcaccttccg catgctacct cttttgccct agccaatgca gtgctcgatg tgaacgatcg
     1381 gcacattatt ttcgacctgg caagcggggg cttccagtca accgttcgtt tggcaaaaag
     1441 ctcacctgaa atgtgggggc caattttcat gcaaaacaaa aattatgtgg ttgagtcgct
     1501 cgaactgtat attaagcatc tcgaagaatt taagcagagt ctcgaatcag aaaacgatga
     1561 gaaacttatg gaactcatgc gaaatgctaa tcgaattcgt gaagttctcg acggcgagtc
     1621 acaggagttg gtaaggttgc agtcgacaac aacaaaactg tacagaaaat agaaatataa
     1681 aaaacaaaca atatgagtat tgaattagac attttaccag ttaaaagctg gcttccatca
     1741 atcgataatc cattattgat tgctggtcct tgtagtttgg aaactgaaga gcaaaccctg
     1801 gcaacagccc gagaattagc taaagataag cgtgtattcg tttttcgtgg tggtgtatgg
     1861 aaaccccgta ctcgaccggg atcatttgaa ggcattggct ctattgggct cgaatggctg
     1921 aaaagggtga aagaagaaac tgggcttccg gtggctaccg aggtggccaa cgcacagcac
     1981 accgaggaat gcctgaaagc tggggttgat gtggtttgga ttggtgcccg ttcaacaggc
     2041 agcccttttg ctgtgcaaga aattgccgat gtattgaaag gcactgatac cgttgtggct
     2101 gtaaaaaacc cgatgaaccc cgatctctcg ttatggatgg gggctctcga acggcttaat
     2161 cgtgtaggta ttaataaatt ggttgctatc catcgcgggt tttccccttt tcaggaaact
     2221 aaatatcgca actatccagg ttggaaaac
//
LOCUS       DAOPUW010000308         2247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_51420_length_2247_cov_3.221715, whole genome shotgun sequence.
ACCESSION   DAOPUW010000308 DAOPUW010000000
VERSION     DAOPUW010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51420_length_2247_cov_3.221715"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1288)
                     /gene="fucP"
                     /locus_tag="PKJ43_05960"
     CDS             complement(65..1288)
                     /gene="fucP"
                     /locus_tag="PKJ43_05960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HNZ72147.1"
                     /translation="MSKHKLTTKENLLPFILITALFFLWGMAHGMLDVLNKHFQTILD
                     ISKAKSGMIQFAMYIAYFLMALPAGYFMRRFGYKKGIILGLSLFAVGSLLIAATTGFE
                     SFWLFLIFLFVLGCGLATLETAANPYTTKLGPSESSERRINFSQSFNGLAWTVGPLIG
                     LFIYGNSSDVAGQKLSSLTLPYMIIGFVVFAVAVVFMLSKVPEIQEDADDPISETVAQ
                     KPLIKDRHLVLGVIAQFAYCAAQTGVFSYLINYVTDESQPVHFDVANGPYFLSIGFAL
                     FMVGRMSGSFLMSYFKPARMLGLYALMCCLLLPVVTANLGWASIIALYGIFFFMSIMF
                     PTIFALAIKDLGPKTKKASSFIIMSIVGGAVFPPLMGYIADVTHSISVGFLAPIPFFA
                     YILYYAIEGHKVKTT"
     gene            complement(1333..1767)
                     /gene="fucU"
                     /locus_tag="PKJ43_05965"
     CDS             complement(1333..1767)
                     /gene="fucU"
                     /locus_tag="PKJ43_05965"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HNZ72148.1"
                     /translation="MLKGISPLLSPELLATLTRMGHGDEIVLADAHFPGESFNDNVLR
                     ADGLRIPDLLEAILPLFELDSYVPHPLVMMAAVEGDLLDPSVEESYLKAIHKSNPDAP
                     AIERIDRFAFYERTKSAFAVLMTGETAKYGNILLKKGVTPIT"
     gene            complement(1788..>2247)
                     /locus_tag="PKJ43_05970"
     CDS             complement(1788..>2247)
                     /locus_tag="PKJ43_05970"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNZ72149.1"
                     /translation="NGFSKIAYLSGIPSVHLFSERQEGYRQGVKAEGIQYENCVVMSN
                     GFTVQQGYEATMQLFGEKDKPDALIFDSHLLAWGAIQYMRTGKKKLLDKMAFASFGGY
                     PWLSYAVPNSVMIQQPEEKMAEASFRLLMEELANPGANEGQILKFDADLY"
BASE COUNT          722 a          476 c          441 g          608 t
ORIGIN      
        1 gattggagct tcaatgattt cgattttatt gattcctttt aaaactgatt ttcgcatcct
       61 attcttacgt tgtttttact ttatgccctt caattgcata atataaaata taggcaaaaa
      121 atggtattgg agccaagaat ccaaccgaaa ttgaatgggt cacatcggca atatagccca
      181 ttagcggagg aaaaacagcc ccgcctacga tggacataat aataaacgac gatgcctttt
      241 ttgtttttgg accaaggtct tttattgcca gcgcaaaaat ggtcgggaac attatggaca
      301 taaaaaagaa gataccatat agtgctataa tagaagccca acccaaatta gcggtaacta
      361 ctggaagcaa cagacaacac atcagtgcat ataagcccaa cattcgcgcg ggtttgaaat
      421 aggacatcaa aaagctgccc gacatacggc caaccataaa caatgcaaaa ccaatggata
      481 aaaaataagg tccgttggca acatcaaaat gaacaggttg actttcgtcg gtaacataat
      541 tgatcaggta gctaaatacc ccagtctgag cagcacaata ggcaaattgg gcaataactc
      601 ccaacactag atgcctatcc tttatcaatg gtttttgagc aacagtttca gaaattggat
      661 cgtcggcatc ttcttgaatc tctggcactt ttgatagcat aaatactact gccactgcga
      721 atactacaaa gccgatgatc atataaggaa gagtaagcga gcttagtttt tgaccagcta
      781 catcggaact atttccatat ataaaaaggc caattaaggg acccacagtc catgctaatc
      841 cattgaacga ctgcgaaaaa ttaattcgac gttcactgct ttcagaagga ccaagctttg
      901 ttgtgtaagg gtttgccgct gtttcgagag tagcaagtcc gcagccaagc acaaagagaa
      961 agatgaggaa taaccaaaat gattcaaaac cagttgttgc tgcaattaat aacgatccaa
     1021 cagcaaaaag cgaaagtcct aaaataatcc cctttttgta tccaaaacgc cgcataaaat
     1081 aacccgcagg aagagccatt aagaagtaag ctatatacat agcaaattgg atcattcctg
     1141 attttgcttt tgaaatatca agaattgttt gaaaatgctt gtttaaaaca tcaagcatac
     1201 catgtgccat tccccataga aaaaacaaag ccgtgatgag gatgaagggt aacaggtttt
     1261 cttttgtcgt tagtttatgt ttagacatat cttcaagttt tcgacttatt agattaaata
     1321 gcagttaaaa acctaggtaa taggtgttac cccttttttc aggagaatat tcccgtattt
     1381 agctgtttcg ccagtcataa gtacagcaaa ggcactttta gtacgttcgt aaaatgcaaa
     1441 acggtcaatg cgttcaatcg ccggagcatc ggggtttgat ttatgaatcg ccttaagata
     1501 gctttcttcg acagaagggt cgagcaggtc gccttcgaca gcagccatca taaccaaggg
     1561 atgaggcaca taactgtcga gttcgaaaag cggaagtata gcttccagta aatcagggat
     1621 gcgaaggcca tcggcacgca aaacattgtc attgaacgac tcgccgggaa aatgagcatc
     1681 ggccagcact atttcatcgc catgtcccat acgggtcagc gtggccagta attcgggact
     1741 caataatgga gaaattcctt ttaacatagt tattgtgttt tataatatta atataaatct
     1801 gcatcaaatt tcaagatttg cccttcattt gcgcccgggt ttgcgagctc ttccatcagc
     1861 aggcgaaatg aagcttcagc cattttttct tcgggctgtt gaatcattac cgaattcggc
     1921 acggcataag aaagccaggg atagccgcca aaacttgcaa aagccatttt gtcgagcagt
     1981 ttctttttcc cggttctcat atattgaatg gccccccaag caagtaaatg tgagtcaaat
     2041 atcagcgcat cgggtttatc tttttcaccg aaaagttgca ttgtagcctc gtaaccctgc
     2101 tgaacagtaa atccattgga cataaccaca caattttcgt actgaattcc ttcagctttt
     2161 accccttgcc tatacccttc ctgacgctca gaaaacaagt gcacagatgg gatacccgat
     2221 aaataagcaa tttttgaaaa cccgttt
//
LOCUS       DAOPUW010000309         2220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_52332_length_2220_cov_4.123326, whole genome shotgun sequence.
ACCESSION   DAOPUW010000309 DAOPUW010000000
VERSION     DAOPUW010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52332_length_2220_cov_4.123326"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PKJ43_05975"
     CDS             complement(<1..598)
                     /locus_tag="PKJ43_05975"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HNZ72150.1"
                     /translation="MLTENDIKQIETQGLTLEELEKQIQHFQKGFAPIKLIGPATPEN
                     GIKILSEEDITRMISVFENSLSSGLLALKFVPASGAASRMFKNLYSFLEKAANAENVE
                     QLAESDSFIKTFFANIEKFAFYNSLSKITVNQDDKTEWVKNLLMPEGLGYGQKPKGQL
                     AFHKYGDSFRTPFEEHLVEGASYCTGTNGMAQVHFTVSP"
     gene            745..1701
                     /locus_tag="PKJ43_05980"
     CDS             745..1701
                     /locus_tag="PKJ43_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420872.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HNZ72151.1"
                     /translation="MTKVTKIYAWGHQKRYNDFSNHFKILFKERIQKVSVDAGFTCPN
                     RDGLKGIGGCTFCNNKTFNPSYCQLSKSISAQLGEGISFFRRKYDSMKFLAYFQAYTN
                     TYAPVADLERMYSEALSVPDVMGLVVATRPDCLSNDVLDLLQAFAEKYYVMVELGVES
                     CENDTLARINRGHTFEESVAAIEALAARSIHNCAHLILGLPGENRSVILNQARKISAL
                     PVENIKLHQLQIHRDTVMAHQYVTHPGQFDIYDSAADFVELIVDYVELLRPSIVIERF
                     VSESPAKLLIAPDWGIKNFEFVAKVEKRLAERDTWQGRLYQP"
     gene            complement(1752..>2220)
                     /locus_tag="PKJ43_05985"
     CDS             complement(1752..>2220)
                     /locus_tag="PKJ43_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNZ72152.1"
                     /translation="TMPMVYENRYLNTDNSKKWDFSQIEPEIVSICLGTNDFSDGDGI
                     HERHPFDAEIFTSTYIKFVETVYNHYPDTQIALLSSPMLSGDKSELLLKCLNNVKNHF
                     SRTKYKPIAIFSFDGIVPHGCDYHPDIDDHMQMADQLIPFYQDLLGINGDITD"
BASE COUNT          596 a          411 c          479 g          734 t
ORIGIN      
        1 cgggcgaaac cgtaaaatgc acttgtgcca tgccatttgt gccggtgcaa tacgaggctc
       61 cttctaccaa atgttcttcg aatggagttc ggaaagaatc tccatattta tgaaaagcta
      121 gttgtccttt gggtttttgt ccatagccca agccttctgg cattagcagg ttttttaccc
      181 attctgtttt atcgtcttgg ttgacagtaa ttttagataa tgaattataa aaggcaaatt
      241 tttcgatgtt tgcaaaaaaa gtttttataa agctgtcgct ttctgccaac tgttcaacgt
      301 tttcggcatt agcagctttt tcaaggaaac tgtaaagatt tttgaacatg cggcttgcag
      361 ctcccgatgc cggaacaaac tttaaagcca ataggccaga ggaaagcgaa ttttcaaata
      421 ctgaaatcat tcgggttatg tcttcctccg ataggatttt aattccattt tcgggagtgg
      481 cggggcctat tagcttgatt ggcgcaaatc ctttttgaaa atgttggatc tgtttttcaa
      541 gctcttcaag ggtaagtccc tgtgtctcta tctgtttgat gtcgttttct gttaacataa
      601 gaaatttata atcaatgcgt taaaagtaaa aaacttcacc tgataaaaaa atcataatag
      661 gtacattcat taaaaaatcg aacttttttt attttgtatt ttcacccaat tttgtaatct
      721 aattcattct ttacttttac aggcgtgaca aaagtaacaa aaatatatgc ttggggacat
      781 cagaaacggt acaatgattt ctcgaatcac tttaaaattc tctttaaaga aagaattcag
      841 aaggtttcgg ttgatgccgg cttcacatgt ccgaatcgcg acggccttaa agggataggg
      901 gggtgtactt tctgtaataa caaaacgttc aatccttcct attgccaact ctcgaagagt
      961 attagtgccc agcttggaga gggaatctct tttttccgcc gaaaatacga ttcgatgaag
     1021 tttttggctt attttcaggc ttataccaat acatatgcgc ctgttgccga tttggagcga
     1081 atgtactcag aagcattgag cgtgcccgat gttatgggac ttgtggtggc tacgcgcccc
     1141 gattgtttga gtaacgatgt acttgatttg cttcaggctt ttgctgaaaa gtattacgtg
     1201 atggtagaac ttggggttga gtcgtgcgag aatgataccc ttgcacgtat taacaggggg
     1261 cacactttcg aagaatctgt tgccgccatt gaggcgttag cggctcgatc tattcataat
     1321 tgtgcgcatc ttattttagg gctgccgggc gaaaacaggt cagttatcct taaccaggcg
     1381 cgtaagatat ctgcactgcc agttgagaat ataaagttgc accagcttca gattcatcga
     1441 gatacagtta tggctcatca atatgttact catcccgggc agtttgatat ttatgattca
     1501 gcagccgatt ttgttgagct tattgttgac tacgtagagt tgttgcgccc ttcaattgtt
     1561 atcgaacgtt ttgtcagcga atcgccggct aaattgctta ttgctcccga ttggggtatt
     1621 aaaaattttg agtttgtagc taaagttgaa aagcgccttg ccgagcgcga tacgtggcaa
     1681 ggtcgtttat atcagccata aaaaaagcag ataagatttt acctgctttt ccttttaatt
     1741 ttctgaattt tttagtctgt tatatctcca ttaatgccca gtaaatcttg gtagaatggt
     1801 attagctggt cggccatttg catatggtcg tcaatgtcgg ggtggtaatc gcaaccgtgt
     1861 ggcacaatgc catcaaagct aaaaatagcg attggcttgt atttggttct tgaaaaatgg
     1921 ttcttcacgt tgttaaggca ttttaacagc agttctgatt tatcgcccga aagcattggg
     1981 ctcgagagta atgcaatctg tgtgtcagga tagtgattgt ataccgtttc aacaaattta
     2041 atataagttg aagtgaaaat ttcggcatcg aacgggtgtc gttcatgaat tccgtctcca
     2101 tctgaaaaat cattggttcc caggcatata cttactattt caggttcaat ttgtgagaaa
     2161 tcccattttt ttgaattatc agtgttgaga taacggtttt cgtacaccat aggcatggtt
//
LOCUS       DAOPUW010000310         2213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_52603_length_2213_cov_2.772938, whole genome shotgun sequence.
ACCESSION   DAOPUW010000310 DAOPUW010000000
VERSION     DAOPUW010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52603_length_2213_cov_2.772938"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..>2213
                     /locus_tag="PKJ43_05990"
     CDS             108..>2213
                     /locus_tag="PKJ43_05990"
                     /inference="COORDINATES: protein motif:HMM:NF026036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNZ72153.1"
                     /translation="MKNISNNIAQDIFPMWAGDEIFFLSDRDRIMNIFVYNTKTQQTS
                     KVTNFDEFDCKFPSIGGNYIVFENGGYIYKMNVNDKKPEKVSITITGDFNYARNETKK
                     SSPLIRNADLSPNGERVVFSARGEIFNVPSKKGISYNLTQTSGAHERDGIWSPDGKYI
                     AYLSDQSGEFEIWVQPHDKSAPARQVTKNADTYYFNILWSPDSKKILWNDRKLRLSYV
                     NVETGEVTEVYKARYGQTSDYAWSPDSKWITFNRPSENRFSTISIYNTVNKEVKDITN
                     NWYDSGSPNFSADGKYLVFVSDRDFSPTYSQTEWNHAYENMGKVYLAVLSEKTSSPFA
                     LENDSVAISTETLPEKSEKTKGKKEAKTPSQSNQKETLETEINFDGIGNRIISLPIEA
                     SNYSNVYCVDGKVYYNQSTDNGTKAKVYLLNKKEEKELGSFNFAISANNKKMLIREKT
                     SWGVIDLPSDQIKLSETIDIDKMEAWVDYAQEWKQIYDESWRQMRDFFYVENMHGVNW
                     KAMHEKYAPLVKHVRHRDDLTYIIGELIGELNVGHAYVQSGERPAPERVKTGLLGAKI
                     SRDISGYFKIDQLLKGANWSSSLRSPLTEIGVNAHEGDYIVSVNGTSTNTVSDIFSLL
                     AGKAETEVEIGLNNKPSETDARKIIIKPIADESALYYYNWVQGNIEKVNKASNGDVGY
                     IHIPDMSAEGLNEFAKYFYP"
BASE COUNT          763 a          452 c          477 g          521 t
ORIGIN      
        1 ggtaaacaac tcgcatttaa cagggtattt cgcgaattcc gcacctggaa atattacaga
       61 ggaggcatgg ccgatgatat ctggatttcg acttcgacac aaaagaagtg aaaaacataa
      121 gcaacaatat tgcacaggac atcttcccca tgtgggccgg cgacgagata ttctttcttt
      181 cggatcgtga ccgcattatg aacatttttg tgtataacac caagacacaa caaaccagca
      241 aagtaaccaa cttcgatgaa ttcgactgca aatttccttc tataggaggc aattatattg
      301 ttttcgaaaa tggcggctac atctataaaa tgaatgtaaa cgataaaaag ccagaaaaag
      361 taagtataac tattactggc gatttcaatt atgctcgaaa cgaaacaaaa aaatcaagtc
      421 ccctgatcag gaatgccgac ctgtctccca atggagaaag agtggttttc agtgctcgtg
      481 gcgaaatttt taatgttccc tctaaaaaag ggattagcta taatcttaca caaacttcgg
      541 gagcacacga acgcgatggc atatggtcgc ccgatggcaa atatatcgcc tatctttcag
      601 accagtccgg cgaatttgaa atatgggtgc agccccacga caaatcggct ccggcccgtc
      661 aggttactaa aaatgccgat acctattatt tcaatatctt gtggtcgccc gacagcaaaa
      721 aaatactatg gaacgaccga aagctacgcc ttagctatgt taatgttgaa acaggagaag
      781 taaccgaggt atacaaagcc cgatacgggc aaacctcaga ttatgcgtgg tcgcccgaca
      841 gcaaatggat aacattcaac cggccgagtg aaaacagatt ctcaacaatt agcatttata
      901 acacagttaa taaagaagtt aaagacatca ccaataattg gtacgactct ggatcgccca
      961 acttttcggc cgatggaaaa tatttagtct tcgtttctga tcgtgatttc tcaccaacat
     1021 acagtcaaac agaatggaac catgcctacg aaaatatggg caaagtttat ttggctgtgt
     1081 tgtcagaaaa aacgtcctct ccatttgccc ttgagaatga cagtgtagct attagcactg
     1141 aaacattacc agaaaaaagc gaaaaaacaa aaggaaaaaa agaagctaaa accccttctc
     1201 aatccaatca gaaagaaacg ttggaaaccg aaatcaattt tgatgggatc ggtaaccgaa
     1261 taatatcact tccgattgaa gccagtaatt actcgaatgt atattgtgtc gacgggaaag
     1321 tatattacaa ccaaagtacc gacaatggca caaaagccaa ggtatatctt ctgaacaaaa
     1381 aggaagagaa agaactgggc tcgttcaatt ttgcaatttc tgcaaataat aaaaaaatgc
     1441 tgatccgtga aaaaacaagc tggggagtta tcgacctgcc atcggaccag ataaaactga
     1501 gcgaaactat cgatatcgac aaaatggaag cttgggttga ttatgctcag gaatggaaac
     1561 agatttatga tgaaagctgg cgccagatgc gggatttctt ctatgtagag aacatgcatg
     1621 gggttaactg gaaagcaatg cacgaaaaat atgccccatt ggttaaacat gtgcggcacc
     1681 gcgacgacct cacctatatt attggcgaac tcattggaga actgaatgtt ggacatgcct
     1741 atgtacagag tggagaacgg cctgcgccag agagggttaa aacaggattg cttggagcca
     1801 agataagccg cgacatctcg ggatatttta aaatagatca actgctgaaa ggagcaaact
     1861 ggagcagttc gttacggtcg ccattgactg aaatcggggt aaacgctcac gagggcgatt
     1921 acattgtatc ggttaacgga accagcacaa atacagtcag cgatattttt tcacttctcg
     1981 cgggcaaagc cgaaactgag gtggaaattg gattgaacaa caaaccctca gaaactgatg
     2041 cccgaaagat aataatcaaa cctattgccg acgaatcagc gttatattat tacaattggg
     2101 tacagggcaa tattgaaaaa gtgaacaaag caagcaacgg agatgtagga tatattcaca
     2161 tacccgacat gagtgctgaa ggcttgaatg aatttgccaa gtacttctac ccg
//
LOCUS       DAOPUW010000311         2211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_52696_length_2211_cov_2.295455, whole genome shotgun sequence.
ACCESSION   DAOPUW010000311 DAOPUW010000000
VERSION     DAOPUW010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52696_length_2211_cov_2.295455"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /locus_tag="PKJ43_05995"
     CDS             complement(<1..1278)
                     /locus_tag="PKJ43_05995"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HNZ72154.1"
                     /translation="MKKMKSITILVVLFALTSYSFGAKEENFKFGKVSMEELNANICP
                     SDSNAHAYYIFDKGEAFYNYINGFQVKYKRHFRIKIIDKSGFDHATFAIPYYDSPSIK
                     EKIYSLKAFTYNVENGKIVKVELDKKNIFDEETTKNWKQKKFALPNVKEGSVIEVSYD
                     ITSDRYWSLNSIQFQYEIPVMVSDYSVEIPQYFTFNQAHRGSVMAKTTSKSGNQSLRT
                     AEGQSLQFSTTVYNYHIENVPAFPIGEELTTIDNYISKAEFELASVQIPGAVYKDYTT
                     TWADVNKIFLGDEDFGNRLKSTSFLNETANNIKQNIPTEHEQMRAALNFIQKKMKWNE
                     TNSCWCFTPLKKVFDSGVGSSADINMLLVALLREMGLYASPMVLSTRANGMIHPAVAS
                     INQMNYVIAACRIDGEVHLLDATDDYTDVDILPN"
     gene            complement(1472..>2211)
                     /locus_tag="PKJ43_06000"
     CDS             complement(1472..>2211)
                     /locus_tag="PKJ43_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HNZ72155.1"
                     /translation="AVASRALAKYNRELSAECLETALALWEKSGKNASSNDAKIEFAA
                     ELLISTGNQSFAEEIYKMEETVLRQANRIGWVIGKALPLLKNANFNEKMAQAIKSHAA
                     KVSDESKKSPFGVPYRPNIWGDGWTIQRFGVEQYFVHKAWPEITTPDYFIDALNFVLG
                     VHPGQNTASFASGVGSNSATVAYGVNRADWSYIPGGVVSGTAMIRPDLPEFKIWPYFW
                     QQTEYVMGGGSTNFMFLTLAVDKYFNE"
BASE COUNT          612 a          423 c          433 g          743 t
ORIGIN      
        1 gttgggcaaa atatctacat cggtatagtc atcggttgca tccaataaat gcacttcacc
       61 atcaattctg catgcggcaa taacgtaatt catttggtta attgaagcta cagccgggtg
      121 aatcatgccg tttgctcggg tgctgagtac cattggcgat gcgtaaagcc ccatttctct
      181 cagcaaagca actagtagca tgttaatatc tgccgaactt ccgacacccg aatcaaatac
      241 ttttttcagt ggggtaaagc accagcagct gtttgtttca ttccatttca tttttttctg
      301 aatgaaattt aaggcggctc tcatttgttc atgctcagtg ggtatgtttt gcttgatatt
      361 attggctgtt tcatttaaaa aagaagttga ttttaaacgg ttgccaaaat cttcatcacc
      421 gagaaatatt ttatttacat cggcccatgt cgttgtataa tctttgtaca ctgcccctgg
      481 aatttgaaca gaagcgagct caaactcagc tttagaaata taattatcta ttgttgtaag
      541 ttcttcgcca atagggaaag caggaacatt ttcaatgtga taattgtaaa cagtagtact
      601 aaactgaagg ctttgcccct cggcggttcg caatgactgg ttgcctgatt tgctggtggt
      661 ttttgccatt acagatcctc ggtgggcttg gttgaaggtg aaatattggg gaatttctac
      721 agaataatca ctgaccatta ccgggatttc gtattgaaat tgtattgaat taagcgacca
      781 ataccgatct gaagtaatat cgtagctaac ttctattacc gagccttcct tgacgttggg
      841 aagggcaaac ttcttttgtt tccagttttt agttgtctct tcatcgaaga tatttttctt
      901 gtcgagttca acttttacaa ttttgccatt ttcaacattg taagtaaatg ctttaaggct
      961 gtatatcttt tcttttatcg atggtgaatc gtagtacggg atggcaaagg ttgcatgatc
     1021 aaaacctgat ttgtcaataa ttttaatcct gaagtgtcgt ttgtatttga cctgaaagcc
     1081 attgatgtaa ttataaaatg cttctccctt gtcgaaaata taatatgcat gggcatttga
     1141 gtcggatgga caaatgttgg cattaagttc ttccatcgaa acttttccga acttgaaatt
     1201 ttcttctttt gccccaaatg agtatgaggt caatgcaaat aaaactacta gaatggtaat
     1261 ggacttcatt tttttcatgg tgaaggtttt taatttggtt tgattaagat ctttaaacga
     1321 caaaaacaac gtttatatta caaacatagt aattattctt aattattatg tttcgatttt
     1381 attgttttct gaaaggtaag ttcttaaaca cgctcctaaa aaaataggct gtcaatttat
     1441 tttgacagcc tattcgttaa gatattttat tctactcgtt gaaatacttg tcaacggcca
     1501 atgtaagaaa cataaaatta gttgatcctc cgcccattac atattctgtt tgttgccaga
     1561 aatatggcca tattttaaac tctggaagat caggtcggat catggcagtg cccgacacaa
     1621 cccctcctgg aatatatgac caatctgcgc gatttacccc ataggctaca gtggcagagt
     1681 tagaaccaac tcccgacgca aatgaagctg tattttggcc cgggtgaaca cccaatacaa
     1741 agtttaatgc atcgataaaa tagtcgggag tagtgatttc gggccaggct ttgtgcacaa
     1801 aatattgttc gactccaaag cgttgaatgg tccacccgtc gccccaaatg tttggacgat
     1861 aagggactcc aaatggagat tttttcgatt cgtcgcttac ttttgctgca tggcttttaa
     1921 ttgcttgagc cattttttca ttgaagtttg cgtttttcaa taagggcaat gctttgccga
     1981 tgacccatcc aatacggttg gcttgcctca atactgtttc ttccattttg tagatttctt
     2041 cagcaaaaga ttggttcccg gtagaaataa gtaattcggc agcaaattca atttttgcat
     2101 cgtttgatga agcgtttttc cccgattttt cccaaagggc taaagcagtt tcaaggcatt
     2161 cagccgataa ttcccggtta tatttagcca aagctcgcga agctacagcc a
//
LOCUS       DAOPUW010000312         2210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_52733_length_2210_cov_2.905800, whole genome shotgun sequence.
ACCESSION   DAOPUW010000312 DAOPUW010000000
VERSION     DAOPUW010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52733_length_2210_cov_2.905800"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..649
                     /locus_tag="PKJ43_06005"
     CDS             200..649
                     /locus_tag="PKJ43_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72156.1"
                     /translation="MRIILSLKNIFYLFVFLAFILVSCEDREEYIPYVEVSFSVDLNI
                     NNNLATPGYSMLYPGEGFGGVIVYCEYYDYVTPDKSIFKAYDAACTLEVEDSCSVTNT
                     GNSFYGECPCCHSQYEFTNGQPVEGDAAYSLKRYKATVISNKVYVRN"
     gene            complement(662..1168)
                     /locus_tag="PKJ43_06010"
     CDS             complement(662..1168)
                     /locus_tag="PKJ43_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNZ72157.1"
                     /translation="MSRLSGEAYKQATKNDVVLVLDNIRSSNNIGSFFRTADALLVQS
                     IYLCGYTATPPSKDIHKTALGAEDVVDWVYFENAKDAVMHLGEKGYTVYAVEQVENSI
                     LLHQFHPASDAKIALVFGNEVSGVQQEVINCCHGAIEIPQFGTKHSFNVSVSAGIVIW
                     DVVSKMKL"
     gene            complement(1199..>2210)
                     /locus_tag="PKJ43_06015"
     CDS             complement(1199..>2210)
                     /locus_tag="PKJ43_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HNZ72158.1"
                     /translation="QLLSKICDCEGTPYLFDYLLSQSADETTRLLLEGVDITRDNLTR
                     FLSAERYSLAPLHNFFFTRDASMSIFDNVLIAKMANSIRFRESIIMDAIFRHHPLFSV
                     ETINPSETVHRTLLPQLTIEGGDVLVLSDHVLLVGTGSRTSTHGVDFIIEELKKRMQP
                     KQFIIVQELPHSPESFIHLDMVFTVLDQEHCMIYKPLILGQNRYRTILISITYDKVES
                     ISIEQNIPEALGKIGYHFNMVYCGGGDDWNQEREQWHSGANFFALSPGIVVGYERNVH
                     TIEELSKHGFDVVTAKNIIGNKVGLPTSKTVITIPGAELARGGGGARCMSMPVKRKRV
                     DW"
BASE COUNT          657 a          485 c          418 g          650 t
ORIGIN      
        1 ttcgagccaa ttaaaaatat cctcatcacg aactcgttct gaaggggtat ttgcctgacg
       61 attgatcact ggcggtttct tttttttgcc cagtgcacca attattgata tggctattac
      121 gataattaga ggaattaaat cttccataat ttcccaataa taagttagtt ttgtaacaac
      181 gaaataaaat tacacaaaaa tgaggattat cctatcgctt aaaaatatat tttacctttt
      241 tgtctttctt gcctttattc ttgtttcgtg tgaagaccgt gaagagtaca ttccttatgt
      301 agaagttagc ttttcggtcg atttgaatat taacaacaat ttggccaccc ctggctattc
      361 aatgctctat ccaggtgaag gatttggcgg agtaattgtg tactgcgagt actacgatta
      421 tgtaacccct gacaaatcaa tattcaaagc ttacgatgct gcctgcaccc tcgaagtgga
      481 agacagttgc tctgtaacaa acaccggcaa cagtttctac ggcgaatgcc cgtgctgtca
      541 ttctcaatat gaatttacaa acggacagcc cgtggaaggc gatgccgcgt attcgctaaa
      601 aagatataag gctacggtaa tcagcaacaa ggtctatgtg cggaattgac tgcaacttac
      661 attataattt cattttcgaa acaacgtccc aaatgacaat tcctgcactt accgacacat
      721 tgaaagaatg ttttgttcca aattggggaa tctcaatagc gccatggcaa caattaatta
      781 cctcttgctg aacccctgac acttcatttc caaacaccag ggcaatttta gcatcagatg
      841 ccggatggaa ctgatgcaaa agaatactat tttctacttg ctcaacggca taaacagtgt
      901 accccttttc tcccaagtgc atcactgcat ccttcgcatt ttcaaagtaa acccaatcga
      961 caacatcttc ggcaccgagg gctgttttgt ggatatcttt tgaaggcggg gtagctgtat
     1021 acccgcataa atatatcgac tgaacaagca atgcatcggc tgttctgaaa aacgaaccga
     1081 tattgttact gctccttatg ttatcaagaa caagcactac atcgtttttt gtggcttgtt
     1141 tatatgcctc gccactaaga cgggacattt cgctattttt taatttacgc ataatcttct
     1201 accaatcaac tcgttttctt ttaacaggca tactcatgca acgggcacct cccccgcccc
     1261 gtgccaattc agctccagga attgtaatca ctgtctttga agttggcagc ccaaccttgt
     1321 ttccaataat attttttgcg gtaacaacat cgaagccatg ttttgaaagt tcttcaattg
     1381 tatgaacatt acgctcgtaa cccacaacaa tacccggaga aagtgcgaag aaattcgctc
     1441 cgctgtgcca ctgttcacgc tcctgattcc aatcgtcgcc tcctccgcaa tataccatat
     1501 taaaatggta accgattttt ccgagtgctt ccggaatatt ctgttctata ctaatggatt
     1561 caaccttatc ataagtaatc gaaattagta ttgtacggta cctgttttgc ccaagaatca
     1621 agggtttata aatcatacaa tgttcttgat ccaatacagt aaaaaccata tcgagatgaa
     1681 tgaacgactc gggagaatga ggaagttcct gaacaataat aaactgcttt ggctgcatac
     1741 gttttttcaa ctcttcaatt ataaaatcaa ccccgtgtgt cgaagtgcgg cttcctgtcc
     1801 ccacaagtaa aacatggtcg ctgagcacca gtacgtcccc gccttcaatg gttagctgtg
     1861 gcaataaagt acgatgtact gtctctgatg gattaattgt ttctacagaa aagagtggat
     1921 gatgcctgaa aattgcatcc ataattattg actcacgaaa acgaatgctg ttagccatct
     1981 ttgcaattaa tacattatcg aaaatcgaca tggaagcatc gcgggtaaaa aagaaattat
     2041 gtaatggagc caggctataa cgttcagccg ataggaagcg ggtaaggttg tcacgggtaa
     2101 tgtcgacccc ctcgagcaat aagcgggttg tttcatccgc agattgagat aaaaggtaat
     2161 cgaaaagata aggtgtacct tcacaatcgc atattttaga taaaagttgc
//
LOCUS       DAOPUW010000313         2207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_52835_length_2207_cov_3.475836, whole genome shotgun sequence.
ACCESSION   DAOPUW010000313 DAOPUW010000000
VERSION     DAOPUW010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52835_length_2207_cov_3.475836"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            429..1742
                     /locus_tag="PKJ43_06020"
     CDS             429..1742
                     /locus_tag="PKJ43_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HNZ72159.1"
                     /translation="MRPTLIILLLALTIGALGQNTEKKDCNKDVSDSRGKMIPWEQGA
                     HKTGKYRNVFLDAGYSKADIDAKLAKAYYDVFEGPNRVYFEVGDSMGYVSDVKNQDAR
                     TEGLSYGLMVAVQLNKKDVFDRLWRWTKKYMQHQSGPREAYFAWSVDPKTGNQLSGGS
                     ASDGELYFITSLLFASNRWGNDTGIDYHAEAKRILDAMWSKDGTNGITNVINVEHKLI
                     TFVPDRGGYNYTDPSYHLPAFYEVWAEYGKDGREQFYRDCADSARAYLHRAIHPETGL
                     NPDYSEFSGKPFVRWRMGDAFRFDSWRVPMNIAMDYSWYGKDKEWQKEYAVKIQNFLF
                     CQGINTFVDQYTVDGKTPEWILPAGKPGVQKLRHSLGLVSTSAAASIMGTQAKSWKFI
                     DELWNAKLEPYEDGYFDPYYDGLLYLFSIMHLSGNYQIIKPANAK"
     gene            complement(1782..>2207)
                     /locus_tag="PKJ43_06025"
     CDS             complement(1782..>2207)
                     /locus_tag="PKJ43_06025"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HNZ72160.1"
                     /translation="KKEKFKFSSIMSQYKSAYTKPPVIDHDLWMTNYLGHPYQGAFYY
                     NTVRCQGASVLQSSLFCIGHSLFWEYGWEAGIEQPSIQDMITTPLGGIIVGELAHVAT
                     ISMSRNGFKWYEIVAVCAINPSYALNNGFRFNKPLKIKN"
BASE COUNT          688 a          470 c          458 g          591 t
ORIGIN      
        1 cccaggtagg acttgaacct acgacctacg gattaacagt ccgccgctct aaccaactga
       61 gctactaggg agtatctgat tcatttcaat aatttcgata acactgaatc gcttgccatc
      121 gaaataaccc gattttcatc ggagctgctt tatcccgact tgcaacggga ttcaacaaag
      181 tcaccatggt tttgaacgac tgccttgttt caaaagcgat gcaaaaatag aaaagatttt
      241 attcctgcaa aagttttcaa gaaaatttat cttcaaaaaa acatcttttc agtttttcca
      301 tgattagtgg gctttcattg cctattttta taaagaacgg atgaatattt acacaagaga
      361 ataaaccatt ttcaagaatt cacatcaaaa taaaaatagt atattttgga aaatttagat
      421 ttgtgaacat gagaccaaca ctgattattt tactactggc cctaaccata ggagcattgg
      481 gccaaaacac cgagaaaaag gattgtaata aagatgttag cgacagccga ggcaaaatga
      541 tcccatggga acaaggtgcc cacaaaacgg gcaagtaccg caacgtattt ctggatgccg
      601 gatatagtaa ggccgacatt gatgcgaaac tggcaaaagc atactacgat gtgtttgaag
      661 gaccaaacag ggtgtatttt gaagttggcg attcaatggg ttatgtttct gatgtcaaaa
      721 atcaggatgc ccggaccgaa ggcctttcat atggcctcat ggttgccgtt cagcttaaca
      781 agaaagatgt ttttgaccgt ctttggcgat ggacaaaaaa atatatgcaa caccaaagcg
      841 ggccacgcga agcttatttt gcatggagtg tcgaccctaa aaccggaaat caattatccg
      901 gaggttcagc ttcagatggt gaactctatt ttattaccag cctgctcttc gcatcaaacc
      961 gctggggcaa cgataccgga atcgattatc atgccgaagc caaaagaata ctcgatgcca
     1021 tgtggagtaa agatggcacc aacgggatta ccaatgtgat taatgtagaa cataaattga
     1081 tcaccttcgt tcccgacaga ggcggatata actatacaga cccatcgtac cacttacctg
     1141 ctttttatga agtgtgggca gaatatggca aagatggccg cgaacaattc taccgcgatt
     1201 gtgctgactc agctcgcgca tacttgcacc gggcaattca ccctgaaaca ggtttaaacc
     1261 ctgattactc agaatttagc ggcaaaccct ttgtccgctg gagaatgggt gatgctttcc
     1321 gtttcgactc atggagggta cctatgaaca tcgccatgga ttattcatgg tatggaaaag
     1381 ataaagagtg gcaaaaagaa tatgcagtaa aaatccagaa ttttcttttc tgtcagggca
     1441 ttaacacttt tgtagatcaa tatacggttg atgggaaaac ccccgaatgg atacttcctg
     1501 cagggaaacc cggtgtgcag aaattgcgcc actcactggg cttggtttcc acttcggcag
     1561 ccgcgtcaat tatgggaaca caagccaaaa gctggaaatt tatcgatgaa ttgtggaatg
     1621 ctaaacttga accatatgaa gatggatact tcgacccgta ttacgacggt ctactttatc
     1681 ttttcagcat aatgcacctc agcgggaact atcagatcat caaacctgca aatgcaaaat
     1741 aacaaaaaca aattagagcc agtttctgaa aagttactgg cttaattttt tattttcaat
     1801 ggtttattaa aacgaaaccc gttattcaac gcatacgatg ggtttattgc acaaacggca
     1861 acaatctcgt accatttgaa accattcctg ctcatcgaga tagtggcaac atgagccaat
     1921 tcgccaacta ttatgccacc cagcggtgta gtaatcatgt cttgtatgct gggctgctct
     1981 ataccggctt cccagccata ttcccaaaac aatgaatgcc caatgcagaa aagcgacgat
     2041 tgcagcaccg atgctccctg gcatctgaca gtgttataat agaatgcccc ttggtacgga
     2101 tgtcccagat aattggtcat ccataaatcg tgatcgataa caggaggctt tgtgtaagca
     2161 cttttatact gggacataat tgaagaaaat ttgaattttt ccttttt
//
LOCUS       DAOPUW010000314         2205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_52901_length_2205_cov_3.337674, whole genome shotgun sequence.
ACCESSION   DAOPUW010000314 DAOPUW010000000
VERSION     DAOPUW010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52901_length_2205_cov_3.337674"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PKJ43_06030"
     CDS             <1..773
                     /locus_tag="PKJ43_06030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ72161.1"
                     /translation="YLFSTVKKQKAKLSEQNLELDRLNKTLNRLFAIISHDLRNATAA
                     YQSSAKVIQHHLERGQPEKLLPIAAEINANAGSLSTMLENLLQWSVIQMKGLSPRREI
                     LPIAEEVEKITSLYKTDAEKKGNNITIDVAPDHEIYCDRESFSLILRNLIGNANKFTS
                     NGEITIASKKEGQSMILIVRDTGCGMTEKMKNEIFSNHKENVRNGTSGEKGTGLGLIM
                     VKEHVEKNGGSIVVESNSEADQGGNGTTFVLHLPSEHI"
     gene            851..1561
                     /locus_tag="PKJ43_06035"
     CDS             851..1561
                     /locus_tag="PKJ43_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ72162.1"
                     /translation="MKVFIVEDELIHLEAIKIAIEEAGLELVGECNNADLAFDLIKKA
                     APDVVLVDIALPGLLNGITLAAKIYDELAIPHIFTTSFTQDEVIKQAVATKPAGYLRK
                     PVDPVTLKAAVQIAVSGQSQNKNETDSLSSKTVFAKIGDKLVRINIDEILMVKADGEN
                     CISLVLEKKEISCRTTLKEFCKQLPINFIQVHRGYVINIDHLDSFNEREQTAHLNNHT
                     APVARNFRKDFLNSIRKI"
     gene            1808..2164
                     /locus_tag="PKJ43_06040"
     CDS             1808..2164
                     /locus_tag="PKJ43_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72163.1"
                     /translation="MQRVIILLIFFFVSTNCSGTISLKGSVWQIDSISTSNYLFKEGM
                     KVDFAVNKIRLQEGEQTFAYPVIVSDNRLVIETGPVKWLFEIERTSDTAIILHELYSK
                     KPLEITLVKIKNKDKS"
BASE COUNT          726 a          428 c          436 g          615 t
ORIGIN      
        1 tctacttgtt ttcaaccgtt aaaaagcaaa aggcaaaatt atcggaacaa aaccttgaac
       61 tcgacagact gaataaaacg ttgaaccgcc tgtttgccat tatttcacac gacttgcgaa
      121 atgccaccgc agcctatcaa agtagcgcca aggtaattca gcaccatctt gagagagggc
      181 agcccgaaaa gcttttgcca attgcagctg aaataaatgc caatgccggc agcctttcta
      241 cgatgcttga aaatctgttg cagtggtcgg taattcagat gaaaggctta tctccacgac
      301 gagagattct tccgattgct gaagaagttg aaaaaataac ttctctgtat aaaacagatg
      361 cggaaaagaa aggaaacaac atcaccattg atgtggcacc cgatcatgaa atttattgcg
      421 atagggagag cttttcactc attctgagaa acctgattgg caatgccaac aagtttactt
      481 caaacggtga aatcaccatt gcatcgaaaa aagaagggca aagcatgatt ctcattgttc
      541 gcgatactgg ctgtggaatg accgaaaaga tgaagaatga aatttttagc aaccataaag
      601 agaatgtccg taacggtact tcgggcgaaa aaggtacagg attggggctg atcatggtaa
      661 aagaacatgt ggaaaaaaat ggaggttcca ttgttgttga aagcaactcc gaagctgatc
      721 agggagggaa tgggacaacg tttgttcttc accttccttc ggaacatatt tgaaaagatg
      781 ctggaaaaat gctagatatt cgaaactgga tttggaattt gtgatttgga atttgtgatt
      841 tggaattttt atgaaggtat ttatagttga agatgaactg atccaccttg aggcaataaa
      901 gattgctata gaagaggcgg ggttggaatt ggtcggtgag tgcaataatg ctgaccttgc
      961 tttcgacctg ataaaaaaag cagcaccgga tgtggtttta gtagatattg ctttgcctgg
     1021 tttgctaaac gggattaccc ttgctgctaa aatttacgat gaattagcca ttcctcatat
     1081 ctttaccacc tccttcaccc aagatgaagt tatcaaacag gctgttgcaa caaaaccagc
     1141 cgggtacttg cgaaaaccgg tagaccctgt aaccctgaaa gctgctgttc aaatagctgt
     1201 gagcggtcaa tctcagaata agaatgaaac cgattctctc tcttcaaaaa ctgtttttgc
     1261 taaaattggt gacaaactgg tacgcataaa tattgatgag attttgatgg tgaaagccga
     1321 tggcgagaac tgcatttcac tggtacttga aaagaaagaa atatcgtgcc gcaccacctt
     1381 aaaagaattc tgcaaacaac ttccaatcaa ttttatacag gtacaccgcg ggtatgtgat
     1441 caacatagat cacctcgaca gctttaacga aagggaacaa acagcccact taaacaatca
     1501 tactgcgcct gttgcccgca atttcagaaa agatttttta aactcgatca gaaagattta
     1561 atcccatttc ttcttcttta ttctgatggt gatatttgca ttttcgtaat catcaaacag
     1621 aaaaatcatc attcgaaaat aatgctgcac aaatacatat tcgttaaaaa cagaatgtgt
     1681 attcattaga ataatttcct tttagcctgc aaatattacc tcttaacact caaatccttc
     1741 ctcccggata caatgccggg caatccgttt tggcctattc taaatttgaa agaaaaaagc
     1801 ccggattatg caacgagtta ttatccttct gatattcttt tttgtttcta ctaattgcag
     1861 tggaacaatc agtctgaagg gctctgtatg gcaaatcgat tccatctcaa catcaaacta
     1921 ccttttcaag gaagggatga aggttgattt cgctgtcaat aagattcgtt tacaagaagg
     1981 cgaacaaacg tttgcttacc ctgtgattgt ttccgataac cgtttggtaa ttgaaaccgg
     2041 ccctgtaaaa tggctttttg aaattgaacg aacctcagat acagccatta ttctacacga
     2101 attatattcg aagaagccac tcgaaatcac attggtaaaa atcaaaaaca aagataaatc
     2161 gtaataaaat ctaaaatcat gaaaagcatt tactctcttt tcatt
//
LOCUS       DAOPUW010000315         2203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_52991_length_2203_cov_2.620577, whole genome shotgun sequence.
ACCESSION   DAOPUW010000315 DAOPUW010000000
VERSION     DAOPUW010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52991_length_2203_cov_2.620577"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..665
                     /locus_tag="PKJ43_06045"
     CDS             63..665
                     /locus_tag="PKJ43_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNZ72164.1"
                     /translation="MFADGVLPLVESGVINGANKAIDKGKMVSTFLMGSQKVYDFIHN
                     NPQVAMMDVGYTNDPFIIAKNPQVTAINSALSVDLTGQVNADSLGIKHFSGVGGQVDF
                     VYGASRSKGGKAIIAMPSVTNKGVSKISPVLMSGAGVVTTRNHIHWFVTEHGSVNLYG
                     KTLQERAKLIISIAHPDHREALEQAAFERFGPHFRFVKNF"
     gene            888..1289
                     /gene="mce"
                     /locus_tag="PKJ43_06050"
     CDS             888..1289
                     /gene="mce"
                     /locus_tag="PKJ43_06050"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNZ72165.1"
                     /translation="MSITHIEHIGIAVSNLDEAIAYYEKVLGLSCYAIEEVTDQKVKT
                     AFFKVGETKIELLESTDPEGPIGKFIEKKGQGVHHIAFAVSEVNEALKEAESNGVQLI
                     DKAGRKGAEGLNIGFLHPKSTLGVLTEFCSK"
     gene            1299..>2203
                     /locus_tag="PKJ43_06055"
     CDS             1299..>2203
                     /locus_tag="PKJ43_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNZ72166.1"
                     /translation="MNNQDKIKNLIEKRVQAKMGGGEKRIAAQHEKGKLTARERIELL
                     LDEGSFEEYDMFITHRCDNFGIDKNKPLGDGVVTGQGTIDGRVVYVFAQDFTVFGGSL
                     SETFAQKICKVMDMALKVGAPLIGINDSGGARIQEGVTALAGYAEIFERNIMASGVIP
                     QISAIFGPCAGGAVYSPALTDFIVMSKDTSYMFLTGPKVVKTVTGEVVTDEQLGGASV
                     HGSKSGVSHFIAEDEREGILLIRKLLSYLPQNNLEDAPLAECDDPIDRLDDFLNEIIP
                     DNPNKPYDVKDVIHSIVDYNEFLEV"
BASE COUNT          701 a          457 c          470 g          575 t
ORIGIN      
        1 ggtctccacc ctgaccgtcc ttccgacgca cctcttccag tcgttctcgg attcacaccg
       61 aaatgtttgc cgatggtgtt ttgcctctgg tagaaagcgg cgtaatcaac ggagcaaaca
      121 aggccataga caaaggcaaa atggtttcaa ccttcctgat gggatcacaa aaagtatatg
      181 actttattca caataaccca caagttgcca tgatggatgt tggctatacc aacgatccgt
      241 tcattattgc aaagaaccct caggtaactg caatcaattc agctctttcg gtcgatctca
      301 ccggtcaggt taatgccgat tcactgggaa taaaacattt ctcaggcgtt ggcggtcagg
      361 tagacttcgt atatggagct tcacgttcga aaggcgggaa agcgattatt gccatgccat
      421 cggttaccaa taaaggagtt agtaaaattt caccggtcct tatgtcggga gcaggtgttg
      481 ttaccacacg caaccatatc cactggtttg taactgaaca cggttcggta aacctttatg
      541 gaaaaacact tcaggaacgt gcaaaactga ttatctcaat tgcacatcct gatcatcgcg
      601 aagctctcga acaagcagct ttcgaacggt ttggcccaca ctttagattt gttaaaaatt
      661 tctaacataa aaggcaggtt ctcctgcctt tttttatggc ttttatcgcg aactaattac
      721 acaataacaa acaatatatt aacaagttag ctctaaataa attccaaaaa acattcgaat
      781 gattcgttta tatgttttaa ttgaattact tttgcacggt gaagaaaaac aggaatattt
      841 atagtaaaca gataaataaa agatattaat ccaataaatt ttaactaatg agtattacac
      901 atattgaaca cattggaatt gcagtttcca atttagatga agcaattgcc tactacgaaa
      961 aagtattagg actcagctgc tatgccattg aagaagttac cgaccagaaa gtaaaaactg
     1021 cttttttcaa agtaggcgaa acaaaaattg aactgcttga atcgaccgac ccggaaggac
     1081 caatcggtaa attcattgaa aaaaagggac aaggagtaca ccacatcgcc tttgccgtaa
     1141 gcgaagtaaa tgaagcgttg aaagaagccg agtcgaacgg ggttcagctt attgacaaag
     1201 caggaagaaa aggagcagaa ggattgaaca ttggattcct tcacccaaaa tcgaccttag
     1261 gtgtattgac tgaattctgt tcaaaataaa tgcatgcaat gaataaccag gataaaataa
     1321 agaacctgat tgagaaacgc gttcaggcaa aaatgggcgg cggcgaaaaa cgcattgctg
     1381 cacaacacga gaaaggtaaa ctaaccgctc gcgagcgaat agaactttta ctcgacgaag
     1441 gaagttttga agaatacgat atgttcatca cccaccgatg tgacaatttc ggtattgata
     1501 aaaacaaacc actcggcgac ggagtagtca ccggacaagg cacgattgac ggtcgtgttg
     1561 tgtatgtttt tgcgcaagac ttcactgtat ttggaggctc attatctgaa acatttgcac
     1621 aaaagatatg caaagtcatg gatatggccc ttaaagttgg tgccccttta attggcatca
     1681 atgactcggg cggcgcacga atacaggaag gggttactgc attggccggt tacgccgaaa
     1741 ttttcgaacg taacataatg gcctccgggg ttattccaca gatttcagcc attttcggcc
     1801 cttgcgcagg cggagctgta tactctccag cgttgaccga ctttattgtg atgagcaaag
     1861 acaccagcta catgttcctt actggtccga aagttgtaaa aaccgttacc ggtgaagtgg
     1921 ttaccgacga acagcttggt ggagcttctg tacatggatc gaaatctggg gtttcgcatt
     1981 tcatcgctga agacgaacgg gaaggaatat tacttattcg caaattgtta agctatcttc
     2041 cacaaaataa ccttgaagat gcgccattgg cagaatgcga cgacccaatc gatcgtctcg
     2101 atgatttttt gaatgaaata attcccgaca atccgaataa accctacgac gtaaaagacg
     2161 ttatccattc tattgtggat tacaacgaat ttttggaagt aca
//
LOCUS       DAOPUW010000316         2203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_52993_length_2203_cov_2.513966, whole genome shotgun sequence.
ACCESSION   DAOPUW010000316 DAOPUW010000000
VERSION     DAOPUW010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52993_length_2203_cov_2.513966"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="PKJ43_06060"
     CDS             <1..919
                     /locus_tag="PKJ43_06060"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNZ72167.1"
                     /translation="KSKGLINISAPELRRFRIFYNVYPNLCSNFLNSLDTSSNRGMLS
                     HDLSFFQIRGTTSHESEKIVPQDKLLKSLSFSHFVELIKIEDQLKRTFYEIQAIEGTW
                     SVSELKRQIASLYFERSSLSLNKEQLNALANRDAQQIKPKDLLKDSFVFEFLGLPVKD
                     AVEEADLEDALIDNLQEFLLELGNGFCFEARQKRILIDDEYFFIDLVFYHRILKCHVL
                     IELKTESFNHKNIGQLQAYLEFYKENIMQKDDRPPVGILLCTESKPQMVRYALANSEN
                     LFVSQYRLQLPTEEELQRFLEDQLKRIIN"
     gene            921..1418
                     /locus_tag="PKJ43_06065"
     CDS             921..1418
                     /locus_tag="PKJ43_06065"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNZ72168.1"
                     /translation="MTHDNISIIVAIAQNFAIGKNNDLLFHLPNDLKRFKEITTGKPV
                     IMGRNTLLSLPGSRPLKNRRNIVISDIPNEIFEGCEMTDSIEGAIQLVKNEAEAFIIG
                     GGMIYRQFYPLAGKLYLTVVHKDFEADVYFAEIDYSEWEETSREDFFDEKNNFNYSYI
                     NLTRK"
     gene            1568..>2203
                     /locus_tag="PKJ43_06070"
     CDS             1568..>2203
                     /locus_tag="PKJ43_06070"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNZ72169.1"
                     /translation="MAGNNIKNSKGFIFTGMGSEENEYIPIIADGENDELQIDSMPTA
                     LPLLPLRNTVLFPGVILPITVGRHKSLKLIKDVYRRDGVLGTIAQIDAEVDEPSLSDL
                     YEIGTIAKILKILEMPDGSTTVIVQGKQRFRALELISDEPYHTVRFQALSEIQPEGNS
                     PEFEAIVESLKDIAIKIAKNSNNLPPEATFAVRNIENPVFLINFICSNADIK"
BASE COUNT          748 a          408 c          429 g          618 t
ORIGIN      
        1 aaagagcaaa ggattgatca acataagtgc gcccgaactt cgacgattta gaatttttta
       61 caacgtttat cctaatctct gcagtaattt tctaaactca ttggatacga gctcaaatcg
      121 tgggatgcta tcccacgatt tgagtttttt tcagattcgt gggacgacat cccacgaatc
      181 tgaaaagatt gtgccacaag acaaactatt gaagagttta tctttctctc attttgtcga
      241 acttataaaa attgaagatc agctgaaacg tacgttttac gaaattcaag caatcgaagg
      301 cacttggagt gtaagtgaat taaaaagaca aattgcttca ctttattttg aacgatcaag
      361 tttgtcgctt aataaagaac aattaaatgc attggcaaac cgagatgctc agcaaattaa
      421 acccaaagat ttattaaagg attcattcgt gtttgaattt ctaggtttgc cagttaaaga
      481 tgctgttgaa gaagctgatc tggaggatgc attgatagat aatttgcagg aatttcttct
      541 tgaactggga aacggatttt gtttcgaagc aaggcagaag cgcatactta ttgatgatga
      601 atatttcttt attgatttag tgttttatca tcgaattttg aaatgtcatg tgttgatcga
      661 attgaaaaca gagtcattta accacaaaaa tattgggcaa ttgcaggcct atctggaatt
      721 ttataaagaa aatattatgc agaaagatga ccgaccacct gtagggattc ttctttgcac
      781 cgaaagtaaa ccacaaatgg ttcgatacgc tcttgctaat tccgaaaacc tttttgttag
      841 tcaatatagg cttcagttgc caacagaaga agaactccaa cgttttcttg aagatcaact
      901 aaaaagaata attaattgaa atgacacacg acaacatatc catcattgtg gccatagcgc
      961 aaaactttgc cattggaaaa aacaacgact tgttgttcca cttgcccaac gacctgaaaa
     1021 gatttaagga gattaccact ggaaaacctg taatcatggg gcggaacacc cttttgtcgt
     1081 tgcccggtag ccgaccgtta aaaaatcgcc gcaacattgt aatatccgac attccaaacg
     1141 aaatctttga gggatgcgag atgaccgatt caattgaagg agctattcaa ctggttaaaa
     1201 atgaggcgga agctttcatt attggcggcg ggatgatcta ccggcagttt tatccactag
     1261 caggaaagtt gtaccttacc gttgtccata aagattttga agctgacgta tacttcgctg
     1321 aaattgatta tagtgaatgg gaagaaacca gcagagaaga ttttttcgac gaaaagaaca
     1381 acttcaacta ctcatatatc aatttaacgc gaaaataaaa gcctgaaata aattttattt
     1441 tctgttggtt cttggcaaaa tttagccata accaatattt ggcatagcta ttgacaaaca
     1501 cagaagctca taatttgtta tactttaata agacagtttg tcagcataaa aaaaattgaa
     1561 atacacgatg gcggggaaca acataaaaaa cagcaaaggt tttattttca caggaatggg
     1621 aagtgaagaa aacgaatata taccaattat tgccgatggc gagaacgatg aattgcagat
     1681 cgacagtatg cctactgccc tgccattatt accacttagg aatactgttc tctttcctgg
     1741 agttatttta cccattacgg ttggcaggca caaatcgcta aaactcatta aagatgtata
     1801 tcgtcgtgac ggggtacttg gaaccattgc ccaaattgat gcagaagttg atgaaccatc
     1861 gctaagcgac ctatacgaaa ttggaacaat tgccaagatt ctgaaaatac ttgaaatgcc
     1921 agatggaagc accactgtta tagttcaagg aaagcaaagg ttcagggcac tcgaactaat
     1981 atcggacgaa ccataccaca cggtgcgctt tcaggcgttg tccgaaattc aacctgaggg
     2041 aaattctcct gaatttgagg caattgtaga atcgctgaaa gatatcgcca taaaaattgc
     2101 aaaaaactcg aataacctac ccccagaagc aacattcgca gtaagaaaca tcgagaatcc
     2161 ggtctttctt atcaatttca tctgttcaaa tgccgacata aaa
//
LOCUS       DAOPUW010000317         2192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_53399_length_2192_cov_2.810950, whole genome shotgun sequence.
ACCESSION   DAOPUW010000317 DAOPUW010000000
VERSION     DAOPUW010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53399_length_2192_cov_2.810950"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PKJ43_06075"
     CDS             complement(<1..688)
                     /locus_tag="PKJ43_06075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNZ72170.1"
                     /translation="MTTNTLVAKIWSFCDTLRDDGLNYGDYLEQLTYLLFLKMADENK
                     AQYNIPVGYDWQSISDKNKTGGNLLEQYEEILKKLASSGGMLKSIFDGAQNKIHDSAK
                     LKKLINLIGEENWSSKEVDIKGEIYESLLQKNAENSGAGQYFTPRPVIKAMVNCIRPE
                     VGETIADPSCGTGGFFLGALEYLHTKTLSKKDQEEISFKTFHGWEIEKSTARLCLMNL
                     FLHGVGDLVNT"
     gene            complement(701..2134)
                     /locus_tag="PKJ43_06080"
     CDS             complement(701..2134)
                     /locus_tag="PKJ43_06080"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HNZ72171.1"
                     /translation="MKLFIYLNNCAMAINRIKNILSQGEGIRNEFKQTREKLPENLFE
                     TICAMLNRSGGDIILGADDNGNLTGIEAAMVAGMIANIANLSNNPQKLNPPFILFPKE
                     HVVDGFHVIHLQVPESSQVHQTTNKIYDCSNDGDFVVRGAHQIAELYNRKRNHYTEAT
                     IYPAFRFEDFKQPLFHKVRNLIRSYNPEHPWLELDDEQMLRMAGLYDRDYKTGQEGYT
                     LAAILLLGKDEAIRSVVPHYKIDALVRKFNVDRYDDRLYIQTNLIEAYEQLLEFVAKH
                     LPDKFYLEGDQRRSLRTIIFREVAANILVHKEYTNAYPTTFVITKSAVIAENANVPNG
                     EGPINVNNFKSYPKNPVIAKFFMQLGRFDELGSGVLNINKFCKAYSGQEHPEFIEGPI
                     FKTIIPLDEGLNEGLNEGLKLLLETIILNEGIQAKNLAEKLNRPLKTIERQIKELVQR
                     NLIERRGSKKSGGYFPLPTQKSEKTEE"
BASE COUNT          540 a          486 c          457 g          709 t
ORIGIN      
        1 gcgtattgac caagtcgcca acaccatgaa ggaaaaggtt catcaagcac aagcgggctg
       61 tcgatttttc aatttcccat ccatgaaatg ttttgaacga aatttcttct tgatcctttt
      121 tcgaaagtgt tttcgtgtga agatattcca aagcccccag aaagaatccg ccagttccgc
      181 aacttgggtc ggctattgtt tcacccactt cagggcgaat gcaattgacc attgctttaa
      241 ttaccggacg aggggtaaaa tattgaccag caccgctatt ttcagcattc ttctgcaaca
      301 gtgattcgta tatttctcct ttaatatcaa cctctttgct gctccagttc tcttcaccga
      361 tcaggttaat gagtttcttg agtttagccg aatcgtgtat cttattttga gctccgtcaa
      421 atattgattt taacatacca ccagaggatg ccagtttttt caggatttcc tcatattgtt
      481 ccaaaaggtt accacctgtt ttgtttttat cgctgatgct ttgccaatca taaccaaccg
      541 gaatattgta ttgtgcttta ttttcgtcgg ccattttcag gaacagcaaa taggtcaact
      601 gttccaggta atcgccatag tttaatccgt catccctgag ggtgtcgcaa aacgaccata
      661 tttttgctac cagtgtattg gttgtcattt tttcaattat ttattcttcc gttttttcac
      721 ttttttgagt tggcaaggga aaatacccac cactcttttt acttcctctc cgctcgataa
      781 ggttccgttg tacaagttct ttaatttgtc gttcaatggt ctttaacgga cggttaagct
      841 tttctgcaag gttcttagcc tgaatgcctt catttaagat aatagtctcc aataataatt
      901 ttaatccctc atttaatccc tcatttaatc cctcatcgag ggggataatg gttttaaaga
      961 tcggcccttc gataaactct ggatgttctt gaccagaata cgctttgcaa aacttgttaa
     1021 tattcaacac acccgacccc aattcgtcaa accttcccaa ttgcataaaa aacttggcaa
     1081 tgaccgggtt cttgggatac gatttaaagt tgttcacgtt aattggccct tcaccgttgg
     1141 gaacattggc attttcggca atcacagccg atttggtgat cacaaatgtg gttgggtaag
     1201 cattggtata ttctttatgt accaaaatat tagcagccac ctcacggaaa ataatggtgc
     1261 gaaggcttct ccgttgatca ccttccaaat aaaatttgtc gggcaggtgc ttggctacaa
     1321 attcaagtaa ttgttcgtag gcttcaatca ggttggtttg aatatagaga cggtcgtcgt
     1381 aacgatcgac attaaacttg cgaaccagtg catcgatttt ataatggggc actacgctgc
     1441 gaatggcttc atctttccca agcagcagaa tagccgccag ggtatagcct tcctgcccgg
     1501 ttttgtagtc acggtcgtac aacccggcca tgcgtagcat ctgttcatcg tccagctcca
     1561 gccagggatg ttcgggattg taactgcgta tgaggttgcg cactttgtgg aaaagcggtt
     1621 gcttgaaatc ttcgaaacgg aaggctgggt aaatggttgc ttcggtataa tggtttcgtt
     1681 tgcggttgta aagctcggct atttggtgtg caccgcgtac cacaaaatcg ccatcgttgc
     1741 tacagtcgta tattttattg gttgtttggt gtacctgtga gctttcggga acctgaaggt
     1801 gtataacatg aaagccatct accacatgct ctttggggaa aaggataaac ggtgggttta
     1861 gcttttgcgg gttattgctc aggttggcta tgttggctat catcccggca accatagcgg
     1921 cttctattcc ggtaaggttc ccgttatcgt cggcacccag aataatatcc cctccgctgc
     1981 ggttcaacat ggcacatatc gtttcaaaca ggttctcagg caacttttcc cgggtctgtt
     2041 taaactcatt gcgaatgcct tcgccctgag ataggatatt tttgatgcgg ttgatggcca
     2101 tagcacagtt atttaagtat atgaacagct tcacccagcc attcgtagtt tttttccaaa
     2161 gatttagttt tcacaaagct tgtaactacc at
//
LOCUS       DAOPUW010000318         2190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_53459_length_2190_cov_4.300703, whole genome shotgun sequence.
ACCESSION   DAOPUW010000318 DAOPUW010000000
VERSION     DAOPUW010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53459_length_2190_cov_4.300703"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PKJ43_06085"
     CDS             <1..418
                     /locus_tag="PKJ43_06085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ72172.1"
                     /translation="IVSPQIPRIFKKVKQEICNGELSTSSIFLSQLPHEQLINEILSS
                     HCVIIPSLCEGFGFTAVEACSMKIPLISSGQGSLPEVVSGNIITMDEYSPQGLTDAMQ
                     KAINGSFEHIEDKKFTVETFVENHKQLYYNILQNNK"
     gene            440..862
                     /locus_tag="PKJ43_06090"
     CDS             440..862
                     /locus_tag="PKJ43_06090"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNZ72173.1"
                     /translation="MATFKNKFPVQVRFSDVDVMGHVSNTVYQNYFDAGKLNYFEEVL
                     PEMDFVTIGIVGASIKIDYLKPIFMKTRILVETRVAQIGTKSITMEHCIIDEHSGELL
                     STCHAVMVCFDLKERQSIPIPEEWKKKIAAYEGDQLIK"
     gene            complement(1019..>2190)
                     /locus_tag="PKJ43_06095"
     CDS             complement(1019..>2190)
                     /locus_tag="PKJ43_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ72174.1"
                     /translation="DYLATQMQVIPRYFQLMLLPIGLSIDQGVKIVDGFFNVWSLLGA
                     LFNLAMLGFSIYLVKKEPLISFGILWIYITLFIESSIFPITDPMFDQRMYLPLAGFGL
                     VLLALADRYFFSRNQPLLKPVVVVVLCLLCGATIARNHVFNEPVRVWKDVTQKHPNHL
                     RGWMALGKAYFEQGDFSIPDAIHSFEKAREIAPDHEENLLNLGAAYLATKQNEKAISC
                     FLKLTNSTNPEYKNQALRVMAAYNAGSKNIDESAKYFNDLLVMNPADHKTREALFMLF
                     FNERNFTQAANVAENWLAAYPKNAQAHYFMGKAQFSLNNKSEAEKHLNSALKIDSNHA
                     ESMMLLSNIYVNRFEYDKAISILEKAYAISKNNSIPKNIELIKQLKQKVPAPSQN"
BASE COUNT          704 a          452 c          416 g          618 t
ORIGIN      
        1 tattgtgtcg ccccagatac cacgtatttt caaaaaggta aagcaagaga tttgcaatgg
       61 tgaactttcc acaagttcta tattcttatc acaactgcca cacgaacaac tcattaacga
      121 aatactttcg agccattgtg taatcattcc ttccttgtgc gaaggatttg gttttacggc
      181 tgtagaagct tgttcaatga aaattccgct catatcatcg ggacagggat cacttcccga
      241 ggttgtttcg ggcaacatca ttaccatgga tgagtactca ccacagggcc tcaccgatgc
      301 catgcaaaaa gcaattaacg gaagttttga acatatcgaa gacaaaaaat tcaccgtgga
      361 gacttttgtt gagaaccaca aacaattata ttacaacatt ctgcaaaaca ataaataaaa
      421 aaacaagcat taaagaatca tggcaacatt taaaaacaaa ttcccggtac aggtacgctt
      481 cagcgatgtt gatgttatgg gacatgtttc caatactgtg tatcagaact acttcgacgc
      541 aggcaagctt aattatttcg aagaagtact tcccgaaatg gactttgtta caattggaat
      601 tgtaggggca tcaataaaaa ttgactatct gaaacctatc tttatgaaaa cccgcatttt
      661 agtagaaacc agagtggctc aaatcgggac aaaaagtatt accatggaac attgcattat
      721 cgacgaacat agcggagaac tgctttctac ctgccatgct gttatggttt gtttcgactt
      781 gaaagaacgg cagagcattc ctatccccga ggagtggaaa aagaaaattg cagcctacga
      841 aggtgaccag ttgataaaat gaactcttct aagattaata ccgattgaag aaatataaca
      901 gtccaatctt cgcccactcg attgtactga acatttctat tcatcggcat tatacatgaa
      961 taggttcgga ctgatttaga attgcatctg gtataacatt tgaaaaccgc atcaaacgtt
     1021 agttttgaga aggtgccgga actttctgct tcaactgctt aatcaattca atatttttgg
     1081 ggatagagtt attcttgctt atcgcataag ccttttcgag tatactaatc gctttgtcat
     1141 actcaaaccg gttaacataa atattcgaca aaagcatcat tgattcagca tggtttgagt
     1201 cgattttcaa tgccgaattc aggtgttttt cagcctcgct tttgttgtta agagagaatt
     1261 gagccttccc cataaaataa tgagcctgag catttttagg gtaagctgca agccaattct
     1321 cagccacatt ggccgcttgc gtaaagtttc gttcattaaa aaacagcata aacaaagcct
     1381 cgcgggtttt atggtctgcc ggattcataa ccaacaaatc gttgaaatat ttcgccgatt
     1441 catcaatatt tttacttcct gcattgtagg ctgccatgac tcgtaatgcc tggtttttat
     1501 actctggatt agtcgaattg gtgagtttta agaagcacga aatcgccttt tcgttttgtt
     1561 tagtagcgag gtacgcagct cccaaattca gcaaattctc ttcatgatcc ggagctattt
     1621 cgcgagcttt ttcaaaactg tgaattgcat caggaataga aaaatcacct tgttcgaagt
     1681 acgctttacc taacgccatc catccccgaa gatgattagg atgcttttgg gttacatctt
     1741 tccacacacg aaccggttca ttgaacacat ggtttcgggc aatagtggct ccacaaagca
     1801 aacataaaac cacaacgact accggcttta gcagaggttg atttcggcta aaaaaataac
     1861 gatcggccaa tgccagtaaa accagaccaa aaccagctaa tggcaagtac attcgttggt
     1921 cgaacattgg atcggtaata ggaaaaatcg atgattcgat gaaaagcgta atatatatcc
     1981 acaaaattcc aaacgatatg agtggctctt ttttaaccaa gtatatggaa aaaccaagca
     2041 tcgcaagatt aaacaatgct cccaataaac tccaaacatt aaagaagcca tcaacaattt
     2101 tcaccccttg gtcgatactt agcccaattg gaagcaacat gagttgaaaa tagcggggaa
     2161 ttacctgcat ttgagtagcc agataatcga
//
LOCUS       DAOPUW010000319         2178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_53914_length_2178_cov_2.211493, whole genome shotgun sequence.
ACCESSION   DAOPUW010000319 DAOPUW010000000
VERSION     DAOPUW010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53914_length_2178_cov_2.211493"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PKJ43_06100"
     CDS             complement(<1..358)
                     /locus_tag="PKJ43_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72175.1"
                     /translation="MMKIKNKTIVLLLGLLLAGNHILYGQKSISLEETIGLAQQKSLD
                     AFKFRNLFLADYWNYQSYRSQQKPHLYWYITPMTYNRNITTRYDFENNVEVYRQTQTL
                     NSYSSLSLSQNIVATGG"
     gene            complement(339..>2178)
                     /locus_tag="PKJ43_06105"
     CDS             complement(339..>2178)
                     /locus_tag="PKJ43_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNZ72176.1"
                     /translation="KCEASNMKPQTLKLIAQACISGTNEVIIPMLSSVLVNCAVFLPL
                     IFLSGISGALFYDQAVAIAIGAVSSWAVSITIIPVYYYLIFKKGKGFGENNWLKRING
                     IDYEAVYEKGFRFFIRKQWTVLAMIIIFLFVTVGLFYDLPKSKLPAIEKDELIMSVDW
                     NMPVTIQENRQRVEGFLNEVAPLVIQSTALIGEQQFLLDKDYTATGNESNIYLKSVSS
                     KSVDSIISLANNYFKQRYPEANISYGDADNIFNVIFSDNEPALEARLKATGDYGPYYN
                     KYLKAVNGKINAQLGGNSVPPISWQEHVVLTASPERLLLYGVSYQVLYGKLKSMFNEN
                     QIFTITDNNSFVPVVLGGNPQTLRKIIDESYIQNSSGENIPVRELLDQSVGSDLKSIV
                     AGKEGEYYPLALADSKPKESMQLVKTAVRDDGHFEVSFAGRYFSNKKMVKELFVILLI
                     SLALLYFILASQFESLVLPLIILVEVPLDFFGVFLFLKLGGSGINLMSMIGIVVMAGI
                     VVNDSILKIDTVNQLRKEGYGLLRALLVAGQRRLKPILMTALVTIIGIAPVLFAGGLG
                     ADLQRPLALANIGGMIVGTFVSLYIIPLLYFYLNRKIKVKDDENKK"
BASE COUNT          653 a          500 c          423 g          602 t
ORIGIN      
        1 taccccctgt ggccacaata ttctgagaaa gcgaaagact tgaataagag tttagtgttt
       61 gcgtttgccg gtaaacctct acattgtttt caaaatcgta ccgggtagta atgttccggt
      121 tataggtcat aggcgtaata taccaataaa gatgaggctt ttgctgcgaa cgatacgact
      181 ggtaattcca gtaatcggcc aaaaaaaggt tcctgaactt aaaggcatcg agcgattttt
      241 gctgagccag cccaatggtc tcctccaggg aaatgctctt ttgaccatac aaaatatgat
      301 tcccggctaa taacaatccc aacaggagaa ctattgtttt attttttatt ttcatcatct
      361 tttaccttaa ttttcctatt caaataaaag tatagcagcg gaataatgta caggctcaca
      421 aatgttccca caatcattcc cccgatattt gccagagcta atggccgttg cagatcggca
      481 ccaagtccac cagcaaacaa tacaggagca atgccaataa tcgttaccaa agctgtcata
      541 agtatgggct ttaacctacg ttgccctgcg accaataaag cacgaagcaa cccgtaacct
      601 tctttgcgta attgatttac agtatcgatt ttcaatatcg agtcgttcac cacaatacca
      661 gccattacta caatgcctat catcgacatc aggttgatgc ctgaccctcc cagcttaagg
      721 aaaaggaaaa ccccgaagaa gtcgagagga acttccacca ggatgatgag tggcaacacc
      781 agtgattcaa attgagaggc caggataaaa tagagcaagg ccagcgatat caacaggata
      841 acaaacaact ctttcaccat tttcttgttt gaaaaatagc gaccagcaaa agaaacctca
      901 aaatgcccgt cgtcgcgaac agccgttttt accaattgca tggactcttt gggtttcgaa
      961 tcggccaatg ctaacggata atattccccc tccttgcctg ccacaattga tttcagatcg
     1021 ctgccaaccg attggtcgag caactccctt actgggatat tttctccact actgttctgt
     1081 atgtacgatt cgtcaataat ctttcggagt gtctgtggat tacctccaag taccacaggg
     1141 acaaaactgt tattatcggt aatggtaaaa atctggtttt cattaaacat acttttcaat
     1201 ttaccataca gcacttgata actaacccca tacagcagaa gcctttcggg tgatgctgtt
     1261 aaaaccacat gctcctgcca acttatcggg ggaacactat tcccaccaag ctgcgcattg
     1321 atctttccat tcactgcttt caaatacttg ttgtaatagg gaccataatc acctgtagct
     1381 tttaacctgg cttcgagcgc aggttcattg tcggaaaaaa taacattgaa aatgttatcg
     1441 gcatcgccat acgatatgtt ggcttccgga taacgttgtt tgaaataatt gttggccagc
     1501 gagatgatcg aatctacgga ttttgacgaa accgatttca ggtagatgtt cgattcgttg
     1561 cccgtagctg tataatcctt atccaataag aactgctgtt cgccaataag ggctgtcgat
     1621 tggattacga gaggggcaac ttcgttcaaa aagccttcaa cacgttgcct gttctcctga
     1681 atggtaaccg gcatattcca gtcaacagac ataataagtt catctttttc aatggctggg
     1741 agttttgatt tgggcagatc gtaaaacaga ccaactgtga caaacaaaaa gatgataatc
     1801 attgccaata ctgtccactg ttttctgatg aaaaaacgaa atcccttttc atacaccgct
     1861 tcataatcga tcccgttgat ccgcttcagc caattatttt cgccaaaccc cttccctttt
     1921 ttgaatatca aatagtaata taccgggata atggttattg aaactgccca ggaagatact
     1981 gccccaatgg caatggccac cgcctgatcg tagaacaaag caccagaaat accgcttaga
     2041 aatatcaacg gaagaaaaac ggcacagttt acaagtacag agcttagcat gggaattatc
     2101 acctcattag tgccggagat acaggcttgg gcaattagtt tgagggtttg aggcttcatg
     2161 tttgaagctt cacatttg
//
LOCUS       DAOPUW010000320         2173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_54080_length_2173_cov_2.635033, whole genome shotgun sequence.
ACCESSION   DAOPUW010000320 DAOPUW010000000
VERSION     DAOPUW010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54080_length_2173_cov_2.635033"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="PKJ43_06110"
     CDS             <1..1169
                     /locus_tag="PKJ43_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNZ72177.1"
                     /translation="TGYSTALVAQYFGAGEKQNTTKSAFQAVLIALLAWPLIMLSKPV
                     LSMYFEMARIPASQLGYQNDYLNILIWGSVFSLLRHTLGCYFTGTGKTKIVMTATVLA
                     MIINVILDYILIFGKLGFEPMGVKGAALATISGSFAATLLLFIVYLNKTNQVEFSVLK
                     SFRFNWPIMKKLIVFGSPAGFELFLNFIAFSVMISIFHSKGDVAATATTIMFNWDMVS
                     YIPLLGLEIAVTSLVGRYMGARKPEIAQHAALSAIKIGIFYSLIVLILFVFIPEALVR
                     VFHPAQPSQIFEDAVPIAVNMIKIAALYVLAEAVLVAFVGALRGAGDTFFTMVASVAA
                     HWSFVPVIYLSLYVFNFSVELSWFLLVVFFLMFGVVLGFRFKSGKWKKIKVINN"
     gene            complement(1239..>2173)
                     /gene="cas8a1"
                     /locus_tag="PKJ43_06115"
     CDS             complement(1239..>2173)
                     /gene="cas8a1"
                     /locus_tag="PKJ43_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HNZ72178.1"
                     /translation="MIRFYKSLAGVYNLDKAEENGWTNVIKPDENEVKRLVSEFGIPD
                     DLIQDILDIDERPRMEFDDDWSLLIMRIPVQTPNNGVPFITVPLAVFVTQQQVITICC
                     AENEVLPAEQPSLYRTHYKLVNDHTNFILNLFLRSANTYLKYMKQINQQSNHIEKELE
                     RSIKNEELHNLLNLEKSLVYFITSLKGNDNLLSKFRKSKLSVLNETNEDLLEDAIIET
                     KQAVDTVQIYSDIQSGMMDTFASVISNNINVVMKQLTSFSIILMIPTLIASIYGMNVN
                     NGLETSKLAFLGILIFSIIASTLGVYIFRRKQFF"
BASE COUNT          633 a          395 c          424 g          721 t
ORIGIN      
        1 tgaccggata ttcaactgcc cttgttgctc aatattttgg tgctggtgaa aagcaaaata
       61 ctacaaaatc agcttttcag gctgtactta ttgcgctttt ggcctggcct ctcattatgc
      121 tttcgaaacc agttctatcg atgtactttg agatggccag aataccggca tcacaattag
      181 gatatcaaaa tgattacctg aatatactaa tatggggatc tgttttcagc ctcttacgcc
      241 atacccttgg atgctatttc actggaactg gcaaaacaaa aatagtgatg accgctaccg
      301 tactggcaat gataataaac gtaatattgg attatatact gatatttggg aagttaggct
      361 tcgaacccat gggcgtaaaa ggcgcggcac ttgcaaccat atccggttcg tttgcagcca
      421 cattgctgct ctttattgtg tatttaaata agacaaacca agttgaattt tcggtactca
      481 aatcgttccg tttcaattgg ccgatcatga agaagctcat tgtttttggt tcacctgcag
      541 gatttgaact gtttctgaac tttattgctt tttcagtaat gatttctata tttcacagta
      601 aaggcgatgt ggctgcaact gcgacaacaa tcatgtttaa ctgggatatg gtttcatata
      661 tcccgttgct tggtctcgaa attgccgtta ccagtttggt cgggagatat atgggtgcaa
      721 ggaagcctga aatagcgcaa catgccgcat tatcggctat taaaataggc attttttatt
      781 cgctgattgt tcttatcctg tttgtattca tacccgaagc attggtaaga gtgttccatc
      841 ctgcccaacc atctcaaata ttcgaagatg ctgtgcccat tgcagtaaat atgataaaaa
      901 ttgcggcctt gtacgtgttg gccgaagctg ttttggtagc ttttgttgga gcactgcgtg
      961 gagctggcga tacctttttt actatggtag catcggttgc agcacattgg tcgtttgttc
     1021 cggtaatcta cctttcgctt tatgttttta atttttctgt tgaattaagc tggttcttat
     1081 tggtagtttt ctttttgatg tttggagttg ttcttggatt ccgtttcaaa agtggaaaat
     1141 ggaaaaaaat aaaagtaatt aacaactaag cgtaaactta aaagaggttg caagatataa
     1201 tttgcaacct ctttctttat atgacaatgt taacctgtct aaaaaaactg tttcctccga
     1261 aaaatgtaaa cgccaagagt agaggcaata atagaaaata ttaaaattcc aagaaaggcc
     1321 aattttgaag tttccaaacc gttgttaaca ttcattccat aaatgctggc aataagtgtt
     1381 gggatcatca agataattga aaatgaagta agctgcttca ttacaacatt aatattgttg
     1441 gaaataacag atgcaaaagt atccatcatc ccactttgaa tatcggaata gatttgaacg
     1501 gtatctactg cttgttttgt ttcaattatg gcatcttcca acaaatcttc gtttgtttcg
     1561 tttaatacag aaagttttga ttttctgaat tttgacaaca aattatcatt gccttttaat
     1621 gacgttataa aataaaccaa gcttttttca aggttaagca aattatgcag ttcctcattt
     1681 ttaattgaac gctcaagttc tttttcaatg tgattagatt gctggttaat ttgcttcatg
     1741 tatttcagat aagtattggc tgaacgcaag aaaagattca aaatgaaatt tgtgtggtcg
     1801 ttgaccaatt tataatgagt cctgtaaagc gaaggctgtt cggcaggtag cacttcattt
     1861 tcggcacaac atatagtaat aacttgttgc tgagtaacaa aaacagccag aggtacagtt
     1921 ataaaaggca ctccgttatt gggagtctga accgggatac gcatgatcag tagcgaccaa
     1981 tcgtcgtcaa attccatacg aggccgctcg tctatatcga gaatatcctg aataagatca
     2041 tcgggaatac caaactcaga aacgagtctt ttaacttcat tttcatcggg cttaatgaca
     2101 ttagtccacc cattttcctc tgctttgtcg aggttataga caccagcaag gcttttgtaa
     2161 aatctgatca ttt
//
LOCUS       DAOPUW010000321         2171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_54162_length_2171_cov_2.415879, whole genome shotgun sequence.
ACCESSION   DAOPUW010000321 DAOPUW010000000
VERSION     DAOPUW010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54162_length_2171_cov_2.415879"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..>2171
                     /locus_tag="PKJ43_06120"
     CDS             18..>2171
                     /locus_tag="PKJ43_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72179.1"
                     /translation="MKQGAVIHVFRLTIALLFAAMLFISCQFTEQQLITPIEAVPSTS
                     PVFVKINDMSSFPQLFSKQNKWWAELGKLEAIKNLDNSILSLDSAYNSNQELKLFCQK
                     KEIIVSFSSSATQNIETMAIIPIALSGEQKKANNFFNSLSKTKNINVSKRKFGRNKIY
                     EAKSAEGNLLFLYTFSHKLLLISKTPRLIEEALTQIEKSDREADRSLMPLLKTTNNQA
                     QANLFVNHQLASEIFSPVLSEEMAIQTEKLSSYAAWTELDITIKEDKILLSGFTNGNA
                     QSNFFVTLLQGQEPGISKIESVLPSNTTFFSGYYLSDISAFFSGLSKYKSQDVNAAPV
                     ISPMQQLFIEIFDGEMARATFAPDISPSKLGTLFAIKTKSGSYALGKLNQHFEKQGLY
                     EKEFVVDNQTKYIFYRSPIVNLTNILFGNMFAETPTVWYTSYGNYLLFSDSFSGLGEV
                     IMSNILGETLLADVSYNKFQSSLSSKNNYYFYCNTSNFFDHAQLFFNEKISSEIKAQD
                     DFRKFTHFAWQTSSSGDMAYNNSSIVYDENIRLKPQTVWQSRLAAPLAKRPYIIEDKN
                     DTQNRLLVLVDTLNNLYLMNNVGRIVWQMNTGAQILGEFSLVELGKNKDTHLVFNTKE
                     KIFMIDKDGKSAKNFPVLFKMTATNGVSVFDYENNRNYRFFFACEDQRVYAYDIEGKP
                     VEGWNPPKTDHPINKEVQHIVVEGKDYIIVSDLMKD"
BASE COUNT          737 a          455 c          392 g          587 t
ORIGIN      
        1 caaaaagaat gaaaaaaatg aaacagggtg cagtgattca tgttttccgg ctaactatag
       61 cccttctctt tgcagcaatg cttttcatta gttgtcaatt tacagaacag caactgataa
      121 cacctattga ggctgttcct tctacgtcgc ctgtgttcgt aaaaattaac gatatgagct
      181 cgttcccaca acttttttca aaacagaaca aatggtgggc tgaattgggc aaacttgagg
      241 caataaaaaa tcttgacaac agtattttat ccttagactc tgcatacaat tccaatcaag
      301 aactgaaatt gttctgccaa aagaaagaaa ttattgtatc attctcttcc tccgcaacac
      361 aaaatattga aacgatggct attattccaa tagcactgag cggggaacaa aagaaagcca
      421 acaatttttt caacagcctt agtaaaacga agaatattaa tgtctctaaa agaaaatttg
      481 gcaggaataa aatatacgaa gcaaaatcgg cagaaggaaa cttgctgttt ttgtacactt
      541 tcagtcacaa attactgcta atttcaaaaa cgccccgttt aatcgaagag gcactcactc
      601 aaatcgaaaa atctgaccgg gaagccgaca ggtcattaat gcctttacta aaaactacaa
      661 acaatcaggc tcaagcaaat ctttttgtaa atcaccaact tgccagcgag attttctctc
      721 ctgttttatc agaagaaatg gctatccaga ctgaaaaatt gagcagttat gccgcttgga
      781 ccgaactcga tataacgata aaagaagaca aaattttgtt gagcggtttt acgaacggca
      841 acgcacaaag caactttttt gtcacattac tacaggggca agaacctgga atctcgaaaa
      901 ttgaaagtgt tttgccgtcg aatacaactt tcttcagtgg ctactacctt tccgacatat
      961 cggctttttt ctcaggacta agcaaataca aaagtcaaga tgtgaatgcc gcacctgtta
     1021 tttctccaat gcaacaactt tttatcgaga tattcgatgg tgaaatggca cgggcaactt
     1081 ttgcccccga tatttcgcca tcaaaacttg gcaccctctt cgccataaaa accaaaagtg
     1141 gaagctacgc actaggcaaa ctaaatcaac actttgagaa gcaaggactt tatgaaaaag
     1201 agtttgtggt cgataaccaa acaaaataca ttttttaccg gtctccaatt gtgaacctta
     1261 ccaatattct tttcggaaac atgtttgccg aaacgccaac cgtttggtac accagttatg
     1321 gaaattacct tttattctcc gattcatttt cgggccttgg ggaagtaatc atgtcgaata
     1381 ttcttggtga aactctctta gccgatgtca gttacaataa attccagtcg agcctctcgt
     1441 caaagaacaa ttactatttc tattgcaaca catcgaattt ctttgaccat gcccagctct
     1501 ttttcaatga aaaaatctct tccgaaataa aggctcaaga cgatttcaga aagttcaccc
     1561 attttgcctg gcagacaagc tcttcgggcg atatggccta taacaattcc agtattgttt
     1621 acgatgaaaa cataaggctt aagccacaaa cagtatggca aagccgccta gcagcgccac
     1681 ttgcaaaacg gccatacatt attgaagaca agaatgatac tcaaaacagg ttgttggttc
     1741 tcgttgatac actcaacaat ctgtatctaa tgaacaacgt tggacggatt gtttggcaaa
     1801 tgaacaccgg agcacaaatt ctaggtgaat tcagccttgt tgaactcgga aaaaataagg
     1861 atacacatct ggtattcaac acaaaagaaa agatattcat gatcgacaaa gatgggaaat
     1921 cagccaaaaa tttcccggta ttgttcaaaa tgacagccac gaacggagtt tcagtgttcg
     1981 attacgaaaa caatagaaac tatcgtttct tttttgcttg cgaagaccaa cgggtttacg
     2041 catacgacat tgaaggaaag ccagttgaag gatggaatcc tccaaaaacc gatcacccca
     2101 tcaacaaaga agttcaacac atagttgttg aagggaaaga ttacattatt gtgtctgacc
     2161 tgatgaaaga t
//
LOCUS       DAOPUW010000322         2170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_54194_length_2170_cov_2.663830, whole genome shotgun sequence.
ACCESSION   DAOPUW010000322 DAOPUW010000000
VERSION     DAOPUW010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54194_length_2170_cov_2.663830"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="PKJ43_06125"
     CDS             complement(<1..1269)
                     /locus_tag="PKJ43_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNZ72180.1"
                     /translation="MEVKTLGGLIKRSAEQFSDKPALGYVGEKPVTYGEVILKINSIV
                     AFLEKQGITSDDKVAILSSNQPNWGITYFSLATMGATIVPILPDFTEQEIANVLNHAE
                     VKAIFVSEGLYRKVLDFKSEHLKLIVSIENFALVPEGTVEKELPTLESSVVDTDKPVN
                     QYEIDPDRVASIIYTSGTTGKSKGVMLTHKNLVFSARQSGVIHPMNTTDSMLSVLPLS
                     HTYENTIGLILPLMYGTAIYYLRKPPVPSVLIPAMKQVRPTIMLTVPLIMEKVYKGKV
                     LPEINGKAITRTLYKFTPTRKLLNKVAGKKLYETFGGRLKFFGIGGAKVDPQVERFLI
                     EANFPYAIGYGLTETSPLLAGFDSYKGRYQSTGPTMEGVTLKINDPDPITGEGEIWAK
                     GDNVMKGYYKEPEITSEVLTPDGWFRTGDLG"
     gene            1529..>2170
                     /locus_tag="PKJ43_06130"
     CDS             1529..>2170
                     /locus_tag="PKJ43_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ72181.1"
                     /translation="MENNEIIKCIAETVKQQQMNESGGHDWWHTFRVWKMARHIAEQE
                     NGNQFIVQISALLHDIADWKYNEGNEFAGGEKARELLCGFSLSKATITEICSIVDNIS
                     FKGAKTQVSELSHNGKIVQDADRLDAIGAIGIARTFAYGGLRGRPMYAPEIRPQRHKS
                     FDEYKKSVTPTINHFYEKLLLLKDRMNTPTAKQIAEERHRWMELFLEQFYREWY"
BASE COUNT          696 a          445 c          423 g          606 t
ORIGIN      
        1 cccaagatca cctgtacgaa accaaccatc gggagttaac acttcactgg ttatttcagg
       61 ttctttgtaa taccctttca ttacattatc accttttgcc catatttcgc cttcgccagt
      121 aattggatct ggatcgttaa ttttaagagt aaccccttcc atagtaggac cagtagactg
      181 ataacgtcct ttgtatgaat cgaaaccagc taacaacggc gatgtttcag tgaggccata
      241 gccgatggca tacgggaaat tggcttctat aagaaatcgc tctacctgag gatcaacctt
      301 ggctccacca atgccgaaaa atttaagcct gccgccaaat gtttcatata attttttacc
      361 agcaacctta ttaagtagct tacgtgtagg tgtaaattta tacagggtac gagtaatggc
      421 tttcccattg atttcgggca atacctttcc tttatatacc ttttccataa tcagcggaac
      481 ggtaagcata atggttggac gaacctgttt catagcagga ataaggactg atggcacagg
      541 tggcttacga agatagtaaa ttgcagtgcc atacataagt ggaagaatca gaccgatcgt
      601 attttcatag gtatgagata gcggaagaac cgacaacatg ctgtctgtag tattcatcgg
      661 atgaattact cccgattgcc ttgccgagaa aacaaggttt ttatgggtaa gcattactcc
      721 ttttgatttt ccagtggtgc ctgatgtata aatgatagac gccacgcggt cgggatcaat
      781 ttcgtattgg ttgaccggtt tgtctgtgtc aactaccgat gactccaatg tcgggagttc
      841 cttttcgaca gtaccttcgg gtactagcgc aaaattttca attgatacaa taagtttcag
      901 atgctcgctt ttaaaatcta aaaccttccg gtacaaacct tctgaaacga aaatagcttt
      961 tacctctgca tggttaagta cattggcaat ttcctgctca gtaaaatcgg gcagaattgg
     1021 cacaattgta gctcccatgg tggccagaga aaaatacgta ataccccagt tgggctggtt
     1081 agagcttagt attgctactt tgtcatcgct tgtgattccc tgtttttcaa gaaacgcgac
     1141 gattgaattg atttttagga taacttcacc ataagtgacc ggcttttctc ccacatatcc
     1201 taatgccggt ttgtcgctga attgttcggc agatcgctta atcagaccgc ctaatgtttt
     1261 tacctccatt aacccattta taaaataata caacaaagga actaaatata ttcccgaatt
     1321 gttctcaaac caataatttt acgcaataaa attagcacaa gactctgaat gtcaaaacat
     1381 aaaactcaaa aaatgtttga aaaaaaaatc caatagttca aaaatatgag aagtaatagt
     1441 attttgtcgg acatttcaat atctgaaagc aaacaataac caaagtaacc aataaaattt
     1501 ttagaaatat attgcctaaa aagagctaat ggagaataac gaaataataa aatgcattgc
     1561 tgaaacagtt aagcaacaac aaatgaacga aagcggtggt cacgactggt ggcatacatt
     1621 ccgcgtttgg aaaatggcca gacacattgc agaacaagaa aatgggaacc aatttattgt
     1681 gcaaatatcg gcattacttc acgacatagc agattggaaa tataacgaag gaaacgaatt
     1741 tgccggagga gaaaaagccc gtgaactact ttgtggcttt tcgttgtcaa aagcaactat
     1801 aacagagata tgctcaattg tcgataacat ttcattcaaa ggagccaaaa cccaggtaag
     1861 tgagttaagc cataacggga aaattgttca agatgcagac cgcctcgatg ccattggagc
     1921 cattggtatt gcacgtacat ttgcctacgg ggggttgaga ggccggccta tgtacgcccc
     1981 cgaaatacga ccccagcggc ataaatcttt cgatgaatat aaaaaatctg ttaccccaac
     2041 aatcaaccat ttctacgaaa aattactttt actgaaagat cgaatgaata ctcctacagc
     2101 taagcaaatt gccgaagaac gccatcgttg gatggaactt tttcttgaac aattctatcg
     2161 cgaatggtac
//
LOCUS       DAOPUW010000323         2170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_54196_length_2170_cov_2.619858, whole genome shotgun sequence.
ACCESSION   DAOPUW010000323 DAOPUW010000000
VERSION     DAOPUW010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54196_length_2170_cov_2.619858"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PKJ43_06135"
     CDS             complement(<1..625)
                     /locus_tag="PKJ43_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72182.1"
                     /translation="MKKIVYTLLLMLVAPFWLMSQNAIQEIWTQPAAFKADEMISVFF
                     DVTGTPLDGIPESEGVRVWTWFPNNPDENWANPSEKTRLKHVNGNIWRWDLNPAELYN
                     LPANQITGIYGQLQNYAGTKITDAFAPDKGNAITVYSLSTILADDAIIEYYPKQFTQD
                     RPLSILINANNTWSDCESTPAKGKLASAPEDRMHGGVNGWEIQVMNNT"
     gene            complement(643..1158)
                     /locus_tag="PKJ43_06140"
     CDS             complement(643..1158)
                     /locus_tag="PKJ43_06140"
                     /inference="COORDINATES: protein motif:HMM:NF027710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5004 domain-containing protein"
                     /protein_id="HNZ72183.1"
                     /translation="MKKTSLILMFILVMQLIGCNKTDDGVFTEPITMYEKMVGTWTIS
                     KVAQKDLIAIAASSKPDQILLTNKFDFKTFTITLNVDEKFAPTTFEVGGTSPQLFLQS
                     GYWKLNNPFPNTDGKPLVIELYSDEAKTVLIDELSISTVPGAKKTLVFDLIRESNGVA
                     YVDYEYSLKAN"
     gene            complement(1494..>2170)
                     /locus_tag="PKJ43_06145"
     CDS             complement(1494..>2170)
                     /locus_tag="PKJ43_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72184.1"
                     /translation="KPVEGWNPPKTDHPINKAVQHIVVEGKDYIIVSDLMKDYIFDRK
                     GNVRVPTNFVYRHSFLNEVYLEKRTKNNEPRLVTTDSEGILHYTYLNGKHEKKSVKNL
                     DDNHFFVTANINADVEPEYIFTQGNQLFVTNINGDSLFCIPFSGQSLLKPFIYTFSEK
                     EKKIGVTDPQSNKIYLFNSNGNLYKGFPLDGNTNFNIGMSKGQPTRFNLYVGSPDGNL
                     YNYLIE"
BASE COUNT          567 a          428 c          448 g          727 t
ORIGIN      
        1 cagtattgtt catcacctga atttcccagc cgtttactcc accatgcatc ctatcctcag
       61 gtgcgctggc cagtttccct tttgccggag tcgattcgca atcgctccaa gtattgttgg
      121 cattgattaa aatagaaagc ggcctgtctt gcgtaaattg cttaggataa tattctatta
      181 tagcatcgtc ggcaagtatg gtgcttaagc tgtacaccgt tatggcgttc cctttgtcgg
      241 gagcaaaagc atcggttatt tttgtgccag cgtagttttg taattgtcca tatattccgg
      301 ttatttggtt ggccggaagg ttatataatt cagcggggtt gaggtcccag cgccagatgt
      361 tcccgtttac gtgcttcagc cttgttttct ccgaagggtt tgcccagttt tcgtcaggat
      421 tgttgggaaa ccatgtccat acccgaactc cttctgactc aggaatacca tcgaggggag
      481 tgccagttac gtcaaaaaag accgagatca tctcatctgc cttaaatgct gcgggctgtg
      541 tccaaatctc ctgaatagcg ttttgcgaca tcagccaaaa tggagcaacc aacataagca
      601 gtaatgtata tactattttt ttcattgtta atgaagtgtt tgttaatttg cttttagtga
      661 gtattcataa tcaacatagg caacgccatt cgattcgcgt attagatcga ataccagggt
      721 cttttttgct cctggtacag tacttatact aagctcgtct attaataccg tttttgcttc
      781 atctgagtaa agttcaatta ccagaggttt tccatcggta tttgggaaag ggttgtttag
      841 cttccaatac cctgattgca aaaaaagttg aggcgatgtt cctccaactt cgaatgttgt
      901 aggagcaaat ttctcatcta catttaatgt gatggtgaac gttttgaaat cgaacttgtt
      961 ggttagcaat atctggtcgg gtttcgacga tgcggctatg gcaataaggt ctttttgggc
     1021 taccttcgaa attgtccatg ttccgaccat tttttcatac atagtaatgg gctcggtgaa
     1081 taccccgtca tcggttttat tgcagccaat gagttgcatt accagtatga acatcaaaat
     1141 taagcttgtt ttcttcatag attatttgtt tagtttctaa ctcatgattt gttaaagtgc
     1201 cttttggctc tttgctaaac aaatgtatcc cgatcggtac ccaacaaaaa tcttttcagg
     1261 tgcaatatag acttttatga acgcagttgt cgggtaatgt gcagtagtat aagacgttga
     1321 ttgcgttgtg tgccgacaaa aatatagatg gaatatgaac ggttttatga aatatccacc
     1381 ccaaattcat tccttttagg tataaaacca tttaatagca aaggatagtt gtagtatttc
     1441 cgttaacagg caacgttgat tccccggtta cgaacgttca tcgaagacgc aacctattcg
     1501 attaaatagt tgtataaatt cccgtcggga ctgcctacat aaaggttgaa acgggtgggt
     1561 tgcccttttg acattccaat gttgaaattg gtattcccat cgagtggaaa gcctttgtac
     1621 aaatttccgt tgctgttaaa caagtatatt ttgtttgatt gaggatctgt aaccccaatc
     1681 tttttttctt tttctgaaaa agtataaatg aacggtttca aaagagattg cccgctgaag
     1741 gggatacaaa acaagctgtc cccattaata tttgtcacaa aaagttggtt cccttgtgta
     1801 aaaatatact caggttcaac atcagcatta atatttgcag ttacaaagaa atgattgtca
     1861 tccagatttt tgacgctttt cttctcatgc ttcccgttga ggtaggtgta gtgtaaaatg
     1921 ccttctgagt cggtggtcac aagcctcggt tcattatttt tagtcctttt ctcaaggtaa
     1981 acttcattta agaaagaatg cctgtataca aaatttgtag gtacccgcac gttacctttc
     2041 cggtcgaaaa tgtaatcttt catcaggtca gacacaataa tgtaatcttt cccttcaacg
     2101 actatgtgct gaactgcttt gttgatgggg tgatcggttt ttggaggatt ccatccttca
     2161 actggctttc
//
LOCUS       DAOPUW010000324         2168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_54287_length_2168_cov_2.197823, whole genome shotgun sequence.
ACCESSION   DAOPUW010000324 DAOPUW010000000
VERSION     DAOPUW010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54287_length_2168_cov_2.197823"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..1303
                     /locus_tag="PKJ43_06150"
     CDS             188..1303
                     /locus_tag="PKJ43_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNZ72185.1"
                     /translation="MSETVIFVVLVLGIVSVLMLIAKIIFKKSILFKVGSIILTLIEI
                     VAILAFLIAKLGFGQLYWALPIGLSIVSLMFYFLHTTLRVPTKLLKEDIVNQLATGRL
                     NFSFNEKVTKLNDEFGEMAQSLEIVRKQMFLSISEIKTVSHEISTSATQQTQAALQIS
                     NGASEQASTTEEISSTFEEISASNEQNSSNANTTAAISKDTQAAMNEVNKVVGETLAA
                     IQTITERINIINDIAFQTNILSLNASVEAARAGEHGRGFAVVAGEVRSLAESSKKAAS
                     EIQLLSSQTKTITQKAEQMVGQLTADIVKMTELVTEISIASNEQSISTNQVNSAVFEL
                     NNLAQQNATLSEEMAANAEGLSSQSLKLNESVGFFKL"
     gene            complement(1401..>2168)
                     /locus_tag="PKJ43_06155"
     CDS             complement(1401..>2168)
                     /locus_tag="PKJ43_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ72186.1"
                     /translation="SGAGKTTLLRILSGLLIPDKGVVKFGDTVWYDSEKKINLPPQQR
                     NIGFMFQDYALFPNMTVEENILFAQTVKDVKAASELIYIFGLNELRKRKPAKLSGGQK
                     QRVALARALARKPQLLLLDEPLSALDAEMRVALQDEIRRAHELLGATTLMVSHDLNEV
                     FRLASQVIMIENGAIKKTGKPETVFSDSTISGKVQITGQIARIERQEPIYIVTVISGS
                     NQIIKVIAFDSDLENLKIGDSVMVYTKAFNPIISRLG"
BASE COUNT          647 a          477 c          468 g          576 t
ORIGIN      
        1 ttttttcaac acttcttgct tctaaaaaaa gtaaaatcaa gaatgcaaca cgtttgtaaa
       61 tctgcactgt acaattcaaa acttcttaac cttatagaaa catacccata aaagcaccct
      121 gtaaaaacgg gcgaagcatt ctttttccta tttttgcagc ctgaaaaaat aagggggaga
      181 gggtattatg agcgagacag taattttcgt tgttttggtg ctgggcatcg tgtcggtgct
      241 aatgttaata gctaaaataa ttttcaaaaa atctattctt tttaaagttg gatcaataat
      301 actaacctta atcgaaattg ttgccattct ggcatttttg attgccaaac ttggttttgg
      361 acagttgtat tgggctttac ctattggatt gtcaattgtt tcgttaatgt tttacttttt
      421 gcacaccaca ttgagggtgc caacaaaatt gctcaaagaa gacattgtga accaacttgc
      481 taccgggcga ctcaatttca gcttcaacga aaaggtaacc aaactaaacg acgagtttgg
      541 cgagatggca caatcgcttg aaatagtgcg caaacagatg tttttgtcga tttcggaaat
      601 aaaaacagta tcgcacgaaa tttcgacatc ggctacccag caaactcaag cagcccttca
      661 aatatcaaat ggggcaagcg aacaggcatc gaccaccgaa gaaatttcat caacttttga
      721 agaaatttcg gccagtaacg agcaaaacag cagcaatgcc aacaccacag ctgctatttc
      781 aaaagataca caggctgcta tgaacgaggt aaacaaagtg gttggcgaaa cattggccgc
      841 aattcaaacc atcaccgaac gaatcaacat tattaacgac atagctttcc aaaccaacat
      901 actttcgtta aacgcatcgg ttgaagcagc ccgtgcaggt gaacacggac gagggtttgc
      961 ggtagttgcc ggcgaagtga ggtcactggc agaaagcagc aaaaaagccg ccagcgaaat
     1021 ccagctatta tcatcacaaa ccaaaaccat tacccagaaa gccgagcaaa tggttgggca
     1081 acttacagcc gacattgtga aaatgaccga gttggttaca gagataagca tagccagcaa
     1141 cgaacaaagt atcagcacca atcaggtaaa cagtgctgtt tttgaactaa acaatttagc
     1201 ccaacaaaac gcaacccttt cggaagagat ggccgcaaat gccgaagggc tttcgtcgca
     1261 atcgctgaag ctcaacgagt ctgttgggtt cttcaaactg tagaatatta taattgtttt
     1321 tcaacgggtt aattcattcc gaagatttaa gccactcaat tttatttcgt ttagaacgat
     1381 gtaaaaccat ttgaaagact ctatcccaac ctactaataa tcggattgaa cgccttggtg
     1441 taaaccatta cactatcacc aatttttagg ttttcgaggt cgctgtcgaa ggcaatgact
     1501 ttgatgattt ggttgctgcc cgaaatgacg gttacaatgt agatgggctc ttgccgctcg
     1561 atgcgggcaa tttgccccgt gatttgcact ttgccgctga tggtgctgtc ggaaaaaacc
     1621 gtctctggtt tgccggtctt ttttatcgcc ccgttttcga tcatgatcac ttgtgatgcc
     1681 aaacggaaca cttcgttcaa atcgtggctc accataaggg tggtagctcc aaggagttcg
     1741 tgtgcccggc ggatttcatc ttgaagcgca acacgcattt cggcatcgag ggccgagagg
     1801 ggttcatcga gcaagagcag ttggggttta cgagccaagg cacgggctag ggctacacgc
     1861 tgtttttgtc cgcccgaaag cttggcaggt ttgcgtttcc gcaattcatt caacccaaaa
     1921 atgtagatca attccgaagc ggctttcaca tctttcactg tttgggcaaa caggatattc
     1981 tcttccacag tcatattggg gaaaagtgca taatcctgaa acataaagcc aatattgcgc
     2041 tgctgtggag ggaggttgat tttcttttca gagtcgtacc aaaccgtatc gccaaacttt
     2101 actacaccct tgtcagggat gagcaaacca gagagaatcc tgagcagagt tgtctttcct
     2161 gcccccga
//
LOCUS       DAOPUW010000325         2167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_54318_length_2167_cov_2.785038, whole genome shotgun sequence.
ACCESSION   DAOPUW010000325 DAOPUW010000000
VERSION     DAOPUW010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54318_length_2167_cov_2.785038"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PKJ43_06160"
     CDS             <1..748
                     /locus_tag="PKJ43_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ72187.1"
                     /translation="PSGAGKSTTQKVMTGILKNYQGSVTLMGREVNNFNKQFYENIGV
                     AFEFPNLYLKFTALENLALFASFYTCEIYDFHELLSRVGLWEDRNLPVEAFSKGMRMR
                     LNFIRAILHKPKLLFLDEPTSGLDPSNSRMLKDFIFELRDNGTTIFLTTHNMTDADEL
                     CDRVAFMIDGQLPVIDSPEKLKLQHGRKTLKIRYKNDMQIETKEFSLHGLGSNNEFLQ
                     LINHNEIETIHTQEATLEDIFLAVTGKQLT"
     gene            821..1519
                     /locus_tag="PKJ43_06165"
     CDS             821..1519
                     /locus_tag="PKJ43_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72188.1"
                     /translation="MIKNTVQLLKLTRFDILFQFKQGFYLVYAIITVVYLLLLFYLPE
                     TVRPEVTAYLILSDTSVLGMVFVGALVLLEKQQNVLQSLFITPMKLSTYIFSKAISLT
                     LIATLVSSAIGFIPGAMLDRCLSMLASVILCSLFFTFLGLGVSSRANTLNEYFGGILL
                     AGLIMVVPLALFFIHPGLSLIFPINAAITLLITPESQQTLVSNIAGVSVLVCWNIVAY
                     RYAMNQFNKNIIHK"
     gene            1527..>2167
                     /locus_tag="PKJ43_06170"
     CDS             1527..>2167
                     /locus_tag="PKJ43_06170"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ72189.1"
                     /translation="MTRDLSLIKGDLKIIGRDPMLILSFVAPFLLLIIALFLFPIASQ
                     LSIKYFSLLLDSYFLLGSFFFFSLIPMLFGMIYGFILLDERDEGIISYLSITPLGKTG
                     YLRTRMIIPVLASFVFGVAFLILTGFSSLLNWFEIIVITLIVATEAPLMLLFLAAFAG
                     NKVEGIALSKSFGIVLLPVLIDYFTQGNWRWLLSFSPLWWIERAIFSLQEYRWL"
BASE COUNT          569 a          432 c          448 g          718 t
ORIGIN      
        1 acccagtggg gcagggaaga gcactacgca gaaagtgatg acaggaatac taaagaatta
       61 tcagggcagt gttaccctta tggggcggga agtgaacaac ttcaacaagc aattctatga
      121 aaacattgga gtagcttttg agtttccaaa cctatacctc aaattcacag cgctcgaaaa
      181 ccttgctctt ttcgcctcgt tctacacttg tgagatatat gattttcatg aattgctcag
      241 tcgggtgggg ttgtgggaag accggaacct gcctgttgag gcattttcga aagggatgcg
      301 tatgcgcttg aattttatcc gtgcaatttt acacaagccc aaattattgt ttctcgatga
      361 gccaacttcg ggcctcgatc caagcaacag ccgcatgctg aaggatttta tttttgaact
      421 ccgggataat ggaacgacaa tatttctaac tacacataac atgaccgatg ccgatgaatt
      481 atgcgaccgc gtggcattta tgatcgacgg gcagctccct gtaatcgact ctcctgaaaa
      541 gctaaaactt cagcatggcc gaaaaacctt aaagattcgt tacaaaaacg atatgcagat
      601 agaaaccaag gagttttcat tgcatggtct tggctccaac aacgagttcc tgcaattaat
      661 taatcataac gaaatagaaa cgatccatac tcaggaagcc acacttgaag atatttttct
      721 ggccgttaca ggcaaacagc ttacatagag ataccttgtt tggagtgata tgtgtgtttc
      781 ttatcctcag ataatcaaga atataaacag agaaaataac atgataaaaa atacagtaca
      841 actattgaaa cttactcgtt tcgatattct atttcagttt aagcaaggtt tttatcttgt
      901 atatgccatt ataactgttg tttacctgct gctgttgttc taccttccgg aaacagttcg
      961 ccccgaggtt acggcttatc ttatcctgtc agatacttca gtgctgggga tggtctttgt
     1021 tggagcgttg gttttgctcg aaaagcaaca aaatgtactc cagagtcttt ttataactcc
     1081 catgaaacta tctacataca tttttagtaa agccatctcg ctaaccctaa ttgccaccct
     1141 tgttagttcg gctattggat ttattccggg agcaatgctc gataggtgcc tctctatgct
     1201 ggcatctgtg attctttgtt ccttgttttt tacgttttta ggattgggtg tttcttcgag
     1261 agccaatacg ctgaatgaat attttggagg catacttttg gccggactga ttatggttgt
     1321 cccattagcc ctgtttttta tccatccggg gctgtcgtta atatttccaa tcaatgccgc
     1381 catcacattg ctgattacac ctgagtctca acaaactctg gtaagtaata ttgccggtgt
     1441 ttctgttctt gtttgctgga atattgtggc ttacagatat gcgatgaatc aattcaataa
     1501 aaatataatc cacaaatagt taaaagatga cacgagattt atctttgata aaaggcgatt
     1561 tgaagattat tgggcgtgac ccaatgctga ttttgagttt cgttgcgcca tttctgttgt
     1621 tgataattgc actattctta tttcctatag cctcacaact ctccatcaag tacttttctt
     1681 tgctcctcga ttcctatttc ctgttgggaa gctttttttt cttttcgttg attcccatgt
     1741 tgtttggtat gatttacggg tttatccttc tcgacgaacg cgacgaaggg atcattagtt
     1801 atttatcgat aactccattg ggcaaaacag gatatttgcg gaccagaatg attattccgg
     1861 tgttggcaag ctttgttttt ggagtggcgt ttcttattct gacaggcttt tcatctctgc
     1921 ttaactggtt cgaaataatt gtaattacat tgattgttgc aactgaagcg ccactaatgt
     1981 tgcttttctt ggccgcattt gccggaaaca aggttgaagg gatagcttta tctaaaagtt
     2041 ttggtatagt tctgcttcct gttttaatcg attattttac gcagggcaat tggcgttggc
     2101 tgctttcatt ttcgcctctt tggtggatcg aaagagccat attctcactt caggaatatc
     2161 gatggct
//
LOCUS       DAOPUW010000326         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_54396_length_2165_cov_2.727962, whole genome shotgun sequence.
ACCESSION   DAOPUW010000326 DAOPUW010000000
VERSION     DAOPUW010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54396_length_2165_cov_2.727962"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PKJ43_06175"
     CDS             <1..644
                     /locus_tag="PKJ43_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNZ72190.1"
                     /translation="VTDSNFDVSSEVTFNYTPDRDELNQRIYTQNATIHAFEKQLAIE
                     KLQLKENYRTLLPQVNLSGGYYFQHITNNNLDADNRSQSRSLWPQIGASMVIPIFRAG
                     NLQRQVALSKIEVDIAQYNLENTKNQVNTELLNALNTFESQQQLLTIEKENNELAREN
                     LDISLQRMRLGEATSLEVRMAQNDFEESATRLTNYRYALKIAETRLKQLLGEL"
     gene            complement(1026..>2165)
                     /gene="gshAB"
                     /locus_tag="PKJ43_06180"
     CDS             complement(1026..>2165)
                     /gene="gshAB"
                     /locus_tag="PKJ43_06180"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF002688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HNZ72191.1"
                     /translation="DPAKRDVHQIIAGTEAKGYIAFHLEKARQYLADSQKNSFSFRGF
                     EDLELSTQLVLREAVRRGVAFEILDRDENFVRLSRDGNVQYIQQATKTSLDNYAGILA
                     MENKVVTKKILASQGIRVPKGMSYSDVQTALSDFSLFSGQAIVIKPKTTNFGKGITIL
                     KENTNAAVYERAVAIAFEEDKNILIEEFIAGREFRIFVMDNEVVGILHRVPANVTGNG
                     KLTIGELVAMKNEDPLRGKGYRTPLEKINLGEAEEMFLALQGKTFKTIPADGETVFLR
                     ENSNISTGGDSIDFTDDVHESYKAIAVKAAQALNVRITGLDMIITNYAQEANAENYAI
                     IEMNFNPAIHIHCHPYKGKNRRLNEKLLDMLGFAAPVALGSQHNL"
BASE COUNT          578 a          467 c          450 g          670 t
ORIGIN      
        1 tggttaccga tagcaatttt gatgtttctt ctgaagttac cttcaattac acacccgatc
       61 gcgatgaatt gaaccaacgc atatatacac aaaacgcaac gatacacgct tttgaaaagc
      121 aattagctat tgaaaaattg cagttgaaag agaattatcg cacgttgttg ccacaggtta
      181 atcttagcgg aggatattat tttcagcata ttaccaacaa taatcttgat gcagacaacc
      241 gttcgcaaag ccggtcgttg tggcctcaaa ttggggcttc gatggtgatt cccatatttc
      301 gtgcaggtaa tttgcagcgg caggtagcgt tatcgaaaat tgaggtggat attgctcaat
      361 acaatctcga aaacacaaaa aatcaggtta atacagaatt gcttaatgca ctaaatactt
      421 ttgaaagcca gcagcagctt ttaacgatag agaaggaaaa caatgagttg gcccgcgaaa
      481 atctcgatat aagtttgcaa cggatgcgtt tgggcgaagc tacttctctt gaagtacgaa
      541 tggcgcaaaa tgattttgaa gagtcggcaa cccggttaac caattacagg tatgcgttga
      601 aaatagcaga aacccgtctg aaacagttac ttggcgagtt ataagagcta caacctgcgt
      661 accatttctt tatggctgtt tttacaatgg attgaatttg agtatttttc agcactcaaa
      721 tatgaatttt aagtctaagt tactctttta ataatgttcg ttatggctca gggagcggta
      781 taccaattgt gaagttatcc actccctgtg tctacgagcc tattcattct caaccatttc
      841 gtaagttatt tcactgataa aggtttcaat gatttcatca cgcaatggca acttctctgt
      901 aaacaagtag tttttcatta ggtctaatat tccgaaagaa caaggggtta tattaaaatc
      961 atcttcttat tttctgcctt tcttttgaat gaatctactt tctgaatcaa ggcgttgcat
     1021 tgagttcaca aattgtgttg cgaacccaat gcaacaggtg cagcaaagcc cagcatatcg
     1081 agcagttttt cgttcagcct gcggtttttc cctttgtatg gatgacaatg aatgtggatt
     1141 gcagggttga aattcatctc aattatggca tagttttcgg cattggcttc ctgggcataa
     1201 ttggttatga tcatatcgag ccccgtgatt ctcacattca gagcttgtgc agcttttacg
     1261 gcaattgctt tgtacgattc atgaacatca tcggtaaagt cgatgctgtc accaccggtg
     1321 ctgatgtttg aattctcgcg cagaaagacg gtttcaccat cggccggaat agttttgaat
     1381 gtttttccct gcaaagcgag gaacatttct tcggcctcgc ccaggttaat tttttcgagc
     1441 ggggtccgat aacctttccc ccgcaaggga tcttcatttt tcatggccac cagctccccg
     1501 attgtcagtt tgccgtttcc ggttacgttg gccggaacgc ggtgcagtat tcccaccact
     1561 tcattgtcca ttacaaaaat gcggaactcc ctgcctgcaa taaattcttc gatcagaata
     1621 tttttatcct cttcaaaagc aatggctaca gcacgttcat aaacagcagc attggtattc
     1681 tctttcaaaa tggtaattcc cttgccaaag ttggtcgttt tgggtttaat gacgattgct
     1741 tgtccgctga aaaggctaaa atcggacagg gcggtttgca catcggaata actcattccc
     1801 ttgggcaccc gtattccttg gctggccagt attttctttg tcactacttt gttttccatg
     1861 gccaggattc ctgcataatt atcgagcgat gttttggttg cctgctgaat gtattgcaca
     1921 tttccgtcgc ggctaaggcg aacaaagttc tcatcacggt caagtatttc aaatgcaaca
     1981 cctcttctga ctgcctcgcg caacacaagt tgggtcgaaa gttcaaggtc ttcaaaccct
     2041 ctaaagctga aactgttttt ttgactatcg gccaaatact gacgggcttt ttcgaggtga
     2101 aatgcaatgt accctttcgc ttcagttcct gcaataatct ggtgcacatc ccgctttgca
     2161 ggatc
//
LOCUS       DAOPUW010000327         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_54550_length_2161_cov_2.861823, whole genome shotgun sequence.
ACCESSION   DAOPUW010000327 DAOPUW010000000
VERSION     DAOPUW010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54550_length_2161_cov_2.861823"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /locus_tag="PKJ43_06185"
     CDS             <1..1468
                     /locus_tag="PKJ43_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72192.1"
                     /translation="DCDMGPTIIDGMLYFSSLRTETGSNSRFFNILAMPVNEATEKNA
                     LRETIKDLCTDYHDGPLSYCEATGELFVTVSDIDNSSFTSKKKKKKEVPLKIVVYKSE
                     GNKWVYKEDFPFNKPPYSVAHPAINTSGDTLVFSSNQPGGFGNSDLYMSVREKGVWQT
                     PVNLGNKVNTRKREITPFINTDGMLYFSSDGLKGKGSFDIYAINLASSKTGKPINLPK
                     PINGGMKDYGMVIHPNQQYGFMVSNRKKGRNNDAIFALRKKDFTFKTVTPDSVLIKQL
                     DDIFSRKLSEIEEAANQKKEKNEIYNNVNYHLNYKITDANNFPDLTISCWYTLANDTL
                     RFGKNCYNMGKYNISDSNTGALMLSMLKECIEEDLSTYFTAGRKTNISIHSEPDALPG
                     WEKEYYDGEWGNNLNEMVMINHQAEFVEINNDSILDDTSLAFLRSYATRSFLSEEIDM
                     LHHTRNTFTHHLTKKSDINSNYEWNKIEITIHNAFANF"
     gene            complement(1500..>2161)
                     /locus_tag="PKJ43_06190"
     CDS             complement(1500..>2161)
                     /locus_tag="PKJ43_06190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNZ72193.1"
                     /translation="NIKLVINQLKERNIDPRVFNRCVGDIFFNMLAIKFREEKILFPE
                     LLLSLPDNQLKTMKKEAIELGFPYFTPENREVVLPSGNAVDGLVNLGTGQLSVDQVCM
                     IFNHLPVDITFVDENGKVRYFSSPKKRIFPRTTAVLGREVQNCHPPESVHVVLKIVDS
                     FRSGEKDSATFWIRMKGELILIQYFAVRDESGIYRGIIEVTQEITEIIAIKGEKRLLE
                     W"
BASE COUNT          748 a          446 c          391 g          576 t
ORIGIN      
        1 agattgcgat atggggccaa ccataatcga tggaatgtta tacttctcgt cgttacgcac
       61 agagacggga agcaacagtc gttttttcaa cattttagct atgcctgtaa acgaggccac
      121 cgaaaaaaac gccttacgcg aaaccataaa agatctctgc accgactacc atgatggtcc
      181 cttatcttat tgcgaagcaa ctggtgaact gtttgttaca gtatcagaca tcgacaactc
      241 atcttttact tcgaaaaaaa agaaaaagaa agaagtacca ctgaaaattg tggtttataa
      301 aagtgaagga aacaaatggg tttataaaga agattttccg tttaacaaac cgccatatag
      361 tgttgctcat cctgcaataa acacctcggg cgatacgttg gttttcagca gcaaccaacc
      421 cggaggcttc ggaaattcag acttgtacat gagtgttcgt gagaaagggg tatggcaaac
      481 acctgtgaac cttggcaaca aagtgaatac ccgcaaacga gaaatcactc cttttatcaa
      541 caccgacgga atgttatatt tttcgtcaga tgggttgaaa ggaaaaggca gtttcgacat
      601 ttatgcaatc aacctggctt cgtcaaaaac aggtaaacca ataaacctgc ccaaaccaat
      661 aaatgggggg atgaaagatt atggcatggt aatccatcca aaccaacaat atgggttcat
      721 ggtatcgaac cgaaaaaaag gaaggaataa cgatgccata tttgccctac gaaaaaaaga
      781 tttcacattt aaaacagtaa ctcccgattc tgtgttgata aaacaactcg acgatatttt
      841 ttcgcgcaaa ctgagcgaaa tagaagaagc tgctaatcag aaaaaagaaa agaatgaaat
      901 ttacaacaat gtaaattacc acttgaatta taaaataacc gatgcaaaca attttcccga
      961 cctcacaata agttgctggt acacattggc taacgacacc ttaagatttg gaaagaattg
     1021 ttataatatg gggaaatata acattagcga ctcaaacaca ggagcattga tgctttcgat
     1081 gttgaaagaa tgtattgaag aagacttatc aacatacttt actgccggac gaaaaacaaa
     1141 catctcgata catagtgaac ctgatgcatt accaggatgg gagaaagagt attacgacgg
     1201 agaatggggc aataacctaa atgaaatggt catgataaac catcaagccg aatttgtaga
     1261 aattaacaac gattccatac tcgacgacac cagccttgcc tttctacgtt catacgcaac
     1321 aaggtcattt ttatcagaag aaattgatat gctgcaccat acacgaaata catttacaca
     1381 ccatcttact aagaaaagtg atatcaactc gaattatgaa tggaacaaaa tagaaataac
     1441 aatacacaat gcttttgcta atttttaaat gaagctattc tgctgaaaac taaacttttt
     1501 caccattcaa gaagtcgttt ctcaccttta atagctataa tttcggttat ttcctgcgtc
     1561 acttcaataa ttccacgata aatcccagac tcatctcgta cggcaaaata ttgaataaga
     1621 atcagttccc ctttcattct gatccagaaa gttgcactgt ctttctcacc cgaacggaac
     1681 gagtcaacaa ttttcaatac tacatgcaca ctttcgggcg ggtgacaatt ctgcacttcg
     1741 cgccccaaaa cagctgttgt cctgggaaag attctcttct ttggagaaga aaagtaacga
     1801 actttcccgt tttcatcaac aaaggtaata tctactggca aatgattgaa tatcatacaa
     1861 acttgatcga cactcaattg gcctgtacct aaattcacca acccatcaac tgcattacct
     1921 gagggcaaca ctacttctct gttttcgggc gtgaaatatg gaaaaccaag ttcgattgcc
     1981 tctttcttca tggttttcaa ttggttatcg ggcaatgaca aaaggagttc gggaaacaga
     2041 attttttctt cgcgaaactt aatagccagc atgttaaaga aaatgtctcc tacacagcgg
     2101 ttgaacactc ttgggtcgat attccgctct ttcaattgat taattaccaa tttgatattc
     2161 c
//
LOCUS       DAOPUW010000328         2158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_54665_length_2158_cov_4.090823, whole genome shotgun sequence.
ACCESSION   DAOPUW010000328 DAOPUW010000000
VERSION     DAOPUW010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54665_length_2158_cov_4.090823"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PKJ43_06195"
     CDS             complement(<1..680)
                     /locus_tag="PKJ43_06195"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNZ72194.1"
                     /translation="MKKLILTIVSIVFLLATAMSQIPSSTKPGTEVPLAPRSGVVKGK
                     IVEKGSNVALEYANVAIYKSADSSLVSGSIANDLGTFKLTGLDQGEYYVEVKFIGYDK
                     LSLNNISVTKNQMQVDLGEIQLAPSSENIKEVKVVAQNKAIAYEIDKKVIDPSQFPTS
                     ANGTAVDVLANSPSVSVDIEGNVSLRGSSNFTVLIDGRPTPFTAADALEQIPASTIRN
                     IEVITNPSA"
     gene            complement(842..917)
                     /locus_tag="PKJ43_06200"
     tRNA            complement(842..917)
                     /locus_tag="PKJ43_06200"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(880..882),aa:His,seq:gtg)
     gene            1083..>2158
                     /locus_tag="PKJ43_06205"
     CDS             1083..>2158
                     /locus_tag="PKJ43_06205"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNZ72195.1"
                     /translation="MKKFKLSNVRALQLFQLIRYGSLFLIGILFSKSDFGQTNIGQYE
                     TFTLLAGAFTFFWINGFLKVMMPLSAGKSEDEQKVLIFNSALLIMFFSVLAAILSFSL
                     STPFSSILLNGNQVPMPLVLSGYILFNSPSLIIEYIYLINNKPKKTVAYGTIVFGLQI
                     VIVGLPPFLGYGLNVVLLALLAISVLKFIWMIIVLLQYAKIKIDRTIIREYLSTGTPL
                     VISTLLGSSASYIDGFIVTSKFSPDELAIFKYGAKELPLALLLANSLNMAMLPKLAVK
                     DMRKALSEFRSEVYRLHWALVPLTVVLIFSSHWLFPIIFNPKFQESASIFNITLMLVI
                     SRLLFPQTLLTAQKETRVQVWASL"
BASE COUNT          630 a          465 c          392 g          671 t
ORIGIN      
        1 gctgagggat tagtaatcac ctcaatattg cgaattgtgc tggcaggaat ttgttcgaga
       61 gcatcagcag ctgtaaaagg cgttggacgc ccgtcgatga gcacagtaaa atttgaactt
      121 ccccggaggc taacatttcc ttcaatgtcg accgaaacag aaggagaatt agccaacaca
      181 tcaacagctg taccattggc cgacgttggg aactgagaag gatcgataac tttcttgtcg
      241 atttcgtagg caatagcttt gttttgcgca acgactttta cttccttaat attctcggac
      301 gaaggagcca gttgaatttc accaagatcg acctgcatct ggtttttggt tacagaaatg
      361 ttgttaagag acaacttgtc gtacccaata aattttactt caacataata ttccccctga
      421 tcaagccctg tcaacttaaa cgttcctaaa tcgtttgcaa tagacccgct aaccaaagag
      481 gaatcggctg atttatagat tgcaacattt gcgtattcaa gagctacatt actgcccttt
      541 tctacaatct tccccttaac aactcctgac cggggagcaa gtggaacttc ggtgcctggt
      601 ttagtgctgg aagggatctg agacatggcc gtagccaata aaaatacaat tgacacaatg
      661 gtcaaaatca actttttcat acaatttatc tatttaaaat ctaactctaa ctaataaaaa
      721 tgaacgctag gtagactttt aaacaacttg aaagtttaat gagtttacct acagaaagca
      781 tatttaacta cgttttaaca atacaaaaaa aatcggccag aaactgaccg attattcatt
      841 tgggtgaaag atggggctcg aacccacgac cctcggaacc acaatccgat gctctaaccg
      901 actgagctac attcaccatg ttcgcacttt ctaaaaatgc ggtgcaaata tagtatttta
      961 ttttatttga aaacactcat tgtccaaatt tcaacttttt ttgtttaacg gcttatttct
     1021 tgtcacaata aaatcttcgg tgaaatgttc tatttttgca caacatcaat ttatatttcc
     1081 gattgaaaaa atttaaacta tcgaatgtca gggcacttca gctcttccaa cttatcaggt
     1141 atggttcgct gttcctgatt ggtattctgt tctcaaaatc ggattttggg caaacaaaca
     1201 ttggccaata cgaaacattc acccttttag ccggtgcatt tacttttttt tggatcaacg
     1261 ggttcctgaa agttatgatg cccctttcgg ctggaaaaag cgaagacgaa cagaaagtat
     1321 tgatattcaa ttctgctctt ttaatcatgt ttttttcagt tttggctgcc atactttcgt
     1381 tcagcctgag taccccattt tcttcaatat tactcaacgg caaccaagtg cccatgcctt
     1441 tagtgctaag tgggtacatt ctttttaata gtccatcgct gattatcgaa tacatctatc
     1501 tgataaataa taaacctaaa aaaacggtag cttacgggac aatcgttttc gggttacaga
     1561 tagtaattgt aggcctccct ccttttctgg gatacgggct caatgttgtt ctattagcat
     1621 tactggcaat cagtgtcctg aaatttattt ggatgataat cgttttgctt caatatgcaa
     1681 aaatcaaaat cgatcgaaca ataatccgcg aatatttatc aacaggaacg cctcttgtaa
     1741 tctctaccct tttaggttca tcggccagtt atatcgatgg gtttattgtc acctcaaagt
     1801 tctcgcccga cgaactggct atatttaaat atggagcgaa agaacttccc cttgctctac
     1861 ttttagccaa ttcactcaat atggccatgt taccaaagtt ggcagtaaaa gacatgcgca
     1921 aagccctgtc cgaattccgt tcagaggtgt accgcctgca ttgggcactt gtaccattaa
     1981 ctgtagtact tattttcagt tcgcattggc tattcccaat cattttcaat cctaaatttc
     2041 aagagagtgc ttcaatcttc aacataacac taatgctggt tatcagtcgg ctactatttc
     2101 ctcaaacgtt gctgacggct caaaaagaga cccgggtaca ggtatgggca tctctttt
//
LOCUS       DAOPUW010000329         2155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_54791_length_2155_cov_2.775714, whole genome shotgun sequence.
ACCESSION   DAOPUW010000329 DAOPUW010000000
VERSION     DAOPUW010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54791_length_2155_cov_2.775714"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1577)
                     /locus_tag="PKJ43_06210"
     CDS             complement(<1..1577)
                     /locus_tag="PKJ43_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HNZ72196.1"
                     /translation="MNKFTNVTVSLARIFPPVLLLIVFSPLVVGLVIDPKLIEATNVT
                     INLVWVSLFTIPNLWFKRKWIYRFTLSVFFFFGFLELLHWIIMKGPITLTSLMVMSNT
                     NAQEAVEFMGTKANLGLLIIVPYVLCYIYALRKVPSFQFAGVTKVVVPIVLLFSVGFI
                     AESLINQRFIRKATPPVVRVFLSFVTQQKLFNEAMQANSPRNVAVTPVFDSASQTFVL
                     ILGESCNRNHMSLYGASPKTNPLLEKRTDIITYNNVVSPYSCTIYSVLSILSDASLDQ
                     PISPEKSIDLIDVFHSAGFKTFWISNQSPVGVWDNQVTVLARKTDSPVFVNTTGNSSF
                     EATQTISYDSKLFVPFAQALADDAPKKFIVLHLMGSHTAYDKRYPQEFNVFKGGNHRE
                     RVIARYKNSILYNDFVVDSLLNMVQRQNAGSSGHLASVVYLADHAENVYDEGNNAGHN
                     YTNRLPKANVEIPFVVWLSPAYTSQFSAKVNTLKANRNMPFVADDFFHAALDLSGITC
                     SQFVESRSIFNEHFNASR"
BASE COUNT          637 a          461 c          456 g          601 t
ORIGIN      
        1 cggctggcat taaaatgctc gttgaaaatg cttctcgatt caacaaactg actgcatgtt
       61 attccgctta aatccagggc ggcatgaaaa aaatcgtcgg caacaaaggg catatttctg
      121 ttcgccttca aggtgtttac tttggcacta aactggctgg tatatgctgg cgacaaccac
      181 accacaaaag ggatttcgac attggctttg ggcaatcggt ttgtataatt gtgccctgca
      241 ttgttgcctt cgtcgtaaac attctcagca tggtcagcca aataaacaac ggatgccaaa
      301 tgcccgcttg aaccggcatt ttgtcgctgt accatattca acaagctatc gaccacaaaa
      361 tcgttgtata ggatggagtt tttgtaccgg gcaatcacac gttcccggtg atttcctcct
      421 ttgaacacgt taaattcctg aggataccgc ttgtcatacg cagtatggct tcccattaag
      481 tgcagcacaa taaacttttt gggggcatcg tcggcaagtg cctgtgcaaa tgggacaaac
      541 aacttgctat cgtacgaaat ggtttgggtg gcttcaaacg acgagtttcc cgtggtattt
      601 acaaaaacgg ggctgtcggt tttcctggcc aaaacagtta cctggttgtc ccacacccca
      661 actggcgatt ggtttgaaat ccagaaagtt ttgaaccctg cggaatggaa aacatcgatt
      721 aaatcaatgc ttttttcagg gctaataggt tggtcgaggc ttgcgtccga taaaatcgac
      781 aataccgaat aaatggtaca cgaatagggc gaaaccacat tgttgtaggt tataatatcg
      841 gtgcgttttt cgagcaaagg attggttttg ggtgaggcac cataaagcga catatgattg
      901 cggttgcacg attcgcccaa aatgagcaca aaagtttgcg aggcactatc aaacacaggg
      961 gttacggcaa cgttgcgggg tgaatttgct tgcatggctt cgttaaacag cttttgctgt
     1021 gtgacaaacg aaagaaagac ccttaccaca gggggcgtgg ccttacgaat gaatcgctgg
     1081 ttgatgagac tttcggcaat aaaccctacc gaaaaaagaa ggacgattgg cactacaact
     1141 ttcgttaccc cggcgaattg aaaagaggga accttacgca aagcatagat gtagcaaaga
     1201 acatacggca caataatcaa caagcccagg ttcgccttgg ttcccataaa ctcaacagct
     1261 tcctgtgcat tggtgttcga catcaccatg aggctggtca atgtgattgg ccctttcata
     1321 attatccagt gcagcagttc caaaaaacca aaaaagaaga aaaccgacaa agtgaaacga
     1381 tatatccatt ttcttttaaa ccataagttg ggaatagtga agagggaaac ccataccagg
     1441 ttgattgtta cattggttgc ctctatcagt ttcggatcaa ttacaagacc aacaaccaag
     1501 ggagagaaaa caattaacag cagaaccggt ggaaagatac gggccagcga aacggttaca
     1561 ttggtaaact tgttcatcaa attatggtta acgcttgatt ctttgaaaat aagatcagca
     1621 tacagtaatt gctcaagaat tcaaaaatag ataaaaagga atcaatcagg aaagttgggg
     1681 tgataaaata tgaaaccaac gctccccagc acactcattc gtatgagcaa aaaggatttt
     1741 atacaaaaaa gatttagcat gcttgattct ttgcctcaag ccggctggaa acttttacct
     1801 gagttcctaa gtattcagga ataaagtctt tatactttgt actttatact ttgtacttta
     1861 tactttgtac tttatacttt gtacttgtgt ctttttttga gtgccttagt ttcttcccat
     1921 tgttttcaat tgtctcctat cgccttctat cgttttcaat cgccttccat gctttccttt
     1981 gtgcttgcgt gtttttcatt gtcaattgtc aattatcaat tatcaattgt tcttaatatc
     2041 tttaccccaa aacaatcagc aacaatgata ataccgccct atcttcagcc tggcaatacc
     2101 ataggaattg cttccccggc gtcgaaaatc gaccggcaag atgtggaacc agcca
//
LOCUS       DAOPUW010000330         2153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_54857_length_2153_cov_3.612011, whole genome shotgun sequence.
ACCESSION   DAOPUW010000330 DAOPUW010000000
VERSION     DAOPUW010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54857_length_2153_cov_3.612011"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PKJ43_06215"
     CDS             <1..711
                     /locus_tag="PKJ43_06215"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNZ72197.1"
                     /translation="NQSIAVPDYKGMSHKEYVSITKKKHLRYEIIDSIYVEEAQPGTV
                     IEQFPSAASKVKRKRTIYLTLAAVSPEKVPMPKVTDVSLREAQSRLENIGLKVGNVSY
                     RPSEFYNLVLEQRYKNKVVAKGEMIPEGTAIDLIVGKGLSGEKTELPVLMGLTIESVR
                     SILFYNNLSVGALVYDNSILTSSDSLNAKVWKQLPDPLKTEFIELGSSVDLWLTIDEE
                     KLNPTAESDTLFDDSGDF"
     gene            692..1738
                     /locus_tag="PKJ43_06220"
     CDS             692..1738
                     /locus_tag="PKJ43_06220"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNZ72198.1"
                     /translation="MTQEIFDEIDENEDGGLFEHFRYEVDPGQKMLRIDKFLVNRMEG
                     TSRNRIQAASDAGCIMANGQPVKSSYKVKPNDIITIVLDYPKRELKIIAEDIPLNVVF
                     EDEHLLVVNKPAGLVVHPGHGNYTGTLVNALAYRFKGLPLFNSEDPRPGLVHRIDKDT
                     SGLLVVAKTEQAKLHLSMQFYNKTTKRAYQALIWGIPVQDEGTVTGNIGRSPKNRQVF
                     TVFPDGEQGKHAVTHYSILEKLGYVCLVECRLETGRTHQIRVHMKHLGHPLFNDATYG
                     GNEILRGTTFTKYKQFVQNCFSICPRQALHAKSLGFIHPATGEEMYFESELADDMKLL
                     IEKWRTYTSSRSEE"
     gene            1829..>2153
                     /locus_tag="PKJ43_06225"
     CDS             1829..>2153
                     /locus_tag="PKJ43_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72199.1"
                     /translation="MKSELLLFTLIVITLVTSCTTNRTSGEQSFMLKENWKIQESAKA
                     KASGQAISMAEYNTRDWIDARVPSTVMGILSTQEKYANLFVDNNYSKADKSIFDSSWW
                     YRTTFE"
BASE COUNT          668 a          474 c          451 g          560 t
ORIGIN      
        1 aaccaatcaa ttgcagtgcc cgattacaaa ggaatgtcgc ataaagaata cgtttcgatt
       61 accaaaaaga aacacctgag gtatgaaatt atcgattcta tttatgttga agaagcacag
      121 ccgggtacgg taatagagca atttccttcg gctgcttcga aagttaaaag aaaaagaacc
      181 atatacctca ctttagcagc ggtttctccc gaaaaggttc caatgccaaa agtaaccgat
      241 gtttcgttgc gagaagcaca aagcaggctc gaaaacatag ggttaaaagt tggcaacgta
      301 agctaccggc cttcagaatt ttacaatctt gttcttgagc aacgttacaa aaacaaagtt
      361 gtggctaaag gggagatgat tcctgaagga acagcaattg atcttattgt tggtaaaggg
      421 cttagcggtg aaaaaaccga actgccagtt ttgatgggac ttaccattga aagtgtccgg
      481 tcaatacttt tctataataa tttgagtgta ggcgctttag tatacgataa ttcaatttta
      541 acttcttccg attcgctgaa tgccaaggta tggaagcaat tgcccgaccc gttgaaaacc
      601 gagtttattg aattgggatc atcggttgat ttatggctta ctatcgacga agaaaaatta
      661 aaccctaccg cagaatcaga tacccttttc gatgactcag gagatttttg acgaaattga
      721 cgagaacgaa gacggcgggt tattcgagca tttccgttac gaagtagatc ccgggcaaaa
      781 aatgctccgg atagataaat ttctggtcaa ccgcatggaa ggtacatcgc gtaaccgtat
      841 ccaggctgca tctgatgcgg gttgtatcat ggctaacggg cagcctgtta aatcaagcta
      901 taaagttaaa cccaacgata ttatcaccat tgttctcgac tatcccaaaa gagaactaaa
      961 aattatcgcc gaagatatcc cgcttaatgt tgttttcgaa gatgagcact tattggttgt
     1021 caacaaacct gccgggcttg ttgtacaccc aggacacggg aactacaccg ggactcttgt
     1081 caacgctctt gcttaccgtt tcaaaggact gcccctgttc aattcagaag acccgcgccc
     1141 gggccttgta catcgtattg ataaagatac ttcagggctg ctggtggttg ccaaaaccga
     1201 acaagccaag ctgcacctgt ccatgcaatt ctacaataaa acgaccaaac gggcctatca
     1261 ggcactcatt tggggaattc ccgttcaaga tgaaggcacc gtaaccggga acattggccg
     1321 cagtccaaaa aaccgacagg ttttcaccgt tttccccgat ggtgagcaag gcaaacatgc
     1381 tgttacacat tactctatcc tcgaaaaact gggttatgtc tgcttagtcg aatgcaggct
     1441 cgaaaccggc cggacgcatc aaataagggt acacatgaaa cacctgggac acccgctttt
     1501 taacgatgca acttacggcg gtaacgaaat cctcagggga accaccttta caaaatacaa
     1561 gcaatttgta caaaactgtt tttcaatttg cccgcgccag gccttgcatg ccaaatcgct
     1621 ggggtttatc catccggcta caggcgaaga aatgtatttt gaatcagaac ttgccgatga
     1681 catgaagcta cttattgaaa aatggcgcac ttacacttct tcgcgctccg aggaataaac
     1741 aaactcaccc ttcttctttt ttttatcggc ccttaatttc ttattataaa ttctgctaag
     1801 tttagaggat aattcaatat cgacagaaat gaaaagtgag ctactccttt ttacattgat
     1861 tgtgattact ttagtaacaa gttgtacaac aaaccgaacc tcaggagaac aatcattcat
     1921 gctgaaagaa aattggaaaa tacaagaatc agctaaggca aaagcctcag gacaagcaat
     1981 ttccatggca gaatataata cccgcgactg gattgatgcc cgcgtaccat caaccgttat
     2041 gggcatatta tcaactcagg aaaagtatgc caatcttttt gttgacaaca attactctaa
     2101 agcagacaag tcgatatttg actcttcgtg gtggtatcgc accacttttg agg
//
LOCUS       DAOPUW010000331         2152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_54890_length_2152_cov_3.422031, whole genome shotgun sequence.
ACCESSION   DAOPUW010000331 DAOPUW010000000
VERSION     DAOPUW010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54890_length_2152_cov_3.422031"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PKJ43_06230"
     CDS             <1..415
                     /locus_tag="PKJ43_06230"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNZ72200.1"
                     /translation="VRNMGGGYYNHNLFWENLSPTPGKLSKELQTELEKQFGSVEAFK
                     EAFSKASSSVFGSGWTWLVVTTDGKLEITSTANQDNPLMDVASKKGTPLLTIDVWEHA
                     YYLKYQNKRADYITAFWNVVNWEEVSKRFEKAKGK"
     gene            complement(503..1498)
                     /locus_tag="PKJ43_06235"
     CDS             complement(503..1498)
                     /locus_tag="PKJ43_06235"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNZ72201.1"
                     /translation="MSEKNRYSEQFDVILRYMNNTMTNEERYKFERELERDPFLYEAF
                     DGLSGLKPAEAERAIRSIDVISGKRHLSFRFLKYIGYVAAVAIIVVGLFWGFQQIDFS
                     GKNVGSNTVQNEDAVLMEPYRPAKSDSIDSIGNQEDVSSADLLMSEPNGTKTESTAQN
                     TTPVNKEPAKKKQSLQNAGQQVAQKPSEEKEQQALTDIKSPDETEVDAEETSASKEEY
                     LPEDIEKPEEALKRPGVNAEPEPLGGSSLFKSYLDNNARYPESADSKRKEIVKIRFRV
                     SKTGDPSNFFIERSPGDAFSQEAIRLIKNGPKWSPQIKDGLPVDGEVSVRITFKP"
     gene            complement(1482..2075)
                     /locus_tag="PKJ43_06240"
     CDS             complement(1482..2075)
                     /locus_tag="PKJ43_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNZ72202.1"
                     /translation="MPSTLKQLEFDRQLDLLQIREFRKTGNLEILGNIYAKYMHLVFG
                     VSMKYLKNKEAAKDAVTQVFEKLTIEAVRHEIVHFRNWLYVVTKDHCLTELRKQNLED
                     EQFANWAEEEKKIMESGLEVHPIDEQDRLGEALHDCINKLSLQQRDCVDLFYLKKKSY
                     REIALLMKYDEKSVKSYIQNGKRNLKICLELKDEREK"
BASE COUNT          611 a          469 c          392 g          680 t
ORIGIN      
        1 tgtgcgcaat atgggcggtg gatattacaa tcacaacctg ttctgggaaa acctttctcc
       61 taccccaggg aagctttcaa aggaattaca aacagaactt gaaaagcagt ttggatcggt
      121 agaagcgttt aaagaagcgt tcagtaaagc atcgtcttct gtgtttggca gtggttggac
      181 atggcttgtg gtaacaaccg atggaaaatt ggaaattacc tcaacggcaa atcaggataa
      241 cccgttaatg gatgtagcct cgaagaaagg aactccgcta ctaacgatcg acgtttggga
      301 gcacgcctat taccttaaat atcaaaataa aagagcagat tacattactg ctttctggaa
      361 tgtcgtgaat tgggaagagg taagcaaacg cttcgaaaag gctaaaggaa aatgatataa
      421 attccttgag ataagcataa aaaaaaggag taagtcaata gggactcact ccttttttca
      481 ttcctgactt tatattgttc tcttatggtt taaaggttat ccgcactgaa acttcaccat
      541 cgacaggaag cccgtctttg atttgaggag accacttagg accattcttt atcaaacgga
      601 ttgcttcttg tgaaaatgcg tcaccaggac tgcgttcaat gaaaaaattc gatggatcgc
      661 cagtcttcga caccctgaac cgaattttta ctatttcttt ccgcttacta tcagccgatt
      721 ctgggtaacg ggcattatta tcgagataac tcttaaagag tgaacttcca ccaagtggtt
      781 caggttcagc attcaccccc gggcgtttca acgcttcttc cggcttttca atatcttcag
      841 gtagatattc ttcttttgaa gctgaagtct cttcggcatc aacctctgtc tcatctggag
      901 atttaatatc ggttagcgct tgttgttctt tctcttcact tggcttttgt gcaacttgtt
      961 gaccagcatt ctgtaacgat tgtttctttt tagctggctc tttatttact ggagtagtat
     1021 tttgagcagt agattcggtc ttggttccat tgggttcaga catcaacaaa tcggcagaag
     1081 acacatcttc ttgattacca atggaatcaa tcgagtcaga tttggcaggc cgatagggtt
     1141 ccatcaacac tgcgtcttca ttttgaacag tattgctgcc cacatttttc ccgctaaaat
     1201 cgatttgctg aaaaccccag aaaaggccga caacaataat tgcaaccgca gctacatacc
     1261 caatatattt caagaagcga aatgaaagat gccgttttcc cgatattaca tcgatactac
     1321 gtattgcccg ctcggcttca gcgggtttta gccccgacaa accatcgaat gcttcataca
     1381 aaaaaggatc gcgctctaat tccctttcaa atttgtaacg ttcttcgttg gtcatagtat
     1441 tattcatata cctcagaatc acgtcaaact gctcactata tctatttttc tcgctcatct
     1501 tttaattcaa ggcaaatttt caggtttctt tttccgttct gaatatagct tttcacgctt
     1561 ttttcatcgt atttcatcaa caatgcaatt tcgcggtacg attttttctt caaatagaat
     1621 aagtcgacac aatcgcgctg ttgaagtgaa agcttattaa tacaatcgtg aagggcctct
     1681 cctaacctat cctgctcatc aataggatga acttctaagc ccgattccat aattttcttt
     1741 tcctcttcag cccagttagc aaactgctca tcttcgaggt tctgctttcg cagttccgtt
     1801 aagcagtggt ctttggtaac aacataaagc caattgcgaa aatgaacaat ttcatgcctt
     1861 accgcctcaa ttgtaagttt ttcaaacact tgcgtcaccg catctttagc agcttcttta
     1921 tttttcaaat acttcatgct caccccaaaa accaggtgca tatattttgc atatatgttt
     1981 cctaaaatct ccaggttccc agttttccga aattccctga tttgcaacaa atcaagctgt
     2041 cggtcaaatt cgagttgttt taatgttgac ggcattatcg ttgtgctatt aaaccaatgt
     2101 ataaaagtaa caacaaatat ttatcaaaaa aatttatgga attcaaaaat tt
//
LOCUS       DAOPUW010000332         2152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_54897_length_2152_cov_2.618503, whole genome shotgun sequence.
ACCESSION   DAOPUW010000332 DAOPUW010000000
VERSION     DAOPUW010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54897_length_2152_cov_2.618503"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1318
                     /locus_tag="PKJ43_06245"
     CDS             155..1318
                     /locus_tag="PKJ43_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNZ72203.1"
                     /translation="MSGILVVDDERAIRNTLKEILTYEKYTVDLAENAKQALELVKNN
                     EYDIILCDIKMPEMDGLELLPLLVETQPDSPVVMISGHGNIDTAVEAIKKGAYDFIEK
                     PLDLNRLLITIRNAMDKSTLVAETKTLRKKIGSKYTIVGHSPAMNGIMGMVQKVAPTD
                     ARVLITGSNGTGKELVARRIHDLSNRSAGPFIEVNCAAIPSELIESELFGHEKGAFTS
                     AIKQRKGKFEQAEGGTLFLDEIGDMSLSAQAKVLRALQENEITRVGGEKALKVDVRVV
                     AATNKDLQKEIEAGNFREDLYHRLSVILIRVPSLNERLDDIEELANYFSEQICGEYGM
                     PTKTFAPDAIQAFKAINWTGNIREFRNVIERLIILCGNEITKEDVTTFVQPMK"
     gene            complement(1356..>2152)
                     /locus_tag="PKJ43_06250"
     CDS             complement(1356..>2152)
                     /locus_tag="PKJ43_06250"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HNZ72204.1"
                     /translation="FWEKPFDIKKVNKLLSLIPSDASVSSSNMFTPHLAQRQYIYFFP
                     NVKDADYIVFSVSDDNYLYSQIINERYRFDYLSSPKWEIIAAEYPVFLLKRRNEESAQ
                     LNAPLNSIWSHTETQKFSFGDANSSYGNDQDSTIKPSVSVSESIQLKNDNENIAISLH
                     GENSYSPFISIKDLEKINHLRIEVWQETQKKGAAFIVADNSNGFYRSSNVCDSIDSQG
                     RERLVLSFWIPQNIGSDELKIALKNTTNELIHFGDITITKGYKRNN"
BASE COUNT          667 a          372 c          487 g          626 t
ORIGIN      
        1 ttgtttattg cttaaaacaa acaatctttg taaagctaag atgctatatt gaaaatgtga
       61 atcagataaa agtacttaat ttgaatataa atatctgaag ctaaaggttt taattgcaat
      121 cgggtttgtt atttatgtgt aaaatagatt aggtatgtca ggaattttag tagtcgacga
      181 tgagagagcc attcgcaata cgctgaagga aattttaacg tatgagaaat atacggtcga
      241 tttggccgaa aatgccaagc aagcgcttga attggtgaag aataacgagt atgatatcat
      301 tttgtgcgat attaaaatgc ccgagatgga cggtcttgaa ctgttgccgt tattggttga
      361 aacacagccc gattcgccgg ttgtgatgat ttcggggcac gggaatatcg atactgcagt
      421 tgaagccata aaaaaagggg cttacgactt tattgaaaaa ccgctcgacc tgaaccgcct
      481 gctcattacc atacggaatg cgatggataa atcgactttg gtggccgaaa ccaaaaccct
      541 gcgcaaaaaa atcgggtcaa aatatactat tgtcgggcat tcgccagcaa tgaacggcat
      601 aatgggaatg gtacagaaag tggcacctac cgatgcccgc gtactcataa ccgggagcaa
      661 tggtactggc aaggaattgg ttgcccgaag gatacacgat ctgagcaatc gttcggcagg
      721 cccttttatt gaggtgaatt gtgcggctat tccttcagaa ctgatcgaga gtgaattgtt
      781 tggacacgaa aaaggggcat tcacctcggc aataaaacaa cgcaaaggga aatttgaaca
      841 agccgaaggc ggaacactct ttctcgacga gattggggat atgagtttgt cggcacaggc
      901 aaaagtgttg cgtgccctgc aggaaaatga gattactcgc gtgggaggag aaaaagcact
      961 gaaagttgat gtgcgagtgg ttgcggctac taacaaggat ttacaaaagg aaattgaggc
     1021 aggaaacttt agggaagacc tttaccaccg gttgagtgtt attcttattc gcgtgccttc
     1081 gctcaacgag cggctcgacg acattgaaga attggccaac tatttttctg aacaaatttg
     1141 cggagaatac ggtatgccca ccaaaacttt tgctccggat gcgatacaag catttaaagc
     1201 aattaattgg acgggcaata tacgagagtt caggaacgta attgaaagat taataattct
     1261 ttgtggaaat gaaataacaa aggaagatgt aaccactttt gttcagccaa tgaaataggg
     1321 ttaatcaact ttttactaaa cttgttgtat gtaggttagt tatttcgttt atagcctttt
     1381 gtgattgtta tatcgccaaa atgaatcagt tcatttgtgg tgtttttaag agctattttt
     1441 aattcatcgg agccaatatt ttgaggaatc caaaaactaa ggacaagtct ttccctgcct
     1501 tgtgaatcaa ttgagtcgca tacattactt gacctataaa aaccattgct gttatcggca
     1561 acaatgaatg cagccccttt cttttgtgtc tcttgccaga cctctattcg aagatggttt
     1621 attttctcga gatctttaat gctgataaac ggactatagc tgttttcacc atgcagtgaa
     1681 atggcaatgt tttcattatc atttttcaat tgaattgatt cggagacgct cactgatggt
     1741 ttgatcgttg aatcttgatc atttccgtaa gaagaattcg catccccaaa gctgaacttt
     1801 tgtgtttctg tatggctcca tatagaattg agaggagcat tgagttgagc tgattcttca
     1861 tttcttcgtt tcagaagaaa cacaggatat tcagcggcaa taatctccca tttgggagat
     1921 gaaagataat caaagcgata tctctcgttg attatttgag agtacaaata attatcgtct
     1981 gacacagaga aaacaatgta atcagcatct tttacattag ggaaaaagta aatatactgg
     2041 cgttgggcaa ggtgaggggt gaacatattt gatgaactca cgctggcatc agaaggtatg
     2101 agagacaata atttatttac tttttttatg tcaaaaggct tttcccaaaa ac
//
LOCUS       DAOPUW010000333         2142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_55263_length_2142_cov_4.228558, whole genome shotgun sequence.
ACCESSION   DAOPUW010000333 DAOPUW010000000
VERSION     DAOPUW010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55263_length_2142_cov_4.228558"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            380..>2142
                     /locus_tag="PKJ43_06255"
     CDS             380..>2142
                     /locus_tag="PKJ43_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72205.1"
                     /translation="MYKRIYILIVVLFSLVVSSCTKDDDFFSAPGLGRTLIDTTLNVS
                     QREVTVTLSEGITVTVPKGLSSKSFSMKIREVDMSVQTPAVINLLKAFDIELSIGEEF
                     EKEIIIRVDFEDDDLSGDVTSNDLAFGFYNTYNKKWAVFPDYEVNLDKGYAVCRTNHL
                     TTVGVWEFVSSGGYSHKFIGDNITVYYTLRGDDAPMDYSEYLPDDKTWHLPSGHKNWA
                     PIYIQDIAHYTAEARKLFAASPHNLNVSKGNINIYVKDMNGSDGEYGTISGAIYINNT
                     VKIPTNVSGVEGQDVLKATCAHELMHFIQDNYYVMNKGSIGMWWLEATATQADRMAWG
                     NSLTYSESELFAIESNSALLENLSKSWDDCNSNPNWYLAGCFLQYLSTYRMGEKLNVA
                     KAIKQGGSGASLVRVMLNDIIKSDLETSITDEFHDYVLYLFIEGNENLTAFPVDKDFS
                     KIETASSLTKQVKMSKKDNKQTVEVSLPYMSAKLISVSNLENTDMMYSYTYLQDAPGV
                     MAYLCKVDKKNGMLDIVDRLSKDDAGNVELKARVGGDADSFIILLINMNFTEGKKTVK
                     YIFETAPDFKDFDYMSFKLEG"
BASE COUNT          606 a          386 c          459 g          691 t
ORIGIN      
        1 ttctcttttt ttgtgcgttc aaatatgcca cactaccacc aaacacttgt gtgctaagct
       61 tactatgata aacagcttgt tgaaaaattt gttcccaatg tttgggctga atttccctct
      121 gtctgtaatg cctcaaattt agcttcttaa tttcattttt taatgcgata attatttttt
      181 ttacactata gattgatctg tttcttgaat gcgcattatg tttttctgaa ttgttttaag
      241 cagatcattc ttttctgttt tgttattttt aaatatttct ttgtatcttg ctctctgaat
      301 attactcatg tggctattag tcatgtactt attggtttgt tgtctccaat tctttttcag
      361 aaacttaaaa attaccgtca tgtacaaaag aatttatatc cttatcgttg ttctgttttc
      421 gctcgttgta tcttcttgca ctaaggacga tgattttttt tcggctccgg gcttggggcg
      481 taccttgatc gatacaacat tgaatgtttc gcaacgcgag gtcacggtaa cgctttctga
      541 gggtatcacg gttacagttc ctaaggggct ttcaagcaaa agtttttcaa tgaaaattcg
      601 cgaagttgat atgtcggtac aaacccctgc tgtaattaat ctcctgaaag cgtttgatat
      661 tgaattaagt attggcgaag agtttgaaaa ggagataatt atacgtgtcg attttgaaga
      721 tgacgatctt tcgggcgatg taacttccaa cgatttagcc ttcggttttt ataatactta
      781 caataaaaaa tgggctgtat ttcccgacta tgaggtcaat ctggacaagg gctatgctgt
      841 ttgtcggacc aatcacctta ccactgtggg tgtgtgggaa tttgtttcat cgggtggcta
      901 ttcacataag tttataggcg ataatattac cgtgtattac accttgcgtg gtgacgatgc
      961 cccgatggat tacagcgaat atttgcccga tgacaaaaca tggcatttgc cctcgggtca
     1021 taaaaactgg gcacctatat acattcagga tattgcccat tataccgccg aagcccggaa
     1081 gctttttgcg gccagccctc ataacctcaa tgtttcgaaa ggaaatatta atatctacgt
     1141 aaaagatatg aatggaagcg atggcgaata tggcaccatt agcggggcaa tttatatcaa
     1201 taatacagtg aaaataccaa ccaatgtgtc gggggttgaa ggtcaggatg tactgaaagc
     1261 tacttgtgca cacgaattga tgcactttat tcaagataat tactacgtga tgaacaaggg
     1321 cagtataggt atgtggtggc tcgaggcaac tgcaacacag gccgaccgga tggcgtgggg
     1381 caattcgctc acatacagtg agtctgagct gtttgcgatt gaatcaaatt ctgccctgct
     1441 cgagaacttg agcaagtcgt gggacgattg taactcgaac cccaactggt atcttgctgg
     1501 ctgctttctg caataccttt caacctatcg tatgggcgaa aaactcaatg tggctaaggc
     1561 aattaagcaa ggagggagtg gggcaagttt agttcgggtc atgctaaatg atataataaa
     1621 atcagacctg gagacatcga tcaccgacga atttcacgac tatgtccttt atcttttcat
     1681 cgaaggaaat gaaaacctta cagctttccc tgttgataaa gattttagca aaattgaaac
     1741 tgccagttcc ttgaccaaac aagtgaaaat gtcgaaaaaa gataataaac aaacggttga
     1801 ggtttcactt ccttatatgt cggccaaact tatcagtgta agcaatttag agaatacgga
     1861 tatgatgtac agttatactt atctacaaga tgctccaggg gttatggctt atttgtgcaa
     1921 ggtagataag aaaaatggga tgctcgatat tgttgatcgg ttatcgaaag acgatgctgg
     1981 taatgttgaa cttaaggctc gtgtgggtgg cgatgccgat agttttatta tcttgcttat
     2041 taatatgaat tttacagagg gaaagaaaac tgttaagtat attttcgaaa cggctcctga
     2101 ttttaaagat ttcgattaca tgagctttaa actcgaagga ga
//
LOCUS       DAOPUW010000334         2141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_55334_length_2141_cov_2.594439, whole genome shotgun sequence.
ACCESSION   DAOPUW010000334 DAOPUW010000000
VERSION     DAOPUW010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55334_length_2141_cov_2.594439"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..377
                     /locus_tag="PKJ43_06260"
     CDS             21..377
                     /locus_tag="PKJ43_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72206.1"
                     /translation="MSVSYITRNNKRVLFIEYKDCKSVEDTLRVLDQIKTEFLKTEGI
                     WLTLHDFSNGFGSNEFMKKASQYANEYFNTRPALNAAIGVSGLKKILLQGYNLVVKDK
                     IVPFNTMDEALNYLTR"
     gene            403..1149
                     /gene="mtgA"
                     /locus_tag="PKJ43_06265"
     CDS             403..1149
                     /gene="mtgA"
                     /locus_tag="PKJ43_06265"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNZ72207.1"
                     /translation="MTISEAIFKKRAKKSNRNRSLLGRIFFFFKWILFLLFFSSLFVV
                     FVYKFINPPVTPLMAIRCVQQVSKGEKIILKKDWTRIEEISPNMIKAVIAAEDNLFVT
                     HKGFDKEAIEQAIEYNKKGKKIRGGSTISQQTAKNVFLWPSRSYIRKGLEAYFTFLIE
                     FMWGKQRIMEVYLNVIEIGNGIYGVEKAASVYFQKSSATLTAGQAALIAAALPSPRRY
                     SITNPGPYMRSRQEQLINLMPKIETLKIGQ"
     gene            complement(1211..>2141)
                     /locus_tag="PKJ43_06270"
     CDS             complement(1211..>2141)
                     /locus_tag="PKJ43_06270"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ72208.1"
                     /translation="LKKIPIDDLLSHIEIVESGLNDFSANKLPKGIKKYTVIHAAASV
                     NLGVNEHSGIEIEENNFKGTLRLLHEITPFASQFVYVSTAYSSGHRAGNIDNDFLSLE
                     KFDFRNHYEFFKNKTEKYIEQYCPLHGIDWKIFRPSIICGRLIDAPYYAISRFMVFYL
                     FARYAVAMKSRMDGAKLRLHVPENAQINIVPVDFVAKSIIEGLSSPIQQLNIVHPENV
                     SCRSLFKNGFGLIDFTRYTFLETMPLPENPVEKMLYSTVGQQLGPYIDTPSHHFDTSA
                     LKKLMPDFSVPDIEPHFGGLLEFAANRKFAPLY"
BASE COUNT          704 a          405 c          432 g          600 t
ORIGIN      
        1 taaattaaat aaacatcatc atgtcagttt catacatcac ccggaataac aaaagagtac
       61 tttttattga gtacaaagac tgtaaatcag ttgaagacac attaagggtt ctcgaccaaa
      121 ttaaaacaga gtttcttaaa accgaaggta tctggttaac attgcacgat ttttcgaacg
      181 gttttggaag taatgaattt atgaagaaag cctctcaata tgcaaatgaa tacttcaaca
      241 cacgtcctgc attgaatgca gccataggtg tttcggggct taaaaagata ttattgcaag
      301 gatataatct ggttgtaaaa gataaaatcg tacctttcaa cacaatggac gaggcgctga
      361 attacctgac aaggtaagta taagcaacac agataagaaa gcatgacgat aagcgaagca
      421 attttcaaaa aaagagcaaa aaagagcaat aggaatagat cgctgttggg gcgtattttc
      481 ttctttttta agtggatatt gttcctcctg tttttttctt ctttatttgt tgtattcgtt
      541 tacaaattta ttaatccgcc agttacccca ttgatggcaa tccgttgtgt ccagcaagtt
      601 tcgaaaggag aaaaaattat ccttaaaaaa gattggaccc ggattgaaga aatttcgcca
      661 aacatgatta aagccgtaat tgcggcagaa gataatcttt tcgttacgca caagggattc
      721 gacaaagaag cgattgaaca agccatagaa tacaacaaga aagggaagaa aatacgaggc
      781 ggaagcacca ttagccaaca gacagcaaag aatgtttttc tatggccatc acggtcatat
      841 atcaggaaag gtttggaagc ttatttcacg ttcctcatcg aattcatgtg gggaaaacag
      901 cggatcatgg aagtatacct gaatgtaatt gaaatcggga atggaattta cggagtagaa
      961 aaagccgcgt cggtatattt tcaaaaaagc agcgcaacct taaccgccgg tcaagcggca
     1021 ttgattgcag ctgcattacc ctctccacgc cggtatagta ttacaaaccc tggtccatac
     1081 atgcgttcaa gacaagaaca gttaattaac ctgatgccca aaattgaaac cttaaaaatt
     1141 ggccaataac ttatcgtcaa aatcttacgt caaattcaga cagtatgctg tgttttaatt
     1201 acttacttta ttagtataaa ggagcaaatt ttctgtttgc agcaaattca agtaagccac
     1261 caaaatgggg ttctatgtct ggtaccgaaa aatcgggcat cagctttttc aatgctgatg
     1321 tatcgaaatg atgggaaggc gtatcgatat atggtcccag ttgttgacca acagtactat
     1381 agagcatttt ctcgacaggg ttttcgggta gaggcatagt ctcaagaaag gtataccggg
     1441 taaaatcaat aaggccaaac ccatttttaa agagcgaccg acacgaaaca ttctctggat
     1501 gcacaatatt tagctgttga ataggcgaag agaggccttc gatgatcgat ttggcaacaa
     1561 agtcgacagg aacaatgttt atctgtgcat tttccggaac atgcagtctc aatttagccc
     1621 cgtccattcg gcttttcatg gccacggcat agcgtgcaaa aagataaaat accatgaaac
     1681 gtgaaattgc ataataaggg gcatcaatca acctcccgca tattatcgat ggcctgaaaa
     1741 ttttccaatc gatcccgtgc aatgggcaat attgctcaat atacttttcg gttttattct
     1801 tgaaaaactc ataatggtta cgaaaatcaa atttttccaa cgagagaaaa tcattgtcga
     1861 tatttcctgc acgatgcccc gaagaatagg cagtgcttac atacacaaat tgggaagcaa
     1921 aaggggttat ctcatgcagc aaacgcaagg tgcccttgaa gttgttttcc tctatctcga
     1981 ttcctgagtg ttcgtttacc ccaaggttta ccgaggctgc tgcatggata actgtatatt
     2041 ttttgattcc ttttggtagt ttatttgcac taaaatcgtt gagcccagac tcgacaattt
     2101 cgatatgaga caaaagatcg tctatgggta ttttcttcaa c
//
LOCUS       DAOPUW010000335         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_55656_length_2132_cov_3.077034, whole genome shotgun sequence.
ACCESSION   DAOPUW010000335 DAOPUW010000000
VERSION     DAOPUW010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55656_length_2132_cov_3.077034"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PKJ43_06275"
     CDS             complement(<1..255)
                     /locus_tag="PKJ43_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72209.1"
                     /translation="MNTYTKKISAKLHALALLLLLVLVGLQPANADVSLPYVLSSNMV
                     VQREKGTTIWGWASSGEKVKVTFRGKTFSCKASKDGHWNVK"
     gene            complement(582..>2132)
                     /locus_tag="PKJ43_06280"
     CDS             complement(582..>2132)
                     /locus_tag="PKJ43_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNZ72210.1"
                     /translation="VGADGANDDSFFYGNGFGTKSETEGNDWITVNQLSLTGYDNQKS
                     VVYEDGGAGNLVWKWVNLSAFSLAETKITFSADEAGKPFTFQIGGREDGLEIDKFVFA
                     PAGLYYTVKNLDNGEEGSVDLPEIDLADQPLAYGLSKYLGCAYASHTKDNFEKYWNQV
                     VPENAGKWGSAEPSRDVMLWGQLDEAYNFALKNNFPYRHHVLVWGNQQPAWIENLPAS
                     EQLEEIREWFQAVANRYPKAEFVEVVNEALHDPPNMPGSGGGNYIEALGGTGQTGYDW
                     IVNAFKMARQYFPATTKLMLNEYGITDNANNARRYKAIIDLLKSDNLIDAVGVQGHAF
                     NTGVSASTIISNLDIVAQTGLPIYITEMDVDGETDAIQLAAYKRIFTTFWEHPAVKGV
                     TLWGFRPGMWRTDQRAFLVNSDGTEREAMTWLRNYLRGFLSADEIQNTPGFTIYPNPV
                     SNGAIQIIGAEEIRELRFFSTSGQLIKSFAADNQHVVSVDIDFMPGIYVVQLIGNKQI
                     TTRKIVVK"
BASE COUNT          592 a          510 c          410 g          620 t
ORIGIN      
        1 cttaacattc caatgtccat ctttcgaagc tttacacgag aaggtttttc cccggaaggt
       61 aacctttact ttttcgccac tcgatgccca accccaaatg gttgtgcctt tttcgcgctg
      121 aacaaccata ttggagctca acacataagg aagactaaca tcggcattgg cgggttgtaa
      181 cccaacaagc acaagcagaa gcaatagcgc taaagcatgc agtttagccg agattttttt
      241 tgtgtacgta ttcatgtttt tttcattaaa gttaaccgca tttgaaccgg catgttctgt
      301 gatgtacaaa tttgaacttt ttatgtcatc aatgcgacct ctaatttatt cactttagaa
      361 ggaatttaga caaataatga gggacaaatc tttcaggaaa attcaacgga gaatattccc
      421 tttttgcttt tttgtcaact ctctattcct gttttctatt ctacaatttg aataatcaga
      481 tcatttggag gataaaaata gacgatatca caaatctgaa aatcagaata ctgcaacatc
      541 ggccatttta ataaaagcct ccttattatc caattttaaa actatttgac cactattttt
      601 cgtgtagtaa tttgtttatt gcctataagt tgaaccacat aaataccggg cataaagtcg
      661 atatcgacag aaacaacgtg ctgattatcg gctgcaaaag attttatgag ttggccactc
      721 gttgaaaaga acctcagttc gcgtatttct tctgcaccaa taatctgaat tgccccattg
      781 gaaaccgggt taggataaat agtgaagccc ggagtatttt gaatttcatc agcagacaag
      841 aacccgcgaa ggtagtttct taaccaagtc atggcttcac gctcggttcc atcggaatta
      901 actagaaaag cgcgctgatc ggtgcgccac attcccggtc gaaaacccca caacgtaact
      961 cctttcactg caggatgttc ccaaaatgtg gtgaatattc ttttgtaggc tgctaactga
     1021 atggcatcag tttctccatc tacatccatt tcggtaatat aaattggcaa accggtctgg
     1081 gcaacaatat cgagattgct tatgatggtt gaagccgaaa cacccgtatt gaacgcatgc
     1141 ccctgaacac caaccgcatc aataaggtta tcacttttca acaagtcgat tatggctttg
     1201 taacggcgag cattattggc attgtcggta atgccatatt cgttgagcat taacttggtt
     1261 gttgccggaa aatattgacg ggccatttta aaggcattca caatccaatc gtatcctgtt
     1321 tgtccggtgc cccccaaggc ttcgatgtag tttcccccac cactgccggg catgtttggc
     1381 gggtcgtgga gagcttcgtt cactacttca acaaattcag ctttgggata acggttggcc
     1441 acagcctgaa accactcgcg aatttcttcg agctgttcac tggccgggag gttttcaatc
     1501 caggcgggct gctgattacc ccacaccagc acatgatgac ggtatggaaa gttgttctta
     1561 agtgcaaaat tgtatgcctc gtcgagttgc ccccacaaca taacatcgcg cgaaggctcg
     1621 gccgatcccc atttccctgc gttttcagga accacctgat tccagtattt ttcaaaattg
     1681 tcttttgtat gcgaagcata tgcacatccc aaatatttcg acagcccgta tgccaagggc
     1741 tgatcagcaa gatcaatttc tggcaagtca acagaacctt cttccccgtt atcgagattc
     1801 tttacggtat aatacaaccc ggcgggtgca aacacaaact tatcgatctc aagcccgtct
     1861 tccctgcccc caatttgaaa agtgaacggt ttcccagctt catcggcaga gaaggtgatt
     1921 ttggtttcag ccaaagaaaa tgctgaaagg ttaacccatt tccaaaccag atttccggca
     1981 ccaccatctt catatactac gcttttctga ttatcgtatc ctgtaaggct caactgatta
     2041 acagtaatcc aatcattccc ttcagtctcc gattttgttc caaaaccatt tccgtagaaa
     2101 aagctgtcgt cgtttgcccc atcggcacca ac
//
LOCUS       DAOPUW010000336         2124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_55945_length_2124_cov_3.158047, whole genome shotgun sequence.
ACCESSION   DAOPUW010000336 DAOPUW010000000
VERSION     DAOPUW010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55945_length_2124_cov_3.158047"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PKJ43_06285"
     CDS             <1..592
                     /locus_tag="PKJ43_06285"
                     /inference="COORDINATES: protein motif:HMM:NF037085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HNZ72211.1"
                     /translation="DAGSYSISTQGGSAENYSFVYQNGSLTVGKATITVTALDASMKR
                     GQQMPVFEMGYLGFKNGDGIADIDHLPEVATSATNDSPAGTYLLTPDGARDNNYSFVY
                     VNGSLTIEPGVGVLQLDRVSVSVFPNPFSDGLTIETTDANAGHFSLCNLAGQVLRQGE
                     LCDGKNFLHLEDLRNGVYLVNIVAGNKTQTLRVVKQ"
     gene            792..1244
                     /gene="dtd"
                     /locus_tag="PKJ43_06290"
     CDS             792..1244
                     /gene="dtd"
                     /locus_tag="PKJ43_06290"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNZ72212.1"
                     /translation="MRVVIQKVNNASVTVDGELISTIGQGLLVFVGIEDADKHEDADW
                     LAKKIVQLRIFPDENEVMNRSVIDENGEILIVSQFTLHAMTKKGNRPSYIRASKPTYA
                     IPMYEFFCEAVSKELGKPVARGIFGADMKVNLLNDGPVTIWVDSKNKE"
     gene            1247..>2124
                     /locus_tag="PKJ43_06295"
     CDS             1247..>2124
                     /locus_tag="PKJ43_06295"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNZ72213.1"
                     /translation="MGGRKHFHTFDALRFFAFMKVFLLHLPITAFPVFSFLKGGGGIG
                     VSFFFVLSGFLITYIILEEKSNTGTINLKNFFVRRILRIWPLFYLMILIAFLTPYLLS
                     FFGISASPDGYQPNWLISCLFLENYNIMFSHSEPNVSPLGVMWSLCVEEHFYIIWGLA
                     MFFIKMKKIPVLLIAAIITGSIARGIYLANTISDADVFSNIDYFAWGAIPAYLLVTNA
                     ERFVNATEKIHVSYKIFFAVLVVCYVILSPNIDYNYKSNIEPLLFGAMFSALLSIIIP
                     ANSPLKIADKNILSYLG"
BASE COUNT          610 a          435 c          447 g          632 t
ORIGIN      
        1 cgatgctggc agttactcaa ttagcactca gggcggcagt gccgaaaact acagctttgt
       61 ataccaaaac ggctcgctta ccgtgggcaa ggctacaatt accgttactg cccttgatgc
      121 ttcgatgaag agggggcagc aaatgccggt cttcgaaatg ggttacctgg gattcaaaaa
      181 cggggacgga attgccgata tcgaccattt gcctgaggtt gccacaagcg caacgaacga
      241 cagccctgcc ggtacttacc tgctaacccc tgacggtgcc cgggacaaca actactcgtt
      301 tgtgtacgta aacggttcgc tcactattga accgggcgtg ggggttttgc aattggatag
      361 ggtcagcgtt tcggttttcc caaacccttt ctccgatgga ctgactattg aaacaaccga
      421 tgctaacgca ggacatttct cgttgtgtaa cctcgccgga caggttctga ggcagggtga
      481 gctctgcgat gggaaaaatt tcctgcatct ggaagatctg aggaacgggg tttacctggt
      541 gaacattgtt gccgggaaca aaacccaaac gttacgggtt gtaaaacagt aacataaaat
      601 ataactaatt atttttaaag aggctaccca aaaatggtgg cctcttcttt ttggtgaaga
      661 aattgcagga gaaaaataaa caaattgata attagaatat tatatctaga ttttcaatct
      721 tacttaaaaa atggataaaa aatatcactt ttgtattctt cgcgcatgaa atcaacttaa
      781 aaactaaaac aatgcgggta gtcattcaaa aagtaaataa tgcatcggtt accgtcgacg
      841 gggaacttat ttcaacaata ggtcagggct tgttggtttt tgtgggtatt gaagatgccg
      901 acaaacatga ggatgctgat tggctggcaa aaaaaattgt acaactcaga atattccccg
      961 acgaaaacga agtaatgaac cggtcagtca ttgatgaaaa tggcgaaatt ctaatcgtca
     1021 gtcagtttac gcttcatgct atgacaaaga aaggaaaccg gccgtcgtac atacgggcct
     1081 caaaaccaac atacgccatc ccaatgtatg agtttttctg cgaagctgtt tcgaaagaac
     1141 ttggaaaacc tgttgcccga ggtatttttg gtgccgacat gaaggttaat ttactgaacg
     1201 atggcccggt aactatctgg gttgactcaa aaaacaaaga atagcaatgg gaggccgcaa
     1261 acattttcac accttcgatg cattgcgttt ttttgcgttc atgaaggttt ttctgcttca
     1321 tcttccaatt acagctttcc cggtattttc atttctaaaa ggaggagggg gaattggggt
     1381 ctcgttcttt tttgtgctca gcgggtttct tatcacatat attattcttg aagaaaaatc
     1441 aaatactgga actataaacc tgaaaaactt ttttgttcgc cgtattttac gaatatggcc
     1501 acttttctac cttatgatcc ttattgcctt tttaactccc tatctgttgt catttttcgg
     1561 aattagtgcc tcacccgatg gataccaacc taactggctg atatcatgtc ttttcctcga
     1621 aaactacaat ataatgtttt ctcactcgga accaaatgtt tcgccacttg gggttatgtg
     1681 gtcactttgt gttgaggaac acttttacat tatttggggc cttgctatgt tttttatcaa
     1741 gatgaaaaaa atccctgttc ttttaatcgc cgcaataatt acaggcagca ttgcacgggg
     1801 catatacctc gccaatacta tttctgatgc cgacgtgttt tcgaatattg attattttgc
     1861 atggggggca attccggcat atttgctggt cacaaatgct gaaagattcg ttaacgcaac
     1921 tgaaaaaatt catgtatcct acaaaatctt tttcgcagtt cttgttgttt gctacgtaat
     1981 tctttctcca aatattgatt ataattacaa aagcaatatc gaaccgctac tctttggagc
     2041 tatgttcagc gcattgctgt ccataataat cccagcaaac agtcctttaa aaatagctga
     2101 caaaaacatt ttaagttatt tggg
//
LOCUS       DAOPUW010000337         2108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_56550_length_2108_cov_3.712129, whole genome shotgun sequence.
ACCESSION   DAOPUW010000337 DAOPUW010000000
VERSION     DAOPUW010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56550_length_2108_cov_3.712129"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..430)
                     /locus_tag="PKJ43_06300"
     CDS             complement(35..430)
                     /locus_tag="PKJ43_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNZ72214.1"
                     /translation="MRKILLILILTVYGQALFAQSGQKWLNYRGNNQMQGITTITFPA
                     KVKLKWSYDADGIFKSAPVISDGKIVVGSTSGELLCIDMNGKLLWNFKTENAFEAPAL
                     IHEGVVYAGNLSGMLYAIDLNTGKKKGLP"
     gene            611..1666
                     /gene="ribD"
                     /locus_tag="PKJ43_06305"
     CDS             611..1666
                     /gene="ribD"
                     /locus_tag="PKJ43_06305"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNZ72215.1"
                     /translation="MQVDHSKYMSRCLELARLAEGHTAPNPMVGSVIVHDGKIIGEGF
                     HQKAGMPHAEVNAIRSVKDRSLLSQSTLYVNLEPCSHHGKTPPCSDLIIDERIPKVVV
                     GTVDPNPMVAGRGIARLRDAGCEVFVGVLEQECLWLNRRFFTVHQKGRPYIILKWAQT
                     IDGFVDTNRNGHELGVPNWITNEIARVAVHRQRSTENAILIGTTTALRDNPSLTLRDW
                     YGNKPLRLIFDMNSRLPSSLRVFTGEAPTATIVKAAYTDGRKTNHILISQDNVLDNLL
                     QWLKQNNVQSVVVEGGPYTLQQFISSRTWDEAHVYVGSGCFGEGIKAPVIEGKPRFEE
                     FFGSSRLSVFINGDKIE"
     gene            1741..1812
                     /locus_tag="PKJ43_06310"
     tRNA            1741..1812
                     /locus_tag="PKJ43_06310"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1774..1776,aa:Arg,seq:cct)
     gene            complement(1922..>2108)
                     /locus_tag="PKJ43_06315"
     CDS             complement(1922..>2108)
                     /locus_tag="PKJ43_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72216.1"
                     /translation="PDGFLPENIKMELNKKGETMNAVMINKGNSITYETIYRDEKLVR
                     YVVVFNELGGIIEERVL"
BASE COUNT          591 a          424 c          433 g          660 t
ORIGIN      
        1 aaaaagaaat gggaatataa aacagacaac cagattatgg gagccccttc tttttaccgg
       61 tattcaggtc gatggcataa agcattcccg aaagattccc cgcatacaca actccctcat
      121 ggataagtgc cggtgcttca aatgcatttt cggttttgaa attccatagt agcttcccgt
      181 tcatgtcaat acaaagtaat tctccacttg ttgacccaac cactattttt ccgtcactaa
      241 tgacaggcgc tgacttaaaa ataccatcgg catcgtaaga ccattttaat ttaacttttg
      301 cgggaaaggt aatcgttgta attccctgca tttgattatt gccccgatag ttgagccatt
      361 tttgtcccga ttgagcaaac aaagcctggc catacactgt aagaatcagt atcagaagta
      421 tttttctcat gtattctgtg caaatctttc tttcaaaaat aggcatttcg ttttctcttt
      481 caatttcttt ttgggttaaa gtcgtgttaa acctcaaatc gttcaactgt tttatagact
      541 tactttaacc attttgtgta tttgaaggtt caagtgcaaa cacttttccg ataaatttta
      601 aatttgtgcc atgcaagttg atcattcaaa atacatgtcg cgttgccttg agttggctcg
      661 ccttgccgaa gggcatactg caccaaaccc tatggttgga agtgttatag tgcacgatgg
      721 caaaattatt ggcgaaggat ttcatcagaa agctggtatg ccacatgccg aggtgaatgc
      781 tatccgttcg gtaaaagatc gtagtttgct ttctcaatca acgctgtatg ttaatcttga
      841 accttgttct catcatggca aaacgcctcc atgttccgat cttataattg atgaacgaat
      901 tccaaaggtt gtggttggca ccgtcgatcc aaatcccatg gttgctggaa ggggtattgc
      961 caggttgagg gatgccggat gcgaagtatt tgttggggtt ttggagcagg aatgtttgtg
     1021 gctaaaccgt cgttttttca ctgttcatca gaaagggagg ccatatataa tccttaagtg
     1081 ggctcaaaca attgatggtt ttgtcgatac caatagaaac ggacacgaac tgggtgtccc
     1141 caattggatt accaacgaaa ttgcgcgtgt tgcggttcat cgtcagcggt caaccgaaaa
     1201 tgcaattctt ataggaacta ctacagcact acgcgataat ccttcgctta cccttcgcga
     1261 ttggtatggc aataagccac ttcggcttat tttcgatatg aatagccgcc tcccttcttc
     1321 gttgcgtgtt tttaccggag aagctccaac ggcaacaatc gtcaaagcgg cttatactga
     1381 cggccgcaag actaaccata tacttataag tcaagataat gtccttgata acttattaca
     1441 atggcttaag caaaacaatg ttcagtctgt agttgtagaa ggcggcccat acacgctcca
     1501 gcaattcatt agctcgagga catgggatga ggctcatgta tatgtcggat caggttgttt
     1561 tggcgaagga attaaggctc ctgtaattga agggaaacca cgttttgaag aattttttgg
     1621 gagtagtcgt ctgtcggttt ttatcaacgg agataagata gaatagtttg cccgaatttt
     1681 tgcacagaaa gcgtttttgt atatttttgc acacgcaaag tgaattttgc tttgcacaat
     1741 ggtctcatag ttcaacggat agaacgacag tttcctaaac tgtaaatctg ggttcgattc
     1801 ccggtgggac tacaaagctc tggtttttaa aaaactaggg cttttgtttc tcttattgga
     1861 taatgcaaaa aaaaagctgc cagaaaaacc atagcagctt atgaatattt agattgatct
     1921 attacaatac ccgttcttct ataattccac caagttcatt aaaaacaaca acatagcgaa
     1981 ccaatttttc atcgcggtaa atggtttcat aggtaataga gtttcctttg ttgatcataa
     2041 ccgcattcat ggtttcccct tttttattga gttccatttt tatgttttca ggaagaaaac
     2101 catcggga
//
LOCUS       DAOPUW010000338         2106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_56642_length_2106_cov_2.794734, whole genome shotgun sequence.
ACCESSION   DAOPUW010000338 DAOPUW010000000
VERSION     DAOPUW010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56642_length_2106_cov_2.794734"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PKJ43_06320"
     CDS             <1..613
                     /locus_tag="PKJ43_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNZ72217.1"
                     /translation="ARIVKEQIPEFNSYLRRGKIVARPAINPAVSVLKADGAMSSVVV
                     PSADTMNYSRLEEFMNEEILKTRKGGENGTMQNKNTLAKIPWPFRNWFFYVFKTITIT
                     WGISFPGLGVSANSFGSFMITNIGSLGLDYGFPALLPTANYSFVYVMGGIQKKPVVIN
                     DEIVIRRMMSGSIVFDHRIADAAQGAKLFKGLKYAVKHPEEFL"
     gene            complement(620..1213)
                     /locus_tag="PKJ43_06325"
     CDS             complement(620..1213)
                     /locus_tag="PKJ43_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72218.1"
                     /translation="MALRICTAIIFFTLILSSFSIAKAEEPREHFRGGMFPHAGFLYN
                     NDSDTKIQGFCVGIGGKIVFPVSNHLRLGTEGYVTSYNYKNNEGFYKLGWGGLLFEYV
                     PKISRLMPVLGVAVGGGGIKDLYPVSGNYKDNEYDVAIYKKYSTMVIAPQVSLEYKLT
                     SHINLATKIDYLLYPGNEFTKYIAHGPRVYLGVLFSR"
     gene            complement(1316..>2106)
                     /locus_tag="PKJ43_06330"
     CDS             complement(1316..>2106)
                     /locus_tag="PKJ43_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ72219.1"
                     /translation="MDLSLEAEAFGAEVIITDDEVPAIKGPVVTEYTEIVGLPVPEPS
                     TQRINAFVEAAELLVGQDFGKPVFAGCIGPYSLAGRLLDLTEILTGIFIYPDEVHQLL
                     EKTTQFLVSYLERLKATGVNGVIMAEPAAGLLGEEQCNEFSSNYIRKIVEKVQDDTFW
                     IILHNCGNTASLVDSMVSTGAKAYHFGNAVDMSAILPLVPEEYMVMGNLNPVGVIKLK
                     SPAQITAEVALLRQKTCQYKNFVLSSGCDIPPGTSLENINALFC"
BASE COUNT          623 a          483 c          429 g          571 t
ORIGIN      
        1 tgcgcgaata gtaaaagagc aaattcccga gttcaattca taccttcgca ggggaaaaat
       61 tgtagcccgt ccagcgataa atcccgcagt gagcgttctt aaagccgacg gagcgatgag
      121 ttctgtggtg gttccctcag ccgacacgat gaattacagc cgcttagaag agtttatgaa
      181 tgaagaaatt ctgaagaccc gcaaaggggg cgaaaacggg accatgcaaa ataaaaatac
      241 actggctaaa attccctggc cttttcgaaa ctggtttttc tatgttttta aaacaattac
      301 cataacctgg ggcatttcgt ttcccggact tggcgtatcg gccaatagtt ttggttcgtt
      361 catgattacc aatattggca gccttggcct cgattacgga ttcccggcct tacttcccac
      421 ggcaaattat tctttcgtgt atgttatggg tggaattcaa aagaaaccgg ttgtaattaa
      481 tgacgaaatt gtaatccgcc gcatgatgtc gggttcaatt gtgtttgatc accgcattgc
      541 tgacgccgcc cagggagcaa agcttttcaa aggcttaaaa tacgcagtga aacaccccga
      601 agaatttttg taggattcat tatcgggaaa aaagcacacc aagataaacc cttggcccat
      661 gagctatgta cttggtgaat tcattgcccg gatataaaag ataatcgatt tttgtagcca
      721 ggtttatgtg cgaagtaagc ttatattcaa ggctgacttg cggtgcaatt accatggtgg
      781 aatacttttt atagatggcc acatcatact cattatcttt ataattgccc gaaaccggat
      841 acaaatcttt tatccctccg ccacctactg caacacctaa aactggcatc aaccgtgata
      901 tctttggaac atattcgaat aacaaacctc cccaacccag cttatagaaa ccttcgttgt
      961 ttttgtagtt atacgaggta acgtaccctt cggtacccaa ccttaaatgg ttgctaactg
     1021 gaaaaactat tttaccgcca ataccaacac aaaaaccttg tattttggtg tcgctgtcgt
     1081 tgttataaag aaaacctgca tgcgggaaca taccccccct gaagtgttcg cgaggttctt
     1141 cggccttcgc aattgaaaac gaggataata taagagtaaa aaaaataatt gccgtacaga
     1201 ttcttaatgc cataaatatt gttttccgca aacatatcat tttaatcgaa cctttttgca
     1261 ttggagatag cgaccagcaa aaacggtaat atttcattta cacaaaggtt acctatcagc
     1321 agaacaaagc attgatgttt tcgagcgaag tacctggtgg aatgtcgcag cccgacgaaa
     1381 gcacaaaatt cttgtactga caggtttttt gccgcaacag ggcaacttcg gcagttattt
     1441 gtgccgggct tttcagttta ataaccccaa ccggattaag atttcccata accatatatt
     1501 cttcaggcac taagggcaaa attgcactca tatcgacggc attcccaaaa tgataggctt
     1561 tagcgcccgt gctcaccatc gaatcaacca ggctagctgt attcccgcag ttatgaagta
     1621 taatccaaaa agtatcgtct tgtactttct ccacaatttt acgaatgtaa ttggaagaga
     1681 attcattgca ttgttcttct ccaagtaagc cggcagccgg ttcagccata attacgccat
     1741 tcacgccggt ggctttcaac ctttcgaggt atgaaaccag aaattgggta gttttctcca
     1801 acaattgatg cacttcgtcg ggataaatga agattccggt aagaatttca gttaaatcga
     1861 gcaaccgccc ggccagtgaa tatggaccaa tacacccggc aaaaacaggc tttccaaagt
     1921 cctgcccgac aagcaattca gcggcctcaa caaaagcatt aatgcgttgc gtggaaggct
     1981 cgggaactgg taagccgaca atttcggtat actcagttac cacaggacct ttaatagcag
     2041 gaacctcatc gtctgttata atcacttctg caccaaaggc ttccgcttcg agcgaaagat
     2101 ccatgc
//
LOCUS       DAOPUW010000339         2098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_56956_length_2098_cov_2.168380, whole genome shotgun sequence.
ACCESSION   DAOPUW010000339 DAOPUW010000000
VERSION     DAOPUW010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56956_length_2098_cov_2.168380"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PKJ43_06335"
     CDS             complement(<1..476)
                     /locus_tag="PKJ43_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72220.1"
                     /translation="MRIFFATIVICFLFSSCQSGQRSDAQYSPKTIGLKLVQNLNERS
                     SMMMYRTDFLNTFHYAEACAALGAIRVAGSIKDSVLLRGIEDRYQPILTGFDTLPYNH
                     VDANVLGVIPLALYQCYNDEHFMNMGLSMADKQWNMPRPDGLTDQTRFWIDDVYMV"
     gene            complement(522..1001)
                     /locus_tag="PKJ43_06340"
     CDS             complement(522..1001)
                     /locus_tag="PKJ43_06340"
                     /inference="COORDINATES: protein motif:HMM:NF024093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNZ72221.1"
                     /translation="MKNKPRFIGPLLVVEDMERARTFYRNVLELEVIEDYGENISFEG
                     NFSLHLRSHFQQLIGGLDVKTGGNDGEIYFEFDDLESLDKRLKSMQVEYVHPLREQPW
                     RQRVVRFYDPDHHIIEVGESFPCIFKRLEKDGLPYDEIARVTLMPVDYVESCLKKEM"
     gene            complement(991..1419)
                     /locus_tag="PKJ43_06345"
     CDS             complement(991..1419)
                     /locus_tag="PKJ43_06345"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNZ72222.1"
                     /translation="MKYNKLTPNFQVKNVAETVRFYTEHLGFLLVAAVDSASNEVDNE
                     LKEGKVYAFAIMVRDGVEIMLQESESFVHDVEISSIDTIGASVSFYVEIEGIDELHNS
                     LKGIASEITSLKTTWYGAKEFYVKDNNNYILGFSEKQHEK"
     gene            complement(1447..>2098)
                     /locus_tag="PKJ43_06350"
     CDS             complement(1447..>2098)
                     /locus_tag="PKJ43_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNZ72223.1"
                     /translation="VLPDGYFDLIFNRLDNKLDRIFLTGTRTIPVDVPFTKNTSHFGI
                     RFKLIASEYIFRESIERIIDTSTNLPLHYWNVSNETFEDLETFSIEMGRIIERFIPDY
                     TEIDRRKFSLMGCLYDNHGDVSVAELSERVGWSSRQINRWFNNQFGLPLKTFANILKC
                     HHAYRQIAQGRFIPESAYYDQAHFIKEIKRYTGYTPKELFELKNDRFLQLSTHDNG"
BASE COUNT          664 a          455 c          369 g          610 t
ORIGIN      
        1 cataccatat atacatcatc gatccagaaa cgtgtttgat cagtcaaccc gtcaggccga
       61 ggcatattcc actgcttatc agccattgac aaacccatat tcatgaaatg ctcatcgttg
      121 taacattgat aaagcgctaa tggtattaca cctaaaacat tagcatcgac atgattatat
      181 ggcaatgtat caaaacccgt caaaattggc tgatatcggt cttctatccc tcgtagtaat
      241 accgagtctt tgattgagcc ggctacacga atggctccca aggcagcaca tgcttcagca
      301 taatgaaacg tattgagaaa atcggtacga tacatcatca ttgaagatcg ttcgtttagg
      361 ttttgcacca atttcaagcc tatcgtcttt ggcgaatatt gggcatcact tcgttgaccc
      421 gattggcatg aagaaaataa aaaacaaatg acaattgtgg caaagaatat tctcataatc
      481 atatcgcacc attagttaat atgtcataaa atccttttcg tctacatttc ttttttcagg
      541 caggattcaa cataatcgac aggcattagg gtaacgcggg caatttcgtc gtatggaagc
      601 ccatcttttt caagacgttt aaagatgcaa gggaaagact cccctacctc aataatatgg
      661 tggtcgggat cataaaacct cacaacacgc tgacgccaag gctgctcgcg caaagggtgg
      721 acatattcaa cctgcataga ctttaagcgc ttatcgagcg attcaagatc atcaaactcg
      781 aaatatatct ctccgtcatt accgccggtt ttaacatcca agccacctat tagttgctgg
      841 aaatgtgatc gtaagtgaag tgaaaaattc ccttcgaaag aaatattttc accataatcc
      901 tcaataactt caagttccag cacgttacga tagaacgtac gtgcccgctc catgtcttca
      961 accacaagca gcggaccaat gaatcgcggt ttatttttca tgttgttttt ctgaaaagcc
     1021 gagtatataa ttattattgt cttttacgta aaactctttg gccccatacc aagtggtttt
     1081 taacgaagta atctcagagg caataccctt gagactattg tgtagttcat caattccttc
     1141 tatttcaaca taaaacgaaa ccgacgcgcc aatagtatcg atggaggaaa tctctacgtc
     1201 gtggacaaag ctttccgatt cctggagcat aatttctacc ccatcacgaa ccattatggc
     1261 aaaggcgtat actttccctt cttttagctc attatcaacc tcattggaag cactatcaac
     1321 tgcggccacc aacaaaaaac ctaaatgctc agtataaaaa cgaaccgttt cggcaacgtt
     1381 tttcacttga aaatttggag ttaacttatt gtatttcata gcataatttt taaaacttcc
     1441 acaaagttaa ccattgtcat gtgtcgataa ttgtaaaaat cggtcatttt tcaattcgaa
     1501 gagttcttta ggcgtgtacc cggtatatcg tttaatctct ttaataaaat gagcctgatc
     1561 gtaataggcg ctttcgggga taaatcttcc ttgggcaatt tgccggtaag catgatggca
     1621 tttcaaaatg ttggcaaaag tttttaaggg caatccaaac tgattgttaa accagcgatt
     1681 aatttgacga ctgctccacc ctacccgttc cgaaagttca gccacactta catcaccatg
     1741 attatcataa agacacccca tcaatgaaaa cttacgccta tctatttcag tataatcagg
     1801 tataaagcgt tcaataattc tacccatttc tattgaaaat gtttcaaggt cttcgaaagt
     1861 ttcattagaa acattccagt aatgaagggg caaattggtc gatgtatcga taatccgctc
     1921 aatcgattca cgaaaaatgt attctgaagc gatcagttta aaacggattc caaaatgcga
     1981 agtattttta gtaaaaggga catcaaccgg aatagtccgg gttccggtaa gaaatattcg
     2041 atcaagctta ttatctaacc tattgaaaat aagatcgaaa tatccatcag gaagcaca
//
LOCUS       DAOPUW010000340         2093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_57128_length_2093_cov_3.743376, whole genome shotgun sequence.
ACCESSION   DAOPUW010000340 DAOPUW010000000
VERSION     DAOPUW010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57128_length_2093_cov_3.743376"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2093)
                     /locus_tag="PKJ43_06355"
     CDS             complement(<1..>2093)
                     /locus_tag="PKJ43_06355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ72224.1"
                     /translation="PLIEQITHQPNQENSLKNNEVNEAIEDKNGDLWLATNNGISQWK
                     RNENQWKHFYANNQDQAQVFLTIHEDNQGRIWAGSYASGLHIIDRETGKELAHYDKSI
                     PGSPIDVNFVYDIFTDSKGEIWICGINSEVVRYSPTENKFTKYSQQPVSMITEYSDSI
                     MLLACSYGLVQLNKNTSAYKILIDKHIVEDILPLNDNIWVATSGEGLLRYNMINNNLE
                     KFTTKNGLPSNYINSLAFSNNYLWLGTESGLCRFDPKQKSAVVFSSIEPLSSSSFNNH
                     AYLHLRDGRLAFGSNNGLIIFNPQTISEKQNSGKIFIQDFSILGRSLRDISSFKLKQP
                     INNIEHFKLKHNQNSLTFEILPVGTIKDAKFSWKIDDSNEWSTPSKQRHITFNNLPHR
                     DYTLKIKMLDNSLSYVVDERTVYFTIAAPFWASWWFLIIVFTTLFILIYSLFWYYINK
                     LKQQHAEEKIRYFTNTAHDIRTSLTLIKAPVDELSHETNLSARGKLFLKLATEQTNRL
                     ANVITQLMDFQKADIGKEKLIMKMTDVVELVKYRTEMVEPLCKTKNIKLIFKTNTNHY
                     ETAIDDIQFEKVIDNLISNAIKYSRENSTVYISLECKNTEWNLVVKDEGIGISKADQK
                     RLFKEFYRGENAVNSKVVGSGVGLLLSKNIVLLHGGNITFNSEENKGSEFLITIPHQK
                     MSDIKAEQTSENERI"
BASE COUNT          542 a          367 c          425 g          759 t
ORIGIN      
        1 tttatccttt cattttcaga agtttgctca gctttgatgt cggacatttt ttgatgcggg
       61 attgttatta aaaattctga acccttattt tcttcactgt tgaaggttat attcccaccg
      121 tgaagcaaaa cgatgttttt tgatagtaaa agaccaacac cagaacctac aacttttgaa
      181 ttaacggcgt tttctccccg gtaaaactct ttgaatagcc gtttctgatc ggctttcgat
      241 attccaattc cttcatcttt tactacaagg ttccattcag tattcttgca ttcaagagaa
      301 atatataccg ttgagttttc tctcgaatat tttattgcat tcgaaatcag gttgtctatt
      361 actttctcaa actgtatatc gtcaatagct gtttcgtagt ggtttgtatt cgttttgaat
      421 atcagtttta tattttttgt tttgcataaa ggttctacca tttcggtcct atattttact
      481 aactctacca catcggtcat tttcattata agcttttctt tcccgatatc ggctttttga
      541 aaatccatta gttgtgtaat aacgttagca agcctgtttg tttgctctgt tgctagtttt
      601 aaaaagagtt taccgcgtgc cgaaagattg gtttcgtggc tgagttcgtc aactggggct
      661 tttattaaag ttaacgaggt gcgaatatcg tgggcagtgt tggtgaagta cctaattttt
      721 tcttcggcat gctgttgttt tagtttgttg atgtaatacc aaaacaatga gtatattagg
      781 atgaagagcg tagtaaagac gataataagg aaccaccacg aagcccagaa cggagctgca
      841 attgtgaaat ataccgttcg ttcatcaacc acatacgaaa gggaattatc gagcatcttg
      901 atctttaggg tgtaatctcg gtgtggaaga ttattgaagg taatatgccg ttgttttgat
      961 ggggtagacc attcattgga atcgtcaatc ttccacgaaa attttgcgtc ttttatggtt
     1021 ccaacgggca gtatttcaaa tgtcagcgaa ttttgattgt gttttaattt aaaatgttcg
     1081 atgttgttga tgggttgctt tagcttgaat gaagatatat ctcgtagtga acggcctaat
     1141 atgctgaaat cttgaatgaa tatcttccct gaattttgct tttcagaaat tgtttgtgga
     1201 ttaaagatta ttaggccgtt attagaacca aaagccagcc ttccgtcacg taaatgaagg
     1261 tacgcgtgat tgttgaacga tgaactggat aagggttcta ttgatgaaaa aacgactgca
     1321 cttttttgct tgggatcgaa tcggcataaa ccactttcgg tacccaacca taaataattg
     1381 ttagaaaaag ccaggctatt tatgtaattt gatggaagcc cgtttttggt agtgaatttt
     1441 tctaagttat tgtttatcat attgtaacgc aatagccctt cgccgcttgt ggctacccat
     1501 atattgtcat ttagaggaag aatgtcttca actatatgtt tgtctataag tattttatat
     1561 gcagaggtat ttttattgag ttgcacaagt ccatacgagc aggccaatag cattatcgag
     1621 tctgagtact cggttatcat tgaaacaggt tgttgagaat acttagtgaa tttattttct
     1681 gtgggagaat atcttacaac ttcgctgttg atcccacaaa tccatatttc gcctttcgag
     1741 tcggtaaaaa tgtcataaac aaagtttacg tcgatcggtg agccggggat gcttttgtca
     1801 taatgggcaa gttctttgcc agtctccctg tcaataatat gtaagcccga ggcatagctt
     1861 ccggcccaga ttcttccctg attatcttca tggatggtca agaaaacctg tgcttgatct
     1921 tgattattcg cgtaaaaatg cttccattgg ttctcatttc ttttccattg gcttatcccg
     1981 ttatttgtcg ctagccagag gtcgccattt ttgtcttcaa tggcttcatt tacttcgttg
     2041 ttcttcagtg aattttcttg gtttggctgg tgggtgattt gctcaataag ggg
//
LOCUS       DAOPUW010000341         2090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_57237_length_2090_cov_3.492875, whole genome shotgun sequence.
ACCESSION   DAOPUW010000341 DAOPUW010000000
VERSION     DAOPUW010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57237_length_2090_cov_3.492875"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1668)
                     /gene="atpA"
                     /locus_tag="PKJ43_06360"
     CDS             complement(85..1668)
                     /gene="atpA"
                     /locus_tag="PKJ43_06360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNZ72225.1"
                     /translation="MADIRPAEISAILKEQLKGFDSLPTMEEVGTVLQVGDGIARIWG
                     LSNVEADEMIEFSNGVTGIALNLEEDNIGAVLLGTSDGISEGSTVKRTRRIASIMAGD
                     GLLGRIINTVGTPIDGKGSIPGDLFEMPLERKAPGVIFRHPVKEPLQTGIKAIDAMIP
                     IGRGQRELIIGDRQTGKTAIAIDTIINQRENFLKGEPVYCIYVAIGQKASTVVSIAHT
                     LEKHGAMDYTIIISATAAEPAALQFYAPYAGAAIGEYFRDTGRHALIVFDDLSKQAVS
                     YREVSLLLRRPPGREAYPGDIFYLHSRLLERAAKIIESDDIARKMNDLPECLIGKVKG
                     GGSLTALPIIETQAGDVAAYIPTNVISITDGQIFLESNLFNAGIKPAINVGISVSRVG
                     GHAQIKAMKQVAGTLKLDQAQYRELEAFSKFGADLDAATRQVLEKGARNTELLKQKQY
                     STLNIAHQIAIIYCGNLNLLSKVPIDKIAQFETEFYEYLDLNCPEVLNELGKGILSDN
                     VCETLTKVANDLSYKYIEE"
     gene            complement(1670..>2090)
                     /gene="atpH"
                     /locus_tag="PKJ43_06365"
     CDS             complement(1670..>2090)
                     /gene="atpH"
                     /locus_tag="PKJ43_06365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNZ72226.1"
                     /translation="YQGFRDSLVGQKMKANEKLGRLNEMFADSFQPLTLRFIELIVNN
                     CRYQYLSDICRNCLDMIRSKEQISIGKIVFAQPVGQELVDELKDKFQKKTKRNIELSS
                     ETDSSLIGGFIFTIDGMQYDASIATRLATIKKQLQKK"
BASE COUNT          549 a          481 c          426 g          634 t
ORIGIN      
        1 ccgaaaccat tttcattgca ctggtaactt gccgtgtgct ttttattgat gcaatgcgaa
       61 gccgtatctc tttaagcttt gccattactc ttcaatatat ttgtaagaca aatcgttggc
      121 cacttttgta agtgtctcgc aaacattatc gcttaaaatt cctttcccca gttcatttaa
      181 tacctcagga caattcaagt cgagatattc ataaaactct gtttcaaatt gggcaatctt
      241 atcaatagga actttactca aaaggtttaa atttccacag tagataatag caatctggtg
      301 cgcaatattt aatgtgctat attgtttctg tttaagcagt tcggtgtttc gtgccccttt
      361 ttcaagaact tgcctggtag ctgcatcgag atcggcccca aacttagaaa aagcttccaa
      421 ttcccgatat tgagcctgat cgagttttaa tgttccagcc acttgtttca tggcttttat
      481 ctgagcatgc cctccaactc gcgatacaga aattcctacg ttgatagctg gttttattcc
      541 tgcattgaac aagttcgatt cgagaaaaat ctgcccatcg gtaattgaaa ttacattggt
      601 gggaatgtat gcagcaacat cgcctgcttg agtttctata ataggaagag cggtaagcga
      661 accacctcct ttgacctttc caatcaaaca ctctggcaaa tcattcattt tcctggcaat
      721 atcatctgat tcgataattt ttgcagcccg ttcgagcagc ctggaatgga ggtagaatat
      781 atcgccagga taagcctcgc gcccgggtgg cctgcgaagc aaaagagaca cttcgcggta
      841 cgaaacggct tgtttcgata agtcgtcgaa aacgattaaa gcgtgacgcc ccgtatcacg
      901 aaaatattca cctatggcag cgcctgcata gggggcgtaa aactggaggg cagcaggctc
      961 ggcagcagtt gcagaaatga ttattgtgta atccatagcg ccatgtttct cgagggtatg
     1021 ggcaatgctc accactgttg atgccttttg accaattgct acgtaaatgc aatatacagg
     1081 ttctcctttg agaaaatttt cccgttgatt aataatagta tcgatggcaa ttgctgtttt
     1141 ccctgtttgc ctgtcgccaa taatgagttc gcgctgacct cgtccgatgg gaatcatggc
     1201 atcgatagct ttaatacctg tttgaagcgg ctctttcact gggtgcctga aaattacccc
     1261 gggagctttt ctctcgagcg gcatttcgaa gaggtcacca gggatagacc ctttcccgtc
     1321 gattggtgta ccaacagtgt tgattatacg accaagcagc ccgtcgccag ccataataga
     1381 agcaattcgc cgtgttcgtt ttacggtact cccttcgcta attccgtctg aagttcccaa
     1441 taaaacagca ccaatgttat cttcttcgag gttgagtgca attccagtta cgccgttgga
     1501 aaactctatc atttcgtcgg cttcaacatt cgaaagcccc cagatacgtg caattccatc
     1561 accaacctga agcacagtac ctacttcttc cattgtgggc agggagtcga aacctttcaa
     1621 ctgctcttta agtatagccg aaatttcggc gggtctaata tctgccatgt tactttttct
     1681 gtaattgttt ttttattgtc gctaaacggg ttgcaatgct ggcatcgtat tgcattccat
     1741 cgatggtaaa aataaagcct cctatgagtg acgaatctgt ttcagaagaa agctcgatat
     1801 tccttttggt ctttttctga aacttatctt ttaattcgtc aaccaactcc tgccctactg
     1861 gctgggcgaa aacaatttta ccaatcgaga tttgttcttt acttcgaatc atgtcgagac
     1921 aattgcggca aatatcagac aggtattgat acctgcaatt gttcacaatg agttcaataa
     1981 accttaatgt aagtggctga aacgaatcag caaacatttc attcagccgg ccaagctttt
     2041 cgttggcctt cattttttgc ccgacaaggg aatcgcgaaa accctggtaa
//
LOCUS       DAOPUW010000342         2086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_57409_length_2086_cov_2.926637, whole genome shotgun sequence.
ACCESSION   DAOPUW010000342 DAOPUW010000000
VERSION     DAOPUW010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57409_length_2086_cov_2.926637"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="PKJ43_06370"
     CDS             <1..940
                     /locus_tag="PKJ43_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNZ72227.1"
                     /translation="GKSDGEVMRFCQSFMTELSKYIGADTDVPAGDIGTGGREIGYMF
                     GQYKRLRNEFVGVLTGKSLEWGGSLIRPEATGYGSVYFAQEMLATRGGNLKGKVCTVS
                     GSGNVAQYTVEKLIQLGAKPVTMSDSSGYIYDPAGIDTEKLAWVMELKNIRRGRISEY
                     AEKFGCEFVAGKTPWGVKCDCAFPSATQNEVNENDAKTLIANGCSLVSEGANMPSTPD
                     AIEVYIKAKILYGPGKAANAGGVATSGLEMTQNSMRLPWSREEVDAKLHQIMINIHKT
                     CVKYGTEADGYINYVKGANIGGFIKVADSMIAQGLV"
     gene            complement(998..>2086)
                     /locus_tag="PKJ43_06375"
     CDS             complement(998..>2086)
                     /locus_tag="PKJ43_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNZ72228.1"
                     /translation="DHTFQQLPSLLPENSLLVFNNTRVIRARLHFTKKTGARIEIFIL
                     DPHQPSDYALNFQQTRECSWKCIVGNLKKWKSGELTHAVSIGNETVAIKASQLENNGD
                     TVLVKFEWENPRFTFAELLDAAGSIPIPPYLNRESETIDQTRYQTVYSKIKGSVAAPT
                     AGLHFTDEVLNTLSTRNISTAELTLHVGAGTFQPVKSETIARHEMHTEHFSVNRALVE
                     KLMSHNGKVIAVGTTSVRTLESIYWLGCKLKEAPKSIADEMFVEQWEPYQNNSDIEKT
                     EALGAIISYMDMNHADHIEAATQIIIVPGYQFKIVEGLITNFHQPKSTLLLLISAYVG
                     NDWQLIYAHALTNEYRFLSYGDSNLYLK"
BASE COUNT          561 a          398 c          490 g          637 t
ORIGIN      
        1 cggaaagagc gacggagaag tcatgcggtt ctgtcaaagc tttatgaccg aactttcaaa
       61 atatattggt gccgatactg acgttcctgc tggcgacata ggtactgggg gtcgcgaaat
      121 tggctatatg tttggccagt acaaaaggct gcgcaacgaa ttcgtaggtg tgttaacagg
      181 aaaaagcctt gaatggggcg gtagcttaat tcgccctgaa gcaactggtt acggttcggt
      241 ttactttgct caggaaatgt tggcaacccg tggtggaaat ttgaaaggta aagtttgtac
      301 tgtttctggc tcagggaacg ttgctcaata tactgttgaa aaacttattc agttgggagc
      361 aaaaccagta acaatgtctg actcatcggg ttacatttac gacccagccg gtatcgatac
      421 cgaaaaattg gcttgggtta tggaacttaa aaatatccgt cgtggacgta tttctgaata
      481 tgctgaaaaa ttcggttgcg aatttgttgc cggaaaaact ccttggggtg tgaaatgcga
      541 ttgcgctttc ccatctgcca ctcaaaatga agtgaacgaa aatgatgcta agacactgat
      601 agccaacggt tgttctctgg tttcagaagg tgcaaatatg ccttcgactc cggatgcaat
      661 tgaagtttac atcaaggcaa aaatacttta cggccctgga aaagcagcca atgcgggtgg
      721 tgttgccact tcaggtctcg aaatgacaca aaactcgatg cgtcttcctt ggtcgcgcga
      781 agaagttgat gcaaaattgc accaaatcat gattaatatt cacaaaactt gcgtgaaata
      841 cggtaccgaa gccgatggtt acatcaacta tgtgaaaggt gccaacattg gcggtttcat
      901 taaagtagct gattcgatga tcgctcaggg tttagtatag taattcatca ttcttcacat
      961 ataaggcgtt ccattattgg ggcgcctttt ttgcttttta tttcaggtaa agattgctgt
     1021 ctccataact taagaagcgg tactcgttag ttaaggcatg agcgtaaatc agttgccagt
     1081 cgttgccaac atacgccgaa atcaatagca ataaagtact tttgggttgg tgaaaattag
     1141 ttatcagtcc ttcaacgatc ttaaattgat aacctggtac aatgatgatt tgtgtggccg
     1201 cttcaatgtg gtctgcatgg ttcatatcca tgtagctgat tatggcgcca agtgcctctg
     1261 ttttttctat atctgaatta ttctgataag gctcccattg ttctacaaac atctcatcgg
     1321 caatggattt tggggcttct tttagtttac agccgagcca ataaatgctt tcaagcgtcc
     1381 tgacagatgt ggttccaact gcaataactt tcccattgtg cgacattagt ttttcaacca
     1441 gcgcgcgatt gactgaaaaa tgttcggtgt gcatttcgtg cctggcaatg gtttctgatt
     1501 ttacaggctg aaatgttcct gcacctacat gaagagtaag ttctgccgtg gaaatattac
     1561 gtgttgaaag ggtattcagt acttcatcgg taaaatgcag gcctgctgtt ggtgcggcaa
     1621 ccgatccttt tatttttgaa tataccgtct ggtagcgggt ttggtcaata gtttccgatt
     1681 cacggtttaa atagggcgga atgggtatac tgccagcggc atcaagtaat tcggcgaaag
     1741 tgaagcgagg gttttcccat tcgaatttta ccaatacagt atccccatta ttttcaagtt
     1801 gagaagcttt tatggcaact gtttcatttc caatggaaac agcatgtgta agttctccgc
     1861 ttttccattt ttttaggttg ccaacaatgc atttccaact gcattctctt gtttgctgaa
     1921 aattaagggc atagtcagat ggctggtgcg ggtcgagaat gaaaatttca atccgtgcgc
     1981 cggttttttt tgtaaaatga agccttgctc tgattacacg ggtattattg aaaaccagta
     2041 aactgttttc aggtaatagt gacggtaatt gttggaaagt atgatc
//
LOCUS       DAOPUW010000343         2080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_57637_length_2080_cov_3.267160, whole genome shotgun sequence.
ACCESSION   DAOPUW010000343 DAOPUW010000000
VERSION     DAOPUW010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57637_length_2080_cov_3.267160"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PKJ43_06380"
     CDS             complement(<1..428)
                     /locus_tag="PKJ43_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72229.1"
                     /translation="MRTTRNKTASVGYLLLLMVALLLFSQCKIYTAYKDILSLHHEEL
                     TSEEKGKPRLEYFNSIPIIHVYGSPYEMGFQYGTLLKSQLNSLSELARELFAEKRIEE
                     FMAIAKAASPNLPPAMFEEIKGMSDASGVDLSDLFALNLVP"
     gene            complement(559..2040)
                     /gene="ilvC"
                     /locus_tag="PKJ43_06385"
     CDS             complement(559..2040)
                     /gene="ilvC"
                     /locus_tag="PKJ43_06385"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF003557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNZ72230.1"
                     /translation="MANYFNSIPLRIQLEQLGKCDFMDNSEFANGVEKLIGKKVVVLG
                     CGAQGLAQGLNMRDSGLDVSYALRKIEIDEKHISYVNATENGFNVGTFEEMIPTADLV
                     LNLTPDKYHTPVVNAAMPLMKKGACLSYSHGFNIVEEGIQIRPDITVVMVAPKSPASE
                     VRAEYLRGFGVPTLIAVHRENDPNGDGFEIAKAYCVATGGHKAGVLHSSFVAEVKSDL
                     MGEQTILCGVLQTASILCFDKMIAKGINPNYASKLVQYGWETITEALKLGGITNMMDR
                     LSNPAKIEAFKLSAELKRIMRPLYEKHMDNIMSGVFSSTMMKDWANGDKDLLTWRAAT
                     AETNFEKTPAGDMKITEQEYFDNGTLMVAFIKAGVELAFEVMTETGIKAESAYYESLH
                     ETPLIANTIARKKLYEMNRVISDTAEYGCYLFDHAARPLLADFMNKIDTDVIGKNFNE
                     GKSNHVDNLELIAINEEIRYHEVEMVGAELRSAMESMKAIITE"
BASE COUNT          558 a          430 c          431 g          661 t
ORIGIN      
        1 ggtaccagat tcaacgcgaa caggtcactc aggtcgactc cggatgcatc ggacatgccc
       61 ttgatttctt caaacattgc tggcggaagg tttgggcttg cagccttagc aatagccata
      121 aattcttcga tccttttttc ggcgaaaagt tcacgtgcca gttctgagag tgaattcaac
      181 tggcttttca acaatgtgcc atattgaaat cccatttcat aagggcttcc gtaaacatgg
      241 ataattggga ttgaattgaa atattcgagc cgtggctttc ctttttcttc cgaagtgagt
      301 tcttcgtgat gaagcgacaa tatatctttg tatgctgtat atattttgca ttggctgaat
      361 agcaataatg caaccattaa caataataga tagccaaccg atgcagtctt atttcgtgta
      421 gtcctcattt atttgcccca cagcgtatta agttgcaaag taatacattt ttcacaaacg
      481 aaaaagaaga ataaaaaaac ctgtttcaag gtaaacatga aacaggtttt aacagttgtt
      541 gacagaattc ttagtatact attcagtaat aattgctttc atcgactcca tggcgctgcg
      601 caattcggca ccaaccattt caacttcgtg gtatcttatt tcttcattta tagcaatcaa
      661 ttcaagattg tcgacatggt tgctcttgcc ttcattgaag tttttaccaa tcacatcggt
      721 atcgattttg ttcataaaat cggccaaaag tgggcgagca gcatggtcga acaggtaaca
      781 accatattca gcagtgtctg aaatcacccg gttcatttcg tatagttttt tgcgggcgat
      841 agtatttgca attagtggag tttcgtgaag tgattcgtaa taagctgatt cggctttgat
      901 tccggtttca gtcataactt cgaaagcgag ttcaacccct gctttgataa aagcaaccat
      961 cagggtaccg ttgtcgaaat attcctgttc ggttattttc atatcgccgg caggggtttt
     1021 ttcgaaattt gtttcagcag tagctgctct ccatgttaga aggtctttat cgccattagc
     1081 ccagtctttc atcattgttg aagaaaatac tccactcatg atgttatcca tatgtttttc
     1141 gtaaagtggg cgcataatgc gtttcagctc agctgataat ttgaaagctt caatttttgc
     1201 aggattgctc agacgatcca tcatgtttgt aatgccacca agtttgagcg cttcggtaat
     1261 cgtttcccat ccgtactgaa cgagttttga tgcataatta ggattgatac ccttggcaat
     1321 catcttgtca aagcaaagga tcgatgcggt ttgaagtact ccgcaaagta tggtttgttc
     1381 gcccattaag tctgatttta cttcagcaac aaacgacgaa tgtaaaacgc ctgctttgtg
     1441 tccgccagtg gcaacgcagt aagctttggc aatttcgaac ccatcgccgt tagggtcgtt
     1501 ttcacggtgt acagcaatga gggttggaac accaaaacca cgcaaatatt cagcacgaac
     1561 ttcagatgcc ggtgattttg gggcaaccat tacaactgta atgtcggggc gaatctgaat
     1621 tccttcctca acaatattga atccatgtga gtatgaaagg catgcgcctt ttttcattaa
     1681 aggcatggct gcatttacta cgggggtgtg atatttatcg ggggtaaggt tgagtacaag
     1741 gtcagctgtt gggatcattt cctcaaatgt accaacgttg aagccgtttt ctgtggcatt
     1801 tacataagaa atgtgctttt cgtcgatctc gattttgcgc aatgcatacg atacatccaa
     1861 accactgtcg cgcatgttaa gcccctgagc taagccttgg gcgccgcaac ctaatacgac
     1921 tactttcttg ccgataagtt tttctacacc gttagcgaat tcagaattgt ccatgaaatc
     1981 gcattttccc aactgttcca attggatacg aagtgggatt gagttgaagt aatttgccat
     2041 tttttattaa cgtttcttaa tctctcaatt aattgatatt
//
LOCUS       DAOPUW010000344         2080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_57649_length_2080_cov_2.553086, whole genome shotgun sequence.
ACCESSION   DAOPUW010000344 DAOPUW010000000
VERSION     DAOPUW010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57649_length_2080_cov_2.553086"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PKJ43_06390"
     CDS             complement(<1..763)
                     /locus_tag="PKJ43_06390"
                     /inference="COORDINATES: protein motif:HMM:NF015272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HNZ72231.1"
                     /translation="MKPVYYSEYLQLDKILEAQALESDKHGRHAHDEMLFIVIHQAYE
                     LWFKQVLFEVDSVIHIFEQDKINDNSPDLQIAVHRLQRVCTILKVLVQQIDIMETMTP
                     MDFLDFRDMLRPASGFQSIQFKMLEALLGLKFEHRHGQSYYTSQLTPADVERVKSLEK
                     RSTLLELVSKWLERMPYFKSENLWQDYRAIYASTLNENEMGNLEQFDKRFIADEREGM
                     TLSKDASRASLFIMLYRHYPLMHLPFELIETLLEVD"
     gene            875..>2080
                     /locus_tag="PKJ43_06395"
     CDS             875..>2080
                     /locus_tag="PKJ43_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNZ72232.1"
                     /translation="MSTAINLEFNKNEDAMKMAISDLKKINEQVKLGGGKKSIERHKE
                     KGKLTARERLELLFDKNKPTLEVGLLAAYDMYPEQGGCPSAGVVTMLGYVKGRLCMAV
                     ANDATVKAGAWFPMTGKKNLRAQEIAMENRLPIIYLVDSAGAFLPMQDEIFPDKENFG
                     RIFRNNAVMSAAGIPQIAAIMGSCVAGGAYLPIMSDEALIVEGTGSVFLAGSFLVKSA
                     IGEEIDNETLGGATTHCEISGVTDYKMKNDQECIEKIRSLIDKYGKPDKAGFDRAEPQ
                     KPAFPVEDIYGIFPEDMAKQYDMLELIKRLVDNSEVDQYKEEYGKSIICCYARIDGWA
                     VGIVANQRKVVKSKKDEMQFGGVIYNDSADKAARFIMNCNQKKIPLVFLQDVTGFMVG
                     SKSEHGGIIK"
BASE COUNT          633 a          375 c          504 g          568 t
ORIGIN      
        1 catctacttc caacaaggtt tcaattaact caaatggcaa gtgcattaat ggataatggc
       61 gatacaacat aatgaatagc gaagcgcgac tggcatcttt cgaaagcgtc atgccttcgc
      121 gttcatcggc aataaagcgt ttatcgaatt gctccaaatt gcccatttcg ttttcgttca
      181 acgtagaggc ataaatggcg cggtaatctt gccacaaatt ttcgcttttg aaatacggca
      241 tgcgctctaa ccatttgctc acgagttcca aaagtgtgct gcgtttttcc aacgatttca
      301 cgcgttctac atcggccgga gtgagttgtg aagtgtaata cgattgacca tggcggtgtt
      361 caaacttcaa tcccaacaat gcttccagca ttttaaattg aatactttga aaacccgatg
      421 ccggccgaag catatctcta aaatccaaga aatccatggg tgtcatggtt tccatgatat
      481 cgatttgttg caccaatact tttagaatgg tgcatacacg ctgcaaacga tgcacggcaa
      541 tttgcaagtc gggcgagtta tcgttgattt tatcttgttc gaaaatatga atcactgaat
      601 cgacttcaaa caatacctgt ttaaaccata attcgtaggc ttggtgaatc acaataaaaa
      661 gcatttcgtc atgtgcatgg cgaccatgtt tatcagactc caatgcttgt gcttctaaaa
      721 ttttatcgag ttgcagatat tcgctgtaat agacaggttt cataatgttt gaattattgg
      781 gctatattac gagtctaaac aagaaataca aataatcttt gtcatgcttt gaaaatttgc
      841 cttttcgtct atttttacca cataaaatca gtacatgtca acggccataa atttagaatt
      901 caacaagaac gaagatgcca tgaaaatggc catttccgat ttgaaaaaaa tcaacgaaca
      961 ggtgaaactg ggcggtggga aaaagtcgat cgaacgtcat aaagaaaaag gaaaactaac
     1021 agctcgcgag cgattggagc tattgttcga taaaaataaa ccaactttgg aagtgggttt
     1081 gttagcggca tacgacatgt atcccgagca aggggggtgc ccaagtgcag gcgtagtaac
     1141 gatgttgggt tatgtgaaag gcagattgtg tatggctgta gcgaacgatg ctacagtgaa
     1201 agcgggagcg tggtttccga tgaccggtaa aaagaattta cgggcacaag aaattgctat
     1261 ggaaaaccgt ttgccgatta tttatttggt agacagtgcc ggtgcgtttt tgccgatgca
     1321 ggacgagatt tttccggata aagaaaactt cggaagaatt ttcagaaata atgcagtgat
     1381 gtcggctgcc ggaattccgc aaatagcggc cattatgggc agttgcgtag caggaggagc
     1441 ttacttgccg attatgagtg atgaggcttt gattgttgaa ggaacaggtt cggtattttt
     1501 ggccggttcg tttttggtga aatcggctat aggcgaggaa atcgataatg aaacattagg
     1561 cggagccact acacactgtg aaatcagtgg ggttactgat tacaaaatga aaaacgatca
     1621 agagtgtatc gaaaaaattc gttctctgat tgataaatac ggcaaaccgg ataaagccgg
     1681 gttcgaccgt gcagagcccc aaaagcctgc attcccggtg gaagatatct atggtatttt
     1741 cccggaagac atggcgaaac aatacgacat gttggagttg attaagcgtt tggtggataa
     1801 cagtgaagtg gatcaataca aagaggaata cggcaagtcg attatatgtt gctatgcgcg
     1861 cattgatggt tgggcagtgg gaattgtggc gaaccagcgt aaagtggtga agagcaaaaa
     1921 agacgaaatg cagtttggcg gggtgattta caacgattcg gctgataagg cggcacgctt
     1981 tattatgaac tgtaatcaga agaaaattcc gttggtattc ttgcaagatg taaccggctt
     2041 tatggtgggt tctaaatcag aacatggagg aattatcaaa
//
LOCUS       DAOPUW010000345         2079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_57677_length_2079_cov_4.079545, whole genome shotgun sequence.
ACCESSION   DAOPUW010000345 DAOPUW010000000
VERSION     DAOPUW010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57677_length_2079_cov_4.079545"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2079)
                     /locus_tag="PKJ43_06400"
     CDS             complement(<1..>2079)
                     /locus_tag="PKJ43_06400"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNZ72233.1"
                     /translation="EVEVELASENDRFKFKKYKRDLKWENEIWVFLRTKGIVLHGNNL
                     VFTKESELFRDEPKYGIINWINQVKEDLEEKGIVVEQSIKETRYYIGKQELDLKLKME
                     DDWFDLFATIRFGEFEIPFIRLKKNILAGKREYLLPNGEVAILPLHWFANYKDLLQLG
                     EEHDGNLRFSKHHYSIINESIGGIDPKIEEKLKQFETEKFDLLTEPTGLKAKLRSYQK
                     EGYSWMFAVGQKKFGACLADDMGLGKTLQTLTLLQKVKELNEHKTKTKKKQESQLSLF
                     SNENEMGAPASLIIVPTSLVHNWNNEIKKFTPKLKIYKHIGTQRKKTSTVGKIIDNYD
                     IIITTYGTLRNDYEELKNYKFNYLILDESQYIKNPQSKSYQSVCELNSEHKLVLTGTP
                     IENSLSDLWAQINFLNRGLLGSYSFFQKEFIQPVEKKNDEEKEKQLRKLISPFILRRT
                     KEEVASDLPALTQQTRYCEMEPDQKKIYEEQKSSIRKALLENIESVGLKKSSFMALQG
                     LTRLRQLANHPALLGHEESASGKFDDVLHDIEELISENHKVLVFSSFVTHLKLFRDEF
                     EKNGWGYCLLTGQTQNREEVIAEFQNDRKKKLFLISLKAGGVGLNLTAADYIFILDPW
                     WNPASENQAISRAHRIGQDKKVFVYRYITQDSIEQKIEKLKERKSLLAEKFITSNSPF
                     DSASTEEIMEL"
BASE COUNT          475 a          428 c          441 g          735 t
ORIGIN      
        1 agctccatga tttcttctgt cgatgcactg tcgaaaggac tgttcgaggt gatgaatttt
       61 tcggcaagaa gcgatttgcg ttcttttaat ttctcaattt tttgttctat cgaatcttgg
      121 gtgatatagc ggtagacaaa tacttttttg tcctggccaa tacgatgtgc ccggctaatg
      181 gcctggtttt ccgatgccgg attccaccac gggtcgagaa tgaaaatata gtcggctgct
      241 gtgaggttta gcccaacgcc acctgccttc agcgaaatga ggaaaagctt cttctttcta
      301 tcattctgaa attcagcaat tacttcttcg cggttttgtg tttgcccggt taacaagcaa
      361 tacccccaac catttttctc aaattcgtcg cgaaagagtt tcaagtgagt aacaaacgat
      421 gagaaaacga gtactttatg gttctccgag atcagctcct cgatgtcgtg cagcacatcg
      481 tcgaatttgc ccgaagcgct ttcttcatgc cccagcaatg ccgggtgatt ggccaattgc
      541 cgtaagcggg taagcccttg taaagccata aacgacgatt tttttagtcc taccgattca
      601 atgttctcga gaagggcttt tcgtatcgat gatttttgct cttcatatat ttttttctga
      661 tcgggttcca tttcgcaata ccgcgtctgt tgtgttagtg ccggtaggtc gctggccact
      721 tcttctttgg tacgcctgag aatgaatggc gaaattaact ttcgaagctg cttttctttt
      781 tcttcgtcgt ttttcttctc gacaggctgg atgaattctt tttgaaaaaa tgaataacta
      841 cctaataaac cgcggtttag gaaatttatc tgagcccaga gatctgatag cgaattttcg
      901 ataggggttc cggtcagtac aagtttatgc tccgaattta gctcgcaaac cgattgatat
      961 gatttcgatt gtgggttctt aatgtactgg ctttcatcga gaatgagata gttgaacttg
     1021 taatttttta attcttcgta atcgtttctc agggtcccgt aagtggtaat aattatgtcg
     1081 tagttatcga ttatttttcc aactgttgat gttttttttc gctgtgtacc tatgtgtttg
     1141 tagatcttta atttaggagt gaattttttg atttcgttat tccagttgtg taccagtgat
     1201 gtggggacaa taattagcga agctggggcg cccatttcgt tttcgttgct gaaaagcgaa
     1261 agctggcttt cttgtttctt ttttgtcttg gttttgtgtt cgttaagttc tttcactttt
     1321 tgcagaagag tcagcgtttg gagggttttc cccagcccca tgtcgtcggc aaggcaggcc
     1381 ccgaattttt tctgtccaac ggcaaacatc catgagtaac cttctttttg atagcttctt
     1441 agtttcgctt taagccctgt tggttcagtc agtaggtcga acttttcggt ctcgaattgt
     1501 tttaacttct cttcaatctt ggggtctatg ccacctatgc tctcgttaat aatgctatag
     1561 tgatgcttgg aaaacctcag gttgccatcg tgttcttcgc caagctgtaa taaatctttg
     1621 taattggcaa accaatgcaa gggcagaatg gccacttcgc cattgggaag caaatactct
     1681 cttttccctg ccaaaatgtt ttttttcagt ctgataaaag gaatctcgaa ttcgccaaaa
     1741 cgaatagttg caaagaggtc aaaccaatcg tcttccatct tgagcttaag gtcgagttct
     1801 tgtttaccaa tgtaatacct tgtttctttt atggattgtt caaccacaat tcccttttct
     1861 tcaaggtctt cttttacctg gtttatccaa ttgatgatgc cgtactttgg ttcgtcgcgg
     1921 aatagctcag actcttttgt gaaaaccagg ttgttgccat gaagtacaat tccttttgtg
     1981 cgtaggaaaa cccatatttc attttcccat ttcaagtcgc gtttgtattt tttaaacttg
     2041 aagcggtcgt tttcagaggc gagttcaact tcaacttcg
//
LOCUS       DAOPUW010000346         2066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_58271_length_2066_cov_2.835405, whole genome shotgun sequence.
ACCESSION   DAOPUW010000346 DAOPUW010000000
VERSION     DAOPUW010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58271_length_2066_cov_2.835405"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PKJ43_06405"
     CDS             <1..670
                     /locus_tag="PKJ43_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HNZ72234.1"
                     /translation="VYFEAWKSGCKGVTVYRDGSRTGVLISDKKKKTEEEPALSVFPT
                     KRPECLEADVVRFQNNREKWIAFIGLIDGRPYEIFTGLADDEEGLLIPRSVNKGYIIK
                     QREGEETRYDFQYVNKRGYKTTMEGLSYKFDPIFWNYAKLISGTLRHGMPIHKIVELV
                     SSLQFLDDSINTWKAGVARALKKYIPDGTRAEGTECGNCGSTNLIYQEGCLTCKDCGH
                     SKCG"
     gene            829..1347
                     /locus_tag="PKJ43_06410"
     CDS             829..1347
                     /locus_tag="PKJ43_06410"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNZ72235.1"
                     /translation="MRTFVILIASILFLNIDAIAQPYYTYPANKANTEQAVMTENESL
                     ADTAKIRLEQEWNRHLIINSDSRVDTLLEIHREENLRKVGLDGFRVQIFQGTKEEAYQ
                     VKGRFISAHENTRAYVSFNAPDFRVRVGDFRTRSEAIKIKQEVKSEYPAAYVVDDFIS
                     FPDLYSEEIIKQ"
     gene            1453..1758
                     /locus_tag="PKJ43_06415"
     CDS             1453..1758
                     /locus_tag="PKJ43_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72236.1"
                     /translation="MYWLNKLYLLIEKQHNRGQDGAGLVSLKIYYTPGKEFLDLYRSA
                     KENPIQNIFEHIRNLYQMVEEKNTEHLSDAQWAKKNLPFSRETYLSHLRYGTFGRNA"
BASE COUNT          735 a          417 c          386 g          528 t
ORIGIN      
        1 agtttatttt gaagcttgga aatcaggctg caaaggagta acggtataca gagatggctc
       61 tcgtactgga gtattaattt ccgacaaaaa gaaaaaaaca gaagaagaac cagccctgtc
      121 ggtattcccg accaagcgtc cagaatgcct cgaggccgat gtggtaaggt ttcagaacaa
      181 ccgtgaaaaa tggattgcat tcattggcct tatcgatgga agaccatacg aaatattcac
      241 cggtcttgcc gacgatgaag aagggctctt aataccccgt tcggtaaaca aaggctacat
      301 tattaaacaa cgtgaaggcg aagaaaccag atacgacttt caatatgtaa acaaacgcgg
      361 ttacaaaacc accatggaag gactatcgta taaattcgac ccgatattct ggaactatgc
      421 aaaacttatt tcgggaacgt tacgacacgg tatgcctatt cacaagattg tagaattagt
      481 atcgagcctt cagttcctcg acgattcaat taacacatgg aaagctggtg ttgccagggc
      541 gctgaaaaaa tacatccctg acggcacacg cgccgaaggc acagaatgcg gcaattgtgg
      601 ttcgacaaac ctaatttacc aggaaggctg tttgacatgt aaagattgtg gccattcgaa
      661 atgtggctga taaaatatcc gtaaaaaaag aaagaggcag tgtcattttg ataccgcctc
      721 tttctttttt caaactatta tctcccaaaa acattaaaat aaaaatctat ctaaaatcaa
      781 ctatctttgt acacaaaatg caaacaaact cttaattttt aactagtaat gagaaccttt
      841 gtaatactta tcgcaagcat cctgtttcta aacatagacg cgattgcaca accatactac
      901 acatatcctg caaacaaagc caataccgaa caagcagtta tgactgaaaa tgaatctctt
      961 gcagacaccg caaaaatcag gctcgaacaa gaatggaacc gtcatttaat aattaattca
     1021 gattcaaggg ttgatacctt attggaaatt catagagaag aaaaccttcg aaaagttggc
     1081 cttgatggat ttcgcgtgca aatcttccaa ggcacaaaag aagaagccta tcaagttaaa
     1141 ggccggttta tttctgccca tgaaaatacc cgtgcatatg tttcgttcaa tgcacccgat
     1201 tttagggttc gcgttggaga cttcagaaca agaagtgaag caataaagat aaaacaggaa
     1261 gtaaaaagcg aatatccggc agcctatgtg gtcgatgatt tcataagttt tcctgatttg
     1321 tattcagaag aaattataaa gcaatagttt gttgggtact aaatgagcaa attaatcaag
     1381 cactaatgtt gagttaccct aattcgactc ctcaagcctc tggaatatta caaagaaaaa
     1441 tacggtacat gaatgtactg gcttaacaag ctttaccttc tcattgagaa acaacacaac
     1501 cgggggcaag atggtgcagg attagtatcg cttaaaattt actatacacc cggaaaagag
     1561 tttctcgacc tataccggtc ggcgaaagaa aacccaattc aaaacatttt cgaacatatt
     1621 cgaaatctat atcaaatggt ggaagagaaa aatacagagc acttatccga cgcccaatgg
     1681 gcaaaaaaga atcttccttt ttccagagaa acttacctga gccacttacg atatggtact
     1741 tttggcagaa acgcttaatc ctgaagattg taataccgaa gttgaattca tttaccagac
     1801 agtcgaaaac tcacataaag ccagccttga tcacaccgga gattggtatt ttacagataa
     1861 cgacccaacc actggagaca ataaagttgt gaatacatcc ttattaattt ctacgaagga
     1921 aaagaaggac gcgcatatta ataaacatac tctctgttaa tccctaaaat tagattatac
     1981 tccaactatc tatatctctg atctttttca aaaaagataa ttaacgatcc cgcaattagt
     2041 ggatcgaaaa ctgcatcaat aaaaca
//
LOCUS       DAOPUW010000347         2058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_58627_length_2058_cov_2.511732, whole genome shotgun sequence.
ACCESSION   DAOPUW010000347 DAOPUW010000000
VERSION     DAOPUW010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58627_length_2058_cov_2.511732"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PKJ43_06420"
     CDS             complement(<1..777)
                     /locus_tag="PKJ43_06420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ72237.1"
                     /translation="MNTYSIIVPVFKSNESLFVLSEQISNMQVQLACTFELILVNDSP
                     WYTETCNALAEIEKIYPFVKVVTLWKNQGQQIALLTGLLFATGKYCITMDDDLQHPVT
                     EIPKLIRSMDEHPQVDAIFACPPYKHKKHSPWRNLASLTMNKIDTFFLKKPKGLKKSA
                     FRIMRKELAIKLTNNYNAMPSVSSLIIFHTHRLMNVEVDHSPRQFGKSNYTLRKMISL
                     ALNDLIHYSSLPLKVLGVVGFAGFLFSIIFILITLIRKLFF"
     gene            850..1989
                     /gene="rffA"
                     /locus_tag="PKJ43_06425"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             850..1989
                     /gene="rffA"
                     /locus_tag="PKJ43_06425"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF008687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HNZ72238.1"
                     /translation="MPDRIPFNQPFHSDDEYVYIKDAFQRNHVSGNGFYTQLCHSFFE
                     EKYGLGRCFLTTSCTDALEMCALLLDIQKGDEIILPSYTFVSTALAFARQGATLRFVD
                     SCSQHPGMDETQIEEKITRQTKAIVVVHYAGIACNMDYIMEIASRYNLVVIEDAAQCI
                     ESDFNGKPLGGIGHLGCFSFHETKNIHCGEGGMLVVNDKKYIARAEKIWEKGTNRAEY
                     FRNQVNKYEWVDTGSSFLPSDILAAMLFAQVQKIDEIQQKRVDSWNRYHSFFSELAKN
                     VSIGIPHIPTYATCNGHLYWLVCASPEERKHLIDFLETRSIHAVFHYQALHSSAYYLS
                     LPKKKETLLYAELFSERLVRLPLYYRLTGKDVDYICDSVRLFFNG"
BASE COUNT          612 a          338 c          434 g          674 t
ORIGIN      
        1 aaagaaaagt tttcgtatca gggtaattag tataaagatg attgaaaaca aaaaaccagc
       61 aaagccgaca acgccaagaa cttttaatgg caacgatgaa taatgtatca aatcatttag
      121 tgccaagctg atcattttac gaagcgtgta attacttttg ccaaactgac ggggcgaatg
      181 atcaacttca acattcatca accggtgggt gtgaaatatg attaaacttg aaactgatgg
      241 cattgcattg tagttatttg tcagctttat tgccaattct tttcgcataa tacgaaaggc
      301 tgatttcttc aatcctttgg gctttttcag aaagaacgta tctattttat tcattgttag
      361 gctggcaagg tttctccatg ggctatgttt tttatgtttg taaggagggc aagcaaagat
      421 ggcatcgact tgaggatgtt cgtccatcga gcggatcagt ttgggtattt cagttactgg
      481 gtgttgaaga tcgtcatcca tagtgatgca atattttccg gtggcgaata aaagtccggt
      541 cagaagggct atctgttgac cttgattttt ccacagtgtg acaactttca caaacggata
      601 tattttttca atttcagcta atgcattgca tgtctcggtg taccaaggac tatcgttaac
      661 caatattaat tcgaaagtgc atgctaattg tacttgcatg ttgctaattt gttctgacag
      721 aacaaagagg ctttcattac ttttgaaaac aggaacaata atagagtatg tgttcataaa
      781 ttgttctttt ctattaaaag atagctaaat gtaatatatt tggttcaaat agtcataaac
      841 gaaaagaaaa tgcccgatag aataccattc aaccaacctt tccatagcga cgacgagtat
      901 gtttatatta aagatgcatt tcaacgaaac catgtttctg ggaacggatt ttatactcag
      961 ttgtgtcata gtttttttga agaaaagtat ggtttaggtc gttgttttct tactacttcg
     1021 tgcaccgatg ccttggaaat gtgtgcatta ctattggata ttcaaaaggg cgatgaaatt
     1081 attcttcctt cttatacttt tgtttcaaca gcgcttgctt ttgcccgaca aggtgcaaca
     1141 ttaaggtttg ttgatagttg ctctcaacat cccgggatgg atgaaaccca aattgaagaa
     1201 aaaataaccc gacaaacaaa agcaattgtg gtggtgcatt atgccgggat tgcctgcaac
     1261 atggattata ttatggaaat tgcttcgcgg tataacctcg tagttattga agatgcggct
     1321 caatgcatcg agagtgattt caacggaaaa ccacttgggg gtattgggca tttggggtgt
     1381 ttctcttttc acgaaacaaa gaacatccat tgtggcgaag ggggaatgtt ggtggtaaac
     1441 gataaaaaat atattgcccg tgccgagaaa atttgggaaa aaggaacaaa ccgagcagaa
     1501 tactttcgga accaagtgaa taagtatgaa tgggtcgata ccggaagttc gttccttccc
     1561 tcagatattt tggccgccat gctttttgct caggttcaga agattgatga aatacaacaa
     1621 aaaagagttg atagttggaa ccgttatcat tcttttttct cagagttggc aaagaacgtt
     1681 tcaatcggaa ttccacacat tcctacttat gctacttgta acggacactt gtattggttg
     1741 gtttgtgctt cgcccgaaga gcgtaagcat ttaatagatt ttcttgaaac acgttcgata
     1801 catgctgttt ttcattatca ggcattgcat tcatcggcct attatttgtc gttaccgaaa
     1861 aagaaagaaa cattgctcta tgccgaatta ttttctgaaa ggctggtaag gttgcctctt
     1921 tattacagat taacaggtaa ggatgttgat tacatttgtg atagtgtgag gctttttttt
     1981 aacggctaat aatatagaaa tctgcaatat agtttgttgt attaattgct tagtatattt
     2041 tttttgtttt gagtgtgt
//
LOCUS       DAOPUW010000348         2058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_58628_length_2058_cov_2.497254, whole genome shotgun sequence.
ACCESSION   DAOPUW010000348 DAOPUW010000000
VERSION     DAOPUW010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58628_length_2058_cov_2.497254"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..651)
                     /locus_tag="PKJ43_06430"
     CDS             complement(181..651)
                     /locus_tag="PKJ43_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72239.1"
                     /translation="MKVSLIAFFAAVLFLGCSKSEETHLNVKLANFSHSECGGFKSES
                     LFADELQEQLIVAHEQKQVYSISHSNVEFNCCLPKGLGIELTLRNDTIYIDEKEIELG
                     LCKCMCPYDLYFELVDLEEGTYVLCLMRGSVVRGTARLGFAKGMMEKIDVSAMN"
     gene            969..>2058
                     /locus_tag="PKJ43_06435"
     CDS             969..>2058
                     /locus_tag="PKJ43_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNZ72240.1"
                     /translation="MKLPFIVLGFLLATVLPACDVLDQEKKVTEITLDQKSATIVEGN
                     NLFGFSFFEKVLSTEDPNINIMISPLSVSQALSMALNGANDNTLSQMKEVLGFDGYST
                     EELNQSNQRIASSLIGHDPKVKMSIANSVWYRKDFSPKKLFIDDNKKYYGAEVSSYDP
                     SKPNEAKKAMNNWVEDNTNGKIEEIIDEVSPESVLFLINAIYFKAEWKSKFKKSETGK
                     APFTLENGTVKEVETMKGEVDLSYYYNNSYTAIELPYGSGKFNMVVFLPEDGYTTADI
                     SGDISNFNFDLLKKSTLSKREIWLPKFEFAYKNELNDELVSMGMSDAFNSSNANFSNL
                     SDIPLFISKVMHKTYIKTDEEGSEAAAGR"
BASE COUNT          692 a          421 c          382 g          563 t
ORIGIN      
        1 cagcctcggt ctcatcgcga caatagcgaa ggcacacccc aaataatttt ggagcaaaaa
       61 gctgataaag cgttgatttt gcttcacgct ttcctgcctt acagtcttct atcagttgcc
      121 cgaggtcttc caacaatcga atgtttatgg gtttaacaca aatgtttcaa caaagttacc
      181 ttaattcatc gctgaaacat caattttttc catcattcct ttagcaaagc ctaaccgggc
      241 tgttcccctg acgaccgagc cacgcattaa acaaagaaca tacgtaccct cttctaaatc
      301 aacaagctca aaatacaagt cgtatgggca catgcatttg caaagtccca attcaatttc
      361 tttttcgtcg atataaatgg tatcgttccg cagggtaagc tctatgccca atcctttggg
      421 cagacaacaa ttgaactcca cgttggagtg acttatacta tatacttgct tctgttcatg
      481 agccactata agttgttcct gaagttcatc ggcaaaaagc gattcacttt taaaaccgcc
      541 gcattctgaa tgagaaaaat tggcaagttt tacattcaaa tgtgtttcct ctgactttga
      601 acatccaaga aaaaggacgg ctgcaaaaaa tgctattagt gatactttca tccgaatata
      661 atttgtttaa aggacgaagg aaccgcatga taaactttca tccttgtttg taactaacga
      721 atcatgcccg tttcataata ctttgaattc gtttttgtat agcagatgaa ggagccgggg
      781 aaaaggttgc gctaaaaaaa ataaaattta acgcaaccta tcgcaacgta taatcatctc
      841 ttggtagatc aacgaaacaa ttggataaaa aatcatttcg ttgcaccgta tgaatattgg
      901 aataagcata aagaaaagaa gatttattgt gcatcaatta atcataacaa attaaaatac
      961 agaatgctat gaaattacca tttatcgttt taggcttttt acttgcaact gttttacctg
     1021 cttgcgatgt tctcgatcag gagaaaaaag taactgaaat tacgctcgac caaaaatcgg
     1081 caacaatcgt agaaggaaac aacctttttg gcttttcgtt tttcgagaaa gtgttatcga
     1141 ccgaagatcc taatataaat attatgattt caccattgag cgttagccag gccttatcta
     1201 tggcactcaa cggggcaaac gacaacaccc tctcgcaaat gaaagaagtg cttggtttcg
     1261 atgggtatag cacagaagag ttaaaccaaa gcaatcaaag aattgccagc tcgcttattg
     1321 gtcacgaccc aaaagtaaag atgtcgatag ccaactcagt atggtaccgc aaagatttta
     1381 gccccaaaaa actatttatc gacgacaaca aaaaatatta cggtgccgaa gtgagcagct
     1441 atgacccatc aaaacccaac gaggcaaaga aagctatgaa caattgggtt gaagataata
     1501 ccaacgggaa aattgaagaa atcatcgacg aagtttctcc cgaaagcgtg ctgttcctta
     1561 ttaacgcgat ttatttcaaa gcggaatgga aaagcaagtt caaaaaaagc gaaactggta
     1621 aagctccttt taccctcgaa aacggaaccg taaaagaggt tgaaaccatg aagggcgagg
     1681 ttgacttgag ctactattac aataatagtt acactgccat agagcttcct tatggctcgg
     1741 gcaaattcaa tatggtagta tttcttcctg aagacggata caccacagcc gatatttcag
     1801 gagatataag caatttcaat tttgatcttc ttaaaaaaag caccttatcg aaacgtgaga
     1861 tttggctgcc aaaatttgaa ttcgcctata agaatgaatt aaacgacgaa ctggtttcta
     1921 tgggaatgtc tgatgccttt aactcatcca acgcaaattt ttcgaatctg tcggacatac
     1981 ctttgttcat ctctaaagta atgcataaaa catacattaa aaccgatgaa gaaggaagcg
     2041 aagctgcggc tggtcggc
//
LOCUS       DAOPUW010000349         2055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_58725_length_2055_cov_4.522500, whole genome shotgun sequence.
ACCESSION   DAOPUW010000349 DAOPUW010000000
VERSION     DAOPUW010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58725_length_2055_cov_4.522500"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PKJ43_06440"
     CDS             <1..396
                     /locus_tag="PKJ43_06440"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNZ72241.1"
                     /translation="IFPVNIIAVTEVSFIIIQRQEVIRLFQKSEQILLNYLSIISSRA
                     QFLSEKLRFHSFKSLKAKIAFYLIKEAGKDNSFKLLHSQNELAELFGVARPSVGRAFL
                     VLQEEGLIDIRYRQVEILDRKRLIEYCNE"
     gene            405..992
                     /locus_tag="PKJ43_06445"
     CDS             405..992
                     /locus_tag="PKJ43_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HNZ72242.1"
                     /translation="MIKLVFATNNQHKLNEVRNMLGENFELVSLEDIGCTEDIAETSK
                     TLEGNASQKAHYVYDNYGLNCFADDTGLEVEALGMAPGVYSARYAGPQRNSIDNMNRL
                     LTELDKIKNRKARFRTVISLIINGNEMLFEGIVDGEILHQPIGQLGFGYDPVFKPEGF
                     STSFAEMDLVLKNSISHRGKAFEKLIHYLKNNYIL"
     gene            989..>2055
                     /locus_tag="PKJ43_06450"
     CDS             989..>2055
                     /locus_tag="PKJ43_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72243.1"
                     /translation="MKHIYLLTIVLLFAFSGWSQIAPGSWDYHLNVSNTQKVVQAGQK
                     IYFMSKGGIYYFNKKDNSIETLDKTDNLSGFDFTAIVYNEATKSLIISYSDATIDVVK
                     EDGSIVPIFDIKRKAITGDKRIWNIYNYNEFCYLACSFGIVVVNLNKMEIKDSYIIGE
                     GGNNENIFDVAVVDDYIFAATNEGIKHAPLNGVNLLDYSTWKLVENPFLPLQPYQNLE
                     VTGNRLWAVHNSEVWHGDKTYSRHAADLWYPEIQDQFIIHDLKLTNGYIVYAAANSFL
                     DGSGNVKYEHYVQLYDEQRKSILKINKYSFSTDKVGIKPLSAIVDNEGIVWIADENYG
                     AVRYENGIYTQLTPQGPFDNNV"
BASE COUNT          666 a          337 c          417 g          635 t
ORIGIN      
        1 atttttccgg tgaatattat tgcagtaact gaagttagtt tcatcataat tcaaagacag
       61 gaagtaataa ggttatttca aaagagcgaa caaatattac tgaactatct ttcgattatt
      121 tcgtcgcgag ctcaatttct ttctgaaaaa cttcgttttc attcgttcaa atcgctaaaa
      181 gcaaaaatag ccttctatct tataaaagaa gcaggtaaag acaattcgtt caagctatta
      241 cattcacaaa acgaattggc ggaattattt ggggtggcaa ggccttcggt gggtcgggca
      301 tttcttgtat tgcaagagga aggcttgatt gacatccgct atcggcaagt agaaattttg
      361 gaccggaaac gtttaattga atattgtaac gaataataaa cccaatgata aaacttgtat
      421 ttgcaactaa taaccagcac aagctcaacg aagtacgaaa tatgttgggc gaaaattttg
      481 aattggtcag tctggaagat atcggttgta ctgaagatat tgccgaaacg agtaaaaccc
      541 ttgaaggaaa tgcttcacaa aaggcacatt atgtatatga taattatgga cttaactgtt
      601 ttgccgacga tactggatta gaagttgaag ctttaggtat ggctcctggg gtttattccg
      661 cacgttacgc tggcccacag cggaattcta tcgataacat gaaccgatta ttaaccgaac
      721 tcgataaaat aaaaaatcga aaagcccgtt ttagaactgt tatttcatta attattaatg
      781 gaaatgaaat gctttttgaa ggtatagtcg atggagaaat cttgcatcag ccaattgggc
      841 aattaggttt tggttacgat ccggttttta aacctgaagg attttcaact tcttttgccg
      901 aaatggattt agtgttaaaa aattcaatta gtcatcgtgg aaaagctttc gaaaaattga
      961 ttcattactt aaagaataac tatattctat gaagcatatt tacttattaa ccattgtttt
     1021 gttgtttgca ttttcaggat ggtcgcaaat agcacccggc tcgtgggatt atcatcttaa
     1081 tgtgtcgaat acccagaaag ttgttcaggc cggacagaaa atttatttca tgagcaaagg
     1141 aggaatctat tattttaaca agaaagataa cagcattgaa accttagaca aaacggataa
     1201 cctttcaggt ttcgacttta cagcaattgt ttataatgaa gctactaaat ctttaataat
     1261 cagctattca gatgcaacaa tcgatgttgt aaaggaagac ggttcgatag ttcctatctt
     1321 cgacattaaa agaaaagcga ttacaggtga caagcgtatc tggaacattt ataattacaa
     1381 cgagttttgt tatttggcat gttctttcgg aattgttgtt gtcaacctta acaaaatgga
     1441 aatcaaagac tcgtatatta taggagaagg tggaaacaac gaaaacatat tcgatgttgc
     1501 cgtggttgac gattatattt ttgctgcaac gaacgaagga ataaaacacg ctccgctgaa
     1561 tggtgtgaat ttgctcgatt attccacctg gaaattggtc gaaaatccgt ttttaccact
     1621 tcaaccatac caaaacctcg aggttacagg caatcggtta tgggctgttc ataacagcga
     1681 agtgtggcac ggcgataaaa cgtatagtcg tcatgcagcc gatttgtggt atcctgaaat
     1741 tcaagatcaa tttataatcc atgatttgaa gcttactaac ggttatatag tttatgcggc
     1801 agcaaattca ttcctcgatg gttctggaaa tgttaagtac gaacactacg ttcaacttta
     1861 tgatgaacag cggaagagta ttcttaaaat taataaatat tctttttcga ccgataaggt
     1921 gggcattaag cctttgtctg ctattgttga taatgagggt attgtgtgga ttgctgacga
     1981 gaattacgga gctgttcgtt atgagaatgg aatttataca cagcttaccc ctcagggacc
     2041 gttcgacaat aacgt
//
LOCUS       DAOPUW010000350         2051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_58929_length_2051_cov_2.785070, whole genome shotgun sequence.
ACCESSION   DAOPUW010000350 DAOPUW010000000
VERSION     DAOPUW010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58929_length_2051_cov_2.785070"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PKJ43_06455"
     CDS             <1..460
                     /locus_tag="PKJ43_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ72244.1"
                     /translation="DESIFTIKEISNNISPHILNNFGLYKALQSFITKIQVSNNIDIM
                     LNSNIGQKRFNYNVEVVFYRIICELITNTLKHASARRITIDLFETDNELILEYIDDGK
                     GFSPEKAEQTGTGMGLSNMRSRVNSLNGRYEAKSKKGMGVCFNISVKVNQ"
     gene            457..1098
                     /locus_tag="PKJ43_06460"
     CDS             457..1098
                     /locus_tag="PKJ43_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010421596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ72245.1"
                     /translation="MKKISVLIVDDHQLFREGLKLLLGVSSIVGEVYQASDGNEFLDF
                     IENMSPDIVLMDINMPIMDGIEATTKALKEHPEMKIIALSMFGDDEYYIKMIEAGAKG
                     FIVKNSDIEVVETAIQQVMEGKTYFASDVMATLVMHLNQRKNEETKNELSERESEILY
                     MICKGLSNQEIADALFLSKRTVDKHRENILAKTQAKNTAGLVLYAIKHNIIQV"
     gene            1289..>2051
                     /locus_tag="PKJ43_06465"
     CDS             1289..>2051
                     /locus_tag="PKJ43_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNZ72246.1"
                     /translation="MKKSPIYFLLILLVSIVSCKSSTKKGNETGEQELKGYISLSGAF
                     ALYPITVQWADEFRKIYPNVRIDISAGGAGKGIADALSQMVDLGMVSREISPAEIEQG
                     AWYIALTKDAVLPTINLANPFLKELAEKGVKSDDLKKVFLTGEIKTWEALLGREGNTP
                     IHLFTRSDACGAAAMWAQFLGKKQEELLGTGVFGDPGAADAVKSDIYALAYNNVNYVY
                     DINTRKKFEKLEVLPIDFNSNNILDPNENIYETLDK"
BASE COUNT          718 a          420 c          407 g          506 t
ORIGIN      
        1 cgacgaatcg attttcacca tcaaagagat atcgaataat atcagcccac acattctcaa
       61 taactttggt ctttataaag cccttcaatc gttcattaca aaaattcagg taagcaacaa
      121 catcgacatc atgcttaata gcaacattgg gcaaaagagg tttaactaca atgtagaggt
      181 ggttttttac cgcataattt gcgagctgat taccaacaca ttaaaacacg cctctgcccg
      241 aaggataacc attgacctgt tcgaaaccga caatgaactc atcctggaat atattgacga
      301 cgggaaagga ttttctcccg aaaaggcaga acagacagga acaggaatgg gtttatcgaa
      361 catgcgttca cgggtaaatt cactaaacgg aaggtatgaa gctaaaagca aaaaaggcat
      421 gggggtttgt ttcaacataa gtgtaaaagt caaccaatga aaaagatatc agtacttata
      481 gtcgatgacc accaattatt tcgcgaaggc cttaagttgc ttctcggcgt tagctcaatt
      541 gttggcgaag tgtatcaggc ctccgacgga aatgaatttc ttgactttat cgaaaatatg
      601 agcccagata tcgtactgat ggatattaac atgccaatta tggacgggat tgaagcaaca
      661 acaaaagcac tgaaagaaca tcccgaaatg aaaataattg ccttgtcgat gttcggcgac
      721 gatgagtatt acataaaaat gattgaagcc ggggcaaaag gattcattgt aaaaaattct
      781 gacattgaag tagttgaaac ggcaattcag caagtaatgg aaggcaaaac ctattttgca
      841 tccgatgtaa tggcgacgtt ggtaatgcac ctcaaccaac gaaaaaacga agaaacgaaa
      901 aatgaacttt cggaacgcga atctgaaatt ctttacatga tttgcaaagg gctatcaaat
      961 caagaaattg ccgatgccct ctttctgagc aaacgaaccg ttgacaaaca ccgtgaaaac
     1021 attctggcaa aaacacaagc taaaaacacg gctggattgg tactttatgc catcaaacac
     1081 aacataatac aagtatagcc ccgatacgtt ttttcaccta ttctttttac gttttttgcc
     1141 tgtttcatgt atccttaaat gcaattagtt ttgtattgac cttgaaaaac aatgcattta
     1201 gtgcgtatat gcaacccacg cacttcatgc ataagttact gaaggacaaa agcaattagc
     1261 aatcgacaat taaccaaaag attacgaaat gaaaaaatca ccaatctact ttttactgat
     1321 cctgttagta agcatagtat cgtgcaaatc atcgactaaa aaaggaaacg aaaccggtga
     1381 acaagaatta aaaggataca tcagcctttc gggggcattt gccctttacc caattacagt
     1441 tcaatgggcc gatgaattca ggaaaatata ccccaatgta agaattgaca tttcagccgg
     1501 aggagcaggc aaaggaatag ccgatgcgtt gtcacaaatg gttgaccttg gtatggtttc
     1561 gcgagaaata tcgccagccg aaattgaaca aggcgcttgg tacattgccc tgaccaaaga
     1621 tgccgtactc ccaaccataa accttgccaa tccgttttta aaggaacttg ctgaaaaagg
     1681 cgtcaaaagc gacgacctga aaaaagtatt cctcaccgga gaaattaaaa catgggaagc
     1741 acttcttggc cgtgaaggta ataccccgat acaccttttt acccgctccg atgcttgtgg
     1801 agcagcagcc atgtgggctc agtttctggg taaaaaacaa gaagaattat tgggaacagg
     1861 cgtttttggc gaccccggag ctgccgacgc agtcaaaagc gacatctacg ctctggcata
     1921 caataatgta aattacgtgt acgatattaa cacaagaaaa aaattcgaaa aacttgaagt
     1981 attgcccatc gactttaaca gcaataatat tttagaccct aacgagaaca tttatgagac
     2041 actcgacaaa a
//
LOCUS       DAOPUW010000351         2039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_59473_length_2039_cov_3.045363, whole genome shotgun sequence.
ACCESSION   DAOPUW010000351 DAOPUW010000000
VERSION     DAOPUW010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59473_length_2039_cov_3.045363"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1792)
                     /locus_tag="PKJ43_06470"
     CDS             complement(56..1792)
                     /locus_tag="PKJ43_06470"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HNZ72247.1"
                     /translation="MNVIFLIVGAIVVFIIAYKIYGTFLARKIFSVDDSKVTPAVSCE
                     DGIDYVPTNPKFLMGQHFSAIAGAGPITGPIIAGILFGWAPALLWIILGSIFIGGVHD
                     MGSLVASIRHKALSITEVVRKNISKRAWILFLMFVWFALVYVIVAFTDITSSAFIGTI
                     TLENGEKVGGGAIATSSLLYLILPLIMGLLLKFTRMSLTWATIIFLPLVGLSIIIGPY
                     IPCNIESAFGLSAISAQKVWNVFLLVYCFIASLIPVWLLLQPRGHLGGYFLYASLITA
                     ALGLMFGGFEISYPAFISSDASGNQTSPMFPMLFITVACGACSGFHAMVSSGTTSKQL
                     KKESDSKVIGYGAMLLEGLVGVVALACLMIISKDNELVKGSPNFIFASGIGSFVELIG
                     IPAAFGISFGLMAFTTFVYDTLDVCTRLARYVLQELTGWRDKYGKAFAAAISIAVPIF
                     LVSMRLTDASGAEVPVWKVFWNIFGASNQLLAALALLGISVWLFKTARFAWAWIITFI
                     PALWMFIMSNWGLLLLIKNNWFTGQEFTPNSNPVPIISLILLLLSIFMAAEVAYIMVK
                     GRNKDSIVEVGE"
BASE COUNT          708 a          462 c          370 g          499 t
ORIGIN      
        1 gaataaaaca taaaattcat tgatttcaca aagtcactat gtccgaaaac acactttact
       61 cacccacctc aacaattgag tctttatttc gtcctttcac cataatataa gcaacttcgg
      121 ctgccataaa tatcgacaat agtaagagaa tgagtgaaat aataggcact gggttggagt
      181 ttggcgtaaa ttcctgacct gtaaaccaat tatttttgat gagaagcaat aatccccagt
      241 tcgacataat aaacatccaa agagccggaa taaacgtaat gatccatgcc caggcgaaac
      301 gagctgtttt gaaaagccat accgaaattc ccaataaagc aagtgctgcg agcaattggt
      361 tcgatgctcc aaaaatgttc cagaaaacct tccatacagg cacttctgcc cccgaagcat
      421 cagtaaggcg catgctcacc agaaaaatcg ggacagcaat tgaaatggct gcggcaaaag
      481 cttttccgta tttatcccgc caacctgtga gttcttgtaa aacatagcgg gccaagcgag
      541 tgcaaacatc cagggtatca tatacaaaag ttgtgaaagc cattagtcca aacgaaatac
      601 caaatgcagc cggaattcca ataagttcaa caaaactgcc aatgccagaa gcaaatataa
      661 aatttggcga tcctttaacc agttcattat ctttcgaaat tatcataagg caagccagag
      721 caacaacccc aacaagccct tcgagcaaca ttgctccata tccaattact ttcgaatcgc
      781 tttctttctt taattgtttc gaagttgttc cactactcac catagcatga aaacccgaac
      841 aagctccgca ggctacagta atgaaaagca ttgggaacat tggcgaagtt tggttccctg
      901 aagcatcgga tgaaataaat gcaggataag aaatttcaaa gcctccaaac attagcccta
      961 acgcagccgt aatgagtgaa gcataaagaa aatatccacc taaatggcct cgtggctgaa
     1021 ggagaagcca aaccggaatc agcgatgcta taaagcagta aaccagaaga aaaacattcc
     1081 acaccttttg tgccgagatt gccgacaggc caaaggccga ctcaatattg caaggaatat
     1141 acggaccgat aataattgat aatccaacta atggaagaaa aataattgtt gcccaggtca
     1201 acgacattct ggtaaatttc aacaataacc ccataattag cggaagaata agataaagta
     1261 aggaagaagt tgcaatggca ccgcctccta ctttttctcc attttcgagc gttattgtcc
     1321 caataaatgc acttgaagtt atgtcggtaa atgcgacaat aacgtacact aatgcaaacc
     1381 aaacgaacat aaggaataaa atccaggccc tttttgaaat attctttcgt actacttcgg
     1441 taatcgacaa tgctttatgc ctaatcgaag caaccaggga ccccatatcg tgaacgccac
     1501 caataaaaat cgatccaagg ataatccata aaagtgcagg agcccagcca aataagattc
     1561 cggcaataat tggtcctgtg attggaccag cgccggcaat agctgaaaaa tgttgcccca
     1621 taagaaactt cggattcgtg ggaacgtagt ctatcccatc ctcacacgaa acagctggtg
     1681 ttactttact atcgtcgact gaaaaaattt tcctggccag aaatgtgcca taaatcttat
     1741 atgcaataat aaaaacgact attgcaccta caattagaaa tataacattc ataatgaatt
     1801 atattatccc ggagccaagc tggtcgaccg ttgtttttat ctcataaaaa taggccaaac
     1861 tttttttaca ccaaacagat ttaacaaaaa ataattctca ttcattgttg tccggtaaaa
     1921 atataaaaac acaaaacaga ttgcttcgct tcgctcgcaa tgacaacatt atctgtttat
     1981 taggtggagg ggaggggttg gttggcggct ttgccgccaa ccaacccctc ccctccaga
//
LOCUS       DAOPUW010000352         2037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_59552_length_2037_cov_4.616549, whole genome shotgun sequence.
ACCESSION   DAOPUW010000352 DAOPUW010000000
VERSION     DAOPUW010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59552_length_2037_cov_4.616549"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PKJ43_06475"
     CDS             complement(<1..466)
                     /locus_tag="PKJ43_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72248.1"
                     /translation="MTAKVRSLLFTAIFIIVVLFSCKPNPLKVDVSGIDVSLSVTRLE
                     KFMFGVNEEEFAKNIETIKRDHLSFFDIYSSYVLNIGTMESPDFNQGLRMFVTDSVFS
                     RVGDSVLLAFKSIDKLEKELTEGFRHFKYYFPNARIPNVYTCVSGFTKSVFIS"
     gene            809..1471
                     /locus_tag="PKJ43_06480"
     CDS             809..1471
                     /locus_tag="PKJ43_06480"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNZ72249.1"
                     /translation="MKKKIILFAALLTLSAAGFAQKMQLGFQASPQVSWLKSTESNIT
                     NENIGLGIRYGLDADIFLFDNTRYALNTGLFITNHSFKARYNLEKPIVVGDKEMPTPV
                     DIKYRVNYIELPLNIKLRSDQFYRFTYYGQFGLTNLINISASGTSSDLKLDGSNVNDA
                     IRFFNLGMIVGGGAEYDVGGNTALNFGIQYTNYFVDATTIKGLSEKSKINSIRLIIGV
                     MF"
     gene            1636..2034
                     /locus_tag="PKJ43_06485"
     CDS             1636..2034
                     /locus_tag="PKJ43_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNZ72250.1"
                     /translation="MFRNDSSNMNSHEPRKSIFNLLSIEEKAEVEQHIQKLEVSKNSI
                     IYHEGEVPTAFMTLEEGKVKIYKEGVGNREQILRMVKPGGFVSYRALIANELHNASAM
                     ALEDSLIYVVDKEYFFSKFIKIYICNRFCF"
BASE COUNT          697 a          384 c          387 g          569 t
ORIGIN      
        1 cggagataaa aacagacttg gtaaagcccg aaacacaagt gtatacatta gggatgcggg
       61 cattgggaaa gtaatactta aaatgccgga agccctcagt cagctctttt tcaagtttat
      121 caatcgattt aaaggccaaa agaaccgagt cgccaacccg tgaaaaaaca gagtcggtta
      181 caaacatgcg caacccttga ttaaaatcgg gcgactccat cgttccaata ttcaatacat
      241 aacttgaata aatgtcgaaa aaagaaagat gatcgcgctt gatcgtctcg atgtttttag
      301 caaattcctc ttcgttaaca ccaaacataa acttctcgag ccgggtaacc gacaacgaaa
      361 catcaatgcc agaaacgtcg acttttaatg ggttgggttt acaagaaaag agaaccacaa
      421 ttatgaaaat tgctgtaaat aaaagacttc gtacttttgc agtcatagtg gttaaatatt
      481 cggttgaaat taaaatgcca ggcacaaatt tacagtattg tattcaattc cggcagaaag
      541 ttttatcgat atgatcaact tacattatac cccgaattcg atactaaaat atggccatac
      601 aacagataaa acggatttcg ttggtggcac attgcctcac ctaaggacaa aatcaacatc
      661 agaaaccaac aattgtgcta tttttgagta taatttgtga gtaacagaaa caatttttcc
      721 ataatattgt atttagataa caataaactg gcaattgtag acatattcca ataatcagaa
      781 atgtcaataa attaatgaat tatctattat gaaaaagaaa attattctct ttgctgcact
      841 cttaaccctt agtgcggcag gttttgccca gaaaatgcag cttggttttc aggctagtcc
      901 gcaagtttcc tggcttaaat caacggaatc gaatataaca aatgaaaaca tagggttggg
      961 gatcaggtac ggtctcgatg ccgacatttt ccttttcgac aatacacgct atgcgctcaa
     1021 caccgggtta ttcataacga accactcgtt caaggcacgc tataaccttg aaaagcccat
     1081 cgtggtggga gacaaagaga tgcccacacc agtagatatt aaataccggg tgaattatat
     1141 tgaactgcca ttgaacatta aattgcgttc cgaccagttt taccgcttca cctattacgg
     1201 acaattcggg ctcacaaacc ttataaacat tagcgcatca ggaacatcaa gcgacttgaa
     1261 acttgatggg tcaaacgtta acgatgccat taggtttttc aacttaggaa tgattgtggg
     1321 gggtggtgcc gaatacgatg tgggcggaaa cactgcctta aactttggca ttcaatacac
     1381 caactacttt gttgatgcca caacaattaa aggcttaagc gaaaaatcga agatcaattc
     1441 aatacggctt atcattggag tgatgtttta aattacttcc gcaataagaa ttattagtta
     1501 caaaaagaca ttttttaccg ttgctgtttc attggtcttg tgaaacatga gtttctgtgc
     1561 aaaaatttgt gcaaaaaaca tgttttacaa cataaaaaat ctaactttgg ccataaaccg
     1621 tacaaaaagt aggatatgtt tcgaaatgat tcatcgaata tgaattctca tgagccgaga
     1681 aaatcaattt tcaatctttt aagtattgaa gaaaaggctg aggttgaaca acatatccag
     1741 aaactagaag tcagcaagaa cagtattatt taccatgaag gggaagtgcc tactgctttt
     1801 atgacccttg aagaaggaaa agtaaaaatt tacaaagaag gggttggcaa tcgcgagcag
     1861 attttacgaa tggtaaaacc cggaggtttt gtcagctaca gggcattaat tgccaacgag
     1921 ctgcacaatg cttcggccat ggcccttgaa gattcgttaa tttacgttgt tgataaagaa
     1981 tactttttct cgaagttcat taaaatctac atctgtaaca ggttctgctt ttaatcc
//
LOCUS       DAOPUW010000353         2037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_59561_length_2037_cov_3.576690, whole genome shotgun sequence.
ACCESSION   DAOPUW010000353 DAOPUW010000000
VERSION     DAOPUW010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59561_length_2037_cov_3.576690"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PKJ43_06490"
     CDS             <1..226
                     /locus_tag="PKJ43_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72251.1"
                     /translation="HSRDGIDWTIFDFNKVYSGFYKTCAFIKVEATPIKLLLLGKPAM
                     DYPFCSSLQKETFGPNVLWITPMMKEFMSH"
     gene            780..917
                     /locus_tag="PKJ43_06495"
     CDS             780..917
                     /locus_tag="PKJ43_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72252.1"
                     /translation="MGRSLAWSMGRNNNSGMTATSFPNSVAVGSSWGPELPVFHLAPD
                     G"
     gene            complement(1117..>2037)
                     /locus_tag="PKJ43_06500"
     CDS             complement(1117..>2037)
                     /locus_tag="PKJ43_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNZ72253.1"
                     /translation="VFLATLEMKKKIGKPMKEILQNIKELESGKIGVSYNKEIASRDD
                     EFGQVYNSVAGLEKSIGQIVKDIKIHADTLAQSSLNIRILSQNFSQGATEQAANLEEL
                     SSTMEEISSNLKTNLKLADETIQVTTYAEEIASHTVSDIGETIGIYNEISSKITSVTE
                     IAFQTNILALNAAVEAARAGEAGRGFTVVASEVRKLAETSRSFADEIIYLSDKSLKKS
                     GHSEKAIGEMMPEIRKAAESVKQIASSNNEQAASINQISISVNQMNNVTQQNAMASEE
                     LTNRAEQLDAQAEALKKSVAFFKFAERTAS"
BASE COUNT          538 a          438 c          429 g          632 t
ORIGIN      
        1 tcactccagg gatggaatcg attggacaat cttcgacttt aataaagtct attccggatt
       61 ttataagacc tgcgctttca ttaaagttga agccactccg atcaaattgc tgttgttggg
      121 aaaaccagcg atggactacc cgttctgttc ttctcttcaa aaggaaacgt ttggtccgaa
      181 cgtgctctgg attactccaa tgatgaagga atttatgtca cactaaaaga aatgccttca
      241 gacatctgtt acgatgcaac ttatgatcga ttcctgttaa catgtgccga aggccggctg
      301 atgataattc cttcctgttc acattgtcag aaagtttacc agataactga tgaaaatctc
      361 cataatcttg ccgtaaatga aagcaagcta attgttgtgg gaaccaataa attctttaaa
      421 atcattaatc ttagtgacct atgagaattc gattttaaac catttgcctt cttcaaaatt
      481 taaggataat ataccaaagg taaagacaat tataaaataa gacacaaaca actaacaatc
      541 taatttattc taaaaaacaa gttttttaga atagcaattc tgcttctttg ctcttttgcc
      601 atgatggctt tcatgccggg cagcgagggt acaaaaaaac cgatttactt aaacacggct
      661 tattctttta aagaaagggc tgccgacctt gtttcgagga tgacacccga agagaaacaa
      721 agtcagttgg gaaataccat gccgccaatt caccgattgt gagttaatgg ccacaatgtg
      781 tggggcgaag ccttgcatgg agtatgggca gaaacaacaa tagtgggatg accgctacat
      841 cgttcccgaa cagtgttgcg gtgggttctt catggggccc cgaactgcct gtgttccacc
      901 ttgctcctga tggataacga aatcccattc gagcgagtcg attttgtaca gtagacatct
      961 cagttggtaa attcccgtac gatgaaaaat aaattttatg caaatcaacg gatacccctt
     1021 gctttcacat gattagcgat agccgggagt ttggtctaac tggttgctga aaagattatt
     1081 gaaattgatt tcaataaatc tgaacctgcg ggggttttaa ctcgctgtcc gttccgcaaa
     1141 cttgaaaaat gccaccgact ttttcagcgc ttcggcctgg gcatcgagtt gttcggctct
     1201 gttggtcaac tcttccgaag ccattgcatt ttgttgggtt acgttgttca tctggtttac
     1261 cgaaatgctg atctgattga tgctggcggc ttgttcatta tttgacgagg caatttgttt
     1321 cacactttcg gcagcttttc tgatttcagg catcatttcc ccgatcgcct tctctgaatg
     1381 tcccgatttt ttcaagcttt tatcagaaag ataaataatt tcatcagcaa aagagcggct
     1441 ggtttctgcc agttttcgca cttcggatgc cactacggta aaaccccgtc cggcttctcc
     1501 tgcacgcgct gcttcaaccg cagcattcaa tgccagaatg ttggtctgaa aggctatttc
     1561 ggtaaccgat gtgattttag aagatatctc gttatagatt ccgatggtct cgccaatatc
     1621 ggaaaccgtg tgcgaggcga tctcttcggc ataggttgtt acctgaatgg tttcgtcggc
     1681 taacttaagg ttcgtcttca ggtttgaaga gatttcttcc atggtcgacg acagctcttc
     1741 aaggtttgca gcctgctcgg ttgctccctg cgaaaaattc tgcgagagga ttcttatatt
     1801 caggctactt tgggccaggg tatcagcatg gattttaatg tcttttacaa tttgtccgat
     1861 actcttttcg aggccggcga cagagttgta aacctgtcca aattcatcat cgcgcgatgc
     1921 aatttctttg ttatatgaaa caccaatttt ccccgattcc aactctttga tgttttggag
     1981 aatctctttc atcggttttc ctattttctt tttcatctcg agggtggcaa gaaaaac
//
LOCUS       DAOPUW010000354         2035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_59656_length_2035_cov_2.933838, whole genome shotgun sequence.
ACCESSION   DAOPUW010000354 DAOPUW010000000
VERSION     DAOPUW010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59656_length_2035_cov_2.933838"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PKJ43_06505"
     CDS             <1..405
                     /locus_tag="PKJ43_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNZ72254.1"
                     /translation="RTGDLGCFDKHGYLYIKGRLKNMIVGASGQNIYPEEIEAIINRF
                     KYVLESVVVQKKGKLVAMVHFNMEELEAKYTNLKDQASQLFEKKIEELSVELQNYVNS
                     QVNKFSRVQLVIVHPEPFEKTATKKIKRFLYY"
     gene            complement(534..794)
                     /locus_tag="PKJ43_06510"
     CDS             complement(534..794)
                     /locus_tag="PKJ43_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HNZ72255.1"
                     /translation="MKEGIHPKNYRLVAFKDMSNEEVVITKSAASTKETIEIDGVTYP
                     LFKLEISSSSHPFYTGKMKLVDTAGRVDKFMNRYAKHMENRK"
     gene            complement(924..>2035)
                     /locus_tag="PKJ43_06515"
     CDS             complement(924..>2035)
                     /locus_tag="PKJ43_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ72256.1"
                     /translation="KIIYKDDPNFTFPLVGMVEENFDPEKNSFFKHGKAIRWIAIDSK
                     EKPLGRIAAFIDFDKAQRFEQPTGNIGFFECTNDQHVANLLFDKAREWLGTHGMQAMD
                     GPANMGENYMNHGLLAWGFMPQGYGMPYNPPYYVELFKNYGFNVYYEQYSYHIDYTKP
                     FPERFWKIAEWVAKKPQFSFKHFDWKDSEKFINDFCTVYEGAWQKHEHFKAIDHYELK
                     SFLNSAKLILDPEFVWFAYHNNEPIALLVMLPDFNQALRYIKNGSLNLLNFLKLLYFL
                     KKGKFTRTRIIIMGIVEKFQKSGIESAIFWHMENDAMLHKPQYKEVEISWAGDFNPKI
                     ISIYKATGAAHAKTHYQMRFLFDREKPFERCKIIE"
BASE COUNT          586 a          374 c          411 g          664 t
ORIGIN      
        1 cgcacaggtg accttggctg cttcgataaa catggttacc tctatattaa aggacgattg
       61 aaaaatatga tcgtgggagc aagtggtcaa aatatatatc ccgaagaaat tgaggctatt
      121 atcaatcggt tcaaatatgt acttgagtcg gttgtagttc agaagaaagg gaaacttgtg
      181 gctatggttc atttcaatat ggaggaactt gaagccaagt atacaaatct taaagatcag
      241 gcgagtcaac tcttcgagaa gaagattgaa gaactgtcag ttgagctgca gaactatgtc
      301 aattctcagg ttaataaatt ttcacgggtt caattggtta ttgtacatcc cgagcctttc
      361 gaaaagactg caacaaagaa gatcaaacgt tttctgtatt attaaaaaat gatataaaac
      421 cgaaaagccg ttccaaaatg gagcggcttt ttttgttgtc ttcttttatg agttgataca
      481 agaaaagccc caccattata ggcgaggctt ttcttgagta tctttagtat tgcttacttg
      541 cgattttcca tgtgcttagc atacctgttc atgaacttgt caacgcgacc tgcggtgtca
      601 accaacttca tcttaccagt gtagaacggg tgcgatgaac ttgaaatttc taacttgaac
      661 aatggataag tcacaccatc aatctcaata gtttctttcg ttgaagcagc tgatttagtg
      721 ataactactt cttcgttcga catatctttg aacgctacta aacgataatt cttagggtga
      781 ataccttctt tcattttact gcgcttatga tttctaattt ataacgggtt gcaaagatag
      841 gtattcattt aaattgtgca aaacattttc tgaaaacatt ttcaaggttt ataaaatatt
      901 tattttaaac ttgtatattt ctgttattca attattttac atctttcaaa tggtttttct
      961 ctgtcgaaaa gaaagcgcat ttggtaatgt gttttggcat gagctgctcc agtggcttta
     1021 taaattgaaa tgatttttgg gttgaaatcg cctgcccaag atatttctac ttccttatat
     1081 tgaggcttat gcagcattgc atcattctcc atgtgccaga atatggccga ctcaattcca
     1141 gacttctgaa atttttctac aatacccatg atgatgattc gggtccgtgt aaacttgcct
     1201 ttttttaaga aatacaatag tttcagaaaa ttcaacaggt taaggctccc gttcttaata
     1261 tagcgtaagg cttggttaaa atcgggaagc attaccaata gggcaatggg ttcgttgttg
     1321 tgatatgcaa accaaacaaa ttcggggtcg aggattaact tcgccgaatt caggaacgat
     1381 tttagttcat aatggtcaat ggccttaaaa tgctcgtgtt tctgccaagc cccttcatac
     1441 acggtacaga aatcatttat gaatttttcc gagtctttcc agtcgaagtg tttgaaagaa
     1501 aactgtggtt ttttagctac ccattcagca attttccaga aacgttcggg gaatggttta
     1561 gtatagtcga tatgatatga atattgttca taatagacat tgaacccgta atttttgaac
     1621 aactccacat aatagggcgg gttgtatggc attccgtacc cttgaggcat aaaaccccaa
     1681 gcaaggaggc cgtggttcat gtagttttcg cccatattgg cggggccgtc catggcctgc
     1741 ataccatggg tgccgagcca ttctcttgct ttatcgaaaa gaaggttggc tacatgttgg
     1801 tcgttggtgc attcaaaaaa accaatattc cctgttggct gttcaaagcg ttgggctttg
     1861 tcgaaatcga taaatgccgc aatacgtccc agtggttttt cttttgaatc aattgctatc
     1921 catcggatag ctttcccgtg tttgaaaaag gaattctttt cgggatcgaa attctcttca
     1981 accataccca ccaaaggaaa ggtgaagttt ggatcatctt tgtagattat cttgg
//
LOCUS       DAOPUW010000355         2034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_59685_length_2034_cov_4.542193, whole genome shotgun sequence.
ACCESSION   DAOPUW010000355 DAOPUW010000000
VERSION     DAOPUW010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59685_length_2034_cov_4.542193"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="PKJ43_06520"
     CDS             complement(<1..1357)
                     /locus_tag="PKJ43_06520"
                     /inference="COORDINATES: protein motif:HMM:NF025861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 N-terminal
                     domain-containing protein"
                     /protein_id="HNZ72257.1"
                     /translation="MKSRKQLMIATVLAAMLLWSCSNSKGQSQGISSPDGKVKITVNN
                     HDGQIFYAVDKEETKIVLPSKLGFVLKDGRVIGKKMKITNVKKTSFNETWEQPWGEKR
                     LIENRYEEMTVSLKSNAAKIAINLIFRAYNDGIGLRYDIPSQASIDSFIIMDELTEFA
                     LAGDENAWWTTAYKGVYYENITQLTPVSQMNDTVSLPLTLETQSNKFLAIHEANLTNY
                     AAMNLYASHGTTLKCDLTPWSTGEKVFAKTPFVSPWRMIIIADRPGDLLTSSLELNLN
                     DPCKIDDTSWIETGRYIGIWWGMHMEKYTWHMGPKHGATTENTMRYIDFAAQHGFSGV
                     LVEGWNTGWENDWTKEGDKFNFTQPYPDFDIEKITAYGKQKGVKLVGHHETGGATINY
                     EKQLDDAFAFYQKLGVNCVKTGYVSPLLDGKERHSSQYGVLHFRKVIETAAKYHIMIV
                     NH"
     gene            complement(1413..>2034)
                     /locus_tag="PKJ43_06525"
     CDS             complement(1413..>2034)
                     /locus_tag="PKJ43_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNZ72258.1"
                     /translation="FQFNYSWDFQSKLVSIFKNKTAANQLATTHMAEMSQVPEGRNKL
                     RYITNHDIYAWEGSPYEQFGNNEAALAAFAATVFSGGIPLIYSGQETGYQEKISFFEF
                     NPIEWDNTPTITSEIKQIIKIRKENQDFINTSFTSYSTSDIISFLHKKSNSELLVLIN
                     TRGGNASFTFPTELQNKQWTNLINNTNTEYSTKTEMMPYQYIILKR"
BASE COUNT          470 a          421 c          427 g          716 t
ORIGIN      
        1 cgtggttcac aatcataata tggtatttgg ccgcggtttc aattacttta cggaagtgca
       61 gcactccgta ctggctgcta tgacgctctt ttccatcgag taacggactc acatacccgg
      121 tcttcacgca gttaacccct aatttctgat aaaaggcaaa ggcatcgtcg agttgttttt
      181 cataattgat cgttgcgcct ccggtttcgt gatggccaac taactttacc cctttttgtt
      241 tcccgtaggc tgtaattttt tctatgtcga aatcggggta gggttgtgta aagttgaatt
      301 tatctccttc ttttgtccaa tcgttctccc acccggtatt ccagccttca accagcacgc
      361 ccgaaaaacc atgttgagca gcaaaatcga tataacgcat ggtgttttct gttgtcgctc
      421 catgtttagg tcccatatgc caggtatatt tttccatgtg cattccccac caaattccta
      481 tgtaacgccc ggtctcaatc cacgaagtat catcaatttt acaagggtcg ttcaggttta
      541 attctaacga cgaggtgagc aaatcgccgg gacggtcggc aatgattatc attcgccatg
      601 gtgaaacaaa cggggttttt gcaaatactt tctcgccggt cgaccatggt gttaaatcgc
      661 atttcagtgt agttccatgt gatgcgtaga ggttcatggc tgcataattt gtcaggttgg
      721 cttcatgtat tgccaagaat ttattgcttt gggtttcgag ggttaggggt agcgaaacgg
      781 tgtcgttcat ttgactaacc ggagtaagtt gggtaatatt ttcgtaataa acccctttgt
      841 aggcagtagt ccaccacgca ttttcatcgc ccgctaaggc aaattcggtc aactcatcca
      901 taatgataaa tgaatcgatt gaagcctgtg acggaatatc ataccttaat ccaatgccat
      961 cgttgtatgc tctgaaaatc aggtttattg ctatttttgc tgcattgctt ttcaacgaaa
     1021 cggtcatctc ttcatacctg ttttcaatga gtcgtttttc gccccaaggt tgttcccagg
     1081 tttcgttgaa cgatgttttt ttcacgttgg ttatcttcat ttttttgccg ataaccctgc
     1141 cgtctttcag cacaaatccc aattttgaag ggagtacgat ctttgtttct tctttgtcta
     1201 cagcatagaa aatctggcca tcgtggttgt ttactgtaat tttcactttc ccatcgggag
     1261 aactaatccc ctgactttgt ccttttgagt tgctgcatga ccataatagc attgcggcaa
     1321 gtactgtggc tatcatcaat tgttttctgc tcttcatatt ctttagggtt tatttctgtt
     1381 attcttatgt attgttaatt gattcgtctg ctttatcttt tcaatataat atattgatat
     1441 ggcatcattt ctgttttggt actatattct gtatttgtat tgttgatcaa gtttgtccat
     1501 tgcttgttct gtagttcagt cggaaaagta aacgaggcgt ttccccctcg ggtgttgata
     1561 aggaccagca gctcactatt gcttttctta tgaaggaacg aaatgatgtc ggatgttgaa
     1621 tatgatgtaa atgatgtgtt tatgaagtct tgattttctt tgcgtatctt tattatctgt
     1681 tttatttcac tggtaattgt tggggtattg tcccattcaa tggggttaaa ttcgaaaaag
     1741 gaaatctttt cctgataacc agtttcctgc ccggaatata tcagcgggat tccgccagag
     1801 aacacggttg ctgcaaatgc cgcgagcgct gcttcgttgt tgccaaattg ttcgtaaggc
     1861 gatccctccc aggcatatat gtcgtgattc gtgatataac gtaatttatt ccgcccttcc
     1921 gggacttggc tcatttcggc catgtgtgtt gtagctaatt ggttggctgc ggttttattt
     1981 ttgaaaattg aaaccaattt cgattggaaa tcccaactat agttgaattg aaag
//
LOCUS       DAOPUW010000356         2034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_59702_length_2034_cov_2.760990, whole genome shotgun sequence.
ACCESSION   DAOPUW010000356 DAOPUW010000000
VERSION     DAOPUW010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59702_length_2034_cov_2.760990"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PKJ43_06530"
     CDS             complement(<1..657)
                     /locus_tag="PKJ43_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNZ72259.1"
                     /translation="MGRVLIIGAGGVGTVVAHKMAANPDVFSNIMLASRTKSKCDAIA
                     EAIGGNRIETARVDANNVPELVALIKSYQPDIVVNVALPYQDLTIMDACLETKTAYLD
                     TANYEPENEAKFEYSWQWAYRDKFKNAGITAILGCGFDPGVTSIFTAYAAKHHFDEIH
                     YLDIVDCNAGDHGKAFATNFNPEINIREVTQKGKYWEDGEWKLTEPHEIHKPLNYPNI
                     G"
     gene            complement(917..1822)
                     /locus_tag="PKJ43_06535"
     CDS             complement(917..1822)
                     /locus_tag="PKJ43_06535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNZ72260.1"
                     /translation="MKTTIEVKKVYLIYNPLSGNKRSQSILDKILPIFEKGHIETQII
                     ETQYAGHARELANTLPFDGFDGLCVVGGDGTMHEIVNGLLKRADKMKIPIGLIPGGTG
                     NSFMHDVDALDPELAALRILTGRLRNVDIAQVETGGEIIYGFNIVGWGLPTDILQTAM
                     KLKWLGGQCYNIASLVEVIRNKPRLAKVKIDKQNIAGDYGFILGCNTIYTGKGMKMAP
                     LAQTNDGLIDLIVVRKANRLHLLYLFTKLFKGKHIGDPAVVYHQAHTFAIEPIENHTL
                     NIDGELIGCTPVHVKMLPGAVQVLV"
BASE COUNT          495 a          415 c          440 g          684 t
ORIGIN      
        1 gccaatgttg ggatagttca ggggtttgtg gatttcgtgt ggttctgtta atttccactc
       61 tccatcttcc caatatttac ctttctgggt tacttcacgg atgttgattt cagggttgaa
      121 attggtagca aaggcctttc catgatcacc tgcattgcaa tcgacaatgt cgagataatg
      181 gatttcatcg aagtgatgtt tggcagcata ggcggtgaaa atgctggtaa cgcccgggtc
      241 gaacccacat ccaagaatgg cggttatgcc tgcatttttg aacttgtcgc gatatgccca
      301 ttgccagcta tattcaaatt tagcttcgtt ttctggctcg tagttggcag tatccaaata
      361 ggctgttttt gtttccagac aggcatccat aatggtaagg tcctgatagg gaagggctac
      421 gttaaccaca atatcgggtt ggtagctctt gatgagtgca accagttcag gtacattgtt
      481 ggcatcaaca cgggctgttt caatcctgtt gcccccgata gcttctgcga tggcatcaca
      541 tttcgacttt gtgcggcttg ccagcatgat atttgagaaa acatcagggt tggcggccat
      601 tttgtgagca actacggtgc ctacaccgcc tgctccgata attagtactc gtcccacttt
      661 tatttttatc ttgtttataa aacgaatatg ttttgcggct caaatatgtt acttttttcg
      721 tatccgcaac catcaaattt agtgattttt atcaggctaa ttgcacgttt ttttgcaata
      781 gtgttaatgc actgattatg ttcggttaat ttattgccag gaaattctcc gggaaaataa
      841 ttacctcttt atgtgaaaat caatttacaa cttactaaaa cgttttttgg cagtgaaggt
      901 ttgaatgata cctaaattaa acaagtacct gaacggcacc cgggagcatt ttaacatgta
      961 caggtgtgca gccgataagt tctccatcga tattcaatgt gtgattttca atgggttcta
     1021 ttgcgaaagt atgggcttgg tggtatacca ctgcgggatc gccaatatgt tttcctttaa
     1081 atagtttggt aaataaatag agcagatgca ggcgattggc ttttcgcaca acaatcaggt
     1141 cgatcaaccc atcgttggtt tgggccagcg gagccatttt cattcctttt ccggtgtaaa
     1201 ttgtattgca cccgagtata aatccatagt cgccggcaat gttttgtttg tcaattttaa
     1261 cctttgccag tcttggttta tttctaatta cttcaaccaa tgaggcaatg ttgtagcatt
     1321 gtccaccaag ccattttagt ttcatggctg tttgtaaaat atcagtagga aggccccagc
     1381 caacaatgtt gaagccatat attatttcgc cgccggtttc tacttgggca atgtcgacgt
     1441 ttcgaagcct accggtgagg attcttagtg ctgccagttc tgggtcgagg gcatcaacat
     1501 cgtgcatgaa tgaatttcct gtacccccgg gaattaaccc aatgggaatt ttcattttat
     1561 cggcacgttt aagtaagcca ttcacaattt cgtgcattgt accgtcgccg ccaacaacgc
     1621 aaagcccgtc gaaaccatcg aacggcagtg tattagccaa ttcgcgcgca tggcctgcat
     1681 attgtgtttc aattatttga gtttcaatgt gccctttttc aaaaatggga agtatcttat
     1741 ctagaataga ctggctacgt ttatttccac tgagtgggtt gtaaatgagg tatacttttt
     1801 tcacttcaat cgttgtcttc attgtttgtt ctcactatca aaatggttag gttaaaaata
     1861 ctcattttga acaaatattc cacgaatgtg cagctattca ttcatactgt gttttcctac
     1921 attttattta gctgtgtttt tcagccggat tatccctgtt tggagccctt ttgggacggc
     1981 ggaaattttt cctgttctgt ttgaaaattc gttgttccga tgggggttct gcct
//
LOCUS       DAOPUW010000357         2033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_59740_length_2033_cov_3.565723, whole genome shotgun sequence.
ACCESSION   DAOPUW010000357 DAOPUW010000000
VERSION     DAOPUW010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59740_length_2033_cov_3.565723"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="PKJ43_06540"
     CDS             complement(<1..1024)
                     /locus_tag="PKJ43_06540"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNZ72261.1"
                     /translation="MNHPLFKKPAFLIWYGAFWMILSAIQFFLDLRVFEANLSIALVD
                     VLVSNTIFGMLGLGIWYVVVGGTNEKRSQIYLVTYHLIAMLIIVSFWLWLTNQIDVAL
                     FNRESIKAHMHKVLPGKAGVGALIYILISVFYYSFYYYERLKEKSQEETRLKELITQT
                     QLSELKSQINPHFLFNSLNSVSYLTISEPEKAQDMVIKLSTFLRYSLKHKENQLVSLS
                     QEMEHIKLYLEIEKVRFGDKLEAVFDITDCQGCQIPNMILQPLYENAIKYGVYETTHQ
                     VVIHTKAKKADNKLIFTIANNYDQDSVAKKGEGIGLLNIRKRLGLVYGDTTLLQISNE
                     NQIFTVT"
     gene            complement(1036..1791)
                     /locus_tag="PKJ43_06545"
     CDS             complement(1036..1791)
                     /locus_tag="PKJ43_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72262.1"
                     /translation="MNKSQFHFGRRFYFGFILLLIGLLMILERSGIISYELYDFLISW
                     KMFLVAIGAFVFVNGNRGAGIIVMGIGAFAMLPDIFEDYETIRRLFWPVLLLLAGLTF
                     MFGNRSKKLHRPPFNPHAQNMAQNPEINEVSNDYFDDMIIFGGKEISMLTSNLLGGRS
                     TAIFGGIEIDLRKCQISPEGCTIDATTLFGGNTLKVPNDWTILNKVVVLFGGYNDLRI
                     KDPSFQPNPAKTIVLTGVCIFGGIEVRSFNKIH"
BASE COUNT          614 a          388 c          406 g          625 t
ORIGIN      
        1 aggtaactgt aaatatttga ttttcattcg atatttgcaa taaggttgtg tctccatata
       61 caaggccaag ccgctttctg atattaagca acccaattcc ttcgcctttt tttgctacag
      121 aatcctgatc gtaattatta gcaatcgtaa aaatcaattt attgtcggct ttctttgctt
      181 ttgtatggat caccacctgg tgagtggttt cgtacacccc gtattttata gcgttttcgt
      241 aaagtggttg caatatcata ttgggaattt gacatccctg gcaatcagta atatcgaaaa
      301 cggcctccaa tttatcgcca aaacgaacct tttcaatttc gagatataat ttaatgtgtt
      361 ccatctcctg cgaaagtgaa acgagttgat tctctttgtg ttttaacgaa tagcggagaa
      421 aggtagagag tttaattacc atgtcttgtg ccttttcagg ttccgaaatt gttaggtacg
      481 aaacagagtt tagactgttg aaaagaaaat gcgggttaat ttgtgatttt agttcgctta
      541 gctgggtttg agtaatcagc tctttcagtc gtgtttcttc ctgcgatttc tctttgagcc
      601 tttcgtaata gtaaaacgaa taatagaata ccgaaatgag aatataaatc agggcaccaa
      661 ctccggcttt tcctggcaac accttgtgca tgtgtgcttt tatcgattcg cgattaaaaa
      721 gtgcaacgtc gatttggttg gtaagccaaa gccagaacga tacaatgatc agcatcgcaa
      781 taaggtgata agtaactaaa taaatctgag agcgtttttc gttagttcca cccacaacca
      841 cataccaaat tcctaaacca agcattccaa aaatagtgtt agagaccaaa acatcgacca
      901 acgcgatgga tagatttgct tcgaaaaccc gtaggtcgag aaaaaactgg atggccgaaa
      961 ggatcatcca gaaagctccg taccaaatta ggaatgctgg tttcttgaaa agtggatggt
     1021 tcatttgttt gattattagt ggattttatt gaagctacgg acttcaattc cgccaaaaat
     1081 acaaacacct gtaagcacta tggttttagc cgggttgggc tgaaacgaag gatctttgat
     1141 acgtaaatca ttgtaaccac caaagagtac cacaacttta ttcagtatag tccagtcgtt
     1201 gggtactttt agtgtgtttc cgccaaagag tgttgttgcg tcaatggtgc atccttcggg
     1261 cgaaatctgg catttgcgca aatcaatttc gatgccgcca aaaatggctg ttgaacggcc
     1321 gcccagcaag tttgatgtta gcatgctgat ttcttttccg ccgaaaatga tcatatcgtc
     1381 gaagtagtcg ttgctaactt cattgatttc cgggttttgt gccatgttct gagcatgtgg
     1441 gttaaacggg ggtctatgca attttttact tcgattaccg aacataaatg taagtcccgc
     1501 tagcaacaaa agcacaggcc aaaacaggcg cctgatggtt tcgtagtctt caaaaatatc
     1561 ggggagcatg gcaaaagcac cgattcccat tactatgata ccggcacctc tgtttccgtt
     1621 aacaaagaca aacgcgccaa tggcaaccaa aaacattttc cacgaaatga gaaaatcata
     1681 caactcgtat gaaataattc ccgaacgctc aagaatcatc aacaacccaa tcaacaacaa
     1741 aataaaccca aaataaaatc tgcgtccgaa gtggaattga cttttattca taactgcgtg
     1801 ttttaatttg tgtcaaaaat agaaggttat ttttagtgac cgatgctaag ttcgataaag
     1861 aagtgaattt tatcggtgaa atgagaagtt tggcacatcg gtagaattat tggaaaattc
     1921 ttttacagat attgtaaaaa aagaacgatg aaacagttta atcacttcat cgttctttta
     1981 ggttattttt attggttttg ttgttttgcc caagtgtctt ttaacgatac tgt
//
LOCUS       DAOPUW010000358         2032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_59820_length_2032_cov_2.294385, whole genome shotgun sequence.
ACCESSION   DAOPUW010000358 DAOPUW010000000
VERSION     DAOPUW010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59820_length_2032_cov_2.294385"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..124)
                     /locus_tag="PKJ43_06550"
     CDS             complement(<1..124)
                     /locus_tag="PKJ43_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNZ72263.1"
                     /translation="MKNTALITGASGGIGYELAKVHASKGGDLILVARNLEKLNQ"
     gene            562..1971
                     /locus_tag="PKJ43_06555"
     CDS             562..1971
                     /locus_tag="PKJ43_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNZ72264.1"
                     /translation="MRLAKVDKPFIRRWIFAIAILFTTSASYSQLPEAQQIASKMNVG
                     WNLGNTLEAICGENAWGNPNTTQRFIDSVKAAGFNTIRIPVAWFCHSDTSESVIDEDW
                     LERVKEVVDYCINDSMYVVMNAHWDKGWLENRVNLDNQDQVNERQFAYWTQIATFFKD
                     YDEHLLFAGANEPNVENATGMQVLQTYHQTFIDAVRQTGGNNASRTLIIQGPSTDIEK
                     TNKLMTSLPTDIIENRLMLEVHYYTPYQFCLMEKDADWGKVFYYWGKDYHSKTDVAHN
                     ATWGEERDVEKLFAMMKTQFVDKGIPVILGEFLAIKRSNLSGDNMALHVASRQYFHQY
                     VVGSAIKHGIIPYYWDTGDYGSGLFNRHNGSKIDKDNLDAIMQGAKNQVSTVIHYKNI
                     EASYFPNPFTSTVTLNIDTPEKIDSIVISDSSGKLTEKIIGNQIKKSIIFGGSFTPGI
                     YLVQIFAENTEKSFKIIKQ"
BASE COUNT          696 a          399 c          388 g          549 t
ORIGIN      
        1 actggttcaa tttttctaag ttccgggcca ccaaaattaa atcaccacct ttcgaagcat
       61 gaacttttgc caattcatac cctatcccac ccgatgctcc ggtaattaac gctgtatttt
      121 tcatctattt ttttatttgc tataagttaa caagcccaat gaattaaagt ttatcaatat
      181 caatcaagat tgaatggtat gaaaatctta aatcggcaaa tataaaactc tcaaacgatg
      241 agctttccat tattattggt taaaatgaat gattaaaaaa taactttaaa atttgttgag
      301 gacatccaga tagcaatcat atccccttga ggattgattc tatggtaagt accctatgaa
      361 aacattaaca gccttaaatg tcgacaatgt aggggtataa tattcaagct acagtttttg
      421 tcaatagatt tcttgtttag gaaaaaagaa tgatcagcca ataaactata cttgttggca
      481 gattttgctg gcggattgtt gtgccatgat agcaactcat tcaaaagcaa gatattttag
      541 aaatacaaag aatatagaat tatgagatta gcaaaagttg ataaaccatt tattagacgt
      601 tggatctttg caattgccat actcttcaca acatcggcct cctatagtca gctgcccgaa
      661 gcacagcaaa ttgccagcaa aatgaacgtt ggctggaact taggcaacac acttgaagcc
      721 atttgcgggg aaaatgcctg gggaaaccca aacactacac aacggtttat cgactcggta
      781 aaagcagcgg gtttcaacac cataaggatc ccggttgcat ggttttgcca ctcagacaca
      841 tcagaaagtg ttatcgatga agactggctc gaacgggtaa aagaagtggt cgattattgc
      901 atcaacgaca gtatgtatgt ggtaatgaac gcgcactggg acaaaggatg gcttgaaaac
      961 cgggtgaacc tcgacaacca ggatcaggtt aacgaaagac agttcgcgta ctggactcaa
     1021 atagccacgt ttttcaaaga ttacgacgag cacttgctgt ttgccggagc caatgaaccc
     1081 aatgttgaaa acgcaactgg aatgcaggtt ctgcaaacct atcatcaaac atttatcgat
     1141 gctgtccgtc aaaccggagg gaataatgct tcgcggactt taatcataca aggcccatcg
     1201 acagacattg aaaaaacaaa taaactcatg acctcactgc ctaccgacat tattgaaaac
     1261 aggctgatgc ttgaagtgca ttattatact ccatatcagt tttgtctgat ggaaaaagat
     1321 gccgactggg gtaaggtatt ttactactgg ggcaaagatt accattcgaa aaccgacgtt
     1381 gcacacaatg ccacatgggg cgaagaacgc gatgttgaaa agctttttgc tatgatgaaa
     1441 acccagtttg ttgataaagg gattccggtt attttaggcg aatttctggc cataaaacgg
     1501 tcaaatctct cgggcgataa catggccctt catgtggctt cgagacaata ttttcaccaa
     1561 tatgtagttg gatcagcaat aaaacatgga attattcctt actactggga tacaggcgat
     1621 tatggttctg gcttattcaa ccggcataat ggttctaaaa tcgacaaaga caatctcgat
     1681 gcaattatgc aaggagcaaa aaatcaggtt agtacagtta tacactataa aaatatagaa
     1741 gcatcttact ttcctaatcc attcacatcg actgttacct tgaacatcga taccccggag
     1801 aaaatcgaca gcattgtaat ctccgattct tcaggaaaac taacggaaaa gatcattggg
     1861 aaccaaataa aaaaatctat aatttttggc ggatccttta cgccaggtat atatttagta
     1921 caaatttttg ctgaaaacac cgaaaaatcg ttcaagataa taaagcagta aatatcaaaa
     1981 aataccaaac aggaaaaata cgttaatcaa caaagcagcg tctgcaatca aa
//
LOCUS       DAOPUW010000359         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_59855_length_2031_cov_2.803644, whole genome shotgun sequence.
ACCESSION   DAOPUW010000359 DAOPUW010000000
VERSION     DAOPUW010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59855_length_2031_cov_2.803644"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PKJ43_06560"
     CDS             <1..342
                     /locus_tag="PKJ43_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72265.1"
                     /translation="NNNNKLINVTSLRHPRFELVFGGKNSERENECIDVDEFIGIKSF
                     KAKGKRLSTYQIETITEIEPLIVDEEIEDEVEEVESEQEKKPLPFEDIPFEIDRGNGT
                     EEDNPNQMTLF"
     gene            482..925
                     /locus_tag="PKJ43_06565"
     CDS             482..925
                     /locus_tag="PKJ43_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNZ72266.1"
                     /translation="MNLSFVDLDAFIEEKYHKTIPEIFASDGEEGFRKAEQKALHEVA
                     DFEDVVVATGGGAPCFFDNMEKIKHSGTSLYLNGSPRILAERLLHSKTERPLIKGKTE
                     EELVAFINETLAKRNHWYKQADVIIDFDRDISTSEVIAALGSHKR"
     gene            1017..>2031
                     /locus_tag="PKJ43_06570"
     CDS             1017..>2031
                     /locus_tag="PKJ43_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNZ72267.1"
                     /translation="MKHLSSPNSLCTGIRVRMFVAILFVGAIFNACTPKEKSYKTENQ
                     IDANGYSFSQVTNDPLKTRIYTLKNGLKVYLTQNVDEPRVSCLVGVRAGSTSDPDSTT
                     GLAHYFEHMMFKGTSKLGTVNWSEESKQITQLEQLFEQYRSETDQGEKRKIYKEIDEV
                     SQQAATYVATNEYDKLMSAIGAKSTNAGTSYESTVYINDVPVNELERWAILESERFHD
                     IVLRLFHTELETVYEEFNMYQDMDSERAFEALMSDLFAGHPYGRSVIGLPEHIKNPSM
                     VNIKKFFETYYVPNNMAITMSGDIDFEKTIQLVDRYFGDLKYRELPKPQNYELQALSG
                     SVVK"
BASE COUNT          662 a          347 c          441 g          581 t
ORIGIN      
        1 aacaataata acaagcttat aaatgtcact tcgcttcgac accctcgttt tgaattggtt
       61 tttggaggca aaaatagcga gagggaaaat gaatgtatcg atgtagatga atttattggt
      121 attaagtcgt ttaaggcaaa aggcaaacgg ttgtcaacgt atcagattga aacaattacc
      181 gaaattgaac cattgattgt tgatgaagaa atagaggatg aagtggaaga agtggaaagt
      241 gaacaggaaa agaaaccact tccgtttgag gatattcctt ttgaaattga ccgtggaaat
      301 ggtaccgaag aagataatcc gaaccagatg acattgttct gattgggcac aacgttcaat
      361 ttctgataat actgtttaat aagaattata acttaatttg tcatataacc gatatgcgtc
      421 tgtttttaat aggatatatg gggagcggga aatcgactgt gggtaagggg cttgctaagc
      481 attgaacctt tcgtttgtcg atctcgatgc ttttattgaa gaaaaatacc acaaaaccat
      541 ccccgaaatt tttgcatccg atggtgaaga agggtttcga aaggcagaac aaaaagcctt
      601 gcacgaggtt gccgatttcg aagatgttgt ggttgccacc ggaggcggtg caccctgctt
      661 ttttgataac atggaaaaga taaaacactc tggcacttcg ctttatctta atggctcgcc
      721 acgaatcctt gctgaacgtt tattgcactc gaagaccgaa cggccgttaa tcaagggtaa
      781 aactgaagaa gaacttgttg ctttcatcaa cgaaaccctt gctaaacgta atcattggta
      841 caaacaagcc gatgttatta tcgattttga tcgtgatata agtaccagcg aagtgattgc
      901 tgcacttggt tcacataaac ggtaaaatca taaaataata ttgtataaca caatggtaaa
      961 ttattcataa ttttatcgtt gtgtttttta ttgtttcaca aaaattaaaa tagtccatga
     1021 aacacttatc atcacctaac tctttatgta ctggcatacg ggtacgcatg tttgttgcaa
     1081 ttcttttcgt gggggcaata ttcaatgcct gcactcctaa ggaaaaatca tataaaacag
     1141 aaaatcaaat tgatgcgaat ggatattcat tttcgcaagt aacaaacgac ccgttgaaaa
     1201 caaggatata taccttgaaa aacgggttaa aggtttatct tacacaaaac gttgatgaac
     1261 caagggtttc gtgtcttgtg ggggtgcggg ctggttcaac ttctgatcct gattctacta
     1321 ctggcttggc tcattatttc gaacatatga tgtttaaggg aaccagtaag ttaggaactg
     1381 taaattggag cgaagagagt aagcagatta cccaactgga gcaacttttt gagcagtata
     1441 ggtcagaaac cgaccaagga gaaaagagaa aaatctacaa agaaattgat gaagtatcac
     1501 aacaagcagc tacgtatgtg gctaccaacg agtacgataa acttatgtcg gcgattgggg
     1561 caaaatcgac taatgcaggt acttcatacg aatcaacagt gtatataaat gatgttccgg
     1621 taaatgaatt ggaacgatgg gcaatactcg aaagtgaacg ttttcatgac attgtacttc
     1681 gcctttttca tactgagttg gaaacggtat atgaagagtt taacatgtac caggatatgg
     1741 attcggagcg ggcattcgaa gccctgatgt ctgatttatt tgcagggcat ccatacggtc
     1801 gcagtgttat tgggttaccc gagcacatta aaaacccttc aatggtcaat ataaagaagt
     1861 tttttgaaac ttattatgtg cctaacaata tggctattac aatgtctggt gatatcgact
     1921 ttgaaaaaac aattcagctg gtcgaccgat attttggtga tctcaaatat cgggaattac
     1981 cgaaacccca aaattacgag ttacaggcgc tttctggctc agtggtcaaa g
//
LOCUS       DAOPUW010000360         2028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_59965_length_2028_cov_3.619361, whole genome shotgun sequence.
ACCESSION   DAOPUW010000360 DAOPUW010000000
VERSION     DAOPUW010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59965_length_2028_cov_3.619361"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="PKJ43_06575"
     CDS             <1..804
                     /locus_tag="PKJ43_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNZ72268.1"
                     /translation="TLNSVGIEMDNRYELLKSSNTRNIKEYNKKFISRKLNPEKGHRF
                     LPYIVVVIDEFADLIMTAGKEIEFPIARIAQMARAIGIHMVIATQRPSTNIITGVIKA
                     NFPSRIAFKVASMIDSRTILDQPGANQLIGKGDMLFSAGSDVTRLQCALVDTPEVERI
                     VEHIRSQQSYPTAYLLPEFLGVDGDMPGAVDLNQKDEFFDDAARLVVLNQQGSTSMIQ
                     RKFSIGYNRAGRIMDQLEAAGIVGPSEGSKARRVMYQDEYSLEQKLNSL"
     gene            929..1567
                     /locus_tag="PKJ43_06580"
     CDS             929..1567
                     /locus_tag="PKJ43_06580"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNZ72269.1"
                     /translation="MKKIILAILTIISLQSFSQSDAKAKAVLDKVSEKTRNYPSITAS
                     FDFTMQNNAAGLNETTGGNLILQKDKYKLTMSGVEIFCDGKTQWTYMREAQEVSINNA
                     GSESGELLNPATVFTIYEKGFKNTYLGEFTNSGKKTCKIELLPHTVKEFSRLILEIDL
                     SNYQIMNAKMYGTDDNEYTIKIKSMTTTTNYEPSTFIFDTKKNSNIEVIDMR"
     gene            complement(1573..1989)
                     /gene="aroQ"
                     /locus_tag="PKJ43_06585"
     CDS             complement(1573..1989)
                     /gene="aroQ"
                     /locus_tag="PKJ43_06585"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNZ72270.1"
                     /translation="MYIKIINGPNLNLLGIREKGIYGDQSFEPYVSNLQNKYPELIID
                     YFQSNVEGEIINYLHQVGFSCDGIIINAGAYTHTSIAIRDAISAIKAPVVEVHISNIL
                     TRENFRHESLIGPVCRGSIMGFGFDSYRLAIESFLE"
BASE COUNT          711 a          404 c          394 g          519 t
ORIGIN      
        1 acacttaact cggttggcat tgaaatggac aaccgttatg agcttttaaa aagttcaaat
       61 acccgaaaca taaaagaata caataagaag tttatatcaa gaaagctcaa ccccgaaaaa
      121 gggcatcgtt ttctgcccta tattgtggtg gttatcgacg aatttgcaga cttgataatg
      181 accgctggaa aagaaattga atttcccatt gcccgcattg cacaaatggc acgggccatt
      241 ggtatacaca tggtaattgc cactcagcgg ccatcaacaa atatcattac cggggttata
      301 aaagccaact tcccttcaag aattgcattt aaagtagcct ctatgatcga ctccagaact
      361 attcttgacc aacccggggc aaaccagttg attggcaaag gagatatgct attctcagcc
      421 ggaagcgatg tgacacgact gcaatgtgcc ctagttgaca cgcccgaagt agagcgaatt
      481 gtagaacaca tacgttctca gcaatcatac cctacagcat atttacttcc cgaatttctt
      541 ggcgttgatg gcgacatgcc cggtgcagtt gaccttaatc aaaaagatga attcttcgac
      601 gatgctgccc gtttggtagt attgaaccaa caaggatcaa catcaatgat tcaacgcaaa
      661 ttttcgatcg gctataaccg tgcaggccga attatggatc aactggaagc tgcgggtatt
      721 gtaggaccaa gcgaaggcag caaggcacga agggtaatgt accaggacga atacagtttg
      781 gaacaaaaat tgaattctct gtagaaattt tgataaatac ataatacata ttagttttgc
      841 ggcatcgcaa ttttgctggt gcttaattgt tgtagaagga aaagagtgtg tgtagaaaga
      901 agacaacaaa aaattaggta gaaaggatat gaagaaaatt atcttagcaa tactgacgat
      961 catatcgtta caaagctttt cacaatcaga tgcaaaggca aaagccgtac tcgacaaagt
     1021 atcagaaaag acgaggaatt accccagcat tacagcaagt tttgatttta cgatgcaaaa
     1081 caatgccgca ggattaaacg aaaccaccgg aggaaacctg atcttgcaaa aagataagta
     1141 caagctgacc atgagtggtg ttgaaatttt ttgcgatggt aaaactcaat ggacttacat
     1201 gcgcgaagca caagaagtaa gcataaacaa tgctggcagc gagagtggag agttactcaa
     1261 cccggccact gtgttcacaa tctatgaaaa aggattcaaa aacacctatt tgggtgaatt
     1321 cacaaattcg ggcaagaaaa cctgtaaaat tgaattgctg ccccatacgg tgaaagaatt
     1381 ctcacgcctt attttggaga tcgatttatc gaactatcaa atcatgaatg ccaaaatgta
     1441 tggcaccgac gataatgaat atacaattaa aataaaatcg atgaccacaa caacaaatta
     1501 tgaaccatcg acttttattt tcgacacgaa gaaaaactcc aatattgaag tcatcgacat
     1561 gcgctaatga tgctactcaa gaaaactctc gatagccagg cggtacgaat cgaagccaaa
     1621 tcccataatg cttccccggc atacagggcc aataagcgat tcgtgcctga aattttcgcg
     1681 tgttaggata tttgagatgt gcacctcaac aaccggagcc ttaatggcag aaattgcatc
     1741 ccgaatagca attgaagtat gagtataagc cccggcatta attataatcc catcgcatga
     1801 aaaaccaacc tgatgaagat aattaattat ttcaccttca acattagact gaaaataatc
     1861 aattattaat tcaggatact tattttgtaa atttgataca tacggttcaa aactttggtc
     1921 tccatatatg cctttttccc gaatacccag taaatttagg ttagggccat taataatttt
     1981 tatatacata aatacctatc attcagttaa taaaacaaaa atacaaat
//
LOCUS       DAOPUW010000361         2017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_60488_length_2017_cov_3.845566, whole genome shotgun sequence.
ACCESSION   DAOPUW010000361 DAOPUW010000000
VERSION     DAOPUW010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60488_length_2017_cov_3.845566"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PKJ43_06590"
     CDS             complement(<1..590)
                     /locus_tag="PKJ43_06590"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNZ72271.1"
                     /translation="MKRILITGGAGFIGSHLCERLLHEGHDIICLDNYYTGSKQNIEH
                     LIGHPYFELVRHDVTQPFFAEVDEIYNLACPASPIHYQHNPIKTIKTSVMGAINMLGL
                     AKRVNAKILQASTSEVYGDPEVHPQHEGYWGNVNPIGPRSSYDEGKRCAESLFVNYHY
                     QNNVRIKIARIFNTYGLRMHPNDGRVISNFVTQAIMG"
     gene            complement(630..>2017)
                     /locus_tag="PKJ43_06595"
     CDS             complement(630..>2017)
                     /locus_tag="PKJ43_06595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HNZ72272.1"
                     /translation="CCIGAGYVGGPTMAVIALKCPNITVTVVDINPERIAAWNSDLLP
                     IYEPGLDEVVKKTRGKNLFFSTDVEKGIREAEMIFISVNTPTKTYGIGKGMAADLKYV
                     ELCARQIADVAQEDKIVVEKSTLPVRTAQTIRTVLTNNNTNGVEFSVLSNPEFLAEGT
                     AINDLLYADRILIGSEENLDGKKAEKALVDIYSNWIPEEKIITTRIWSSELSKLTANA
                     FLAQRISSINSLSALCEETGADIDEIARAIGTDSRIGPKFLKASVGFGGSCFQKDILN
                     LVYLCHYYNLNEVADYWEQVVIINNYQRTRFVNNIIKALFNTVSGKKIALLGWAFKKD
                     TNDTRETAAMYVARDLFTEQAELHIYDPKVSSAQIIRDLRSALEAIGQQTNEQYIAKN
                     IIIHNDYRQAVDKAHAIAIVTEWDEFKNYNWPQVFEGMKKPAFLFDGRNLLDIQEMKA
                     IGFNYRGIGKG"
BASE COUNT          500 a          419 c          418 g          680 t
ORIGIN      
        1 cccattatgg cttgtgttac aaagttagaa atgacccggc catcgtttgg atgcatacgt
       61 aggccgtaag tattgaagat tctggcaatt tttatcctga cattattttg ataatgataa
      121 ttaacgaaca atgattcggc acagcgtttt ccctcatcgt agctcgatcg gggacctata
      181 gggtttacat ttccccagta cccttcgtgt tgggggtgaa cctcggggtc accgtatact
      241 tcgctggtcg atgcctgcaa tatctttgca ttcacccttt ttgccaatcc aagcatgttg
      301 attgcaccca ttaccgacgt ttttattgtt tttatagggt tgtgctgata gtgaattggt
      361 gaggccgggc atgcaaggtt ataaatctcg tcaacctcgg caaaaaaagg ttgagttaca
      421 tcgtgccgaa ccaattcaaa atacgggtgg ccaataaggt gctcgatatt ttgtttgctc
      481 cccgtgtaat agttgtctag gcaaatgata tcgtgccctt catgtaatag tctctcacac
      541 aagtgtgaac cgataaaacc tgcaccaccg gtaataagta ttcttttcat tgttgctaat
      601 cgtttatagc tatttggttg aaagaaatat tatccttttc ctattcccct gtaattgaac
      661 cctattgctt tcatttcttg tatgtcgagc aagtttcgac cgtcgaaaag aaatgctggc
      721 tttttcatcc cttcgaatac ttgtggccag ttgtaatttt taaattcgtc ccattcggtt
      781 acaatagcaa ttgcatgagc tttgtcaaca gcttgacgat aatcgttgtg gataatgatg
      841 ttttttgcaa tgtactgttc gttagtttgt tgtccgattg cttccagtgc cgatcttagg
      901 tcacgaatta tttgggctga agataccttt ggatcataaa tgtgcaattc tgcttgttct
      961 gtaaataaat cccgggcaac atacatggct gctgtttcgc gagtgtcgtt cgtatctttt
     1021 ttgaatgccc agcctaataa ggcaattttt ttccctgaaa cagtgttgaa caacgctttt
     1081 ataatgttat ttacaaatct ggttctctgg tagttattta taattacaac ctgttcccaa
     1141 tagtcggcta cttcgttcag gttgtaatag tggcaaagat aaaccagatt tagaatatct
     1201 ttttgaaagc aactaccacc gaaccctaca gaggctttca gaaatttagg gccaatacgg
     1261 ctatcggtgc caatggcgcg ggctatttca tcgatgtcgg cacctgtttc ttcgcaaagg
     1321 gccgacaatg agttaataga tgaaatgcgc tgggctagaa aagcatttgc ggttagcttc
     1381 gaaagctctg acgaccatat gcgagtagtt attattttct cctcgggaat ccagttagag
     1441 tatatatcaa caagtgcttt ttctgctttt ttgccatcaa ggttttcttc acttcctatt
     1501 aaaattcgat cggcgtacag gagatcattg attgctgttc cttctgccaa aaattcagga
     1561 tttgacaaaa cgctaaattc aacaccattt gtgttgttgt tggtaagaac tgttcttatc
     1621 gtttgcgctg ttctgacggg caatgtcgat ttttcaacta caattttatc ttcttgggca
     1681 acgtctgcaa tttgtcttgc acaaagttca acgtatttca aatcggctgc catgcctttc
     1741 ccaattccgt acgtttttgt tggggtgttc accgatataa atatcatttc ggcctcgcgt
     1801 atgccttttt caacatcggt tgagaaaaag aggttctttc cgcgggtttt tttaactact
     1861 tcgtcaagtc ctggttcgta tattggtaac aagtcgctgt tccatgcagc tatcctttcg
     1921 gggttaatgt ctacaacagt tacggtaatg tttgggcatt taagtgcaat aaccgccata
     1981 gttggaccgc ctacatagcc ggcaccgatg caacata
//
LOCUS       DAOPUW010000362         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_60766_length_2011_cov_3.496933, whole genome shotgun sequence.
ACCESSION   DAOPUW010000362 DAOPUW010000000
VERSION     DAOPUW010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60766_length_2011_cov_3.496933"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1510
                     /locus_tag="PKJ43_06600"
     CDS             <1..1510
                     /locus_tag="PKJ43_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNZ72273.1"
                     /translation="SFTEKNFQKAKFATNDPFTELGLIDFTKIGVPITSLTKEALIDS
                     DMDNKAVLRSKNMLALGLVSWIFNRPLEIIEEALAAKFGKNPTVLQSNIDVLHAGYNY
                     GMNMEHETPQYLVPSGEIEKGIYRNISGNQATAWGFIAAAEKAGIELFLGSYPITPAT
                     DILHTLSERKDLGVKTFQAEDEIAGICSAIGASYAGALGITTTSGPGLALKSEAIGLA
                     VMAELPLVVVDVQRGGPSTGLPTKTEQSDLMQSLWGRSGESPVVVLAASSPSDCFHYA
                     YMASKIAIERMTPVILLTDGFLGNGSEPWKIPSMADMPPITKRMAVSQETYKPYRRPD
                     NTLAREWAIPGMEGFEHRIGGLEKDENGAVSHVPENHQLMCELRAEKVARCVDFVPNL
                     ETKGCTEGDVLIVGWGGTFGHLVSATRDLNLLGKKVGLAHFNYINPLPANTADVFSKY
                     KKIVVCELNLGQFVSYLRYMLPQFKYLQYNKIQGMPFAVEDIKENVLKILED"
     gene            1513..>2011
                     /locus_tag="PKJ43_06605"
     CDS             1513..>2011
                     /locus_tag="PKJ43_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNZ72274.1"
                     /translation="MSEFVYDAKDFKSSTEVRWCPGCGDHAILNAIQRAMASLGNKIS
                     DYAIISGIGCSSRFPYYMNTFGFHTIHGRASAIASGVKSANPKLHVWQITGDGDALAI
                     GGNHFIHLVRRNIDINVILFNNKIYGLTKGQYSPTTDRGFKTKSSPFGTVEDPFVPGQ
                     LVLGAR"
BASE COUNT          553 a          396 c          480 g          582 t
ORIGIN      
        1 ttcgtttact gaaaaaaact ttcaaaaggc aaaatttgca acaaatgacc cgtttaccga
       61 actgggtttg attgatttta caaaaattgg cgttccaatt acttcgttga ctaaagaagc
      121 gttgatcgat tctgatatgg acaacaaagc cgttttacgc agtaaaaaca tgttggcact
      181 tgggttggtt agctggatct ttaaccgtcc gctcgagatt atagaagagg cgttagctgc
      241 aaagtttggg aaaaatccaa cagtacttca gtcgaatatc gatgtacttc atgccgggta
      301 caactatgga atgaacatgg agcacgaaac gcctcaatat ttggttccat cgggtgaaat
      361 tgaaaaaggt atctatcgaa atatcagcgg aaaccaggct actgcatggg gttttattgc
      421 cgcagctgag aaagctggta ttgaactctt tctcggctca tatccaatta ctccggctac
      481 agatattttg cacaccttat cggaacgcaa agatttgggc gtgaaaacat ttcaggcaga
      541 ggacgaaatt gcgggcattt gttctgcaat cggagcttct tatgctggcg cgttgggaat
      601 tactactact tcaggtcccg gacttgctct taaatcggaa gctatcgggt tggccgtaat
      661 ggccgaattg ccactggttg tggttgatgt tcagcgaggt ggtccatcaa cggggttgcc
      721 taccaaaaca gagcagagcg acttaatgca atcgctttgg ggccgtagcg gcgaaagccc
      781 ggttgttgtt ttggctgcca gttcacccag tgattgtttt cattatgcct acatggcttc
      841 aaaaattgcc atcgaacgga tgaccccagt gattcttctt accgatggtt ttcttgggaa
      901 tggtagtgaa ccatggaaaa taccctctat ggccgatatg ccgcctatta ctaaacgaat
      961 ggctgtaagc caagaaacct ataagcctta taggcgaccc gataataccc ttgcccgcga
     1021 atgggccatt cccggaatgg aaggtttcga acaccgaatt ggggggctcg aaaaagatga
     1081 aaatggagct gtaagccatg tgcccgaaaa ccatcagctt atgtgtgagt tgagggctga
     1141 aaaagttgcc cgttgtgtcg attttgtacc caatctggaa actaaaggtt gcactgaagg
     1201 agatgtgctg attgtgggat ggggcggtac atttggccat ttagtcagtg ctactcgcga
     1261 cctcaatctg ttgggaaaga aagtaggact ggctcacttt aattacatca atccgctacc
     1321 tgccaatacc gccgatgttt tttcaaaata caaaaagatt gttgtttgcg aattgaactt
     1381 aggacagttt gtttcgtatc taagatatat gctgcctcag tttaaatatt tgcaatacaa
     1441 taaaatacag ggtatgcctt ttgccgttga agatattaaa gaaaacgtac ttaaaatttt
     1501 ggaggattag tcatgagcga atttgtatac gatgctaaag attttaaaag ttctacggaa
     1561 gtacgttggt gtccggggtg tggcgatcat gccattttaa atgccattca acgggctatg
     1621 gccagcttgg ggaataagat ttcggattat gcgattattt ctgggattgg atgctcctcg
     1681 cgtttccctt attacatgaa tacctttgga tttcatacca ttcacggcag ggcttcggcg
     1741 attgcttcgg gtgttaaaag tgcaaatccg aaattgcatg tttggcaaat taccggcgat
     1801 ggagacgctc ttgcaattgg aggaaaccac tttatccatt tggtacgccg aaatattgat
     1861 attaatgtga ttcttttcaa taataaaatt tatggtctaa caaaaggtca atattctcct
     1921 acaaccgatc gtggttttaa aaccaaatca tcgccatttg gtacggttga agatcctttt
     1981 gttcctggac agcttgtact tggtgcccga g
//
LOCUS       DAOPUW010000363         2010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_60828_length_2010_cov_2.583120, whole genome shotgun sequence.
ACCESSION   DAOPUW010000363 DAOPUW010000000
VERSION     DAOPUW010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60828_length_2010_cov_2.583120"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="PKJ43_06610"
     CDS             complement(<1..1070)
                     /locus_tag="PKJ43_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNZ72275.1"
                     /translation="MKIDPFLGNIDPKTLDGMYRDYLSNPDSVEENWRLFFKGFDLAR
                     QNYTNGHGELFDAEVKVLNLIDAYRKRGHLFTKTNPVRARQEHKPTLDLENFGLSSSD
                     LNTIFQAGKELGIGPAKLSDIVIHLQNTYCKSVGVEYMYIRDTEVVAWLKQKIEESQN
                     QTEFSREQKKHILYHLKLAVGFEQFIHKKFVGQKRFSLEGAESLIPALDAVVEKGAEL
                     GISEFIIGMAHRGRLNVLTNIMQKPYENIFNEFYGEQYDEDVALGDVKYHLGYSNSIK
                     TDTGKSVYLNLVPNPSHLETVTPVVLGLSRSRIDQVYGNDYGKLAPIVIHGDAAVASQ
                     GVVYESIQMSQLRGYKTGGTIH"
     gene            1170..1964
                     /locus_tag="PKJ43_06615"
     CDS             1170..1964
                     /locus_tag="PKJ43_06615"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNZ72276.1"
                     /translation="MPQNVAMAEYFSRSVCFFTIILNPFCIMTSIDRIEQELNELGNS
                     EKAKAMAKFFQVFPGGYGEGDSFIGVTVPNQRLIAKKYYREICLDDIENLLSTGIHEY
                     RLTALFMLVLKFDKAKDENQRESIVALYLKNIKWVNNWDLVDSSAHLILGPHLYTRSR
                     DMLYEMAKSPNIWIQRIAIIATLHFIRKGQFSDTLGIAELLLHHEHDLIHKAVGWMLR
                     EVGNRDYHTEYAFLKKHYKHMPRTMLRYAIERFPEPIRQDFLKGSI"
BASE COUNT          552 a          433 c          419 g          606 t
ORIGIN      
        1 atatggatag tgccacccgt cttgtaacct ctgagctgcg acatctgaat gctttcgtaa
       61 acaactcctt gcgaggcaac ggcggcatcg ccatgaatta cgatgggagc caatttgccg
      121 taatcgttac catacacctg gtcaattctc gacctcgaca acccaagaac cacgggtgta
      181 acggtttcga ggtgtgaggg atttggtacc agattaagat agacactttt cccggtatcg
      241 gtttttatgg aattgctata tcccaaatga tacttaacat cgcccagagc cacgtcctca
      301 tcatactgtt ctccgtaaaa ttcgttgaag atattctcat atggcttttg catgatgttc
      361 gttagcacat taagccttcc cctgtgcgcc atgccgataa taaattcgct tatgcccaat
      421 tcggcacctt tttctaccac tgcgtccagt gcagggatta acgactcggc cccctcaagc
      481 gaaaaccttt tctgacccac aaacttttta tggataaatt gttcgaatcc cacagccagt
      541 ttcagatggt atagtatatg ttttttctgc tccctcgaaa actccgtttg gttttgcgat
      601 tcttcaattt tttgtttcaa ccatgccacc acctcggtat ccctaatgta catgtattca
      661 acgccaaccg atttgcaata agtattttgc aagtggatga ctatgtccga aagttttgca
      721 ggaccgattc ccaactcttt tcctgcctga aaaatagtgt tcaaatccga tgaggatagc
      781 ccaaaatttt caaggtcaag ggttggctta tgctcttgtc gggcacgcac cgggttcgtt
      841 ttagtaaaaa gatgaccccg tttacggtag gcgtcaatca ggttgagaac ttttacttcg
      901 gcatcgaaaa gttctccgtg cccattggta taattctggc gggccaaatc aaatcctttg
      961 aagaataacc gccagttttc ttcgaccgaa tcaggatttg aaaggtaatc cctatacatg
     1021 ccatccagcg ttttggggtc gatgttgcct agaaatggat caattttcat tcttaataat
     1081 cgctttaatt gagggtgtaa gcaaagttat cagaaattat cggaaattct actgtttagt
     1141 ttttaagtgt atatttgaac taacagttca tgccacaaaa tgttgcaatg gctgaatatt
     1201 tcagtcgttc agtttgtttt tttacaatca ttcttaaccc gttttgcatt atgacttcca
     1261 ttgataggat tgaacaggaa ttaaatgagt tgggaaattc cgagaaggca aaagcaatgg
     1321 caaaattttt tcaggttttc cccggcggtt atggcgaggg cgattcattc ataggcgtta
     1381 cggtacctaa ccaaaggttg attgccaaaa aatactatcg tgaaatttgt ttagatgata
     1441 tagaaaatct cttatctacc ggaatccatg agtacaggtt gacggccctt tttatgctgg
     1501 tacttaaatt tgataaagcc aaagatgaaa atcagcgcga aagtatagtg gctctatacc
     1561 tgaagaatat caaatgggtg aacaattggg atttggtcga ttcctctgcc catttaatcc
     1621 tcggcccaca tttatataca cggagtaggg atatgttgta cgaaatggca aaatcgccaa
     1681 atatatggat tcagcgaata gccattatag caacccttca tttcatcaga aaaggccaat
     1741 ttagcgacac attggggatt gcagagttgt tgctgcacca cgaacacgat ttgattcaca
     1801 aggccgtggg ctggatgtta cgtgaggtgg gcaataggga ttaccatacc gaatatgctt
     1861 ttttgaaaaa gcactacaag cacatgcccc gcaccatgtt gcggtatgcc attgagaggt
     1921 ttcccgaacc gattagacag gattttctga aaggttctat ctgatgtttt ccaacttcct
     1981 gacaacgatt aatccagctc tctggaatgt
//
LOCUS       DAOPUW010000364         2010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_60841_length_2010_cov_2.045013, whole genome shotgun sequence.
ACCESSION   DAOPUW010000364 DAOPUW010000000
VERSION     DAOPUW010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60841_length_2010_cov_2.045013"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PKJ43_06620"
     CDS             complement(<1..216)
                     /locus_tag="PKJ43_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNZ72277.1"
                     /translation="MNPNLDPEQKLNNEDQEIERALRPQRIDDFKGQPQIIENLFIFI
                     EAAKRRGESLDHLLLHGPPGLGKTTLSH"
     gene            353..1282
                     /gene="queG"
                     /locus_tag="PKJ43_06625"
     CDS             353..1282
                     /gene="queG"
                     /locus_tag="PKJ43_06625"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNZ72278.1"
                     /translation="MLNAALSNQIKAQALALGFQDVGFAKAEGLDSEAKYLEQWLNQQ
                     RNGKMGYLENYFDKRVDPRLLVDGAKTVISLSYNYFTEQHQLDETAPKIAMYAYGADY
                     HVVVKEKLEQLLQFIREQSGDINGRCFVDSAPVLERAWAERSGIGWMGKNTNILTKQK
                     GSYFFLAEIIVDIELPYDSPVKNFCGTCTKCIDACPTEAIIAPYQIDGSKCISYFTIE
                     LKDEILPSDQKGKFDNWMFGCDVCQQVCPINSQAKPHNEPRFEPHPDLLSLTKNDWLH
                     LQQETFQQLFKHSAVKRTKFTGLKRNIAFLQTQ"
     gene            1353..>2010
                     /locus_tag="PKJ43_06630"
     CDS             1353..>2010
                     /locus_tag="PKJ43_06630"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HNZ72279.1"
                     /translation="MAKKRRKRSITSYVLWFNFMAIICIALYFHKPIIKYSFRAYRWY
                     ARKHNKGKISPADFPMQYEVHGIDVSHYQPDIDWEHLASIATNGDTVRFQFVFIKATE
                     GAWWEDELFAEHWENAKDNHIIRGAYHYFLPNKDAKKQAKNFIESVDLEKGDLPPVID
                     IEETKGMSKEAIVKAVKDYATVIEQHYNVKPIIYSNRNFIEDYLAEDFPDYTFWIAHY
                     Y"
BASE COUNT          595 a          379 c          439 g          597 t
ORIGIN      
        1 atgcgacaaa gttgtttttc ccaaacccgg aggaccgtgc aataacaagt gatccaacga
       61 ctcgcctctt cgcttggcgg cttcgatgaa aatgaaaagg ttctcaatga tttgaggttg
      121 tcctttaaaa tcatcgatcc gttgcggacg cagggctctt tcgatttcct gatcttcgtt
      181 gttgagtttt tgttccggat ctaaatttgg attcatgtgc tgcaaagatg cgtttgattt
      241 ctgtattttg tgtgaaaata atttgttttt tgaatcacat gcacaaagca atgaaattct
      301 ttgttgtaca acaaaagact ttactttcgc tgtataacaa ttctctctgc tcatgttgaa
      361 cgcagccctt tccaaccaaa ttaaggcaca agctttggct ttgggctttc aagacgttgg
      421 ttttgcgaaa gccgagggat tagatagcga agcgaaatat ttggaacaat ggctgaacca
      481 acagcgaaat gggaaaatgg gctatttgga aaattacttc gacaaacgtg ttgatccgag
      541 gttgttggtt gacggggcta aaacagtaat ttcgttatcc tataactact tcaccgagca
      601 acatcaactc gatgaaacag cgcctaaaat agcgatgtat gcttatggag ccgattacca
      661 tgtggtggtg aaagagaagt tagaacaatt gctgcaattt attcgggaac aaagtggaga
      721 tatcaacgga cgctgctttg ttgatagtgc tccggtgctg gaaagagcat gggcagagcg
      781 ttccggtatt ggttggatgg gcaagaacac caacatcctc acgaaacaaa agggttcgta
      841 tttctttctg gcggaaatca ttgtagatat tgaattaccg tatgatagtc cggtgaaaaa
      901 cttctgcggt acttgcacaa aatgtataga tgcttgtccg accgaggcca ttattgctcc
      961 ctaccaaatc gatgggtcga aatgtatttc ctatttcact attgaattaa aagatgaaat
     1021 actgccatcc gaccaaaaag gcaaattcga taactggatg tttggctgtg atgtttgtca
     1081 gcaagtttgc cccattaact cgcaagcaaa gccgcacaat gaaccacggt ttgaacccca
     1141 tccggatttg ttgagtttga ccaaaaacga ttggctccat cttcagcagg agacctttca
     1201 acaactcttc aaacattctg cagtaaagcg aacgaaattt acagggttga aaagaaatat
     1261 agcatttctt caaacccaat gatatcaacg ctttatcgcc aagtaaatcc tatcgtgcac
     1321 ggacatatca aatattttta tatttttatt tgatggcaaa gaagaggagg aagcgcagta
     1381 taacttcgta tgttttgtgg tttaatttca tggcaatcat ctgtattgcc ctctattttc
     1441 ataagcctat tattaagtat tcttttcgtg cttatcgttg gtatgcccgc aaacacaata
     1501 agggaaaaat ttctcctgct gatttcccta tgcaatatga agttcacggc atagatgtat
     1561 cgcattacca acctgatatt gattgggagc atttggcctc gatagccacc aatggcgata
     1621 cggttcggtt tcagtttgta tttattaaag ctacagaggg tgcgtggtgg gaagatgagt
     1681 tgtttgctga acattgggaa aacgccaaag acaatcacat tattcgtggc gcttaccatt
     1741 atttcttgcc caacaaggat gcgaaaaagc aggcaaagaa cttcattgaa tcggtagatt
     1801 tagagaaagg tgatttacca ccggtgattg atattgaaga aactaaaggc atgagcaaag
     1861 aggccattgt aaaagcggtg aaagactatg ctacagtaat tgaacaacat tacaatgtga
     1921 agccgattat ttattccaac cgtaatttta ttgaggatta tttagcggaa gattttcctg
     1981 attatacttt ttggattgcg cactattacg
//
LOCUS       DAOPUW010000365         2008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_60913_length_2008_cov_3.150538, whole genome shotgun sequence.
ACCESSION   DAOPUW010000365 DAOPUW010000000
VERSION     DAOPUW010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60913_length_2008_cov_3.150538"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /locus_tag="PKJ43_06635"
     CDS             complement(<1..156)
                     /locus_tag="PKJ43_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNZ72280.1"
                     /translation="MNDLLKSNFSGKEYEAGCDEAGRGCLAGPVFAAAVILPTNYELP
                     LLNDSKQL"
     gene            complement(153..1280)
                     /locus_tag="PKJ43_06640"
     CDS             complement(153..1280)
                     /locus_tag="PKJ43_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNZ72281.1"
                     /translation="MRIAVNTQHLLKDNLEGIGWFAHETLRRIVLNNPGHEFLFIFDR
                     KWDESFLYGPNVIPVSTAIPSRHPVLWYWHYQVDVPKILKKYKPQLFFSPDGWISLNT
                     NIPTVTTIHDINFVHRPGDLPYLVKKYYNYYFPKFAHFSKRIVTVSEYSKADIANTYK
                     ISPSKIDVAYNGCNQRFRESSEEVKELVKKSFTNEHPYFIYVGSLNPRKNILGLLTAY
                     ENFRKNSLMPYKLVLAGKPMWENSTINAKLSSMTYASDVIFTGRVSDEVMQLLLSSSE
                     ALVMPSFFEGFGIPVVEAMYCETPVICSNVTSLPEVAGDAALFIDPNKPDTIEKAMHQ
                     IANSNSLREELIAKGRLQREKFSWDNTASLVWQSIEKTISL"
     gene            1472..>2008
                     /locus_tag="PKJ43_06645"
     CDS             1472..>2008
                     /locus_tag="PKJ43_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72282.1"
                     /translation="MKRKFFSNLILLLTINVLVKPFWLFGIDRTVQVVTGDAYGLYYS
                     LLAFSQTLNILLDLGITNYNNRNIARYNQLLPKHMGNIMGIKFMLGVVYAVVCIGLGL
                     LFGYNSVQFHLLYFLIINQFLLQLIQYLRSNLTALHLFTVDSLVSVLDRVFMIAFCGI
                     LLFTNVTDQPFRIEWFVYV"
BASE COUNT          495 a          389 c          437 g          687 t
ORIGIN      
        1 gagctgcttc gagtcgttca gcaatggcag ttcatagttc gttggcagaa ttactgctgc
       61 ggcaaatacg ggtcctgcaa ggcagcctcg tccggcttca tcgcatcctg cttcatattc
      121 tttgcctgag aaattcgatt ttagcagatc gttcatagtg aaatagtctt ctctattgat
      181 tgccagacca aagaagctgt gttgtcccaa ctaaactttt cgcgttgaag acggcccttg
      241 gcgatcaact cttccctgag gctgttgctg ttggcaattt ggtgcatggc cttttctatt
      301 gtatccggtt tgttcgggtc gataaatagt gctgcatcgc ctgcaacttc tggtaaagat
      361 gttacatttg aacaaattac cggcgtttcg caatacatgg cttcaacaac aggaattcca
      421 aagccttcga aaaatgaagg cataaccaat gcttcggatg atgagagcag gagttgcatc
      481 acttcgtcgg aaacacgccc ggtaaaaatt acatccgaag cgtaagtcat actgctaagt
      541 ttggcattta tcgttgagtt ttcccacatc ggtttgcctg cgagtaccaa tttatatggc
      601 atcaacgaat tttttctgaa attttcatag gcagtgagca accccaatat gttttttctt
      661 gggttcaacg agccaacgta tataaagtac gggtgttcat tggtgaaact ttttttgacc
      721 agttctttca cttcttcgct gctttccctg aaccgctggt tgcacccgtt gtatgctacg
      781 tctatttttg acggggagat tttgtaagtg tttgcaatgt cggctttgct atattctgaa
      841 acggttacaa tccgcttcga aaaatgggca aactttggga aatagtaatt gtaatatttt
      901 ttcaccagat agggcaaatc gccggggcgg tgcacaaagt tgatgtcgtg tatggttgtt
      961 actgtgggga tatttgtgtt gagcgaaatc catccatcgg gagaaaagaa taactggggt
     1021 ttatattttt tcaggatttt aggaacatca acctgataat gccaatacca taaaaccgga
     1081 tggcgcgagg gaatggcagt agataccggg attacattgg ggccgtaaag aaaactttcg
     1141 tcccattttc gatcgaatat gaaaaggaac tcgtggccgg ggttattgag tacaatcctg
     1201 cgcaaagttt cgtgcgcgaa ccaaccaatc ccttccaaat tatctttaag taaatgttgt
     1261 gtgtttactg caattctcat gaaacaaaaa tagtgaaatc gattctcttt tcaatttttg
     1321 cctgtatttg ttctctttct ttttgcattt ctgtctttcg tttattggct ggtgtaccat
     1381 tgctggcttt gtttttcaat tttccccgtt gttttttcat tgcaaataag cgataaagat
     1441 tgttacttta cgcgcataaa agttagcagt tttgaaaagg aagttttttt ctaacctaat
     1501 tcttttactt acaataaatg ttctggttaa gccattctgg ttgtttggaa tcgacagaac
     1561 agttcaggta gtcactggcg atgcctatgg gctttactat tccttgctgg ctttttctca
     1621 aacgctcaat attttactcg atttaggaat aactaattac aataaccgga atattgcccg
     1681 ctacaatcaa ttgttgccta agcatatggg taatattatg ggtattaagt tcatgcttgg
     1741 ggtggtttat gcagtggtat gtataggcct tgggcttttg ttcggctata acagtgttca
     1801 gtttcatttg ctttatttcc tgattatcaa tcagttttta ttgcaattga ttcagtacct
     1861 gagatcgaac cttacggcgt tgcatctatt tacggtggat agtctggtgt cggttctcga
     1921 ccgtgttttc atgatagcct tttgtggtat cttgctattt accaatgtca cagatcagcc
     1981 tttccgtatt gaatggtttg tatacgtt
//
LOCUS       DAOPUW010000366         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_61715_length_1990_cov_2.858398, whole genome shotgun sequence.
ACCESSION   DAOPUW010000366 DAOPUW010000000
VERSION     DAOPUW010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61715_length_1990_cov_2.858398"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /locus_tag="PKJ43_06650"
     CDS             complement(<1..1385)
                     /locus_tag="PKJ43_06650"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNZ72283.1"
                     /translation="MAENPSSNVEKNKDQTIQRSGKITLKINGITTQVDPGTTLLIAA
                     EKLGLNIPTLCHNSEVEHFNSCMICLVKNNRNQKLIPSCSIAADEGMDITTFDEEIQE
                     ARQTGLELLLSDHVGDCEAPCTIACPAHMDIPTMNRLLAAGKFNEALEVVKQNIPLPS
                     VLGHICPAPCEGACRRKPIDGAVSICLLKRFAGDHGQYTEKPLPYTGHKVAIIGSGPA
                     GLSAAYYLQLKGVQCVVFDKASEAGGMLRTEIAEEKLPREVLNREIQAIAALGVHFQL
                     NTEINKDTFAKIQQDYNAVLIATGNITEELKYWGIEWFFKGLKANEDTFQTTIENVFA
                     AGSVLRTSKLAIRSLAQGKEAATSIWQMLNKQPIIGQPMRFNSKFGKLLASEYSEYLS
                     ESVDIKRIESQNNFNSGFSLEEVKKEAARCMHCDCRKSTNCKLRDYSDRYKAFQKRFS
                     YEERKPVRKQC"
     gene            1548..>1990
                     /locus_tag="PKJ43_06655"
     CDS             1548..>1990
                     /locus_tag="PKJ43_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72284.1"
                     /translation="METIRYFHQPTISESLGNGWYVLKKHFWWLLLVVIITSIFDGPS
                     AKYTYDSDGSLLKAIGAASLLIGFGTLIFLLVRPVVSWGAKRVFLQCARNENPNLSYL
                     FAGFQHNYFNIVLTNLLLTIIIVAGFLFLILPGIFLAIRLVFVPY"
BASE COUNT          481 a          407 c          413 g          689 t
ORIGIN      
        1 tggcattgtt ttctcacagg cttgcgttct tcatacgaaa aacgtttctg aaaagccttg
       61 tatcgatctg agtaatctct tagtttacag tttgttgatt ttcgacaatc gcaatgcata
      121 caacgggctg cttctttttt aacctcttca aggctgaacc cactattgaa attattttgt
      181 gattcaatcc gctttatatc aactgattcg ctcaaatatt ctgaatactc cgaggccaac
      241 agttttccga attttgaatt gaacctcatt ggttgaccaa taatgggctg tttattaagc
      301 atttgccaaa tacttgtggc ggcttccttt ccctgggcaa gcgatcgtat agccaatttg
      361 gaagttcgca aaacacttcc ggctgcaaat acattttcta tagttgtttg aaatgtatct
      421 tcgttggctt tcaacccctt aaagaaccat tcaatacccc agtatttcag ttcttcggtt
      481 atatttccgg tagcgataag gactgcatta tagtcttgct gaattttggc aaatgtatct
      541 ttgttgattt cagtattcaa ttggaaatga acaccaagcg ctgcaatagc ttgaatctcg
      601 cgattgagca cttctcgggg caatttttcc tccgcaattt cggtacgcaa cattcctcca
      661 gcttcactgg ctttatcgaa tacaacacac tgaactcctt ttagttgaag gtaataagcg
      721 gctgacaatc cggccggacc gcttcctatg atagctacct tgtgtcctgt gtagggcaat
      781 ggtttttctg tgtattgtcc gtgatcgccg gcaaatcttt tcagaaggca aatagaaacg
      841 gccccgtcaa ttggttttct tcggcaggct ccttcgcatg gagccgggca gatgtgcccc
      901 aacacagaag gaaggggtat gttttgtttc actacctcta acgcctcgtt aaattttccg
      961 gctgccagca aacgattcat agttggtatg tccatgtggg ccgggcaagc tatggtgcaa
     1021 ggggcttcgc aatcgccaac atggtctgaa agaagtaact cgagacctgt ttggcgggct
     1081 tcctgaattt cttcatcgaa ggtagttatg tccattcctt cgtcagcggc aattgagcat
     1141 gaaggaatta acttttggtt tcgattgttt tttacaaggc agatcatgca tgagttaaag
     1201 tgctcaacct ccgagttatg gcaaagggtt ggaatgttca gccccagctt ttctgcggca
     1261 atgagcaacg tggtgccagg gtcaacctgg gtagttatcc cgttgatttt taatgttatt
     1321 ttccctgatc tttgtattgt ctggtccttg tttttttcta catttgatga tggattttct
     1381 gccataactc ctgatattat gaagcttcaa agataagctt aagaatttat gtagttgttg
     1441 atctttttta taataacagg aatttaacgg taaggctgta attttttctt ttcagtgatt
     1501 acttattaga aaacctaaaa ttgtgaaacc tcttaaattg atcaacaatg gaaactattc
     1561 gttattttca tcagccaact attagtgaaa gcctcgggaa cggctggtat gttttgaaga
     1621 aacatttttg gtggcttctt ttggtggtta ttattaccag cattttcgac ggcccttcag
     1681 caaaatatac ttacgattca gatggttcgc tcctaaaggc aattggtgcc gcatcgctac
     1741 ttattgggtt tggtacgctt atatttttat tggtacgacc agttgtttct tggggtgcta
     1801 agcgcgtttt tcttcaatgt gcccggaatg aaaatcccaa cttaagctat ttatttgctg
     1861 gtttccagca caattacttc aatatagtgc ttacgaactt attactgacc attattattg
     1921 tggccggttt tctgttcctg attttacctg gaatatttct ggcgattcgg ttggttttcg
     1981 ttccttattt
//
LOCUS       DAOPUW010000367         1988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_61794_length_1988_cov_3.478531, whole genome shotgun sequence.
ACCESSION   DAOPUW010000367 DAOPUW010000000
VERSION     DAOPUW010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61794_length_1988_cov_3.478531"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PKJ43_06660"
     CDS             <1..364
                     /locus_tag="PKJ43_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72285.1"
                     /translation="QVEAATVGLMDAAANRGDTKPAKEIINSLKTKGLEFNRLHKQLQ
                     SAEHTADSLRLRYDYGVSQGTKDITYTIVVERPFPADKKSYPVRWLIVFLSTVATLLA
                     SIVTVVTIDYFRERSSAK"
     gene            342..1832
                     /locus_tag="PKJ43_06665"
     CDS             342..1832
                     /locus_tag="PKJ43_06665"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNZ72286.1"
                     /translation="MKEVQQNNNIIPDSWLKYILFYAISLLFIFINAYLVVKKDTLFA
                     VALPVLFAVLLTALYSYDKLIWIAILFAPLSVPLKAITSGLPIDMHLPTEPLIFGTLL
                     LFILSIAQGKRIGTNLTRHPVSIVLYFYLGWMLLTSITSTLPMVSFKYLLSRTWFIVV
                     FFFILAYLFEKEKNIEKFLWMFLIPLVPVMLFTIGRHLSYGLHDNQAAHWVMYPFFKD
                     HTSYGAVLAMCLPFLIGFSFSGWIKQKYKIWIWGLTGFYILAELLSYSRAAWLSLMAA
                     GGVWVVMKLKIKFKTIAITFVSLLLIVFAFQDQILQRLERNSQDSSANLTEHLTSMTN
                     ISTDASNLERLNRWHCAIKMFNEKPFFGWGPGTYAMNYAPFQKASMRTIISTNSGDGG
                     NAHSEYLGSMAESGFIGTLSFIAIIIVVLATGINAYSKTSDKRLKMLLISSILSLITY
                     YTHGILNNFLDMDKVSIPFWGFTAIIVAIDLQIKRQKNTRLEEQNG"
BASE COUNT          548 a          454 c          396 g          590 t
ORIGIN      
        1 acaagtagaa gctgccaccg ttggtctaat ggatgcagca gccaaccgtg gagataccaa
       61 accggccaaa gaaataatca acagcctaaa aaccaaggga cttgagttta accggttgca
      121 caaacaattg caatcggccg aacatactgc cgactcgctg agattacgct acgactacgg
      181 ggtaagtcag ggaacgaaag atataaccta cacaattgtg gttgaaaggc cattcccagc
      241 cgacaaaaaa tcgtacccag tgcgctggct catcgtgttc ttatcgaccg tggctacttt
      301 gcttgcatca attgttaccg ttgttacgat cgactatttc cgtgaaagaa gttcagcaaa
      361 ataacaacat catacccgat agttggctta agtacattct gttttacgcc atttcattgc
      421 tgtttatttt tataaacgca tacctggtcg taaaaaagga tacactgttt gccgttgccc
      481 ttccggtatt gtttgccgtg cttcttactg cgctgtattc atacgataaa ctcatctgga
      541 ttgccatact ttttgcacca ctctcggtac cgttaaaagc gatcaccagc gggttgccca
      601 tagatatgca cctgcccact gaaccactta tattcggtac actgttgtta tttatcctta
      661 gcattgccca aggaaaaaga attgggacca acctcacccg tcatccggtt tctattgtat
      721 tgtatttcta ccttggatgg atgttgctaa cctcgataac cagcacatta ccaatggttt
      781 ctttcaagta ccttctttcg agaacctggt ttatcgtggt gtttttcttt atcctggctt
      841 atcttttcga aaaagaaaaa aatattgaga aattcctttg gatgttttta atccccttgg
      901 taccggtaat gctatttacc attggccgtc atctttcgta tgggcttcac gacaaccagg
      961 cagcgcactg ggtaatgtac ccgtttttta aagaccacac ttcgtatgga gcagtgttgg
     1021 ccatgtgcct gccatttctc atcgggtttt ctttttcagg atggattaaa cagaaatata
     1081 aaatttggat ttgggggctg actggttttt acattcttgc cgaattgctc tcctattcgc
     1141 gggctgcatg gctcagttta atggccgccg gaggtgtttg ggtggtcatg aagcttaaaa
     1201 tcaaattcaa aaccattgcc ataacttttg tttcgttatt gttgattgtt ttcgccttcc
     1261 aggatcagat tcttcaacgg cttgagcgaa actctcaaga ttcatcagcg aatttgacag
     1321 agcaccttac ttcgatgacc aatatttcaa ctgatgcttc gaaccttgaa cggcttaacc
     1381 gctggcactg tgcgataaaa atgttcaacg aaaaaccttt ttttggctgg ggacccggta
     1441 cttatgccat gaattatgcc cctttccaaa aagcatcgat gcgtaccata atcagtacaa
     1501 actctggaga tggaggaaat gctcacagtg aatatctggg atcgatggcc gaatcgggtt
     1561 ttattggtac attgagtttt attgctatca ttatagtcgt gttagccacc ggcataaatg
     1621 cctattcaaa aacctcagac aagagactga aaatgttgct catttcatcg atactaagcc
     1681 taatcaccta ctatacccac gggatactaa acaactttct cgatatggat aaggtatcga
     1741 ttccattctg gggcttcaca gcaatcattg ttgccatcga tcttcaaata aaacggcaaa
     1801 aaaatacccg ccttgaagaa caaaacgggt aaattatctt tttttgaaaa acttatcaga
     1861 aagtatacct tatcccaaga gctgcacccc agcccaagcc tgcattatcg cccaaaaaat
     1921 cccacatcgg gcgggcatcg atactgagta aaattggagc tccttttttg ctgaaattgt
     1981 attcaagg
//
LOCUS       DAOPUW010000368         1988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_61815_length_1988_cov_2.275737, whole genome shotgun sequence.
ACCESSION   DAOPUW010000368 DAOPUW010000000
VERSION     DAOPUW010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61815_length_1988_cov_2.275737"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1678)
                     /locus_tag="PKJ43_06670"
     CDS             complement(50..1678)
                     /locus_tag="PKJ43_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72287.1"
                     /translation="MRTRLLTLFFVIVASTLVAQDIIKSKSIPTTYDRSSFAVFYIEN
                     PAQNHWSEVRPMIDSVIFSDKYDNNNFSNIIISSSIAGQLPTKGTQDVLLNELNNMNV
                     GRQLIAKWYNRQPDGSMNMELIHKRGRFTATDADFLKAQTSKRGNAALEDFGNRLINL
                     SHILVIDLQNVKTTEELEIKNMKGWQANAIGYLFRLDFNEEKQNELYDTWIYDDDTED
                     VKNQKRAAFDKIVFSLNPVTTQIVLISATQLESSSGIRLALKAKSMEQLLKELVQKSY
                     DEIIYKTEMKVEDLKVKTPLYQTRPLRAKIGLKEGLKTDYRFFVYEHVFNPKTNEAEP
                     KRRGVIRVAGRSSIVDNRKEATGDMGTSKFYQVAGRKLEAGFTLQQQNDFGLELIAGG
                     EAGNTSGLLLRADFRLGRFIGIPAFYLYGEFGALESKYYYGTEASFVRYGAGLAKGFQ
                     LGRNLEIRPYFGFGLDNTTDEGIGSTISSLYIKPGVNLALNLRHNIQLVGGLGAYSFI
                     GEVTDASGYTVAGSWDDLYDKSGPSALFGVKFMF"
BASE COUNT          597 a          427 c          354 g          610 t
ORIGIN      
        1 caaacgaatt gttttcatag taaaaaagat ttaatagtta gaaatgtatt tagaacataa
       61 atttcactcc gaataatgcc gaggggccgc ttttgtcata tagatcatcc cacgaaccag
      121 ctactgtgta cccgctagca tcggtaacct cccctataaa actataggca ccaagccctc
      181 cgacaagttg tatgttgtgt ctcaaattta atgccaagtt aactccgggc ttaatataca
      241 atgaactaat tgtactaccg attccttcat ctgttgtatt atcaagccca aatccgaaat
      301 atggacgtat ttccaaattt ctacccagtt ggaatccttt ggctaaacct gctccatacc
      361 tgacaaatga tgcttctgtg ccataataat attttgattc caatgcgcca aactctccat
      421 agaggtaaaa tgcaggtatt ccaatgaaac ggcccaagcg aaaatcggca cgcagtaaaa
      481 ggccggaagt attacccgct tcgccgcccg ctatcagttc caatccgaaa tcattttgct
      541 gctgcagagt gaagcctgcc tccagttttc ttcccgcaac ctgatagaat tttgatgttc
      601 ccatatcgcc tgtggcctcc ttcctgttgt caacaatgct tgatctccct gctacccgaa
      661 tgacacccct tcttttgggt tcggcctcgt tggttttcgg attgaaaaca tgttcgtaca
      721 caaagaaacg gtaatcggtt ttcagcccct ctttcagacc aattttagcc ctaagcgggc
      781 gggtttggta aaggggtgtt ttaaccttta gatcttctac tttcatttca gttttgtaga
      841 taatctcatc gtacgatttc tgtaccaact cttttaacag ttgctccata cttttcgctt
      901 tcagtgccaa acggataccg gaagaagatt caagttgagt tgcagaaatg agaacaattt
      961 gtgttgtaac cggattaaga gaaaaaacaa ttttatcgaa agcagcccgt ttctgatttt
     1021 taacatcctc cgtatcatca tcataaatcc aagtgtcgta aagttcattc tgtttttcct
     1081 cattgaaatc taggcgaaaa agatagccaa ttgcatttgc ttgccacccc ttcatgtttt
     1141 ttatttccaa ctcttctgtt gttttaacat tctgaaggtc gatgacaaga atatggctaa
     1201 ggttaatcag ccgattgcca aaatcttcga gggcggcgtt acctctctta cttgtttgag
     1261 ctttcaaaaa atctgcatca gtagcagtaa accgcccacg cttatgaatc aattccatgt
     1321 tcattgaccc atccggttgc ctgttatacc atttggcaat cagctgacgg ccaacattca
     1381 tgttatttag ctcattgagc aacacatctt gggtgccttt tgtaggaagt tgacctgcta
     1441 tagaggagct gattataatg ttactgaaat tattattatc gtacttatcg gaaaagataa
     1501 cagaatcgat cattggacgc acctcgctcc aatggttttg tgcgggattt tcaatgtaaa
     1561 aaaccgcaaa cgaactacga tcatacgtag taggaatgga tttagacttt ataatatcct
     1621 gagcaacaag tgtgctcgca acaattacga aaaaaagagt tagtaaacgt gttctcatag
     1681 tgattataat ttaagaggtt ttaagatatt atgtttctta aaaaaattag ccaaacaaaa
     1741 gtttcacaag ttaaattgcc aaaatgaaaa acaaaaagca ttgtttgcaa caataattat
     1801 gctaaaaatt aaaggtcaag aaccacccac ttttcacact ctgtctatgc gaagattaag
     1861 gagcaatttg aaatttctat gttacttctt caaatgaaaa attcaggtat aaagttagca
     1921 aaaaaatggg gaagtcaatc attattcgag aaatttccct tgcccggcat ttactcttcg
     1981 tccgagac
//
LOCUS       DAOPUW010000369         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_61947_length_1985_cov_3.507772, whole genome shotgun sequence.
ACCESSION   DAOPUW010000369 DAOPUW010000000
VERSION     DAOPUW010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61947_length_1985_cov_3.507772"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="PKJ43_06675"
     CDS             <1..850
                     /locus_tag="PKJ43_06675"
                     /inference="COORDINATES: protein motif:HMM:NF014999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF234 domain-containing protein"
                     /protein_id="HNZ72288.1"
                     /translation="PLFNRADNIIKLAAFDTETLKEIIRDYYPEYTNDDLLALYSFTG
                     GIPKYVELLCDGKRLDVNGMIEFMVRDNSPFTDEGKNLLIDELGKNYATYFSILSAIS
                     GGQNTQPEIEAALGNKGIGGQLKRLIEDYNLISRQRPILAKENTQTVRYEIHDCFLRF
                     WFNYFDRHRSLIEIKNFKALQEIIKTDYTTYSGKLLELYFKQKMAESKEYRMIGSWWE
                     AKKDQREIDIVGIKLEKNKAFAVEVKRQKKNFKPNIFDEKVQHLKQKVLPKYHIEPQC
                     WSLEDM"
     gene            complement(927..>1985)
                     /locus_tag="PKJ43_06680"
     CDS             complement(927..>1985)
                     /locus_tag="PKJ43_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HNZ72289.1"
                     /translation="NAFFAFTVVLGMGHSWQFALTAVFLEGLIFIALTAFNIREMIVN
                     SIPTNMKHAISVGIGLFIAFIGLKNAGIIVSNESTFVALGDVKNVAENPGALVALLGL
                     VITGALLALRVKGALLIGIIATAVIGIPFTVTKFPETFDLTPPSIAPVFMQFEWSNIF
                     TIDMLVVVFTFLFVDMFDTVGTLIGVSSKANMLDKEGRVPRVKQALMADAIGTTAGAI
                     LGTSTVTTYVESAAGVSEGGRTGLTSLTVAGMFLLALFLSPIFLMIPGAATAPALILV
                     GAMMMSPIKNIEFDDYTESIPVFLTIIMMPLTYSISEGILFGVLSFVILKLLSGRIKD
                     ISIVTAILAVLFLLKFLM"
BASE COUNT          682 a          415 c          393 g          495 t
ORIGIN      
        1 acctctgttc aaccgtgccg acaatatcat caaacttgcc gctttcgaca ccgaaacgtt
       61 gaaggaaatc atacgcgatt attaccctga atataccaac gatgatttgt tggcacttta
      121 ctcgtttacc ggcggaatac caaaatatgt ggaattgctt tgcgatggca aacggttgga
      181 cgtgaatggg atgatcgagt ttatggttcg agacaactcg ccgtttaccg acgaagggaa
      241 aaaccttttg atagacgaac ttgggaaaaa ctacgcgacc tatttctcaa ttctaagtgc
      301 catttcgggc ggacagaata cgcagcccga aattgaagcc gctttgggaa ataaaggcat
      361 aggcggtcaa ctgaaacggc tgattgagga ttacaacctt atttctcgcc agcggcccat
      421 tctggcaaaa gagaacactc aaactgttcg ctacgaaatt catgattgct ttcttcggtt
      481 ctggttcaat tatttcgaca ggcaccgttc attaatcgag ataaaaaatt tcaaagcctt
      541 acaagaaata attaaaacag actacacaac gtattcgggc aaattgcttg agttgtactt
      601 caagcaaaaa atggccgaga gcaaagaata ccgtatgatt ggttcgtggt gggaagccaa
      661 gaaagatcag cgcgaaattg atattgtggg cataaagctc gagaaaaata aagcttttgc
      721 agttgaagtg aaacgacaaa aaaagaactt caaacccaat atttttgatg aaaaagttca
      781 gcacttaaag caaaaggttt tacccaaata ccacatcgaa ccccaatgct ggtcgttgga
      841 agatatgtaa caaaaaaagg tgtctaaagt agaaaataac ctttcattct ttaagacacc
      901 tttttaatcg tttattataa aacttcttac atcaaaaatt tcaaaagaaa caacacagcc
      961 aaaattgcgg taacaatgct gatatcttta atccttcctg acaacaactt cagaatgaca
     1021 aatgaaagaa caccaaaaag aataccttca gaaattgagt aggttaaagg catcatgata
     1081 attgtaagga atactggaat cgattcggta taatcatcaa attcgatatt cttgattgga
     1141 gacatcatca ttgcaccaac caaaattaat gctggagcag tagctgcacc cggaatcatt
     1201 aaaaagattg gcgacaggaa caatgccaac aagaacatgc cggcaacagt tagcgaggtt
     1261 aagcctgtgc gccctccttc agaaactcca gcggcacttt caacataggt ggtaactgtt
     1321 gatgttccca atatagcgcc tgccgttgtt cctatagcat cggccataag agcctgtttt
     1381 acacgaggta cgcgaccttc tttgtcgagc atgtttgctt tcgatgaaac gccgataaga
     1441 gttccgacag tgtcgaacat atcaacgaac aaaaacgtaa agacaactac caacatatcg
     1501 atagtaaata tgttactcca ttcaaattgc ataaaaacag gagcaataga aggaggtgtt
     1561 aagtcgaatg tttctgggaa ctttgtaact gtaaacggaa taccaataac tgctgttgca
     1621 ataatgccaa taagcaatgc gcctttaacc cgtaatgcaa gcaaagcgcc agtaattacc
     1681 aggcctagca aagccactaa tgccccaggg ttctcggcca cgtttttaac atcgcccaaa
     1741 gctacaaagg ttgattcgtt gctaacaata atacctgcgt ttttcaatcc tataaatgca
     1801 atgaaaagac caattcccac cgaaattgca tgcttcatgt tggtggggat cgaattaaca
     1861 atcatttccc tgatattgaa cgctgtgaga gcaataaaaa tcaatccttc gagaaaaaca
     1921 gcggtaagtg caaactgcca ggagtgaccc ataccaagta caaccgtaaa tgcaaagaaa
     1981 gcatt
//
LOCUS       DAOPUW010000370         1983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_62063_length_1983_cov_3.133299, whole genome shotgun sequence.
ACCESSION   DAOPUW010000370 DAOPUW010000000
VERSION     DAOPUW010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62063_length_1983_cov_3.133299"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="PKJ43_06685"
     CDS             <1..1022
                     /locus_tag="PKJ43_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72290.1"
                     /translation="NIGFGIEATDYLDASWSKCGIFKLEIRVDSLLVNSFTIDELAYE
                     KSRQMNSHIDFEEFVSTKRRIQKTYIEPGNRLDIYSNIVNNGIVNISDGAKHKISISV
                     FDAAMNRSEIVFSVLATKPVAFPEKKYAAYFNFDRKNKFEAKDVELILPEEALYTDVK
                     FTYKRLPKTVGIYSDLHQLHDNTVPLNNYAEVKIKATRLPEHLRHKAIICLADAQGTP
                     KTSLGGEYSFGWIKTETRNLGTMCIAVDTIAPRIRPLSFSEQNTLTEKDRIRFKITDN
                     LSGIKTYNGFIDDTWVLFEFDAKSNTITYRFDEKIVRGKKHQLKVLIEDAKANKREYS
                     TSFYY"
     gene            complement(1274..1504)
                     /locus_tag="PKJ43_06690"
     CDS             complement(1274..1504)
                     /locus_tag="PKJ43_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72291.1"
                     /translation="MVVIRFTGWRVGMRPILFIKLLHVKAKLSLVESKRIKDRIVDEN
                     EIVEVKMNSEKNAIEILNEAKELGVIGELIIQ"
BASE COUNT          619 a          413 c          371 g          580 t
ORIGIN      
        1 gcaacattgg atttggaatt gaagccaccg attacctgga tgccagctgg agcaagtgcg
       61 gaattttcaa actcgaaata cgcgtcgact cgctattggt caattcattt actattgacg
      121 aactggctta cgaaaaatcg aggcaaatga acagccatat cgatttcgaa gaatttgttt
      181 cgacaaaacg ccgcattcag aaaacatata tcgaacccgg caaccgcctc gatatctatt
      241 caaacattgt gaacaacggg attgtgaaca tttccgacgg ggcaaaacac aaaatcagca
      301 tttcggtttt tgatgcggcc atgaaccgct ctgaaattgt tttttcggta ctggctacca
      361 aaccggttgc ttttcccgag aaaaaatatg cggcatattt caattttgac cgaaagaata
      421 aatttgaagc caaagatgtt gaacttattt taccagaaga agccttatat accgatgtga
      481 agtttaccta taaaagattg cctaaaacgg tgggcattta ttccgatctc catcagctac
      541 atgacaatac ggtaccgcta aacaattacg ctgaagtgaa aattaaagca acccgtttgc
      601 ccgaacattt gcgccacaaa gcgataattt gccttgccga tgcccaaggg acaccgaaaa
      661 caagccttgg gggtgaatat tctttcggct ggataaaaac tgaaacccgg aatctgggaa
      721 ctatgtgcat cgcggtagat accatcgccc caagaataag accattaagc ttttcggaac
      781 aaaataccct taccgagaaa gaccggatca ggtttaaaat taccgataac ctttcgggca
      841 ttaaaacgta caatggcttt atcgacgaca cgtgggtatt gtttgagttt gatgcaaaaa
      901 gcaacaccat tacataccgt tttgatgaaa agattgtcag ggggaaaaag catcagctaa
      961 aagtacttat tgaagatgcc aaggcaaaca aaagggaata ctcaaccagt ttctattatt
     1021 aacctgagct catttttttc cacacctctt cgagatcaag cacatagcga tgaaagtcga
     1081 tgctgccggt aacgaaacga tcaaacaaga tttgtgtcaa agttcagacg aataaaccag
     1141 ccccacggtt atggcaggct ggttgttata gtgcttcgtt gcgcaaaaga tcaggaatta
     1201 gtcggattgt aaatcggttg ttaaagaagt ctggattgcc atccgaacca gcaaagagta
     1261 tagctgcttt tatttattgt attattaact ctccaattac tcctaattct ttagcttcat
     1321 ttaggatttc tattgcattt ttttcactat tcattttaac ctcgacaatt tcattctcat
     1381 ctacaattct atcttttatt cttttagatt cgactaatga tagttttgct tttacatgta
     1441 aaagctttat aaatagaata ggacgcatgc ctactcgcca tcctgtaaat ctaattacta
     1501 ccatacttat ttgatgtcaa acactagtta tgaagagatt ataccaggat tagcaccagt
     1561 tgtccctgct ggtcgctatt tgcaatcccg acttcaatga ccaaggattt acaatccaaa
     1621 ttctatagtt atcaatccct ttcgcctgca tttctcttat tccagttgat tagtaatatt
     1681 taccagccag tactattttg tttttgatcc ccatgagttg taaggataat gaattagaag
     1741 ttaagcagga ttgcaattga aaatcctttg ttaatactca tccctaacaa caccatcggt
     1801 ttgaaaaaaa aacctccgga aatccgaagg cttatataat tgattgattc aatttctact
     1861 ttttcttttt tcttttcaaa aagattccgc cggcgatagc accaaatcca agaagagagg
     1921 ctattaacgg aatgggcact gcagtaccag ttcctgctaa gattgcctga tcgggagtcg
     1981 tgg
//
LOCUS       DAOPUW010000371         1983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_62066_length_1983_cov_2.956950, whole genome shotgun sequence.
ACCESSION   DAOPUW010000371 DAOPUW010000000
VERSION     DAOPUW010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62066_length_1983_cov_2.956950"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PKJ43_06695"
     CDS             complement(<1..629)
                     /locus_tag="PKJ43_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNZ72292.1"
                     /translation="MDLIQLTTGLNTFWVLITGALVFFMQAGFALVEAGFTRSKNTTN
                     ILFKNLMDFCIGTIVFWLLGYGIMFGAGNGFFGEIEWLSKVDHASALGIPNMAFFFFQ
                     LVFAATAATIVSGAMAERTKFSSYLIYSAVISLVIYPISGHWIWGGGWLASMGFHDFA
                     GSTVVHSVGGWLALVGAAVLGPRLGKYKNGKIYAIPGHSMTLGALGVFIL"
     gene            complement(651..998)
                     /locus_tag="PKJ43_06700"
     CDS             complement(651..998)
                     /locus_tag="PKJ43_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNZ72293.1"
                     /translation="MKKIEAIIRISQFEEVCEALHEVGIDFFTYWDVTGVGNEKAGAI
                     YRATVYETRLIPRRVITFVCRDQFVEAALDAICISARTGELGDGKIFISPIDDSVRIR
                     TGEKGPESLYIKD"
     gene            complement(1047..1694)
                     /locus_tag="PKJ43_06705"
     CDS             complement(1047..1694)
                     /locus_tag="PKJ43_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72294.1"
                     /translation="MKKIYALLVALVLTSVITSDLKAQFTAGVDLYSSYVWRGSKFAG
                     PSVQPSVKFTTGGLTAGVWGSYDFSGYKETDPYISYSLPIGLSIGVTDYYYEGDITDF
                     SDSTGSHALEVNLGYTIKGLSLAGNYIVNEAGGAASMGGDMYFQATYAFSSFSAFIGA
                     GNGWHTSDSEFNVCNIGIQASKSIELTDKFSIPVTGQVIVNPDKKQLFMVVGLSF"
BASE COUNT          592 a          428 c          366 g          597 t
ORIGIN      
        1 agaatgaata cgccaagagc gcctaaagtc atgctgtggc cagggatggc ataaatttta
       61 ccatttttat atttgcctag tcgaggtccg agaactgctg caccgactaa agccagccat
      121 cctccaacag agtgtactac tgttgaacct gcaaaatcgt ggaagcccat tgatgccaac
      181 catccaccgc cccaaatcca gtgtcccgaa atagggtaaa ttaccaatga tataacggca
      241 ctataaatta ggtatgaact gaattttgtt cgttcggcca ttgctcctga aacaatggta
      301 gctgcagttg ctgcaaatac cagctggaag aagaagaatg ccatgtttgg aatgcctaaa
      361 gcagaagcat gatcgacctt agaaagccat tcaatttctc caaagaaacc attgcctgcc
      421 ccaaacatga taccgtaacc caacaaccag aaaacaattg ttccgataca gaagtccatc
      481 aagtttttga acaagatgtt tgtagtattc ttagaacgag tgaaacctgc ttcaactaat
      541 gcaaaaccag cttgcatgaa aaataccaga gcccctgtaa tgagaaccca aaatgtattt
      601 agacctgtcg ttaattgtat taaatccata attgtaattt tttatcgttc tcaatcttta
      661 atgtataatg attctgggcc tttttctcca gttctgatcc gaactgaatc atcgataggt
      721 gaaatgaaga tcttcccatc gcccaactct ccagttcttg ccgatatgca aatagcatca
      781 agcgctgctt caacaaactg atcgcggcat acgaaggtga taacccgtcg aggaatcagc
      841 cttgtttcat aaacagtagc acggtaaata gctcctgcct tttcgttgcc aacacctgtt
      901 acatcccagt aggtaaagaa gtcgatgcca acttcatgca aagcttcaca tacctcttca
      961 aactgggaga tcctgataat tgcttcaatt tttttcatct cttatttctt tataattcga
     1021 cattatcttt aatcaccgtt cggaaactag aacgataatc ctactaccat gaatagttgc
     1081 tttttgtcag ggttgacaat cacctggcca gttaccggga ttgagaactt gtcggtcaac
     1141 tcgatcgatt ttgatgcttg gatgccaatg ttgcatacat tgaattcgct gtcggaagtg
     1201 tgccatccgt tacctgcgcc aatgaatgcg ctgaatgaac taaatgcgta ggttgcctgg
     1261 aagtacatgt cgcctcccat gcttgctgca cctccagctt catttacaat gtagttgcct
     1321 gcgagggaga ggcctttgat ggtgtagccc agatttactt ctaaagcgtg gctgccggtt
     1381 gagtctgaaa agtcggtaat gtcgccttcg taatagtaat cggtaacgcc aatacttagt
     1441 ccgatcggta acgaatacga aatgtaagga tcagtctctt tgtagccact aaagtcgtac
     1501 gaaccccaaa ccccggctgt taagcctcct gtagtgaact ttaccgatgg ttgtaccgat
     1561 gggccggcaa atttactacc cctccataca taactactgt aaagatctac ccctgcagtg
     1621 aattgggctt tcaggtcact tgttattact gatgttaaaa caagtgcgac gagtaatgcg
     1681 taaattttct tcataacaat tcatttttta ggttatacat gcttgtctac ttctttttta
     1741 tcgaaaaatg gttttacctt ttctcttttt ggatgtttgt ttcctaatcc tgatccaaaa
     1801 gtaaattttg aatttatcga gacaaattat tttaagggta ttttcttaaa aaaaaatata
     1861 tattttaaaa taaacccctt taaaaatgac tcatatctcc ttaaaaagta ttattttttt
     1921 gaaataacca taaaaaacaa gggttaaatt gaataaaata tgttttttaa aatgtgtttc
     1981 aat
//
LOCUS       DAOPUW010000372         1981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_62151_length_1981_cov_3.575805, whole genome shotgun sequence.
ACCESSION   DAOPUW010000372 DAOPUW010000000
VERSION     DAOPUW010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62151_length_1981_cov_3.575805"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PKJ43_06710"
     CDS             <1..248
                     /locus_tag="PKJ43_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNZ72295.1"
                     /translation="PMCIPVYAAMTELKEEYKNVRFADFEFDNPEAAVIRDAPEAIGF
                     MGLPFTFYYKNGKAVAATSSIQSKDQIAAILDKHFTA"
     gene            269..757
                     /locus_tag="PKJ43_06715"
     CDS             269..757
                     /locus_tag="PKJ43_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNZ72296.1"
                     /translation="MKSFKIVPLIVLFSLLVSMLYAVDNQKNNSSSSASEVVVKNITK
                     SDFLKLVMNYEKNTKEWKFEGTKPCIIDFYADWCGPCRITSPILEELAAEYKGKIDIY
                     KVNVDKERELAQVFGISGIPAFLYCPLNGKPSMTSGIAKDKEQTKKMFIDNIESILLK
                     SK"
     gene            1049..1615
                     /locus_tag="PKJ43_06720"
     CDS             1049..1615
                     /locus_tag="PKJ43_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HNZ72297.1"
                     /translation="MGFLSLIFLAIGLSVDSFAASVCSGLSLCRKHLKIRQALKISLT
                     LAVFQGAMPALGWWLGSAFKGYIERADHWIALALLSVLGIRMIFESRVPLKERKVKNP
                     TSMKAIIPMAIGTSIDALAVGISLSFFVHEILFPSLLIGAVTLIFSLGGIYMGRKAGI
                     RLAGKAEIAGGLILILIGVKIFAEHMFF"
     gene            complement(1622..>1981)
                     /locus_tag="PKJ43_06725"
     CDS             complement(1622..>1981)
                     /locus_tag="PKJ43_06725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ72298.1"
                     /translation="SDDYYLKKAILRLIDNALKFTEKGYVTFEANMVDGCLEFSISDT
                     GIGIPEKDLIKIFEPFVQGDGSFTRNHGGAGIGLTIVKGITQALGASLDLETRLGAGT
                     RFSIKFEPYAVKDTGIQ"
BASE COUNT          550 a          351 c          415 g          665 t
ORIGIN      
        1 ggccaatgtg cattcctgtt tatgctgcta tgacagaatt gaaagaggaa tacaaaaatg
       61 tacggtttgc agattttgaa ttcgataatc ctgaggcagc tgtgattcgt gacgctcctg
      121 aagccatagg ttttatgggc ttgcccttta ctttttacta caaaaacggg aaagcagttg
      181 ccgctaccag tagcattcaa tcgaaagatc aaattgctgc tattcttgac aaacatttta
      241 ctgcttaatt acttaactca aaattgttat gaaatcgttt aaaattgttc cgcttattgt
      301 tttgttctct cttttggtgt caatgttata tgctgttgat aatcagaaaa acaattcatc
      361 ttcgagcgct tcggaggtag tggtcaaaaa tattaccaaa tccgattttc ttaaattggt
      421 tatgaactat gagaaaaaca caaaggaatg gaaatttgaa ggtacaaagc cttgtattat
      481 tgatttctat gccgattggt gtgggccatg ccgaataaca tctcctattc tagaagaatt
      541 ggccgccgaa tacaaaggga aaatcgacat ctataaagtc aatgtagata aggaacgaga
      601 gcttgcacag gtttttggaa tttcgggaat cccggccttc ttgtattgtc cgctcaacgg
      661 aaaaccgtca atgacttcgg gcattgcaaa agataaggag caaaccaaga aaatgtttat
      721 cgacaatatt gaatcaatac tgttaaaatc gaaataagct tttattttaa ttttgacaac
      781 aatgcaacgc tgaaggtttt ttcccttcgg cgttgcattt ttagtgttta tcacacatct
      841 gttatttcgt ttcggattct cggttgcatt taataacaga tgtaattata aatcagtccg
      901 aaccttttaa tgagtttttc ttgagtgaaa ggttacgctt ttttgtggca aattgtatcg
      961 aaatgttttg caaattgcga gctcaaattt tcatcagatg aaaccatgca tagattagtt
     1021 ttggttttgg gatttttaaa cagaaattat gggatttctt agcttaattt ttttggctat
     1081 cgggttatca gtcgattcgt ttgcagcatc ggtttgcagc ggattatcgt tatgtcgcaa
     1141 gcatttgaag attaggcagg cgctgaagat aagccttaca ctagctgtgt ttcagggggc
     1201 aatgccagcg ttgggttggt ggctaggttc ggccttcaaa gggtatatcg aaagagccga
     1261 tcattggatt gcccttgctc tacttagtgt tcttggaata agaatgatat ttgaaagtcg
     1321 cgttccgtta aaagaacgga aagttaaaaa tcctacatca atgaaggcca ttatccctat
     1381 ggctattgga accagtatag atgcattggc tgtcgggatt agcttgtctt tctttgtaca
     1441 tgaaattctt tttccatcat tgctgattgg tgcggttact ttaatttttt cccttggggg
     1501 tatctacatg ggacgaaaag caggtatccg tcttgccgga aaagctgaaa ttgctggtgg
     1561 tttaatttta attttaattg gtgtgaaaat tttcgccgaa catatgttct tttagtccac
     1621 tttattggat gccagtatct tttactgcat aaggttcaaa cttgattgaa aatcgggttc
     1681 ctgcgcctaa tcttgtttcg aggtcaagtg aggcaccaag agcctgtgtt attcctttta
     1741 caattgttaa accgatgcct gctcctccat ggtttctggt gaatgagccg tcgccctgaa
     1801 caaacggttc aaaaatcttt atcagatctt tttcaggtat cccaatcccg gtatccgata
     1861 tcgagaattc gagacaacca tcaaccatgt tagcctcaaa tgttacatag cctttttcgg
     1921 taaattttaa cgcgttatcg ataagtcgca gaattgcttt tttaaggtag taatcgtcgc
     1981 t
//
LOCUS       DAOPUW010000373         1981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_62159_length_1981_cov_2.828660, whole genome shotgun sequence.
ACCESSION   DAOPUW010000373 DAOPUW010000000
VERSION     DAOPUW010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62159_length_1981_cov_2.828660"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PKJ43_06730"
     CDS             <1..540
                     /locus_tag="PKJ43_06730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ72299.1"
                     /translation="PVNNRTLSGTECFNAQNDITVAGTSDVVIQDDATATFIAGHSIR
                     FLPGFHAQQGSYVDAHITTTGSFCDDLPAPIMAAEPVVEKSATEPIADENENQPSSLN
                     ACMKVYPNPNNGQFTVSFDNFDNETKVYVYNTFGQKVYQTSTWEKFTVVELQHVRRGI
                     YFVKAINNQRHYEQKIIVQ"
     gene            complement(604..747)
                     /locus_tag="PKJ43_06735"
                     /pseudo
     CDS             complement(604..747)
                     /locus_tag="PKJ43_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071135758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(722..895)
                     /locus_tag="PKJ43_06740"
     CDS             complement(722..895)
                     /locus_tag="PKJ43_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72300.1"
                     /translation="MDFPNAKFIKALYSDEEVNRMELEFQEKGYTILRNVFERESVKE
                     YKAQLQEAAARVS"
     gene            1114..>1981
                     /locus_tag="PKJ43_06745"
     CDS             1114..>1981
                     /locus_tag="PKJ43_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72301.1"
                     /translation="MNLIPSDYHNWLPSRLFEKDGALFLEWTYLCDVRFDMPFFDESL
                     AKCRTQNSMKGSDEKKIRITPINFLSEVAQTVDAIEPSLFIFHTSRCGSTLTSQVLTI
                     DSRNIVFPEYVIVDSILRSTINNKPVSVEKRKEWLNDLIKIMGHKRFPTEERLIIKLD
                     SWHFIYYNLFRELYPKLPFAILYREPEAILRSNNKQWGMQFIPELVSPSLFGIELDIS
                     STFSLNGYANLVLQSMYAIIFTIARTDNNSLLLDYSNGIEENLRRLTEILHIDHLFLQ
                     RKDVRERLKFHSK"
BASE COUNT          614 a          410 c          352 g          605 t
ORIGIN      
        1 ccggtaaaca acagaacatt atcaggcacg gaatgtttca atgcccaaaa tgacattacc
       61 gttgccggta ctagtgatgt ggtgattcaa gatgatgcta cggctacttt catagccggc
      121 cactccatcc gcttcctgcc gggcttccat gcgcaacaag gcagttatgt tgatgctcac
      181 attaccacta ccggctcttt ctgcgacgat ttgcctgccc cgataatggc tgccgaacct
      241 gtggtggaaa aaagcgctac cgaacctatt gctgatgaaa atgaaaatca gccaagtagc
      301 ctgaatgcat gcatgaaggt ttaccccaat cccaacaacg gacagtttac cgtttccttc
      361 gacaatttcg acaacgaaac caaggtgtat gtatacaata ccttcggaca aaaagtgtat
      421 caaacaagta cctgggaaaa attcacggtt gttgagttac agcatgtgcg gcgcggaatc
      481 tacttcgtga aggccatcaa taaccagcgg cactacgaac aaaagattat cgttcaataa
      541 cgaacagtag aaataagaaa tggcggctat aacaggtcgc catttttgtt tcagtttagg
      601 aggttagaat gctgctatga aattgatgtt atttaatctt ttaggttcag atagaaccat
      661 atttcttaga agtaacgaag atcccatcat tgatagtctg ttataccgaa aatagtactt
      721 gtcaagatac ccttgcagcc gcttcttgta gttgagcttt atactccttt accgattcgc
      781 gttcaaaaac attgcgtaaa atggtgtagc ctttctcttg gaactctaac tccattctat
      841 tcacttcttc atcggaatat aatgccttta taaattttgc attggggaaa tccatgatct
      901 gctttacgat ttaaaagcat tgaattattt cttcgtgtaa actaaataaa attatcaatt
      961 attctcttta aaatatgtat ctgaaccact ctaaagttac ctcctttcag aattactcca
     1021 tcttgaccat ttaaaataaa tttcaaaatt cgttgtatat tgcaataagt ataaaacaaa
     1081 atatacaagc caatagatac aatcctaatg ccaatgaatt taatcccatc cgattatcat
     1141 aattggctac catcaaggct tttcgaaaaa gatggggcgc tttttttgga atggacatat
     1201 ttatgtgatg tccgttttga catgcctttt ttcgatgagt cgttggccaa atgccgtacc
     1261 caaaattcga tgaaaggaag cgatgagaaa aagatcagaa ttacaccaat taatttttta
     1321 tcggaagttg cccagactgt cgatgctatt gaacctagtc tgttcatatt tcatacctct
     1381 cgatgcggat ctacccttac atctcaggta ttgactattg atagtcggaa tattgttttt
     1441 ccggagtacg taatcgttga ttctattttg cgatcaacaa ttaataataa accagtttcg
     1501 gtagagaagc gcaaagaatg gctcaacgat ttgataaaaa taatggggca taaacgtttt
     1561 cccaccgaag aacgtttgat tataaagctc gacagttggc acttcattta ttataactta
     1621 ttccgggagc tctatcctaa actacccttc gcaattcttt atcgtgaacc cgaagccatt
     1681 ctccgttcaa acaacaaaca atggggaatg cagttcattc ctgaattagt ttcaccttca
     1741 ctatttggca ttgaattaga tatatcttca actttttctt tgaatggata cgcaaatctg
     1801 gtgctacaat cgatgtatgc tatcattttt acaattgccc gtaccgacaa caatagcttg
     1861 ttactcgact atagtaacgg aattgaagaa aaccttcgcc gattaactga aattttgcat
     1921 atcgaccact tattcttaca acgaaaagat gttcgtgaaa gattaaagtt ccattcaaaa
     1981 c
//
LOCUS       DAOPUW010000374         1970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_62679_length_1970_cov_3.456919, whole genome shotgun sequence.
ACCESSION   DAOPUW010000374 DAOPUW010000000
VERSION     DAOPUW010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62679_length_1970_cov_3.456919"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..1737)
                     /locus_tag="PKJ43_06750"
     CDS             complement(202..1737)
                     /locus_tag="PKJ43_06750"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HNZ72302.1"
                     /translation="MKKVLTLIFLCLGMSCNSYAQSALKVELSVDLSSEPLEYNQMIF
                     GQFLEHFHRQIYGGIYEPGSKLSDEQGFRKDVIDALRELKVPVVRYPGGCFVSTYHWK
                     KGVGKNRIPVYDKSWHVEDPNTFGTDEFIAWCRKIGAEPFICTNAGTGTPEEMSDWVE
                     YCNLNIGEYGRMRIANGYPEPYNVKYWSIGNENYGAWELGAKTVDEWGYLVRESAKLM
                     LSVDQSIKLFAAATTDENWTVPLLERAGPYLSYISIHGYWDFMVEVNKPADYLTCILR
                     TTYPEEQIQRTIEILEKTGYDKRIKIAFDEWNLKNWHHPGHGRHLQGMDIKARDGNDV
                     NSLYTMADALFSACFLNSCLRNSNYVEMACMSPVVNTRGPLYVHPGGIVKRTTYHVMQ
                     MYANELQPYVLPVSIESELLTVKDRSVPAVDAVVTCSEDKKQLALALVNRDPQKEAEC
                     SISIADINNVAEAIVLSGDSTEAYNDIDNPNRVVPKIEKLRIKNGKITIPAHSLLIVK
                     LTR"
BASE COUNT          539 a          437 c          386 g          608 t
ORIGIN      
        1 aaaaatgcta taactaagaa ataaaatgag gtttttagta gagttttcat tgtatacgtt
       61 ttatgttgtt aataaatagg tgatgatttg taggttttga tgatggtttg tacagcttgc
      121 caaactgctg tctgaactct tattggcaga cgccgtctgt taaccgctgt cgttgccggt
      181 tgctggctgt tgtcgtctgt ttcaccgggt caactttact attagcaatg aatgggcagg
      241 aatggtaatc ttgccgtttt taattctaag cttttcgatt ttgggaacga ctctgtttgg
      301 attgtcgatg tcgttatagg cttcggtaga atcgccggaa agtactattg cctctgccac
      361 attattaata tctgcgatgc ttattgagca ttccgcctct ttctggggat ctctgttcac
      421 aagcgcaagg gccagttgtt ttttatcttc gctgcacgtt acaaccgcat caacagccgg
      481 aacagatctg tctttaacgg taagtaattc cgattcgatt gacaccggca aaacataggg
      541 ttgcaactcg tttgcataca tctgcatcac atggtaagtg gttcttttta ctataccacc
      601 ggggtgaaca tacaaagggc cccgcgtatt tacaaccggc gacatacaag ccatctccac
      661 ataatttgaa ttacgtaaac acgaattcaa aaaacaggcc gaaaaaaggg catcagccat
      721 agtataaagt gagttgacat cgttaccgtc tctagcttta atatccatac cttgtaaatg
      781 ccggccgtgc ccgggatgat gccaattttt caaattccat tcatcgaaag caatttttat
      841 tcgcttgtcg taaccggttt tttctaaaat ttcgatggtt cgttgaattt gctcttcggg
      901 atatgttgtt ctcaagatgc acgtaagata atcagcgggt ttgtttacct ccaccataaa
      961 gtcccaataa ccgtgtatcg aaatataact caaatacggt cccgcccgtt ccagaagcgg
     1021 aacagtccaa ttctcatcgg tcgttgcagc cgcaaaaagc ttgatcgact gatcgacact
     1081 gagcatcaat ttagcggatt ctcttaccaa ataaccccat tcatcaacgg ttttagcgcc
     1141 caattcccag gccccatagt tctcattacc aatactccaa tacttcacat tatagggttc
     1201 cggataaccg ttggctatcc gcatcctgcc gtattcacca atattcagat tgcagtattc
     1261 aacccaatcg ctcatttctt cgggcgtccc cgttcccgca ttggtacaaa taaagggctc
     1321 agcacctatc tttctacacc aggcaataaa ttcatccgtg ccaaaagtat ttgggtcttc
     1381 cacatgccag gatttatcgt aaacagggat tctgtttttc cccactcctt ttttccaatg
     1441 gtaggtcgac acaaaacatc ccccggggta tcgaacaacc ggaactttaa gttcacgtaa
     1501 ggcatcaatt acatctttac gaaacccctg ttcgtcagaa agttttgaac cgggctcgta
     1561 aatcccgcca tatatttgcc ggtgaaaatg ttccagaaat tgaccaaata tcatttggtt
     1621 atactccaaa ggttcggaac tcaaatccac agacagttca accttcaatg cgctttgagc
     1681 ataagaattg caggacattc ccagacaaag gaaaataagc gttagtactt ttttcatggg
     1741 tggttggttt ttggttttca ttaagaatga aaacttaaat gatgtattgg aatattttga
     1801 caatatggcc ggtaagagac cgcgggcttt tttctatcat aatgaacaat aagttgaagc
     1861 agattgcacc caattgaata acctacacgt atttgtttcc attgatatta cccgctttag
     1921 tttttattct cgcttagtat agatgaaaaa agtggcgatt tgttacttga
//
LOCUS       DAOPUW010000375         1969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_62731_length_1969_cov_3.512017, whole genome shotgun sequence.
ACCESSION   DAOPUW010000375 DAOPUW010000000
VERSION     DAOPUW010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62731_length_1969_cov_3.512017"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PKJ43_06755"
     CDS             complement(<1..600)
                     /locus_tag="PKJ43_06755"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HNZ72303.1"
                     /translation="MGTSSTNDKISGAEIHFAPMQGFTDMAYYSAFSKHFAGVGYYYT
                     PFFCVDDRLERTFFAGSDLVLEKLVWQILPKDIDEMKVLTHFVLRQGGSMLNINLGCP
                     YPMVTRRGRGAALISNPEMVVSMIDYIRNYTPLTVSIKTRLGLSRDDEVFPLLEALAR
                     KSCTQVIVHARTATQLYKGNVNTERFLECRLRFPEIDFVY"
     gene            662..1528
                     /locus_tag="PKJ43_06760"
     CDS             662..1528
                     /locus_tag="PKJ43_06760"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HNZ72304.1"
                     /translation="MSNQIMEQQVDFSVVSDYIVNPDTSAVSLARDSVYLFYQEDVLS
                     ASDSLYQELINPLTIKIKPSGEYGFIPKAKSDFIPNWTIILIIILFAILAAIRSASEN
                     YVVQLFQSLFDRKSATKLFKEKVSTLMSVTFRLDTFFILTTGSFIYQLADHFIGFDDN
                     ERFLYLGLCIVALFLYINLKYSLYLLSGAIFDTKDETREYIFHAKTGNRIMAIILFPI
                     VLSLFIAQGEFAEFLVFFGLGIVAIMLVIKTLRSMLLIAQKVFSFYYLILYLCTLEIL
                     PLLLMGKFLMNV"
     gene            1558..>1969
                     /locus_tag="PKJ43_06765"
     CDS             1558..>1969
                     /locus_tag="PKJ43_06765"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNZ72305.1"
                     /translation="MKIKRILVSQPEPTSVKSPYHEFAERNNVKVDFRSFTEVEGVSA
                     KEYRQTRVSILDHTAVIFTGRTAIDHFFRICADLRIVVPETMKYFCMSEATAFYLQKY
                     IVYRKRKIFYSDGPFSDLLEIMKKHLDETYLLPLS"
BASE COUNT          612 a          408 c          357 g          592 t
ORIGIN      
        1 gtatacaaaa tcgatttcgg ggaaacgaag cctgcactcg agaaaccttt ctgtattgac
       61 gttgccttta tacaattggg ttgccgttcg ggcatgaact attacttgag tacaactttt
      121 tctggccagg gcttcgagaa gtggaaagac ctcatcatca cgagaaagtc ccaaacgggt
      181 tttaatgctt acagttaatg gggtataatt acgaatataa tcaatcatcg acaccaccat
      241 ttcaggattg ctgataaggg cagcccccct accccggcgt gtaaccattg gatatggaca
      301 gcccagatta atgttcaaca ttgaaccacc ttgccgcaga acaaaatgtg tcagcacttt
      361 catctcatca atatctttgg gcaaaatctg ccataccaat ttttcgagta caagatcaga
      421 gcctgcaaaa aaagttcttt ctaaccgatc atcgacacaa aaaaacggag tataataata
      481 ccccacccct gcaaagtgtt tagaaaaagc tgaataataa gccatatcgg taaaaccctg
      541 catgggcgca aaatgaattt cagccccgct aattttgtcg ttagtcgatg atgttcccat
      601 agaatgcaaa aataaaaaac tatatttgcc cgcaaatcaa agtagcaaaa ttgaatcatc
      661 cgtgtcgaat cagattatgg aacaacaggt tgattttagt gtggttagcg actatattgt
      721 caatcccgac acctcggcag taagcctggc acgtgattct gtttacctct tttaccaaga
      781 agacgtacta agtgcttctg attcccttta tcaggagctc ataaacccgt taaccataaa
      841 aattaaaccg tccggagaat acggtttcat cccaaaagca aaatccgact tcattcctaa
      901 ctggacaatt atattaataa tcattctttt cgccatactg gctgcaatac gatccgcttc
      961 ggaaaactat gtggttcaat tgtttcaatc gctgtttgac cgcaaatcgg caacgaaact
     1021 tttcaaagaa aaagtaagca cattgatgag cgtcaccttc cgtctcgata ctttttttat
     1081 actcacaaca ggttctttca tatatcaact tgccgaccat tttattggat ttgacgacaa
     1141 tgagagattc ctatatttgg ggttatgcat tgtagccctg ttcttatata tcaatctgaa
     1201 atattcgctt tacctattaa gcggggctat ttttgacacg aaagatgaaa cacgagaata
     1261 catatttcat gcgaaaacag gaaaccgcat tatggccatt atcttgtttc ccattgtact
     1321 ttctttgttt attgcacaag gcgaatttgc cgaattctta gttttctttg gcctcggaat
     1381 agtagctata atgctagtta taaaaacatt aagaagcatg ttgttaattg ctcaaaaagt
     1441 tttttcattt tattatttga ttttatacct ttgtaccctc gaaattttgc ccctcttgtt
     1501 gatgggtaaa tttttgatga atgtataaaa aattgtgaaa tcataaaaac ttaggttttg
     1561 aaaatcaaga gaatacttgt ttcgcaaccg gaaccaacct cggtaaaatc accctatcac
     1621 gaatttgcag aaaggaacaa cgtaaaggtt gactttaggt catttactga agttgaaggt
     1681 gtatctgcta aagaatatcg tcaaacaagg gtaagcatac tcgatcatac ggcagtaatt
     1741 tttacaggcc gtacagccat cgatcatttt ttcaggattt gcgccgattt gcgaatagta
     1801 gtgcctgaaa ctatgaagta tttctgtatg tcggaagcaa ctgccttcta tttgcagaaa
     1861 tatatagtat accgcaaaag aaagatcttc tatagcgatg gccccttctc cgatttgctg
     1921 gaaataatga aaaagcatct cgacgagacc tatcttttac ccctttcgc
//
LOCUS       DAOPUW010000376         1961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_63113_length_1961_cov_3.416055, whole genome shotgun sequence.
ACCESSION   DAOPUW010000376 DAOPUW010000000
VERSION     DAOPUW010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63113_length_1961_cov_3.416055"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="PKJ43_06770"
     CDS             complement(<1..1074)
                     /locus_tag="PKJ43_06770"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNZ72306.1"
                     /translation="MKKNRSFLMFIIMILFSVTAVAQVTTSAISGKVTSDGKSPLPGA
                     TVVAVHQPSGSQYATIANSEGYFNLQGMRPGGPYNVKVSFVGYSSLSYTDITLSLGES
                     FLLNATLKEDVTEVSEVMVVGAKPSKFSAEKTGATTNISREQMSFMPTINRSISDIAR
                     VSPYANGMSFAGGDGRSTNFTVDGANFNNNFGLSSNLPGGGNPISMDAIEEVQVVIAP
                     FDVRQTNFIGGGINAITKSGTNQFKGTVYTYQRNQDMRGNKIGSYDFGTRAKESKSVY
                     GATLGGPIIKDKLFFFANFEYEKEPKQVIKWKASTDGVGDGQTISRTTEADLQRVSDH
                     LKSKYGYETGSYKDFPADMTNFKI"
     gene            1388..>1961
                     /locus_tag="PKJ43_06775"
     CDS             1388..>1961
                     /locus_tag="PKJ43_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNZ72307.1"
                     /translation="MHKPFLALAIAFILLFSTNESFSQSVQSQAMKLNWLLRTIDSYY
                     SDSVNIQDITEYAIVEMLSKLDPHSVYISEKEVQEMNEPLQGSFDGIGISFNIFHDTL
                     MVLQTIPGGPSERVGLAAGDRIIYVDNELIAGVGITNQDVFKKLKGEKGTLVKVRILR
                     KGDSELLDFNIIRDKIPLHSLDASRLCKGIL"
BASE COUNT          583 a          376 c          377 g          625 t
ORIGIN      
        1 gattttgaag ttggtcatat cggcagggaa atctttataa gaaccagttt catatccata
       61 tttcgatttg agatgatctg aaactctctg aagatcagct tctgtagtgc gggagatggt
      121 ttgtccgtcg cctactccat cggtcgaagc tttccattta attacctgtt taggttcttt
      181 ttcgtattca aaattagcaa agaagaacaa cttatctttg ataatcgggc cacccaaggt
      241 cgcaccatac accgattttg attcttttgc ccttgtgccg aagtcgtaac tgccaatttt
      301 gtttccgcgc atatcttgat tacgctgata ggtgtaaaca gttcctttaa attggtttgt
      361 tccagacttt gtaatcgcat tgattccacc tccaataaaa ttagtttggc gaacatcgaa
      421 aggagcaatt accacttgta cttcttcgat agcatccatt gaaatagggt tgccaccacc
      481 tggcaagttc gacgacaaac cgaagttgtt gttgaaatta gcgccatcaa cggtgaagtt
      541 tgttgaacgt ccatcgccac cggcaaaact cattccgttg gcataaggcg aaacccgtgc
      601 aatatctgag atactgcggt taatggttgg cataaacgac atctgttcac gcgagatgtt
      661 tgttgtagcc cctgtttttt cggcactgaa cttcgatggt ttagcgccaa ctaccataac
      721 ttcggaaact tcagtaacat cttcttttaa tgtcgcgtta agaaggaacg attcaccaag
      781 agatagcgta atatcggtat aactcagaga gctatagcca acaaaactaa ccttcacatt
      841 gtaaggtcca ccaggacgca tcccctgaag gttaaaatac ccttcagaat tggcaatggt
      901 tgcatactga gaacccgagg gctgatgcac tgcaactaca gtggcaccag gcaggggaga
      961 tttgccgtcg gaggtaacct ttcccgaaat tgcagaagta gtaacctgag caacagctgt
     1021 tacagaaaat aaaatcatga tgataaacat cagaaatgat cgattttttt tcataggatt
     1081 aataattaat tttattgtat cgtgtaattt ttttcgattt cgacttgttg tttttgattt
     1141 gattgcaaat aaacacaaca aaatgttaaa aaacattaag taaacctcaa aaaaatgttt
     1201 ccattttgtt tttcattttc ttttatgcgg aagcaattta gctattcggc aacgaattaa
     1261 caacgaaaaa ccactgcttg ttagtaagtt tttataaact ttcttacctc tttttcttca
     1321 agttttaatt tggatacttt tttaaaactt atatttgttt ttcgatacta ttaaagggga
     1381 gataattatg cataaaccat ttcttgcgtt ggcaatagca ttcattctat tgttttcaac
     1441 aaatgaaagt ttttctcaga gtgttcaatc gcaagccatg aagctcaatt ggctcttgcg
     1501 tactatcgat agttattatt ccgattctgt taatattcaa gatattaccg aatatgcaat
     1561 agtcgaaatg ctctcaaaac tcgatccaca ctcagtgtat atttctgaaa aagaagtaca
     1621 ggaaatgaat gaacccctac aaggaagttt cgacgggatc ggtatttctt ttaatatttt
     1681 tcacgataca cttatggtgt tgcaaactat tcccggaggt ccatcggaaa gagttggatt
     1741 agctgctggc gaccgtatta tttatgtcga caatgaactt attgctggtg taggaattac
     1801 caatcaggat gttttcaaaa aactcaaagg agaaaaaggt actttggtta aagtcagaat
     1861 attacgtaag ggtgattctg aactgctcga ttttaatatc atcagagaca aaatcccttt
     1921 gcacagcctc gatgcatcga ggctgtgcaa agggattttg t
//
LOCUS       DAOPUW010000377         1961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_63116_length_1961_cov_3.301154, whole genome shotgun sequence.
ACCESSION   DAOPUW010000377 DAOPUW010000000
VERSION     DAOPUW010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63116_length_1961_cov_3.301154"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PKJ43_06780"
     CDS             complement(<1..706)
                     /locus_tag="PKJ43_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72308.1"
                     /translation="MLILLSWMIVVSCEKEKYLVTGDAKLAFSTDTVMFDTIFTTFGS
                     TTQAFKVINPSVNPVEISSIVLAGGDQSFYRLNIDGEMVNKAQNISLRGQDSIYIFVE
                     VTIDPTGVDQPMVVKDSVVFTVGGVSQDVDLVAYGQDVILLDGVISKPRTLLPGKPFL
                     VLNALTVNKNQQLTIQPGVKIHFHKDAFCWVEGAINAVGTVENPIVFEGDRLEKMYSD
                     VPSQWEGIKISPGRMSS"
     gene            complement(1091..>1961)
                     /locus_tag="PKJ43_06785"
     CDS             complement(1091..>1961)
                     /locus_tag="PKJ43_06785"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNZ72309.1"
                     /translation="PARFLLLGSASPELIRDSSESLAGRIAYHQLFPIGLTEIPDTVS
                     QNDLWLKGGFPNMLFTDKPNLARRWMENFISTYLNRDLLQLGLNASPKVIRNLWTMMA
                     HLNGQLLNATTIGNSLGITTPTAKRYVDFLEDAFLLKSLHPFHWNISKRLVKTPKVFL
                     TDTGILHHLLGVNDFVSLSGNPIIGSSWESFVFNQVLAVKPDEIDLYFYRTHHGAEVD
                     LVFARGYTVVATAEVKYSNSPQLSKGNFQAFEDLKSPVNYVITPSSDDFLIKENIRVC
                     SFPDFLKSYFQNL"
BASE COUNT          627 a          444 c          383 g          507 t
ORIGIN      
        1 tgctgctcat tcttcccgga ctaattttaa ttccttccca ttgcgaagga acatcactat
       61 acattttttc taagcggtca ccctcgaaaa caattggatt ttcaacagtt ccaacggcat
      121 ttatagcccc ctcgacccaa caaaaagcat ctttatgaaa atggatctta actccaggct
      181 gtatggttaa ttgctgattt ttgttcacag tcaacgcatt gagtaccaga aaaggcttgc
      241 ccggcaaaag agttcttggt ttcgagataa caccatcgag caaaatcaca tcctgaccgt
      301 atgccactaa atcaacatcc tgagataccc cgccaacagt aaacactaca gaatctttca
      361 caaccatagg ctgatcaaca ccagtcgggt caatggtcac ttcaacaaaa atatatatgc
      421 tatcctgtcc ccgaaggctg atgttttgag ctttattaac catttcccca tcaatattca
      481 atcggtaaaa cgattggtca ccaccggcca agactatcga agaaatttca accggattaa
      541 ccgaagggtt aattacctta aacgcctgag ttgttgaacc aaacgtggtg aaaatggtat
      601 cgaacataac ggtgtcggta gaaaaagcca gtttcgcatc gccagtaacc agatattttt
      661 ccttctcgca agaaacaact atcatccaag atagtaaaat aagcaaaatc gaatatttta
      721 gacttttcac agaagtgatc atttatatag acaaacaaat aaacgcaaga atcgggaaaa
      781 aattacacaa cgtgttactt tcctggctca atacctaaat agatatcctt gcaagttctt
      841 ctaaaaacgc aacaaaaata taaaacaaat cgactaatac cgattaaaaa atgtaacagt
      901 ccaatcttcg ctcactcgat ttgccttgac atttctattc atcggcatta tgcctgagtg
      961 gtttggtctg tttttaaatt gcacttggta taatataaga acaacccgac tcctattatt
     1021 gcaccaacgt tttagcaagt atgattatcc ccattataca cccacgctaa gtttggtcaa
     1081 aaggaaattg ttacaaattt tgaaagtagc ttttcaaaaa gtctggaaac gagcataccc
     1141 gaatgttttc ctttatcaga aaatcgtcgg acgaaggcgt aatcacataa ttaacaggcg
     1201 attttaaatc ttcaaatgcc tgaaaattac ccttcgagag ttgtggcgag ttggaatatt
     1261 taacttcggc cgtagcaaca acggtgtagc ctcgggcaaa aaccaaatcg acttcggctc
     1321 cgtgatgggt gcggtaaaaa taaagatcaa tctcgtcggg tttcactgcc agcacctgat
     1381 taaatacgaa cgattcccac gaactgccaa ttatggggtt gcccgaaagc gaaacaaagt
     1441 cgttcacccc caacaaatgg tgcaaaatac cggtatcggt aaggaaaacc ttaggagttt
     1501 tcaccaaacg cttcgagatg ttccaatgaa acgggtgcag gcttttcaac aaaaaagcat
     1561 cttccagaaa atcgacataa cgcttggccg ttggggttgt aattcccagc gagttgccaa
     1621 tggtggtggc gtttagcaat tgtccgttta ggtgcgccat catggtccac aagttgcgga
     1681 ttaccttggg cgaagcattc agtcccaatt gcagcaaatc gcggttcagg taggtactga
     1741 tgaaattttc catccaacgg cgcgcaagat tcggtttatc ggtaaaaagc atattgggaa
     1801 aaccaccttt cagccacaaa tcgttttgcg aaaccgtatc cggaatttcg gtaaggccaa
     1861 tggggaaaag ctgatgatag gcaatgcgcc cggccaacga ttccgacgaa tcgcggatta
     1921 actcgggaga tgcagaacct aaaagcaaaa aacgggcggg c
//
LOCUS       DAOPUW010000378         1958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_63275_length_1958_cov_2.944298, whole genome shotgun sequence.
ACCESSION   DAOPUW010000378 DAOPUW010000000
VERSION     DAOPUW010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63275_length_1958_cov_2.944298"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1958)
                     /gene="mutS"
                     /locus_tag="PKJ43_06790"
     CDS             complement(<1..>1958)
                     /gene="mutS"
                     /locus_tag="PKJ43_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNZ72310.1"
                     /translation="ASVHFDRKMAGAAFLDISTGEFLTAEGPFEYIDKLLNSFQPKEV
                     LFQRGKNDDFAALFGHKFYTFNLEDWVYASEAATDRLHRHFEVKSLKGFGVQKLEYGI
                     IAAGAILYYLDITQHHHIKHISNLSRIEEERFVWLDRFTIRNLELINPLNEGGKTLLS
                     VIDKTLTPMGARMLKRWISFPLKNVKAINSRLDMVECLVQKTELKEQIEENIRVIGDL
                     ERLISKVAVQRINPREVVQLKIALKAIEPIRRLCIDSGNESLAGLGEQLNSCKIIADR
                     IEKELYPDPPTQIIRGNTIAEGVSAELDDLRQIKSHGKDYLLKMQEREITQTGITSLK
                     ISFNNVFGYYIEVRNTHKDKVPQEWIRKQTLVGAERYITEELKEYEEKILGAEEKILA
                     LETRIYNDLVMALTEYIQAIQLNAGILSQIDCIVSFATVAEQNEYVKPTVSEENFLRI
                     KEGRHPVIEQELPIGESYISNSVYLDREEQQIIIITGPNMSGKSALLRQTALIVLMAQ
                     IGCFVPAKAAEIGYVDKIFTRVGASDNISLGESTFMVEMNEAASILNNLSDRSLILLD
                     ELGRGTSTYDGISIAWSIVEYIHDHPKFKAKTLFATHYHELNEMEHLFDRIKNYNVTV
                     KEIDNKVIFLRKLVRGGSNHSFGIHVAR"
BASE COUNT          478 a          429 c          435 g          616 t
ORIGIN      
        1 attcgtgcca cgtggatacc aaaactatgg ttgcttccgc ctctcaccag cttgcgtaag
       61 aaaatcacct tgttatcgat ttcctttacc gtaacattat aattttttat tctgtcgaac
      121 aggtgttcca tttcgttcag ctcgtggtaa tgagtggcga acagggtttt tgctttaaat
      181 tttgggtgat cgtgaatata ttcaacaata gaccaggcaa ttgaaattcc atcgtaagtg
      241 cttgtacccc ggccaagttc gtcgagcagt attaagcttc ggtcagaaag gttattcaaa
      301 atgctggctg cttcgttcat ttcaaccatg aatgtagatt ctcccagcga aatgttatct
      361 gaagctccga cccgggtaaa aatcttgtcg acatacccta tttcagcagc ttttgccgga
      421 acaaaacacc cgatttgagc catcagcacg atgagcgcag tctgccttag caaagccgat
      481 ttacccgaca tgttgggtcc ggtaatgata atgatttgct gttcttcgcg gtcgaggtac
      541 acgctgttcg atatgtagct ctcgccaata ggcagttctt gttcaatcac cgggtggcgg
      601 ccttctttaa ttcgcaggaa attttcttca gagaccgtcg gttttacata ttcattttgt
      661 tcagcaacag tagcaaaaga tacaatacag tcgatttgcg aaaggattcc ggcgttaagc
      721 tgaatggctt gaatatactc ggttagcgcc attaccaaat cgttgtaaat tcgggtttcg
      781 agcgccagta ttttttcttc ggcccccagt attttttctt cgtattcttt aagttcttcg
      841 gttatgtatc gctcggcgcc caccagggtc tgttttctga tccattcctg aggaacttta
      901 tctttgtgtg tgttacgcac ttcaatgtaa tatccaaaaa cattgttgaa gctgattttt
      961 agcgaagtaa tgccggtttg ggttatttcg cgttcctgca ttttcagcag atagtctttt
     1021 ccgtgagatt taatctggcg caaatcgtcg agttcggcac ttaccccttc tgcaatggtg
     1081 tttcctctaa tgatttgggt tggaggatcg ggataaagtt ctttctcaat ccgatcggca
     1141 attattttgc aggaattaag ctgttctccc aaacctgcaa gactttcgtt acccgagtcg
     1201 atgcatagcc tgcggatggg ttcgatggct ttcagggcaa ttttaagttg aacaacctca
     1261 cggggattaa tacgttgaac ggcaactttc gatattagtc gctcaaggtc gccaattacc
     1321 cgaatatttt cttcaatttg ttctttcagc tctgtttttt gaacaaggca ttccaccata
     1381 tcgaggcggc tattgattgc ttttacgttt ttcagcggaa aagaaatcca tcgcttcaac
     1441 attctggccc ccatgggtgt aagggtttta tcgatgacgc tgagcagggt tttcccacct
     1501 tcgttcaatg ggttgatcag ctcaaggttt cgtatggtaa acctgtcgag ccaaacaaag
     1561 cgttcctctt ctatcctcga gaggttcgaa atgtgtttta tatgatggtg ctgggtaata
     1621 tcgagataat ataaaattgc gccggccgca ataattccgt actcaagctt ctgtacacca
     1681 aatcctttta gtgacttaac ttcgaagtgg cggtgtaacc ggtcggtagc tgcttcggag
     1741 gcatacaccc aatcttcgag attgaacgtg taaaatttat gaccaaacag ggctgcgaaa
     1801 tcgtcgtttt ttccgcgttg aaaaagcact tctttgggtt gaaacgagtt cagcagctta
     1861 tcaatatatt cgaaaggtcc ttcagcagtt agaaattcgc cggtcgaaat atcgagaaag
     1921 gcagctccgg ccattttgcg gtcgaaatga acggaagc
//
LOCUS       DAOPUW010000379         1957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_63309_length_1957_cov_3.952681, whole genome shotgun sequence.
ACCESSION   DAOPUW010000379 DAOPUW010000000
VERSION     DAOPUW010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63309_length_1957_cov_3.952681"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            400..1134
                     /locus_tag="PKJ43_06795"
     CDS             400..1134
                     /locus_tag="PKJ43_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNZ72311.1"
                     /translation="MQKIVPNISVDCVIFGFDFKQLNVLLVERKLQDEKSGETLIDDW
                     TLTGYHIFEDEDFDTAAARILKGLSGLDNIYLEQFYAFGKVSRVDSPKDNIWLKHQHE
                     GFSSRIVTVGYYSLIDNSTVTLQLQERNVKWFSITDLPELGFDHQEILNKALEKLRSK
                     LRSEPIGFELLPEKFTLSQLQKLYEVILGTTYDRRNFRKKVLQMKYIIPLNEKQKAVK
                     HKPAQLFIFSRDVYERTKRERFDFSI"
     gene            1207..>1957
                     /locus_tag="PKJ43_06800"
     CDS             1207..>1957
                     /locus_tag="PKJ43_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ72312.1"
                     /translation="MNVVNSKLKFTEKVGYALGDGAANIAWRGVATFLLIFYTDVFGI
                     TAAAAGLLMLIARSFDGLVDIGMGIVCDRTNSKYGKFRPWILWTAIPLAVTLSLLFTT
                     PNLGPTGKIVYAYVTYLLFFIIYTANNIPYGALMAVMTGDDKERTSLGSYRMVGAFTG
                     GMIVQGALLFLVTYFGNIDPTIDVQKLDKEKFLITVSAPQNVHSVNIESKHGVAQFQW
                     NDSTLATEANKPAPRISFAMEASKPYSFIAQG"
BASE COUNT          626 a          378 c          366 g          587 t
ORIGIN      
        1 aaatggctca acaagaattt aaggacatta aatttcactt cacttctgaa ttttagtaca
       61 attagtcctt cgttgcacat ccaaagcctc gtataatcta tgatggctta aattgtcata
      121 taaccgtttc attcgacttc agtaaaggct aaaaaacaag tgtatatcat acgcatttat
      181 aatatcctcc ttattctaca aaaaagttca aaattgaagt tcaggacaaa tagcgaatgg
      241 tataaaaagt ggaattaata tgacatgttt taacttgttt tttcgatagt tataaaaata
      301 tgcaatgagc tttatgtaaa aatttcatca tcgatgttgg ggttcaaaaa aatttaatat
      361 ttttacaatg tgtaaatttc acacactaac aaagactcaa tgcagaaaat tgttccaaat
      421 atttcggttg actgtgtaat tttcgggttc gactttaagc agttgaatgt tcttttagtt
      481 gaaaggaaac ttcaggatga aaaaagcgga gaaaccctaa tcgatgactg gacacttacc
      541 gggtaccata tatttgaaga tgaagatttt gacactgcag ccgcccgcat tcttaaaggg
      601 ctatcggggc tcgacaacat ttaccttgaa caattttatg cttttggaaa agtatcccgg
      661 gtagatagcc ccaaagacaa catctggttg aaacaccaac atgaagggtt cagcagcaga
      721 attgttaccg ttggttatta ttcacttatc gacaattcaa cagttaccct gcaacttcag
      781 gagcgtaatg taaaatggtt ttccattacc gaccttcccg aattgggttt cgaccatcag
      841 gaaatcctta ataaagcctt ggaaaaactt cgcagcaaat tacgctctga acctattgga
      901 tttgaattat tgccagaaaa gttcactctg agtcagttgc aaaaattata tgaagtcata
      961 ctcggaacaa cctacgaccg acgaaacttc agaaaaaaag tgcttcagat gaaatacatc
     1021 atccctctta acgaaaagca aaaagcagta aaacataaac cggcacaatt attcattttc
     1081 agccgcgatg tatatgaacg caccaaacga gaacgtttcg atttttcgat ttaacccagt
     1141 tttatataac acccagtcaa ttaattaact aactacaaag tatttaattt ttaaaaaccg
     1201 aaacagatga atgtagtcaa cagcaaattg aagtttacag aaaaagtagg ttacgcgctt
     1261 ggcgatggag ctgccaatat tgcatggaga ggtgttgcaa ctttcttact aatattttac
     1321 accgatgttt ttgggattac cgctgctgcc gctgggctgc taatgctgat tgccagatca
     1381 ttcgatggat tggtagacat tggtatgggt attgtttgtg accgcaccaa ttcaaaatat
     1441 ggcaaatttc gtccctggat tttatggact gccattccat tggctgtaac tttatcactg
     1501 ctctttacta ctccaaacct tggtccaaca ggtaaaattg tttacgccta tgtgacttat
     1561 cttctgtttt ttatcattta tactgcaaac aacattcctt atggtgcgtt gatggctgtg
     1621 atgacaggcg atgacaaaga gcgtacaagc ctgggctctt accgcatggt aggcgctttt
     1681 accgggggta tgattgttca gggagcttta ttgttcttag ttacttattt tggaaatatt
     1741 gacccgacaa ttgatgtaca gaaacttgac aaggaaaagt ttttgataac agtttcagca
     1801 ccacaaaacg tacacagcgt aaatattgaa tcgaaacacg gagtagcaca gtttcagtgg
     1861 aacgattcta cattggccac tgaagccaac aaaccggccc cccgcatcag ctttgccatg
     1921 gaagccagta aaccctattc atttattgct cagggtg
//
LOCUS       DAOPUW010000380         1956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_63357_length_1956_cov_4.827459, whole genome shotgun sequence.
ACCESSION   DAOPUW010000380 DAOPUW010000000
VERSION     DAOPUW010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63357_length_1956_cov_4.827459"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PKJ43_06805"
     CDS             <1..451
                     /locus_tag="PKJ43_06805"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNZ72313.1"
                     /translation="DKQFGLTQSLILIRFADVLLMHSELTETATGLNRVRQRANLPEV
                     GYSLSALKKERRYELCFEALRWNDLRRWGDVADIVANQTGVSLLNRGNEDKYTFGQYD
                     FLNRYNATKGFWKIPDSQITLSEGVLEQNEGWDEASSDWTKLPYPTL"
     gene            547..1707
                     /locus_tag="PKJ43_06810"
     CDS             547..1707
                     /locus_tag="PKJ43_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HNZ72314.1"
                     /translation="MKRSILFIVLVGWVFQVFAQENYKGVDPNHEVRFYRAKEFYDVP
                     LIKQVDVDRLPKNVIFMIGDGMGIAQVSAALFANKGKLFLNNFVHCGYSQTQSASDFI
                     TDSSAGGTALSTGTRTRNGYVAKDTAHKDLTTILEYAESKKLKTGLISTSAVTHATPA
                     SFIAHQDARESYEDIALDFLKTDIDLFIGGGYNHFARRSDGRNLLDSLKNRGYFVSTT
                     IDSILEKGSTKLACLTAPEHTGRLPERGNMLPEATRMALEILSRDSAGFFLMVEGSQI
                     DWGGHNNDTRYVVQETLDFDKAIGEALKFAANDGQTLIVVTADHETGGMSLLGGDIEK
                     GFIHGGYSTGNHTGVMVPVFAYGPGAFLFTGIMKNTDIFNSVYKLLIAETRE"
BASE COUNT          558 a          383 c          446 g          569 t
ORIGIN      
        1 cgataagcag tttgggttaa cccaaagcct gatactaatc agatttgccg atgtgctgct
       61 tatgcactct gaacttaccg aaacagctac cgggttgaac agggttcgcc agcgtgccaa
      121 tttgcctgaa gttggctatt cgcttagtgc gctaaagaaa gaacgccgct acgaactttg
      181 tttcgaagca ttacgctgga acgaccttcg ccgttggggc gatgttgctg atattgttgc
      241 taatcagact ggcgtgtcgc tgttgaacag agggaatgaa gataagtata cctttgggca
      301 atacgatttt cttaaccgct acaatgcaac aaaaggcttc tggaaaattc ctgactcgca
      361 aattacttta tctgagggtg ttcttgagca aaatgaaggt tgggacgaag catcttccga
      421 ttggacaaaa ttaccatatc caacccttta acaggggcaa ttatctacaa tagtttttta
      481 ggttaaggcg tttcatgctt cttgccacct ggtttagggg catgaaacgc attttaaatt
      541 tttccgatga aaagaagtat actgtttatt gttttggttg gttgggtatt tcaagtattt
      601 gcccaagaga attataaagg agttgatcca aaccatgaag tacggtttta ccgggcaaaa
      661 gaattttacg atgttccact cataaaacaa gttgatgttg accggcttcc aaagaatgtc
      721 atttttatga ttggcgatgg catgggtatt gcacaggtct ctgctgcgct ttttgctaat
      781 aaagggaaat tattccttaa taactttgtg cattgtgggt attcgcaaac gcaatcggca
      841 agcgatttca ttaccgattc ttctgcgggg ggaacagctt tatcaacagg gacccgcacc
      901 cggaacgggt atgtggcaaa agacactgcg cacaaagatc ttactactat tcttgaatat
      961 gccgaaagta aaaagttgaa aaccgggctt atatctacat cggctgtaac tcatgccaca
     1021 ccggcgtcat ttattgccca tcaggatgct cgcgaatcat acgaagatat tgccctcgat
     1081 tttctgaaaa ccgatattga tctgtttatt ggtggggggt acaatcattt tgcccgtcgt
     1141 tccgatggac gaaatttgct cgatagtttg aaaaaccggg gatattttgt ttcaacaacg
     1201 atcgacagta ttcttgaaaa aggttcaact aaacttgcat gccttactgc ccccgaacac
     1261 accgggcggt tgcctgagcg cgggaatatg cttcccgagg ctactcggat ggcgttagaa
     1321 attctttcgc gcgattcggc aggctttttt ctcatggtcg aaggttctca aattgattgg
     1381 gggggacata ataacgatac ccgctatgta gttcaagaaa cattggattt cgacaaggcg
     1441 ataggcgagg cacttaaatt tgctgccaac gatggtcaga cattgattgt tgttacagca
     1501 gatcatgaaa ccggaggcat gtcattgttg gggggagaca ttgaaaaggg atttattcac
     1561 ggaggctaca gtacaggaaa ccacactggg gtgatggttc cggtatttgc ttatggcccc
     1621 ggagcattcc tgtttaccgg gataatgaaa aacaccgata tttttaactc agtttacaaa
     1681 ttgctcattg ctgaaaccag agaataaatt tccaacaata cagaagataa acgattttat
     1741 ataatagatt aattttcatt agattattag agagctgaat tttcggggga ttgccacatc
     1801 ggcaatccct tttttgataa aacaattttg taaccgacat aaaaaacgga tcacttcata
     1861 tttggggttg acactttata atctttattt agcctcaact tgaaggaaga aaaaattcaa
     1921 cagctttttt gacagaatgg ccactaatgc acgaat
//
LOCUS       DAOPUW010000381         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_63732_length_1949_cov_2.260296, whole genome shotgun sequence.
ACCESSION   DAOPUW010000381 DAOPUW010000000
VERSION     DAOPUW010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63732_length_1949_cov_2.260296"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /gene="pyrH"
                     /locus_tag="PKJ43_06815"
     CDS             <1..255
                     /gene="pyrH"
                     /locus_tag="PKJ43_06815"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664807.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNZ72315.1"
                     /translation="ADALLKGTRVDGIYTADPEKDPKAQKYDTLTYDEAYSKKLKIMD
                     LTAFTMCSENGLPIIVFDMNTPGNLKRIVMGEKIGTLVKK"
     gene            287..850
                     /gene="frr"
                     /locus_tag="PKJ43_06820"
     CDS             287..850
                     /gene="frr"
                     /locus_tag="PKJ43_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNZ72316.1"
                     /translation="MKEEADFIVETTTERMNKAIEHLENELRGIRAGKASPSMLMSVM
                     VDYYGTLTPLQQVSSIGAPDARTITIQPWEKTMIPTIEKAIMAANLGFNPQNNGEIIR
                     VVVPALTEERRKQLVKQVKNEGENARVSLRNARRDGNEEIKKLQKGGLAEDLAKDYEA
                     EIQKLTDSFSKKVDELLARKEHDILTV"
     gene            941..>1949
                     /locus_tag="PKJ43_06825"
     CDS             941..>1949
                     /locus_tag="PKJ43_06825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ72317.1"
                     /translation="MQKVLIVTYYWPPSGGSGVQRWLKFIKYLRKFGIEPVVYTPKNP
                     DISLFDTSLHKDIPDNLTVLQRRIFEPYALFRLFARRKTNFGVGFTSIEGHTPNRFTR
                     LSIWIRGNCFIPDPRVLWIKPSIRFLKKYICKNGIEAIVTTGPPHSMHLIGMALKKKL
                     NIAWVADFRDPWTNIDFIDNLKLNSRSLAKHLHLEKQVVESADCVVVVSPQMKKDFEG
                     YNPKQVEVITNGFDADDFPKNVQKRDEQFTITHVGTIPPNRNCPVLWNVLSEMAKADE
                     EFSAKLSIQLVGNVDSSVINSIESEGLINHCKFLGYVTHEEGIRFMQRSQVLLLLVNN
                     SP"
BASE COUNT          618 a          403 c          433 g          495 t
ORIGIN      
        1 gctgatgctt tgctgaaagg aacacgagtt gatggtattt acaccgctga cccggagaaa
       61 gacccaaagg cccaaaaata tgacactctc acttatgacg aggcatacag caaaaagttg
      121 aaaataatgg atcttactgc atttaccatg tgcagtgaaa acgggctacc cattattgtt
      181 tttgacatga atacccccgg caacctaaaa cgtattgtaa tgggtgagaa aattggtact
      241 ttggttaaaa aatagataat tttatacgaa aaaatgtttt cacgccatga aagaagaagc
      301 tgattttatt gttgaaacca ccaccgagcg catgaacaaa gccattgagc atttggaaaa
      361 tgagctgcgc ggaatcaggg ccggaaaagc aagtccgtct atgttgatga gtgtaatggt
      421 tgactactat gggacactca ctccacttca acaagtctct agcattggtg caccggatgc
      481 cagaacaata accatacaac cctgggaaaa aaccatgatt cccaccattg aaaaggcaat
      541 catggctgcc aacctgggat tcaatccaca aaacaacggg gaaatcatca gggttgtcgt
      601 tccggcactc accgaagaac gtcgcaagca gttggtgaag caggttaaga acgaagggga
      661 aaacgccaga gtgagcctta ggaatgcccg tcgcgatggc aatgaagaga ttaaaaaact
      721 tcaaaagggt gggcttgctg aagacttagc caaggactat gaggctgaaa tacaaaagtt
      781 gaccgacagt tttagcaaaa aagtggatga acttcttgct cgaaaagagc atgatattct
      841 tacagtatag ccctgaagtt tcattttaat gccttttctg actaataagg gctgtttttc
      901 aggtattaac taagcctata aaaaggggtt tgaacggtaa gtgcaaaagg tactgattgt
      961 tacatattac tggccgccat ccggcggaag tggggtacag cgttggctga aatttatcaa
     1021 atacctgaga aaatttggca tagagccggt agtttataca ccaaaaaacc ctgatatatc
     1081 cctattcgac acttcgctcc ataaggacat cccagataat ttaacggtat tacaacggcg
     1141 gatattcgaa ccgtatgccc tatttagact tttcgcaagg cggaaaacca acttcggcgt
     1201 tggttttacc tccattgagg gccatactcc caaccgattt acaaggctgt ccatttggat
     1261 aagaggaaat tgctttattc ccgatcccag agtcctttgg atcaagccct ccattcgatt
     1321 tctgaaaaag tatatctgca aaaatggtat cgaagccatt gtaactacag gccccccaca
     1381 tagcatgcat ctcattggta tggcattaaa gaaaaaacta aacattgcat gggtggctga
     1441 ttttagggat ccgtggacaa atattgattt catagacaat cttaaactga acagtcgctc
     1501 cctggccaaa catctgcatt tagaaaaaca ggtggtagaa tctgctgatt gcgtggtggt
     1561 ggtgtcgcct caaatgaaaa aagactttga gggttataat cctaaacagg ttgaggtgat
     1621 taccaacgga tttgatgcgg acgacttccc caagaacgtt cagaagcgcg atgagcaatt
     1681 taccataacg catgtaggta ccataccccc taataggaat tgccctgtcc tttggaatgt
     1741 tttaagcgaa atggctaaag ccgatgaaga attctctgca aaactatcaa ttcaactggt
     1801 gggcaatgtc gattcctcag ttattaatag cattgagtcc gaagggctca taaaccattg
     1861 taagtttttg ggttacgtta cccacgaaga ggggataaga tttatgcaac gctcgcaagt
     1921 ccttttgctt ttagtaaaca acagcccca
//
LOCUS       DAOPUW010000382         1944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_63960_length_1944_cov_3.155109, whole genome shotgun sequence.
ACCESSION   DAOPUW010000382 DAOPUW010000000
VERSION     DAOPUW010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63960_length_1944_cov_3.155109"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(270..644)
                     /locus_tag="PKJ43_06830"
     CDS             complement(270..644)
                     /locus_tag="PKJ43_06830"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNZ72318.1"
                     /translation="MKEITKAQEEILKALWQVNNGAVSDVLDVLPDPKPAYNTVATVI
                     KVLEAKGYVAHKTYGKTNVYYPAISKTEYGQFVMKETFKGLFNGSLNQMMSCFVKNKD
                     VSIQELEELKQLLESEIEKQKK"
     gene            complement(856..>1944)
                     /locus_tag="PKJ43_06835"
     CDS             complement(856..>1944)
                     /locus_tag="PKJ43_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNZ72319.1"
                     /translation="LGLKASWESVVNFKNIEATKRTEIISANAQWFEDNSPVDKRFKK
                     ETVKGVTAKVITAAMLGGDCYPATPIGINLPNADWIRKEHGSKSVTIENITYAYSKAA
                     EGNGFMEEFAYSEKEIENEKTYGFQADNLHTDMHECLGHGSGQLLPGVSGDELKAYGS
                     VIEEARADLFALYYMGDKKMVELGLLPDAEAYKAAYDDYIRNGLMTQLTRIKLGDNIE
                     QSHMRNRQLISKWVFEKGTANNVIEKKMKEGKTYFVINDYNRVRTLFGELLSEIQRVK
                     SEGDFEAAKNLVENYAVKIDQELHAEVIERYSKLNLAPYSGFVNPVYNVETDANGKIS
                     NITLDYTEGYAEQMMRLAKDHSWLPTYN"
BASE COUNT          536 a          399 c          335 g          674 t
ORIGIN      
        1 taagttcgtc aattttgata cttgatttgt ttccgggatc attcgttgtt tctgttatgg
       61 acgaagtctc aaccccagtt ctatatttcg aaaaatcaat tgggatttgg aaaaagttca
      121 taggtaaaat caatgataaa acaatggatg tcagcaaata gaagcgaatt agtttaaaat
      181 tcgtctcttt tttgaatgcc agcaaataaa caaaataaaa gacactcaaa cagattgctg
      241 aaatgagcag ataacctgat actcccattt tatttctttt gtttttctat ttcagattcc
      301 aataattgct tcaactcttc aagctcctga atgcttacat ctttgttttt cacaaaacac
      361 gacatcattt ggtttaatga accattaaac aaacctttaa aggtttcttt cattacaaat
      421 tggccatatt cggttttaga aatggcagga tagtacacat ttgtttttcc atacgttttg
      481 tgagcaacat acccctttgc ttccaacact ttaataaccg ttgcaaccgt attataagca
      541 ggtttaggat caggtaatac atccagtaca tcacttacag cgccattatt aacctgccaa
      601 agtgctttta atatctcttc ctgtgctttt gtaatctctt tcatatatta ctcttttctg
      661 caaatatatt tcaaataaac gacattacta ctatgattat agtatttatt ttaatgtttt
      721 ttgaatgcag ttcttaactt catgaatatc tgtaaataaa aatagaagag gttgacccat
      781 ctcggagcca acctcttgta attattaaat tccagtaaat tttaaatacg aatggttttt
      841 tctatccgct ctttactaat tatacgttgg caaccacgaa tgatcttttg ccaaacgcat
      901 catctgctcg gcataacctt cggtatagtc gagggtgatg ttggaaattt taccgttagc
      961 atcggtttca acgttgtaaa cagggttcac aaagccagag tatggggcaa ggttcaattt
     1021 ggaatacctt tcaataactt cggcatgtaa ttcctgatca atttttactg cataattttc
     1081 aacaaggttc tttgctgctt caaagtcacc ttctgacttt actcgttgta tctcagacaa
     1141 aagttcacca aagagggtcc ttactctatt ataatcgtta attacaaagt aggttttccc
     1201 ttctttcatc tttttctcga ttacattatt ggcagtccct ttttcaaaaa cccatttcga
     1261 gattaactga cggttgcgca tgtgcgattg ttcaatgttg tcgccaagtt taatgcgggt
     1321 taactgggtc attaagccgt ttctgatgta gtcatcgtaa gctgctttat acgcttcagc
     1381 atcgggcagc aaacctaatt caaccatctt tttgtcgccc atgtaataaa gggcaaacag
     1441 gtcggcacgc gcttcctcaa taaccgatcc gtatgctttt agctcgtcgc ccgaaacacc
     1501 aggcagcaat tgccctgagc catggcccag gcattcgtgc atgtcggtat gtaagttgtc
     1561 ggcctggaaa ccatatgttt tctcattctc aatttctttt tcagaatagg caaattcttc
     1621 cataaaaccg tttccttcgg cagcttttga atatgcatag gtgatgttct cgatagttac
     1681 cgattttgag ccatgttctt tacgaatcca gtcggcattg ggaaggttga tgccaattgg
     1741 ggtagcagga tagcaatcgc cgcctagcat agctgctgta attactttgg cagtaactcc
     1801 ttttaccgtt tcttttttga agcgtttatc tacaggtgaa ttatcttcga accattgggc
     1861 attggcacta attatttcgg tgcgttttgt cgcttcaata tttttgaaat taaccacgct
     1921 ttcccaactt gctttcaagc ccag
//
LOCUS       DAOPUW010000383         1943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_64004_length_1943_cov_3.982521, whole genome shotgun sequence.
ACCESSION   DAOPUW010000383 DAOPUW010000000
VERSION     DAOPUW010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64004_length_1943_cov_3.982521"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="PKJ43_06840"
     CDS             <1..1268
                     /locus_tag="PKJ43_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNZ72320.1"
                     /translation="IPIKPIKRFRFTQPEEEGFDSKILTEKIDSMTNNAIKNGYFPGC
                     QVLIAKNGAVIFQKAYGYLTYDTTEKVSNQTLYDWASVTKITGPLPLLMKLYQDSIIK
                     LDVPFSTYFESFSKSNKAKITLREILTHQAGFKSGISFQTKLIREEQKKPHSVFSQSP
                     SSQFPVRISSSLYTHADCKQQMFEEIKNSELQQSKKYVYSDIGFYLFPEMISKITGKD
                     YEQYLYNTIFKPLGANKVCYNPYKVYPLPQIAPTERDDYFRKETLRGFVHDEGAAILG
                     GVSGNAGLFGRSVDLAILMQCYLQQGQYGDVFIADSNTVEEFTRVQFPENNNRRALGF
                     DKPYMNNKSLSGDDAYPAPDVSDKSFGHSGFTGTFTWVDPETNLLFVFLTNRVFPTRE
                     NTKLIKNNFRPRMLQTIIECEKSFRHCHH"
     gene            1493..1747
                     /locus_tag="PKJ43_06845"
     CDS             1493..1747
                     /locus_tag="PKJ43_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005827755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNZ72321.1"
                     /translation="MTDRITFNELRKIKDSLPDGSMLQLANEFGLEVETVRNYFGGAN
                     YTKGKSVGMHIEPGPNGGIVELDDSAILDRAKEIIAEHSH"
BASE COUNT          675 a          396 c          367 g          505 t
ORIGIN      
        1 gtatacccat aaaaccaata aaacgattca ggtttactca acccgaagag gaaggattcg
       61 actcaaaaat actgactgaa aaaattgatt cgatgacgaa caatgcaatt aaaaatggct
      121 acttccctgg ctgccaggta ctcatagcaa aaaacggcgc tgttattttt caaaaagcat
      181 atggatacct cacctacgat accactgaaa aagtaagtaa ccaaaccctc tacgattggg
      241 catcggtgac caaaataaca ggtccgctgc cgctcttaat gaaattgtac caagactcta
      301 tcatcaagct cgacgttcca ttcagcacat attttgagag ttttagtaag tcgaacaaag
      361 caaaaataac cctccgggaa atattgactc atcaagccgg atttaaatca ggcatttcat
      421 ttcaaaccaa acttattcgt gaagaacaga aaaagccaca ttcagtgttc agccagtccc
      481 cttcatcgca attccccgtt cgcattagct catcacttta cactcacgca gattgtaaac
      541 agcaaatgtt cgaagaaatt aaaaactcgg agttgcagca gtctaaaaaa tacgtgtatt
      601 ccgatatagg cttttacctt ttccccgaaa tgatttcaaa aattacggga aaagactatg
      661 aacaatacct ctataacaca attttcaagc ccctaggtgc caacaaagta tgctacaatc
      721 cctataaggt ttacccgttg ccccagattg cgccaaccga acgcgacgat tatttcagaa
      781 aggaaacact gcgaggattt gtccatgatg aaggtgctgc catattaggc ggtgtatcgg
      841 gtaatgctgg gcttttcggc cgatctgtcg atttggcaat actgatgcaa tgttaccttc
      901 agcaagggca atacggagat gttttcattg cagacagcaa taccgttgaa gaatttaccc
      961 gtgtacaatt tcccgaaaac aataaccgcc gtgcattagg cttcgacaag ccatacatga
     1021 acaacaaaag cctttcggga gacgacgcat accccgcacc agatgtaagc gataaaagct
     1081 tcggacatag cgggtttaca ggaactttca catgggtaga ccccgaaaca aatctacttt
     1141 ttgtttttct taccaaccgg gtttttccaa cacgggaaaa cacaaaactg ataaaaaaca
     1201 atttccgtcc acgaatgctt caaacaataa ttgaatgtga aaaaagcttt aggcattgcc
     1261 atcattaaaa attgttaaaa accagcttgg gtgttttgtt tcgtattatg tcaattacaa
     1321 cgtaatgttt ggatgctaga gagttgacga aaaagagcag aataatcagt taaaaatgat
     1381 ttcgtaatcg gaatggattt aaaataaaac ctaaaaaaca cattgccaat ccaaataatc
     1441 tgttaatttt atggtaattt aaagataagt taattttcta atcgtatatg tcatgacaga
     1501 tagaattaca tttaacgaac tgcgaaagat taaagacagt ttacctgacg gttcaatgct
     1561 ccaattagcc aacgaatttg gattagaagt tgaaaccgtc agaaactatt tcggtggggc
     1621 aaactacacg aaagggaaat cagttggcat gcacatagaa ccggggccaa acgggggaat
     1681 tgttgaactt gacgactctg ccatccttga tagggcgaaa gaaattattg cagaacattc
     1741 acattgagaa aaggagaatt tcattacaaa ggctgcccga aaggcagctt tcttttttat
     1801 ataacgtcag gaacattaga ttagctctgc attgtaaaaa gaaatgaaaa aatttattgg
     1861 aaaaatttgc aagtaaaaaa cattgatata tatttgcaac gcttttgaac aaagacacac
     1921 cgggcggtta gctcagctgg ttc
//
LOCUS       DAOPUW010000384         1943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_64023_length_1943_cov_2.619174, whole genome shotgun sequence.
ACCESSION   DAOPUW010000384 DAOPUW010000000
VERSION     DAOPUW010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64023_length_1943_cov_2.619174"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..458)
                     /locus_tag="PKJ43_06850"
     CDS             complement(159..458)
                     /locus_tag="PKJ43_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72322.1"
                     /translation="MQRYTIGIILLFLCKFGYSQTDSIVPQLPRPAAAWESGYQQRIN
                     LMWGTRIPLYNPNPSNGNADSGKSEWSKLLAKMAWPVTNSSAKPLNHLSLPVAIW"
     gene            complement(458..>1943)
                     /locus_tag="PKJ43_06855"
     CDS             complement(458..>1943)
                     /locus_tag="PKJ43_06855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ72323.1"
                     /translation="FAAANGSFVGVGAGRIVMGCVIKNAVVGNHNIIQYAKEKYDSIA
                     QSTYPYKFGARIGVYGSNTFVANNYLPKPDSCFKYYQTTAITQQDKCNQAFGNHRKTL
                     IYDYGKAIGLDVNKCFLNPFGNTLPGYLAENVIIQDNNVYNHGNKGFEISGNWFLVRN
                     NKNVRDYLQEGADRYGLGPGWELTLDGFYESVPGGNGCLSDNLSRAFDMAGKNGWIDN
                     NFYNNTGSNPGNDGEGILWQAHGGMNSIWSFAITNNNGTSGYMAGYDVSHEGSLWAWN
                     TTTNIGNYKAGTMTDVAIVSNTGNVVTTGNDVLTTCPTNSPAAPEAPVVAFEAEKKYV
                     TISWTDASTNEIGFRIDRKDGTGEWKTIVFRPRKSAGSTFNEQIWRDYLYPKNINFQY
                     RVVAIDCNNNNAGASPASVILNSGSTANLIDFSNTEFKILKDKIVFNSIENHPYITIS
                     DISGKILVREEIKKNIFSFQNLASGIYLLIFQSDIKTITTKIVR"
BASE COUNT          544 a          426 c          384 g          589 t
ORIGIN      
        1 agttttcata agttgatgaa acatactttt aacatccgag taaatcaaat ctacctgagt
       61 tggatcattt gcggcaattt catctttcca acggaaataa tacatggcat atccggcgca
      121 actgaagggc gaataaaaac tccccacgtt gcttccgttc accatattgc gaccggtagc
      181 gataaatgat ttaagggttt tgcacttgag tttgtaactg gccaggccat tttagctaat
      241 aattttgacc actccgattt tccactgtca gcatttccat tcgaaggatt ggggttgtac
      301 aatggaattc gtgtccccca catgagattt atgcgttgct gataacccga ctcccatgct
      361 gcagctgggc gtggaagttg aggcacaata ctatcggttt gcgaataacc aaatttacaa
      421 agaaacagca gaattattcc tatagtatat cgttgcatta acgtacaatt tttgttgtta
      481 tagtttttat atccgattga aatataagca gataaatacc tgaagctaaa ttttgaaaag
      541 aaaaaatgtt cttttttatt tcctctctga caagtatttt tcccgaaata tctgaaatgg
      601 ttatatatgg gtgattttcg atggagttaa aaacaatttt atctttgaga attttaaatt
      661 cagtgtttga aaaatcaatg agatttgcgg ttgaacccga gttcaggatt accgatgcgg
      721 gtgaagcgcc cgcattgttg ttattacaat caattgccac aacacggtat tggaagttta
      781 tatttttcgg atacaagtaa tcgcgccaga tctgttcatt gaatgtacta ccggcacttt
      841 tacgtggtcg aaaaacaatc gttttccatt cgcctgtccc gtctttgcgg tcgatacgga
      901 aaccgatttc gttggtcgaa gcatcggtcc acgaaatggt tacgtacttt ttttcagctt
      961 cgaaagccac aacaggagct tccggagctg ctggtgaatt ggttgggcaa gttgtaagca
     1021 catcgttacc ggtagttacc acatttccgg tattgctgac aatggctaca tcagtcatag
     1081 tgccagcttt gtagttgccg atattggtgg tggtgttcca tgcccacaac gaaccttcgt
     1141 gcgagacatc gtaccccgcc atataacccg aagtaccgtt gttgtttgta atggcaaacg
     1201 accagatact gttcattccg ccatgcgctt gccagagtat tccttcacca tcgttaccgg
     1261 gattggagcc tgtattgttg taaaaattgt tgtcaatcca tccgtttttt cctgccatat
     1321 cgaaagcacg cgagagatta tcggacaagc aaccatttcc tccgggtaca ctttcgtaaa
     1381 agccatcaag cgtaagttcc caaccgggac ccaacccata gcggtcagcg ccttcctgca
     1441 gataatcgcg gacattttta ttattgcgaa ccaaaaacca atttcccgaa atttcgaacc
     1501 ccttgttgcc gtgattatac acattattat cctgaataat tacattttcg gccaaataac
     1561 ccggaagtgt attgccgaac ggattcagaa aacatttgtt gacgtccaaa ccgatggctt
     1621 ttccataatc ataaatcaac gtttttcgat gattgccaaa cgcctgattg catttatcct
     1681 gctgcgtaat ggctgtggtt tggtaatatt taaaacaact atcaggttta ggcagatagt
     1741 tattagcaac aaaagtattg cttccataca cgccaatacg cgcaccgaat ttataaggat
     1801 aagtggactg agcaatacta tcgtattttt ctttggcata ctgtataatg ttatgattgc
     1861 cgacaaccgc gtttttaatc acacaaccca tcacaatccg tcctgcacct acacccacaa
     1921 aacttccgtt ggcagccgca aaa
//
LOCUS       DAOPUW010000385         1941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_64124_length_1941_cov_2.728526, whole genome shotgun sequence.
ACCESSION   DAOPUW010000385 DAOPUW010000000
VERSION     DAOPUW010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64124_length_1941_cov_2.728526"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="PKJ43_06860"
     CDS             complement(<1..1174)
                     /locus_tag="PKJ43_06860"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNZ72324.1"
                     /translation="MRRLFMLSLSLFLFFSCSNQNENLRVVKNKAFVPFAPPVSYSEA
                     QKKADSIVMSMTIDERIDMIGGHNFFFVQGVPKANLPRLYLSDATQGVHLRKDLDSQL
                     EKSTAMPCPIALTSTWNRDLAHIYAKSIGEECRAGEIAVLLGPGMNIYRIAQNGRNFE
                     YFGEDPYLAARMIENYIVGLQSTGTIATLKHFMLNNTDFRRRTSNSIASERTMHEIYL
                     PAFKAGIDAGAMAVMTSYNQINGEWAAESKDVVSGILREKLGFKWLVMSDWWSVWHPE
                     KAMKSGLDLDMPGEGDLNWILFKKFGDSFIRSNAKRLLKEGKVSEDDINRMARNVIAT
                     ELAMELDKRPVKDVSYLANFNQHEEVALQAAREGIVLLKNEGNILPIKGTDIKILAT"
     gene            complement(1264..>1941)
                     /locus_tag="PKJ43_06865"
     CDS             complement(1264..>1941)
                     /locus_tag="PKJ43_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HNZ72325.1"
                     /translation="TYSLLKHFNLFNVEQIDGRERQHYQMFNEVRSKYYKEYRTCTDF
                     PAATGIGMLHGGVSIDSFSVAGNEHLNIIPISNPVQSESYKYGQTVLVGAPGCSLTQN
                     QPPQFERAKLISLGNQSRLIISGTASIKGQETVGLGDVEEQTRITIENIKALASPENL
                     KRQFPQLVAVPNRYSYIRVYVKHKKDFGKVRNICNEFYGQVPAMYVVADICRDNLLVE
                     IEAELIS"
BASE COUNT          569 a          432 c          370 g          570 t
ORIGIN      
        1 cagtggcaag tattttaata tcagttcctt ttatgggtaa aatatttcct tcatttttca
       61 gcaacacaat accttcgcga gcagcctgta aggccacttc ctcgtgctga ttgaaattag
      121 ccagataaga aacatctttc acgggtcgct tatcgagctc cattgccaat tcggttgcaa
      181 taacatttcg ggccatacgg ttaatatcgt cttccgatac ttttccttcc ttcaataagc
      241 gcttggcatt ggagcgaata aatgaatctc caaatttttt gaacagaatc cagttcaaat
      301 ccccttcgcc gggcatatca agatcgagtc ccgatttcat cgctttttca ggatgccaaa
      361 ccgaccacca atcggacata accaaccact tgaaaccaag tttttcgcga agaatacctg
      421 aaacaacatc tttgctttcg gcggcccatt caccattaat ttgattatat gaagtcatta
      481 cagccatggc tcctgcatcg atacctgctt taaaggctgg aagatatatt tcgtgcatgg
      541 tgcgttcaga agcgatagaa ttggatgtac gcctgcgaaa atcggtatta ttaagcataa
      601 aatgcttcag cgtggcgatc gttcctgtgc tttgaagccc tacaatataa ttttcaatca
      661 tccgggctgc caaatatggg tcttctccaa aatactcaaa gtttcttccg ttctgagcaa
      721 tacggtaaat gttcattcca gggccaagaa gaacggcaat ttctccagca cggcattctt
      781 cgccaatcga cttagcataa atatgagcaa ggtccctgtt ccatgttgaa gtcaaagcga
      841 taggacaagg cattgcagtc gatttttcga gttgagaatc aagatccttg cgcaagtgaa
      901 ccccctgagt tgcatcacta aggtaaagtc tgggcagatt ggctttggga accccctgta
      961 caaaaaagaa gttgtggcca ccaatcatgt caatacgttc atcgattgtc atcgacatca
     1021 caatagaatc ggctttcttt tgtgcttcgc tatacgatac cggaggtgca aacggtacaa
     1081 aggctttgtt ctttacaacc cttagatttt cgttctggtt agaacacgaa aagaacagaa
     1141 acaaacttag acttagcata aataatcttc tcatatttct ggtttttaat tgtatttatc
     1201 cgacaacttc atcactggaa attaatcacc agaaaaatag gaaaaagaaa aatagaaaaa
     1261 aagtcaggaa atcaattcgg cttcaatctc taccagtaaa ttatcgcgac aaatatcggc
     1321 tacaacatac atagcaggaa cttggccata aaattcgtta caaatattac gaacctttcc
     1381 aaaatctttt ttgtgtttta catataccct tatgtaacta taacggttcg gaacagccac
     1441 aagctgagga aactgccgtt ttaggttttc gggacttgcc aaggctttaa tattttcgat
     1501 ggtaatgcgt gtttgttctt caacatcgcc aagacctact gtttcctgcc cttttattga
     1561 ggccgtacct gaaataataa gccttgattg attacctagt gaaatcaatt tggccctttc
     1621 aaattgcggg ggttggtttt gcgtcaacga acaacctggt gcgccaacaa gtaccgtttg
     1681 tccatattta tatgattcgc tctgtacagg attgctgatc gggataatat tcaggtgctc
     1741 gtttccggca acagaaaaac tatcgatgga tacgccccca tgcaacatgc ctattcctgt
     1801 agcagcaggg aaatcggtac aggtacgata ttccttgtag tatttgctcc gcacctcatt
     1861 aaacatctga taatgttgac gctcacgacc atcaatctgt tcaacattaa acagattgaa
     1921 atgctttaac agggaatatg t
//
LOCUS       DAOPUW010000386         1939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_64233_length_1939_cov_2.054671, whole genome shotgun sequence.
ACCESSION   DAOPUW010000386 DAOPUW010000000
VERSION     DAOPUW010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64233_length_1939_cov_2.054671"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1911)
                     /locus_tag="PKJ43_06870"
     CDS             complement(<1..1911)
                     /locus_tag="PKJ43_06870"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNZ72326.1"
                     /translation="MRITFLTTSTILLMATIVSCTEARKSDENPFLSEYQTPFGVPPF
                     DKIGNSHYLPAFETGIEQQKKEIEQIVSNNEEPNFKNTIEALDYSGSLINKVGRVFFN
                     LTEANTNDELQAIAKEISPKLSLHLDEIYLNEKLFEKVKAVYQQKEKLNLNSEQAKLL
                     EETYKQFVRSGANLPKEKKEEMKKINEELSILTLQFGDNVLAETNNFKLVIDNKDDLV
                     GLPESVIQAAAETAGEPGKWVFTLQKPSWTPFLQYSQKRDLREKLYKAMYNRANNNNE
                     FDNKEIIKKILDLRTKKAQLLGFQNYAAYTLDDKMAKTPENVFELLNKLWDPALKNAK
                     AELAEMQAMATKEGDKITLQSWDWWYYAEKIRKEKYDLDENQLRPYFELNNVREGSFI
                     LANKLFGITFEEIKNIPLPHPDAKAFEVKDRDGSHIGVYYVDYFPRDSKRGGAWMDSF
                     RNQYRTTDGKKVSPVITNVCNFSRPTGNQPALLSFEEVTTLFHEFGHALHGLLSDCTY
                     ESLGGTEVPRDFVELPSQIMENWCSQPEMLRLFAKHYQTGEIIPQALIDKIVASGKFN
                     QGFETVEYLAASILDMDYHVLNTAEGLDVDKFETEQMQKIGLIEEIIPRYNFFNKANL
                     LHLFCFKLVDIQP"
BASE COUNT          510 a          389 c          383 g          657 t
ORIGIN      
        1 gggttggatg tcgacaagtt tgaaacagaa caaatgcaga agattggcct tattaaagaa
       61 attatagcga ggtataattt cttcaataag gccaatcttc tgcatttgtt ctgtttcaaa
      121 cttgtcgaca tccaaccctt ctgctgtatt cagcacatgg taatccatat cgagaataga
      181 agcagccagg tattcaacag tttcaaaacc ctgattgaat tttcctgaag ccacaatttt
      241 gtcaattaat gcctgcggta taatctcacc cgtctgatag tgctttgcaa ataagcgcag
      301 catttctggt tgactgcacc agttctccat aatttgcgat ggaagttcta caaaatcacg
      361 gggtacttcg gtgcccccca gactttcgta tgtgcagtcg gaaagcaatc cgtgcagtgc
      421 atggccaaat tcatggaaca aagtggttac ttcttcaaag ctcaacaatg ctggttggtt
      481 tccagtgggc ctggaaaagt tgcacacgtt ggtaattact ggcgacactt ttttaccgtc
      541 tgttgtacga tactgattac ggaaactatc catccaggca ccgccccgct tgctgtcgcg
      601 cggaaaataa tcaacataat aaacaccgat atgagaacca tcgcgatctt ttacttcgaa
      661 agctttagca tcgggatggg gtagtggtat gtttttgatt tcttcgaaag taataccaaa
      721 cagcttatta gccaaaatga aagagccttc acgaacatta tttaattcaa aatatggccg
      781 cagttggttc tcatcgagat cgtatttttc tttccttatt ttttcggcat aataccacca
      841 atcccaactt tgcaaagtga ttttatcacc ttctttggtg gccatagctt gcatttcggc
      901 caattctgct tttgcatttt tcaacgctgg gtcccaaagt ttattgagca attcaaatac
      961 attctcgggt gtttttgcca ttttatcgtc gagagtatag gctgcataat tttgaaatcc
     1021 aagaagttga gctttctttg tgcgcaggtc taatattttc tttatgatct ccttattgtc
     1081 gaattcgtta ttattatttg cgcggttata catggctttg tatagttttt cacgcaaatc
     1141 gcgtttttgc gagtactgta aaaatggggt ccagcttggt ttttgtaagg tgaaaaccca
     1201 tttccctggt tcgcctgctg tttctgcagc cgcctgaata acgctttcag gaagtcccac
     1261 aaggtcgtct ttgttatcga tcactaactt gaaattatta gtttcggcca gcacgttatc
     1321 tccaaattgt aaggtgagga ttgaaagttc ttcattaatc tttttcattt cctctttctt
     1381 ttcctttggg agattggccc cactgcgaac aaactgcttg tatgtttctt caaggagttt
     1441 tgcctgttcg ctattcagat ttagcttttc tttttgctgg taaaccgcct taaccttttc
     1501 gaaaagtttt tcgtttagat agatttcatc aaggtgaagc gaaagttttg gtgaaatctc
     1561 tttagcgata gcttgtaatt catcattggt gttggcttca gtcaggttaa aaaacacgcg
     1621 cccaacttta ttgatcaaac ttcctgaata atccagggcc tcaatggtat ttttaaaatt
     1681 gggctcttcg ttatttgaaa caatttgctc aatttccttt ttttgctgtt ctattccggt
     1741 ttcaaaagcg ggcaagtaat gtgaattgcc gattttgtca aacggaggaa caccaaatgg
     1801 ggtttgatac tcactcagaa agggattttc atcactcttt ctggcttcag tgcacgaaac
     1861 tatcgttgcc ataagtagaa tggttgaagt agttaaaaat gtaattcgca tagtatgaaa
     1921 ttttaaactt gtaaaataa
//
LOCUS       DAOPUW010000387         1939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_64240_length_1939_cov_1.887473, whole genome shotgun sequence.
ACCESSION   DAOPUW010000387 DAOPUW010000000
VERSION     DAOPUW010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64240_length_1939_cov_1.887473"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..331)
                     /locus_tag="PKJ43_06875"
     CDS             complement(14..331)
                     /locus_tag="PKJ43_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72327.1"
                     /translation="MILKEKLGERFGYFKNMGVNNVRELIQLLKKKEKLAELSKIDCF
                     SGDYLTILLRELNSTLPKPNKLTDFVEISKDTIRKLEAIRIVNTEKLYEKVIKKANIL
                     PLA"
     gene            complement(471..1412)
                     /locus_tag="PKJ43_06880"
     CDS             complement(471..1412)
                     /locus_tag="PKJ43_06880"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNZ72328.1"
                     /translation="MIEKKALYSEYVSRINRTLDYIESNIEKPMTLEELATVANFSKF
                     HFNRIFQSIVGETPFQFILRVRLERAAAFILTNKHESITEIAFKCGFSDISIFSRNFK
                     SHFQVAASKYRVNKSNNSNLSQQDSNSRQSEEQPTPYFCPELQTIKWRTNMKLNKSVE
                     VKELPKMTVAYIRHIGPYKGNDKLFESIWNRLFSWAGPRGLIGGKDFKSLVVYHDDPN
                     VTIEDKLRMSVCITVPAETKVEGEVGKMEIEAAKYVIARFELSAQDFQQAWNWVYGQW
                     FPTSGYQPDDKPCFEMYPEEPKNRKFIVDICVPVKPF"
     gene            complement(1477..>1939)
                     /locus_tag="PKJ43_06885"
     CDS             complement(1477..>1939)
                     /locus_tag="PKJ43_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNZ72329.1"
                     /translation="YDLTPSVINFYNRFEPVDLTHAAPMSSIVRNFYVTPIEVGQSVD
                     IENIYFETGKATLKPESFRSLNALIEFLNEYPNVRVEIGGHTDNVGSAEVNQRISNER
                     ALSVAEYVTTHGVPAHRVVSKGYGFDKPKASNRTAEGRAQNRRVDFTITGI"
BASE COUNT          515 a          412 c          363 g          649 t
ORIGIN      
        1 ttttaatttc tatctatgct agaggcagta tattagcttt tttaattact ttttcgtata
       61 gcttctctgt atttacaatt cttattgcct ccaacttgcg aatcgtgtct tttgaaattt
      121 ccacaaaatc agtaagttta tttggttttg gcagagtact gttgagttcc ctaagtagaa
      181 tcgttaaata atcacccgaa aagcaatcta ttttcgatag ttctgctaac ttttcctttt
      241 tcttgagtag ttgaatcaat tccctgacat tgttaacccc catattttta aaatatccaa
      301 atcgctctcc caatttttct ttgagaatca tcctgcccgg gggtaggtat tatttttcaa
      361 tttttctccg ataatcatcg atgttaaaat tttctaaatc aatataatag cccatagcaa
      421 gggggattaa aaaagagtac ctgctttgaa gcaagtactc ttagttcgta ttagaatggt
      481 tttaccggga cgcaaatgtc aacgataaat ttcctgtttt tcggttcctc agggtacatt
      541 tcaaagcaag gcttatcatc gggctgataa ccacttgtgg ggaaccactg gccataaacc
      601 caattccatg cttgttgaaa atcttgagca gaaagctcaa agcgggcaat tacatatttt
      661 gctgcctcaa tttccatttt cccaacttct ccttcaactt tagtttcagc aggaacagta
      721 atgcaaacgc tcatcctaag cttatcttcg atggttacat ttgggtcgtc atgataaaca
      781 accaacgatt tgaagtcttt gccgccaatt aaacctcttg gccctgccca tgaaaacaat
      841 ctattccaaa tgctttcgaa tagcttatcg ttccctttgt atgggccaat gtgcctaata
      901 taagcaacag tcatctttgg aagttctttc acttccacac ttttgtttaa tttcatattt
      961 gtcctccatt taatggtttg taattcagga caaaaatatg gggttggctg ctcttcgctt
     1021 tgacgactat tgctatcctg ttgacttaaa ttgctattgt ttgatttgtt taccctgtat
     1081 ttagatgccg ctacctgaaa atggctttta aaatttcggg agaatattga aatatcagaa
     1141 aacccacatt tgaaagctat ttcggtaatg ctttcatgct tattggtcag gataaatgcc
     1201 gcagcccttt ccagcctgac tctcagaata aactggaaag gggtttctcc aacaattgac
     1261 tggaaaatcc tattgaaatg gaattttgaa aaattagcta ccgtagccaa ttcttccagc
     1321 gtcataggtt tctcaatatt agactcaata tagtctaatg tccggttgat tcgggataca
     1381 tattcagaat ataaagcttt tttctcaatc accttattcc caaaattgtt gccatcttgt
     1441 ttgtaccatt gctttcaaca agtatttaca agatgtttaa atccctgtaa tggtaaaatc
     1501 aaccctacgg ttttgcgctc ttccctcggc agttcggttg ctggctttgg gcttgtcgaa
     1561 tccataaccc ttggaaacaa cccgatgtgc tggcactccg tgggttgtaa cgtactcggc
     1621 caccgacagg gctctctcgt ttgaaattct ttggttcact tcggccgaac ccacattatc
     1681 cgtatgccct ccaatctcaa cccgtacatt aggatactcg tttaggaact cgatgagtgc
     1741 gttcagtgag cggaacgatt cgggtttgag ggtagccttt ccggtttcga aatagatatt
     1801 ctcaatgtcc acggattgtc ccacctcaat gggggtaacg tagaagttgc gaacaatgct
     1861 gctcatgggg gctgcatggg tcaggtcaac cggttcgaaa cgattataga aattgataac
     1921 gctgggggta aggtcgtag
//
LOCUS       DAOPUW010000388         1933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_64515_length_1933_cov_2.790735, whole genome shotgun sequence.
ACCESSION   DAOPUW010000388 DAOPUW010000000
VERSION     DAOPUW010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64515_length_1933_cov_2.790735"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..1290)
                     /gene="recF"
                     /locus_tag="PKJ43_06890"
     CDS             complement(166..1290)
                     /gene="recF"
                     /locus_tag="PKJ43_06890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNZ72330.1"
                     /translation="MPSNENSVFLRKLTLTNFKNHTKRDFHLTKKFTAFVGQNGVGKT
                     NLLDLIYFLSLTKSFFNLTDQQLIKWNEAFSRIEAEIEKNGELLELVVKIPLNKRKEM
                     SINGAVCPKVSEYIGTLPLVIISPEDNDIIQGGSEERRKLFDNILAQVDSAYLEALME
                     YNKILEQRNSLLKIKAEKPATDISMLEFYNARLAQSGSYIFNKRKDIFQRFEAVFQQF
                     YSIISEEKEQVKWYYASQLLTKDWNTLFEQNLQKDLMLQRTSAGIHRDDYEFTIHDEK
                     IKKFGSQGQQKSFTIALKLSLYSYLHEMKGILPILLIDDLFDKLDTKRMKQLIRILQG
                     DEFGQIFMSDTDILRLEKAFDGNREQIEIFTIDLESNDKV"
     gene            1397..>1933
                     /locus_tag="PKJ43_06895"
     CDS             1397..>1933
                     /locus_tag="PKJ43_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNZ72331.1"
                     /translation="MATKLTKETYLYWYELMLLLRKFEERAGMLYGQQKIRGFCHLYI
                     GQEAVAAGMMSALQSDDRVITAYRDHALAIAKGISCNAAMAELYGKETGCTKGKGGSM
                     HFFSKEHNFFGGHGIVGAQIGLGAGIAFADKYLDKSNVTICFFGDGAARQGILHETFN
                     MAMVWKLPVIFVCENNGYA"
BASE COUNT          536 a          365 c          378 g          654 t
ORIGIN      
        1 gtgataaaca attttcggtc cttaagtaca attgtctttg tgtgttttga aataaggcca
       61 cccatcagcg tgtcccatag tgatgcaatc ttggattggg caatggattc ttctaatcca
      121 ctttccctga tccacatctt tatggcatcg cctaaactga tgtcgttata ccttatcatt
      181 cgattctaaa tctatggtaa aaatctcaat ttgttctcta tttccgtcaa aagctttttc
      241 aagtcgcaaa atatcggtat ccgacataaa tatctgtcca aattcatctc cttgtagtat
      301 acgtattaat tgcttcattc gtttcgtgtc gagtttgtcg aacaaatcgt caattagtaa
      361 tataggcaat atgcctttca tttcatgtaa atagctgtag agcgaaagtt ttaacgcaat
      421 ggtgaacgat ttttgttgcc cttgcgaacc aaatttctta atcttttcat cgtgaatggt
      481 aaactcataa tcatcgcgat gaatccctgc tgatgtgcgt tgtaacatta aatccttttg
      541 taagttttgt tcaaaaaggg tgttccaatc tttggttaat aattgagaag cataatacca
      601 tttcacctgt tctttttctt cactgataat ggagtaaaac tgttgaaata cagcttcaaa
      661 ccgttggaaa atatcttttc gtttgttgaa aatatagctt cctgattgtg ctaagcgcgc
      721 attgtaaaac tcaagcatac tgatatcggt agccggtttt tcggctttta tctttagtag
      781 cgaattgcgt tgctccaaaa ttttgttgta ctccattagc gcttccaaat aagcactgtc
      841 tacttgtgcc aaaatattgt caaacagttt tctgcgttct tcgctgccac cttgaataat
      901 atcattgtct tcaggcgaga taatcaccaa tggtaatgtg ccgatatact ccgaaacttt
      961 cgggcaaaca gctccattta tactcatctc ttttcgtttg ttcagcggga tttttaccac
     1021 caattccagc agctctccgt ttttttcgat ttcagcttcg attctggaaa atgcctcgtt
     1081 ccacttaatg agttgctgat ctgtaaggtt gaaaaagctt ttagtgagac tcaaaaagta
     1141 gattaaatcc agcaaattcg ttttacccac accgttttgc cccacaaaag cggtaaactt
     1201 tttcgtgagg tgaaaatcac gtttagtgtg atttttgaag ttggtgagcg ttagttttct
     1261 tagaaaaacg ctattttcat tggatggcat gaattcccgg aattattgca ccgaaaccta
     1321 tgtaaaattt tcattgtagg gtataaaact taaaataaaa aaaaatactt aggtttgtcc
     1381 gctttattaa aaagttatgg ccacaaaatt aactaaagaa acatacctct attggtatga
     1441 actgatgctt cttcttcgca agttcgagga gcgtgcgggt atgctttacg gccaacagaa
     1501 gattcgtgga ttctgtcatt tgtacattgg acaagaagct gttgcagcag gaatgatgtc
     1561 ggcattacag tcggatgatc gagtaattac cgcctatcgc gatcatgcat tagctattgc
     1621 caaagggatt agttgtaatg cagctatggc tgagttatac ggcaaggaaa ccggttgcac
     1681 caaaggaaaa ggaggctcaa tgcacttttt ctcgaaagag cataattttt ttggcggaca
     1741 tggaattgtg ggtgcacaaa ttggcttagg agccggaatt gcatttgccg ataaatatct
     1801 cgataaatcg aatgttacca tttgcttttt tggcgatggt gctgctcgtc agggtatttt
     1861 gcacgaaacc tttaatatgg ctatggtttg gaaattgccg gttattttcg tttgtgaaaa
     1921 caatggctat gcc
//
LOCUS       DAOPUW010000389         1932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_64553_length_1932_cov_3.385189, whole genome shotgun sequence.
ACCESSION   DAOPUW010000389 DAOPUW010000000
VERSION     DAOPUW010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64553_length_1932_cov_3.385189"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..1149
                     /locus_tag="PKJ43_06900"
     CDS             235..1149
                     /locus_tag="PKJ43_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72332.1"
                     /translation="MQKIVSIILLSFFVAIAGNAVAQLVEQRSVAPMSAFSPLEPASY
                     ILLGEGYGNIEPLVYEVGFAPQFRIAANRFPNFGFIFTPNFVLRMRDQFSHPIRTPSY
                     MPKGTLFYHVGNDEVTGVDRFAFLTIGHHSNGQEGSFFEADSQTINTFNGSFSSNYAS
                     LGYEWISPNKRTFNPASNFRLSSTFYFVNKIIRSMYGPLRFQADLQSTLYLTGEKNNF
                     FRPEKSKAALVGKMNVTWLAMGVKDAEPIDLKRLVFSYTLSYQPSFISDVAFFTRFYY
                     GQDYYNINFERTLKVLQFGFSVRNISFR"
     gene            1257..>1932
                     /locus_tag="PKJ43_06905"
     CDS             1257..>1932
                     /locus_tag="PKJ43_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNZ72333.1"
                     /translation="MKTRSLKKFIYLSIAAAVITIVLKVIAWYITDSVGLLSDAMESC
                     VNLIAAVIALVMINIAEKPADHEHAFGHGKAEYFSSAIEGAMIIIAAFSIIWSAVPRL
                     LHPQSIENAGIGLMVSVFASLINLAVGLILIRNGRANNSITLEADGKHLMTDVYTSIG
                     VLLGILLVKLTGWLILDPIVAVGVALNILYTGYHLIQRSATGLLDCALSDDEITRIGQ
                     VMKDYQE"
BASE COUNT          523 a          354 c          405 g          650 t
ORIGIN      
        1 ttactgttta caagcggtct gaactcaaaa ataaagtcga actcaggtta ataaaaagta
       61 aataaacact ttgcattttc ttttttaggc tcatcactca ggaaatcctt gagatgtaaa
      121 atttttgtcg aaacttgaat gaaacgtttc agatataaaa ttgaagcttt atttttgcga
      181 cctatatttt acacaaaatc acaacgttag tttacgacaa agttttttat catcatgcaa
      241 aaaattgtat cgattattct cctgtctttt tttgttgcta ttgctggtaa tgctgtggca
      301 caattagttg aacagcgttc ggtggcacca atgagtgcgt tttcacccct tgaaccagcg
      361 agctatatct tgctgggtga aggctatgga aatatagaac cgttggtata tgaggtgggt
      421 tttgcacctc agtttcgtat tgcggccaat cgtttcccaa actttgggtt tattttcact
      481 ccgaatttcg tattgagaat gcgggatcag ttttcgcatc ctattcgtac tccgtcgtat
      541 atgcctaaag gaacgttgtt ttatcatgtg ggaaatgatg aggttactgg tgtcgatcgg
      601 tttgcctttc tcactattgg gcatcactct aacgggcagg aaggttcctt tttcgaagcg
      661 gatagccaaa ccatcaatac cttcaacggg agtttttctt ccaattatgc ttcgttgggc
      721 tacgagtgga taagcccgaa caagcgcacg tttaacccgg ccagtaattt caggctttct
      781 tctacctttt attttgtaaa taaaattata cggagcatgt atgggccgtt gcgctttcag
      841 gctgatttgc aatctacttt ataccttacc ggcgaaaaga ataatttctt tcgtcccgag
      901 aaaagcaagg cagcattggt aggtaaaatg aatgttacat ggttggctat gggcgtgaag
      961 gatgccgaac cgattgactt gaaacgattg gtttttagct atactctttc ttatcagcca
     1021 tcttttatca gcgatgtagc tttttttacc cgtttctatt acgggcagga ttattacaat
     1081 atcaatttcg agcgtacctt aaaagtgcta caatttggtt tttcggttcg aaatatctca
     1141 ttccgctgat atgcaaaaaa ggtaatataa ttttcgctga agacaatctt gtttatcttt
     1201 gttttatgaa atcaaattac tgtaaatcag tataatttat tcaatgaatt aaagaaatga
     1261 aaacccggtc gctgaaaaaa ttcatctatc tgtcgatagc tgcagcagtt ataacaattg
     1321 tactcaaggt tattgcttgg tacataaccg attcggtggg attgttgtct gatgccatgg
     1381 aatcttgcgt aaacctaatt gcggctgtta ttgcccttgt aatgattaat atagctgaaa
     1441 aaccagccga tcatgagcat gctttcggcc acgggaaagc agaatatttt tccagtgcca
     1501 ttgaaggagc tatgattatt atagctgctt ttagtattat ttggtcggca gtgccgcgtt
     1561 tattacatcc acagtcaatc gaaaatgcag gaattgggtt aatggtgtcg gtttttgcat
     1621 cgcttataaa tcttgctgta ggccttatac tcattcgcaa tgggcgggca aacaattcga
     1681 taacccttga agcagacggg aagcatttaa tgaccgacgt ttatacttca ataggtgtat
     1741 tgcttggtat ccttttggtg aaattgactg gttggctaat attagacccg attgttgctg
     1801 tgggggttgc attaaatatc ctttataccg gatatcattt aattcaacgt tcagccactg
     1861 gtttgcttga ttgcgctctt tctgatgatg aaatcactcg cattgggcag gtcatgaaag
     1921 attatcagga gc
//
LOCUS       DAOPUW010000390         1930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_64673_length_1930_cov_3.037867, whole genome shotgun sequence.
ACCESSION   DAOPUW010000390 DAOPUW010000000
VERSION     DAOPUW010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64673_length_1930_cov_3.037867"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..164
                     /locus_tag="PKJ43_06910"
     CDS             <1..164
                     /locus_tag="PKJ43_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HNZ72334.1"
                     /translation="QGGQWKAVFIDHGYLTPEMLSIDYLRWFYTAFTRPKEKLYLVNF
                     DKRFFGNED"
     gene            complement(192..1127)
                     /locus_tag="PKJ43_06915"
     CDS             complement(192..1127)
                     /locus_tag="PKJ43_06915"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNZ72335.1"
                     /translation="MIPFELTILGTSSALPTLKRYPTAHALNVRERFFLIDCGEGTQI
                     QIRKYGVKLSRIDHIFISHLHGDHYFGLIGLLSTFVLLGRKNGLHIFSHSQLPEILKP
                     QLDFLNEDLSYKIIWHPLNFKKNDIILDNEVVTVMSFPVKHRVPCCGFIFSEKPGLLN
                     LQKDKIEEYKVPVSWLQYIKKGEDYVDENGLVVPNSALTCQKHLPRRYAFCTDTSYVP
                     ELSEIFENVDVLYHEATYDSRYEKRANETFHSTAAQAANMANSSRVGQLIIGHFSARY
                     KDVTVLHDEAKSIFPNTLIAEEGMVISIEGRKEIL"
     gene            complement(1145..1495)
                     /locus_tag="PKJ43_06920"
     CDS             complement(1145..1495)
                     /locus_tag="PKJ43_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNZ72336.1"
                     /translation="MDYKVDKKEKYVVVTTTSEKLNTTNAPDLKSEFVMLTNQGVGNI
                     ILDLSSCEYCDSSGLSAILVGNRLCEQVNGKFVITGLQQEVESLIRISLLDTILNIKD
                     SLDAAESVMEYDPE"
     gene            complement(1545..>1930)
                     /locus_tag="PKJ43_06925"
     CDS             complement(1545..>1930)
                     /locus_tag="PKJ43_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNZ72337.1"
                     /translation="TVDSIHEGTIVELFDKGAVIALPYGVEGFATPRHLTKEDNSAVN
                     VEDKLPFKVIEFSKTAKRIILSHSRVWEDVKRAEEKETKKTASKQSAKSVKNVNDNVE
                     KTTLGDISELAALKDQMENAEKDKK"
BASE COUNT          597 a          384 c          351 g          598 t
ORIGIN      
        1 cgcaaggggg tcagtggaaa gctgtattta tcgatcatgg atatttaacg cccgaaatgc
       61 tttcaattga ctatttgcga tggttttata cagcttttac acgaccaaaa gaaaaattgt
      121 atctggttaa cttcgacaaa cgtttcttcg ggaacgaaga ttgatgaaaa taaaggataa
      181 aactcaaaat atcagagaat ctctttgcgc ccctcaattg agattaccat tccttcttcg
      241 gcaataagag tattgggaaa tatagatttt gcttcgtcat gtaagacagt cacatcttta
      301 taacgcgctg aaaagtgacc aattatgagc tgccccaccc tgctactatt tgccatattg
      361 gcagcctgtg cagctgttga atggaaggtt tcattggcac gtttttcata acggctatcg
      421 taggttgctt cgtgatacaa cacatcgaca ttctcgaata tttcagaaag ctcggggaca
      481 tatgaagtat ctgtacaaaa cgcataacgg cgcggtaaat gcttctggca agtcaatgct
      541 gaatttggaa cgacaagtcc gttttcatcg acataatctt cgcctttctt tatgtattgc
      601 aaccaactaa cagggacttt gtactcttca attttatcct tttgcaaatt gagcaagcca
      661 ggtttttctg aaaatataaa accacaacaa ggcacacggt gttttacagg gaacgacata
      721 acagtaacta cttcgttatc gagaatgata tcgttctttt taaaattcaa cggatgccaa
      781 ataatcttat aagaaagatc ttcatttaaa aaatcgagct gaggcttaag aatctctggc
      841 aattgagaat gagaaaaaat gtgaaggcca tttttccggc caagtaaaac aaaggttgaa
      901 agtaaaccaa tgaggccgaa gtaatggtct ccatgcaaat gcgaaataaa aatatggtcg
      961 atgcgcgata atttcacccc atattttctg atttgaattt gggtaccttc accgcaatcg
     1021 attaaaaaaa accgctcacg cacattaagt gcatgagcgg taggatatct tttcaaagtt
     1081 ggaagcgctg agcttgtacc taatatagtc aattcgaaag gaatcataca gtccaagtaa
     1141 gtttttactc ggggtcgtac tccataacag attcagcagc gtctaaacta tctttaatgt
     1201 tcagaattgt atctaggagc gaaatcctga tcaaactttc aacttcttgt tgtaaccctg
     1261 taattacaaa ttttccattg acctgctcac acagcctgtt tcccaccaga attgcactta
     1321 gcccagatga atcgcaatat tcgcaactcg ataagtcgag aataatatta ccaacacctt
     1381 gattcgtgag catcacaaat tctgatttca gatctggagc atttgttgtg tttagcttct
     1441 cagaagttgt tgtaacaaca acatattttt ctttcttgtc aactttataa tccatagcaa
     1501 aagcaaggta caaaatttcg atgtaaaaaa actatactaa agatttattt tttatccttt
     1561 tcagcatttt ccatctgatc tttgagagct gcaagttcag aaatatcgcc caaggttgtt
     1621 ttttcaacat tgtcgtttac attcttcact gattttgcag attgctttga agcggtcttt
     1681 ttggtttcct tttcttcagc acgtttaaca tcttcccata cacgtgagtg cgaaaggatt
     1741 atgcgttttg ctgttttcga gaattcaata accttaaacg gcaacttgtc ttcaacattc
     1801 actgcagaat tatcttcttt agttaaatga cgaggagttg caaaaccttc aacaccataa
     1861 ggcagagcaa ttacagctcc tttgtcgaaa agctcaacaa tggttccttc gtgaattgaa
     1921 tcaacggtaa
//
LOCUS       DAOPUW010000391         1930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_64676_length_1930_cov_2.955733, whole genome shotgun sequence.
ACCESSION   DAOPUW010000391 DAOPUW010000000
VERSION     DAOPUW010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64676_length_1930_cov_2.955733"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1930)
                     /locus_tag="PKJ43_06930"
     CDS             complement(<1..>1930)
                     /locus_tag="PKJ43_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNZ72338.1"
                     /translation="IIVDEAHTCTKNLDFKKVSQSQQRYELLEKIAKNDKQHLLLLTA
                     TPHSGKDGEFKSLLGLVNAEFENYDFSDLTTSEKRKVATHFIQRKRNNIEKWLDEETP
                     FPKRTMEELPYTLSASSDYYKLYQEVLKFARGISTEGITENKARIKYFAALSLLRGVM
                     SSPLAGFEMLQKRKAKIAENGEITDEEVKDNPVVERWDEQSDSEQIEIIERADLSDSE
                     WSTLNRLSLKAQELTNIEKDQKVKRAVEQIKLWINKGHSPIVFCRFIATAQYVAKILK
                     ENLPANIDVRAITSELSDEERREKIDEMAKSPKRILVATDCLSEGINLQDKFNAILHY
                     DLPWNPNRLEQRTGRVDRFGQLGWIDKNGQYQNTIDVKVLFGEDNPIDVVVLKIIIEK
                     IQRIQISSGVSISLADDNRSVMDKVLREVLLNPEKAQNRFSKQMRIDFGPSPELDELD
                     IEITNEIESAKEKAEKIQSIFAHESIMPEDVKKDLHEVDEAIGDVTTLEAFVLAAGQL
                     LGARFEPVEGGYIFKKTNMDDWLAAALGQGDRIHISFLSPTPLGFRYIGRNHRFVEQL
                     CHRIIANSLDNEKKGQKAARASVFRTDAVQMQTTLIQFRVRNVIREVGKLHEMVSEEM
                     FLWGYEQTPDGIKTLDVEV"
BASE COUNT          498 a          438 c          351 g          643 t
ORIGIN      
        1 aaacttcaac atctaaggtt ttgatgccat caggcgtttg ctcgtaaccc cataaaaaca
       61 tttcttcgga aaccatttca tgcagtttgc ctacttcacg aattacattc ctgaccctga
      121 actgaattaa ggttgtttgc atttgaacgg catctgttct gaaaacagaa gctcttgcgg
      181 ctttctgtcc tttcttttcg ttatccaaag aattggcaat gatacgatgg caaagttgct
      241 ctacaaatcg gtgattacgc ccgatataac ggaagcccaa tggtgtagga gacaggaaag
      301 aaatatgtat tctgtcgccc tgccccaagg cagcagctaa ccagtcgtcc atgttcgttt
      361 ttttaaaaat ataaccgcct tccacaggtt caaatcttgc ccctaatagt tggcctgctg
      421 ccaaaacaaa agcttctaac gtggttacat caccaatggc ttcatccact tcatgcaagt
      481 ctttttttac atcttcgggc atgatgcttt cgtgggcaaa aatggactgg attttttcgg
      541 ctttttcctt tgccgattca atttcgtttg tgatctctat atccaattca tccaactcgg
      601 gactgggacc aaaatctatg cgcatttgct ttgagaaacg attctgggct ttttcagggt
      661 tcaacaagac ttctcttagt actttatcca taacagaacg gttatcgtca gccaatgaaa
      721 tgctgacccc tgatgaaatc tgaatgcgtt gaattttttc gatgataatc ttcaaaacaa
      781 ctacatcaat cggattgtct tcgccaaaca gcacctttac atcaatggta ttttggtatt
      841 gcccgttttt gtcaatccaa cccagctgac cgaaacgatc gaccctgcct gttctttgtt
      901 cgaggcggtt aggattccag ggtaaatcgt aatgcaggat ggcattgaat ttatcctgca
      961 ggttgatacc ttcactcaaa caatctgtag ctaccaaaat acgtttcgga cttttggcca
     1021 tttcatcaat tttctccctg cgctcttcat cactcagttc gctggtgatt gcccggacat
     1081 caatattggc gggtaaattc tctttaagaa ttttagcaac atactgcgcc gttgcaataa
     1141 aacggcagaa cactatcggg ctatgacctt tgtttatcca tagtttgatt tgttccaccg
     1201 cccttttaac cttctggtct ttttcaatgt ttgtcagctc ctgtgctttt agcgataaac
     1261 ggttgagcgt actccactca ctatctgaaa gatcagcacg ttcaattatc tcaatttgtt
     1321 cggaatcgct ttgttcatcc catctttcaa caacaggatt atcttttact tcttcatcag
     1381 tgatttcgcc attttctgca atcttagctt ttcttttttg cagcatttca aaacctgcca
     1441 aggggctact cataacgccc ctgagcaaag acagtgcggc aaaatatttt atacgggctt
     1501 tgttctctgt aatgccttca gtactgatac cccgtgcaaa ctttaacacc tcctgatata
     1561 atttataata atcagaggaa gcagataagg tatatggcaa ttcttccata gtcctcttgg
     1621 gaaacggagt ttcttcgtcg agccattttt caatattatt tcttttccgc tgaatgaaat
     1681 gagttgctac ttttcttttt tcagaagttg tcaggtcgga aaaatcataa ttttcgaatt
     1741 ctgcatttac caaacctaat aaggatttaa actctccgtc tttaccgcta tgcggggtgg
     1801 cagtgagtaa aagcaaatgc tgtttgtcat ttttagcaat cttttcaagt aactcataac
     1861 gttgctggct ctggcttact tttttaaagt caagattctt tgtacaggtg tgtgcttcat
     1921 caacaatgat
//
LOCUS       DAOPUW010000392         1916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_65366_length_1916_cov_2.990865, whole genome shotgun sequence.
ACCESSION   DAOPUW010000392 DAOPUW010000000
VERSION     DAOPUW010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65366_length_1916_cov_2.990865"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="PKJ43_06935"
     CDS             <1..1025
                     /locus_tag="PKJ43_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ72339.1"
                     /translation="ILGTGSGTYYDAISQTWKKSYGVVQNANPDLKWESTSQINIGLD
                     FAVLGRINGTLELYQKNTSDLLYTYAVPQPPYLVGTMLANVGDLSNKGIELSLDASLI
                     EKKDLRWNINLTMAKNIQVVEKLSNQAYQCDSINSGSLHGLTGMSGKYSQVIKEGYAV
                     GTFVGPVSTGLDSLGKFIFENNAAQQIIGDVQPKLSIGLGTSLSYKNIDFEISTYGML
                     GQKVLNATAMELSNPKRLPTNNVSDIILSSEITSPMTYSSYWVEDASFLRIQSITLGY
                     TLKAASIGIEKIRFYVTGENLFVFTNYTGIDPEVSIDGLDSPGIDWFNAYPRPRTFSF
                     GINVTF"
     gene            1044..>1916
                     /locus_tag="PKJ43_06940"
     CDS             1044..>1916
                     /locus_tag="PKJ43_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNZ72340.1"
                     /translation="MRNYKNTILTLVLAAIFAGCTNLDETLYDKVKSSDYGKTSGEIE
                     TIVGSAYATLRGFGDNISLSYPACEFLLFCNETTSDEVCIPTRGSDWYDNGRYQQAEL
                     HTWGNDNLLILSSWRYCYEGISKVNAVIYQVKKSELSEEEKNVVYAELRGIRAYYYSI
                     LLDMFGNVPLITNFEDTSLPKNSTRAEVYAFVESELLDIMELLPSEIIYGRITQNVAN
                     ALLARLYINSEVYIGQARWQDCIDACDKISGYVLEGNYFANFLTGNEGSRENIFAIPF
                     DYTKQGTVGNYLIAL"
BASE COUNT          601 a          398 c          404 g          513 t
ORIGIN      
        1 atatccttgg aacaggatca ggcacttatt atgatgcgat ttctcaaacc tggaaaaaat
       61 cgtatggtgt ggttcaaaat gccaatcccg accttaaatg ggaatcgact tcacaaatca
      121 acattggact cgattttgcc gttcttggtc gcattaatgg gacactggaa ttataccaaa
      181 agaacacgag cgatctgctt tatacctatg cagtgccaca accgccgtac cttgtgggca
      241 ccatgctggc caatgttgga gatttgagca acaaagggat agaactttcg ctcgatgcca
      301 gccttatcga gaaaaaagat ttgcgttgga atataaacct cacgatggca aagaatattc
      361 aagtagttga aaaactctcg aaccaagcct atcaatgcga ttcaatcaat agtggttcac
      421 ttcacggatt aaccggtatg tcgggaaaat attcgcaggt tataaaagaa ggctacgctg
      481 ttggaacctt cgtgggacca gtcagcacag gattagattc gctgggtaaa ttcattttcg
      541 aaaataacgc tgctcagcaa attattggcg atgtccagcc gaaactaagc attggcctgg
      601 gaaccagctt gtcgtacaaa aacatcgatt ttgaaatatc tacgtatgga atgttgggac
      661 aaaaagtttt gaatgccacc gcaatggaac ttagcaatcc gaaacgttta cccaccaata
      721 acgtatcgga cataattctt tcatcagaaa ttacatctcc aatgacgtac tcaagttatt
      781 gggtcgaaga tgcttctttc ttgcgaattc agagcatcac cttgggttac acgctaaaag
      841 cagcgagcat tggcatcgaa aaaatcagat tctatgttac tggtgaaaat ctttttgtat
      901 tcactaatta cacgggtatt gacccagaag taagcattga cggactggat tctccgggga
      961 tcgattggtt caatgcctat cctcgtccaa gaacattttc gttcggaata aatgtaacct
     1021 tttaacctta atgctaaagt attatgagaa attacaaaaa tacaatactg acactggtac
     1081 ttgccgcaat atttgccggt tgtacaaatc tggacgaaac actttacgac aaggtaaagt
     1141 catcggatta cgggaaaaca tcaggagaaa ttgaaaccat tgtaggaagc gcttatgcta
     1201 cgctccgcgg atttggagat aacatttcat taagctatcc ggcatgtgaa ttccttctct
     1261 tttgcaacga aaccacttca gacgaagtgt gtatacctac ccggggaagc gactggtatg
     1321 acaatggccg ctaccagcaa gccgaattac acacatgggg caacgataac cttctaattt
     1381 taagctcctg gagatattgt tacgaaggca tcagcaaagt taatgcagta atatatcaag
     1441 ttaaaaaatc ggaactcagc gaagaagaaa aaaatgttgt ctatgccgaa ttaaggggaa
     1501 tcagggcata ctattattcg attcttctcg atatgtttgg caatgtgccg cttataacca
     1561 atttcgaaga cacgagctta cccaaaaatt cgacccgcgc cgaagtatat gctttcgttg
     1621 aatcagaatt gctcgacatt atggagttac tgccttcaga aattatttac ggacgtatta
     1681 ctcagaatgt agcaaacgca ttgctggcaa gactgtatat aaattcagaa gtatacatcg
     1741 gtcaggctcg ctggcaagat tgtatcgatg cctgtgataa aatctcgggg tacgtgctcg
     1801 aaggcaacta ctttgccaac ttccttaccg gaaacgaagg ttcgagagag aacatcttcg
     1861 ccattccgtt tgattataca aaacaaggaa ccgtaggtaa ttatctgatt gccctc
//
LOCUS       DAOPUW010000393         1912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_65621_length_1912_cov_2.430264, whole genome shotgun sequence.
ACCESSION   DAOPUW010000393 DAOPUW010000000
VERSION     DAOPUW010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65621_length_1912_cov_2.430264"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="PKJ43_06945"
     CDS             complement(<1..802)
                     /locus_tag="PKJ43_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNZ72341.1"
                     /translation="MIEYKKHTLANGLRVIVHTDKSTPMTAVNVLYNVGSKDENPEKT
                     GFAHLFEHLMFGGSKHIADYDTPLQKVGGENNAFTTNDLTNYYLTLPSRNIETGLWLE
                     SDRMLELAFNKKSLETQRSVVIEEFRQRYLNQPYGDAMALLYEMAYKVHPYRWPTIGR
                     DISHIENATMEDVKEFFLKFYTPNNAVLSICGNIEADEAFALSEKWFGSIPSRNVPIR
                     NLPQEPLQAERREQSVTRSVPYNALNMAFHMSSKFTPEYYASDLISDIL"
     gene            complement(961..>1912)
                     /locus_tag="PKJ43_06950"
     CDS             complement(961..>1912)
                     /locus_tag="PKJ43_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNZ72342.1"
                     /translation="AKEIEIDAVAHNGEVVAYAISEHVEFAGVHSGDATMVFPPQKLY
                     IETIRRIKKIARQVAKGLEISGPFNMQLLAKDNDIKVIECNLRASRSFPFVSKVMKFN
                     MIELATKVMLGVPYKKPDKSLFELDHVGVKAPQFSFHRLLNADPVLGVDMSSTGEVAC
                     LGDNYYEAIMKSMLSVGYRIPEKNILISSGPARSKMELLNSAKTLVEKGYNIYASDGT
                     YRYFRDNNIACKLLHWPDEEESKPNISDYIKEKKIDLVINIPKDHTTRELGNGYWIRR
                     NAIDYNIPLITNARVASAFIQAFCKYKFEDLAIKSWDEYK"
BASE COUNT          475 a          394 c          430 g          613 t
ORIGIN      
        1 aaagaatgtc tgaaataagg tcactggcat aatattccgg ggtaaatttg ctgctcatgt
       61 gaaaagccat attgagcgcg ttgtatggaa cacttcgtgt tacactttgt tcacggcgtt
      121 cagcttgcag tggttcctgg ggaaggtttc ttatcgggac gtttcgagag gggattgatc
      181 caaaccattt ttctgaaagg gcaaatgctt catcggcttc aatgttgcca catattgaaa
      241 gcacggcatt gtttggtgta taaaatttta aaaagaactc tttgacatct tccatggttg
      301 cattttcgat gtgcgaaata tcgcggccga tggttggcca ccgatacgga tgaaccttgt
      361 aggccatttc gtaaagcagg gccatggcat cgccataagg ctggttgagg tagcgctgcc
      421 tgaattcttc aattactaca cttcgctggg tctcaagact ttttttgttg aaagccagtt
      481 ccagcatccg atccgattca agccataatc ccgtttcgat atttcgtgac ggcagggtta
      541 aatagtagtt ggtcaggtcg tttgtggtaa atgcattgtt ctctccacca actttttgca
      601 agggcgtgtc atagtcggct atgtgcttcg accctccaaa catgaggtgc tcaaataaat
      661 gggcaaagcc tgttttttca ggattctcat ctttcgaccc tacgttataa agtacattta
      721 ctgcggtcat tggggtcgat ttgtcggtgt ggacaattac ccgaagtcca tttgcgagtg
      781 tatgtttctt gtattcaatc atttttacaa agcatatatt gaagttatcg ggggcggatt
      841 taaaaggcga atgtacgtta aaattgttta tcgcccgcac cctgtttttt aagaaaaaga
      901 gaaccgcctt cattgagttt tgtttcaatg aaggcggtta tgaaaaaccc ccaaacaatg
      961 ttatttatat tcgtcccagc tcttaatggc cagatcttca aatttatatt tgcagaatgc
     1021 ctgtatgaat gcacttgcaa cgcgtgcgtt ggtgatcagc gggatgttat aatctatggc
     1081 atttctcctg atccagtacc cgttacccag ctcgcgtgtg gtgtggtctt tagggatatt
     1141 tattaccaaa tcgattttct tttctttgat gtaatcgctg atgtttggtt tcgactcttc
     1201 ttcatcgggc cagtgaagta gcttgcatgc tatgttattg tctctgaaat agcggtaagt
     1261 gccgtccgat gcataaatgt tgtacccttt ttctaccagt gttttagcag agttgaggag
     1321 ctccattttc gatcgtgcag gccccgatga gataagaatg tttttctctg gaattctata
     1381 gcccaccgat aacattgatt tcatgattgc ttcgtaataa ttatcgccaa gacaagccac
     1441 ttcgcctgtt gaactcatgt caacaccaag aacagggtcg gcatttagca aacggtggaa
     1501 cgaaaactga ggagctttta ctcccacatg gtcgagctcg aaaagcgatt tgtccggttt
     1561 tttataagga accccgagca ttacttttgt ggctaattct atcatgttga atttcatcac
     1621 cttcgataca aaggggaaac tcctcgatgc tcttaggtta cattcaatta ctttgatatc
     1681 attgtccttt gctaaaagtt gcatgttaaa gggaccagaa atttcaagtc ctttagctac
     1741 ctggcgggcg atctttttaa tccttctgat ggtttcaatg tagagttttt gaggcgggaa
     1801 aaccatggtg gcgtcgccgg agtgaacccc tgcaaactca acgtgttctg aaattgcgta
     1861 ggcaacaact tcaccattgt gcgcaactgc atcaatttca atttctttgg ct
//
LOCUS       DAOPUW010000394         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_65704_length_1910_cov_2.421024, whole genome shotgun sequence.
ACCESSION   DAOPUW010000394 DAOPUW010000000
VERSION     DAOPUW010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65704_length_1910_cov_2.421024"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1845)
                     /locus_tag="PKJ43_06955"
     CDS             complement(<1..1845)
                     /locus_tag="PKJ43_06955"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNZ72343.1"
                     /translation="MPLQFKSKDQSYNTPTGIKNLKDAVSSTSTVAANDLQFSSASSF
                     DNTLQGLVPGLNIVSSSGMPGTMSWINMGGVSSMNCRNEPLLLIDGMIFDYNYASEGV
                     LEGFSLNPLDIIDYQDISDVSAIKSGNGYFGSLSSNGLIYVNTEQKSEASTLIQISAY
                     GGIGLMPKTLDVMDAQQFRQYFSGRLAEEGLSSSQIDQTYPWLNGQPGSSEYYRHNHD
                     TKWQNEILTPGALSKFHIFLKGGDDIATYNISSGYVSQNSIYDGAKYSRFNLKVNGKI
                     NITDKFSITPNVKLSLADSYTPNQGPSLYKNPLISSLLIPSILNPYATDEKNGELLYY
                     FDNESSFNVSNPKAIVTNAQGTNRNYNFLSSVHAQYKISETISLNDMLGINFNNAREN
                     VFLPDLGLVQIDSAANSPQVYINEFRSTQNYATIDYNKRTKSGHSINGQAGMRLIMNS
                     YKTNKAISLNTPSDDFKTLGNGAKYNYLRTSTGGTQKLNVVSYFATVDYSFRSKYYVN
                     VNASYDGNSAIYENNRYNLYSSAGLAWRLSSEKFLSGQKWLSDLKLRANWSTGGNVYS
                     SIYEYSKLYYGEERVDAYGVPVRESVPNPDLEIERKTTINGGIDISLFK"
BASE COUNT          515 a          395 c          400 g          600 t
ORIGIN      
        1 tttgaacaac gagatgtcga tacctccatt aattgttgtt ttccgttcaa tttcaagatc
       61 aggatttgga acagattccc tcaccggaac accataggca tccactcgtt cttcaccgta
      121 gtaaagtttt gagtattcgt agatgctgct ataaacattc ccacctgttg accaattggc
      181 acggagtttc aaatcgctga gccatttttg tcctgaaagg aatttttcgg aagagagcct
      241 ccatgctaag ccagccgaag agtacaagtt gtaccggtta ttttcgtaga tcgccgaatt
      301 tccatcatac gatgcattaa cattcacata gtatttgctg cggaaactat aatcaacggt
      361 agcaaagtac gaaacgacat taagcttctg ggttcccccg gttgatgttc gtaaatagtt
      421 gtatttagca ccgtttccca aggttttgaa atcgtcggaa ggtgtattca aactaatggc
      481 tttgtttgtt ttgtagctgt tcatgatgag cctcatacca gcttgcccgt taatagaatg
      541 ccccgatttg gttctcttgt tgtaatcgat ggtcgcgtaa ttttgagtcg acctgaattc
      601 gttgatgtaa acctgaggac tatttgctgc agagtcgatt tgtaccaagc ccaagtcggg
      661 aaggaaaacg ttttccctgg cattgttgaa attgataccc agcatgtcgt tcagactaat
      721 tgtttccgag attttgtatt gcgcatgtac tgaactcaag aaattgtaat ttctgttggt
      781 accttgcgcg ttggttacaa tggctttcgg gttactcaca ttgaaagacg attcgttgtc
      841 aaaatagtat aacaattcgc cgttcttttc gtcggttgca taaggattca atatcgacgg
      901 gataagcaag ctcgaaatca acggattctt atacaaacta ggtccttggt tgggtgtgta
      961 actatcagca agcgataatt ttacgttagg tgtaattgaa aatttgtcgg taatattgat
     1021 tttcccgttg actttcaaat tgaaccgtga atacttagcc ccatcgtaaa tactattttg
     1081 gcttacgtac cccgatgaaa tattatatgt tgcaatatcg tcacccccct ttaggaagat
     1141 gtgaaatttg ctcaacgcac ctggagtaag aatctcgttt tgccatttgg tatcatggtt
     1201 atgcctgtaa tattcactac tgcctggttg cccgtttaac catggatatg tttggtcaat
     1261 ctgagaactg cttaagcctt cttcggccaa gcggcccgag aaatattgcc tgaattgctg
     1321 tgcgtccatt acatcgagcg ttttgggcat taagccaatt cctccgtatg ccgaaatctg
     1381 gataagcgta ctagcttcac tcttctgttc tgtattcaca tagataaggc catttgagct
     1441 taaggatcca aaatatccgt tgccgctttt gatggccgaa acatcggaaa tatcctgata
     1501 atctataata tccagagggt tgagagaaaa accttccaaa accccttctg aagcgtagtt
     1561 gtaatcaaaa atcatcccgt caattaagag aaggggttcg tttctacaat tcatagaaga
     1621 aactccgccc atgttgatcc acgacattgt tccaggcatt cccgacgaag agacaatatt
     1681 caacccggga acaagtccct gaagcgtatt gtcgaaagaa gaagcgctgc taaattgcag
     1741 atcgtttgct gcgactgttg aagttgacga tactgcatct ttgaggttct ttattcctgt
     1801 aggagtgtta tacgactggt ctttcgactt gaattgtaag ggcaccaaaa aaactgtcaa
     1861 tttatcattt cccgataaat agatattttg ttttacgtat cctggcaagt
//
LOCUS       DAOPUW010000395         1908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_65787_length_1908_cov_2.683216, whole genome shotgun sequence.
ACCESSION   DAOPUW010000395 DAOPUW010000000
VERSION     DAOPUW010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65787_length_1908_cov_2.683216"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..1255
                     /locus_tag="PKJ43_06960"
     CDS             14..1255
                     /locus_tag="PKJ43_06960"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNZ72344.1"
                     /translation="MKIADHINIPHTEEMVLSNGIKVYGIPNDQGVIKVDFVFDAGKW
                     FEPKNLVADFANKLMREGTKQKSSLAINEILDFYGCNLEEQSYFSNAGFQLYSLSRNI
                     HQVLPLMKEMLTEAAFPESEFELLRSNRKEKHLQRLAKNDYLANRLFLSNMWGKTHPY
                     GRVTELENFDQMQVADVHDFFYQHYHAGNCFIILAGEYTDATIKQIDELFGAADWKKQ
                     PSTSILHQRSMGQELIIHQEKENAVQTSLMVGNATITKTHPDFDELTIVNTLFGGYFG
                     SRLMANIREEKGYTYGVYSSLSAYQHGAIFEVSADVGKEHKDATFHEIKKEMQRLQTE
                     LVDEEELQTVKNYMSGRILRSVDGALRYADVLKGLLLFDREPDSINSYLNAIQTIDAK
                     RIQTLAEQYLQFDSMYKVSVG"
     gene            1291..>1908
                     /locus_tag="PKJ43_06965"
     CDS             1291..>1908
                     /locus_tag="PKJ43_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72345.1"
                     /translation="MIKRNKYCTSHINNNILKAFAFLLLMSCAVFYTNAQTILAPRLT
                     CIVNDFVGGNVSISWENVNNPCGPFVSYKIYGSNTKAGPYALITTISSQAQTNYIHTN
                     ALSPNGTWYYYMEADFNCPGFTPAQSGTIQNESNPKVPQILSVDVDSTNHVTFNWLPS
                     ESPQTKFYIVYAYLPNGGVVPIDTVYGRFNTSYYDLIEDPTQLSLG"
BASE COUNT          595 a          352 c          403 g          558 t
ORIGIN      
        1 ggaagctcct ccggtgaaaa ttgccgatca catcaacatt ccgcatacag aagaaatggt
       61 gttgagtaat ggtataaagg tgtatggcat tccaaacgat caaggtgtta ttaaggttga
      121 ttttgtgttc gatgccggaa aatggttcga gccaaaaaat ctggtagccg attttgcgaa
      181 caaattaatg cgcgagggca cgaaacaaaa atcatcatta gcgattaatg aaatactcga
      241 tttctatggc tgtaatttag aagagcaatc ctatttttcg aatgccggat ttcagttgta
      301 ttcgctgtcg cgtaatattc atcaagtgtt acctttaatg aaggaaatgc ttacagaggc
      361 tgcttttcct gaatccgagt ttgaattgtt gcgttctaac agaaaagaaa aacacctgca
      421 gcgtttggct aaaaacgact atctcgctaa ccgattattt ttgtcgaata tgtgggggaa
      481 aacacatccc tacggaagag taaccgagct tgaaaacttt gatcaaatgc aggtggccga
      541 tgtgcacgac tttttctatc aacattatca tgctgggaat tgctttatca ttttagcagg
      601 agaatatacc gatgcgacca ttaaacaaat cgatgaactt ttcggtgctg cagattggaa
      661 gaaacaacct tcaacgtcta ttcttcatca aagaagtatg ggacaagagc ttatcatcca
      721 tcaagaaaag gaaaatgcag tccaaacttc attaatggtg ggcaatgcaa ccatcactaa
      781 aacgcatccc gattttgacg agttgaccat agtgaatacc ttgtttgggg ggtatttcgg
      841 ttctcgtttg atggccaata ttcgcgaaga aaaaggatat acctatggcg tttattcttc
      901 tctaagtgct taccaacatg gtgccatttt tgaagtaagt gcagatgtag gaaaggaaca
      961 taaggatgca acattccatg aaataaaaaa ggaaatgcag cgtttacaaa ccgaattagt
     1021 agacgaagag gaattgcaaa cggtgaaaaa ttatatgagc ggacgaattt tacgcagtgt
     1081 ggatggggct ttgcgctatg cggatgtgtt aaaagggttg ctcttattcg atcgtgagcc
     1141 ggatagtatt aacagttact tgaacgccat tcaaaccatt gatgcaaaac ggattcaaac
     1201 attagcggaa caatacctgc agtttgattc gatgtataaa gtttcagttg gataattggc
     1261 gttattattg gaaagaatag ttttgtaaga atgattaaac gaaataaata ttgtacctca
     1321 cacataaaca acaacatctt aaaagcattt gcttttttgt tgctgatgtc ttgtgctgtt
     1381 ttttatacga atgcgcaaac cattttagcc cctcgattaa cctgcatagt caacgatttt
     1441 gttggaggta atgtgtctat ttcatgggaa aatgtcaata acccttgtgg tcctttcgta
     1501 tcgtataaaa tttatgggtc aaatacgaaa gccggtccct atgcattgat tactaccata
     1561 tcatctcagg cacaaacaaa ctatatacat acgaacgccc tttcgcctaa tggaacttgg
     1621 tattattata tggaggccga ttttaattgt ccgggtttta cacctgctca gtccggtact
     1681 attcaaaacg aatcgaatcc gaaagtgccg cagatattga gtgtggatgt tgactcaacg
     1741 aaccatgtaa ctttcaactg gttgccaagt gaatcgcccc aaaccaaatt ttatatcgtt
     1801 tacgcatatc tgccgaacgg gggagtagtg cctatcgata cagtttacgg gcgtttcaat
     1861 accagttact acgatttaat tgaagatcca acacaacttt cattaggc
//
LOCUS       DAOPUW010000396         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_65829_length_1907_cov_3.631210, whole genome shotgun sequence.
ACCESSION   DAOPUW010000396 DAOPUW010000000
VERSION     DAOPUW010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65829_length_1907_cov_3.631210"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PKJ43_06970"
     CDS             complement(<1..235)
                     /locus_tag="PKJ43_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72346.1"
                     /translation="MKFAFNFDATDHIFYKYYTGMVSFSDFVSSWQYIISNKFLPSDA
                     KGILLDYRKAIILSPIDEALKVSDYFSVNGTVFR"
     gene            622..>1907
                     /locus_tag="PKJ43_06975"
     CDS             622..>1907
                     /locus_tag="PKJ43_06975"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF017493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNZ72347.1"
                     /translation="MAEFVYQKPYPIQKDSTNYRLLTKEYVSTVEFEGRKILKVSPEG
                     LSLLAREAMADVSFYLRASHLEKVRSILSDPEATDNDKFVAHTMLVNQVVAAEGDLPT
                     CQDTGTAIVIGKKGENVFTGVNDAEYLSKGIFETYRDRNLRYSQVVPFTMLEEKNTGT
                     NLPAQIDIYAEEGNKYEFLFVTKGGGSGNKTFLYQQTKALLTEEALTKFVKEKILDLG
                     TSACPPYHLALVIGGTSAEATLAAVKKASAGYFDHLPTHGNEGGQAFRDLEWEEKVLK
                     ICRESGIGAQFGGKYFVHDVRVIRLPRHAASCPVGLGVSCSADRNVKGKITEEGIFLE
                     VLEKNPRRFLPEKAPELAAPVEIDLDRPMEEILAELSKYPVKTRLNLKGTLIVARDIA
                     HAEIKKIIDSGKPMPEYFKKHPVYYAGPAKTPKGMAS"
BASE COUNT          624 a          409 c          387 g          487 t
ORIGIN      
        1 gccgaaagac agtcccattg accgaaaaat aatcggatac ctttaatgct tcatcgattg
       61 gcgaaagtat aatcgctttc cggtaatcga gcaatatccc ttttgcatct gagggtaaaa
      121 atttattact gattatgtat tgccacgaag aaacgaaatc actaaaagaa accatacctg
      181 tgtagtattt ataaaatatg tgatccgttg catcaaaatt aaaagcgaat ttcatcccca
      241 ttatttttta tgatggcaca aaattaaaac ggaaaataat tataaaacag gtatttccaa
      301 taagtttgac caacgtcggg aaacgtatgg caaaagcacg ttttatcctg attggttgat
      361 ttattttgtt aaaaacaatg acagtattgc aatagagaac agcaattacc catcaaatat
      421 cacttccttt ctcagcccgt tttatctggt atcgattagc ccaagaggtc tggagtatcc
      481 gttgaagccc cagatggaaa ataacaatgc tatcttttcc tgaaaacgat aatctaatca
      541 catcacccga tatgcatttc aaaaggccat tcgttatatt tgcaaggtga aaaattaaaa
      601 aaatcagaaa aacataaaat catggcagaa ttcgtatatc aaaaaccgta tcccattcaa
      661 aaagacagca ccaattatcg tctactcacc aaagaatatg tatcgaccgt tgaatttgaa
      721 ggaagaaaaa tcctgaaagt ttcgcctgaa gggctttctt tgcttgcccg cgaagctatg
      781 gccgatgtat ctttttacct acgtgcatcg cacctcgaaa aagtccgttc cattcttagc
      841 gatcctgaag ccacagacaa cgacaaattt gtagcccaca ccatgctggt taaccaggtg
      901 gttgctgccg aaggtgattt gccaacctgt caggataccg gtactgccat tgtgattggg
      961 aaaaaaggag aaaatgtatt caccggggtt aacgatgccg agtatctttc aaagggaata
     1021 tttgaaacat atcgcgaccg taacctaaga tactctcagg tggtgccttt caccatgctc
     1081 gaagagaaaa acacagggac aaacctgcca gctcagattg atatttatgc cgaagaaggc
     1141 aacaaatatg aattcttatt cgtcactaaa gggggtggtt cagggaataa aacctttctt
     1201 tatcaacaga ccaaagcact cttgaccgaa gaggcactga caaaatttgt caaagaaaaa
     1261 attcttgatc taggcacatc ggcttgccct ccttaccatt tagccctagt aattggagga
     1321 acttcggctg aagcaactct ggcagccgta aaaaaagcat cggccggata ttttgaccac
     1381 ttgccaacac acggaaacga aggcggacaa gccttccgcg acttggaatg ggaagaaaaa
     1441 gtattgaaga tttgccggga aagtggcatt ggtgcccaat ttggcggaaa atatttcgtt
     1501 cacgacgttc gcgtaatccg tttgccacgc cacgcagctt cttgtcctgt gggattagga
     1561 gttagctgta gcgccgaccg caatgtaaaa ggaaaaatta ccgaagaggg catattcctt
     1621 gaagtgttgg aaaaaaatcc acgccgtttc ttgccggaaa aagcacctga attagctgcc
     1681 ccggttgaaa tagatctcga ccgcccaatg gaagaaattt tggccgagtt aagcaaatac
     1741 ccggtaaaaa cacgtttaaa cctgaaaggc accctgattg ttgcccgtga cattgcccac
     1801 gctgagatta aaaaaatcat cgattcggga aaaccaatgc cagaatactt taagaagcat
     1861 cctgtttatt atgccggccc tgctaaaaca cctaaaggaa tggcttc
//
LOCUS       DAOPUW010000397         1904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_66020_length_1904_cov_2.799892, whole genome shotgun sequence.
ACCESSION   DAOPUW010000397 DAOPUW010000000
VERSION     DAOPUW010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66020_length_1904_cov_2.799892"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="PKJ43_06980"
     CDS             complement(<1..946)
                     /locus_tag="PKJ43_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNZ72348.1"
                     /translation="MKRKFIYIGIAVVLGIVALVALKGNSASKATFAIETGKIERGNI
                     SSLVTATGTLEAITTVEVGTQVSGVIEKIYVDFNTQVKKGQLLAQLDKTPLQAQVDQS
                     QATVDNAEAELVYQKSNFERTKALFDKQLIAKTDYDLALYNYNKAQASLKNAKSVHDK
                     NKINLSYASIYSPIDGIILDRAVDEGQTVAASFNTPTLFTIANDLTQMQVEANVDEAD
                     IGQVVEGQRVEFTVDAYPDMKFAGAVSQLRLQPVETNNAITYTVIVNAPNPDLKLMPG
                     MTANISAFTKEKNGIWVVPGKAIRFNPDANLIAEYLTSI"
     gene            complement(1099..1761)
                     /locus_tag="PKJ43_06985"
                     /pseudo
     CDS             complement(1099..1761)
                     /locus_tag="PKJ43_06985"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
BASE COUNT          501 a          412 c          392 g          599 t
ORIGIN      
        1 caatggacgt taggtactcg gctatcaaat tagcatcggg attaaagcgg atggctttcc
       61 caggaacaac ccaaatacca tttttctctt tggtgaaggc cgaaatgttg gcagtcatgc
      121 cgggcatcaa cttcaaatcg ggattgggtg catttacaat taccgtgtac gtaatagcat
      181 tatttgtttc aacgggttgc aaacgcaact gtgaaacagc ccctgcaaat ttcatgtcag
      241 gatacgcatc aacagtaaac tcaacacgtt ggccttctac tacttgacct atatcggctt
      301 catcaacatt tgcctcaact tgcatctgag tcaggtcgtt cgcaatggtg aacaacgtgg
      361 gcgtattaaa gctggctgca actgtttggc cttcgtcaac cgcacggtcg agaataatcc
      421 catctatggg cgaatagatt gaagcataag acaagttgat cttgttttta tcgtgaaccg
      481 atttcgcgtt tttcaaactc gcttgtgctt tgttgtaatt gtaaagcgct aaatcgtaat
      541 cggttttagc gatcaattgc ttatcgaata gagccttagt gcgttcgaag ttcgattttt
      601 gataaaccag ttcggcttcg gcattgtcga cagttgcctg actttgatct acttgcgctt
      661 gaagcggcgt tttatcgagc tgagctaaaa gttgtccttt ttttacctgg gtgttgaaat
      721 cgacatagat cttttcgatc acacccgaca cttgcgtacc aacctcaacg gtagtaatgg
      781 cctcgagcgt tccggttgcc gttaccaaac tgctgatgtt tccgcgttca atttttccag
      841 tttcaatggc aaacgttgct ttcgatgcgc tgttgccttt cagtgctacc agagctacta
      901 tgcccaaaac cactgcaatt cctatgtaaa taaattttcg tttcatgatc ttataaatta
      961 cccctaaatc ccttcctcga tagctatcgg gagtggactt ttacgggagt gtgaaattgt
     1021 tattttatct ctttaattta ttgtattaaa agaacttact ttcaaatttt gtagttactc
     1081 aagtcccctt taggggattt aggggttatt gtaaaaatca actattttat tactgaaaac
     1141 caagttgaat ttcgactgaa gtaactgact ttcagcgctt atcaatttgg tcttttcaaa
     1201 aagaaaatcg accgaattca atgcacccaa cttgaacttt tcttcagcca ccttatacga
     1261 ttccgatgtg gctttcaact gatcgatact agcctcatac tgtgtttgag ccgacacggc
     1321 atcggtatat gcttgttcaa tttctttgcg caattggttg cgggtgttca gttcacttag
     1381 cgtcgcattg tcgactccaa tcttggccaa agccacgttg ttttacactt ggtttttctg
     1441 aaaaattggg atcgaaagag tgagcccaac actaggacta atgctgttgt tcaactgatt
     1501 ggcatagttg aaattggaat tcaaacttga ataacccgtt ccaatgcccg cgtccatcga
     1561 caacgaaggt aagcccgcag cttgagcaat tttggcatca atttcagcac tttgaatatt
     1621 caacttcgct tcaccaattt gcggtttcaa ctttaaggca gtttcataaa ttgcagcagc
     1681 agtgagcgat gcgtcttgtt gaatcagcgc atcgatgttt ggcgaagcaa ctgtgaaagc
     1741 ctctgataca ggaagttcca tcaattgctg caaattcact ttactcattg aaagctgctt
     1801 ttgagcattg acaagcgtta atttttcgct ggctaattgc gattcaatca gcaaaagatc
     1861 ggaacgagaa atagaattca actcgaagcg tgctttggcc aaag
//
LOCUS       DAOPUW010000398         1902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_66117_length_1902_cov_3.022740, whole genome shotgun sequence.
ACCESSION   DAOPUW010000398 DAOPUW010000000
VERSION     DAOPUW010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66117_length_1902_cov_3.022740"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PKJ43_06990"
     CDS             complement(<1..503)
                     /locus_tag="PKJ43_06990"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNZ72349.1"
                     /translation="MAEKIIEPIEEYGLSKEKKSGKLFSKVGIVGCGNDGQYIARVCS
                     SYGMDVLFWEATPEKIEKSLNSISDIMESRIRNWGATVSEKKAVMSRIKATTSLKDMK
                     DCDLVVETASLTSIGRSVDSRRKVFEEIEKIVSPTCIIASNSTTVMISELTENLKYKD
                     RCISIHF"
     gene            complement(513..1511)
                     /gene="pta"
                     /locus_tag="PKJ43_06995"
     CDS             complement(513..1511)
                     /gene="pta"
                     /locus_tag="PKJ43_06995"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HNZ72350.1"
                     /translation="MDLLKQIKQNAAKYNMRIVLPEGTEPRTLKAAQIIIKEKLARLI
                     LLGNKAEVEKKSAELNVEISGAEIIDPTTDLLREKYAEKMVEIRKEKGLSHEDALKML
                     NDPLCFGAMMIKCGDADGELAGAINSTGDVLRPAFQFVKTAPGSSVVSGAFFMFVDQK
                     FGADGILVFADCAVLPDPSAEQLAEIAVTTAKTARAITGMEPRVALLSFSSKGSAKHP
                     LVDKVRKATELAQQMAPELMIDGELQLDAALVKEVGELKAPGSKVAGKANVLVFPGLE
                     SGNIGYKLVQRLAGAEAVGPVLQGMAAPINDLSRGCSVSDIVNMVAITANQAANKF"
     gene            1660..>1902
                     /locus_tag="PKJ43_07000"
     CDS             1660..>1902
                     /locus_tag="PKJ43_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72351.1"
                     /translation="MRFLKIVKFGSTVSKSVIFEKIYTIIMRKSITILLLFLVQLAFS
                     QINTNNVIHTGRSRLYFGNYVGAIESFNMVIKMKPYL"
BASE COUNT          521 a          412 c          331 g          638 t
ORIGIN      
        1 atgaagtgaa tgcttatgca acggtcttta tatttcagat tttcagttag ttctgaaatc
       61 atcactgtgg tggagtttga cgcaatgatg caggtcggcg aaactatctt ttcaatttct
      121 tcgaaaactt tacgtctaga atctacgctg cggccaatgg aggttagtga tgcagtttca
      181 acaactaaat cgcaatcttt catgtcttta agagaagttg ttgcctttat tctcgacata
      241 acagccttct tttcagaaac ggtagcaccc caatttctga ttcgactttc cataatatcg
      301 gaaattgaat ttagtgattt ttctattttt tcaggagtag cctcccagaa cagtacatcc
      361 attccatatg agctgcaaac tctggcaata tattgcccat catttccaca acctacgata
      421 ccaactttgg aaaagagttt tcccgatttc ttttccttgc ttagcccata ctcctcaatg
      481 ggttcaatta tcttttctgc cattttagtt ttttagaatt tatttgcggc ttgatttgca
      541 gtgattgcca ccatattaac aatatcagag accgaacatc cacgtgacaa atcgttgatt
      601 ggggcagcca ttccctgtaa tacaggccct acggcttctg ctcctgcaag gcgttgcacc
      661 aatttatatc caatattacc cgattcaagt cctgggaata ccagtacatt ggcttttcct
      721 gccactttgc tgcctggagc tttcaactcg ccaacttctt taaccagtgc tgcatcgagt
      781 tggagttcgc catcgatcat cagttcagga gccatctgct gagctaattc tgtcgctttt
      841 cttactttgt cgactaacgg atgttttgca cttcctttgc ttgaaaagct taaaagggct
      901 actcgtggtt ccatgcctgt aatagctctg gctgttttag ctgtggtaac agcaatttca
      961 gcaagctgtt ctgcactagg gtcgggcaat acagcacaat cggcaaaaac aaggatgcca
     1021 tcagctccaa atttttgatc aacaaacata aagaatgctc cagagacaac actgctccct
     1081 ggggctgttt tcacaaactg aaaagcaggt cggagaacat cgcctgttga attaattgca
     1141 ccggcaagct caccatcagc atcgccacat ttgatcatca ttgccccaaa gcaaagtgga
     1201 tcgttaagca ttttcaacgc atcttcgtgg cttaatccct tttctttcct gatctcaacc
     1261 attttctctg cgtatttttc ccttagcaaa tcggttgttg ggtcaataat ttcagccccg
     1321 gatatttcaa cattaagttc tgccgatttt ttctcaactt cagccttgtt tcccaataaa
     1381 attagtctgg ctagtttctc tttgataata atttgcgcag ccttcaacgt tcttggttct
     1441 gttccttcag gaagtacaat tctcatattg tacttggctg cattttgttt aatttgtttc
     1501 agtaaatcca tcacttactc tatttctggt aaaaaaaaga agtacaaaag taaccttttt
     1561 gtccgcttgt tataattgaa aaaaatcatt tcgacgatat ttttcaaaat ttttaatttt
     1621 cacagaagtt agctattcgc aagggaaaaa tcaatattca tgcgattctt aaaaatcgtg
     1681 aaattcggat caacggtctc taaaagtgtt atttttgaga agatttacac gataattatg
     1741 agaaaatcca tcacaatact tttgcttttt ttagtacaat tagcatttag tcagataaat
     1801 accaataatg ttattcacac cggacgtagc cgcttatact ttggaaacta tgttggagca
     1861 attgaaagtt tcaacatggt tattaaaatg aagccttatt ta
//
LOCUS       DAOPUW010000399         1902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_66118_length_1902_cov_2.952355, whole genome shotgun sequence.
ACCESSION   DAOPUW010000399 DAOPUW010000000
VERSION     DAOPUW010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66118_length_1902_cov_2.952355"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PKJ43_07005"
     CDS             complement(<1..280)
                     /locus_tag="PKJ43_07005"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNZ72352.1"
                     /translation="MKTHFFLLLLIFTSLQSFAQITIKGNVTDENKEPLPGVNIYVEG
                     SYDGGSSDANGCFNFTTTETDTKKIMASFIGFKTWEKEIDLSTSVAIDI"
     gene            complement(530..1597)
                     /locus_tag="PKJ43_07010"
     CDS             complement(530..1597)
                     /locus_tag="PKJ43_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNZ72353.1"
                     /translation="MNKKSKIYVAGHKGLVGSAIWKTLLNKGYSNLIGQTIEELDLMD
                     SHAVSRFFNQEKPEYVFLAAAKVGGIVANNTYRGQFIYENLMIQNNVIHQAYQAGVKK
                     LLFLGSTCIYPCEAPQPMPEDCLLTSPLEYTNEPYAIAKIAGIKMCESYNLQYGTNFL
                     SVMPTNLYGPNDNFNLETSHVLPALIRKIHLGRCLEDNNWQAIRSDLNKRPIEGVNGN
                     SDEKTIIEKLSNYGIIAGGVEIWGSGAPMREFLWSEEMAEACVFVMENVNFDDVRDNQ
                     KEVRNTHINIGTGKEISIKNLAGLIKEIVGFKGELRFNTSKPDGTLRKLTDPSKIHKL
                     GWHHKIEINDGIQLLYKWYLG"
     gene            complement(1660..>1902)
                     /locus_tag="PKJ43_07015"
     CDS             complement(1660..>1902)
                     /locus_tag="PKJ43_07015"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72354.1"
                     /translation="ILEKDIHRFAEAFLDSFRSQTQMFPAMINPQVQKAIDEYKDGAL
                     AWKLAGAGGGGYLILVSEEPVKGAMRIKVRRKDSGI"
BASE COUNT          515 a          364 c          398 g          625 t
ORIGIN      
        1 taatgtcgat tgcaaccgat gttgaaaggt caatctcttt ttcccaggtt ttaaaaccga
       61 tgaaactggc cataattttt tttgtgtctg tttcggtggt tgtgaaatta aaacacccgt
      121 ttgcatcgga actacctcca tcgtatgagc cttcgacata gatatttact ccgggaagag
      181 gttctttgtt ttcgtcagtt acatttcctt tgatcgtaat ttgagcaaaa gattgcagtg
      241 aagtgaaaat aagaagcaat aaaaagaaat gtgttttcat ggctaaatcg tatcaagatt
      301 aaacgaacgc cacaaaattg gatactgtaa aacgggcagg gaaaaattcc ttactgaaat
      361 gtaggaaatg taggatggag tggggaagtg aagtgagggt gaaaatgaaa atggcgtgaa
      421 ttgtggaatt gaattccata attcacgcca aattgtggaa ttgatctcca cgattagctt
      481 ccttttttaa actttaccga aaataaagta gctgtctttg aataaaaggt tagcctaagt
      541 accatttgta tagtagttga attccatcgt tgatttcgat tttatggtgc caacccaatt
      601 tgtggatttt tgaaggatcg gtgagttttc gcaacgtccc atcgggtttg gacgtattga
      661 agcgtaattc tcctttgaat ccaacaattt ctttgataag accggcaagg tttttaatgc
      721 tgatttcttt gccggttcct atattaatat gtgtatttct cacttccttt tgattgtctc
      781 tgacatcgtc aaaatttaca ttctccatta caaagacaca agcttcggcc atctcttcac
      841 tccagagaaa ttcacgcatg ggagcacctg atccccaaat ttcgacacct cctgcgatta
      901 ttccgtagtt ggacagtttc tcaattatgg ttttttcatc tgaattgcca ttaacccctt
      961 cgattggtcg tttgttcagg tcggaacgta ttgcctgcca gttgttatct tccaggcatc
     1021 ttccgagatg tatcttgcgg attaatgctg gcaacacgtg agatgtttcg aggttgaaat
     1081 tgtcatttgg tccgtagagg ttagttggca taacgctcag gaaatttgtc ccgtattgca
     1141 ggttgtagct ttcgcacatt ttgattccgg caattttggc aatggcatac ggctcattgg
     1201 tatattcaag cggcgaagtg agcagacaat cttcaggcat gggttgcggg gcttcacacg
     1261 ggtaaatgca agtgcttccc agaaacagaa gctttttaac gccagcctga taggcctgat
     1321 ggattacatt gttttggatc atcaggtttt cgtaaatgaa ctgaccgcga taggtgttat
     1381 tggcaacaat tccacccact ttagcagcgg caaggaatac atattctggc ttttcctggt
     1441 taaaaaagcg tgatactgca tggctgtcca tcaaatctaa ctcctcaatg gtctgaccaa
     1501 tcaaattgga ataaccttta tttagtagag ttttccaaat tgcagaacca acgagtcctt
     1561 tgtgtccggc aacatatatt ttgctttttt tattcatact ttacgttttt cgattttata
     1621 taaaagtaat gagatgtgat tgttgaattg gtatttgtct taaattcccg agtcttttct
     1681 ccgaacttta attcgcattg ctcctttaac tggttcttcc gaaacgagga ttaaataacc
     1741 acctccaccg gcaccagcaa gtttccatgc taatgcgcca tctttatatt catcgatagc
     1801 cttttggacc tgagggttaa tcattgcagg aaacatttga gtttgcgagc ggaatgaatc
     1861 gagaaatgct tcagcaaatc gatggatgtc tttttcaaga at
//
LOCUS       DAOPUW010000400         1901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_66160_length_1901_cov_4.327736, whole genome shotgun sequence.
ACCESSION   DAOPUW010000400 DAOPUW010000000
VERSION     DAOPUW010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66160_length_1901_cov_4.327736"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..711
                     /gene="cysC"
                     /locus_tag="PKJ43_07020"
     CDS             118..711
                     /gene="cysC"
                     /locus_tag="PKJ43_07020"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNZ72355.1"
                     /translation="MNNIFPVFEQIVPREQKENKLKQKSVVIWFTGLSGAGKTTLAVA
                     LEKELFQRGFLTQILDGDNIRSGINNNLKFSPEDRTENIRRIAEVSKLFMNCGIITLC
                     AFVSPTQDIRQLVRNIVGTENFYEIFVSTPVEVCEQRDVKGLYAKARSGEIKDFTGIN
                     APFEIPTDANFVIDTSLQSVETNVEILLKELLPIITK"
     gene            723..1634
                     /gene="cysD"
                     /locus_tag="PKJ43_07025"
     CDS             723..1634
                     /gene="cysD"
                     /locus_tag="PKJ43_07025"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNZ72356.1"
                     /translation="MINEHNLSHLKELEAEAIHIIREVAAEFENPVMLYSIGKDSSVM
                     ARLAEKAFFPGKVPFPLMHIDSKWKFREMIEFRDNYTKEKNWDLIVHYNKEAYEAGVG
                     PFTHGSKVHTDIMKTQALLDGLNKYKFDAAFGGARRDEEKSRAKERIFSFRDRFHQWD
                     PKNQRPELWDIYNTKIQKGESIRVFPISNWTELDVWQYIRLEKIPIVPLYFAKERPIV
                     NINGAMILADDDRMPKELRDKAEMKMVRFRTLGCYPLTGAIESEASTIEEIVEEMMTV
                     TVSERTTRVIDFDQEASMEQKKREGYF"
     gene            1703..>1901
                     /locus_tag="PKJ43_07030"
     CDS             1703..>1901
                     /locus_tag="PKJ43_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72357.1"
                     /translation="MINRSNTLDIQTFLAQDEQKDLLRILTAGSVDDGKSTLIGRLMF
                     DSKMIYEDQLAALERDSKRVGH"
BASE COUNT          645 a          404 c          399 g          453 t
ORIGIN      
        1 gagcattaca caacccgatg gcacagcagt tagctacaac aaagaaaacc tgctgagccc
       61 ttttttcatt gtgaaataaa aaaataacca ataggatatc aacaaattaa ccaactaatg
      121 aataatattt ttcctgtctt cgagcaaatt gtaccccgtg aacaaaaaga aaacaaactc
      181 aaacagaaat cggtagttat ctggtttact ggcttatcgg gagcgggcaa aaccacactg
      241 gctgttgccc tcgaaaaaga actttttcaa cgcggatttc tcacccagat actcgatggc
      301 gacaatatcc gctcgggaat caataacaac ctaaaattca gccccgaaga ccgaaccgaa
      361 aatatacggc gcattgccga agtctctaaa ttattcatga actgcggcat aataaccctt
      421 tgtgcttttg taagccccac gcaggatata aggcaattag ttcgcaacat tgtaggaact
      481 gagaactttt acgaaatatt tgtaagcact cctgttgaag tgtgcgaaca gcgcgatgtt
      541 aaagggcttt atgccaaggc ccgctcaggg gaaataaaag attttactgg aatcaatgcc
      601 ccattcgaaa ttccgaccga tgccaatttt gtgatcgaca cttcactcca atctgtcgaa
      661 acgaacgtag aaattctgct gaaagagcta ttgcctatta tcacgaaata attgattcaa
      721 ctatgataaa cgaacacaac ctgagccact taaaggaact tgaagccgaa gcaattcaca
      781 taatccgcga agtggctgcc gaattcgaaa acccggtaat gctttattca ataggaaaag
      841 attcatcggt tatggcacga ttagcagaga aggctttttt cccggggaag gtacctttcc
      901 cgttaatgca catcgattcg aaatggaaat tcagggaaat gatcgaattt cgcgacaact
      961 ataccaaaga aaagaactgg gaccttattg tgcattacaa taaagaagct tacgaagccg
     1021 gtgttgggcc attcacccat ggcagcaaag tgcataccga tataatgaaa acccaagcac
     1081 tgctcgacgg gctaaataaa tataagttcg atgcggcatt tgggggtgcc cggcgcgacg
     1141 aagaaaaatc gagggccaaa gaacgcattt tctcattccg tgaccgtttt caccagtggg
     1201 accctaaaaa ccagcggcct gaactttggg acatatacaa caccaaaatt caaaaagggg
     1261 aatcgatccg ggtttttcca atctcgaact ggacagagtt agatgtgtgg caatatattc
     1321 gactcgaaaa aataccaatt gttcccctat actttgccaa agaacgtccc atcgtaaaca
     1381 taaacggtgc catgatttta gccgatgatg accgcatgcc aaaagagctt cgtgataaag
     1441 ccgagatgaa aatggttaga tttagaacat taggatgcta tccgttaacc ggagctattg
     1501 agtcagaagc gagcaccatt gaagaaatcg ttgaagaaat gatgacagta accgtctctg
     1561 aacgaacaac aagagtgatc gatttcgatc aggaagcgag catggaacaa aagaaaagag
     1621 aagggtattt ttaaaattca gtttatagca aaaacttaat ttttgaatac ttggaaatcg
     1681 actatatctt aaaacaatta ctatgatcaa tagaagcaat acattagata tacaaacctt
     1741 ccttgcccag gatgagcaga aagacctgct tcgaattctc actgcaggat cggtcgacga
     1801 tgggaaatca acgctcatcg ggcggcttat gttcgacagt aaaatgattt acgaagatca
     1861 gttagctgcc ctcgaacggg acagtaaacg agttgggcac g
//
LOCUS       DAOPUW010000401         1901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_66186_length_1901_cov_2.785482, whole genome shotgun sequence.
ACCESSION   DAOPUW010000401 DAOPUW010000000
VERSION     DAOPUW010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66186_length_1901_cov_2.785482"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /gene="rlmN"
                     /locus_tag="PKJ43_07035"
     CDS             complement(<1..674)
                     /gene="rlmN"
                     /locus_tag="PKJ43_07035"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNZ72358.1"
                     /translation="MKQDIRELSQAEMEQAFAALGVEKFRAKQVHEWLWKKSAQSFEQ
                     MTNLSKSFREELTNHFSFDLLQESIAQKSSDGTVKLGIRLKDSRMVEGVMIPDGERAT
                     ACVSSQVGCTLSCAFCATGFLKRERNLTAAEIYDQVVLLNQHAKDHNGKGLTNIVYMG
                     MGEPLLNYDNVLQSIYLITSPEGLNMSQKRITVSTSGISRGIVRLADENKKFNLALSL
                     HAATNE"
     gene            922..>1901
                     /locus_tag="PKJ43_07040"
     CDS             922..>1901
                     /locus_tag="PKJ43_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNZ72359.1"
                     /translation="MFKESATLDFHYNKIDEAALAFFRSIVGDEFVLTTGEHYEKYAK
                     DETENLRFYPEAVAKPNTTAQVSALVRYCNEHKIPVYPRAAGTGLSANSLAIYGGLMI
                     TIERLNKIIEIDENNLQVTTEPGVITELLQNTVKEKGLFYPPDPSSRGTSFIGGNISC
                     NSGGPRAVKYGVVKDYVLNLEVVLPNGEVMWTGANVLKNSTGYNFTHLFVGSEGTLGI
                     VTKIVLKLIPHPKFNVIMLVPFTSMDQASAAVSAIFRAGITPSCLEFMERDAIDWVCN
                     WLGDVTIPIADDVRANLLIELDGNDMDYIQKEAEHLYEVLTQFDCGEILFA"
BASE COUNT          562 a          440 c          350 g          549 t
ORIGIN      
        1 ttttcatttg tagccgcatg taacgaaagt gctaaattga actttttatt ttcatcggcc
       61 aatcgcacaa tccctctgct aataccactt gtagacacag taattcgctt ttgcgacata
      121 ttcaatccct ccggagaagt aatcaaataa atactctgca gcacattatc gtaattcaac
      181 aacggctcgc ccatacccat ataaacaata ttagtcagcc ccttaccatt atggtcttta
      241 gcgtgttggt tcaataaaac cacttgatca taaatttcag ctgccgttag atttcgttca
      301 cgctttaaaa aaccggtagc gcaaaaagca caacttaagg tacaacccac ttgtgaggaa
      361 acacaagccg ttgcccgctc cccatccgga atcattaccc cttccaccat tcgactgtct
      421 ttcaagcgaa tgcctaactt tactgttcca tcagaactct tttgggctat gctctcttgc
      481 aataaatcga atgaaaagtg attcgttagt tcttcgcgaa acgatttcga caaattcgtc
      541 atttgctcaa acgattgagc cgactttttc cagagccact catgcacttg ctttgcacgg
      601 aatttctcaa ccccaagtgc agcaaaagcc tgctccattt cagcctgcga taattcccgt
      661 atatcctgct tcatagcgcg aaaatagcaa gaaaaaggaa ctggtgatgc ccaattctca
      721 aatttcaatt ttcagttatt cattcaaaaa ttctcaattc gctcatcctc ccattatcaa
      781 attacctaat cctccattgt taaatcattt cactgttaaa ttcagcattt ttcgccatca
      841 tcaaatttac acattataaa atcaccaaat taatcccctt cccacgttgc ctacttctaa
      901 ttaaaaacta attttacgac tatgtttaaa gaatcagcaa cactcgattt ccactataat
      961 aaaatagatg aagccgcttt ggcatttttc cgttccattg tgggtgatga atttgtttta
     1021 accacgggag agcattacga aaaatacgcc aaagacgaaa cagaaaacct ccgcttctac
     1081 cctgaagctg tagccaaacc aaataccaca gctcaagtaa gtgccctggt gcgctattgc
     1141 aatgaacata aaattccggt ttacccacgt gcagccggaa caggtctttc ggctaacagc
     1201 ttggcgattt atggcggctt aatgataacc atcgaacggt taaacaagat tattgaaatc
     1261 gatgaaaaca atttgcaagt taccaccgaa ccgggcgtta ttactgaatt gttgcagaat
     1321 accgttaaag aaaaaggctt gttctatcct cccgatccgt caagccgagg aactagtttt
     1381 attggtggaa atatttcgtg caattcgggc ggaccaagag ccgtgaaata cggggtagta
     1441 aaggattatg tacttaacct ggaagtggtt ttaccgaatg gtgaagtaat gtggaccgga
     1501 gccaatgtac ttaaaaactc taccggctat aatttcacgc atttgtttgt gggaagcgaa
     1561 ggcaccttgg ggatcgtaac caaaattgtt ttgaaactta tcccgcatcc aaaattcaat
     1621 gtaattatgt tggtgccatt cacctccatg gaccaagcga gtgcagctgt gtcggccatt
     1681 ttccgagcag ggattacgcc ttcttgcctt gaatttatgg agcgagatgc tattgattgg
     1741 gtttgcaatt ggttaggtga cgttaccatt cctattgccg atgatgtgcg cgccaacttg
     1801 ctgatcgagc tcgatggcaa cgatatggat tacatccaaa aagaagcaga acatttgtat
     1861 gaagtactca cacaattcga ttgtggagag attttatttg c
//
LOCUS       DAOPUW010000402         1899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_66296_length_1899_cov_4.005423, whole genome shotgun sequence.
ACCESSION   DAOPUW010000402 DAOPUW010000000
VERSION     DAOPUW010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66296_length_1899_cov_4.005423"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="PKJ43_07045"
     CDS             <1..893
                     /locus_tag="PKJ43_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72360.1"
                     /translation="AKPLWCIVRKARHNILMNRNSKIMMDGKVLEINAVKQQVQQLLD
                     QSLSGIDTQNLDETKSLLKFILVILKDMATDESEYNKVLNIVGNSFSVARDKLANEKF
                     NSGFESLNNENRKMIETIIPLNVFLLKPKQMSKKSETVPPPPPSPLVIKTDGVYLGDK
                     LTLDELKKLIEFNQSDGSIMRFIVRIEEGVTQNKIEEIKQVLKKSEIVRIENSSKQMG
                     EVPPPITIEIKETGIYCMNDLVSLERLKEIITKNIAMHPNQVVSLKVAPEVKMGEVTA
                     VKEVLRKAGALHVNNSTDVK"
     gene            complement(1100..>1899)
                     /locus_tag="PKJ43_07050"
     CDS             complement(1100..>1899)
                     /locus_tag="PKJ43_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNZ72361.1"
                     /translation="KDKPIIKVNCSALPDSLIESELFGHEKGAFTGADSQRKGRFEMA
                     DGGTIFLDEIGDLPSQTQVKLLRVIQERQFERLGSSKPISVDVRIITATNRNLEKLIE
                     EGKFREDLFYRINVFPVFVPPLRERRNDIPGLVDHFIEKFNKENGTKVKRITTAAINM
                     LMVYSWPGNVRELENVVERGCILTTDEVIHSYNLPPTLQTADSSQTETVGGLQPALDR
                     VECQLIMEALTATKGNIAQAAKNLNVTERILGMRVKKYRLDPARFKV"
BASE COUNT          594 a          402 c          381 g          522 t
ORIGIN      
        1 ccgccaaacc gttatggtgc attgtaagaa aagccagaca taatatttta atgaatagga
       61 attctaaaat catgatggat ggaaaagttc ttgaaattaa tgcggttaag caacaagttc
      121 aacaactact agaccagtca ttatcaggaa ttgacacgca aaacctcgat gaaacaaagt
      181 cattattgaa atttattctt gttattctga aagacatggc gactgatgaa tctgaataca
      241 ataaagtgtt aaacattgtt ggcaactcct tttcggttgc acgagacaaa cttgcaaacg
      301 aaaaattcaa tagtggtttt gaaagcctca ataatgaaaa tcgcaaaatg attgaaacca
      361 ttattccttt aaatgttttt ttacttaagc cgaaacaaat gagtaaaaaa agtgagacag
      421 tcccaccacc gccaccatca cccctggtta tcaaaacaga tggggtttat ttaggagaca
      481 agctcacact tgacgaatta aagaaactaa ttgaatttaa ccaatccgat ggttctataa
      541 tgcggtttat cgtaagaatt gaagaagggg taacgcaaaa caaaattgaa gaaataaagc
      601 aagttttaaa aaaatcagaa atagttagaa ttgaaaattc atctaaacaa atgggggaag
      661 ttccacctcc aattactatt gaaattaaag aaacagggat ttattgcatg aatgatctgg
      721 ttagcctcga acggttaaaa gaaattatca ccaagaatat tgcgatgcat cccaaccaag
      781 tagtatcgct aaaagtagcc cctgaagtta aaatgggcga agttactgct gtgaaagaag
      841 tactacgtaa agcaggagca ttacacgtca ataattcaac tgacgtcaaa taatttcaaa
      901 taaaaagctg ctggctttgg tgcgtctcca ttgccagcag ctttaatcat tgtcaaatga
      961 tattgtattg ctacaatgcg tcaaatttaa gtgcaaataa gcaaaacgca aaatcagaac
     1021 accctgcgct tatcaacaac ttttaaatta aaactttagg tacttttaac tctagttcac
     1081 tttaggcact gaataacctc tacaccttaa accgcgcagg gtctaaccta tatttcttca
     1141 ccctcatacc caaaattcgt tcggtaacat tcaggttttt agctgcttgg gcaatgtttc
     1201 cttttgtggc cgtaagggct tccataatca gttgacactc aacacggtcg agtgccggtt
     1261 ggagtcctcc aacagtttcg gtctgcgacg aatcggctgt ttgaagtgtg ggtggcaggt
     1321 tgtagctgtg gatcacctcg tcggtggtga gtatgcagcc gcgttcaacc acgttctcca
     1381 gctcgcgtac gttgccgggc cacgaataca ccatgagcat gttaatggcc gccgtggtta
     1441 tgcgcttcac ttttgtgccg ttttctttgt tgaacttctc gataaaatga tcgaccaaac
     1501 ctggaatgtc gttgcgcctt tcgcgcaaag gtggaacaaa aactggaaac acattgattc
     1561 ggtaaaataa atcttcacga aactttcctt cttcgatcaa cttctccaag ttacggttgg
     1621 tggcagtaat gatgcgcaca tcgaccgaaa tgggtttgct gctacccaag cgttcgaact
     1681 gacgttcctg aatgacacgc aacagcttca cctgtgtttg tgatggcagg tcgccaatct
     1741 catcgagaaa aatggtgccg ccatcggcca tttcgaagcg gcctttccgc tgactgtcgg
     1801 cccccgtgaa agcccctttc tcgtgtccaa acagctcgct ttcgatcaac gaatcgggca
     1861 atgccgaaca gttcactttt ataatcggct tgtcttttc
//
LOCUS       DAOPUW010000403         1899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_66304_length_1899_cov_3.259219, whole genome shotgun sequence.
ACCESSION   DAOPUW010000403 DAOPUW010000000
VERSION     DAOPUW010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66304_length_1899_cov_3.259219"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PKJ43_07055"
     CDS             <1..816
                     /locus_tag="PKJ43_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNZ72362.1"
                     /translation="IDYEKNPSVADDYFQNADTLFCLDFNDFERSEEMKELLAAFGGK
                     RVLIDHHPGPVAQCDVLISCTAVSSTCELLFRVIEDSGLSGYIDKSCADAIFVGMMTD
                     TGNFSYNASDPETYHIIAKLLEIGIDKDLIHSNVYHTFSEDRWRLIGHSLKDKMIILP
                     EYRTGYISLSKRELEQFNYQPGDTEGLVNYPLSVKGIVFCVLFLEKDDYVKMSFRSKG
                     SFSTNEFSRKYFNGGGHINASGGSTKLSMDEAIKFFESLLPQYRNELMDCFPG"
     gene            891..>1899
                     /locus_tag="PKJ43_07060"
     CDS             891..>1899
                     /locus_tag="PKJ43_07060"
                     /inference="COORDINATES: protein motif:HMM:NF016189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HNZ72363.1"
                     /translation="MNRIDLLKRLLPGFIPLFVFIAADEIWGTRVGLIVAVLTGVAEL
                     LYTYIKERRFDKFVLLDTGLLVVLGGVSVAFDNEIFFKVKPAIIEAILCIILAISAFT
                     PVDIIGGMTRRYMKGIEMDEAVLSKFKQTLKAMFFIFTFHTLLIVYAAFFMSKEAWAF
                     ISGGLFYIVFAVYFLYEFWQIRKKKKQLSGMPVVKYEDEEWLPVIDEEGKVIGKALRS
                     ECHSGSKILHPVVHLHVMNPRKLLYLQKRPETKLIQPGKWDTAVGGHIAFGEDVKTAL
                     QREAYEEIGLKDFSAKPIGNYLFESDIEREMVYSFVSYDYKSINLHSDEVTEGKFWSQ
                     KQ"
BASE COUNT          518 a          330 c          440 g          611 t
ORIGIN      
        1 atcgattatg agaagaatcc gtctgttgct gatgattact ttcaaaatgc cgacaccctg
       61 ttttgtctcg attttaatga ctttgagcgc tcagaagaga tgaaggaatt acttgctgct
      121 tttggtggta aacgggtgtt aatcgaccat catccggggc cagttgccca atgtgatgtg
      181 cttatttcat gtacagctgt atcgtctacc tgcgaattgc ttttcagggt tatcgaagat
      241 tcaggacttt cagggtatat cgataaaagt tgtgctgatg ctatttttgt tggtatgatg
      301 accgataccg gaaatttcag ttataacgca tctgacccgg aaacctacca cataattgct
      361 aagctactcg aaattggaat cgacaaagat ttgattcact ctaacgttta tcataccttt
      421 tctgaagaca ggtggcggtt gatcggtcat tcgttgaaag acaaaatgat tattctgcca
      481 gaataccgca ctgggtatat cagcttgagc aaaagagagc ttgagcaatt taattatcag
      541 ccaggggata ccgaaggcct ggtaaattac ccgcttagtg ttaaagggat tgttttttgc
      601 gtgctgttcc tcgaaaaaga tgactatgta aaaatgtcgt tccgttcgaa aggcagtttt
      661 tctaccaatg agttttccag gaaatatttc aatggtgggg gacatatcaa tgcttcaggc
      721 gggagtacca agctttcgat ggatgaagct atcaaattct ttgaaagttt gttgcctcag
      781 tatcgtaacg agttgatgga ttgtttcccg ggttaagtgt tgtgctttta ttttcaattt
      841 aatttgattg cggctttctt tttaccatat tataaatcaa aaaaatcagc atgaatcgga
      901 tcgacttgct taagaggcta ttgcctggct ttatcccctt gtttgttttt attgctgccg
      961 acgaaatttg gggtacgcgt gtaggtttga ttgtagctgt tcttaccggg gtagccgaat
     1021 tactttatac ttatataaaa gagcggcgtt tcgataaatt tgtactgctc gatacgggct
     1081 tgctggttgt attaggtggc gtatctgttg catttgacaa cgagattttt ttcaaagtta
     1141 agccggcgat tattgaggca atcttgtgta taatccttgc aatatccgct tttactccgg
     1201 tcgatataat tgggggaatg acccgtcgtt acatgaaagg tattgaaatg gacgaagccg
     1261 ttttgtcgaa attcaagcaa accttgaagg caatgttttt catttttaca ttccacactc
     1321 ttttaattgt ttatgcagct ttttttatgt caaaagaggc ctgggctttt attagtggcg
     1381 gtttgttcta catcgttttt gccgtttatt ttttatacga attttggcaa atacggaaaa
     1441 agaagaagca gcttagtggc atgccggttg tgaaatacga agatgaagaa tggcttccgg
     1501 ttattgatga agaaggaaaa gtaattggca aagccttgcg ctccgaatgt catagtggct
     1561 ccaaaatatt gcaccctgtg gttcatctgc atgttatgaa tccacgtaag cttttgtatc
     1621 tacagaaaag gcccgagact aaacttattc agccggggaa atgggacact gccgttggcg
     1681 ggcacatcgc ttttggagaa gatgtgaaaa cagcccttca gcgagaagct tatgaggaaa
     1741 tagggctgaa ggatttttcg gcaaaaccaa tcggtaatta tttgtttgaa agcgatatag
     1801 aacgtgaaat ggtctattcg tttgtaagct acgattataa gagtatcaac ttacattccg
     1861 acgaagttac tgaaggaaag ttctggagcc aaaaacaaa
//
LOCUS       DAOPUW010000404         1898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_66353_length_1898_cov_2.923494, whole genome shotgun sequence.
ACCESSION   DAOPUW010000404 DAOPUW010000000
VERSION     DAOPUW010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66353_length_1898_cov_2.923494"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..1720
                     /locus_tag="PKJ43_07065"
     CDS             257..1720
                     /locus_tag="PKJ43_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNZ72364.1"
                     /translation="MGQTNQTTVKFFSGAQIPIELHKVRVVQKLFLKPIEERLLAIEN
                     GGYNTFQLSTKDVFLDMLTDSGTNAMSDNQVSSMLQADDAYAGSQSFYRMEEALREVF
                     GKHYLLPAHQGRAAENIVSQAFIKSGDVIPMNYHFTTTKAHMELNGGTIFEIFTEEAL
                     KIKSSFPFKGNIDTNKLNEIIAKYGKERIPFIRFEASTNLIGGQPFSIQNMRDVKKIA
                     DEHGIMIVLDASLIGENAYFIKKREEEFKNTPISEILKLMCGFADVVYFSSRKVSSTR
                     GGGICTNNRELYMKMRELVILYEGFLTYGGMSVREIEAMAVGLRETTDETVISQSPSF
                     IEYAVNEMNKLGIPVITPAGALGAHIDCMGFLPHVPQEEYPAGALAAALFIISGIRGM
                     ERGTISSVRDANGNDVLSDMELLRLAFPRRVFTLSQTMYVIDRVNWLFHNRDLVGGLK
                     FVEEPAVLRFFNGRLAPTSNWPQKLVAKFKADFGDSL"
BASE COUNT          582 a          402 c          395 g          519 t
ORIGIN      
        1 acccaatact caaaattctg cacgattata aatcaaaaca ttgttgtccg gtaaattaaa
       61 aataacaaac catttgcgaa tgccccgtta tttcggacaa agggagaaat ctcgtaagct
      121 ttaccggaca acagtgaaat caaaaaaata atccaccatg aggattattg taaattattg
      181 ctaaattagt cggcgaaatt tcatttcgta atttattaac aacttttact tacaaattat
      241 aaatcatttc ataataatgg gacaaacaaa ccaaaccact gtaaagtttt ttagtggagc
      301 tcagattcca atcgaacttc ataaagtacg tgttgtacag aaattatttc taaaacctat
      361 cgaagaacgc cttctcgcca tcgagaacgg aggctacaat acgtttcagt tgagtacaaa
      421 agatgttttt cttgatatgc tcaccgactc agggaccaat gccatgagcg ataatcaggt
      481 ttcatcaatg cttcaagccg acgatgcgta tgccggttcg caaagttttt accgcatgga
      541 agaagcactt cgggaagtct tcgggaagca ctaccttctt cctgcccacc agggacgtgc
      601 tgccgaaaac attgtttctc aagcttttat taaaagtggc gatgtaattc ctatgaatta
      661 ccactttaca acaacaaaag cccacatgga acttaacggg ggaacaattt ttgaaatatt
      721 caccgaggag gcactgaaaa tcaaaagcag ttttccgttc aagggaaata tcgacacaaa
      781 taagcttaac gaaatcattg ccaaatatgg caaagaaaga attccgttca tccgcttcga
      841 agcatcgacc aacttaattg gaggacaacc attctcgata cagaacatgc gtgacgtgaa
      901 gaaaattgcc gacgagcatg gaatcatgat tgtgctggat gccagcctta ttggcgagaa
      961 tgcttatttc attaaaaaga gagaagaaga atttaagaat accccgatta gcgaaatcct
     1021 aaaattgatg tgcggctttg ctgacgttgt atatttctcg agccgcaaag tgagctcgac
     1081 tcgcggagga ggtatttgca ccaacaaccg cgagttgtac atgaaaatgc gcgagctggt
     1141 gatcctctac gaaggctttt taacctatgg gggtatgtcg gtaagggaaa ttgaagccat
     1201 ggccgttggg ttgcgtgaaa caaccgacga aacagtaatc agccaatcgc cttcgtttat
     1261 tgaatatgcc gtaaacgaaa tgaataaact cggaatccct gtaataaccc ctgccggtgc
     1321 ccttggcgca catatcgact gcatgggatt tttaccccat gttccacaag aagaatatcc
     1381 tgcaggcgca ctggcagcag cactctttat tatttcagga atccgtggaa tggaacgggg
     1441 aaccatatcg agtgtgcgtg atgccaatgg caacgatgta ctttccgaca tggaattgtt
     1501 gcgcctcgcc ttccctcgcc gcgttttcac cctttcgcaa actatgtacg tgattgatcg
     1561 tgtaaactgg cttttccaca acagggattt agttggcgga ttgaaatttg ttgaagaacc
     1621 agccgtgctg cgtttcttta atggccgttt ggctccgacc agcaattggc cacagaaact
     1681 tgtcgccaag tttaaggccg actttggaga tagcttatag taagttagct taaatgcaat
     1741 gcggaaaatg aaataattcc cgtataatat atttttgatt ttttcccaat aaaaatgatt
     1801 ggctgttaga tacttcccgg atctttcagc catctttttt tcccttcaaa ggattttact
     1861 ccatcgacag gaaacggatc actttaatat tcgggtgg
//
LOCUS       DAOPUW010000405         1897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_66397_length_1897_cov_3.207383, whole genome shotgun sequence.
ACCESSION   DAOPUW010000405 DAOPUW010000000
VERSION     DAOPUW010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66397_length_1897_cov_3.207383"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PKJ43_07070"
     CDS             complement(<1..363)
                     /locus_tag="PKJ43_07070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNZ72365.1"
                     /translation="MKIISWNVNGIRAVAQKNFFDDFKALAPDILCLQETKAQDDQVA
                     ETLSPIEGYYVFSNSAEKKGYSGTAIISKTKPLSVTKGIGIEEHNNEGRVLCAEYENF
                     YLVTVYVPNSGSDLKRLDY"
     gene            592..1512
                     /locus_tag="PKJ43_07075"
     CDS             592..1512
                     /locus_tag="PKJ43_07075"
                     /inference="COORDINATES: protein motif:HMM:NF004846.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNZ72366.1"
                     /translation="MAKNWTFENIPDLQGKTIIVTGGNSGLGYESVKAFALKGATVVL
                     ACRNLAKGEKAKNEILKANPSGEIKVMQLDLASLASVEAFAKKFANEHKQLHVLLNNA
                     GIMATPNVKTVDGFEAQLGTNYLGHYALTGHLLPLLKATSKSRVVNVSSLAHKGGKID
                     FNDLMFEKGRKFKPMRAYAQSKLANLLFAYQLQRFFEANTIDCIAVAAHPGGSNTRLA
                     GHLETNRFVEAISKIARGAMQPAAKGALPQICASVDPNVKGGDYFGPHGLGELFGYPV
                     LVKSNAASHNEKNARKLWEVSEKLTGVKYA"
     gene            complement(1516..>1897)
                     /locus_tag="PKJ43_07080"
     CDS             complement(1516..>1897)
                     /locus_tag="PKJ43_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72367.1"
                     /translation="LVNWTLFKATFFVYETSLHYTYTIDYAMRMIFFLVLTLMVAYSC
                     NKDNDNNEEVKQLDGFVWISGGLATCAEQIHLVNGDTLIVRIEEFKTLKSGDKVNVKY
                     KELGANNSCPFGIDCEIIELKKIN"
BASE COUNT          554 a          365 c          401 g          577 t
ORIGIN      
        1 atagtcgagc ctttttaaat cgctgcctga gttgggtacg taaaccgtta caagatagaa
       61 gttttcgtat tcggcacaca acactcggcc ttcattgttg tgctcctcaa tgccaattcc
      121 cttggtgacc gacagtggtt tggttttcga aataatcgca gtacccgaat accctttttt
      181 ttcggctgag tttgagaaca cataataacc ttcaattgga gaaagggttt cggccacctg
      241 atcatcttgc gccttggttt cctgcaaaca aagaatgtcg ggtgcaaggg ctttgaaatc
      301 atcgaaaaag tttttctggg caacggcgcg tatgccgttc acgttccacg atatgatctt
      361 catttatatt tattttaagt tccggacaag tataacaggt ttcaaaaata attgtttttg
      421 aagagaatgg tgtaactgaa aactgtttga aaccgaacca caggtttttt tgttggtatt
      481 gtacaagtca gcaggttttt tctttttaga attccttttt ttaggctttc ttcacattat
      541 taaactattg tgtctttatt gtgttaattg gaacatttta gtgaatgagt tatggcaaaa
      601 aactggactt tcgagaatat tcctgacttg cagggaaaaa caattattgt aacaggtgga
      661 aatagcggac taggttacga gtcggttaaa gcttttgcac tgaaaggggc aacagttgtt
      721 ttggcttgcc gtaatttggc aaaaggcgaa aaagcgaaaa atgaaatatt gaaggctaat
      781 ccttctggtg aaattaaagt aatgcaactc gatttggcga gcctggcatc agttgaggca
      841 tttgccaaaa aatttgcgaa tgagcacaaa cagctccatg tattgctgaa caatgcaggc
      901 attatggcaa ctccaaacgt taagacagtt gatggtttcg aagctcagct gggcaccaat
      961 tatttaggac attatgctct taccgggcat ttgctacccc tgttgaaagc gacatccaaa
     1021 tcacgggttg ttaatgtaag cagtttagct cacaaaggag ggaaaatcga tttcaatgac
     1081 ctgatgtttg agaaaggacg gaaattcaag cctatgcgtg cctatgcgca atcgaagcta
     1141 gccaacctgt tgtttgctta tcagctacag cggtttttcg aagctaatac catcgattgt
     1201 attgccgtag cagcccaccc gggcggctct aatacccgtt tggctggtca tttggaaacc
     1261 aatagatttg tggaagccat ttcgaaaatt gcccgaggtg ctatgcaacc tgcagcaaag
     1321 ggagcgttac cgcaaatatg cgcttcagta gatccgaatg tcaaaggtgg tgactatttt
     1381 ggaccacacg gcctcggcga actatttggt tacccggttt tggtgaaatc aaatgcagca
     1441 tctcacaatg aaaaaaatgc cagaaaactt tgggaggttt cggaaaagtt gacaggcgtg
     1501 aaatatgcgt gagaattagt taattttttt tagctcaatt atttcacagt caattccaaa
     1561 tgggcaagaa ttatttgctc ccaattcctt gtactttacg ttcaccttgt cgcccgattt
     1621 caaggtttta aattcttcaa tgcgaacaat taatgtatca ccattaacca gatgaatttg
     1681 ctcagcacaa gttgctaatc cgccgctaat ccatacaaat ccgtcaagtt gttttacctc
     1741 ttcattatta tcattgtcct tattacaaga ataggcaacc ataagggtta gcacaagaaa
     1801 aaatatcatt ctcatagcgt aatctattgt ataagtataa tgcaatgatg tttcgtatac
     1861 gaaaaaggtt gctttgaata atgtccaatt caccaaa
//
LOCUS       DAOPUW010000406         1895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_66497_length_1895_cov_2.739130, whole genome shotgun sequence.
ACCESSION   DAOPUW010000406 DAOPUW010000000
VERSION     DAOPUW010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66497_length_1895_cov_2.739130"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="PKJ43_07085"
     CDS             complement(<1..1224)
                     /locus_tag="PKJ43_07085"
                     /inference="COORDINATES: protein motif:HMM:NF025861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 N-terminal
                     domain-containing protein"
                     /protein_id="HNZ72368.1"
                     /translation="MRTLIFSLWGFMVITYANAQQVSSPDGKLKINVASNEGKPSYNI
                     VYNDKTLILPSPVGLNTNVGDFTQGLTLSSEVKINKIDEIYSLPNIKKSQVHYMANEA
                     IFSFSKDNKPAFDLIFRVSNNNVAFRYMLHRQGERFVCMINQEATSFVLPQGTTTFLS
                     PQMDPMTGFARTAPSYEAPYTADDAMGKNGWGNGYIFPCLFRVNNDGWVLISETGVDS
                     RYCGSHLTGHENGLYTIAFPNEKENNGNGTSSPGIALPGFTPWRTITVGNNLSPIVET
                     TIPFDNVEPQYNASQRYEYTKGSWSWIMKMDGATKFEVQKQYIDFSASMGYKTILVDA
                     LWDTQIGYDKIEELAKYGASKGVSLYLWYNSNGYWNDAPQGPKDKMNQQSVRRKEMAW
                     MKSIGIKGIKVDFFGG"
     gene            complement(1417..>1895)
                     /locus_tag="PKJ43_07090"
     CDS             complement(1417..>1895)
                     /locus_tag="PKJ43_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ72369.1"
                     /translation="GSSWAWDDVHNQSNESTMFYTLEPGNHTLYICYRENGAAIDKLY
                     ITNTGKVPSGLGGEASSCPEIEEPEIPNGFQPFVANNTIKVFPNPTKSKINIESLKPL
                     TSLNIYNLNGLRVYSKTYSDETFNEEIELHIEAGIYFLQTITEKNYSIQKLIIENQ"
BASE COUNT          464 a          373 c          399 g          659 t
ORIGIN      
        1 tccaccgaag aaatcgactt taattccttt aattccaatg ctcttcatcc aggccatttc
       61 tttgcgtcgt acagattgct ggttcatttt gtctttcggc ccttgtgggg catcgttcca
      121 gtatccgttt gaattgtacc ataaatacag tgaaactcct tttgatgctc cgtatttagc
      181 taattcttca attttgtcgt atccaatttg tgtatcccag agggcatcaa ccagtattgt
      241 tttgtacccc atagatgcac taaaatcgat atattgcttt tgaacttcga atttggttgc
      301 accatccatt ttcataatcc agctccatga accttttgtg tattcgtatc tttgtgatgc
      361 attatattgt ggttcaacat tgtcgaatgg aattgttgtt tcaacaatag gggagaggtt
      421 gttgccaacg gtaattgttc gccatggggt gaaacccggt aaagcaattc ctggtgatga
      481 cgttccattg ccgttgtttt ctttctcatt ggggaaagct atggtataca gcccgttttc
      541 atggcctgta aggtggcttc cgcaataccg gctgtcgaca ccggtttctg aaatcagtac
      601 ccaaccatcg ttgtttaccc tgaacaggca agggaaaatg tagccattgc cccaaccgtt
      661 tttgcccatg gcatcgtcgg cagtgtatgg tgcctcgtag ctcggtgcag tgcgtgcaaa
      721 gcctgtcatt ggatccattt gcggagaaag gaaggtggtg gttccttggg gcaaaacaaa
      781 gctagtggct tcttggttaa tcatgcatac aaagcgttct ccttggcgat gaagcatata
      841 tctgaaggca acgttgttgt tgcttactct gaaaatcagg tcgaaagcag gtttgttgtc
      901 tttggaaaat gaaaagattg cttcgttagc catataatgc acctgacttt ttttgatgtt
      961 gggcaacgag tatatttcgt caattttatt gattttcact tcgctgctca gtgtaaggcc
     1021 ttgggtaaaa tcgcctacgt tagtgtttaa tcctactggt gacggcagaa tcagggtttt
     1081 gtcattgtaa acaatgttat aacttggttt tccctcattg gacgcaacgt ttatcttaag
     1141 ctttccatct ggactggaaa cttgttgcgc atttgcataa gttataacca taaagcccca
     1201 aagggaaaat attaatgttc tcactatttt tatgcttgct tctctcatcg ttattgttag
     1261 ttaatgtttt ctcaaatata aaattcaatc aaatttttct ctggctttta aaaaaatcgc
     1321 agtagaacga gtttccttga tttattactt tgccaaagta caaaaaaaac cggcgccagg
     1381 ctagactacc tatgcgcacg gtttcctgct ttctgtttat tgattttcta ttattaactt
     1441 ttgtatcgaa taattttttt cggttatggt ctgaagaaaa taaatccctg cttcgatatg
     1501 aagctcaatt tcttcgttaa atgtttcatc tgaatatgtt tttgaataaa cccgaaggcc
     1561 gttgaggttg taaatattta acgatgtaag gggtttgagg ctttcaatat tgatttttga
     1621 ctttgtaggg tttgggaata cttttattgt gttgtttgca acaaatggtt gaaatccgtt
     1681 gggaatttcg ggttcttcaa tttcgggaca agagcttgcc tcgccgccta agccggaagg
     1741 aaccttacct gtattggtta tatatagttt gtcgatggct gcgccatttt cccgatagca
     1801 gatataaagg gtgtggtttc caggttccag cgtataaaac atcgtgcttt cattactttg
     1861 gttatgcaca tcatcccatg cccaactgct gcctc
//
LOCUS       DAOPUW010000407         1893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_66587_length_1893_cov_3.619695, whole genome shotgun sequence.
ACCESSION   DAOPUW010000407 DAOPUW010000000
VERSION     DAOPUW010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66587_length_1893_cov_3.619695"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            564..>1893
                     /locus_tag="PKJ43_07095"
     CDS             564..>1893
                     /locus_tag="PKJ43_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNZ72370.1"
                     /translation="MKNYKSEQLRNIALIGNAGAGKTTLAEAMLFEGGVLNRRGEVTS
                     KNTASDYTTIEQDYGNSLFSTLLYTEYEDKKINIIDTPGMRAFAGAVTSSLSVVDAAV
                     CVLNSADGIGAGTEGAMRIAQAMKIPVIFLANGMDHENVNFDQLIENAIAKYGHSVTV
                     VQYPVNAGPGFNAIIDVLKMKMYQYGPDGGAPQVLDIPASEKDKADELHNALIEKAAE
                     NDEKLMELFFEQGSLSEEEMRVGMRLGIKDCTLYPVFCASAKKNMGVKRFMEFIVNIV
                     PSPIMATPRTEMSGKAVKCDAAGPASLFVFKTSIEQHIGEVTYFKVMSGTITEGIDLV
                     NTTKQGKERMAQLFAVAGKTRNKVTELVAGDLGATVKLKETKVNHTLSVKEADYSFAP
                     MEFPKSLITTAIKAVNEADDEKLAQVLHRLRDEDPSFKVYHSKELKQLIVE"
BASE COUNT          608 a          337 c          413 g          535 t
ORIGIN      
        1 ataagccatt ccggttgcat atactgtgct ttcggtaaca attgctgcgg taagcatttt
       61 tctgttcaca ggaggcggtt tcaccgagtt gggtaatggt tggcaggtcg ctgttataca
      121 aataattgtc aaaacaatgg ttagataatg ccgaatgctc atggtcgtga tctttatact
      181 aagtaaccga aaattcaaga ataattcaat tgcaggtata attaaaaatg tgttcttctg
      241 ctttttttag gttgaattga ataaagcttt agtattttat acttctttaa caaattaagt
      301 ttacgttatc agaaaaaaag ttgattagcc agaatgtgtt gtgatttaga gaataagaaa
      361 gggtttaagc acaaaacgat ctgacattca ggggcgagta taaaaacaaa ctatgtgcta
      421 actatcgttt ataaaagaag ttagcttgag aatgcctaat gaatcagttt agttacacaa
      481 tacttggttg ttatcaaatt attattattt ttgaagttct caattgaaaa atcaacgttt
      541 acagaacaaa tacactaaaa gttatgaaaa attataaatc agaacaacta aggaacatag
      601 ccctgattgg taatgctggc gctgggaaaa ccaccctcgc tgaagcaatg cttttcgagg
      661 gtggtgtcct aaaccgcaga ggtgaagtaa ctagtaaaaa cacggcctcc gactatacaa
      721 ccattgaaca agactacggc aactctctct tctcgaccct cctttataca gaatacgaag
      781 acaaaaaaat taatatcatc gataccccgg gaatgcgcgc atttgccgga gcggtaactt
      841 cgtcgcttag tgttgtcgat gctgcagttt gtgtactcaa ctctgctgat ggtattggag
      901 caggaaccga aggggctatg cgtatagctc aggcgatgaa gattcctgtg atcttccttg
      961 caaacgggat ggatcatgaa aacgttaatt tcgaccaact gattgaaaat gccatagcta
     1021 agtatggtca cagtgttact gtggttcagt atccagttaa tgcgggtcct ggttttaatg
     1081 ctatcatcga tgtgctgaag atgaaaatgt accaatatgg ccccgatggt ggtgctcctc
     1141 aggtacttga tatcccagct tcagaaaaag ataaagccga tgaattgcac aatgctttga
     1201 ttgaaaaggc agccgagaac gacgaaaaac tgatggaact tttctttgag caaggatcgc
     1261 tatctgaaga agaaatgcgg gtaggaatgc gcttaggtat aaaagactgt acgttgtacc
     1321 ctgttttttg tgcatcggca aagaaaaaca tgggtgtaaa acgttttatg gaattcattg
     1381 ttaatattgt accctctccc ataatggcaa cgccacgaac tgaaatgtcg ggtaaagccg
     1441 tgaagtgtga tgctgcaggc ccagcttcgt tatttgtttt caaaacaagc attgaacagc
     1501 atattggaga ggttacttac tttaaagtaa tgtcgggtac aattaccgaa ggcattgacc
     1561 ttgtcaatac aaccaaacaa gggaaggagc gaatggcaca attgttcgct gtagccggaa
     1621 aaaccagaaa taaagtaact gaattggttg ctggcgatct tggagctacg gttaaactga
     1681 aagaaaccaa ggttaatcat acacttagtg tcaaagaagc cgattatagt tttgctccta
     1741 tggaatttcc aaagtcattg ataactactg cgattaaggc cgttaacgaa gccgacgatg
     1801 aaaaacttgc tcaagttttg caccgtttac gcgacgaaga tccatctttc aaagtatacc
     1861 attcaaaaga attgaaacag ttgatcgttg aag
//
LOCUS       DAOPUW010000408         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_66699_length_1891_cov_3.258715, whole genome shotgun sequence.
ACCESSION   DAOPUW010000408 DAOPUW010000000
VERSION     DAOPUW010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66699_length_1891_cov_3.258715"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PKJ43_07100"
     CDS             <1..286
                     /locus_tag="PKJ43_07100"
                     /inference="COORDINATES: protein motif:HMM:NF017024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNZ72371.1"
                     /translation="AITLAEGIINQRGIYDGWVNEQTDDEKLLGIVSIREGDVPGIHN
                     VCYDSDDDYIEIVHSARNRKGFAFGAVLAAEFLPGKTGIYSMNDLLKFDE"
     gene            401..1744
                     /gene="lepB"
                     /locus_tag="PKJ43_07105"
     CDS             401..1744
                     /gene="lepB"
                     /locus_tag="PKJ43_07105"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNZ72372.1"
                     /translation="MKKILSNKWVKFSFWGLLYLLWVIWLGNYWFLLGLPIIFDIYVS
                     KKVHWAFWKKKNPPNGKQTKTVEWIDAIIFAVIAASFIRLFFFEAYVIPTSSMEKSMM
                     VGDYLFVSKVAYGPKMPNTPLSFPFVHQSMPLTNYKVNSYVEWIKRPYNRLGGLSKIK
                     NDDVVVFNFPEGDTVALNFPSQNYYELCRMYGRNRVISDERNFGKIVSRPVDKKENYI
                     KRCIGIPGDKLEIIDGDVFVNGKKQKEHPGKQYNYIVTTDGSLFNQKVLDKLKIANDD
                     REMFSGGKYMFPLTQKQLTEIKALKNVTSVEKYIAPKGTYDPSIFPHSPNFSWNVDNF
                     GPLEIPKKGNTIELSLTNLPIYERIIDVYEQNDLAVRNGQIYINGQVATSYTFKLDYY
                     WMMGDNRHKSADSRYWGFVPVDHVVGKASLIWMSLDKDKKFPSKIRFNRIFKTVK"
BASE COUNT          593 a          344 c          391 g          563 t
ORIGIN      
        1 cgccatcaca ttagccgaag ggattattaa tcaacgcggt atttatgacg gttgggtgaa
       61 cgagcaaacc gacgatgaaa aactgttggg aattgtgtcg atacgtgagg gcgatgtgcc
      121 gggcatccat aatgtttgtt acgattcaga tgacgactat attgaaatag tgcacagtgc
      181 acgtaaccga aaaggttttg catttggtgc cgtgttggca gccgaattcc ttccggggaa
      241 aaccggtatt tactcgatga atgatttatt gaaatttgac gagtaagaga tcaaaaataa
      301 ccgagccaag agcttgtcgc atgtaaacca ttcattatat ggcaataacg atagtatctg
      361 gcaccatcaa atattcaaaa agaattaaaa aataattgag atgaagaaaa tactgagcaa
      421 taaatgggtt aaattctcct tttgggggct tttatatctt ctgtgggtta tatggctcgg
      481 aaactattgg tttctgcttg gcctgcctat aatttttgat atctatgtgt cgaaaaaagt
      541 tcattgggct ttctggaaaa agaagaatcc acctaacggt aaacaaacca agactgttga
      601 atggattgat gccataattt ttgcggtgat tgcagcttct tttattcgtc tgtttttctt
      661 cgaagcatat gtaattccta cgtcttccat ggaaaaatct atgatggttg gcgattacct
      721 tttcgttagt aaagtagcat acggtcctaa aatgcccaat actccattat cgtttccttt
      781 tgtacatcag tcgatgccgc taactaatta taaggtaaat tcttacgttg aatggataaa
      841 acggccatat aatcgattgg gaggtcttag caaaataaaa aacgatgatg ttgttgtgtt
      901 caatttcccc gaaggtgata ctgtggcatt gaattttccc agccagaatt attacgaact
      961 ttgccgcatg tacggacgaa acagggttat aagtgacgaa cggaattttg gaaaaattgt
     1021 cagccgccct gtcgacaaaa aagaaaacta tattaaacgc tgtattggta tacccggcga
     1081 taagcttgaa attatcgatg gcgatgtttt tgtaaatggc aaaaagcaga aagaacaccc
     1141 cggcaagcaa tacaactaca ttgtaaccac cgatggctct ctctttaatc aaaaagtact
     1201 tgacaaacta aagattgcaa acgatgaccg cgaaatgttt tcgggcggaa aatacatgtt
     1261 cccgttaaca caaaagcagc ttaccgaaat taaggctctt aagaatgtta cctcagttga
     1321 aaaatatatt gcacctaagg gtacctacga tccatcgatc ttccctcata gtccaaattt
     1381 ttcgtggaat gtcgataatt tcggaccact cgaaattcct aagaagggaa acacaattga
     1441 attgagcttg acaaatttac ctatatacga gcgaattatc gacgtgtatg aacaaaacga
     1501 tcttgccgtt cgtaatggac aaatctatat taacggacag gttgctacca gctatacttt
     1561 taagctcgat tattattgga tgatggggga caatcggcat aaatcggctg attcgcgcta
     1621 ttgggggttt gtacctgtcg accatgttgt tggaaaggca tcgcttattt ggatgtcgct
     1681 tgataaggat aaaaaattcc cttcaaaaat aaggtttaac cggatattca aaaccgtaaa
     1741 ataaaatcag ggtacttcgg tacccttttt tatttttgca actataaaca gttactgttt
     1801 ttactatgca atcaattctt ttatcaacgg cttatcttgg gccggtaagt tatttctcgg
     1861 caatttcacg agccgacaaa gtaattattg a
//
LOCUS       DAOPUW010000409         1887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_66926_length_1887_cov_2.696507, whole genome shotgun sequence.
ACCESSION   DAOPUW010000409 DAOPUW010000000
VERSION     DAOPUW010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66926_length_1887_cov_2.696507"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PKJ43_07110"
     CDS             complement(<1..475)
                     /locus_tag="PKJ43_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72373.1"
                     /translation="MKKNADLIKWNVTPEVLETHAGQVKVAVDGQIPAKYFAKKATVV
                     VTPVMKFEGGEVAYPEIKLQGEKVKANNAVITYKPGGNFSIKGAIPYEQAMKMSELQV
                     RIVASKGAASLDFDPIKIADGVIATSELVNKVGEPIIGIQREKNTTGKYDPAIDPF"
     gene            complement(654..1283)
                     /locus_tag="PKJ43_07115"
     CDS             complement(654..1283)
                     /locus_tag="PKJ43_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNZ72374.1"
                     /translation="MNFLVIEGNIGAGKTTLAQMISSDYNAQLVTESFAGNPFLPKFY
                     ANPSTYAFTLEMSFLAERYSQLMNELQNRDLFKDFAVADYYFMKSLIFASVTLSDDEF
                     TLYRKFFNLIYARLPRPDLYVYLHKDTDVLQKMIKKRGRDYESNIADEYLDNITKAYF
                     NYFKQQSEFPVVIIDTTNIDFVKNDKDYLKICDTIFQNSYSSGITRVIL"
     gene            complement(1296..>1887)
                     /locus_tag="PKJ43_07120"
     CDS             complement(1296..>1887)
                     /locus_tag="PKJ43_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010424254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNZ72375.1"
                     /translation="GGGEYGESWHQGGTLFNKQNVFDDFIAGAEYLIGSGYTNRDKLS
                     IRGGSNGGLLVGAVTNQRPDLFKVALPAVGVLDMLRYQKFTIGYAWATDYGTADDSEE
                     MFRYLLAYSPYHSVEKKDYPAILATTADHDDRVVPAHTFKYISRIQELNTGKLPTLVR
                     IDVKAGHGSGKPTAKVIEEYTDIYSFVFYHTGITLK"
BASE COUNT          528 a          409 c          378 g          572 t
ORIGIN      
        1 ggaatgggtc aattgcagga tcatattttc ctgttgtgtt cttttcacgt tggataccaa
       61 tgattggctc accaacttta ttaaccaatt ctgaggtagc aataacgcca tcagcaatct
      121 taataggatc gaaatcgagt gatgcagcgc ctttcgatgc tacaatacgc acttggagtt
      181 ccgacatttt cattgcctgt tcgtaaggaa ttgccccttt gattgagaaa ttaccgcctg
      241 gtttgtaggt aattacagca ttgttagctt ttaccttttc tccctgaagt ttaatttcgg
      301 ggtaagcaac ttctccacct tcgaatttca taacaggagt aactactacg gtagctttct
      361 tggcaaaata ttttgcggga atttgaccgt cgacagcaac ttttacctga ccggcatgag
      421 tttcgagtac ttcaggagtg acgttccatt taatgaggtc agcatttttt ttcatctttt
      481 tgatgcttgc acaactgcta aagagcacaa ttacaagagc aaaagagact agagctttaa
      541 agttaatttt tttcatgatg gacacattat tattattttt caactatatt tcactaatta
      601 ctacaaaaat aagaatgttt ttcatatcaa aaagccttga tacaaaaatt ttatcataat
      661 ataacccgtg taatgcctga gctgtaacta ttttgaaaaa ttgtatcaca aattttcagg
      721 taatccttgt cattttttac aaaatcgata ttcgttgtat ctataataac aacagggaat
      781 tcactttgtt gcttaaagta gttaaaataa gcttttgtta tgttatcgag atattcgtcg
      841 gcaatattgc tttcgtaatc acgccctctt ttttttatca ttttttgcaa aacatcggta
      901 tctttatgca gataaacgta taaatcgggc cttggaagcc gggcataaat caggttaaag
      961 aacttgcggt acaaggtgaa ttcgtcgtca gaaagggtta cgctggcaaa aatcagcgac
     1021 ttcataaagt agtaatcggc tacagcaaaa tctttgaaca aatcgcggtt ttgcagttcg
     1081 ttcatcagtt gcgaatagcg ttcggcaagg aacgacattt cgagcgtgaa agcgtaggtc
     1141 gaagggttcg cgtaaaactt gggcaaaaaa ggattgcccg cgaaactttc ggttaccaat
     1201 tgggcgttat aatcgctgct gatcatttgc gcaagggtag ttttcccggc gccaatgttt
     1261 ccttctatca ccaaaaaatt cattgccctg cctctttatt tcaacgtaat acccgtgtgg
     1321 taaaacacaa atgaatatat atcggtatat tcttcaatca ccttcgccgt aggctttcca
     1381 ctgccgtgac cggctttcac atcgatgcgg acgagggtgg gcaacttccc ggtattcaac
     1441 tcctgaatac gcgaaatata tttgaaggtg tgggcaggaa ccacccggtc gtcgtggtcg
     1501 gccgtagtcg ccaaaatagc cggataatct ttcttttcga cactatgata gggtgaatag
     1561 gccaacaaat accggaacat ttcttcgctg tcatcagcag tcccataatc ggtagcccaa
     1621 gcgtacccta tggtaaattt ctggtaacga agcatatcga gtacacctac ggcaggaagt
     1681 gccaccttga acaaatcggg acgttgattg gtaactgccc ccacgagcaa gcctccattt
     1741 gaaccacccc tgatgctcag tttatcgcgg ttagtatacc cgctcccgat aaggtattca
     1801 gcaccggcaa taaaatcgtc gaacacattt tgcttgttga acaaggtgcc gccctgatgc
     1861 cagctttcac catactcacc ccctccg
//
LOCUS       DAOPUW010000410         1885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_67063_length_1885_cov_2.363934, whole genome shotgun sequence.
ACCESSION   DAOPUW010000410 DAOPUW010000000
VERSION     DAOPUW010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67063_length_1885_cov_2.363934"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="PKJ43_07125"
     CDS             <1..1225
                     /locus_tag="PKJ43_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNZ72376.1"
                     /translation="TQQEAYDAVRYGFEISEKFSLPVMMRVTTRMSHSRSGIARSKVL
                     QQNAINFPSDPMQFMLLPAFARKQYHKLIEIQGELERESENSKLNKIEINNKGGIGVL
                     ACGIGYNYLLENIAQTRSSYNILKVSQYPLPRNMVEQLFANSSKVLVLEEGYPIYEEL
                     LRGYLNSATAVPIDGRLNGVVVREGELDPDNVALALGKKASQPESIDGIVKNRPPQLC
                     QGCSHHDMYEALNEVLAGFPSKKVFADIGCYTLGALPPLKAIDTCVDMGASITMAKGA
                     ADAGFRPSVAVIGDSTFTHSGITGLLDCVNDQSAVTIIISDNSTTAMTGGQDSSGTGR
                     LVEICVGLGVDPDHIRVIEPLGKNFEANVDILREEVNYEGVSVILAQRECIQTQLRRK
                     KTEKKDDPLVRNCKH"
     gene            1232..1810
                     /locus_tag="PKJ43_07130"
     CDS             1232..1810
                     /locus_tag="PKJ43_07130"
                     /inference="COORDINATES: protein motif:HMM:NF005324.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HNZ72377.1"
                     /translation="MKKDIILAGVGGQGILSIAAVIDSAALSLGYKVKQAEVHGMSQR
                     GGAVQSHLRISDAEIYSDLIGLKSADLIISVEPMESLRYIPFLSENGWVVTSRDCMKN
                     IANYPEEEELFAQIEKLPRKIAVDAEKLAREAGSAKASNIVMLGVAAPFLGIAIDKLK
                     KAIEVIFADKGQKIIDINLRAFEAGLACNSNK"
BASE COUNT          545 a          369 c          439 g          532 t
ORIGIN      
        1 gactcaacaa gaagcatacg atgcagtgcg ttatggtttc gaaatttcag agaaattcag
       61 tttacccgtc atgatgaggg ttaccacccg tatgtcgcac tcccgttccg gaattgcccg
      121 ttcgaaggtt cttcaacaaa acgcaatcaa ttttccatca gaccctatgc agtttatgct
      181 tttgcccgct tttgcccgca aacaatatca taaactcatc gaaattcaag gagaattgga
      241 aagagagtct gagaattcaa aactcaataa gatcgaaatt aacaataaag gggggattgg
      301 tgtactggca tgtggcattg ggtacaatta cctgcttgaa aatattgctc aaacccgttc
      361 atcctacaat atcctgaaag tatcacaata cccgcttccc cggaacatgg ttgaacagct
      421 ttttgccaat tcatcaaaag ttcttgtgct ggaagaaggt tatccaattt atgaagaact
      481 gttacgcgga tatttaaatt ctgccactgc agtgcctatc gacggacgct tgaatggtgt
      541 tgttgttcgc gaaggtgaat tagatcccga caatgttgca cttgccttag gtaaaaaagc
      601 atctcagcct gaaagtatcg acggaattgt taaaaacaga ccgccacagc tatgtcaggg
      661 atgtagccac catgatatgt atgaagcgtt gaatgaagtt ctcgctggtt ttccttccaa
      721 aaaagtgttt gccgatattg gctgctatac gcttggggct cttcctccat tgaaagcaat
      781 tgacacttgt gttgacatgg gggcatccat aacaatggct aagggagccg ccgatgcagg
      841 ttttcgtcct tcggtggccg ttattggcga ttcaacgttc acccactcag gtattacagg
      901 tcttctcgat tgtgtcaatg atcaatctgc tgtgactatt atcatttcag ataactcaac
      961 aactgccatg accggcgggc aagattcttc gggaacaggc cgtctggtag aaatttgtgt
     1021 tggtttgggt gtcgatccag atcatatccg tgtaattgaa ccgttgggga aaaacttcga
     1081 agcaaacgta gatattcttc gtgaagaagt taattatgag ggtgtttctg taattctggc
     1141 tcagcgcgag tgcattcaaa cccagttgcg cagaaagaaa accgagaaaa aagacgaccc
     1201 attggtaaga aactgtaaac attaatacga aatgaagaaa gacataatat tagccggagt
     1261 aggcggtcag ggaattttat ctattgcagc agtgattgac tctgcagctt tgtcgcttgg
     1321 atataaagtt aagcaagccg aggttcatgg catgagccaa cgtgggggtg cggtacagtc
     1381 acatcttcgc atctctgatg ccgaaattta cagcgacctt attggattga aaagtgccga
     1441 tttgatcatt tctgttgagc ccatggagag cttaaggtat ataccttttt tgtcggagaa
     1501 tggctgggtg gtaaccagtc gcgactgtat gaaaaacatt gccaattatc ctgaggaaga
     1561 ggaattgttt gctcaaattg aaaagttgcc aagaaaaata gctgtagatg cagaaaaatt
     1621 agctcgtgaa gcaggttcgg ccaaagcatc gaacattgtt atgctggggg ttgccgctcc
     1681 gtttttaggt attgcaatcg ataagctaaa gaaggcaatt gaagtaattt tcgccgacaa
     1741 agggcagaaa ataatagaca ttaacttacg cgcgtttgaa gcaggcctgg cttgcaacag
     1801 taacaaataa tattgacttg cttcctgcct tattttttat aaggcgggaa gatttttaca
     1861 aatgtgtttt ttagattttt ttcat
//
LOCUS       DAOPUW010000411         1882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_67203_length_1882_cov_2.623426, whole genome shotgun sequence.
ACCESSION   DAOPUW010000411 DAOPUW010000000
VERSION     DAOPUW010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67203_length_1882_cov_2.623426"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..623
                     /locus_tag="PKJ43_07135"
     CDS             186..623
                     /locus_tag="PKJ43_07135"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNZ72378.1"
                     /translation="MATLNFSTKGVSENPTKFNAQSRTFKIVVDEPEQLGGTNHGANP
                     VEYILAGYAGCLNVVGHLVAKEQNLKLNKLSIEIDGDINPDRFLGVSDNDRAGFKIIR
                     VKITPDTTASKEEIEKWINEVEKRCPVNDNLQNPTPIHIELAR"
     gene            693..1652
                     /gene="cysK"
                     /locus_tag="PKJ43_07140"
     CDS             693..1652
                     /gene="cysK"
                     /locus_tag="PKJ43_07140"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNZ72379.1"
                     /translation="MTQIAKKLTDLIGKTPLLELSNFNKENGTLGTIIAKLESFNPGG
                     SVKDRIGFAMIEAAEKQGILQKGTVIIEPTSGNTGIALAYIAASKGYRLILTMPETMS
                     IERRSLLKALGAELVLTPGPEGMKGAIKKAEELHLSISNSIIPQQFKNPANPEIHRKT
                     TAEEIWNDTDGNVDVFVSGIGTGGTITGVGEVLKARKPSVKIIAVEPYDSPVLSGGNP
                     GPHKIQGIGAGFVPPILNTSIFDEVIKVRNDDAFFASRELARTEGLLVGISSGAAAWA
                     ALQVSLRSEFAGKNIVVLLPDTGERYLSTVLYQPEEATQTTAK"
BASE COUNT          630 a          418 c          407 g          427 t
ORIGIN      
        1 ttttccattg ttttcgtttt atgtgttatt ccttaaaaat tgattataag aataatgcct
       61 aatttatcca tttacgtaac caacatccca catttacggt agataaatta ccctatctgc
      121 gggattttca gtgaactatt gcctgcgtag ttttgcctta tgaaatcata taaaacaaac
      181 tacatatggc aacattaaat ttttcgacaa agggtgtaag cgaaaaccct acaaaattca
      241 acgcacaatc gagaaccttc aaaatcgtgg tcgatgaacc ggaacagctt ggtggaacca
      301 atcatggggc taacccggtt gaatatatcc tggccggtta tgccggatgc ctcaacgtgg
      361 tagggcattt ggtcgcaaaa gaacaaaacc tgaagctcaa caagctttcc attgagattg
      421 atggcgacat aaatcccgac cgttttttag gagtttcgga taacgaccgt gccggattca
      481 agataataag ggtaaaaatc acccccgaca ccactgccag caaagaggaa atagaaaaat
      541 ggattaacga agtagagaaa agatgtccgg tgaacgataa cttacagaac cccaccccta
      601 tccatataga attagcacgc taaaaattaa ttccttgaat aattcgagca attcacagaa
      661 agtaaaaaag gaaaatttta aaagaaaaaa acatgacaca gatagcaaaa aaattaaccg
      721 atttgatagg caaaaccccg ctattggaac tatcgaattt taataaagaa aatggtacgc
      781 ttggaaccat cattgccaag cttgaatcgt ttaaccccgg cgggagtgtg aaagaccgaa
      841 ttggctttgc catgatcgag gcagccgaaa agcaaggaat attgcagaaa ggtacggtaa
      901 ttattgagcc caccagcgga aacacgggta ttgcactggc ctacattgcc gcctcgaaag
      961 gctacaggct tatcctaact atgcccgaaa ccatgagcat tgaaaggcgc agtttactca
     1021 aagccctcgg ggccgaactg gtgcttaccc ccgggccgga agggatgaaa ggcgccatca
     1081 aaaaagcgga agaattacat ttatccatca gcaattctat tattccacag caatttaaaa
     1141 acccggcaaa ccccgagatt caccgtaaaa ctacggccga agaaatatgg aacgacaccg
     1201 atggaaatgt agatgttttc gtgagcggaa ttggaacagg aggaaccata acgggtgttg
     1261 gcgaggtact taaggcccgt aagccctcgg tgaaaatcat tgccgttgag ccctacgatt
     1321 cgccggtact ttcgggcggg aacccgggac cacataaaat acaagggatc ggagcaggat
     1381 ttgttccgcc aatactgaac acctcgatat tcgacgaagt gataaaagtg cgaaacgatg
     1441 atgctttttt tgcaagccgt gaactggcgc gcaccgaagg gctgctggta ggcatttcgt
     1501 cgggagctgc agcgtgggct gccttgcagg taagcctccg cagcgaattt gccggaaaaa
     1561 atattgtggt gctcctgccc gacacgggcg aacgttacct atcgacagta ctataccaac
     1621 ccgaagaagc aacacaaacc accgcaaaat aaacacattt aaaaagcaag ttcctaaaat
     1681 gcgagcatcg ctcaaaaatg agcatacggt gactctgaca ggagattcct cattccgaaa
     1741 gtagtgagga atcttaacat ttatacttgt catttgtatc ctccagttgg cagaaaacaa
     1801 tcaaaatttc aaccagacaa ccaatgatga taaacattca ttaacgccac gacgttaata
     1861 agaccagcct ataaagcaaa aa
//
LOCUS       DAOPUW010000412         1879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_67366_length_1879_cov_2.728618, whole genome shotgun sequence.
ACCESSION   DAOPUW010000412 DAOPUW010000000
VERSION     DAOPUW010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67366_length_1879_cov_2.728618"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /locus_tag="PKJ43_07145"
     CDS             <1..1462
                     /locus_tag="PKJ43_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HNZ72380.1"
                     /translation="ISMRLFTSLAHEDVNVILISQASSENSISVAIPTIAAEKAAKAV
                     DTEFQKEIEAGLVSSVKVENELSIVAIVGENMRESAGIAGKLFNTIGKNGINVRAIAQ
                     GASELNISWVVKTSDLRKTLNVVHESFFLTSYKELNLFLMGIGTVGGNFIKQLQQQQE
                     KLFNEKRLKLKLVGVANSKKMVFDRDGIELENYKPLLKESSSVSTFDAFVQQMKDMNM
                     FNSVFIDCTANEDLAEYYLSILESSISIVAANKVAASSTYSYYKQLKDTAVEKGIKFY
                     FETNVGAGLPILSTIKDLINSGDRILKIEAVLSGTLNYIFNTISSSIPLSKAVLMAKE
                     EGYSEPDPRIDLSGKDVVRKLLILCREAGYSIEEEQVAVETFIPNALMTGSIDEFWNG
                     LKLLDTEFEDKRRRLEAENKKWRFVAQYDNSGSRIALREVDSQSPFFDLEGSNNIILF
                     TTERYNEYPMQIKGYGAGAGVTAAGVFADIIRVANI"
     gene            1574..>1879
                     /locus_tag="PKJ43_07150"
     CDS             1574..>1879
                     /locus_tag="PKJ43_07150"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HNZ72381.1"
                     /translation="MTEQMPVKKNILVQVSVIDALLQGIYDGIMPIGELLEYGDLGIG
                     TFHGLNGEMVVLNDTVFQVLSTGAVAKPASDVSTPFAAITAFSADTTFALEDISYDSL
                     "
BASE COUNT          556 a          326 c          438 g          559 t
ORIGIN      
        1 tatttctatg cgtttgttta cctcgttggc tcatgaagat gtcaacgtaa tcttaatttc
       61 tcaggcatcg agtgaaaatt ctatctcggt agccattcct actattgctg ctgaaaaggc
      121 tgcaaaagct gtagatactg agtttcagaa agaaattgag gcaggattgg tcagtagcgt
      181 taaagttgaa aacgaacttt caattgtagc cattgtaggt gaaaatatgc gtgaaagtgc
      241 aggaattgct ggtaaactgt tcaatactat tgggaaaaac gggattaatg ttagggctat
      301 tgctcaaggg gcttccgaat tgaatatttc ctgggtggta aaaaccagtg atttacggaa
      361 aacattaaat gttgttcacg agagcttttt ccttacttca tacaaagagt tgaatttgtt
      421 tctgatgggc attggtactg ttggcggaaa tttcatcaaa caattacaac agcaacagga
      481 aaagttgttc aacgagaagc gattgaaact aaaattggta ggggtagcca acagtaaaaa
      541 aatggttttt gaccgcgatg gaatcgaact cgaaaattat aagccgttgc tcaaagaatc
      601 ttcatcagtt tcaactttcg atgcctttgt tcagcaaatg aaagacatga atatgtttaa
      661 ctcagtcttt attgattgta ctgcaaatga agatcttgcc gaatattatt tgtcaatact
      721 tgagtcgagt atttccattg tggcagccaa taaggtggcc gcttcttcaa catatagtta
      781 ctataaacaa ctgaaggata cagcggtaga aaaaggaatc aagttctatt tcgagacaaa
      841 cgtgggggca ggccttccaa ttttatctac gattaaggat ttgattaaca gtggcgaccg
      901 cattctgaaa attgaagcgg ttctttcagg aacattgaat tatatcttca atacaattag
      961 ttctagtatc cctcttagca aggccgttct tatggcaaaa gaagagggat actccgaacc
     1021 cgatccccga atcgatctta gtggaaaaga tgtggtgcgg aagttgctga ttctttgccg
     1081 cgaagctgga tatagtattg aagaagagca agtagctgta gaaacgttta ttccaaatgc
     1141 tctgatgaca ggttcgatcg atgagttttg gaacgggctc aagctgctcg ataccgaatt
     1201 cgaagacaaa cgacgtagac tcgaagcaga gaataaaaaa tggcgttttg ttgcgcaata
     1261 tgacaattca ggttcgcgaa tcgcacttcg tgaagttgat tcacagtcgc cattcttcga
     1321 tcttgaagga agtaataata ttattttatt cacaactgaa cggtacaatg agtatcctat
     1381 gcagataaaa gggtatggcg ccggcgcagg agtgactgca gcgggggtat ttgccgacat
     1441 tatccgggta gcaaatattt agatatcctt gtgctgaagt taatatattg cggtttaact
     1501 gtatagtgtg atgaaaaagc ttttgttatc cttatccgtc tttacttttt tgtgggcttg
     1561 taacagcgaa aaagtgaccg agcagatgcc ggttaagaaa aatattctgg ttcaggtttc
     1621 ggtgatcgat gccttgctgc aaggaattta cgatggaatt atgccaattg gggaactttt
     1681 ggaatatggc gacctcggta ttggtacctt tcacgggctg aacggggaga tggtcgtgct
     1741 gaacgatact gtttttcaag tgctatcgac aggtgctgtt gcaaaacctg catctgatgt
     1801 ctcaacccct tttgctgcaa taactgcatt cagtgccgat acaacatttg cccttgaaga
     1861 cattagttac gatagtttg
//
LOCUS       DAOPUW010000413         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_68015_length_1868_cov_2.047435, whole genome shotgun sequence.
ACCESSION   DAOPUW010000413 DAOPUW010000000
VERSION     DAOPUW010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68015_length_1868_cov_2.047435"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            393..1580
                     /locus_tag="PKJ43_07155"
     CDS             393..1580
                     /locus_tag="PKJ43_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HNZ72382.1"
                     /translation="MKGFGYILILLSLAVACSTNSSNKNNSAETGEKWSVKMANSVMK
                     QHDSLIYYEGKEKIRWQYDVAMLAMAIDKLGVIDQKYSNYMKTYVDYFVNEDGSIKGY
                     KPEEYNIDRINPAKNLFTLYKRTGEEKYNQAIQLFIKQMENHPKTKSGGYWHKKVYPW
                     QMWLDGIYMGSPFLAQYAREYNQPQWFDVVTHQIILIHKKTLDPKTGLLYHAWDESKE
                     QRWANKNTGQSPHFWSRAMGWYVMAIVDVLDYLPEEHPDRTEIITILGKTFDALLSVA
                     DNETGLWFQVLDHGGEKGNYIEASGSCMYIYAMAKAANKGYVKKKYFQIADKKFDAIV
                     KTLIVSDNDENLVLKNVCGGCGLGGTPYRDGSYEYYITEKVVDNDSKGVGPFILAAIE
                     LNR"
     gene            1673..>1868
                     /locus_tag="PKJ43_07160"
     CDS             1673..>1868
                     /locus_tag="PKJ43_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72383.1"
                     /translation="MAAYQVKGCDFSLPIVIRKRLFPVLSRLLASEILFAQRQNFHTE
                     TISAKTQIQNNEQNNSHIPVD"
BASE COUNT          650 a          370 c          347 g          501 t
ORIGIN      
        1 ccgcaaaatt atgtctgacg gaactattga aacatcgaaa gtttctttta gataatgact
       61 aacaaaaggg gaagaggggg ctttgccctt tcttttcctt ttctttatta catacactag
      121 ctacattatt tcagttaaaa agtggcaaac gacacgttgt aacaataact gggaaaattg
      181 ttgttcccgt ttgcttataa aaagcgaatg aacgacctgt tacaacacaa taattctaaa
      241 tttagcaacc agaaaaacac ctgctgaaaa agttaaaatt ggcttacaat cagcttatat
      301 atttgacttg tagttacaat gctttaaaaa aatgttacaa gttctttaaa ataattagac
      361 tgctactcga aaccaaaaat taaataattc tgatgaaagg atttggatat atactaatac
      421 tgttatcgct ggctgttgca tgcagtacaa attcatcgaa taaaaacaac tcggctgaaa
      481 ctggcgaaaa atggtcggta aaaatggcta attcggtaat gaagcaacat gattcactta
      541 tttactacga aggaaaagaa aaaatcagat ggcaatatga tgtagccatg ttagccatgg
      601 caatcgataa gctaggtgtt attgatcaga aatattcgaa ttacatgaaa acgtatgtcg
      661 actatttcgt caacgaagat ggttccataa aaggctataa acctgaagaa tataatatag
      721 accgcattaa ccccgccaaa aaccttttca ccctttacaa acgaacgggt gaagaaaaat
      781 acaaccaggc catacaactg tttatcaaac aaatggaaaa tcatcccaaa acgaaaagcg
      841 gtggatattg gcacaagaag gtttatccct ggcagatgtg gctcgacgga atttatatgg
      901 gttcaccgtt tcttgcacaa tatgcccgcg aatacaacca acctcagtgg ttcgatgttg
      961 tgacgcatca aatcatccta atccacaaaa aaacccttga tcccaaaacc gggctactct
     1021 accatgcctg ggatgaaagc aaagaacaac gctgggcaaa caaaaatacc ggtcaatcgc
     1081 cccacttctg gagccgggcc atgggctggt acgtaatggc cattgttgac gttctcgact
     1141 acctgccaga agagcacccc gatagaacag aaatcatcac catacttgga aaaaccttcg
     1201 atgctttgct aagtgtagct gacaacgaaa ccggattatg gtttcaagtt ctcgaccatg
     1261 gtggcgaaaa aggaaattac atagaagcct ctggttcatg catgtacatc tatgcaatgg
     1321 caaaagcagc aaacaaagga tatgttaaga aaaaatactt ccaaattgcc gataaaaaat
     1381 tcgacgcaat tgtaaagacg ctgattgtct cagataatga tgaaaattta gtgctgaaaa
     1441 atgtttgtgg tggttgcgga ctcggtggaa ccccataccg cgatggaagt tacgagtatt
     1501 acataacaga aaaagtagtt gataacgaca gcaaaggcgt tggaccgttt attcttgcgg
     1561 ccattgaatt aaaccgttaa gttgtaatgt gtaaatctct ttcttaaata agaacattct
     1621 cattttatta gtaggtttat tagtcattac tcaggaaata gtgtcacagc ctttggctgc
     1681 ttaccaagtc aaaggttgtg acttttctct gccaatcgtt atccgcaaaa gacttttccc
     1741 cgtcctctca cgtctactgg catcagaaat acttttcgcc caacgccaaa attttcatac
     1801 tgaaacaatt agtgcaaaaa cacaaattca aaataatgaa caaaataatt ctcatatccc
     1861 tgttgatc
//
LOCUS       DAOPUW010000414         1867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_68055_length_1867_cov_2.760486, whole genome shotgun sequence.
ACCESSION   DAOPUW010000414 DAOPUW010000000
VERSION     DAOPUW010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68055_length_1867_cov_2.760486"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PKJ43_07165"
     CDS             <1..478
                     /locus_tag="PKJ43_07165"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNZ72384.1"
                     /translation="NRRRRCPCPVNYYIFPITKNNKDELEAQELALLEEHKIDFIVLA
                     RYMQIISDNFILHYPNRIINIHHSFLPAFVGAKPYHAAYERGVKIIGATSHYVTTELD
                     AGPIIKQDVARISHKDTVNDLIAKGRDLEKIVLSKAVSKHIDHKILVYNNRTVVFS"
     gene            490..1185
                     /locus_tag="PKJ43_07170"
     CDS             490..1185
                     /locus_tag="PKJ43_07170"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HNZ72385.1"
                     /translation="MNQAIFNTEDWNKLCRLGGTMALIVVAIIPVQIVVFGLWPPPKE
                     VLGFFELFQRNWFLGLLSLDLLYLVNNILLAFVYLALFAALRKTKAYAMFIALVLGFI
                     GIAVYYSSTSAFEMLSLSKQYFATPDAQLQQQLLAAGQAIHEGYKGTAFDVYYILNGI
                     ALVIIGFAMLNDPAKTFSKKTAVWGLISAFLMTIPSTFGIVGLIFSIASLVPWIVFSL
                     LISKQLFEISKKN"
     gene            1188..1736
                     /locus_tag="PKJ43_07175"
     CDS             1188..1736
                     /locus_tag="PKJ43_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNZ72386.1"
                     /translation="MKYLRITLVLLCIALLSVTKTEAQTFKKEIPADYGYIVKLGQTV
                     PDFDIVLPDGTKTNMQALRGKVVMLQFTASWCSVCRKEMPHIESEIWQKHKKNPSFAL
                     YGIDLKEDKEKILDFQKQIGITYPLALDLDGKIFYSFAAQDAGVTRNVIVNKSGKIVY
                     MTRLYKEEEFEEMKRVIDDLLK"
     gene            complement(1744..1815)
                     /locus_tag="PKJ43_07180"
     tRNA            complement(1744..1815)
                     /locus_tag="PKJ43_07180"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1780..1782),aa:Glu,seq:ctc)
BASE COUNT          570 a          397 c          381 g          519 t
ORIGIN      
        1 taacaggaga cgaagatgcc cctgtccagt taattattac atctttccaa ttaccaaaaa
       61 caataaagac gaacttgaag ctcaagaact tgctttgctc gaagaacaca aaatcgattt
      121 catcgtattg gctcgctaca tgcaaattat ttccgacaat tttattcttc actaccccaa
      181 tcggatcatc aacatccacc actcgttttt acctgcattt gttggggcaa aaccttacca
      241 tgcagcctac gaacgcggtg tgaaaatcat tggggcaacc agtcactacg taaccacaga
      301 gttagatgcc ggcccaatta tcaaacaaga tgttgcacga attagccaca aagacaccgt
      361 gaacgacctg attgccaaag gccgcgacct tgaaaaaatt gtgctttcaa aggcagtgtc
      421 taagcatatc gaccataaaa tattggttta caacaaccgg acagttgttt ttagttaaaa
      481 cattcaatta tgaaccaagc aattttcaac acagaggact ggaacaagct ttgtcgcttg
      541 ggtggcacaa tggcactcat tgtggtcgca attattccgg tacaaattgt cgttttcgga
      601 ttgtggccac cgcccaaaga ggtactgggg ttctttgaat tgtttcaacg aaattggttt
      661 ttggggcttc tcagcctcga tttgctctac ttggtgaaca acattttgct ggccttcgtc
      721 tacctggcac tgtttgccgc acttcgaaaa acaaaggcct acgccatgtt catcgcgttg
      781 gtgctaggtt tcattggaat tgccgtttac tattcgtcga cctcagcgtt cgaaatgctt
      841 tcgctcagca aacaatattt tgccactccc gacgctcagc ttcaacagca attgttggct
      901 gccggacaag ccattcacga gggctacaag ggaaccgctt tcgatgtata ttacatcctc
      961 aacggaattg cactggtaat tattgggttt gccatgttaa acgaccccgc gaaaacattc
     1021 agcaaaaaaa cagccgtttg gggtttgatt tcagcctttt taatgaccat cccgtcaacc
     1081 tttggaattg ttggactgat tttctccatc gcatcgctgg ttccctggat tgttttttcg
     1141 ctcttaattt caaaacaact gttcgaaatc agtaaaaaga attaacaatg aaatacttac
     1201 gaattacatt agtattgctt tgtatcgctt tattaagcgt gaccaagacc gaagcacaaa
     1261 cctttaaaaa ggaaatcccg gctgattatg gctatattgt gaagttggga caaaccgttc
     1321 ccgatttcga cattgtattg cccgacggaa caaaaaccaa tatgcaagct ttgcgaggaa
     1381 aagtagtgat gctgcaattc actgccagtt ggtgcagcgt ttgccgcaag gaaatgccgc
     1441 atatcgaaag tgaaatatgg caaaagcaca agaaaaaccc aagttttgct ctatacggta
     1501 tcgacctgaa agaagacaaa gaaaagattc ttgattttca gaagcaaatc ggaatcacat
     1561 atccacttgc acttgatttg gatggaaaaa tattttactc ttttgcagct caggatgctg
     1621 gagtgacgag aaatgttatt gtaaataaat cgggaaagat tgtttatatg acacgacttt
     1681 ataaagaaga agaatttgaa gaaatgaaac gggtcataga tgatcttctg aaataaaaag
     1741 aagtagcgcg tagcagaatc gaactgctat tttcggaatg agaatccgac ttcctaaccg
     1801 ttagaagaac gcgccagttg tgtaattcgt gtcaaaagta tagaaaatga ctttatgatg
     1861 tcatcag
//
LOCUS       DAOPUW010000415         1866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_68109_length_1866_cov_3.250690, whole genome shotgun sequence.
ACCESSION   DAOPUW010000415 DAOPUW010000000
VERSION     DAOPUW010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68109_length_1866_cov_3.250690"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PKJ43_07185"
     CDS             complement(<1..639)
                     /locus_tag="PKJ43_07185"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNZ72387.1"
                     /translation="MSFPEFINTPDTQLFLSINSNHSEFFDAFFTLYTSMLTWIPFYA
                     IILYLVFKKYNQHGFWVLLALVLTVVITDQASGLIKDLVERFRPSHEPSLLGKVHLPM
                     GPGGEFGFVSSHAANTLGFTVLVGKLTKNRNLLLVLLFWALLTAFSRIYVGVHYPMDV
                     LAGALLGGIIGWAMYKLLMFIDAHYQRKKIFYAGTWKDSEVQPAVMAMFFIVL"
     gene            complement(643..1632)
                     /gene="obgE"
                     /locus_tag="PKJ43_07190"
     CDS             complement(643..1632)
                     /gene="obgE"
                     /locus_tag="PKJ43_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNZ72388.1"
                     /translation="MAETNFVDFVRIYCRSGNGGPGSTHFRREKFEPKGGPDGGDGGR
                     GGHIILRGNAQMWTLLHLKYRKHIYAGHGESGGRQCSTGADGEDIIIDVPLGTVVRDS
                     ETEEFIFEITQDGEEKILMQGGRGGLGNTNFKSATNQAPRFAQPGEPFEDGWCILELK
                     ILADVGLVGFPSVGKSTLLSVVSAAKPKIADYPFTTLVPNLGIVSYRDNQSFVMADIP
                     GIIEGAHEGRGLGLRFLRHIERNSMLLFMVPADSKNHREEYNVLLNELEMYNPELLDK
                     QRLLAICKADFLDEELMQEISREFSDVPHIFISSVTGFNIQRLKDRIWQILNS"
BASE COUNT          585 a          446 c          338 g          497 t
ORIGIN      
        1 gagaacaata aaaaacatgg ccataaccgc tggttgaacc tctgagtctt tccatgttcc
       61 ggcataaaaa atctttttac gctgataatg agcatcgata aacatcaaaa gcttatacat
      121 tgcccatccg ataatacctc ccaagagcgc accagcaaga acatccattg gataatgtac
      181 cccaacatat atgcgagaaa aagcagttag caaggcccaa aataacaata caagcaaaag
      241 gttccggttt ttggtcagtt taccaacaag aacagtaaaa cccaaagtat tggccgcatg
      301 cgacgaaaca aagccaaact caccacccgg gcccattggc aaatgaactt tccctaataa
      361 tgaaggttca tgcgaaggcc tgaagcgttc aaccagatct tttataagtc ccgaagcctg
      421 atcggttatc accacggtaa gtacaagagc caaaagcacc caaaaaccat gctgattata
      481 ttttttgaaa acaagatata atataatggc atagaatggt atccatgtca gcatactagt
      541 atagagcgta aaaaacgcat cgaaaaattc agaatggtta ctgttgatcg acagaaacaa
      601 ttgggtatcg ggtgtattta taaattcggg aaagctcatg ttttaactat tgagtatctg
      661 ccaaatccgg tcttttaatc gttgaatatt aaaaccagtc accgaagata taaaaatatg
      721 tggtacatct gaaaactcac gactaatttc ctgcatcagc tcctcatcga gaaaatcggc
      781 tttacaaatt gccaaaagcc tctgcttatc aagcagttct ggattataca tttcaagttc
      841 gttcaacaaa acattatact cttcacgatg gtttttacta tcggcaggca ccatgaacaa
      901 taacattgaa tttcgttcaa tgtgccgcaa aaagcgcaaa cccaggccac gaccttcgtg
      961 cgctccttca ataatcccgg ggatgtcggc cattacaaac gactgattgt cgcgataact
     1021 aacaatacct aaattgggga caagagtagt aaaaggataa tcggcaattt taggttttgc
     1081 cgcagaaact accgataaaa gagttgattt tccgacactt gggaaaccta caagcccaac
     1141 atcggccagt atttttaatt caagaatgca ccatccgtcc tcgaagggct cgcccggttg
     1201 ggcaaaccgg ggtgcttggt ttgtggcaga tttaaagttt gtatttccca atcctccgcg
     1261 tccaccttgc attaatattt tttcttcacc atcctgggta atttcaaaaa tgaactcttc
     1321 agtttcagag tcacgcacta ctgtacccag tggaacatca atgattatat cttcgccatc
     1381 ggcacctgta ctacactgac ggccacccga ttcgccgtgt ccggcataaa tatgttttcg
     1441 gtatttcaaa tggagcaaag tccacatctg agcatttcca cgtaaaataa tgtggcctcc
     1501 ccggcctcca tctcccccat cgggtcctcc cttaggctca aacttttctc gacgaaaatg
     1561 tgtcgaaccc gggcctccgt tacccgaacg acaataaatc cttacaaaat caacaaaatt
     1621 cgtctctgcc atacacttat attatttgcc acaaaggtaa cataaccgat gaaacaaaaa
     1681 aaagccctgc ctaatggcag agcctttccg aaaattcatc atctccttat tctgattttt
     1741 tcgatgcaca acatggtttt tgttcgccag tacaagcttt tgattccgat ttttgggctt
     1801 cagaacatcc tgctttttta gcagcttcag ggcaagaagt tttttcttta gcagcagcac
     1861 tatcag
//
LOCUS       DAOPUW010000416         1861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_68415_length_1861_cov_2.347176, whole genome shotgun sequence.
ACCESSION   DAOPUW010000416 DAOPUW010000000
VERSION     DAOPUW010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68415_length_1861_cov_2.347176"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..871)
                     /gene="rlmB"
                     /locus_tag="PKJ43_07195"
     CDS             complement(116..871)
                     /gene="rlmB"
                     /locus_tag="PKJ43_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNZ72389.1"
                     /translation="MNNNDFIFGIRPIIEAIRSGKEIEKILIKKGLKGELFIELNELI
                     QENNLHYQLVPEEKLNRLTRKNHQGVVAFISPVPFYDAEEIITRTYENGETPFFVYLD
                     QVSDVRNFGAIVRNAECAGVHAILVPEKGSAQIGADAVQTSAGALHIVPVCKIKNPVK
                     ALETIKKNGVKIIAATEKADIDYTEAQMDGPCLLVMGAEDQGISPEILRIADEYVRIP
                     VFGKIESLNVAVASGILMYEVVRKRKEENKSTN"
     gene            complement(881..>1861)
                     /locus_tag="PKJ43_07200"
     CDS             complement(881..>1861)
                     /locus_tag="PKJ43_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ72390.1"
                     /translation="RSGKIVAEIKGLSKAYGTLKVLENIDLYIEQGEKIAFVGKNGEG
                     KTTLARIILSELDYTGTCKIGHNVKIGYFAQNQADLLDGELTVLETIDKVAVGEIRTK
                     IRDILGAFMFSGDDVEKKVKVLSGGERTRLAMVRLMLEPVNFLVLDEPTNHLDMLSKE
                     ILKEALRDFTGTVLVVSHDREFLDGLVDCVFEFKDKKIKQHLGGIYDFINKKKIETLQ
                     ELNKKSPSEQASRTSVIEEKDAEISFEEKKEINRQINKQEKLIETIEKKIHSLENKIE
                     ELNQKLSATGGSDFLLFGEYEQLKKQHETAMSQWEEAQTTLDAWTSKKIW"
BASE COUNT          423 a          420 c          378 g          640 t
ORIGIN      
        1 cgcaataacg agcaccttaa gcagatacta agtgagaaaa ttcagaaaat ttcgggcata
       61 acaactaccg aaacttttat ctcactggaa gaaacattta acagagaagt gcctattaat
      121 tagtgctttt gttttcttcc ttgcgttttc gtaccacttc atacattaat attcctgacg
      181 ctactgcaac gttcagcgat tcgattttac cgaatacggg tattcttacg tattcgtcgg
      241 caattcgtaa tatttcagga gagatgcctt gatcttcagc tcccataact agcaagcaag
      301 ggccatccat ttgagcctcg gtataatcga tgtcagcttt ttcggttgct gcgattattt
      361 tcaccccgtt ctttttgata gtttccaagg cttttaccgg gtttttaatt ttgcaaacgg
      421 gtacaatgtg cagcgcacct gctgaggttt gcacggcatc ggcaccaatc tgggctgacc
      481 ccttttctgg aaccagtatg gcatgaacac ctgcacattc ggcattgcga acaatggctc
      541 caaaattgcg cacgtcagat acttggtcga gataaacaaa aaacggagtt tccccatttt
      601 cgtaggtgcg ggttattatt tcttcggcat cgtagaacgg aactggcgag atgaatgcca
      661 caaccccttg atggtttttc cgggttagcc ggtttagttt ttcctccgga acaagttggt
      721 aatgaaggtt attttcctgg atgagctcgt tgagttcgat gaaaagctct ccttttaatc
      781 ccttctttat caatattttc tctatctctt tgcccgatcg gatggcctca ataatggggc
      841 gtatgccgaa tataaaatcg ttgttattca tttgataaaa ttaccagatt tttttcgatg
      901 tccaagcgtc gagggttgtt tgtgcctctt cccattggct cattgctgtt tcgtgttgtt
      961 ttttaagctg ttcatactcg ccgaacaaca agaaatcgct tcctcccgta gccgaaagtt
     1021 tttgattgag ttcttctatt ttattttcca gcgaatgtat ttttttttcg atggtttcga
     1081 taagtttctc ctgcttgttt atttgccggt ttatttcctt tttctcttca aaagatattt
     1141 cagcatcctt ttcttcaata acagatgttc tgctggcttg ttccgaaggg ctttttttgt
     1201 tcagttcttg cagggtttct attttctttt tattgataaa atcgtaaata cccccaaggt
     1261 gctgttttat ttttttgtct ttgaattcga acacgcaatc taccagcccg tcgagaaatt
     1321 ccctgtcgtg cgataccact agtacagtac ctgtaaagtc gcgaagggct tctttcagta
     1381 tttctttcga aagcatatcg aggtggttgg tgggctcgtc gagaacgagg aaatttaccg
     1441 gttccagcat taagcgcacc atggccagcc ttgttcgttc gccacccgag agaactttta
     1501 ctttcttctc tacatcgtcg cccgagaaca tgaatgcccc cagtatgtcg cggatttttg
     1561 tgcgtatttc gccaacagca actttatcaa tagtttccaa cacggtgagc tcgccatcga
     1621 gcaggtcggc ttggttctgg gcaaaatagc caattttcac attgtgtcca attttacatg
     1681 ttccggtgta atccagttcg ctgagtatta ttcgggccag ggtggttttt ccttccccgt
     1741 ttttccccac aaaggctatt ttttctccct gttcaatata aaggtcgatg ttttcgagca
     1801 ctttcagtgt cccgtatgct ttgctgagac ctttaatttc ggcaacgatt ttacccgatc
     1861 t
//
LOCUS       DAOPUW010000417         1858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_68577_length_1858_cov_2.982806, whole genome shotgun sequence.
ACCESSION   DAOPUW010000417 DAOPUW010000000
VERSION     DAOPUW010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68577_length_1858_cov_2.982806"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PKJ43_07205"
     CDS             <1..420
                     /locus_tag="PKJ43_07205"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNZ72391.1"
                     /translation="HGVDYAAPTGTPVYSIGDGVVVKKTYQAGGGGNYLSIKHNSVYT
                     SQYMHLSKFAKGIAAGSRVKQGQLIGYVGMTGLASGPHLDFRIYYNGSPVDPLKVETP
                     PVEPISEANKASFVVVRDSLLKVLDTVKYPEVLLSKR"
     gene            complement(409..972)
                     /gene="purN"
                     /locus_tag="PKJ43_07210"
     CDS             complement(409..972)
                     /gene="purN"
                     /locus_tag="PKJ43_07210"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNZ72392.1"
                     /translation="MKKIAIFASGSGSNAENIARFFKERENLNIECIYSNKNDAYVLQ
                     RAHNLLIPSKVFTKNDFYFSDIILNDLKNREVDLIVLAGFLWLVPQNLIDNFPIINIH
                     PALLPKYGGKGMYGDNVHKAVVANGEKESGITIHYVNDKYDDGQIIFQAKCEVLPTDT
                     ADDVAKKVHQLEYRYFPEIIEKVVLSL"
     gene            1143..1379
                     /locus_tag="PKJ43_07215"
     CDS             1143..1379
                     /locus_tag="PKJ43_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNZ72393.1"
                     /translation="MSDIASKVKAIIVDKLGVDESEVTPEASFTNDLGADSLDTVELI
                     MEFEKEFDMAIPDDQAEKISTVGDAIQYIENNTK"
     gene            1398..>1858
                     /locus_tag="PKJ43_07220"
     CDS             1398..>1858
                     /locus_tag="PKJ43_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNZ72394.1"
                     /translation="MELKRVVVTGLGTINPIGNNIEEYWNNLINGVSGAAPITRFDAT
                     FHKTRFACEVKNWDPLVHFDRKDARKYDLFTQFALVSAKQAILDSQIDLDSIDKNRVG
                     VIWGAGIGGIQTFEEEVMGFNTENPKTSPFFIPKMIANMASGCISIEYGFRG"
BASE COUNT          553 a          332 c          390 g          583 t
ORIGIN      
        1 cacggagttg attatgccgc accaacaggg actcccgttt attccattgg tgatggggta
       61 gtggtaaaaa agacatatca agcagggggt ggtggcaact atctttctat taaacacaat
      121 tctgtgtaca cttcgcaata tatgcacctg agcaaatttg ccaaaggaat tgctgccggt
      181 tctagggtga aacaaggtca acttattggt tatgtaggaa tgacgggctt ggcctcgggg
      241 ccgcatctcg atttcagaat ttattataat ggttcccccg ttgatccgtt gaaggtagaa
      301 acccctcctg ttgagccgat tagcgaggca aacaaagcaa gttttgttgt cgttcgcgat
      361 tcgttattga aagtactcga caccgtgaag tatcctgaag ttttgctttc aaagcgatag
      421 gacaactttt tcaattattt cagggaaata ccggtattct agctggtgta ctttttttgc
      481 cacatcgtcg gcagtatcgg tgggcagtac ttcacatttt gcctgaaaga tgatctgtcc
      541 gtcatcatat ttatcgttca cgtaatgaat ggtaattccc gattcttttt caccatttgc
      601 cacaaccgct ttgtgaacgt tatctccgta catccctttt cctccatatt taggtagaag
      661 ggccgggtgg atgttgatga ttggaaaatt gtcgatcagg ttttggggaa cgagccaaag
      721 aaaaccagct aatactatta aatcaacttc tctgtttttc aagtcgttaa ggatgatatc
      781 agaaaaatag aaatcatttt tagtgaatac tttactggga atcaataggt tgtgtgctct
      841 ttgtaataca taagcatcat ttttgttcga atatatgcat tcgatgttca aattttctct
      901 ctctttaaaa aaacgggcga tgttctccgc gtttgaaccc gaacctgaag caaaaatggc
      961 tatttttttc atttttagta gtttatattt ttaaagacca tttcaataat ttcggcaaaa
     1021 atacatctta agattaatct tgtatgctca acaagcataa aaatttggat ttgtaaatcg
     1081 tttctttatt actttgcagc gatttaaaaa tagcgaatac gtttaactaa aaaattgaaa
     1141 ttatgtctga cattgcatca aaagttaagg caattatcgt tgacaaactg ggtgttgacg
     1201 aaagtgaagt tacacccgaa gcaagcttta ccaatgactt gggagcagat tcactcgata
     1261 cagttgagtt gatcatggaa ttcgaaaaag aattcgatat ggcaatacct gatgatcagg
     1321 ctgaaaaaat cagcacagta ggtgatgcca ttcaatacat cgaaaataac accaagtaat
     1381 aaacatttta ttattctatg gaattaaaac gagtagttgt aactggtctt ggtaccatta
     1441 accctattgg gaacaatatt gaagaatatt ggaataatct gatcaatgga gtaagtggtg
     1501 ccgcacctat tactcgtttt gatgcaactt ttcacaaaac aaggtttgcc tgcgaggtga
     1561 aaaactggga tccgttagtt cacttcgaca ggaaagatgc ccggaagtac gacttattta
     1621 cgcaatttgc gttagtgtca gccaagcagg caattcttga ttcacaaatt gatctcgact
     1681 caattgataa aaatcgtgta ggtgttatct ggggggcagg tattggtgga atccagactt
     1741 ttgaagaaga agtaatgggt tttaataccg aaaatccgaa gacatcgcct tttttcattc
     1801 ccaagatgat tgccaatatg gccagtggtt gcatttcaat cgagtatggt tttcgtgg
//
LOCUS       DAOPUW010000418         1857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_68624_length_1857_cov_3.150943, whole genome shotgun sequence.
ACCESSION   DAOPUW010000418 DAOPUW010000000
VERSION     DAOPUW010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68624_length_1857_cov_3.150943"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PKJ43_07225"
     CDS             complement(<1..388)
                     /locus_tag="PKJ43_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNZ72395.1"
                     /translation="MYKVFFNDRIIYCGAGSKNSGIVNSLSFYVTDSKEAGNAWQIFL
                     GDSMLRDMYFLSESDDIAWGFFHSLFQQIDAAGGLVLNSEEKLLCIKRWGKWDLPKGK
                     MEKGEEPAQTALREVEEECGIFPLIEH"
     gene            414..1046
                     /gene="pyrE"
                     /locus_tag="PKJ43_07230"
     CDS             414..1046
                     /gene="pyrE"
                     /locus_tag="PKJ43_07230"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNZ72396.1"
                     /translation="MNDYQLEVTKKLLEINTIKIQPSSPFTWASGWLSPIYCDNRKVL
                     SYPATRTFICDQFVRIIKEKYPQAEIIAGVATGAIAHGVLVAEKLGLPFVYVRSKPKD
                     HGLENLIEGDLQVGQKVVIIEDLVSTGGSSLKAAEALSNFGGDVLGMVAIFTYDFPVA
                     NENFKKAGVELSTLSNYQTLIKLAFDEGEVTLEEMENLQNWRKNPAEWGR"
     gene            1114..1563
                     /locus_tag="PKJ43_07235"
     CDS             1114..1563
                     /locus_tag="PKJ43_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72397.1"
                     /translation="MGTSKFVSDVKIIKHNNVVVYNFLSDFNNLRILFNEYTLAQLSK
                     QLPNMEITNCVCDADSCLFTLSNNSQGGVKIIEREHPKTIKLTGEGKLPFEMFFWIQL
                     LPTSAYETKIRLTLHAELNMMLKMLAGKKLKDGLDKIADALTMLPYH"
     gene            complement(1567..>1857)
                     /locus_tag="PKJ43_07240"
     CDS             complement(1567..>1857)
                     /locus_tag="PKJ43_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72398.1"
                     /translation="SLALEVDREFIIDEVVLLMIDLINKWYQQLNSGNLQYIDEEYIS
                     KLYRFGVTSVFSDCEGQFTGKITGINPYGHLIVLKESGELNSYAMKEISFIA"
BASE COUNT          630 a          393 c          328 g          506 t
ORIGIN      
        1 catgttcgat tagcggaaaa atcccacatt cttcctcaac ttcacgcaaa gccgtttgag
       61 cgggttcttc tcctttctcc atttttcctt taggcaaatc ccatttaccc caccgtttaa
      121 tgcacaacaa cttttcttca gaatttaaca caagtccacc ggcagcatca atttgctgaa
      181 aaagactgtg gaaaaatccc caggctatgt catcgctttc agacaagaaa tacatgtcgc
      241 gcaacataga gtcgccaagg aaaatttgcc atgcattgcc agcttcttta gagtcagtca
      301 cataaaaaga aagactatta actattccag aatttttgga accggcacca caatatatga
      361 tccggtcatt aaaaaaaact ttgtacattt ggccaaaatt aaaaggaaaa acaatgaacg
      421 attatcagct tgaagtaaca aaaaaacttt tagaaattaa tacaataaaa atacaacctt
      481 catcgccttt cacatgggca tcgggttggc tttcgcctat atattgcgac aaccgtaaag
      541 tgctgtcgta cccagccaca agaacattta tttgcgacca atttgtccgc atcataaaag
      601 aaaaataccc tcaggccgag attattgccg gtgttgctac cggagccata gctcatgggg
      661 tgttagtggc cgagaaactt ggattgccct ttgtttacgt tcgcagcaaa cccaaagatc
      721 atggactgga aaacctcatt gaaggtgatt tacaggttgg acaaaaagtt gtaatcattg
      781 aagatcttgt ttctacagga ggaagcagtc taaaagctgc tgaagcattg agcaattttg
      841 gcggtgatgt tctgggtatg gttgctattt tcacctacga ttttcccgtg gcaaatgaaa
      901 acttcaaaaa agcaggagtt gagttaagca cgcttagtaa ctatcaaacc cttataaaat
      961 tagcattcga tgagggcgaa gtaacccttg aagaaatgga aaaccttcaa aactggagaa
     1021 aaaacccggc tgaatggggc agataataat catttgcaaa ttataacatt agccggatca
     1081 cacgataccg gctttttcat agatatataa aaaatgggca caagtaaatt tgttagcgac
     1141 gttaaaataa ttaaacacaa caatgttgtg gtgtacaatt ttttaagcga ctttaataac
     1201 cttcgtatac tgttcaatga atataccttg gcgcaattat cgaaacaatt gccaaacatg
     1261 gaaataacca attgtgtatg cgacgctgac agttgtttgt tcaccctctc gaataacagt
     1321 caggggggcg tgaagataat cgaacgcgaa catcctaaaa ccataaaact aaccggcgaa
     1381 ggtaagctgc ctttcgaaat gttcttttgg attcaactat taccaacatc ggcttacgaa
     1441 acaaaaatca ggttaactct tcatgctgaa cttaacatga tgctcaaaat gctggcaggg
     1501 aaaaaattaa aagacggctt agacaaaatt gccgatgctc tcactatgct tccttatcac
     1561 taacaattat gcaataaatg agatttcttt catagcatat gaattcaact caccactttc
     1621 ttttaaaaca ataagatgcc cataagggtt aatcccggta atttttccgg taaattgccc
     1681 ttcacaatca gaaaaaacag acgtaacccc aaaccgataa agtttgctaa tgtattcttc
     1741 atcaatatac tgcaaattgc cagaatttaa ttgttgatac catttattta tcaaatcaat
     1801 catcaacagc acaacctcat cgataataaa ctcccgatca acctcgagcg ccagcga
//
LOCUS       DAOPUW010000419         1857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_68644_length_1857_cov_2.251387, whole genome shotgun sequence.
ACCESSION   DAOPUW010000419 DAOPUW010000000
VERSION     DAOPUW010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68644_length_1857_cov_2.251387"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..1552)
                     /locus_tag="PKJ43_07245"
     CDS             complement(266..1552)
                     /locus_tag="PKJ43_07245"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNZ72399.1"
                     /translation="MHEDIIHFIWKHKPALFNGLTCQPGGKVEVISPGNHNFDSGPDF
                     FNAKVKIDNTIWAGNVEIHVKSSDWNRHGHNKDEAYNNVILHVVAVNDMPVSNANGDP
                     VTTLEIPYPHDLEWELQRLVATESWIPCAKDIGKVNPISIQMWLTSLCIQRLEQKTDQ
                     VQNLVTEYNGSWEEAFYVSLARSFGLKINSLPFELLAKSTPLKALSKVKDNLISVEAL
                     LFGQAGLLNFSAITADDYMQELVKEYNYQQRKFQLIPIPGHLWKFMRLRPNAFPTIRL
                     SQFARLIHNSAGLFSQCMEAGTLSDYVEILRVISSSYWNNHYTFGKVTEGKKKLLGEN
                     AIKTIILNTIVPFMFTYGKVRGNEVLKDKSLNLLEELPPESNSIVKGFESIGIKADNS
                     FFSQALVQLKNQYCDKRKCLFCQIGTSVLLKKVEKT"
     gene            1662..1739
                     /locus_tag="PKJ43_07250"
     tRNA            1662..1739
                     /locus_tag="PKJ43_07250"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1697..1699,aa:Pro,seq:cgg)
BASE COUNT          474 a          392 c          395 g          596 t
ORIGIN      
        1 cgtactcttt aaagatctcc agcttctgtt ctgcggtcaa ataattcata gctactcaat
       61 atattagttg ggtatatggt atgctttttt ttcagactgc aaaagtaata gtttttttaa
      121 tgccagagta cttttactac ctatttttta aagccatttt caaagagtaa acaatctcgt
      181 tgtctatatg tcagatattc aataaagtat aacaatcaat tgtgcgattt aattattttt
      241 ttgttgtgaa atgtaggtaa tatgactacg ttttttcgac ttttttgagc agtacactgg
      301 taccaatttg gcaaaacagg catttccttt tatcgcagta ctggttttta agctgtacca
      361 atgcctgaga aaagaatgag ttgtctgcct ttatcccaat ggattcgaac ccttttacga
      421 ttgaattgga ttcgggcggc agttcttcca gcaggttgag cgatttatct ttgagaactt
      481 cgttacctct gaccttgccg taagtaaaca tgaatggcac tatggtgttc aaaattattg
      541 tcttaatcgc attctcaccc aacaacttct ttttgccttc agtcactttg ccgaatgtgt
      601 agtgattatt ccaataggag gaagagataa ccctcaaaat ttccacataa tcggatagcg
      661 tgcccgcctc catgcattgc gaaaaaaggc cggcagaatt gtgtattaac cgggcaaatt
      721 gggataaacg aattgtggga aaagcgtttg gcctgagccg cataaatttc cacaggtgac
      781 cgggtatggg aattagttga aatttccttt gttgataatt gtactccttg accaactcct
      841 gcatgtagtc atccgcggtg atagccgaaa agttgagcaa tcctgcttgg ccaaaaagca
      901 gcgcttcaac gctaataaga ttatccttta ctttcgacag tgccttcagg ggtgtcgatt
      961 ttgccaacag ttcaaatggc aaactattga tctttagccc aaaactacgt gccaatgata
     1021 cgtaaaaggc ctcttcccaa ctgccattat actctgtaac aaggttttga acttgatccg
     1081 tcttttgctc gagccgctga atacacagtg aggtcaacca catttggatg ctgatggggt
     1141 ttaccttacc tatatctttg gcacacggga tccaactttc tgtggctaca agccgttgta
     1201 gttcccactc taagtcgtgg ggatacggta tttcgagggt agttaccgga tcgccgttgg
     1261 cattgcttac gggcatatcg tttactgcca caacgtgcag aatgacattg ttgtacgcct
     1321 catccttgtt gtgcccgtgc ctgttccagt ccgatgattt aacatgaatt tccacgttac
     1381 ctgcccaaat agtattgtca atcttaacct ttgcattgaa aaaatcgggg cctgagtcga
     1441 aattgtgatt gccgggtgag ataacttcaa cttttccccc gggctggcat gtgagcccgt
     1501 tgaataaagc gggcttgtgt ttccagataa agtggataat gtcctcgtgc atgtgattgg
     1561 cattttattc gaaggtagag aaaaattcgt ccactgcgaa gtggtaagtg gattcttttt
     1621 ataattttgc actcgttatt tttggactta accaaaaatt tcggggtgta gcgcagcccg
     1681 gttagcgcgc gtcgttcggg acgacgaggt cgtgagttcg aatctcacca ccccgaccat
     1741 attccttctt aattccccta cttttttctc atcctcaggt agatattggt taatactttc
     1801 tttgtgtaca gagggaattc accattcatt acccacgagt agtagctggg ctcggtg
//
LOCUS       DAOPUW010000420         1856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_68690_length_1856_cov_3.198778, whole genome shotgun sequence.
ACCESSION   DAOPUW010000420 DAOPUW010000000
VERSION     DAOPUW010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68690_length_1856_cov_3.198778"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1856)
                     /locus_tag="PKJ43_07255"
     CDS             complement(<1..>1856)
                     /locus_tag="PKJ43_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ72400.1"
                     /translation="VKNRIDDWSGAVFCIAEDEHFVWCGTWGGGIKKVDKSTLEYVSI
                     NFDDKDQHRNSIFDIEIHDSFLWIANVGIGLIKYDPVSGTQKVYTHGPEYPCFPEERI
                     IDINFQNDTTCWLCTDGAGLYRFNPKTEKIVNITENQPLTGNVIQSVIPDSLGNIWVA
                     SISGVSSYNPITNVCHNFDKSNGLVNNQLNKSALFLDTIDHIIYTGSVEGVNYFNPYS
                     TRIDSSSQKVIITDFRINGKQVFPDKKHSKKPIDIAGEVHLYPKDRIITIHFSSLEFN
                     PSQKNKYYYRLEGFENEWNETAFSKNFVQYTNLYPGKYQFKVKSANNDGVKSSEYTKV
                     TIIVHPAFWQTIWFVLLVLLLIASMVVLYIRWRHKSLLLAKIVLEKKVAARTAEIQQQ
                     KERIEQQNHLLEQANQTKDKFFSIIGHDLRNPVTTIDQLIGLVISRFDEDSRDKTMNY
                     LLFLKKASAGTLELLDDLLMWARTQTNRIEIHKEQIPVQELLDSLLNVCMPVAENKNI
                     ELLFPDSVDWVVFGDRNTLLTSLRNMVTNAIKFSYPSSRVIVSIEEKPDTVIFNITDS
                     GIGMNEKEIARLFKIENIHSKQGTSGETGSGLGLVLCKEFLALNGGEIWVKS"
BASE COUNT          551 a          383 c          331 g          591 t
ORIGIN      
        1 gcttttaacc caaatctcac ctccgttcag tgccaaaaat tctttacata aaaccaaccc
       61 aagaccactt ccggtttcgc ctgaagttcc ttgtttagaa tgaatatttt caattttgaa
      121 caatcttgct atttcttttt cattcatccc aatccccgaa tcggtgatgt tgaaaattac
      181 agtatctggt ttttcttcta tggaaacaat taccctgctt gatggataag aaaactttat
      241 tgcattggta accatgttcc taagcgaagt caacaacgtg ttcctatcgc caaatactac
      301 ccaatcgacc gaatcgggaa acaacaattc aatattctta ttctcggcaa caggcataca
      361 aacatttaat aacgaatcaa gtaattcctg aacaggaatt tgttccttat gaatttcaat
      421 tcggttggtt tgtgttcgtg cccacataag taagtcgtcg agcagctcga gtgtccccgc
      481 cgaagctttt ttcaagaaca ggagataatt catcgtttta tctcttgaat cttcatcgaa
      541 acgactaata actaaaccga taagctggtc aatagtcgtc actgggttcc gaagatcgtg
      601 gccaatgatg gaaaagaatt tatctttggt ttgattcgct tgttccagca aatgattctg
      661 ttgctctatt cgttcttttt gctgctgaat ttcagccgtt cttgctgcca cttttttctc
      721 gagaacgatc tttgcaagta acaatgactt atgacgccat cttatgtaaa gcacaaccat
      781 tgaggcgatt aaaagtaaaa ctaaaagaac aaaccaaatt gtttgccaaa atgccggatg
      841 aacaattatg gtgactttag tatattctga agatttaact ccatcgttat tagcactttt
      901 aactttaaac tgatacttcc ccggataaag gttggtgtat tgtacaaagt tttttgaaaa
      961 tgcggtttcg ttccattcat tctcgaaacc ttcgagccgg taatagtatt tattcttttg
     1021 agaggggtta aactcgagcg aagaaaaatg aatggttata atccggtctt tcggatatag
     1081 atgaacttca ccggcaatat caataggttt tttgctgtgc tttttatcgg gaaaaacttg
     1141 cttcccatta atccgaaaat cggtaatgat aactttctgc gaggaagaat cgatccgggt
     1201 agaatatggg ttaaaatagt taaccccttc aacacttccg gtatagataa tgtggtcgat
     1261 ggtgtcgaga aacaatgccg atttattcaa ctgattgttt actagtccat tacttttatc
     1321 gaaattatgg caaacatttg taatcgggtt ataactcgaa acacccgaaa tagacgcgac
     1381 ccaaatattt cccaaagaat cgggaataac tgattgtata acattacccg taagcggttg
     1441 attttccgtg atattaacta ttttttcggt tttggggttg aaccggtata atccggcacc
     1501 atcagtacaa agccagcaag ttgtatcgtt ttgaaaattg atatcgatga tacgctcttc
     1561 agggaaacat ggatattcgg gcccatgcgt atacaccttt tgagtgcctg aaacagggtc
     1621 gtattttatc aggccgatac ctacattggc tatccacaaa aacgaatcat gtatttcaat
     1681 gtcaaaaata ctgttgcgat gttggtcctt gtcgtcaaaa ttaatcgaca catattcaag
     1741 agtgctcttg tctacttttt taatcccacc tccccaagtt ccacaccaaa caaaatgctc
     1801 atcttcagca atacaaaaaa cagcaccgct ccagtcgtcg attcgatttt ttactt
//
LOCUS       DAOPUW010000421         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_68763_length_1855_cov_2.602222, whole genome shotgun sequence.
ACCESSION   DAOPUW010000421 DAOPUW010000000
VERSION     DAOPUW010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68763_length_1855_cov_2.602222"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1855
                     /locus_tag="PKJ43_07260"
     CDS             <1..>1855
                     /locus_tag="PKJ43_07260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNZ72401.1"
                     /translation="AKFLQLFIDDNFLLRLKERLPKPLKKLSFEQTEADIYFDKKDQF
                     FHSHCFIVDSGCIAKYSDYLAELSVFNHIIFVIRNEQELLENIDNLKIFHWLKYHFTP
                     DELQLLLERSDLNNEENIVSLTTENTIFEAHLSLPTQKSTLHNPLFVINHQGKYLLFN
                     KYFEDSFINHQNSNNIPLSNGDIWDLQTSAQLEEQDRIILSSGKGIINQVVELTQKSG
                     QTQKCIISKMPLKDGNGKTWFITGAITTKESSHEDEEKLFPDWKLLQILMDNIPDSIY
                     FKDLESRFIRINRAQAKLIGIESPEEAIGKTDFDFFNIELAKKTMADEQRIINTSIPI
                     NGVEFVGTKDGRFRWVSTSKVPITDKNGKVIGTVGITHDIDKMMRVEQRLKSERDMLQ
                     LLIDHIPSPIYFKDTQSKFTRVNLAQAKLLGANSPEDVIGKSDFDYYTPENADEFYND
                     EKNIIDKHKPLINKVEECFHPGDGLHWFSTTKIPLKNEDGELSGIVGVSHDITDQILI
                     KKNLELAKEKAEIANSAKSNFLSNMSHEIRTPMNGVIGMAEVLNMTDLDEEQKRIVDL
                     IIRSGNNLLNIINDILDLSKIESEKLIIEAVPIDIKSIVREVHEMFYFGAIE"
BASE COUNT          665 a          358 c          347 g          485 t
ORIGIN      
        1 gcaaaatttt tacagctttt tatcgatgat aactttcttt tgaggctgaa agagcgactc
       61 ccaaaaccac taaaaaaatt atcttttgaa caaacagagg ctgatattta ttttgacaag
      121 aaagaccaat tctttcacag ccattgcttt atagtcgaca gcggttgtat tgcaaagtat
      181 agtgactatc ttgctgaact ttcagtcttt aatcacatca tttttgttat acggaatgaa
      241 caggaattgt tagaaaacat tgacaattta aagattttcc attggctaaa atatcatttt
      301 acaccggatg aattacagct tttactcgag agatcggatt tgaataacga ggaaaatata
      361 gtgtcattaa caactgagaa tacaattttc gaagcacatt tgtcacttcc tacacaaaag
      421 tctactttac acaatccgct cttcgtcata aaccatcaag ggaaatactt gctattcaat
      481 aaatattttg aagatagttt cattaaccat caaaacagca ataatatacc attaagcaat
      541 ggagatatat gggatctgca aacatcagcc cagctcgagg aacaagaccg tataattcta
      601 tcatcgggaa aaggaattat taatcaagtt gttgaattga cacaaaaatc ggggcaaacc
      661 caaaaatgta tcatcagcaa aatgccgtta aaagatggta acggtaaaac atggttcatt
      721 acaggagcaa ttaccacaaa agaatcgagc catgaagatg aagaaaaact tttccccgat
      781 tggaaacttt tacagatttt gatggacaat atccctgatt ctatctattt caaagacctc
      841 gaaagccgtt tcatcaggat aaaccgggca caggcaaaac tcatcggaat tgaatctccg
      901 gaagaagcca ttggcaaaac cgattttgac tttttcaaca ttgaactggc aaagaaaacc
      961 atggctgacg agcaacggat aattaacact tcaattccaa taaatggggt tgaatttgta
     1021 ggaaccaaag atgggcgttt ccgctgggta agtacatcga aggtgccaat taccgacaaa
     1081 aacgggaaag taatcggaac cgttggaatt acgcacgaca tagacaaaat gatgagggtt
     1141 gaacaacgct tgaaatcaga acgcgatatg ttacaattac ttatcgacca tattccctcg
     1201 cccatttatt tcaaagacac ccaatcgaaa tttacccgcg taaaccttgc ccaggcaaaa
     1261 ctccttggtg ccaattctcc cgaagatgtg attggaaaat cagatttcga ctattacacc
     1321 cctgagaatg ccgacgaatt ttataacgac gaaaaaaata tcattgataa acacaaaccc
     1381 ctaattaaca aggtagaaga atgttttcac ccaggggatg ggctccattg gttttctact
     1441 acaaaaatac cgctaaaaaa tgaagatggc gaactgtcgg gcatagttgg cgtatcgcac
     1501 gacattaccg atcagatact tataaaaaag aaccttgaac tggccaaaga aaaagctgaa
     1561 atagcaaatt cggcaaaaag taattttctg tcgaatatgt cgcacgaaat tcgtacgcca
     1621 atgaacgggg taatcggcat ggctgaagtg cttaatatga ccgatctgga cgaagaacag
     1681 aagcgcattg tggacctgat tatcagatcg ggaaacaatt tattgaacat tatcaatgac
     1741 atacttgacc tctcgaaaat tgaaagtgag aaactaatca tcgaagcagt cccaatcgac
     1801 ataaaatcaa tcgtcaggga ggtacatgaa atgttctatt ttggggctat tgaga
//
LOCUS       DAOPUW010000422         1852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_68949_length_1852_cov_2.079021, whole genome shotgun sequence.
ACCESSION   DAOPUW010000422 DAOPUW010000000
VERSION     DAOPUW010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68949_length_1852_cov_2.079021"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..1668)
                     /locus_tag="PKJ43_07265"
     CDS             complement(262..1668)
                     /locus_tag="PKJ43_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ72402.1"
                     /translation="MKSMMATSEVSLKEKIGYGLGDAASSMFWKIFSFYLAIFYTDIF
                     GISAVAAGTMFLVTRIWDTANDPIMGIIGDRTNTRWGKFRPYLLWGAIPFGILGILLF
                     TTPNFDTTGRLVYAYITYTFMMMAYTAVNVPYASLLGVMTANTNDRTTLASYRMVFAF
                     GGSLLVVAIYQPLVDFFKSVVTVETAYQLTMVVIGAIAVLFFFLTFSWTKERIKPPKS
                     QQNSLKEDFKNLLANVPWFVLLGAGVMTLIFNSVRDGVAMYYFKYYIVDDTAVAISKM
                     TFTFSTLYFLVGQAFNMVGVIMAKPVSALLGKRKTFMLAMIGAAVFSSMFYFCNKDNV
                     VLVYVLQSVTSFCAGVIFPLLWSMYADTADYSEWKTGRRATGLVFSASSMSQKLGWTL
                     GGSITLWLLGFYGFQANVEQTPETIEGIKYMLSFFPALGAFLSALFMYFYKLDDNTLK
                     IIETELKQRRDAEALLAE"
BASE COUNT          647 a          415 c          314 g          476 t
ORIGIN      
        1 atatgattaa tgttcttaac aaagcacaaa cgcaaaataa aatttaaaat tcaatgtaac
       61 acattgataa tgttcatatc atcagaaagg ttattttaac taaaatttca tatttccttt
      121 acacaattgt catcaaaaaa aagtaatata aaaggctcca acataaactc atctcaagaa
      181 gacattaaaa aaagagagaa aatacagatc atgcattctc tctcctttca acaattaacg
      241 agcaaaaaca gcacctaaga actattcagc tagcagcgct tcagcgtcac ggcgctgttt
      301 cagttcggtt tcaataatct ttaaagtgtt gtcgtctaac ttatagaaat acatgaacaa
      361 tgctgaaaga aatgccccca atgcgggaaa gaaactaagc atgtatttta tgccttcaat
      421 ggtttcgggg gtttgttcaa cattcgcctg aaaaccataa aaaccaagca gccaaagggt
      481 tattgaaccg ccaagtgtcc accccaattt ttgcgacata ctcgacgccg aaaagaccaa
      541 tcctgtagcc ctacgcccgg ttttccactc tgaataatcg gctgtatcgg catacatcga
      601 ccagagcaag gggaaaataa caccagcaca aaagcttgta acagattgaa gcacatagac
      661 cagtaccaca ttatctttgt tgcaaaaata gaacatactg ctgaacaccg cagcgccaat
      721 catagccagc ataaaagttt tccgtttccc aagcaatgct gaaacaggtt ttgccatgat
      781 gaccccaacc atattaaaag cttgtccaac cagaaaataa agggtactaa acgtaaaagt
      841 catttttgaa atagccacag cggtgtcgtc aactatgtaa tatttaaaat aatacatagc
      901 aaccccatcg cgtaccgaat tgaaaataag cgtcattacc cctgctccaa gcaagacaaa
      961 ccaaggcacg tttgccaata agtttttgaa atcctctttt aatgaattct gttgcgactt
     1021 gggcggttta atacgttctt ttgtccacga gaaggtcaaa aagaagaaga gcaccgcaat
     1081 cgcccctatt acaaccatag tgagctgata agctgtttca acggttacta ccgatttaaa
     1141 aaaatcgaca agaggctgat atatagcaac aacgagtaaa ctccctccaa aagcaaatac
     1201 catgcggtat gatgccaatg tggtacgatc gttagtattg gcagtcatta ctcccagcag
     1261 gctggcataa ggtacattca ccgcggtata agccatcatc ataaacgtat aggtaatata
     1321 ggcatataca aggcgcccgg tagtatcaaa attaggcgta gtaaaaagca aaatacctag
     1381 aataccaaac ggaatggcac cccatagcaa atatggcctg aactttcccc agcgggtgtt
     1441 ggtacggtca ccaataattc ccatgatcgg gtcgttggct gtatcccaaa tccgggttac
     1501 aaggaacatt gtacctgctg caacggccga gataccaaaa atatcggtat aaaaaatagc
     1561 aagataaaat gaaaaaatct tccaaaacat agatgaagca gcatcgccca atccataccc
     1621 tatcttttct ttcaatgaaa cttcacttgt ggccatcatg cttttcatta ttcaatttca
     1681 gttttttcca acctatttca atcgattaac tacttcaatc aaagcccggg tattgtgata
     1741 tgggcatttc cagaaacctg tcttttcatc aatttcgtat ggattgttat ccttatctac
     1801 acgccataac cactctccaa aaacacgatc gattaaataa cggtcaataa aa
//
LOCUS       DAOPUW010000423         1851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_69004_length_1851_cov_2.434298, whole genome shotgun sequence.
ACCESSION   DAOPUW010000423 DAOPUW010000000
VERSION     DAOPUW010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69004_length_1851_cov_2.434298"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1851)
                     /locus_tag="PKJ43_07270"
     CDS             complement(<1..>1851)
                     /locus_tag="PKJ43_07270"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNZ72403.1"
                     /translation="DKGETKTIELINQIHWCQNQFQITQQVTIEGRYKNRYDVTVLVN
                     GLPLVQIELKRRGLELKEAFNQTNRYERDSYGAGQGLFQYIQIFVISNGVNTKYYANS
                     PIKARSFKQTFYWSDERNQLITQLSAFADVFLEPCHISKMITKYVVLNESQKILMVLR
                     PYQYYAVEAIIERVKTTRKFGYIWHTTGSGKTLTSFKTAQILTSFPKVSKVVFVVDRK
                     DLDYQTTKEFNSFCNGSIDGTNNTDTLVKQLSGDNKLIVTTIQKLNTAISKQKYLAKL
                     EPLKDKRIVFIFDECHRSQFGKTHEDIKKFFKGSQMFGFTGTPIFEENAGSNEFGKRT
                     TSMLFGECLHKYVITDAIRDENVLRFSVEYISTFKKKDNILDIDVEAIDEAEVMEAPQ
                     RLNNIADYIIENHQRKTHNREFTAILCVSSVQTLISYYNIFHQKKLNGEHNLKLATIF
                     TWQANEEDQDAVGFVDDDEFLSGDLYSQKLAAEPHAEYSSPGTGDKTQHSRDALEEFI
                     GYYNKEFSTNYTTKDSNSFYNYYNDIAKRVKHKQIDVLLVVNMFLTGFDSKTLNTLYV
                     DKNLKYHGLIQAYSRTNRILNELKSQGNIVCFRNLKSATDQAITLFSNKEA"
BASE COUNT          470 a          358 c          388 g          635 t
ORIGIN      
        1 gcctctttat ttgagaatag agttattgcc tggtcggttg ccgatttcag atttcggaaa
       61 caaacaatgt taccctgcga ttttagctca ttcagtattc ggtttgttcg cgaataagcc
      121 tgaattaagc catgatattt gaggttcttg tcaacgtaaa gggtatttaa ggttttgctg
      181 tcaaagcccg ttaaaaacat gtttacgacc agcaaaacat caatttgttt gtgctttacc
      241 cgctttgcaa tgtcgttgta atagttataa aatgaattgc tgtcctttgt ggtatagttg
      301 gtactaaact ctttgttgta atagccaata aactcttcca gtgcgtcgcg tgaatgctgc
      361 gttttatcgc ccgttccggg cgatgaatat tcagcatgtg gttccgccgc taatttttgg
      421 ctatataaat caccagataa gaattcatca tcatcaacaa atccaacagc atcttgatct
      481 tcttcgttgg cttgccaggt aaaaatggtg gccagtttca aattgtgttc gccgttgagt
      541 ttcttttgat ggaagatatt gtaatagctg atcaatgttt gaacactcga tacgcataag
      601 atggcagtaa attcccgatt gtgtgtttta cgctggtggt tttcgatgat ataatcggca
      661 atgttgttca atcgttgggg ggcttccatt acttctgctt cgtcgatggc ctcaacatct
      721 atatccagaa tattgtcttt ctttttgaag gtgctaatgt attcaactga gaagcgcagc
      781 acattttcat cacgaatcgc atcggtaatc acgtatttat gcaaacattc accaaataac
      841 atggaggtag ttcttttgcc gaattcgtta ctgcctgcgt tttcttcaaa aattggagtg
      901 ccagtaaaac caaacatttg cgaacccttg aagaattttt taatgtcttc gtgggtttta
      961 ccgaattggc tgcggtggca ttcgtcgaaa atgaacacga tccgtttatc ttttaacggt
     1021 tccaattttg caaggtactt ctgtttactg atcgccgtgt ttagcttctg aatagttgta
     1081 acaattagtt tgttgtcgcc cgatagttgt tttaccagtg tgtctgtatt gttggttcca
     1141 tcaatgctac cgttgcaaaa ggagttaaat tctttggtgg tttgatagtc caggtcttta
     1201 cggtcgacaa caaaaacaac tttggaaact ttgggaaaac tggtaaggat ttgggctgtt
     1261 ttgaatgaag ttaatgtttt tcccgaccct gtggtatgcc agatatagcc aaactttcta
     1321 gtggtcttta cccgttctat aatggcctca acggcgtaat actggtatgg ccgtaacacc
     1381 atcagtatct tttgcgattc attcaatacc acatattttg taatcatctt ggagatgtgg
     1441 caaggctcca ggaacacatc ggcaaaagct gatagctgtg tgattagttg gttgcgttca
     1501 tcactccaat agaatgtctg tttgaatgaa cgtgctttaa ttgggctgtt ggcgtagtat
     1561 tttgtgttaa caccattact tatcacaaaa atttgaatgt attggaacaa accctggcca
     1621 gcaccatacg aatcacgttc ataacggttg gtctgattga aggcttcttt cagctccagt
     1681 ccccgccgtt ttaattcaat ttgaaccaat ggtaaaccat ttaccaaaac ggttacatca
     1741 taacggtttt tgtaccgtcc ttcgatggtt acttgttgag taatctggaa ctggttctgg
     1801 caccagtgaa tctgattgat cagctcaatg gtttttgttt cacctttatc g
//
LOCUS       DAOPUW010000424         1844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_69402_length_1844_cov_2.287311, whole genome shotgun sequence.
ACCESSION   DAOPUW010000424 DAOPUW010000000
VERSION     DAOPUW010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69402_length_1844_cov_2.287311"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PKJ43_07275"
     CDS             complement(<1..329)
                     /locus_tag="PKJ43_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72404.1"
                     /translation="MFYELITTDDPLWYKQTVDIYEAVAAGYPANVEELRRGLNDQDA
                     WEQEYELKFLDEATAWLPLELILAAEQEEAGRPELYQGGPCFVGVDIGRRNDLWVCWV
                     DELVCDVA"
     gene            354..578
                     /locus_tag="PKJ43_07280"
     CDS             354..578
                     /locus_tag="PKJ43_07280"
                     /inference="COORDINATES: protein motif:HMM:NF020135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNZ72405.1"
                     /translation="MPDGIVEKTMKAVDSFPADSTSSLTRDVLEGRPSEIEYQNGTIV
                     KLGEKYGVATPVNRYIFESILPREKSARSK"
     gene            complement(604..1476)
                     /locus_tag="PKJ43_07285"
     CDS             complement(604..1476)
                     /locus_tag="PKJ43_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNZ72406.1"
                     /translation="MGGKKVSLVLSSGGARGIAHIAIIDELIAREYEIVSVAGTSMGA
                     FVGGVYASGNLELLKEWMYTLKPVDIVKLFDFSFSSNGLVKGERIFQELGKIFSDVNI
                     EEMPIPYVAISTDIVHNKEIIHDHGSLFNAIRASISIPTFFKPFKKDEHLLVDGGLLN
                     PAPVNRVKRFEGDSLFVVNVETIQSTEPLPIISAELEKMKITESASPLKYHGTPGYIQ
                     IVNKTIGIMLGQLAGLNIEKYHTDLVININRELFGTYDFHKYREIMEVGRLHARAYLD
                     EFEKKMQKSNVPAL"
     assembly_gap    1674..1683
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1729..>1844
                     /locus_tag="PKJ43_07290"
     CDS             1729..>1844
                     /locus_tag="PKJ43_07290"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HNZ72407.1"
                     /translation="MKRAVVLLSGGLDSSVAIHLAKSEGFEVYALSFDYGQK"
BASE COUNT          501 a          421 c          396 g          516 t
ORIGIN      
        1 gcgacgtcgc acaccaactc gtcgacccag caaacccaga ggtcgttgcg gcggccgatg
       61 tcgactccca cgaagcacgg gccaccctgg tacagctctg gacgacccgc ctcttcctgc
      121 tcggcagcca ggatgagttc gagcggcagc caagcggtgg cctcgtccag gaacttcagc
      181 tcgtactcct gctcccaggc gtcctggtcg ttgagccctc gccgcaactc ctcgacgttg
      241 gcggggtagc ctgccgccac ggcctcgtag atgtccacgg tctgcttgta ccagagcgga
      301 tcgtcagtgg tgatcagctc atagaacatg ttgctcttgc cgttggggta aatatgccag
      361 acggtattgt tgagaaaacg atgaaggcgg tcgattcgtt tccggccgat tcaacctcgt
      421 cgcttacccg cgatgtgttg gaaggacgtc cttcagaaat tgaatatcag aatggtacaa
      481 tagttaagct gggtgagaaa tatggtgttg caacccctgt aaaccggtac atttttgaaa
      541 gcattttgcc ccgtgaaaaa tctgcacgat cgaaatagat tttgtcaaac cgaatatctt
      601 ctgctatagt gcagggacgt tacttttttg catctttttt tcaaattcat cgaggtaggc
      661 acgtgcgtgt aaacggccaa cttccataat ttctctgtac ttatgaaaat cgtaagtgcc
      721 aaaaagttct ctatttatgt tgataaccaa atctgtatgg tatttttcaa tattgagtcc
      781 tgcaagttgc cccagcatta tgccaatagt cttgttaaca atttgaatat acccaggcgt
      841 cccgtgatat ttcagcggtg aagcagattc cgtgattttc atcttttcaa gttctgcaga
      901 gattatcggc aagggttcag ttgattggat ggtttctaca ttcactacaa aaagagaatc
      961 gccttcgaag cgtttaaccc tgttgactgg agcggggttc aatagtccgc catcgacaag
     1021 taaatgttca tcttttttaa atggtttgaa aaaagtgggg atcgaaatgg atgcccttat
     1081 cgcattgaac aagcttccgt gatcgtgaat aatctcttta ttatgaacaa tgtcggttga
     1141 aattgccaca tatggaatgg gcatttcttc aatattgaca tcagaaaaaa ttttccctaa
     1201 ttcctgaaaa atacgttcgc ccttaaccaa tccgttagag ctgaatgaaa aatcgaacaa
     1261 tttaacaatg tctaccggtt taagcgtgta catccattct ttcagcagtt cgagattgcc
     1321 cgaggcataa acgccaccaa caaatgcccc catcgaggtt cctgccaccg aaacaatctc
     1381 atattcgcgt gcaataagct cgtcgataat tgcaatgtgg gctatgcccc ttgcgccccc
     1441 gctggataac actagcgata ctttttttcc ccccattggt aattcttgtt ttgatatttt
     1501 ttctgaaaat gaaaggtagt cattaaaatg taattgtcag aaaaaattaa ttatcaaatc
     1561 attatatttg cgaaaaattt cagttaatac tcgacagttc gaaaccctcc tgtcgtaaaa
     1621 taaaactagg tgaaaatgaa acagataaca ctacaaattt tgaatgtggc gacnnnnnnn
     1681 nnngttccca ctattcaatc aacaatcgta caataacttt tcgcaacaat gaaacgcgcg
     1741 gtagttctgc tatcgggcgg attagattcg tcggtagcca ttcatctggc aaaaagtgaa
     1801 gggtttgaag tatatgccct ttcgttcgat tacggacaaa aaca
//
LOCUS       DAOPUW010000425         1841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_69582_length_1841_cov_2.612542, whole genome shotgun sequence.
ACCESSION   DAOPUW010000425 DAOPUW010000000
VERSION     DAOPUW010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69582_length_1841_cov_2.612542"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PKJ43_07295"
     CDS             <1..741
                     /locus_tag="PKJ43_07295"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HNZ72408.1"
                     /translation="FIEETRTIPAFEQIYVDGSFNVFFTHADSSSVRIKCESNILPYI
                     ETAVFNKKLDIKFASHISIMLNEDIDIYITGPYAEKIDLAGSGNITCDSLAAEKGEII
                     ISGSGNIFTNFYGKEFESEISGSGTMDIFGDCDIINTTISGSGSIALEAPDCDFASVT
                     ISGSGSAKLIGSADEVKFINEGSGKIKAYDFPVKKAEIDIEGSGYIYVNVSESLVARI
                     DGSGNIYYIGNPAINFKEFGSGSLINDN"
     gene            879..1328
                     /locus_tag="PKJ43_07300"
     CDS             879..1328
                     /locus_tag="PKJ43_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72409.1"
                     /translation="MAAQKFERSVGLRYGGSNGIFFDVLNEDLSTYRFMLTWREEGRQ
                     VTAMKYYQHYKSDKIPKYLSFYYGFGAHAGYVEWVQYKQDIEHGYYWEDVTSPALGLD
                     GLIGLSYDFERAPVSLLCEVKPYFDIWGKKIFNPVIFDFGICAVYHF"
     gene            1356..>1841
                     /locus_tag="PKJ43_07305"
     CDS             1356..>1841
                     /locus_tag="PKJ43_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72410.1"
                     /translation="MKTLLTSLIILTFTTSLLAQESMTISGDSIFEKNKIKTVFSKEK
                     NDGFYGAFSIGYSPIDDKNALTASARGCWIMDHFFSFGIGGTAFMNQIEEFTSDDILL
                     NNDEVNLAGGYAGIILEPILLPLKPVHLSFPVLIGVGAAGNFSDYTYFSTYMVDDFFW
                     VV"
BASE COUNT          567 a          378 c          377 g          519 t
ORIGIN      
        1 tttattgaag aaacacggac aattccagca ttcgaacaaa tctatgttga cggtagtttc
       61 aatgtctttt tcacccatgc agactcgagc agtgtacgga taaaatgtga atctaacatt
      121 ctgccataca tcgaaacagc cgttttcaac aagaaactcg acataaaatt cgcttcgcac
      181 atcagcatta tgctcaacga agatattgac atatacatta caggacctta tgctgaaaaa
      241 attgaccttg ctggatcagg taatataaca tgcgactcgt tggccgccga aaaaggcgag
      301 ataataatca gcggatcggg aaatattttc accaactttt atggtaaaga gttcgaatcg
      361 gagatttcgg gttcgggtac aatggatatt ttcggagatt gcgacattat aaacacaact
      421 atttcaggat cgggatcaat tgcactggaa gcacctgact gtgattttgc ttcggtaaca
      481 atttccggtt ctggctcggc caaactcatc ggtagtgccg acgaagttaa attcattaat
      541 gaaggctcgg gaaaaatcaa agcctacgac ttccctgtaa aaaaagccga aattgacatt
      601 gagggttcgg gttatatcta tgtaaatgtt tcagaatcac tcgtggccag gattgatgga
      661 agtggcaaca tttattatat tggcaatcct gcaatcaatt ttaaagagtt cggctcaggc
      721 tcgctcatca acgacaacta actaacatca acttgctgca ccaacacaag cgcggcaaag
      781 tataaacgaa agcagttaca acaaatagaa atattaattg aacgcaactg atgaaaaatc
      841 tactcactct catatttatc cttttcttga cacaaccgat ggctgcccaa aaatttgaac
      901 gatcggtcgg gttaaggtat ggcggctcaa acggtatctt tttcgacgtg ctaaacgaag
      961 acctttctac ttaccggttt atgcttacct ggcgcgaaga aggccggcaa gttaccgcca
     1021 tgaaatatta tcaacattac aaaagtgaca aaataccaaa gtatctatcc ttctattatg
     1081 gtttcggagc ccatgccggc tatgtcgaat gggttcagta caaacaagat atcgaacacg
     1141 ggtattattg ggaagatgtt acatcaccag cattagggct tgacgggctt attggtttgt
     1201 cgtatgattt cgaaagggcc ccggtttcac tgctttgcga agtgaagccg tatttcgaca
     1261 tctgggggaa gaaaatcttc aacccagtaa tattcgattt tggcatttgt gcagtatacc
     1321 acttctaacc atgaactatt taaagaaata taatcatgaa aacattgtta actagcctaa
     1381 taatcttgac ttttaccaca agtttgcttg ctcaggaaag catgacgatc agtggagaca
     1441 gcattttcga gaaaaacaaa attaaaaccg ttttttcaaa agaaaagaac gacggtttct
     1501 atggtgcatt ttcaattgga tattcgccca tcgatgataa aaacgccctc accgcatcgg
     1561 cccggggatg ctggatcatg gaccacttct tttcatttgg aattggggga actgcattca
     1621 tgaatcaaat cgaagagttc acatctgatg atattttgct gaacaacgac gaagtaaacc
     1681 ttgcaggagg atatgcaggc attatactcg agcctatcct gctcccgtta aaaccagtac
     1741 atctttcatt tccagtactt attggcgttg gtgcggcagg taatttttct gactacactt
     1801 acttctctac atacatggtt gacgatttct tttgggtagt t
//
LOCUS       DAOPUW010000426         1839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_69683_length_1839_cov_3.395179, whole genome shotgun sequence.
ACCESSION   DAOPUW010000426 DAOPUW010000000
VERSION     DAOPUW010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69683_length_1839_cov_3.395179"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PKJ43_07310"
     CDS             <1..761
                     /locus_tag="PKJ43_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNZ72411.1"
                     /translation="AAIRMVKERGALVLGICNVVGSSIARETDAGIYTHAGVEIGVAS
                     TKAFTAQVTVITLLAMRLARKHKTISDERYFSIINDLLAVPDKIETILMSHLKIRDIA
                     SKYTTSINSLYLGRGYLFPVALEGALKLKEISYIHAEGYAAGEMKHGPIALVDTNLPV
                     IVVAAKDRYYEKIVSNIQEVKARKGNIIAVITEGDNELRNMVNDIFEIPKSDPVLSPL
                     LAIVPLQLFAYYLAVLKGCDVDQPRNLAKSVTVE"
     gene            complement(851..>1839)
                     /locus_tag="PKJ43_07315"
     CDS             complement(851..>1839)
                     /locus_tag="PKJ43_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate permease"
                     /protein_id="HNZ72412.1"
                     /translation="IGTSLKLPLSTTYVTFMVAMSTSLADRAWGRESAVYRISGVVAV
                     IAGWFLTAVITFTVAFFVARIINWGGIIAIAILIALALFLILRSTIIFKRREKIKAEA
                     IEKEDALEQNFSAQKILEKCNKNALKSIISTSKAYYLCFEGFFNDDRLQLKSAVAEAE
                     EIDKKAKKWKDNVFKTIEKLQQDQIDTGHFYVQVVDYLREMAHSLNYTIKPVYTHFEN
                     KHKAFDEEQIEEFNQFRGSLNDFFNYSIYILREKRFDEIDELIEKRVALVNKLKELEK
                     SHIKRIKNKAVSTRNSILYFNIISETKTLLLNLINVVKAQRDFVEETKPYVD"
BASE COUNT          508 a          355 c          394 g          582 t
ORIGIN      
        1 tggctgcaat acgtatggtt aaagaaagag gggccttggt gctgggaatt tgcaatgtgg
       61 tgggttccag cattgcacgc gaaaccgatg caggtattta tactcatgcc ggagtagaaa
      121 taggggttgc ttcaactaaa gcatttactg ctcaggttac tgtaattacc ttgttggcaa
      181 tgcggctggc acgtaaacat aaaacaatat ccgacgaacg atacttctcc attatcaacg
      241 acttacttgc agtacccgat aaaattgaaa cgatattgat gtcgcatttg aagattcgtg
      301 acatagcctc gaaatatacc accagcatca attctttata tttaggaaga ggttaccttt
      361 tccctgttgc gctcgaaggt gctttgaaat taaaagaaat ttcctatatc cacgccgaag
      421 gatatgccgc tggcgagatg aaacacggtc caattgcctt ggtcgataca aatctgcctg
      481 taattgtggt tgccgcaaaa gacaggtatt acgagaagat cgttagcaat atccaagaag
      541 ttaaggcgcg taaagggaac attattgctg taataaccga aggcgacaat gagctcagga
      601 atatggtaaa cgatattttc gaaattccga agtcagatcc cgtactttca cccttgctcg
      661 ccattgtccc cttgcagctt tttgcctatt atcttgcggt gctaaaaggg tgcgatgttg
      721 atcagcctcg aaatctggcc aaatcagtta ctgtagagta acttttttca gcaacataag
      781 ctctctatat tacattatgg aatacaaaaa aacctgccgt cgagcaggtt tttgtatgtg
      841 tgttttgttc ttaatcaaca tatggttttg tttcttcaac aaaatcgcgt tgagctttca
      901 caacgtttat taagttgagc agcaaggttt ttgtttcgga aatgatgttg aagtataaaa
      961 ttgaattacg ggtagataca gccttattct taatcctttt tatatggctc ttttcaagtt
     1021 ccttaagttt gttgaccaat gcaacacgct tttcaataag ttcatctatc tcgtcgaacc
     1081 gtttttcgcg tagaatatat atcgaataat tgaaaaagtc attaaggctg cctctgaact
     1141 ggttgaattc ttcaatttgt tcttcatcga aagctttgtg tttgttttca aaatgggtat
     1201 acaccggttt aatcgtgtaa ttcagcgagt gagccatttc gcgaagatag tcgactacct
     1261 gaacatagaa atggccggta tctatctgat cttgttgtaa cttttcgata gttttgaaaa
     1321 cgttgtcttt ccatttctta gctttcttgt caatttcttc tgcttcggca actgcgcttt
     1381 ttaattgtag cctgtcgtcg ttgaaaaagc cctcgaaaca gaggtaatag gcttttgatg
     1441 tggatattat tgacttgagt gcattcttat tacatttttc gagtattttt tgtgccgaga
     1501 aattttgttc cagagcatct tctttctcaa tcgcttctgc tttgattttt tcacggcgtt
     1561 tgaagatgat tgtcgaccgt aaaattaaga acaaggcaag ggcgataagt atggctatgg
     1621 ctatgattcc gccccagtta atgatgcggg cgacaaagaa tgcaacggta aaggtgataa
     1681 ctgcagtaag aaaccagcca gcaattacgg caactacgcc cgatattcgg tatactgctg
     1741 attcgcggcc ccaggccctg tcggccagtg aggtactcat ggcaaccata aatgttacat
     1801 aagtggttga caagggaagc tttaatgaag ttccaattg
//
LOCUS       DAOPUW010000427         1838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_69750_length_1838_cov_3.486259, whole genome shotgun sequence.
ACCESSION   DAOPUW010000427 DAOPUW010000000
VERSION     DAOPUW010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69750_length_1838_cov_3.486259"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PKJ43_07320"
     CDS             complement(<1..750)
                     /locus_tag="PKJ43_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72413.1"
                     /translation="MQQNSGVDNKGNGADSFLYYNSADFFQLVELLNDSNSVEKCLRN
                     FFDRFTSLFAQFKLTVTVEYDDYCFDSGKSKKSDLYVLHEFTTPEGKSGSLKIGYDRS
                     NVSDNSVFITEITAFLEKIIPVLSARLAVFGYEKLLTFNKERQKELQGINNTTEIIRK
                     SGSLDELFQQICSMLPEAMQYPAHTAVRLVYEGRAYVSDNFKETQWVLKQMFDTPDNK
                     YGSLEVYYLKKFPNAGEGPFLSEERNLINNLA"
     gene            1186..>1838
                     /locus_tag="PKJ43_07325"
     CDS             1186..>1838
                     /locus_tag="PKJ43_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ72414.1"
                     /translation="MGRKKSLLPILLTTMFASFMNPFMLSGVNISLPDIQQFFGCSAT
                     LLSWTINAFLLTNAIILLPVSKLSDQNGRAKYFKIGLYLFTLSSLAAALSPNIPTFIV
                     FRAIQGAGSAMMQVTGMAIVTSAYPPERRGVALGLNIGSVYTGLSVGPFVGGLLTQLG
                     GWQFVFLASVPLAIVAIILAHINLRDNKDELIQTTFDWKGSLIYAAAIVGIVYGGGK"
BASE COUNT          577 a          361 c          334 g          566 t
ORIGIN      
        1 tgcaagattg ttaatcaggt ttcgctcttc acttaggaat ggaccttcgc cagcattggg
       61 gaattttttc agataataaa cttcaagtga accatactta ttatcggggg tatcgaacat
      121 ttgcttgagc acccattggg tttctttgaa attatctgaa acataagccc tcccttcata
      181 aaccaatcgc actgcggtat gtgcaggata ttgcattgcc tcgggcaaca tggagcatat
      241 ctgctgaaaa agctcgtcga gtgaaccact tttccttatt atctcagttg tattattgat
      301 gccttgtaac tctttctggc gttctttatt gaaagtcaac aatttttcat agccaaatac
      361 cgcgagccgg gcacttaata cgggaataat tttttcaaga aatgcagtta tttctgtaat
      421 aaatactgaa ttatcagaaa cattgcttct atcgtaccca atctttaaac taccagattt
      481 tccttcaggt gtggtgaatt catggagaac atacaaatca gactttttac tttttccaga
      541 atcgaaacaa taatcatcgt attcaacagt aactgttagt ttaaactggg caaaaagcga
      601 tgtaaaacga tcaaaaaaat tacgaagaca tttttccaca ctattgctgt cattcaacaa
      661 ttcaaccagt tggaaaaaat cagccgaatt atagtacaaa aagctatctg caccattccc
      721 tttattatcg acacctgaat tctgttgcat cttcgtatga aattaagtat tctggcagtt
      781 tgtctgccca gttcaaattc agttcgaaaa tagtacaaaa aagggagaac attcaaccca
      841 atgaagcaaa atcagcaatc aaaaatccac aaaatatgga ataaaatcat tatacacatc
      901 ggcaaaacta ttggcatttg cttggttcat cctcctgacc attgtgataa aatcgggata
      961 ttaggctttg aaattttcga cataagcaca aataaatttc gagaaattac catacaaaac
     1021 accgtaaatt ctgaggaaaa ttcttcagtc gaacacaata atgcatcgaa tgagttcaac
     1081 atacattcac cttcaaacag aaagcgtaat aaataatgat tcaattgtat atatggtgtt
     1141 tcatttgaat atttttgcac tactaaaaaa tcaaaagaga gaaagatggg aagaaaaaaa
     1201 agtttactgc cgatattact caccacgatg tttgcttcgt ttatgaaccc gtttatgtta
     1261 tcgggagtaa atataagttt acccgatatt cagcagtttt tcggatgtag tgctactctc
     1321 ctaagctgga cgatcaatgc ctttttactc acgaatgcaa ttattttact tccggtgagc
     1381 aaattatcgg atcaaaatgg acgcgctaaa tatttcaaaa ttggacttta ccttttcaca
     1441 ctatcttctc ttgcagcagc attaagtccg aatattccaa cgtttatagt ctttcgtgct
     1501 attcaaggag caggttcagc aatgatgcaa gtaacgggca tggctattgt aacatctgct
     1561 tacccgcccg aaagacgagg tgttgcactt gggttaaaca ttggatcggt gtatactggg
     1621 ttatcggttg gcccgtttgt tggcggtttg ctcacccagc ttggcggatg gcagtttgta
     1681 tttcttgctt ctgtccctct ggcaattgtt gccattatac tggcacacat aaacttgcgc
     1741 gacaataaag atgaattgat ccaaacaact ttcgactgga aaggaagcct tatttatgct
     1801 gcagccatag tcggaattgt ttacggtggg ggcaaaat
//
LOCUS       DAOPUW010000428         1838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_69755_length_1838_cov_3.218732, whole genome shotgun sequence.
ACCESSION   DAOPUW010000428 DAOPUW010000000
VERSION     DAOPUW010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69755_length_1838_cov_3.218732"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PKJ43_07330"
     CDS             <1..238
                     /locus_tag="PKJ43_07330"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNZ72415.1"
                     /translation="IRGLEGVVGPGRIGRELGIDRSFYGRLLEPESLLWIEEDGCRCE
                     VVSTPRIGVAYAGPFWANKPWRFVDKDHFTKFKS"
     gene            320..>1838
                     /locus_tag="PKJ43_07335"
     CDS             320..>1838
                     /locus_tag="PKJ43_07335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNZ72416.1"
                     /translation="MKKWNRKLIVGGSALFVAGFLLMGLNNIDNKNFEIAKNLDIYFS
                     LFRELNTFYVDEIDAGKLVKESIDDMLTSLDPYTTYIPESDIEDFRFMTTGEYGGIGA
                     LISKHGDEILISEPYENFPAHKSGLRAGDVLLEVSGKSTAGMSTEDVSNLLKGSAGQE
                     IKVKIKRYGEKKPLELNIVREKIVIDPISYYGMLDDETGYIYLSNFTKDCGEKVKKTF
                     LELKEEKGAKKLILDLRGNPGGLLMEAVNICNIFIPKDQEVVSTKGKVSEWDKSYKTT
                     GAPVDTLIPIAVIVNRGSASASEIVAGTIQDLDRGVIIGSRTFGKGLVQTTRDLSYNT
                     KLKVTTAKYYIPSGRCIQALDYSHRNEDGSVGHIPDSLISEFSTRNGRKVYDGGGVVP
                     DILVEEKSLSNLSVGLITQFKMFDYATKFRTENATIKSVGAFDINDSIYSDFVNYVEK
                     SNFTYTSETQEVLKELRESAKEEKYLDLAEEQIKELEKTLTPDLDRDLNRFREEIS"
BASE COUNT          561 a          322 c          432 g          523 t
ORIGIN      
        1 gatccggggc cttgaggggg ttgttgggcc gggaagaatt ggccgggaat tagggattga
       61 ccgaagtttt tatggtcgct tgcttgaacc tgaatctttg ctgtggattg aagaagatgg
      121 gtgccgttgc gaggtggttt caacgccccg tatcggagtc gcctacgccg ggcctttttg
      181 ggctaataaa ccatggcgtt ttgtcgataa agatcatttc acaaagttta agagttaagg
      241 tggtgttttc gaacatttga attactattt ttgtcggctg tgtttttgtt gattatacat
      301 ttgaaaaaca aagaataaaa tgaagaaatg gaatagaaaa ttaattgtcg gaggttcggc
      361 tctgtttgtt gcaggctttt tgctcatggg actaaacaat atcgacaata agaatttcga
      421 aattgctaaa aaccttgata tttatttttc tctgttcaga gaactcaata ctttttatgt
      481 cgatgagata gatgccggaa aacttgtgaa agaaagtatc gacgatatgc ttacttctct
      541 cgatccgtac accacttaca ttccggaaag tgatattgaa gacttccgtt ttatgacaac
      601 tggtgaatat gggggtattg gggcgctcat tagcaaacat ggcgatgaga tcctgatttc
      661 ggaaccatac gaaaattttc cagcccataa atcggggctc agggccggcg atgtactcct
      721 cgaagtctca ggaaaatcta ctgcaggaat gtcaaccgaa gatgtaagta acttgcttaa
      781 aggttcagca ggtcaggaaa ttaaagttaa gattaaaagg tatggggaaa agaaacccct
      841 cgaattaaat atagtcaggg aaaagattgt tattgatcca attagctatt acggaatgct
      901 cgatgatgaa accgggtata tttatttatc gaactttacg aaggattgtg gagagaaagt
      961 taaaaagaca tttcttgaat taaaagaaga aaagggagcc aaaaagctta ttctcgacct
     1021 gcgtggcaat cctggtggtt tgctcatgga agcagtcaat atctgtaaca tatttattcc
     1081 taaagaccag gaggttgtga gcacaaaagg taaagtttcg gaatgggata aatcatacaa
     1141 aacaaccggt gcccctgtcg acacattgat ccctatcgcc gtaattgtga accgtggctc
     1201 tgcttcggca tctgaaattg tggctggtac tattcaagac ctcgatcgtg gtgtgataat
     1261 tggttcgaga acttttggta aagggcttgt acaaacgacc cgagacctaa gttataatac
     1321 aaagcttaag gtgactaccg caaaatatta cattcccagt ggacgctgca ttcaggcgct
     1381 tgactattct catcgcaacg aggacggtag tgttgggcat atccccgatt cgcttatcag
     1441 tgaattctct acccgcaatg gaagaaaagt atatgatggc gggggtgtcg tacccgatat
     1501 tcttgttgaa gaaaaaagcc ttagcaattt gtcggttggc ctcattactc agtttaaaat
     1561 gtttgattat gccacgaaat tcagaaccga aaatgcaacg attaagtcag tcggggcttt
     1621 tgatatcaac gattcaattt attctgattt tgtgaattat gtagaaaaat cgaatttcac
     1681 atatacctca gaaacacagg aagtacttaa agaacttcgc gaatcggcaa aagaggaaaa
     1741 atatctcgat ttggctgaag agcaaataaa agaactcgaa aaaacattga ctcctgatct
     1801 cgatcgcgat ttgaaccgtt tcagggaaga gatttcgt
//
LOCUS       DAOPUW010000429         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_69813_length_1837_cov_3.131313, whole genome shotgun sequence.
ACCESSION   DAOPUW010000429 DAOPUW010000000
VERSION     DAOPUW010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69813_length_1837_cov_3.131313"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PKJ43_07340"
     CDS             <1..555
                     /locus_tag="PKJ43_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HNZ72417.1"
                     /translation="PVEVSLLSATNPPDQRFFNQPNIPIALLLEGKFQSAFQNRMTEQ
                     MGIPRSELVKSSPDNKIIVIADGGIIKNKARVRNGQVQMQPLGYDQYSKQTFGNRDFL
                     VNCIDYLSDDSGIMQLRSRVVKLRLLDKVKIRDQKIKWQLINTALPLAFFLIFGLVFS
                     VLRKKKYVNFKNKTNMKNNNNQID"
     gene            751..>1837
                     /gene="dnaN"
                     /locus_tag="PKJ43_07345"
     CDS             751..>1837
                     /gene="dnaN"
                     /locus_tag="PKJ43_07345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNZ72418.1"
                     /translation="MKFVVSSTELLNHLTAISRVISSKSTLPILDNFLFRLEENKLTV
                     TASDLETTLITWIELDNTEGIGEIAIPAKLLIDTLKEFPEQPLIFQVNTETFAIDIFS
                     ENGKFSIMGQNGEDYPGLPELHSSVATSVSMGHDIILSGINKTLFATADDELRPVMNG
                     ILIEISPEDVTFVASDAHKLVRYKRSDFKADIESSFILPKKPSSLLKNLLAKEDFDIK
                     LEFDEKNAFFQLSKFKVICRLVEGKYPSYNSVIPTNNPNRMQVDRLEMYNTLRRVSVF
                     SNAASNLVRLKISGNQLIVSAQDIDFSISAVERINCIYEGDEMEIGFKSSFLLEILSN
                     LSSAEVKVELSDPTRAGILLPAEKEIDD"
BASE COUNT          617 a          388 c          336 g          496 t
ORIGIN      
        1 cctgttgagg tcagcctgtt aagtgcgaca aacccacctg accaacgttt tttcaaccaa
       61 cccaatattc caatagctct tcttcttgaa ggaaaattcc agtcggcatt ccaaaaccga
      121 atgaccgaac aaatgggcat tccacggagc gaactggtaa aaagcagccc cgacaataaa
      181 ataattgtaa tcgcagacgg tggaattatt aaaaacaaag cgcgggtaag aaacggacaa
      241 gtacagatgc aacctcttgg ttatgaccaa tattctaaac aaacattcgg aaaccgcgat
      301 tttctggtca attgtatcga ttatctttcc gacgattcgg gaattatgca attacgttcg
      361 agagttgtaa aactcaggtt actcgacaaa gtgaaaattc gcgaccaaaa aataaagtgg
      421 caattgatta acactgcctt gcctcttgct tttttcctca tatttggcct tgttttttca
      481 gtgctacgaa agaaaaagta tgttaatttt aagaataaaa cgaacatgaa gaataataac
      541 aaccagatag attgaccatt acagcaaaag acaaaataat tgaagtagta ttatcactaa
      601 aataagtacg ctaaaaaaga gaataaaaag gaattggcaa ttttaataaa aaattctact
      661 tttattactt aatcaaaaat tcctttaaga aattcatgta tatttgataa ttatttcgaa
      721 ccttaaataa caagttaaaa tactgatata atgaaatttg tagtttccag caccgaatta
      781 ttgaaccatc tgactgctat cagcagggtt atcagtagca aaagcacctt gcccattctc
      841 gacaattttc ttttccgcct tgaagagaac aaactcactg tcacggcttc ggatttagaa
      901 acaacattga tcacatggat cgaactcgac aataccgagg gcattggaga aattgctatt
      961 ccggcaaaat tgctaatcga tacgttaaag gaatttcctg aacaacccct tatttttcag
     1021 gtgaataccg aaaccttcgc gatcgatatc ttttcagaga acggaaaatt cagcatcatg
     1081 ggccagaatg gagaagatta tcctggcttg cccgagcttc actcgtcagt agctacatcg
     1141 gtcagcatgg ggcacgacat tatcctcagc ggtatcaaca aaacactttt tgccacagcc
     1201 gacgacgaat tgcgcccggt aatgaacggc attttgattg agatttcgcc cgaagatgta
     1261 actttcgtag catccgacgc ccacaaattg gttcgttaca aacgttctga ttttaaagct
     1321 gacatcgaat catcgttcat tcttcctaaa aagccatcat cgctgctgaa aaacttatta
     1381 gcaaaagaag atttcgacat aaaacttgaa ttcgacgaaa aaaatgcctt cttccaactc
     1441 tcgaagttta aggtaatttg ccggctcgtt gaaggaaaat acccaagtta caactcggta
     1501 ataccaacca ataatcctaa caggatgcaa gttgaccgac ttgaaatgta caatacgctc
     1561 cgtcgtgttt cggtattttc gaatgcagca agcaaccttg tacgattaaa aatatctggc
     1621 aatcaactca ttgtttcagc gcaagacatc gacttttcta tttcggccgt agaacgtatc
     1681 aactgcattt acgaaggcga cgaaatggaa attggattta aatcgtcgtt cttgctcgag
     1741 atattgtcaa atttatcatc agcagaagtc aaagtggaac tttctgaccc gacgcgcgcg
     1801 ggcatattgc tccccgccga aaaagaaatc gacgacg
//
LOCUS       DAOPUW010000430         1834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_69983_length_1834_cov_3.406970, whole genome shotgun sequence.
ACCESSION   DAOPUW010000430 DAOPUW010000000
VERSION     DAOPUW010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69983_length_1834_cov_3.406970"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..880)
                     /locus_tag="PKJ43_07350"
     CDS             complement(23..880)
                     /locus_tag="PKJ43_07350"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNZ72419.1"
                     /translation="MKQVWFKQFCKLLFLLLVIAFFPLGIVAQNVEKIGISYQKTGDG
                     LYDMYVENPSFYPVQFLLNFTELDNYETSDPLPYICTLFPGKVKLMTLRRTILDIPGS
                     FKHKYETRVGAYPVNYDQNTVYQLPVPPGKSTKAIWFDLSKSDDPAKIMWAFSLTAGD
                     TVYACREGVVCMLTESKVNNGYRAGENSITVCHPDNSFGKYEVFADSSMFVKVGDKVN
                     TGTPIGLAGGSNYAVGSHVRFSAYYCNVRIDSITGQRLRNYYRYVDPLFQTSDGKTTQ
                     VKADQLYTK"
     gene            1067..>1834
                     /locus_tag="PKJ43_07355"
     CDS             1067..>1834
                     /locus_tag="PKJ43_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ72420.1"
                     /translation="MLMNNKPTISPWGWVPSLYFAQGLPYVIVITVSTIIYKNLGLSN
                     AEITLYTGWLYLPWVIKPFWSPIIDNIRTKRFWVVSTQVVMGVLLAATGLTLPAPGFV
                     KWSLIFFWLIAFSSATHDIAADGFYMLGLDDHQQSFFVGIRNTFWRIAAITGQGGLVY
                     LSGMLINSDIGAKKAWMMLLVATGIIYFSIGIYHKLFLPKPLSDEPRSLHSGTEALKR
                     YFLTFSNFFNRKGIIIFLLFILTYRLGEAQLTKLAAPF"
BASE COUNT          531 a          403 c          347 g          553 t
ORIGIN      
        1 tttaccttcc tttccaccag aatcatttgg tgtataattg atcggcttta acttgggttg
       61 ttttaccgtc agatgtttga aataatggat cgacatacct gtaataatta cgtaatcgct
      121 ggcctgtaat agaatcgatg cggacattac agtaataagc agaaaaacgc acatgcgaac
      181 ccactgcata gttgctgccc cctgcgagcc cgattggggt tcctgtatta actttgtctc
      241 caactttcac gaacatggaa ctgtcggcaa aaacctcgta tttcccaaat gaattatctg
      301 gatggcaaac tgttatcgaa ttttctccgg cccgatatcc gttattgact ttactttcgg
      361 tgagcatgca cacaacccct tcacggcaag catatactgt gtcgccggca gtaagggaaa
      421 atgcccacat tattttagca ggatcatcgc ttttgctcaa gtcgaaccaa atagcttttg
      481 tgcttttccc cggaggcaca ggcaattgat atacagtgtt ctgatcgtaa ttgacagggt
      541 aagctccaac acgggtttcg tatttatgct tgaaactacc agggatatca agaattgtac
      601 ggcgcaaagt catcagtttc accttccccg ggaaaagggt acaaatataa ggcaatgggt
      661 cagatgtctc gtaattatct aattcagtga aatttaaaag aaactgaacc ggataaaaac
      721 ttggattttc aacgtacata tcgtacaagc catcgccagt tttctgatag cttattccaa
      781 ttttttcaac attttgggct acaatcccca acggaaagaa agcaataacc aaaagcagaa
      841 ataacaactt acaaaattgc ttaaaccaca cctgtttcat tttttcaata ttttaattta
      901 ttgattatca aactacattc atcaaaacaa acattaacta tcagttaaag atactcacct
      961 aacgtaaaaa atcaaaccat accattaaca aagaactgat tttttattca ttggacaaac
     1021 ttgaacaata ctacggttgc cggggacaaa aatttttagt tcttttatgc tcatgaacaa
     1081 caaaccaact ataagtccgt ggggctgggt tcccagcctt tattttgctc aaggactccc
     1141 gtatgttatt gttatcaccg tatcgaccat tatttataag aaccttgggt tatcgaatgc
     1201 cgaaatcacg ctatataccg gctggctata tcttccatgg gtaataaaac ctttctggag
     1261 ccctattatt gacaatattc gtaccaaacg tttttgggtg gtaagcaccc aagtggtaat
     1321 gggggtactg cttgcagcaa ccggactcac gttgccggct ccaggctttg tcaaatggag
     1381 ccttatcttc ttttggctca ttgcctttag ctcggctact cacgatattg ctgccgatgg
     1441 tttctacatg ctgggcctcg acgaccatca gcaatcgttc tttgtaggca tacgcaatac
     1501 cttttggcga atagcggcta ttacagggca aggcgggctg gtatatcttt cgggaatgct
     1561 aatcaattcg gatatcgggg caaaaaaagc ctggatgatg ttgcttgttg ctaccgggat
     1621 tatttatttt tcaattggga tttatcacaa attattctta cccaagccat tgagcgatga
     1681 gccacgaagt ttacatagtg gtacagaagc actaaaaaga tacttcctta ctttttccaa
     1741 tttttttaac cgtaaaggca tcatcatctt tctattattc attttgactt accgactggg
     1801 tgaagcccag ctaacaaaac tggcagcccc tttc
//
LOCUS       DAOPUW010000431         1834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_69986_length_1834_cov_3.358628, whole genome shotgun sequence.
ACCESSION   DAOPUW010000431 DAOPUW010000000
VERSION     DAOPUW010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69986_length_1834_cov_3.358628"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PKJ43_07360"
     CDS             complement(<1..639)
                     /locus_tag="PKJ43_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNZ72421.1"
                     /translation="MNLNNFTIKGRDTINQAAQIAASSGQQAIETGHLLKALQQIAEN
                     ITGYIFNKLSINSNALNLALDRIIDGYSKVSGGQPYLSNAANSAIQKAIKYASDMGDQ
                     FVSIEHLLLGLLEAGDQVSSLMKDSGFAKNDLRSAIEELRKGAKADSETAEDQFNSLG
                     RFAINLVDQARKGKLDPVIGRDDEIRRLLQILTRRTKNNPLLIGEPGTGKTAI"
     gene            1208..>1834
                     /locus_tag="PKJ43_07365"
     CDS             1208..>1834
                     /locus_tag="PKJ43_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNZ72422.1"
                     /translation="MATKYVYTFGKGQAEGKADMKELLGGKGANLAEMNLIGVPVPPG
                     FTITTEVCTIYNEKGKDVAFNMIKGEVEKAVAHIEKLTGTKFGDKKNPCLVSVRSGAR
                     ASMPGMMDTILNLGMNDDAVEGITAKSGNPRFAWDSYRRFVQMYGDVVLGMKPVNKED
                     IDPFEAIIEELKHEKGIVSDQDFSTADLITLVSRFKAAVKKNTGHDFPA"
BASE COUNT          614 a          340 c          381 g          499 t
ORIGIN      
        1 aatggcggtt ttcccggttc ccggttcgcc aataagaagc gggttatttt tggtacggcg
       61 agtgagaatc tgtaacaaac gtcgaatttc atcatcgcgc ccaattaccg ggtcaagttt
      121 tcctttccgt gcctgatcga caaggttaat ggcgaatcgg ccaagagaat tgaactggtc
      181 ttctgctgtt tcgctatctg cttttgcccc ttttctgagt tcctcaatcg cacttcggag
      241 atcatttttg gcaaagccac tatctttcat tagcgaagaa acctgatcgc cagcctcgag
      301 caaacccaat aacaaatgtt cgatcgatac aaactggtcg cccatgtctg atgcatattt
      361 gatggctttc tgaatagcag aattggctgc attcgacaag taaggctgcc ctccgcttac
      421 tttagagtat ccatcgatga ttctgtcgag tgcaagattt agtgcattcg aatttatgct
      481 caatttgtta aatatatatc cggtaatatt ttcagcaatt tgctgcaacg cttttagcaa
      541 gtgcccggtt tcaattgcct gctgaccaga tgaagcagca atctgagctg cctgattaat
      601 ggtgtcgcgt ccttttatgg taaaattatt caagttcata tcttattttt attgatttac
      661 aaattattac gatcaaaaca aacgatttgt ttcactacct aaactccgta tataaaggct
      721 cgaagaatag cgcgaataac aaaacgttga agcaacaaat acttatccaa cagggaaata
      781 ctgaaaaaat gacatgaaga acattgaaaa tagcgacata aaagcaggga ataggagagt
      841 atgcgaaaaa ttggcacaaa aaaaaggatg accatcgcca tcctctaaac ctaactaaac
      901 ctaaatctat atgaaaaatc atctatctta aacacaaaac aaaggtacgc taaataagtg
      961 ttagcaattg ataatcaaag ttaatatttg ttaacagtga tcttttcatc aaacaaggga
     1021 catagaataa tttgagaaga ggagtgacta aaaaacaaaa aggttaactt tgaaaacgtt
     1081 tttagaagca gatttcgaaa aagaattaaa aatggccaaa aacaaagcac attctatacc
     1141 tgattaaaaa gtattgaata aatttggggg cgattaaaaa acacaattaa attaagatca
     1201 taaaattatg gcgacaaagt acgtttatac ttttggcaaa ggtcaggcag aaggcaaagc
     1261 tgacatgaaa gagttattag ggggtaaagg tgcaaacctt gccgaaatga accttatcgg
     1321 ggttccggtt cctccgggct ttacaattac caccgaggta tgcactatct acaatgaaaa
     1381 agggaaagat gttgctttta acatgattaa gggagaagta gagaaggcag ttgcacacat
     1441 cgaaaaatta acaggaacaa aatttggaga taaaaagaac ccatgtttgg tttcggtacg
     1501 ttcgggagca agagcttcga tgcccgggat gatggatact atccttaatt tgggtatgaa
     1561 cgacgatgcc gttgaaggga taaccgcaaa atcgggaaat cctcgctttg cctgggactc
     1621 gtaccgtcgt tttgtacaga tgtatggcga tgttgtttta ggaatgaaac cggtgaacaa
     1681 agaagatatc gatccattcg aagcaattat tgaagagttg aaacacgaaa aagggattgt
     1741 atcagaccag gatttctcta ccgccgactt aatcacatta gtgtcgcgtt tcaaagctgc
     1801 tgttaagaaa aatacaggtc acgattttcc agcc
//
LOCUS       DAOPUW010000432         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_70229_length_1830_cov_3.613521, whole genome shotgun sequence.
ACCESSION   DAOPUW010000432 DAOPUW010000000
VERSION     DAOPUW010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70229_length_1830_cov_3.613521"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="PKJ43_07370"
     CDS             complement(<1..1328)
                     /locus_tag="PKJ43_07370"
                     /inference="COORDINATES: protein motif:HMM:NF024295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HNZ72423.1"
                     /translation="MKIILSFWLVAVSFVAFAQDVVIEVDASKGQRQVSPYIYGRNNN
                     FSNVFGTPTSAAEIALYKEAGLRFARENTGNNATKYNWRLKISSHPDWYNNVYDHDWD
                     FAAQTIEKNMPDLQAMWAFQLIGKVAGSKRYNFNDWSYNSSQYWSGCSQNLAGGGVVN
                     PNGEKALTNGDPNLYLVDWPADSTTEILMHWFGNDGIGLNPDQFRYWSMDNEPEIWNG
                     THDDVMPSQISANEFMNRYFEVAKLARAKFPNIKLAGPVPANEWQWYKYANESLKIGG
                     TYYCWLEYFIKRVADEQKASGIRLLDVLDIHWYPSESKDADVLQLHRIFFDETYAYPG
                     ANGLKSLTGGWTNNASKEYIFKRINTWLDKHFGEGHGISLALSESGINSSNPNVNSVL
                     YASMLGTFASNGVEIFTPWTWKTGMWETLHLFSRYAKNVSVNTTSSLENMV"
     gene            1645..>1830
                     /locus_tag="PKJ43_07375"
     CDS             1645..>1830
                     /locus_tag="PKJ43_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ72424.1"
                     /translation="MIKNSFGSCRYISMQQTENIITIEHISQIFGEKVVLDDINLSVR
                     KGEFVTILGPSGCGKTTL"
BASE COUNT          560 a          400 c          332 g          538 t
ORIGIN      
        1 accatattct cgagcgaaga ggttgtattc acgctaacat tttttgcata acggctaaag
       61 aggtgcagtg tttcccacat tcctgttttc catgtccaag gagtaaaaat ttcgactcca
      121 ttgcttgcaa aagtgcctaa cattgaagca tatagcacgg agtttacatt aggattacta
      181 ctattgatac cactttcaga aagcgcaagg cttatgccat gtccttcgcc aaaatgttta
      241 tcgagccaag tatttatccg tttgaaaata tattctttac tggcattatt agtccaacct
      301 ccggttaggg acttcaaccc gtttgccccg gggtaagcat acgtttcgtc gaaaaaaatt
      361 cggtgcaact gcaacacatc tgcatcttta ctttcactag gataccagtg aatatcaagc
      421 acatcaagca atcggattcc cgaagctttt tgctcatcgg ccacgcgttt tataaaatat
      481 tcaagccagc aataataggt cccgccaatt tttaacgact cgtttgcata cttgtaccat
      541 tgccattcgt tggccggaac ggggcctgca agctttatgt tagggaactt cgcacgagcc
      601 aatttagcaa cttcgaaata acggttcatg aattcgttcg cagagatttg gctgggcata
      661 acatcgtcgt gtgttccatt ccaaatttca ggttcgttat ccatactcca atatctgaat
      721 tgatcgggat taaggccaat gccatcatta ccaaaccagt gcattagtat ctcggtagtt
      781 gaatcggcag gccaatcaac aaggtaaaga ttaggatcac cattggtgag tgctttctca
      841 ccattcgggt tcaccactcc gcctccagcc agattttgac tgcaaccact ccaatactgg
      901 gaactgttgt aggaccaatc gttgaaatta taacgcttgc taccagccac tttaccaata
      961 agctgaaatg cccacattgc ctgaagatcg ggcatgtttt tttctatggt ctgggctgca
     1021 aaatcccaat cgtggtcata tacattgtta taccaatcgg gatgactaga aattttcagc
     1081 ctccaattat atttagtggc attgtttcca gtattttcgc gggcaaaacg caaaccggct
     1141 tctttataca acgcaatttc tgctgccgag gtaggtgtgc caaaaacatt cgaaaaatta
     1201 ttgttacggc cataaatgta cggtgaaacc tgtcgctgtc cttttgaggc atcaacttca
     1261 attacaacat cctgcgcaaa agcgacaaaa gaaacggcaa caagccaaaa cgataaaata
     1321 attttcataa aactatattt tatatgttct tgtttttctg ctaattgcaa aagtactctc
     1381 cctaattact catcgaaagt aagttattga aatcaacgct gaaaattaca aagaacaaat
     1441 ttaatacgca tgatcaatat gttttcaaac acgtaatagt tttgctcata aagcaattat
     1501 tgcttgcctc taaaaaggat caatcggcta aagtaatcat ctgatgtaaa atcaactagt
     1561 aaagaatacg cacggcgttc aattcgaaga ccaaaaaccc ttttcatttt catttcatta
     1621 tctatatttg tctgctaata aaaagtgata aagaattcat tcggttcatg ccgctatatt
     1681 tcgatgcaac aaactgaaaa tattattaca attgagcaca tttcgcaaat atttggcgaa
     1741 aaagtagttc tcgacgatat aaacctctcg gtacgcaaag gtgaatttgt aacaatcctt
     1801 gggccatcgg gatgcggaaa aacaaccctg
//
LOCUS       DAOPUW010000433         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_70234_length_1830_cov_3.379155, whole genome shotgun sequence.
ACCESSION   DAOPUW010000433 DAOPUW010000000
VERSION     DAOPUW010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70234_length_1830_cov_3.379155"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1830)
                     /locus_tag="PKJ43_07380"
     CDS             complement(<1..>1830)
                     /locus_tag="PKJ43_07380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ72425.1"
                     /translation="DHKKQIWVGTIGGGLNLFDPQKEQFKRFSEHDGLPENTIRSITE
                     DKNNDLWISTNLGIARLNYETFEIKSYGGKTIQQMGEFLISSSTTDNLGNVYFGASNG
                     FIRFNPNEITENKNIPPVYFSNFRLFNKTVEIGKKNSPLKKHISLTNEIELEHKQSVF
                     SIDFVALNYTEPGQNQYAYYLEGLEPDWNYSGHTNSATYTNLNPGKYTFKVKASNNDG
                     KWNNTPTTLIIDVNPPAWATWWAFSIYLVLLSLIFYSIIRLIQNRAIEQEQIRAERIQ
                     NQNLQELKDRKLKFFTNISHEIRTPLSLIITPLEELLHTPGLSNDIKKKIRYAGDNSK
                     FLLKLVNQLLDFRKLDNSMMELRVCKTSINQLVEQIIALHKLRAEEKEIELVFEPLET
                     DLNFPVDTDKVEKIIHNLLSNAVKFSPDNSLIFIRVNENKASEQLIFEVIDHGPGIEP
                     DQIPLIFERFYQGTSAFNKGGTGIGLALCKELAEIHKGQLEVESVKGEGCNFKLILPT
                     NPEVYNDAVKITDKQIIEEPDSECTQPTPYEIEEESSQLKSIRYSMVIVEDDSQIRNY
                     LSDEFSKTYKTHTASNGKEALKLINSVMPDIIITDVLMPEMDG"
BASE COUNT          453 a          336 c          383 g          658 t
ORIGIN      
        1 ttccatccat ttcgggcatt aatacgtcgg ttatgatgat atcaggcatc acactgttta
       61 taagcttcaa cgcttctttg ccgttagatg cagtgtgtgt cttgtatgtt tttgaaaact
      121 cgtcgctgag gtaattccgt atttgagaat cgtcttcaac gatgaccatt gaatatctga
      181 tgctttttaa ctgacttgat tcttcttcaa tctcatatgg ggtaggttgt gtacattcag
      241 agtcaggttc ttcaataatc tgtttgtcgg taatttttac cgcatcgttg tatacttcgg
      301 ggttggttgg caaaatcagc ttgaagttac atccttcgcc tttaacgctt tcgacttcga
      361 gctgaccttt gtgaatctca gcaagttctt tgcagagggc caacccaatt cccgtacctc
      421 ctttattaaa ggccgaagta ccttggtaga aacgttcaaa aatcaggggt atctggtctg
      481 gttcaatgcc tggcccatgg tcaataactt cgaaaattaa ctgctcgctt gctttatttt
      541 cgttaactct tatgaaaatc aaggaattgt ctggtgaaaa tttaacagca ttagataaga
      601 ggttgtgaat aattttctcc actttatcgg tatcgaccgg aaaatttaaa tcggtttcta
      661 ggggttcaaa tactaattct atttcttttt cttcggcacg aagtttatgt agggcaatta
      721 tttgttcaac gagttgatta atacttgttt tgcaaaccct gagttccatc atgctgttgt
      781 cgagttttct gaaatcgagc aactgattta ccaattttag caggaatttt gaattgtcgc
      841 cagcgtaacg gatctttttc tttatatcgt tacttaagcc cggagtgtgg agtaattcct
      901 ccaaaggtgt gattattagc gataatggtg ttcttatttc gtgcgatata tttgtaaaaa
      961 actttaattt tcggtctttt agttcctgta ggttctggtt ttgtattcgt tcagccctta
     1021 tttgttcttg ttcaattgcc ctgttctgaa taaggcggat gatggaatag aaaatgaggg
     1081 aaagaagcac taaatatatc gaaaaagccc accatgtagc ccatgccgga gggtttacat
     1141 caataatcaa ggtcgtgggt gtgttgttcc atttgccgtc gttgttcgaa gcttttactt
     1201 taaaagtata ttttccaggg ttcaggttgg tgtaggttgc cgaatttgtg tgccctgagt
     1261 aattccagtc aggctccagg ccctcgaggt agtatgcata ttggttttga cctggttctg
     1321 tgtagtttag tgcaacaaaa tcgatagaaa aaacagactg tttatgttca agttcgatct
     1381 cgttggttag cgaaatatgc tttttcagcg gggagttttt cttcccgatt tccactgttt
     1441 tgttgaaaag cctgaaattc gaaaaataaa cgggcggaat atttttattc tccgttatct
     1501 cgttgggatt gaatcgtata aaaccattgc tggctccaaa atatacgttc cccaagttat
     1561 ctgttgtgga ggagctaata agaaattcgc ccatctgctg aatggttttt cctccgtatg
     1621 attttatttc aaaggtttcg tagttcaatc tggctatacc tagattggtg ctgatccata
     1681 ggtcgttatt tttatcttca gtaatacttc ttattgtgtt ttcgggcaat ccatcatgtt
     1741 ccgagaaacg tttgaactgt tctttttgtg gatcaaaaag gttaagtccg ccaccaatag
     1801 tgccaaccca aatctgtttt ttgtgatctt
//
LOCUS       DAOPUW010000434         1828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_70357_length_1828_cov_3.007896, whole genome shotgun sequence.
ACCESSION   DAOPUW010000434 DAOPUW010000000
VERSION     DAOPUW010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70357_length_1828_cov_3.007896"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PKJ43_07385"
     CDS             <1..328
                     /locus_tag="PKJ43_07385"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNZ72426.1"
                     /translation="EIDQRVGKLENDWVIQKEHGNAFQDTCLKTILLETGIDQIAVGG
                     LVSHGCVKATCLGGLAEGYDVSLLKNGHTNWNKDAKEKIEQTENELATKGVKILDIAS
                     LKEPVK"
     gene            325..1188
                     /locus_tag="PKJ43_07390"
     CDS             325..1188
                     /locus_tag="PKJ43_07390"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNZ72427.1"
                     /translation="MTNKEVILKAVKFIEGNLKEEIDVFKVSREVCYSLYHFIRLFQS
                     ITGFSPKNYIQQRRLTEAVYELRNSDKKIVDIAYDFQFGSHESFTRAFRKQFHINPSE
                     IRNGYSLTSMSLVNPITEDYIFQSDIVRNQPPDLVELPEKILVGISFFISDDAEITSL
                     SKEWNRFINEAGTIKNKTVPECFYQVQYWSDNQELGGLYFFTGTEVTEVTDLNPLFVV
                     KIIPKGKYLRFIHKGFSNKVGYTYNYIYTQFLPETDYKLTKPFNFEYYGELYKGACND
                     QSESEIYIPVE"
     gene            1206..>1828
                     /locus_tag="PKJ43_07395"
     CDS             1206..>1828
                     /locus_tag="PKJ43_07395"
                     /inference="COORDINATES: protein motif:HMM:NF025589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HNZ72428.1"
                     /translation="MGYYTDLATISIDDYQAKLESADLLPSRMLLKERLAERFGYFKS
                     IGIRNVLDLQKLLKKKEKLAELTKVDGFSEDFLVVLLREINSIQPKPNKLNEFFGISS
                     ETVSKLEKNGIKDTLKLFDHVLTPQSRKKLAAETNIPEAEILELAKLTDLSRIKWVGA
                     TFARMLYEVGVDTAEKASKTDYVELHQKIFQLNKEKNFYKGQIGLHD"
BASE COUNT          582 a          367 c          406 g          473 t
ORIGIN      
        1 ggaaatcgat cagagagtag gtaagctgga aaatgattgg gtaattcaga aagaacacgg
       61 aaatgctttt caggatacct gtttgaaaac tattctttta gagacaggta ttgatcaaat
      121 tgccgttgga ggattggtaa gccatggttg tgtaaaagcg acttgtttag gcggattggc
      181 agaagggtat gatgtgtcgc ttctgaagaa tggtcatact aattggaata aggatgcaaa
      241 agaaaagatt gaacaaaccg aaaatgaatt ggcaactaaa ggagtgaaaa tcctggatat
      301 tgcttcgcta aaagaaccag tgaaatgaca aacaaagagg tgatattgaa ggccgtaaag
      361 tttattgaag gcaacctgaa agaggaaatc gatgttttca aggtttcacg cgaggtgtgc
      421 tattcgcttt accatttcat caggctcttc cagagcataa caggtttttc gccaaagaac
      481 tacatccaac agcgcaggct taccgaggcg gtgtacgagt tgcgcaattc cgataaaaaa
      541 atcgtcgata ttgcttacga ttttcagttt ggaagccacg aatcgtttac gcgagcgttc
      601 cggaagcagt tccacattaa cccctccgaa ataaggaacg ggtattccct gacttccatg
      661 tcgctggtca atcctattac cgaagattac attttccaat cggatatagt gcggaatcaa
      721 ccgcctgatc tggttgaact tcctgaaaag atactggttg gcatctcttt ttttatcagc
      781 gacgatgctg aaattaccag cctttcgaaa gagtggaacc ggtttatcaa cgaagctggt
      841 acgataaaga acaaaacagt tccagaatgt ttttatcagg ttcagtattg gtccgacaat
      901 caggaattgg gcggactcta cttttttaca ggaactgaag tgaccgaggt aaccgacctg
      961 aacccgttgt ttgtggttaa aatcatcccc aaagggaagt atttgcgctt tatccacaaa
     1021 ggcttttcga ataaggttgg ctacacgtac aactacattt acacgcaatt cttacccgaa
     1081 accgattaca aactaaccaa accctttaac tttgagtatt atggcgaatt gtacaaaggt
     1141 gcctgtaacg accaatcgga aagcgaaatt tacattcctg ttgaataatt taaaccgtta
     1201 acaccatggg gtattatacc gatttagcca ctattagcat cgacgattat caggcaaagc
     1261 ttgaatcggc cgatttgctg cccagccgta tgttgcttaa agagcggttg gcagaaaggt
     1321 ttggctactt taaaagcatc ggcatcagga atgttcttga tttgcagaaa ctcctcaaaa
     1381 agaaagaaaa actggccgaa ctgacaaagg tggatggttt ctcagaagat ttcctggttg
     1441 ttttgctccg cgaaatcaac agcattcagc cgaaaccgaa taagctaaat gaattctttg
     1501 gcatttcgtc cgaaaccgtt tccaaactgg aaaaaaacgg gataaaagat acacttaagt
     1561 tattcgatca tgtgttaacc ccacaaagca ggaagaagct tgccgcagaa acaaacatcc
     1621 ctgaagccga aattttggaa ctggccaaac taaccgacct ttcgcgcatt aaatgggttg
     1681 gtgctacctt tgcccgtatg ctttacgagg taggggttga caccgccgaa aaagcatcaa
     1741 aaaccgatta cgtggagctt caccaaaaga ttttccaact caataaagaa aagaatttct
     1801 acaaaggcca aatcgggctg cacgacat
//
LOCUS       DAOPUW010000435         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_70443_length_1827_cov_2.102709, whole genome shotgun sequence.
ACCESSION   DAOPUW010000435 DAOPUW010000000
VERSION     DAOPUW010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70443_length_1827_cov_2.102709"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="PKJ43_07400"
     CDS             <1..1158
                     /locus_tag="PKJ43_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HNZ72429.1"
                     /translation="FDYSKALPNGKHAANNEKVKRYIDFAAEHGFSQVLVEGWNIGWE
                     DWFGKSKDYVFDFVTPYPDFDVKMLQGYAKSKGVRLMMHHETSSSVRNYERHMDKAYQ
                     FMVDNGYNSVKSGYVGDIIPRGEYHYGQWMVNHYQYAIEKAAKYKIMVNAHEAVRPTG
                     LCRTYPNLIGNESSRGTEYESFGGSKPFHTTILPFTRLIGGPMDYTPGIFEMDMTKLN
                     PDNNSHVNSTLARQLALYVTMYSPLQMAADLPENYMRFPDAFQFIKDVAIDWDETRAL
                     AAEPGDYITYARKAKGTNNWFIGTTVDENGYVSNLSFDFLDEGKQYIATIYADAPDAH
                     YKTNPQAYQIRKVVVNKKSKLVQKCAPGGGYAISVIEVSDKAQTKGLKVLK"
     gene            1306..>1827
                     /locus_tag="PKJ43_07405"
     CDS             1306..>1827
                     /locus_tag="PKJ43_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72430.1"
                     /translation="MIDSKEQRIIEFLKKEGESSSKEIFDRVEIAVSYATLKRILAKL
                     IAENYLSSSGKGKGTKYSISSIYEVIQPIDTSLYFEKEIDERKIKEAFNIEIICDVLA
                     KNCVFTHNETQKLNELQAKFLANISQLSETEFKKEFERLAIDLSWKSSQIEGNTYSLL
                     ETEHLLKEKEAAAG"
BASE COUNT          612 a          348 c          408 g          459 t
ORIGIN      
        1 ttcgattata gcaaggcctt acccaatggc aagcatgcag caaacaacga gaaagttaaa
       61 cgctacatcg attttgctgc tgaacatggc ttcagtcagg tattggtaga aggttggaat
      121 attggctggg aagactggtt tggaaaatcg aaagactatg tgttcgactt cgttactcct
      181 taccccgatt tcgatgtgaa aatgcttcaa ggctatgcta agtcaaaagg ggtgcgttta
      241 atgatgcacc acgaaacatc aagttcggtg cgcaactacg aacgccatat ggacaaagca
      301 taccagttta tggttgataa cggctacaac tcggtaaaaa gcggatatgt aggcgacatc
      361 ataccgcgcg gagaatatca ctacggacag tggatggtga accattatca atatgccatt
      421 gaaaaagctg ccaaatacaa aataatggtc aatgcccacg aggctgtgcg ccctacaggt
      481 ttgtgccgca cctaccctaa ccttatcgga aatgaatcat caaggggaac agaatacgag
      541 tcgtttggag ggagcaaacc tttccatact accatccttc cgtttacgag gttgattgga
      601 ggaccaatgg attacacccc aggtattttc gaaatggata tgaccaaatt gaacccagac
      661 aataattcgc atgtaaactc aacgttggca aggcagttgg cactttacgt taccatgtac
      721 agcccacttc aaatggccgc agacttgccc gaaaactaca tgcgtttccc tgatgcattc
      781 cagttcatta aagatgttgc tattgattgg gacgagaccc gtgctttggc cgctgagcca
      841 ggagattata ttacttatgc ccgcaaagct aaagggacaa acaattggtt tattggcaca
      901 acagtcgatg agaacgggta tgtctctaat ttgagtttcg attttcttga cgaaggcaaa
      961 caatacattg ccaccattta tgccgatgct cccgatgcgc attacaaaac caatccacaa
     1021 gcctaccaaa taagaaaagt ggtggtaaac aagaaatcga aactggtgca aaagtgtgca
     1081 ccgggcggag gatatgccat cagtgtgatt gaagtaagcg acaaggcaca aacaaaggga
     1141 ttgaaagtgt tgaaatagta tagattgaag agctgaacga ggtgcagaaa acatgccccg
     1201 ttcaactctc aattaaattt gtagctcaca caagttgaaa atgtggctca aaaatcaatg
     1261 gaaatgagct ttattgagta tatttatgag ctaaaagagt gagtgatgat cgattccaaa
     1321 gagcaaagaa ttattgaatt tctgaaaaaa gagggggaaa gctcgtcaaa agaaattttc
     1381 gaccgggttg aaatagccgt tagttatgca actttgaaga ggattttagc taagttgatt
     1441 gcagaaaatt atttgtcttc ttcaggaaaa gggaaaggta caaaatacag tatttcttct
     1501 atttacgaag ttattcagcc aattgataca agtctgtatt ttgaaaaaga aattgacgaa
     1561 cggaaaataa aagaagcgtt caatattgaa attatatgcg acgttttggc taaaaactgt
     1621 gttttcacgc ataatgaaac acagaagctc aatgaacttc aagctaaatt tctggctaac
     1681 atttcccaac tatcagaaac agaattcaaa aaagagtttg agcgcttggc aatcgactta
     1741 agctggaaat cgtcgcaaat tgaaggaaac acctattctt tgttggaaac cgagcatctc
     1801 ttgaaagaaa aagaagccgc agcaggg
//
LOCUS       DAOPUW010000436         1826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_70480_length_1826_cov_3.446076, whole genome shotgun sequence.
ACCESSION   DAOPUW010000436 DAOPUW010000000
VERSION     DAOPUW010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70480_length_1826_cov_3.446076"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PKJ43_07410"
     CDS             complement(<1..861)
                     /locus_tag="PKJ43_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNZ72431.1"
                     /translation="MLTLKLIQSNPDFVIERLKVKNFDATQIINQVNELNTQRKLTQT
                     RVDNLKAEMNNLSKEIGNLFKEGKTSEANAAKEKTAELKEEIKNLDQTFAEIDDKLNN
                     LVVMLPNIPHQLVPEGRTAEDNLEIRRGGSIPALPENKMPHWDLIKKYDIIDFELGIK
                     LTGAGFPVYKGKGAKLQRALINFFLDEGEKAGYREVQPPIMVNEDSGFGTGQLPDKEG
                     QMYHATADNLYLVPTAEVPVTNIYRDEILEEAELPIKNMAYTPCFRREAGSWGAHVRG
                     LNRLHQFDEVE"
     gene            1364..1813
                     /locus_tag="PKJ43_07415"
     CDS             1364..1813
                     /locus_tag="PKJ43_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HNZ72432.1"
                     /translation="MNGIDNSITVIRSEKLSKTGMVSHFFSTRKGGVSSGVFSSLNFG
                     SQHGEASNMVRNIDLLRGEVGCKTSRFVISKQTHSDVVAVVGVNNLDNCFDSTDALIT
                     NLPNVFVCVKTADCVPILLFDPVLRVVAAVHSGWRGTVQILLVNQLR"
BASE COUNT          459 a          337 c          412 g          618 t
ORIGIN      
        1 ttcaacttcg tcgaattggt gcaaccggtt aagcccgcgt acatgggcac cccatgaacc
       61 ggcttcgcgg cggaaacatg gtgtgtaggc catattcttg ataggaagtt cagcttcttc
      121 taaaatttca tcgcggtata tgttggtaac tggcacttcg gctgttggaa cgaggtagag
      181 attatcggcg gtggcatggt acatttgccc ttctttgtcg ggaagctgtc ctgtgccaaa
      241 gcctgaatct tcgtttacca taattggtgg ttgtacttca cgatagccgg ccttttcccc
      301 ttcgtcgagg aaaaagttga tcaatgcacg ctgtaatttt gccccttttc ctttatagac
      361 agggaatcct gctccggtaa gttttatccc aagctcgaag tcaatgatgt cgtatttttt
      421 aataaggtcc cagtgaggca tcttgttttc gggcagggca gggattgaac ctccgcgtct
      481 gatttcaaga ttgtcttcgg ctgtacgtcc ttcaggaact aactgatggg gaatgttcgg
      541 aagcattaca accaggttgt tcagtttgtc gtcgatctct gcaaaagtct gatccaggtt
      601 tttgatttcc tctttcagtt ctgctgtttt ttcttttgca gcattagcct ccgatgtttt
      661 tccttctttg aataggttcc ctatttcttt cgaaagattg ttcatttctg ctttcaggtt
      721 gtcgacccgg gtttgtgtca acttgcgctg agtgttcagc tcgtttacct gatttattat
      781 ttgggtggca tcaaaattct ttactttaag cctctcgatt acaaaatcgg gattgctctg
      841 aatgagttta agcgttagca ttccactgta tttttaacaa ttaacttaag tgcaaaaata
      901 gtaattcgcc tgtcatcggt acgatatttt agtaaaaagt aaataagctt tgttgagaat
      961 taaactcaaa aagtgagttt ctgcagatcg acgttttctc aggaaagaat gatgctgata
     1021 gttttcacgg ttacatcgat tttattcttt taaatagcag caagaggaac tgccgacgag
     1081 gggtcgataa ttttttcatt cgttcctata ccatatgcat tgtgttaact atgcagtctt
     1141 ccgaaacagt aacgacttat cgacttgctt tctatgattt caatcttgat tttgtttata
     1201 cttgcactcg aaatcagagg agcagggtga ataattaggt aaatatgccg gtgtgcttat
     1261 tcaatagtta tataatcacg aagttatgcc aggttattca tggtgtattc tgaaaaatgt
     1321 ttgtctacct aaaaatttta ttcagaaaag gaaaaaactg ataatgaacg gcatcgataa
     1381 ttcaattacg gtaatcagat ctgagaaact ttcaaaaact ggcatggtga gccatttctt
     1441 ttctacacgg aaagggggag taagtagcgg tgtttttagt tcactgaatt ttgggtctca
     1501 gcatggagaa gcatcaaata tggtccggaa cattgattta ctgagggggg aagttgggtg
     1561 taaaacttct cggtttgtca tatcgaagca aactcatagc gatgttgtag ctgttgtcgg
     1621 ggttaataat ctcgacaact gttttgattc taccgatgcg ttaattacca acttgccaaa
     1681 tgttttcgtt tgtgtgaaaa ctgccgattg cgtgccaatt ttgcttttcg atcctgtatt
     1741 acgggttgta gcagcggtac attcgggctg gaggggtact gttcaaatat tgttggtaaa
     1801 tcaattgcgt taatggagaa agcttt
//
LOCUS       DAOPUW010000437         1823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_70685_length_1823_cov_2.090498, whole genome shotgun sequence.
ACCESSION   DAOPUW010000437 DAOPUW010000000
VERSION     DAOPUW010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70685_length_1823_cov_2.090498"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PKJ43_07420"
     CDS             <1..496
                     /locus_tag="PKJ43_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ72433.1"
                     /translation="DVAKYKESQRTNLRKGNIGFVFQSFNLIDELNVFENVELPLLYL
                     KIGTRERKEMVTKVLERMNIAHRAKHFPQQLSGGQQQRVAIARAVVANPKLILADEPT
                     GNLDSKNGLEVMNLLTELNREGTTIVMVTHSMHDAGFAHRIINLFDGQIITEEEKRKI
                     GDVL"
     gene            complement(502..834)
                     /locus_tag="PKJ43_07425"
     CDS             complement(502..834)
                     /locus_tag="PKJ43_07425"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HNZ72434.1"
                     /translation="MKLLFDQNLSPKLASHFRKHCNSSMHLLDVSLDFARDMMVWEYA
                     KSNDFTIVTKDSDFNNMVSLFGFPPKVVLIRRGNCSTKLIQTLIEKHLDEIRLFINDD
                     ENGIFTIL"
     gene            complement(834..1052)
                     /locus_tag="PKJ43_07430"
     CDS             complement(834..1052)
                     /locus_tag="PKJ43_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010424097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNZ72435.1"
                     /translation="MNYNDRITIEAGKRGGKPCVRGMRITVFEVLEYLASDMSMEEIL
                     DDFPYLEKEDILACLSFAANKEKIELCA"
     gene            1104..>1823
                     /locus_tag="PKJ43_07435"
     CDS             1104..>1823
                     /locus_tag="PKJ43_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ72436.1"
                     /translation="MNLINIRSTFNHLRKNKFHSLLNVFGLAVGLLFFLHIVLYIGYE
                     KGFDSFWPEKERLYRVNYDITQAGERVLHSTKTPRGLFRVIQEEVPEVEKAGIGYIEQ
                     VLVRYEDRYYSDQPDLWVEGDFAEVFGLEIKRGKATLNQPNTCVISESKAKEIFGNED
                     PIGKVLIVNEGMRHEITGIFEDLPANSHIHFHYFMPIRTWVNMGFLADRDGYEDAGWW
                     TYIKVKKGVKAEQAEKSLNSLV"
BASE COUNT          574 a          386 c          395 g          468 t
ORIGIN      
        1 cgacgtggcc aaatacaaag aaagtcagcg caccaaccta cgcaaaggca acattggttt
       61 cgtgtttcaa agcttcaacc tgattgacga actgaacgtg ttcgagaatg tggaattgcc
      121 gctactttac ctaaaaattg gcacccgcga acgcaaagaa atggtgacca aagtactgga
      181 acggatgaac attgcacacc gtgccaagca ctttccccag caattgtcgg gaggacagca
      241 acaacgtgtg gccatagccc gtgccgtggt tgccaatccg aaactgattc tggccgatga
      301 acccaccggt aacctcgact cgaaaaacgg tttggaagtg atgaacctgc tcacagaact
      361 caaccgcgag ggcaccacca ttgtaatggt aacccactcg atgcacgatg ccggctttgc
      421 acaccgaatc ataaatctat ttgatggaca aattattaca gaagaagaaa aacggaaaat
      481 tggggatgta ttgtaaaaaa atcaaagaat agtgaatata ccattctcat catcgtttat
      541 aaaaagacga atctcatcaa gatgtttttc gattaaggtt tgtatgagct ttgtagaaca
      601 atttcctcta cgaattagta caactttggg aggaaaccca aacagagaaa ccatgttgtt
      661 gaaatcagaa tcttttgtta ctatggtgaa atcattcgat ttggcatatt cccaaaccat
      721 catatccctg gcaaaatcaa gggatacgtc caataaatgc attgatgaat tacaatgctt
      781 tctgaaatgc gaagctaact ttggtgataa attttggtcg aaaagaagtt tcattaggca
      841 cagagttcaa tcttttcttt attggctgcg aaacttaaac aagcgagaat atcttctttc
      901 tcgagataag gaaaatcgtc aaggatttct tccatactca tatccgatgc aaggtattca
      961 aggacttcga aaacagttat gcgcatacca cgcacgcaag gttttccgcc ccgcttgcct
     1021 gcctcaatgg ttattctgtc attgtaattc atagcaaaat gatttctaac aaaaataaga
     1081 aataaaattt aaacactcgt tatatgaacc taatcaacat ccgttcaacc ttcaaccatt
     1141 tgaggaaaaa caagtttcat tcgctgctca acgtgtttgg tctggccgtg ggcttgctgt
     1201 tcttccttca cattgtactg tacattggct acgaaaaagg attcgactcg ttttggcctg
     1261 aaaaagaacg cctttacagg gtaaattacg acattaccca agcaggcgaa cgggtgttgc
     1321 actcaaccaa aacaccgcgc ggacttttca gggttatcca agaggaagta cctgaagttg
     1381 agaaagcagg gattggttat attgaacagg tgctggtgcg ctacgaagac cgctattaca
     1441 gcgatcagcc cgatttgtgg gtggaaggcg attttgccga ggtcttcggg ttggaaatta
     1501 aacgtggaaa agccacgttg aaccaaccca atacctgcgt gatttcggaa tcgaaagcca
     1561 aagaaatttt tgggaacgaa gaccctattg gcaaggtgtt gattgtaaac gaaggcatgc
     1621 gccacgaaat aactggtatt tttgaagatt tgccggccaa ctcgcacatt cattttcact
     1681 actttatgcc catccgcact tgggttaaca tgggtttctt agccgatcgc gatggttacg
     1741 aagatgcagg atggtggaca tacattaaag taaaaaaagg agttaaagcc gaacaggcag
     1801 aaaagtcgct caacagctta gta
//
LOCUS       DAOPUW010000438         1820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_70843_length_1820_cov_2.843059, whole genome shotgun sequence.
ACCESSION   DAOPUW010000438 DAOPUW010000000
VERSION     DAOPUW010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70843_length_1820_cov_2.843059"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PKJ43_07440"
     CDS             <1..358
                     /locus_tag="PKJ43_07440"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HNZ72437.1"
                     /translation="HQIRFGEKLTIEKKIEGDIREVQVPPLLFIPFIENVMKHCRTSP
                     NGNFFINMNFGVDGDKILFAIENSFDGNTHVIESGGIGLENVGKRLALLYPKKHYLTI
                     DKKENIFRVELFLNLS"
     gene            360..1043
                     /locus_tag="PKJ43_07445"
     CDS             360..1043
                     /locus_tag="PKJ43_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ72438.1"
                     /translation="MELKCIVADDEPLQQEILKDYIGMISGFELIGVFGNGNEALSFL
                     KEHKTDLVFLDISMPGLNGVELVKRLSNPPAVIFTTAYPEYAVEGFNLNAVDYLLKPI
                     SFERFSKAIEKFSHALGTGKDEIADTVLVKSGKKEYRIKLESIYYIESMGDYIRLVTA
                     TQAIITHGTMLQFLNILPANKFCRIHKSFTINLDLIDYFEGNIISLKGKKLSVGKAYR
                     EDFLETWRK"
     gene            1256..>1820
                     /locus_tag="PKJ43_07450"
     CDS             1256..>1820
                     /locus_tag="PKJ43_07450"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNZ72439.1"
                     /translation="MSLVGKKAPSFSSKAVVNGYEIVENFSLDQYVGNKYVIFFFYPM
                     DFTFVCPTEILAFQEKLTEFEKRGVAVVGCSTDSEFSHWAWLNTEKKNGGIKGVKFPI
                     VADLSKNIAMNYGVLAGEYDYDDQGNSIFNGEPVAFRGLFLIDKNGKVRHELINDLPL
                     GRSVDEAIRMVDALQHFEEVGEVCPANW"
BASE COUNT          547 a          296 c          402 g          575 t
ORIGIN      
        1 tcatcagatc cggtttggtg aaaagcttac tattgaaaag aagatcgaag gggatattcg
       61 tgaagttcag gtgcccccat tgctttttat tccctttatt gagaacgtga tgaaacattg
      121 tcgaacgtct cccaatggaa atttctttat taacatgaac tttggtgttg acggggacaa
      181 gattcttttt gcaattgaaa atagctttga tggaaacacc cacgtgattg aaagcggagg
      241 tataggattg gagaatgtag gcaaacgtct tgccttgctt taccctaaga aacattacct
      301 taccatcgat aaaaaagaga acatttttag ggttgaacta ttccttaatc tgagttgata
      361 tggagttgaa gtgcattgtt gccgacgatg aacccttgca gcaggaaatt ctgaaagatt
      421 atattggcat gatttccggc tttgagctta ttggcgtttt cggcaatgga aacgaagcat
      481 tgtctttttt gaaagagcat aaaaccgatc ttgttttcct cgatattagc atgcctgggt
      541 tgaatggagt tgaactggtt aaaaggcttt ccaatccacc ggctgttatt ttcacaactg
      601 cttaccctga atatgcggtc gagggtttca atctgaatgc tgttgattac ctgttgaagc
      661 ccatttcgtt tgagcggttt tctaaggcaa ttgagaaatt ctcacatgca ttgggaactg
      721 gtaaggatga aattgctgac accgttttgg ttaaatcagg gaaaaaggag taccggatta
      781 agcttgaaag catttattat attgaatcga tgggcgatta tataaggttg gtaacagcga
      841 cacaggcaat cataacccac ggaacaatgt tgcaatttct caatatatta cctgcaaata
      901 agttttgtcg tatacataaa tcgtttacca tcaatctaga cttgatcgat tactttgaag
      961 gaaacattat ttcgttgaag ggcaaaaaat tgtcggtagg aaaagcctac cgtgaggatt
     1021 ttctcgaaac atggagaaaa tagaagcttt taaccgcggc aaaaaattta atcttatctg
     1081 taataaatct aaaacctttt tctgatttcc attgtttact tttctgattg taacaaacga
     1141 ccaattctct ctattattgg gaaaaactta tggcgatatc tggttttaat gatgagggga
     1201 atggtttttt tgctcgatcg taatttcgat atcagataac attaaaacat aaaatatgag
     1261 tttagtaggg aaaaaagcac cttcatttag cagcaaggct gtagtaaatg gctatgaaat
     1321 tgtagaaaac ttttcattag atcagtatgt tggcaataag tatgttatat tcttttttta
     1381 tccaatggat tttacgttcg tatgcccaac tgaaattctt gcctttcagg aaaagttaac
     1441 tgaatttgaa aagaggggag tagcagtggt tggatgttcc accgattctg aattttcgca
     1501 ttgggcttgg ttaaatactg aaaagaaaaa cggaggcatt aagggggtaa aattccctat
     1561 tgttgccgat ttgtcgaaaa atattgccat gaattatggt gtattggccg gtgaatacga
     1621 ttacgatgac cagggaaatt cgatttttaa tggcgaaccg gttgctttca ggggcctctt
     1681 tcttatcgac aaaaacggta aagtacgcca cgagttgatc aacgatttgc ctcttggacg
     1741 tagtgttgat gaagccatcc gcatggtcga tgcacttcag cattttgaag aagtaggtga
     1801 agtatgcccg gcaaattgga
//
LOCUS       DAOPUW010000439         1815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_71131_length_1815_cov_3.698295, whole genome shotgun sequence.
ACCESSION   DAOPUW010000439 DAOPUW010000000
VERSION     DAOPUW010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71131_length_1815_cov_3.698295"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PKJ43_07455"
     CDS             complement(<1..674)
                     /locus_tag="PKJ43_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ72440.1"
                     /translation="MERKIEKYFASWKKSSGRMPLMVVGARQVGKTYSILSFGNSNYN
                     NVAYFNFESNPELQGIFQRNLNPIRIVQELSVLAGKTIVPEKTLVFFDEIQACEQALT
                     SLKYFNEEVNEYHIIAAGSLLGVAVNRNQYSYPVGKVEKINMYPLDFEEFLWALDQKS
                     GCTLIEEHFNANIEFSLHSKFIDLFNHYLTIGGMPQVVKEFIEKRDFNFVVALQKNIN
                     DAYVAD"
     gene            complement(858..1166)
                     /locus_tag="PKJ43_07460"
     CDS             complement(858..1166)
                     /locus_tag="PKJ43_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNZ72441.1"
                     /translation="MTPKELEERLIQFAIQIIKLSEGMNQSIAGKRLADQIVRSGSSP
                     ALNYGEAQSAESGKDFVHKLGVYLKELRETHINLRIIDGADLHPDKRPSLNLYSKVMN
                     "
     gene            complement(1204..1803)
                     /gene="hisH"
                     /locus_tag="PKJ43_07465"
     CDS             complement(1204..1803)
                     /gene="hisH"
                     /locus_tag="PKJ43_07465"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNZ72442.1"
                     /translation="MNLVIIKYNAGNIESVNNALMRLGVSAEITDDPEKIRAADKVIF
                     PGVGEASTTMAYLREHKLDELIRSLKQPVLGICLGQQLMCAHSEEGNVDCLGIFDEKV
                     KRFQPASGQEFVTKVPHMGWNNIYNLNSAILTPELENQFVYFVHSFYVEVGEHTAATC
                     DYINPFSAALHKDNFYATQFHPEKSQKVGARILENFLKI"
BASE COUNT          510 a          368 c          340 g          597 t
ORIGIN      
        1 atatccgcta cgtatgcatc gtttatattt ttttgcaggg caacaacaaa gttgaaatcc
       61 cgcttctcga taaactcttt aaccacctga ggcatgccgc caatggtcaa ataatggttg
      121 aacaaatcaa taaattttga atggagtgag aattcaatat tcgcattaaa atgttcctca
      181 attagtgtac aacctgattt ctgatccaac gcccaaagaa actcctcaaa atcgagcggg
      241 tacatgttta ttttctcaac cttcccaacc ggatacgaat attgatttcg attaacggcg
      301 acacccaata aacttccagc tgcaattatg tgatattcat tgacttcttc attaaagtat
      361 ttcaacgaag taagtgcttg ctcacaagct tgtatctcat cgaaaaacac taaggttttt
      421 tccggaacaa tggtttttcc tgccagtacc gaaagctctt gcacaatacg gatcggattc
      481 aaattgcgtt gaaaaatacc ttgcaattca ggattgcttt caaaattgaa atatgccaca
      541 ttgttatagt tgctgttacc aaaagaaagt atggaatatg ttttcccaac ctgtcttgca
      601 ccaacaacca tcaacggcat tcttcccgat gattttttcc agctagcaaa atatttctca
      661 atcttacgtt ccatttagtg cgtttaaatt gcatttatag gtttcaatta gtgtaaattt
      721 acaacacaaa attgaatatt tgtgaattaa gattaacgat taacgatttt tgattaacaa
      781 ttaacgattt gattgtggat tcgaaatttg attttgtttg cgtttactgg tttcaatgct
      841 tcttacaaag attgaaatca attcattact ttcgagtata agttcagtga tggtctttta
      901 tcaggatgaa gatctgctcc atcaataatt cggagattga tatgtgtttc acgaagttct
      961 tttaagtaga cacccaactt gtgaacgaaa tccttacccg attcagcact ttgtgcttca
     1021 ccataattta atgcaggtga tgaaccagaa cgtacaattt gatcagccaa tctttttcca
     1081 gcaattgatt gattcatgcc ttcggaaagc ttaattattt gaatggcaaa ttgaattaat
     1141 cgttcttcta attctttagg tgtcataact acttcgttaa tcgttaatcc tcaatcgtta
     1201 atttcaaatc ttcaaaaagt tttccaatat ccgtgcgccc accttttggc ttttttcggg
     1261 gtggaactgc gttgcgtaaa agttgtcttt atgcaatgct gcgctaaatg ggttgatgta
     1321 gtcacacgtt gctgctgtgt gttcgcctac ttcaacatag aacgagtgaa caaagtatac
     1381 aaactggttc tccaattcgg gtgtgagaat ggcactgttc aggttgtaga tgttgttcca
     1441 gcccatgtgg ggtactttgg taacaaattc ttgccctgaa gcgggttgaa agcgtttcac
     1501 cttttcgtcg aaaatgccga ggcaatccac attgccttct tcactgtggg cgcacataag
     1561 ctgctgtccg aggcaaatac ccaaaacggg ttgtttcaga ctgcggatca gctcgtcgag
     1621 tttgtgctcg cgcaggtagg ccatggtagt actggcttcg cccacgccag ggaagatcac
     1681 tttatcggca gcgcgaattt tctcgggatc gtcagtaatt tcagctgata cacctagtcg
     1741 catcaaagca ttgttcaccg attcaatgtt cccggcattg tatttaatga ttacaaggtt
     1801 catgttttat ttttt
//
LOCUS       DAOPUW010000440         1813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_71248_length_1813_cov_3.980660, whole genome shotgun sequence.
ACCESSION   DAOPUW010000440 DAOPUW010000000
VERSION     DAOPUW010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71248_length_1813_cov_3.980660"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            483..>1813
                     /locus_tag="PKJ43_07470"
     CDS             483..>1813
                     /locus_tag="PKJ43_07470"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNZ72443.1"
                     /translation="MSTKYIFVTGGVTSSLGKGILSASLAKLLQARGYSVTIQKLDPY
                     INVDPGTLNPYEHGECYVTEDGAETDLDLGHYERFLNIPTSQANNITTGRIYQTVINK
                     ERRGDYLGKTVQIIPHITDEIKRQIKFLGNKGKFDFVITEIGGTVGDIESLPYIESVR
                     QLKWELGRSAMVIHLTLVPFLSASGELKTKPTQHSVKALLENGVQPDILVLRTEHNLN
                     DGLKKKVALFCNVAEEAVVQSIDVPTIYEVPLKMKEEKLDITVLKKFGMTSSKEPALD
                     DWNHFLQRLNNPKQEIEIGLVGKYVELHDAYKSIAESFIHAGAVNECKVRLRYIHSEE
                     ITKRNVAEMLSGLQGVLIAPGFGHRGIHGKIEATRYARENNIPFLGICLGLQIAVIEF
                     ARNVLGLQGADSTEMNEVTKYPVIDLMEEQKSVTNMGGTMRLGAYACEVKKG"
BASE COUNT          613 a          358 c          341 g          501 t
ORIGIN      
        1 aatacttaaa tatttattct gggtaacatg actctggaaa tttccaaaaa ggaaaagcga
       61 taaatcgata ccctccgaca gatctacttc ataatgaatg ccatggcggg ttatctttct
      121 gattgttcta cgataaaaca atttcactat tttgtacagc attttcgcta taccaatctt
      181 aattctggtg ataggtaaac ccaacatgtt acgtttaatt ttgttagaca cgcaaatgta
      241 taaattaata cttgccgaag gagattatcc atcataaaac aactaaagac caaccccaat
      301 gtacaaataa cagaacaaaa aacaatgact atttgaaagc taaaataatg ttttcttgta
      361 catttttata gtaaaggtta attctatttc aaatatagta tcttttatct gaaattaata
      421 atacaattta tttttcacta tctttgctct ttctttacat taacccataa aaattttaca
      481 ttttgtcgac taagtatatt ttcgttaccg gaggtgttac atcctcatta ggaaaaggga
      541 ttttatcggc ctctttggct aaacttcttc aggcccgtgg ctattctgtg accatacaaa
      601 aactcgatcc atacatcaat gtcgatcccg gaacattaaa cccatatgaa cacggtgaat
      661 gctatgtaac tgaagacgga gctgaaaccg accttgacct tggacattat gagcgatttc
      721 ttaacatacc aacctcacaa gccaataaca ttacaaccgg cagaatatac caaaccgtta
      781 taaataaaga gcgacgcggt gactaccttg gaaaaacagt tcagataatc ccacatatta
      841 ccgacgaaat caagcggcaa ataaaatttc tcggaaataa aggaaaattt gattttgtaa
      901 taactgaaat cggaggaaca gtgggcgata tagagtcgtt accttacatt gaatcggtac
      961 gccagctcaa atgggaactt ggacgttcag ctatggtaat acacctaacc ttggttcctt
     1021 tcttatcggc atcgggagaa ttgaaaacca aacccacaca acattcggtt aaagctctgc
     1081 tcgaaaacgg agttcaaccc gatattctgg tgctacgcac cgaacacaat ttaaatgatg
     1141 gactcaagaa aaaagttgct cttttttgca atgttgctga agaagctgta gtacaatcaa
     1201 ttgatgtacc aacaatttac gaggttccat taaaaatgaa agaagaaaaa ctcgatatta
     1261 ccgtattgaa aaaatttggt atgaccagca gcaaagaacc agcacttgat gattggaacc
     1321 attttttaca acggctgaac aacccgaaac aagaaataga aattgggctg gttggtaaat
     1381 atgttgagct tcacgatgcc tacaaatcta ttgctgagtc gtttatccat gctggagcag
     1441 taaacgaatg caaagtaagg ctccggtata tccattctga agaaattacc aaacgtaatg
     1501 ttgccgaaat gctctcgggg ttgcaagggg tgctgatagc tccaggcttt ggtcacagag
     1561 gaattcatgg taaaattgaa gcaacccgtt atgccaggga aaacaatatc ccttttcttg
     1621 gaatctgcct tggactccaa atcgcagtta tcgagttcgc ccgcaatgtg ttgggcttgc
     1681 agggagccga ttccactgag atgaacgagg ttacaaaata cccggtaatc gatcttatgg
     1741 aagaacaaaa aagcgtaacc aacatgggcg gtaccatgcg attgggggct tacgcctgcg
     1801 aagtaaaaaa agg
//
LOCUS       DAOPUW010000441         1811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_71399_length_1811_cov_3.010251, whole genome shotgun sequence.
ACCESSION   DAOPUW010000441 DAOPUW010000000
VERSION     DAOPUW010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71399_length_1811_cov_3.010251"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..1072)
                     /locus_tag="PKJ43_07475"
     CDS             complement(200..1072)
                     /locus_tag="PKJ43_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HNZ72444.1"
                     /translation="MTIEATHLGKQSSYPQHYDPSVLVAVPRILNREQYGISNNALPF
                     SGVDVWHAYEFSFLTAKGAPVVGLLKMVYSAGNEFLVESKSLKLYLNSFNMNRFGETR
                     AEGISAVTQIIGKDLSELLKCKVQVAYFDQNAQPSNSDFNDFSILEETADFDNISCTI
                     YTETPDLLQKSQTSGEIKWATHLLRSNCKITHQPDWGSLFIEMNGKQLPTPESFLKYI
                     VSLRNENHFHEEICEMVFKRLIDVYAPEKLMVACMYTRRGGIDINPVRVLPGTELPAT
                     LVNPTLRTNLSFRQ"
     gene            complement(1082..1471)
                     /locus_tag="PKJ43_07480"
     CDS             complement(1082..1471)
                     /locus_tag="PKJ43_07480"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNZ72445.1"
                     /translation="MTGTTSDYIKYRIEKADDSFSDAKLLFSNQRWNACVNRLYYSCF
                     YITIALLVKNDIETTTHDGVRNQLGKVFVKTGIIEKQLGKLYSKLFDYRLKGDYGDWF
                     DFDEKLVTPLIPEVEKYLSRLKELIDL"
     gene            complement(1468..1785)
                     /locus_tag="PKJ43_07485"
     CDS             complement(1468..1785)
                     /locus_tag="PKJ43_07485"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNZ72446.1"
                     /translation="MGKKEMINKIRAAILSKDQHAQSFLFGSRARNTNRNNSDWDILI
                     LVNQPNISNEIEDHFRDALYDIELQSGQTISTIIYPKQIWFEKMKSTPLFNAIKNEGI
                     ALT"
BASE COUNT          514 a          339 c          362 g          596 t
ORIGIN      
        1 acagtttgca aataatctaa aaaataattt ttgtttaaaa ttaataaaaa caccataatc
       61 tttaattatt caattatacc tcatctaaaa ctttcaggaa ttgcgatata cattttttgc
      121 tgtaaaagtt aagcgtactg ttaacttaag aaaaataaca tgagagtggt ttgaattttt
      181 ttggtgcttc ctacttgttt tattgcctga acgaaaggtt ggttcgtagg gttggattta
      241 ccaatgtggc aggcagttca gtgccgggta aaactcgcac cgggttaatg tcgatgccac
      301 cacgacgggt gtacatgcag gccaccatga gtttttcggg ggcgtaaaca tcaatcagcc
      361 gtttaaacac catttcgcaa atctcttcgt gaaagtggtt ttcgtttcgc agcgagacaa
      421 tgtatttgag gaagctttcg ggcgtaggca actgtttgcc attcatttca ataaacagcg
      481 aaccccagtc gggctggtgg gtaattttgc aattgctccg aagcagatgt gttgcccatt
      541 ttatttcacc actggtttga cttttttgca gcaaatcagg tgtttcggtg taaatggtgc
      601 atgatatatt gtcgaaatcg gctgtttctt caagaatcga aaaatcatta aaatcagagt
      661 tggaaggttg ggcattttgg tcaaagtaag ccacctgaac cttgcatttc agcagttcgg
      721 aaaggtcttt cccaatgatt tgggtaacgg ccgaaatccc ttctgcgcgg gtttctccaa
      781 accggttcat gttgaacgag ttgaggtaga gcttcagcga cttgctctcg acaaggaatt
      841 cgttgccggc cgagtaaacc atctttagca aaccaacaac cggagctcct ttggctgtga
      901 ggaaactgaa ttcgtaggca tgccacacat cgacacccga aaagggcaac gcgttgttcg
      961 aaatcccgta ttgctcacgg ttcagaatgc gcgggacagc aaccagaaca cttgggtcgt
     1021 agtgctgcgg gtaactgctt tgttttccta aatgagttgc ttcaatggtc atcgttactt
     1081 gctataaatc tatcaactct tttagccttg ataagtattt ctcaacttca ggaataagtg
     1141 gagtaactag tttttcatca aaatcaaacc aatcaccgta atctcccttt aagcggtagt
     1201 cgaaaagttt tgagtacaat ttcccaagtt gtttctcaat tatccctgtt tttacaaaaa
     1261 cttttcctag ttgatttcta acgccatcgt gtgttgtagt ttcaatatcg ttttttacta
     1321 acaatgctat agttatgtag aagcatgagt aataaagccg attaacacat gcattccatc
     1381 gttgatttga gaagagaagt tttgcatctg agaacgaatc atctgctttt tcaattcgat
     1441 atttaatata atcagaggtg gttcctgtca tgttaatgct attccttcgt ttttaatggc
     1501 attaaaaagc ggagttgatt tcattttctc aaaccaaatt tgttttggat aaatgattgt
     1561 agaaattgtt tgacctgatt gtaattcaat atcataaagt gcatcacgaa aatgatcttc
     1621 aatttcgttg gatatatttg gttgattaac caaaatgagg atatcccagt cggaattatt
     1681 tctatttgta tttcgagctc tcgaaccaaa taaaaaagac tgtgcatgtt gatctttcga
     1741 taaaattgca gctctaattt tatttatcat ttccttttta cccatgactc ggctttataa
     1801 attcaaaggt a
//
LOCUS       DAOPUW010000442         1811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_71405_length_1811_cov_2.848519, whole genome shotgun sequence.
ACCESSION   DAOPUW010000442 DAOPUW010000000
VERSION     DAOPUW010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71405_length_1811_cov_2.848519"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            730..>1811
                     /locus_tag="PKJ43_07490"
     CDS             730..>1811
                     /locus_tag="PKJ43_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNZ72447.1"
                     /translation="MLFFQSSSISYKKIWNIAYPIIIGSVAQNIINVTDTAFLGRLGE
                     IALGGGAIGGLFYIAFLMFGWGFGVGTQIVVARRHGEGVYRPIGRVVEHGFLFLMALA
                     TMLFFLARVFGTQILSYIVKSEAVSQASAQYLGYMIFGIFFAHINFIFRAFYVGIGNT
                     RVITVTTLVMVLINVFLDYALIFGNFGFPSMGIAGAAIASVVAEISSTLAFLAYTLMK
                     VSVKQYKLFSFNVISPKLLLRLLRISLPIMLQNFLGFGVWFLFFLIIEKMGETQLAVS
                     NIIRSVYIVLSVPIMGFASATNTLVSYVIGQGRQHEVLATIGKIAVICVSSVALVVGI
                     CATMPRHIISIYTSDPVLIQMGVPIL"
BASE COUNT          461 a          337 c          389 g          624 t
ORIGIN      
        1 gacgaagttg taccctgttc cttcggacga caggatatta acaataggaa aagtaagacg
       61 aaaaacgtga tattagtatt tataacccaa tatcttactt tgaagcgggg cattatttca
      121 tctttttgca tattccatgc aagaaataaa aaaatactga caattcaaag agttaaaatt
      181 ttttgctttc aacattatgt aagcactaca gctttagctc caacctactc ttttgtaaag
      241 gtagtaaaaa tctgttttaa taagatgcac attttgcgaa taattctcaa cacgttttca
      301 atattttacg aaattatggt tgcggttttg cgaattcagg tcttaaaaaa tcaatttatc
      361 attgctaagt atctgactgt taagagtatt tcttgttctc aaaatgctat gtttaactta
      421 agaaatgggg aaaatgcttt caatgccttt gtatgtgatt atttttacga gtacaatgtg
      481 ttgatattta gatgcctggg attatttttt tattcgatac gtgctgtttg tatagcaaat
      541 gggttgtcaa taaaaaatag ccattgcccg tgcagaatca acctttactc atactctctg
      601 ttactttgtt gtacttttgc agcttcgctg tggcttcttt caagcattgg ttgctgacgt
      661 ttcgtctatt ggtgaacaat gctatttgtc cgaaaggata taaggtaatg ggtttgtctt
      721 taaattgtta tgttgttttt tcaaagttca tccatatcat ataaaaaaat ctggaacatt
      781 gcctatccca ttatcattgg cagtgtagcc cagaacataa taaacgtaac cgatactgcc
      841 tttttagggc ggttgggcga gattgccctt ggcggtggag ccattggagg ccttttctac
      901 atagcctttt taatgtttgg ctggggattc ggggttggga cacagattgt tgtagcccgt
      961 aggcatggcg aaggggttta tcgccctata gggcgggtgg tggagcatgg tttccttttc
     1021 ctcatggcgt tggccacgat gcttttcttt ctcgccaggg tctttggcac tcaaatacta
     1081 tcgtacatag taaaatcgga agcggttagc caggccagtg cccaatacct gggctatatg
     1141 atttttggaa ttttctttgc gcacatcaac ttcattttca gagcatttta tgttgggatt
     1201 ggcaacacaa gggtgattac ggttacaaca ttggttatgg tgttaatcaa tgttttcctg
     1261 gactatgcgc ttattttcgg aaattttggc tttccttcca tggggatagc aggggcagcc
     1321 atagcatcgg tggtagccga gatttcgagc actctggctt ttcttgccta taccctgatg
     1381 aaagtttccg tgaaacaata caagttattt agttttaatg tgatcagccc taaactgctg
     1441 ttgcgcttac tcaggatttc tctccccatt atgctccaaa actttttagg atttggcgtt
     1501 tggtttttat tttttctgat tattgaaaag atgggcgaaa cgcagttggc tgtttccaac
     1561 atcattcgct ccgtttatat cgtgttaagc gttcccatca tgggttttgc ttcggccacc
     1621 aatacgcttg tcagctatgt aatagggcag ggacggcagc atgaagtatt ggcaaccatt
     1681 ggcaagatag ccgttatctg tgtatcatct gtcgcactgg tggtgggtat atgcgccacc
     1741 atgccccgac atattatttc aatttacact tccgacccgg tgctaataca aatgggtgtg
     1801 ccaattttgt a
//
LOCUS       DAOPUW010000443         1810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_71453_length_1810_cov_3.151567, whole genome shotgun sequence.
ACCESSION   DAOPUW010000443 DAOPUW010000000
VERSION     DAOPUW010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71453_length_1810_cov_3.151567"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..>1810
                     /locus_tag="PKJ43_07495"
     CDS             112..>1810
                     /locus_tag="PKJ43_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNZ72448.1"
                     /translation="MKNELKVWEWTTSYANKFCSVFLTTVLSLVFSVCFVFPAFSADD
                     KGSEMSNESNSNQLQQSKSITGKVVDEDGVALPGVSVVVKGSTNGTITDIDGKYTLSV
                     PSSAKTLVFSFIGMTPKEVLIGTQSQIDVTLESGIINLEEVVAIGYGSMEKNNVTGAI
                     SSIKTEEIMKAPVPNVVEALRGQVSGVKISKGSGRPGSGVDLLIRGKKSLSSSNEPLI
                     VIDGVPNTGGNLAEINPADIASINILKDAAAASIYGASAANGVVLITTKDGVFGRPSV
                     NVEFSYGISDLVMEPEMFNAREYVAVKNAASVGAGLGEKTPEQLLDDVELVNWGNGDS
                     IKETNWHDKLLKIGDIKNASVSLSGGTEKFHYYMNGDAYLEDGIAQYSTYNRYSMRLN
                     ADYAPYKFLTIGARVQLSKSIADETGSTLYQGNADFGDFVGNSPLGRLYDEYDRLVPT
                     VKGDQFQYNPLYRYRESKVDRNSSRVYINPWIELKIIDGLTYRMNAFAEQRFENYKEF
                     YSSIYNNSVLDADPGTNQIRIQLKEDLTYLWDNILNYRRIFNDVHSVDATLVYGIQTY
                     "
BASE COUNT          542 a          308 c          401 g          559 t
ORIGIN      
        1 ggatagcttt ttttgatcgg ggttgttaaa atgttgaatg tgggaatttt attttatgca
       61 accgatgatg taaattttga tgaataaata tattatctaa ctaatcaatc tatgaaaaat
      121 gaattaaaag tttgggagtg gaccacttcc tatgcaaaca aattctgttc ggtttttctt
      181 acaaccgtgc tttcattagt cttttcggta tgctttgtct ttccagcctt ttctgctgat
      241 gacaaaggat cggaaatgag caatgaatca aattcgaacc aattgcaaca atcaaagtcc
      301 attacaggta aagttgttga tgaagacgga gttgccctgc ctggtgtttc agttgttgta
      361 aaaggatcta cgaatggaac gattaccgat attgatggta aatacacctt gtcggtgcct
      421 tcttcagcta aaacgctagt tttttctttc attgggatga cgcccaaaga agttttaatt
      481 ggaacacaat ctcaaattga tgtcacgctt gagtctggga ttattaattt ggaagaagtt
      541 gttgcaattg ggtatggatc aatggagaaa aataatgtaa ctggtgcaat ttcttcaata
      601 aaaaccgaag aaattatgaa agcccctgtg cccaatgttg ttgaagctct cagaggtcag
      661 gtttctgggg ttaaaatatc aaaaggaagt gggcgtccag gttcaggggt tgatcttctt
      721 attcgtggta agaaatcgtt gagttctagc aatgagccac ttattgttat tgatggtgtg
      781 ccaaacactg gcgggaacct tgctgaaatt aatcctgccg atatcgcttc gattaacatt
      841 cttaaagatg ccgcagctgc ttcaatttat ggtgccagtg ctgcaaatgg ggtagttctt
      901 attacaacaa aagatggtgt tttcggacgg ccttctgtta atgttgaatt ctcgtatggg
      961 ataagtgacc ttgtcatgga acccgaaatg tttaatgccc gggaatatgt ggccgttaaa
     1021 aatgcagctt cggttggtgc agggttggga gaaaaaactc ctgaacaatt gctcgacgat
     1081 gttgaactgg taaactgggg gaatggcgat tcgataaaag aaacaaattg gcatgataag
     1141 ctgcttaaaa ttggcgatat taaaaatgca agcgtttctc tgtcgggcgg aactgaaaag
     1201 tttcattatt atatgaatgg cgacgcatac cttgaagatg gcattgccca gtattcaacg
     1261 tacaatcgtt attcaatgag gcttaatgcc gactatgccc cctataaatt tctgacaatt
     1321 ggagccaggg ttcagctttc aaaaagtatt gcggacgaaa caggcagtac tttatatcag
     1381 gggaatgccg actttggaga cttcgttggc aactcgccac ttggacgttt atatgatgaa
     1441 tatgaccgtt tagtgccaac cgttaaagga gaccagtttc agtataatcc gctctatcga
     1501 tatcgcgaat ctaaggtcga tagaaactca tcacgagtat acattaaccc atggattgaa
     1561 ttgaaaatta ttgatggatt aacgtatcgc atgaatgctt ttgctgagca acgttttgaa
     1621 aattacaaag aattttattc gtcgatctat aacaactctg ttcttgatgc tgatccaggg
     1681 acaaaccaaa tccgtattca gttaaaagaa gatcttacgt atttgtggga taatatctta
     1741 aactatcgta gaatatttaa cgatgtacat tctgtcgatg ccacactggt atatggaatt
     1801 caaacataca
//
LOCUS       DAOPUW010000444         1806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_71709_length_1806_cov_2.986294, whole genome shotgun sequence.
ACCESSION   DAOPUW010000444 DAOPUW010000000
VERSION     DAOPUW010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71709_length_1806_cov_2.986294"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PKJ43_07500"
     CDS             <1..845
                     /locus_tag="PKJ43_07500"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ72449.1"
                     /translation="IRVWGTIGFIAAMWIVDLMGLKEGPGQFYLSASASLLLGLYAFF
                     MPPCKPDNSQRGKSVVSALGLDAIVLFKNPKMAIFFVFSMLLGAALQITNTFGQPFLM
                     DFKTDYATSFAVTHPGILMSISQISETLFILTIPFFMKRFGIKNVMIMSMVAWVLRFG
                     LFGLGNPGSGFILLILSMIIYGMAFDFFNISGSLFVEMETTKKIRSSAQGLFMIMTNG
                     IGTIIGGLVSGLVVDHFTIDGIKNWPGIWFAFAAYALLLSIIFPLLFRYKHQPEKIKS
                     RSAQ"
     gene            860..1654
                     /locus_tag="PKJ43_07505"
     CDS             860..1654
                     /locus_tag="PKJ43_07505"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNZ72450.1"
                     /translation="MKQYHDLLKHILENGVRKDDRTGTGTISTFGYQMRFNLQEGFPV
                     VTTKKLHLKSIIYELLWFLNGDTNAKYLQDNGVRIWNEWADEDGNLGHIYGFQWRSWP
                     DYNGGSVDQITEAVNTIKNNPDSRRIIVSAWNVADLPNMNLPPCHAFFQFYVANGKLS
                     CQLYQRSADSFLGVPFNIASYALLTMMIAQVCGLEPGDFVHTFGDLHIYTNHIEQVKL
                     QLSREPYPLPQMKINPAVKSIFDFKYEDFELVNYQSHPHIKGEVAV"
BASE COUNT          509 a          374 c          383 g          540 t
ORIGIN      
        1 caataagagt ttggggtacc attggtttta ttgccgccat gtggattgtt gatttaatgg
       61 gattaaaaga aggcccggga caattctacc tcagcgcttc agcatcgctg ttgcttggcc
      121 tgtatgcctt ttttatgcct ccttgtaaac cggacaattc gcaacgcgga aaatcggtag
      181 tatctgctct tggactcgat gccattgtgc ttttcaaaaa tcctaaaatg gctattttct
      241 tcgtattctc tatgttactg ggtgcagcat tgcaaataac caacaccttt ggacagccat
      301 ttcttatgga ttttaaaacc gattatgcca cgtcttttgc cgttacacac ccgggtatac
      361 tcatgtcgat ttcacagatt tcagaaaccc tgttcatcct taccatacca ttttttatga
      421 aaagattcgg aatcaaaaac gttatgataa tgagtatggt tgcctgggtt ctccgtttcg
      481 ggctgttcgg tcttggtaat cccggcagtg gtttcatctt gctcattctt tcgatgatca
      541 tctatggtat ggcttttgac tttttcaata tatcgggttc gttatttgtt gaaatggaaa
      601 caacaaaaaa aatccgctca agtgcacaag gcttgtttat gattatgacc aacgggatcg
      661 gaacaataat tgggggacta gttagcggat tggttgtaga ccattttaca atcgacggaa
      721 ttaaaaactg gccaggcatt tggtttgcat ttgctgcgta tgcattattg ctcagtatta
      781 tattcccact acttttccgc tataaacatc aacccgaaaa aatcaaaagc agatcggcgc
      841 aataagaaca agaatttgaa tgaaacaata ccacgattta ctaaagcata ttctcgaaaa
      901 tggtgtgcgc aaagatgacc gcaccggaac gggaaccatt tcgacatttg gctatcaaat
      961 gcgcttcaac ttgcaagaag gttttcctgt agtaactaca aaaaagttac acctgaaatc
     1021 tattatttac gagttgctat ggttcttgaa cggcgacacc aatgcaaaat atttgcagga
     1081 taatggtgtg cgcatctgga acgaatgggc agatgaagat ggaaatcttg ggcatattta
     1141 cggtttccaa tggcgctcgt ggcccgatta caatggtggc tcggtcgatc agattaccga
     1201 agcggtgaac accattaaga ataatcccga ctcgcgtcgc attattgtaa gcgcatggaa
     1261 tgttgccgat ttacccaaca tgaacttgcc tccctgtcac gcatttttcc aattttatgt
     1321 agccaatggg aagttatcgt gccagttgta ccaacgcagt gccgattcat ttttgggcgt
     1381 tccgttcaac atcgcttctt acgcactgct taccatgatg atagcacagg tttgcggtct
     1441 cgagcccggc gattttgtgc acaccttcgg cgacttgcat atttacacca accatataga
     1501 gcaggtgaaa ctacagctga gccgcgaacc gtatccgctt ccacaaatga aaatcaaccc
     1561 agcagtgaaa agcattttcg atttcaaata cgaagatttc gagctggtga actatcagtc
     1621 gcacccgcat attaagggcg aagtggcggt gtaaaagttg attgattatg aatatacaaa
     1681 aattagccaa tattattgtt gaaattcatg acaagatgca gcaccaagca atatctgctg
     1741 ttaatgtata tttgacaata cgtaattggt tgattggtta ttatatattg gaatttgagc
     1801 aaaacg
//
LOCUS       DAOPUW010000445         1805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_71758_length_1805_cov_3.266857, whole genome shotgun sequence.
ACCESSION   DAOPUW010000445 DAOPUW010000000
VERSION     DAOPUW010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71758_length_1805_cov_3.266857"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PKJ43_07510"
     CDS             complement(<1..766)
                     /locus_tag="PKJ43_07510"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNZ72451.1"
                     /translation="MNSTEFSEYKIKRISEVARELGIDPERILPHGHYIAKVPVSILD
                     EKKDQPDGKLILVSAMTPTPQGEGKTTTTIGLGDALRKKGLKTTICLREPSLGPYFGI
                     KGGGTGAGRAQVMPSEDINLHFVGDMYSVSKANNLLAAMVDNHLQHGNELNIDPRRII
                     WRRVIDLNDRALRQIFIGMGGVSNGVPRQDGFNITPASEIMAILCLASDVHDLGERLA
                     RTCVGFTYSGQPVRASDLQAEGAMQILLRDTIHPNLV"
     gene            1026..>1805
                     /locus_tag="PKJ43_07515"
     CDS             1026..>1805
                     /locus_tag="PKJ43_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ72452.1"
                     /translation="MLKRIPFQYRITVLYIFFGALWILFSDQIMVALTDDTHKIHLLS
                     TYKGWLFVLVTGILLYYLVRGEIRRRTLIFSELLEAKRKADESDRLKTTFLANLSHYV
                     RTPMNGILGFVQLLEDKDLSLEKQKLFHSYINELSQHLLRTLNSIIEISKIQTGQLQL
                     SPERVNINEYVEKIASGLSFDLQNKNKPVEVLTTITGDPASQYYLFDKDKVAEVFSNL
                     LSNAVNFTHIGHIEIGCLKNRSELVFWVRDTGKGIPKEKADT"
BASE COUNT          488 a          369 c          373 g          575 t
ORIGIN      
        1 gtaccagatt ggggtgaatg gtgtcgcgca aaagaatctg catggcgcct tcggcttgca
       61 aatcgcttgc ccgaacaggt tgtccgctat acgtaaaacc aacacacgtg cgggcaagtc
      121 gctcgcccaa gtcgtgaaca tcgctggcaa ggcacaaaat ggccataatt tctgaagcag
      181 gtgttatgtt gaaaccatct tgccggggta caccgttcga tacaccgccc atgccaatga
      241 agatttgacg caacgccctg tcattaaggt cgattaccct tcgccagatt atccgtcttg
      301 ggtcaatgtt caattcgttg ccatgttgaa ggtgattatc gaccatggcg gccagcaggt
      361 tgttggcttt gctcacagag tacatatcgc ctacaaaatg gaggttaata tcttctgagg
      421 gcataacttg tgcccgtccg gccccagttc ctcctccttt aattccaaag tatgggccta
      481 acgatggttc tctcaggcag atagttgttt ttagaccttt ttttcgcagt gcatcgccca
      541 gaccaatggt cgttgttgtt tttccttcgc cttgaggggt tggggtcatg gcacttacca
      601 ggatcagttt tccgtctggt tggtctttct tttcatccaa aatgctcaca ggaaccttgg
      661 ctatgtaatg cccatgtggc aatattcgtt ctgggtcgat tcctaattcc ctggcgactt
      721 cgcttattct tttgatcttg tattcgctaa attctgtact gttcatattg ataggtttta
      781 tgttcttcac ttttatattt ttgcttacca ttcgaaaaat aacactcttt cttatattga
      841 gcaacaaaag aataggtagg ttttattttg tcgtattctt tttttaaaaa gtatgatttt
      901 cctttatgtt atgtattgcc tccgaacaaa gattatacaa agaagcttaa ccattttatg
      961 ttaatcggag tattgcgctt tacaaaaaaa acagtaaatt taaaaacgta aaacccgaag
     1021 acataatgct aaagagaatt cctttccaat atcgaatcac tgtattgtac atcttctttg
     1081 gtgcactttg gattcttttt tctgaccaaa taatggtagc actaaccgac gatactcata
     1141 aaattcatct tctttctact tataaagggt ggctttttgt gttggttacc ggcattttgc
     1201 tctattacct tgttcgtggc gagataaggc ggcgaacgct tattttttcc gaactacttg
     1261 aggctaaacg aaaagccgat gagtcggatc ggcttaaaac tacttttttg gcgaaccttt
     1321 ctcattatgt gcgtacgccc atgaatggca tacttgggtt tgttcaattg ctcgaagaca
     1381 aagatttaag ccttgaaaag caaaagttat ttcattcata tattaatgag ctgagtcagc
     1441 atttgttacg caccttaaat agcattatcg aaatatcgaa aattcaaacc gggcaattgc
     1501 agctttcacc ggagagagtg aatataaatg aatatgttga aaaaattgct tcggggctta
     1561 gcttcgatct tcagaacaaa aacaagccag ttgaggtgct aacaacaatt acaggtgatc
     1621 cagcatccca gtattatctt tttgataaag acaaggttgc agaagtgttc tcgaacttat
     1681 tgtcgaatgc agttaatttt acgcatatcg ggcatattga aataggatgt ttgaaaaacc
     1741 gctcagaatt agttttctgg gttcgcgata ccggtaaagg aattcctaaa gaaaaggccg
     1801 atacg
//
LOCUS       DAOPUW010000446         1804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_71822_length_1804_cov_3.403659, whole genome shotgun sequence.
ACCESSION   DAOPUW010000446 DAOPUW010000000
VERSION     DAOPUW010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71822_length_1804_cov_3.403659"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1519
                     /locus_tag="PKJ43_07520"
     CDS             <1..1519
                     /locus_tag="PKJ43_07520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNZ72453.1"
                     /translation="GILSRNYDGLQNDLDKMDGVFLKRGDYTYALNDDKLWDFKPLAK
                     PGEKVIAGSWLGEVDENSKSHKIMVPFAFSGEYTVKSVIAAGQYKITDTVAVLINSDG
                     EEIAVTMIQRWPVKRPITAYKEKPRPFKLLETGVRTIDTINPIVEGGTGFIPGPFGTG
                     KTVLQHAISKQAEADIVVIAACGERANEVVEIFTEFPELEDPHTGRKLMERTIIIANT
                     SNMPVAAREASVYTAMTLAEYYRSMGLKVLMMADSTSRWAQALREMSNRLEELPGQDA
                     FPMDLSAIIANFYARAGFVYLNNGATGSVTFIGTVSPAGGNLKEPVTESTKKAARCFY
                     ALQQSRADSKRYPAINPIDSYSKYLEYPEFEEYIAKRIGANWIEMVNEAKTILLRGQE
                     VSEQINILGDDGVPLDYHIRFWKSEVIDFVVLQQDAFDKVDMLTPLERQQYMLKKVME
                     ICHADFNFDSFNEVENYFKRIINQYKQMNYSEFDSAQFKKYEEELASIINERKIA"
     gene            1519..>1804
                     /locus_tag="PKJ43_07525"
     CDS             1519..>1804
                     /locus_tag="PKJ43_07525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665150.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNZ72454.1"
                     /translation="MATKAFQKIYTKITKITKATCTLNAQGIGNEELAEIDGRLAQVV
                     KIMGEEVTLQVFGGTEGIPTNAEVVFLGKAPTLKVSEQLAGRFFNAFGAPI"
BASE COUNT          554 a          349 c          423 g          478 t
ORIGIN      
        1 gggcatcttg tcgagaaatt acgatggtct tcagaatgat ttggacaaaa tggacggagt
       61 ttttcttaaa cgtggcgatt atacgtatgc gcttaacgac gataaactgt gggactttaa
      121 gccattggct aaacccggcg aaaaggtcat tgctggttca tggcttggcg aagtcgacga
      181 aaacagcaag tcacacaaaa taatggttcc ttttgctttc tccggtgaat ataccgtaaa
      241 atcggtaatt gcagcggggc aatataaaat caccgacaca gttgctgttt tgatcaattc
      301 cgacggagaa gaaattgcgg ttactatgat acagcgttgg ccggtaaaac gcccaatcac
      361 cgcttataaa gagaaaccac gtccgtttaa actgctcgaa acgggggtac gtaccatcga
      421 tacaattaac ccaattgttg aaggcgggac tgggttcatt ccagggcctt tcggtaccgg
      481 aaaaacagtg cttcagcacg ccatttcaaa acaggctgaa gctgacatag tggtaattgc
      541 tgcttgtggt gaacgggcca atgaggttgt ggaaatcttt actgagttcc ctgagcttga
      601 agacccacat accggccgaa agttgatgga acggacgatt attattgcaa atacttcaaa
      661 tatgcctgtt gcggcgcgtg aagcctcggt atacactgca atgactttgg ctgaatatta
      721 tcgttcaatg ggactaaaag ttctgatgat ggccgattca acgtcacgtt gggctcaggc
      781 cttgcgcgaa atgtcgaaca ggcttgaaga actccctggg caagatgcat ttccaatgga
      841 cctttctgca attattgcca acttctatgc ccgtgcaggg tttgtatacc ttaacaatgg
      901 ggcaaccgga tcggtaactt ttatcggaac agtttcacca gctggaggaa acctaaaaga
      961 acctgttact gagtcgacaa aaaaagcagc tcgttgtttc tatgcccttc agcaaagccg
     1021 tgccgatagc aagcgttatc cggcaatcaa tcctatcgac tcatattcaa aatatttaga
     1081 ataccctgaa tttgaggaat atattgcaaa aagaattgga gccaactgga ttgaaatggt
     1141 gaacgaagca aaaaccatat tacttcgtgg acaggaggtt agcgagcaaa tcaatatttt
     1201 gggcgacgat ggtgtgcccc tcgattacca cataagattt tggaagtcag aagtaattga
     1261 ttttgttgtc cttcagcagg atgctttcga taaagttgat atgttaaccc cgcttgagcg
     1321 gcaacaatat atgcttaaaa aggtgatgga gatttgccat gccgatttta acttcgatag
     1381 ttttaatgag gttgagaatt actttaaacg tataatcaat cagtataaac aaatgaacta
     1441 ttctgaattc gattcggcgc agttcaaaaa gtacgaagaa gaattagcat caatcattaa
     1501 cgaaaggaaa atagcataat ggcaacaaaa gcatttcaaa aaatatatac caaaattacc
     1561 aaaattacca aggcaacatg cacattgaat gcacaaggta ttggtaacga agagttagct
     1621 gaaatagacg ggcgtttggc acaagttgtt aaaattatgg gtgaagaggt aaccctgcaa
     1681 gtttttgggg gaactgaagg tattccaacc aatgctgaag tggtgtttct tggtaaagct
     1741 ccaaccttga aggtaagtga gcaattggct ggccggttct tcaatgcttt tggggctcct
     1801 attg
//
LOCUS       DAOPUW010000447         1797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_72306_length_1797_cov_2.707807, whole genome shotgun sequence.
ACCESSION   DAOPUW010000447 DAOPUW010000000
VERSION     DAOPUW010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72306_length_1797_cov_2.707807"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PKJ43_07530"
     CDS             <1..754
                     /locus_tag="PKJ43_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72455.1"
                     /translation="FHTIVNANSYFEQESWLLANKTYRPQLARQYAAGIYGTIPGTII
                     EFSAEAYYKAMENLLYYAPPNYENDNLLAFVQKGGQGIAYGGELLLQKTTGTIQWSVA
                     YTLAWSKRKLNNLNSGQWFFADFDRRHDFNLGFHYFKNIKNTWNCNYIFQTGRAYTLP
                     EAYVNATGFYQGYYVSGGVNNARTPNYSRFDVSYKRKLFKRRKVELTLSVMNLFAHKN
                     PFAVYPKDGKMYMSSWYLMMPSAQLKIYLLKD"
     gene            758..1720
                     /locus_tag="PKJ43_07535"
     CDS             758..1720
                     /locus_tag="PKJ43_07535"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HNZ72456.1"
                     /translation="MKVKTLLITFLFALLVYACVDIESEISMNAAYPGDRLAVIAYLS
                     NKGVVVNVQKTVPVENSPFQESKVQNAQVYLYSDDDPDLKIKLYQADDFNFFSEQDFQ
                     PQPGNSYYIVVAAEGFEEVRSSSQVVMNESVIESVTVDISTSYYWTADTTCFFLFGWP
                     RSIYITMNVENSNFLIANSKLQYFVWSGNKRYELNEGKMRQCFYPQYYCRLGMGDFSF
                     DSPVNPISSFLWIDDQLETGDSIFYESKKYPVLQRADSITLQFITASSDFIEYFQKTN
                     YYMENRISPFSSLSEKIPSNLSNGIGYFGNISITECVVILPTFN"
BASE COUNT          537 a          329 c          371 g          560 t
ORIGIN      
        1 gtttcacact attgtgaatg caaacagtta ctttgaacaa gagtcgtggc ttttggccaa
       61 taaaacatac cgtccccagc ttgcgcgaca gtatgctgcc ggtatttatg gaacaatccc
      121 cggtacaata atcgagttta gtgccgaagc gtactacaag gcaatggaga accttttgta
      181 ttatgctccc ccaaattatg aaaacgacaa tttgttagcc tttgtacaaa aaggtggaca
      241 aggtattgct tatggcggag agttgttgct gcaaaaaact accggtacca ttcaatggtc
      301 ggttgcctat acgctcgctt ggtcgaaacg taagttaaac aacctgaaca gcgggcaatg
      361 gttttttgcc gatttcgatc gaaggcacga cttcaatttg ggttttcatt attttaagaa
      421 tattaaaaat acatggaatt gtaattacat ttttcagaca ggacgtgctt acacactgcc
      481 tgaagcttat gtaaatgcaa ccggttttta tcagggatat tacgtctcag gaggcgtgaa
      541 taatgcacgc acaccaaatt acagccgctt tgatgtttcg tataaacgga agttatttaa
      601 aagaaggaaa gtagagctta ccctctcggt gatgaacctc tttgcccata aaaacccttt
      661 tgctgtttat ccaaaagacg ggaaaatgta tatgtcatca tggtatctga tgatgccatc
      721 agctcagttg aaaatctatt tgttaaagga ttaggcaatg aaagtaaaaa cgttgttaat
      781 aacatttctt ttcgcgttgt tggtttatgc ctgtgttgat attgaaagtg aaatatcgat
      841 gaatgctgct taccccggag atcgcttggc tgttattgct tacctaagca ataaaggggt
      901 tgttgtaaat gtgcagaaaa ctgtacccgt tgaaaatagc cctttccagg agtcaaaggt
      961 acaaaatgct caagtttact tatatagcga tgatgatccg gatttaaaaa ttaagcttta
     1021 ccaggccgat gatttcaatt ttttctcgga acaagatttt caaccacagc ctgggaactc
     1081 atattacatt gtagttgctg ctgaaggttt tgaggaagtt agatcgtcgt cccaggtagt
     1141 tatgaatgaa tctgttattg aaagcgttac ggtagatatc tcaacatcgt attactggac
     1201 cgccgatact acctgttttt tcttattcgg atggccccgg agcatataca taacaatgaa
     1261 tgttgaaaac agtaactttc tgattgcgaa tagcaagttg caatattttg tttggtcggg
     1321 aaataaacgg tacgaattga acgaggggaa aatgaggcaa tgcttttatc ctcagtatta
     1381 ttgcaggctg ggtatgggcg atttctcatt cgactcacct gttaacccaa tttcttcctt
     1441 tttatggatc gatgatcaac ttgaaacagg ggatagtatc ttttatgaaa gcaaaaaata
     1501 tccggtgctt cagcgggctg acagtattac attgcagttt attactgctt catccgattt
     1561 tatcgaatat tttcaaaaaa caaattatta catggagaat aggataagcc cattttcatc
     1621 gttatctgaa aaaattccaa gtaatttatc aaatggaata ggctatttcg ggaacatatc
     1681 tattacagag tgtgttgtta tacttccgac tttcaattga aattaatttc acctgactgc
     1741 aagtaatagc aagccttgcc ttgctcatca ctataggctt gctgtttgcc caaaacg
//
LOCUS       DAOPUW010000448         1788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_72874_length_1788_cov_2.655511, whole genome shotgun sequence.
ACCESSION   DAOPUW010000448 DAOPUW010000000
VERSION     DAOPUW010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72874_length_1788_cov_2.655511"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..676)
                     /locus_tag="PKJ43_07540"
     CDS             complement(215..676)
                     /locus_tag="PKJ43_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HNZ72457.1"
                     /translation="MASGIIFGNKTLDVVFISMFAAQLYKFVTSSVIKRKFDWGRLWE
                     TGGMPSSNSSSVVALVTAIAITCGVQSAYFAIAVVFAIVVMYDAAGIRNAAGQHAGVI
                     NQLTEVFSNKYHIKFNNEKLKELLGHSRLEVFVGALVGLAIALLMRNYLLS"
     gene            836..>1788
                     /locus_tag="PKJ43_07545"
     CDS             836..>1788
                     /locus_tag="PKJ43_07545"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNZ72458.1"
                     /translation="MSKSIVFPAEWYPQMAVQLTWPHPDSDWGYLIDEVEKCFVDIAE
                     AIALHQPLIIACKNIDHVKSQVNSKLWSNISFFAIDSNDTWARDHGGITVFDNGKPMI
                     YDFCFNGWGMKFAACHDNQITRKLFKEGAFPDYKYSSMNFFVFEGGSIESDGQGTLLT
                     TEECLLSANRNDSLSKQDIESCLKQFFGAERVLWLKNGYLAGDDTDSHIDTLARFCSA
                     DTIAYVKCTDTTDEHFDALTKMEAELQSFSLSNGEPYRLIPLPMATPVFDPEDGHRLP
                     ATYANFLIINGAVLFPIYHCETDNEAIEAIKTAFPDLEIIP"
BASE COUNT          566 a          415 c          338 g          469 t
ORIGIN      
        1 ctgaaacctg ccgcacgatt aggggtatat ctggcttatc ggtactatct caacctattt
       61 ttataaaatt aagaatgcac gccctgaaga aatactcaag caacgctttc gtatatcgaa
      121 tcgccgcaaa ggggtactaa tgtgtaaggc attaatccgc aatgctataa atgtaattta
      181 aaatgaatta caaaaattta tcaaaaagaa aaaatcagga aagaagataa ttacgcatca
      241 aaagcgcaat agcaagtccg accaatgctc caacaaaaac ttcgagccgg ctatgtccga
      301 ggagttcttt tagtttttca ttattgaatt tgatatgata cttgttcgaa aaaacttcag
      361 tcaactggtt tataactccg gcatgttgac cagctgcgtt tctaatgccg gcagcatcgt
      421 acataacaac aatagcaaaa accacagcaa tagcaaaata cgcactttga acgccacaag
      481 taatagcaat agctgtaacc agtgcaacca ccgatgacga gttggaactt ggcatacccc
      541 cggtttccca aagcctgccc caatcaaact ttcgcttaat gacacttgat gttacaaact
      601 tgtataactg agcagcaaac attgagatga atactacatc aagcgtttta tttccaaaaa
      661 ttatccctga agccatacca caagtttagc attttttctt tctgaaaatt gatttagctt
      721 atccaaaatt aaagaaaaca attaaatatg taaacaattg acagaaaagc ttgtcacaag
      781 ttgcatgaaa aatgattacg agaaattcct atcttcgttt tttattttaa aacacatgag
      841 taaatcaatt gtttttcctg ccgaatggta cccacaaatg gccgtgcagc taacatggcc
      901 tcaccccgat tcagactggg gctatctgat cgatgaagta gaaaaatgct ttgtcgatat
      961 tgccgaggcg atcgcgctac accaaccact gatcattgct tgtaaaaata tcgaccacgt
     1021 aaaatcacag gttaacagca aattatggag caacatctcc tttttcgcta ttgactcaaa
     1081 cgatacctgg gcacgtgacc atggaggcat tacagttttc gacaacggga aacctatgat
     1141 ttacgacttc tgctttaatg gatggggcat gaaattcgcc gcctgccacg acaatcaaat
     1201 cacccgcaaa ctttttaaag agggggcttt ccctgattac aagtattcga gcatgaactt
     1261 ttttgtgttc gaaggtggca gcatcgaatc cgatggacag ggtaccctgc tcaccaccga
     1321 agaatgcctg ctttcggcaa accggaacga ttcgctttcg aaacaggata ttgaaagttg
     1381 cctgaaacaa ttcttcggag ccgaacgggt tttatggctg aaaaacggct acctggccgg
     1441 cgatgatacc gacagtcata tcgataccct tgcccgcttc tgctcggctg ataccattgc
     1501 ttacgtaaaa tgcacggata caacagatga acatttcgat gctttaacaa agatggaagc
     1561 cgagctacaa tcattctcgc tatccaatgg agaaccgtac cggttgatac cgttgcccat
     1621 ggccaccccg gttttcgatc ccgaagatgg acaccgattg ccagccacct atgccaattt
     1681 cctgatcatt aacggagctg tattgttccc aatttaccat tgcgaaaccg acaacgaagc
     1741 cattgaagcc attaaaactg ctttccccga cctggaaatt attcccat
//
LOCUS       DAOPUW010000449         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_73119_length_1784_cov_3.113939, whole genome shotgun sequence.
ACCESSION   DAOPUW010000449 DAOPUW010000000
VERSION     DAOPUW010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73119_length_1784_cov_3.113939"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PKJ43_07550"
     CDS             complement(<1..614)
                     /locus_tag="PKJ43_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNZ72459.1"
                     /translation="MNNENKGLSLALVTVVCWGVLAIGLKVASRAVDSFTIVWIRFAL
                     AFSFLSIWFLLTDRKQFRILIKPPWQLILATIGLSINYIGFQIGVELTSPSIAQVVIQ
                     IGPVLLALAGIFFFHEKIRKQQLLGFGVVIVGFVLFYYEQLKAVFTSPEKFNAGIALL
                     VLGAIAWSVYAVMQKKLVTKYPPQTLNLFLYGLPTILYLPLANF"
     gene            complement(618..1517)
                     /locus_tag="PKJ43_07555"
     CDS             complement(618..1517)
                     /locus_tag="PKJ43_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNZ72460.1"
                     /translation="MWGLIGIISAVLLGIYDIFKKTSLNDNAVLPVLLISIATSNLFF
                     IPIIVGSQVTPEFFVNLGFFCPTLTLAEHIQMVLKASLVLSSWILAFFAIKHLPLTIV
                     SPIRATAPLWTLLGALIIFSENLNLLQWVGLLITLTFFYLFSTTGKLEGIDFKKNKWV
                     FAIIGATLLGSASSLYDKYMIREIDRIAVQAWSGIYQLLLMIIIVGLLWYPKRKNSTP
                     FKWRWTIPCIGIFLTLSDFFYFFALSYPDSLISILSGIRRSGVVIPFIFAALFFKESN
                     IRKKALYLAGIITGVIFMTIGSH"
BASE COUNT          573 a          361 c          363 g          487 t
ORIGIN      
        1 tcaaaattgg ctaagggcag gtataaaatg gttgggagtc cgtataaaaa aaggttaagc
       61 gtttgcggcg gatatttcgt aaccagtttt ttttgcatta ccgcatatac cgaccaagca
      121 atggctccca acacgagcaa ggcaattccg gcattaaatt tctcaggcga tgtaaaaaca
      181 gctttaagtt gttcatagta aaaaagaacg aagccaacga ttactactcc aaaacccagc
      241 aattgttgct ttcggatttt ctcgtggaaa aagaagatcc cagccagggc aagcaacaca
      301 ggtccaattt gaattaccac ctgagcaatg cttggactgg tgagttcaac tccaatctgg
      361 aatccaatgt agttgattga caagccaata gttgccagaa ttagttgcca tggaggcttt
      421 atcagaattc gaaactgttt tctatcggta agaagaaacc aaatggatag aaaagaaaag
      481 gccagggcaa atcttatcca aacgattgta aatgaatcaa cagccctcga agctactttt
      541 aagccaatgg ccagtactcc ccaacaaact acggtaacca gcgccagtga taatcctttg
      601 ttttcgttgt tcatagctta gtgacttcca atggtcataa atattacacc ggttataatt
      661 cctgccaagt ataatgcttt tttcctgata ttgctttctt taaaaaacaa tgcagcaaaa
      721 atgaacggga ttactacacc gctgcgcctt attcccgaca aaattgaaat aagcgagtcg
      781 gggtaactta acgcaaagaa gtagaagaaa tcacttaatg tgaggaaaat accaatgcaa
      841 ggtatagtcc acctccattt gaaaggagtg gagtttttgc gttttggata ccataaaagc
      901 cctacaatta taatcatgag caacaactgg tatattccag accatgcctg aactgcaatt
      961 ctgtcgattt cccgtatcat gtatttgtcg tacaggctac tggccgaccc aagcagggtg
     1021 gcgccaatga tagcgaagac ccatttattc tttttaaagt cgatgccttc gagcttgcct
     1081 gtggtcgaaa aaagataaaa gaaggttaag gtgatcagca aaccaaccca ttggagcagg
     1141 ttcaggtttt cggaaaaaat aataagggct ccgagtaaag tccacagtgg tgctgttgcc
     1201 cgaatcggag aaactatagt caatggcaaa tgcttaattg caaaaaaagc taatatccaa
     1261 ctcgagagaa ccaacgaagc tttaagtacc atttggatgt gctcggccaa cgtgagcgtt
     1321 gggcaaaaaa agcctaaatt cacgaaaaat tcaggagtaa cctgtgatcc aactattatt
     1381 gggataaaaa aaagattgct tgttgctatt gagattaaca ggacggggag tacagcatta
     1441 tcattgagcg atgttttctt aaatatgtcg tagataccca gaagtaccgc gctgataatc
     1501 cctattaagc cccacatttt ttataatatt taattgcaaa tataaaggct gaaactggca
     1561 atcataattt tgcctgtact gttggtgggt tctttaacgg taggttaaag tttatgcatc
     1621 tttttcaacg taaagctgtc ctgattcttg tcgaactact ataatctttt cccccttgtc
     1681 aatgtacgaa tcgttagctt tggcatccca tatatcccca tcgatagaaa cttttccgga
     1741 aggccgtaaa atagtatggg caaaaccttt tttcccaacc aggt
//
LOCUS       DAOPUW010000450         1781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_73320_length_1781_cov_2.797798, whole genome shotgun sequence.
ACCESSION   DAOPUW010000450 DAOPUW010000000
VERSION     DAOPUW010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73320_length_1781_cov_2.797798"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1413)
                     /locus_tag="PKJ43_07560"
     CDS             complement(97..1413)
                     /locus_tag="PKJ43_07560"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNZ72461.1"
                     /translation="MKVYKFGGTSVGSPENMRSVMEIITADGEPKIVVLSAMSGTTNS
                     LVEISNKLYAGDKQAAKALIDTLKQKYDKVVDELYTTLEFKSKGQEVISEHFSTLYDQ
                     VEGTFTEVKEQIILAQGELISTALVTYLLQENGIKTALLPSLDFMRIDEDKQADLPKI
                     KELIKPVLDKAGPQQIYIAQGFICRNAYGDIDNLQRGGSDYTASLLGAAIDSEEVQIW
                     TDIDGMHNNDPRIVDKTTRVDNLLFVEAAELAYFGAKILHPLTVLPAKDANIPVRLKN
                     TMSPSDPGTLISNEKIDSGLKAVAAKDGITAIKIRSYRMLMAYGFLRKVFEIFERYKT
                     SIDMITTSEVSISLTIDNVKNLDHIVDELNTFSEVEIDNNMTIVCVVGDMKSETNFSK
                     QIFDALEGIPVRMISYGGSLHNVSILINTDNKKQTLQKLSDHLFYN"
     gene            complement(1579..>1781)
                     /locus_tag="PKJ43_07565"
     CDS             complement(1579..>1781)
                     /locus_tag="PKJ43_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNZ72462.1"
                     /translation="EKGNEMKSEFFIPMYVDELIKAEKTEVKVLTSNASWFGVTYQED
                     KPVVKDRINQLIAEGKYPSKLW"
BASE COUNT          516 a          365 c          325 g          575 t
ORIGIN      
        1 attctgccag tcatttttaa aatttttcaa taaaaaacag ggcaaccatg atagtacacg
       61 attgccctgc caagtattct atatgaaccg aatcgactaa ttataaaaca aatgatcact
      121 aagcttctgc aaggtttgct tcttattgtc ggtattaata aggattgaaa cgttgtgcag
      181 acttccgcca taagaaatca ttcgaacagg aattccttca agtgcatcga aaatttgttt
      241 tgagaaattg gtttccgact tcatatcgcc cacaacacaa acaatggtca tgttgttatc
      301 tatttctact tcgctgaatg tattcaattc gtctacaata tgatcgagat ttttaacatt
      361 atcgatagtt aacgaaattg agacttcaga tgtggtaatc atatcgatag aagttttgta
      421 acgctcgaaa atttcgaata ccttccgaag aaaaccataa gccattagca ttcggtacga
      481 gcgaattttt atggctgtga tcccatcttt ggcggccacg gctttcaatc ccgagtcaat
      541 tttttcgttc gaaataagtg ttcccgggtc tgaagggctc atcgtgtttt taaggcgcac
      601 aggaatattg gcatccttgg ctggcaaaac agtaagcggg tgaaggattt tggcaccaaa
      661 ataagccaac tctgcagctt caacaaaaag aaggttatct acacgggtag ttttatcaac
      721 gatgcgtgga tcgttgttgt gcattccgtc aatatcggtc caaatctgca cctcttctga
      781 gtcgattgct gcaccaagaa gtgaggcggt ataatcgctg ccaccccgtt gcaggttgtc
      841 gatgtcgcca taggcattcc tgcaaataaa cccttgagca atatatattt gctgaggccc
      901 agctttgtca agtacaggtt tgataagttc tttaatttta ggcagatcgg cctgtttatc
      961 ttcatcgatt cgcataaaat cgagtgaagg aagcaacgct gtttttatac cgttctcctg
     1021 caaaagataa gtcaccaaag cggtagaaat tagttcaccc tgagcaagga taatttgttc
     1081 cttaacctcg gtaaatgttc cctcaacctg atcatacaaa gttgaaaaat gctcactgat
     1141 aacttcttgg cctttgcttt tgaattctaa agtagtatat aattcatcaa caactttatc
     1201 gtatttctgt ttcaatgtat cgataagagc cttggcagct tgtttatcgc cggcataaag
     1261 tttattactg atttcgacaa gcgagtttgt tgttcccgac atcgctgaaa ggacaacaat
     1321 cttcggttcg ccatcggcgg taataatttc cattaccgac cgcatattct caggagaccc
     1381 caccgaggtt ccgccaaact tgtaaacttt catgtatatt caatttaatt attgtaagat
     1441 tgcgcaaata tgtgaaaaat tagttttcca gtatatacgt gaaaccctga atcaacaatt
     1501 gttagccttc atttcataca gagttaaaaa gcttttgtac tatatttatt tctaaagctt
     1561 tctttctgaa aacattcatc accaaagttt tgatggatat tttccttcag caatcaactg
     1621 gttaatacga tccttcacaa ctggtttatc ttcctgatag gtgaccccga accatgaagc
     1681 attgcttgta agtactttaa cttctgtttt ttctgctttg atcaactcgt caacatacat
     1741 aggaataaag aattcagatt tcatttcgtt gcctttttct t
//
LOCUS       DAOPUW010000451         1778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_73503_length_1778_cov_3.417876, whole genome shotgun sequence.
ACCESSION   DAOPUW010000451 DAOPUW010000000
VERSION     DAOPUW010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73503_length_1778_cov_3.417876"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PKJ43_07570"
     CDS             <1..209
                     /locus_tag="PKJ43_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72463.1"
                     /translation="NNVRLPAVHHLDISFFLENTAKEKSSWVFSIYNVYNKKNTSFYF
                     IENNKIKGAALLPIIPSITWNYKF"
     gene            complement(294..812)
                     /locus_tag="PKJ43_07575"
     CDS             complement(294..812)
                     /locus_tag="PKJ43_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72464.1"
                     /translation="MRRIVDKANCTEIGYVKKTHGVKGEVQIVFHDGLDEMIERLDYL
                     FFEVEGLLVPFFIDSIFSIGPNSAILLFETLGNKEEATAFVGCKLFIDTVELDGSEVE
                     FSPQLLKGFTLIDQHRKERGSITAVDDYGGNLVFTVGKGRNELLVPYHDDLLIEFDPR
                     RKKIVMHCPEGL"
     gene            complement(1347..>1778)
                     /locus_tag="PKJ43_07580"
     CDS             complement(1347..>1778)
                     /locus_tag="PKJ43_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72465.1"
                     /translation="WQNSTRRFASINNGDVFPFDYDRTFELNCYGQYAFSDNVSLGVT
                     WTYATGIPANIPEWRYSDINDDFVFLYNGYNSSRQKDYHRLDIGLTLKGDRGDWNISV
                     INAYARKNTYYHEVLAYEETPKLKDRSLFTILPTLSYSFKF"
BASE COUNT          527 a          394 c          342 g          515 t
ORIGIN      
        1 caaacaatgt aagactcccg gcagtgcatc acctcgacat ttcgtttttt ctcgagaata
       61 ctgcgaaaga aaaaagttcc tgggtattta gcatttacaa tgtctacaac aagaaaaaca
      121 caagcttcta tttcattgaa aacaacaaga taaaaggagc agcacttttg ccaataatcc
      181 catcaataac atggaattac aaattttaat ggatgtgctt gcatccttat tgtgaattat
      241 cagctaatta gttaaaataa gtggcaaaat cccaaactct ctccaatggt tcgctacaag
      301 ccttcggggc agtgcataac aatcttcttc cggcggggat cgaattctat cagcaggtca
      361 tcgtgataag gcacaagcag ctcgttacgg cctttgccca cggtaaaaac caggttgccc
      421 ccgtaatcgt caaccgcggt gatgcttccc cgttctttcc ggtgctggtc aatcaaggta
      481 aagcccttta aaagttgtgg tgaaaactca acttcggaac catcaagctc cacggtatca
      541 ataaacaact tacaaccgac aaaggctgtt gcttcttctt tgttgccaag tgtttcgaaa
      601 agcagtatcg ccgaattggg gcctatggag aagatagaat caataaaaaa aggcaccagc
      661 aacccttcca cttcaaaaaa gagataatcg agcctttcaa tcatttcatc aagcccatcg
      721 tgaaagacaa tttgcacttc gcccttcacg ccgtgggttt tctttacata ccctatttcg
      781 gtacagtttg ctttatcaac aattcttcgc atactacatg tttatgacgc aaaaatagcg
      841 aatttgaaac gatctgtttg tgcctgattg ttttaaaatg aatatccccg gctgtaccta
      901 aaactgattc atgaaactta ttttttaatt gccacgggcc aaaaaattag aattgggcta
      961 aagcccagca taatatgagc tttgattttg tccacctgaa ggtagtggca attaaaaatc
     1021 atcatcaaca attatttaga actcactttt gatgtttagg aatgatatcg gctattcata
     1081 ttgttttaaa attcagacca gtttttttaa ctcaaaagat aatagttctg ctctaaaaga
     1141 ttataaaccc ccgctgccgc gcgattgtat cgcgtgactt tcagcagtct attctacttc
     1201 gtcactatca catcgtccag caatcctttt ctgacagcgt agtctactgc actgctgtaa
     1261 atgtaatctt ctgacctaaa aataaggaat atatttaatt gaccacgcga taaaatcgcg
     1321 cggcagcaaa gtcggtcagc ctattttcag aatttaaacg aataacttaa tgtgggtaaa
     1381 atggtaaaca agcttcggtc tttgagcttt ggtgtttctt cataagccag cacttcgtga
     1441 tagtacgtgt tctttcgggc atacgcattt attacgctga tgttccagtc gccacgatcg
     1501 cctttcaggg taagccctat atccagccga tggtaatctt tttggcgcga actgttatat
     1561 ccgttgtata gaaaaacgaa gtcatcgtta atgtctgagt aacgccactc gggaatgttg
     1621 gccggaattc cggtggcata cgtccaggta acgccaaggc ttacattatc agagaaggca
     1681 tattgcccat agcagttcag ctcgaatgta cggtcgtaat cgaacgggaa aacatctccg
     1741 ttgttgatac tggcaaacct gcgggtactg ttttgcca
//
LOCUS       DAOPUW010000452         1770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_74075_length_1770_cov_2.262974, whole genome shotgun sequence.
ACCESSION   DAOPUW010000452 DAOPUW010000000
VERSION     DAOPUW010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74075_length_1770_cov_2.262974"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PKJ43_07585"
     CDS             <1..562
                     /locus_tag="PKJ43_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72466.1"
                     /translation="GRIDRLGQKSNKLTIFNLITRDSIEQQIAAGLLVKQSLFDGVLG
                     TNANTNFVDFSSKGRSQFIDQLEEFIASQDLMPDATYRDDEFRDELEPVAEKTSIAEE
                     IDLSEDRSELPGETIIAEGKAPEQKPQAPQPEEIEQVMNNGLQFLAGLFKMSTGKDIG
                     MEGQKIEINRETGEVTMKFKLPGFGQ"
     gene            599..1417
                     /locus_tag="PKJ43_07590"
     CDS             599..1417
                     /locus_tag="PKJ43_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNZ72467.1"
                     /translation="MISYMNVTQKKLEVINPGLKDYINDYYVFEFESELPNNFTFKVP
                     PTGFPTMLFCFGNKMNFFQHKHLTNESIIVGQLSKHINLHPVTGTKIFGVNFKPYGFY
                     NLFNKSLKDLKNSAIESSVIFDGESITFIEELLTKNCPINDVMYSIESLLLFHKKEAV
                     KNDFFDGIVDEIVKMNGLADPFQMATKNASIRSFQRYFNEVIGINPKVFCQVLRHKFI
                     LQLLYMNPDLKWNELILGGYYYDYSHFQKDFLKFTDVKPIDYLPYINPFAQKLL"
     gene            1507..>1770
                     /locus_tag="PKJ43_07595"
     CDS             1507..>1770
                     /locus_tag="PKJ43_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72468.1"
                     /translation="MKIKLLTAKRLMLGVCLTTLINNTFSQNSDQISWNKYDFVPGTE
                     IIFEDNLGGEQNGEFPSKWDLVGGNVENANLNAENVLMFIEMGA"
BASE COUNT          593 a          323 c          341 g          513 t
ORIGIN      
        1 tggccgaatc gaccgccttg ggcagaaaag caataagctc accattttca atcttattac
       61 ccgcgacagc attgagcagc aaattgccgc cgggcttttg gtaaaacaaa gcctgttcga
      121 tggcgtactt ggtacaaatg ccaacaccaa ctttgtcgat ttttcgtcga aagggcgttc
      181 acagtttatc gaccagctcg aagagttcat cgcttcgcag gatctaatgc cagatgccac
      241 ttacagggat gatgaattcc gagacgaact tgaacctgtt gctgaaaaaa cgagcattgc
      301 cgaagagatc gacctttcgg aagaccgttc cgaattgccc ggtgaaacaa ttattgcaga
      361 aggaaaagca cctgaacaaa agccacaagc accacagccc gaagaaattg agcaggtaat
      421 gaacaatggt ttgcagtttc ttgccggctt gttcaaaatg agcacgggca aagacattgg
      481 catggaagga caaaagattg aaataaaccg cgaaaccggc gaagtaacta tgaagtttaa
      541 actgccaggg tttggccaat agttatattt aaacgaatag ccagataaag accactattt
      601 gattagttac atgaatgtta cccaaaagaa gttagaagta atcaaccccg gattaaaaga
      661 ctatattaac gactattatg tttttgaatt tgaaagtgag ctgcccaata actttacatt
      721 taaagttccg cctacaggtt tccctaccat gttgttctgt tttgggaaca aaatgaattt
      781 ttttcagcat aaacatttaa ccaatgaatc aataattgtt gggcagctct cgaaacacat
      841 taacctgcat cctgttaccg gcacaaagat atttggtgtt aatttcaaac cttatggttt
      901 ttataacctc ttcaacaaat cactgaaaga ccttaaaaac agtgctatag aaagttccgt
      961 tattttcgat ggtgaatcta ttaccttcat tgaggaactt ctaacaaaaa attgcccaat
     1021 taacgatgta atgtacagta ttgaaagctt gttacttttt cataagaaag aagctgtgaa
     1081 aaatgatttc ttcgatggaa ttgttgacga aattgtgaaa atgaacggat tagcagatcc
     1141 attccaaatg gcaacaaaaa atgcctcaat aagaagtttc caacgttatt ttaacgaagt
     1201 gataggaatt aaccctaagg tattttgtca ggttttgagg cataaattta tacttcaact
     1261 tttatacatg aaccctgatt tgaaatggaa cgagctgatt ttgggtggtt actattatga
     1321 ctactcacac tttcaaaaag attttttaaa gttcaccgat gttaaaccaa ttgattattt
     1381 gccttatatt aatccatttg ctcaaaagtt attgtagttg aacatgtttt aacaaacact
     1441 gtttgtcgct tttttacagt aaacaattat ctaattcgtt ttcttttgca gaaagaaaaa
     1501 gaacagatga aaataaaatt acttactgca aaaagattaa tgcttggggt gtgtcttacc
     1561 accttaataa acaatacatt ttctcaaaat agcgatcaaa taagttggaa taagtacgac
     1621 tttgttccag gtactgaaat tattttcgag gataacctgg gtggggaaca aaacggtgaa
     1681 ttcccaagca agtgggactt agttggcgga aatgttgaaa acgccaacct taatgccgag
     1741 aacgtactga tgtttatcga aatgggtgct
//
LOCUS       DAOPUW010000453         1766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_74344_length_1766_cov_2.536528, whole genome shotgun sequence.
ACCESSION   DAOPUW010000453 DAOPUW010000000
VERSION     DAOPUW010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74344_length_1766_cov_2.536528"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PKJ43_07600"
     CDS             <1..195
                     /locus_tag="PKJ43_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HNZ72469.1"
                     /translation="AVCHFMGETTETRHVWMKNEQAILSPAWSIHSAAGTANYIFIWG
                     MAGENLDYGDMDVCQPDELR"
     gene            219..560
                     /locus_tag="PKJ43_07605"
     CDS             219..560
                     /locus_tag="PKJ43_07605"
                     /inference="COORDINATES: protein motif:HMM:NF017830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNZ72470.1"
                     /translation="MKYVCKYNEQLPTKVTDWYDRRTISTSKLMLVTAEFHDGPCEKP
                     DPFHQHPHEQVSYMADGEVYLLVDNEPAVHLTAGDTFAIPSNVPHTIQRLTPYVRIID
                     CFTPIREDFLK"
     gene            592..1395
                     /locus_tag="PKJ43_07610"
     CDS             592..1395
                     /locus_tag="PKJ43_07610"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HNZ72471.1"
                     /translation="MIQELFDLTGKVALITGGTHGIGMAIATTLGKAGATIVVNDLSE
                     EKLMAAKAEYKKNGIDTYTLIFDVTDEKAVDRGISEIEKNVGPIDILVNNAGIIKRIP
                     ILDMPVSDFKQVIDIDLVAPLIVSKRVVPNMIKKRSGKIINMCSMMSVYGRNTVSAYA
                     SAKGGLKLLTANMTCEWAKYNIQINGIGPGYIVTSQTGPIRENGHPFNDLVMTRTPAG
                     RWGQPEDVGHAALFLASKASDFVNGHILYVDGGILANFGYVKGENDLPE"
     gene            complement(1398..>1766)
                     /locus_tag="PKJ43_07615"
     CDS             complement(1398..>1766)
                     /locus_tag="PKJ43_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72472.1"
                     /translation="ELFMLWAVNKGSVGGYFANLFFFTVVSYGAAHVIFFLGRFLSSR
                     FSAKLSKLQFYQKMLPTVKKYGAPLIIIAALTPVPWTTVALIMGAIGFDYKKFTLYGL
                     SRVARFIVYGYVVFHTHHLF"
BASE COUNT          581 a          375 c          375 g          435 t
ORIGIN      
        1 gctgtttgcc acttcatggg tgaaacaacc gagacccgcc acgtatggat gaaaaacgag
       61 caagccattc tttctccggc atggagcatc catagcgccg ctggtactgc aaactatatt
      121 tttatttggg gaatggcagg cgaaaatctc gactacggcg acatggatgt atgccaacct
      181 gacgaattgc gttaacgtct gaataaataa tgaatcagat gaaatacgtg tgtaaatata
      241 acgaacagct accaaccaaa gtaaccgatt ggtacgacag aagaaccata agcacaagta
      301 agctaatgct tgttactgct gaatttcatg acgggccttg cgaaaaacct gacccgttcc
      361 accagcaccc tcacgagcaa gtttcgtata tggccgatgg cgaagtatac cttttggtag
      421 ataacgaacc tgcggttcat cttactgctg gcgatacgtt tgccatacca tcaaatgtgc
      481 cacacaccat tcaacgactc acaccctatg tgcgtattat tgattgtttt actcccatta
      541 gggaagattt tttgaaataa caaattcatt gaacattaaa caaatacaaa aatgatacaa
      601 gaattattcg atttaacagg aaaagtagct ttaataaccg gaggaacaca cggtattggg
      661 atggctattg ccaccacatt gggcaaagct ggagctacaa ttgttgtaaa tgacctttcg
      721 gaagaaaaac ttatggcagc caaagccgaa tataaaaaaa acgggatcga cacatacact
      781 cttattttcg atgtaaccga tgaaaaagcg gtggaccggg gtattagtga aatagaaaaa
      841 aatgttggac caattgatat tttggtgaat aatgccggga tcatcaagcg gattccaatc
      901 ctcgatatgc ccgtctccga cttcaaacaa gttatcgaca ttgaccttgt tgccccacta
      961 attgtttcaa aaagagttgt accaaacatg attaagaaac gcagtggcaa aattatcaac
     1021 atgtgctcaa tgatgagcgt atatggacga aacacggtct cagcatacgc ttcggctaaa
     1081 ggaggtttaa agttactcac cgccaacatg acctgcgagt gggcaaaata caacatacaa
     1141 atcaacggga ttggccctgg ctatatcgtg acatcgcaaa ccggtccaat tagagagaac
     1201 ggccatcctt tcaacgactt ggttatgacc cgaactccag ctggccgttg ggggcagccc
     1261 gaggatgttg gccatgcggc actcttcctt gcatcgaaag ccagcgactt tgtcaacggg
     1321 cacattttgt atgtagatgg aggtattttg gcaaactttg gctatgtgaa aggagaaaac
     1381 gacttaccgg aataaaatta aaaaaggtgg tgtgtatgaa aaactacata cccatataca
     1441 ataaaacgcg caacacggct tagcccgtat aatgtaaact ttttataatc aaaacctatg
     1501 gcgcccatta ttaatgcaac cgttgtccat ggaaccggtg ttaatgcagc aataataatt
     1561 agtggggcgc catatttttt tacagtaggt aacatctttt gataaaattg aagtttagat
     1621 aatttcgctg aaaaccggct agatagaaaa cgtccaagga aaaaaatgac atgggcagcg
     1681 ccatacgaca ccactgtgaa gaaaaatagg tttgcaaaat agcctccaac agaacctttg
     1741 ttcacagccc aaagcataaa cagttc
//
LOCUS       DAOPUW010000454         1762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_74596_length_1762_cov_3.106034, whole genome shotgun sequence.
ACCESSION   DAOPUW010000454 DAOPUW010000000
VERSION     DAOPUW010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74596_length_1762_cov_3.106034"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /gene="asnB"
                     /locus_tag="PKJ43_07620"
     CDS             complement(<1..1107)
                     /gene="asnB"
                     /locus_tag="PKJ43_07620"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HNZ72473.1"
                     /translation="MCGIVGIFDIKQEPQSLRSQALKMAKRIRHRGPDWSGIYCGDKA
                     ILAHERLSIVDPSSGKQPLYSKDGKLVLAVNGEIYNHQDYRKKYADNYEFLTQSDCEV
                     ILALYRDKGVDFLEDMNGIFAFALYDIEKDEYLIARDHIGIIPLYIGWDSMGTFYVAS
                     ELKALEGYCVKIEEFKPGHYLSSKDGKMTRWYKRDWMEFDSVKNNPASISELKEALEN
                     AVHRQLMSDVPYGVLLSGGLDSSIISAIAKKYAAKRIESGNTQEAWWPQLHSFAVGLV
                     GSPDLAAARKVADHIGTVHHEIHFTVQEGLDAIRDVIYHLETYDVTTVRASTPMYLLA
                     RVIKSMGVKMVLSGEGADEIFGGYPWYGNKAQRDN"
     gene            complement(1122..>1762)
                     /locus_tag="PKJ43_07625"
     CDS             complement(1122..>1762)
                     /locus_tag="PKJ43_07625"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ72474.1"
                     /translation="LHQQNALVSQKEVPGPELISAKGKKVLVIGGGDTGSDCVGTSIR
                     QGATSVIQIELLPQPPENKNPDTPWPMYPNVLKTSSSHKEGCERRWSLNTKQILGKKD
                     AVSGVVVEEVEWSKDENGAWKMTPTGKTETIKADLVLLALGFIHPVHEGLLDELGLSY
                     DNRGNVEVNALMQTSKSKIFAAGDTITGASLVVRAIDSGRKVAEKIDSFLKQ"
BASE COUNT          434 a          415 c          365 g          548 t
ORIGIN      
        1 gttatcgcgc tgggctttgt ttccatacca ggggtagccg ccgaaaattt catcagcacc
       61 ttcgcccgag agtaccattt ttacgcccat cgacttaatt acccttgcaa gtaaatacat
      121 tggcgttgat gctcttacag tggttacatc gtaagtctca aggtggtaaa ttacatcgcg
      181 gatagcatcg agaccttcct gaacggtgaa atgtatctcg tgatgaacag ttccgatatg
      241 gtctgccact ttccgagctg cagcaaggtc gggagatcca accaacccaa cagcaaaaga
      301 atgtaactgt ggccaccatg cttcttgcgt gtttccactt tcaatgcgtt tggctgcata
      361 tttttttgct atggccgaaa tgatcgacga atctaaccct cccgaaagga gtaccccgta
      421 aggaacatcg gacataagtt ggcgatgtac agcattctca agtgcttctt tcagttcaga
      481 aatgcttgcc gggttatttt taaccgagtc gaattccatc cagtcgcgct tgtaccagcg
      541 ggtcatcttc ccatctttgc tcgacaaata atggcctggc ttgaattctt caattttcac
      601 acagtaacct tcaagtgctt ttaattcaga ggcgacataa aatgttccca ttgaatccca
      661 cccaatatac aatgggataa tcccaatatg atctcgagca ataaggtatt catctttttc
      721 tatatcgtac aaggcgaaag caaaaatgcc gttcatatct tccagaaaat caactccttt
      781 atcgcggtat aatgccaata taacctcgca atctgattgc gtaagaaact cataattatc
      841 ggcatatttt tttctgtaat cctgatggtt gtaaatctcg ccgttcacgg ccagaaccag
      901 cttgccgtct ttgctgtaca aaggttgttt tcccgagctg ggatcaacaa tcgacaaacg
      961 ttcgtgggca agaatggctt tgtcgccaca ataaatgccc gaccaatctg gtccgcggtg
     1021 acgaatgcgt tttgccattt ttagtgcctg agatcgtaac gactgaggct cttgttttat
     1081 atcgaatatt ccaacaattc cacacataag taattcgttt tttattgttt taaaaagcta
     1141 tcgatttttt ctgccacttt tctgcccgaa tcgatagccc taactacgag gcttgctccg
     1201 gtaatggtgt cgccggctgc aaatattttc gatttgctgg tttgcatcag tgcatttact
     1261 tcaacattgc ccctattgtc gtagcttagt ccgagttcgt cgagcaggcc ttcgtgaaca
     1321 gggtgtatga aaccaagtgc caataaaacc aaatcggctt ttattgtttc tgttttacct
     1381 gttggagtca ttttccaagc tccgttttca tctttactcc attcaacttc ttcaacaaca
     1441 actccggata cagcatcttt tttgccaagt atttgtttgg tgttgaggct ccagcgcctt
     1501 tcgcaacctt ctttgtgcga gctcgatgtt ttgagtacgt tagggtacat tggccaaggt
     1561 gtgtcggggt ttttattctc aggtggctga ggtaaaagtt caatttggat aacacttgtg
     1621 gcaccttgcc tgattgaagt tccaacgcag tcagaaccgg tgtcgccacc accaatgacc
     1681 aatacttttt taccttttgc tgaaatcagc tcggggcctg gtacttcttt ttgcgaaaca
     1741 agtgcgtttt gttgatgcag ga
//
LOCUS       DAOPUW010000455         1760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_74734_length_1760_cov_3.097947, whole genome shotgun sequence.
ACCESSION   DAOPUW010000455 DAOPUW010000000
VERSION     DAOPUW010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74734_length_1760_cov_3.097947"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..597
                     /locus_tag="PKJ43_07630"
     CDS             202..597
                     /locus_tag="PKJ43_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNZ72475.1"
                     /translation="MKIDHIAIWARDLEKLKYFYVKYFGMTCGEKYMNPTKHYSSYFL
                     TFEGAETCIEIMHFPDIAERTEKQSATMGIAHFSIALDSKEQVDNLTERLRIDGFNIV
                     GEPRTTGDGYYESVVEDCEGNWVEITVST"
     gene            771..1220
                     /locus_tag="PKJ43_07635"
     CDS             771..1220
                     /locus_tag="PKJ43_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HNZ72476.1"
                     /translation="MIVDKIENAHIYFSINSLLGKGLRYILAQNLDEVQPGKTVLEGE
                     SLFAMVNEYDTKPAEQCKPEAHRRYTDIQFMVSGEEKIGFTPYNGQTPMEEFNTENDI
                     MFFNLETDQFTLKPGYFAVFFPHDIHQPGIMVGTPSEVKKVVVKVEL"
     gene            1455..>1760
                     /locus_tag="PKJ43_07640"
     CDS             1455..>1760
                     /locus_tag="PKJ43_07640"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422435.1"
                     /note="catalyzes the formation of fructose
                     1,6-bisphosphate from fructose 6-phosphate and
                     diphosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HNZ72477.1"
                     /translation="MAISALQQERAKYQPKLPKALQGNVKAIEGKATESVADQTEIKK
                     LFPNTYGLPLITFQASDTKPSFPAFNVGVILSGGQAPGGHNVISGIFDGLKALNPDNK
                     "
BASE COUNT          542 a          310 c          367 g          541 t
ORIGIN      
        1 acaattcacc atcgatccat cgaaattggc cttttggaac atcaacatgg aacatgttgt
       61 tgaaccgggc gagtttgaag tgatggttgg cacttcttca atcgatttgt tgacgaaaac
      121 ctttgtggtt gagtaatatt ctcgaagaat atcgcaattg gtttagaagc aaactagtca
      181 aatagtccaa aactagacat catgaaaata gaccatattg ccatttgggc acgcgacctt
      241 gaaaagctca aatatttcta cgtgaagtac tttggtatga cgtgtggcga aaagtatatg
      301 aaccccacaa agcattattc atcgtacttt ttgacttttg aaggagccga aacgtgcatt
      361 gaaattatgc actttcccga cattgcggaa agaacagaaa agcagtcggc cacaatgggc
      421 atagctcatt tttcaattgc cctcgatagc aaagaacagg ttgacaatct taccgaacgt
      481 ttacgaatcg atggtttcaa cattgtaggc gaaccccgca ccaccggcga tggctattac
      541 gaaagtgtgg tagaagactg cgaaggaaac tgggttgaaa ttacggtttc aacgtagaac
      601 cggttaagct ttcacaattt gagcgttctc agattgttga ggttgctcca aaaattgatt
      661 aattcaacat gcgcttctga aactgccggt tgttacttat tattttttgt tattgggatt
      721 ttttgaagga agaatagtat ttttcgggta taatacacaa cacaataatt atgattgtcg
      781 ataaaattga aaatgcccat atttatttca gtattaattc tttgctgggt aaaggcttga
      841 gatatatttt agctcaaaat ttagatgagg ttcagccagg taaaacggtt ctcgaaggag
      901 aatcactttt tgccatggta aatgaatacg atacaaaacc tgcagaacaa tgtaagcctg
      961 aggctcaccg tcgttatacc gatatccagt ttatggtttc gggggaagaa aaaataggat
     1021 ttactcctta taacgggcaa actccgatgg aagaattcaa tactgaaaat gatatcatgt
     1081 tttttaatct cgaaactgac cagtttacgt tgaaacccgg gtattttgct gttttctttc
     1141 ctcacgacat ccatcagcct gggattatgg tcggtacccc atcggaagtg aaaaaagttg
     1201 tggtaaaagt tgagttgtga atgttggcgt tgttatttcc ttaggttgaa ggttgttgat
     1261 attctgttag atgtaatatg ctgacattcc tttttgtgga ttacagattt tgtacaaatc
     1321 tcaacattaa cttattgttt tttaattggt cgtcggcttg ttatttaaaa cctattggta
     1381 ggtaaatcgg tcgatttgat ttatttttag tccgtttttc gaaaaattaa tatcaaaaaa
     1441 taaaattcaa tacaatggca attagtgcac tacaacaaga aagggccaag tatcagccca
     1501 agcttcccaa agcattacag ggaaatgtta aagcgattga aggaaaagct actgaatcgg
     1561 ttgctgacca gactgaaatc aagaaattat tcccaaatac ctatggtttg ccgctgatta
     1621 ccttccaggc aagtgacaca aaaccgtcgt ttcccgcttt caatgttggg gtaattcttt
     1681 ccggaggtca ggctcctggc ggacacaatg taatttcagg aatttttgat ggattaaaag
     1741 cattgaaccc tgataataaa
//
LOCUS       DAOPUW010000456         1759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_74821_length_1759_cov_2.411972, whole genome shotgun sequence.
ACCESSION   DAOPUW010000456 DAOPUW010000000
VERSION     DAOPUW010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74821_length_1759_cov_2.411972"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PKJ43_07645"
     CDS             <1..716
                     /locus_tag="PKJ43_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNZ72478.1"
                     /translation="NEDMEGKLIPGICGQVDGSVIPGMIGLEAGQSAFGDVYAWFKNV
                     LAWPLNFIDDAELRKSIEDKLIPALSDEAAKIPVEESTIIATDWLNGRRTPDADQNVK
                     ATITGLTLGSSAPRIFRALVEATAYGSKAIAERFIENGIEIKQVVGIGGVAKKSSFVM
                     QTLADVMNMPIKVARAEQCVALGAAMFAAVASGLYPTVEEAQKAMGQGFEKTYYPIQH
                     NVEVYEKLYREYQRVGSIL"
     gene            724..>1759
                     /locus_tag="PKJ43_07650"
     CDS             724..>1759
                     /locus_tag="PKJ43_07650"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNZ72479.1"
                     /translation="MRLLTNSIVAILGLAAMVSCSTPETEKAGYPIKAVGITNVEVTD
                     AFWLPKIQEVQTITIDHSLKKCETEGRMENFLVAGGKKEGTYKGKMPFDDTDLYKIIE
                     GASLSLISNPNPVLETYLDSIISIIAVGQEPDGYLTTWFTIDRKTPPAWWVKPSTNRW
                     ENEQSSHEHYNSGHMFEAAAAHYRATGKRNFLDIALKNADLLTENFGPGKLTTPPGHQ
                     IIETGLIQLYEITGNKKYLDLSKFFLDERGDSTHRQLWGPYNQDHLPVTQQTEVVGHA
                     VRAVYQYAGMTDIAVLFRDSSYLNAVNALWENMVNKKMYITGGLGARHDGEAFGDNYE
                     LPNKTAYSETC"
BASE COUNT          576 a          358 c          397 g          428 t
ORIGIN      
        1 cgaacgaaga tatggaagga aaactaatcc cgggcatctg cggacaagtt gatggttctg
       61 taattccggg catgataggc ctcgaagctg gacaatcggc ctttggtgac gtatatgcat
      121 ggtttaaaaa tgtacttgcc tggccgttga attttattga cgatgcagaa ttacgcaaga
      181 gtattgaaga caaactcata ccagccttat cagatgaagc tgctaaaatt ccagtggaag
      241 aatcgaccat tattgcaacc gactggctca acggacgacg cacccctgat gctgaccaaa
      301 atgtaaaggc aacaataact ggcttaacac taggatcatc agctcctaga attttcaggg
      361 cactggttga agctacagct tatggttcaa aagcaattgc tgaacgtttt attgaaaatg
      421 gtattgaaat aaaacaagta gttggtatag gtggagttgc aaaaaaatct tcattcgtaa
      481 tgcaaacctt ggccgatgta atgaacatgc caattaaagt ggcccgtgca gaacaatgtg
      541 tagccttggg tgcagccatg ttcgccgcag tagcttcggg attataccct accgttgaag
      601 aagcacaaaa agcaatggga cagggatttg aaaaaaccta ctatccaatt cagcataatg
      661 ttgaagtata tgaaaaactt tatcgcgaat atcagcgtgt aggttcaata ctataaaatt
      721 attatgaggc tgttaactaa ttcaatagta gcaatattgg gacttgcagc aatggtatcc
      781 tgctcgactc ctgaaacgga gaaggcagga tatcccatta aagctgtcgg cattaccaat
      841 gtagaagtta ccgatgcttt ttggcttcca aaaatacagg aagtacaaac aatcaccatc
      901 gaccactcac tcaaaaagtg tgaaacagag ggccgtatgg aaaactttct ggtagctggg
      961 ggaaaaaaag aaggaactta taaaggtaaa atgcccttcg acgacactga tttgtacaaa
     1021 attatcgaag gggcttcatt atcactcatc agcaatccga atccagtgct cgagacttac
     1081 ctcgactcga taatctcgat tattgctgta ggtcaggaac ctgacggata cctaacaaca
     1141 tggtttacca tcgacaggaa aactcctccg gcctggtggg taaaaccatc aacgaaccgt
     1201 tgggagaacg agcaatcgag tcacgaacac tataacagcg gacacatgtt tgaagctgcc
     1261 gctgcacatt accgtgccac tgggaaaaga aattttctgg atattgcgtt gaaaaatgct
     1321 gatctgttga cagaaaactt tggcccggga aaactcacaa cacctccggg tcatcaaatt
     1381 attgagacag gattgattca gttgtatgaa attaccggga acaaaaaata tctcgatcta
     1441 tcgaaatttt tccttgatga aaggggcgac tcaacccatc ggcaactatg gggtccatac
     1501 aatcaggacc atttaccagt aactcaacaa acagaagtgg ttgggcatgc ggtaagagca
     1561 gtttatcaat atgcgggaat gaccgatatt gctgtgcttt ttcgcgactc gagctacctt
     1621 aatgcggtaa atgcactttg ggaaaacatg gtaaataaaa aaatgtacat caccggagga
     1681 ttaggtgcaa gacacgatgg agaagcattt ggcgataatt acgaactgcc caataaaaca
     1741 gcctacagcg aaacctgtg
//
LOCUS       DAOPUW010000457         1758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_74870_length_1758_cov_2.841456, whole genome shotgun sequence.
ACCESSION   DAOPUW010000457 DAOPUW010000000
VERSION     DAOPUW010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74870_length_1758_cov_2.841456"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PKJ43_07655"
     CDS             <1..360
                     /locus_tag="PKJ43_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNZ72480.1"
                     /translation="NTPYGGFGLWLTPRDLAKVGLMLLNNGQYNDHQILSADYIKQAT
                     TAKTIINEKDYGYLFWVDHSGRYFWTWGAGGQFIFVVPEKELVVVITSEQFADEQNTT
                     FEQASYLVEKIMEGVKN"
     gene            579..>1758
                     /locus_tag="PKJ43_07660"
     CDS             579..>1758
                     /locus_tag="PKJ43_07660"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HNZ72481.1"
                     /translation="MKSILIGIPTSCRTILLGAILLLMGSSTFAISVTFRVDMTHFIT
                     MGFFKPGVDTVLVVGDFNNWAINKQLARVGSTNIYSTSLTVNSNSTYEYKFYCTGTSS
                     YNDTHIDSFEGLVGDGKNGNRILQTGTTDITLPTVFYSNFKPGNAIAKQKAETIAKNW
                     YKHWNPDPKATSEISEYIYEEQFKQPFAHIFNFSSGGWVVVSATDRLRPIMCYNFINR
                     AQYMGANAYNSLISLETSVDVLDEWNDLYQNDFSRYSSDQVLPLLTTHWHQWWPYNAY
                     YPLNPVYYKEENGHYRLGCTVTAGAQMMKYWNYPESGNGVISCENDPVTGLNYHDLSN
                     DHFRWYNMPDFLNTDTSLTEDVYGPSALVNYSFWMTLQNLNGELYCNWISSWARYFDY
                     S"
BASE COUNT          533 a          339 c          352 g          534 t
ORIGIN      
        1 aatacccctt atggaggttt tggtttgtgg cttaccccgc gcgacctggc caaagttggg
       61 ctaatgttgc tgaacaacgg gcaatataac gatcaccaaa tcctttcagc agattacatc
      121 aaacaagcaa caacggccaa aactatcatc aacgagaaag attacggtta cctgttttgg
      181 gtcgatcatt cgggaagata tttctggacc tggggtgcag gcggacagtt tatctttgtt
      241 gttcccgaaa aagaacttgt agttgttatt acttcggaac aatttgccga tgagcagaat
      301 acaacctttg agcaggctag ttatctggta gagaagatta tggaaggagt taaaaattga
      361 aacagcaacg tagcgtgttc ctaataaaca aatccttttt atagtctgta attgccttca
      421 aagatgcgaa tgctgattgg caataagtcg ttgaacttga tagtattcaa tcggtatatt
      481 tcgtagtaat attttaatca aatacatgat acagatatca aaaatttgaa tagatttggt
      541 tgataaaact caactaaatc ttgttcaaat ctctgcttat gaaatcgata ttaatcggga
      601 tacctactag ttgtagaact attctccttg gtgcaattct gttattgatg ggctcttcga
      661 cgtttgctat ttcagtaacg tttcgtgtcg atatgaccca ttttattaca atgggttttt
      721 tcaaaccagg cgtggatacg gttttggtag ttggcgattt caacaactgg gcaatcaata
      781 aacaattagc acgcgttggt tcaaccaata tttattcaac ttcgcttacc gtcaactcca
      841 attcaacata tgagtacaag ttttactgta cgggtacttc ttcctacaac gatactcata
      901 ttgacagttt tgaaggactt gtaggtgatg gcaaaaatgg aaaccgaatt ttgcaaacgg
      961 gcactaccga cattacttta cccacagtgt tttacagtaa tttcaaaccc ggaaatgcaa
     1021 ttgcaaaaca gaaagcggaa accattgcca aaaattggta taaacactgg aatcccgatc
     1081 caaaagcgac ctctgaaatt tcagaatata tctatgaaga acagtttaaa caaccttttg
     1141 cccatatttt taatttcagt tcaggaggat gggttgttgt ctctgcaacc gatcgtttga
     1201 gaccaattat gtgttacaac tttataaaca gggctcaata tatgggggct aatgcatata
     1261 acagcctcat tagcctagaa acttcggtcg acgtacttga tgaatggaat gatttatacc
     1321 agaacgattt ttctcgttac agttccgatc aggttttacc acttctgaca acccactggc
     1381 accagtggtg gccctataac gcatattatc cgctgaaccc ggtttattat aaagaagaaa
     1441 atgggcatta ccggttgggt tgtaccgtaa cagccggggc acaaatgatg aaatactgga
     1501 actatcccga atcgggaaac ggagttatca gttgtgaaaa tgatcctgta actggtctca
     1561 actatcatga tttgagcaat gatcattttc gatggtacaa catgcccgat tttcttaata
     1621 ccgacacatc attaaccgaa gatgtttatg ggccatcggc tttggtcaat tatagctttt
     1681 ggatgacact tcaaaaccta aatggagaac tttattgcaa ctggatatcg tcgtgggctc
     1741 gttattttga ttattccc
//
LOCUS       DAOPUW010000458         1758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_74889_length_1758_cov_2.183206, whole genome shotgun sequence.
ACCESSION   DAOPUW010000458 DAOPUW010000000
VERSION     DAOPUW010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74889_length_1758_cov_2.183206"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PKJ43_07665"
     CDS             <1..409
                     /locus_tag="PKJ43_07665"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNZ72482.1"
                     /translation="APYFDLDEEYAVQQAVLELISKNLIQSANDVSDGGLFITLVESA
                     IPRQLGFDITTDAEIRTDAFLFGEAQGRIVVSVSPSRETQFIDHMIAKGIPFTTLGHV
                     TKSEVRIDDESYGFIADIKREYENAFTTLMEGK"
     gene            447..1157
                     /gene="ubiE"
                     /locus_tag="PKJ43_07670"
     CDS             447..1157
                     /gene="ubiE"
                     /locus_tag="PKJ43_07670"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNZ72483.1"
                     /translation="MTAKSGNQSVEEMFNGIAHRYDLLNHILSFGVHILWRNRLVKSV
                     LKINPTKVLDVATGTADVAIALAKRDRRVEIVGVDVAEKMLSVGRAKVQKLGLDHRIV
                     LSQASAENLPFDDSTFSAVTVAYGVRNFNDLQRGLTMMYRVLESGGSIHILEFVKPKL
                     MVMRLVFGLYFHRILPLIGRCISDHSNAYSYLPQSVQTFAERDDFVKMLLAAGFVSPG
                     YSIQSFGIAAIYTAKKST"
     gene            1288..>1758
                     /locus_tag="PKJ43_07675"
     CDS             1288..>1758
                     /locus_tag="PKJ43_07675"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNZ72484.1"
                     /translation="MKKIFIILIGIAIALSLPLQMNAQYKPPTLLNDPNYDQEKWLRF
                     GFSLSLHVMDFQSLSNPIQGETEKYFVDVSHLIPGFNVNVVSDLAFTPTWHLRFLPGL
                     AFGQRNLIFYRPNGTIAHTMKMESSFIELPLTLKYSAVRVSNVRPYLIAGGNFRI"
BASE COUNT          508 a          364 c          387 g          499 t
ORIGIN      
        1 ggctccatac ttcgatctag acgaagaata cgctgtacag caagccgttc tcgaactgat
       61 aagcaaaaac cttatacaat cggcaaatga cgtatccgac ggagggttat ttataacgct
      121 tgtggaaagt gccatcccac gacaactggg atttgatatt accacggatg cggaaattag
      181 aaccgatgct ttcctttttg gggaagccca agggagaata gtggtttcgg tttcgccttc
      241 aagagaaact cagtttattg atcatatgat agcgaaaggg ataccgttta ccactcttgg
      301 acatgttacc aaatcggaag taaggattga cgatgagtcg tacggtttta ttgccgatat
      361 caaacgagaa tatgagaatg ccttcaccac attgatggag ggcaagtaaa cgcatcaata
      421 ttaccattcc attgttgttt taaggcgtga cagcaaagtc aggaaatcag agcgtggagg
      481 aaatgttcaa tggcattgcc catcgctacg atttgcttaa ccatatcctt agcttcggag
      541 ttcatatcct ttggcgtaat cggctggtaa aaagcgtatt aaaaatcaac cctacaaaag
      601 tacttgatgt tgccacaggt acagccgacg tggccatagc gcttgcaaaa agggataggc
      661 gtgttgaaat tgttggggtt gatgttgccg aaaaaatgtt atcggttggt agagcaaaag
      721 tacagaagtt ggggctcgac cacagaatcg ttttatcaca agccagtgcc gaaaaccttc
      781 ctttcgacga ttcaacattc agcgctgtaa ccgtggccta tggcgtacgt aattttaacg
      841 atcttcaacg gggccttacc atgatgtaca gagtgcttga atcgggaggg agcatccata
      901 tccttgagtt tgtcaaaccg aagcttatgg taatgcgcct ggtctttggt ttatactttc
      961 ataggatatt gccactgatt gggcgatgca tatcggatca ttccaatgca tactcatacc
     1021 taccccaatc ggttcaaact tttgccgaac gcgatgattt tgtcaaaatg ctgttagcgg
     1081 caggattcgt ttcaccaggg tattccatac aaagttttgg gatagcggca atctacaccg
     1141 caaaaaaatc aacttaattt taaaatgact atctttcagc gcgaaaaggc atcagtaagc
     1201 gcattctaaa tcgtaaaacc atgcttggtt tagcatacta aagccgaacg aagatcgttt
     1261 tttaccatgt gtttaaagtt taaaaaattg aaaaaaatat tcataatact gataggcatt
     1321 gccatagccc tttcattgcc cttgcaaatg aatgcgcagt acaagccgcc taccctgctt
     1381 aatgacccta attacgatca agagaaatgg ttgcgatttg gtttttcatt aagccttcac
     1441 gttatggatt tccaatcact gagcaatccg attcaggggg aaactgaaaa atattttgtt
     1501 gatgtgagcc atcttatacc ggggttcaac gtgaatgtgg tatctgattt ggcttttaca
     1561 cccacatggc atttacgttt cttgccgggt ttggcctttg gacaaaggaa tctaatcttt
     1621 taccggccca acggtaccat tgcacacacc atgaaaatgg aatcgtcatt cattgaattg
     1681 cctttaacat tgaaatattc tgctgttcgg gtatccaacg tacgacccta tctgattgcc
     1741 ggtggcaatt tcaggatt
//
LOCUS       DAOPUW010000459         1755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_75090_length_1755_cov_2.130588, whole genome shotgun sequence.
ACCESSION   DAOPUW010000459 DAOPUW010000000
VERSION     DAOPUW010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75090_length_1755_cov_2.130588"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..521)
                     /gene="cas2"
                     /locus_tag="PKJ43_07680"
     CDS             complement(216..521)
                     /gene="cas2"
                     /locus_tag="PKJ43_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNZ72485.1"
                     /translation="MWVLVFFDLPTETKAQQKAATRFRKNLLEDGFSMFQFSIYMRNC
                     PSRENAEVHVKRVKLNLPEYGHVGVLTITDKQFGMMELFFSKKAVDRPPAVQQLELF"
     gene            complement(690..1589)
                     /gene="cas1"
                     /locus_tag="PKJ43_07685"
     CDS             complement(690..1589)
                     /gene="cas1"
                     /locus_tag="PKJ43_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HNZ72486.1"
                     /translation="MIKRTLYFGNPAYLHKNLQQLKVIKPDDNSELGSVPIEDIGAVL
                     LDHPQITITHALMAALLERNVAIISCDEKHLPVGLMLPLDGNTIQSERFTIQIEASEP
                     LKKNLWSQTIKAKIENQATVLQKAGIDNKRILALVPQIQSGDPDNVEGRAASVYWKLL
                     FEDLPFVRDRFGTMPNAHLNYGYAILRAIVARALVGSGLLPTLGIHHSNKYNAYCLAD
                     DIMEPYRPFCDWLVYQMIKKGEITGDEITREHKATLLGIASVDIQIDGKKSPLMVGMP
                     RTSNSLFECFAGSRRKIIYPEFL"
BASE COUNT          475 a          409 c          362 g          509 t
ORIGIN      
        1 cttgcagcgg ttgtgaattg ctttcaaatt gtatttttac tgtggcttaa gacaacctct
       61 tagccgcaaa ttgcaaacaa acaagacgtt gacaaagcct acagaaaaca aaaaccaggg
      121 gcaaaagctc ccggtttttg ttttatcgct tcctttcttc atccaaaaaa cttcctgttt
      181 ttagaacttt cccttgaata attcgatcaa attcgttaaa aaagctccaa ctgttgcaca
      241 gcaggtggcc ggtctacagc ctttttactg aaaaagagtt ccatcatccc aaactgttta
      301 tcggtaatgg ttagtactcc tacatgtcca tactcgggca aattgagctt cacccgcttt
      361 acatgtacct cggcattttc acggctggga caattgcgca tataaataga aaactgaaac
      421 attgaaaaac catcttccaa caagtttttg cggaaacggg ttgccgcctt ttgctgcgct
      481 ttggtttcgg tcggcaaatc gaagaagacc agtacccaca taatccgata ttgatttagt
      541 gcatcaaaag acataattca aattgacaat tgacagttga taattgtcaa ttgatggcgc
      601 cattttcgcc ctttcagctt aaacaaacaa cattaaattc aattgttttc aactgttttc
      661 tattgctttc cattaccttc aaccaccatc tacagaaact caggatagat gatctttcgg
      721 cgtgaaccgg caaaacactc aaacaacgaa ttggaagttc gcggcatccc aaccatcaaa
      781 gggcttttct tcccatcgat ctgaatatcg accgatgcaa tgcccagtaa cgtagcctta
      841 tgctcccggg tgatttcatc gcctgtaatc tcgccttttt taatcatttg ataaaccaac
      901 caatcgcaaa acggacggta tggttccata atatcgtcgg ccagacagta ggcgttatat
      961 ttattgctgt gatgaattcc cagcgttggc aacaatccgc ttccgaccag tgcacgcgcc
     1021 acaatagccc ggagaatagc atagccgtaa ttcaaatggg cgttgggcat ggtaccaaaa
     1081 cggtcacgca caaaaggaag gtcttcaaaa agcagtttcc agtaaaccga cgcagcgcgg
     1141 ccttccacat tgtcgggatc tccgctctga atttgaggga caagcgccaa tatccgtttg
     1201 ttatcgatcc cggctttctg aagtacagtc gcctgatttt caatttttgc cttaatcgtt
     1261 tgtgaccaaa ggtttttctt cagcggttcc gatgcttcaa tctgaatggt gaaacgttcg
     1321 ctttggatgg tgtttccatc gagcggcaac attaaaccaa caggcaggtg tttttcgtcg
     1381 cagctgataa tggccacgtt acgttcgagc aaggccgcca tcagggcatg ggtgatagtg
     1441 atttgcggat gatcgagcaa cactgcacca atgtcttcaa taggtacaga ccccagttcg
     1501 ctgttatcgt cgggtttaat gacctttaac tgctgaaggt tcttgtgcag gtaggcaggg
     1561 tttccaaagt agagggtacg tttgatcatg tttcaactat ttcaggttga tcgtcgattt
     1621 gaaatacggt taggttttgt tggtgctttt caatgaaaag ttcccttccg ccaaaggtct
     1681 tcagcagctt ttctacctca ataagttcgc tacggttatg gataatttcg aagtacgaac
     1741 agtagtccca ctctt
//
LOCUS       DAOPUW010000460         1750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_75381_length_1750_cov_2.469027, whole genome shotgun sequence.
ACCESSION   DAOPUW010000460 DAOPUW010000000
VERSION     DAOPUW010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75381_length_1750_cov_2.469027"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PKJ43_07690"
     CDS             <1..296
                     /locus_tag="PKJ43_07690"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNZ72487.1"
                     /translation="LLMKECPQLHKVSEYADKLFVTPQNLNVVCRKQSDKSAGEIVTA
                     QLMLEAKRYLLHTDHTVGEISEIMNFNDTSNFVKFFKKHEKITPAQFRAQYFH"
     gene            379..1326
                     /locus_tag="PKJ43_07695"
     CDS             379..1326
                     /locus_tag="PKJ43_07695"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HNZ72488.1"
                     /translation="MNTLQSILLFILGIALVLYFAEKLVKGTVGISLGFGISTFIISV
                     IFIGFDPENLAVGAVGSYEGASGIALGSIIGAAMVAIALAFGLTALIAPMKFEKAPKR
                     ILAIPVLAVLLLGVLGLDGQLSRIDGVVLLLGFVLSIIYLIRLSKKGLDIQPTGEVAE
                     TLEDAGKLNKWKSLGIFILSLAGVVLGSELLVRSSQSIIAKMGLSDTVFGMTILAFLI
                     SIEELARELPAAIKGRPEISYGNVIGSILAFFLFNAGIIALVKPITVDPETLKYYLPI
                     CLVTTIIVSIFMSTKKVSRLAGAVLVLLYVVFAAVGFII"
     gene            1331..>1750
                     /locus_tag="PKJ43_07700"
     CDS             1331..>1750
                     /locus_tag="PKJ43_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNZ72489.1"
                     /translation="MKAKKIIQSFILTTVSDNRSLLPRLIVGLIFLSEGIQKFLFPEL
                     LGTGRFEKIGFIDPAFWAYFAGTFEIICGTFILIGLFTRIAAIPLLIIMLTAFITTKW
                     PILINRGFWAMAHEYRTDFAMTLLLIYLFIYGAGKWSI"
BASE COUNT          481 a          331 c          361 g          577 t
ORIGIN      
        1 ctcttttgat gaaagaatgt ccgcagctac ataaagtaag tgaatatgcc gacaaactgt
       61 ttgtaacacc ccaaaatctg aatgtagttt gccgaaaaca atccgataaa agtgcgggag
      121 aaattgttac tgctcagctt atgcttgaag caaagcgcta tttactccac accgatcata
      181 cggtaggcga aatttcggaa atcatgaact ttaatgatac atccaatttt gtgaaattct
      241 tcaaaaagca cgaaaaaatt actcctgctc aatttcgtgc ccaatatttt cattaatacc
      301 attttttcat ttcatattac cacatcattt ctacgaacga gaactacttt tgtaatgtaa
      361 atatttaaat tcaataatat gaatacatta caatctatct tattgtttat tctcggcatt
      421 gctcttgtgt tatattttgc cgagaaactg gtgaaaggaa cagttggcat ttcattggga
      481 tttgggatat ccacctttat catcagcgtg atttttatcg gtttcgatcc agaaaatttg
      541 gcggttggag ctgttggttc ttacgaagga gcttcgggaa tagctttagg ctcgattatt
      601 ggagcagcga tggttgctat tgctttggca ttcggattaa cggcattgat tgctcccatg
      661 aaatttgaaa aggcgcccaa acgaattctg gcaattcccg tgctggctgt tctccttctg
      721 ggcgttctcg gtttggatgg acaattgtca agaattgatg gagttgtctt gctgttgggc
      781 tttgttttgt caataatcta tctgatacga ctatcgaaaa agggactgga tattcaacca
      841 acgggagaag ttgccgaaac gcttgaggat gccgggaagt tgaacaagtg gaaatcactc
      901 ggaattttta ttctatcgct tgccggtgtg gttttgggaa gcgaactgct ggtcaggagt
      961 tctcaaagca tcattgccaa aatgggactt tcggatacgg ttttcggcat gacgatactt
     1021 gctttcctta tcagcatcga ggaactggca agggaacttc ctgctgccat aaagggacgt
     1081 cccgaaatca gctacggtaa tgtaatcgga tcgatactcg ctttttttct gttcaatgcc
     1141 ggaatcatcg cattggtaaa acccataacg gtcgatcctg aaacactgaa atattatctt
     1201 ccgatctgtc ttgttactac gataatcgtt tccatattca tgtccacaaa aaaagtatct
     1261 cgcttggccg gggctgtatt agtacttttg tatgttgtat tcgcagcagt tggatttatt
     1321 atttgatcat atgaaagcta aaaaaatcat tcaatcattt atcctaacaa cagtctccga
     1381 taatagatca cttttaccga gactaattgt cggattgatt ttcctttccg aaggtattca
     1441 gaaatttttg ttccccgaat tgcttgggac aggtcggttt gaaaaaattg gtttcattga
     1501 ccctgctttc tgggcttatt ttgcagggac atttgaaata atatgtggta cttttatcct
     1561 tattgggctg ttcacgagaa ttgcagccat accattacta attatcatgt tgaccgcctt
     1621 tattacaacc aaatggccta tccttataaa cagaggtttt tgggcaatgg cacatgaata
     1681 ccgtactgat tttgcaatga cattattact gatttacctg tttatttatg gggcaggtaa
     1741 atggtctatt
//
LOCUS       DAOPUW010000461         1746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_75593_length_1746_cov_4.432880, whole genome shotgun sequence.
ACCESSION   DAOPUW010000461 DAOPUW010000000
VERSION     DAOPUW010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75593_length_1746_cov_4.432880"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PKJ43_07705"
     CDS             <1..754
                     /locus_tag="PKJ43_07705"
                     /inference="COORDINATES: protein motif:HMM:NF025625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNZ72490.1"
                     /translation="YFLFSKGDGDKPLVYVGQSGTRKNGEGILNRLKEHHASPKQGLE
                     DWYEAVAFTTSNNIFGATEISWLENRFFALALTANRYEAKNGNEPNAGNVTEEKESEL
                     EEYVEYAKIVMGVLGHLVFEPLVQNRAIHNTDHEEDDDNLEFFMEQKMQNTGMTVKAK
                     CKRSSEGYIVLRGSVINSKTNEKTCSGVAKKAREKAKVDGNYTLLEDVLFSSPSAASM
                     FVTGASSNGYTAWKTADGKTLKDVETSEADEL"
     gene            806..1672
                     /locus_tag="PKJ43_07710"
     CDS             806..1672
                     /locus_tag="PKJ43_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72491.1"
                     /translation="MQYCRRVNMPVTAKQILDARFPGKPQPYINSTINDLVQNKKLVR
                     NDEVRPYTVRLPMDGEVIPEPKDYSRNNRSVSTKTWSSGIEVPFTRFTKEELDETAKR
                     VNRDPRYGEEGAIINSCLNKFPQNVDSDIIAMKIALIDMTNSTNLNKHLSKIYLTRLI
                     EKIKSCNFDERVAKGDISLVGELAKNEINLFSFFSKYCLYHNYYVYQNDDYVIFDGVM
                     QNNVGQFLSHYEYKGKIYKASQIKNCIEKMRQTYDYEGYLALIDSILERNNIHSKGRH
                     RKFDWFVWYNNR"
BASE COUNT          595 a          299 c          388 g          464 t
ORIGIN      
        1 ttactttctt tttagtaaag gagatggtga caagccatta gtttatgtag gtcaatctgg
       61 aactcgaaaa aatggagaag gtattttgaa ccgtttaaaa gaacatcacg cctccccgaa
      121 acaaggcctt gaagattggt atgaagctgt agctttcacg acttcaaata acatttttgg
      181 tgcaaccgag attagctggc ttgaaaatcg attctttgca cttgcattaa ctgccaatcg
      241 ttacgaagct aagaacggaa atgaaccaaa tgccggaaat gttacagaag aaaaagagag
      301 cgaacttgaa gagtatgtgg agtatgcaaa aattgtgatg ggtgtacttg gacatttggt
      361 attcgagcca ttagttcaaa atcgagcaat ccataataca gatcatgaag aggacgacga
      421 caaccttgag ttttttatgg agcaaaaaat gcagaacacc ggcatgacag taaaagcgaa
      481 atgcaaacgt tcaagtgaag gatatatcgt tttacgtggc agtgttatca attctaaaac
      541 caatgaaaag acttgctcag gggtagcaaa aaaggctcgt gaaaaggcca aggtagatgg
      601 aaattatacg cttttagaag acgtgctctt ttcttcacca tctgctgctt cgatgtttgt
      661 aacgggtgca agttcaaacg gatatactgc ttggaaaacg gctgatggca aaaccttaaa
      721 ggatgttgag acaagtgagg cggatgaatt ataaaaaacc acatgtaagt aggaggtttt
      781 agacttgagc aacgcagaaa ccattatgca atactgccgt agggtcaata tgccggtaac
      841 ggcaaaacaa atccttgatg cccgtttccc tgggaagccg cagccttata taaactccac
      901 gatcaatgat cttgttcaaa ataaaaaact cgttcgcaat gatgaagtca gaccatacac
      961 agttcggctc cctatggacg gcgaagttat tcccgaaccg aaggattatt caaggaataa
     1021 ccgaagtgtg tcgaccaaaa cgtggagtag tggtatcgag gtgcctttta cacgatttac
     1081 aaaggaagaa ctggacgaga ccgccaaacg tgtaaacaga gatccgcggt atggtgaaga
     1141 aggtgcaatt ataaatagct gtcttaataa gtttcctcaa aatgtggaca gtgacattat
     1201 tgcaatgaaa attgccttga ttgatatgac gaactccact aacctaaata aacatttgag
     1261 caagatttat ttgacaagac tgattgaaaa aataaaaagc tgcaattttg acgaacgggt
     1321 ggctaagggc gatatttctt tagttggcga attggcgaag aatgaaatta atcttttttc
     1381 attcttcagc aagtattgct tgtaccataa ttattatgtt taccaaaatg acgactatgt
     1441 aatattcgat ggtgtcatgc aaaacaatgt cggtcagttt ctttctcatt acgagtataa
     1501 aggcaaaata tacaaagcat cacaaatcaa aaactgtatt gaaaaaatgc gacaaactta
     1561 tgactatgag ggatatttag cgctgataga ttcaatcctt gagcgaaaca atatacatag
     1621 caaaggtcga cacagaaaat ttgactggtt tgtgtggtat aacaaccgat agtccaatgg
     1681 ggcaactatt aggggtgtgc aaaagcggtg catcttttaa cgatttgatt tgagcggtgc
     1741 atttta
//
LOCUS       DAOPUW010000462         1739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_76018_length_1739_cov_5.493468, whole genome shotgun sequence.
ACCESSION   DAOPUW010000462 DAOPUW010000000
VERSION     DAOPUW010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76018_length_1739_cov_5.493468"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="PKJ43_07715"
     CDS             <1..952
                     /locus_tag="PKJ43_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ72492.1"
                     /translation="VVAPEDLPFAIQRIKEVFNGADTTPKELKISKRNGEIFPVILQA
                     EVIMDEGYCSGMRGIGIDISEIKEAEEQIHLKNQQLKKINAEKDKLLSIIAHDLRSPF
                     NSFIGFTELLTEEVQTLSPAKLEEIAKSMKESATNLYALLENLLEWSKTQREGTAFNP
                     QKIKLHEEVCHCLELVGYPASKKNISFDINIPQKFDIMADRPMLDTIIRNLISNAIKF
                     TRKNGKVEISAHEENSGILIRVKDNGIGMSNELIDRLFQIDNMANRPGTDGEPSTGLG
                     LAICKELVEKHQGKIWATSQVNKGSVFYFFIPNTFSEAEN"
     gene            complement(949..>1739)
                     /locus_tag="PKJ43_07720"
     CDS             complement(949..>1739)
                     /locus_tag="PKJ43_07720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ72493.1"
                     /translation="GSIISFVAVKEDITEKRRMLAEPIEAKVKAEESDRLKSSFLANM
                     SHEIRTPLNSILGFSNFLTSDDTLTQREKQEYSDIITKSADSLLQIINDIIDISSLET
                     GQLKTWVKPVEVGPILRSLFLIFSNKLNEMNKSHLILELQLETDVTVMADENRFIQIV
                     TNLANNAMKFTERGGVRFGIESIEKDHVVFFVSDTGIGIPSNMHQSVFDRFRQVENDR
                     ARLFGGNGLGLAIVKNLVELMDGRIWLESEVGKGSIFRFTLPLD"
BASE COUNT          576 a          372 c          336 g          455 t
ORIGIN      
        1 ggttgtggca cctgaagacc ttccgttcgc gatacaacgc ataaaagaag tattcaacgg
       61 cgctgatacc acgcccaaag aattaaaaat ttcaaaacga aatggcgaaa ttttccctgt
      121 aatattgcag gcagaagtta ttatggacga agggtactgc tctggcatga gaggaattgg
      181 aattgatatt tcagaaataa aagaagctga agaacagatt catttaaaaa atcaacaact
      241 caaaaaaatc aatgctgaaa aagataaact actttcgata atagcccacg atttacgtag
      301 ccccttcaac agttttattg gtttcactga gctattaaca gaagaggtac aaactctatc
      361 cccagcaaaa ttggaagaaa ttgccaaaag catgaaagaa tcggcaacga acttgtacgc
      421 gttgctcgaa aacctgctcg agtggtcgaa aacacagagg gaagggactg catttaaccc
      481 gcaaaaaatt aaactgcatg aagaagtctg ccattgtctt gaactcgtag gatacccagc
      541 ttcaaaaaag aatatttcat ttgatataaa catacctcaa aagtttgaca ttatggccga
      601 caggcctatg ctcgatacaa tcatccgaaa cctaatatcg aacgcgatta aatttacccg
      661 caagaacggt aaggtagaaa tctctgccca cgaggaaaat tcaggaattc taatccgagt
      721 aaaagacaac ggcattggca tgagcaacga actgatagac agactttttc aaattgacaa
      781 tatggcaaat cgccctggaa ccgatggaga accaagcacc ggattgggat tagcaatatg
      841 caaagagctc gttgaaaaac accaagggaa gatttgggcc accagtcagg ttaacaaagg
      901 aagtgtgttc tactttttca tacccaacac cttcagcgag gcagagaact aatccagagg
      961 cagagtaaat ctaaatattg agcccttccc aacttcagat tctaaccaaa ttctgccatc
     1021 cattaattct accaaatttt tcactatggc taacccaaga ccattgcccc cgaataagcg
     1081 ggctctgtcg ttttcaacct gacggaagcg atcaaatacc gattggtgca tattcgaagg
     1141 aatgccaatc cctgtgtcgg aaacaaaaaa tacaacatga tctttttcaa tactttcaat
     1201 accaaacctc acgccacccc tttcggtaaa cttcatggcg ttgttggcca aatttgttac
     1261 aatttgaata aaacggtttt catcggccat aacagttaca tcggtctcga gttgtagttc
     1321 aagaatcaaa tgtgacttgt tcatttcgtt caatttatta ctgaaaataa ggaaaagcga
     1381 acgtaaaatt ggaccgacct caaccggttt cacccatgtt ttcaactgac cggtttccag
     1441 actagaaata tcgattatat cgttgataat ttgcaacagg ctatcggcac tcttggtaat
     1501 aatatctgaa tattcttgtt tttcgcgttg agtgagggta tcgtcactcg taaggaaatt
     1561 gctgaagccg agaattgaat tgagtggtgt tctaatttcg tggctcatgt tagccaaaaa
     1621 cgacgatttt aaccggtcgc tctcttctgc tttaacctta gcttcaatag gctcggccag
     1681 cattcgcctt ttttcagtaa tatcttcttt aacagccaca aaggaaatga tgctccctg
//
LOCUS       DAOPUW010000463         1739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_76034_length_1739_cov_3.490499, whole genome shotgun sequence.
ACCESSION   DAOPUW010000463 DAOPUW010000000
VERSION     DAOPUW010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76034_length_1739_cov_3.490499"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PKJ43_07725"
     CDS             complement(<1..221)
                     /locus_tag="PKJ43_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ72494.1"
                     /translation="MVKGANVEDITIARMVKKKKLRLAVLLGNRDVSCRMYTEYNEAI
                     NGFARNIHQYFGGSRCWMFFFVLISWIRVP"
     gene            complement(240..812)
                     /locus_tag="PKJ43_07730"
     CDS             complement(240..812)
                     /locus_tag="PKJ43_07730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ72495.1"
                     /translation="MLSKPFLPKTNSGDYLPKVSVLIPARNEEANLPNLLSDLSVSVY
                     PNLEVIVCNDHSTDGTSKILSDFQEANSNFNWFLGEKLPEGWVGKNYACHQLAQRATG
                     DYLLYLDADVRVSALLINYLVSFAYRRKLALLSVFPQQQIISNGEWCTVPIMNWILLS
                     FLPLPLVRMRWFPSLSAANGQVMFFETKSY"
     gene            complement(875..1498)
                     /locus_tag="PKJ43_07735"
     CDS             complement(875..1498)
                     /locus_tag="PKJ43_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNZ72496.1"
                     /translation="MITAQHNSLVTCFFNWYILRIMRNDFHSITVSGKWESKPAGSLV
                     IGNHFSWWDGFFGLYLNNRFLKKHFFVMMLESQLQRRKMFSKAGAFSVNPGSRSIVES
                     LNYAACLLESKENMVLIFPQGILHSLYNTRFSFASGVEKILRKTPSCQLLFYVAMVDY
                     FSNRKPSLFFYLKEVNSDEDDFSIENVENLYNTFYAECLEKHAQRTE"
     gene            complement(1495..>1739)
                     /locus_tag="PKJ43_07740"
     CDS             complement(1495..>1739)
                     /locus_tag="PKJ43_07740"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HNZ72497.1"
                     /translation="LGALCMTVYDFVMEPVAMKTDMWSWVFTKIPMQNYVVWFMVAAL
                     VISAFELFQIKTENRIAGRIFIAQFVFFAILNLFLR"
BASE COUNT          600 a          383 c          283 g          473 t
ORIGIN      
        1 ggaacacgta tccatgagat aaggacaaaa aagaacatcc aacacctgct gcctccaaaa
       61 tattgatgaa tatttcgggc aaagccatta attgcctcgt tatattcagt atacattcga
      121 caagaaacat ctctatttcc taacaaaact gccagtctta attttttctt tttcaccata
      181 cgggcaatgg tgatatcctc aacattagct cctttcacca tctgatgcca taaatgttcc
      241 taataacttt ttgtttcgaa gaacataacc tgcccgttag cagccgaaag tgagggaaac
      301 catctcattc gcacaagcgg caagggcaaa aatgaaagca aaatccagtt cataatcgga
      361 acagtacacc attctccgtt tgaaataatt tgctgctgag gaaaaacaga aagcaatgcc
      421 aacttccttc gataggcaaa agaaactaag taatttatta acaaagcact aactcgcaca
      481 tcagcatcga gatacagcaa ataatcacct gtggctcttt gagccaattg gtggcaagca
      541 taattcttac ccacccaacc ttctggcaat ttttccccta aaaaccagtt gaaattactg
      601 tttgcctctt ggaaatcaga caatatttta gaggtcccat cggttgaatg gtcgttgcat
      661 acaataactt ccagattagg ataaacacta accgataggt cggaaagcaa attgggaagg
      721 ttagcttctt catttcgggc aggaatcaac actgaaactt ttggcaaata atctccggag
      781 ttcgtctttg gcaagaatgg cttcgataac atattggtta ataccaccat gaatcgcagc
      841 atcaacatta tcaacaatat gtatcctata tagatcattc ggttcgttgt gcatgttttt
      901 ccaaacactc agcataaaaa gtattgtaga gattctcaac attttctatt gaaaaatcat
      961 cttcgtcgct attcacttcc ttcagataga aaaataagct tggttttctg tttgaaaaat
     1021 aatcgaccat ggcaacataa aacaacaatt gacacgaggg tgttttacgc aaaatctttt
     1081 caacaccact tgcaaacgaa aacctagtat tatataagct atgaagaatg ccttgcggaa
     1141 aaatgagaac catattttct ttgctttcca acaaacatgc agcataattt aagctttcaa
     1201 caatacttcg cgaacccggg tttaccgaaa aagcgccagc ctttgaaaac atttttcgcc
     1261 gctgaagctg agattccaac atcatgacaa aaaaatgttt ttttagaaaa cggttgttca
     1321 aatacagccc gaaaaaacca tcccaccaac taaaatgatt tccaataacc aaacttcccg
     1381 caggtttact ctcccacttt cccgataccg ttatcgaatg aaaatcgttg cgcataatac
     1441 gcaaaatata ccaattgaag aaacaggtaa ccaacgagtt atgttgggca gtaatcatct
     1501 tagaaagaga tttagaatag caaaaaagac aaactgagct ataaaaatcc ggccagctat
     1561 tctattttct gtttttattt ggaacaattc gaatgctgaa ataacgaggg cagctaccat
     1621 aaaccaaaca acataatttt gcatcgggat cttcgtgaac acccacgacc acatgtcggt
     1681 tttcatggct acaggttcca ttacaaaatc atagacagtc atacacaaag ccccaagta
//
LOCUS       DAOPUW010000464         1731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_76598_length_1731_cov_2.689737, whole genome shotgun sequence.
ACCESSION   DAOPUW010000464 DAOPUW010000000
VERSION     DAOPUW010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76598_length_1731_cov_2.689737"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="PKJ43_07745"
     CDS             <1..1277
                     /locus_tag="PKJ43_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNZ72498.1"
                     /translation="FHYFQFVMGNVAGYMLIALFLLPFYYRENVYSIYSVLERKMGRE
                     GYLTSSGFFIVSKVIGASFRLFLAILVLYLAIASPLGFSFAGTVLLCLLLIWLYTFRS
                     GIKTVVWSDTVQTVMLIAAAVITIVLLLKQLSFSATDFFSMLKSDPQTRIFDFQWQSP
                     NNFFKQFLSGIFITLAMNGFDQDIVQKNLTCKSGKKARKNMLWFSVSFAITVFLFLVL
                     GALLYAFAESKGIALPSKSDQLYPLIALNYMGSVASVLFILGISAAAFSSADSATTAL
                     TTAFCIDFLKLHQRKQIVQSRVRNLVHLGFSILIFCVIMLFYKLNNESVVNAIFKAAG
                     YTYGPILGVYLFSFFVPRIPRRKLILPICVLAPLLTYGITRLLFIVMDGYVFGFELII
                     VNTLLTLALLTLFSSPKNHGHELTNSESNSFN"
     gene            complement(1328..>1731)
                     /locus_tag="PKJ43_07750"
     CDS             complement(1328..>1731)
                     /locus_tag="PKJ43_07750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNZ72499.1"
                     /translation="YRQTWDLAFFNYLKNLETQKPVIVCGDMNVAHRPIDLTHPKANY
                     NKYAGYMQEEIDGMDRFTQGGLVDSFRYFYPDKTGAYSWWSYRAGARAKNVGWRIDYF
                     LVSESFMTQVKSASILPEIMGSDHCPIAIEL"
BASE COUNT          415 a          342 c          366 g          608 t
ORIGIN      
        1 cctttcatta ttttcaattt gttatgggta acgtggcagg ttatatgctt atcgcccttt
       61 ttttgcttcc tttttattat cgtgaaaatg tatactcaat ttattcagtg ttggaacgaa
      121 aaatgggacg ggaaggatac ttaacaagct ctgggttctt catcgtttcg aaagtaattg
      181 gggcctcttt tcgcctgttt ttagcgattc ttgttcttta cttggcgatt gcaagtccgt
      241 taggattttc atttgccggt acggtgcttt tatgtctgtt gcttatctgg ctttatacgt
      301 tccgcagcgg aattaaaacg gttgtatggt ccgataccgt ccaaacggtg atgttaatcg
      361 cggccgctgt tattactatt gttttgttgc ttaagcaact tagtttttcg gcaactgatt
      421 ttttttctat gctgaagagt gacccgcaaa cccgaatttt cgattttcaa tggcaatcgc
      481 ccaataattt tttcaagcaa tttctatcag gaatattcat cacgctggca atgaatggtt
      541 tcgatcagga tattgtgcag aaaaacctca catgtaaatc ggggaagaaa gccagaaaga
      601 atatgttatg gttttctgta agctttgcga taactgtttt tctctttctt gttctgggtg
      661 cacttttata cgcgtttgct gaatctaaag gtattgctct gccctcgaaa agtgatcagc
      721 tttacccact tattgcattg aactatatgg gcagtgtggc atctgtttta tttattctag
      781 gtatctcggc agcggcattt tcaagtgccg actctgcaac aactgcattg actactgctt
      841 tttgcatcga ttttttaaaa cttcaccagc gcaagcaaat tgtgcagtcc cgagttagga
      901 atctggttca tcttgggttt tcaatcttga ttttttgtgt gataatgctg ttttataagc
      961 ttaataatga aagtgtagta aatgcaatat tcaaggctgc gggctacacg tatggcccaa
     1021 ttctgggagt ttatcttttt tcattttttg ttccaagaat cccgcgacga aaattaatct
     1081 taccgatatg cgttttggct ccattgttaa cttatggaat aacacgtttg ttattcattg
     1141 taatggatgg atatgttttt ggttttgaat tgattattgt aaatactctt ttgactcttg
     1201 ctttattgac gttgttctct tcgcccaaaa accatgggca cgagttaacc aacagtgaat
     1261 ctaattcgtt taattaaaaa cacttagtat aagtcgtact gcagtatagt ttcttcagtt
     1321 gtgttcatta cagctcaatg gctatcggac aatggtcgct gcccataatt tcgggcagga
     1381 tactggccga tttcacctgt gtcatgaaac tttcgctcac caaaaagtag tcgattctcc
     1441 agcccacgtt cttggcgcgt gctccggcgc gatagctcca ccacgaataa gctccggttt
     1501 tatcagggta gaaatatctg aaactatcga ccaacccacc ttgggtaaag cgatccatcc
     1561 cgtcgatttc ttcctgcatg tagcccgcgt atttattgta gttggcttta gggtgcgtga
     1621 ggtcgatggg gcggtgcgca acgttcatgt cgccgcaaac aatcacgggc ttttgtgttt
     1681 cgaggttttt caggtaattg aaaaaagcca aatcccaagt ttgccgatag t
//
LOCUS       DAOPUW010000465         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_76976_length_1726_cov_1.882705, whole genome shotgun sequence.
ACCESSION   DAOPUW010000465 DAOPUW010000000
VERSION     DAOPUW010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76976_length_1726_cov_1.882705"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PKJ43_07755"
     CDS             complement(<1..545)
                     /locus_tag="PKJ43_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72500.1"
                     /translation="MSKRVLADNIKWLIGSTLILFLLGCSSESSRKDRIEVDASEISS
                     QELAKEGIQTARQIFYSLPSPLETAMILKRSGAQYNEELLNPVDNASKYTTNKSMALN
                     LGIYSTDLSYASLFDQTQASIKYMAVSKRMAEGLGILNAIDNTIVQRLEENVNNREAI
                     MDIISESLLNTSSILEEDDRVA"
     gene            744..1313
                     /locus_tag="PKJ43_07760"
     CDS             744..1313
                     /locus_tag="PKJ43_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72501.1"
                     /translation="MLRYFKLGMENRDEKTVKERQRMIVHSLMNDSDDKIITFLHGVK
                     ENYELFIIEPLLDLLMTERSLLLKKHIVEFISNAKEKDVVSILVKHLKKNFPHKNVGD
                     MLTICWQSRLDFSGSLDVFFEILVDGDYQTSIEAFTVIESAMMNLNREEIEQRLDWLK
                     ISSHRVISNKQSLIHEMENLLDRTRSEML"
     gene            complement(1343..>1726)
                     /locus_tag="PKJ43_07765"
     CDS             complement(1343..>1726)
                     /locus_tag="PKJ43_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNZ72502.1"
                     /translation="DIDIDKLKVIPAEVFSSPTEIRDILRWVAIASHEVPEINISAST
                     GIHNGDAAIKMLLAGATTLQVCSTLYIHGASHLGKIIEFIEKWMDGKGYKSIDDFRGL
                     MSYKNIGDPEVYERSQFMRHFSAHE"
BASE COUNT          531 a          351 c          343 g          501 t
ORIGIN      
        1 gcaaccctat cgtcttcctc aagaattgaa ctggtattga gcaacgactc tgaaataata
       61 tccataatcg cttcgcgatt attaacattc tcctcaagcc tttgaacaat ggtattatca
      121 atggcattga gtattcccaa tccctcagcc atgcgcttcg atacagccat gtacttaatg
      181 gaagcctgag tttggtcaaa caagctggca tagcttaaat cggtgctata aatccccaaa
      241 ttaagggcca tgcttttgtt ggtggtgtat ttcgaggcat tgtcaacagg atttaacaac
      301 tcctcgttgt attgagctcc tgaacgcttc agaatcatgg cagtttccaa tggagagggt
      361 aaggagtaaa aaatttgacg tgccgtctga atcccctctt tagccaattc ttgcgaggat
      421 atttcggaag catccacttc tatcctatcc ttacgggacg attctgaaga gcacccaagt
      481 aaaaacagga ttagtgttga acctatcaac cattttatat tgtctgccag tacccttttc
      541 gacataacta aatttttttg aggtgtttac gtttaatacc aagttttggt tagtaaaaat
      601 aatatttttt ttcttacaat aggcaaagtt taaaattatt ccacaaaata tggccttatt
      661 tttcgaattt agcgctaaag taacgaataa ttactgagga atgaaaaaaa ccgattaaga
      721 aaaaagtgta aacagtatta cctttgttgc gttatttcaa gttgggtatg gaaaacaggg
      781 atgaaaaaac ggttaaggaa cggcaacgaa tgattgtcca ttcgttgatg aatgattcgg
      841 acgacaaaat cataaccttc ctgcatggag taaaagagaa ctacgaattg ttcattattg
      901 aaccactgct ggatctgcta atgacggaaa ggtcgttgtt gcttaaaaag catattgttg
      961 agtttatctc aaatgcaaag gaaaaggatg ttgtctcaat tcttgtaaag catctgaaaa
     1021 agaactttcc gcataaaaat gttggagaca tgctgaccat ttgttggcaa agcagactcg
     1081 atttttcggg tagcctcgat gtgttcttcg aaatactggt ggacggtgat taccaaacct
     1141 ccattgaggc attcacggtt attgaaagtg ccatgatgaa tctaaaccgg gaggagattg
     1201 aacagcgttt ggattggtta aagatctcct cgcaccgtgt aatcagcaac aaacaatcac
     1261 tcattcacga aatggaaaat ctcctcgaca ggacaagaag cgagatgctg taaaattttc
     1321 gtcagttgaa atcctatcct ccttactcgt gcgccgaaaa atgcctcata aactggctac
     1381 gctcatacac ctcaggatcg ccaatgtttt tgtaactcat taaccctcta aagtcgtcaa
     1441 tggatttgta gcccttacca tccatccatt tttcaataaa ttcaataatc tttccaaggt
     1501 gtgacgcacc atggatgtac aaagtagagc aaacctgcaa agtggttgcc ccagcaagca
     1561 acatcttaat ggctgcatcg ccattgtgga ttccggtgga agcagaaata ttaatctcag
     1621 gaacttcatg gctggcaatg gccacccagc gcaaaatgtc cctaatctca gtgggactac
     1681 tgaacacttc ggcaggaatt actttcagtt tatcgatatc gatatc
//
LOCUS       DAOPUW010000466         1721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_77309_length_1721_cov_2.996399, whole genome shotgun sequence.
ACCESSION   DAOPUW010000466 DAOPUW010000000
VERSION     DAOPUW010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77309_length_1721_cov_2.996399"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..674)
                     /locus_tag="PKJ43_07770"
     CDS             complement(192..674)
                     /locus_tag="PKJ43_07770"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HNZ72503.1"
                     /translation="MDILLIIGGIFFLLLGLAGCILPGLAGPPLSYVGLLLLHLTEKY
                     QFSTRFLIIWAVITLLVSLLENFIPIWGTKKYGGSKRAMWGSVVGLLVGLFVFPPMGI
                     IIFPFIGAVVGELSAGKGTNEALRAGFGAFMGFVIALVMKLTVSGLMIFFFVKELIVS
                     "
     gene            896..>1721
                     /locus_tag="PKJ43_07775"
     CDS             896..>1721
                     /locus_tag="PKJ43_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005416 - amino acid:monoatomic cation
                     symporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HNZ72504.1"
                     /translation="MNHFSELLQLIDSYLGGSQWFVFLLLGTGLFFTLYLKFPQIRYF
                     RYAVKIVKGKFDRQSDKGDTSHFQALTTALSGTVGTGNIAGVALAIHLGGPAALFWML
                     VTAMLGMTTKLVEVTLSHKYREITADGTVAGGPMYYMKNRMKMPWLAAIFAVATIFSS
                     FGTGSLPQINSISNSVFSTFGINKIVTGAVLSILLGFVIIGGIKRIAKVTERLVPFMA
                     IFYFVGAISVIIFNYKNFFPSLSSIFTDVFTGTAATGGFLGAGFAFAFNRGVNRGLF"
BASE COUNT          543 a          365 c          320 g          493 t
ORIGIN      
        1 gggaagaatt acctcaacaa agtttcatca ctggacatca atacattaca aagcggattc
       61 aaaatgaaac gggcaaaaga attttctggt tttatgaacc aagcaatcta tgttgttagc
      121 ttcacgagcg attacgaggt gaaaatagtt tcaaggataa cagattagac ttcaatatat
      181 tctattcgta ttcaggaaac aataagttct ttcacgaaaa agaaaatcat taagcccgaa
      241 acagttagtt tcataaccag agcaataaca aaccccatga aggccccaaa accggctcgg
      301 agagcttcat tagtcccctt gcctgccgat aattcaccta ctactgcgcc aataaatggg
      361 aaaatgataa ttcccattgg agggaaaaca aataacccga ccaacaagcc aaccacactc
      421 ccccacatgg ccctcttgct gccaccatat tttttagttc cccaaattgg gataaagttt
      481 tcgagcaaag aaacaagcaa tgtaattaca gcccaaataa ttaagaaacg agttgaaaat
      541 tgatatttct ccgtaagatg gagcaacaaa agccccacat aacttaacgg aggacctgcc
      601 aatccgggta agatacatcc ggcaagtccc aataagagga aaaagattcc accaatgata
      661 agtaatatat ccatcttttt ttggtgaaaa atacaaaatt ttactttacc tattaacctg
      721 tgttttattt taataaggaa tcacccaaag atatgattgc atagagataa gcaaatcagc
      781 catcgaaata gccaaactct caaaaatgaa gcaaaatcag ttactactgt tagttatata
      841 aagaattatt gttattttgg gcaatcaaac ttaacagcac ctttttaccc attatatgaa
      901 ccatttttcg gaactgctcc aacttatcga ctcgtacctg ggaggttctc agtggtttgt
      961 ctttttactg cttggtaccg gacttttttt caccttatat ctgaaatttc ctcagatccg
     1021 ctacttcaga tatgcggtaa aaattgttaa aggcaaattc gacagacaaa gcgacaaggg
     1081 agatacatct cattttcaag cactaacgac tgctctttcg ggaacagtgg gtacagggaa
     1141 catcgcaggc gtagctttag ccattcacct tggaggccct gccgctctct tttggatgct
     1201 cgtaacagcc atgctcggga tgacaaccaa acttgtagaa gtaacccttt cacataaata
     1261 ccgcgaaata acggcagacg gtactgtggc tggcggacca atgtattaca tgaaaaatcg
     1321 gatgaaaatg ccttggctgg cagcaatttt tgctgttgct actatttttt cttcgttcgg
     1381 tacaggaagc ctgccccaaa ttaattccat ttcaaattca gtgttttcca cctttggaat
     1441 caataaaata gtaacaggtg ctgtactctc gatacttctt ggctttgtta ttattggagg
     1501 tataaaacgg atagcaaaag tgactgaacg tttggtcccg tttatggcga tcttctattt
     1561 tgtaggcgcg atttcggtca taattttcaa ctataaaaat ttctttccct ctctgtcctc
     1621 tatttttact gatgttttca ccggtacagc agcaacagga ggttttttgg gggctggttt
     1681 cgcgtttgcc ttcaacaggg gagtaaaccg aggattattc t
//
LOCUS       DAOPUW010000467         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_77580_length_1717_cov_4.523466, whole genome shotgun sequence.
ACCESSION   DAOPUW010000467 DAOPUW010000000
VERSION     DAOPUW010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77580_length_1717_cov_4.523466"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PKJ43_07780"
     CDS             <1..561
                     /locus_tag="PKJ43_07780"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HNZ72505.1"
                     /translation="SKPRGCLLFPDDFSHRGSADSVRKALQHLKEKGLIKTVTRGIYV
                     RPVIDEYIGEVLPTAEEVAKAIARRDKIQLVPTGAYALNALGLSTQMPLKLVFLTDGA
                     PRTIKVGKRTIKLKKATPKSLKARGKISALVVQALRAIGKDKASDDELSKIIKLLKEE
                     DPADLKYDISLAPEWIKKIMKQAVNE"
     gene            554..1588
                     /locus_tag="PKJ43_07785"
     CDS             554..1588
                     /locus_tag="PKJ43_07785"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNZ72506.1"
                     /translation="MNRFYNLPAESKERILNETGEKSNLPAYAVEKDWWVVQALTILF
                     ELEIGKHLVFKGGTSLSKSWGLIERFSEDIDLAVDRTFYGFDGELTKERRTKLRKTAN
                     AYIRETLFPELQKAFAAKGLSGVAIELEKITTSDQDPVIIHVNYPNLIESPGYIQPRV
                     QIEIGCRSMIEPFENRPLLSLVDTTYPETQFSQKPVNIPSVVPERTFLEKTFLLHEEF
                     QRPPGKMRVNRLSRHLYDIYKLSQQGVADKALSGKELYETIVRHRYGFARVGDVDYNF
                     HQPKTINFIPHKEFIAAWAEDYKIMQEQMIYGASPTFDQLIGYLKALNVKINELPWEA
                     SFSFKQIKNK"
BASE COUNT          588 a          322 c          366 g          441 t
ORIGIN      
        1 tcaaagcctc gtggttgttt gttgtttccc gacgatttta gccacagggg gtcggctgat
       61 tcggtacgta aagcgctgca acacttaaag gaaaaggggc ttataaaaac cgttacccgt
      121 ggtatttatg tacgccctgt aattgatgaa tacattggcg aggtattacc aacggccgaa
      181 gaggtagcca aagccattgc caggcgcgac aaaatacagt tggttccgac aggagcttat
      241 gccttgaatg cgttgggctt aagcacacaa atgccattaa aactggtgtt tttaactgat
      301 ggggctccgc gaaccataaa agtcgggaaa agaaccatta agctaaaaaa agctactccc
      361 aaaagcttaa aggccagagg taaaataagc gccctggtgg ttcaggcgtt gcgcgcaatt
      421 ggcaaggata aagcatcgga tgacgaatta agcaagatca taaaattgct taaagaggaa
      481 gaccctgcag atttaaaata cgacatatca cttgctcccg aatggattaa gaaaataatg
      541 aaacaagccg taaatgaata gattttacaa tttacctgcg gaatcaaaag agaggattct
      601 taatgaaacc ggtgagaaaa gcaatttgcc tgcctatgcc gtagaaaaag actggtgggt
      661 ggtacaagcc ttaaccatac tgtttgaact cgaaataggc aaacatctgg tatttaaagg
      721 aggtacttct ttgagcaaat catggggttt aatagaacgg ttttcggaag atattgattt
      781 ggccgttgac cgcacttttt atggttttga tggtgaattg accaaagaaa gaagaacgaa
      841 actaaggaaa acggccaacg cgtacatcag ggaaacatta tttccggaat tacaaaaagc
      901 attcgcggca aagggtttat cgggtgttgc aatagaattg gaaaaaatta caaccagcga
      961 ccaggaccct gtgatcatac atgtgaatta tcccaacctt atcgaatcgc cgggttatat
     1021 acagccacgg gttcaaattg aaattggttg ccgttcaatg atagagcctt tcgaaaaccg
     1081 gccactttta tcgctggttg atacaactta ccccgaaact caattttctc aaaaacctgt
     1141 aaatatacct tcggttgttc cggagcgcac attcctcgaa aaaacatttc tgttgcacga
     1201 agagtttcaa agaccacctg gtaaaatgcg ggtcaatcgc ttaagccgcc atttatacga
     1261 tatttataag ctttcacaac aaggagtggc tgacaaagca ttgtccggaa aagaattata
     1321 tgaaaccatt gtcaggcacc gctatggttt tgcaagggta ggagatgttg attataattt
     1381 tcatcaaccg aaaacaatta attttatacc tcataaagaa ttcattgctg cctgggctga
     1441 agattataaa ataatgcagg aacaaatgat ttatggagcc tctcccacat ttgatcaact
     1501 gatcggatat cttaaagcgc tgaatgtgaa aataaatgaa ttgccatggg aagcctcctt
     1561 ttcattcaaa caaataaaaa ataaatgaag tttcgcataa aaaataaaat tgataaatac
     1621 caatcatttg cgaattgcaa agagattaac actatggaaa acaacttttt tacatatagt
     1681 caaaaagaac aaaactacgg tcagtcagag tagtaat
//
LOCUS       DAOPUW010000468         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_77742_length_1715_cov_2.693976, whole genome shotgun sequence.
ACCESSION   DAOPUW010000468 DAOPUW010000000
VERSION     DAOPUW010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77742_length_1715_cov_2.693976"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PKJ43_07790"
     CDS             <1..531
                     /locus_tag="PKJ43_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HNZ72507.1"
                     /translation="VPVPNIFTEFGSFTVGESGAVVYSVLHEKMQNDREIWYMIDSSF
                     ITTLPDTWGIGEKFLMLPVNKWQEDYQEVHLGGLTCDSHDFYTSEEHINAVFLPKSNT
                     VSTDPTVKSEPLYIGFFHTGAYQDQLSGYGGIKHCMIPSPKHVVIEYDKNGKLVDWLY
                     AKEQTFQSMLKILGYK"
     gene            complement(790..>1715)
                     /locus_tag="PKJ43_07795"
     CDS             complement(790..>1715)
                     /locus_tag="PKJ43_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ72508.1"
                     /translation="YIDGIKVTQVDSKATEFLLFMQGDLDFMNGIDASFKDQVLSKKG
                     ILKDEYNGKIALQKHAYLNVEYLGFLIDDEKNPQKILLNKKIRQAINYGFDRKKLVTY
                     LRNNIGIAANAGIIPISLQGFDSSVVKGYSYQPELAKKLIEEVSTVSGKLPTITLLSN
                     DNYSDRCSFIASQLSNLGLDIKIEIMQPALLREQMSNSNAPFFWATWIADYPDAESYL
                     TMFYGKNTAPPNYTRFKNETFDRLYEQSLTESDEKTKIALYQQMDRIIIEEAPCVPLF
                     YDEVMHFLNKRVSNWKTNSLNLLEIKEVKIK"
BASE COUNT          442 a          338 c          376 g          559 t
ORIGIN      
        1 gtaccggttc cgaatatctt taccgaattt ggttcgttta cagtgggcga gtcgggtgca
       61 gttgtttaca gtgtgcttca cgaaaaaatg caaaacgacc gcgagatttg gtatatgatt
      121 gacagttcat tcattaccac cttgcccgat acctggggta ttggcgaaaa gtttctgatg
      181 ctacctgtca ataaatggca ggaagattat caggaagtgc atctgggcgg actcacctgc
      241 gacagccatg atttttatac atctgaagaa catatcaatg ccgtgttttt gccaaagagt
      301 aatacggtct ccacagaccc aactgtaaaa tcagagcctt tgtatattgg tttctttcat
      361 acaggtgctt atcaggatca gttgagtggt tatggtggca tcaagcattg catgattcct
      421 tcgcccaaac atgtggtgat agagtatgat aaaaatggta agctggtaga ttggttgtat
      481 gcgaaagagc agacgtttca gagtatgctc aagattcttg gatataagta gtgttctgtt
      541 taacacgccc gattagggca gttgtgattt tcaaactttt taatcgggcg ctactcacct
      601 gcatctccaa cttctgcgtc tgtaccaaac ggttgtacca aagtactacc cgttcggcac
      661 ctccttgaat ttgtagcgct tgtgagttaa acagcaagta ctcttgatta gaatattctc
      721 ttttgcctgc ccagcggtca gacggggatt ttgtgttttg ctcgcccggc gctcggatgg
      781 ggaatttgtc tatttgattt tcacttcttt gatctccagc aaatttaaac tattggtttt
      841 ccagttgcta acccgtttgt tgagaaagtg catcacctca tcataaaaca atggcacgca
      901 gggggcctct tcaataatga ttcggtccat ttgctggtat aatgcgattt ttgttttttc
      961 atctgattcg gtcaaacttt gttcgtacaa gcggtcaaaa gtttcgtttt taaaacgggt
     1021 ataattcggt ggtgcggtat tttttccata aaacatggtg aggtaacttt ctgcatcagg
     1081 ataatctgcg atccaggtgg cccagaaaaa aggggcattt gaattgctca tttgttcgcg
     1141 caataaagcg ggttgcataa tttcgatttt aatatccaaa cccagattac tcaattgcga
     1201 agcgataaaa ctgcaacggt cagaataatt atcattcgaa aggagggtaa tagtagggag
     1261 cttgcccgat acagtgctaa cttcttcaat cagttttttt gcaagctcgg gttgatagct
     1321 ataacctttg accacactgc tgtcgaagcc ttgcaagctg atgggtatga tacccgcatt
     1381 ggcagcgatg ccgatattgt tgcgtaagta agtcactaat ttttttcggt caaaaccgta
     1441 attgatggct tgtcgtattt ttttattcag cagtattttt tgtgggttct tttcatcgtc
     1501 tattaaaaat cccaggtatt ctacatttag ataggcatgt ttttgtaggg ctattttgcc
     1561 attgtattca tctttcaaaa ttcctttttt gctcagtacc tgatctttaa aactggcgtc
     1621 aatgccattc ataaagtcaa ggtcgccctg cataaataac agaaactcgg tggctttact
     1681 atcgacctgt gtcactttaa tgccatcgat ataag
//
LOCUS       DAOPUW010000469         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_77744_length_1715_cov_2.654217, whole genome shotgun sequence.
ACCESSION   DAOPUW010000469 DAOPUW010000000
VERSION     DAOPUW010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77744_length_1715_cov_2.654217"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..489)
                     /locus_tag="PKJ43_07800"
     CDS             complement(64..489)
                     /locus_tag="PKJ43_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016779138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNZ72509.1"
                     /translation="MIIKQLSVFLENKSGRLCEVTNILGEAGINMSAFSIADTSDFGI
                     LRVIVSDPDKALQVLRNNHFSVNLTEVICLSCPNVPGAMAKALNILNEEQIPIEYMYA
                     FSMGEVANVVIKPTNVRKCIEVLQKYELDLLKSSDLYKM"
     gene            complement(500..>1715)
                     /locus_tag="PKJ43_07805"
     CDS             complement(500..>1715)
                     /locus_tag="PKJ43_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNZ72510.1"
                     /translation="RVYHDVPYYRKKMQEVGLVPEDIRTIEDIVKLPFTTKTDLRDNY
                     PFGTFAVPMSEIVRIHASSGTTGRPTVVGYTRNDIAMWAENVTRSLCMAGVTKNDLVQ
                     VAYGYGLFTGGLGIHYGTENLGASVIPISGGNTQKQIQLLQDFGSTVICCTPSYVLHI
                     AEVLEEMKIDASSLKLRVGIFGAEPWSENMRRDIEKKLKIKAIDIYGLSEVMGPGVSC
                     ECTHQAGMHINEDHFIPEIINPETLLPVEPGEVGELVFSTITKEGIPLLRYRTRDLTR
                     LIYDKCECGRTLVRMEKCKGRSDDMLIIRGVNVFPSQIESVLLEMSETEPHYLIFVER
                     EGNLDTVKVQVEVQEQFFSDEIKELQNLTKKIKYKLESTLGISVTVQLVEPKTIERSA
                     GKAKRVIDNRKI"
BASE COUNT          462 a          385 c          331 g          537 t
ORIGIN      
        1 aagaggtcta tttttgctga atatttatct gccgttaaaa aattgtatcg cttttcaggc
       61 tatttacatt ttatataaat cactggattt caacagatca agttcatact tctgcaatac
      121 ttcgatacat tttcgaacat tagttggttt tattacaacg ttcgcaactt cgcccatcga
      181 gaaagcatac atgtattcaa taggaatctg ctcttcgtta agaatattca gagcttttgc
      241 catggctccg ggaacatttg ggcaactaag gcagataact tcggtaaggt tcaccgaaaa
      301 atggttattg cgaagcacct gaagtgcttt atctggatca gaaacaatta ccctgaggat
      361 accaaaatcg ctggtatcgg caatgctgaa agctgacatg ttgatgcctg cttcgcccaa
      421 aatattagtt acctcgcaca aacgtcctga tttgttttcg aggaatacgg acagttgctt
      481 aattatcatg gcaatagtat taaatttttc tgttgtcgat tactcttttt gcttttcctg
      541 cagaccgttc aatcgtttta ggttcaacaa gttgcactgt aactgaaatt cccaatgtgc
      601 tttcgagctt gtattttatc tttttagtca ggttttgcag ttctttaatc tcgtcggaga
      661 agaactgttc ctgtacttca acctgaactt tcacagtatc gagatttcct tccctttcta
      721 caaagataag ataatgtggt tctgtttcgc tcatttcgag caatacactt tcaatttgag
      781 acgggaatac gttgacccca cgaatgatga gcatatcgtc gctgcggcct ttgcattttt
      841 ccatacgcac caacgttctt ccacattcgc atttgtcgta aataagacgg gtaaggtcgc
      901 gtgtgcggta acgcaacagc ggaattcctt ctttggtaat ggtagaaaat accaactcac
      961 ccacttcccc tggttcaaca ggcagcaaag tttcaggatt aattatttca ggaatgaagt
     1021 gatcttcatt gatatgcatt ccagcctggt gagtacattc gcacgaaacg cccggcccca
     1081 ttacttcgct cagcccataa atatcgatgg ctttaatttt tagtttcttt tcgatgtcgc
     1141 gcctcatgtt ttccgaccag ggttcggcgc caaaaatgcc cacccgaagt ttcaacgacg
     1201 aagcatctat tttcatttct tccaatactt cagcaatatg aagcacgtat gaaggagtgc
     1261 agcatataac agttgaaccg aaatcttgaa gcaactgaat ttgcttttgg gtatttcctc
     1321 ctgaaattgg aattacgctt gccccaaggt tctcggttcc ataatgaatc ccaagtcctc
     1381 ctgtgaagag cccataacca taggctacct gaaccaaatc gtttttggtt acaccagcca
     1441 tacacaacga gcgggtcaca ttttcggccc acatagcaat gtcgttacgg gtatatccta
     1501 caactgttgg ccgtcccgta gttcccgaag aggcatgaat acgtacaatt tctgacattg
     1561 gaacggcaaa agtaccgaac ggataattgt cacgaagatc ggttttggta gtaaatggaa
     1621 gctttacaat atcttctata gttcggatat cttcaggcac cagccctact tcctgcattt
     1681 ttttcctata atacggtacg tcgtgatata cacgt
//
LOCUS       DAOPUW010000470         1714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_77793_length_1714_cov_3.410488, whole genome shotgun sequence.
ACCESSION   DAOPUW010000470 DAOPUW010000000
VERSION     DAOPUW010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77793_length_1714_cov_3.410488"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PKJ43_07810"
     CDS             <1..711
                     /locus_tag="PKJ43_07810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ72511.1"
                     /translation="VEDVEDGIQGLEKIKNSDHRIKVFEYNVPFNFSAINNWAIEQCE
                     GEYILLLNNDIKAISDGWLNAMVEHIQLENVGIVGAKLLYSDDTIQHAGVIIGIQGVA
                     GHSHKFCPDHSVGYFYRPVVIQELSAVTGACLLTKKDLWKKVGGLDETNLKIAFNDID
                     YCLKIRSLGYDVVYTPYAKLYHYESKSRGVEDTPEKQKRFYGESNFMIEKWKTNRIPD
                     PFYNVNLTLKSEDFKIRK"
     gene            complement(747..1511)
                     /locus_tag="PKJ43_07815"
     CDS             complement(747..1511)
                     /locus_tag="PKJ43_07815"
                     /inference="COORDINATES: protein motif:HMM:NF015527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HNZ72512.1"
                     /translation="MINSDTDLRYNKLFFLHIPKTAGTSVNEMLANGFRSSNVRFHIE
                     NDRNEHYRNIDSGKYDMLSGHVRVDEMLHFFPGDKYLRVTLLRDPFEQLLSHINWVKY
                     ISENPQSSFFNGHPEFIKQFSLLLRGLDFDDESAVEKFFGNLPDIGISLFNNCQTRYL
                     FRGNPAPMLLETDALKAIESLNLFHVVGVVEQLDQFVREVFDKMEWKLKPKILQKNVQ
                     KHNYSIHDKNDRLKELLFPLYCADMVVYEYAKANIH"
     gene            complement(1532..>1714)
                     /locus_tag="PKJ43_07820"
     CDS             complement(1532..>1714)
                     /locus_tag="PKJ43_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ72513.1"
                     /translation="DQNFKKKADELFHNRFVKGKTIIHVSHQMDSIKKYCDRVMLLDK
                     GKVVKIGTPDEVLAEY"
BASE COUNT          564 a          332 c          308 g          510 t
ORIGIN      
        1 gtggaagacg ttgaagatgg aattcaaggt cttgaaaaaa taaaaaacag cgatcatcgc
       61 atcaaagtct tcgaatataa tgtgcccttc aatttctcag caattaacaa ttgggctata
      121 gaacaatgcg aaggcgaata catacttctg cttaataacg acataaaagc catctccgat
      181 ggatggctca acgcaatggt cgaacatatt cagcttgaaa acgttggcat agttggggct
      241 aaactcctgt atagcgacga caccattcaa cacgcaggag ttatcattgg tatacaaggc
      301 gttgcaggtc attcgcataa attttgtccc gatcacagtg tagggtattt ttatcgtccg
      361 gtggttattc aggaactatc ggcggtaaca ggtgcctgct tgcttaccaa aaaagatttg
      421 tggaaaaaag taggtggact tgacgaaaca aatctgaaaa ttgcctttaa cgatattgat
      481 tactgcctca aaataagatc attaggctat gatgtagtat atacacccta tgccaaacta
      541 taccattacg aatcgaaatc gcgcggagta gaagatacgc ccgagaaaca aaaacgcttt
      601 tatggcgaaa gcaattttat gattgaaaaa tggaaaacga acagaattcc cgatccgttc
      661 tacaatgtaa atctaacact caagtcggaa gattttaaaa taagaaaata aaatattctg
      721 aaaagtaaaa tgcgggaaga aattcttcaa tgaatatttg ccttagcata ttcataaaca
      781 accatatcag cacaataaag tgggaaaagt agctccttca atcgatcatt cttgtcgtga
      841 attgaatagt tatgtttttg gacattcttc tgcaaaattt ttggtttcaa cttccattcc
      901 attttatcga atacctcacg aacaaactgg tcgagttgct caacaacacc taccacatga
      961 aataaattca acgattcgat agccttcaga gcatctgttt cgagcaacat tggagctggg
     1021 tttcctctaa ataaatagcg agtttggcaa ttattgaata agcttattcc aatatctggg
     1081 agatttccaa agaatttctc tactgctgat tcatcgtcga aatcgagacc tcgtaacagc
     1141 aaagagaatt gtttaataaa ttcaggatgc ccattaaaaa aactgctttg aggattctcg
     1201 cttatatatt ttacccaatt aatatgtgaa agaagttgct caaatggatc acgtaacaat
     1261 gtaactcgta agtatttatc gccgggaaag aagtgcagca tctcgtctac cctcacatgt
     1321 cccgacagca tatcatattt cccagagtca atattccggt agtgctcatt tcgatcattt
     1381 tcgatatgaa atcttacgtt tgaagaacga aagccattgg caagcatctc gttaaccgac
     1441 gttcctgctg ttttcggaat atgtagaaaa aacaatttat tataacgcaa gtcagtgtct
     1501 gaatttatca tttttatatg agtacgtttt actaatattc ggccaacact tcatcgggcg
     1561 tgccaatttt cactactttc cccttatcga gcaacataac ccgatcgcaa tattttttaa
     1621 ttgaatccat ttgatgactc acatggataa ttgtttttcc cttaacaaaa cggttatgga
     1681 acaattcatc tgcctttttt ttgaaatttt gatc
//
LOCUS       DAOPUW010000471         1712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_77937_length_1712_cov_3.392275, whole genome shotgun sequence.
ACCESSION   DAOPUW010000471 DAOPUW010000000
VERSION     DAOPUW010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77937_length_1712_cov_3.392275"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PKJ43_07825"
     CDS             <1..180
                     /locus_tag="PKJ43_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008658384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNZ72514.1"
                     /translation="VRGYINKEIADKLNISKTTVISHRNNIAEKLGDRSIGRLTIYAV
                     LNGIVDLHELCLSEP"
     gene            complement(251..>1712)
                     /locus_tag="PKJ43_07830"
     CDS             complement(251..>1712)
                     /locus_tag="PKJ43_07830"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNZ72515.1"
                     /translation="VVLDSVYIEEVHENPLNVYNCQTTISNSRIHSDYEGCDGINIKR
                     GKILIDHCDFRGNNGLDSDALDFGEMPSGNAIVKNSYFHDFEGYNSDAIDLGDHAKNV
                     IIDSIIVYNIDDKGISIGQQSSAKISNSVFINCGMGAGMKDSSNVNIDHCTYYGNFYA
                     LANYQKHPGDAGSNLTVTNSVLSNSYELGYFNDEYSAISISNSLDDTEKLPSGNNNIH
                     ENPFFTNPTEYDFSLLPESPLIGAGTNGTTIGANLLLPALPVSVMISDIAYVTEQGTD
                     ILEFIGLYNPGNSPISLDSCSFTEGIAYTFPVGTSIEPKRKIYITSNAVSSFWEGKVA
                     VIHQWKSGRLADEGEKIQLSNNIGKVIDQVIYNNKAPWPLPSKPSEGITLTRFDVDNH
                     FGENWKLLSIDEMVSTQTHLINKSFSAYPNPTTNTITINGLEKSQGYEVINLQGKVIM
                     RGTADSETISIDLSGQENGIYLIRSGNVSRKILLMK"
BASE COUNT          464 a          338 c          361 g          549 t
ORIGIN      
        1 gtgcgagggt acattaataa agagattgca gacaagctca acatttctaa aacaacagtt
       61 atttctcacc ggaacaatat tgccgagaag ctaggcgacc gctctattgg ccgattaact
      121 atttatgcgg tgctgaatgg cattgtcgac cttcacgaac tttgcctgtc ggagccgtga
      181 tgattgcaaa ataaaactga gaactagaaa gtatcgttac attctgctaa aatgaatatc
      241 agggaatttt ctatttcatc aacaaaatct ttcgcgatac atttcctgaa cggatcaggt
      301 agatgccatt ttcttgtcct gaaagatcaa tgcttattgt ttctgaatct gctgttcctc
      361 tcataatgac ttttccctga agattaatta cttcgtagcc ttggctcttc tctaagccgt
      421 ttattgtgat agtgttcgtt gttggatttg ggtaagccga aaacgatttg tttatcaaat
      481 gcgtttgtgt actaaccatt tcatcaatag atagcaattt ccaattttcg ccaaaatggt
      541 tgtctacatc gaaacgggtc agggtaatgc cttcgcttgg tttcgagggc aggggccacg
      601 gagctttgtt gttgtatatc acctgatcga ttacctttcc gatgttgttc gacagttgta
      661 ttttttcccc ttcatcggca aggcggccac ttttccattg atgaataaca gcaacctttc
      721 cttcccaaaa tgacgaaaca gcatttgagg ttatgtatat ctttctttta ggttctattg
      781 acgttcccac cggaaacgta taggcaatgc cttcggtaaa tgaacaacta tcgaggctga
      841 tcggagagtt gccggggtta tagagcccaa taaattcaag aatatcagtg ccttgttcgg
      901 taacgtaggc aatatcgctt atcattacag aaacagggag cgccggaagt aagaggttgg
      961 caccaatagt tgttccgttg gttccggcac caataagcgg cgactctggc aacagtgaaa
     1021 aatcatattc tgttgggttt gtgaagaatg gattttcatg gatattatta ttccctgaag
     1081 gtaatttttc ggtgtcatcg agagaatttg aaatgctgat ggctgaatat tcatcgttga
     1141 agtagcctaa ttcataagag tttgaaagaa ctgagttggt tactgtcaag tttgaacctg
     1201 catcgcccgg gtgcttttga taattggcca gtgcataaaa gttcccgtag taagtacaat
     1261 gatcgatatt tacgttgctc gaatctttca ttccggcacc cattccgcag tttatgaaga
     1321 cagaattcga aatttttgcc gaagattgtt gtcctatcga aattccttta tcatcaatat
     1381 tatacacgat aatgctgtcg ataattacat ttttggcatg gtcgcccaaa tcaatcgcgt
     1441 cggagttata gccttcaaaa tcatgaaaat agctattttt tacaatggca tttcccgagg
     1501 gcatttctcc aaaatcgagt gcatctgaat caagaccgtt gtttcctctg aagtcgcaat
     1561 gatcgattaa aattttaccc cgttttatgt tgattccgtc gcagccttca taatcggaat
     1621 gaatgcggct gttgctgatt gttgtttggc aattatatac gttgagcgga ttttcatgaa
     1681 cctcttcaat gtaaacactg tcgagtacaa cg
//
LOCUS       DAOPUW010000472         1708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_78246_length_1708_cov_3.086509, whole genome shotgun sequence.
ACCESSION   DAOPUW010000472 DAOPUW010000000
VERSION     DAOPUW010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78246_length_1708_cov_3.086509"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..1708)
                     /gene="guaB"
                     /locus_tag="PKJ43_07835"
     CDS             complement(242..1708)
                     /gene="guaB"
                     /locus_tag="PKJ43_07835"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNZ72516.1"
                     /translation="MAERVQFEGLTFDDVLLIPSYSDVLPREVDLSTRFTRHITLKIP
                     IVSAAMDTVTESNMAIAIAREGGIGVIHKNMSIAEQARQVEIVKRAENGMISNPVTIL
                     KGATVGNALALMAEFKIGGIPVVDESGYLVGIVTNRDLRFQRDFYKPVEEVMTKENLI
                     TTSRSITLDEASEILQQYKIEKLPVVDDKNKLIGLLTYKDITKAKDKPMASKDKQGRL
                     RVAAAVGVAADTFERMDALVAAEVDAIVIDTAHGHSKGVIDMLKKARKAYPNLDVIVG
                     NIATAEAARDLAEAGADAVKVGIGPGSICTTRVVAGVGVPQLSAIFEVSKALKDLDVP
                     IIADGGIRYSGDIVKALAAGGETIMAGSLFAGVEESPGETIIFNGRKFKSYRGMGSLE
                     AMEKGSKDRYFQDMEDDIKKLVPEGISGRVPFKGSLYEVVYQLIGGLRAGMGYCGAHN
                     INELHQARFTRITNAGVVESHPHDVTIISEAPNYSRGE"
BASE COUNT          511 a          396 c          299 g          502 t
ORIGIN      
        1 accggatcat tggtctgtgc aaacaaacat gccgaaagcg acaatgctgc cgatagagaa
       61 agaagggata atttcataga taaaaaacga ttaatatttt tactcactaa tttgaaacat
      121 tgtgcttaaa aacttgcttt atcagctttt cgaaagcaaa attttcattg cactgcaaaa
      181 aaattaacga aacaaaaaag ataatgttgt aaaaatttga ttttttttga attttaagct
      241 tttattcgcc tctgctgtaa ttgggcgctt cgctgataat ggtaacatca tgaggatgac
      301 tttctactac acccgcattg gtaattctgg taaatctggc ctgatgcaat tcgttaatgt
      361 tatgcgctcc gcaataaccc atgcctgcgc gcaacccgcc aattaactga taaacaactt
      421 cgtataaaga acctttaaat ggtacacgac cggaaattcc ttccggaaca agctttttaa
      481 tatcgtcttc catatcctgg aaatagcggt ctttggaacc tttttccatc gcttcgagcg
      541 aacccattcc acgataggac ttaaatttcc gtccattaaa aataatggtt tctcccggtg
      601 attcttcaac tccggcaaaa agtgaacctg ccatgatcgt ctctcctccc gctgctaagg
      661 ctttaacaat gtcgccggaa taacgaatgc cgccatctgc aataatgggg acatccaaat
      721 ctttgagcgc tttagaaact tcaaaaattg ctgacaactg tggaacgcca acccctgcta
      781 ctacacgcgt tgtacaaata gaacctggac caataccaac tttaacggca tctgctccgg
      841 cttcggccaa atcgcgagct gcttcagctg ttgctatatt tccaactata acatcaagat
      901 tgggatatgc tttgcgtgct tttttcaaca tatcgataac cccttttgag tgcccatgag
      961 cagtatcaat aacaatagca tccacttctg cagcaaccaa tgcatccatg cgctcaaaag
     1021 tatcggctgc aactcccact gcagcagcaa ctcttaaacg tccctgtttg tccttcgagg
     1081 ccataggttt atctttggct ttcgtaatat ctttataagt caacagacca atcagcttgt
     1141 ttttgtcatc gacaacaggt aatttttcga ttttgtattg ttgcaaaatt tccgaagctt
     1201 catccaaagt aatggaacgg gaagtcgtaa tcagattttc cttcgtcata acttcttcca
     1261 caggtttata aaaatcgcgc tgaaaacgca aatcgcgatt ggtcactatt cccaccaaat
     1321 aacctgattc atctacaaca ggaataccac caattttaaa ttcggccatc aaagccagcg
     1381 cattgccaac agtagcacct tttaaaatag ttaccggatt ggaaatcatt ccattttctg
     1441 cgcgtttaac aatttcaacc tgtcgtgcct gctcagcaat ggacatattt ttgtggatca
     1501 ctcctattcc accttctctg gctatggcga tagccatatt tgattctgtt accgtatcca
     1561 tagctgccga aactatgggg attttcaaag tgatatggcg agtaaaacgt gtggataagt
     1621 caacttcacg aggtaaaaca tcggaatatg atggaataag caaaacatcg tcaaatgtta
     1681 aaccttcaaa ttgaactctt tctgcaat
//
LOCUS       DAOPUW010000473         1707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_78298_length_1707_cov_4.214286, whole genome shotgun sequence.
ACCESSION   DAOPUW010000473 DAOPUW010000000
VERSION     DAOPUW010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78298_length_1707_cov_4.214286"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1538)
                     /locus_tag="PKJ43_07840"
     CDS             complement(<1..1538)
                     /locus_tag="PKJ43_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72517.1"
                     /translation="MAALLIYLIKSTICLTLFYLVFRWTLKKERLFSLNRILLLAILV
                     LSATIPLVKTPTLFHQNIEVKAFPETNRVALPDFDDVAMQITTIPVNNNTLSTEKSIT
                     VEQVSILVYSVGFFLLLAALLIGLTKLMLLLFQTRIYRRKKYRLALVNRPIAPMSFGH
                     FIIISKHDYRENKNEILNHEMAHIQFRHTYDLLMVELVKLFHWFNPFVYQLRNDLKEI
                     QEFQVDRHLLNSGMDIKKYQLLLIKKCVGAERFALSNSFNHCQIKNRITMMNNQKKRK
                     SNSWKVAIFLPVAALMLMAFGKPGDLISSKIQTSDIKKNWTESDFGKPNYADHEYLGR
                     SIHINSNFEVVVNGKINDWDEVSKQIWECMDYNSTTTNLKKGFEKTVIDGKEVMVQNY
                     IELTVWKDISTPDDIYQNFLNFIGGEVLKIRQKYANEIYKNSYDQLTQNQKNSIDKVV
                     PAIISVQKMPAINSDFQKSDRPSLNIEVRAEGIYILPDKKVLSLQEMKEKVKEFADKP
                     DHWMV"
BASE COUNT          460 a          333 c          334 g          580 t
ORIGIN      
        1 accatccaat gatctggttt gtctgcgaat tcttttactt tttctttcat ctcttgcaag
       61 gaaagaactt tcttgtcagg taatatgtaa atgccttctg cccttacttc aatatttaat
      121 gaaggccgat cggatttctg aaaatcgcta tttatggcag gcattttttg aacagaaata
      181 atggctggaa ctaccttgtc gatagagttc ttttgattct gtgttaattg atcgtaactg
      241 ttcttgtata tttcattggc gtatttttga cgaattttta gaacttcgcc tccaatgaag
      301 ttcagaaagt tctggtatat atcatcggga gttgaaatat ctttccaaac agttaactca
      361 atataatttt gcaccataac ttccttgccg tcgattacgg tcttttcaaa tccttttttt
      421 aagttagtcg ttgtagaatt ataatccata cattcccaaa tctgttttga tacctcatcc
      481 cagtcattta ttttcccatt tacaacaacc tcgaaattag agtttatatg tattgatctt
      541 cccaaatatt catggtctgc gtaattcggt tttccaaaat cagattcagt ccagtttttc
      601 ttaatgtcag aagtttgtat cttgctggat atcaaatctc ctggttttcc aaatgccatc
      661 agcattagtg cagcaacggg caggaagatc gccaccttcc agctattgct ttttcgtttt
      721 ttttgattgt tcatcatagt aatacgattt ttaatttgac aatgattaaa actatttgaa
      781 agtgcgaaac gctctgcgcc cacacatttt tttatcaata gcaattggta tttctttatg
      841 tccatgcccg agttgagcaa gtgcctgtca acctgaaatt cctgaatttc ttttaggtcg
      901 tttcgcaact ggtaaacaaa ggggttgaac caatgaaaga gtttgacaag ctcaaccatc
      961 agcaagtcgt aagtgtgcct gaattggatg tgtgccattt catggttcag aatttcgttt
     1021 ttgttctctc ggtagtcgtg cttcgagata ataataaagt gaccgaaact catgggcgca
     1081 atggggcggt ttaccaacgc cagtctgtat ttttttcgtc gatatattct ggtttggaac
     1141 aacaaaagca tcagtttggt aaggccaata agcaacgcag cgagaagcaa gaagaagcca
     1201 acggaataaa caaggattga cacctgttca accgtgatgc ttttttctgt agataaagtg
     1261 ttgttattca ccggaatggt ggtaatttgc atggccacgt cgtcgaaatc gggcaaggct
     1321 accctgtttg tttcaggaaa agcttttact tcgatgtttt gatgaaagag tgtgggcgtt
     1381 tttaccaacg gaatggttgc cgatagcacc aatatggcca aaagcagtat gcggttcagc
     1441 gaaaaaagcc tctccttttt taacgtccat ctgaaaacca agtagaacaa ggtaaggcag
     1501 atggtcgatt tgatgaggta aatgagcagg gcagccattt tattccgttt ttgagtctcc
     1561 tttttcaact tgcctaatca gctcgcgtat ttcttcggtc gatatttcct gttcttcaac
     1621 aaacatggag accacactgg agtaagagcg gttaaagtac tttgccacaa agtctttcat
     1681 ggcattcttg ccatattcat ctttcga
//
LOCUS       DAOPUW010000474         1701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_78725_length_1701_cov_3.393074, whole genome shotgun sequence.
ACCESSION   DAOPUW010000474 DAOPUW010000000
VERSION     DAOPUW010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78725_length_1701_cov_3.393074"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..657)
                     /locus_tag="PKJ43_07845"
     CDS             complement(136..657)
                     /locus_tag="PKJ43_07845"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNZ72518.1"
                     /translation="MTTLTRKLQNSFISMIDWFYSPFSRFLSEETFRYGVTGGANTML
                     DIFLYFVFYNFIIDKQIVDLGFIAISPHIAAFLFVFPITFSTGFLLAKYITFTQSEIR
                     GRVQLFRYSISVAGSILLNYVLLKFFVEVAGLWATLSKMLTTFVVVIYSFIIQKYFTF
                     KTGKKVFSGSRDY"
     gene            complement(753..>1701)
                     /gene="dacB"
                     /locus_tag="PKJ43_07850"
     CDS             complement(753..>1701)
                     /gene="dacB"
                     /locus_tag="PKJ43_07850"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNZ72519.1"
                     /translation="DVGNHFGAAATGLAIFDNVFCMEFNVPDTPGQPAILAKVFPDIP
                     NLSIDNKVFSSSINSDQAYVFGSTFNSDRLVKGTLPAGAKGFVVKASMPDPAVVLANQ
                     LLRVLTDSSVVFLGKIEQRKVAYIQQIEKSKVLIRHLSPALSAIIDMMNKESDNLFAE
                     TLLKHLGLKLLGDGSTLAGCKAITSYWNSKEIGKNALFVSDGSGLSRLDAFSSGTLAN
                     LLVYMKKTSAWSDTFEQSIPLAGVEGTQKSYFQQSPVKERIRAKTGSMTRVRSMAGYM
                     LTQRGHNVAFSVLLNNYSGSGSTVKKEIEKIVELIYSEL"
BASE COUNT          564 a          347 c          319 g          471 t
ORIGIN      
        1 ggttatgatt ttgcgttagt gcacaaaata aacaattgtt aaatagtagg tttatttaat
       61 taattgactg ggttattatt agatttgaaa agccgcctga aaaggtggct ttttttatgt
      121 tgtaaaactg ctatatcaat agtcgcgtga gccggaaaat acttttttcc cagtcttgaa
      181 cgtgaaatat ttctggatga taaaactgta aattacaaca acaaacgttg ttagcatctt
      241 cgatagagta gcccataagc cggcaacttc aacaaaaaac ttcagtaaaa catagttgag
      301 cagaattgaa cctgctacag aaatagaata acgaaacaac tgaacccgcc cacgtatttc
      361 cgattgggta aaagtgatat acttggccaa taaaaacccg gtagaaaaag taataggaaa
      421 aacaaacaga aaagcagcaa tgtgcggact aattgcaata aagccaagat caacaatttg
      481 tttatcgata atgaagttgt agaatacaaa atacagaaaa atatcgagca tggtattggc
      541 tccaccagtt acaccatacc tgaaggtttc ttcagataaa aaacgactga aaggagaata
      601 aaaccaatca atcatgctga taaaactatt ctgcagcttt cgggttaagg ttgtcatcaa
      661 ttttaaattg aacaaaaagc gaagataatt ctttttttat ttctcttgca aaaatataaa
      721 agcccggaaa aggtcaccaa cacatgcaaa aactataatt cgctgtatat taattcaact
      781 attttttcaa tttctttttt aactgtagat ccactacctg aataattgtt caacaaaact
      841 gaaaaagcca cattgtggcc tcgctgggtt agcatgtacc cggccatgct tcttacccgg
      901 gtcatggagc ccgtttttgc gcgaattctt tctttgaccg gtgattgctg aaaatacgat
      961 ttttgtgttc cttcaactcc cgccaacggg atcgactgtt caaaagtatc gctccaagca
     1021 gatgttttct tcatgtaaac caacaaattg gcaagcgtac cagaagagaa tgcatcaagc
     1081 cgcgacaagc cacttccatc ggaaacaaaa agggcatttt ttccgatctc cttcgaattc
     1141 caatatgaag ttatagcctt acagcccgct aaagtagagc catcgcccag caacttaagg
     1201 ccaagatgct tgagtagcgt ttctgcaaac aggttatcgc tttctttatt catcatgtcg
     1261 ataattgcag ataaggctgg tgacaagtgc ctgataagca ctttgctttt ctcaatttgc
     1321 tgaatatagg caacttttcg ttgttcaatt tttccaagaa aaacgaccga cgagtcggta
     1381 agcacgcgaa gcaattggtt agccaataca acagccggat cgggcatcga agctttcaca
     1441 acaaacccct tggctccagc aggtaaggta cctttcacca agcggtcgct attgaatgta
     1501 ctcccaaaga catacgcctg atcgctatta atactagatg aaaaaacctt attatcaatg
     1561 gataaatttg gaatatcggg gaaaactttt gccaatattg cgggctgccc cggagtatcg
     1621 ggcacattaa attccatgca gaaaacattg tcgaaaatgg caagtcctgt agccgcagct
     1681 ccaaaatggt ttcctacatc t
//
LOCUS       DAOPUW010000475         1699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_78897_length_1699_cov_2.380170, whole genome shotgun sequence.
ACCESSION   DAOPUW010000475 DAOPUW010000000
VERSION     DAOPUW010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78897_length_1699_cov_2.380170"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKJ43_07855"
     CDS             complement(<1..222)
                     /locus_tag="PKJ43_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72520.1"
                     /translation="MKKFLTYRILPAIAILLFALNSRGQAYLQDPKFGDSQEARQECA
                     ANLSLYQEFYNQRDFNRAKPLWMKVLKNCP"
     gene            complement(250..1416)
                     /locus_tag="PKJ43_07860"
     CDS             complement(250..1416)
                     /locus_tag="PKJ43_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72521.1"
                     /translation="MRVRGYANTKAMGGLSQGIRNEQWINYLNPASYTAQDTMSFVFD
                     FGVEGDGLGSKSGQQSNYNTTANIHHLAIQFPVTRWLGASAGIQPFSKVGYRIRHVET
                     NPEILSQLGAIKYYHIGNGGITQFYLGTAIEPLKNLSFGINMSYLFGGLEHNSEVVFP
                     ENSNCINIRKINSVVARDIAFSFGVQHASFFGAEKQFKLVVGATLDNETAIGARRIEL
                     IQYSSGNSNDTIFYKEYPKGSLTFPRNLTAGFTFSYMSNFMLGFEYGYQDWTNARFLN
                     AADSLTKSQSFSGGIQITPNPNDLKYYYKRISYRAGYHYTNTNIKLKGNQINDYGISF
                     GLGLPFRRTNTSFNCSVEVGRKGTLKDNLVRETYGILNIGFTFYDFWFIKRKFN"
     gene            complement(1517..>1699)
                     /locus_tag="PKJ43_07865"
     CDS             complement(1517..>1699)
                     /locus_tag="PKJ43_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNZ72522.1"
                     /translation="IINEVDGYIDSIRRAYSDVKVVFTGGESFFFDKKLKNTIFVDSN
                     LLLFGLNRILTHNVSQ"
BASE COUNT          478 a          359 c          345 g          517 t
ORIGIN      
        1 ggggcagttt ttcagtactt tcatccaaag gggtttagcc ctgttgaaat cccgctgatt
       61 gtaaaactcc tggtataacg atagattggc cgcacattct tgcctcgctt cctgagaatc
      121 accaaatttt gggtcctgta ggtatgcctg acccctgctg ttcaatgcga acaacagaat
      181 tgctattgcg ggtaaaatcc tgtatgtcaa aaactttttc atggtaaaca gtgtgtttta
      241 tgaatattac taattgaatt ttctcttaat aaaccaaaaa tcgtaaaaag taaaaccgat
      301 gtttagaatc ccgtacgttt ccctaactaa attatccttt aaagttcctt tccttccaac
      361 ttcaaccgag cagttaaagg atgtgttggt tctacggaac ggtaatccta atccaaaact
      421 tatgccataa tcattgatct gattcccttt gagttttatg ttggtattgg tatagtgata
      481 accagcccgg tagctgatac gcttatagta gtattttaaa tcgttggggt tgggggtaat
      541 ctgtatgcct ccactgaagg attggctttt ggttaaggaa tccgcagcat tgagaaacct
      601 tgcgttcgtc caatcctggt aaccatattc aaatccaagc ataaagttgc tcatgtacga
      661 aaatgtaaag ccagcggtaa gatttctggg aaatgttaac gaccccttgg ggtattcctt
      721 gtagaaaatg gtgtcgtttg aattgccgct ggaatactga atcaactcaa ttctccttgc
      781 tcctatggcc gtttcattgt caagagtggc accaacaacc agtttgaact gtttctccgc
      841 cccgaaaaaa cttgcatgtt gcacaccaaa gctgaaagca atgtctcggg ctacaacgct
      901 attgatttta cgaatattaa tacaattgct attctcaggg aaaacaactt ccgagttatg
      961 ctcaaggcct ccgaaaaggt aggacatatt tatcccaaac gatagatttt taaggggctc
     1021 tatggccgtt ccaagataaa actgcgtaat ccctccgttc ccgatatggt agtattttat
     1081 tgcgcccagt tggctaagaa tttcgggatt tgtttcaaca tgcctgatgc ggtaccccac
     1141 tttgctgaat ggctgaatgc ctgcgcttgc gcctaaccag cgggttaccg gaaattgtat
     1201 tgccaaatgg tgtatgttag cggtggtgtt atagttgctc tgctgacccg atttacttcc
     1261 taaaccatca ccttcaacgc caaaatcgaa aacaaacgac atggtatcct gagcagtgta
     1321 cgaggcgggg ttcaggtagt taatccactg ttcgttgcga attccctgcg atagtccacc
     1381 cattgccttg gtgttggcat atccacgtac tctcatctcg ccaatgccaa attttgaata
     1441 gggcgaatag gttacgaaac tttgcgaatt ggctgagatg aatgagacaa gtagtaaaac
     1501 tgcaattgca actaatttac tgcgatacat tatgcgttag aattcgattt aatccaaaaa
     1561 gcaaaagatt tgaatctaca aagatggtat tttttaattt tttatcaaaa aaaaatgatt
     1621 cgccgccggt aaaaacaact ttaacatcgc tgtatgccct cctgattgag tcgatatagc
     1681 catcaacctc gtttattat
//
LOCUS       DAOPUW010000476         1695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_79154_length_1695_cov_3.378659, whole genome shotgun sequence.
ACCESSION   DAOPUW010000476 DAOPUW010000000
VERSION     DAOPUW010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79154_length_1695_cov_3.378659"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PKJ43_07870"
     CDS             <1..825
                     /locus_tag="PKJ43_07870"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNZ72523.1"
                     /translation="DIRKVVQGKNPKLAGKVPGFIYNWLTRILHLKELNAFMLQYGHL
                     KGVEFVNEGIKFLNINYELIGTENIPKEGKFIFVGNHPLGGLDGMIVLKYVTEALGKT
                     RSISNDFLLEITPLKEWFVPINKVGSQSRNYANLVEDLYKSDDQILIFPAGLCSRKIN
                     GKIVDLTWQKHFIQKAVEHKIDIIPFFFDGRNSNFFYNLAKIRKFFRIKVNIEMMYLA
                     DEMFKHKNKTFRIYFGKPIPYSTFNQTKRPIEWAEEVKKITYQLPKQKAMGNNQFI"
     gene            851..>1695
                     /locus_tag="PKJ43_07875"
     CDS             851..>1695
                     /locus_tag="PKJ43_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ72524.1"
                     /translation="MKDIIPKIPSDLIEAELTNDKFLRDTNKGGNKIYVLTASDSPNI
                     MQEIGRLREITFRAAGGGTGLETDIDIYDTGEMPYKQLIVWDPDAKEILGGYRYILCD
                     KLIYNSNGDPNLATAHLYHFSEEFKTKFFPYTIELGRSFVQPDYQSSKAGAKALYALD
                     NLWDGLGALIVDHPEMKYFFGKFSMYTHFNIEARNYILSYLKKYFNDDDNLVVPKEKY
                     LITIDDSLYNPIFTENDLKEDYKILSKKVRELGENIPPLVNAYINLSPTMRSFGTTLN
                     DEFGDV"
BASE COUNT          575 a          343 c          312 g          465 t
ORIGIN      
        1 gacatcagga aagtagtgca agggaaaaat cctaagctag cggggaaagt tcctggtttt
       61 atctataatt ggcttacaag aatattgcat ctaaaagagc tgaatgcatt catgctgcaa
      121 tatggacatc ttaaaggtgt tgaatttgtc aatgaaggaa taaagtttct gaacatcaat
      181 tatgaattga tcggcaccga gaatatcccc aaagagggaa aatttatttt cgttggaaac
      241 cacccgcttg gcggtctcga cggaatgatt gtgctgaagt atgttaccga agcattggga
      301 aaaacccgtt ctatctctaa cgatttcctt ctcgaaatta ccccgttaaa agagtggttt
      361 gtccctatca ataaagtagg ctcacaatca cgcaattacg ccaatttagt tgaagatctt
      421 tataaatccg atgatcagat attgattttt ccggccgggc tatgttcgcg caaaattaac
      481 ggaaaaattg tagacctcac ctggcaaaag catttcatac agaaagctgt cgaacacaaa
      541 attgacatta ttcctttctt cttcgacggc agaaattcca actttttcta caatcttgcc
      601 aaaatccgca aattcttcag gattaaagtg aacatcgaaa tgatgtattt agcagacgag
      661 atgttcaaac ataaaaataa aacattcagg atttattttg gaaaacctat cccatactca
      721 acattcaacc aaacaaaaag gcctatcgaa tgggccgaag aggttaagaa aataacctat
      781 caattgccca aacaaaaagc aatgggcaat aatcagttta tttaatcatt gaagttgaaa
      841 cttttacatt atgaaggata taattccaaa aattccgtca gatttaatcg aagcggaact
      901 tacaaacgat aaatttctca gagatactaa taaaggagga aacaaaatct atgtgttaac
      961 ggcaagcgat tcgccaaata tcatgcagga aattgggcgc ttacgcgaaa taaccttccg
     1021 tgctgcgggt ggcggtacag gcctcgaaac cgacatagac atttacgaca ccggcgaaat
     1081 gccatacaaa cagctcattg tgtgggaccc cgatgcaaag gaaattttag gtggataccg
     1141 ctacatatta tgcgataaat tgatttacaa cagcaatggc gaccccaact tagctactgc
     1201 tcacttgtat catttctcag aagagttcaa aaccaaattt ttcccttata ccatcgagtt
     1261 aggccgttca tttgttcagc ccgattacca gtcgagtaaa gcaggagcca aggctcttta
     1321 tgccctcgat aatctttggg atggattggg cgctttaatt gtcgatcacc ccgaaatgaa
     1381 atattttttc ggaaaatttt ccatgtacac tcatttcaac attgaagcaa gaaattacat
     1441 cttatcttat cttaagaaat acttcaacga cgatgacaat ttggttgtgc ccaaagaaaa
     1501 atatctcatc acaatcgacg actcactata caacccaata tttactgaaa acgacctgaa
     1561 agaagattat aaaatccttt cgaaaaaagt acgcgaattg ggagaaaaca ttcctccttt
     1621 ggtaaatgct tatatcaact tatcgcctac tatgcgctcc tttgggacaa cacttaacga
     1681 cgaatttggc gatgt
//
LOCUS       DAOPUW010000477         1694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_79220_length_1694_cov_3.861501, whole genome shotgun sequence.
ACCESSION   DAOPUW010000477 DAOPUW010000000
VERSION     DAOPUW010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79220_length_1694_cov_3.861501"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..>1694
                     /locus_tag="PKJ43_07880"
     CDS             326..>1694
                     /locus_tag="PKJ43_07880"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNZ72525.1"
                     /translation="MTPHVIYSIYGDHQGVTEIRQLLSSDKPATIQLKGVVGSAAAFI
                     IAALFDKINRPILFCLNDREESAYFYDDLSLMLSEENVVYFPSSYKRAARYGKPDHES
                     IIKRTEALNRISENQRCVVVTYPDALAEKVISREALISNTLKISVGDKLSPEFISDVL
                     AEYGFERVDFVYEPGQYSVRGGITDIFSYASDEPFRIDFFGNEVESIRNFSIDTQLSS
                     QRLSSIAIIPNIQQSIKEEKRIEITNYIPTDYVLVFKDSEYARLEIDKIYNETESFFL
                     GSQSSENAEEVPSPELLIDGTTFFAQSKAFHCIELGTSHHPKEFHVVQFSTVPQPRFN
                     KNFDLLAQELKLNWSNGYQNFILSNNEKQIERLQAIFDDKALNVRFSPLNFTINEGFI
                     DRELKMAFYTDHQLFERYHRYKMQTKQQARESITIKELNKLHQGDYVVHIDHGIGKFA
                     GLVKTE"
BASE COUNT          540 a          351 c          308 g          495 t
ORIGIN      
        1 tcttattttt aaccaaatta cttgaaaaat tacatcaata aagacatgac aagccatacg
       61 ctaaacaggc tattatctat actttagaaa tagtgtaccc ttgatttcaa catgtcaaaa
      121 aatacaactc ctcatttcga tgtaaaatcg atgttaaaat acaatgattt tttttaagaa
      181 gtaaagtcat ggctcagaaa tcataaatta tttgcaaaca aaaaatcagc gttcattcat
      241 tttacaaaac ggtcgaatat agctttctgt ttttgcgctc taagctggga tttggacttt
      301 agcttaatta tatttgcgcc ctattatgac accacacgtt atttactcta tatatggcga
      361 tcatcaggga gttaccgaaa tcaggcaatt gctttcttcc gataaaccag ccaccattca
      421 actcaaagga gttgttggtt cggctgcggc attcatcatt gctgctcttt ttgataaaat
      481 aaaccggcct atcctttttt gtttgaacga tcgcgaagag tcggcctact tttacgacga
      541 cctttcacta atgctctccg aagaaaatgt tgtgtatttt ccttcgtcgt ataaacgtgc
      601 tgcacgatac ggaaagccag atcacgagag tattattaag cggactgaag cacttaatag
      661 aatttcagaa aaccagcgat gtgttgtggt tacatacccc gatgccctcg ctgaaaaagt
      721 tatttcaaga gaagcattga tttcaaacac gttgaaaata tcggttggag ataaactttc
      781 gccagagttt attagcgatg tgcttgccga atatggtttc gaacgggttg attttgttta
      841 cgaacccgga caatattctg tccgcggtgg cattaccgat atcttttcct acgcgtcaga
      901 cgagcctttc aggattgatt tcttcggaaa cgaagtggaa tcgatccgca acttttcaat
      961 cgatactcag ctttcatccc aacgactttc gtcgatagcc attattccca acattcagca
     1021 gtcgataaaa gaagaaaaaa ggatagaaat aacaaattac attccaacag actatgtatt
     1081 agtatttaaa gattcagaat atgcccgatt ggagatcgac aaaatataca atgaaaccga
     1141 aagtttcttt ttgggctccc aaagcagtga aaatgccgaa gaagttcctt cgcctgaatt
     1201 gctgattgat ggaacaacat tttttgccca atcaaaagct tttcattgca tcgaattggg
     1261 aactagtcat caccccaaag aattccatgt cgtgcagttc agtacagttc cccaaccccg
     1321 gttcaacaaa aatttcgatt tgttagcaca agagctaaaa cttaactggt caaacggata
     1381 ccagaatttc attttatcga acaacgaaaa acaaattgaa cgtttacaag ccatttttga
     1441 cgacaaggct ttaaatgttc ggttttcccc tctgaatttt accatcaatg aagggtttat
     1501 cgatcgcgaa ctgaaaatgg ctttttatac cgaccaccaa ttgtttgagc ggtaccaccg
     1561 gtataaaatg caaaccaagc agcaagcccg cgagtcgatt acaattaaag aactgaacaa
     1621 actacaccaa ggcgattatg tggtgcacat cgatcatggc attggaaaat ttgccggcct
     1681 tgtaaaaacc gagg
//
LOCUS       DAOPUW010000478         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_79689_length_1688_cov_4.390692, whole genome shotgun sequence.
ACCESSION   DAOPUW010000478 DAOPUW010000000
VERSION     DAOPUW010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79689_length_1688_cov_4.390692"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..551)
                     /locus_tag="PKJ43_07885"
     CDS             complement(126..551)
                     /locus_tag="PKJ43_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNZ72526.1"
                     /translation="MLQKEQKEQIVNAIKEAECNTSGEVKVHIESHCPGDVMDRAAYW
                     FATLNLHKTEKRNGVLFYLAMKDRKFAILGDMGINAVVPENFWNEVKELMLPFFGEGN
                     YSEGLSKGIIMTGQKLKEFFLYQQENDVNELDDEISFGK"
     gene            complement(567..1160)
                     /locus_tag="PKJ43_07890"
     CDS             complement(567..1160)
                     /locus_tag="PKJ43_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNZ72527.1"
                     /translation="MKKGLISLLVIVLLVVIVGGSMIGSYNKIVKMEEEVSAAWSQVE
                     NVYQRRADLIPNLVNTVKGYASHERETLEAVINARAKATQTTIDPTKLDAASLQQFQA
                     NQSELSSALGRLMVVMEQYPDLKANQNFRDLQVQLEGTENRIAVERRAFNDTAKMFNS
                     YIRQFPKNIIAAIFNFEKKAYFEAEQGAEKAPEVSFE"
     gene            complement(1419..>1688)
                     /locus_tag="PKJ43_07895"
     CDS             complement(1419..>1688)
                     /locus_tag="PKJ43_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72528.1"
                     /translation="HNQYVSYWVKFGLLGLLFIIFLLIFVAVKKNLFRNGLFVLFLVL
                     QLVANMGDANWETHVGLAFFVFFFSLFLWHTNKTNAKDQESGALG"
BASE COUNT          503 a          362 c          288 g          535 t
ORIGIN      
        1 aaaaatcagt tttttcattc ttatttttct atatcattta atagttcgcc gacacgtttc
       61 ggcaacgatt ttcagcaaaa tcacactcaa caataaacat gtttttcttt tagattaact
      121 aatagttatt tcccaaaaga tatttcatcg tcaagttcat tgacgtcgtt ttcttgttga
      181 taaagaaaaa attctttcag cttttggcct gtcattatga tccctttaga gagtccttcc
      241 gaataattgc cttcaccgaa aaagggcaac attagctctt taacttcgtt ccagaaattt
      301 tcgggaacaa cagcgttgat tcccatatcg cccaaaatgg caaacttgcg atctttcatt
      361 gccaggtaga aaagcacacc attccgcttt tcagttttgt gcaaattaag agttgcgaac
      421 cagtatgcag cacgatccat aacatcgcca gggcaatggc tttcgatgtg cactttaacc
      481 tcgcccgacg tgttacactc tgcttctttt attgcattaa caatttgttc tttctgttcc
      541 ttctgtaaca ttttgcttca aattgtttat tcaaaactaa cttcaggggc cttttctgca
      601 ccttgttctg cttcgaaata ggctttcttt tcgaagttga aaattgctgc aataatattc
      661 tttgggaact gacggatata tgagttgaac atttttgcag tgtcgttaaa tgcgcggcgt
      721 tcaacagcaa tcctgttttc agttccttca agctgcacct gcaaatctct aaagttctga
      781 ttagctttca gatcgggata ttgttccata accaccatta aacgtccaag tgctgatgac
      841 aattccgact ggtttgcctg aaactgttgc aacgaggcgg catcaagctt agttggatcg
      901 attgtagttt gcgtagcctt tgccctggca ttaatcactg cttcgagggt ttcgcgttcg
      961 tgcgaagcat agccttttac tgtattcacc aaatttggga tgaggtcggc tcggcgctga
     1021 tatacatttt caacttgcga ccaggccgct gaaacttctt cttccatctt tacaattttg
     1081 ttgtaagagc caatcatgct acctccaaca attactacta gaagaacaat taccaataaa
     1141 gaaattaaac cttttttcat tcctatcatt tattttagtt cacggatgca gcctcgagat
     1201 ctagacttcg catgatggct ttttcatccg cagttgttat ttaacgaatc atgctcgttt
     1261 gaaatatttt taaattaaat caaaaatcat aaaaatgtaa cgaataacaa aacgtattca
     1321 acgggagaca aaaatatatg acattgtgga gctgcttgtg caaatatcgg cgaacttaat
     1381 tatttcaccg acaagaaacc ttttccggta gcagaatctt aaccaagtgc gcccgattcc
     1441 tgatcttttg cattagtctt attcgtgtgc caaagaaaaa gagagaagaa aaaaacgaag
     1501 aatgccaacc ctacatgagt ctcccaattg gcatcgccca tattagccac cagttgcaag
     1561 acaagaaaca agacaaaaag cccatttcgg aacaagttct ttttaacagc aacaaaaatt
     1621 agtaagaaga taataaagag taatccaagc aacccaaatt tcacccaata gctaacatat
     1681 tgattatg
//
LOCUS       DAOPUW010000479         1682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_80142_length_1682_cov_3.727105, whole genome shotgun sequence.
ACCESSION   DAOPUW010000479 DAOPUW010000000
VERSION     DAOPUW010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80142_length_1682_cov_3.727105"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1682
                     /locus_tag="PKJ43_07900"
     CDS             <1..>1682
                     /locus_tag="PKJ43_07900"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNZ72529.1"
                     /translation="QFIWFETYDGYYHCFNPQDESFSTLPKYMINLEEKYSKITCFFQ
                     YSDDEIWLGSSNSGVYRLLFDPAKESYSQQQFLSRGQYSISNNDIRFIVADVDRNLYI
                     GTKNGLNVLKRGDLVQGNFFFQHLYTGNSFTSAVSLGNDVWLGTQNSGLIYYSTSSRS
                     FFTYSTTNSPLESNRIDLLRTSNEGKILVGGNSLYIIESRDKPWKKVPFDGSHVDKIF
                     EDYKGLLWVTSGKYGVLQIDQSTGKSKHFDLTPKNYKYLSDKERPYFFEDSRKTLWIC
                     VHGGVLAQYQRESESFRFFRNDPRDPKSISSNTVMCIAEDKSGNLWVGTSLQGGVNKI
                     IFKNPAFKSVQTNETFDDNMENVIRSVMEDRNGNIWVASKGGKITIFDTAMQAISPGL
                     QYPFPSLGGLVYNVYSMFQDSRGYIWLGSKGAGLAVSTRPVNNISLGFNTIDFYRYSN
                     IENDTTSLCNDDIYCIDEDKSGRIWIGTYGGGLCCTKPVDYSTLTFGTVNSQTSNLSN
                     DMIRDILVDTENTLWVATTFGLNRASNISSQKDKFNFQVYYHDPQNPKSIAY"
BASE COUNT          518 a          302 c          366 g          496 t
ORIGIN      
        1 agcaatttat ctggttcgaa acttacgatg ggtattatca ttgtttcaat ccacaggatg
       61 aatcgttttc aaccttgcca aaatacatga tcaatctgga agagaagtac agcaagataa
      121 cttgtttttt tcagtacagc gatgatgaaa tttggctggg gtcctcaaat tccggggttt
      181 ataggctttt gttcgatcct gcgaaagaat catattcgca gcaacaattt ttaagtcgcg
      241 gacaatattc aatatcgaac aacgatatac ggtttattgt cgccgatgtt gaccggaact
      301 tatacattgg aactaaaaac ggactaaatg tccttaagcg tggagatttg gttcagggga
      361 acttcttttt tcagcatctc tatactggca acagctttac atcggcagtg tcgttaggta
      421 acgatgtttg gttgggtacc caaaattcgg ggcttattta ttatagtacc tcatcgcgtt
      481 ctttttttac atatagcaca acaaattcgc cactcgaaag taaccggatt gatttgttaa
      541 gaacaagcaa tgaaggaaaa atattggttg gcggaaatag tttgtatatc atcgaatcgc
      601 gcgataaacc atggaaaaaa gttcctttcg atggctcgca tgttgataaa atatttgaag
      661 attataaagg ccttctttgg gtaacgtcgg ggaaatacgg agttcttcag attgaccaaa
      721 gcacaggcaa aagcaagcat ttcgatttga caccgaagaa ctacaaatat ctttccgaca
      781 aagaaaggcc atatttcttc gaagattcaa gaaagacact gtggatttgt gttcatgggg
      841 gcgtactggc tcaatatcaa agagaaagtg aaagctttcg ttttttcagg aatgaccctc
      901 gcgatccaaa atcaataagt tcgaacacgg taatgtgcat agccgaagat aaaagtggga
      961 acctttgggt gggtacttcg cttcaggggg gcgtcaataa gattatcttt aaaaatccgg
     1021 cttttaaatc ggtacaaacc aacgaaacat tcgatgataa tatggagaat gtaatccgtt
     1081 cggtaatgga agatcgaaac gggaatatat gggttgcctc gaaaggtgga aaaatcacaa
     1141 ttttcgatac agcgatgcaa gcgataagcc cgggattaca ataccccttt ccatctttgg
     1201 gcggattagt atataatgta tattcaatgt ttcaggattc gcgagggtat atctggctgg
     1261 gttcaaaagg tgctgggttg gctgtttcta cgcgtccggt taacaatatt tcattgggtt
     1321 ttaataccat tgatttttat cgttattcaa acatagagaa tgacacaact tctttgtgca
     1381 acgatgatat atattgtata gatgaagaca agagtggacg gatttggatt ggtacctatg
     1441 gaggagggtt gtgttgtaca aaaccagttg attactccac gcttactttc ggaacagtaa
     1501 attcccaaac atcgaacttg tcaaacgata tgatcaggga tattcttgtt gacactgaga
     1561 atactctttg ggtcgccacg acattcggat taaaccgcgc ttctaatatc tcatcccaaa
     1621 aagataaatt caactttcag gtgtattatc acgatcccca aaatccgaaa agtattgcct
     1681 at
//
LOCUS       DAOPUW010000480         1681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_80241_length_1681_cov_2.850554, whole genome shotgun sequence.
ACCESSION   DAOPUW010000480 DAOPUW010000000
VERSION     DAOPUW010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80241_length_1681_cov_2.850554"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1681)
                     /locus_tag="PKJ43_07905"
     CDS             complement(<1..>1681)
                     /locus_tag="PKJ43_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNZ72530.1"
                     /translation="ITNSSGQPGDFVSIRIRGVGTNGDNEPLYIVDGLPTNGYGIDYL
                     NSTDIESVEVLKDAASAAIYGARGGNGVVLITTKKGKKGEKFNVSYDGYYGYQNPWHT
                     VDVLNAAEYMDAMNEAAANSNVKPDKLPFTDARRDTMRWDTDWQEEMLNRNAMKQNHV
                     ITMTGGSENSTYASSISYFKQDGIVAKGKSGFERLTYRLNTTRQFGMMTFGSNINLVQ
                     IDTKSVGANDMYDGSALSQALNLPPVVPVMFDNGKWATPTDFGLGMQEITNPIAMLSY
                     RNSGTKTRKAVANVWADFDFGYLIAPLSGLKFKTSYNYEYANTHYRSYSPIYDLDPTH
                     KSTVNGVSTTDNHYVSWNIDNTLTYVKSLGDHNFTLLAGHSAFRSYHESLHGSKSDVI
                     FDDFEHAYLDNATDPLSAITGGTYDEHTILSYFGRIDYDYANKYMLTATLRRDGSSRF
                     GTAKKYGYFPSLSAGWVISRESFFPETDAFNFLKIRASWGQNGNENIGDFQYISTMNS
                     NLIYYFGLNQDQVNGMQPAKIANPSLKWETSEQANVAFDFGFFNNKLTATVD"
BASE COUNT          431 a          344 c          384 g          522 t
ORIGIN      
        1 atcaacagta gcagtaagtt tgttattgaa aaacccgaaa tcaaaagcaa cgttagcttg
       61 ttccgaagtt tcccatttca aacttggatt ggcaatttta gcgggttgca taccatttac
      121 ctgatcctgg tttagaccaa aataatagat aagatttgaa ttcattgttg aaatgtattg
      181 gaaatcacca atgttttcgt tcccgttttg tccccaactt gcacgtattt tcaagaaatt
      241 aaaagcatcg gtttctggga aaaagctttc gcgcgaaatg acccaacctg ctgaaagcga
      301 aggaaagtat ccatattttt tagcagtccc gaagcgtgaa gaaccgtcgc gtcgcaacgt
      361 ggcagtgagc atatacttgt tagcatagtc gtaatctatt cttccgaaat acgataagat
      421 cgtatgctcg tcgtaggttc caccggttat ggcgctaagc gggtcggtgg cgttgtcgag
      481 atatgcatgt tcaaaatcgt cgaaaatgac atcgcttttc gatccgtgca aactttcgtg
      541 gtacgagcgg aaagctgagt ggccggcaag caatgtaaaa ttatgatctc ccaaagattt
      601 tacataggtc agtgtgttat cgatgttcca gcttacgtaa tggttatcag tagtgctaac
      661 cccgtttact gtgcttttat gggttgggtc gaggtcgtaa ataggcgagt agcttctgta
      721 atgtgtgttg gcatactcgt agttatacga agttttgaat ttcagtcctg aaagtggagc
      781 aatgaggtat ccaaaatcaa agtcggccca aacattggct acagctttgc gtgtttttgt
      841 tcccgagtta cggtagctta gcatggctat agggttggtt atttcttgca ttccaagccc
      901 gaaatctgtt ggtgtggccc attttccatt gtcgaacata accggaacaa caggaggaag
      961 gttcaaggct tgtgatagtg cacttccgtc gtacatgtcg ttggctccta ccgatttggt
     1021 gtcgatctga acaaggttga tgtttgaacc gaaagtcatc attccaaatt gtcgggtggt
     1081 attgaggcga taagtcaatc gttcgaaacc agatttccct ttggcaacaa tcccgtcttg
     1141 tttgaagtac gaaattgatg aagcataagt agaattttct gaaccaccgg tcatagttat
     1201 cacatgattt tgtttcatgg cattgcggtt aagcatttcc tcttgccagt cggtgtccca
     1261 acgcatagta tcgcgacggg catcggtaaa tggcagttta tcgggtttca cgttgctgtt
     1321 ggcggcggct tcgttcatgg catccatata ttctgcggcg ttcagcacgt caacagtatg
     1381 ccatgggttt tgatatccat aatagccatc ataacttacg ttgaattttt cgcctttttt
     1441 acctttttta gtagtaatga gtacaacccc gtttcctcca cgagcaccgt aaattgctgc
     1501 tgaagcggca tctttcaaaa cttcaactga ttcgatgtcg gttgagttta ggtaatcaat
     1561 tccatagcca ttggttggta gtccgtctac aatataaagg ggttcgttgt caccattggt
     1621 tcctacccct ctaatacgta tcgaaacaaa gtctccaggt tgccctgaac tatttgtaat
     1681 t
//
LOCUS       DAOPUW010000481         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_80608_length_1676_cov_2.949414, whole genome shotgun sequence.
ACCESSION   DAOPUW010000481 DAOPUW010000000
VERSION     DAOPUW010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80608_length_1676_cov_2.949414"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PKJ43_07910"
     CDS             complement(<1..313)
                     /locus_tag="PKJ43_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72531.1"
                     /translation="MKHILLIVILVLAFVSLGWAQGSMLVVEAESGTLGADFEIRKQD
                     TYSYITSTSNDGGDSPGTATRVASYQVSLPAVGEYYLFIRLRVGADGANDDSFFYGNG
                     FG"
     gene            complement(324..1511)
                     /gene="uxuA"
                     /locus_tag="PKJ43_07915"
     CDS             complement(324..1511)
                     /gene="uxuA"
                     /locus_tag="PKJ43_07915"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HNZ72532.1"
                     /translation="MKFIYSWRWFGSNDRITLPQIRQAGAQGIVTALHQIPVGDIWTK
                     EQIAERKKIIEDNGMTWVVVESLPVSEDIKRQTGDYLKHIENYKISIRNLAENGIKTI
                     CYNFMPVLDWSRTNLKVQFHDGSQTSGFQLFIFAAFDLFILKRSGAENDYPEEVIKNA
                     SDYFSKLDDEGKQTLKDTILYGLPGSMQTYSLDEFQQHLNLYSGIDRKKLKQHLSYFL
                     NQVVPVAEECGVKMAIHPDDPPWNLLGLPRIVSTVDDLHDVVNMIKSPSNGITLCTGS
                     LGAGHFNNLPVIAKELAPSIHFTHLRNLTRDPGLNFQEDTFFDGDIDMINVVKELVAE
                     GYRREKETGEACLLPVRPDHGHQMLDDIGKENYPGYSLYGRMKNLAEIKGLTLGVIAT
                     YIP"
     gene            complement(1529..1657)
                     /locus_tag="PKJ43_07920"
     CDS             complement(1529..1657)
                     /locus_tag="PKJ43_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ72533.1"
                     /translation="MNRFGDASEIVGAVLFLVSDSASFVTGVVLPIDGGFSTFSGV"
BASE COUNT          482 a          398 c          321 g          475 t
ORIGIN      
        1 ttccaaaacc atttccgtag aaaaagctgt cgtcgtttgc cccatcggca ccaactcgca
       61 gtcgaataaa caaataatat tcgccaactg ccggtagcga aacctgataa gaagcaaccc
      121 tcgttgctgt accaggtgaa tcgccaccat cgttacttgt tgaggttata taggagtatg
      181 tatcttgttt acgaatttcg aaatcggccc ccaaggttcc gctttcggct tcaacaacca
      241 acatactccc ctgagcccat ccaagagaca caaaagccaa tactaaaatt actatcaata
      301 aaatatgctt catagaagca aatttatgga atatacgtag caataacacc aagtgttaaa
      361 cctttaattt cggcaagatt tttcatacgc ccgtataaac tgtaacccgg ataattttct
      421 ttcccgatgt catcaagcat ctggtgacca tgatcggggc gcacagggag caagcaggct
      481 tcacctgttt ccttttctct tctatatccc tcggcaacaa gttctttcac aacgtttatc
      541 atatcaatat cgccatcaaa gaacgtatct tcctgaaaat taagtccagg atcgcgggtt
      601 aaattacgca agtgggtaaa atgaattgac ggagccagct ctttagctat aaccggaaga
      661 ttattaaaat gcccggcacc aagagaaccg gtgcacaagg ttatcccgtt cgatggtgat
      721 ttgatcatat ttaccacgtc gtgcaggtcg tcgaccgtac tgacaattcg gggcaacccc
      781 agcaaattcc atggcggatc gtcgggatga atagccattt ttaccccgca ttcttcagca
      841 acaggaacca cctgattgag aaaatagctg agatgttgtt tcaatttctt cctgtcgatt
      901 ccgctataca agttcaaatg ctgctgaaac tcgtcgagcg aatatgtttg catcgacccg
      961 ggaagcccgt aaagaattgt atctttaaga gtttgctttc cttcgtcatc gagttttgag
     1021 aaataatcag aagcattttt tattacttct tcgggataat cattttcagc accagaacgt
     1081 tttaaaataa aaaggtcgaa cgctgcaaaa atgaaaagct gaaaccctga agtttgactg
     1141 ccatcgtgaa attgtacttt caggttggtt ctcgaccaat cgagcaccgg cataaaattg
     1201 taacatattg ttttaatgcc attctcagcc agattccgaa tagaaatctt atagttctct
     1261 atatgtttaa gatagtcacc ggtttgccgt ttaatatctt cacttaccgg aaggctttca
     1321 actacaaccc atgtcatgcc gttatcttca atgatttttt tgcgctcggc aatctgttct
     1381 tttgtccata tatctccaac cggaatttgg tgcaaggcag tcactatacc ctgtgccccg
     1441 gcttgtcgga tttgtggtaa ggtgatccgg tcgtttgatc cgaaccatct ccatgaatag
     1501 ataaatttca tcgcctatct ttcattaatt acactccgct aaatgtgctg aaaccgccat
     1561 cgatagggag caccacgcct gtaacaaacg aagctgaatc gctcaccagg aacaatacgg
     1621 ccccaacaat ttcactggca tcgccaaagc ggttcattgg ggtattagct aaaatt
//
LOCUS       DAOPUW010000482         1674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_80756_length_1674_cov_4.035825, whole genome shotgun sequence.
ACCESSION   DAOPUW010000482 DAOPUW010000000
VERSION     DAOPUW010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80756_length_1674_cov_4.035825"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="PKJ43_07925"
     CDS             complement(<1..852)
                     /locus_tag="PKJ43_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72534.1"
                     /translation="MKILFGISNAGLFLFIIVVIACCVAYLLYFFKKDKGEFTTTQHT
                     ILGGVRTLYSIIICFILLSPMIEIIRTRAEKPILIVGIDNSQSIRSNKNDSTEVLALK
                     NELYTALNSQFDLQLLTFGEEIKVNNEPDFSERTSDYSGFINEINKRYFNLNVGAVVL
                     VGDGVYNKGENPVQLTSKIQAPFYTMGVGDTLTSSDQAIINVAHNQNVFLNNTFQVEV
                     EASFAEYNSPSALMQVYMGNKLIQSEEIEIPQSDYYISKTFTIKAEKSGLQNISVQLT
                     PFEGEANK"
     gene            complement(1157..>1674)
                     /locus_tag="PKJ43_07930"
     CDS             complement(1157..>1674)
                     /locus_tag="PKJ43_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNZ72535.1"
                     /translation="YHEELESLVKNFPTLKRARFWMTFGQEYLTHLRVIQNIGMASIE
                     PINYEGKMIVPIQFLKAVLPNPGDLGENYTGETSIGCRIKGLKDGKDKTYYIYNNCSH
                     QAAYEETGAQGVSYTTGVPAMVGAMMYLKGLWKGEGVFNVEEFNPDPFLDELAKQGLP
                     WIELHDTDLEL"
BASE COUNT          452 a          334 c          330 g          558 t
ORIGIN      
        1 tttgtttgct tcgccttcga atggtgtgag ctgtactgaa atattttgta acccactctt
       61 ttcagccttg attgtgaatg ttttgctaat gtaatagtcg gactggggaa tttcgatctc
      121 ttccgactgg attaatttat tccccatgta cacctgcatc agggccgatg gactgttata
      181 ttcggcaaac gaggcttcaa cttctacctg gaatgtgttg tttaaaaata cgttctggtt
      241 gtgagctaca tttataattg cctgatcgga agatgttagt gtgtctccaa ctcccatggt
      301 gtagaaaggc gcctgaattt tcgaagtgag ctgaaccggg ttttcccctt tgttatatac
      361 gccgtcgcca acaagcacta ctgctcctac gtttaaattg aaatatcgtt tgtttatttc
      421 attgatgaat cctgaataat ccgatgtcct ctctgaaaaa tctggttcat tattgacctt
      481 tatttcttcg ccaaaagtta aaagctgcaa gtcgaactga ctgttaaggg ctgtataaag
      541 ttcattttta agggcaagta cttctgttga gtcattttta ttagacctga ttgattgtga
      601 gttatctatt cctacgatca gaattggctt ttcagccctt gttcgtatta tttcaatcat
      661 tggagaaaga agaatgaagc aaataataat ggaatatagt gtacgaactc cgccaagtat
      721 ggtgtgttgg gttgttgtga attctccctt gtcttttttg aaaaaataca aaaggtaagc
      781 aacacagcaa gcgatgacta cgatgataaa caaaaatagc cccgcgttcg atattccgaa
      841 tagtattttc aaagcttttc tcttcttggt tagcggcaaa tttaagaaaa tttttcaggc
      901 atgaaacgtc taataaaagg agtatccttt tttcgttcat caccggaata cgctcactcg
      961 ttaaacaaat acggtcgttc atctccaaaa atttgcaatt tggaaattta ttaaaatctt
     1021 tagcaaatta ttcaatgcat aataaacggt tattttccct tcaaaccgat gcattaaagg
     1081 tgatgtttac ccaaaaatct ctcttatttt aacgaattaa aaaagacaag gcctccgggc
     1141 cttgtctttt catatcttaa agttcaagat ctgtatcgtg aagctcgatc catggaagcc
     1201 cttgctttgc caattcgtcg aggaatgggt cgggattgaa ttcttctaca ttgaaaactc
     1261 cttctccttt ccaaagccct ttcaggtaca tcatggcacc aaccattgca gggacaccag
     1321 tagtataact aacaccctgt gctcctgttt cttcataggc ggcttggtgg ctacagttat
     1381 tgtagatgta gtaggtttta tcttttccat ctttcaatcc tttaatgcgg cagcctattg
     1441 atgtttctcc ggtataattt tcgcccagat cgccgggatt tgggagcacg gctttcaaaa
     1501 actggatggg gacaatcatt ttgccttcat agttgatcgg ctcaatgctg gccatgccaa
     1561 tattttgaat tacacgaaga tgagtgaggt attcttgtcc gaatgtcatc cagaagcgag
     1621 cccttttcaa cgtggggaaa ttcttcacca gcgattcaag ttcttcgtgg taaa
//
LOCUS       DAOPUW010000483         1669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_81138_length_1669_cov_3.199504, whole genome shotgun sequence.
ACCESSION   DAOPUW010000483 DAOPUW010000000
VERSION     DAOPUW010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81138_length_1669_cov_3.199504"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1254
                     /locus_tag="PKJ43_07935"
     CDS             136..1254
                     /locus_tag="PKJ43_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNZ72536.1"
                     /translation="MRKYLRPFGIVLAIAVFLGTFYFLYSKSKKQPDVFKIETAKFDN
                     IVKKTVATGSVLPRKEIEIKPQVSGIIDEVYLEAGQMVKNGDVIARVTIIPDMISMNN
                     AESRLNMAKLNYEDAKIDYDRQKELFDKKVIPYEEYQKAQLSFNTAREELTAAEDNLE
                     LIKKGVTKKSAKTTNTLIRSTINGMILDVPIKEGNSVIQANTFNAGTTIAVVADMNDM
                     IFEGKVDETEVGRIKEGMDILLTIGAIENQTFNAVLTYLSPKGVEENGAVQFPIKADV
                     KLQEGQFIRSGYSANASIVTDRRDSVLIIPERLLKFENDSAYVEIETATEQVFEKRHV
                     KVGLSDGINIEILEGLTVDNKIKGEKIDPKQETATSSN"
     gene            1363..>1669
                     /locus_tag="PKJ43_07940"
     CDS             1363..>1669
                     /locus_tag="PKJ43_07940"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNZ72537.1"
                     /translation="MKKVALLLITIAIFMQSQAQEKWSLQKCIDYALTNNITIKQYEI
                     NTQYQQNELDQAKNNRLPDLNASVSQSLSFGRSQQSDGTYKALNTSSTNFNIGTSALI
                     "
BASE COUNT          629 a          336 c          316 g          388 t
ORIGIN      
        1 ggttatctcc ctgccaaaag agctgttgaa ataaaaccta tcgatgcact aagggctgaa
       61 taaacaatat aaataccatc aacaagtcaa taatcaattg aaaaacaata ataaaacaaa
      121 aaaatacaag tagtcatgag aaaatattta agaccattcg gaattgtatt agcaattgct
      181 gtatttctgg gaacattcta tttcctttat tcaaaatcga agaaacaacc cgacgtattc
      241 aagattgaaa cagctaagtt tgataatatt gtcaaaaaaa ctgtagctac aggatcagtt
      301 cttcccagaa aagaaatcga aatcaaaccc caggtatcgg gaattattga cgaagtatat
      361 cttgaagccg gacaaatggt aaagaatggc gatgtaattg ccagggtaac catcatcccc
      421 gacatgatta gcatgaataa tgccgaatcg aggctcaaca tggcaaaact caattatgaa
      481 gatgccaaga ttgattacga ccgtcaaaaa gaacttttcg acaaaaaggt aatcccgtac
      541 gaagaatacc aaaaagctca gctctctttt aatacagcac gcgaggaact taccgctgcc
      601 gaagacaacc ttgaattgat taagaaaggg gtgacaaaga aatcggcaaa aacaaccaat
      661 accctaatcc ggtcaacgat taacgggatg atcctcgacg ttccaatcaa agagggaaac
      721 tctgttattc aggcaaatac attcaacgca gggacaacta ttgctgtagt tgccgacatg
      781 aacgatatga tttttgaagg aaaagttgac gaaaccgaag taggcagaat taaggaagga
      841 atggatatat tactgactat tggagcgatt gaaaatcaaa ctttcaatgc agtactcacc
      901 tacctttcgc ccaaaggcgt agaagaaaac ggtgccgttc agttccctat caaagccgat
      961 gtaaaacttc aggaaggcca gtttatccgc tcgggataca gtgccaacgc cagcatcgta
     1021 accgaccgtc gtgacagtgt cctgattatt cccgaaaggt tactcaaatt tgaaaacgat
     1081 tcagcttatg ttgaaattga aacagcaaca gagcaagtat tcgaaaaaag acatgttaaa
     1141 gttgggttat ccgacggcat caatatagaa attcttgaag ggctaaccgt tgacaacaaa
     1201 attaaaggcg aaaaaattga tccaaaacaa gagacggcta ctagttccaa ctaggataaa
     1261 gtaaacaagt tgcactgatg tggcattatc aaaaagcatt ttgccacaag gtacctataa
     1321 agaaactgaa cagattttca tcaacgtaaa atgaaaacac aaatgaagaa agttgcatta
     1381 cttttaataa ccatagcgat tttcatgcaa tcgcaagccc aggaaaaatg gtcgctgcaa
     1441 aagtgtatcg actatgcact aaccaacaac atcaccataa agcagtacga aattaacacg
     1501 caatatcagc aaaatgagtt agaccaggca aaaaacaacc gcctgcccga tttgaatgcc
     1561 agcgtgtcac aaagtctcag ttttggccgt tcgcaacaat cggatggaac ctataaagca
     1621 ctaaatacgt cgtccactaa tttcaatatt ggtacatcag ccctgattt
//
LOCUS       DAOPUW010000484         1666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_81352_length_1666_cov_3.930478, whole genome shotgun sequence.
ACCESSION   DAOPUW010000484 DAOPUW010000000
VERSION     DAOPUW010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81352_length_1666_cov_3.930478"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="PKJ43_07945"
     CDS             <1..1121
                     /locus_tag="PKJ43_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNZ72538.1"
                     /translation="SQGKVSSFEQSINGKDYFVAMAPITLSKSSTPWSLVLQLPKKEV
                     LATVNSTILISAIICLIGLFILGTIVYYLTLRLEKPLLKCVEYAREVGEGKLSETVSV
                     NTNDEIGLLADSLNQMAGHLRNIVENIAGEAHVLSRSALNLSESSKEFIELARQQENS
                     STIAETSINDLSQYLQNSTQNTESAFRLSNETTEKVNQSSVQFKESVTSVQNIANKIQ
                     VINDIAFQTNILALNASVEAARAGDSGRGFAVVAAEVRNLADRSKEAANEIVILASKT
                     KQNNETAGDMINETFNKIGEYSAIVSEMHKYTIVQQESISNIVNIVRELKSISRSNAQ
                     YANDIDHFAAELKEQSAKLNLLTSAFLIKQRKNQTNTN"
     gene            complement(1141..>1666)
                     /locus_tag="PKJ43_07950"
     CDS             complement(1141..>1666)
                     /locus_tag="PKJ43_07950"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNZ72539.1"
                     /translation="TLDTGRLFEETYKVFSRTREKYEIEIETVHPDAFELSALLKAKG
                     PFSFYQSVENRKECCNIRKVKPLRNFLSSYGVWITGLRSEQSDARSSLSLIEYDENYG
                     ILKYNPLINWTIEQVRDFVSLNHVPYNVLHDRGFPSIGCEPCTRAISPGEDIRSGRWW
                     WEQGSKKECGLHKS"
BASE COUNT          564 a          346 c          300 g          456 t
ORIGIN      
        1 tcagccaagg caaagtaagt tcatttgagc aatcaattaa tggcaaggat tattttgttg
       61 ccatggctcc aataacctta agtaaaagct cgacaccttg gtcacttgtt cttcaactac
      121 caaaaaaaga ggttttagcc acggttaact ccactatact aatttctgca atcatctgtt
      181 tgattggctt gttcattctt ggcactattg tgtattacct cacccttcgg ctagaaaaac
      241 cattattgaa atgtgttgaa tatgcccgag aagtaggcga aggcaaacta tctgaaacag
      301 taagtgtgaa tacaaacgat gagattggcc tgttggccga ttcgctaaac caaatggcgg
      361 gccacttacg aaatattgtt gaaaacattg ctggagaagc ccatgtttta tcccgctcag
      421 cactaaacct gtctgaaagt tcaaaagagt ttattgaatt agcccgtcaa caagaaaatt
      481 cgagcactat tgctgaaaca tcaattaacg acctttcgca atacttacaa aatagcactc
      541 aaaatacaga gtcagcattt cgtctgtcta acgagaccac agaaaaagtt aatcaaagct
      601 cagtacaatt taaagaatct gtaacatcgg tacagaatat tgccaacaaa attcaggtta
      661 tcaatgatat cgcatttcaa accaatattc ttgcgctaaa tgcaagcgta gaagcagcgc
      721 gtgccgggga ttcaggccgt ggttttgctg ttgtagccgc agaagtaaga aatttagcag
      781 atcgaagtaa agaagcagca aacgaaattg ttatactcgc cagtaaaaca aaacaaaata
      841 acgagacagc tggcgatatg attaacgaga cctttaacaa aattggcgaa tactcagcaa
      901 ttgtatccga aatgcataaa tacactattg ttcaacaaga aagcatctcg aatattgtaa
      961 atatagtaag agaattaaaa tcgatttcga ggagcaatgc tcaatatgcc aacgatatcg
     1021 atcattttgc ggcagagctt aaagaacagt ctgctaagtt gaatctcctc acttcagcat
     1081 ttttgataaa gcaaagaaag aaccagacaa atactaatta aatactagta atgcaacacg
     1141 ttagctcttg tgcaagccac attctttttt actgccttgc tcccaccacc agcgtcccga
     1201 cctgatatct tcaccaggag aaatagccct tgtacatggt tcgcacccaa tacttggaaa
     1261 gcctctgtcg tgtaaaacat tgtagggtac atggttcaaa gagacaaagt ctcgaacttg
     1321 ctctatagtc caatttatca acggattata ttttagtatt ccataattct catcatactc
     1381 gatcagtgaa agagacgatc gtgcatccga ttgctcgcta cgtaaaccgg ttatccatac
     1441 accatagcta gaaagaaaat tacgcaaagg cttaactttc ctaatattgc aacactcctt
     1501 acggttttca acagattgat aaaaactaaa tggtcctttg gctttaagta aagcggaaag
     1561 ttcaaaagca tcaggatgaa ccgtttcaat ttcaatctca tatttttcac gagttcttga
     1621 aaaaacttta taggtctctt caaataagcg acccgtgtcc aacgtt
//
LOCUS       DAOPUW010000485         1663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_81592_length_1663_cov_3.152363, whole genome shotgun sequence.
ACCESSION   DAOPUW010000485 DAOPUW010000000
VERSION     DAOPUW010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81592_length_1663_cov_3.152363"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..645)
                     /locus_tag="PKJ43_07955"
     CDS             complement(46..645)
                     /locus_tag="PKJ43_07955"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNZ72540.1"
                     /translation="MAIKYQFNKTSLQLLEKQLKVRERALPTIKNKESALRIEVKKAK
                     VEVQRLDELLEEKIVHYDKMIALWSEFNSALVRVDDVSLSVKKIAGVKIPVLNKIDFS
                     IAPYSLFNTPHWFPEGIELVKSLAQIGIERELVDQKLNLLEHARKKTTQKVNLFEKVQ
                     IPGYQDAILKIKRFMEDEENLSKSAQKIVKTRQEEEVEP"
     gene            complement(696..>1663)
                     /locus_tag="PKJ43_07960"
     CDS             complement(696..>1663)
                     /locus_tag="PKJ43_07960"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNZ72541.1"
                     /translation="KQPSELIATGIAGIDLNNTLVSGQKIPFFADPDQPFNQVMAMVA
                     LRAKADKIILGGMGLTNDDYLFFKNVFDNAGALDRIISFVNTTENPPVERLLVPDMAL
                     TAAEYFAVDKNESVLVLLTDMTLYADALSIVSNRMDQIPSKDSMPGSLYSDLAKIYEK
                     AVQFPDGGSITIIAVTSLSGGDITHAIPDNTGYITEGQLFLRRDTDIGKVIIDPFRSL
                     SRLKQLVIGTKTREDHPQVMNAAVRLFADAANAKTKLENGFDLTEYDERTLAYAKEYS
                     NNLLAIDVNIGTDEMLDTGWKLFAKHFSKEEVGIKQELVDKYWKS"
BASE COUNT          486 a          366 c          312 g          499 t
ORIGIN      
        1 aatcgctatg gtaaaccaga aaagcatatt tttccattgg ggttatcatg gctcaacctc
       61 ctcttcctga cgggttttta caattttctg tgccgatttc gaaaggtttt cttcatcttc
      121 cataaatcgt ttaatcttta atatggcatc ctgataacca ggtatctgaa ctttttcaaa
      181 caggttaact ttctgggttg ttttcttgcg ggcatgttcc aacaagttca acttctgatc
      241 aaccaattcc cgttcaatgc caatttgggc aaggctcttc accaattcaa taccttcagg
      301 aaaccaatgt ggagtattga aaagactgta cggcgcaata gaaaaatcaa ttttattaag
      361 gacagggatc tttaccccgg caattttctt aaccgacagg ctaacatcgt cgacacggac
      421 caatgcgctg ttgaattcac tccacaacgc gatcatttta tcatagtgaa caattttttc
      481 ttcaagaagc tcgtcgagtc gctgaacctc aaccttcgct tttttcacct cgatacgcag
      541 cgcactctcc ttgttcttta ttgttggcaa agcgcgctcc cgaaccttca actgcttttc
      601 cagtagctgc aacgaggtct tattaaattg atattttata gccatacatt ttcgaattag
      661 ttaattaccc aaaatattga atgtcaaaat attgactagc tcttccaata tttatcgact
      721 aattcctgct taattccaac ttcttcttta gaaaaatgct tagcaaaaag tttccagcca
      781 gtatcaagca tttcatcggt accaatattt acgtcgatag ccagcaagtt gttcgaatat
      841 tcttttgcat aagctaacgt ccgttcgtcg tactcagtca ggtcgaaacc attttcgagt
      901 tttgttttgg cattagctgc atcggcaaaa agcctaactg cggcattcat tacctgagga
      961 tggtcttcgc gggtttttgt accaattaca agctgcttca agcgggaaag cgaacggaac
     1021 gggtcaataa taaccttccc tatatcggta tcacgacgca agaacaattg tccttcagta
     1081 atatatcctg tattatcagg gattgcatga gtaatatcgc cacccgaaag agaagttaca
     1141 gcaataatgg taattgaacc tccatcgggg aattgaactg ctttttcata aatcttggcc
     1201 aaatcagaat ataacgaacc aggcattgag tctttagatg gaatttgatc cattcggtta
     1261 gaaacaatag acagtgcatc agcatataaa gtcatgtcgg taagcaaaac caaaacactt
     1321 tcatttttat caacagcaaa gtattcggca gcagtgagtg ccatatcggg aacgagcaaa
     1381 cgctcaaccg gtgggttctc tgtggtattc acaaaagata taatacggtc gagagctcct
     1441 gcattgtcaa aaacattttt gaagaaaaga taatcgtcat tggttagccc cataccgcca
     1501 agaataattt tatcggcttt ggcacgaagg gcaaccatag ccataacttg gttaaacggc
     1561 tgatcggggt cggcaaaaaa tgggatcttt tgtcccgaca ccaatgtatt attcaggtcg
     1621 ataccagcaa tacctgtagc aattagttca gacggctgtt tac
//
LOCUS       DAOPUW010000486         1661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_81734_length_1661_cov_4.132628, whole genome shotgun sequence.
ACCESSION   DAOPUW010000486 DAOPUW010000000
VERSION     DAOPUW010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81734_length_1661_cov_4.132628"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PKJ43_07965"
     CDS             <1..887
                     /locus_tag="PKJ43_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HNZ72542.1"
                     /translation="PEEKTSLKLNIGRIPEILLDNTDRNRTSPFAFTGNRFEFRAVGS
                     SANCASAMIALNTAVAEQLVEFKNEVDELIEKGLKKDEAIFQVLKKLIGLSKVIRFDG
                     NGYCDEWKQEAARRGLTNVTSVPEALEAYYLPSTIDMFSKLGVLSEREIESRAEVEYE
                     KFTKKIQIEARVLGDLAINHIVPTAVKYQTTLIDNVKGLKEIFGDEYKKLAGTRVELI
                     NEISGHISAIEEKVFNMVEARKKANVIENGKDKAYAYDRDVRPYLEDIRDHIDKLELI
                     VEDELWPLPKYRELLFNR"
     gene            1089..1376
                     /locus_tag="PKJ43_07970"
     CDS             1089..1376
                     /locus_tag="PKJ43_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72543.1"
                     /translation="MDARTKAIVAHLTLIGWIIAFIVNSNEKHEFATFHLRQTLGIYL
                     IGIVLGWVPVFGWICNIILFAFWLLSFIYALQGQQKTIPFGEYFQDWFKAL"
     gene            complement(1373..>1661)
                     /locus_tag="PKJ43_07975"
     CDS             complement(1373..>1661)
                     /locus_tag="PKJ43_07975"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNZ72544.1"
                     /translation="CTSLSNPTYERDGIIHYCVPNISARVSRTTTIALSNIFASIILE
                     IGEMGGIPQYIKSQKGFREGVVIYNGLLTNKDVGNKFNIPVKDLDLLMAAF"
BASE COUNT          477 a          291 c          363 g          530 t
ORIGIN      
        1 cgcccgaaga aaaaacatcg cttaaactga atattggtcg tattcctgaa attcttttag
       61 acaataccga ccgtaaccgc acatcgcctt ttgcatttac tggtaatcgt tttgaattta
      121 gagctgtagg ttcgtctgca aactgtgctt cagctatgat tgccttaaac accgcggtag
      181 cagaacagct tgtcgagttt aaaaacgaag ttgacgaact gattgaaaag ggtctgaaga
      241 aagatgaagc catcttccag gttttgaaaa aactcattgg cctgagtaag gttatccgtt
      301 ttgatggaaa tggttattgt gacgaatgga aacaagaagc tgcccgtcgt ggattaacca
      361 atgtcactag tgttcctgaa gcccttgaag cctattattt gccttcgacg attgatatgt
      421 tttcgaaact tggcgtactt tctgaaagag aaattgaaag ccgtgccgag gttgagtatg
      481 aaaagtttac caagaaaatc cagatcgagg ctcgcgtact tggcgattta gctatcaacc
      541 atattgttcc gactgctgtg aaatatcaga ctactttgat tgacaatgtg aaaggattaa
      601 aagagatttt tggcgatgag tataaaaaac ttgctggaac cagggtcgaa ttgatcaatg
      661 aaatttctgg acacatttca gctattgaag aaaaggtttt caacatggtt gaggcgcgta
      721 aaaaggccaa tgtaattgaa aatggcaagg ataaagccta cgcttacgac cgtgatgttc
      781 gcccttatct tgaggacatt cgcgaccata tcgacaaact tgagctgatt gttgaagatg
      841 aattgtggcc gttgccaaaa taccgtgaat tgcttttcaa ccggtagtaa cttagtataa
      901 tagtattttt aatagcttta aaaaggccat ctttacgggt ggccttttct tatttacgtt
      961 cagtaggttt ttaatatcat acttgttaaa cggtagtttg ccggtatttt tagtttcttc
     1021 gacgtttttc aaggaatggt gtcgacaaat ttttcctatt ttcggataaa ataaatacaa
     1081 tactaattat ggatgcacgg acaaaagcaa ttgttgcaca tttaacttta attggatgga
     1141 ttatagcgtt tattgtaaac agcaatgaga aacatgaatt tgccacattt catcttcggc
     1201 aaacccttgg tatatatctt ataggcattg tgttggggtg ggttcctgtt tttggttgga
     1261 tatgcaatat aatccttttt gcgttttggc tgctgagttt catttatgca ttgcaagggc
     1321 agcaaaagac cattcctttt ggtgaatatt ttcaagactg gttcaaggct ctttaaaaag
     1381 cggccatcag caggtctaga tctttcacag ggatattaaa tttgttacca acatctttat
     1441 tggtaagtaa gccgttataa atcaccactc cttcgcgaaa acccttttgg ctttttatgt
     1501 attggggaat tccacccatt tcgccaatct cgaggattat tgaggcaaag atattgctga
     1561 gggcaatggt ggtagtgcgt gaaacccttg ccgagatatt tggtacacag taatggatga
     1621 ttccatctct ttcgtaagtt gggttcgaaa ggcttgtgca t
//
LOCUS       DAOPUW010000487         1661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_81739_length_1661_cov_3.581569, whole genome shotgun sequence.
ACCESSION   DAOPUW010000487 DAOPUW010000000
VERSION     DAOPUW010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81739_length_1661_cov_3.581569"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PKJ43_07980"
     CDS             complement(<1..673)
                     /locus_tag="PKJ43_07980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNZ72545.1"
                     /translation="MINYEDIQKLNERIDKLEAEKKHLLEFAQQQQQTAEQLHSVIEM
                     SQTGTWEWNVQTGETVFNDQWAAMLGYELSELQPINIETWTKLTHPDDLKIAQNALDK
                     YFNKETPFYEAKFRMKHSAGHWVWVLDRGRVSQWDSNGNPLIMLGTHQEINQIQETEL
                     RLEKANRLYAVVSQVNQAIVHSKTQQKLFEEICRVAIDFGKFRMAWIGLLNSETQKIE
                     PVAVAG"
     gene            complement(1211..>1661)
                     /locus_tag="PKJ43_07985"
     CDS             complement(1211..>1661)
                     /locus_tag="PKJ43_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72546.1"
                     /translation="YIPIREVSSVDVIKCADNFELIWADKKGALPTRPIYCGSIININ
                     TVRGKGIGDVLNKFKGINRFMIPVFSTPKEYYTPNYETMKPEELNKPDLRLVLRWQPL
                     LSTDSLGIATTSFYNADNVGDMTIVVEAISKDGEIGYTTIGYKVKGK"
BASE COUNT          400 a          358 c          322 g          581 t
ORIGIN      
        1 cgcccgcaac ggctacgggc tcaattttct gggtttcgct gttgagcaac ccaatccagg
       61 ccatgcgaaa ttttccaaaa tcgatggcga ctctgcaaat ctcttcaaat aatttttgtt
      121 gggttttgct atgaacaatc gcttgattca cctggctgac aacagcatac agacggtttg
      181 ctttttcgag ccttagctcg gtttcttgaa tctggttgat ttcctggtgg gtgccaagca
      241 ttatcaatgg gtttccgttt gaatcccatt gggatacacg tcctctgtca aggacccaaa
      301 cccagtgtcc agccgagtgt ttcattctga attttgcttc atagaaaggg gtttctttgt
      361 tgaaatattt atcgagtgcg ttttgtgcaa tctttaaatc gtcgggatgg gttaacttag
      421 tccacgtttc gatgttaatg ggttgcaatt cgctaagctc gtatccaagc atggcagccc
      481 attggtcgtt gaaaacggtt tcgccggttt gcacgttcca ctcccatgtg ccggtttgcg
      541 acatttcgat aaccgagtga agttgctctg cagtttgttg ttgctgctgg gcaaactcaa
      601 gaagatgttt tttttcggcc tcaagcttgt caattctttc gttcagcttt tgtatgtcct
      661 cgtagttaat cattttaccg tgttttctta agatgctgtt aatgtatttc gtggtagaat
      721 gttaggttgt atgttcaatc atttttttgc atgttaccct ttagggattg agacccagaa
      781 cgtcgttcct ctttcactca aggtatcatt agccaaccta agctccttgt tggtatgctt
      841 taacaactga ttttcttgtc tcaacaaggc taactcgcga aaaatatcct ccatgttttt
      901 attttgttca gtcataaatt aaggtaacta atcactgctt ttgtttcgtg ttaacttaat
      961 acctgtaatt tatgcaatta aaataaactg ccgataattt tttagcaaaa aactatcatt
     1021 ttaggactaa tggtttttgc ctacagaaga aggcgggttt attgattgct aaccataaac
     1081 tctcgatgcc aacaattaca acgcagatcc tgttttaggt tgagaatcca agcattcatg
     1141 ccaatgccag tatcttttac tcgaatttgc actgaagcgt cattttctgc aacctctatt
     1201 ccaacttttc ttatttgcct tttactttat accctatcgt agtatatcct atttccccgt
     1261 cttttgaaat ggcctctacc acaatagtca tatcgcccac attgtcggca ttgtagaagc
     1321 tggtggtggc tattcccaaa ctatctgttg acagtagcgg ctgccagcgt aacaccaacc
     1381 taagatcggg tttattaagc tcttccggtt tcatggtttc gtaattgggt gtgtaatact
     1441 cttttggtgt cgagaatacg gggatcataa accggtttat tcctttgaat ttattcaata
     1501 catccccaat gcctttgccc ctaaccgtgt tgatattgat tatgctgcca caatatattg
     1561 gtcgagttgg aagagctcct tttttatcag cccaaattag ctcaaaatta tctgcacact
     1621 taataacatc aaccgagctt acttccctga taggtatata t
//
LOCUS       DAOPUW010000488         1661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_81767_length_1661_cov_2.433998, whole genome shotgun sequence.
ACCESSION   DAOPUW010000488 DAOPUW010000000
VERSION     DAOPUW010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81767_length_1661_cov_2.433998"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..770)
                     /locus_tag="PKJ43_07990"
     CDS             complement(51..770)
                     /locus_tag="PKJ43_07990"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNZ72547.1"
                     /translation="MGSFRKRNQLLVLGIFIFAGLVALGFFVGKGLRMLGAGERTVTV
                     RGLAEREVLADNIFWPIAYTVSDNDLINLNQKIEQNKRIIVEFLLSHGFSSEEISFSS
                     PSVIDVQTQLYNTDRNYSFRYLAKGFVRLNTNKLDQVLKASDNSSALLLKGVVLNSDE
                     YQTSISYDFKGLNEIKPAMIEEANINARKAGEQFAKDSKSKLGTIKEASQGLFSIQDA
                     NYNTPHIKVVRVVTNVQYYIE"
     gene            881..>1661
                     /locus_tag="PKJ43_07995"
     CDS             881..>1661
                     /locus_tag="PKJ43_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72548.1"
                     /translation="MTAYKSFKENPMYIFLVMLCIASAVGNQGWQTLFNNFAVEEAGV
                     DSVKMGIIQSLREVPGFLTFFSVYILLFIAEHRFASTSIILLGLGVMLTGFFTSLGGL
                     IFTTVLMSVGFHYFDTANQSLTLQYFQTDRVPLVLGKLRSITALSNIGVGVAIWLLSR
                     YLSYQTLFLGVGLVVVLIGVYSYILKPVDHLLPPQHKKLIFKRKYWLFYVLNFLAGAR
                     RQIFIVFAVFILVQKYHFSVIHITVLFVINNIITWLLSPYIG"
BASE COUNT          486 a          331 c          306 g          538 t
ORIGIN      
        1 gacccgaaac cttgggtgca gcattaaacg taaaacagag taaacaacat ttactctata
       61 taatattgga cgttggtaac cacccgtact acttttatat gtggggtgtt gtaattagcg
      121 tcttgaattg aaaagaggcc ttgcgaggct tcttttattg ttcccagttt actcttcgag
      181 tccttggcaa attgctcacc agcttttcgg gcattaatat tggcttcctc aatcattgct
      241 ggttttatct cgttcaaccc tttaaaatcg tatgatattg aggtttgata ttcatcgctg
      301 ttcaacacaa caccctttag cagaagtgcc gaagaattgt ctgaagcttt cagtacttgg
      361 tcaagtttat ttgtatttaa tcgtacaaag ccttttgcca gataacgaaa agaataattc
      421 cggtcggtat tataaagttg agtttgaaca tcgataacac taggcgatga aaatgaaatc
      481 tcttcggatg aaaaaccatg tgaaagaaga aattcaacaa taatcctttt attctgctca
      541 attttttgat tgaggtttat taaatcattg tcggaaacag tgtaggcgat tggccagaag
      601 atattatcgg caagcacctc acgttcagca agtccacgaa cagttacagt acgttctccg
      661 gctccaagca tcctcaagcc tttacccaca aaaaagccaa gggcaaccaa gcctgcaaaa
      721 ataaaaatac ccaaaaccaa cagctgattt ctttttctaa atgatcccat agttgttact
      781 tttcaaattc aggttaaaat taaatttaaa tttatttgta aaacaggata ttggcaggtt
      841 ttattgtacc aaacctataa gtttgcccaa aaacaaagca atgacagcat acaagagttt
      901 caaggaaaat ccgatgtata tttttttagt gatgctctgc attgcatcag ccgttggaaa
      961 tcagggctgg caaacactct ttaataattt tgctgtagaa gaagccgggg tagattcagt
     1021 gaaaatgggt atcattcaat cattgcgcga agtaccgggt ttcctcacct tcttttcggt
     1081 ttacatattg cttttcattg cagaacaccg ttttgcatca acgtcgataa ttttactagg
     1141 attaggagtc atgctcaccg gctttttcac ctcgttgggc ggcttaattt tcacaacggt
     1201 gctcatgtct gttggctttc actatttcga cacagccaac caatcgctta cccttcaata
     1261 ttttcaaacc gatcgtgttc cactagtgct gggtaaacta agaagtataa cagccctctc
     1321 gaatattgga gttggagtag caatttggtt gctctcgaga tacttatcgt atcaaacgct
     1381 ttttcttggc gtaggattag tggttgttct cattggagta tactcatata tactaaaacc
     1441 agttgaccat ctgcttcccc ctcaacataa gaaactgatt ttcaaaagaa aatactggtt
     1501 attctatgtg ctgaatttct tagccggagc ccgcaggcaa atttttattg ttttcgctgt
     1561 ttttatcttg gttcagaaat atcatttttc ggtaatacat atcactgtgc tatttgtgat
     1621 taacaacatt atcacatggc tattaagtcc atacattggg a
//
LOCUS       DAOPUW010000489         1660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_81809_length_1660_cov_3.360125, whole genome shotgun sequence.
ACCESSION   DAOPUW010000489 DAOPUW010000000
VERSION     DAOPUW010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81809_length_1660_cov_3.360125"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..940)
                     /gene="folD"
                     /locus_tag="PKJ43_08000"
     CDS             complement(62..940)
                     /gene="folD"
                     /locus_tag="PKJ43_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNZ72549.1"
                     /translation="MILIDGKKIAAEIKAEIALEVKNITEQGGKRPHLAAILVGHDGG
                     SETYVASKIKDCEEVGFVSSLFRFEDDATEAEVLQKVYDLNNDADVDGFIVQLPLPKH
                     ISEQKIIEAIDPKKDVDGFHPINVGRMVIGLPAFVSATPDGIVELLKRYNIETSGKNC
                     VVIGRSNIVGRPMSILMSQKALNTTVTVAHSQTKNLKEVCANADILIAALGSPEFVKA
                     DMVKEGAVVIDVGTTRVPSAETKSGFKLKGDVKFDEVAPKCSYITPVPGGVGLMTRVS
                     LLKNTLLAAKKTIYKS"
     gene            complement(953..>1660)
                     /locus_tag="PKJ43_08005"
     CDS             complement(953..>1660)
                     /locus_tag="PKJ43_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNZ72550.1"
                     /translation="IKKGIDPNEILFVVDSMTGQDAVNTAKEFNDRLDFDGVVLTKLD
                     GDTRGGAALSIRAVVNKPIKFVGTGEKMEALDVFHPKRMADRILGMGDIVSLVEKAQE
                     QFDEQEAIRLQKKLAKDQFNFNDFLAQINQVKKMGNLKDLAGMIPGMGKALKDVEIDD
                     NAFVHVEAIIYSMTPAEREDPSLMNGTRRKRIADGSGTSIQEVNRLLKQFTETRKMMK
                     MVAQGKRFTPPARNVRR"
BASE COUNT          448 a          402 c          297 g          513 t
ORIGIN      
        1 taaaaacatt tatgtagata ccgtacaatt taagaaaaaa gccccaatgc caatcggtta
       61 attacgattt ataaattgtc ttttttgctg ctaaaagagt atttttcagc aacgaaacac
      121 gagtcatcag tccgacccca cctggcacag gggttatata tgagcacttt ggcgcaactt
      181 catcaaattt aacatcacct ttcagtttaa aacccgattt ggtttcagcc gatggaaccc
      241 gggtagtacc cacatcgata actacagctc cttctttaac catatcggct ttaacaaact
      301 ctggagaacc aagtgccgca attaaaatat cggcattggc acatacttcc ttaaggttct
      361 ttgtttggct atgagccaca gttacagtgg tattcaatgc cttttgcgac atgagtatgc
      421 tcattggcct tccaacgatg ttactacgtc caattaccac gcagtttttt ccagacgttt
      481 caatgttata acgcttcagc aattcaacaa tgccatcggg agtagccgat acaaaagccg
      541 gcaaaccaat aaccatgcgg ccaacattaa ttggatgaaa gccatcgaca tcttttttcg
      601 ggtcaatcgc ttctataatt ttttgttccg aaatatgttt aggcagaggg agttgaacga
      661 taaaaccatc aacatcggca tcgttattca ggtcataaac tttctgcaaa acttctgctt
      721 ctgtagcatc gtcttcaaac cggaataacg atgagacaaa gccaacttcc tcacaatctt
      781 ttattttact tgccacataa gtttcactgc ctccatcgtg cccaaccaaa atagcagcca
      841 aatgaggcct ttttcctcct tgttcagtaa tgttcttcac ctcgagggca atttcagcct
      901 ttatctcggc tgcaattttt tttccgtcta tcagtatcat acgagtatat ttttatcggc
      961 ggacattgcg ggctggcgga gtaaaccttt tgccctgagc caccattttc atcattttac
     1021 gggtttctgt aaattgtttc aataagcggt taacttcctg tattgaagtc ccggaaccat
     1081 cggcgatcct tttacgccgg gtgccgttca tcaaggacgg atcttcgcgc tctgccggtg
     1141 tcattgagta aatgattgct tcaacatgaa caaatgcatt atcatcaatc tcaacatcct
     1201 tcagtgcttt acccattccg gggatcatcc cggccaagtc tttcaggttt cccatttttt
     1261 taacctgatt gatttgtgcc agaaaatcgt tgaaattaaa ttggtctttt gccaatttct
     1321 tttgtaaacg gatagcttcc tgctcatcga attgttcttg cgccttttca acaagagaaa
     1381 caatgtcacc catgcctaaa atcctgtcgg ccatacgttt cggatggaaa acatcgagcg
     1441 cttccatttt ttctccggta cctacaaact tgataggttt gttcactaca gccctaattg
     1501 acaaggctgc accaccacgg gtatcgccat cgagcttggt aagcactacc ccatcaaaat
     1561 cgagcctatc gttaaattct ttagcggtat ttacggcatc ctgtcctgtc attgaatcga
     1621 caacaaacaa aatttcattg ggatcgattc cttttttgat
//
LOCUS       DAOPUW010000490         1656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_82097_length_1656_cov_3.074953, whole genome shotgun sequence.
ACCESSION   DAOPUW010000490 DAOPUW010000000
VERSION     DAOPUW010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82097_length_1656_cov_3.074953"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PKJ43_08010"
     CDS             complement(<1..499)
                     /locus_tag="PKJ43_08010"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418300.1"
                     /note="catalyzes the formation of
                     N(1)-(5-phospho-D-ribosyl)glycinamide from
                     5-phospho-D-ribosylamine and glycine in purine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNZ72551.1"
                     /translation="MKILIIGSGGREHALGWKIKQSTKVEKIYFAPGNAGTATIGENL
                     AIGVCDFSSIKKAVLSNQIDMVLVGPEVPLTEGIFDFFASDSELKNIPVIGPSKEGAK
                     LEGSKDFAKEFMMKYNIPTARYQTFSKGEASLGAEFMKTLTPPYVLKADGLAAGKGVV
                     IPETIG"
     gene            complement(511..1227)
                     /locus_tag="PKJ43_08015"
     CDS             complement(511..1227)
                     /locus_tag="PKJ43_08015"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNZ72552.1"
                     /translation="MTKRINLRVHLPEWMNRLYPKAIWRMPTGEKTVYLTFDDGPIPT
                     VTCAILDILKERGIKATFFCVGDNVVKHPEIFCQILEQGHAVGNHTHNHLHGMKTSNS
                     DYISNIHKAAQHINSRLFRPPYGLMKGLQYKAIVDQYDIVMWDVISCDYDPDISPEKC
                     FQNVVDFVRDGSIITFHDSIKSEKNVLTALPRVIDYLLSQGYSFRKITSQAPLPEKVK
                     KPDEHFKQLQKSTEKIRKSA"
     gene            complement(1271..>1656)
                     /locus_tag="PKJ43_08020"
     CDS             complement(1271..>1656)
                     /locus_tag="PKJ43_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72553.1"
                     /translation="QRLADALLRQGDKGRCVNVLDKCMEEIPTSKVPNDYFSLLIAQT
                     YLNAGATGKAGAMASEMSKEFTDELVYLFSLKSDDVRDVQENIRSNLYYLNELMTLAE
                     KLGNKELQSKIKADFELYYGKFMAI"
BASE COUNT          429 a          339 c          344 g          544 t
ORIGIN      
        1 cgccgatggt ttctggaata acaacccctt tcccggcagc tagcccatca gccttcagaa
       61 cataaggagg agtaagggtt ttcataaatt cggcacccaa tgatgcttcg cctttagaaa
      121 atgtttggta acgtgctgta ggaatgttgt atttcatcat aaactctttg gcaaagtcct
      181 tgctgccctc gagttttgcc ccttctttag atggaccaat tacaggaata ttttttaatt
      241 cggaatctga ggcaaagaaa tcgaaaatac cttcggtgag aggaacttct ggaccaacta
      301 aaaccatgtc tatttggttc gaaagaacag ctttttttat agaactaaaa tcacataccc
      361 caatggcaag gttttctcca attgttgctg ttccagcatt tccgggggca aaatatattt
      421 tttccacctt ggtgctttgt tttattttcc atccgagggc gtgttctctt cctcctgaac
      481 caattattaa tattttcatt atcaaaagtg ttatgcgctt tttcttattt tttctgttga
      541 tttttgaagt tgcttgaagt gttcgtccgg ttttttaact ttttcgggta gtggggcctg
      601 agacgtaatt ttgcggaatg aatatccttg gctaagcaga tagtcgatta ccctgggtag
      661 ggcagtgaga acattctttt ctgattttat ggagtcgtga aaagtaataa ttgaaccgtc
      721 gcgaacaaag tcaacaacat tctgaaaaca cttttctgga ctaatatcgg ggtcataatc
      781 gcaactaatc acatcccaca taacaatatc gtactggtcg acaatagctt tgtattgcag
      841 tcctttcatc aaaccatatg gtggcctgaa aagcctggaa ttgatatgtt gagccgcttt
      901 atgtatgttg gaaatgtagt cggaattact ggttttcatc ccgtgtaaat ggttatgggt
      961 atggttgcca acagcgtgtc cctgctccaa aatctgacaa aatatttcgg gatgcttcac
     1021 aacattgtcg cctacacaaa agaaagtagc ttttataccc cgttccttca ggatgtcgag
     1081 aatggcacag gtaacggtgg gtattggccc atcgtcgaat gtaaggtaaa ccgttttttc
     1141 ccccgtaggc attctccata ttgctttagg ataaagccga ttcatccatt cagggaggtg
     1201 aacccgtaag tttattcttt ttgtcatccg atgttgcttg gtgctactat cttattccgc
     1261 aaattccgga ttaaatcgcc atgaattttc catagtataa ctcaaaatca gctttaattt
     1321 tgctttgcag ttccttgttg ccaagttttt ctgcaagggt catcaattca tttagataat
     1381 agaggttact acggatattt tcttgtacat cacgaacatc atctgatttt agcgagaaca
     1441 aataaaccag ttcgtcggta aattctttcg acatttcgct tgccattgca ccagcctttc
     1501 cggttgcacc tgcattgaga taggtttgtg cgataagcag tgagaagtaa tcatttggta
     1561 ctttgctggt aggaatttct tccatgcatt tgtcgagaac atttacgcaa cggcctttat
     1621 ctccttggcg gagaagggca tcggcgagcc gctgga
//
LOCUS       DAOPUW010000491         1654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_82259_length_1654_cov_2.926829, whole genome shotgun sequence.
ACCESSION   DAOPUW010000491 DAOPUW010000000
VERSION     DAOPUW010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82259_length_1654_cov_2.926829"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PKJ43_08025"
     CDS             complement(<1..204)
                     /locus_tag="PKJ43_08025"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663092.1"
                     /note="Catalyzes the reversible hydrolysis of the amide
                     bond within dihydroorotate. This metabolic intermediate is
                     required for the biosynthesis of pyrimidine nucleotides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNZ72554.1"
                     /translation="MKTLIKNATIVNEGRTFTGSVLIENDLIACILENNDQLKADITI
                     DASGQLLLPGVIDDQVHFREPGLT"
     gene            complement(263..979)
                     /locus_tag="PKJ43_08030"
     CDS             complement(263..979)
                     /locus_tag="PKJ43_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNZ72555.1"
                     /translation="MKDSVIIIPTYKEKENIESIIRYVFNLPVQFHILVIDDNSPDGT
                     AAIVKTLMTEFPEKLHLIERPGKMGLGTAYIRGFQWSIENGYEYIFEMDADFSHNPDD
                     LIRLREACLQGADLAIGSRYVSGINVVNWPLKRVLMSYFASKYVRIITGMKVHDSTAG
                     FVCYTSRVLKALDLNNIRMKGYGFQIEMKFTSWKHGFKVVEIPIIFIDRQKGTSKMSG
                     GIFSEAFFGVIKMKLRSIFN"
     gene            1247..>1654
                     /locus_tag="PKJ43_08035"
     CDS             1247..>1654
                     /locus_tag="PKJ43_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HNZ72556.1"
                     /translation="MVKIGKTSTLEIIKFTDQGAYLDGGPYGEILLPNRYVPANCAEG
                     DDVDVFVHFDSEDRIVASTDFPAVMVDQFGYLQVVGVSSFGAFLDWGLPKDLFVPFRE
                     QTVKMEKGKWYVVRVYVDNVTGRIAASAKYKKFL"
BASE COUNT          441 a          303 c          336 g          574 t
ORIGIN      
        1 ggttaagccc ggttcgcgaa aatgaacctg atcgtcaatt acaccgggta aaagaagctg
       61 cccacttgca tcaatagtta tgtctgcttt tagttggtcg ttattttcca atatgcaggc
      121 aataagatcg ttttcgatga gcacagaacc ggtgaaagtc cggccttcat taactatggt
      181 tgcatttttt attaaagtct tcattgtagt tattttgact ggtataaaaa aagctccctg
      241 gggagctgtt caagtattat ttttaattaa aaatactcct taatttcatt ttaataacac
      301 caaaaaatgc ttcgctgaaa atgcccccac tcatttttga ggttcccttc tgtctgtcga
      361 tgaatatgat aggaatttca actactttga acccatgttt ccatgatgtg aatttcattt
      421 cgatctggaa cccgtaacct ttcatgcgaa tattgttcaa gtcgagggct tttagtaccc
      481 gcgaagtata gcaaacaaac ccagcggttg agtcgtgtac tttcatgccg gtaattatac
      541 gcacatattt tgatgcaaaa tacgacatta agactctttt tagtggccag ttaactacat
      601 ttataccaga aacatagcga gacccaattg ccaagtctgc cccttgaagg catgcttcgc
      661 gtaaacggat aaggtcgtcg gggttgtgtg agaagtcagc atccatttcg aagatgtatt
      721 catatccgtt ttctattgac cattgaaatc ccctgatata ggcggttcca agccccattt
      781 ttccggggcg ttcaattaga tgaagttttt cagggaattc ggtcatcaat gtttttacaa
      841 tagctgctgt accgtcgggc gaattatcgt cgattacgag gatgtgaaat tgcaccggaa
      901 gattgaaaac ataacgaatt atgctttcga tattttcctt ctccttgtat gtgggtataa
      961 ttattacact atctttcatc gtaccttctc tttttttgat cttaaacaaa aatactattt
     1021 tcttgcttat tttacctatg taactttttt ttaggacaaa ttgattgtcg gccaatttgt
     1081 aaagcacagg gaatcagttg ctaatttcca ggaatgctag tttgtattga gattcttatg
     1141 cagcatttct tttgtagaat gtctttttaa ataactctca taattggttt ctcccggaga
     1201 acaaaacttt cgttactttt gcgtcttaaa atattgaatt acagttatgg tgaaaattgg
     1261 aaaaacatct actcttgaga taattaaatt taccgatcaa ggagcatatc tcgatggggg
     1321 gccttatggt gaaatattgc tgccaaaccg ctatgtacct gctaattgtg ccgaaggcga
     1381 tgatgttgat gtttttgttc atttcgactc tgaagatcgt atagttgctt caaccgattt
     1441 tcctgcggtt atggttgatc agtttggtta tttgcaggtt gttggcgtga gttcgtttgg
     1501 cgcttttctc gattggggtt tgcccaaaga tttatttgtg ccttttcgtg agcagactgt
     1561 caaaatggag aaagggaagt ggtatgttgt acgtgtttat gttgataatg tcaccggccg
     1621 aattgcggct tctgctaaat ataaaaagtt tcta
//
LOCUS       DAOPUW010000492         1652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_82420_length_1652_cov_3.039449, whole genome shotgun sequence.
ACCESSION   DAOPUW010000492 DAOPUW010000000
VERSION     DAOPUW010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82420_length_1652_cov_3.039449"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /gene="secA"
                     /locus_tag="PKJ43_08040"
     CDS             complement(<1..601)
                     /gene="secA"
                     /locus_tag="PKJ43_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420827.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, there are paralogous
                     proteins that have been found to be nonessential but do
                     function in secretion of a subset of exported proteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNZ72557.1"
                     /translation="MGIAQLLGKFFGTKSDKDIKEIMPIVETIKKEYDRISKYNNDEL
                     RAETLLVKKKISDFVKAEEDEIAQLRLKAENVSIEESEAIYDQIDKLTASIDEKLENI
                     LNQVLPVAFSIVKETARRFVETEWVEVTASDYDRDLAALYDHVEIKGEKAYWHNRWIA
                     GGNEITWDMVHYDVQLIGGVVLHQGKISEMATGEGKTLVA"
     gene            814..>1652
                     /locus_tag="PKJ43_08045"
     CDS             814..>1652
                     /locus_tag="PKJ43_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72558.1"
                     /translation="MKPFFCLCLMGLLCFVAPHEGFSSDMGKNFKTSIYTRAYEVQKM
                     ADQQWLESTWKTISEQMHVDKIYLETHRDLLIVDENTLKKAISFFKSKGLDVAGGITY
                     TIDESNQFQTYCYTNPEIRAKAQEVIEYTARFFDEIILDDFFFTSCKCEHCVKAKGGK
                     SWTEYRLDLMTKAANDLIITPAKKINPNAKVIIKYPNWYDHFHGLGFNLETEPKMFDG
                     VYTGTETRDAELSNQHLQPYLSYLAFRYFYNLKPGKNGGGWVDTGGMRYYDRYAEQLW
                     LTI"
BASE COUNT          485 a          364 c          305 g          498 t
ORIGIN      
        1 tagcaacaag cgttttacct tcaccggtag ccatctcaga aatctttccc tgatgtagca
       61 caacgccccc aatgagctgc acatcgtagt gaaccatgtc ccaggtgatt tcatttccac
      121 cggcaatcca acggttgtgc caatatgctt tttctccttt aatttctaca tggtcgtaaa
      181 gagcggccaa atctcgatca taatcactag ctgtaacttc tacccattca gtttcaacaa
      241 agcggcgtgc tgtttctttc acaatggaaa aggccacagg aagcacttga ttcagaatat
      301 tttccaattt ttcgtcaatg ctggctgtga gtttgtcaat ctgatcgtaa atagcttcac
      361 tttcttcgat gctgacgttt tcagccttca gacgcaattg tgcaatctca tcttcttctg
      421 cttttacaaa atcgctgatc ttctttttaa ccagcaaggt ttcggcacga agttcgtcgt
      481 tgttatattt cgaaatccgg tcatattctt ttttgatagt ctcaactatc ggcattattt
      541 ccttgatgtc tttgtcagat tttgtgccaa agaactttcc aagtaactga gctataccca
      601 tgtttttatt ttttactatt tcgtttgtta taaattcaag gttccgtaaa ggaacttttt
      661 agcacgacaa aaataattta attgcgggag gaataaaacg ttaagccatt ttatttgccc
      721 ccgctttcga tttacataaa gtttaccggt atcttcaatt ttttctgtca attatctacc
      781 tttgaaattt cacaaactta aaatctacta ggtatgaaac cattcttttg tttatgtctc
      841 atgggcttgc tgtgttttgt agctccgcat gaaggctttt cttcggatat ggggaaaaat
      901 tttaaaacat cgatttacac acgagcgtac gaagttcaga aaatggctga ccaacaatgg
      961 cttgaatcga catggaaaac aatttcggaa caaatgcatg tcgataaaat ttatcttgaa
     1021 acccatcgtg acctgctaat tgttgatgaa aacacgctca aaaaagcaat ctcttttttc
     1081 aagtcgaaag gacttgatgt agcgggggga ataacctaca ccatcgacga aagcaaccaa
     1141 tttcaaacct attgctacac taatccggaa attagggcta aagcacagga agttattgag
     1201 tacactgccc gtttcttcga cgaaattatc ctcgacgatt tctttttcac cagctgcaaa
     1261 tgcgaacatt gcgtgaaagc aaaaggcgga aaaagctgga cagaataccg gctcgaccta
     1321 atgaccaaag cggcgaacga cctcatcata acccctgcaa aaaagataaa ccccaacgca
     1381 aaagtcatta ttaagtaccc aaattggtac gaccactttc atggacttgg gtttaacctc
     1441 gagactgaac caaagatgtt cgatggggtt tacacaggaa ccgaaacacg cgatgcagaa
     1501 ctaagcaacc aacatttaca accttatctc agctacttgg ctttcaggta tttttacaat
     1561 ttaaagcccg gaaaaaacgg tggcggatgg gtggatacgg gaggtatgcg ttattatgac
     1621 cgctatgccg aacaactttg gctcaccata tt
//
LOCUS       DAOPUW010000493         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_82502_length_1651_cov_2.719925, whole genome shotgun sequence.
ACCESSION   DAOPUW010000493 DAOPUW010000000
VERSION     DAOPUW010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82502_length_1651_cov_2.719925"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1651
                     /locus_tag="PKJ43_08050"
     CDS             <1..>1651
                     /locus_tag="PKJ43_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNZ72559.1"
                     /translation="AASNEDWKTIHSSSNPVFSTKEIYNRLVSVNTKMPFYPVPDSAA
                     IQKFINIAKGKHTTDSIYVFNAFPPYLEVLGTGFFDNDIAPGINYEYRVSKIEKTGKA
                     LETKLIKALSFPGKPNNFKATPIEVMPQGNKINIRFKITGDSKPTGFRVYRQVYLQTA
                     FREIHPSTSYMNTPGGITMLMTDTLVVPRAIYNYLVVPFDVLGNDGLVSDTIKVNNVA
                     EYREVPMLRKLEAISLDRELSIKLRWEATNVQNLRGISIYRSLNFDDGYTLYSRVSAT
                     DSTFIDRNVQPITNYYYYLVFNGAYGDSPESAKVIGMVKGLKEPVLPPQQVKTESTDA
                     GNVITWRGIENDTKGYYVFRGEGYENPTEQISGLITCDSMNVAFIDSASTLKPGQVYC
                     YAVRSVNKSNLLSNFSETAIAEVLKPQLPTPLNLEVRNFNGAALLIWEDMKAISPYIT
                     GYRVLRQEKGKEKVQISTSYNLPINRFVDTSLVRGIPYSYFIQAEGIANSESKPGAPY
                     EFSLSLLEPVPPAGIRATKTDIGIVLYWDLPAVEGLTGFNI"
BASE COUNT          528 a          357 c          329 g          437 t
ORIGIN      
        1 ttgcagccag taatgaagat tggaaaacaa tacattcttc atcgaaccct gttttttcaa
       61 caaaagaaat atacaaccgg ttggtatcgg taaacaccaa aatgcctttt tacccggtac
      121 ccgattctgc tgccattcag aaatttataa acattgcgaa aggaaaacat accaccgatt
      181 ctatctatgt tttcaatgca tttcctccgt acctcgaggt gcttggaact ggctttttcg
      241 acaacgatat agccccgggt atcaactatg aataccgcgt ttcaaaaatt gaaaaaacag
      301 gcaaagccct ggagacaaaa cttataaaag cactatcgtt cccgggtaaa cccaataatt
      361 ttaaagccac cccaattgag gtgatgcccc agggaaataa aatcaacata cgtttcaaaa
      421 ttacaggcga ttcaaaacca acagggttcc gtgtttaccg tcaggtgtac cttcaaaccg
      481 ctttccgcga aattcatcca tcaacatcat acatgaatac gcccggtgga attacaatgc
      541 ttatgaccga cacccttgtg gtacccaggg caatatataa ttatttggta gttccgttcg
      601 atgtgttggg caacgacgga cttgttagtg atacgataaa ggtaaataat gtagcagaat
      661 acagagaggt acccatgctc agaaaacttg aagccatttc gctcgacaga gaattgtcga
      721 taaaactcag gtgggaagcg acaaacgtac aaaaccttcg gggaatttct atttatcgca
      781 gccttaattt cgatgacgga tatacgctat attcaagggt tagtgcaacc gactcaacct
      841 ttatcgaccg taatgttcaa cctataacaa actattacta ctacctggtg ttcaatggtg
      901 cttacggaga tagccctgaa tcggccaaag taattgggat ggtaaagggg ttgaaagaac
      961 ctgttctgcc tccgcaacaa gtaaaaactg aatcgaccga tgccgggaat gtaatcacgt
     1021 ggagaggaat cgagaacgat acaaaaggat attatgtttt caggggtgaa ggctatgaaa
     1081 accctacaga acaaatttca ggactaataa cctgcgacag tatgaatgtt gcttttatcg
     1141 attcggcatc aacattaaaa cccggtcagg tttattgtta cgctgttcgt tcggttaata
     1201 aaagtaattt gctgagcaac ttttcagaaa cagctattgc tgaagttttg aaaccccaac
     1261 tgccaactcc attaaacctt gaagtacgta actttaacgg agctgccctc ttaatttggg
     1321 aagacatgaa agccatttct ccatacataa ccggatacag ggttttacgt caggaaaaag
     1381 gcaaggaaaa agtacaaata tcaacaagct ataatttgcc aataaaccgt tttgtcgaca
     1441 cttcgcttgt tcgtggcata ccatattcat actttattca agccgaaggg attgccaaca
     1501 gcgaaagcaa gccgggtgct ccatacgaat tttcactttc actgcttgaa ccggttcctc
     1561 ccgcaggaat tagagcaact aaaaccgaca ttggaattgt tttatactgg gatttaccgg
     1621 cagttgaagg tcttactggc ttcaacattt a
//
LOCUS       DAOPUW010000494         1650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_82589_length_1650_cov_2.885893, whole genome shotgun sequence.
ACCESSION   DAOPUW010000494 DAOPUW010000000
VERSION     DAOPUW010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82589_length_1650_cov_2.885893"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /gene="nrdD"
                     /locus_tag="PKJ43_08055"
     CDS             complement(<1..1219)
                     /gene="nrdD"
                     /locus_tag="PKJ43_08055"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HNZ72560.1"
                     /translation="MELKIIKRDGKAELFSVEKIKTAISKAFLSVGSFATEEVLCNIL
                     SRVSINNGIPVEEIQNQVEIALMAERYYAVAKSYMLYRQKHAEDREVSEKLKFLMDYC
                     DASNAATGSKFDSNANVENKNMATLIGELPKSNFIRLNRRLLTDRIKDMYGKELADRY
                     IELLNNHFIYKNDETSLANYCASITMYPWLINGTSAIGGNSKAPTNLKSFCGGFINMV
                     FMVSSMLSGACATPEFLMYMNYFIGLEFGEDYYKETGMIVDLSKKQRTLDKIITDYFE
                     QIVYSINQPTGARNFQAVFWNISYYDRYYFESLFDNFYFPNGSKPHWESFSWLQKRFM
                     KWFNQERTRAVLTFPVETMALLTENGEPKDKEYGDFTAEMYAEGHSFFTYLSDNADSL
                     SSCCRLRNEITDNG"
     regulatory      complement(1338..1537)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          473 a          361 c          311 g          505 t
ORIGIN      
        1 atccgttgtc ggtaatttcg ttacgcaggc ggcaacaact gctcaacgag tcggcgttat
       61 cgctaaggta agtaaaaaac gaatgccctt cagcatacat ttcggcagtg aaatcgccat
      121 attctttgtc ttttggttcg ccgttttctg tcaataaagc catagtctca accgggaatg
      181 tcaacacggc acgtgtacgt tcctggttaa accacttcat gaagcgcttt tgcagccagc
      241 taaacgactc ccagtgtggc ttactaccat tgggaaaata gaaattgtcg aacaggcttt
      301 caaaataata gcggtcgtaa tacgaaatat tccaaaaaac agcctgaaaa ttacgtgccc
      361 ctgtgggctg gtttatcgag tacacaattt gctcaaagta gtcggtaata atcttatcga
      421 gtgtccgttg ttttttcgac aggtctacaa tcatcccggt ttctttgtaa tagtcctccc
      481 caaattcaag accgataaag tagttcatgt acatcaggaa ttcgggcgtt gcacaggctc
      541 cactcaacat actcgacacc atgaatacca tattgataaa acctccacaa aacgatttca
      601 ggttagtggg tgctttcgag tttcctccaa tggccgaagt tccgtttatg agccaagggt
      661 acatggtaat acttgcgcag taattggcca aactggtctc gtcgtttttg taaatgaaat
      721 gattgttcag gagttcaata tatctgtcgg ccagctcttt accatacata tctttaatgc
      781 ggtcggttaa taatctgcga tttaggcgta taaaattaga tttaggtaac tctccaataa
      841 gggtagccat atttttgttt tccacgttgg cattcgaatc gaacttactc cctgtggccg
      901 cattactggc atcgcaataa tccatcagga attttaattt ttcgctcacc tcacggtctt
      961 cggcatgttt ttggcgataa agcatatacg acttggcaac agcataatag cgctcagcca
     1021 taagtgcaat ttctacctga ttttggatct cctccacagg aattccgttg ttgatgctca
     1081 cacggctcag aatgttgcaa agcacctctt cggtagcaaa actacctacc gataaaaaag
     1141 cttttgaaat ggccgtttta attttttcaa cagaaaacag ttctgctttc ccgtctcttt
     1201 taatgatttt cagttccata caacttatgc tacaggtatc acgttcaaca acttattttt
     1261 caaccaatgg agcattctgt aaatagcagg aataaagcac ccgaaagaaa ccttattcat
     1321 tgcttttcac ccgaaagcta cgaattaaat ttgcaggcag gtcttctggc ttgtccggtt
     1381 ttgacgcctt cccgtctgtc atttgacaaa cagtggcaaa gtgtgccaaa actattgaat
     1441 ggacttacag ctgcgggtac agcccccgtt tttcacggag ttcccttttc attttattcc
     1501 cgaataggaa tataaaacct gtaaattttg tgtttgtaaa ggaaatgaaa aaactttgtc
     1561 agaataagaa tacggctgag aatttataaa cactttttgt taataacaaa ccacaaactg
     1621 taatcatttt tattataaat acctataaat
//
LOCUS       DAOPUW010000495         1646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_82888_length_1646_cov_3.311754, whole genome shotgun sequence.
ACCESSION   DAOPUW010000495 DAOPUW010000000
VERSION     DAOPUW010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82888_length_1646_cov_3.311754"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..561)
                     /locus_tag="PKJ43_08060"
     CDS             complement(1..561)
                     /locus_tag="PKJ43_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNZ72561.1"
                     /translation="MERKNLASLYTQSFRDNWDLPLFSDYEGETFCYKDVALSIQTIK
                     SVFTNIGIAKGDKIAIYGRNSSNWGKIFLACASYGVIAVPILPDFKPENVHNIVNHSG
                     AMFLFAARSLYSTLNIDEMPSLKGVIAIEDFAILKAIDENLQKFTSQGLSLMKDGHID
                     REAFEFEPVDSDEMMILSYTSGSSGF"
     gene            796..>1646
                     /locus_tag="PKJ43_08065"
     CDS             796..>1646
                     /locus_tag="PKJ43_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72562.1"
                     /translation="MKKNIKNTILFLSLLFVGSLLIIASVNYFFKVQRQKMADAKQEE
                     LSSIANIKVEQIAEWYDDELLDAEIIVDNTHFIQIVKQWILSDGTYSNAEVSLMLSQL
                     AKEHGYHSVTIFSDDLHRQVSSGVVVPHFNDVTLTTLKQCLLSEKTLSTDLFVCELDG
                     DVFIDFVAPLFFPDISRKTMVVFRHDPNKFLFPFVETWPVQSRTSESLLLRNEADSVL
                     FMSNLRHKSGEVMKMKLPLVVNSCVEVNAAMGQEGISVGKDYRGENVLASLNPVPGTR
                     WVLVTKT"
BASE COUNT          492 a          291 c          341 g          522 t
ORIGIN      
        1 tcagaagcca gaggagcccg aagtatatga aaggatcatc atttcatcgc tgtcgaccgg
       61 ttcgaactca aaagcttcac gatcgatgtg tccatctttc attaaggaaa gtccctgact
      121 ggtaaatttt tgcaggttct catcgatggc tttaaggatg gcaaaatctt caatggcgat
      181 tacccctttc aaactaggca tttcgtcaat attgagagtt gagtaaagtg aacgggcggc
      241 aaataagaac attgcccccg agtgatttac aatattgtga acattttcgg gtttaaagtc
      301 aggcagaata ggaacggcaa ttaccccata cgaagcacag gccaggaaaa tcttacccca
      361 attagaagag tttcggccat aaatggcgat tttatcccct ttcgcaatac caatatttgt
      421 aaaaacactt tttatagttt gaattgaaag ggcgacatct ttataacaaa acgtttcgcc
      481 ttcatagtcg gaaaacaatg gtaaatccca attgtctcgg aatgattgcg tgtataagct
      541 ggccagattc ttgcgttcca tactgtataa tttaaagtgt tattaagtta aatctttatg
      601 caaaagcacg ataagatact tacttaaatg aaaaaaaaca ttattttttg caaaaataaa
      661 ctaactgtac aaaaaaggga ttgttttttg ttttttttac tgaatagtgc tagatcaatt
      721 gtcggctgtt ttttttaaat cataagagaa acacatgcct atttttctta tatttacact
      781 caaaatgttg ttgctgtgaa aaagaatatc aagaatacta tactttttct gtctctactg
      841 tttgttggta gtttgctaat tattgccagc gttaattatt tttttaaagt acagcggcag
      901 aagatggccg atgctaagca agaagagctt tcgtcgattg ccaatattaa agttgagcaa
      961 attgccgagt ggtatgatga cgaacttctc gatgcagaaa taattgtaga caatactcat
     1021 tttattcaaa ttgtgaagca atggattttg tctgatggta cctatagcaa tgctgaagta
     1081 tcactcatgt taagccagtt ggcaaaagaa catggatatc attctgttac tatattttct
     1141 gatgatttgc acaggcaagt ttcttcgggt gtagttgttc ctcatttcaa cgacgttacc
     1201 ttgacgactt tgaaacaatg cttgctctcg gagaaaacac tttctaccga tttatttgtt
     1261 tgtgagttag atggggatgt tttcatcgac tttgtggcac cgcttttctt tcccgacatt
     1321 tcccgaaaaa cgatggtggt atttaggcac gatcctaata agttcctttt tccctttgtt
     1381 gaaacttggc cggtacaaag ccggacctcg gagtcgctat tgttacgaaa tgaggcagac
     1441 tcggtgttgt ttatgtctaa tttgcggcac aaatctggag aggtaatgaa aatgaaattg
     1501 ccgcttgttg ttaactcttg tgttgaagtt aatgcagcta tggggcaaga aggcatatca
     1561 gttggaaaag attatcgtgg agaaaatgta ctggccagcc tgaacccagt gccgggaacc
     1621 cgttgggtat tggtaaccaa aacaga
//
LOCUS       DAOPUW010000496         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_82947_length_1645_cov_4.091195, whole genome shotgun sequence.
ACCESSION   DAOPUW010000496 DAOPUW010000000
VERSION     DAOPUW010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82947_length_1645_cov_4.091195"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PKJ43_08070"
     CDS             complement(<1..807)
                     /locus_tag="PKJ43_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72563.1"
                     /translation="MNKMNKAKLNTVLKKHIPFIIVFLMIMLFNGCKDDRTQYMESKP
                     LSKIEQKTTINFIGHWYKEGKREDLVRNFVREYSLINQEVNINLKFPEEVYYNREDLY
                     SNRKFVVSVLKEKKPSWDIVRLNSEYFEISTLLGDPDWPKKYLVDFSEIEEFRQGTLP
                     ELLTEKAKEEWNGIIPGPYIEGQFWALWCNLNVAKKLGIEIKQYGMTYSDFLGYIKAV
                     YNYNQRNPSEYITPIYVSADWPTARAIAVQLFAWLLDSPEEFFLDKITNNR"
     gene            complement(931..>1645)
                     /locus_tag="PKJ43_08075"
     CDS             complement(931..>1645)
                     /locus_tag="PKJ43_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNZ72564.1"
                     /translation="RIAQGAYKVNGSSKQISSISFKVAQGSMQQAASAEEVSSSVEEM
                     AAVIQDSNSNAKQTQLISDKAAEGITELIEREQESLKYIKEISEKISIVNDIAFQTNI
                     LALNAAVEAARAGEQGRGFAVVAGEVRRLAENSRIAADEITKLSSKSVTITNNAHDFL
                     MKLAPEIEKTSKLVDDISISSSELSNGASQINSAIQELNLVIQQYSATAEDMARNSEV
                     LKNEALELEQSIRFFNIEE"
BASE COUNT          402 a          333 c          318 g          592 t
ORIGIN      
        1 cctgttgttg gttattttat cgagaaagaa ttcttcgggg ctatcgagca gccaggcaaa
       61 gagctgaaca gctatggccc gggctgtggg ccagtcggcc gacacgtaaa ttggcgtaat
      121 atattcgctc ggattacgtt ggttgtaatt ataaacggct ttaatatatc ctaagaaatc
      181 ggaataggtc atcccgtatt gtttaatttc tattcccaac ttctttgcca cattcaaatt
      241 gcaccagagc gcccaaaatt gaccttcaat gtatggcccg ggtattattc cgttccattc
      301 ttcttttgct ttctctgtta ataattccgg cagggttcct tgcctgaatt cttcaatttc
      361 actaaagtct acgaggtatt tcttgggcca atcgggatct cctaaaagag ttgaaatctc
      421 aaaatattca ctgttaaggc ggacgatatc ccatgatggt tttttttctt ttagtacaga
      481 aactacgaat ttgcggttgc tatataaatc ttcgcgatta taatatacct cttcagggaa
      541 cttaaggttg atgtttactt cctgatttat taagctatat tctcttacga aattccggac
      601 aaggtcttcg cgtttccctt ccttatacca atgtccaatg aaatttatag tggttttttg
      661 ctcaatcttc gacagcggct tactttccat gtattgtgtc ctgtcgtctt tgcacccatt
      721 gaataacata atcatcagaa atacaatgat gaaaggaata tgctttttga ggacagtatt
      781 tagttttgct ttattcattt tgttcatcga ttttttcttt tactttgaaa aaacgggact
      841 ttaacaattc caaattaacc aattatgata atcccgtttt gtaaattttt gtttaccgta
      901 ttattgcggt tttgagtcga attttatatg ctattcttca atattaaaga acctaatgct
      961 ttgttcaagc tcaagggctt catttttgag aacttcagag tttcgtgcca tatcttcagc
     1021 agttgctgaa tattgctgaa taaccagatt gagttcctgg attgcgctat tgatttgcga
     1081 agctccattg ctcaattcgc tgctcgatat tgatatgtca tcgacaagtt tcgaggtctt
     1141 ttcgatttct ggtgcgagct tcatcaagaa gtcgtgggca ttgttagtaa tggttaccga
     1201 ttttgaagat aactttgtga tctcgtcggc tgcaatgcgg ctgttttcag ctaatcgacg
     1261 aacttctccg gcaacaactg caaaccctcg gccctgttct cctgcacgag cggcttcaac
     1321 ggctgcattg agtgccaaaa tgttggtttg aaaggctatg tcgtttacta tggaaatctt
     1381 ttctgagatt tctttgatat atttcaagct ttcctgttct cgttcaataa gttcagtaat
     1441 tccttcggca gctttgtctg atattagttg tgtttgtttc gcattactgt tactgtcttg
     1501 tattactgca gccatttctt caactgagga agaaacttct tctgccgagg ccgcctgttg
     1561 catcgagcct tgtgcaactt taaacgaaat tgagcttatt tgtttactac tgccattaac
     1621 tttgtaagcc ccctgggcaa tacgt
//
LOCUS       DAOPUW010000497         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_82969_length_1645_cov_2.867296, whole genome shotgun sequence.
ACCESSION   DAOPUW010000497 DAOPUW010000000
VERSION     DAOPUW010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82969_length_1645_cov_2.867296"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="PKJ43_08080"
     CDS             <1..1180
                     /locus_tag="PKJ43_08080"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNZ72565.1"
                     /translation="ALKAGSQELVKIDFIFEAGSWYQSRNLVAGLSNAFLNQGSTKYT
                     AQEIAEVFDSRGAYLQLSADQQFGNVSILTLTKYLNDILDVTADIIKHPTFPEKEVAT
                     HIAKRKQQFIVENNKVKTLALKKFTQVLFGEGHPYANTNTVNDFDRLTREDVRLFHSE
                     NYRFNRCKIVVAGNFDNSLNSLLDAYFGDHFNDGDTIQPAHKPQPAVEKQHFIKKDDA
                     VQSAVRIGKQLVNRDHPDFHGLSILTTILGGYFGSRLMANIREEKGYTYGIGATIVTF
                     QHASYITISTEVGTDVCKQAVAEIYSEIEKLIHEPVQAEELDVVKNYLMGDMLRNFDG
                     VFALSGSLRTLVESALDYSHYETYIDELKNITPERLQWLAQSYFQPDSWYEVVAGKNS
                     "
     gene            complement(1342..>1645)
                     /locus_tag="PKJ43_08085"
     CDS             complement(1342..>1645)
                     /locus_tag="PKJ43_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72566.1"
                     /translation="APRLSLQAGAGITSFGAGINYHLKPQINSSMISLSYWHQGIGDS
                     YTQSLLGPSFVFRARKIFTAQLGLGYLLEEGPAWPENTAHPSAMLLYSIGIYLPIK"
BASE COUNT          512 a          366 c          333 g          434 t
ORIGIN      
        1 ggcactgaag gcaggcagtc aggaattggt aaaaatcgat ttcatttttg aggcaggttc
       61 atggtaccag tcgcggaatt tagttgccgg gcttagcaat gcctttctca accaaggctc
      121 aacaaaatat actgctcagg aaattgccga agtattcgat tcgcggggag cctatttgca
      181 gctttctgcc gatcaacaat ttgggaatgt aagcatactc actctaacaa aatatcttaa
      241 tgatatactg gatgttactg ccgacataat aaaacatcct accttccccg aaaaagaagt
      301 tgccacgcat attgctaaac gaaaacaaca atttattgtt gaaaataaca aggtaaaaac
      361 ccttgcgcta aaaaaattca cccaggtgct tttcggcgaa ggacatccct acgccaacac
      421 caacacagtt aacgactttg atagactaac acgagaagat gtcaggttgt ttcattctga
      481 aaattatcgt ttcaaccggt gcaaaattgt ggttgccggc aatttcgaca actctctaaa
      541 cagcttactc gatgcatatt tcggcgatca tttcaacgat ggcgatacta ttcaaccagc
      601 gcataagcct caaccagcag ttgaaaaaca acattttata aaaaaagacg atgctgtgca
      661 gtcggccgtt cgcattggta aacaactagt taacagagat catcccgatt ttcacgggct
      721 ttcgatcctc accacaatac tcggtggcta tttcgggtca aggctaatgg ccaatatccg
      781 tgaagaaaaa ggctatacct acggcattgg tgcaacaatc gttacatttc aacatgcctc
      841 gtacattacc atatcaaccg aagtaggtac cgatgtttgc aaacaggcag tcgccgaaat
      901 atattccgaa atagaaaaac ttattcatga accggttcaa gcggaagaac tggatgtggt
      961 gaaaaactac ctgatgggtg acatgttgcg aaatttcgat ggggtttttg cgctgtcggg
     1021 cagcctgagg acacttgtag aatcggccct tgactactcg cattatgaga catacatcga
     1081 tgaactaaaa aatattaccc ctgaacgact gcaatggctc gcccaatcat attttcaacc
     1141 cgatagttgg tatgaagttg tagccggcaa aaattcttag ctattaacct atgcttgatg
     1201 ttttttcgcg aatctcctaa acctcatata catagtaacg aaattcagca ctgtgggtaa
     1261 gacaattaaa caagattcct gtcacatttc tggaaaataa accagccccg acgttttggc
     1321 agggctggtt caaattattc tttactttat cggcaaataa atacctatag aatacaacaa
     1381 catagcagat ggatgcgcag tgttctcagg ccaggctggg ccttcttcaa gcaaataacc
     1441 aagccccaat tgggcagtga atatttttct ggccctgaaa acaaacgatg gccccagtaa
     1501 actttgtgta tagctatcgc caataccttg atgccaatag cttaatgaaa tcattgaact
     1561 gttaatttgt ggtttcaggt ggtaattaat accagcccca aaactagtaa tgccagctcc
     1621 ggcctgtagg ctcagtcgtg gagca
//
LOCUS       DAOPUW010000498         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_83101_length_1643_cov_4.227960, whole genome shotgun sequence.
ACCESSION   DAOPUW010000498 DAOPUW010000000
VERSION     DAOPUW010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83101_length_1643_cov_4.227960"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(378..551)
                     /locus_tag="PKJ43_08090"
     CDS             complement(378..551)
                     /locus_tag="PKJ43_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72567.1"
                     /translation="MKQKKIELDVDFIGGQGALTVEEEKSLSDFFKQRKMTFQKPQNK
                     ISTKTVKHSKTTA"
     gene            complement(563..1084)
                     /locus_tag="PKJ43_08095"
     CDS             complement(563..1084)
                     /locus_tag="PKJ43_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72568.1"
                     /translation="MKNDKSTGLDFIVDKLTNSIENVVTGDSFKTEVSVLTKSELKLV
                     TKKNGWVFDWRKELKEPSRDIYKLTINGNPSVIQGLMSIEVKEDHVFMHLVESAPYNK
                     GKSKMYSGVPGNLVAFACKLSFQRGHEGNVSFLSKSQLVDHYIKTLGAFHFGGRVMII
                     ETQSALKLIDRYF"
     gene            1121..>1643
                     /locus_tag="PKJ43_08100"
     CDS             1121..>1643
                     /locus_tag="PKJ43_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72569.1"
                     /translation="MNCLEFALSACAITDGTPINLTSCRFIDGISYAFPKGTMIEPDN
                     FLVLASSSFSFSQRYGMAASGEYEGQLSNGGERITLVGAIGDTLISVKYDNKQPWPVT
                     PDSLGFSLVPAVNNLSADWNDGKNWRASSRIAGSPFADDVPENIPGIIINEILANSEA
                     PQVDAIELYNPENT"
BASE COUNT          453 a          344 c          304 g          542 t
ORIGIN      
        1 gtcctaaaat atggctacag gttaaacatt aatttatgtt tgctacaaat acctcgttag
       61 gggttttata ccgattgaag aaatgtaaca gtccaatctt cgttcactcg attggccttg
      121 acatttctat tcatcggcat tatacctgag tggttttgat ctgttttgat attgcatctg
      181 gtataatacc cttaagatag gcaaagtatt ttattgttat atagcatatt ggcacttttt
      241 gttgccagcg ttgctttttc ggtgttcccc cgtcccgatg gcatcgggat tgcgggctat
      301 actggctgtt gtcggaacga aaacggcaat tgatatatag tttttattat tgccagtgct
      361 ttgtcttgat aggtcaatta tgcagttgtt ttactatgtt ttacggtctt tgttgaaatt
      421 ttattctgag gcttttggaa agtcatttta cgttgcttga aaaaatcact cagcgatttt
      481 tcttcttcaa ctgtcaaagc accttgaccg ccaataaagt caacatctaa ttctattttc
      541 ttttgtttca taattaactg ttttaaaagt atctgtcaat aagttttaat gcggattgag
      601 tttctataat cattactcgt cctccgaaat gaaatgcgcc aagagttttt atatagtgat
      661 caacaagctg tgatttagac aagaatgaaa catttccctc gtgccctcgc tgaaatgaaa
      721 gtttgcaagc aaatgcgact aaattaccgg gaactcctga atacattttt gacttgcctt
      781 tattgtaagg tgcactctcg actaaatgca taaaaacatg gtcttctttg acttcaatac
      841 tcataagtcc ctgaataacc gaaggattac cattaatcgt caatttataa atgtctctgg
      901 atggttcttt caattctttt ctccaatcga atacccaacc atttttcttc gtgaccaatt
      961 tcagctcaga ttttgtaaga acagaaactt cagtcttgaa actgtcacct gttacaacat
     1021 tctcaattga gtttgtcagc ttatcaacaa tgaaatccaa ccctgtgctt ttgtcatttt
     1081 tcattcagct aatttacaaa aaataagtca aatctattaa ttgaactgcc tcgaatttgc
     1141 gctctcagca tgcgccataa cggacggaac accgattaac ctcacttcct gtcgctttat
     1201 cgatgggatc agctatgcat ttcccaaagg aacaatgatc gaacccgata attttcttgt
     1261 acttgcatca agtagtttta gtttcagcca acggtatggt atggctgcgt cgggcgaata
     1321 cgaaggacaa cttagtaatg ggggcgagcg tattaccctt gttggtgcca ttggcgacac
     1381 actcatctcg gtaaaatacg acaacaagca accctggcct gttacgcccg atagtctggg
     1441 attctctctt gtccctgcag taaacaacct ttctgccgat tggaacgacg gcaaaaactg
     1501 gagggcttca tcgcgaattg ccggatcgcc ttttgctgat gatgttccgg aaaatatccc
     1561 cggaattatt atcaatgaga tattagccaa ttcagaagcc ccacaggtcg acgcaattga
     1621 actctataac cccgaaaata cgg
//
LOCUS       DAOPUW010000499         1640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_83376_length_1640_cov_2.304101, whole genome shotgun sequence.
ACCESSION   DAOPUW010000499 DAOPUW010000000
VERSION     DAOPUW010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83376_length_1640_cov_2.304101"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1618)
                     /locus_tag="PKJ43_08105"
     CDS             complement(170..1618)
                     /locus_tag="PKJ43_08105"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNZ72570.1"
                     /translation="MFNFKIITRTLGFLLIGEGLLMVAALLVSLLYQEGDTIHFIHSA
                     LITIVTGVILFLFTRKASPTIGKREGYIIVSIVWVLFSFFGSLPFMLSGSIPALHDAF
                     FETMSGFTTTGASILDNIEELPHGILFWRSLIQWIGGMGIVVLSLAILPAFGIGGISL
                     YAAEAPGITTEKINPKIKDTAKILWNTYLVFTVVETIMLMFGGMSLFDSVCHSFTTMA
                     TGGFSTKQASVAYWNSPYIHYVITAFTIIAATNFSLTYFSFTGSPGKLFKNEEFKLFI
                     SIILVFTLIITTGLLLTTDYSLEKAFRNSIFQVVTIISTTGYVTDDFMLWHPFLTYVL
                     FLLFFVGGSTGSTSGSIKVMRIGLLLKNSYYELRRLIHPNAVIPVRFNNRAVNNQIIN
                     NVLAFFFFYIAVFFICSLIFMLFVDDYETSIGAVATCLGNIGPGLGQVGPAFSFSQIP
                     DAGKWFLSFIMLLGRLELFTVLVIFSPSFWRR"
BASE COUNT          540 a          317 c          336 g          447 t
ORIGIN      
        1 tgtcgcagcc catgctataa acggtgtcgg taggataaat aattattcct ccgtttttta
       61 gcgtatcgac gatgtaattc aactttcgct ggtcgatgtt ttcttcgtaa agtttaatca
      121 gcattctgag atttttaact cccaaaaata gcaaataaac tgcgaaatgt tatcgtctcc
      181 agaaactggg agaaaagatt accagaacgg taaatagctc aagccttccc aaaagcatta
      241 taaacgaaag aaaccatttt ccggcatcgg gtatttggct gaagctaaag gcggggccta
      301 cttgccctaa tccggggcca atgtttccca gacatgtagc taccgctcca atcgatgttt
      361 cgtagtcatc tacaaaaagc ataaatatga ggctgcaaat gaagaataca gcaatgtaga
      421 aaaagaaaaa ggcgagtaca ttgtttatta tctggttgtt gactgctctg ttgttaaatc
      481 gtacaggaat aactgcgttg gggtgaataa gcctgcgtaa ttcgtaataa ctgtttttaa
      541 ggagaaggcc aattcgcatc acctttatcg aaccactggt tgatccggtc gatccgccta
      601 caaagaatag caaaaagaga acataggtaa ggaatgggtg ccataacata aagtcgtcgg
      661 tgacatatcc tgttgttgat attattgtaa caacctgaaa tatagaattt ctaaaggctt
      721 tctccagact ataatcagtt gtcaataaca agcctgtggt aataatcaac gtaaagacaa
      781 gtataatact tatgaataat ttaaactctt cattcttaaa caactttcct ggcgagcctg
      841 taaacgaaaa gtaggtgaga gagaaattag tcgcggcaat aatggtaaat gctgttataa
      901 catagtgaat gtatggcgaa ttccaataag caacactggc ttgtttggtc gagaatcccc
      961 ctgtggccat ggtcgtgaat gagtggcaaa cagaatcgaa caaactcata cccccaaaca
     1021 tgagcattat tgtctctacc actgtgaaaa ccagataggt gttccaaagt attttggctg
     1081 tatctttaat tttggggtta atcttttcag ttgtaattcc tggtgcttct gccgcataga
     1141 gcgatattcc acctatgcca aaggccggta aaatggccaa agaaagcaca actatgccca
     1201 taccgccaat ccattggatc aaacttcgcc agaaaaggat tccatgaggc agttcttcta
     1261 tattgtcgag aattgatgcc cctgtggttg tgaaacccga catggtttcg aaaaaagcat
     1321 cgtgaagagc aggaattgaa ccacttagca taaaaggcaa acttccgaaa aaagaaaaca
     1381 acacccatac gatactcaca ataatatatc cttcacgttt gccaatagtt ggcgaggctt
     1441 ttcgcgtgaa aaggaataaa ataacaccgg ttacgatggt tattaatgct gaatgaatga
     1501 aatgaattgt atctccttct tggtataaca gcgagactaa aagggctgca accataagaa
     1561 ggccttctcc gatcaataag aagcccagag tgcgggttat tattttgaaa ttaaacatga
     1621 aggctattta aaaaatttat
//
LOCUS       DAOPUW010000500         1634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_83803_length_1634_cov_4.986067, whole genome shotgun sequence.
ACCESSION   DAOPUW010000500 DAOPUW010000000
VERSION     DAOPUW010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83803_length_1634_cov_4.986067"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..>1634)
                     /locus_tag="PKJ43_08110"
     CDS             complement(212..>1634)
                     /locus_tag="PKJ43_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNZ72571.1"
                     /translation="LRMLFLVKIVVFINKKEMNFPIIPYIMAADMVSRADAGFVNIWG
                     LQDCAETIHEFADEDQKNRYLPRVCQGETMAMDLTEPDAGSDLQAVELKATFDEKKGK
                     WMLNGVKRFITNGDGDIALVLARSEEGTHDGRGLSMFIYDKRDGGVTVRRIEHKMGII
                     GSPTCELVFKNAPSELVGQRRMGLIKYVMALMNGARLGIAAQSVGVSEAAYREALAYA
                     NEREQFGKPIIQFPAIYEMISVMKAKLDASRSLLYETGRFVDMYKSYYHISNERALDK
                     DEREEMKLYQKLADIYTPLAKGMTSEFCNQNAYDAIQVHGGSGFMKDYPVERIYRDAR
                     ITTIYEGTTQLQVVAAIRGVTTGGYLAQIREYEKTQLKPELEQYRRILIGLTEEFAEA
                     VERVVATKDNEYIDFHARRLVEMAGTIIMSYLLILDANRDMENLRSAKVFNKIARQVV
                     HGHGEFIRGTSVSDMANYKFELE"
BASE COUNT          455 a          399 c          318 g          462 t
ORIGIN      
        1 tcgtcgccat aatcaaacgt gtagcgaagg ctacgaagaa tgtcgttgtt gttcataatt
       61 gatctcgaat attaaactcc ctgcgagcag tttttctgca aatatattga aaattatcgg
      121 ccttcataaa aatgaaaaac cccgctaaca gcgaggcttt tcattttatt attttgggaa
      181 agagcagtac ccaatgaata gggtattctt tctattccaa ttcgaattta taattggcca
      241 tatcgctaac cgaggttcca cgaatgaatt caccatgacc atgaaccact tgccgggcaa
      301 ttttattgaa cacttttgcc gagcggaggt tctccatatc gcggtttgca tcgagaatta
      361 gcagatagct cataatgatt gttcctgcca tttcgaccaa gcggcgggcg tgaaaatcga
      421 tgtattcatt atctttagtt gccacaacac gttcaacagc ttcggcaaac tcttcggtca
      481 acccgataag aatgcgccga tattgttcca actctggttt aagctgggtt ttttcgtatt
      541 cacggatttg ggctaaatac ccaccggtgg ttacccctct gatcgcagca actacctgca
      601 actgcgtggt tccttcgtag atagttgtaa tacgggcatc acgataaatc ctttcaacag
      661 gataatcttt catgaaacct gaacctccgt gaacctgtat ggcatcgtaa gcattctggt
      721 tacagaattc ggaagtcatt ccttttgcca gcggagtata aatatcggcc agcttttggt
      781 acaacttcat ttcttcgcgt tcgtctttgt cgagtgcacg ttcgttcgat atatgataat
      841 aagacttgta catgtcgaca aaacgccctg tttcatacaa taatgaacga gaagcatcga
      901 gttttgcctt catcaccgaa atcatttcgt agattgccgg aaactgaatg attggttttc
      961 cgaactgttc gcgctcgttg gcataagcca gcgcctcacg atatgcagcc tcagaaacgc
     1021 caacagactg agcagcaata cccaaacgag caccattcat aagagccatt acatatttta
     1081 tcagccccat gcgacgctgt ccaaccaatt cggaaggagc atttttgaac accaattcac
     1141 acgttggcga accaatgatc cccattttat gctcgatacg acgaacagta acacctccgt
     1201 cgcgtttatc gtaaataaac atcgacaagc cacgcccatc gtgagttcct tcttcagagc
     1261 gggcaagaac cagcgcaata tcgccatcac cattggtaat gaagcgtttt acaccgttga
     1321 gcatccattt tccctttttt tcgtcaaatg ttgcttttaa ttcaacagcc tgtagatcgg
     1381 agcctgcatc gggttcggta aggtccatcg ccatggtttc tccctggcaa acccggggta
     1441 aataacggtt cttctgatct tcgtcggcaa actcgtgaat agtttcggca caatcctgca
     1501 agccccaaat attcacgaaa cccgcatcgg cacgcgaaac catatcggca gccattatat
     1561 agggaataat cggaaaattc atttcttttt tattaataaa aacaacaatc ttcaccagaa
     1621 acaacattct taat
//
LOCUS       DAOPUW010000501         1633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_83933_length_1633_cov_2.615970, whole genome shotgun sequence.
ACCESSION   DAOPUW010000501 DAOPUW010000000
VERSION     DAOPUW010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83933_length_1633_cov_2.615970"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="PKJ43_08115"
     CDS             <1..1255
                     /locus_tag="PKJ43_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNZ72572.1"
                     /translation="WTNNTWVNPPDEFKDSFIKGYSRRNGQFMRNFYWSQPALNFGFA
                     KPTEPWMLPTDHPDIVAMKEELKNVLRFWMNMGADGFRADMAGALVKNANIQGNDQFF
                     NARDEATKQFWQEIRQLMDREYPGSFMVAEWSGPKDALDKAFHSDFFHWFAGYNDLVQ
                     KESWRILNGYSEGNSFFDKEGKGNIQTFLDSYLEQYNATKDKGYITIPLGNHDNARIN
                     VNRTDKELEMIYAFGLTMPGIPFIYYGNEIGMKQLYNMPNVEGAYPPRSGNRTPMQWS
                     TAKNLGFSTCDPASLYLPVDTASNAPCVEVQEKNPKSLLNQTRRLILLKRTEPALKAY
                     AEFVPVYAGTNKYPFIFTRANGNEVIICVFNPASRAETAQFKLNFRAKKIKLLAGDHL
                     AITSKGLNYTVKTPPVSYAIYKVSK"
BASE COUNT          488 a          304 c          356 g          485 t
ORIGIN      
        1 ctggacaaac aacacttggg taaacccacc cgatgagttt aaagattcat ttattaaagg
       61 atacagcaga cgtaacgggc aatttatgcg aaacttctat tggagccaac cagcacttaa
      121 ttttggtttt gcaaaaccca ctgaaccgtg gatgcttcct acggatcatc ccgacattgt
      181 ggcgatgaaa gaggaattga aaaatgtact ccgtttctgg atgaatatgg gagccgatgg
      241 tttccgtgcg gatatggccg gagcacttgt gaaaaatgca aacatacaag ggaacgacca
      301 attttttaat gctcgcgatg aagcaaccaa acagttctgg caagaaataa gacaactgat
      361 ggatcgtgaa tatcccggtt catttatggt tgccgaatgg tcggggccta aagacgccct
      421 cgataaggct tttcattccg actttttcca ttggtttgca gggtacaacg atctggttca
      481 aaaagaaagc tggcgaattc tcaacgggta ttccgaaggg aatagtttct tcgataaaga
      541 aggaaaggga aatattcaaa ccttccttga tagttatctt gagcaataca atgccaccaa
      601 agataagggc tatattacca ttccgctggg taatcacgat aatgcccgaa taaatgtaaa
      661 ccgaaccgac aaggaacttg agatgattta tgcgtttggg cttaccatgc cgggaattcc
      721 atttatctat tatggcaatg aaattgggat gaagcagttg tataacatgc ccaatgtaga
      781 aggagcttat ccgccgcgat cggggaacag gactccaatg caatggtcaa cagcaaagaa
      841 ccttggcttc tcaacttgtg acccagcctc gctttattta cctgtcgata cagcttctaa
      901 tgctccttgt gtcgaagtac aggaaaagaa cccaaaatcg ttgcttaatc aaacgcgtcg
      961 gcttattttg ctgaaacgaa ccgaacctgc gttgaaagcg tatgcagagt ttgttcctgt
     1021 gtatgcaggt actaacaaat atccgttcat atttacccgg gccaatggca acgaggttat
     1081 tatttgtgta tttaatcctg catcaagggc tgaaactgcg cagtttaagc tcaatttcag
     1141 ggcaaagaaa ataaaattac ttgccggcga tcatttggca attacgtcta aagggttgaa
     1201 ctatacggtt aaaactccgc cggtgagtta tgcaatttac aaagtaagca aatgatagaa
     1261 gggctgaata gtattggtcg tttgaggaga tcaaaaaata taaacagtta ttctgaatgt
     1321 atattttccc gatgattgtg tacccgtact tttgcattgt taagatttag tatacgtatt
     1381 aataacattt tgattgttag ttttaattct gttatttaaa ctaactttgc gatgcttgtt
     1441 taatttgttt tatttgtata attgcataca gtttgcaact aacagttaca agctaatatt
     1501 acattgaatt tttggtttta gtgataagag tgaattattt agacgcctgg ccggtcggtt
     1561 ttcgatatgc cgggcgtctt gtttattaac ctgagttcga ctttattttt gagttcagac
     1621 cgcttgtaaa cag
//
LOCUS       DAOPUW010000502         1631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_84076_length_1631_cov_3.072970, whole genome shotgun sequence.
ACCESSION   DAOPUW010000502 DAOPUW010000000
VERSION     DAOPUW010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84076_length_1631_cov_3.072970"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PKJ43_08120"
     CDS             <1..548
                     /locus_tag="PKJ43_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72573.1"
                     /translation="GMIYIWFNGLFYLIQNQYETASDELKNLFSANYYDLNTLRFGGD
                     VRYSSKSFSLGLNGNYYSYSSNSNVTITQRPAFDAQLTSLFNIGSSIKVRLDAQVIGS
                     RKAEIMVVANKNDGSAPAISQEIHTIKTAIGISAGADYAYNKKLSFSLDVRNMINQNY
                     ELWHGYNTQGILVLLGARYTF"
     gene            836..>1631
                     /locus_tag="PKJ43_08125"
     CDS             836..>1631
                     /locus_tag="PKJ43_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010423371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ72574.1"
                     /translation="MSELENKNGINGNGNGNNGYSADSIQVLEGLEAVRKRPAMYIGD
                     VNEKGLHHLVYEVVDNSIDEALAGYCKNIEVIINEDNSITVTDDGRGIPVDFHEKEQK
                     SALEVVLTVLHAGGKFDKDSYKVSGGLHGVGVSCVNALSIYLKAEIHRDGKVWVQEFS
                     QGKPLGDIRTEGDSDKTGTIITFKPDDSIFLTTSYKYEILSARLRELAFLNAGLSLSL
                     IDKRETDDDGNFKKEEFFSEEGLKEFVEYLEGVREKLIDETIHISTE"
BASE COUNT          521 a          293 c          345 g          472 t
ORIGIN      
        1 tgggcatgat ctatatttgg ttcaatgggc ttttttattt gattcagaat caatacgaaa
       61 cagcctcaga tgagcttaaa aacttatttt cagccaatta ttacgactta aatacccttc
      121 gctttggtgg agatgttaga tacagcagta agtcgtttag tttagggcta aacgggaatt
      181 attacagcta ttcaagcaat tcgaacgtaa ctataaccca aaggcctgca ttcgatgcgc
      241 agcttacttc gcttttcaat ataggctcat caattaaggt ccggctcgat gctcaggtaa
      301 ttggttcacg caaagccgaa attatggttg tggcaaataa aaacgatggt agcgcaccag
      361 ctatcagtca ggaaatccat acgataaaaa cggctatcgg gataagcgcc ggagccgatt
      421 atgcctacaa taaaaagttg tcgttttcgc tcgatgtacg gaacatgata aaccagaact
      481 acgaattgtg gcatggctat aatacacagg ggatattagt actgttaggt gctcgttaca
      541 ccttctgatt attaggtgtt tttcaactta atttgttgat aacttttgcc tttcactcac
      601 tgaatagtta ggcagaaagg ttatattttc ctatatttga gcgcaaattt ttaggaacag
      661 aaaatcgttt gtaatttatt gaaaatcata attaataaat gattttttct ctttgttttt
      721 ttctatatgt tctacaggaa ataaagatca tcgggcctaa ttttttgaat acaaaaaata
      781 ccatcttgaa agcgatggaa aatttgaatt ttttagatga agaaaataac aaaccatgag
      841 tgaattagaa aacaaaaacg gtatcaacgg aaacggcaat gggaataacg ggtattctgc
      901 cgatagtatc caggtacttg aagggttgga agctgtgcgt aagcgcccgg caatgtatat
      961 tggcgatgta aacgaaaaag gcttacacca cttggtttat gaagtggtcg acaactcaat
     1021 cgacgaagct cttgccggat attgcaaaaa tattgaagta atcataaacg aagataattc
     1081 gatcactgta accgacgacg gacgtggtat tccggtcgac tttcacgaaa aagaacaaaa
     1141 atcggctctc gaagtggtac taactgttct tcatgccgga ggtaaattcg acaaggactc
     1201 ttacaaagtt tccggtgggt tgcacggggt tggtgtatca tgtgtcaatg ccctgtcgat
     1261 atatttgaaa gctgaaattc atcgcgatgg taaagtttgg gttcaggaat tctctcaggg
     1321 caaacctctt ggcgacatcc gtacagaagg tgattcagac aaaaccggga caattatcac
     1381 attcaagccc gacgactcaa tatttttaac cacttcgtat aaatacgaaa tactctcggc
     1441 aaggcttcgt gagctggcat ttctcaatgc agggctgagc ctttctctta tcgataaaag
     1501 agaaactgat gacgatggta atttcaaaaa agaggaattc ttctcagaag aaggcttgaa
     1561 agagtttgta gaatacctcg aaggtgttcg tgaaaaactt atcgacgaaa ccattcatat
     1621 ttcaactgaa a
//
LOCUS       DAOPUW010000503         1626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_84494_length_1626_cov_2.361553, whole genome shotgun sequence.
ACCESSION   DAOPUW010000503 DAOPUW010000000
VERSION     DAOPUW010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84494_length_1626_cov_2.361553"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            457..>1626
                     /locus_tag="PKJ43_08130"
     CDS             457..>1626
                     /locus_tag="PKJ43_08130"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNZ72575.1"
                     /translation="MKARLFWLTGMSFFFYGWWDWRFLFLIIFSGLIDYYAALAMVRW
                     EKYRKLLLFLSMAGNIGSLSVFKYSGFIAQNIDMLFGLTGAFSLAGNIPPFFLVTPVG
                     ISFYTFQSMSYTLDVYHRKFTPTRDVIHFFAFLSMFPQLVAGPIIRAKEMLPQLLVVK
                     KVESDEKWAGFRYVVKGYFMKMVIADNLAPLVVTAFSAPQPSSSSAYWWGAIIAFALQ
                     IYCDFAGYSNIARGLARWMGYDFPDNFNHPYISVSLREFWTRWHISLSTWFRDYLYIP
                     LGGNRKGKWRSHLNMAVTMVISGLWHGAAWTYVTWGALHAFFLSLERITGWNTFVSKR
                     KALKVLAWFFTLIQVLVAWVFFRAESIEQAWQIIKTMFAFTGEFDFGWGLNGWVFV"
BASE COUNT          386 a          315 c          370 g          555 t
ORIGIN      
        1 ggcgaagatt gtactgaaca tttctattca tcggcattat acatgaatag gttcgactga
       61 tttagaattg catctggtat aatattcgtg cattcgtggc cattctataa aaaaaggctg
      121 ttggattttt ctttccttcg agttgaggct aaataaagat tataaagtgt caaccccaat
      181 tatgaactga tccaattttt gtgaacgaaa gactcctctg tacactcacc acaggcacac
      241 gctgaaaagc accacccttg atatcaaacc cggccagctg tggctttgca attttgtatg
      301 ggctgtaaat ctcaggttaa tagaatccat ccatagcagc tttgcataaa aaattctatt
      361 tttatcggcc taaacagaac catgcttttt aattcgctca tatttgtagc ttttgccatt
      421 gtttattttg tcgtatcgaa attcataggt aaaagggtga aagctaggtt attctggcta
      481 acgggaatgt cgtttttttt ctacggatgg tgggactggc gttttctttt tctgatcatt
      541 tttagcggat tgatcgatta ttatgctgca ctggccatgg ttcgttggga aaagtaccgg
      601 aaattactgc ttttcctttc gatggctgga aatatcggct ctctctcggt atttaaatat
      661 tcgggattta ttgcccaaaa catcgacatg ctgtttggtc ttaccggagc attttcgctt
      721 gcaggtaata ttccaccctt cttccttgta acccctgtcg gaattagctt ttatactttt
      781 caaagcatga gttatactct cgatgtttat catcgaaaat ttacgccgac aagggatgtt
      841 atccatttct ttgctttttt atctatgttt cctcagctcg ttgccggccc aattattcgt
      901 gcaaaagaaa tgttgccgca acttcttgtt gtgaagaagg ttgaaagcga tgaaaaatgg
      961 gctggttttc gctacgtggt gaaagggtat tttatgaaaa tggtgattgc cgacaattta
     1021 gctcctttgg tagtaactgc gttcagcgcc ccgcagccca gcagttcttc ggcatattgg
     1081 tggggagcca ttattgcttt tgccttgcag atttattgcg attttgcagg ctatagcaat
     1141 attgcgcgcg gccttgccag atggatgggc tacgactttc ccgacaattt caatcatccc
     1201 tatatatcgg tttctcttcg cgaattttgg acccgatggc atatttcact ctctacatgg
     1261 tttcgtgact atctttatat tccgctggga ggaaaccgga agggaaaatg gcggtcgcac
     1321 cttaacatgg ctgtgactat ggttatttct ggtctttggc atggggcagc gtggacgtat
     1381 gtaacatggg gggcacttca tgcttttttc ctttctttgg agagaataac aggttggaat
     1441 acattcgttt cgaagagaaa agccttgaag gttcttgcct ggttttttac gttaattcag
     1501 gtgctggtag cgtgggtatt ctttagggcc gaaagcattg agcaggcttg gcagattatt
     1561 aaaaccatgt ttgcgttcac tggagaattt gactttggct ggggccttaa tggttgggtt
     1621 tttgtt
//
LOCUS       DAOPUW010000504         1625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_84557_length_1625_cov_2.471975, whole genome shotgun sequence.
ACCESSION   DAOPUW010000504 DAOPUW010000000
VERSION     DAOPUW010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84557_length_1625_cov_2.471975"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PKJ43_08135"
     CDS             complement(<1..759)
                     /locus_tag="PKJ43_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72576.1"
                     /translation="MRKLILLLLSVVLFSGCEKMAEYYFGFNKQPNISYDDFLPGLNV
                     FGVLSYGPSLDSVNHHFEVQRMMFVRENIDSSEISDAKIQLVRKTLQNEVFEYTLDTI
                     DAGIYANPEIVTAPGDEWSFRCTYDTFSVVSQCVIPNVPVVTSLKITNNHNLNVVLAA
                     DTSSYLYYLYLVGNEDVVASKIIPQRGYVTETALLADWDLSNSSSVLFIFACDKNLET
                     YYTTSNTFFKPNAFREPYTTVDGGYGTFGAMSSVL"
     gene            complement(765..>1625)
                     /locus_tag="PKJ43_08140"
     CDS             complement(765..>1625)
                     /locus_tag="PKJ43_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72577.1"
                     /translation="PKNLKVQAAWGKYAQYIVSMNTQEFEFNQLLDYYYPLINREPGL
                     SFHYIVGAEKKILRDQTLSIDLYYKDIARTYTFDMLQSQYETFALSEKIIEGRGRSYG
                     VELLWKGSYKLFSGWLGYTYSRSTRSFPHILNGQEYDYDYERRHSVKAAINFQATPRI
                     AYSASFIAQSGVPRSMETTYQTHYVYDPLTGNMLHSMQYVNGLKNNVRMPWLFYLDLG
                     LQKRLVGGFGREIAEFFGANESYLTVNVYNVLFFRRNILYYYPMGYDLFFPISDNYLP
                     VVAAGYTIKF"
BASE COUNT          542 a          348 c          303 g          432 t
ORIGIN      
        1 aagaacgctg ctcatagcgc caaaagtacc ataacctcca tcaacagtgg tgtatggttc
       61 cctaaaagca ttaggtttaa aaaaagtgtt cgatgtggtg taataggttt caagattttt
      121 atcacacgca aaaatgaata gcaccgatga agagtttgac aaatcccaat ccgcaagtaa
      181 cgcagtttca gtaacgtaac cccgctgagg aattatctta gacgcaacaa catcctcatt
      241 accaacaaga tataagtagt ataaatacga agaagtatca gcagcaagta caacattgag
      301 attgtggtta ttggtgattt tcagcgaagt taccaccggc acattgggta tgacgcactg
      361 agaaacaact gaaaaagtat cgtatgtgca gcgaaacgac cattcatcac ctggagcggt
      421 aacaatttca gggttggcat atattccagc atcaattgtg tcgagggtat attcaaaaac
      481 ttcattttgc aaagtttttc gcactaactg aattttggcg tcagagattt ccgacgaatc
      541 aatgttttcg cgaacaaaca tcatccgctg aacttcaaaa tgatgattta ctgaatcgag
      601 agaaggacca tagcttaaca ccccaaagac attcagcccc ggcaaaaaat cgtcatacga
      661 aatattgggt tgcttgttga atccaaagta gtattcggcc atcttttcac agccagaaaa
      721 aagaacaaca ctaagaagca ataggatcag ttttctcatc acgatcagaa ttttatggta
      781 taacccgccg caacaaccgg caaataatta tcagaaatag gaaaaaacaa atcataaccc
      841 atgggataat aatacaggat atttcttctg aagaacaata cattatacac attcaccgta
      901 agatacgatt cgttggctcc gaagaattca gcaatttcgc gaccaaagcc accaacaagc
      961 cgtttctgaa ggcctaaatc gagataaaag agccagggca ttcgcacatt attcttcaag
     1021 ccattgacat actgcatact atgcagcata tttcccgtaa gcggatcata cacataatga
     1081 gtttgatagg ttgtttccat agaacgggga accccacttt gcgcaataaa cgacgcacta
     1141 taggctattc gtggtgttgc ctgaaagttg atggccgctt taaccgaatg tctgcgctcg
     1201 taatcatagt catattcctg tccgttcaaa atgtgaggaa acgaacgagt cgaccgcgaa
     1261 taagtatagc ccagccaacc cgaaaacaac ttataactgc ctttccacaa gagctctacc
     1321 ccatacgaac gacctctgcc ttcgataatt ttttcggata atgcaaaagt ctcatactga
     1381 ctttgcagca tatcaaatgt gtaagttctt gctatatcct tataatacaa atcgatggaa
     1441 agtgtctgat ctcttaatat ttttttctcg gcacctacaa tataatgaaa ggaaagtccc
     1501 ggctcgcgat ttataagtgg ataataataa tcgagcaatt ggttaaactc aaattcctgt
     1561 gtattcatgc tcacaatata ttgggcatat tttccccatg cagcttgaac cttaagattt
     1621 ttagg
//
LOCUS       DAOPUW010000505         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_85275_length_1616_cov_4.062780, whole genome shotgun sequence.
ACCESSION   DAOPUW010000505 DAOPUW010000000
VERSION     DAOPUW010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85275_length_1616_cov_4.062780"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PKJ43_08145"
     CDS             <1..492
                     /locus_tag="PKJ43_08145"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNZ72578.1"
                     /translation="PEINKGLEKMDKVLISSFEDLESYVGKELGVSEAHEITQEQINR
                     FAAATLDFQWIHTDPERAKTESPFENTIAHGYLTLSLLPYLWNQIVEVQNLKLMVNYG
                     IEKFKFNQAVVVGSKVRLRAKVVSVVNLRGTVKAQIKVTLEIEGSKKSAYEGDVVFLY
                     HFN"
     gene            complement(520..1143)
                     /gene="folE"
                     /locus_tag="PKJ43_08150"
     CDS             complement(520..1143)
                     /gene="folE"
                     /locus_tag="PKJ43_08150"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNZ72579.1"
                     /translation="MEFSLNDQNGNYQKIEKYDSEVTGKLAENYSSILQILGEDVTRE
                     GLQKTPIRVAKAMQFLLQGYKMDPEEILRSAMFNEDYKQMVVVKDIELYSMCEHHMLP
                     FIGKAHVAYIPNGYITGLSKIARVVDAFSRRLQVQERLTTQIKDCINDTLHPLGVAVV
                     IEAQHLCMQMRGVQKQHSVTTTSDFVGAFTRDATRNEFMRIISSSMH"
     gene            complement(1437..>1616)
                     /locus_tag="PKJ43_08155"
     CDS             complement(1437..>1616)
                     /locus_tag="PKJ43_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose/galactose MFS transporter"
                     /protein_id="HNZ72580.1"
                     /translation="RYTKIGSSFLIMAIAGGAIIPVIYGFLADSFSPRGAYMIALPAY
                     LFILAYALFYKKIKP"
BASE COUNT          517 a          326 c          317 g          456 t
ORIGIN      
        1 ccggaaatta ataaaggatt ggaaaaaatg gataaagtat taattagcag tttcgaagac
       61 ctcgaaagtt acgttggaaa agaattagga gtttctgaag ctcatgaaat aacccaggaa
      121 caaatcaacc gttttgccgc ggcgacactc gattttcaat ggattcatac cgaccccgaa
      181 cgcgcaaaaa cagagtctcc gtttgagaat actattgctc atggatattt aaccctctcg
      241 ctgttacctt atctctggaa ccaaatagtt gaggtacaga atctaaaatt gatggtaaac
      301 tatggcatcg aaaaatttaa gtttaaccaa gcggtagttg taggcagtaa agttaggctt
      361 cgcgccaagg ttgtgtcagt tgtaaacctt cgcggaactg taaaagctca gataaaagtt
      421 accctggaaa ttgaaggcag caagaaatct gcttacgaag gtgatgttgt gtttctttac
      481 catttcaact aaggcaagaa aatataactg ggtgtgaaat tagtgcatcg aactgctaat
      541 gatgcgcatg aattcgttac gggttgcatc gcgggtaaaa gcgccaacaa aatcggatgt
      601 tgtggtaacc gagtgttgtt tttgaacgcc cctcatttgc atacaaagat gctgtgcctc
      661 aataacaaca gctacgccca acggatgcaa tgtatcgtta atgcagtctt tgatctgtgt
      721 agttaatctt tcctgcacct gtaaccggcg tgaaaaagca tcgacgaccc gggcaatttt
      781 actaagacct gtaatgtacc catttgggat ataggcaaca tgggctttcc cgataaaagg
      841 aagcatgtgg tgttcgcaca tcgaatataa ttcaatgtct ttcaccacaa ccatttgctt
      901 gtagtcttca ttaaacatag ccgaccgaag aatttcttcc gggtccattt tataaccctg
      961 taaaagaaat tgcatcgctt tggctacacg tatcggcgtt ttctgcagtc cctcgcgggt
     1021 aacatcttcg cccaatatct gtaagatact cgaataattc tcagccaact taccggttac
     1081 ttcagaatca tacttttcta tcttctgata attgccattc tggtcgttca atgaaaattc
     1141 catggtctca tttttcgtat tacataatca tcgcaaggcg tacagaactc acccatagcg
     1201 agggtttaaa ggtattgatt atttttctcc gacaccaaaa gaataccagg caaaatacaa
     1261 agtgcaatga aattatcatt tttacaaaac aataaccgca catcgatttc ttttaccaga
     1321 tattacaaag gagattttga atacgaataa catctggttt acgcccaatt tctggactgg
     1381 aaatagtaaa ctaagatttg aataaattaa tttcaaaaag gcttcgccct aacaaattac
     1441 ggcttgattt ttttgtaaaa gagtgcatat gccaggataa acagataagc cggcagtgca
     1501 atcatatagg ctccccgagg tgaaaaactg tcggctaaaa atccgtaaat aaccggaatt
     1561 attgcacctc cggcaatagc cataattaaa aaagaagatc caatttttgt atagcg
//
LOCUS       DAOPUW010000506         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_85305_length_1616_cov_2.640615, whole genome shotgun sequence.
ACCESSION   DAOPUW010000506 DAOPUW010000000
VERSION     DAOPUW010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85305_length_1616_cov_2.640615"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="PKJ43_08160"
     CDS             <1..961
                     /locus_tag="PKJ43_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72581.1"
                     /translation="SSGEKELYKKIAVLSKYYPENAGYILNAYNTSNNLVEALRMAEA
                     AEIHLKENSSYLNDVAEIQSMTDSGNGLFNTGSIYEWMNISEWRDFKLAVAKDRALID
                     SAANLAGVEPRLIVSVLVGEQIRLFNSNREAYKRWISPLKVLFVESSFSYGVTGIKLP
                     TAKQIETYLKDSSSVFYLGKEYEKLLDFETGAIATERLTRLTSTKNHFYSYLYAALNI
                     KQMKVQWERMGYPIDHRPEILATLYNIGFDSSQPKANPVVGGSGININGKRYSFGAIA
                     YEFYYSGELFDLFPFKKTRFDFNGPDTYALNPSSTTEAVDGGA"
     gene            1004..>1616
                     /locus_tag="PKJ43_08165"
     CDS             1004..>1616
                     /locus_tag="PKJ43_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72582.1"
                     /translation="MKTTRICRFQVDVAKAVLFFLFLLPAYSGLAQQSAIVLNWLPVD
                     EGVSFCEIDAPEKSVVNDSKLTLVRIDPSHCKFGFYTATQYENTPRSASSWADKFDLS
                     VVVNAGMYKSRNNNQNKGFLKVFNHLNNSTVSSSYNAMLAANPLDSSALSFRIFDMTC
                     DDFSTFDKKYHSFCQVMRMMDCKGQGMAFRKRPDQSCSMVLVST"
BASE COUNT          453 a          294 c          360 g          509 t
ORIGIN      
        1 ttcgagtggc gaaaaggaac tgtataaaaa gattgctgtt ttgagcaaat attatcccga
       61 aaacgctgga tacatattga atgcctataa tacatcgaat aatttggttg aagcactgcg
      121 catggcagaa gcggcagaaa tccatttaaa agaaaattct tcgtatttga atgatgtggc
      181 agaaattcag tcgatgacag attccgggaa tggacttttc aacaccggca gcatatatga
      241 atggatgaat atttcggaat ggcgcgattt taaactcgct gttgccaaag accgtgcgtt
      301 aatcgattcg gcagccaacc tcgctggagt agagccccgg cttattgttt ctgtgcttgt
      361 cggcgagcaa atacggcttt tcaactcgaa ccgtgaagcc tacaagcgtt ggataagccc
      421 attgaaagtg ttgtttgttg aatcgtcatt ttcgtatggc gttactggaa ttaaacttcc
      481 aacggccaaa cagatagaga catacctgaa agattcttct tctgtttttt atttgggaaa
      541 ggaatatgaa aaactgcttg attttgaaac tggagctatt gcaaccgaac gattaacccg
      601 cttaacatca acaaaaaatc atttctattc atatttgtat gcagcattga atattaaaca
      661 gatgaaagtg caatgggagc gtatgggtta tcctatcgat cataggcccg aaatattggc
      721 taccctttac aatatagggt ttgattcgtc gcagcctaaa gccaaccccg ttgtgggcgg
      781 ctctggaatc aatataaacg gaaaaaggta ttcgtttggc gcaattgctt atgagttcta
      841 ttattctggg gaacttttcg atttgttccc tttcaaaaaa actagattcg actttaacgg
      901 ccctgatact tatgcattga atccttcttc tactaccgag gcagtggatg gtggtgctta
      961 gtctttttgc tacttggtgt aattatttta tgttacaatt tatatgaaaa caacgcgaat
     1021 ttgcagattt caagttgatg tggccaaggc tgtgttgttt tttttatttc tgttgccggc
     1081 ttattcgggt ttggcacagc aatctgctat cgttttaaat tggttacctg ttgatgaagg
     1141 ggtttctttt tgtgaaattg atgcaccaga aaaatcggtg gtaaacgatt cgaaactaac
     1201 gttggtcaga attgatcctt cgcactgtaa atttgggttc tacactgcaa cccaatatga
     1261 aaatactcca cgcagcgctt cttcgtgggc cgataagttt gatctgtcgg ttgtggtaaa
     1321 tgcaggaatg tataaatcga ggaacaacaa tcaaaataaa ggttttctga aagtttttaa
     1381 tcacttgaac aatagtactg taagttcaag ctataatgcc atgctggctg ccaatccgct
     1441 cgattcatct gcattgtcgt tccgtatttt tgatatgact tgtgatgatt tttcgacatt
     1501 cgataaaaaa taccattcgt tttgtcaggt tatgcgcatg atggattgta agggacaggg
     1561 catggcattt cggaaacggc ctgatcagtc gtgcagcatg gtattggttt cgaccg
//
LOCUS       DAOPUW010000507         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_85327_length_1616_cov_2.117873, whole genome shotgun sequence.
ACCESSION   DAOPUW010000507 DAOPUW010000000
VERSION     DAOPUW010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85327_length_1616_cov_2.117873"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1616
                     /locus_tag="PKJ43_08170"
     CDS             <1..>1616
                     /locus_tag="PKJ43_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72583.1"
                     /translation="NFQVVRTEANLWGRWFNPIPEKVISTGETVSKDDGSFSVSFVAM
                     SNDIDNLDRIIQYTINADATDGNGESTHNEFSLRISKSNLIINCNLPEVVHNDNFSGT
                     TVNTTNLNEKEVASTVSISISRLKAPEGFIHNRRWTAPDTFLLDEKTFKNKFPSYPYR
                     EEQLPSNWKTDKVVFNQSVKLPNSDKLPLTEWRNSEPGYYRVDIEALSADGKQKTKWF
                     RTVRLIAQKPSPAQCTSDWVSSIKSKGEPGEVAEIWITAMNAESPVRYELVKEKEIIA
                     KEIIYPGKKAYRLQIPIDETYRGNVTAQFTHVANNRNYTKQVEIIVPHTNKMIDISFS
                     SFRDELLPGEPEKWALKLTDKTGKGVAAEMVAALYDASLEQFAQHSWPTNFLQNNYTH
                     YYTWNTGIVAPTAKQLTYSQNIKGLLFWQKKYEMLQLLDYNAQYNYPAIKKERVMIRG
                     ISTQNYESEEIFFASSSKLEKNFDVAYEATGAVAEISVADAIDLDISEEGEKEDLASG
                     QQSEKPDFSSVNVRTNFNETAFFYPNLYSD"
BASE COUNT          559 a          301 c          320 g          436 t
ORIGIN      
        1 gaattttcag gttgtacgga ctgaagcaaa tctttggggg cgctggttta acccaattcc
       61 cgaaaaagta atatccacag gagaaacagt aagtaaagac gatggtagtt tttctgtttc
      121 atttgtcgcc atgagcaacg atatcgataa tttagaccga attatacaat acacaataaa
      181 tgccgatgca accgatggaa acggagagag tacacacaat gaattctctt tgcgtatcag
      241 caaatcaaac ctgataatta attgtaattt acccgaggtg gttcataacg ataattttag
      301 cgggacaacg gtaaatacaa ctaatcttaa tgaaaaagaa gttgcttcta cggttagtat
      361 ttcaatctca cggttaaaag ctcctgaggg ttttatacat aacaggcgat ggactgcccc
      421 ggatactttt ctgttagatg aaaaaacatt taaaaataaa tttccatcat atccatatcg
      481 tgaagaacaa ttgccctcga attggaaaac agataaagtt gttttcaatc aatcggttaa
      541 attgccaaat tccgataaat tgccattgac ggaatggcga aactccgagc ccggatatta
      601 tcgtgtcgac attgaagcct tgtcggccga tggaaagcaa aaaacaaaat ggttcagaac
      661 ggtaaggctc atagcacaaa aaccttcacc agctcaatgt acttctgatt gggtaagctc
      721 gataaaatcg aagggcgaac cgggcgaagt cgctgagatt tggattacag ccatgaatgc
      781 tgaatcacca gtgcgttatg aactggtaaa agaaaaagaa atcattgcca aagaaataat
      841 atatccaggc aaaaaagcgt accggcttca aattcctatt gatgaaacat atagaggtaa
      901 tgttacggca cagtttaccc acgttgccaa taaccgtaat tatacaaagc aggttgaaat
      961 aattgttccg cataccaata aaatgatcga catcagcttt tcatcattcc gtgacgaact
     1021 gctacctggt gagccagaaa aatgggcgtt gaaacttacc gataaaacag gtaaaggtgt
     1081 tgctgctgaa atggttgctg cattgtatga tgcttctctt gagcaatttg cacaacactc
     1141 atggccgact aattttttac aaaataacta cacccattac tacacctgga atactggaat
     1201 tgtagcccca acagcaaaac aacttactta ttcacaaaat attaaaggat tgttattttg
     1261 gcaaaaaaag tacgaaatgc ttcaattgct cgattataat gctcaatata actatccggc
     1321 aatcaagaaa gaacgagtca tgattagggg aatcagtacc caaaattacg aatctgagga
     1381 aatatttttt gcttcctcct ccaaacttga aaagaatttt gatgtggctt acgaagcgac
     1441 aggagctgtt gcagaaatta gtgttgctga tgctatcgat ttagatataa gcgaagaagg
     1501 tgagaaagaa gatttagctt cgggtcaaca aagcgaaaaa cctgattttt cgtcagttaa
     1561 tgttcgtaca aattttaacg aaacggcgtt tttctatccc aatctgtata gcgata
//
LOCUS       DAOPUW010000508         1615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_85414_length_1615_cov_2.078846, whole genome shotgun sequence.
ACCESSION   DAOPUW010000508 DAOPUW010000000
VERSION     DAOPUW010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85414_length_1615_cov_2.078846"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PKJ43_08175"
     CDS             complement(<1..511)
                     /locus_tag="PKJ43_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNZ72584.1"
                     /translation="MIIDSLFWIVPVASLLALGFAWWFFAQMKKADEGTDTMKRIAQY
                     VRQGAMSYLKQQYKVVGIVFIVLSLLFVLMAYGFDFQNGWTPFAFLTGGIFSGLAGFI
                     GMRTATYASARTANAARHSLNSGLKIAFRSGAVMGLVVVGLAVLDIALWYLILNYFID
                     EPKTTHKLII"
     gene            complement(747..>1615)
                     /gene="prfB"
                     /locus_tag="PKJ43_08180"
     CDS             complement(747..>1615)
                     /gene="prfB"
                     /locus_tag="PKJ43_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_154199409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNZ72585.1"
                     /translation="LKVFFDFWKEGEGSADDVEKQYGLALSKIEDVEMMNMLRSEEDH
                     LGAILRINSGAGGLDSMDWAEMLLRMYLRWGERNGYETKVVDILRDDEAGIKSATIEL
                     AGEMAYGYLKGENGVHRLVRLSPFNANAKRHTSFASVFVAPAIDESIQITINPADIKW
                     DTYRSSGAGGQNVNKVETGVRLTHIPTGIVVENTETSSQHNNRENAMRLLKSHLYELE
                     LRKKKEKQAEIEGKKLSIEWGSQIRNYILHPYKLVKDVRTNFETSNVQDVLDGELNDF
                     IKAFLMQYGGEY"
BASE COUNT          450 a          370 c          322 g          473 t
ORIGIN      
        1 taataataag tttatgggta gttttgggtt cgtcgatgaa atagtttaga ataaggtacc
       61 acaaagcaat gtctaacact gccagtccca ctaccacaag acccatgacg gctcccgaac
      121 ggaatgcaat cttgagccca ctattgagcg agtgccttgc agcattggcg gtgcgtgcag
      181 aggcataggt ggctgtccgc atgccaatga aaccggcaag ccctgaaaat atacctcccg
      241 tcagaaaggc aaatggagtc caaccgtttt gaaaatcaaa gccatatgcc attaatacga
      301 atagtagcga gagaacgata aatactattc ccacaacctt gtactgctgc tttaagtagg
      361 acatggcacc ttgtcgaaca tattgtgcaa tccttttcat ggtatcagta ccctcgtcag
      421 cttttttcat ttgtgcgaag aaccaccatg caaaaccaag tgccagaagc gatgcaaccg
      481 gaactatcca aaataaagag tcaataatca tttcaataga ttttattcag ggttagaaat
      541 tcatatccaa attaagtgtg tttcaatgca aacacaaata ttcactattt tttttattta
      601 gagttatatt tttttaaaaa acgctaaaat ttattgtaga acaaatcgaa aaacaggtca
      661 taaaataccc tatgtcaggt tatctaaaca gataataatt aaaaaagttt aagtttttta
      721 aatattgttg aaacaataaa tccttactaa tattcaccac cgtattgcat caggaaagct
      781 ttgatgaaat cgttgagttc gccgtcgagt acatcctgaa catttgatgt ttcgaaattg
      841 gttcggacat cctttaccaa tttgtatgga tgaagtatgt agttgcgtat ttgcgatccc
      901 cactcaatgc tcaatttttt cccttctatc tcggcctgtt tctccttttt ctttcgtagt
      961 tccaactcgt aaaggtgaga ttttaacagt ctcatggcat tctcacgatt attgtgttgc
     1021 gagctggttt cggtgttttc aaccacaatc ccggtgggaa tgtgggtcaa gcgcactccg
     1081 gtctccactt tatttacatt ttgccctcct gcccccgaag aacgataggt atcccacttt
     1141 atatcggcgg gatttatggt aatttgaatg gactcgtcaa tggcaggggc tacaaaaacc
     1201 gaggcgaacg atgtatgacg tttggcattg gcgttgaaag gcgagagcct caccaggcgg
     1261 tgaacgccat tttcaccttt gaggtatccg taagccattt cgccggctaa ctcaatggtg
     1321 gcagatttta tacccgcttc gtcgtcacga agtatatcaa ccactttggt ttcataacca
     1381 ttcctttcgc cccatcgtag gtacatccgc aggagcattt cggcccaatc catgctgtcg
     1441 aggccgccgg caccggagtt gattcgtagg atggcaccca ggtggtcctc ctcgcttcgg
     1501 agcatgttca tcatttccac atcctcaatt ttcgagaggg caagcccata ctgtttctcc
     1561 acgtcgtcag cgctcccttc accctctttc caaaaatcga agaatacttt taaat
//
LOCUS       DAOPUW010000509         1612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_85688_length_1612_cov_1.842004, whole genome shotgun sequence.
ACCESSION   DAOPUW010000509 DAOPUW010000000
VERSION     DAOPUW010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85688_length_1612_cov_1.842004"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /gene="sufD"
                     /locus_tag="PKJ43_08185"
     CDS             complement(<1..1288)
                     /gene="sufD"
                     /locus_tag="PKJ43_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNZ72586.1"
                     /translation="MAEVAQYKASKEIADLFYQNRQLYYDGCSPQMNEARELAIRKFQ
                     EKGIPSRKNEDYKYTDLREAFENDFSVVPSYVEQSVDLHDLFNCDVPLLDTHIVLLIN
                     GWYYGRNRRIGDLPDGVICCSLQHASKEHSDIFNTYYNAQAVGSEDPLANMNTALAKD
                     GLFFYIPDNVTIDKPVQVINLLFSKENTLAIQRNLFVCGKNSEATIVFCDHTLNHNYY
                     ITNNVSECVVGEDARFNFFSTQNQHNGTVNLTHLFANVGKQANLLTNTITLNGGLVRN
                     NFSVNLSGEYANASLNGLSLADKKQHVDNFILMNHESPKCISYQLFKNILDEQSSGAF
                     SGRIHVFRDAQKTEAYQQNNNVLMSADAEMNTKPQLIIDADDVRCSHGATVGRIDENA
                     LFYLRARGIGEREARLMIMFAFADDVISKINVGPLRE"
     gene            complement(1292..>1612)
                     /gene="sufC"
                     /locus_tag="PKJ43_08190"
     CDS             complement(1292..>1612)
                     /gene="sufC"
                     /locus_tag="PKJ43_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665521.1"
                     /note="part of SUF system involved in inserting
                     iron-sulfur clusters into proteins; in Escherichia coli
                     this protein forms a complex with SufBD; the SufBCD
                     complex stimulates the cysteine desulfurase SufS in
                     conjunction with SufE; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNZ72587.1"
                     /translation="GFSGGEKKRNEIFQMAMLEPKLAILDETDSGLDIDALRIVANGV
                     NKLKSPENATIVVTHYQRLLDYIVPDFVHVLYKGRIVKSAGKELAVELEEKGYDWIKK
                     ELGE"
BASE COUNT          452 a          358 c          322 g          480 t
ORIGIN      
        1 gctcacgaag cgggcctacg ttaattttag agattacgtc atcggcaaaa gcaaacataa
       61 tcatcaggcg tgcctcacgt tcacctattc cacgggcccg taaataaaac aatgcatttt
      121 catcgatgcg gccaacagta gctccatggc tgcaccgtac atcatcggca tcaattatta
      181 actgaggctt tgtgttcatc tcagcatcgg cactcatcag cacattattg ttctgctgat
      241 aggcttcggt tttctgtgca tcacggaaca catgaatacg ccctgaaaat gcaccgctcg
      301 actgttcatc caaaatgttc ttaaaaagct ggtaactaat acattttgga ctttcatgat
      361 tcataaggat gaaattgtcg acatgttgtt tcttatcggc caacgacaat ccgtttaagc
      421 ttgcattggc atactcgcca gacaggttta ccgaaaaatt attccttacc aatccgccat
      481 ttaaggtaat ggtattggta agaaggtttg cctgtttacc aacatttgca aacaaatggg
      541 taagatttac cgttccgtta tgctgatttt gggtcgagaa aaaattaaag cgggcatctt
      601 cgcccacaac acattcgctt acgttattgg taatgtaata gttatgattc aatgtgtgat
      661 cgcaaaaaac aattgttgct tcactgttct ttccacaaac aaacaaattc cgttgaatgg
      721 ccaatgtatt ttctttggaa aacaacaaat taataacctg aactggtttg tcgatagtga
      781 cattatcggg gatatagaaa aacaacccgt cttttgccag cgcggtattc atattggcca
      841 ggggatcttc gcttcccaca gcctgagcgt tataataggt attaaaaata tcagaatgct
      901 ctttcgatgc gtgttgaagg ctgcaacaaa ttaccccatc aggcaaatca ccaatgcgac
      961 gattacgtcc gtagtaccaa ccgttgatca acaatacgat atgggtatcg agcagaggta
     1021 catcacaatt aaaaaggtcg tgcagatcaa cagattgctc tacatacgaa gggactacag
     1081 aaaagtcgtt ttcgaaagct tcccgaaggt cggtatactt atagtcttcg tttttccgtg
     1141 aagggattcc tttctcctga aattttcgga tagccagttc acgagcctca ttcatctgcg
     1201 gcgaacatcc gtcgtaatac aactgcctgt tctggtaaaa tagatcggca atttctttag
     1261 atgctttata ttgagctact tcagccatga tttattctcc caactctttt ttgatccaat
     1321 cgtagccttt ttcttcaagt tcgacagcaa gttcttttcc ggccgactta acaattctcc
     1381 ctttgtaaag cacatgtaca aaatcgggga caatataatc gagcaggcgt tggtaatggg
     1441 taacaacgat ggttgcattt tcgggcgatt ttagtttgtt taccccattg gcaacaatac
     1501 ggagggcatc aatatcgagc ccggaatcgg tttcatcgag gatggcaagc tttggttcga
     1561 gcatggccat ctggaatatt tcgtttcgtt tcttttctcc tcctgaaaaa cc
//
LOCUS       DAOPUW010000510         1612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_85694_length_1612_cov_1.652537, whole genome shotgun sequence.
ACCESSION   DAOPUW010000510 DAOPUW010000000
VERSION     DAOPUW010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85694_length_1612_cov_1.652537"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PKJ43_08195"
     CDS             complement(<1..732)
                     /locus_tag="PKJ43_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNZ72588.1"
                     /translation="MKNIKNSIFYLLVTGGFSALIYWILSQGKFLEAGRSIVTVNSNK
                     TQWSEFLASLMHNVQHPLAILLAQIVTIIIVARFFGWMFRKIGQPSVIGEIIAGIVLG
                     PSLLGMYFPEFSNALLPVASLGNLQFLSQIGLILFMFVIGMELDLKVLKNKANEALVI
                     SHASIIIPFALGIGLSYFIYYRFAPAGVEFLSFSLFMGIAMSITAFPVLARIVQERGI
                     HKTKIGTIVITCAATDDITAWCILAA"
     gene            928..>1612
                     /locus_tag="PKJ43_08200"
     CDS             928..>1612
                     /locus_tag="PKJ43_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72589.1"
                     /translation="MKRSIQVGFWERIARIILKNRIAILTSIVALTIFLAFQWRNLGM
                     TYTEANLLPQKHIVNRQYSDFLDKFGEEGNLIVIGFQDTALFTPKAYAAWNELMTGLK
                     NAKAEVELVVSLNGLKKLEKDTLAQQFRMIPLIDQNQTNDAAYLQKIKTELFERLPFY
                     EGLLFNKASGSVRSVVYMRKSVVNTAGRKTFIVEKLVPAIDKFEKETGIDLKVSGMPY
                     IRTINAENMK"
BASE COUNT          534 a          373 c          323 g          382 t
ORIGIN      
        1 ggccgccaaa atacaccacg cagtaatatc atcagtggca gcacaagtga tgactatggt
       61 tccgattttg gttttatgaa tgccacgttc ttgaacgatt cgggctaaaa ccggaaatgc
      121 agtgatgctc atcgcaatac ccataaaaag gctgaacgac aaaaattcga cacctgcggg
      181 agcaaaacga taatagatga aatatgacaa accgataccc aatgcaaacg gaataatgat
      241 gctggcatga ctaattacta aagcttcatt ggccttattt ttcaggactt taagatcgag
      301 ttccatgcca ataacgaaca tgaaaagaat caaaccgatt tgacttagaa actgaagatt
      361 tcccaacgat gctaccggta ataacgcatt tgaaaactct ggaaaataca tacccaacaa
      421 cgacgggccg agtacaattc cggcgataat ttcacctatc acgctcggct gaccaatctt
      481 tcggaacatc catccaaaga aacgggcgac aataatgatg gtgacaatct gagccaaaag
      541 tatcgctaac ggatgttgca cattgtgcat cagcgaagct aaaaattccg accattgggt
      601 tttattcgaa ttgaccgtca cgatggatct tccggcttct agaaattttc cttgcgagag
      661 aatccaataa attagtgccg aaaaacctcc ggtaactaag agataaaaaa tggaattttt
      721 aatattcttc ataaacaaag gcgtgctttt ttttactttc aatcactcgc aaagatgcga
      781 aaccaaatat ataaattata acggcaaaaa aaatagtaac ccaaatttaa tgtgccttaa
      841 aaaaacctgg ccaaaccgca ataaaattgg actgcttcat ccaaatttcc ccttggtttc
      901 cttacctttg caaccatttc taaaaatatg aaacgcagca tacaagtagg gttttgggaa
      961 cggattgccc gtatcatact gaaaaacagg atcgcaatcc taacctcgat tgttgccctt
     1021 accattttcc tggcttttca gtggcgaaat ctgggcatga cctacaccga ggccaacctg
     1081 ttgccacaaa aacacatcgt caaccggcaa tacagtgact ttctggataa attcggcgaa
     1141 gaaggcaacc tcattgtaat tgggttccag gacaccgcgc tttttacacc caaagcttac
     1201 gccgcctgga acgaactcat gaccgggctc aaaaatgcta aagccgaagt cgagttggtg
     1261 gtttcgctca acggcctcaa aaaactcgaa aaggacacgc ttgcacagca attcaggatg
     1321 ataccgctca ttgatcaaaa ccaaaccaac gatgcggcct acctgcaaaa gataaagaca
     1381 gaactgtttg agagactgcc cttttacgaa gggctgttat tcaacaaagc gtctggcagc
     1441 gtgcgatcgg tggtttacat gcgcaaaagc gtggtcaata cggctgggcg taaaaccttt
     1501 atcgtcgaaa aactcgttcc ggcaatcgac aaattcgaaa aagaaaccgg gatcgacctc
     1561 aaggtttcgg gcatgcctta catcaggacc atcaatgccg agaatatgaa ag
//
LOCUS       DAOPUW010000511         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_85722_length_1611_cov_3.384319, whole genome shotgun sequence.
ACCESSION   DAOPUW010000511 DAOPUW010000000
VERSION     DAOPUW010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85722_length_1611_cov_3.384319"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PKJ43_08205"
     CDS             <1..274
                     /locus_tag="PKJ43_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNZ72590.1"
                     /translation="IGTKRELIVGKELEIRKWLSDSHLYSSAFEVEKVIDDTTTVPHQ
                     FILHGSGWGHGVGMCQIGAAVMSRKGYSFEAILNHYFTGAKLEKIY"
     gene            complement(373..>1611)
                     /locus_tag="PKJ43_08210"
     CDS             complement(373..>1611)
                     /locus_tag="PKJ43_08210"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNZ72591.1"
                     /translation="VNGIKGEYFNNRNLEGEPVLTRVERKIDMVWDGSGIDQKVNSHD
                     ISVRWTAKLIPGVSGKYIISFTGDDGYRVWLDNELIMDAWRNQGPTENYKAIELFARR
                     EYDLKIEYFQGEGGAVAQLKWARIIEGQTDAYLKEVAKCDVIVYVGGLSAALEGEEMP
                     VSFDGFDGGDRTKIDLPAVQTNTLKKLKKLGKPIILVNMSGSALALNWENENIDAILQ
                     AWYPGQQGGTAIADVLFGDYNPAGRLPVTFYKSVNDLPDFSSYDMEGRTYRYFKGEAL
                     YPFGFGLSYTTFSYSNLQLPENASTSDKIRVSVDVENTGKRDGDEVVQLYVKIEDAAV
                     PVPLHALQAFKRINLKTGEKKTVTFELEPKQFAVIDDENKRVVQPGNFTIFVGGQQPG
                     KLDKGLNKSLTLSGKQFYIE"
BASE COUNT          411 a          355 c          351 g          494 t
ORIGIN      
        1 tatagggaca aagcgtgaat tgattgttgg taaagaactt gagattagaa agtggttgtc
       61 agatagtcat ttatatagtt cagcgttcga ggtcgaaaaa gttattgacg atacgacgac
      121 agttcctcat cagttcattc tgcatggatc gggttggggg catggtgtgg gtatgtgcca
      181 aatcggagcg gcggtaatga gccgaaaagg atactcattt gaagccatct tgaatcatta
      241 ttttacagga gcaaagctcg aaaaaatata ttgacaaatc ttgcatgtgc ttctcaacca
      301 gcatataatt caaattatac aaaaaaggcc ccgaatgaac ggagcctcct gagtatttaa
      361 tggtggattt tgttattcga tatagaactg ttttccgcta agtgttaacg atttatttag
      421 ccctttatcc aacttgccgg gttgttgtcc tccgacaaaa attgtaaaat ttccgggttg
      481 taccacgcgt ttgttttcat cgtcgatgac cgcaaattgc ttaggttcga gttcaaaggt
      541 aactgtcttt ttttcacctg tttttaggtt gattcgtttg aatgcttgca gcgcatggag
      601 tggaaccgga acagccgcat cttcaatttt tacatacaac tgaacaactt cgtcaccatc
      661 gcgtttgcca gtgttctcaa catcgaccga cactctgatt ttgtcagatg ttgaagcatt
      721 ttcgggcaat tgaaggttgc tgtatgagaa ggttgtgtaa ctaagtccga atccgaatgg
      781 gtaaagggcc tcacccttga aataccggta tgtgcggcct tccatgtcgt agctcgagaa
      841 gtcgggcagg tcgtttaccg atttatagaa tgtaacagga agccttcctg ctgggttata
      901 atcaccgaaa aggacatcgg caatggctgt cccgccttgt tgtcctgggt accatgcctg
      961 taatatggca tcgatgtttt cgttttccca attgagagcc agcgcacttc cgctcatatt
     1021 gacaagaata attggtttcc ccagtttctt aagttttttc aacgtgttgg tttgaacagc
     1081 tggtaaatca atttttgtac ggtcgcctcc gtcaaaccca tcaaaggaaa ccggcatttc
     1141 ttcgccttca agcgccgcag aaaggccacc aacataaaca attacatcgc atttggcaac
     1201 ctctttcaag tatgcatcgg tttgcccctc gattattcgt gcccatttca actgggccac
     1261 tgctccgcct tcgccctgaa agtattctat cttcagatcg tattccctac gagcaaatag
     1321 ttcaatggct ttgtagtttt ctgttggccc ctggtttctc catgcatcca taatgagttc
     1381 attatcgagc cataccctgt agccgtcgtc tccggtaaac gaaataatgt atttcccgct
     1441 aacaccggga atcagttttg cagtccaacg aactgaaata tcgtgagaat tcactttttg
     1501 atcaatgccg gagccatccc aaaccatatc gattttgcgt tctacacggg ttaatacagg
     1561 ttcgccttca aggttgcggt tgttgaagta ttctccttta attccgttaa c
//
LOCUS       DAOPUW010000512         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_85726_length_1611_cov_3.105398, whole genome shotgun sequence.
ACCESSION   DAOPUW010000512 DAOPUW010000000
VERSION     DAOPUW010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85726_length_1611_cov_3.105398"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PKJ43_08215"
     CDS             complement(<1..599)
                     /locus_tag="PKJ43_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72592.1"
                     /translation="MKRSGLQKFFKFLLLTIVGLFFLIGLGLSGAFLAVKFHLTDAPG
                     VVDINDRYFQEIKNKYGKHTKIEASSGEKELYKKIAVLSKYYPENAGYILNAYNTSNN
                     LVEALRMAEAAEIHLKENSSYLNDVAEIQSLTDSDDELFSTGSIYEWMNISEWRDFKL
                     AVAKDRALIDSVANLTGVEPRLIVSVLVGEQIRLFNSNR"
     gene            756..>1611
                     /locus_tag="PKJ43_08220"
     CDS             756..>1611
                     /locus_tag="PKJ43_08220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNZ72593.1"
                     /translation="MRLRLITIILLSVLSISVYSQSGKTEKIIESPENLEIEWVLDSI
                     HNSDSLNAAYPAQDLYKSYWSNTNIRYPSANFADKNDTIIFTLVSFGESQYVHPFKGK
                     VISKFGPRSGRMHTGTDVKLHSGDSVVCAFDGKVRLAKRFSGYGNMVLVRHDNGLETL
                     YAHLKTISVKVNDELKAGDLIGLGGRTGRATTDHLHFETRIFGDPFDPNKYIDFENFA
                     LRSNNLYYSKKKIFIEPDRIKVDPSIPEPVLAQRDSTKTKQANKNNSKDAVAQADVPT
                     KHVIQKGDN"
BASE COUNT          541 a          325 c          296 g          449 t
ORIGIN      
        1 tcacggttag agttgaaaag ccggatttgc tctccgacaa gcaccgaaac aataagccgg
       61 ggctccacac cggtaaggtt cgcaaccgaa tcgattaacg cacggtcttt ggccacagct
      121 agtttaaaat cgcgccattc cgaaatattc atccattcgt atatgctacc ggtactgaaa
      181 agttcgtcat cagaatcagt caatgactgt atttcagcga catcgttcaa ataagaagaa
      241 ttttctttta aatgaatttc tgccgcttcg gccatgcgca acgcttcaac caaattattc
      301 gatgtattgt aggcattcag aatatagcct gcattctctg gatagtattt gcttaggaca
      361 gcaatctttt tatacagttc cttttcgcca ctcgaagctt caatctttgt gtgcttcccg
      421 tatttattct taatctcctg aaaataacga tcattgatat cgacaacacc cggagcatcg
      481 gttaaatgga actttacagc caaaaaagcg cccgaaaggc ccaagccgat caaaaaaaat
      541 aaaccgacga ttgttaataa cagaaacttg aaaaactttt gtaaaccaga tcttttcatg
      601 taaaactgac tcttttaaaa accgcggcaa atgtataaaa agctggactg aattgttaaa
      661 aaatgcaatt actattcata aaatttcaaa tattattacg acaatgttat ctttgttagg
      721 aaaaaaaaga gggaatatta actaaagagc tgtaaatgag gttacgatta attactatta
      781 tactgctatc tgtgttgagt atttcagtgt attcgcaatc aggtaaaaca gaaaaaataa
      841 tagaatcgcc cgagaactta gaaattgaat gggtactcga ttcaattcat aattcagatt
      901 cattgaatgc tgcctatccg gcacaagatt tatataaaag ctattggagt aatactaata
      961 tcagatatcc atcggccaac tttgccgaca aaaacgatac aattattttc accttggtaa
     1021 gttttggtga atctcaatat gttcatccat tcaaaggaaa agtaatttcg aaatttggac
     1081 cacgcagtgg acgaatgcac accggcacgg atgttaagct tcattcgggc gattctgttg
     1141 tttgtgcttt cgatggaaaa gtacggcttg caaaacggtt tagcggatat gggaacatgg
     1201 ttctcgtgcg ccacgataac gggttagaaa cactttatgc tcaccttaaa accatctcgg
     1261 taaaagtaaa cgatgaacta aaagccggcg atttaattgg gctgggtggc cgtacaggaa
     1321 gagccacaac cgatcatttg catttcgaaa cacgtatttt cggcgatcca ttcgatccaa
     1381 acaaatatat tgatttcgaa aactttgcac tacgttcgaa taacttatac tattccaaaa
     1441 agaagatctt tattgaaccc gacagaatta aagttgatcc aagtatacca gaacctgttc
     1501 ttgctcaacg tgattcaaca aaaactaaac aagcgaacaa aaacaattca aaagatgctg
     1561 tggctcaggc cgatgttcct acaaaacatg ttatccagaa aggcgacaac c
//
LOCUS       DAOPUW010000513         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_85731_length_1611_cov_3.007069, whole genome shotgun sequence.
ACCESSION   DAOPUW010000513 DAOPUW010000000
VERSION     DAOPUW010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85731_length_1611_cov_3.007069"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="PKJ43_08225"
     CDS             <1..1093
                     /locus_tag="PKJ43_08225"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HNZ72594.1"
                     /translation="FNTKTKHVQQALLQMGTKTENISNPAFVQLPDSSFIAFFQQTHS
                     QNTILCHGKIVNKLLRWQSADTIYSNPGEKKITEEDHTELVPFLLSEEKNKLFLFKQT
                     NNSTVTLTTSNDKGKTWSNSKSIFSNKKKGSKNEFIVKYFSDGKNKIHLVFSDSSNPV
                     KPNNIYYAYYSNGAFYRVDGKKICTINQLPFDITKATCLYEAKKGKQAAFPADIVVDP
                     KGTPAVAYVKNNESNQASYHYNWYDGKKWKDNLVGMVGSKMERTSPINGINFSGGITL
                     SPSSEHTLYVSQKTGSAFEIEKRELNTAGNTWKTTVITSNSQSSNILPYTIKSVPGSE
                     GTILLWIMKSNYPEGNSFKSAIKLFTDKK"
     gene            1236..1610
                     /locus_tag="PKJ43_08230"
     CDS             1236..1610
                     /locus_tag="PKJ43_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72595.1"
                     /translation="MRRSILFISIFLSILAFSAKAQSEIPAFPGAEGGGTFTTGGRGG
                     EVYFVTSLEDTNTGDTATREGTLRWCLSRAGSKTIVFRVAGIIRLKSVLKITDNTTIA
                     GQTAPGEGICLAETRLRLKATT"
BASE COUNT          587 a          351 c          305 g          368 t
ORIGIN      
        1 tttcaataca aaaacaaagc atgtacaaca agcgttgctt cagatgggaa caaaaacaga
       61 aaatatcagc aacccagcct ttgttcaatt gcccgacagc agctttatag cattttttca
      121 acaaacacat agccaaaata caattctctg ccacggtaaa attgtaaaca aacttttgcg
      181 ctggcagagt gccgacacta tttattcaaa cccgggagaa aaaaaaatca cggaggaaga
      241 tcataccgaa ttagttcctt ttttgctttc cgaagaaaaa aacaaattat tcctgtttaa
      301 gcaaaccaac aattcaacgg tcacactaac aacaagcaac gacaagggca aaacatggtc
      361 caacagcaaa agtatcttta gcaacaaaaa aaagggaagc aagaatgaat tcattgtaaa
      421 atactttagc gatggaaaaa acaaaattca tttagtattc tccgacagca gcaatcctgt
      481 aaaacccaac aacatatatt atgcatatta ttcaaacgga gcattttaca gggttgacgg
      541 gaagaagata tgcaccatca accagcttcc attcgacata acgaaagcca cctgccttta
      601 cgaagccaaa aaaggcaaac aagctgcttt tccagccgat attgtagtcg acccaaaagg
      661 aacccctgct gtagcctatg taaaaaacaa tgaaagtaac caggcatctt accattataa
      721 ctggtacgat ggaaaaaaat ggaaagataa cttagtgggc atggttggga gtaaaatgga
      781 aagaaccagc cccatcaacg gaataaactt ctcaggggga ataaccctgt ccccaagttc
      841 agaacacacc ctgtatgttt cgcaaaaaac aggttcggcc ttcgaaatag aaaaaagaga
      901 attaaatacg gctggcaaca cctggaaaac aaccgttatt accagtaaca gtcaatcttc
      961 gaacatatta ccgtacacaa taaaaagcgt accaggcagc gaaggaacaa tattgctttg
     1021 gattatgaaa agtaactacc cggaaggcaa tagctttaaa tcggcgatca aattattcac
     1081 tgataaaaaa tgaatcatgt aaacggtatt ttttttcttt tatacgccac agagacaatt
     1141 tatcaccact tttacgtcag ttttagccct gcatagccta agcaaagtag taattttgaa
     1201 caggaaaccg ggcaagtgcc caaaataaaa atactatgcg cagatcaatc ttatttattt
     1261 caattttcct gtccattctt gctttttcgg ccaaagccca atcagagatc ccggcattcc
     1321 cgggagccga gggaggtgga acattcacga ccggaggacg gggcggagaa gtatattttg
     1381 taacctcact cgaagataca aatacaggcg acactgccac tcgtgaagga acattgcgct
     1441 ggtgcctgtc acgagcagga agtaaaacaa ttgtcttcag ggttgcagga atcatccggc
     1501 ttaaatctgt attaaagata accgacaata ccactatagc aggccaaact gcaccggggg
     1561 aaggcatttg ccttgccgaa acacggctca gattgaaggc aacaacataa t
//
LOCUS       DAOPUW010000514         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_85732_length_1611_cov_3.001928, whole genome shotgun sequence.
ACCESSION   DAOPUW010000514 DAOPUW010000000
VERSION     DAOPUW010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85732_length_1611_cov_3.001928"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PKJ43_08235"
     CDS             complement(<1..395)
                     /locus_tag="PKJ43_08235"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ72596.1"
                     /translation="MKQFAAFVKKEFRHILRDHWTMIILLVLPILMVILFGYGISTEV
                     KNTRFAVLDPSRDVVTQGIVNKLATSEYFILGDYLTSESQIEQCFTHSNYGLVLVFGN
                     RFGEQIIDPDGVQILLIADGSDPNTASILT"
     gene            complement(417..1193)
                     /locus_tag="PKJ43_08240"
     CDS             complement(417..1193)
                     /locus_tag="PKJ43_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ72597.1"
                     /translation="MTSIGTDKITQQIGKVIWAENLCKKFGAFTAVDHISFEIEKGEI
                     FGFLGANGAGKTTAMRMLTGLSKPTSGKGVVAGFDINTQSEQIKKNIGYMSQKFALYE
                     DLKVWENIRLYGGIYGVPKNEIAPRTDDLLHRLGFEKEKDTLVKSLPLGWKQKLAFSV
                     AIFHEPKIVFLDEPTGGVDPAARRNFWEMIYQAADRGITVFVTTHYMDEAEYCNRVSI
                     MVDGRIDALDTPKNLKDQFNASTMDEVFHHLARKSTRNAD"
     gene            complement(1186..>1611)
                     /locus_tag="PKJ43_08245"
     CDS             complement(1186..>1611)
                     /locus_tag="PKJ43_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ72598.1"
                     /translation="RKEFWEMLKRLKNEGITILVSTPYMDEAALCDRIALIQNGQFLK
                     IETPENIVNQFAQELWTVKGVQMSKLLNDLRQYEHIRTCFAFGDTHHITLESNMSTVE
                     NLQDYLQTKGHLQVEIEPTKASIEDCFMELTFEKSESYD"
BASE COUNT          457 a          360 c          312 g          482 t
ORIGIN      
        1 gttaatattg atgccgtgtt ggggtctgaa ccgtcggcaa taagcagtat ttgaacacca
       61 tcgggatcga taatttgctc accaaagcga tttccaaaca caagcaccaa tccataattg
      121 gaatgggtaa agcactgctc tatttgcgat tcgcttgtca aataatcgcc aagaataaaa
      181 tattcgctgg tggccaactt gttaacaatg ccctgggtaa caacatcgcg cgaagggtcg
      241 agcacggcaa aacgggtgtt tttcacttcg gttgaaatgc catatccaaa caaaataacc
      301 atcaaaattg gcaaaaccaa caagatgatc atagtccagt ggtcgcgcaa aatatggcgg
      361 aattcctttt ttacaaaagc agcaaattgc ttcataatac gcagttttat ttattcttaa
      421 tcggcgttac gggttgattt tcgtgccaaa tggtgaaata cttcgtccat tgtactggca
      481 ttaaattgat ctttaaggtt cttcggagta tcgagcgcat caatccggcc atcgaccata
      541 attgaaacgc ggttacaata ttcagcctcg tccatgtaat gagtggttac aaaaacggtg
      601 attccacggt cggcggcctg ataaatcatc tcccaaaaat tgcgccttgc ggcaggatca
      661 acgccaccgg taggttcgtc gaggaaaaca atttttggtt cgtgaaaaat ggctacagaa
      721 aaagcaagtt tctgtttcca tcccaatggc aacgatttca ccagcgtatc tttctccttt
      781 tcgaaaccaa ggcgatgcaa caaatcgtcg gtgcgaggtg caatttcatt cttcggaaca
      841 ccataaatac caccatacaa tcgaatattt tcccacactt tcaagtcttc gtaaagagca
      901 aatttctgac tcatgtagcc aatgtttttc ttaatttgtt ccgattgcgt attgatgtcg
      961 aaaccggcaa caaccccttt gcccgaagta ggtttgctta acccggtaag catacgcatg
     1021 gctgttgttt tacctgcacc gttcgcacca aggaaaccaa atatttcgcc tttttctatt
     1081 tcaaaagaga tgtggtcgac agctgtaaac gcgccaaatt ttttacatag attttcagcc
     1141 caaatcactt tcccgatttg ctgagtaatt ttatctgttc ctatactagt cataactttc
     1201 tgatttttcg aaagtaagtt ccataaaaca atcttcgatt gatgctttgg ttggttcaat
     1261 ttccacctga agatgccctt tcgtttgtaa ataatcctga aggttttcaa cagttgacat
     1321 attgctttcc agtgtgatat ggtgggtgtc gccaaaagca aagcatgttc taatatgctc
     1381 gtattgccgt aagtcgttca aaagcttcga catctgcaca cctttaacag tccaaagctc
     1441 ttgcgcaaat tgattcacaa tattttctgg agtttctatt ttcaaaaact gtccgttctg
     1501 gataagggca atgcggtcgc acaaagcggc ttcatccatg taaggtgttg aaaccaaaat
     1561 ggtgatccct tcgttcttca accttttcag catttcccaa aattctttac g
//
LOCUS       DAOPUW010000515         1608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_85990_length_1608_cov_2.611719, whole genome shotgun sequence.
ACCESSION   DAOPUW010000515 DAOPUW010000000
VERSION     DAOPUW010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85990_length_1608_cov_2.611719"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PKJ43_08250"
     CDS             complement(<1..495)
                     /locus_tag="PKJ43_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72599.1"
                     /translation="MRYISVALLSVLLFAQGCSYNQQPKTVVIDNQFSMLVPSFMKED
                     KEVRPGSPFQYANRFRNLYSTVFFDKKDAVKLPVEQYYNSQIQIIKNVLEKPMVTDSA
                     GITIDGQHAIHTEVFGKMQGEYIYYSHLLIEAPDKYYQVCVWTRGEDRKLKYANDIEA
                     LLYSF"
     gene            complement(526..1449)
                     /locus_tag="PKJ43_08255"
     CDS             complement(526..1449)
                     /locus_tag="PKJ43_08255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNZ72600.1"
                     /translation="MLLSIFKLYPTEQDPVAYAIPFFILLILIEAYVGYKQQHRNYLS
                     KDAAASIAMGIGSMLINFVMKSLAFGVYTIVYEFRLFEIGWHWWTWALIFLADDFTFY
                     WYHRFAHEIRILWAAHVNHHSSVEYSLSIALRQSWGELLFKYFFWLWLPVVGFPPMMV
                     FMMISISLIYQFWVHTELIHRFPKWFEFIMNTPSHHRVHHASNYRYLDRNHAGILIIW
                     DRMFGTFSEELDEEKPVFGITVNIHTFNPFKIASHEYIAIWHDILRAPTFKDKLNYIF
                     QAPGWSHDGQDLRAKVLRKTVANKQSTVAPN"
BASE COUNT          515 a          322 c          323 g          448 t
ORIGIN      
        1 gaacgaatag agtagtgcct caatatcatt agcatatttc aatttgcgat cttcacctcg
       61 tgtccaaaca catacttgat agtatttatc gggtgcttca atcaataaat gcgagtagta
      121 aatgtattcg ccctgcatct taccaaacac ttccgtgtga atagcatgct gcccatcgat
      181 agtaattccg gctgaatcag tcaccatagg cttttccaat acatttttga tgatttgaat
      241 ttgcgaatta tagtactgtt caacaggtaa ctttaccgca tcttttttat cgaagaaaac
      301 ggtagaatat aagtttctga accgattggc atattggaat ggggaacccg gtcgcacttc
      361 tttgtcttct ttcataaacg aaggaacaag catgctgaat tggttatcga tgaccactgt
      421 tttaggctgt tggttatacg aacaaccttg ggcaaaaagc aaaacagaaa gcaaagcaac
      481 agatatgtag cgcatcacat tttttttgtg caataatagg caaaactaat ttggtgcaac
      541 agtactttgt ttattggcaa ctgtttttcg caacactttt gcccgtaaat cctgcccatc
      601 gtggctccaa cccggtgctt gaaaaatgta attcagctta tctttgaagg taggagcacg
      661 gagtatgtcg tgccaaattg caatgtactc gtggctggca attttaaacg gattgaaggt
      721 gtgaatattc actgtgatgc cgaaaaccgg cttttcttca tctagttctt cggaaaatgt
      781 accaaacata cgatcccaaa taatgaggat accggcatgg tttctatcga ggtatcgata
      841 gtttgatgca tgatgaacac ggtggtgtga aggcgtattc ataataaatt caaaccattt
      901 tgggaacctg tgaatgagtt cagtgtgcac ccaaaattgg taaatcaaac taattgaaat
      961 catcataaac accatcattg gtgggaatcc cactaccggt aaccacaacc aaaagaagta
     1021 tttgaataac aactcgcccc aactttgacg aagcgctatc gacaaggaat actccacgga
     1081 tgagtgatga ttcacatgtg cagcccataa aatccgtatt tcgtgtgcga agcggtgata
     1141 ccaataaaac gtaaaatcat cggctaaaaa gataagcgcc caagtccacc aatgccagcc
     1201 aatctcgaat aaacgaaatt cataaacaat ggtgtaaacc ccaaaggcca agcttttcat
     1261 tacaaaatta atcagcatcg agccaatccc catagcaatg gaagcggctg catctttcga
     1321 caagtaattg cgatgctgtt gcttgtagcc cacataggct tcaattaaaa tcagcagaat
     1381 gaaaaacggg atggcatagg caacaggatc ttgctctgtt gggtaaagtt tgaaaatcga
     1441 caacaacata gtgtatgttt tattcattaa aggaacaaaa aaagggttga gaaatacaaa
     1501 tagatttgat actgattttc gtcacctttg cggctcttca acaagtggac ggaatgttgt
     1561 tgtgaaagta actataccct catcagaatt tgtgccctaa atgtggaa
//
LOCUS       DAOPUW010000516         1607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_86052_length_1607_cov_3.125000, whole genome shotgun sequence.
ACCESSION   DAOPUW010000516 DAOPUW010000000
VERSION     DAOPUW010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86052_length_1607_cov_3.125000"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PKJ43_08260"
     CDS             complement(<1..388)
                     /locus_tag="PKJ43_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72601.1"
                     /translation="MNNSPFKVLIISTLLITLLNACKKEDDNIDFRNEMRRFIIGLSH
                     YSKQQNPDFIVVPQNGQELITDTGEANGTIMSEYIEAIDATAREDMLFGYFDDDQPSP
                     SDFIEFMTSLCSLYTTKHKPALVIDYC"
     gene            complement(411..>1607)
                     /locus_tag="PKJ43_08265"
     CDS             complement(411..>1607)
                     /locus_tag="PKJ43_08265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ72602.1"
                     /translation="RKDDFGSLASLVHNSFEQQKKLTQEITDRQQAESQLRMLSKAID
                     QSPDSIIITNTKGEIEFCNTAVMKISGFEREEIIGSTPKIFSSGRKTREEYQELWHTI
                     TSGKIWEGEFLNKKKNGDLYWESTTISPVTNSQGKITHYLAIRKDITEQKMMTRELIK
                     AKERAEESDRLKSAFLANMSHEIRTPMNSIMGFASLLPDEDSKDIMAQYATIIYKNSE
                     QLVNIIDGIVLYSKLQTHQLSFNPTTFEVQRLLNELRNMFKTSDNYSSITLFFDTPTE
                     THDAITTDYEKLRQILYNIISNAFKYTHEGEIHVGYSYKENSTCEFYVSDTGIGIPKS
                     DITRIFERFYRGSNVNESTVRGTGIGLSIVKELVELMGGTIWAESHTEDFGHIRGSKF
                     SFKIPV"
BASE COUNT          396 a          299 c          345 g          567 t
ORIGIN      
        1 aacagtagtc gattaccaag gccggtttat gttttgttgt gtatagggaa caaagagagg
       61 tcataaactc aataaaatca gaaggtgatg gctggtcgtc gtcgaaatat ccgaacaaca
      121 tatcttcgcg ggcggtggca tcaattgctt caatatattc actcattatc gtgccgttgg
      181 cttcgcctgt gtcggtaatc aattcttgcc cgttttgtgg tacgacaatg aaatcggggt
      241 tttgttgttt cgagtaatga cttagtccga ttataaacct tcgcatttcg ttacggaagt
      301 caatattatc atcttccttt ttacatgcat ttaagagtgt aataagtaag gttgagatta
      361 tcaatacttt gaatggtgag ttattcatga ataacaagtt ggctcaacgg ctataccggt
      421 attttaaagc taaatttgct tccccttata tggccaaaat cttcggtatg actttcggcc
      481 cagatagttc cgcccataag ctcaacgagc tcttttacaa tacttaggcc aattccggtt
      541 cctctcactg tcgattcgtt gacattactt ccacgataga aacgctcgaa aatacgcgtg
      601 atatcagatt tgggaatgcc aatgcctgta tcgctcacat aaaattcgca tgtagagttt
      661 tctttatacg aatatccaac gtgaatttca ccttcgtggg tatatttgaa agcattggaa
      721 atgatattgt atagaatttg cctgagtttt tcgtaatcgg tagtaatagc atcatgcgtt
      781 tcagttggtg tgtcgaaaaa caacgtgatt gacgagtagt tgtctgaagt tttgaacatg
      841 ttcctaagtt catttagcag tcgttgcact tcgaatgttg tagggttgaa tgatagttgg
      901 tgtgtttgta actttgaata cagtacaatt ccatcgataa tatttaccag ttgctcagag
      961 tttttgtaga ttatagtggc atattgtgcc atgatgtctt tgctgtcttc atcgggcaaa
     1021 aggctcgcaa agcccatgat ggaattcatc ggggtgcgta tttcgtggct catattggcc
     1081 agaaaagcag atttcaaccg atcgctttct tctgcccgtt ctttggcttt tatcagttct
     1141 cgggtcatca tcttttgctc ggtaatgtct tttcgaatag ccaggtaatg agttattttt
     1201 ccttgggaat ttgttacagg cgaaatggtg gtcgattccc aatacagatc tccatttttc
     1261 tttttattta gaaactcgcc ttcccatatt tttccagaag taatggtatg ccaaagctct
     1321 tggtattctt ctcttgtttt acggcccgaa ctgaatattt taggagtgct gccgataatt
     1381 tcttctctct cgaatcccga aattttcatt actgctgtat tgcagaattc aatttcgcct
     1441 ttggtattgg taatgatgat tgagtcgggg ctttggtcta ttgctttaga aagcattctt
     1501 agttgacttt cagcttgttg cctgtcggtg atttcttgtg ttagtttctt ttgttgttcg
     1561 aaagagttgt ggacgagcga ggcaagcgat ccaaaatcgt cttttcg
//
LOCUS       DAOPUW010000517         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_86420_length_1603_cov_2.663437, whole genome shotgun sequence.
ACCESSION   DAOPUW010000517 DAOPUW010000000
VERSION     DAOPUW010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86420_length_1603_cov_2.663437"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="PKJ43_08270"
     CDS             <1..1209
                     /locus_tag="PKJ43_08270"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNZ72603.1"
                     /translation="EVTLTFANVSRLIGLDISVETIKNILLALEIEIVAETTESLTLK
                     VPPYRVDVQREADVIEEILRIYGYNNVAPGVKVNSTLQYSPSPDKEKIKNLVSDMLVS
                     AGFFELWSNSLTKASYYDSLQTFAPEATVKIYNPLSQDLNGMRQSLLFGGLEAIVRNT
                     NFQHPDLKLFEFGNTYFYKGGENLKSPADKYYEEMHLALFVTGQKQPENWVTRAEKSS
                     FFLLKSHVLNILKRLGVNVEKLKTEAISNDIIEDGIAYRRGDGKTFVEMGFVNSKLLR
                     ASGIDNEVYYADFNWDIVVQQVTNTKTAVSELPKFPSVRRDLALLVDENITFAQLKDV
                     AKRTEKLLLREVDIFDVYKGDKIPAGKKSYALSFVLRDDEKTMNEQTIERTMNRLIAM
                     FEKELGAKLR"
     gene            1285..>1603
                     /locus_tag="PKJ43_08275"
     CDS             1285..>1603
                     /locus_tag="PKJ43_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72604.1"
                     /translation="MMGLITPVYKIITPENSSSFENQWTSCLQQLINISARDALNPFR
                     VNVFVKSSGYEDYNAQMALVEAGMKREFGAQCPPFGVVMQEPDDPYLVTLEVGFVEKQ
                     VATI"
BASE COUNT          491 a          295 c          352 g          465 t
ORIGIN      
        1 gaagtaacac ttacatttgc taatgtttcc cgactaattg gtctcgatat ttcagttgaa
       61 acaataaaga atatcctttt agcccttgaa atcgaaatag ttgccgagac tacagaaagc
      121 ctcaccttaa aagtgcctcc atacagggtt gatgttcagc gagaagccga tgtaattgaa
      181 gaaattctaa gaatttatgg gtacaataat gttgctcccg gagtgaaggt taactcaacg
      241 ttgcaatatt cgccctcgcc cgataaagaa aaaattaaaa acctcgtttc ggatatgctg
      301 gtttcggcag gattcttcga actttggtca aattctctca caaaagcatc gtattacgat
      361 agcttgcaga ccttcgcccc tgaggcaact gtgaagatat acaatccgtt gagccaggat
      421 ttgaatggca tgcgtcagtc gttgctattt ggtggtctcg aggcaattgt tagaaatacc
      481 aatttccagc atcccgacct taagttattc gaatttggta atacctattt ttacaaaggt
      541 ggtgaaaacc ttaaatcgcc tgccgataag tattacgagg aaatgcacct tgccttgttt
      601 gttactgggc aaaaacaacc cgagaactgg gttacacgtg ccgaaaaatc ctcgttcttt
      661 ttacttaaga gccacgtgct gaatattctt aaaaggctgg gagtgaatgt cgaaaaactt
      721 aaaactgaag ctatctcaaa cgatataatc gaagacggga ttgcatatcg tcgtggcgat
      781 gggaaaacct ttgttgaaat gggcttcgtt aattcaaaac tgcttcgtgc ttccggtatc
      841 gacaatgaag tatattatgc cgattttaat tgggatattg ttgttcaaca ggtaactaac
      901 accaaaactg cagtttccga gcttcctaaa tttccttcgg tacgtcgcga cttggccttg
      961 cttgtcgatg agaatataac ctttgcacaa ttgaaggatg tggctaagcg gacagaaaaa
     1021 ttactcttgc gcgaagtcga tattttcgat gtttataaag gggataagat tccggctggt
     1081 aaaaaatcgt atgcattgag ttttgttctt cgcgacgatg aaaaaaccat gaacgaacaa
     1141 accatagagc gtactatgaa ccgccttatt gccatgtttg aaaaagaatt gggggcaaaa
     1201 ttgcgataac tttatttggg gcttgcttgt ttcttctgag gaaaaattaa aagaatttgt
     1261 caaaaaaatt ggagaacaat tataatgatg ggacttataa cgcctgttta taaaataatt
     1321 acaccagaaa atagtagtag tttcgagaac cagtggacaa gttgtttgca acaattgatc
     1381 aatatttctg ctcgggatgc attaaaccct tttcgggtaa atgtgtttgt aaaatcatca
     1441 ggctacgagg attataatgc ccagatggca cttgttgaag ctggcatgaa aagggaattt
     1501 ggggcacaat gtcctccatt tggggtagtg atgcaggaac ccgacgatcc gtatctggtt
     1561 actcttgaag tagggtttgt tgaaaaacaa gtggcgacaa tcg
//
LOCUS       DAOPUW010000518         1596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_87024_length_1596_cov_2.634653, whole genome shotgun sequence.
ACCESSION   DAOPUW010000518 DAOPUW010000000
VERSION     DAOPUW010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87024_length_1596_cov_2.634653"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..>1596
                     /locus_tag="PKJ43_08280"
     CDS             188..>1596
                     /locus_tag="PKJ43_08280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNZ72605.1"
                     /translation="MKLFPTSAISQIDKLTMQYEPITNIDLMERAATALFNRFVVSGV
                     FGKIIFFCGPGNNGGDGLALARLLCSLPDMFQPFVYLFDFGHSLSPSAKINLERLKAL
                     NKVQVETPQNIDELFVEKNAIIVDALFGAGLNRPLEGFATEVVKYMNDSGNQIIAIDI
                     PSGLLGEDNRNNVSNSIVKAHCTYTFQFPKISFLFPENSIYVGCWQLLDIGLHQLAIE
                     QTASNFHYIDCSDCSKFFKQREKFSHKGNYGHALLVAGAYGKMGAAVLSAKSCLRSGA
                     GLLTVHVPHKTYQVLQAAVPEAMCSIDQSDLMFTEVADLKPYAAIGVGPAIGLKVNAQ
                     RGLHNLISRVEVPLVLDADAINILGLNPDWLRFLPPNTILTPHPKEFERIAGSCDCAY
                     TRMEKALDMAKEFNIIVVLKGAHTIIALPSGNVWFNSTGNPGMATAGSGDVLTGVILG
                     LLAQGYTPSEAAILGVYLHG"
BASE COUNT          410 a          306 c          346 g          534 t
ORIGIN      
        1 gcaaactacg agttgttttc cctttttttt acataaaact cctgaattat tgttattttt
       61 agctttcatc tcaacctcgg caagattgta tagaaggttg tacatattgt tgtagttcaa
      121 ccaagttaat gctttgttga gtggtgaatt tgttgaaatg cttaacaatg ctaatatctt
      181 atttcatatg aagcttttcc caacatctgc aatttctcaa atcgataaac taaccatgca
      241 atatgaacca attaccaata tcgatttgat ggaacgtgct gctactgcac ttttcaatcg
      301 gtttgttgtt tcaggggtat tcgggaaaat tatttttttt tgtggcccgg gcaataatgg
      361 aggcgacggt ttggcccttg cccgtttact gtgtagcctc ccggatatgt tccagccgtt
      421 tgtttattta ttcgatttcg gacattctct ttccccttct gcgaaaatca accttgagcg
      481 cttaaaggca ctaaataaag ttcaggtaga aacccctcaa aatatcgatg aattgtttgt
      541 tgaaaaaaat gcaattattg ttgatgcact ttttggagcc ggacttaatc gtcccctgga
      601 gggttttgcc acagaggttg taaagtatat gaatgattca ggtaatcaaa ttattgcaat
      661 tgatattcct tcagggcttt tgggcgaaga caaccggaat aatgtttcca attctatcgt
      721 gaaagctcac tgtacctata catttcagtt ccccaaaatc agttttcttt ttcctgagaa
      781 tagcatttat gtcggatgtt ggcaattgtt agatatcggt ctgcatcagt tagccattga
      841 gcaaacagct tctaattttc attatatcga ttgttctgat tgttctaaat tctttaaaca
      901 gcgcgaaaaa ttctcgcata aaggaaatta cgggcatgcc ttgttggttg ccggagctta
      961 tggtaaaatg ggagctgcgg ttttgtctgc caaatcgtgt ttgcggtcag gggcaggctt
     1021 attaaccgtt catgttccgc acaaaacata tcaggttttg caggctgctg ttcccgaagc
     1081 aatgtgtagc atcgatcaat cggacctgat gtttactgaa gttgcagatt tgaagccata
     1141 cgcagcaata ggggttggtc cggctattgg gttaaaagta aatgctcagc gtggtcttca
     1201 caatcttatt tcaagggttg aagtgcctct ggttctcgat gccgatgcca taaatatttt
     1261 agggttgaat ccggattggc ttcgtttttt acctcccaat actattctta ctcctcatcc
     1321 caaggagttt gaacgcattg ccgggagttg cgactgtgca tacactcgca tggaaaaagc
     1381 tcttgatatg gctaaagaat tcaatataat tgttgtgtta aaaggagcgc ataccataat
     1441 agcattgcct agcggtaatg tttggttcaa ctcgacagga aatccgggga tggctactgc
     1501 tgggagtgga gatgttctta ccggtgttat tcttggcctt cttgctcagg gatatactcc
     1561 gtctgaagca gcaatacttg gtgtttactt gcatgg
//
LOCUS       DAOPUW010000519         1594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_87206_length_1594_cov_2.692658, whole genome shotgun sequence.
ACCESSION   DAOPUW010000519 DAOPUW010000000
VERSION     DAOPUW010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87206_length_1594_cov_2.692658"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="PKJ43_08285"
     CDS             complement(<1..1027)
                     /locus_tag="PKJ43_08285"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNZ72606.1"
                     /translation="MEKRPKRIGILTAGGDCPGLNAAIRGVGKTAIVEHGMEVIGFVS
                     GYTGLIHKEYTVLEESKLSGILTLGGTILGTSREKPFKNDLNNDDINKPELIVNNYRE
                     LGLDCLVCIGGNGTMKTAARLTNMGLNVVGIPKTIDNDVWGTDITFGFDSAIAIATEA
                     IDRLHTTANSHQRAMVIEVMGHSAGWIALYAGMAGGGDVILIPEIEYNIRTVCKKVEK
                     RFAEKKPYSIVVVAEGIKVPSSKTAGSYICECISEYTGVEARETRLGYIQRGGSPTTM
                     DRILATRYGSYAVEIIAEGKYGKMVAMKDGHLTTIPLNEVENKLNLVSTDHPLVKMCR
                     NMGVSFGD"
     gene            complement(1084..>1594)
                     /locus_tag="PKJ43_08290"
     CDS             complement(1084..>1594)
                     /locus_tag="PKJ43_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility protein GldB"
                     /protein_id="HNZ72607.1"
                     /translation="CIFYSYLGVPVYKRYNMNPGKIVPDVFYSMYLSDFTFSDSINNL
                     LANMVYQGKGLYFTKAMCPEIPDTLIMGYSSKQLKWCHENEGKMWTYLVERKLLYDNE
                     RLTLQKYIGDSPFTTTFSSESPGRTGNWLGYRIVESFMKNNPDITLNQLLAMNNSQQI
                     LAMSKYFPD"
BASE COUNT          417 a          395 c          306 g          476 t
ORIGIN      
        1 catcgccaaa agatacgccc atgtttcggc acattttcac caaaggatgg tcagtagaga
       61 ccaaattgag tttgttttcc acctcgttga gcgggatggt tgtcaaatgc ccgtctttca
      121 tggcgaccat ctttccatac tttccttcgg caattatctc aacagcatac gagccatagc
      181 gggtagccaa aattctgtcc atagttgttg gactgccgcc gcgctgaata tatcccagcc
      241 tggtttcacg ggcctcaacg ccggtatatt cactaataca ttcacaaatg tagcttccgg
      301 ccgttttcga cgaaggaacc tttattcctt cagcaaccac aacaatagag tagggtttct
      361 tttcggcaaa gcgcttttct acttttttac ataccgtacg gatgttgtac tctatctcgg
      421 ggataaggat tacatctccc cctccggcca ttccggcata aagggcaatc catccggcac
      481 tgtgccccat aacttcaatt accatagcgc gctggtgcga gttggcggta gtatgcaagc
      541 ggtcaatcgc ttcagtagca atggctatag ccgagtcgaa accaaaagtg atatcggttc
      601 cccacacatc gttgtcaatg gttttgggaa ttcctaccac attcaacccc atattggtaa
      661 ggcgtgcagc ggttttcatg gttccgtttc ctccgatgca caccaaacag tcgagcccta
      721 attcacgata gttgttcaca atcaactcgg gcttgtttat atcatcattg tttaaatcgt
      781 ttttaaacgg tttttcgcgc gaggtaccta aaatggtccc tcccaacgtg agtatgcccg
      841 aaagtttcga ctcctcgagc actgtatatt ctttatgtat cagccctgtg taccccgaaa
      901 caaaaccaat tacttccatc ccgtgctcta caatggctgt tttccctacg ccccgtatgg
      961 ctgcattcag ccccgggcaa tcgcccccgg cggtcaatat cccaattcgt tttggccttt
     1021 tttccatctt ctctaaattt tacccaaagt tacctaaacc atattgtttt tcgatacatt
     1081 tttttaatca gggaaatatt tcgacatggc aagaatctgc tgcgaattat tcattgcaag
     1141 caactgatta agtgttatat ccggattatt tttcataaac gactctacaa ttctatatcc
     1201 cagccaattg ccagtccggc ccggtgattc gctcgaaaat gtagtggtaa atggtgaatc
     1261 gccaatgtat ttctgaaggg tcaaacgctc gttgtcgtat aataatttcc gctcgacgag
     1321 ataggtccac atttttcctt cattttcgtg gcaccatttt aattgcttcg aactgtagcc
     1381 cataattaac gtgtcgggta tttcagggca cattgctttt gtaaaatata aacctttccc
     1441 ctgatacacc atattggcca acaggttatt tattgaatcg ctgaaagtaa aatcggataa
     1501 gtacatagaa taaaaaacat caggaactat ttttccagga ttcatattgt agcgcttata
     1561 aaccggtacg ccaagataag aatagaaaat acaa
//
LOCUS       DAOPUW010000520         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_87354_length_1592_cov_3.364997, whole genome shotgun sequence.
ACCESSION   DAOPUW010000520 DAOPUW010000000
VERSION     DAOPUW010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87354_length_1592_cov_3.364997"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PKJ43_08295"
     CDS             <1..494
                     /locus_tag="PKJ43_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ72608.1"
                     /translation="ITIAGGGNPVVVQSGATVRFIAGQSIQLLPGFHAQESSSVHAFI
                     TTDGSFCDVSPTPSIVQLPPVAEKSASQNSTIEEANDQPFQPHLKAYPNPSNGEFTLE
                     INNFDEQTEIVVFNMLGMVVSKIKNNNSSQVDINLPYWAKGIYNIRVTDGLREKSVKM
                     IIQ"
     gene            535..>1592
                     /locus_tag="PKJ43_08300"
     CDS             535..>1592
                     /locus_tag="PKJ43_08300"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNZ72609.1"
                     /translation="MKAIKSTVILALIFCFTLPVMAQLGNIKSIGKKVVNSTEKAVTS
                     QSGQNKSDTPKVDQSEAKASSPTANSQVLFVSKTKGNNKNDGSQVAPLKEIDKAIELS
                     APGGEIRVAGGRYSGTLNVGFFVSDKPVRIYGSYDEDFAVQDITAHPTLLQPDNESAR
                     STRKCVMQFTKDVAGTELDHLVFDGGERNAYHATDGVVEGIEGGRLLPPTEKPPVANP
                     TVGEPLLQFVSATTGGDVTIQNCVFVNGASFALQAGHRSGKFTVKNNVFVANKMAAIE
                     IFGTCASTGGPNTLALCGEVEVAYNTIIFTWSRLKDFLDMGYGVRIMTKCDYNIHHNI
                     IGASILAGVDHTRFNKDE"
BASE COUNT          490 a          334 c          356 g          412 t
ORIGIN      
        1 caattaccat agcaggcgga ggtaacccgg tggtagttca aagtggggca actgtacgtt
       61 tcattgcggg acaatcaatt cagcttttgc caggatttca tgcacaggag tcgagttctg
      121 ttcatgcgtt cattacaacc gatggctctt tttgcgatgt gtctccaact ccaagtattg
      181 tacagctacc tcctgtggct gaaaaaagcg cttctcaaaa ttcaacaatt gaagaggcaa
      241 acgatcagcc ttttcagcct cacttgaaag cgtaccccaa tcctagcaat ggagagttca
      301 ccctcgaaat caacaatttt gacgaacaaa ctgaaatcgt tgtttttaac atgctgggca
      361 tggttgttag caagataaaa aacaataatt cttcacaggt tgatataaac ctgccttatt
      421 gggctaaggg aatttacaac atacgtgtaa ccgacgggct tagagagaaa tcggtaaaaa
      481 tgattataca gtaagtcttc ttaaaaaaaa tcgatatcca aaaaatcaac aaaaatgaaa
      541 gcaattaaat ccacagtaat tcttgcccta atcttttgct ttacgctacc agtaatggca
      601 cagttgggaa acataaaaag cattggcaag aaggtagtaa acagcaccga aaaagctgtt
      661 acatcacaat caggtcaaaa caaatcggat accccaaagg tagatcaatc ggaagcaaag
      721 gcttcatcgc caactgcaaa ttcgcaagta ttgtttgttt cgaaaacaaa ggggaacaac
      781 aaaaacgatg gttctcaggt agctccgtta aaagagatcg acaaagccat tgagctttca
      841 gcgcccggtg gcgaaatacg tgttgccgga ggaaggtatt cggggacatt gaacgtggga
      901 ttttttgtta gcgacaagcc agtcagaatc tacggtagct acgatgaaga ttttgccgtt
      961 caggatatta cagcccatcc aacacttttg caacccgaca acgaaagcgc ccgttcaaca
     1021 cgtaaatgcg tgatgcaatt caccaaagat gttgccggta cagaacttga tcatctggta
     1081 ttcgatggag gcgaacggaa tgcttaccat gcaaccgatg gggttgttga aggtattgag
     1141 ggtggtaggc tattgccccc tactgaaaaa ccacctgtag caaacccaac tgttggtgaa
     1201 ccgctccttc agtttgtcag tgctaccaca ggcggcgatg tgactattca aaattgtgta
     1261 tttgttaatg gtgccagttt tgccttgcag gcaggtcatc ggagtggaaa atttaccgtg
     1321 aagaacaatg tttttgtagc caataaaatg gctgcaatcg aaatttttgg aacctgtgcc
     1381 agcactggcg gccccaatac cctggctctt tgtggtgaag ttgaagtggc ttataacacc
     1441 atcattttca catggagccg tttgaaagat tttctcgata tggggtatgg agttaggata
     1501 atgaccaaat gcgattacaa tatccaccac aatattattg gcgcttccat tttggcaggt
     1561 gttgaccata cccgtttcaa caaagacgaa tg
//
LOCUS       DAOPUW010000521         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_87560_length_1590_cov_2.518567, whole genome shotgun sequence.
ACCESSION   DAOPUW010000521 DAOPUW010000000
VERSION     DAOPUW010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87560_length_1590_cov_2.518567"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PKJ43_08305"
     CDS             <1..699
                     /locus_tag="PKJ43_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNZ72610.1"
                     /translation="KLVRRIARKIAFEEPVDLDVKTEHIKEYLGIPRFNAEKYQGNEF
                     AGVVTGLAWTAVGGEILFVESSLSKGKGALTLTGNLGEVMKESAMLALEYLKAHADVL
                     SIDPEVFSNWNVHIHVPEGAIPKDGPSAGVTMVTSLASSFTQRKVKRNLAMTGEITLR
                     GKVLPVGGIKEKILAAKRAGITDIILSEENRKDIEEIKEIYINGLNFHYVKTIGEVLH
                     LALMDEKVEKPLSI"
     gene            complement(774..1301)
                     /locus_tag="PKJ43_08310"
     CDS             complement(774..1301)
                     /locus_tag="PKJ43_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ72611.1"
                     /translation="MNTLSNGFVKLRALEPDDLELLYAWENDPEVWRVSNTHAPISKY
                     MLANYIKSSDQDIWESRQLRLVIELLDNQQVGSIEIFDFDPFHLRAGVGVIIFADEER
                     RKGVASQALRLLMNYAHNELGIYQLYANVAETNEASIALFRKLGFLQTGCKTDWLRIP
                     GKGWECELIFQCQFS"
     gene            complement(1298..>1590)
                     /locus_tag="PKJ43_08315"
     CDS             complement(1298..>1590)
                     /locus_tag="PKJ43_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72612.1"
                     /translation="FPLTVWLIEKKRGLFFNIFNVLGHRFSWVGYHAVNTEMFLGLPV
                     IKPGVIKTTDLMEVGKRNFDAIDRANMLYARDYSVAKDFEFVIKAFKKLGQS"
BASE COUNT          494 a          356 c          319 g          421 t
ORIGIN      
        1 aaacttgtca ggcgcattgc ccggaaaatt gcctttgaag aacccgttga tctcgatgta
       61 aaaacagaac acataaaaga atacctcggc attccacgct tcaacgcaga aaaataccaa
      121 gggaacgaat ttgccggtgt agtaacggga ctagcctgga ctgcagttgg gggcgaaata
      181 ctctttgttg aatcgagttt gagcaaaggg aagggagcac taacgcttac cggaaattta
      241 ggtgaagtta tgaaagaatc ggctatgctg gcattagagt acctaaaagc ccatgccgat
      301 gtgttgagca ttgatccaga agttttttcc aactggaacg tacatattca cgtacccgaa
      361 ggtgctattc ctaaagatgg tccatcagcc ggggttacca tggtaacgtc gctggcttct
      421 tcattcacac agcgaaaggt gaagcggaac cttgccatga ccggtgaaat taccctcagg
      481 ggaaaagttc tcccggtggg tggaatcaaa gaaaaaatac ttgccgctaa acgtgctgga
      541 attactgata ttattctttc tgaagaaaac agaaaagata ttgaagaaat aaaagaaatc
      601 tacattaatg gactgaactt tcactatgtg aaaacaatag gcgaagtact ccaccttgca
      661 ttaatggacg agaaagttga aaaaccactt tcaatctgat taattttgga gaaacacgac
      721 cgttgaggtg attacttttg catgaatgcc ctgagcttgt aattagctga ttgttaagaa
      781 aactggcatt gaaaaataag ttcgcactcc caccctttac caggaatgcg gagccaatcg
      841 gtcttacaac ccgtctgcaa aaagcccaac ttccggaaca gtgcgatgct cgcttcgttt
      901 gtttctgcaa cgtttgcata taactgatag ataccaagtt cgttgtgtgc ataattcatt
      961 aaaagacgaa gagcctgact agcaacaccc tttcttcgct cctcgtcggc aaaaataatt
     1021 acacctaccc cggcacgaag atgaaacgga tcgaaatcaa aaatctcaat agagccaacc
     1081 tgctgattat ctaacaattc aataacaagc cgtaattgtc ggctttccca aatatcttga
     1141 tcagatgatt ttatataatt ggccagcatg tatttcgata ttggcgcatg agtgttgctt
     1201 acacgccaaa cctccgggtc gttttcccaa gcatagagga gctccagatc atcgggctcc
     1261 aacgccctaa gttttacaaa accatttgac aaggtattca tgattgtcca agttttttga
     1321 atgctttaat gacaaactca aaatcttttg ctacactgta atccctggca taaagcatgt
     1381 ttgccctatc gattgcatca aagtttcttt tccccacctc cattaaatca gtagttttaa
     1441 tcactccggg ttttataact ggcaacccaa gaaacatttc ggtattcacc gcgtgatacc
     1501 ccacccaact gaatctgtgc cccaacacgt taaaaatatt gaaaaacaga cctctttttt
     1561 tctcgattag ccataccgtc aacgggaaag
//
LOCUS       DAOPUW010000522         1588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_87719_length_1588_cov_2.720157, whole genome shotgun sequence.
ACCESSION   DAOPUW010000522 DAOPUW010000000
VERSION     DAOPUW010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87719_length_1588_cov_2.720157"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PKJ43_08320"
     CDS             <1..517
                     /locus_tag="PKJ43_08320"
                     /inference="COORDINATES: protein motif:HMM:NF040309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6051 family protein"
                     /protein_id="HNZ72613.1"
                     /translation="PSNTFDKSKLFIFCGGPTFDKMNGDSKYIMDSLAFKSLLELKHR
                     QKLMQIKKQVFATKDKTLIDTWPGLELMMYEKRDRRKREALFDAMKDRLYVVALEKDT
                     VMPVDGIVKTVRGKKGNIEVPVEVIDFPYTYSHEQPFPVGDDKIQNLVDRSFTVVFDK
                     AIDFFQEPASL"
     gene            complement(496..867)
                     /locus_tag="PKJ43_08325"
     CDS             complement(496..867)
                     /locus_tag="PKJ43_08325"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HNZ72614.1"
                     /translation="MEKYEKYSQYYNENSFWVKIKKLAGKASSSLLYYALILYYCLIS
                     SEVSLKNKSLIIAALGYLIFPIDALPDFIPALGLTDDLTAIMLVYNKIKGSLTPDIER
                     KAKSKVNDLTASLSGHKEAGS"
     gene            1050..1457
                     /gene="ssrA"
                     /locus_tag="PKJ43_08330"
     tmRNA           1050..1457
                     /gene="ssrA"
                     /locus_tag="PKJ43_08330"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          466 a          301 c          364 g          457 t
ORIGIN      
        1 cccttcgaat actttcgata aatcaaagct ctttattttt tgcggaggcc cgacttttga
       61 taaaatgaac ggcgattcga aatatattat ggattcccta gcgtttaaaa gcctgctcga
      121 attgaagcat cgccaaaagt tgatgcaaat caaaaaacaa gtgtttgcca caaaagataa
      181 aacgttgatt gatacatggc ctggacttga attaatgatg tatgaaaaac gcgatcgcag
      241 aaaacgcgaa gccttattcg atgcaatgaa agaccggctt tatgtagtgg ccctcgaaaa
      301 ggatacggta atgcctgtag acgggattgt taaaaccgtg cgagggaaaa aggggaacat
      361 tgaagtgcct gtcgaggtaa ttgattttcc gtatacctac tctcatgagc aaccttttcc
      421 tgtgggcgac gataagatac agaaccttgt agaccgcagt tttacggtag tttttgacaa
      481 ggcaatcgat ttttttcaag aaccagcttc tttgtgaccc gataagctgg cagttaaatc
      541 gttgactttc gatttggctt tacgctcgat gtcgggggtt aacgaccctt taatcttatt
      601 atacacaagc atgattgctg ttaagtcgtc ggtaaggccg agggcaggaa tgaagtcggg
      661 tagggcatca attgggaaaa tcagataacc aagagctgca ataatcaacg atttgttttt
      721 taaacttact tcgctactga tgaggcaata atataagatt aacgcatagt agagcaacga
      781 cgaactggcc tttcctgcaa gttttttaat cttgacccaa aaactgttct cattgtagta
      841 ttgcgaatat ttctcgtatt tttccatgcc ttacacgaag ttttacgggg taaaaatacg
      901 aaagaatagg gtgctgtcgc aaaaggaata ctattttgag aaagattgtt cctatttgtc
      961 gtcaggaagt tatttgtaaa gtggttttta aaacgttgat ggtatattgt aattcagttg
     1021 caaatgtgta attttgcagc accacaattg gggttgactg gttttgacag caggccggtg
     1081 tggtatgtaa gcatgcaggg atttgactgt ttgcccttga ttaaatggtc acaacaataa
     1141 ttggcgaaga aaactttgct cttgctgctt aatcgaatta cagtagattc aagctcctct
     1201 tcggaggatt tccggcatta gatgctggaa caagacgttt cccgggcggt caatacctct
     1261 caccaacctg aatcggaagc gctcgtaatc agaggttagt gtcgcgcagg cttcggtcgc
     1321 gatgcgaaat taagaagcta aggtaaacgt cggtggctct gatccagcgt ttacacgaaa
     1381 actaagtcag gctaagcatg tagaaagcgt atggctcccc tgtttggacc cgggttcgaa
     1441 tcccggcagc tccacaaaag actctggctc aataagctag agtctttttt ctttcttgat
     1501 aaattatacc agatgcaatt ctaaaacagt ccaaacctat tcatgtataa tgccgatgaa
     1561 tagaaatgtt cagtacaatc gagtgggc
//
LOCUS       DAOPUW010000523         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_88669_length_1577_cov_2.787779, whole genome shotgun sequence.
ACCESSION   DAOPUW010000523 DAOPUW010000000
VERSION     DAOPUW010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88669_length_1577_cov_2.787779"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..>1577)
                     /locus_tag="PKJ43_08335"
     CDS             complement(178..>1577)
                     /locus_tag="PKJ43_08335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNZ72615.1"
                     /translation="YRDNQPLMGKSADSDYPLYNIGEELTGTYHCVITNTTIPDLQLR
                     SAPIIIQFKPLGQYPPSDILLSNNSVEENQSIGTIVGSFNAVDNDLGDLHFFSLVNGE
                     GSEDNNCFFLAANQLHTSLAFDYEEKNMYHIRIKCIDRAGLIFEKPFLIEIRDVPELL
                     PNNIELSHNTVDEFLPSGILIGTFSAFDDEVEITEPMEYQLNENPDTDFASFYIVNNE
                     LHSLQSFNRLEKSSYTISVTCVNQNDYSYEESFTIDVNKSGETLPADILLSSNTINEN
                     FPVGTFIGSLTTTGNPQSTAYDYTYTFVTGEGDSDNDRFFLAANQLHAAQEFNFETKS
                     SYSIRILSTDKWNNTMEKSFTIMVVDLDDTHSVLIPDAFSPNNDGKSDFFEIPGIENY
                     NNAVIDIYNRTGKLVYHSDYYGLTTEGETKNLWDGRMSAHGQTSSNLLPEGYYFVVLK
                     TTANTVFKKTVFLKH"
BASE COUNT          403 a          288 c          378 g          508 t
ORIGIN      
        1 catatactga ctgtacaagg gatcttgctg ggcaaacaac ctgcagggca gtacccatag
       61 caaagccagt aatacaatag ccgcaattgg gttgtgccgt ttcatgatgg ttgattattt
      121 tgagttttgt tattatcaag agaaatcata agcgatgtgt tttatttgtg gcgactttta
      181 atgcttcaag aaaactgttt ttttgaatac cgtgttggct gttgttttca gcacaacaaa
      241 ataatatccc tcgggcaaaa ggttgctgct tgtttgtccg tgggccgaca ttcgtccgtc
      301 ccaaaggttt ttggtctcgc cttcggtggt tagtccgtag tagtcgctgt ggtaaactaa
      361 ctttcctgtg cggttgtata tatcgatcac ggcattgtta tagttttcaa ttccagggat
      421 ttcgaaaaaa tcgcttttcc cgtcgttatt gggtgaaaag gcatcaggga taagcactga
      481 gtgggtatca tcgaggtcga caaccataat cgtgaagctt ttttccatcg tattgttcca
      541 tttatcggtc gataaaattc ggatggaata tgatgacttg gtttcgaaat tgaactcttg
      601 ggcagcgtgt aattggttgg cggctagaaa aaaacgatcg ttgtcgctgt ctccctcgcc
      661 tgttacaaac gtataggtat agtcgtaagc ggttgattgt gggttgcctg ttgtggtaag
      721 gctcccaatg aacgtaccta ctggaaaatt ttcgttgatg gtgttcgacg agaggagaat
      781 gtctgctgga agggtttcac cgcttttgtt tacatcgata gtgaaacttt cctcataact
      841 gtagtcgttt tggttgacac aggttacaga aatagtatag ctactttttt ctaatcgatt
      901 gaacgattgt aaactgtgta gctcgttgtt aacaatgtaa aacgacgcaa aatcggtatc
      961 agggttttca ttcaactgat attccattgg ctcggtaatt tcaacttcat catcgaaagc
     1021 agaaaacgta ccgataagaa ttccggaagg aagaaactca tccaccgtgt tgtgtgatag
     1081 ctcaatattg tttggaagga gttccggcac atcgcgaatc tcgatcagga atggtttctc
     1141 gaagattaaa ccagccctgt cgatgcactt aatgcggatg tggtacatat ttttttcttc
     1201 gtaatcgaag gctaatgatg tatgtagctg gttggctgcc agaaaaaagc agttgttgtc
     1261 ttcgcttcct tcaccattaa ctaatgaaaa aaagtgaaga tcgccaaggt cgttatctac
     1321 tgcattgaat gagcctacaa tggttcctat cgattggttt tcttctacac tgttgtttga
     1381 taacaggatg tctgacggcg ggtattggcc taatggtttg aattgaatga ttataggtgc
     1441 cgatcgtagt tgtaaatcgg gtattgttgt gttagtaatc acacaatggt atgtgccggt
     1501 taattcttcg ccgatgttgt acagcgggta atcagagtct gctgatttgc ccatcaaagg
     1561 ctggttatcg cgatacc
//
LOCUS       DAOPUW010000524         1572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_89080_length_1572_cov_3.604483, whole genome shotgun sequence.
ACCESSION   DAOPUW010000524 DAOPUW010000000
VERSION     DAOPUW010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89080_length_1572_cov_3.604483"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1572)
                     /locus_tag="PKJ43_08340"
     CDS             complement(<1..>1572)
                     /locus_tag="PKJ43_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016779098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNZ72616.1"
                     /translation="AVNDEYHYIILSDRGVDENHVPIPSLLAVSAVHHYLIEKRKRMQ
                     IAVVVETAEPREVMHFALLFGYGATAINPYLSFAVLDDLVKKNDLQLDFHTAEKNYVK
                     SINKGLLKVLSKMGISTLRSYRGAQIFEAVGIASDVLNKYFRGTTSRIEGIGIEDIAK
                     ESIASHKKAFLANDDKDLENKGLYAYRKDGEYHAWNPETISTLQLATRLGSYKKFKEY
                     TNIVDNKPNPVFLRDFMQFKKGKSIPIDKVEPASEITKRFVTGAMSYGSISREAHEAM
                     AIAMNILGGKSNTGEGGEDPLRYKIGEDGLNRRSAIKQVASGRFGVTTEYLVNADELQ
                     IKIAQGAKPGEGGQLPGYKVDQIIAKTRHSIPGISLISPPPHHDIYSIEDLAQLIFDL
                     KNVNPQARVSVKLVSESGVGTIAAGVAKAKADMILISGCEGGTGASPSSSIKYAGLPM
                     ELGLAETQQTLVMNNLRGQVRLQADGQLKTGKDIIHAALMGAEEYGFATSALIVLGCV
                     MMRKCHQNTCPVGVAT"
BASE COUNT          390 a          343 c          334 g          505 t
ORIGIN      
        1 tgttgccaca cctacaggac aagtattctg atggcatttg cgcatcataa cacatcccag
       61 aacgattaat gcgctagtgg caaaaccata ctcttcggca cccattaatg cggcatggat
      121 gatatctttt cctgttttaa gctgtccatc ggcctgaagt cgaacctgac ctctcaggtt
      181 gttcataacc agtgtttgct gagtttcggc taaacctaat tccattggga gcccggcata
      241 ttttattgaa cttgatgggc ttgcgcctgt tccgccttcg cacccgctga tgaggatcat
      301 gtcggctttt gcttttgcaa cacctgcggc aatggtgcca actccgcttt ctgaaaccag
      361 ttttacgctg acacgagcct gtggattaac atttttcagg tcgaaaatca attgagccaa
      421 gtcttcaatc gagtagatgt cgtggtgtgg tggtggcgaa atgagtgaaa ttcccggaat
      481 tgagtgacgg gtttttgcaa taatctgatc tactttgtag ccaggtaact gtccgccttc
      541 gcccggtttt gcgccttgtg ctattttaat ttgcaactcg tcggcgttca ctaaatattc
      601 agttgtaaca ccaaaacggc cagatgcaac ttgtttaatg gcactgcggc ggtttaatcc
      661 gtcttcgcca attttatatc gcaaaggatc ttcacctcct tctccggtat tactttttcc
      721 acctagaatg ttcattgcaa tggccatcgc ttcgtgagct tcgcggctta ttgatccgta
      781 tgacattgct ccggttacga aacgttttgt aatttcagat gccggttcaa ctttgtcgat
      841 aggaatagat tttccttttt tgaattgcat gaaatcgcgg aggaaaaccg gatttggttt
      901 attatcaaca atattggtgt actctttaaa ctttttgtag ctgcccaagc gggtagccaa
      961 ctgaagtgta gatattgttt caggattcca tgcgtggtat tcgccatctt tccgataagc
     1021 gtacagacct ttgttttcaa gatctttatc gtcgttggcc aagaaggctt ttttgtgcga
     1081 ggcaatcgat tcttttgcaa tatcttcaat cccgattcct tcgattcgtg atgttgttcc
     1141 cctgaaatat ttattcaaaa catcggaagc aatcccaaca gcctcaaaaa tttgggcacc
     1201 acggtagctt cgaagtgttg aaatacccat tttcgaaagg acttttaata atcctttatt
     1261 gattgatttg acgtagtttt tttcggcagt atgaaaatca agttgaagat cattcttttt
     1321 aactaaatcg tcaagaacag cgaacgatag gtatgggttg attgctgttg caccatatcc
     1381 aaagagcaaa gcaaaatgca ttacttcgcg tggttcggca gtttcaacaa caacggcaat
     1441 ttgcatcctc ttcctttttt caatcaggta gtggtgaact gcagaaacag ctaataatga
     1501 tggtattgga acatggtttt cgtcaacacc cctgtcgctt aaaatgatat agtgatattc
     1561 gtcgttaacg gc
//
LOCUS       DAOPUW010000525         1572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_89104_length_1572_cov_2.524720, whole genome shotgun sequence.
ACCESSION   DAOPUW010000525 DAOPUW010000000
VERSION     DAOPUW010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89104_length_1572_cov_2.524720"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1572
                     /locus_tag="PKJ43_08345"
     CDS             <1..>1572
                     /locus_tag="PKJ43_08345"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNZ72617.1"
                     /translation="VGFEPFEVSFNLVSDTVINIELELNAEIQEITVYGEAARKVHNT
                     EMSMVELPVIQVQKIPLFMGEPDLLKVLQLLPGVKSGTEGTSGIYVRGGGPDQNLFLL
                     DGVPVYNVNHLLGFFSVFNPSAIKTVKLYKGGFPARFGGRLSSVIDINMKEGNMKEFE
                     GEASLGIISSRLSLEGPIVKNKTSFIVSGRRTYGDLLLQPFLKLSAGDNLRAGYYFYD
                     LNAKVNHIFSERSRLYLSGYFGQDKLYVKDKSEYTYESVTYKSRNEVNIGWQNATGAL
                     RWNYLFSNKLFGNFIATYSKYNFFISYDYRNSSSDSPESRDFFKYLSGIDDWSAKAEF
                     DYFPVPQHAVKFGSGYIYHTFEPGVTRIQSAANNEPDGDFNLELGNDPIYAHEAYLFA
                     EDNFDITSRLKANIGLHLLGFSVQDVFYPSLEPRASLRFLVNEQFSLKASYSKMSQNI
                     HLLSSSTISLPTDLWLPTTKRIKPQKSHQYAVGAFFKLPHDLDFSVEGYYKTMTNLIE
                     YKDGASYILSGKGWED"
BASE COUNT          441 a          311 c          345 g          475 t
ORIGIN      
        1 gttggttttg agccgtttga agtctcattt aacttggtaa gcgatacagt aatcaatatc
       61 gaattagaat taaacgccga aattcaggag ataaccgtat atggcgaagc agcccgcaaa
      121 gttcacaata ccgaaatgag catggttgag cttcctgtaa ttcaggtgca aaaaattcct
      181 ctttttatgg gagaacctga tttacttaaa gtgttgcagc tgcttcccgg ggtaaaatca
      241 ggaacagaag gcacaagtgg tatttacgtt cgaggtggcg gccccgacca aaaccttttt
      301 ttgctcgacg gggtacctgt gtataacgtc aatcatttat tgggcttttt ttcggtgttt
      361 aacccttcag caataaaaac tgttaagcta tacaaaggag gcttccctgc ccgttttgga
      421 ggccggcttt catcggtcat cgatatcaat atgaaggaag gcaacatgaa ggaattcgaa
      481 ggtgaagcgt ctcttgggat catctcatcg cggctttcac ttgaagggcc aattgtgaaa
      541 aacaagactt cgttcatcgt ttcgggaagg cgaacgtatg gcgatctttt gttgcagcca
      601 ttcctgaaac ttagtgccgg cgataactta agggctggct attattttta cgacctgaac
      661 gctaaagtaa atcacatttt ttcagaacgg agccggcttt atttaagcgg atattttgga
      721 caagataagc tttatgtaaa agacaaatcg gaatacactt acgaatcggt tacctataaa
      781 agtcgtaacg aagttaacat aggctggcaa aatgccacgg gtgctttgcg ttggaactat
      841 ctgttttcaa ataaattatt tgggaatttt atcgctactt acagcaagta taattttttt
      901 attagttacg attaccgcaa tagcagttcc gattcgcctg aatcgaggga ttttttcaag
      961 tacctttccg ggatcgacga ctggtcggca aaggctgagt ttgattactt tccggtgccc
     1021 caacatgctg tgaaatttgg ttctggatac atttatcaca cttttgaacc tggggtaacc
     1081 cgaattcaat cggcagccaa taatgaaccc gatggtgact ttaaccttga gttggggaac
     1141 gatccgattt atgcccacga agcctatttg tttgccgaag ataatttcga tatcacttct
     1201 cgtttgaaag caaatatcgg cttacacttg cttggtttca gcgttcagga tgtattttat
     1261 ccttcacttg aaccgcgggc tagtttgcgt ttccttgtaa atgaacaatt ctcgctgaaa
     1321 gcttcgtaca gcaaaatgtc acagaatatc catctgctta gcagttctac aataagtttg
     1381 cccaccgatt tgtggttgcc taccactaaa cgaattaagc ctcaaaaatc gcatcaatat
     1441 gcggtaggtg cattttttaa attaccacat gaccttgatt tttcagtaga agggtattac
     1501 aaaaccatga ctaatctgat tgagtacaaa gatggtgctt cgtatatttt gtctggcaaa
     1561 ggatgggaag ac
//
LOCUS       DAOPUW010000526         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_89177_length_1571_cov_3.294855, whole genome shotgun sequence.
ACCESSION   DAOPUW010000526 DAOPUW010000000
VERSION     DAOPUW010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89177_length_1571_cov_3.294855"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..>1571
                     /locus_tag="PKJ43_08350"
     CDS             186..>1571
                     /locus_tag="PKJ43_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNZ72618.1"
                     /translation="MKQIKSLTAFFLVVISIFLSIGCEKTEKWKNPNLPVEKRVNDLV
                     SRMTLEEKISQMMDVAAPIERLGIPAYNWWNEGLHGVARAGIATVFPQAIGVAATFND
                     SLLLVEAQVISDEFRARYNNYIKKNERDRYKGLTIWSPNINIFRDPRWGRGQETYGED
                     PYLTSRLGVAFVKGLQGNNPNYLKTIATPKHYVVHSGPEKDRHVFDADVTERDFMDTY
                     LPAFEATIKEGKAYSIMGAYNRFRGESCSGSDTLLNQILRKQWGFKGYVVSDCGAIDD
                     IYLNHKIVPTGAEAAAIGVLGGCDLECGRTYENLKEAVNKGLLTETDIDVAVKRLFTA
                     RMKLGMFDPEGKNPYDTIPFSVNDNEKHRELSLKVARETMTLLKNGNNVLPLSKDIKS
                     IAVLGPNSNDPVVMYANYNGYPSKYVTPLEGIKQKLGENVKVFHHDVSSLVNDNPIPL
                     PITRENMMTEGV"
BASE COUNT          515 a          335 c          333 g          388 t
ORIGIN      
        1 acagattcat gaagaaggta aaaaaaaagc caatggcaat attttatacg caaatttgca
       61 tcaaatatac ctgcatttgc aacgaataag catgctgaaa tatgtttaat ttcagtattt
      121 ttaatacaat ttgacaagat aagatgcata acgaatcaat caaaaaataa caaatacatt
      181 taaagatgaa gcaaatcaaa tcattaactg catttttcct cgtggttatt tcgatttttc
      241 tgtctattgg atgcgagaaa accgaaaagt ggaagaatcc taaccttccg gttgaaaaac
      301 gtgtaaacga ccttgtatca agaatgaccc ttgaagaaaa gatttcgcaa atgatggacg
      361 tggctgcccc cattgaacga ttgggaattc cggcatacaa ctggtggaat gaagggcttc
      421 acggagttgc ccgagctggt attgcaacgg tattcccgca agccattggt gttgctgcca
      481 cctttaacga tagcctttta cttgttgaag cacaggtaat ttcagatgaa ttcagggcaa
      541 gatataacaa ttatatcaag aaaaatgaac gcgaccgcta caaagggtta actatctggt
      601 ctccaaatat taacatcttc agagacccac gttggggacg cgggcaagaa acctatggag
      661 aagatccata cctcacatca cgactgggtg tagcctttgt gaaaggcctg cagggcaaca
      721 accccaacta tcttaaaaca attgctaccc ctaagcacta cgtggtacac agcggaccag
      781 aaaaagaccg tcacgttttt gatgccgacg taacagaacg agatttcatg gatacatatc
      841 tccctgcttt tgaagcaacg attaaagaag gaaaagcata ttctatcatg ggtgcttaca
      901 atcgttttcg aggtgaatcg tgtagtggca gcgacacatt gcttaatcag atcttgcgaa
      961 aacaatgggg cttcaaaggg tatgtcgtct cagactgtgg tgccatcgat gatatttatc
     1021 ttaaccataa aattgtacca acaggtgccg aagctgctgc aattggagtg cttggcggct
     1081 gcgacctcga atgcgggaga acatacgaga acctgaaaga agccgtaaat aaaggtcttc
     1141 tcactgaaac cgacatcgac gtagcagtga agcgactttt cactgcccga atgaaactgg
     1201 gtatgttcga ccctgaaggg aaaaacccct acgatactat acctttctcg gttaacgaca
     1261 atgaaaaaca ccgggaactc tcgcttaaag tagcccggga aacaatgacc ctgctgaaaa
     1321 atggcaacaa tgtacttcca ctgagcaaag acataaaatc gattgccgtt cttggtccca
     1381 actcgaacga cccggtagtt atgtatgcca attataatgg ttacccatcg aagtatgtta
     1441 ccccgctgga aggaattaaa caaaagttgg gagaaaatgt aaaagtgttc caccacgatg
     1501 tttcctctct ggtaaacgat aatcccatcc ccttgcccat cacccgtgaa aacatgatga
     1561 cagaaggagt t
//
LOCUS       DAOPUW010000527         1570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_89248_length_1570_cov_3.599340, whole genome shotgun sequence.
ACCESSION   DAOPUW010000527 DAOPUW010000000
VERSION     DAOPUW010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89248_length_1570_cov_3.599340"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..292
                     /locus_tag="PKJ43_08355"
     CDS             8..292
                     /locus_tag="PKJ43_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016779071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNZ72619.1"
                     /translation="MTKADIVNEISKNTGIEKVTVQKTVEAFMESVKDSLVDGNNVYL
                     RGFGSFIVKKRAEKTARNISKNTTIIIPEHNIPAFKPAKTFVNHVKDKVK"
     gene            527..>1570
                     /locus_tag="PKJ43_08360"
     CDS             527..>1570
                     /locus_tag="PKJ43_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNZ72620.1"
                     /translation="MSSELFIDVSPSEIVIALIEDKHLVELTREKSGAKFAVGDIYLG
                     KVKKIMPGLNAAFVDVGFEKDAFLHYLDMGPQFATLNKFVQNATSRKGKVNAMNKIHL
                     DPDIDKNGKIVNLLKPGQIILVQVAKEPISTKGPRLTSELSIAGRNMVLVPFSDKISV
                     SQKIRTVEERNRLKKLIQSIKPHNYGVIIRTASEGKMVAELDQELQRLVSKWESILPK
                     LKRVSPPGLVLGELSRSTALLRDIYSPDFVNINVNDFSVFEEVSDYIAGIAPEKKKTV
                     KHYDKRLPMFEYFGIDKQIKSMFGKTVTFRNGAYLIIEHTEAFHVIDVNSGNRAKAGN
                     NQETNALEVNLSAC"
BASE COUNT          548 a          281 c          315 g          426 t
ORIGIN      
        1 ttaagcaatg acaaaagcag atattgtaaa cgaaatttca aagaacactg gaattgaaaa
       61 agttacagtt caaaaaactg tagaagcttt tatggaatca gttaaggatt cattagtaga
      121 tggtaacaat gtttacctca gaggttttgg tagtttcatt gtaaagaaaa gagcagaaaa
      181 aactgcacgt aacatctcga aaaacactac cattattatt cctgagcaca atatcccggc
      241 atttaaacct gctaagacat ttgtgaatca cgttaaagac aaagtaaaat aatttgtagt
      301 atgcctagcg gaaaaaaaag gaaaagacat aagatggcta cgcacaagcg taaaaagaga
      361 ttaagaaaga atcgtcataa gaagagaaag taatcttttt tggcgttgcc agcttataaa
      421 aaaatttacg attaaaccta aggtgtacct acagcttagg tttaatcctt tatatgaaaa
      481 tgagttcttt tacatagtta ttaattctcc ctttatttga aaaaatgtga gtagcgaatt
      541 atttattgac gtaagtccct ctgagatcgt cattgcactg atcgaagata agcatctggt
      601 tgagttaacc agggagaaaa gcggcgccaa gttcgcagtc ggcgatattt acctcggaaa
      661 agtaaagaag ataatgcccg gcctgaatgc tgcttttgtt gatgttggtt tcgagaaaga
      721 tgctttctta cattacctcg acatgggacc gcaattcgct actttgaaca agtttgttca
      781 gaatgctaca tctcgtaaag gaaaggtgaa tgccatgaac aaaattcacc ttgatcctga
      841 tattgataaa aatgggaaaa tagtgaacct tttaaaacca ggacaaataa ttcttgttca
      901 ggttgccaaa gagccaatct caacgaaagg tccacgctta acatccgaac tatcaatagc
      961 aggtcgaaat atggtattgg ttccgtttag cgataagatt tcggtttctc aaaaaattcg
     1021 tactgttgag gaacgaaacc gcttgaaaaa acttattcaa agtattaaac cacacaatta
     1081 cggagttatt atcagaacgg cctctgaagg aaaaatggtg gccgaactcg atcaggagtt
     1141 acaacgtctc gtcagcaagt gggaatcaat ccttcccaaa ttaaaaagag tttctccccc
     1201 tggcctcgtg ctgggagagc tcagtcgctc aaccgctttg ctgagagata tatacagtcc
     1261 tgattttgta aatataaacg ttaacgattt ttctgttttc gaagaagtaa gcgattacat
     1321 tgcgggaatt gcacccgaga aaaagaaaac cgtaaaacat tacgacaaaa ggctcccgat
     1381 gttcgaatat ttcggaatcg ataagcaaat caaatccatg ttcggcaaaa cagtcacgtt
     1441 tcgaaatggt gcttatctga ttatcgaaca taccgaagca ttccatgtaa ttgatgtgaa
     1501 tagcggaaac cgtgctaaag ccggtaataa tcaggaaaca aatgctctcg aagttaatct
     1561 gtcggcttgc
//
LOCUS       DAOPUW010000528         1570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_89258_length_1570_cov_2.982178, whole genome shotgun sequence.
ACCESSION   DAOPUW010000528 DAOPUW010000000
VERSION     DAOPUW010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89258_length_1570_cov_2.982178"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PKJ43_08365"
     CDS             complement(<1..421)
                     /locus_tag="PKJ43_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HNZ72621.1"
                     /translation="MNNIFVYCEIEDGIIADVSLELLTKGRSLADQLACKLEAVVIGS
                     KLDEIGKQVFPYGVDVLHVADDPRLAPYTTLPHTSIIVNLFKAEKPQIALMGASPVGR
                     DLGPRVSSALYSGLTADCTSLEIGGHEDKKENKVYENL"
     gene            complement(468..1343)
                     /locus_tag="PKJ43_08370"
     CDS             complement(468..1343)
                     /locus_tag="PKJ43_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNZ72622.1"
                     /translation="MKAFKIIVLAKQVPDTRNVGKDAMKADGTVNRAALPAIFNPEDL
                     NALEQALRIKDQFPGTTITILTMGPGRAADIIREGMFRGADDGVLLTDRAFAGSDTLA
                     TSYALSRAIAKIKQYDLIICGRQAIDGDTAQVGPQVAEKLNMPQVTYVEEIIQADNKS
                     IQMKRRLDNGVEIVKAPIPVLLTVTSSASACRARNAKFLMKYKHAKTISERQSENEDY
                     MSLYHDRPYLNIPEWSVNDVEVDPQQLGLSGSPTKVKKIDNVVLQAKEAKKIDASDAE
                     INLLMKELIDSHTIG"
BASE COUNT          392 a          354 c          329 g          495 t
ORIGIN      
        1 acaggttttc gtacacttta ttttcttttt tgtcttcgtg cccgccaatt tcaaggctgg
       61 tacagtcggc tgtcaaaccg ctatacaatg cagaagaaac gcgtggaccg aggtcccttc
      121 ctacaggact tgcccccatt aaagcgattt gaggtttttc ggctttaaag agatttacaa
      181 taattgacgt gtgagggagt gtagtatacg gagccagtct tgggtcgtcg gcaacatgca
      241 atacatcaac accgtatggg aatacttgtt tcccgatttc atcgagtttt gacccgataa
      301 ctacagcttc gagcttacaa gcaagctggt cggcaagcga gcgccctttg gtaagcaatt
      361 cgaggctcac atcggcaatt attccatcct ctatttcgca ataaacaaat atgttattca
      421 tttcatttta gactttagat tttaaacaag cgtaaacaaa tcacgtatta accaattgta
      481 tgactatcga ttagttcttt catcaacaag ttgatttcgg catcgctggc atcgatcttt
      541 ttagcttctt ttgcctgaag taccacattg tcgatttttt taacttttgt aggagaacct
      601 gaaagcccca attgctgagg atcgacttcg acatcgttca ccgaccattc tggaatattc
      661 aggtaaggac ggtcgtggta aaggctcata tagtcttcat tttcactctg ccgctcgctg
      721 atagtttttg catgtttgta tttcatgaga aatttggcat ttcgtgcccg gcaagcagat
      781 gccgaactgg taactgtcag cagcactggt ataggtgcct taacgatctc taccccgtta
      841 tcgaggcgac gtttcatttg tatgcttttg ttatcggctt gaattatttc ttcgacataa
      901 gtaacctgcg gcatattgag tttttcggca acctgtgggc ctacctgagc agtatctcca
      961 tcgatggctt gcctgccgca aataatcaaa tcgtattgct taattttagc aattgcacgc
     1021 gataaggcat aggaagttgc cagggtatcg gaaccagcaa aagcgcggtc ggtaagcaac
     1081 acaccatcgt cggcaccacg gaacatacct tcgcgaataa tatcggcagc cctgcctggg
     1141 cccatggtta aaatggtaat agttgttccc gggaactggt ctttaatgcg gagagcttgt
     1201 tctaacgcgt taaggtcttc cgggttaaaa attgccggga gggcagccct gtttacggtc
     1261 ccatcggctt tcatcgcatc tttccctaca tttctagtgt cgggaacctg ttttgccaaa
     1321 acaatgattt taaatgcttt cattcgtatt catttcattt tagattattt actagctgtt
     1381 agattcgaag gttgtcggtt tattttcgtc cctcaagtga accctgcaac ataatacgcc
     1441 tcatttgact acaaccaagt ttattcattt cctgcggtct tgcttttgat tattccgcta
     1501 cacttactca tcgctaaaaa acaatgcgca atttgttccc agatgaaatg atttggtttt
     1561 gccaaaaatg
//
LOCUS       DAOPUW010000529         1570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_89265_length_1570_cov_2.878548, whole genome shotgun sequence.
ACCESSION   DAOPUW010000529 DAOPUW010000000
VERSION     DAOPUW010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89265_length_1570_cov_2.878548"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PKJ43_08375"
     CDS             complement(<1..477)
                     /locus_tag="PKJ43_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72623.1"
                     /translation="MKRNKIIATIILTIASSGFTFAQDTIMNKEVEVIKAFQPTISES
                     NKIVSNPKINDTVKYTPIFDYRIQSSLVPVPRSIQKLPVVQLGNPPQSHSNTGYLRAG
                     FGNAYTPFAEFALNTSPAKATDFGIFFSHFSSSPKLKLNNRIRVKAPYSDDVATIFV"
     gene            complement(543..>1570)
                     /locus_tag="PKJ43_08380"
     CDS             complement(543..>1570)
                     /locus_tag="PKJ43_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ72624.1"
                     /translation="NETSLTYYKKVVDNYPNTEESKAALVGIKNNYIDMNNVDAYFDY
                     SGTAGGKQGQVSTSAQDSIFYMAAERNYMAGESGAEAKFDEYLTRFPNGSFKLNASFY
                     LAEICYSKGSYSRSLELYETVASMGDNIFSEPALLKAGELSFNAQKFDASLDYFQRLG
                     KVATTKWNILKSRAGIMRSYFKLSDYANSITAAQLVMKTENVTEAITREANYIQATSY
                     YKQSQPDEALKFYTLLANDTKLAEGAEAKYNKAKILFEKNLLDQSEAEILDFINKNTP
                     HQYWLAQSFILLTDIHLARKDVFQARHTLQSLIDNYTNQTDGVLTLAKEKMQVVESLG
                     NQNIIEE"
BASE COUNT          430 a          297 c          314 g          529 t
ORIGIN      
        1 aacaaaaatc gtagccacgt cgtcagaata aggagcctta acccgaattc tgttattgag
       61 ttttaatttc ggacttgatg aaaaatgaga aaagaaaata ccaaagtcgg tcgctttagc
      121 cggcgaagta ttcaatgcaa attcggcaaa tggggtatag gcatttccaa aaccagcccg
      181 taaataccca gtattactgt ggctctgcgg cggatttcca agttgaacta ccggcaattt
      241 ttgaatagag cgtggtactg gaaccaacga tgattgtatg cggtaatcaa aaattggggt
      301 atatttcacg gtatcgttaa tcttgggatt cgatacaatt ttatttgatt cacttatggt
      361 tggctgaaat gctttaatta cttcaacctc tttgttcata atggtatcct gggcaaatgt
      421 aaaaccgctg ctggcgattg ttaatattat agttgctatt attttgttcc gtttcatctg
      481 tatcttttcg ttattagatt tttgactttc caacttcttc gtggcattaa tgcaatcaca
      541 agctattctt caatgatatt ttgatttcca agactttcaa caacttgcat cttttcttta
      601 gctaatgtca acactccatc ggtttggtta gtatagttgt ctataagact ttgcaatgta
      661 tgtcgggcct gaaatacgtc ttttctggca agatgaatat cggtgaggag gatgaacgat
      721 tgagctaacc aatactgatg aggtgtattc ttgttaataa agtcaagaat ttctgcctcc
      781 gactgatcga gaaggttttt ctcgaaaagt atttttgctt tgttgtattt agcttctgca
      841 ccttcggcaa gctttgtgtc gtttgccaat aaggtataaa acttcagcgc ttcgtcgggt
      901 tgcgactgtt tgtaatacga agttgcctgt atataattgg cttcacgtgt tattgcctcg
      961 gtaacatttt cggttttcat taccaattgt gcagcagtaa ttgagttggc ataatcagac
     1021 agtttgaaat agctacgcat aatgccagct ctcgatttta ggatattcca tttggtagta
     1081 gctactttgc caaggcgctg gaaataatca agcgatgcat cgaatttttg agcattaaat
     1141 gacaattccc ctgcttttaa caaggctggt tcactaaaaa tattatcgcc cattgaagct
     1201 acggtttcat ataattcgag tgaacgggaa tacgatcctt tgctgtaaca aatctcagcc
     1261 agataaaaag aggcattcaa tttgaaactg ccattgggga aacgggtaag atattcatca
     1321 aattttgctt ctgcgccact ttcgccagcc atatagttcc gttcggcagc catataaaat
     1381 attgaatctt gggcagatgt tgacacttgc ccctgcttac ctccagccgt tcccgaataa
     1441 tcgaaataag cgtctacatt gttcatgtcg atatagttgt tcttaattcc aaccaaagcg
     1501 gctttcgatt cttcagtatt tggataattg tcgacaacct ttttataata tgtcagcgat
     1561 gtttcgtttt
//
LOCUS       DAOPUW010000530         1569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_89333_length_1569_cov_5.373184, whole genome shotgun sequence.
ACCESSION   DAOPUW010000530 DAOPUW010000000
VERSION     DAOPUW010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89333_length_1569_cov_5.373184"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..753)
                     /locus_tag="PKJ43_08385"
     CDS             complement(1..753)
                     /locus_tag="PKJ43_08385"
                     /inference="COORDINATES: protein motif:HMM:NF020118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HNZ72625.1"
                     /translation="MDNVNLIDTFAEFKDLKNIDRATMMSVLEDVFRNLLQKKYESDE
                     NFDIIINIDKGDFEIWHYRTVVADEEFSDPNKEIPLSEAKKIDQDYEVGEEVSDEVKL
                     IDFGRRAILNLRQNLASRILELEKDGIYTKYKDKIGEIVTGEVYQIWKKEILVLDDEG
                     NELILPKTEQIPSDYFRKGDSIRAVVYKVELRNNTPFIVLSRTQPAFLERLFELEVPE
                     IFDGLITIKKIVRVPASGQKLPLNRTTNVSTR"
     gene            complement(766..1230)
                     /gene="rimP"
                     /locus_tag="PKJ43_08390"
     CDS             complement(766..1230)
                     /gene="rimP"
                     /locus_tag="PKJ43_08390"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HNZ72626.1"
                     /translation="MIKKEIIEQLTADVLTGEFFLIQVTVKQGNVIEVIIDGDNGVSI
                     QKCVDVSRYIEQHLDRESEDFELSVYSAGLGNPFKVYRQYVKNIGKEVDVKLGEEKPL
                     SGTILSVDENGFDLETKVSIKDEATRKKKEVKKVVRFNFTDQPEVKNSITFK"
BASE COUNT          403 a          344 c          278 g          544 t
ORIGIN      
        1 ctaccgggtc gatacgttcg tcgtacgatt caacggcaac ttttgcccgc tggccggaac
       61 ccttacaatt ttcttaatgg ttatcaagcc atcaaaaatt tcgggtactt caagctcgaa
      121 aagcctttcg aggaaggccg gctgtgtacg cgaaagaaca atgaatgggg tgttgttcct
      181 cagttctacc ttataaacaa ctgcccgaat gctatcgcct ttgcggaaat aatcgctggg
      241 tatttgttct gtttttggga gaatcagttc gttgccttca tcatcgagta ccaaaatttc
      301 ttttttccag atttggtata cttctccggt aacgatttca ccaattttgt ctttgtattt
      361 ggtatatatt ccgtcttttt ccagttcaag gattcggctt gcgaggttct gccgtaggtt
      421 caggattgcc ctgcgcccga aatcgatcag tttaacttcg tcgctaactt cttcaccaac
      481 ttcataatcc tggtcaattt ttttggcttc cgaaagtggt atttctttgt tagggtcgga
      541 aaattcttcg tcggcaacta ctgtacggta atgccagatt tcgaagtcgc ctttgtcgat
      601 gttgataatt atgtcgaagt tttcgtcgct ttcgtacttt ttttgaagca gattgcggaa
      661 aacatcttca agaacgctca tcatcgttgc cctgtcgatg ttctttaaat ccttaaattc
      721 ggcaaacgta tcgataagat tgacgttgtc cattgtctgt ttttattatt taaatgttat
      781 actatttttt acttccggct gatcggtaaa attgaaccgg actactttct tcacttcttt
      841 ctttttccgg gtggcctcat ccttaatgct tacttttgtt tcaaggtcga acccgttttc
      901 atcaacacta agtatggttc cgcttaacgg tttttcttct cccagcttaa cgtcgacttc
      961 ctttccaatg tttttcacat attgcctgta aaccttaaac ggatttccca gccctgctga
     1021 atatacactc agctcaaaat cttcgctttc gcgatcgaga tgttgttcaa tataccggct
     1081 gacgtcaacg catttctgta tggaaacccc gttatcgcca tcgataatta cttcaatgac
     1141 atttccctgt tttacggtaa cctggattaa aaaaaattcc ccggttaaaa catctgcggt
     1201 taattgctca attatctcct tcttaatcat tatgaacagg taataaaata ggggaccacc
     1261 cgtcccctat aatttcactt acagaatcgg tgcaaaaata caaataataa tgaatcaaac
     1321 aaaatcagca agttaattat caatacttct cgttataatt ttttttgaat cgaaaactaa
     1381 tcatggtttt tatgtgttac tttggcttaa acttaggaat ataaaaccta tgaattcgac
     1441 gaccaacaag cgtaaaatta tcaatgaccc ggtgttcggg ttcatcaact ttccttcgga
     1501 aacaattttt gatttggttg aacaccctta tttacagcga ttaaggcgca ttaaacaact
     1561 tggtctttc
//
LOCUS       DAOPUW010000531         1569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_89346_length_1569_cov_3.632761, whole genome shotgun sequence.
ACCESSION   DAOPUW010000531 DAOPUW010000000
VERSION     DAOPUW010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89346_length_1569_cov_3.632761"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PKJ43_08395"
     CDS             complement(<1..732)
                     /locus_tag="PKJ43_08395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ72627.1"
                     /translation="MKLSVVIVNYNVKHFLEQCLHSVEKASKNLATEVFVVDNNSVDG
                     SKGMLLSKFPNIKLVLNEKNVGFSRANNQAIRMATGQYVLLLNPDTVVEEDTFEKVVA
                     FMDSHPEAGGLGVKMIDGKGKFLPESKRGLPTPWVAFYKVFGLSAIFPHSKVFGKYHL
                     SYLDKNELHQVDVLAGAFMLLRKEALDKVGLLDEGFFMYGEDIDLSYRITLGGYKNYY
                     YPDTTIIHYKGESTKKGSLNYVKMFY"
     gene            complement(793..1419)
                     /gene="recR"
                     /locus_tag="PKJ43_08400"
     CDS             complement(793..1419)
                     /gene="recR"
                     /locus_tag="PKJ43_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNZ72628.1"
                     /translation="MLHERLPSRLLENAVEEFSKLPGIGRKTALRLVLYLLRCEESEV
                     EDFGNAIITLRKEVKHCEICHNISDNEVCPVCSNPSRDASVICVVENIRDVMSIENTN
                     QFRGLYHVLGGVISPMDGVGPGNLEIVSLIDRASKGDIQEVILALSTTMEGDTTNFYI
                     YKKLKDTGIKITTIARGVSVGGELEYTDEITLGRSIINRLDFTSTFNQ"
BASE COUNT          466 a          360 c          283 g          460 t
ORIGIN      
        1 atagaacatt ttcacatagt taagactgcc ttttttggtg ctttctcctt tataatgaat
       61 aatagttgta tcgggataat agtaattttt gtacccgcca agggtaattc ggtacgacaa
      121 gtcgatatct tcgccataca taaaaaagcc ttcatcgagc agcccaacct tatcgagcgc
      181 ttctttgcgc aaaagcataa atgcgccagc cagcacatct acctgatgca attcattttt
      241 atcgagatat gataaatggt atttcccaaa aactttcgaa tgcgggaaaa ttgcagacag
      301 gccaaaaacc ttgtaaaaag ctacccatgg ggttggcaac ccacgtttcg attcgggcag
      361 aaattttccc tttccatcga tcattttaac tccaagtccg ccggcttcag ggtgcgaatc
      421 cataaaagcc accacttttt cgaacgtatc ttcttctaca acagtatcgg gatttaagag
      481 caaaacgtat tggcctgttg ccatacggat agcctgattg tttgcccttg agaaacctac
      541 atttttttcg ttgagaacga gctttatatt ggggaatttg ctcagcaaca tgcctttcga
      601 tccatcgaca gagttattgt cgacaacaaa aacctcggta gcaagattct ttgaggcctt
      661 ttcgaccgag tgcaaacact gctcgagaaa gtgcttcaca ttatagttta cgataacaac
      721 cgaaagtttc atgcatttag cttttataaa actgaatatt gctggtaaat tacatcattt
      781 ctttgtcttt tcttactggt tgaatgttga tgtaaaatcc aaccggttta ttattgagcg
      841 gcccagggtt atttcatcgg tgtattcaag ctcgcccccc accgaaacac cccgggcaat
      901 ggtggtaatt tttattccag tatctttcaa tttcttgtaa atataaaaat ttgttgtatc
      961 gccttccata gtggtactta gggctaaaat tacttcttga atatcccctt tgctggcacg
     1021 atcgataagc gaaactattt cgaggtttcc tggccccacc ccatccattg gcgaaattac
     1081 accgcccaac acatggtaaa gccccctgaa ttggttggta ttctcgatcg acatcacatc
     1141 gcgaatattt tctaccacac aaattactga agcatcacga gaagggtttg aacacaccgg
     1201 gcaaacctca ttatctgaaa tattgtggca aatttcgcaa tgtttaactt ctttcctcag
     1261 ggtaattatc gcatttccaa agtcttcaac ctcgctctct tcgcaacgca atagatataa
     1321 taccagacgc aaagctgttt tgcgaccaat ccccggaagt ttcgagaact cctcaacagc
     1381 attctccagc aagcgtgatg gcaaacgttc gtgcaacata attcaatata aatggcaatc
     1441 aaaggaaaaa aataaagcag agaaaaacaa aagccgggag aaattttgac agtattctcc
     1501 aataataccg attaaagaaa tgtaacagtc caatcttcgc ccactcgatt gtactgaaca
     1561 tttctattc
//
LOCUS       DAOPUW010000532         1569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_89362_length_1569_cov_3.119551, whole genome shotgun sequence.
ACCESSION   DAOPUW010000532 DAOPUW010000000
VERSION     DAOPUW010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89362_length_1569_cov_3.119551"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PKJ43_08405"
     CDS             complement(<1..710)
                     /locus_tag="PKJ43_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNZ72629.1"
                     /translation="MGTSGINSFYSANSIPEYAFCTPGTLTGKNSLHRIIPPSNITLI
                     GKYAFYGCRNIKNFTMSPLVSQIENFAFNGCSNLTSFVTNSSLTSLGAYSFSGCKNLV
                     SFQFPTSLVSIGGNAFENCIKLTSIEIPASITSMGSAVFKGCSGLQSATVNASIPELG
                     MKLFYGCSKLTSFAIPTTVKSIGENAFGYCSNLNTISIPSTATSIKQEAFSYCINLQN
                     VTLPESLTYIGTYAFEMCT"
     gene            complement(738..1226)
                     /locus_tag="PKJ43_08410"
     CDS             complement(738..1226)
                     /locus_tag="PKJ43_08410"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HNZ72630.1"
                     /translation="MTKDIYAAANQWCDFSTIVDRSGFYLSSSRADVFAEEGNRDSIV
                     IFADVPWTISSIPSWLTVFPENGTGNDTLVFTANANTEYTDRRANLTVSASGIPSQTI
                     EFIQWGLPKTVTVTAGNLSQEISSLEKNSISNLKLIGTINACDFKTMRDSMPVLSVVD
                     LS"
     gene            1375..>1569
                     /locus_tag="PKJ43_08415"
     CDS             1375..>1569
                     /locus_tag="PKJ43_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72631.1"
                     /translation="MEGMVIDVRFEQLSNALSPILVRVEGKDIEASSTQLIKAALFIV
                     VNVEGNTTETKSLQLKKVSSS"
BASE COUNT          482 a          285 c          341 g          461 t
ORIGIN      
        1 gtacacattt caaaagcata agtgccaata tatgttaacg attcagggag tgtgacattt
       61 tggagattta tacaataact aaatgcttct tgtttgattg atgttgcagt tgagggtatg
      121 gatatggtat tcaggttgct acaatatcca aaagcatttt caccaattga tttcactgtt
      181 gttggaattg caaatgaggt taatttgctg cagccataga acaatttcat cccgagctca
      241 ggaattgacg cgttaaccgt tgccgattgc aacccggagc aacctttgaa cacggcactg
      301 cccatagaag ttattgatgc cggtatttca atagaagtta gtttgatgca attctcaaag
      361 gcattgcctc cgatagatac taacgaggtt ggaaactgga acgagaccag atttttacat
      421 cctgaaaatg aataagcccc gagcgaagta agcgaagagt ttgtaacaaa cgaagtgagg
      481 ttgctgcatc cattaaaagc aaaattttcg atttggctta ctaatggact cattgtgaaa
      541 tttttaatat ttcgacaacc gtaaaaagca tacttcccta ttaaggtaat gtttgaaggg
      601 ggtattatcc tgtgtaaact gttttttcca gtcaaagtgc cgggagtaca gaaggcatat
      661 tcaggaatac tgtttgcaga atagaacgaa ttaattccgc ttgtacccat tgttcctgag
      721 tattgaacaa tgctgaccta gctcaaatcc accaccgaaa gtacgggcat gctgtcgcgc
      781 atagtcttga aatcgcaagc gttaatggtc ccaattaatt ttaggttcga tatactgttc
      841 ttttctaatg agctaatttc ttgagagagg tttcctgccg ttactgttac tgttttgggt
      901 aagccccatt gaatgaactc aattgtctgc gagggtattc ctgacgccga aactgtaagg
      961 ttggcccttc ggtcggtata ttctgtattg gcattggcag taaaaaccag cgtgtcgttg
     1021 ccggttccat tttcaggaaa aacagttaac catgatggaa ttgaactgat tgtccagggc
     1081 acatcagcga agatcacgat agaatcacga ttcccttcct ctgcgaaaac atcggcacgt
     1141 gacgaggata aataaaaacc agagcgatca acgatggttg aaaaatcgca ccattggttc
     1201 gctgcggcat aaatatcttt tgtcatataa ggcacataca atgtacaaga agtgttgtca
     1261 acattataaa aaacctcagg cgacaaactt aaatcgatgg gattgggcga atcaacgtaa
     1321 atggatgtta aatttgtaca gtatctaaaa gcttgtttgt taattgtttg tatcgtggag
     1381 ggaatggtta tcgacgtaag attcgagcaa ttgtcaaacg cattatcacc aatacttgta
     1441 agggttgaag ggaaggatat agaagcaagt tcaacgcaat tgataaaagc agctcttttt
     1501 attgtggtta atgttgaagg taataccact gaaaccaaat ctctgcaatt gaaaaaagtt
     1561 tcctcttca
//
LOCUS       DAOPUW010000533         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_89458_length_1568_cov_2.884336, whole genome shotgun sequence.
ACCESSION   DAOPUW010000533 DAOPUW010000000
VERSION     DAOPUW010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89458_length_1568_cov_2.884336"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PKJ43_08420"
     CDS             complement(<1..993)
                     /locus_tag="PKJ43_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72632.1"
                     /translation="MTEPQSVSINQTVSIFPDYAGVTVPANIAPLNFYIEEKGIEYQA
                     NFVDASGNGFVVKSDNGVVDIPLKKWHKMLASNAGKSINVKIFVKDNSGKWAAYQPFS
                     IAVSSDNIDSHLAYRIINVGYILWNKLGLYQRDLTSFRQTAIMLNRNTDGNCMNCHNF
                     NQQNPEQMMFHMRGKYAGTVIARGDEVKKVNTKTPVTMSAAAYPSWHPGGKHIAFSVN
                     LISQWFHGVDKKNEVYDRASDIVVYDIENNEITTSPLVSTRQRESLPNWSPDGKYIYF
                     CRTEEVSESIAWDEVAYDLCRISYDVETKTWGTVETVLSAAQAGGSITFPKISPD"
     gene            complement(1065..>1568)
                     /locus_tag="PKJ43_08425"
     CDS             complement(1065..>1568)
                     /locus_tag="PKJ43_08425"
                     /inference="COORDINATES: protein motif:HMM:NF039640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6057 family protein"
                     /protein_id="HNZ72633.1"
                     /translation="RMYEKHLNGTTNPDLEDLIALKRELMPFTDFKVNRQEPSEDLIN
                     ILIDKPKNRMAYEYLMSYFLLNNDLASFVKYYSIGRNFSYGKVPKVYQQALLLYSYEL
                     GRVGRPIGNLRIEKEIVQQFNEYLSIIAQNQGNMDAALPELSEKFGQTYWFYIHYNSP
                     VTTTDKQ"
BASE COUNT          445 a          324 c          312 g          487 t
ORIGIN      
        1 atcgggagaa atctttggaa aagttatgct tcctccagct tgtgcagctg aaagtacggt
       61 ttcaaccgtt ccccaagttt ttgtttcaac atcgtaagag atacgacaca gatcgtaagc
      121 tacttcatcc caggcaattg attcagaaac ctcttctgtt cggcaaaaat atatgtattt
      181 cccatcgggc gaccaattag gcagtgattc cctctgtcgt gtagaaacaa gtggagaagt
      241 cgtaatttca ttattttcaa tatcgtacac tacaatatca gaagctcggt cgtacacttc
      301 atttttctta tcgaccccat gaaaccactg actgataagg tttaccgaaa aagcaatatg
      361 cttacctccg ggatgccacg atggataagc agctgccgac atggtgaccg gtgttttggt
      421 gttcactttt ttcacttcat cgccacgggc tataactgtt cctgcatatt ttccccgcat
      481 gtgaaacatc atctgttcag ggttttgctg gttgaagtta tgacagttca tgcaatttcc
      541 atcggtattt cggtttagca ttatggccgt ttgcctgaat gaagttaagt cgcgctgata
      601 caaccccaac ttattccaaa ggatataacc tacattaata atacggtatg ccagatgcga
      661 atcgatatta tcggaagaaa cagcaataga aaatggctga taagcagccc atttcccgga
      721 attatctttt acaaagatct ttacattgat tgattttcca gcgttcgagg ccagcatttt
      781 atgccatttt ttcaagggaa tgtcaacaac cccgttgtct gatttcacaa caaaaccatt
      841 cccgctggca tcaacaaaat ttgcctggta ttcaattccc ttttcttcta tgtaaaaatt
      901 gagtggtgca atattggcag gcaccgttac tcctgcataa tcggggaaaa ttgacacggt
      961 ttgattaatg cttacgcttt gaggctctgt catctttgta gagcaagaag caaagatgac
     1021 gactatcaat atgtatagta gatgttgtat tgtcttcata gagcttactg tttatcggtg
     1081 gtggtaacag ggctgttata atgaatgtag aaccaatagg tctggccaaa cttctcactt
     1141 aattcgggca aagcggcatc catgttccct tggttttggg cgataattga aagatattca
     1201 ttgaattgct gaacgatttc tttctcgatc cgaaggttgc cgataggacg cccaactcgg
     1261 cctaactcgt aagaatacaa caaaagggct tgttgataga ctttgggaac cttcccataa
     1321 ctaaaattcc gacctataga ataatatttt acaaacgagg ctaaatcgtt gttcaataag
     1381 aaataagaca tcagatattc gtaagccatc ctgtttttgg gcttatctat caggatatta
     1441 ataagatctt ccgatggttc ctgacgatta actttgaaat cggtaaatgg cattaattct
     1501 cttttgaggg caatcaaatc ttcaagatcg gggtttgtag tcccatttaa atgtttctcg
     1561 tacatacg
//
LOCUS       DAOPUW010000534         1563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_89976_length_1563_cov_1.966180, whole genome shotgun sequence.
ACCESSION   DAOPUW010000534 DAOPUW010000000
VERSION     DAOPUW010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89976_length_1563_cov_1.966180"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PKJ43_08430"
     CDS             <1..405
                     /locus_tag="PKJ43_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HNZ72634.1"
                     /translation="TLRLEMGYVLYGNDINESTSPLEAGLGWITKFVDHKNFIDKEYM
                     LNLKTAGLKRKLVGFEMVDRGIPRQHYEIFDSQGNLIGEVTSGTMSPMMKIGVGMGYV
                     GTEFSKPDTEIFIGVRDKKLKAKVVKLPIYKK"
     gene            640..>1563
                     /gene="serC"
                     /locus_tag="PKJ43_08435"
     CDS             640..>1563
                     /gene="serC"
                     /locus_tag="PKJ43_08435"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNZ72635.1"
                     /translation="MKKHNFNAGPCVLPKPAIEATIEAIRDFDNTGIGLLEISHRTPG
                     WEKVMAETEQLWRELLNIPEEYAVIFLGGGASTQFYTLAANFLNKKAAYLETGVWAKK
                     AIKEAKLYGEVEVVGSSADKNFTYIPKGYTIPTDVDYFHITTNNTIYGTEIKTDIDSP
                     VNLIADMSSDIMSRPVDIKKYAMIYGGAQKNVGPAGVTFVIVRKDLLAKVQRKLPSMV
                     DYKTHIGAEPKDNSMFNTPPVISVFVMNQTLKWVKSLGGVKEMQKLNQKKAELLYNEI
                     DRNKMFVGTAVKEDRSMMNVCFVMAEQYKDKE"
BASE COUNT          490 a          309 c          343 g          421 t
ORIGIN      
        1 accctgaggc ttgagatggg ctacgtgctt tacggaaatg atataaacga gagcacatct
       61 ccactggaag caggattggg ttggattacc aagtttgttg accataaaaa ttttattgac
      121 aaggagtata tgctcaacct taaaactgcc ggattaaaac gaaaacttgt tggctttgag
      181 atggttgaca gaggtattcc ccgtcagcat tacgaaatat tcgatagcca gggaaacctt
      241 attggcgaag taacctcggg aaccatgtcg cccatgatga agattggcgt tggaatgggc
      301 tacgtcggta ctgagtttag caaacccgat acggaaattt tcattggcgt tagggacaaa
      361 aagctgaagg ccaaagtggt gaaactgcct atctataaaa agtagctttg agattgtctt
      421 ttctctgggg ggcatgaacc taatcatgcc ccattttttt tgctaaaaaa atcattatcc
      481 atttttcaat cttcagtaaa taatgccctc aatccgctat ggaatgtagt ttttacttcc
      541 tgctttaaat catttcagat ttttaggaaa tgtgaaaaat aagttggaaa tttggcagca
      601 attaccacta ttgtcttaca atttaaaact ttatacgcta tgaaaaaaca caactttaat
      661 gcgggaccct gtgtccttcc aaaaccggca atcgaagcta caattgaagc tatccgcgat
      721 tttgacaata ccggaattgg ccttctcgaa atttctcacc gtacacccgg atgggaaaaa
      781 gtaatggcag aaaccgagca gctgtggaga gagttgctta atattcccga ggagtatgct
      841 gttatattcc ttggtggcgg tgcatcaaca caattctaca cccttgcagc aaacttcctg
      901 aacaaaaaag ctgcatacct tgaaaccgga gtttgggcta agaaagccat caaagaggcc
      961 aagctatacg gcgaggttga agtggtgggt tcgtcggccg ataaaaactt tacctatatc
     1021 ccaaagggat acaccatccc aaccgatgtt gactacttcc atatcaccac aaacaatacc
     1081 atttacggaa ctgagataaa aactgatatc gacagcccgg tgaacctgat agccgatatg
     1141 agctctgata ttatgagccg tcctgttgac attaaaaagt atgccatgat ttatggtggt
     1201 gcacaaaaaa atgtgggtcc tgctggtgta acctttgtta ttgtacgtaa agatttactt
     1261 gcaaaagtac aacggaaatt gccttcgatg gttgattaca aaacccatat cggagccgaa
     1321 ccaaaggaca actccatgtt taacaccccg ccggtaatct ccgtttttgt gatgaaccaa
     1381 acattgaaat gggtgaaatc acttggcggt gttaaggaaa tgcagaaact caatcagaaa
     1441 aaagctgagc ttttgtataa cgagatcgat cgtaataaga tgtttgttgg aactgccgtt
     1501 aaggaggatc gctcaatgat gaacgtttgc tttgtaatgg cagagcagta caaggataaa
     1561 gag
//
LOCUS       DAOPUW010000535         1560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_90228_length_1560_cov_2.764120, whole genome shotgun sequence.
ACCESSION   DAOPUW010000535 DAOPUW010000000
VERSION     DAOPUW010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90228_length_1560_cov_2.764120"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PKJ43_08440"
     CDS             complement(<1..372)
                     /locus_tag="PKJ43_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016779155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNZ72636.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEGNEPVVIPNSEGKRTTPSVVAFIE
                     NGERKVGDPAKRQAITNAHKTIYSIKRFMGESFDKLSKEVGRVAYEVVKGDNSTPRVK
                     IDDRLYSPQEISAMVLQKMKKT"
     gene            complement(570..1202)
                     /locus_tag="PKJ43_08445"
     CDS             complement(570..1202)
                     /locus_tag="PKJ43_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72637.1"
                     /translation="MRKCSNNFFQTMQQFSSIAVFSINIIISISYLVLLIKKKIKPAL
                     AMWIFFSLAVSMSLITYLKEGNYGFWDNALNTADLFMAFFVTLAIFIFGDTSTKFNSF
                     DIACLGVVGLIIVFWIITKNHLLTNLGVQLILIIAYFPVIKRMLTANENTESFFIWIL
                     LMIAPFISLISSKGALATVYAVRAIFCTTLLLLLMIRIETQKRKQASAKK"
     gene            complement(1183..>1560)
                     /locus_tag="PKJ43_08450"
     CDS             complement(1183..>1560)
                     /locus_tag="PKJ43_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNZ72638.1"
                     /translation="AALDPIEGSNIAYALHFYAGTHKEWLIEKADKAIEKGLCLFVTE
                     YGTTEANGDGAVYEKEIRMWYDWMDKNHISHCNWSIADKKESSAVLVPGASRKGGWSE
                     KQIKPSGKLVQAELLKKHEEMFK"
BASE COUNT          426 a          324 c          317 g          493 t
ORIGIN      
        1 cgttttcttc attttctgaa gcaccatggc cgaaatttcc tgtggtgaat acaagcggtc
       61 atcaattttc acacgtgggg tgctattgtc tccctttacc acttcgtaag ctacacggcc
      121 aacttcttta gaaagtttgt cgaacgattc acccataaag cgtttgattg aatatatcgt
      181 tttgtgcgca ttggtgatgg cctgacgttt ggcaggatct ccgaccttac gctctccatt
      241 ctcgataaat gccactaccg acggagttgt tctttttcct tcgctgttgg ggattactac
      301 aggttcgttc ccttccatca ccgaaacaca cgaattggtt gttcctaagt cgattccaat
      361 tatttttccc atgatatgta atgtttttaa tttattcttt ctgaattacg gtggcagttt
      421 aacaatgatt atgccatgcc aataaaagtc aattttcatg aaaaaatgac acgtatttgt
      481 gtttcggttg gagcaagctt gtcagtactg tatatcattt tctgacaaat aggcttgata
      541 aaaatgattg ggaacaggtt tccccactct cattttttcg ctgaagcttg ctttcgtttt
      601 tgagtttcaa tacgaatcat taaaaggagc agaagtgtgg tacagaagat tgccctcacc
      661 gcatatacgg tagccaaggc ccctttgctg gatataagcg agataaaagg ggcaatcata
      721 agtaaaatcc agatgaaaaa agactcggta ttttcgttgg ctgtcagcat tcgtttgata
      781 accgggaaat aggcaataat taagattagc tgtacgccaa ggttggtcaa caagtggttc
      841 tttgtgatga tccaaaaaac aataatcagc ccaactacac ctaaacaagc tatatcgaac
      901 gagttgaatt ttgtgcttgt atcgccaaaa ataaaaattg ccagggtcac aaaaaaagcc
      961 ataaagagat cagccgtgtt aagggcattg tcccagaacc cataatttcc ttctttgagg
     1021 tatgtgatca ggctcatcga aacggccaaa ctaaaaaaga tccacatggc tagtgcgggc
     1081 ttgattttct ttttgatcaa cagcaccaga tagctaattg aaatgataat gttgatgctg
     1141 aaaacagcta tggaagaaaa ttgttgcatg gtttggaaga agttatttga acatttcctc
     1201 atgctttttg aggagttcgg cctgaacaag cttacccgat ggttttatct gcttttcgct
     1261 ccatcctcct tttcgtgaag ctccgggcac aagtactgcg gacgactctt ttttgtcagc
     1321 tattgaccaa ttacagtggc ttatatggtt tttatccatc caatcgtacc acatgcgtat
     1381 ttctttttca tatacagcac catcgccatt ggcttctgtt gtgccatact cggtaacaaa
     1441 caagcatagc cctttttcta ttgctttatc ggccttctcg ataagccatt ctttgtgggt
     1501 tccggcatag aaatgaagag cataggcaat atttgagcct tcaattggat cgagggctgc
//
LOCUS       DAOPUW010000536         1558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_90405_length_1558_cov_3.021291, whole genome shotgun sequence.
ACCESSION   DAOPUW010000536 DAOPUW010000000
VERSION     DAOPUW010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90405_length_1558_cov_3.021291"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PKJ43_08455"
     CDS             <1..326
                     /locus_tag="PKJ43_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNZ72639.1"
                     /translation="GTMRLGAYACEVKKGSKLFDAYKSELIYERHRHRYEFNDKYIEN
                     YIKAGFKPVGINPESKLVEAMQLDGHPWFVGVQYHPEYSSTVLKPSPLFVEFVKACKE
                     IRANL"
     gene            374..>1558
                     /gene="yidC"
                     /locus_tag="PKJ43_08460"
     CDS             374..>1558
                     /gene="yidC"
                     /locus_tag="PKJ43_08460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNZ72640.1"
                     /translation="MDRNSTIGLVLIFAVLVIFSYINKPSQKEIEAAKQRRDSIEMAN
                     QLAQKEIETQQAAQLAAQTDQQQSSDSLSVENELRNLYGDFSVSATGEDKLIVLENNL
                     MKVSLSTKGGKVQSVELKNYKRHDGSKLILIEDGKSEQHLNFFAQNRNIRTDELYFKP
                     LTSEQNISVSGPSVPKGKEGKPRFNEKEKGESKEITLRLDAGNGRYIDYVYSISHNSY
                     VIGYDIKLNGMERIISTNTGFIDFNFNFDIPRQEKASTYGEDRYTTIFYKFKDDEVDH
                     LKTNKSDSEDLTTPVKWIGYKQLFFVSALIADESFANAAISTDKKDAKSDYVSSVKTE
                     IGLPFETKTSQNYGMSFYFGPNHYQTLKEHGQDMEKLIDLGWPIVREVNRYLIIPVFN
                     FLR"
BASE COUNT          546 a          309 c          305 g          398 t
ORIGIN      
        1 gcggtaccat gcgattgggt gcttacgcct gcgaagtgaa aaaagggagc aagcttttcg
       61 atgcctataa aagtgaatta atttacgaaa gacataggca tcgatacgaa tttaacgata
      121 aatacataga aaattacata aaagcaggct tcaaaccagt gggtatcaac cctgagtcga
      181 aacttgtaga agccatgcaa ttagacggcc atccgtggtt tgttggggta caataccatc
      241 ctgaatatag cagtactgtt ttaaaaccta gtcctctttt tgtagaattt gtaaaagcgt
      301 gcaaagaaat ccgtgcgaac ttgtaatttt gtggaaaatc ttaaaaccaa tattacaaag
      361 aaaaaagtag ttaatggata gaaattcgac aattggactt gtactgatat ttgccgtttt
      421 ggtaatattc agctacatca acaaaccatc gcaaaaagaa atagaagccg ctaaacaaag
      481 acgcgattct attgaaatgg caaatcagtt ggctcaaaag gagatagaga cacaacaagc
      541 agcacaactg gccgctcaaa ccgatcaaca acaatcaagc gacagccttt ctgttgaaaa
      601 tgaattgaga aacctctatg gtgacttttc agtatcggcc actggcgaag acaaacttat
      661 cgtgttggaa aacaacctaa tgaaagttag cctctcaaca aaagggggaa aagttcagtc
      721 ggttgaactt aaaaattaca agcgccacga cggttcaaag ctcatcctta ttgaagacgg
      781 aaaatcggaa caacacctta attttttcgc tcaaaaccgg aatattcgta ccgatgaact
      841 ttattttaaa ccgcttacaa gtgagcaaaa catttcggtt agcggcccct ctgttccaaa
      901 agggaaagaa ggaaaacctc ggttcaacga aaaagaaaaa ggggaaagca aagaaataac
      961 gttacgtctc gatgctggaa atggccgtta cattgattat gtgtattcaa tttcacataa
     1021 ctcatatgta attggatacg acatcaaatt aaatggaatg gaacggatca tttcaaccaa
     1081 taccgggttt attgatttca actttaactt cgatattccc cgtcaggaaa aagcatctac
     1141 ctatggagaa gaccgttata ccaccatatt ttataagttt aaagatgatg aagtcgatca
     1201 tctgaaaact aataaatcag acagcgaaga cttaacaact cccgtgaaat ggattggtta
     1261 caagcaactt ttctttgtat cggctcttat tgccgacgaa tcgtttgcca atgcagccat
     1321 ctcaaccgat aagaaagacg caaaatcgga ttacgtttca tcagttaaaa ccgagatcgg
     1381 tctgccgttt gaaaccaaaa cctcgcagaa ctatggaatg tcattctatt ttgggccaaa
     1441 ccattatcaa accctgaaag aacacgggca agatatggaa aagctcatcg accttggctg
     1501 gccaatcgtt cgcgaagtaa accgatatct tattatcccg gtttttaact ttctcagg
//
LOCUS       DAOPUW010000537         1556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_90597_length_1556_cov_2.648235, whole genome shotgun sequence.
ACCESSION   DAOPUW010000537 DAOPUW010000000
VERSION     DAOPUW010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90597_length_1556_cov_2.648235"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="PKJ43_08465"
     CDS             complement(<1..955)
                     /locus_tag="PKJ43_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNZ72641.1"
                     /translation="MAEQPHSGLYHFEEQQLMPAEEKLEIRKKGQKITIGIPANTDNE
                     EVCIPLTPQAAEMIINSGNDVIIETGAGLKTRYSDLEYSNAGAIIAQSRNEVFECDYV
                     IKVAPFTSEEIALLRGNQVIFSMLQIDKQCESMVKTLMQKKITSVVFEYMKDELGGLP
                     VMQSLSEISGIVAMTVAGELLSNSSGGKGVLFGGVTGISPAAVIILGAETAAESAARA
                     ALGLGAEVKIFDNSVSKLRSFERKFNQKIFTSLYYPRVLKKAIQSADVVLGAQPFNSV
                     PQYIIPREMIQKMKNGSIIIDLNASQGGCFETTTCTSLSNPT"
     gene            complement(1009..1431)
                     /gene="tsaE"
                     /locus_tag="PKJ43_08470"
     CDS             complement(1009..1431)
                     /gene="tsaE"
                     /locus_tag="PKJ43_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNZ72642.1"
                     /translation="MNKTIKIDSLDKIHEAAREFILQMGNATVFAFNGEMGAGKTTFI
                     KAICEELGVEENITSPTFAIVNEYTSNKGLTIFHFDCYRLKNIREAYDIGAEEYFYSG
                     KLCFIEWPEKIEEILPEHCVNIYITVQPNGSRNVDING"
BASE COUNT          419 a          326 c          293 g          518 t
ORIGIN      
        1 atgttggatt cgaaaggctt gtgcatgttg ttgtttcgaa acagccacct tgcgaggcat
       61 ttaggtcaat aattattgac ccgtttttca ttttttgtat catctcgcga gggattatgt
      121 attgtggtac agagttaaag ggttgagccc ccaacacaac atcggcactt tggatagctt
      181 tctttagcac cctgggataa tacagggagg taaatatttt ctggttgaat ttccgttcga
      241 acgagcgaag tttgctaacc gagttatcga aaatcttcac ttcggcacct aatcctaagg
      301 cagctctggc agcagattcg gcagcagttt cggcacctaa aattatgaca gcagctggcg
      361 aaatacccgt gactccacca aaaaggacac cttttccccc tgaactatta ctaagtaatt
      421 ctccggcaac cgtcattgcc acgatgcccg aaatctcgct taacgactgc attaccggaa
      481 gccctcctaa ttcatctttc atgtactcga atactaccga agtaattttt ttctgcatca
      541 gggtttttac catactctcg cattgcttat ctatctgcaa catcgagaaa atgacctggt
      601 tgccgcgcaa taacgcaatc tcttcgctgg tgaaaggggc aacttttatg acgtagtcac
      661 attcaaagac ttcatttctc gattgtgcaa ttattgctcc ggcgtttgaa tattcaaggt
      721 cagagtatcg tgttttcagc ccagccccgg tttcaattat tacatcgttg cccgagttga
      781 taatcatttc ggcagcttgg ggagtaagcg ggatgcatac ctcttcgttg tcggtattag
      841 caggtattcc aatagttatt ttctgccctt tttttctgat ttcgagtttt tcctcggcgg
      901 gcatcaattg ctgttcttca aaatgataca atccagaatg cggttgttcg gccatgatgc
      961 gaatatttga gctgtcaaat taatctaata tatcgaatat tccaattttt acccgttaat
     1021 gtcgacatta cgcgacccat tgggctgaac tgttatgtag atatttacac aatgctcggg
     1081 caaaatttct tcaattttct cgggccattc aataaagcaa agctttcctg aataaaaata
     1141 ttcttcggct ccaatatcgt atgcttcgcg aatgtttttt aaccgataac aatcgaaatg
     1201 aaaaattgtt aagcctttat tcgaagtata ttcgtttaca atggcaaaag tagggcttgt
     1261 aatgttttct tctacaccca gttcctcaca aatggctttg ataaatgtgg ttttccctgc
     1321 acccatttcg ccattaaacg caaagaccgt tgcgtttccc atttgcaaga taaactctcg
     1381 ggcagcttcg tgaatcttgt caagtgaatc tatttttatc gttttgttca tcttttacag
     1441 gcttgataat taatttatta atatagatga aatagatttt tatattttct tttaccaaaa
     1501 gtaagaaact gtttaaattt atgaaaataa tgaattaata tattgactat cagttg
//
LOCUS       DAOPUW010000538         1553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_90895_length_1553_cov_2.586782, whole genome shotgun sequence.
ACCESSION   DAOPUW010000538 DAOPUW010000000
VERSION     DAOPUW010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90895_length_1553_cov_2.586782"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PKJ43_08475"
     CDS             complement(<1..336)
                     /locus_tag="PKJ43_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72643.1"
                     /translation="MRQLYSQDIKTAEQEIRLHSKTSSPIYYFNSKLAKLFFLISLIF
                     SCSTISYAQLSEGGKPASLDMTFRKSGQLVSSVTLQPINPEQLKAEDFSFGTPFRYGV
                     VQDVAIDIKQ"
     gene            complement(553..>1553)
                     /locus_tag="PKJ43_08480"
     CDS             complement(553..>1553)
                     /locus_tag="PKJ43_08480"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNZ72644.1"
                     /translation="LNNSIANLDFVLKKSLEDLQKAKDDVTLNIASGYLEILFAKELI
                     KVAEAQIDQTQKQIERTKTLVEAGKVAEGTLLELKAQLASEELDMVTRQNALQIALMN
                     LSQMLELDSYSNFDIETPELPEMKAQASISEAAQVYGKAVLNRPEIKSADYQLRSAEA
                     QLKVAQSSLYPTLSASAGFSDPYYNVLNVAEESFGKQLRNNNSKYVGLSLNIPIFNKF
                     QSKTNIENSKLQIKNYQLQLESTKKELRKQIEQVYTNALASQKRFNANRVASKSMSES
                     FRYIEEKYNVGRVSSVEYNDAKTKLAIAESNLIQAKYEFIFRSKILDFYNGIPISL"
BASE COUNT          400 a          280 c          323 g          550 t
ORIGIN      
        1 ttgttttata tcgatggcaa catcttgaac taccccgtaa cggaaaggag tgccaaagct
       61 aaaatcttca gccttaagtt gttctgggtt gattggttga agggtaaccg atgaaacaag
      121 ttgccccgat ttgcgaaaag tcatatcgag tgaggcgggt ttgcctcctt ctgataattg
      181 cgcataacta attgttgaac agctaaatat aagggaaata aggaaaaaca gttttgctaa
      241 tttactattg aaataataaa tcggcgatga ggtttttgag tgtaatctta tttcttgttc
      301 agctgttttt atgtcttgtg aatataattg tctcattttg tgtgattaag cataatggat
      361 attccctgaa aaattagcag taaagataat tttcccggga tgttattttc gtattttatg
      421 tttttttagt gtttctttca ttgcatctgg tataacagca aaacaggaaa caagttttgt
      481 acaaaaaatg agaaaaagcg cttcttattg aaacgctttt tcaaacaata actaaatcct
      541 ataatatgtc catcacaaag atatgggtat tccgttgtag aaatctaaaa tttttgagcg
      601 gaaaatgaat tcgtatttgg cttgtatcaa gttcgattct gcaatagcaa gttttgtttt
      661 agcatcgttg tattcaacag aactaaccct gcctacattg tatttttctt cgatgtaacg
      721 gaacgattca ctcatcgatt ttgatgccac acggtttgca ttaaaccttt tctgcgatgc
      781 tagtgcattg gtataaacct gttcaatttg tttccgtagc tcttttttcg tgctttccag
      841 ttgcagttgg taatttttga tctggagctt tgagttttcg atatttgttt tgctttggaa
      901 tttgttgaaa atgggaatat tcaacgacag tccgacatac ttgctattgt tatttcggag
      961 ttgttttcca aagctttctt cagcaacatt taaaacatta taataggggt cgctgaagcc
     1021 ggcacttgca cttaatgttg ggtagaggct actttgggca acctttagct gagcctcggc
     1081 acttcggagc tggtagtcgg cactcttgat ttcgggacgg tttagcaccg ctttcccata
     1141 aacctgtgcg gcttctgaaa tcgaagcctg cgctttcatt tctggtaatt cgggcgtttc
     1201 aatatcgaaa ttggaatatg aatccagttc aagcatctgc gaaaggttca tcagcgctat
     1261 ttgcaatgcg ttttgacggg ttaccatgtc gagttcttcg ctggcaagct gagctttaag
     1321 ttcgagaagt gtcccttctg ctacttttcc tgcttcaaca agggttttcg ttctttcgat
     1381 ttgcttttgg gtttgatcaa tttgtgcttc ggccactttt atcagttctt tggcaaaaag
     1441 tatctcaagg tatcccgagg caatattcag cgtaacgtca tccttcgctt tctgaaggtc
     1501 ttcgaggctt ttcttcagaa caaagtcgag gttggcaatg ctattgttca atc
//
LOCUS       DAOPUW010000539         1550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_91146_length_1550_cov_3.466890, whole genome shotgun sequence.
ACCESSION   DAOPUW010000539 DAOPUW010000000
VERSION     DAOPUW010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91146_length_1550_cov_3.466890"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /gene="rfbB"
                     /locus_tag="PKJ43_08485"
     CDS             complement(<1..952)
                     /gene="rfbB"
                     /locus_tag="PKJ43_08485"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNZ72645.1"
                     /translation="MKKIIITGGAGFIGSHVVRRFVRKYTDSQIFNLDALTYAGNLEN
                     LSDIKDCSNYTFVKGDITDGDFITRLFEEHQFDGVIHLAAESHVDRSITNPMSFIMTN
                     IVGTVNLLNAANHIWKGNYEGKRFYHISTDEVYGSLGEEGFFTEETHYDPQSPYSASK
                     ASSDHLVRAYHNTYGLPVVLSNCSNNYGPNQFPEKLIPLIINNIENNKPLPVYGQGLN
                     VRDWLYVEDHASAIDLIFNEGEIGETYNIGGNNEWRNIDIVKKICEIMDRKLGRENGT
                     SEKLITYVKDRAGHDLRYAIDATKIKKCLGWEPSLQFEEGI"
     gene            complement(1150..>1550)
                     /locus_tag="PKJ43_08490"
     CDS             complement(1150..>1550)
                     /locus_tag="PKJ43_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72646.1"
                     /translation="FVIPSPYFDVAVSASNELWAANTGMHSLQHYDENGNLLDSWEKI
                     SNNIDGFCGCCNPAHFALMTDGRFVTSEKRLTRIKIYSQKGELESVVASPDVFVEEGF
                     APEVAVIGDTVVALDFDKKMIRIFGKSESK"
BASE COUNT          412 a          322 c          311 g          505 t
ORIGIN      
        1 caattccttc ttcaaattga agtgagggct cccagcctaa gcattttttt atttttgttg
       61 catcaatagc ataccgcaaa tcgtggcctg cacggtcttt cacatacgtt atcagttttt
      121 ctgatgtccc gttttcgcgg cccagtttgc gatccattat ctcacatatt tttttcacta
      181 tgtcgatgtt acgccactcg ttgttgcccc cgatattgta tgtttcgccg atttcccctt
      241 cattgaaaat cagatcgatg gctgaggcgt ggtcttcaac gtaaagccaa tcgcgtacgt
      301 ttaagccctg accgtaaacc ggaaggggtt tattgttttc tatgttgtta attattaatg
      361 gaatgagttt ttcggggaat tgatttggcc cgtagttgtt tgaacagttc gacagcacta
      421 ccggcaaacc ataggtgttg tggtatgccc ttaccaagtg gtctgaactt gctttcgatg
      481 ccgaatatgg gctttgtgga tcataatggg tttcttcggt gaaaaatcct tcttctccca
      541 gtgagccata cacttcgtcg gtcgagatat ggtaaaaacg tttgccttcg taatttcctt
      601 tccagatgtg atttgcggca ttcaataagt taactgtccc aacgatatta gtcataatga
      661 atgacatagg gtttgtaatc gatcggtcga catgactttc tgcggccaaa tggatcacac
      721 catcgaattg gtgttcttca aaaagccggg ttatgaagtc tccgtcggtg atgtcgcctt
      781 ttacaaaagt ataattgctg caatctttta tgtcggataa gttttcgaga ttgccagcgt
      841 aggttaatgc atcgaggtta aatatctgac tatcggtata ttttctaaca aatctgcgaa
      901 ctacatgaga accaatgaat ccggcgcctc cggttatgat aatttttttc attttaatta
      961 cgtataaatt ttacttgctg ataagcttaa acggatgcaa tttgaatgtt gtatctttta
     1021 gtacaaaaat aggtatacca atctattttg caataaacat ttactatgga aaaaactacc
     1081 cgcggagcat tttattatgg atactgaagg gacttgatgc aaaaatgaat tcgaatattg
     1141 acttttcagc tatttggact cacttttccc aaagatcctg atcatttttt tatcgaaatc
     1201 gagggcaact acagtatcgc caatcacggc gacttcaggg gcaaatcctt cttctacaaa
     1261 aacatcagga cttgcaacaa ccgattcgag ttctcctttt tgactgtaga ttttaattct
     1321 cgtaagcctt ttttcactgg tgacaaaccg tccatcggtc attaacgcaa aatgtgccgg
     1381 attacaacat ccgcagaaac catcaatgtt gttcgatatt ttctcccagg aatcgagcag
     1441 atttccattt tcatcgtagt gttggagcga atgcatgcca gtattggcgg cccaaagttc
     1501 attcgatgcg ctaacggcaa catcaaaata aggacttggg ataacgaaac
//
LOCUS       DAOPUW010000540         1549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_91231_length_1549_cov_3.093708, whole genome shotgun sequence.
ACCESSION   DAOPUW010000540 DAOPUW010000000
VERSION     DAOPUW010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91231_length_1549_cov_3.093708"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PKJ43_08495"
     CDS             complement(<1..235)
                     /locus_tag="PKJ43_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HNZ72647.1"
                     /translation="MSIEEIQQEIIAEFSVFDDWMDKYSYLIELGNGIEALDEKYKVD
                     QNLIRGCQSQVWLNAECRDEIVYYQGESDALIVK"
     gene            525..>1549
                     /locus_tag="PKJ43_08500"
     CDS             525..>1549
                     /locus_tag="PKJ43_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNZ72648.1"
                     /translation="MKAISLLLIITLLCGSYQAMAQKKTPGKKVEVTESEEDSEESYE
                     EENSDSEEAEEEAEEVEEEASTPKEQTLSLFSKYDFVAGDKVVFFEDFSQDAVGDFPA
                     LWNTNASGEVCITGEFPGKWLKMNADGMFFLEKGFNFPKNFTLEFDVLPMQEESGDIG
                     RIILALFSPSEGDLYNSGGVPGDAGIEIAMGSYFSYRAYDENKDLQNDVWNKKMPKNE
                     VSHVSIWVQNSRLRLYALGSKLLDIPQALTKGFDYKQIRFCTDEIEATKFLISNIRVA
                     EAGTDQRSKFLTDGKLISYGILFDVNSDKVKPESYGALKEMADILKENPSVRVKVVGH
                     TDSDGNDA"
BASE COUNT          541 a          315 c          295 g          398 t
ORIGIN      
        1 ctttaacaat caatgcatca ctttctccct gataatacac tatttcatcg cggcactcag
       61 cgttaagcca aacttgcgac tggcaacccc gaatcaggtt ttggtcaact ttatatttct
      121 catcaagagc ctcaatgcca tttcccagtt caatcaggta agaatattta tccatccaat
      181 catcgaaaac cgaaaactct gcaataatct cttgctgtat ttcttcaata ctcattatac
      241 tatatttaat tttgaagata caaaggtcgt tttttttcaa aaaaaacgca gaaaaaatat
      301 cattccataa aaataaatta agccaaaaga aatgcaagca ggaaaaaagc tctacatcca
      361 gaaaacaaac atctcatgag cattatgccg aatcgaaata caaagttaac tccgctttta
      421 ataaaaatat atatcttcgt ttacagaaaa caggtcaaca atgaatgtga cctttacgcc
      481 aactaataat ataaaccaga acaattctct aaaattcaaa tcatatgaaa gccatctcat
      541 tattattgat cattaccctc ttgtgcggca gttaccaagc catggcacaa aagaaaacac
      601 caggaaaaaa agttgaggta acagaatctg aagaagatag tgaagaaagc tacgaagaag
      661 aaaactccga ttctgaagaa gcagaagagg aagcagaaga agtggaagaa gaagcctcta
      721 cccccaagga acaaacacta agcctgttct caaaatacga ttttgtagct ggtgataaag
      781 tggttttttt cgaagacttc tcacaagatg cagtgggcga ttttccggca ctttggaaca
      841 caaatgcttc gggcgaagtt tgtataacag gcgaatttcc aggcaaatgg cttaagatga
      901 acgccgacgg aatgtttttt cttgaaaaag gtttcaattt tccaaaaaac ttcacactcg
      961 agttcgatgt gttgcccatg caagaggaaa gtggcgatat tggccgtata atccttgctt
     1021 tattttcgcc atccgaaggc gacttataca attcgggagg agtacctggc gatgcaggga
     1081 tcgaaattgc tatgggaagt tatttttcct atcgcgctta cgatgaaaac aaagatctgc
     1141 aaaacgatgt atggaataaa aaaatgccaa aaaatgaagt aagccatgtt tcgatatggg
     1201 ttcaaaattc gaggctcagg ttatatgccc ttggctccaa attactcgac atcccgcaag
     1261 cactaacaaa agggttcgac tacaagcaga ttcgtttctg caccgacgaa attgaagcta
     1321 ccaaatttct tataagtaat atacgagttg cagaagcagg aaccgatcag cgcagcaagt
     1381 tccttaccga tgggaaactg attagttatg gtatcctttt cgacgtgaat tccgacaaag
     1441 tgaaacccga atcgtacgga gcccttaaag aaatggctga tatacttaaa gaaaacccgt
     1501 cggtacgggt gaaagtggtt ggccataccg attcagacgg caacgatgc
//
LOCUS       DAOPUW010000541         1549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_91233_length_1549_cov_3.036814, whole genome shotgun sequence.
ACCESSION   DAOPUW010000541 DAOPUW010000000
VERSION     DAOPUW010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91233_length_1549_cov_3.036814"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1549
                     /locus_tag="PKJ43_08505"
     CDS             <1..>1549
                     /locus_tag="PKJ43_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNZ72649.1"
                     /translation="RGKDMILGPSINIQRIPTGGRTYEYLSEDPFLSSRLSVEYTKGV
                     QDNGVAACLKHYALNNQENNRGTVNVVIGERAMREIYLPPFRAAVEEGDAYGIMSAYN
                     KVNGWWCGENDILLNKILRNDWGFGGLVVSDWGGTHSTVEAAKYGLNVEMPGKKYLGQ
                     ALLDSVNSGAISEDIINQRVREILRVRKAITPVPAEEANKQMTSQPEQQKIAYNVACK
                     SIVLLKNEGILPLNIENKPLIAIIGANATQTMASGGLGAGVKALYEITPLEGLKNKIG
                     DRAEIIFAQGYQPEAYSYGRFRKTPKDIEAENIEKEKIAQQLTKEAIELAAKADIVIF
                     IGGNNRAVETEGRDRENIFLPSGQDELIKKIAQANSNVITVLASGAPNDLNTVKPLSK
                     ALLISWFNGSEGGNALADVLLGTISPSGRLPFTIPLRLEDSPAYAMGNYPQGAKNEDI
                     FANLVEKKDDEKKTETKETQKPENDPNTAIYSEESLVGYRWFDTKNIQVMYPFGYGLT
                     YSTFEYT"
BASE COUNT          521 a          313 c          354 g          361 t
ORIGIN      
        1 tgagaggaaa agacatgatc cttggccctt caatcaacat ccagcgaatc ccaaccggcg
       61 gaagaaccta tgaatacctc agtgaagatc cattcctgag tagccgcctt tcggttgaat
      121 ataccaaagg ggtacaagac aacggagtag cagcatgtct taagcactat gccctgaata
      181 accaggaaaa caaccggggg acagtaaatg ttgtgattgg agaaagggct atgcgggaaa
      241 tatatttgcc ccctttcagg gctgctgttg aagaaggtga tgcttacgga atcatgtcgg
      301 cctacaataa agtaaatggt tggtggtgtg gggaaaatga tatactacta aataaaattt
      361 tgcgcaatga ttggggattt ggcggtcttg tagtttcaga ctggggcgga acccacagca
      421 cggtagaggc agcaaaatac ggacttaacg tagaaatgcc aggcaaaaaa taccttggtc
      481 aagcgctact cgattctgtt aactcaggtg ctatttcaga agatattata aaccaaagag
      541 ttcgcgaaat acttagggta cgtaaagcga tcactccggt accagcagaa gaagcaaata
      601 agcaaatgac ttcgcagccc gaacagcaaa aaattgccta taacgtagcc tgcaaatcaa
      661 ttgttttgtt gaaaaacgaa ggtatattac cgctaaacat agaaaacaag ccccttatag
      721 ccattattgg tgccaatgcc acacaaacca tggcgtcagg aggattgggt gcaggagtga
      781 aagctcttta cgaaataacc ccgcttgaag gcttgaaaaa caagatagga gaccgtgccg
      841 aaattatttt cgcacaagga tatcaacccg aagcatactc ctatggccgc ttcagaaaaa
      901 cgcccaaaga tattgaagcc gaaaacatag aaaaagagaa aatcgcacag cagcttacca
      961 aagaagccat agaattggca gcaaaagctg acattgtaat atttatcggt ggaaataacc
     1021 gcgccgttga gactgaagga cgtgaccgtg aaaatatctt tttgccctcg ggacaagatg
     1081 aactcattaa aaaaattgct caggctaact caaatgtaat tacagtattg gcgagcgggg
     1141 cacctaacga tttgaacacg gtaaagccat tatcgaaagc cttgttaata tcgtggttta
     1201 acggctctga aggtggaaat gccttagccg atgtattact tggtacaatt tcgccaagcg
     1261 gccgtttgcc ttttacaata ccgcttcgtc ttgaagattc tccagcctat gcaatgggaa
     1321 attatcctca aggcgctaag aatgaggata tttttgccaa tttggtcgag aaaaaagacg
     1381 acgaaaagaa aactgaaaca aaagaaacac aaaagcctga aaatgatccc aatacagcaa
     1441 tatattcaga agagtcgtta gtgggttacc gttggttcga tacgaaaaat attcaggtga
     1501 tgtacccctt tggatacggg cttacctatt ccacttttga gtatactaa
//
LOCUS       DAOPUW010000542         1545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_91559_length_1545_cov_3.040940, whole genome shotgun sequence.
ACCESSION   DAOPUW010000542 DAOPUW010000000
VERSION     DAOPUW010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91559_length_1545_cov_3.040940"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="PKJ43_08510"
     CDS             <1..947
                     /locus_tag="PKJ43_08510"
                     /inference="COORDINATES: protein motif:HMM:NF039585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HNZ72650.1"
                     /translation="RRRFWKGEMSDDKQAAYETLFECLNVVSQLMSPVAPFFSEWMHR
                     NLNEGSASVHLSYLVKADKSLLDKDLEERMELAQRSCSLILSLRKKEKIKVRQPLSKI
                     LIPVVNAKTQAQFEKVESYILSEVNVKAIEYISDASGVVKKKIKPNFKELGKRVGSKM
                     KAVQNAVLAFTADDISQIERDQKYVLNLEGESFELFLADVDIQTEDIPGWLVASEGAL
                     TVALDVTLTDALRQEGNAREFVNKVQNLRKDKNFKVLDRIKVKVIANDMLKEAIVNFH
                     DYIANEILAKEIEVVDTLDSFDELEYNEETIKVFVAVV"
     gene            972..>1545
                     /locus_tag="PKJ43_08515"
     CDS             972..>1545
                     /locus_tag="PKJ43_08515"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010424226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HNZ72651.1"
                     /translation="MKRKYIISALIIFTILALDQGLKIWVKTHMIENQNIPQHYDSWW
                     NIHFVENPGMAFSIELPGPYGKLVLSVFRFFAIGFIFYMIVDLIRKNKPEGLVYCAAA
                     IFAGAAGNLIDSAVYGLIFTDSYGRVAEMFPAKGYAGFMQGYVVDMLWFPILRGEFPK
                     WLPLWGGEPFEFFRPVFNIADAAITVSVFFV"
BASE COUNT          455 a          254 c          382 g          454 t
ORIGIN      
        1 accgcagacg tttctggaag ggcgaaatga gcgatgacaa acaagccgct tacgaaacat
       61 tgttcgagtg tttgaatgta gtgtcgcagt taatgagtcc tgttgctcca ttttttagcg
      121 aatggatgca ccgcaatttg aacgaaggca gcgcttcggt acatctttcc tatttagtga
      181 aagccgataa aagcttgctg gataaggatt tggaagagcg catggaactg gcacaacgct
      241 cgtgttcgtt gattctttca ttgcgtaaga aagagaaaat aaaagttcgt cagccgttgt
      301 ctaaaatttt gattccggtg gtgaatgcca aaacacaagc acaattcgaa aaggtcgaaa
      361 gctacatttt gagtgaggtg aatgtgaagg ctattgaata tatcagcgat gcatcgggtg
      421 tggtaaaaaa gaaaatcaaa ccgaatttca aagaattagg aaaacgtgtt ggctcgaaaa
      481 tgaaagccgt gcaaaacgcg gtattggctt ttactgccga tgatatctcg caaattgaac
      541 gcgatcaaaa gtatgtgttg aatttagaag gagaatcatt cgaattgttt ttggccgatg
      601 tggatattca aactgaagat attccgggtt ggttagtagc ttcagaaggt gcgttgacag
      661 tagctttgga tgtaacttta acagatgcat tacgtcagga aggcaatgcg cgtgagttcg
      721 tgaataaggt gcagaatctc cgaaaagaca aaaatttcaa agtgcttgac cgcattaaag
      781 tgaaagtgat tgcgaacgac atgctgaaag aggccatcgt taattttcat gattatatcg
      841 ccaacgaaat tttagcgaaa gaaattgaag tggtagacac tttggatagc tttgacgagt
      901 tagagtataa tgaagaaacc attaaagtat ttgttgccgt tgtgtaacag tattcataaa
      961 atcatcaagc aatgaaaaga aagtacatca tatctgccct tattattttt accattttgg
     1021 ccctcgacca aggtttgaaa atttgggtaa aaacccacat gatagagaat cagaacatac
     1081 ctcagcacta cgacagttgg tggaatattc attttgtaga aaacccggga atggctttca
     1141 gtattgagtt gcccggtcct tatggaaaat tggttttgtc agtttttagg tttttcgcca
     1201 ttgggtttat cttctatatg atagtagatt taattcgcaa gaataagccg gaaggtttgg
     1261 tgtattgtgc agccgccatt tttgcaggag cagccggaaa ccttatcgat agtgctgttt
     1321 acggattaat ttttaccgat agttatggtc gtgtggcaga gatgttcccg gcaaaaggct
     1381 atgcgggctt catgcagggg tatgtagtag acatgttgtg gttccctatt ttgagaggcg
     1441 aattcccgaa atggttgccg ttatggggcg gtgagccgtt tgagtttttt cgtccggtgt
     1501 tcaacattgc cgatgctgca ataacagtaa gcgttttctt tgtat
//
LOCUS       DAOPUW010000543         1542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_91803_length_1542_cov_3.418292, whole genome shotgun sequence.
ACCESSION   DAOPUW010000543 DAOPUW010000000
VERSION     DAOPUW010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91803_length_1542_cov_3.418292"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..1441)
                     /gene="trpB"
                     /locus_tag="PKJ43_08520"
     CDS             complement(242..1441)
                     /gene="trpB"
                     /locus_tag="PKJ43_08520"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNZ72652.1"
                     /translation="MSNYFKTVPTKEGFFGEYGGSFIPPQLQEEMDKITDAYFSISKS
                     HEFISELRSIRKHFQGRPTPVYFCKRLSEKAGSRIYLKREDLNHSGAHKLNHCMGEAL
                     LAKYLGKKKLIAETGAGQHGVALATAAAYFGLECEIHMGEVDIKKEHPNVVRMKILGA
                     KVVPVSHGLKTLKEAVDSAFEAYLVDPINSIYCIGSVVGPHPFPMMVRDFQRVVGIEA
                     REQFYEMTGELPDNVVACVGGGSNAAGIFSAFLEDDEVSLWGVEPAGRSYKTGDHACS
                     MKFGTTGIIHGFRCKVLQDEKGEPSPVYSVASGLDYPGVGPEHCMLNEKKRVNYVDAT
                     DKETINAFYELSRLEGIIPALESSHAVAYAIKMSKEKKRESILVNLSGRGDKDIDFIV
                     DNYGLPE"
BASE COUNT          432 a          324 c          316 g          470 t
ORIGIN      
        1 agggttagat catgaaacgg cacttttacc ttcgtttgct aaagcagttg tttcgtttaa
       61 atcgtattaa aaatatttga gaatttgtag acaacacacc aagagataga taagaggggg
      121 attcatattc ctctcttttt ttgttattga gagaacgggt attaaaggag gaaatgaaaa
      181 tacaaaaaaa gccctccata aaggaaggct ttctcttttc tatttataat ataagaatgg
      241 tttattcggg caaaccgtag ttgtcgacaa taaaatcgat gtctttgtct ccacggccgc
      301 taaggttaac caatattgat tcacgtttct tttctttcga catttttatt gcataagcaa
      361 cagcgtgcga gctttctaga gcggggataa tcccttcaag gcggctcagt tcgtaaaatg
      421 cattgatggt ttctttgtcg gtagcatcta cataattaac acgcttcttt tcgttcaaca
      481 tacaatgttc tggcccaaca ccaggataat cgagtccgct tgctacagag taaacaggag
      541 aaggttcgcc tttttcatct tgtagtactt tacatctgaa cccgtggata attccagtgg
      601 ttccgaattt cattgagcac gcatgatcac cagtcttgta tgaacggcca gctggctcga
      661 caccccaaag tgaaacttca tcatcttcaa ggaaagcaga gaaaatccca gcagcattac
      721 tgcctccacc tacgcaagcc accacgttgt ctggaagttc accggtcatc tcgtaaaact
      781 gttcgcgggc ttcaatgcct acaacgcgct gaaaatcacg caccatcatt gggaatgggt
      841 gaggtcctac taccgatccg atgcaataaa ttgaatttat agggtctact aagtaggctt
      901 cgaaagctga gtcaacagct tctttcagcg tttttagccc gtgacttaca ggaacaactt
      961 tcgctccaag aattttcatg cgaaccacat tggggtgctc tttctttatg tcaacttcac
     1021 ccatgtgtat ttcacactcc aggccaaaat aggctgctgc agttgctaat gctacaccat
     1081 gttgccctgc gcctgtttcg gcaataagtt ttttcttacc taaatatttg gccaacaggg
     1141 cttcacccat gcaatggttg agtttgtgtg cgccagagtg gttgaggtct tcccgtttaa
     1201 ggtaaatgcg gcttcctgct ttttcagata atcgtttgca gaaatatacc ggggtgggcc
     1261 gcccttgaaa atgtttacgg atgcttctta attcagaaat aaattcgtgt gatttgctta
     1321 tcgagaaata ggcatcggtg attttatcca tttcttcttg aagctggggg ggaatgaaac
     1381 ttcctccata ctcaccaaag aaaccttctt tagtgggaac tgttttaaaa taattgctca
     1441 tactttaaat tttgcaaagg gttattccgg catttccgga gtggttataa aagtatcgaa
     1501 aaaatagaaa aagtgaagta tttcatgata aaaatcattc ac
//
LOCUS       DAOPUW010000544         1534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_92608_length_1534_cov_2.272481, whole genome shotgun sequence.
ACCESSION   DAOPUW010000544 DAOPUW010000000
VERSION     DAOPUW010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92608_length_1534_cov_2.272481"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PKJ43_08525"
     CDS             complement(<1..433)
                     /locus_tag="PKJ43_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ72653.1"
                     /translation="MKCIVIDDDDISRRVIEDFITRTSFLTLEHSFAGAIEAVNFLKS
                     GNKVNLIFLDIEMPEMTGIDFLNSLDQPPQVIIVSSKEKYALKAFEYHVTDYLLKPVN
                     YARFFKAANKALESFDRSFIAPGEDKEIFVKKSNSLVKVKFD"
     gene            complement(491..1222)
                     /locus_tag="PKJ43_08530"
     CDS             complement(491..1222)
                     /locus_tag="PKJ43_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNZ72654.1"
                     /translation="MNRKPSDIFELSPQIVQGLIGALSECVLEKRVELFQSILEERTR
                     YVTVVLEDIHQSQNASAAIRSCECFGIQDIHIIENNHKFSVNPKVSMGAAKWLNIYKY
                     NHKQNNSLEAVEHLKREGYRIVGTSPHASDVSLDNFSIEKGKFALFFGNELNGLSEIV
                     LKNSSEIVKIPTVGFTESLNLSVSVAICIHKLTARLRNSAIDYRLTEREKAILLLQWL
                     RLTVKSWRSIEKRFLAEQLFHNINK"
     gene            complement(1325..>1534)
                     /locus_tag="PKJ43_08535"
     CDS             complement(1325..>1534)
                     /locus_tag="PKJ43_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNZ72655.1"
                     /translation="KKALDAFVKTTGNALKKGGRVSLVGFGSFTVIKRSARTGRNPQT
                     GKPINIPAKKVVKFKPGSDLNSKVN"
BASE COUNT          419 a          314 c          311 g          490 t
ORIGIN      
        1 catcgaattt aacctttacc agggaattac tttttttaac aaaaatctct ttgtcttctc
       61 ctggagcaat aaaactcctg tcgaagcttt ccaatgcttt attggctgct ttaaaaaaac
      121 gggcgtagtt tacaggcttc agcagataat cggtaacatg gtattcaaat gccttcaggg
      181 catatttctc cttggaggag acaataatga cctgaggagg ttgatctaag gagttaagaa
      241 agtcaatccc tgtcatttcg ggcatttcga tatcgaggaa tatgagatta accttatttc
      301 cgcttttcag aaagttaacg gcttcgatgg ctccggcaaa ggagtgttca agggttaaga
      361 aacttgtacg ggtgataaaa tcttcaatta ctctcctcga aatatcatcg tcatctataa
      421 ctatgcattt catggcagta gaatttttat ttctaacgaa agtatggaaa aaattccaat
      481 aatcctccct ttatttgtta atattgtgaa ataattgttc agccaagaat cttttctcaa
      541 tcgacctcca actttttacc gttaatctta gccactgcaa aagcagtatg gctttttctc
      601 tctcggttag tcgataatca atagctgagt tacgtagcct ggcggtaagt ttatgaatgc
      661 aaattgccac agaaacggag aggtttaaac tttcggtaaa tccaaccgta ggaatcttaa
      721 cgatctcgct ggaatttttc aggactattt ccgacaatcc gttcagttca ttcccaaaga
      781 ataaggcaaa cttacccttt tcgatggaaa agttgtcaag gctgacgtcg gaagcgtgtg
      841 gcgaagtccc tacgattctg tatccttccc ttttcagatg ctccactgct tccagtgagt
      901 tattctgctt gtgattgtat ttgtaaatgt tgagccattt ggccgccccc atcgatactt
      961 ttgggtttac ggagaattta tggttattct ctataatatg aatatcctga atgccaaaac
     1021 attcgcatga acgaatggct gcgctggcgt tttgcgattg gtgaatatcc tccaggacca
     1081 cggtaacata gcgtgttcgt tcctctaaga tggattgaaa tagctccacc cgtttttcca
     1141 gcacgcattc cgatagggcc ccgatgagcc cttgaacaat ctgtggcgac agttcgaaaa
     1201 tgtcagatgg cttcctattc atgtatgcta tgcaatgagt agttcgaaac aactgtatgt
     1261 attggcaaaa aaataagtgg ggcaccagtt gcgatgcccc acttattata tttcttgaaa
     1321 gatcttagtt tactttcgaa ttaagatcgc tacccggttt gaattttaca acttttttag
     1381 cggggatgtt aattggtttg ccggtttgag gattacggcc ggtccttgcg cttcttttaa
     1441 taacggtgaa agaaccaaaa ccaacaaggg atactctacc gccttttttt agggcgttac
     1501 cggtagtttt tacaaaagca tccaatgctt tctt
//
LOCUS       DAOPUW010000545         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_92739_length_1532_cov_3.415030, whole genome shotgun sequence.
ACCESSION   DAOPUW010000545 DAOPUW010000000
VERSION     DAOPUW010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92739_length_1532_cov_3.415030"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1268)
                     /gene="lysA"
                     /locus_tag="PKJ43_08540"
     CDS             complement(3..1268)
                     /gene="lysA"
                     /locus_tag="PKJ43_08540"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNZ72656.1"
                     /translation="MQGKTLPFSKEQIERIIEKYPTPFHIYDEKGMVEYARYFNQCFD
                     WNEGFKEFYAIKAAPNPYLMKILRNEGFGIDCSSMAELLLAEKVGMRGEEIMFTSNDT
                     PAEEFKKAIELGAIINLDDISHIDFLNETAGLPDLVSFRYNPGALKGGNIIIGNPEDS
                     KYGFTREQLFEGYRKLKSMGVKRFGLHTMVASNELDPDYFIETAEIIFDLIVDLHKEL
                     GIRFEFANLGGGIGIPYRNEHRPVNMKKVSKGIKERYESMIVANGLAPLRIYFESGRA
                     ITGPFGFLVSKVLHLKETYKKYVGLDASMANLMRPALYGAYHHITVLGKEDQPHTNLY
                     DVTGSLCENNDKFAINRLLPEIKRGDIVVIHDAGAHGHSMGFNYNGKTRSAELLLREN
                     GEVVQIRRRETIDDYFATLDFDGLTKFDV"
BASE COUNT          400 a          330 c          323 g          479 t
ORIGIN      
        1 atttaaacgt cgaattttgt cagtccatcg aaatccagtg tggcaaaata atcgtcgatt
       61 gtttccctgc ggcgaatttg cactacttca ccattttcgc gcagcaacag ctcggccgaa
      121 cgggttttcc cgttatagtt gaaccccatg gagtgcccat gagccccggc atcgtggatt
      181 actactatat cgccacgttt tatctcgggt aacaagcggt taatagcaaa tttatcgttg
      241 ttttcgcaaa gcgaacctgt tacatcatat aagttggtgt gaggctggtc ttctttccca
      301 agtacggtaa tatgatggta tgccccgtat agtgccggac gcattaaatt ggccatgctt
      361 gcatcgagtc caacatattt cttgtaggtt tctttcagat gaagcacttt cgaaaccagg
      421 aaaccgaaag gcccggtaat tgccctgccc gattcgaaat atatcctgag gggtgctaat
      481 ccgttggcta caatcatcga ttcgtagcgt tcttttattc ctttacttac ctttttcatg
      541 ttgacaggcc ggtgttcatt cctgtaggga attcctatgc caccgcctag gttggcaaat
      601 tcgaagcgga tacccagttc tttatgcagg tcaacaataa ggtcgaagat aatctcggct
      661 gtttcgataa agtaatcagg atcgagttca tttgaagcaa ccatggtgtg caggccaaac
      721 cgtttcactc ccatcgactt cagcttccgg tatccttcga agagctgttc gcgggtgaag
      781 ccatacttcg agtcttcagg atttccaatg ataatgtttc caccctttaa tgctccgggg
      841 ttgtacctga agctgaccag gtcgggcagg ccggcagtct cgttaaggaa atcgatatgg
      901 ctgatatcgt cgaggttgat aatcgcgcca agttcaatgg cttttttgaa ttcttctgcg
      961 ggcgtatcat ttgaggtaaa catgatttct tcacctctca taccaacttt ttcggccaaa
     1021 agtaactcgg ccatagaact gcagtcgatg ccaaagcctt cattgcgaag aattttcatt
     1081 aagtaggggt ttggagctgc ttttatggca taaaactctt taaaaccttc gttccaatcg
     1141 aagcattgat taaaataacg ggcatattca accatccctt tttcatcgta aatatgaaac
     1201 ggggtaggat atttttcaat aattctttca atctgttctt tcgaaaatgg caatgttttc
     1261 ccttgcataa tgatattctt ttcgattttc tggcaaagat atcattttgg caattttttg
     1321 ccggtcgaaa catctaagaa accgagaagt acgtaagaat taaggttgac gaaatactgc
     1381 acattcttat tctgaatatt tttaaatcgc aatctctgca ggcgttgaat ttacaattgc
     1441 aaggttttac tcatttcgta aagatttaat catttttgtc tggtcaattt gcctgaagga
     1501 aatggcgata atccagaaca gggcattaag aa
//
LOCUS       DAOPUW010000546         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_92774_length_1532_cov_2.448883, whole genome shotgun sequence.
ACCESSION   DAOPUW010000546 DAOPUW010000000
VERSION     DAOPUW010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92774_length_1532_cov_2.448883"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PKJ43_08545"
     CDS             complement(<1..260)
                     /locus_tag="PKJ43_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HNZ72657.1"
                     /translation="MDCTPIFKKVLKASRSLNLVSVETINSVLNHVADAAIANSDAIL
                     VENAKDLARMDKSDPKYDRLKLTIDRIAGIASDWGNVASLPSP"
     gene            complement(295..1380)
                     /gene="proB"
                     /locus_tag="PKJ43_08550"
     CDS             complement(295..1380)
                     /gene="proB"
                     /locus_tag="PKJ43_08550"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNZ72658.1"
                     /translation="MHTRKFKRIAVKIGSNVLAGAGGLDFVRIAHLVDQISSLKKEGI
                     EVILISSGAVASGRSAFPVESRIDTVSARQLWSAIGQVKMMNTYSEYFDKHGITCAQV
                     LTTKENFSDRKHYLNMKNCISTMLEYGVLPIVNENDTISVTELMFTDNDELSGLISSM
                     MDCDALLILSNVNGIYNGTPGEEGVSVIPEISVADENFESFISTQKSGFGRGGMHTKF
                     SVARKIALEGIPVFIANGKVDNIITDIVHHRKVIYTLFRPNGKRLKSNKKWLAHSTSF
                     AKGIVVVNEGAKDALLSGGANSLLLIGVVEIKGYFEKGDVIRIQDEQGNSLGLGKAQY
                     DSDTAIEFIGKKAEKPLIYYDYMYLNE"
BASE COUNT          440 a          349 c          294 g          449 t
ORIGIN      
        1 ggcgaaggca aactggccac attaccccaa tcggaagcaa tcccggcaat gcggtcaata
       61 gttaatttca gccgatcata ttttgggtct gatttgtcca tgcgagccaa gtctttggca
      121 ttttcaacca aaattgcatc agaatttgca atagcagcat cggcaacatg gttcaacacg
      181 ctattaatcg tttcaaccga taccaggttc agtgaacgac tggctttcag cacttttttg
      241 aaaattggag tacaatccat cttcttaatt tttatggttc aattcaacgt agtattactc
      301 gttgagatac atataatcgt aataaatcag cggcttttcc gctttcttgc caataaattc
      361 aatagcagta tcagaatcat actgagcctt tcccaacccc aggctattgc cttgttcatc
      421 ctggatgcgt atgacatctc ccttttcgaa atacccttta atttcgacaa cgccgatcag
      481 caaaaggctg ttggctcctc cgctcaatag ggcatctttt gccccctcat taaccacaac
      541 gatacctttg gcaaaactgg tggaatgggc aagccacttt ttattgctct tcaggcgttt
      601 cccattggga cgaaaaaggg tataaataac tttccgatga tggacaatat cggtaatgat
      661 gttgtcgact ttgccgtttg caatgaaaac cggtatgccc tcgagagcga tctttctggc
      721 aacactgaat tttgtatgca ttccaccacg tccgaagcca gatttttggg tagaaatgaa
      781 actttcgaaa ttttcatcgg ctaccgaaat ttcaggaata acagaaacac cttcttcgcc
      841 cggagtacca ttgtaaatcc catttacatt agaaagaatc agcaacgcat cgcaatccat
      901 catcgacgat atcaagcccg acaattcatc gttatcggta aacatcagct cggtcacaga
      961 gattgtatcg ttctcgttta caataggaag aacaccatat tcgagcatcg tcgaaataca
     1021 gttcttcata ttgagataat gcttccggtc gctaaaattt tcttttgtgg taagtacttg
     1081 tgcgcaggta attccatgct tgtcgaaata ttcagaatag gtattcatca ttttcacctg
     1141 tccaattgct gaccaaagtt ggcgggcaga aacggtgtcg atacgacttt ccactggaaa
     1201 tgcagagcgc ccagaggcaa cagcccccga cgagataagg atcacttcaa tcccttcttt
     1261 tttaaggctt gaaatttggt cgaccagatg ggcaatcctg acaaaatcga ggccaccagc
     1321 cccggccagc acattgcttc ctatttttac ggcaatccgt ttgaattttc gagtgtgcat
     1381 tggtttagta tgcttattga aaacatgaca aatgtcatta tttttatttt aatgacaaag
     1441 aaacgttgcc taaattattg gaaaaaagct aaaaggataa aaatcaacta tgattttcga
     1501 tcgattttgt tgtacgaagc cagcaaccct tt
//
LOCUS       DAOPUW010000547         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_92775_length_1532_cov_2.386594, whole genome shotgun sequence.
ACCESSION   DAOPUW010000547 DAOPUW010000000
VERSION     DAOPUW010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92775_length_1532_cov_2.386594"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PKJ43_08555"
     CDS             complement(<1..705)
                     /locus_tag="PKJ43_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNZ72659.1"
                     /translation="MFEKSIHLEGVDLLEFYGVNNTKLDLIKSLFPKLKIIARGDMIK
                     VKGSEEDSFHFEKKINELLDYYHDFHSLNEKVIIQIVAEQSPIEGQTTENSDVLVFGI
                     NGRPVRARTPNQRKLVEATDENDLIFAIGPAGTGKTYTAIALAVKALKNREIRKIILS
                     RPAVEAGERLGFLPGDLKEKIDPYLQPLYDALGDMIPQKKLEEFMKDKVIEIAPLAFM
                     RGRTLSNAFVILDEAQN"
     gene            complement(821..>1532)
                     /locus_tag="PKJ43_08560"
     CDS             complement(821..>1532)
                     /locus_tag="PKJ43_08560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ72660.1"
                     /translation="AEYKLTIAAGDTIGIFQFAGQETTGWNSIVGGYIYIGKVSAVQA
                     PAKAAITFVVDDSALKSSTGFGLKGSWNTATGEFDAAWNNGDEHAKFYDDGTHEDKTA
                     GDNIWSVTLELVSDGGANSWEWGVNDVKGGWLYGNFKFTVADGKAQTLTHSNTVSAKN
                     FGLATCKVYPNPATDFISIEGKEFKSVEIYNIAGVKVLSSKAISQQIDISSLKQGSYI
                     VKALTTEGQNLISRFNKN"
BASE COUNT          386 a          329 c          295 g          522 t
ORIGIN      
        1 attctgggct tcgtcgagaa tgacgaaagc gttgctaaga gttcttcccc gcataaaggc
       61 taaaggcgct atttctatta ctttgtcttt catgaattct tccagttttt tctgaggaat
      121 catgtcgccc agcgcatcgt acaacggttg caggtagggg tcgattttct cctttaagtc
      181 gccgggtaaa aagcctaacc tttctccagc ttcaacggct ggccggctta aaataatctt
      241 tctgatttcc ctgtttttta acgctttcac agcaagtgca atagcggtgt atgtttttcc
      301 tgtacctgca gggccaatgg caaatatcaa atcattctca tcggtagctt caactaattt
      361 tcgttggttg ggggttctgg cccgaactgg tcttccgttg atgccaaata caagcacatc
      421 gctgttttct gtggtttgcc cttcaattgg agattgttcg gctactattt ggataatcac
      481 tttttcgttc agcgagtgga aatcgtggta gtaatcgagt agttcattga tttttttctc
      541 gaaatgaaag ctgtcttctt cactcccttt tactttaatc atatctcccc gggctattat
      601 tttcagcttt ggaaagagtg attttatcaa gtcaagttta gtattattta ctccgtaaaa
      661 ttcaaggaga tcaactcctt cgagatgtat tgatttttcg aacatgcttt attccctctt
      721 ttgctttgct acaaaaataa gcagattttc ggaaggcata aaaataaaaa aagccggcag
      781 tttagtaccg gctttctcat gtatttcaaa taactagtta ttagttcttg ttaaatctgc
      841 tgataagatt ttggccttca gtagttaacg ctttaacaat atagcttcct tgtttcaatg
      901 aagaaatatc gatttgctgg cttattgcct tgctgctcaa tacttttact cctgcaatat
      961 tgtaaatttc aactgatttg aattcttttc cttcaatact aatgaaatca gttgctgggt
     1021 taggatacac tttgcatgtg gctaatccaa agttctttgc tgatactgta tttgagtgag
     1081 tgagtgtctg tgcttttcca tcggcaacag tgaatttgaa gttaccgtaa agccaaccac
     1141 ccttaacatc gtttacaccc cattcccagc tgttcgcacc accatcagat actaattcaa
     1201 gggttacaga ccaaatgttg tcaccggcag ttttgtcttc atgcgttcca tcgtcataga
     1261 attttgcatg ttcgtcgcca ttgttccaag cggcatcaaa ttcgcctgtt gcagtgttcc
     1321 agcttccttt tagaccaaag cctgtgctgg attttagggc agagtcgtca acaacaaaag
     1381 tgatggctgc tttggccgga gcctgaactg cagaaacttt cccaatataa atataaccac
     1441 ctacaataga gttccaaccc gttgtttctt gtccggcaaa ctggaatata ccaattgtat
     1501 ctccagcagc aatagtgagt ttgtattcag ca
//
LOCUS       DAOPUW010000548         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_92779_length_1532_cov_2.183480, whole genome shotgun sequence.
ACCESSION   DAOPUW010000548 DAOPUW010000000
VERSION     DAOPUW010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92779_length_1532_cov_2.183480"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PKJ43_08565"
     CDS             <1..664
                     /locus_tag="PKJ43_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNZ72661.1"
                     /translation="RLQYLFYNFLMIRGLVLAERSRKKADSEVMQFAVDVRWLRFVVY
                     GYAIACLSGIVYLVFYFSGYEQAWVINLISISYFFLFFFIIFFNAITHDPTAADNKQK
                     QSQSPSLPMISLMKRIEELIVGQKLYLEPDLTLQQIAEGLNEKERNISQAINTIEKRN
                     VNDYINNFRIEHACTLLLGDKDKPVFEVMYESGFSTKGAFNLAFKRVTGKTPTQYRNE
                     VK"
     gene            808..1182
                     /locus_tag="PKJ43_08570"
     CDS             808..1182
                     /locus_tag="PKJ43_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNZ72662.1"
                     /translation="MQLTKAEEQIMQILWEMGEGMVRDIRDRFDEPKPARNTVSTIIR
                     VLEAKGIVSYRAEGNTHIYFPLVSKREYSKKQLFSLMKNYFDNSFPQMATFFAREKEL
                     TIDELEQLLNATKDELQNPNNQ"
     gene            1185..>1532
                     /locus_tag="PKJ43_08575"
     CDS             1185..>1532
                     /locus_tag="PKJ43_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72663.1"
                     /translation="MEAIGSYLLQMACWLAAFWLVYILALRKETFFELNRWFLLVGIM
                     AAFLLPLFPVRYDVIKNPTDLSLLLMTGKLSETTSAVKDPVNYWFLFYGLGAFVVFAR
                     FLLQCIKLLWLIRK"
BASE COUNT          445 a          282 c          330 g          475 t
ORIGIN      
        1 aaggcttcag tatttgtttt ataattttct gatgattagg ggcttggtac tagccgagcg
       61 cagtcggaaa aaagcagatt ccgaagttat gcaatttgcc gttgacgttc gttggttgcg
      121 ttttgtggta tacgggtatg ctattgcatg cttaagtggt attgtgtact tggttttcta
      181 tttctcaggt tatgagcagg cttgggtaat aaacctgatt agtataagct actttttcct
      241 gttctttttt atcatcttct tcaacgccat aacgcacgat cctacagctg ccgacaataa
      301 gcaaaagcag tcacaatcgc caagtttgcc aatgatctcg ttgatgaaaa gaattgaaga
      361 gttgattgtt ggccaaaaac tgtacctcga acccgacctt accctgcagc aaattgccga
      421 ggggctaaat gaaaaggaaa ggaatatttc tcaggcaatc aatacgatag aaaaacggaa
      481 cgtgaacgat tacatcaaca atttcaggat tgaacatgct tgtacccttt tgctgggcga
      541 caaagacaaa ccggtgtttg aagtaatgta cgaatcgggg tttagcacca agggagcttt
      601 caatctggcc tttaaaagag tgactggaaa aacacctacc caataccgta acgaagtaaa
      661 atagcggtca cttagttgta agatgttgaa attcaaatgt atgtgttttt gctgaaattt
      721 tttcccatat tgactaaaaa aatagtcatt tttcttgttt gactaaatcc ttagtcatat
      781 atttgttccg gacttacatt tacaattatg caattaacga aagctgaaga acaaataatg
      841 cagatacttt gggaaatggg cgaggggatg gttcgagaca tccgcgaccg cttcgatgaa
      901 cctaagcctg cacgcaatac tgtttctaca attattaggg tactcgaagc caagggaatc
      961 gtgagctacc gtgccgaagg gaatacacac atttattttc cactggtttc gaaacgtgaa
     1021 tattcgaaga agcaactttt ttcattaatg aagaattatt ttgataactc gtttccgcaa
     1081 atggcaactt tttttgcacg tgaaaaagaa cttaccatcg acgagttgga acagttgttg
     1141 aatgccacaa aagatgaatt gcaaaatcct aacaatcaat aacaatggaa gcaataggta
     1201 gttatttgtt gcaaatggct tgttggctgg ctgcgttttg gctggtgtac atactggcac
     1261 tgcgaaaaga aacgttcttt gagctcaacc gttggttttt gcttgttggc ataatggctg
     1321 catttctatt gccattattc cctgtaagat acgatgtgat taaaaatcca accgatttat
     1381 ctttgctttt gatgactggc aagctgtccg aaaccacctc agcggtaaaa gacccggtaa
     1441 actattggtt cttgttttat ggcttagggg ctttcgttgt atttgcccgt tttttgctgc
     1501 aatgcattaa gctattgtgg cttattcgca aa
//
LOCUS       DAOPUW010000549         1530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_92999_length_1530_cov_1.945763, whole genome shotgun sequence.
ACCESSION   DAOPUW010000549 DAOPUW010000000
VERSION     DAOPUW010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92999_length_1530_cov_1.945763"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1530)
                     /locus_tag="PKJ43_08580"
     CDS             complement(<1..>1530)
                     /locus_tag="PKJ43_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ72664.1"
                     /translation="LILGPLAKILETEQSPDLREKHLMIYRNASRLLQLTNRIMDFRK
                     NEKEQLKLNVEETDITEYIKNIFLFFNYEAQKRDITYQFSSTYNKKVLLDREFIESIV
                     FNMLSNAFKYTPDGKSIAVKICNDDKWVKVVFTDTGRGIATEQLDHIFDRFNSTSKRN
                     STGIGLSFSKRLIELHKGDIEVKSTFGEGSEFTIKIPLRDIYSHNEKVAVKENEHTVN
                     WAKIDATLQQDVAEDLKKLKSQYEKEELIALVVDDNFEVRQFISDLLQNDFKVIQASN
                     GKEALEIAFETIPDIVISDIMMDEIDGLELCSTLKKDERTDHIPVVLTTVLSAQADRV
                     KGLSVGADSYIPKPIDPAHLLIRVKKLIEKQLKLKDKFNLSSYDPSEDKEEAKPEEKV
                     HPLVEKARNIVLKNLDNSEYNIDDFCSDLGLSRMQLYRKFKAITGLSANSFIRKVRLH
                     KAAELLKTGELTVKEVTYDVGFIDLKYFRKCFFEEFGVNPSEYGQVREDNKEEQRQLF
                     PF"
BASE COUNT          347 a          322 c          343 g          518 t
ORIGIN      
        1 aaatgggaat aattgccgct gttcttcttt gttgtcttct ctgacttgcc catattccga
       61 tgggttgaca ccaaattcct cgaagaaaca tttcctgaaa tacttaaggt cgatgaaacc
      121 aacatcgtag gtgacttctt tcacggtcag ctcgccagtt ttaagcagtt ccgcggcttt
      181 gtgcaagcgt actttgcgta taaagctgtt ggccgacaag ccggtgattg ctttgaattt
      241 acggtatagt tgcatgcggc tcaagccaag atctgaacaa aaatcatcga tattatattc
      301 cgaattgtcg aggtttttga ggacaatatt gcgtgctttt tcaaccagtg ggtgaacttt
      361 ctcttcgggc ttggcttctt ctttatcctc cgaaggatcg tagctcgaca ggttgaattt
      421 atctttcagc ttaagctgtt tctcaattaa ctttttaacc cttatgagca ggtgtgccgg
      481 gtcgataggt tttggaatgt acgagtcggc accaacactg agccctttta cccggtcggc
      541 ttgtgccgat agtaccgtgg tcagcacaac agggatgtgg tcggtacgtt cgtctttttt
      601 caaggtcgaa catagctcta acccgtctat ctcgtccatc ataatgtcgg aaattacaat
      661 gtcggggatg gtttcaaaag caatttcgag tgcttctttt ccgtttgatg cctgaattac
      721 cttgaaatcg ttttgcagca ggtcgctgat aaactggcgg acttcaaaat tatcgtcgac
      781 aaccagtgca atgagttctt ctttttcata ttgcgacttc agttttttca ggtcttcggc
      841 cacatcttgc tgaagggtgg catcaatttt tgcccagttc acagtgtgtt cgttttcttt
      901 taccgcaact ttttcgttgt gcgaataaat atcgcggagc ggtattttta tagtgaattc
      961 agagccttct ccaaaggtgc ttttcacttc aatgtcgcct ttgtgaagtt cgatcagtcg
     1021 tttcgaaaag cttagcccga tgccggtgct gttacgtttt gaagtgctgt tgaaacggtc
     1081 gaaaatatga tcgagttgct cggtggcaat gccgcgcccg gtgtcagtaa agaccacttt
     1141 cacccattta tcgtcgttgc atattttcac tgcaatactt tttccgtcgg gcgtatattt
     1201 aaaggcgttc gacaacatat tgaacactat gctttcgata aattccctgt cgagcaatac
     1261 ttttttgtta tatgtgctcg agaattggta agtaatgtct cttttctgtg cttcgtagtt
     1321 gaagaacagg aatatatttt ttatgtattc ggtgatgtcg gtttcttcta cattaagctt
     1381 tagctgctct ttttcatttt ttctgaaatc cataatgcgg ttggttagct gcaacaaacg
     1441 gctggcatta cggtagatca tcaggtgttt ctcgcgcagg tcgggcgatt gttcggtttc
     1501 aagaatttta gccagtggcc caagaatcaa
//
LOCUS       DAOPUW010000550         1529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_93047_length_1529_cov_3.006106, whole genome shotgun sequence.
ACCESSION   DAOPUW010000550 DAOPUW010000000
VERSION     DAOPUW010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93047_length_1529_cov_3.006106"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..>1529
                     /locus_tag="PKJ43_08585"
     CDS             15..>1529
                     /locus_tag="PKJ43_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNZ72665.1"
                     /translation="MFKASAGSGKTFRLVVEYLKLLIKNPNNYKHILAVTFTNKATTE
                     MKERILRDLYSLSRQENKQLLQLLMNECRVSAQDVELNSKRALSLILHDYDRFAVSTI
                     DSFFQGILRSFARESGLYGSYEIDLDMDAVLAEACDRMLMSIDKDTELREWLTQMAEN
                     RLQEEKGWQIKDTILSVGKELTKSVFVDYRLHQGSLTEERENIKELKKTLQTIKKQYE
                     AECRGVGKEGLALMEKHNLSFDDFKYKGTSFPKMFEKLANFKGGELKMAKRFEDAPDN
                     VESWYGDKKNTSRIDAIYHDGMNQLVKEFLDYYEKNWTRYLTAKAIYSNIFTLGVLST
                     LSQFVREVGQENNSLLLSESDNLLRGIIAGNDAPFIYEKAGNYYQFFMIDEFQDTSIV
                     QWDNFKPLITNSLAENNPNLVVGDVKQSIYRWRNSDWKLLNSNIKDDLKNYTLSEENL
                     NSNWRSRTNIVEFNNWFFPISAAFLQDAYNQANISSNLYQSTIIDVYADVQQIAA"
BASE COUNT          498 a          276 c          320 g          435 t
ORIGIN      
        1 tgtctgaaat taaaatcttt aaagcatctg ctggttctgg aaaaaccttt cgtttggtag
       61 tcgagtattt aaaattacta atcaagaacc caaacaatta taaacatatc cttgctgtta
      121 catttaccaa caaggcaact accgaaatga aagaacgtat tctgcgtgat ttatattcat
      181 tgtcacggca ggagaacaag caactattac agttgctgat gaacgaatgc cgcgtttcag
      241 cccaagatgt ggagttgaac tcgaagcgtg cactttcact tattttacac gactacgatc
      301 gctttgctgt aagtaccatc gatagttttt ttcagggaat tctgcgctca tttgcgcgcg
      361 aatcgggctt gtatggttct tatgaaatcg atctcgatat ggatgctgtt ttagccgagg
      421 cttgtgaccg tatgcttatg agtattgata aagatactga actgcgcgaa tggctaaccc
      481 aaatggcaga aaaccgcttg caggaagaaa agggttggca aataaaagat accatcttgt
      541 cggttggtaa agagcttact aagagtgttt ttgttgatta caggcttcat cagggatcat
      601 taaccgaaga acgggaaaac atcaaggaac taaaaaaaac actgcaaaca ataaaaaagc
      661 agtatgaagc agagtgccga ggggttggaa aagagggact tgctctcatg gaaaagcata
      721 acctttcgtt tgatgatttt aaatacaaag gaacctcatt ccctaaaatg tttgaaaagc
      781 ttgccaactt caagggtggt gagttaaaaa tggcaaaacg tttcgaagat gctccagata
      841 acgtagaatc atggtatggc gacaaaaaga atacctccag gattgatgca atttatcacg
      901 atggaatgaa ccaactcgtt aaggagttcc ttgattatta tgaaaaaaat tggacaagat
      961 atcttacggc taaagcaatt tattcaaata ttttcacctt aggggtgttg tctaccttat
     1021 cgcagtttgt gcgtgaagtg gggcaagaaa acaattcttt gctgttaagc gaaagcgaca
     1081 accttttgcg gggtataatt gcaggaaacg atgctccgtt tatttatgaa aaagcaggca
     1141 attattatca gtttttcatg attgatgaat ttcaggatac ctcaatagtt cagtgggata
     1201 attttaagcc tttgattacg aactcgctgg ctgagaataa ccccaatctt gttgtaggag
     1261 atgtaaaaca atcaatatac cgttggcgca acagcgattg gaaattactc aatagcaaca
     1321 tcaaagacga tttgaaaaat tacacccttt cagaagaaaa ccttaacagc aactggcgga
     1381 gccgaaccaa tattgttgaa ttcaataatt ggtttttccc tatatctgcc gcctttttac
     1441 aagacgcata taatcaggcg aatatttcct cgaatctata tcagtctact attattgatg
     1501 tgtatgccga cgtgcagcaa attgcagca
//
LOCUS       DAOPUW010000551         1528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_93133_length_1528_cov_3.865580, whole genome shotgun sequence.
ACCESSION   DAOPUW010000551 DAOPUW010000000
VERSION     DAOPUW010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93133_length_1528_cov_3.865580"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1528)
                     /locus_tag="PKJ43_08590"
     CDS             complement(<1..>1528)
                     /locus_tag="PKJ43_08590"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNZ72666.1"
                     /translation="GVLSNLPGDTKPVSVIEDTAIPVARFTEFLEEFRNMMKEMNLEA
                     VYHGHIATGELHLRPMLNLKDKTDVERFRAVATRTAILVKKYRGSLSGEHGDGRLRGE
                     FIPLMLGERVYQLLKEIKACWDPNQIFNPGKITNTPAMNQSLRFEPGNETRTLNSYFE
                     YKLEPGMARAIEKCNGSGDCLKQQARGGTMCPSYMATRDEMHSTRGRANILRELITRP
                     QKLNWADQHEVLYALDLCLSCKACKSECPSGVDITKLKADFLQNHYDANPIPFRSKII
                     ANFPSIMNLAAFFRPVSNFAMRSHILSPLIKLAIGFAPQRPLPQLSKIRMSGWYAKQA
                     KHQGLEKGKVYLYIDEFLELTDSHIGIKAVLLLEKLGYEVIIPPHVASGRTYLSKGFV
                     KKARNLAEKNIEMLKDIISPETPLIGIEPSAILSFRDEYPEIVGEKYKNDARKLAESV
                     LMFDEFIVRELEKGNISREQFTSEKAEIKLHGHCHQKSLATTSSSIKMLQIPINYTVT
                     E"
BASE COUNT          368 a          317 c          356 g          487 t
ORIGIN      
        1 tctctgtaac cgtatagtta atgggtattt gcagcatttt tattgaagat gaagttgttg
       61 ctaacgactt ttgatgacaa tggccatgta atttaatttc agctttttca ctagtaaatt
      121 gttctcggct gatgttacct ttttctaatt cacgaacaat aaattcatcg aacattagca
      181 ccgattcggc taatttgcgg gcatcgtttt tgtatttttc gccaacaatt tcggggtatt
      241 catcgcgaaa tgacaagatc gcagaaggct caattccaat aagcggggtc tcaggactga
      301 taatgtcttt taacatctca atattctttt cggctaaatt ccgggctttt tttacaaatc
      361 ctttcgaaag ataggttcgt ccactggcta cgtggggcgg tattatcact tcgtatccaa
      421 gtttttcgag aagaagaacg gcttttatac ctatatgaga gtcggttagt tcaaggaatt
      481 catcgatata gaggtaaact tttccttttt caagcccttg atgttttgct tgtttagcgt
      541 accagcccga catacgtatc tttgaaagtt gaggcagagg tcgttgcggt gcaaacccaa
      601 ttgcaagttt aatcaagggt gaaagaatat gcgaacgcat ggcaaagtta gatacaggac
      661 ggaagaatgc ggccaaattc atgatggaag ggaagttggc tataattttc gacctgaagg
      721 ggataggatt tgcatcgtaa tgattttgca gaaagtcggc tttaagcttg gtaatatcca
      781 ctcccgatgg gcattcgctt ttgcatgctt tgcacgagag gcataggtcg agggcgtaaa
      841 gcacttcatg ttgatcggcc caattgagct tttgcggccg ggtaattaat tcccgcaaaa
      901 tatttgccct gcctcgtgtt gaatgcattt cgtctcgggt agccatataa ctggggcaca
      961 tggtacctcc gcgagcttgt tgtttcaggc aatcgccaga gccattacat ttttctattg
     1021 cacgagccat tccgggctct aatttatatt caaagtagct gttcagtgtt cgggtttcat
     1081 ttcccggctc aaagcgcaac gattggttca ttgccggggt gttggttatt tttcccggat
     1141 tgaatatttg gttgggatcc cagcatgctt ttatttcttt taacagttga tacacccttt
     1201 ctcccagcat caatggaatg aattctccgc ggaggcgacc gtcgccatgc tcaccactaa
     1261 gcgaaccgcg gtattttttt accaaaattg cagtccgtgt ggctacagcc ctgaagcgct
     1321 ctacatcggt tttgtctttt aggttgagca ttggcctcag gtgcaactcg ccggtggcaa
     1381 tgtgtccgtg gtaaacagct tcgaggttca tttccttcat catgttcctg aattcttcga
     1441 gaaattcggt gaatctggct acaggaatag ctgtgtcttc gattactgaa acgggttttg
     1501 tgtcgccggg caaatttgat aggacccc
//
LOCUS       DAOPUW010000552         1528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_93149_length_1528_cov_3.138493, whole genome shotgun sequence.
ACCESSION   DAOPUW010000552 DAOPUW010000000
VERSION     DAOPUW010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93149_length_1528_cov_3.138493"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /gene="hisB"
                     /locus_tag="PKJ43_08595"
     CDS             <1..957
                     /gene="hisB"
                     /locus_tag="PKJ43_08595"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HNZ72667.1"
                     /translation="QDGLGTASFPEETFWPAHNKMLKAFEGEGVVFNDILIDRSFPED
                     NAPTRKPRTGLMGKYMDGSYDLANSYVIGDRLTDVELAKNLGCKAIWLSDQPTELLAE
                     KGLTEVCVLKTTNWDEVYAEVAKTDRTASVRRTTKETDIDVWLDLDGTGKTNISTGLG
                     FFDHMLDQIGRHSGIDLKVIVKGDLEVDEHHTIEDTALALGEAFLKALGNKRGIERYG
                     FCLPMDDCLAQVAIDFGGRPWLVWDAEFKRERVGDMPTEMFLHFFKSFSDASKCNLNI
                     KAEGQNEHHKIEGIFKALAKSIKMAIRRDVFNFELPSTKGVL"
     gene            988..1359
                     /locus_tag="PKJ43_08600"
     CDS             988..1359
                     /locus_tag="PKJ43_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNZ72668.1"
                     /translation="MDREGIFKKVLDCAFEVHSNLGPGLLESVYEECLIFELQNAGLK
                     VEKQKNLPLTYKGLLLESRLRIDILVENQIIIELKAVEELTDVHLAQTLTYLKLSGCR
                     LALLVNFNVTHFKNGIKRVIM"
BASE COUNT          486 a          324 c          338 g          380 t
ORIGIN      
        1 caggatggct tgggaacagc ttcgtttccc gaagaaacct tttggcctgc tcacaacaaa
       61 atgctgaaag cgtttgaggg cgaaggcgtg gtattcaacg acattctcat cgaccgcagc
      121 tttcctgagg acaacgctcc aacccgcaag cctcgtaccg gattgatggg caaatacatg
      181 gacggcagct acgacctggc caacagttac gtcattggcg accgccttac cgatgtagaa
      241 ctggcaaaaa atctcggttg caaagccatt tggctcagcg atcagcccac agaacttctt
      301 gctgaaaaag ggttgactga agtttgtgtg ctgaaaacca ccaattggga cgaggtatat
      361 gccgaagtgg ccaaaacaga tcgcaccgca tcagttcgcc gtaccaccaa agaaaccgac
      421 atcgacgtat ggctcgacct cgacggaacg ggtaaaacca acatttcaac aggattgggc
      481 tttttcgacc acatgctcga ccaaattggc cgtcactcgg gcatcgacct gaaagtgatt
      541 gtaaaaggcg acctagaggt tgacgaacac cataccattg aagatacagc attggctctt
      601 ggcgaggctt ttctgaaagc actgggcaac aaacgaggca ttgagcgtta cggtttctgc
      661 ttaccaatgg acgattgtct agcgcaggta gctatcgact ttggtggccg tccttggttg
      721 gtatgggatg ccgaattcaa gcgcgaacgt gttggcgaca tgcccaccga gatgttcctg
      781 cactttttca agtcgttcag cgatgcgtcg aagtgcaacc tcaatatcaa agctgagggg
      841 caaaacgagc accacaaaat tgaaggcatc ttcaaggcat tggcaaaatc gatcaaaatg
      901 gcaatacgaa gagacgtttt caacttcgaa ctgccaagca ccaaaggagt tttataaaca
      961 caaagaaaat attcaaatta aaggaatatg gatcgtgaag gaatattcaa aaaggtgtta
     1021 gactgtgctt ttgaagtgca ttcgaacctt ggtccaggtt tattggaaag tgtttacgaa
     1081 gaatgcctta tttttgagct tcaaaatgct ggacttaaag ttgaaaaaca aaaaaatctg
     1141 cctttgacat ataaagggtt gcttctagaa agccgcctca gaattgatat tcttgttgaa
     1201 aatcaaatca taattgaatt gaaagctgta gaggagttaa ccgatgtgca tttagctcaa
     1261 accttaactt acttaaaatt atcaggttgc agacttgcac ttttagtaaa cttcaatgta
     1321 acacatttta aaaacggaat taaacgagta attatgtaag aggaaacact aaggaaataa
     1381 ggatgccact atgaacacta aaatttaaaa ttacaaaaat tgaaattctt tgtgccctca
     1441 gtgaaaaacc ttcgtagcct ttgtggttat aaaacataca actatgacta tagacaaaag
     1501 caaatcaact gctattcttc ttattcac
//
LOCUS       DAOPUW010000553         1527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_93293_length_1527_cov_2.380435, whole genome shotgun sequence.
ACCESSION   DAOPUW010000553 DAOPUW010000000
VERSION     DAOPUW010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93293_length_1527_cov_2.380435"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1082)
                     /locus_tag="PKJ43_08605"
     CDS             complement(75..1082)
                     /locus_tag="PKJ43_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72669.1"
                     /translation="MKKLSLFALILFFSCRVFAQTVDYASHFNGIGDNREFFSNYNKP
                     QTILGTRASFTLGGKIDSVHQVRVGIDYFYEYGSKFGELPPHMILYYQFLNSKWHFRM
                     GTFARKEIMDLPLAIIADDWDYFNPNLEGLFVGYRNNSINVNAYLDWISRQSNTRREQ
                     FMAGVNGDAHFKKITFSFNGYMFHNAKTALWNENTFIEDYMGGLVLLGYDFSSILPLD
                     VAYIKTGILNSLARNRGDGMDFRVANSSYSQFQLEENGIGLRGTFNVGDKHSFYFGDS
                     FYSNTTSYHRIDLYVTPLNFKNVKGRLGWSFHIANGDLDNQQQFSLLYTFGGDLGKNN
                     K"
     gene            complement(1174..>1527)
                     /locus_tag="PKJ43_08610"
     CDS             complement(1174..>1527)
                     /locus_tag="PKJ43_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72670.1"
                     /translation="EKFTSVEFIYGEKNSGRMEPYHRMDIGLVHNTKTKRGNRATWTY
                     SIYNVYNRINPYNYYYDNDNDRRNLTYTNRPLKLYKIGLFAFMPSISYKVYFDYSKRE
                     KQQKKEKKNWLYFDE"
BASE COUNT          472 a          314 c          273 g          468 t
ORIGIN      
        1 aaaaattgaa agcgggaagg ttcttcccac aggaatttaa gcatgtttgc cgtataggct
       61 tgtttgtaaa atgcttattt gttgttcttt cccaaatcgc caccaaaagt ataaagcaga
      121 gaaaattgct gttggttatc gagatcgccg ttagcaatgt gaaacgacca tcccaggcgc
      181 cctttcacat tcttaaaatt gagtggtgtt acataaagat cgattctgtg atagctggtt
      241 gtgttggagt aaaacgaatc gccaaaataa aaagaatgct tgtcacccac attaaaagtt
      301 ccgcgtaacc cgataccatt ttcctcaagc tgaaactgag aatatgaact gtttgccacg
      361 cggaaatcca ttccatctcc acggtttcgg gccaaagaat tcaaaattcc tgtttttata
      421 tacgcaacat cgagaggtaa aatgctggaa aaatcatatc caagcaacac aagtccaccc
      481 atataatctt caataaacgt gttttcattc caaagggctg tttttgcatt gtggaacatg
      541 tacccattga aagagaaagt tatctttttg aaatgcgcat caccattaac tcctgccata
      601 aattgctccc tccgggtatt gctttgcctg ctaatccaat ctaaataagc atttacgttt
      661 attgaattgt tgcggtaacc cacaaaaagc ccctcaaggt tcgggttgaa gtagtcccag
      721 tcgtcggcaa taattgctaa gggcaaatcc attatttctt tccgggcaaa agttcccata
      781 cggaaatgcc actttgagtt caaaaattgg taataaagga tcatatgagg aggcaattcg
      841 ccaaacttgc ttccatactc gtaaaaataa tcgatgccaa cccgaacctg atgtaccgag
      901 tcgattttgc cgcctaaagt gaaagatgcc ctagttccaa gaatagtctg aggtttgtta
      961 tagttgctaa aaaactcccg gttatcgcca attccattga aatgagatgc atagtcgacc
     1021 gtttgtgcaa aaacacggca cgaaaaaaac aatatcaatg caaaaagaga aagcttcttc
     1081 atctcaattt atttgttggc ataatacaat gccaaatgta gaaaacaaaa atgaaactca
     1141 ctcattgatg gaaactttct gtaactggct aaattattcg tcgaaatata accaattttt
     1201 cttttccttc ttctgctgtt tttctctttt cgaataatcg aaatacactt tgtatgaaat
     1261 gcttggcatg aaagcgaaca acccaatctt atacagtttt aatggccggt tagtatatgt
     1321 caaattccgg cgatcgttgt cgttgtcgta gtaataattg tatggattaa tcctgttgta
     1381 cacgttataa attgaatatg tccatgtagc tctatttcct cttttggttt ttgtgttgtg
     1441 caccaaacca atatccatcc tatgatatgg ctccattctt cctgaattct tttcaccata
     1501 aatgaactca accgatgtga atttttc
//
LOCUS       DAOPUW010000554         1523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_93709_length_1523_cov_2.294278, whole genome shotgun sequence.
ACCESSION   DAOPUW010000554 DAOPUW010000000
VERSION     DAOPUW010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93709_length_1523_cov_2.294278"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1523)
                     /gene="feoB"
                     /locus_tag="PKJ43_08615"
     CDS             complement(<1..>1523)
                     /gene="feoB"
                     /locus_tag="PKJ43_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNZ72671.1"
                     /translation="LRETVHRKAKTINVALVGNPNCGKTTLFNYATGRHEKVGNYGGV
                     TVDFKTAQLQHNGYEINFTDLPGTYSISEYSPEELYVRKHLSNELPDVVVNVVDASNL
                     ERNLYLTTQLIDMNIKVVIALNMYDELTASGIEFRYKELARMIGVPIVPTIASKGKGI
                     NELLDEIIAIDEEREPLFRHIHISYGSHINLAIEKLSVEIKKVKKITDKYHTHYVALK
                     LLEGDTLFLKEIESITGSSVIIEFANKERNKLEKEFNEKASSLIADAKYSFIRGALKE
                     TCKQGNRMVPKNNYRPDKILTNKWLGFPVFLLLMWAMFQSTFTLGSYPVEWIDSGVNW
                     LGNMLKNSMAEGPLRDLLVDGIVGGVGGVIVFLPNILILFFFISLMEDTGYMARAAFI
                     MDKLMHKIGLHGKSFIPMLMGFGCNVPAIMATRTLESRKDRLLTMMIIPFMSCSARLP
                     VYVLLISAFFPQRQGLILLAIYMIGVLVAVFMALIFNKLFFSKKEAPFVMELPPYRI"
BASE COUNT          444 a          339 c          301 g          439 t
ORIGIN      
        1 gaatacgata aggcggaagc tccataacaa agggtgcttc tttcttcgag aaaaacaatt
       61 tattaaaaat cagcgccata aacacagcaa ccaatacccc tatcatatat attgccagaa
      121 gaataagacc ttgtcgctgt ggaaagaagg cagaaataag cagcacataa accggcaaac
      181 gggcactaca cgacatgaac ggaatgatca tcattgtaag caaacggtct ttccggcttt
      241 cgagtgtacg agtagccatt atggccggaa cattgcatcc aaagcccatc aacattggaa
      301 taaacgattt tccgtgtagc ccgattttat gcataagctt gtccattata aaagcagcac
      361 gggccatgta accagtatct tccatcaacg agatgaaaaa gaacaagata agtatgttag
      421 gtaaaaaaac aattactcca ccgaccccac caacaatacc gtcaactagc aagtcgcgca
      481 atgggccttc tgccattgag tttttcagca tattgccaag ccagtttacc ccagaatcga
      541 tccattcaac agggtaactt cccaatgtga aagttgattg gaacatggcc cacatcaaaa
      601 gcagaaaaac cgggaaacca agccatttat tggttaagat tttatcggga cggtaattat
      661 ttttggggac catacggttt ccctgcttac atgtttcttt gagcgcgcca cgaataaacg
      721 agtattttgc atcggcaatt aatgaagaag ctttctcgtt gaattccttc tcaagtttat
      781 ttcgttcttt attagcaaat tctattatga cactactccc tgtaatgctt tcaatttctt
      841 tcaagaaaag ggtgtcgccc tcgagcagtt tcaatgccac gtaatgggtg tgatatttat
      901 cggttatttt ttttactttt ttaatttcaa ccgataattt ctcaatcgcc aggtttatgt
      961 gcgatccgta actaatgtgt atatggcgaa acagcggttc ccgttcctca tcgatagcaa
     1021 ttatttcatc aagcaactcg ttgattcctt tgcccttcga agcaatagtt ggaacaatag
     1081 ggacaccaat catgcgggcc agttctttgt accgaaattc gattcccgag gctgtaagct
     1141 catcgtacat attcagagca ataaccactt taatgttcat gtcgatgagc tgtgtggtaa
     1201 ggtacaggtt gcgctcaaga ttcgatgcat cgaccacgtt aaccaccaca tcgggtaatt
     1261 cgttactaag gtgttttctc acatagagtt cctcggggct atactccgat attgaatagg
     1321 tgcccggcaa atcggtaaaa ttaatttcgt agccattatg ctgtagctgg gctgttttaa
     1381 agtcgacagt aactcctcca taattgccaa ctttttcgtg ccgccctgtt gcatagttga
     1441 aaagagtagt tttcccgcaa ttggggtttc ctaccaaagc tacatttatt gtttttgcct
     1501 ttcggtgaac ggtttcacgc aac
//
LOCUS       DAOPUW010000555         1520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_93986_length_1520_cov_2.697611, whole genome shotgun sequence.
ACCESSION   DAOPUW010000555 DAOPUW010000000
VERSION     DAOPUW010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93986_length_1520_cov_2.697611"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PKJ43_08620"
     CDS             <1..615
                     /locus_tag="PKJ43_08620"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ72672.1"
                     /translation="NVQIVTEAQFYTQLENNAVGCNLCPRNCIIEAGKFGNCKARRNR
                     NGTLISEVYGKIAALNSDPVEKKPLYHFFPGNEILSIGTTGCNLHCVFCQNFALSQCD
                     IRQPVSVKGFTPEELAALSLKKKNNLGVAFTYNEPTVNYEFMIRTAELVKQNNQKTVV
                     VSNGYINPDPLKILLKNIDAFNIDLKAFDDNFYKKYSKASLDPV"
     gene            712..1020
                     /locus_tag="PKJ43_08625"
     CDS             712..1020
                     /locus_tag="PKJ43_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72673.1"
                     /translation="MCDWIAKETGRDTVLHLSRFFPRYELDQYPTPPETLFTLFDIAR
                     EKLNYVYIGNMATELHSSTYCPTCKTLLIERTYYNTRKHAISPEGKCTKCGKLVINEI
                     "
     gene            1120..>1520
                     /gene="amrB"
                     /locus_tag="PKJ43_08630"
     CDS             1120..>1520
                     /gene="amrB"
                     /locus_tag="PKJ43_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNZ72674.1"
                     /translation="MEMKIRKAAFAGRFYPDDPKLIHAYMEHFLEKEQNKIKTMPESS
                     VIIGGVVPHAGIMYSGYEAVHFYEIVRRSHQRFDTVVIVNPNHTGNGIGAYNTATYSH
                     WETPLGTLENDTDFQDALNLPAYNLAHDFEHS"
BASE COUNT          503 a          289 c          284 g          444 t
ORIGIN      
        1 aacgtacaaa tcgtgaccga agcccaattt tatacacaac ttgaaaataa tgctgttgga
       61 tgcaatcttt gtccacgcaa ttgcataatt gaagctggaa aatttggtaa ctgtaaagcc
      121 cgcagaaacc gaaacggtac tcttataagt gaagtatatg ggaaaattgc cgcattaaac
      181 agtgatcctg tagaaaaaaa accattatac cattttttcc cggggaatga aatcctttcg
      241 attggaacaa ccggttgtaa cttacattgc gttttctgtc agaactttgc gctttcacag
      301 tgcgatatca gacaacctgt aagtgttaaa ggttttactc cagaagaatt ggcagcattg
      361 tcacttaaaa agaaaaataa tcttggcgtg gcttttacat acaacgaacc cactgttaat
      421 tacgagttta tgattcgtac tgccgaatta gtcaagcaaa acaatcagaa aacggtggtg
      481 gtaagcaacg gctatatcaa tcccgatcct ttaaagatcc ttcttaaaaa tatcgatgcg
      541 tttaatattg atttaaaggc ttttgatgat aatttttata agaaatattc caaagctagc
      601 ctcgatcctg tgtaaaatgc aattaaaatt attgctaaaa gtggtaagca ttttgaaatt
      661 accaatctgg ttattcctac gctcaatgat gatgaatttg agttcgccga aatgtgcgat
      721 tggatcgcta aggagactgg tcgagacacg gtgttgcact tatcccgttt ttttcctcga
      781 tacgaacttg accaatatcc aacacctcct gaaacacttt tcaccctttt cgacattgcc
      841 cgggaaaaac ttaattatgt gtatattgga aacatggcaa cagaattgca tagcagcacc
      901 tattgcccta cctgtaagac acttctcatt gagagaacat attataacac tcgtaaacac
      961 gctattagcc ccgaagggaa atgcaccaaa tgtggaaaat tggttattaa tgaaatttga
     1021 attttgatgt tcaaatactt tatttactta atcgaaaggc aattaaaaca ataatagcat
     1081 ttgtaaatgc ctgtataaat agcataatga tattcagtca tggaaatgaa aatcagaaaa
     1141 gcagcatttg cgggccgttt ctatcccgac gatccgaaat tgatacatgc ttacatggag
     1201 cactttcttg agaaagagca aaacaaaatt aagacgatgc cagaaagttc agtaatcatt
     1261 ggaggtgttg tacctcatgc cggcataatg tactcagggt atgaagctgt tcatttttac
     1321 gaaattgttc gccgaagtca ccagagattc gatacagttg taattgtaaa ccccaaccac
     1381 actgggaatg ggataggtgc ctataataca gcaacatatt cccattggga aactccgctt
     1441 ggtacacttg aaaatgatac cgattttcag gatgcgttga atttacccgc atataattta
     1501 gctcacgatt ttgaacattc
//
LOCUS       DAOPUW010000556         1506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_95321_length_1506_cov_3.458994, whole genome shotgun sequence.
ACCESSION   DAOPUW010000556 DAOPUW010000000
VERSION     DAOPUW010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95321_length_1506_cov_3.458994"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PKJ43_08635"
     CDS             complement(<1..219)
                     /locus_tag="PKJ43_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010421765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNZ72675.1"
                     /translation="MIILIKAAQFFLSLSILVIIHEFGHFIFAKIFKCRVEKFYLFFD
                     IGFRLFRTKRGETEYGVGWLPLGGYVKIA"
     gene            complement(233..1390)
                     /locus_tag="PKJ43_08640"
     CDS             complement(233..1390)
                     /locus_tag="PKJ43_08640"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010421766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNZ72676.1"
                     /translation="MTKRLAILGSTGSIGQQCLEIVDAHPNLFEVEVLTANNNAQLLI
                     NQALKYHPSKVIIANSDKYAQVKSALEPAKIQVFSGNEAIEDCVSDPSVDMVVAAMVG
                     YAGLIPVINAIKAKKQVALANKETLVVAGDLITRLCDEYSVDLLPVDSEHSAIFQCLQ
                     GENHASIEKLILTCSGGPFRGYSADDLSKVTPAQALAHPNWDMGNKITIDSATLMNKG
                     FEVIEARWLFNIEPSKIEVVIHPQSIIHSLVQFCDGSIKAQIGLPDMRLPIQYAMCFP
                     QRKPNTFPRFSFLEHPQLSFMAPDIKIFRNLALAYFALEKGGNMACIMNAANEVAVQA
                     FLDCKIGFQAIAEIIEQTMAKSTFIMAPAIEDYIQTDIEARAIAIKLISKK"
BASE COUNT          462 a          310 c          287 g          447 t
ORIGIN      
        1 tgcaatcttc acgtatccac ccaacggaag ccaacctact ccatattcgg tttctcccct
       61 tttagtccga aataatcgaa acccgatatc gaaaaacaga tagaactttt cgacacggca
      121 cttaaatatt ttggcaaaaa taaaatgacc aaattcgtga ataattacca gtatggataa
      181 actgagaaaa aattgtgcgg ctttaattaa tattatcata attaataatt ttctattttt
      241 ttgatatcag ctttatggca atagcccttg cttcaatatc ggtttgaata taatcttcaa
      301 tagcaggagc cataataaaa gttgattttg ccattgtttg ttcaattatt tctgcaatcg
      361 cttgaaaacc aattttgcaa tcgagaaatg cttgaactgc aacttcattg gcagcattca
      421 ttatacaggc catatttccg cctttttcga gggcaaaata agccaatgca agattacgaa
      481 aaattttaat gtcgggtgcc ataaaagaca actgtggatg ttcgaggaaa gaaaagcggg
      541 gaaaagtatt gggtttccgt tgcgggaaac acatggcata ctgaatagga agccgcatat
      601 cgggcaaccc aatctgtgcc tttatcgaac catcgcaaaa ctgaacaaga gaatgaataa
      661 tgctctgagg atgaataaca acctcaattt tggagggttc gatattgaaa agccatcggg
      721 cctcaatcac ttcaaaccct ttgttcataa gcgttgccga atcgatggtt attttgttcc
      781 ccatatccca attgggatgg gccagggctt gtgccggggt aactttactg aggtcatccg
      841 cagaatatcc cctgaacgga ccgcccgagc atgtcaatat cagtttttcg atagacgcat
      901 gattttcacc ttgcaaacac tgaaaaattg cagaatgttc agaatcaaca ggaagaaggt
      961 ctaccgagta ttcgtcacac aaacgggtaa tgagatcgcc agctacgacg agtgtttctt
     1021 tgtttgctaa tgcaacctgt ttttttgctt ttatggcgtt tattacagga attaaaccgg
     1081 catacccaac catagctgca accaccatat cgaccgaagg atcggataca cagtcttcaa
     1141 tggcctcgtt tccagaaaag acctgaattt ttgcaggctc aagcgcgctc tttacctgtg
     1201 catatttatc actatttgca atgattacct tagagggatg atatttcaac gcttgattga
     1261 taagtaactg tgcattatta tttgctgtga gcacttctac ctcaaataag ttgggatgag
     1321 catctacaat ttcgaggcat tgttgtccta ttgagccagt agagcctaaa atagccaatc
     1381 gtttcgtcat aagattcttt ttgctttgaa tgaaatattc aacacctgca tgctatgttt
     1441 tccaacgaac accgtaccga aacgaattgc cagcaatggt agtttcatta tatcaaaatt
     1501 ttatat
//
LOCUS       DAOPUW010000557         1501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_95879_length_1501_cov_2.059474, whole genome shotgun sequence.
ACCESSION   DAOPUW010000557 DAOPUW010000000
VERSION     DAOPUW010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95879_length_1501_cov_2.059474"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PKJ43_08645"
     CDS             <1..263
                     /locus_tag="PKJ43_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72677.1"
                     /translation="KKPSVITKWLSGTHNFESNTLFELEEKLGITLVALEEKKYAPTG
                     YYQVTVQSIVNSNQTDEGKYNIPSLFIYGDSFKTNNLKTYGC"
     gene            253..675
                     /locus_tag="PKJ43_08650"
     CDS             253..675
                     /locus_tag="PKJ43_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72678.1"
                     /translation="MGAKEIKFQLAGIEILEVNLKHPEAALPVPRKYNFETKIKTALN
                     PDQKQFHVMPDVTVIYDDDKSVHASIKVAFTFGIDNFEDFKSGEDVDFPDQFLVSMVS
                     ISISTLRGIMYSQFRGTFLNNTILPIIDPKSFVKETEE"
     gene            complement(835..>1501)
                     /locus_tag="PKJ43_08655"
     CDS             complement(835..>1501)
                     /locus_tag="PKJ43_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72679.1"
                     /translation="NKILAQGALTLMIAPTATSAPVIANMLGANINTMVTSTLLSNLV
                     VAAVSPLYFSWAGMHGDLPFWQSSWMVLSKVAPLLIAPLILAYLTQRYAPKLSEKISK
                     NKNASYYLWAISLTVVIGRTIDSIFLLDSSELGILLAMTILSIVLCVVQFGVGRITGK
                     IYGETIAGGQSAGQKNTVLAIWMAQTYMTPISSVIPAMYVLWQNLFNSYQIWNKNRRE
                     HRR"
BASE COUNT          457 a          327 c          307 g          410 t
ORIGIN      
        1 ataaaaaacc atcggtcata acaaaatggc tgagcggcac gcataatttt gagtcgaaca
       61 ccctttttga attggaggaa aaactgggca tcactcttgt tgccctcgaa gagaaaaagt
      121 acgcaccaac cggttactat caagttacgg ttcaaagcat agttaattca aatcaaaccg
      181 acgaagggaa atacaatatc ccatcgttgt tcatttatgg tgatagtttt aaaaccaata
      241 acttaaaaac ttatgggtgc taaagaaatc aaatttcaac ttgcgggaat tgaaatactg
      301 gaagtcaacc tgaaacatcc cgaagcagca ctacccgtgc cacgcaagta taatttcgaa
      361 acaaaaatca aaacagctct aaaccctgat caaaaacaat ttcatgtaat gcccgatgtc
      421 accgtaatat acgatgatga taaatcggta catgcttcaa ttaaggtagc ttttactttt
      481 ggaatcgaca attttgaaga ctttaaatca ggtgaggacg ttgattttcc cgatcaattt
      541 ttagtgtcaa tggtgtcaat atccatctct acacttcgtg ggatcatgta ttcacaattc
      601 aggggaacct ttctaaataa taccattctg cctattatcg acccaaaaag ttttgtgaaa
      661 gaaaccgagg aataaaccca ataccaccgc tcccgcacga gtcccctcga gtgggactct
      721 gctactgcgc gattttatcg tatttgtttc aatgaaaatt taattgacca tgcgattttc
      781 aaccgagtgg cagtgaaggg cttgccaatc tttcaaaaga tgccccaagt ttccttatct
      841 tcggtgttct cttcggtttt tgttccaaat ttggtaactg ttgaataggt tttgccacaa
      901 tacatacatg gccgggatta ccgatgaaat gggcgtcatg taggtttgtg ccatccaaat
      961 agctaaaacc gtgttttttt gtccagccga ctgtcctccg gcgatggttt cgccatatat
     1021 tttgccagta atgcgcccaa ctccaaactg aacaacacaa agcactatag atagaatcgt
     1081 catggccagc aaaataccca attctgaact gtcgagcagg aaaattgaat cgatagtacg
     1141 cccgattacc actgttaggc ttattgccca cagataatac gacgcatttt tatttttcga
     1201 aatcttttcc gataacttag gggcatatcg ttgtgtgaga taagccaata tcagcggggc
     1261 aatcagcagc ggggcaactt tcgagagaac catccagctc gattgccaaa agggtaaatc
     1321 gccatgcata ccggcccagg aaaaataaag cggagatacg gcagcaacca caagattcga
     1381 caatagcgtg ctggttacca tggtgtttat gttggctccc agcatgttgg caattaccgg
     1441 tgccgaggta gccgttgggg caatcattaa tgttagtgcg ccttgtgcaa ggatcttgtt
     1501 g
//
LOCUS       DAOPUW010000558         1497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_96249_length_1497_cov_3.952843, whole genome shotgun sequence.
ACCESSION   DAOPUW010000558 DAOPUW010000000
VERSION     DAOPUW010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96249_length_1497_cov_3.952843"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PKJ43_08660"
     CDS             complement(<1..691)
                     /locus_tag="PKJ43_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72680.1"
                     /translation="MRRIILFSVIFFIFTTNKPAKCQVIWEEKFLTPEKGYWINEEGN
                     LVSDIDQVDWQLDISGATFAAEGDFAKTTATSGGRFEVLDSDGEVVFYSPWLAISTYS
                     TVNISLKAIETGSNTGTDKKYIKAMYRTSNELIPFNAESEASGNWGEKNLVAENIKGD
                     SIQLAIIMNSSYASDKVIIDDIIVDGIDSTLLIPHHLVVYNLPSASFSGEETSFTASV
                     VNHKNQIITNKQ"
     gene            782..>1497
                     /locus_tag="PKJ43_08665"
     CDS             782..>1497
                     /locus_tag="PKJ43_08665"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNZ72681.1"
                     /translation="MKVAVVGASGAVGQEFLRVLEERNFPLTELVLFGSSRSAGNEYL
                     FKGKKLVVKELKHGDDFKDIDIALTSAGASISKEFASTITKHGAIMIDNSSAFRYDDD
                     VPLVVPEVNPEDALNRPRNIIANPNCSTIQMVVAMKPINDLSKITKVHVATYQAASGA
                     GAQGIAELEEQIKQVANGEEVKPNKFPYQLAMNIIPHIDVFLDNDYTKEEMKMNWETK
                     KIMHTDAEVSATCVRIPVARA"
BASE COUNT          438 a          285 c          321 g          453 t
ORIGIN      
        1 attgtttatt ggtgataatt tgatttttgt ggttaacaac tgatgctgtg aagctggttt
       61 cttccccgga aaaagaggca gatggaaggt tgtatactac caggtgatgg ggaatgagca
      121 aggtggaatc aatgccatca acaatgatat cgtcgatgat tactttatca cttgcatacg
      181 acgagttcat aataattgcc agttgaatag agtctccttt aatattttct gcgacaaggt
      241 ttttttcgcc ccagtttccc gatgcttcac tttcggcatt aaatggaatg agctcattgc
      301 ttgttctgta catggccttt atgtattttt tatcggtgcc ggtattgctg cctgtttcta
      361 tagccttcag cgagatattt acagttgaat aagttgaaat tgccagccat ggcgaataaa
      421 aaactacttc gccgtcggag tcgagaactt cgaatcgtcc gccagaagtc gctgttgttt
      481 tggcaaagtc gccttcagcc gcaaatgtgg caccagaaat gtcgagttgc caatcaactt
      541 gatctatatc tgaaactaaa ttgccttctt cgttgatcca atagcctttt tcgggagtta
      601 gaaacttttc ttcccaaatt acttggcatt ttgccggttt atttgttgta aatatgaaga
      661 aaatgacact aaatagaata attcgacgca taatgagcat tttttgggcg caatttttat
      721 ttttgccggg taacgtttta tgtttaaata tttaaaagaa caaatttaaa aaaaattaat
      781 tatgaaagtc gctgttgttg gtgcaagcgg cgccgtagga caagagttcc tgcgtgtgct
      841 tgaagagcgg aatttcccgc tcactgagtt ggttttattc gggtcgtccc gtagtgcagg
      901 caatgaatac cttttcaaag gtaaaaaact agtagtgaaa gagcttaaac atggagatga
      961 ttttaaagac atcgatatcg ctcttacttc agcaggcgca agtatttcca aagaatttgc
     1021 atcaacaatt actaaacacg gcgccatcat gatcgataac tcaagtgcat tccgttatga
     1081 tgacgatgtg cctttggtgg ttcccgaagt gaaccctgaa gatgcattga accgtccgcg
     1141 aaatattatt gctaatccca attgctctac tattcagatg gttgtggcca tgaagccgat
     1201 caatgacctc tctaagatta ccaaagtaca cgtagcaacc tatcaggcag ccagcggtgc
     1261 aggtgctcaa gggatagcag aacttgaaga acaaataaaa caagtggcta atggtgaaga
     1321 agttaagcca aataaatttc cttatcaatt agcaatgaac atcattcctc acattgatgt
     1381 tttcctcgat aacgattaca ccaaagagga aatgaaaatg aattgggaga caaagaaaat
     1441 tatgcatacc gatgctgaag tttcagcaac ttgcgtgcgt attcctgttg cccgtgc
//
LOCUS       DAOPUW010000559         1493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_96698_length_1493_cov_2.678720, whole genome shotgun sequence.
ACCESSION   DAOPUW010000559 DAOPUW010000000
VERSION     DAOPUW010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96698_length_1493_cov_2.678720"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PKJ43_08670"
     CDS             <1..559
                     /locus_tag="PKJ43_08670"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HNZ72682.1"
                     /translation="GLQWQWQSTPKPEWCFPTTYGFLRMNCMMIENEPLNMWDIPNLL
                     LQKFPAEQFSATTRFELHPKLEGDRAGLVVMGEDYCYLAVEYWGEKRYITFNSCTNAR
                     KEQEVQSEKQEITTPVIYMKVDVKSGGMCQFSYSEDGTKFKSIGKAFKARAGMWIGAK
                     VGIFALKKTLTNDSGYANFDWFRVE"
     gene            complement(598..1470)
                     /locus_tag="PKJ43_08675"
     CDS             complement(598..1470)
                     /locus_tag="PKJ43_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HNZ72683.1"
                     /translation="MNIMHRIILSIFALVLGGQLLAQNYTDVSLMWKDSMVQQSEISS
                     ETGNLYNKLTHHGPAIENEWLGLRLYFDYKVAVDVYNKSRKGLELKEGKWYPTAQQQS
                     EGWGADQYKVGKTVGMGGIRLWDGEKEVFLDPVKNRTARVRKEANYSSMEMLSEGIPY
                     LGDTIDVLVRVTVFSGVREAKVEAFALCDKPVRFLTGINYFPTTTIQQGDNFIATWGI
                     HPEDVAAFQVNIGGGIVFNPSDYELVKKDETQFILVSKPVKYLSTWISSSCERENELN
                     TMAKFVDYLKRIAE"
BASE COUNT          455 a          341 c          280 g          417 t
ORIGIN      
        1 aggattacaa tggcaatggc agtcaacccc aaaaccagaa tggtgtttcc ctactaccta
       61 tggattcctt cgaatgaact gcatgatgat tgaaaatgaa ccattaaata tgtgggacat
      121 tccaaacttg cttctgcaga aatttcctgc tgaacaattt tcggcaacaa cccgttttga
      181 gcttcatcct aaactcgaag gcgaccgcgc cggattggtt gtaatgggcg aagattactg
      241 ttaccttgcc gttgaatatt ggggagaaaa acgttacatc acgttcaaca gctgcaccaa
      301 tgcacgaaaa gaacaagaag ttcaatcgga gaaacaggag atcacaaccc ctgtaattta
      361 catgaaagtt gatgtaaaaa gtggcgggat gtgccagttt tcgtatagcg aagacggaac
      421 gaaattcaaa tctatcggca aagcatttaa agccagggcc ggtatgtgga ttggtgccaa
      481 agtaggcatt tttgccttaa aaaagaccct caccaatgat tcaggctatg ccaacttcga
      541 ttggttcagg gtagaataaa aagcccataa tttatgcaat aaccgctttc aaatatatta
      601 ttccgctatt cttttcaagt agtcgacaaa cttagccatt gtattgagct cgttttcacg
      661 ttcacacgaa gatgaaatcc aggtagaaag gtacttaaca ggcttcgaaa ctaaaataaa
      721 ctgagtttcg tctttcttca ccagttcata atcagatgga ttgaacacaa tgccgccgcc
      781 aatattcacc tgaaaagcag ctacatcttc aggatgaata ccccaggttg caatgaaatt
      841 atcaccttgt tgtatagttg tggttgggaa ataattaatc ccggtaagaa acctcaccgg
      901 cttatcacat aaagcaaatg cctcgacttt agcttcgcgc accccggaaa aaacagtaac
      961 ccgaaccagt acatctatcg tatcccccaa ataaggaata ccttccgaca acatttccat
     1021 agatgaatag ttggcttctt tcctgaccct tgcagtacgg tttttaacag gatcgagaaa
     1081 aacctctttc tcaccatccc acaaccgtat acctcccata ccgacagtct ttcctacttt
     1141 atattgatca gctccccagc cttcgctttg ttgttgggct gttggatacc atttcccctc
     1201 tttaagttcg aggcctttgc ggcttttatt gtacacatca acggctactt tatagtcgaa
     1261 atatagacgt aagccaagcc attcattttc aattgccgga ccatgatggg tcagtttatt
     1321 atacaaattt ccagtttccg atgatatctc tgattgttga accatggaat ctttccacat
     1381 caggcttaca tcagtatagt tttgtgcaag gagctgcccc cccagcacta acgcaaaaat
     1441 cgaaagtata attctatgca tgatattcat attttcgtga caatcaaaaa tag
//
LOCUS       DAOPUW010000560         1490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_97015_length_1490_cov_2.942857, whole genome shotgun sequence.
ACCESSION   DAOPUW010000560 DAOPUW010000000
VERSION     DAOPUW010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97015_length_1490_cov_2.942857"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PKJ43_08680"
     CDS             <1..869
                     /locus_tag="PKJ43_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ72684.1"
                     /translation="DIKAEQTSENERTNNKTTLENTYNDLVADEKEESKQKILLVEDN
                     EELLQFLCLIFGDEYEIYIASNGSEGWEQTQKHLPDLIVSDVMMPEMDGFEFCKLVKS
                     TWETSHIPVILLSALNENAKQLQGLGLGADDYITKPFDANILKHKIHNLIQSRKAYYE
                     KALQKIKEYPQEQLLANPMNDKFLKQMVDVVKENMANTQFGKVEFASALCVSPSLLYK
                     KTKAIAGLSPVDFIKSVRLDHSIQLLQNKEYTIAEISELCGFSNQSYFSAVFKKKFGK
                     TPSEYYDTTNDR"
     gene            complement(1005..>1490)
                     /locus_tag="PKJ43_08685"
     CDS             complement(1005..>1490)
                     /locus_tag="PKJ43_08685"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNZ72685.1"
                     /translation="SFRIVDEMPIVNKYSLDESSTWELGSYIILYRAADIHLLLAEAL
                     NRSGSSPVALNIMNRGHKTFPNWSENAGIRGRAYLQPREVPEGVDSVTFIEDLIIEER
                     SMELAFEGKRWFDLMRLARRRGNEYLADRVAAKFTDPSAAQRVREKLMNEQNWYIPFV
                     K"
BASE COUNT          509 a          315 c          250 g          416 t
ORIGIN      
        1 ccgacataaa agctgagcaa acttctgaaa atgaaaggac aaacaataaa actactcttg
       61 aaaacacata taatgattta gttgcagacg aaaaagaaga aagcaaacaa aaaattcttt
      121 tagtagaaga caacgaagaa cttcttcagt ttctttgcct gatttttgga gatgaatatg
      181 aaatatatat agcatccaat ggctccgaag gttgggaaca gacacaaaaa cacctgcccg
      241 atttaattgt ttctgacgtg atgatgcccg aaatggacgg gtttgaattt tgtaaattgg
      301 taaaatctac ctgggaaaca tcccatattc ctgtaatttt actttctgcg cttaacgaaa
      361 atgccaaaca attacaagga ttaggccttg gagccgacga ctacataacc aaaccgttcg
      421 acgccaatat tttaaaacat aaaatacaca atctcataca aagccgaaaa gcctattatg
      481 aaaaagctct ccaaaaaata aaagagtacc cccaagagca gctactggca aacccaatga
      541 atgacaagtt tttaaagcaa atggtcgatg tagtaaaaga gaatatggcc aacacacaat
      601 ttggaaaggt tgagtttgct tcagccctgt gtgtaagccc atcgctcctc tataaaaaaa
      661 ctaaagcaat tgccggatta tcgcctgtcg atttcattaa atcggttagg ctcgatcatt
      721 caattcaatt acttcagaac aaagaatata caattgcaga aatcagcgag ctgtgtggat
      781 tctcaaatca atcgtacttt agtgctgtat tcaaaaagaa atttgggaaa acgccttccg
      841 agtactatga cactacaaat gaccgataaa atcactttcg ttgtaattgt cttaaaatga
      901 caaaatagaa atagggttaa gaacaaatta ttctcaaccc taattttatt ttcgatcaaa
      961 agctcacaac cttatcagct tttgtttccc cattcaacag gtgcttattt cacaaaagga
     1021 atataccaat tctgctcgtt cataagtttt tccctaacac gctgtgcggc tgaaggatca
     1081 gtaaacttag cagcaacacg atcggctaaa tattcatttc ctcttcttcg ggcaagtctc
     1141 ataaggtcga accacctttt cccctcaaaa gcgagttcca tagatcgctc ttcaatgatt
     1201 aaatcttcga tgaaggttac agaatcaaca ccttcgggca cttctcttgg ttgaaggtaa
     1261 gcacgccccc gaatgcctgc attttcgctc caatttggaa acgttttatg tccccggttc
     1321 attatattta aagctaccgg tgaacttcca ctcctgttca atgcttcggc gagtagcaaa
     1381 tgaatatcgg ctgcccgata aaggattata tatgacccca attcccatgt actgctttca
     1441 tctaaactat atttattaac tattggcatt tcatcaacaa ttctaaaaga
//
LOCUS       DAOPUW010000561         1489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_97120_length_1489_cov_2.833333, whole genome shotgun sequence.
ACCESSION   DAOPUW010000561 DAOPUW010000000
VERSION     DAOPUW010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97120_length_1489_cov_2.833333"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..407)
                     /locus_tag="PKJ43_08690"
     CDS             complement(15..407)
                     /locus_tag="PKJ43_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72686.1"
                     /translation="MKTIVLLIIGIMLSVCVSAQHRAVYGNVRLSDDLKLSNIVVKAK
                     KAGTTTITDSLGNFVIVCNKKDVLQFEAKTFEKVSKRVRPSTDTVLVQMRFASGPESV
                     QLAVGYGYISQDKATHAQSMLDNQRNEF"
     gene            complement(688..>1489)
                     /locus_tag="PKJ43_08695"
     CDS             complement(688..>1489)
                     /locus_tag="PKJ43_08695"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNZ72687.1"
                     /translation="IVARQFIFRMREFEQMEMQFFVKPGTELDWFEKWKTTRMSWHQS
                     LGFGAENYRFHVHEKLAHYANAAVDVEYKFPFGFKEVEGIHSRTDFDLSQHQQYSGKK
                     IQYFDTEKNESYVPFVVETSIGVDRMFLQVLSASYKEEEVSDANGQKDTRVVLSLPPQ
                     LAPVKMAVMPLVKKDGLPEKAREIIDDLKFDFNCMYDEKDSIGKRYRRMDAIGTPFCV
                     TVDHQTLEDNTVTIRYRDTMEQDRVEISKLRAIIAEAVSMKSLFAKLK"
BASE COUNT          398 a          334 c          295 g          462 t
ORIGIN      
        1 tgcccaatcg atgctcaaaa ttcattcctc tgattatcga gcatcgattg ggcatgcgtg
       61 gccttatctt gtgaaatgta tccataaccc acggcaagct gaacgctctc gggtccactg
      121 gcaaatcgca tctgaactag cacggtatcg gttgaaggac gtacccgctt tgatactttt
      181 tcgaaggttt tagcttcgaa ctgaaggaca tctttcttat tgcaaacaat aacaaagttt
      241 cccaacgagt cggtaattgt tgtcgtccct gcttttttcg ctttaacaac aatgtttgaa
      301 agcttcagat cgtcggaaag acgaacattt ccgtaaactg cgcggtgctg tgcactaaca
      361 caaaccgaca acatgattcc gataattagt aatactatgg ttttcatgac tttatttttt
      421 aaaaagattc aacattctgt atgaacttgg cctctctgga tcctatttag ccgcattcac
      481 aattatgaca cgtagaaaga aaattgcccc taccctgttg ttgccctttt tattttttca
      541 tgattttttc caaccattct tttgaatgat atgacaatct tatacgtgaa atgtttgcaa
      601 aagctacaag ataaaacaaa aaaaggctgc acatggcagc ctcctttata tcaatatatt
      661 agaatagaat ctaaagaatc gggtgttcta tttaagtttt gcaaataagc ttttcatgct
      721 aactgcctca gcaatgattg cccgtagttt cgaaatctca acacggtctt gttccatcgt
      781 atcgcggtaa cgaatggtca cggtattgtc ttccagggtt tgatggtcga cagttacgca
      841 gaatggtgtg ccaatggcat ccatacggcg gtaacgtttc ccaatagagt ccttttcgtc
      901 gtacatacag ttaaagtcga acttcaggtc gtcgattatt tcacgggctt tttctggtag
      961 cccatctttc ttcaccaatg gcatcacggc cattttaaca ggagctaact gtggcggcaa
     1021 actaagtact acgcgggtgt ctttttgacc gttggcatcg cttacttctt cttctttgta
     1081 tgaagccgaa agaacttgca ggaacatgcg gtcgacccca attgaagttt caaccacaaa
     1141 tggcacatac gattcgtttt tttcggtgtc gaaatattga attttcttgc ccgaatattg
     1201 ctgatgttgg ctcagatcga aatcggtacg ggagtggatc ccttccactt ctttgaaccc
     1261 aaacgggaat ttatattcca catcaacagc agcattggca tagtgtgcca gtttctcgtg
     1321 tacatggaaa cggtaatttt cagcaccaaa accaagcgat tgatgccagc tcatacgggt
     1381 tgttttccat ttttcgaacc agtcgagttc tgtgcccggt tttacaaaaa actgcatttc
     1441 catctgctcg aattccctca tgcggaaaat gaactgacgg gctacaatt
//
LOCUS       DAOPUW010000562         1489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_97175_length_1489_cov_1.486053, whole genome shotgun sequence.
ACCESSION   DAOPUW010000562 DAOPUW010000000
VERSION     DAOPUW010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97175_length_1489_cov_1.486053"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..451)
                     /gene="atpC"
                     /locus_tag="PKJ43_08700"
     CDS             complement(197..451)
                     /gene="atpC"
                     /locus_tag="PKJ43_08700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNZ72688.1"
                     /translation="MNLKIVLPNKLIYDGEISSVTVPGKGGVFTILSHHAPLIAILGK
                     GNVTFRFKNGVNNVFINGGLIELNNDKVVICAEQIPENEK"
     gene            complement(448..>1489)
                     /gene="atpD"
                     /locus_tag="PKJ43_08705"
     CDS             complement(448..>1489)
                     /gene="atpD"
                     /locus_tag="PKJ43_08705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNZ72689.1"
                     /translation="ELINNIAKGHKGFSVFAGVGERTREGNDLLREMIEAGVIRYGEE
                     FNKAMEEGKWDLSKVDYEQLKKSQATLVFGQMNEPPGARFSVALSGLTVAESFRDSEE
                     SENRDILLFIDNIFRFTQAGSEVSALLGRLPSAVGYQPTLATEMGLLQERITSTKHGS
                     ITSVQAVYVPADDLTDPAPATTFSHLDATTVLSRQIASLGIFPAVDPLDSTSRILDPL
                     IVGQEHYETAQRVKQILQRNKELQDIIAILGIEELSEEDRLTVQRARKVQRFLSQPFF
                     MAEHFTGVPGVMVPIEETIHGFKLILDGMLDDLPEMAFLNVGTIKDVLNKGHALLREA
                     HNLNKTFKIEDQ"
BASE COUNT          474 a          315 c          274 g          426 t
ORIGIN      
        1 aaattttgat tattattaac aatcagggaa cggataccct gcaatgtggg ttaaattcaa
       61 tccagaaaaa taaaacaata aaggcaacaa aaaataattt tcgatagtcg tggaagaaaa
      121 ttttttcaag agcaaccaga aaaatagccc taacaacaat gttagtaaag aaaaaaaaac
      181 taatgaaaaa agaagattat ttttcatttt ctggaatttg ttcagcacaa ataacgacct
      241 tatcgttatt caattcgatt agtccgccat taataaaaac attgttaaca ccgtttttga
      301 aacgaaaagt aacatttcct ttccccaaaa tggcaatcaa aggagcgtga tgagaaagta
      361 tggtaaatac ccccccctta cccggtactg ttacagatga aatttcacca tcgtaaatca
      421 atttattggg caaaacaatt ttcagattca ttgatcctca attttaaatg ttttattcaa
      481 attgtgagct tctcttagca atgcatgccc cttatttaag acatccttaa tggtaccaac
      541 attaagaaaa gccatttcgg gaaggtcatc gagcataccg tccaatataa gtttaaagcc
      601 gtgaatggtt tcttctatag gtaccattac tcccggaact cctgtaaaat gttctgccat
      661 aaagaaaggc tgcgaaagaa aacgttgtac ttttcgagct ctttgcactg ttaaacgatc
      721 ttcttcagag agttcttcta tacccagaat ggcgataata tcttgtaatt ccttgtttcg
      781 ttgaagaatt tgcttcaccc gctgagctgt ttcataatgt tcttggccga caataagcgg
      841 atcgagaatt cgcgaagtgg aatccagtgg atcgacagca ggaaaaattc ccaatgaagc
      901 aatttgccga ctcaataccg tagtagcatc caaatgcgaa aaagtagttg ccggagcagg
      961 gtcggtcaag tcatcagccg gcacgtaaac agcctgaacg gaagtaatgg atccatgctt
     1021 tgtggaagta atacgttcct gtaaaagtcc catttccgta gccaacgttg gctgatagcc
     1081 tacagccgat ggtaaccgtc ccaagagagc cgaaacttca gaaccggctt gcgtgaaacg
     1141 gaaaatatta tcgataaaca ataaaatatc gcgattttca ctttcttcac tgtcacggaa
     1201 cgactctgcc acagtaagcc cggataatgc aactgaaaaa cgtgctcccg gtggttcatt
     1261 catctgtcca aagaccaaag ttgcctgaga ttttttcagt tgttcgtagt caacttttga
     1321 caaatcccat ttgccttctt ccattgcctt gttaaattct tctccgtagc gtattacacc
     1381 ggcttcaatc atttcgcgaa gcaaatcgtt tccttcacgg gttcgttcgc caactccggc
     1441 aaatacggaa aatcctttat ggcctttggc tatattgttg ataagttcc
//
LOCUS       DAOPUW010000563         1484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_97682_length_1484_cov_2.275017, whole genome shotgun sequence.
ACCESSION   DAOPUW010000563 DAOPUW010000000
VERSION     DAOPUW010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97682_length_1484_cov_2.275017"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PKJ43_08710"
     CDS             <1..205
                     /locus_tag="PKJ43_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HNZ72690.1"
                     /translation="IPWLDFTGISHARSFSHPDSCPKISFGKVTEKEGIKTMPVSVHV
                     HHALVDGYHVGLFIDKFQDLLND"
     gene            complement(329..1363)
                     /locus_tag="PKJ43_08715"
     CDS             complement(329..1363)
                     /locus_tag="PKJ43_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ72691.1"
                     /translation="MTDQQWNRLTALLKGEGSGTLPAGFIIDCPWLPKWYGISILDYF
                     SNDDLWLKANLKAIETFPDVMFLPGFWSEYGMCTEPSAFGAKSVFWKNEFPFAEKVIH
                     EPEDIDSLQVPEPATDGLLPFMLNRLVMARPAIEAAGHKIRFSVSRGPLNVASFLMGT
                     TELMTTMMMDPDRVHKLLRVITDFLKKWHQLQRETIPTIDGMLMLDDIVGFVGEDEFL
                     EFGYPYLKELYSADVAVKFFHNDADCSMSVKYYPEIGINLYNPGLDLSISEIQDKTDH
                     KLVVLGSLPPRDVLAACSPDEVYAQTKAMIEGVTDRKMFLPSCGGGMPPNVSTENINA
                     FVKAVKEYTF"
BASE COUNT          435 a          327 c          309 g          413 t
ORIGIN      
        1 aataccatgg ctcgacttta cgggcatatc tcatgcacga agtttttcgc atcccgacag
       61 ttgcccaaaa atttcatttg gaaaagtaac tgaaaaagaa ggaattaaaa caatgccagt
      121 atctgttcat gtgcatcacg cgctcgtgga tggctatcat gtcggattat ttattgataa
      181 atttcaggat ctgctaaacg actaaaaaac aaccggatat aacaagttgt aatcctgttt
      241 taaatgggca atttttatat cgggcaacaa aaaactccgg aaacattatg taaccggagt
      301 gtgaagatta ttgctaaaac aatcgttatc aaaatgtata ttctttcaca gctttaacaa
      361 aagcattaat attttcggta ctcacattgg gtggcatacc tcctccgcac gaaggtagga
      421 acattttccg gtcggtaacc ccttcgatca tggccttggt ttgcgcataa acctcatcgg
      481 gcgagcaggc ggcaagcaca tcgcgcggtg gcaaactacc cagcactacc agcttgtggt
      541 cggttttatc ctgaatttct gaaatagaaa ggtcgaggcc tgggttgtac aaatttatgc
      601 caatttcagg atagtacttc accgacatag agcaatcggc atcgttgtgg aaaaatttca
      661 cagccacatc ggcactatac agttctttca gataaggata accaaattcg agaaattcat
      721 cttcgccaac aaaaccaaca atgtcgtcga gcataagcat cccgtcgata gttggaatgg
      781 tttcccgttg cagttggtgc catttcttca gaaaatcggt aatgacccgc aataatttgt
      841 gaacccggtc gggatccatc atcattgtag tcatcagctc ggtggtcccc atcaggaacg
      901 acgccacatt tagcgggccg cgcgacactg aaaaacggat tttatgacca gcagcttcta
      961 tggcaggccg ggccataaca agcctgttga gcataaacgg taaaagcccg tcggttgcag
     1021 gctccggtac ttgcagtgaa tcaatgtctt ctggttcatg aatgactttt tcggcaaaag
     1081 gaaattcatt tttccagaat acacttttgg ccccaaaggc cgatggctcg gtacacatgc
     1141 cgtattcgct ccaaaagcca ggtaaaaaca ttacgtcggg aaatgtttca atggctttta
     1201 aatttgcttt caaccataag tcatcgttcg aaaaatagtc gagaatcgaa attccgtacc
     1261 atttgggaag ccaagggcaa tcgatgataa aacctgcggg caaggtaccg ctcccttcac
     1321 ccttaagtaa ggctgtaagc ctgttccact gctgatctgt catattttta tatttttagt
     1381 tttacaaaca atccggtatt cacattttat tttgatgtat ccttatcgga acttttacat
     1441 gtggcgacta atttataaac ttcagcgttg gaatgaaaga tatt
//
LOCUS       DAOPUW010000564         1481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_97977_length_1481_cov_3.126928, whole genome shotgun sequence.
ACCESSION   DAOPUW010000564 DAOPUW010000000
VERSION     DAOPUW010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97977_length_1481_cov_3.126928"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /locus_tag="PKJ43_08720"
     CDS             <1..131
                     /locus_tag="PKJ43_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNZ72692.1"
                     /translation="YTYDPELGEPENGIPPGTPFDALPEDYICPVCRASKEYFREI"
     gene            complement(186..1328)
                     /locus_tag="PKJ43_08725"
     CDS             complement(186..1328)
                     /locus_tag="PKJ43_08725"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010423646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HNZ72693.1"
                     /translation="MKKALYITAAIAIIFLAACNSKSTPFTKTVEAISATNFQKDIDG
                     KATSLYTLTNQNGMGIKVTNFGARIVALCVPDKSGKPVDVVFGYSTIDEYLNQPEKFY
                     GAAIGRYGNRIAKATFSIDSVSYSLAANNGINHLHGGPKGYYDVVWNAEQSSPSKVIF
                     TYNSADGEEGYPGNLSISMTYELTEDNGLKIEYAATTDKKTICNLTNHSYFNLSGEGE
                     ATINDHLLMIKADKYTPVDSTLIPTGELAPVEGTPMDFTKPTAIGSRVGESFEQLSFG
                     GGYDHNWVISKETSGVELIASLESPVTGIKMEVYTDQPGIQFYGGNFLTDNDKGKSGK
                     PYQYRSGLCLETQHFPDSPNQPEFPTTLLEPGQNYSHTCIYKFSAK"
BASE COUNT          373 a          291 c          326 g          491 t
ORIGIN      
        1 gctacactta cgacccagaa ttgggtgagc ctgaaaatgg tatacctccg ggaactccgt
       61 tcgatgcgct gcccgaagat tacatttgtc cggtctgccg tgcgtcgaaa gaatatttcc
      121 gtgaaatatg aaaaaagggg tagccaaata tggttacccc tttttttgat ctgttttgcc
      181 aacttttatt tggctgagaa cttatagata caagtgtgcg aataattctg gcctggctcg
      241 agtagagtag ttggaaactc tggctgatta ggtgaatcgg ggaaatgttg tgtttcaaga
      301 cacaacccgc tacggtattg gtaaggtttt cctgattttc ctttgtcgtt atcggttaag
      361 aagttgccac cgtagaattg aatgccgggc tggtctgtat aaacttccat tttgatacca
      421 gtaacaggag attcaagtga ggcaattaat tcaacgcctg aagtttcttt gctgataacc
      481 cagttgtggt catagccgcc gccaaacgat agttgttcaa agctttcgcc aactctcgaa
      541 ccaatagctg ttggttttgt aaaatccatt ggagtacctt caactggtgc aagctctcct
      601 gtagggatca aggttgaatc aaccggggta tatttgtctg ctttgatcat caaaagatgg
      661 tcgttgatcg tagcttcgcc ttcgcccgaa aggttgaaat acgagtggtt ggtgaggttg
      721 cagattgtct ttttatcagt agtggctgca tattcaattt ttaacccatt gtcttcggtt
      781 aattcgtatg tcattgaaat acttaagttt ccggggtatc cctcttcgcc atcagccgaa
      841 ttgtacgtga aaatcacttt tgacggactg ctttgttctg cattccaaac cacatcgtaa
      901 tatccttttg gcccgccatg tagatggtta atcccgttgt ttgcagctaa actatagctt
      961 acactatcga tgctgaaagt tgccttagca atgcggtttc cataacgacc aattgcagca
     1021 ccgtagaatt tttctggttg attaaggtat tcatctatag tgctatatcc aaatacgaca
     1081 tctacaggtt ttcctgattt atcgggcaca caaagggcca caatacgtgc tccgaaattg
     1141 gtcactttaa ttcccatccc gttttgattt gttaaggtat aaagcgaagt ggctttgccg
     1201 tcaatatctt tttggaaatt agttgctgaa attgcttcaa ctgtttttgt aaacggagtc
     1261 gatttgctgt tgcaagcggc caagaaaata atggcaatcg ctgcggtaat atataatgct
     1321 tttttcattg ttgtaaattt ttttatttgt caaattattg aagttgaaca agtaccactg
     1381 agtgggcagg gagttcaata ctaagttttc cgtttttggg ttttgccaca gtaaagtctt
     1441 gcatgtttac tttttctgct tgcccatagt cgttatagtc a
//
LOCUS       DAOPUW010000565         1480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_98081_length_1480_cov_2.815439, whole genome shotgun sequence.
ACCESSION   DAOPUW010000565 DAOPUW010000000
VERSION     DAOPUW010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98081_length_1480_cov_2.815439"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PKJ43_08730"
     CDS             <1..205
                     /locus_tag="PKJ43_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNZ72694.1"
                     /translation="GKNSSNGKALFEVCRKTNPSTHFVVSPGDIELDWFSGVQRVGIC
                     GATSTPQWLMEQVRNHVRTILNL"
     gene            287..1282
                     /gene="pfkA"
                     /locus_tag="PKJ43_08735"
     CDS             287..1282
                     /gene="pfkA"
                     /locus_tag="PKJ43_08735"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNZ72695.1"
                     /translation="MTEKRKGIKRIGVFTSGGDAPGMNAAVRAVTRSAIAHKLEVVGI
                     RRGYQGMIEGDFVKLRSSDVSGIMQLGGTFLQTARSTEFRSDEGMAKAYQQLKSQEID
                     AVVVIGGDGSFRGALDFSQQYDIPFVGIPGTIDNDMYGTDYTIGFDTALNTVVEAVDK
                     IKDTASSHGRIFFVEVMGREAGQLALKAGVACGAEVILIPEIQSDEIELEKFITKGYK
                     SKKRSGIIMVAEGDKAGGAFKLAEKVQKEHPDLDVRVSILGHMQRGGNPSAIDRVKAT
                     QLGVAAVEALLDDQKSIMVGLKDDKIVHVPMVKVVKMNRDIDNDLLNIHRTINTL"
BASE COUNT          438 a          260 c          368 g          414 t
ORIGIN      
        1 tgggaaaaac agttcgaatg gcaaagccct tttcgaagtt tgccgtaaaa caaacccgtc
       61 aacccatttt gttgtttctc caggtgatat agaattggat tggttttctg gggttcaacg
      121 ggttgggatt tgtggggcaa catcaacccc tcagtggtta atggagcagg ttcgaaacca
      181 tgttagaact atcttaaatc tctagcctgc ttatccaaaa aagaatttgc tatcgacatt
      241 ggtgctaatt cttgttattt ttgtcaaata ttcaaaagtc aacactatga cagaaaaacg
      301 taaaggtata aaacgcattg gggtatttac ctcgggaggc gatgcccctg gaatgaacgc
      361 agctgtaagg gccgtaacac gctcggcaat tgcccacaag ctcgaagtgg ttggtattcg
      421 cagaggatat cagggcatga ttgaaggcga tttcgttaaa ttacgatcga gcgatgttag
      481 cgggataatg caattgggtg gtactttttt gcaaacggcc cgaagtacag agttccggtc
      541 agatgaaggc atggctaaag cttaccagca attgaaaagt caggaaattg atgccgtggt
      601 agttatcgga ggcgatggaa gtttccgcgg ggcgctcgat ttctcgcagc agtatgatat
      661 tccatttgta gggattcctg gaactattga taatgacatg tacggaactg actacaccat
      721 tggttttgat actgcattaa atactgttgt tgaggctgtt gataaaataa aagatacagc
      781 gagttcgcat ggccggatat tttttgttga agtaatggga cgtgaagccg ggcagcttgc
      841 tttgaaagcc ggagttgcct gtggagctga agttatactc atccccgaaa ttcaatctga
      901 cgaaatagaa ctggagaaat ttataactaa aggatataaa agtaaaaagc gctcagggat
      961 cattatggtt gcagaaggag ataaagctgg gggagcgttt aaacttgccg aaaaagttca
     1021 aaaagagcat cccgaccttg atgtgcgtgt atcaatatta ggtcatatgc aacgcggggg
     1081 caacccatcg gccatcgaca gggttaaagc cactcagttg ggtgtagctg ccgttgaagc
     1141 tctgctcgac gatcagaaaa gcattatggt tggacttaaa gacgacaaaa ttgtgcatgt
     1201 gcctatggtg aaggttgtga agatgaatcg cgacattgat aacgatttgc ttaacattca
     1261 tcgtacaata aatacacttt aaacgatatt ttactattgt tcagaaaagc agcacagtta
     1321 ccgaatcttc ggaggctgtg ctgctttttt tatgggaagg gttgatgaac acaattgatc
     1381 aacgctaatg gttttgatta attagggcac aaaaaaatcc cggaacaaat aattgccggg
     1441 atttctgtgt ttatcttatt taattataag cttttgatgt
//
LOCUS       DAOPUW010000566         1472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_98904_length_1472_cov_3.427664, whole genome shotgun sequence.
ACCESSION   DAOPUW010000566 DAOPUW010000000
VERSION     DAOPUW010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98904_length_1472_cov_3.427664"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PKJ43_08740"
     CDS             complement(<1..628)
                     /locus_tag="PKJ43_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72696.1"
                     /translation="MMMKQNLSMKKYLCCIQKLSQTGALLLAIFAIFMVLGCSGNKAK
                     KAWNEASNILASISEPEFKEQTYIITDFGASPGTNIMNTDAINHAINFCSAEGGGIVL
                     VPDGVFFTGPLELKSNVRLHLSDNATLSFSTNPKDYLPVVYTRWEGIDCYNYRPLIYA
                     NQARNIAITGKGLLKGNASTDNWWKWKGRTEYGWVEGEPSQLLPQGRPL"
     gene            complement(749..>1472)
                     /locus_tag="PKJ43_08745"
     CDS             complement(749..>1472)
                     /locus_tag="PKJ43_08745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ72697.1"
                     /translation="KKVEELAESAELIARSERESAWREMARQIAHEIKNPLTPIKLNI
                     QFLQRTNPSSVENYQEIVDRVSHTIIEQIDNLSNIATEFSNFAKIPKAKNERFNLIGR
                     LEEIISLFDYTGECSIDRKFKGAEGIEIFADKEQFSRAVINLVRNAIQSIPENRAGKI
                     TVSAMLEKGAVVVSVADNGKGISPEMEENIFVPNFTTKTSGTGLGLAITKGIVENFRG
                     EIWFSSELNTGTTFYIRIPIAK"
BASE COUNT          429 a          323 c          264 g          456 t
ORIGIN      
        1 gcaagggccg tccctgtggc aacaattgac taggttcacc ttctacccag ccatattctg
       61 tacgtccttt ccatttccac caattatcag tactcgcatt gcccttcaac aaacccttcc
      121 ctgttatggc aatattacgg gcttggttgg cataaatcaa cggacgataa ttataacaat
      181 cgatgccttc ccaacgggta taaaccacgg gtaaataatc tttcggattt gttgagaatg
      241 aaagtgttgc attatcactc aaatgcaaac gcacattcga ttttaattcc aatggtccag
      301 taaaaaatac cccatcaggg acaagcacta tacccccacc ttcagcactg caaaaattaa
      361 ttgcatggtt gatagcatcg gtgttcataa tatttgtacc cggagatgct ccaaaatcgg
      421 ttattatata ggtttgttct ttaaactcag gctcagaaat ggaggctaaa atatttgatg
      481 cttcattcca tgcttttttt gctttgttac ctgaacaccc aagcaccatg aatatggcaa
      541 aaattgctaa aagtaaagct ccggtttgac tcagtttttg tatgcaacac aggtattttt
      601 tcatcgacaa gttttgcttc atcatcataa attttcacac acaatgttac aaaaagacgg
      661 agcaagcaaa tagttacaaa ttgccgaaat atacggtgca ttctgacttt taacaatgaa
      721 aacgataaag acagaaccaa gctgtcagtt atttagctat tggaatccga atataaaacg
      781 ttgtaccagt atttaattct gaagaaaacc atatttctcc cctgaaattc tcaacaattc
      841 ctttggttat ggcaagacca agaccggtac cactggtttt tgtagtaaaa ttgggaacaa
      901 aaatgttctc ctccatctcg ggtgaaatcc ctttaccatt gtcggcgaca cttacaacaa
      961 ctgctccttt ttcaagcata gcagaaacag taattttacc ggctctgttt tcaggaatcg
     1021 actgaatcgc gtttcgaacc aaattaatta ctgcacgtga gaattgctcc ttatctgcaa
     1081 aaatctcaat gccttcggct cctttaaact ttctatcgat ggaacattcc ccggtataat
     1141 caaagagcga gattatttct tcaagccggc caataagatt gaaacgttcg ttttttgctt
     1201 taggaatttt cgcaaagttt gagaactcgg tggcaatatt tgaaaggtta tcaatttgct
     1261 cgataatcgt gtgactcacc ctatctacaa tctcctgata attttctacc gatgacggat
     1321 tagtgcgttg caggaactgt atattaagtt ttattggtgt gagcgggttt ttaatttcat
     1381 gggcaatttg acgggccatt tcgcgccatg cagattctcg ttccgagcgg gctatcaact
     1441 cggcactttc ggctaactct tccacctttt tg
//
LOCUS       DAOPUW010000567         1469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_99271_length_1469_cov_2.147100, whole genome shotgun sequence.
ACCESSION   DAOPUW010000567 DAOPUW010000000
VERSION     DAOPUW010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99271_length_1469_cov_2.147100"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..408
                     /locus_tag="PKJ43_08750"
     CDS             199..408
                     /locus_tag="PKJ43_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNZ72698.1"
                     /translation="MKPLSEFVKERRKEVNLTQEEFAERTGVALTVIRKIEQGKTNLN
                     LDKVNLVLSMFGHELAPVNQKEIER"
     gene            405..740
                     /locus_tag="PKJ43_08755"
     CDS             405..740
                     /locus_tag="PKJ43_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HNZ72699.1"
                     /translation="MRSGWVYYKEWLAGEISETNEGEFIFRYDENYVREHPNAPISLT
                     MPVKENPYHEKRLFPFFEGLIPEGWLLDIATKNWKIKANDRMGLLLACCQNCIGAVSV
                     FPKQNEDGK"
     gene            730..>1469
                     /locus_tag="PKJ43_08760"
     CDS             730..>1469
                     /locus_tag="PKJ43_08760"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HNZ72700.1"
                     /translation="MENKCLFCYQTLDENADFHSKCLASFFGSPVSPKLPYSINQMKE
                     LAKEVIERSVTVPGVQAKLSMSLIGDTSNDKNARLTVVGALGGQYILKPPTAHFPEMP
                     SNEHLTMRIAEAFGIRVVPSTLIRLETGELAYLTKRIDRNEKTGAKIHMLDMFQLTEA
                     YDKYKSSMEKVGKALHNYSSNTLLDKLFLFELTLFCFLTGNNDMHLKNFSMIESASGW
                     ILSPAYDLQNVAIILPDDNEELALTLGG"
BASE COUNT          483 a          298 c          298 g          390 t
ORIGIN      
        1 tgttcttggc tatatgcttt cacatacaat tttcacactt ttgattcaat tatatctttt
       61 tgcatatatt tttgtatatt tgagcgaaat tatatcaaaa tacatataat gaaacgagcc
      121 aagtaagtat ctcacaaaaa aggatgatta aatgtgtggc gagttccatg agataccaaa
      181 aattttaaat ttaatcgaat gaaaccattg tccgaatttg taaaagagcg aagaaaagaa
      241 gtgaacctca cccaagagga gtttgccgaa cgcacaggtg ttgccctaac ggtaatccgc
      301 aaaattgaac aaggaaaaac gaacctgaac ttggacaagg ttaacttggt actgagcatg
      361 ttcggccatg aactggctcc ggttaaccaa aaagaaattg aacgatgaga agcggttggg
      421 tgtattacaa agagtggttg gctggggaaa tttccgaaac caacgagggc gaatttattt
      481 ttcgctacga tgaaaattac gttcgtgaac acccgaatgc tcccatatcg ttaaccatgc
      541 ctgtaaaaga aaacccctat catgaaaaaa ggctattccc attttttgaa ggattaattc
      601 cagaaggatg gttgttggat atagccacca aaaattggaa aatcaaagcc aacgatcgaa
      661 tggggctgct actggcttgt tgccaaaatt gcatcggtgc tgtaagtgta ttcccaaaac
      721 aaaacgaaga tggaaaataa atgccttttc tgttaccaaa cacttgatga aaacgctgat
      781 tttcacagca aatgtttggc ctcatttttt ggttcaccag tttccccaaa gttgccttat
      841 tcaatcaatc aaatgaaaga actggcaaaa gaagtgattg agcgaagtgt aaccgttcct
      901 ggtgtacagg caaaattatc catgtcgtta attggggata cctcaaatga taaaaatgcc
      961 cgcctaacgg ttgttggagc ccttggcgga caatatattt tgaaaccacc aacagcgcat
     1021 tttcctgaaa tgccctccaa tgaacacctt accatgcgaa ttgccgaagc atttggaatc
     1081 cgggtagtcc catcgacgct aatccgattg gaaactggag agttggccta cctgaccaaa
     1141 agaatagacc gaaacgaaaa aacgggcgca aaaatccaca tgctggatat gtttcaactt
     1201 accgaagctt acgacaagta caaaagctcg atggaaaaag tgggcaaggc tcttcacaac
     1261 tattcaagca ataccttact cgacaaactc tttctgttcg aattgactct attctgtttt
     1321 cttacaggaa acaacgacat gcacctgaaa aacttttcta tgatcgaaag tgcttcgggc
     1381 tggatccttt cgccagcata cgatttgcaa aatgttgcca tcattttgcc cgatgacaat
     1441 gaagaattag ccctcacttt gggcggaag
//
LOCUS       DAOPUW010000568         1468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_99354_length_1468_cov_2.621373, whole genome shotgun sequence.
ACCESSION   DAOPUW010000568 DAOPUW010000000
VERSION     DAOPUW010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99354_length_1468_cov_2.621373"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..936
                     /locus_tag="PKJ43_08765"
     CDS             94..936
                     /locus_tag="PKJ43_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72701.1"
                     /translation="MKKRILTLLTIIGLSSSLLASTPSDSIPKYVYLAFGGGLNRTSV
                     NETLNTSLSYTGFSPGFSVEYSMATSKMYVELRNVFSIGSLSPYKAGSNFNNTMQNIY
                     ENINLNAQWLLYKKPESKFYTYAGPMVSGKVQVRGKNSNISNSAYTYDISASFGLAAK
                     VDKYVKIDHIFSWPINEQNFKLGASISYPLVSNVWSQPYSGITKNIAQEGGMLLDLSN
                     SYFGYFLNYTNIEVNLAVAYLLKNKNAIELSYMHDYNSTQPKHNPSKTLNQMLSLKLY
                     FNFK"
     gene            945..>1468
                     /locus_tag="PKJ43_08770"
     CDS             945..>1468
                     /locus_tag="PKJ43_08770"
                     /inference="COORDINATES: protein motif:HMM:NF026035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72702.1"
                     /translation="MKKLIQPIIFIAAMFSLVSCASSILEEPSTDKKEIFEYAWKTID
                     ERYSFFEMKNIDWDAVHARFEPGITNSMSDEQFFDTLGVMLDILKDGHTGVTSPFNSH
                     FYIGFFTRGIENYDHRLIVDNYYHSWGNRKGSFYHTALAENQVGYIAYSDFSNSFTDE
                     DLDYLLTKYKDTKG"
BASE COUNT          478 a          325 c          255 g          410 t
ORIGIN      
        1 ccaggcagcc ttttttttgt tataacaatt ttttatatca ccgatcaatg caaccattta
       61 ctcatacttt tgtcattacg acaaaaaaca attatgaaga aacgaatttt aaccttacta
      121 actattattg gacttagttc ttcgctatta gcctcaaccc cgtcggattc aattccaaag
      181 tatgtttatc tggcatttgg gggtggacta aacagaacct cggtaaacga aacattaaac
      241 acaagcttgt cctacaccgg attttccccc gggttttcgg ttgagtattc tatggctacg
      301 tcgaaaatgt atgttgaatt aagaaatgta ttttcaatcg gcagcctttc gccatataag
      361 gccggaagta actttaacaa cacgatgcaa aacatttatg agaacataaa tctaaatgcc
      421 cagtggctat tatacaagaa acccgaaagt aaattttaca cctatgccgg gcctatggtc
      481 agcggtaaag tacaggtaag gggcaagaat tctaatattt cgaacagtgc ctacacttac
      541 gatatctcag catcgtttgg gcttgctgca aaagttgata aatatgtaaa aatcgaccac
      601 atattttcgt ggccaattaa cgagcaaaac ttcaaactgg gagccagtat ctcctaccct
      661 cttgtttcaa atgtctggtc gcagccatac tccggaatca ctaaaaatat tgcccaagaa
      721 ggcggcatgc tactcgacct ttcaaatagt tactttggat atttcctaaa ctacacgaac
      781 attgaagtta atttagccgt ggcatatctg cttaaaaaca aaaatgccat tgaattatcc
      841 tatatgcatg attacaattc gacacaacca aagcacaacc cctcaaaaac gctcaaccag
      901 atgcttagcc ttaaactata cttcaacttc aaataaataa aatcatgaag aaattaattc
      961 aacctatcat attcattgca gcaatgtttt cgctcgtctc gtgtgctagc agtattcttg
     1021 aagaaccttc gaccgacaaa aaagagattt ttgaatacgc ctggaaaaca atcgacgaaa
     1081 gatattcttt cttcgaaatg aaaaatattg attgggacgc agttcatgcc cgttttgaac
     1141 cgggaattac caactcgatg tcagacgaac agttttttga cacacttggc gtaatgctcg
     1201 acatactgaa agacggacac actggggtaa cttcgccatt taacagtcac ttctacattg
     1261 ggttctttac ccgtggaatt gaaaactacg accaccgttt aattgtcgac aattattacc
     1321 acagctgggg aaaccggaaa ggtagttttt accacaccgc actggccgaa aaccaagttg
     1381 gatatattgc ctacagcgat ttcagcaact cgtttaccga tgaagacctc gactatctgc
     1441 tcaccaaata caaagacacc aaagggat
//
LOCUS       DAOPUW010000569         1465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_99659_length_1465_cov_3.812057, whole genome shotgun sequence.
ACCESSION   DAOPUW010000569 DAOPUW010000000
VERSION     DAOPUW010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99659_length_1465_cov_3.812057"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PKJ43_08775"
     CDS             complement(<1..1033)
                     /locus_tag="PKJ43_08775"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HNZ72703.1"
                     /translation="MRKLSKLMIFSLILFSMLATSCEQKKKNLAIDPANLDTTVDPAQ
                     DFDNYANGGWKEKNPLPDDKSRFGTFDKLRDSAEKQLQTLFENITTTTHETGSYGQKI
                     ADMYNMGMDSATLEQQGREPILPLLSEIENIQTIEQVQEAISKSHANGTGVLFSFFSS
                     ADEKNSEWVIGQLYQGGLGLSDRDYYVKDDERSKEIRTEYVKHVAKMFELAGENNVNA
                     AKKASQVMAIETRLANASMTRLERRDPDKTYNKMKLDGLKELSAGFNWQQFFNTVNLP
                     EPGDINVCMPLFFKEMSAMMQEIAVDDWKAYLGWNILNASAPYLSSAFVDQDFEFYGK
                     AMKGQVENRA"
     gene            complement(1165..>1465)
                     /locus_tag="PKJ43_08780"
     CDS             complement(1165..>1465)
                     /locus_tag="PKJ43_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ72704.1"
                     /translation="VDKDWEETKINYSETKKPARIFPQPANDRIIIQDDRFNKTRTIG
                     IKIFSLSGSLLLDQQEKGGKSAISVSLNPGWPSGLYLISLTTDDKSYSQKMIINR"
BASE COUNT          338 a          294 c          314 g          519 t
ORIGIN      
        1 gtgcacgatt ttcaacctgc cctttcatcg cttttccata gaattcaaaa tcctgatcga
       61 cgaaggctga agaaagatag ggtgctgagg catttagtat gttccagcca agatatgctt
      121 tccagtcatc aacggcaatt tcttgcatca tggcactcat ttctttgaaa aataatggca
      181 tacaaacgtt tatgtcgccg ggttcaggaa ggttgacggt gttgaagaat tgttgccaat
      241 tgaatccggc agataattct ttaagcccgt cgagtttcat tttattgtag gttttatcgg
      301 gatcacgccg ttcaagcctt gtcatcgatg cgttggcaag ccgggtttcg atggccatta
      361 cctgcgatgc tttttttgcc gcatttacat tgttctcgcc agccagttcg aacattttgg
      421 caacatgttt cacatactca gtcctgattt ctttcgaccg ttcgtcatct tttacatagt
      481 agtcgcggtc gcttaggccc aagcctccct gataaagttg gccaataacc cattcgctgt
      541 tcttttcgtc ggcggaactg aaaaacgaaa atagcactcc tgttccattc gcatgagatt
      601 tgctgattgc ttcctgaact tgctcaatgg tttgaatatt ttcaatttcg gaaagcaagg
      661 gaagtatcgg ttctcgtcct tgttgttcga gagttgctga gtccatcccc atgttgtaca
      721 tgtcggctat tttttgtccg taactgcctg tttcatgcgt ggttgttgtg atattttcaa
      781 acagtgtctg aagctgtttt tctgccgaat cgcggagttt gtcgaatgtc ccaaacctgc
      841 ttttgtcgtc gggcaaggga tttttttctt tccaacctcc gttggcatag ttgtcgaagt
      901 cttgtgccgg gtcgaccgtt gtatctaaat tagccgggtc gatggctagg ttttttttct
      961 tttgctcgca cgatgtggct aacatcgaaa aaagaataag actgaaaatc attagtttcg
     1021 ataattttct catgaatatt aatttaatgt gtcgtgtaat ttgtgattgg gccacaaaga
     1081 taatccaaat gggttaggct ttaaaatatc ttatggcttg ttttcactgg ttattttgat
     1141 ttactatact atattttcct gtaattatct gtttatgatc attttctgtg aatatgattt
     1201 gtcgtcggta gttagcgaga ttaaatacaa cccgcttggc caaccaggat ttaggctaac
     1261 tgaaatggct gatttacctc ctttctcctg ttgatcgagt aatagtgaac ccgatagaga
     1321 aaatatttta atgccgatgg ttcgtgtttt gttaaaacga tcatcttgaa ttataattcg
     1381 atcgttggca ggctggggga agatcctcgc tggttttttt gtctcgctat agttgatttt
     1441 tgtttcttcc caatctttat ctacc
//
LOCUS       DAOPUW010000570         1465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_99662_length_1465_cov_3.543262, whole genome shotgun sequence.
ACCESSION   DAOPUW010000570 DAOPUW010000000
VERSION     DAOPUW010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99662_length_1465_cov_3.543262"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PKJ43_08785"
     CDS             <1..387
                     /locus_tag="PKJ43_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72705.1"
                     /translation="PTAGLDPRERIRFRNLLVELSRERIVIFSTHIIEDIASSCNQLA
                     VLRRGEVQYIGSPRNMTVEAQGRVWQLVVPEEEFETFAAQMHVVHHLKEGNNIRLRII
                     AATQPHPEAVSVEPNLEDAYLWLQKR"
     gene            422..1132
                     /locus_tag="PKJ43_08790"
     CDS             422..1132
                     /locus_tag="PKJ43_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72706.1"
                     /translation="MKIFETIKRQIVMIFYNLKIIFGNKFIYFLLGAVGLFLLITILN
                     LAQGQVLSPSDVYGILMLVGALLLFYPVAFGIQSDKDARTLEIIFGIPDYRFRVWFMR
                     VLLIYGITFLFVLLLGFLLKVSLTNFPLFNMAFSVMFPLFFMGFLTFFLATVTRSGNG
                     TAAIIIIISLVLMMFSSAIQNSQWNVFFNPWGNTNGTNAMVWANIVLKNRIFLMAGGV
                     VFMLAALLNLQKREKFLG"
     gene            1250..>1465
                     /locus_tag="PKJ43_08795"
     CDS             1250..>1465
                     /locus_tag="PKJ43_08795"
                     /inference="COORDINATES: protein motif:HMM:NF027556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4890 domain-containing protein"
                     /protein_id="HNZ72707.1"
                     /translation="MKFKALFTCLLIVVAVIASNAQPPQGGGFGQMDPEQMAKSQTDR
                     MAEDLKLDDKQKTEVQAINVKYSKKMGE"
BASE COUNT          416 a          271 c          320 g          458 t
ORIGIN      
        1 ccaacagcag ggctcgatcc gcgcgaacgt atccgtttcc gtaatttgtt ggtggaactg
       61 agccgtgagc gcattgtaat tttctcaaca cacattatcg aagatatagc cagttcttgt
      121 aaccaattag ctgtactgcg ccgaggcgaa gtgcaataca ttggatcacc ccgcaatatg
      181 acagttgagg cacaagggcg tgtttggcaa ctggttgttc ccgaagaaga atttgaaacc
      241 tttgctgctc aaatgcatgt agttcatcat ttgaaagaag gaaacaacat ccgcttgcgt
      301 attattgcag ctacccagcc gcaccccgag gcagtttcgg tagaaccgaa ccttgaagac
      361 gcatatttgt ggctgcaaaa acgatgaatg gaatacatgg taaacaatta aaaacaacga
      421 aatgaagata ttcgaaacca taaaacgaca gatcgtaatg attttctaca atctgaaaat
      481 catcttcgga aataagttta tttattttct gctgggagct gttggcctgt ttttgcttat
      541 caccatactg aatctggcgc aggggcaggt gctgtctccg tctgatgtat acggaatatt
      601 gatgctggtg ggtgccttgt tactttttta tcctgttgca tttggtattc agagcgacaa
      661 agatgcacgt accctcgaaa taatattcgg tattcccgac tataggtttc gggtgtggtt
      721 catgagggta ttacttattt atggcattac gtttttgttt gtgcttttgt tgggttttct
      781 gctcaaagtc agcttgacta attttccatt gttcaacatg gctttttcag taatgttccc
      841 tttgtttttc atgggattct taacattctt tctggcaacg gttacccgtt ctggtaacgg
      901 aacagcggca attattatca tcatctcgct tgttttgatg atgtttagta gcgctatcca
      961 aaacagtcag tggaacgtgt tttttaatcc ttggggaaat acaaacggca ctaatgcaat
     1021 ggtttgggca aatattgtgc tgaagaaccg catctttctg atggctggcg gagtagtttt
     1081 tatgttggct gcgttgctta atttgcaaaa acgtgaaaaa tttttgggat aatttgattt
     1141 taagattaaa ccaaatcact gttttttaat ctataaagag ttgcaagaca tcaacagtaa
     1201 tgtcacaaca agattttttt tgatgtacaa aatttaaaac acagttatta tgaaatttaa
     1261 agccttattt acatgtttat tgattgtcgt tgcagttatt gcatcgaatg cacaacctcc
     1321 acaaggcggt ggtttcgggc agatggatcc tgaacaaatg gccaaaagtc agaccgatcg
     1381 tatggctgaa gacttgaaat tggatgacaa acaaaaaacc gaagttcaag ccatcaatgt
     1441 aaaatactcg aaaaagatgg gcgag
//
LOCUS       DAOPUW010000571         1465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_99706_length_1465_cov_2.331206, whole genome shotgun sequence.
ACCESSION   DAOPUW010000571 DAOPUW010000000
VERSION     DAOPUW010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99706_length_1465_cov_2.331206"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PKJ43_08800"
     CDS             <1..588
                     /locus_tag="PKJ43_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNZ72708.1"
                     /translation="AISKKPQDTISKELKELHDQGNLSPEMVFRDPYFLNFLGLKDSY
                     SEKDLEKAILNQLEKFILEIGQGFTFVERQKRMVIDNEDYHLDLLFYHRKLKRLVAID
                     LKLGKFKASYKGQMELYLRYLEKFETEEGEKSPLGLILCSENSPEQIELLKLEESGIK
                     VAEYMTELPSKKLLEKKLHTAIEMAKKQIESRSNE"
     gene            581..>1465
                     /locus_tag="PKJ43_08805"
     CDS             581..>1465
                     /locus_tag="PKJ43_08805"
                     /inference="COORDINATES: protein motif:HMM:NF040245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNZ72709.1"
                     /translation="MSNVLTNYTATEELADLFYQNRSVYYEGSSPVMNEARELAMRKF
                     QEKGIPSRKNENYKYTDLRPAFQNDFSVVPKYIEQNVDLHDLFNCDVPQLDTHIILLV
                     NGWYYGRNRRVGELPQGVICCSLQHAAREHTEIFNKYYNQQAGQTDDPLPNLNTALAK
                     DGLFLYIPDNVTIEKPIQVINLLNAAENTFAVQRNLFVCGKNSEATIVFCDHTLNQNY
                     YILNNVSECVVDRDARFSFLSLQNQHNGAVNLTHLFGDVWENANLLTNTLTLNGGIVR
                     NNFSVKLVGEHASASLNGL"
BASE COUNT          459 a          278 c          318 g          410 t
ORIGIN      
        1 gccatttcga agaaacctca agatacaatc tccaaagagt taaaagaatt acatgatcag
       61 ggtaaccttt cgcctgaaat ggtttttcgc gatccttatt ttttgaattt cctggggttg
      121 aaagattcat atagcgaaaa agatttagaa aaagctattc tgaatcaatt ggaaaaattc
      181 attcttgaga ttggacaggg ttttactttt gtagagcgtc agaaaaggat ggttattgac
      241 aatgaagatt atcatcttga tttactattc tatcatcgaa aactcaaaag attagtggcg
      301 attgatttga aattgggaaa attcaaggca agttataaag gccagatgga gctatatctt
      361 cggtatctcg aaaaatttga aaccgaagaa ggggaaaaat cgccattggg tttgatctta
      421 tgttctgaga attcaccaga gcaaattgag ttgctgaagt tagaagagtc gggaataaaa
      481 gttgcagagt atatgacaga gcttccctcg aagaaattat tggaaaagaa gttgcataca
      541 gccattgaaa tggcaaaaaa acaaatagaa agcagaagca atgagtaatg ttttaaccaa
      601 ttatacggct actgaagaac tggccgatct tttttaccag aaccgatcgg tatattacga
      661 gggttcttct cccgtaatga acgaggcgag agagctggca atgcgcaaat ttcaggagaa
      721 aggtattcct tcgcgcaaaa acgaaaatta taaatacacc gacctgcgtc ctgcatttca
      781 aaacgacttt tcggttgtac caaaatatat cgagcagaat gtcgacctgc acgacctttt
      841 taattgtgac gtaccccagt tggataccca cattatatta ttggttaatg gttggtatta
      901 cggacgtaat cgtcgggttg gcgagttacc acaaggtgta atttgctgca gccttcaaca
      961 tgccgctcgg gaacataccg aaatcttcaa taaatattat aaccagcaag ctggccagac
     1021 tgacgatccg ctgccgaacc taaacacagc tttggccaaa gacgggcttt tcctgtacat
     1081 tcccgacaat gttacgatcg aaaaaccgat tcaggtgatt aaccttctga atgcggccga
     1141 gaatactttt gccgtgcagc gaaatttgtt tgtgtgtgga aaaaacagtg aggcaactat
     1201 tgttttttgt gaccatacgc tcaaccagaa ctattacatt ctgaacaatg tcagcgaatg
     1261 tgttgtcgat cgcgatgccc gctttagttt cctgtcttta caaaaccagc ataatggtgc
     1321 cgtcaacctg actcaccttt ttggcgatgt ttgggagaat gccaatctgc ttaccaatac
     1381 attaaccctg aatggcggaa ttgttcgtaa caacttttcg gtaaaattgg ttggtgaaca
     1441 cgccagtgcc agtttgaacg gcttg
//
LOCUS       DAOPUW010000572         1464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_99796_length_1464_cov_2.713982, whole genome shotgun sequence.
ACCESSION   DAOPUW010000572 DAOPUW010000000
VERSION     DAOPUW010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99796_length_1464_cov_2.713982"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /gene="mreB"
                     /locus_tag="PKJ43_08810"
     CDS             complement(<1..620)
                     /gene="mreB"
                     /locus_tag="PKJ43_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HNZ72710.1"
                     /translation="MGLFSFLTQEVAVDLGTANTIIIHNDKIVVDEPSIVAIDLKTDK
                     MVAIGDKARQMQGKTHANLKTIRPLRDGVIADFNAAEQMIRGMIKMINTGSRLFPPSL
                     KMVVCIPSGSTEVELRAVRDSSEHAGAREVYLIYEPMAAALGIGIDVEAPEGNMIVDI
                     GGGTTEIAVISLGGIVTNKSIRIAGDDLTADIMEYMRHQHNIKVGER"
     gene            complement(626..>1464)
                     /locus_tag="PKJ43_08815"
     CDS             complement(626..>1464)
                     /locus_tag="PKJ43_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase PurH"
                     /protein_id="HNZ72711.1"
                     /translation="EDIFDQLHGKEISYNNLLDIDAAVNLIDEFSEPTFAILKHNNAC
                     GCASRENLLQAWKDALAGDPVSAFGGIIITNRTIDVETANEINKLFFEVIIAPSYGDG
                     VVDIFQSKKNRIILVRKNAKTANYQFRSVLNGVLYQDRDLKQEKADELKPVTNLEPTD
                     KQIEDLLFANKLVKQSKSNTIVLAKNKQLIASGVGETSRVDALKHAIEKAESFGFDLK
                     GAVMSSDAYFPFADSVEIAHGVGVDCVIQPGGSVRDQDTIDFCNDHGMAMVFTGTRHF
                     KH"
BASE COUNT          400 a          331 c          276 g          457 t
ORIGIN      
        1 cgttcaccta cttttatatt gtgctgatgg cgcatatatt ccatgatatc agcagttaaa
       61 tcgtcgccag caatcctgat tgatttgtta gttacaattc cgccaagcga tattactgca
      121 atctcagtag ttccgccacc gatatcaaca atcatgtttc cctcgggtgc ttcaacatca
      181 attccaatac ccagcgcagc agccatgggt tcataaatga gataaacttc gcgggctccg
      241 gcatgttctg atgaatcgcg aacggccctg agttcaactt cagtactgcc tgaagggatg
      301 caaaccacca ttttcaacga tggtggaaat aaccggcttc ctgtgttgat catctttatc
      361 atgccgcgta tcatttgctc ggcagcatta aaatcagcaa taactccatc acgtaacggc
      421 ctgattgttt tgaggtttgc gtgagttttg ccttgcattt ggcgggcttt atcgccaatt
      481 gcaaccattt tatcggtttt cagatcgatc gctacaatcg acggctcatc aacaacaatt
      541 ttatcgttat gaataattat tgtgttcgca gtacccaagt caactgctac ttcctgtgtc
      601 aaaaatgaaa ataatcccat ttgagttagt gtttaaaatg acgtgttcct gtgaatacca
      661 tagccattcc atggtcattg caaaaatcga ttgtatcctg atcacgaact gatccacctg
      721 gctgtattac acagtcaacc cctaccccat gtgctatttc aacactatcg gcaaaaggga
      781 aataagcgtc agatgacatc actgctcctt taaggtcgaa tccaaaactt tctgcttttt
      841 caatggcatg tttaagtgca tcaacgcgtg aggtttctcc aacgccagag gcaatgagtt
      901 gtttgttctt ggcaaggaca attgtatttg atttcgattg ttttaccaat ttgttggcaa
      961 acagcaaatc ttcaatttgt ttatcggttg gttcaaggtt ggtcacaggc ttcagttcat
     1021 cggccttttc ctgtttcaag tccctgtctt gataaagcac tccgttcaac acactacgga
     1081 attggtaatt agctgttttt gcatttttcc gaactaaaat aatccggttt ttcttcgatt
     1141 gaaaaatatc aaccacccca tcgccataag acggagcaat aataacttca aagaaaagct
     1201 tatttatttc gttggctgtt tctacatcaa ttgtccggtt agtaataata atgcctccaa
     1261 acgcagaaac tgggtcgccg gcaagtgcat ctttccaagc ttgaagaaga ttctcgcgcg
     1321 aagcgcaacc gcaagcatta ttgtgtttta aaattgcaaa agttggttca ctgaattcat
     1381 caatcaagtt aaccgccgca tcaatgtcga gtagattatt gtatgatatt tctttaccat
     1441 gcaattggtc aaaaatgtct tcaa
//
LOCUS       DAOPUW010000573         1464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_99817_length_1464_cov_2.210788, whole genome shotgun sequence.
ACCESSION   DAOPUW010000573 DAOPUW010000000
VERSION     DAOPUW010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99817_length_1464_cov_2.210788"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PKJ43_08820"
     CDS             <1..363
                     /locus_tag="PKJ43_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72712.1"
                     /translation="PKSILMGEKWKLKGFETDDDDFAEIFNLMFSLTNATYEFKKDGV
                     YVVTMKVLFVTESEKGTWSISDDGKELTLDGEVSEIKELTKSVLKLGPNKSLMGDYAD
                     GDDDIEAFTDYTIVFDAK"
     gene            complement(446..1258)
                     /gene="ispE"
                     /locus_tag="PKJ43_08825"
     CDS             complement(446..1258)
                     /gene="ispE"
                     /locus_tag="PKJ43_08825"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNZ72713.1"
                     /translation="MICYPNAKINIGLEVIRKRDDGFHDIETVFYPLSLSDILEMNVS
                     ANTSLTLSGIDIVDDPNNNLVLKAYRLLKNDFPLPPVSFHLHKQIPTGAGLGGGSADG
                     AYTLKGLNALFNLNIDTEKLENYASQLGSDCAFFIQNKPVFAEGRGNIFTTIDLSLKG
                     NWLVLVKPACAVSTAQAYGGVKPEVPGSKLTELVKMPVSEWGGKVVNRFEESVFSIFP
                     EIGKIKQTLLDNGAVYAAMSGSGSTVYGIFEQVPNELKTQFQNYFYHQECFR"
BASE COUNT          455 a          293 c          282 g          434 t
ORIGIN      
        1 cctaaaagta ttttaatggg cgaaaaatgg aaactgaaag gtttcgagac agacgatgat
       61 gattttgctg aaatcttcaa tcttatgttt tcgctcacta atgctactta tgaattcaaa
      121 aaggatggcg tttatgttgt cactatgaag gtcttatttg tgactgaatc ggaaaaagga
      181 acttggagca ttagcgacga tggcaaagaa ctcactctcg atggcgaagt gtctgaaatt
      241 aaggaattaa ccaaaagtgt attaaaactg ggaccaaaca aatcacttat gggagattac
      301 gccgatggtg atgacgatat tgaagctttt accgattaca ctattgtgtt tgatgcaaaa
      361 taaaaacatt cttaaataaa tggaataccc aattgaggag ttgattgata atcacttgca
      421 attgggtttt cttatgtagg gttgattacc taaagcattc ttgatgataa aaatagttct
      481 gaaactgtgt tttaagctca tttggaactt gctcgaaaat tccataaaca gttgacccgc
      541 tccccgacat ggcagcatac accgcaccgt tatcaagcaa agtttgctta attttcccga
      601 tttcagggaa tatactgaaa acactttcct cgaaccggtt aactaccttt cctccccact
      661 ctgaaacggg catttttacg agctctgtta gcttcgaacc gggtacttca ggtttaaccc
      721 cgccgtaagc ttgtgctgtt gatactgcgc aagctggttt caccaataca agccaattgc
      781 ctttcaggct taagtcaatc gttgtgaaaa tatttcctct tccttcagca aaaaccggct
      841 tgttctggat gaaaaaagcg caatcgctgc ccaattgcga tgcataattt tctaatttct
      901 ccgtatcgat gtttaaattg aagagggcat taagtccttt taacgtgtat gccccatcgg
      961 ccgatccacc accaaggccc gctccggtgg gaatttgctt gtgcaagtga aagcttaccg
     1021 gcggcaacgg aaaatcattc tttaagagac gatatgcttt caaaaccaag ttattgttcg
     1081 gatcatctac aatatctatc cctgaaaggg taaggctagt atttgccgaa acgttcattt
     1141 ccaggatgtc tgaaagcgac aatggataaa aaacagtttc aatatcatga aagccgtcgt
     1201 cccttttcct gatgacttcg agcccgatat ttatttttgc attgggataa caaatcataa
     1261 gcacacttgt aaaacaaggt gaaagaaata aaaaaaccaa tgaaaaggaa atattcttta
     1321 gaaatgattg aaccctgaat aagaaacgaa caaattactg ctcttttact ccattttttg
     1381 ttctcttttt tagctccgaa gcgtatgcta tccctactgc aataatgaac actccaaggg
     1441 caacaagtat gtataccata gttt
//
LOCUS       DAOPUW010000574         1462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_100003_length_1462_cov_2.685856, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000574 DAOPUW010000000
VERSION     DAOPUW010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100003_length_1462_cov_2.685856"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..580)
                     /locus_tag="PKJ43_08830"
     CDS             complement(179..580)
                     /locus_tag="PKJ43_08830"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNZ72714.1"
                     /translation="MKNTTKDWLSAAQDDIKSMSLLLKNPDLTNIVAFHAQQAIEKSL
                     KALMEESEIEFIKTHNLKTLLASTNTNLNINKRVFTELDQLYLDSRYPGDIGLMPFGK
                     PSMEDAQQYLDEAKKIYQEIEKRISTLKKDI"
     gene            complement(957..>1462)
                     /locus_tag="PKJ43_08835"
     CDS             complement(957..>1462)
                     /locus_tag="PKJ43_08835"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HNZ72715.1"
                     /translation="FISLFNAINKMAEKYDMPILYSCHPRSKKRIEESGFLLDKRVIR
                     HEPLGFHDYNCLQMNAAAVVSDSGTLPEESSFFTSIGHPFPAVCIRTSTERPEALDKA
                     IFTLAGISERSLLQAVDTAIQMNANGHHGLPVPDYVEENVSTKVVKIIQSYTDVVNKM
                     VWRKTNN"
BASE COUNT          368 a          264 c          321 g          509 t
ORIGIN      
        1 tattcactac ttcggtaaag ctttttttag aacacggatg acacgggttt gacggattta
       61 cacggattga atattaaaaa tcggtgacca tctgtttgat ctgtttgatc tgtgttcttt
      121 gtcgacagaa acgaagctct gcgtagctaa tgacaccttc tttattggtt gttggttgtc
      181 agatgtcttt ttttaaagtg ctaattcgtt tttctatttc ctggtatatt ttttttgctt
      241 cgtcaagata ttgctgtgca tcttccatcg atggtttacc gaatggcatc aacccaatat
      301 cgccagggta ccgggagtcc aggtataatt ggtccaattc tgtgaatacc cgcttgttaa
      361 tgtttaaatt ggtgtttgtt gaggctaaca gtgtttttaa gttatgtgtt ttaataaatt
      421 caatttcaga ttcttccatt aaagctttca aacttttttc aattgcttgc tgggcatgga
      481 atgcaacgat gttcgttagg tcaggatttt tcaaaagaag gctcatcgat tttatgtcat
      541 cctgtgcagc agaaagccaa tccttagttg tgtttttcat agataatttt tcctttagaa
      601 ttaatttcct ttgaaaacat actggatctt tcttgtaaaa ttttgaacat tgatttggtg
      661 tagatgatta agtcgatggg tattttcttt ctgaaatttt cgatgaaagg atcaatacga
      721 tggtataatt ccattttttc cctgaacgag tctggaatgt attcatctcc tgtaattact
      781 attaaatcta agtcactgtc cttatttggt gtaccgtaag catacgagcc gaaaaggatt
      841 attttttcgg gcttgctttt ttaagctctt cgacaattaa tggtatgtat tgttggtagt
      901 tcatctgttc aaagataagt aaaaagtaaa aagtaaaaag taaaaagact aatattttag
      961 ttgttggttt ttcgccaaac catcttgttt actacatcag tatagctctg aatgattttg
     1021 accactttgg ttgatacatt ttcctctaca taatcgggca cgggcagtcc gtggtggccg
     1081 ttggcgttca tttgtatcgc tgtatccacc gcttgcagta aggagcgttc gctaatgcct
     1141 gccaaggtga aaatggcttt atccaacgct tcggggcgtt cggtgcttgt acggatgcaa
     1201 acggcaggga aggggtggcc aatggaggtg aaaaaactgc tctcctcggg cagggtgccg
     1261 ctgtcggata ccactgcagc tgcattcatc tgcagacagt tgtaatcgtg aaaacccaaa
     1321 ggctcgtgcc ggatcaccct tttgtcgagc agaaagccac tctcctcaat ccgtttcttt
     1381 gagcggggat ggcacgagta gaggatgggc atatcgtact tttcggccat cttgttgatg
     1441 gcgttaaata gcgagataaa gt
//
LOCUS       DAOPUW010000575         1453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_100948_length_1453_cov_3.157368, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000575 DAOPUW010000000
VERSION     DAOPUW010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100948_length_1453_cov_3.157368"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="PKJ43_08840"
     CDS             complement(<1..930)
                     /locus_tag="PKJ43_08840"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNZ72716.1"
                     /translation="MGKHWLIILFLFGSYSIQAQKQLVAFNDSCLYYEGRILFHDKAA
                     ELSWPGTSVTIRFIGNELSATMVDSDTADYYNTIVDGKVHSIIHVGTEKKKYKLVSGL
                     TEGEHTVQLFKRTEWDKGKTLFYGFELPPEARVLPKLAPKKRKIEFYGNSITCGYANE
                     DAAGQDRWYGYFENNYEAYGAITARHFDANYSCIAKGGIGIMVSWFPLLMPEMYDRID
                     PTDSLSSWDFSKFSPDLVVINHFQNDSWLVHKPEHEQFIFRFGAKPPTETDIILAYKK
                     FVSSIREKYPNTPIICALGSMDATREGSPWPGYI"
     gene            complement(1020..>1453)
                     /locus_tag="PKJ43_08845"
     CDS             complement(1020..>1453)
                     /locus_tag="PKJ43_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72717.1"
                     /translation="IGLDAKLKFLNLIKSNKFDVNFLPNLRMYYKPNSRDYEVAADFR
                     FDMNLYDVITSARRKLMYNLQAGANVVYTRFYRPTLVIQPYVAFDFLNFKMYYTYQFN
                     PSLKIPGYWGRNQVAFVYQLGRDKFDRDSDKSSKPILRKKH"
BASE COUNT          448 a          295 c          276 g          434 t
ORIGIN      
        1 aatgtatccc ggccagggag agccttcgcg tgtggcatcc atactcccta aagcacaaat
       61 tatcggtgta tttggatact tttcacgaat actggaaaca aattttttat acgccagaat
      121 tatgtctgtt tccgtaggag gcttagcccc aaagcgaaaa ataaactgtt cgtgttctgg
      181 tttatggacc aaccatgaat cgttttgaaa atgattgatt actacaagat cgggggaaaa
      241 ctttgaaaaa tcccaagatg aaagagaatc agttgggtcg attctatcgt acatttcagg
      301 cattagcaac ggaaaccaac tcaccataat gccaattcca ccttttgcga tgcaagaata
      361 attggcatcg aaatgtctcg cagttattgc gccatatgcc tcatagttgt tttcgaaata
      421 tccataccaa cgatcttgcc cggcagcatc ttcattggca taaccacaag taatggagtt
      481 tccataaaac tcaattttcc gtttttttgg cgccaattta ggcaataccc gggcttctgg
      541 aggtagctca aacccataga aaagagtttt tcctttatcc cattctgtgc gtttaaaaag
      601 ctgaacagta tgttctcctt ctgtcaatcc cgaaactagt ttgtactttt tcttttcagt
      661 cccaacatga ataattgaat gcactttccc gtcaacaatt gtattataat aatcggccgt
      721 atcggagtcg accatcgtag ctgaaagttc attcccaata aaacgaatcg taacgcttgt
      781 gcccggccat gaaagttcgg ctgctttgtc atgaaataaa atccgccctt cataatacag
      841 gcaggaatcg ttgaacgcta ctaattgttt ttgggcttga atagaatacg agccaaagag
      901 aaatagaatg ataagccaat gttttcccat aataattaaa acaataaacg atgaaatatc
      961 aataccggtt ttacattaaa atgaaggctg acaaattgcc agccttcgaa tatgttttat
     1021 tagtgtttct tacgtaagat tggtttactg ctcttgtctg aatcacgatc aaacttgtcg
     1081 cgtccaagct ggtaaacaaa ggccacttgg tttcgtcccc aatacccagg aatttttaag
     1141 cttgggttaa actggtaggt gtaatacatt ttgaagttca aaaaatcgaa agccacataa
     1201 ggttgaataa ccaatgttgg gcggtaaaaa cgagtataca ccacgttggc acctgcctgt
     1261 aagttataca tcagttttct tcttgcagag gtaataacgt catacaaatt catatcgaaa
     1321 cggaaatctg cagcaacttc gtagtcgcgt gaatttggtt tataatacat gcgaagattt
     1381 ggtaaaaagt tcacatcaaa tttattcgac ttgatcaggt taaggaattt caattttgca
     1441 tcgagcccta tag
//
LOCUS       DAOPUW010000576         1451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_101165_length_1451_cov_2.577364, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000576 DAOPUW010000000
VERSION     DAOPUW010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101165_length_1451_cov_2.577364"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="PKJ43_08850"
     CDS             complement(<1..1018)
                     /locus_tag="PKJ43_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNZ72718.1"
                     /translation="MVSVEQVVLNFGGFELFKGISFFINPRDRVGLTGRNGAGKTTLL
                     KIMAGIQQPTEGNVVTPKGTTIGYLPQTMILSDLRTVFDEAETAFSELLEIDKKIGQI
                     NLELSERTDYESIEYHTLIQKLTDLTERFNMLGGHSVHADIEQTLLGLGFSRNDFHRS
                     TSEFSGGWRMRIELAKLILRKPDVFLLDEPTNHLDIESIQWLEEFLKNYNGAIVLVSH
                     DKAFLDNVTNRTIEIVLGKIYDYKANYSQYVVLRKERREQQLAAFRNQQKMIQDTEDF
                     IERFRYKATKAVQVQSRIKQLEKIEVLEVDEEDVRQLNIKFQPAVRSGKIVAEIKGLS
                     KAYGT"
     assembly_gap    1083..1092
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1157..>1451)
                     /locus_tag="PKJ43_08855"
     CDS             complement(1157..>1451)
                     /locus_tag="PKJ43_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72719.1"
                     /translation="KENAEKRAELRMQKINEVCNLTPQQQAEVKQLFMQKEGKDNKQL
                     QKGNKQALAAARKAENQQFQSSLKQVLTPEQQTKWQGFKQAQKASRPAAIKKK"
BASE COUNT          328 a          296 c          324 g          493 t
ORIGIN      
        1 gtgtcccgta tgctttgctg agacctttaa tttcggcaac gattttaccc gatctaacgg
       61 cgggctgaaa cttaatgttt agctgcctta catcttcttc atcaacttcg aggacctcaa
      121 ttttttcgag ttgcttaatc ctggattgaa cctgtacggc ctttgtggct ttgtacctga
      181 agcgctctat gaaatcttca gtgtcctgaa tcattttctg ttgatttcgg aacgcggcaa
      241 gttgttgctc acgccgctct tttcggagaa caacatattg agaataattc gctttgtagt
      301 cgtatatttt gccaagtaca atttcaatag tacggttcgt tacattgtcg aggaaagctt
      361 tgtcgtgcga aacgagcacg atggcgccgt tataattttt caaaaactct tcaagccact
      421 gaattgattc aatgtcgagg tggttggtag gctcatcgag aaggaagaca tcgggcttgc
      481 gcaaaatgag ttttgccaat tcgatacgca tgcgccaccc accactgaat tctgatgttg
      541 agcggtggaa atcgtttctc gaaaacccca ggccaagtag ggtttgttca atatcggcat
      601 gcacagaatg accgccgagc atgttgaacc gctcggtaag gtcggtgagt ttttgtatta
      661 gtgtgtgata ttctattgat tcataatcgg ttctctcgga gagttccagg tttatttggc
      721 caattttctt gtcaatctcg agcaattcac tgaaagctgt ttcggcctcg tcgaacactg
      781 tgcgtaaatc tgagagtatc attgtttgag gcagatagcc aatagttgtt ccttttgggg
      841 taaccacatt cccttcggtt ggttgttgaa tgcctgccat aatttttaaa agagtggttt
      901 tcccggcacc atttcgtcct gttaaaccta ccctgtcgcg cgggttgata aagaaggaaa
      961 ttcctttaaa taattcaaaa ccaccaaagt ttaatactac ttgttctaca gaaaccattg
     1021 aaaattattt tggcggcaaa gatacaagtt tcgtttgaac tattctggca gattgtgggt
     1081 ttnnnnnnnn nncaatcaaa taaaaaacgc aaccccaaat tttggaattg cgctttattt
     1141 atatggtaag gtctgcttac ttctttttta tggctgctgg gcgtgaagct ttttgtgcct
     1201 gtttgaatcc ctgccatttg gtttgttgct cgggagtgag aacttgtttc aacgagcttt
     1261 gaaactgctg gttttcggct ttgcgggcag cggctaatgc ttgcttgttg cctttttgaa
     1321 gctgtttgtt atccttgccc tctttttgca tgaaaagctg ttttacctcg gcctgttgtt
     1381 gtggggtgag gttacacact tcgtttattt tctgcatgcg caattctgcc cttttttcgg
     1441 cattttcttt t
//
LOCUS       DAOPUW010000577         1449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_101417_length_1449_cov_2.928981, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000577 DAOPUW010000000
VERSION     DAOPUW010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101417_length_1449_cov_2.928981"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="PKJ43_08860"
     CDS             <1..1028
                     /locus_tag="PKJ43_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HNZ72720.1"
                     /translation="NKRGFASDNNSGVSPNILKALENVNEGHVTGYGTDIYTKKAIEI
                     FYKLFGFNIDVYFVFNGTGANILGLSTLSQPFHSILCPATAHIMTDECGAPERFTGCK
                     VIPIETPDGKLTAELIKPHLYGFGFEHHSQPGIISISQVTELGTLYQIDQIKEICQLA
                     HQHGMYVHMDGARIANAAAALNVNVTDFTRDAGVDVLSFGGTKNGMMFGEAVVFFNHD
                     LSENAKYLRKQSAQLFSKMRYIAAQFIGYFENNLWLDNARHSNEMAQYLAQKVKALGV
                     EITQKVEANGVFAILDPEISETLRNEYFFYDWDENKHEVRWMTSFDTRPEDIDGFCEH
                     LAILLHA"
     gene            1021..>1449
                     /locus_tag="PKJ43_08865"
     CDS             1021..>1449
                     /locus_tag="PKJ43_08865"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNZ72721.1"
                     /translation="MPKFTFRYYPQPLSPEEEELEKKIFRYSLVIPFIFLLIIWLIKI
                     AEVMSGEQYISLGVLPRNASGLIGIITSPLIHSDFKHLIANSSSFFVLSVALFYFYRK
                     VALKIFLLNYFLAGAFLWLGGRELIYIGASGIIYGIAAFLL"
BASE COUNT          429 a          325 c          284 g          411 t
ORIGIN      
        1 tgaataaaag aggatttgca agcgacaata attcaggcgt aagcccaaac atacttaagg
       61 cactggaaaa tgtcaacgaa gggcatgtaa caggctatgg aactgatata tatacaaaaa
      121 aagcgatcga gattttctat aaactttttg gcttcaatat cgatgtttat tttgttttca
      181 acggaaccgg tgccaacatt cttgggttgt ctaccctctc ccagcctttt cattcaatcc
      241 tttgtccggc aacagcgcac ataatgaccg acgaatgcgg tgcacccgag cgctttaccg
      301 ggtgcaaagt gatccccatc gaaacccccg atggtaaact cacagcagaa ctcatcaaac
      361 ctcaccttta tggatttggt tttgaacatc attctcaacc tggcatcatt tccatttcac
      421 aggtcaccga gcttggcacg ctttatcaaa ttgaccagat caaagaaata tgccaacttg
      481 ctcatcaaca cggcatgtat gttcacatgg acggggcaag aattgccaat gccgctgctg
      541 cattgaatgt aaatgtaaca gattttacac gcgatgccgg agttgacgtt ttatcgtttg
      601 gcgggacaaa aaacggaatg atgtttggtg aagccgttgt ctttttcaat catgatttat
      661 cagaaaatgc gaaatatttg cgaaaacaat cggcacagct tttctcaaaa atgcgataca
      721 ttgcagccca gtttattggc tacttcgaga acaacctctg gctcgacaac gctcgacatt
      781 caaacgaaat ggcccaatat cttgcgcaaa aagtaaaagc cttaggagta gaaataaccc
      841 agaaagtaga agcaaacgga gtttttgcca tcctcgatcc tgaaatcagc gaaaccttac
      901 gaaacgaata tttcttttac gattgggatg aaaacaaaca cgaagtacgt tggatgacct
      961 cgttcgatac caggcccgaa gacattgatg ggttctgcga acatcttgca attttgctac
     1021 atgcctaagt ttacattccg atattacccg caaccactct ctcccgaaga agaagaactg
     1081 gaaaagaaga tcttcaggta tagccttgtt atccctttta tttttttatt gataatatgg
     1141 cttataaaaa ttgcagaagt catgtcgggc gaacaatata tatccttagg tgtattgcca
     1201 cgcaacgctt caggcttaat cggaataatt acatcgcccc tcattcactc cgactttaaa
     1261 catctcattg ccaattcatc atcttttttt gtactatcag tagccttgtt ttacttctac
     1321 agaaaagttg ccctgaaaat attccttctc aattactttc ttgccggggc atttctctgg
     1381 ctcggaggcc gggaactcat ttacattggg gcgagcggga taatatatgg tattgcagca
     1441 tttttgctt
//
LOCUS       DAOPUW010000578         1447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_101660_length_1447_cov_2.474856, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000578 DAOPUW010000000
VERSION     DAOPUW010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101660_length_1447_cov_2.474856"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1447
                     /locus_tag="PKJ43_08870"
     CDS             <1..>1447
                     /locus_tag="PKJ43_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HNZ72722.1"
                     /translation="IFITGGENQNDLEKKLFLVRKKIEKATLQSSLKQKRAFYIVSLS
                     TRRMVYKGMLTSLQLREYYPDLTNANFTSGLALVHSRFSTNTFPTWDLAQPFRLLGHN
                     GEINTIRGNRYWIEARESILKSEQLGDINEILPLVQPGMSDSASLDNILEFLVMSGKT
                     LPHALAMLIPESWNDKNPISNDLKSFYEYHSILMEPWDGPATLLFSDGRYAGGLLDRN
                     GLRPARYLITKDDMIVVASEVGVIPFEASRIKEKGRLKPGKMLMVDTEKGEVYYDAEL
                     KETLAKAHPYSEWLSKNRISLEDISSGRNVKNNVENFEKQLKVFDYSKEDVEKLIIPM
                     ASEGKEPISSMGNDTPLAVLSNKPQLLFTYFRQLFAQVTNPPIDPIREELVMSLTLYI
                     GAQNGNLLEASPDLCKMVKLSSPILTNTEFDILKNLRYKGFKALTLPMLFDPSEGPAA
                     LEKAVDELCHKAEQAVNDEYHYIILSDRGVDE"
BASE COUNT          472 a          322 c          303 g          350 t
ORIGIN      
        1 atttttataa ctggcggaga aaaccagaac gacttagaaa aaaagctttt ccttgtcaga
       61 aaaaaaattg aaaaagccac gttacaatca tcgttaaaac aaaaacgggc attttacatc
      121 gtttcacttt cgacaaggag aatggtgtac aaaggcatgt taacctcgct gcaacttcgc
      181 gaatattacc ccgaccttac caacgccaat ttcacaagtg gattagcatt ggtacactca
      241 cgctttagta caaacacatt cccaacatgg gatttggcac aacctttcag gctgctaggg
      301 cacaacggcg aaatcaacac catacgcgga aaccgttatt ggatcgaagc ccgtgaaagc
      361 atcttaaaat cggaacaatt gggcgatatc aatgaaattc tcccattggt acaaccagga
      421 atgagcgaca gtgcttcgct cgacaatatt cttgaattcc ttgtgatgtc aggtaaaaca
      481 ttacctcacg cattggccat gttgattcca gaaagctgga acgacaaaaa cccaatttca
      541 aacgatttga aatcattcta tgaataccat agtatattga tggagccatg ggacgggccg
      601 gcaactcttc tgttttcaga cggacgatat gcaggaggtt tacttgaccg caatggactg
      661 cgcccagctc gttatttgat caccaaagac gacatgattg ttgttgcttc agaagtaggg
      721 gtaatccctt tcgaagcttc acgaattaaa gaaaaaggcc gtttaaaacc cggcaaaatg
      781 ttgatggtcg acaccgaaaa aggcgaggtt tattacgatg ccgaattgaa agaaacctta
      841 gcaaaagccc atccatacag cgagtggtta agtaagaacc gtattagtct cgaagatatt
      901 tcatcgggac gaaacgtaaa aaacaatgtc gaaaactttg aaaaacaact aaaggtattc
      961 gattacagca aagaagatgt cgaaaagtta atcatcccta tggcaagcga aggaaaagag
     1021 ccaatttcgt cgatggggaa cgacactcct cttgcagtat tgtcgaacaa accgcagttg
     1081 cttttcacat acttccgcca gttgtttgcg caggtaacca acccgccaat cgacccaatc
     1141 cgtgaagaat tggtcatgtc gttaacactt tacattggcg cacaaaatgg taatctgctc
     1201 gaggcatcgc ccgatttatg caagatggtt aaattatcga gcccgatctt gaccaatacc
     1261 gaattcgaca ttcttaaaaa cctccgttac aaagggttca aagcgttaac attgcctatg
     1321 ctgtttgacc cttcggaagg cccggccgca ttggaaaaag cggttgacga actttgccac
     1381 aaagcagaac aagccgttaa cgacgaatat cactatatca ttttaagcga caggggtgtt
     1441 gacgaaa
//
LOCUS       DAOPUW010000579         1445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_101857_length_1445_cov_2.636691, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000579 DAOPUW010000000
VERSION     DAOPUW010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101857_length_1445_cov_2.636691"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1445)
                     /locus_tag="PKJ43_08875"
     CDS             complement(<1..>1445)
                     /locus_tag="PKJ43_08875"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNZ72723.1"
                     /translation="GLIYDRNGEMLVYNQIVYDLLATPNEIGQFDTITLCSIIDVPKE
                     VFTDELKKAKGKSTYRPSVIIKQIPPDRYALLKEKMYKYPGFYLQTRNVRTYPFRCAA
                     HVLGYIGEVSPAFLKKNPYYRSGDYIGVSGIEKAYEEHLRGRKGVSLFLVDVHGRIKG
                     SYEDGKLDTVAVKGRDLVSSLDRELQQYGEKLMVNKAGSIVAIEPSTGEILSLVSSPS
                     YDPVEMVGRNRTVNFPRLLNDPLLPLFNRAISAQYPPGSTVKMLHGLIALQENIISLG
                     SSYYCAHGFHVGNFHQACHHEQSFNLNNAIAQSCNAYFSQEFRDLLQDKKYGGARNGY
                     EVWRNHVLSFGFGNRISYEFDEEAKGFIPEAEYYEKRVFKNAKWRALPIISLAIGQGE
                     MQTTPLQMANYTAILANRGFYYPPHVVRSIDGAELNPELKTKRYTTVNSIQFERIMDG
                     MEEVVNSGTASIARIPGITMCGKTGTAQNPH"
BASE COUNT          403 a          337 c          272 g          433 t
ORIGIN      
        1 atgagggttt tgtgccgttc ccgtttttcc acacatagta attcccggta tacgtgcaat
       61 actggctgtt ccggagttta caacctcttc catgccatcc ataatccttt cgaattgaat
      121 tgaattcacc gttgtatacc gtttggtttt cagctctggg tttaactcag ccccatcaat
      181 agaccgcaca acatgaggcg gataataaaa gcctctgttt gcgagtattg cagtgtaatt
      241 agccatttgc aaaggagtgg tttgcatttc tccctggcca atagctaatg aaataatggg
      301 caaagccctc catttcgcat ttttaaatac tcttttttca tagtattcag cttctggaat
      361 aaagccttta gcttcctcat caaactcata tgagattcgg tttccaaacc caaaactcaa
      421 tacgtggtta cgccaaactt catatccatt tcgggcacct ccatattttt tatcttgaag
      481 caaatctctg aactcttgtg aaaaatatgc attacaagac tgtgctattg cattattaag
      541 attaaacgat tgttcgtgat ggcatgcctg atgaaagttc cctacatgaa aaccatgcgc
      601 acaataatat gaagacccca acgaaataat attttcttgc agggcgatca atccatgtaa
      661 cattttcaca gtcgacccag gagggtattg cgcacttatt gcccggttaa acaacggcag
      721 caacgggtcg tttagcaagc gggggaaatt taccgttcgg tttcgtccga ccatttcaac
      781 aggatcgtag gaaggcgaac tcaccagcga caaaatctcg cctgttgatg gttcaatggc
      841 gacaatactc ccggctttat tcaccattag tttttcgcca tattgttgca attcacggtc
      901 gagagaagaa actaaatccc tccctttcac agccactgta tctaacttgc catcttcgta
      961 agagcctttt atacgcccgt gtacatcgac taaaaaaagc gaaactcctt ttcggcctct
     1021 caagtgttct tcataggcct tttcaatacc cgaaaccccg atatagtcgc ccgatcgata
     1081 ataagggttt ttctttaaga aagccgggct aacttcgcca atatagccta acacatgggc
     1141 agcacaccga aaagggtaag ttctcacatt acgggtttga aggtaaaacc cgggatattt
     1201 atacattttt tctttcaaca aagcatacct gtcggggggt atctgtttaa ttatgaccga
     1261 aggcctgtaa gtcgactttc ctttagcttt tttaagttca tcggtaaaaa cctctttagg
     1321 aacatcaatt attgagcaaa gagtaatcgt atcgaattgt ccgatttcat ttggagtggc
     1381 aagcaagtca taaactatct gattgtaaac caacatttct ccgttacggt cgtatatcag
     1441 ccccc
//
LOCUS       DAOPUW010000580         1443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_102056_length_1443_cov_3.662824, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000580 DAOPUW010000000
VERSION     DAOPUW010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102056_length_1443_cov_3.662824"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..>1443
                     /locus_tag="PKJ43_08880"
     CDS             46..>1443
                     /locus_tag="PKJ43_08880"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HNZ72724.1"
                     /translation="MSVSLIILTIAGASALFLYGLKLMSESLQKIMGDWLQIMLGSIS
                     SNRAKGLMTGVFITALIQSSSATTVMVVSFVNAGILRLSEAVGVIMGANIGTTLTAWI
                     VSFFGFQIDISLLVLPLIGLGIPLIFSNNRNVKNWGEMLLGFSLIFIGLGFLKQFIPD
                     AGASVLLEAINFLKSFEYGAYLVFLLIGAVFTAFTRSSNAVFAVIAVLCFKGWISFDL
                     AVSMILGINIGKTLSAIRASQIANTNARRAALAHFFYNLIGIFWGIILLPVLVKGVIF
                     LSVFVGIGNPSLEPQAVPLSLAMFHTLFNVVNTLVLFQFSRQLTKYIIKKIPLDSRGE
                     DEFKLTHIKTGMLSTPDASLFQAKREAIVFAESVRKMFMSVERIIFEKSEKEYLTLKE
                     DIGAKEIQADRLEKEIATFLTKVSEGRLSESSSRRLRALYEMIDDMESIADSCNNILN
                     IVERKKLKKIEFPEPI"
BASE COUNT          384 a          246 c          315 g          498 t
ORIGIN      
        1 taatctggtt tcgaatatta attttgtaca ttacaacaag agaatatgtc agtttcactt
       61 atcatattaa ctatagcagg agcttcagcg cttttccttt atggattgaa actgatgagc
      121 gaatcgttac agaaaattat gggcgattgg cttcagatta tgcttggttc aatatcatca
      181 aaccgtgcca aagggttgat gacaggagtt tttattaccg ctttgatcca gtcatcttct
      241 gcaactacgg taatggttgt tagttttgta aatgcaggta ttttgcgtct gtctgaggcc
      301 gttggggtta ttatgggggc aaatattgga accacactta ctgcatggat agtttctttt
      361 tttggatttc aaattgatat ttctcttttg gtacttcctt tgataggttt aggtattcct
      421 ttaattttct cgaacaacag aaatgttaag aactggggag agatgttgct gggcttttcg
      481 ctcattttta taggattggg ctttctaaaa caatttattc ccgacgctgg ggcatctgtt
      541 ttgctcgagg ccatcaattt tctgaaatca tttgaatacg gagcttatct tgtttttctt
      601 ttaattggag ctgttttcac tgcttttacc cggtcatcta atgctgtttt tgctgtaatc
      661 gcagtattgt gcttcaaggg ctggatatct ttcgatctgg ctgtttcaat gattcttgga
      721 attaatattg ggaaaacgct tagtgctatc agagcatcac aaatagccaa tacgaatgca
      781 cgtcgtgctg cgttggctca ttttttctac aatttaatcg gaattttttg gggaattatc
      841 cttttacccg ttttggttaa aggggtaatc tttctttcag tgtttgttgg tattggtaac
      901 ccttcgcttg agccacaagc agttcctttg tcattggcaa tgttccatac gttatttaat
      961 gtcgttaata cgttggtact cttccagttt tcgaggcagc ttaccaagta cattattaaa
     1021 aaaattcccc tcgattcaag aggcgaagac gaattcaaac ttacccatat taaaacagga
     1081 atgctttcaa ctcccgatgc ttcgttgttt caggctaaac gcgaagcgat tgtatttgct
     1141 gaaagtgtaa ggaagatgtt tatgagtgtt gagcgtatta ttttcgagaa atcggagaaa
     1201 gaatacttaa cgttgaagga agatattggg gcaaaagaaa ttcaggccga caggttggag
     1261 aaagagattg ctacatttct caccaaagtg agtgaaggcc ggttgagcga aagcagctcg
     1321 cgtcggttac gggcgcttta tgaaatgatt gacgatatgg aaagtattgc cgattcgtgc
     1381 aataacattc tcaacattgt tgaacggaaa aaacttaaaa agattgaatt ccctgagcca
     1441 att
//
LOCUS       DAOPUW010000581         1442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_102161_length_1442_cov_3.517664, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000581 DAOPUW010000000
VERSION     DAOPUW010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102161_length_1442_cov_3.517664"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="PKJ43_08885"
     CDS             complement(<1..1285)
                     /locus_tag="PKJ43_08885"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HNZ72725.1"
                     /translation="MTAEPNIPKKLFLLDAYALIYRSYFAFIKNPRITSKGLNTSAIY
                     GFVNSLEQVLNQEQPSHIAVAFDMHGDTFRHKMFAEYKANREAMPEDIRRAIPYVRQL
                     IEAYNIPIIELTGFEADDIIGTIATKASEDGFTTYMMTPDKDYAQLVDQRTFMYKPAK
                     GGNLAEVWGIEQVKENFEIEAPAQVIDILGLMGDSADNIPGCPGIGPKTAIKLISDYG
                     NIDGVYENIGQLKGKLKESLETYKDQVFLSRKLAVIDRQVPITIDYTQMKYEGPNNEA
                     LHKLFVELEFRTFTSKIQVATQAVQQEATQGSLFDQPGVAASPQIITTQLETSETKKH
                     QYYKVENAMQRASLRAELSVASAFCFDTETTGIDPHRADLVCISFSIKPHEAYCVILP
                     EEYDESYAIVQEFKTLFEDRSIMKVGQNMKFDMLML"
     gene            complement(1295..>1442)
                     /locus_tag="PKJ43_08890"
     CDS             complement(1295..>1442)
                     /locus_tag="PKJ43_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNZ72726.1"
                     /translation="VDFQPTCENLVVHFVQIILPLLPEGIKLKKVKLYETASSYAEWD
                     ADDN"
BASE COUNT          350 a          291 c          326 g          475 t
ORIGIN      
        1 caagcataag catgtcgaat ttcatgtttt gcccaacctt cataatcgac cgatcttcga
       61 ataaagtttt gaattcctgc acaatggcgt aggattcgtc atactcttcg ggcaaaatca
      121 cacaataggc ttcgtggggt tttattgaaa aagaaataca aaccaaatcg gctcggtgtg
      181 ggtcaattcc tgttgtttct gtgtcgaagc agaaggcgct tgcaacagat aattcagccc
      241 taagagaggc tcgttgcatg gcattttcta ctttataata ttggtgtttt ttcgtttccg
      301 aagtttcgag ttgtgtagtt attatttgtg ggctagcggc tacccctggc tggtcgaaaa
      361 gtgatccttg tgtcgcctct tgttgaactg cctgtgttgc cacttgtatc ttcgaagtaa
      421 atgttctgaa ctctagttcg acaaacaatt tgtgcagggc ttcattgttt ggcccttcgt
      481 atttcatttg ggtataatca atcgtaatag gaacctgacg atcgattact gcaagttttc
      541 gagataaaaa cacttgatct ttatatgttt caaggctttc tttgagtttg ccttttagct
      601 gcccaatatt ttcatacact ccatcaatgt tgccgtaatc ggagataagt tttatggctg
      661 ttttggggcc aattcccggg catcccggaa tgttatcagc actatcaccc atcagcccaa
      721 gaatgtcgat tacctgtgca ggggcttcaa tttcgaagtt ttcctttact tgttcaatgc
      781 cccatacttc ggctaagttg cctcctttgg ctggtttgta catgaatgtg cgctggtcaa
      841 ccaactgagc ataatcttta tcgggggtca tcatgtatgt ggtaaatcca tcttccgagg
      901 cttttgtggc tatggttccg attatatcgt cggcttcgaa tcctgtaagt tcgattattg
      961 gaatattgta tgcttcgata agttgccgga catacggaat ggcacgccgg atatcttctg
     1021 gcattgcttc gcggttggct ttgtattccg caaacatctt gtgcctgaaa gtgtcgccat
     1081 gcatgtcgaa tgcaactgca atgtggcttg gttgttcttg attaagaact tgttccagcg
     1141 agttgacaaa gccgtaaatg gccgatgtat tgagcccttt tgaagtgatt ctcgggtttt
     1201 ttatgaaggc aaaatacgaa cggtatatca gagcataggc atcaagaagg aaaagcttct
     1261 ttggaatgtt aggttcagct gtcatagtta gttgctagtt atcgtcggca tcccattcag
     1321 cataggagct ggccgtttcg tataatttca cttttttaag tttaattcct tcaggcagaa
     1381 gaggtaaaat aatttgcaca aagtgtacca caaggttctc acaggttggc tgaaaatcga
     1441 ca
//
LOCUS       DAOPUW010000582         1440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_102369_length_1440_cov_3.571119, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000582 DAOPUW010000000
VERSION     DAOPUW010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102369_length_1440_cov_3.571119"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..645)
                     /locus_tag="PKJ43_08895"
     CDS             complement(16..645)
                     /locus_tag="PKJ43_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNZ72727.1"
                     /translation="MRKRGKNKLHVGVIIFATVIIISFTVQCDKSEPEGSIKPPVTVE
                     KEIPFDKTPKLSEMVVYEVNPLVFGPDGTFSNITERLSDIKDLGVNVLWLMPIFPEGQ
                     LNGVGSPYAVQNYTEVAPSLGNITDLKQLVKEAHKLEMAVIIDWVANHTAWDNPWIEN
                     DSWYTKDSQGNIINPPGTNWQDVAELNYNNEEMRNEMIKSMKFWRSHKM"
     gene            complement(655..>1440)
                     /locus_tag="PKJ43_08900"
     CDS             complement(655..>1440)
                     /locus_tag="PKJ43_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72728.1"
                     /translation="KVGLSFAIVYEDRTIPEVIKYDNSTDRIIAAQTDMEYIEENYFS
                     NPQYINIEGKPLLLVFGPEEFHSPDEWDEILAAFKNKPCFITLNGTSWQTAPHSSGEY
                     IWVDNSSIDNKYGSINKFDVFIGGAYPGFNDFYNEGGWGTGFQWSIDFNQGQTLKQNL
                     QKAENSGASYVQLITWNDYGEGTMIEPTKEFGFSLLEIVQEFTGVGYNASHLGKVYDL
                     YQLRKSISRTDDKIKMLDQAFYYLVSLQHEKANQLIDSVKNIQ"
BASE COUNT          350 a          315 c          281 g          494 t
ORIGIN      
        1 cgtcccgcat aatcgttaca ttttgtgcga tctccagaat ttcatcgatt ttatcatttc
       61 gttgcgcatt tcttcgttgt tataattgag ctcggccaca tcttgccaat ttgttccagg
      121 tgggtttatt atatttcctt gtgagtcttt tgtataccac gaatcgtttt caatccatgg
      181 gttatcccat gcggtgtggt tggccaccca gtcgatgatt acagccatct cgagtttatg
      241 tgcctctttt accaattgtt tcaggtcggt aatatttcca agcgacgggg ctacttcagt
      301 atagttttgt actgcataag gtgacccaac cccgtttagc tgaccttcgg ggaaaattgg
      361 catcagccat agtacattta cgcccaggtc ttttatgtct gaaagtcgtt ctgttatatt
      421 gctaaatgtt ccatcgggcc caaataccaa aggattgacc tcgtaaacaa ccatttcgga
      481 caatttagga gtcttgtcga acgggatttc tttttcaact gttaccggtg gtttaattga
      541 tccttccggc tcacttttat cgcattggac agtgaacgaa attattatta ctgtggcaaa
      601 tattataacc ccaacgtgta gcttattttt ccctcttttc ctcattttcc tcacttattg
      661 tatattcttt acactgtcga tgagttgatt cgctttttca tgctgaagcg ataccagata
      721 ataaaatgcc tgatcgagca ttttaatttt atcatcggta cgactgatgc tttttctaag
      781 ttgatataaa tcatacactt ttcccagatg ggaagcattg tagcctactc cagtaaattc
      841 ctgaacaatt tcgagaagcg agaacccaaa ttcttttgtt ggttcaatca tggttccttc
      901 gccataatcg ttccatgtaa ttagctgcac atagctggca cctgaatttt cagctttttg
      961 taaattctgt ttcagggttt gtccctggtt aaaatcgatg ctccactgga agccagtgcc
     1021 ccagccgcct tcgttgtaga agtcgttgaa cccagggtaa gcgcctccaa tgaagacatc
     1081 gaatttattg atacttccat atttattgtc gattgagcta ttgtcgaccc aaatgtattc
     1141 ccctgatgaa tgtggggccg tttgccacga tgtaccgttg agcgttataa aacaaggctt
     1201 gttcttgaac gccgcgagta tttcgtccca ttcgtcgggc gaatgaaact cttcgggacc
     1261 aaaaacgagc aataatggtt tgccttcaat gttgatatat tgaggattgc tgaaataatt
     1321 ttcttcaata tactccatat ctgtttgtgc tgcaataatg cgatcggtac tgttgtcgta
     1381 cttaattact tcaggaatag tccggtcttc atacacgatg gcaaacgata atcccacttt
//
LOCUS       DAOPUW010000583         1436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_102781_length_1436_cov_3.426503, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000583 DAOPUW010000000
VERSION     DAOPUW010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102781_length_1436_cov_3.426503"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..>1436)
                     /locus_tag="PKJ43_08905"
     CDS             complement(185..>1436)
                     /locus_tag="PKJ43_08905"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ72729.1"
                     /translation="DSPPGLLENLCFEAKAANIPEFFPFNADDFLSIKDDFSKTPDYY
                     NRMSYDALSNKYFHRAMKELDEAMKINSNYLETYFSRIALNSMLKQQNSKQDLADKNK
                     AMTLVNDSLSRMPLNENYLALKASLLSNMGKDKEALTQIENALLKFPQSYKLYDLKAS
                     IFRQQNQQEKAIDCYKKMITIDPNKKKSLTTSIAAAYMNLSRPDEALRIINQQIKEQP
                     ESDNSLLFTRAQLLIGMKDFAPAQKDIESILAKDSSNYNYQKLLAQLYFAQGNKRLYF
                     FHTQKALQKLQNTYQNNNEDVEALFEIASIHLSMKQFDEAEATYNQILALFPDNYESL
                     KQKARIKLTQQQWADAISIYDQLEANYKYEEEFLNNKAIAYIQNGNPQKALEYFEKAI
                     ELNPNNKDAIFNRNRLKAEMGNRN"
BASE COUNT          319 a          245 c          335 g          537 t
ORIGIN      
        1 cggttgaggt cactattctt ggttccattt tttgttctgg ttgagtgttt gatattttcg
       61 ttttttgcta atgtctccta ataatatcct gatattgtaa ggttcaaaaa acaaaagagt
      121 tacagtttaa tgtaaccctt tttcattctg ttattttttt atccaggaat ggattgttgt
      181 tacattaatt cctgttgccc atctctgctt ttaagcggtt tcggttgaat atagcgtctt
      241 tgttgttggg gtttaactca atcgctttct caaaatattc gagtgctttc tgtgggttcc
      301 cgttttggat ataagcaatg gctttgttgt tcaaaaattc ttcttcgtat ttgtaatttg
      361 cttccaactg gtcgtaaatc gaaatggcat cggcccattg ctgttgggtt agctttatcc
      421 ttgctttctg tttcaacgat tcgtaattgt cggggaagag ggcaagtatt tgattatatg
      481 tagcttctgc ttcatcgaat tgtttcatcg acaggtgtat cgaggcaatt tcaaaaagag
      541 cttcaacatc ttcgttatta ttttgatatg tgttttgaag tttctgcaat gccttttggg
      601 tgtggaaaaa gtacaaacgt ttgttgcctt gtgcgaagta aagttgcgca aggagctttt
      661 ggtagttgta attacttgaa tcttttgcta gtatcgactc gatgtctttt tgtgccgggg
      721 caaaatcttt cattcctatt aatagttgcg cccgggtaaa caacaacgaa ttgtcggatt
      781 caggttgttc ttttatctgt tggttaataa tccgcagggc ttcgtcgggt cgcgaaagat
      841 tcatgtaggc tgccgcgatc gaggtggtga gggatttctt tttatttggg tcgatggtaa
      901 tcattttctt atagcaatcg attgcttttt cctgttggtt ttgttgcctg aatatcgatg
      961 cttttaaatc gtatagttta tagctttgag ggaatttcaa taaggcattc tcaatttggg
     1021 taagggcttc tttgtcttta cccatgttcg agagcagcga agctttcaac gccaggtaat
     1081 tttcattcaa cggcatgcgt gagagcgagt cgttcaccag cgtcatggct ttattcttat
     1141 cggccaggtc ttgtttcgag ttttgctgtt taagcatcga gttgagggca atccggctga
     1201 aataggtttc caggtagttg ctgttgattt tcatagcttc atccagttcc ttcattgccc
     1261 ggtggaagta tttattactc agcgcgtcat acgacattcg attgtagtaa tcgggtgttt
     1321 ttgagaaatc atctttgatg ctcaggaaat cgtcggcgtt aaaagggaag aattcgggaa
     1381 tgttagcggc tttggcttca aagcaaaggt tttcgagcaa accagggggt gagtcc
//
LOCUS       DAOPUW010000584         1434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_103073_length_1434_cov_2.219724, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000584 DAOPUW010000000
VERSION     DAOPUW010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103073_length_1434_cov_2.219724"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PKJ43_08910"
     CDS             complement(<1..523)
                     /locus_tag="PKJ43_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNZ72730.1"
                     /translation="MKIKATLLLSLLVLGSIFTHAQLSKNPNKFLGNITTMYSVRSDF
                     GDYWNQLTPENETKWASIEGTRDVYNWDTVDKQYQYCKDNNFPFKFHTLIWGGQYPSW
                     MNNLSQKEQLEEVTEWFDAVAARYPDLEYIDVVNEALPGHAPAPFKNALGGDGVSGYD
                     WIVKAFIMARERWP"
     gene            complement(793..1176)
                     /locus_tag="PKJ43_08915"
     CDS             complement(793..1176)
                     /locus_tag="PKJ43_08915"
                     /inference="COORDINATES: protein motif:HMM:NF013522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HNZ72731.1"
                     /translation="MPVVKIELAKGKSKEFKRIFMDAVHESMVETLKIPANDKNIRLM
                     DYDADCFDTKPPYQYFIEIIMFSGRTKETKSKLFRSLVDKLHDKLNIDPQSVFIVINE
                     QPTENWGIRGGISASDVQLDFTIAL"
BASE COUNT          393 a          287 c          277 g          477 t
ORIGIN      
        1 ggggccaacg ttcgcgagcc ataataaatg ctttcacaat ccaatcgtat cccgaaacac
       61 catcgccgcc cagcgcgttt ttgaatggcg caggggcatg tccgggaagt gcttcgttta
      121 caacgtctat atattccaaa tcgggatagc gtgcggcaac cgcatcaaac cattccgtaa
      181 cttcctctaa ttgctctttt tgagagaggt tattcatcca tgaaggatat tgaccgcccc
      241 aaataagcgt atgaaactta aacgggaagt tgttatcctt gcaatactga tattgcttgt
      301 caacggtatc ccaattgtac acatcacggg ttccttctat cgatgcccat tttgtttcat
      361 tctctggtgt gagctgattc cagtaatcgc cgaaatcgct tcttacagag tacattgtag
      421 taatgttgcc caaaaattta tttggatttt ttgacagttg ggcatgagtg aaaatgctgc
      481 ccaacactag caacgataac aaaagtgttg ctttaatttt cataattgtt tggtaattaa
      541 tgattagact tagaatattt ggttgtcata ttaggacgag ggtaatatta caattattca
      601 ttgattttac cttccttttg tattgttaaa ttttggttaa ttggcatatt ttggattaaa
      661 actacttttt ttcagcagtc accgaggttg taaactatta ctcacatgat gatttaaagg
      721 cgttttgcac ttgtttcatt gattaaatat tcaatgaatt caattcatcc tagcgtatgt
      781 tgcctgaccg aattatagcg ctatggtaaa atccaactga acatcggagg ctgaaatccc
      841 accacggata ccccagtttt cggtgggttg ctcgttaata acaatgaaaa ccgactgtgg
      901 atcgatattc aatttgtcgt gaagcttgtc gaccaatgac ctgaatagct tgctttttgt
      961 ctccttggtt ctccccgaaa acatgataat ttcgataaag tactgatatg gcggtttcgt
     1021 gtcaaaacaa tccgcatcgt aatccatcaa ccgaatattt ttgtcattgg ccggaatttt
     1081 tagcgtctca accatagact cgtgaaccgc atccatgaaa atccgcttga attctttcga
     1141 tttccctttg gctaattcaa tttttactac aggcatgttt aatgttttaa atagtgaaaa
     1201 gagctttgtg cgtttatgaa tacggtttct gccgtatctt ttagtttcaa atatatcttt
     1261 cttgtgttga acttacaatc tgcacctgat tttatattca tcagttaagt tttgtgcttt
     1321 tctgaaataa atgtgccggt tctgccatag gtaaaaccgg cacagcacat ggttgtgcaa
     1381 atgaaaaatt cacagcaaac aacccggagg ccttatcctg cgataggagt gagg
//
LOCUS       DAOPUW010000585         1434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_103080_length_1434_cov_2.136331, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000585 DAOPUW010000000
VERSION     DAOPUW010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103080_length_1434_cov_2.136331"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PKJ43_08920"
     CDS             complement(<1..480)
                     /locus_tag="PKJ43_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNZ72732.1"
                     /translation="MIIRVLVWIARYFLALTFIFSGFVKGVDPLGSAYKFSDYFAAFN
                     LGFLEPLALVFAFLLCAAELYIGLLLLFRVKDLIAIWGSFLFMAVFTPLTLVLALYNP
                     VSDCGCFGDAIVLTNWETFFKNIPLLLATMLLFLYRKKLTTVYGLRLNNFLAVFLLAI
                     "
     gene            complement(493..1179)
                     /gene="tmk"
                     /locus_tag="PKJ43_08925"
     CDS             complement(493..1179)
                     /gene="tmk"
                     /locus_tag="PKJ43_08925"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNZ72733.1"
                     /translation="MSFIVIEGLDGAGKSTQLKLLGEYFLHQGKPHESLHFPRTESPF
                     FGELISRFLRGELGKLEEVDPYVVALLFAGDRKDAAGIIHGWIQEGKTVLLDRYVYSN
                     IAFQCAKLASHADQKRLRDWIYQLEFVHFGIPKPDLNIFLDVPFEFTKSKLTQGRDGQ
                     DRNYLQGSNDIHEENLSFQERVRNVYLDQVNYDSSYLIINCTDKHGGMEDQHVIFNKI
                     VQSIAKYLAV"
BASE COUNT          490 a          309 c          269 g          366 t
ORIGIN      
        1 gatggcaagg agaaacactg ccaaaaagtt attcaaacga agtccataaa cggttgttaa
       61 cttctttcgg tagagaaata gcagcattgt agccaacaaa aggggaatat ttttgaaaaa
      121 agtctcccaa ttggtaagta caatggcatc gccaaagcaa ccgcaatcgc tgacaggatt
      181 gtataaagcg agaaccaggg ttaacggagt gaaaacagcc ataaacagga aagaacccca
      241 tatggctatc aaatccttta ctctgaacag caaaagcaaa cctatgtaaa gttcagcagc
      301 gcaaagcaaa aacgcaaaaa ccaaagccaa aggctctaaa aatccaaggt tgaaggcggc
      361 aaaataatct gaaaatttat aggccgatcc caatgggtca acacccttaa caaagccgga
      421 aaaaataaaa gtgagcgcca aaaaatatct tgcaatccaa actaaaactc taataatcat
      481 tgttagaaaa tattaaacgg ctaaatattt tgcgatggat tgtacaattt tgttaaaaat
      541 cacatgctga tcctccattc caccatgctt atcggtacaa ttaattatca aatagctgga
      601 atcataattc acctgatcaa ggtaaacatt acgtaccctt tcctgaaagc tcaaattttc
      661 ctcgtgaata tcgttgctcc cctgcaaata gtttctatcc tgtccatcgc ggccctgggt
      721 cagcttcgat ttagtgaact cgaatggcac atcgaggaag atattcaaat cgggcttggg
      781 tatgccgaaa tgaacaaatt cgagttggta aatccagtcg cggagtctct tttgatctgc
      841 atggcttgcc aacttggcgc actgaaaggc gatgttcgaa tacacatacc tgtcgagtaa
      901 aacggttttt ccttcctgaa tccaaccgtg gataatgcct gcggcatcct ttctatcgcc
      961 ggcaaatagc agtgctacga cgtaggggtc aacctcttcc aacttaccca attcgcctct
     1021 aagaaaacgg gagatcaact cgccaaagaa gggagactca gttctgggaa aatgtagcga
     1081 ttcatggggt ttcccctgat gcaaaaaata ctcgccaagc agcttcagct gggttgactt
     1141 accggcacca tcgagcccct cgataacaat aaaactcata gaaataaaaa attcttgcgc
     1201 tacaaaaata cactttaaac ccgaaagttt tctttaaaaa aaagttaatg acaacttgtt
     1261 ttctgtggaa tttgttttaa cccgctgatt gtaaccaatg tttttcaatt taacattcga
     1321 aaaaccgatt gcctattaaa ttagatgtta ttcaaatgtt tcgtaattta gctacggaat
     1381 caaacgcaga tgcatggcat ggaaaaagta aaatcgtttt ttacctcaac ccat
//
LOCUS       DAOPUW010000586         1432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_103233_length_1432_cov_3.563544, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000586 DAOPUW010000000
VERSION     DAOPUW010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103233_length_1432_cov_3.563544"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PKJ43_08930"
     CDS             <1..662
                     /locus_tag="PKJ43_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ72734.1"
                     /translation="PGENYNGIMSSNEYLTRVNLMGASNDNFDTPVLRGKNVAVIGGG
                     NTAMDSVRTAKRLGAERAMIVYRRSRVEMPARVEEVKHADEEGIEFLNLTNPIEYFAD
                     EKGRVNKMRLQLMELGEPDASGRRSPVEIPGATQDIDVDLVIVAVGVSPNPLIPASLP
                     DLKVSRWGTIEVNDGMQSSIPVLFAGGDIVRGGATVILAMGDGRNAAKSMHEYLSGQK
                     D"
     gene            801..>1432
                     /locus_tag="PKJ43_08935"
     CDS             801..>1432
                     /locus_tag="PKJ43_08935"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNZ72735.1"
                     /translation="MANLTTRFFGLELKTPVIIGSSGLSNSVEKIRLLAQSGAGAIVL
                     KSVFEEEIVNEYKAEFQKQADDQSNLEFLDYFDYEIKNDRVKNYLDLIQGVKKAGIDI
                     PLIASINCASGNEWAFFAKRLAESGADALELNLFLLPSDINRSAEQVHNYYITTINKV
                     LDTVSVPVTVKLSPYFADLARFIKELSETRLAGITLFNRFYNIDIDLNTR"
BASE COUNT          411 a          284 c          343 g          394 t
ORIGIN      
        1 ttcctggcga aaactataac ggcattatgt cgagcaatga ataccttacc cgtgtaaacc
       61 tgatgggagc cagtaacgac aatttcgaca ctcctgtgtt gagggggaaa aacgttgctg
      121 ttattggtgg cgggaatact gccatggact cggtacgtac agccaaacgt cttggtgccg
      181 aacgggccat gattgtgtac cgtcgttcgc gcgtagaaat gcctgcacgt gttgaagagg
      241 tgaaacatgc tgatgaggaa ggtattgaat tccttaacct gaccaacccg atagaatatt
      301 ttgccgatga gaaagggcgt gtaaataaaa tgcgccttca gctgatggaa cttggagaac
      361 ccgatgcttc agggcgtcgc tcgccggttg aaattcccgg agcaactcag gacatcgatg
      421 tcgatttggt aattgttgct gttggggttt ctcctaaccc gcttattccg gctagtttgc
      481 ccgatttgaa agtgagccgt tggggaacca ttgaagtaaa cgatggaatg cagtcgtcaa
      541 ttcccgtgct ttttgcaggg ggcgatatcg ttcgtggcgg cgcaactgta attctggcca
      601 tgggcgatgg aaggaacgca gcaaaatcga tgcacgaata cctgtcaggt caaaaagatt
      661 gattaatttt aatagcgatt aaatagtaaa cacaaagact caaaggcaca aagtataaat
      721 tttttccttt gtgctactta gtgaatgacc ttagtgtcct ttgtggtttc cgatataaaa
      781 aagaaaaacc aaagtatata atggcaaact taacaacccg tttttttgga ttagaattga
      841 aaacccctgt aataataggg agttccggtc tttctaattc tgtggaaaaa ataaggcttc
      901 tggcgcaaag cggtgccgga gctatcgtgt tgaaatcggt tttcgaagaa gaaattgtta
      961 atgaatacaa ggctgaattt caaaaacagg ccgacgatca aagcaatctc gaatttctgg
     1021 attatttcga ttatgaaata aaaaacgatc gggtgaagaa ttatctcgac ctgattcaag
     1081 gggtgaaaaa agcaggaatt gatatcccat tgattgccag cattaactgt gcttcgggca
     1141 acgagtgggc attttttgcc aagcgtcttg ccgaatcggg tgccgacgcc ctcgaactga
     1201 atcttttttt attgccttct gacataaata gaagtgctga gcaggttcat aactattaca
     1261 ttacaactat aaataaagtg ctcgatacgg tgtctgttcc tgtaacagta aagctgtcgc
     1321 cgtactttgc cgaccttgcc cgttttatca aagagctttc cgaaacccgc ctggcaggga
     1381 taaccctttt caatcgattt tataatatcg atattgattt gaataccagg ca
//
LOCUS       DAOPUW010000587         1428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_103708_length_1428_cov_2.871085, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000587 DAOPUW010000000
VERSION     DAOPUW010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103708_length_1428_cov_2.871085"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1428
                     /locus_tag="PKJ43_08940"
     CDS             <1..>1428
                     /locus_tag="PKJ43_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72736.1"
                     /translation="RNDYDYSSITGFATSMEPRNPTLKGRVISDVNTQKGLNKAQVKI
                     IMTFNSRAPYNYTIQTDTTGKFDFTPIFNSICSKNWINDLTYVTIEVSKNGFHYLENG
                     DKKSKYTNGFTPEKFEMGKQVVLDNIHLYADGSIKGRIINENGAPVEAYVQFLENSSS
                     ASAVETGEMHKTSGFYTMGNIALGQTGEYISNGKFQIPAIPGSNRKLVIIPKDVTYFS
                     DTLTINVSGSAVTDLGDIKVYERQHRIYFYVKRTMGGDMYQISQPLPGAKVSVIGAAN
                     TQTFTSDANGKVVMNFKNVSESNLTLKVSGPPGSNYVPKNIAFTNEESETPVQLPTVY
                     LEQGLTVKGKVLLDGNATADAEVYVELSRGIQTDVDINFASDGGQAVSESQYLFTAKP
                     KSDGTFEINTIPPELKNKQVTLKVVYKKSLSSFGRGQTGSTGTQNQLGNNQTITQSHG
                     STDEKTIFGESKQVMIPETSGNLVLN"
BASE COUNT          491 a          305 c          294 g          338 t
ORIGIN      
        1 agaaatgatt acgattactc ctccatcacc gggtttgcca catctatgga accccggaat
       61 cccacattaa aaggaagggt tattagcgat gtgaatactc aaaaagggct aaacaaggca
      121 caggtaaaaa tcatcatgac gttcaattca cgtgccccat ataattacac catacaaacc
      181 gatactaccg gcaagtttga tttcactcca atatttaatt caatttgcag caaaaactgg
      241 ataaatgatt tgacatacgt tacgattgaa gtgagtaaaa atggcttcca ctatctcgaa
      301 aatggcgata aaaaaagcaa atatacaaat gggtttaccc ccgaaaaatt tgaaatgggc
      361 aagcaggttg ttctcgacaa catccatcta tatgctgacg gaagcattaa aggccgcatt
      421 ataaatgaga atggagcacc tgttgaagca tacgtccagt tcctcgaaaa cagcagctcg
      481 gccagcgcag tcgaaacagg cgaaatgcac aaaaccagtg gattttatac catgggaaat
      541 attgctctgg gtcaaacagg ggaatatata tcaaatggaa aatttcaaat tcctgcgatt
      601 cctggcagca accgtaaact tgtgattata cccaaagacg tcacctattt ctccgataca
      661 ttaaccataa atgtatcggg aagtgccgtt accgacctgg gcgacattaa agtgtacgaa
      721 cgacagcatc gtatctattt ctatgtaaaa agaacgatgg gaggcgatat gtaccagatt
      781 tctcaacctt tgccaggagc aaaagtctcg gttattggtg cagcaaacac acaaaccttt
      841 acctccgatg ccaatggcaa agtggttatg aactttaaaa acgtgtcgga aagcaacctc
      901 acgttaaaag tcagtggtcc gccgggaagc aactatgtgc ccaaaaacat tgcatttacc
      961 aacgaagaaa gcgaaactcc ggtacaactg ccaaccgtgt acctcgaaca agggcttacc
     1021 gtgaaaggga aagttctcct tgacggcaat gcgactgccg atgctgaagt atatgttgaa
     1081 ttgagccggg gaattcaaac cgatgttgat ataaattttg ccagcgacgg aggacaggct
     1141 gtttctgaat cgcaatacct ttttaccgca aaacccaaaa gtgatggcac attcgaaatt
     1201 aatactatcc cacctgagct gaagaataaa caggtaacct taaaagtagt ttataaaaaa
     1261 tctttgtcga gttttgggcg gggacaaact ggcagcaccg ggactcaaaa tcaacttggc
     1321 aataatcaaa caattaccca aagtcatggt tcaacagatg aaaaaacaat ttttggtgaa
     1381 tcaaaacagg tgatgattcc tgaaacatcg gggaaccttg tactaaac
//
LOCUS       DAOPUW010000588         1428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_103720_length_1428_cov_2.590677, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000588 DAOPUW010000000
VERSION     DAOPUW010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103720_length_1428_cov_2.590677"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PKJ43_08945"
     CDS             <1..332
                     /locus_tag="PKJ43_08945"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNZ72737.1"
                     /translation="LFLDKGIQVIKQSRGQISANSLAEKLTTTPKSVERKFSNHLGKT
                     PKQYIKLVRFQETLNDFSKHENIHLTEYAYRNGYFDQAHFIRDFKSFSGYTPREFVEK
                     YPDFCVE"
     gene            413..1192
                     /locus_tag="PKJ43_08950"
     CDS             413..1192
                     /locus_tag="PKJ43_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ72738.1"
                     /translation="MAAEIEIRNLFKKYPNGTEALKGVSLEIGRGQFFTLLGPNGAGK
                     STLVKIMTTLISKDSGSFFIAGLNPESNPAKIQHLIGVASQENEIDPTEKVENLLVFQ
                     GRLFGLSKAEANNRADELIELFQLNSERNKKASALSGGNKRRLHCALALVHRPQILFL
                     DEPTVGMDPLARANFWEIITSLNHSQGVTVFLTTQYLDEADKHATEMALIVGGEIHFA
                     GSIAGFKKMVNPDGYLSLDDSYLKYVKSLNNNESLTTNKEE"
     gene            1195..>1428
                     /locus_tag="PKJ43_08955"
     CDS             1195..>1428
                     /locus_tag="PKJ43_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72739.1"
                     /translation="MRTANTILINRGILKLVKQPSAPIGGFAMSVFFLLVYNAGIGGV
                     GNLEAFGTGGYLSFVFPIAVISLAMGSSAGAGQT"
BASE COUNT          413 a          329 c          328 g          358 t
ORIGIN      
        1 aactgtttct cgacaaagga attcaagtga tcaaacaaag ccgcgggcaa atttcagcca
       61 atagtttagc cgaaaaacta accaccaccc ccaaaagtgt agagcgtaag ttttcgaatc
      121 atttgggaaa aaccccgaag caatacatca aactggtgcg atttcaggag accctgaacg
      181 atttcagcaa gcacgaaaac attcatttga ctgaatatgc ctaccgtaac gggtacttcg
      241 accaggcgca ttttatacgc gatttcaaaa gtttttcggg ctacacgccg agggaatttg
      301 ttgagaagta tcccgatttt tgtgtggaat agattttctt ttgtcggttt tttacaattt
      361 ttgctccctc agtcctgcct aattttgctg catcattcaa attgaagcaa ttatggcagc
      421 agaaattgaa attcgaaatc ttttcaaaaa gtatcccaac ggcaccgaag cgctaaaagg
      481 agtgagccta gaaattggcc gcggacagtt tttcaccttg ttgggaccca acggtgctgg
      541 caaatcgacg ctggtaaaaa taatgactac actaatctcg aaagactcgg gaagtttctt
      601 cattgcaggt ttgaatcccg aatcgaatcc tgcaaaaatt cagcacctga ttggcgtagc
      661 ctcgcaggaa aacgaaattg acccaacaga aaaagtagag aacctgcttg tttttcaagg
      721 ccgcttgttt ggtttgtcga aagctgaagc caataatcgg gccgatgaac tgattgaact
      781 tttccagtta aacagcgagc ggaacaaaaa agcctcggca ttatcgggag gaaacaaacg
      841 tcgcctgcat tgcgcattgg ccctggtgca ccgtccgcaa atactttttc tcgacgagcc
      901 gacggttggc atggatcctt tggctcgcgc caatttctgg gaaatcatta cttcgctgaa
      961 ccattcgcag ggcgttaccg tctttcttac aacccagtac ctcgacgagg ccgacaagca
     1021 cgcaaccgaa atggcgttga ttgtaggtgg cgaaatccat tttgccggca gcatcgcggg
     1081 cttcaaaaaa atggtaaacc ccgacggcta cctctcgctc gacgatagtt acctgaaata
     1141 tgtcaaaagc ctgaacaaca atgaatcatt aacaacaaac aaagaagaat agccatgagg
     1201 acagcaaata caatattgat taaccgggga atcctaaaat tggtgaagca accatcggca
     1261 cccataggag gttttgcaat gagtgtgttt ttcctgttgg tttacaatgc cggaattggc
     1321 ggtgtgggca accttgaagc gtttgggaca ggaggatacc tatcgtttgt gtttcccatc
     1381 gccgttattt cactggccat gggctcgtcg gccggtgctg gacaaacc
//
LOCUS       DAOPUW010000589         1427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_103814_length_1427_cov_2.881195, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000589 DAOPUW010000000
VERSION     DAOPUW010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103814_length_1427_cov_2.881195"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PKJ43_08960"
     CDS             <1..388
                     /locus_tag="PKJ43_08960"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNZ72740.1"
                     /translation="SMTREGIDCWVVTGSAQQILLGRLEREFRGSLFRDKMITAHDVS
                     IGKPNPEPYLKAIRKSGYSVSQAIVIENAPMGVESAKAAGLFVVAINTGPLNAQVLKD
                     AGADIVLSGSSELASVWPLIYESLSE"
     gene            complement(396..1103)
                     /locus_tag="PKJ43_08965"
     CDS             complement(396..1103)
                     /locus_tag="PKJ43_08965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HNZ72741.1"
                     /translation="MKYRHLFFDLDNTLYDFDTNAYHAMFEAFTQLGIISELPSFDAF
                     FKVYSGINDELWALYRENKIIKEVLRGLRFERSLGAFGIKPNIPYTSIDDLYLKKMTQ
                     QTALFPETISILSELKKRGYQLHIITNGFKEVQSDKLRNTGLGMLIKNVFISEIIKSP
                     KPQREIFEHSIKSSNARKKESLMIGDSWESDIVGAKNFGIDQVFFNPSQQEIDMAELG
                     EPTFTITCLSQLLEILP"
     gene            complement(1123..>1427)
                     /gene="nadA"
                     /locus_tag="PKJ43_08970"
     CDS             complement(1123..>1427)
                     /gene="nadA"
                     /locus_tag="PKJ43_08970"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNZ72742.1"
                     /translation="KHVGSTAALLSYVQKSNAQKFIVATESGILHQMKKACPSKIFIP
                     APSIDSTCGCNDCNYMKLNSIRKLYLCLKNELPEITLSEEVIEKARKPIERMLSIS"
BASE COUNT          372 a          272 c          335 g          448 t
ORIGIN      
        1 atctatgacc cgcgaaggta tcgattgttg ggtcgttaca ggttcggcac aacagatttt
       61 gcttggccgc cttgaaagag aatttcgggg gtcacttttc cgtgataaaa tgatcacagc
      121 acacgatgtt agcatcggaa aaccaaatcc ggaaccatat ctcaaagcta ttcgtaaatc
      181 tggatattct gttagtcagg ccattgtgat agagaatgca cccatgggcg ttgagtcggc
      241 aaaagctgct ggtctttttg tcgttgccat caatactggt ccgcttaatg cacaggtgtt
      301 gaaagatgcg ggagccgaca ttgttctctc aggaagcagt gaattagcca gtgtctggcc
      361 gttgatctac gaatctttat cggaatagaa tattatcagg gaagtatttc aagcagttgc
      421 gacaagcagg taatggtaaa tgttggttcg cccaactcgg ccatgtcgat ttcttgctga
      481 gatggattga aaaaaacctg atcgatacca aaatttttgg ctccaacaat atcactttcc
      541 cacgagtcgc caatcatgag gctttctttt tttcgggcat tgctgctttt aattgaatgc
      601 tcgaaaattt cgcgctgtgg cttgggcgat ttaataattt ctgaaatgaa aacattcttt
      661 atcagcatgc cgaggccggt gttcctaagt ttatcagatt gaacttcttt aaacccgttg
      721 gtaataatat gaagctggta accccgtttt tttagttcgg aaagtatgga aatggtttcg
      781 gggaaaagcg cggtttgttg ggtcattttt ttcaggtaca ggtcgtcaat gctggtgtac
      841 gggatattgg gttttatgcc gaacgcgccg aggctacgct cgaaacgcaa gccccttaac
      901 acttccttta taattttatt ttcgcggtat agtgcccaaa gttcgtcgtt gatccctgaa
      961 taaactttaa aaaatgcatc gaaagaagga agttccgaaa taatcccaag ttgagtaaaa
     1021 gcttcaaaca ttgcgtgata cgcgttagtg tcgaagtcgt aaagggtatt gtcgaggtcg
     1081 aaaaaaagat gccggtattt caatgtcttg ttttattata tcttacgata tcgaaagcat
     1141 acgttcaatg ggtttgcgtg ctttttctat cacttcttcg gaaagagtaa tttcgggaag
     1201 ttcgtttttc aagcaaagat ataatttcct tatcgagttt aatttcatgt agttacagtc
     1261 attgcatccg caggttgaat ctatcgaagg tgccgggatg aatatcttcg aagggcaggc
     1321 ttttttcatc tggtgcaaga ttccgctttc ggtggctact atgaatttct gagcattgct
     1381 tttttgcaca tagctaagta gtgcagcggt cgatcctacg tgctttg
//
LOCUS       DAOPUW010000590         1425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_104071_length_1425_cov_2.089051, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000590 DAOPUW010000000
VERSION     DAOPUW010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104071_length_1425_cov_2.089051"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PKJ43_08975"
     CDS             complement(<1..391)
                     /locus_tag="PKJ43_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNZ72743.1"
                     /translation="MSDVSEILIRRRSIRKFADEKLSSEETELILRAALLSPTSKNSR
                     SWEFVVIEDKEMLEKLSKCKPNSASFLTGAALAVVVLGNPILSDVWIEDASIASTSML
                     LQAEDLNIGACWIQVRERNYSETITSAD"
     gene            616..>1425
                     /locus_tag="PKJ43_08980"
     CDS             616..>1425
                     /locus_tag="PKJ43_08980"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNZ72744.1"
                     /translation="MKIHEYQAKEIFASYGLPVDKSYVCKNVDEAVKAYREIGSPRAI
                     VKAQVLTGGRGKAGGVKLANNEDELRKHAADILGMTIKGLKVDRILVGQAVNIASEYY
                     VSYIVDRVSKSTILMLSKAGGMDIEEVARTSPEKIHKIIIDPLVGVPDYLAREAAFEL
                     FDRMDQVKQAIPIFQKLYKLFVENDASLAEINPLVLTDEGNVMAIDAKMTFDDNALYR
                     HPQIASLFEPTEDEKKEQNAKSKGFSYVNIGGNIGCMVNGAGLAMATMDIIQ"
BASE COUNT          458 a          296 c          292 g          379 t
ORIGIN      
        1 aatcggcgga agtaattgtt tcggaataat ttcgttcacg aacctgtatc caacatgcac
       61 caatgttgag atcttcggcc tgaagaagca tggaagtaga agcgatggaa gcatcttcaa
      121 tccatacatc actcaatatc gggttaccca aaacgaccac ggccaatgct gcccctgtca
      181 aaaacgaagc tgagttgggt ttgcatttcg acaatttttc gagcatttct ttatcttcaa
      241 tcacaacaaa ctcccatgaa cgactgttct tggaagtagg cgacaacaag gcagctctta
      301 aaattaattc ggtttcttca gaagaaagtt tttcgtcagc aaattttcgg atactccgac
      361 ggcggataag tatttctgaa acgtcactca tgcaataatt tattttcgat tacttatgca
      421 aaagtagcca taaaaatcgc ataaaactta ctaaaccgac aaaatcaaaa gattatcatt
      481 ttttaaacat ttttcagtga tatacgttgt ataacaaaat catgaaattt agaatttctt
      541 caaaagaaga aatatctttg tttaaaaatc gtagtttaaa aatttcatta taataattat
      601 tacattcaaa agcccatgaa aattcatgaa tatcaggcaa aagaaatttt tgcgtcttat
      661 ggacttcctg tagataaaag ttatgtctgc aaaaatgtgg atgaagccgt taaggcctac
      721 agggagattg gttcgcctcg agccattgtc aaggctcaag tactcaccgg gggaagaggc
      781 aaagccggtg gcgtgaaact tgcaaacaac gaagacgagc ttcgcaagca tgcagccgat
      841 attttaggga tgaccatcaa aggattgaaa gtcgatcgta ttctcgttgg tcaggcagtg
      901 aatatcgctt ctgagtatta cgtaagttac atcgtcgatc gggtctcaaa atccacgata
      961 ctcatgctaa gcaaagcagg aggaatggat atagaagaag tggcacgcac ctctcccgaa
     1021 aaaattcaca aaatcattat cgatccactc gtcggtgtac ccgattattt ggcgcgcgaa
     1081 gccgctttcg aacttttcga ccgtatggac caggtgaaac aggctattcc tattttccag
     1141 aaactgtata aattgttcgt agaaaacgat gcctcgcttg ccgaaatcaa tccgcttgtc
     1201 ctcaccgacg aaggcaatgt tatggctatc gatgccaaaa tgaccttcga cgacaatgcg
     1261 ctttatcgcc atcctcaaat cgcatcgctt ttcgaaccga ccgaagacga gaaaaaggaa
     1321 cagaatgcca agagtaaagg atttagctac gtaaacatcg gtggcaatat tgggtgcatg
     1381 gtgaatggcg ccggacttgc aatggccact atggacatca ttcag
//
LOCUS       DAOPUW010000591         1418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_104852_length_1418_cov_3.002935, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000591 DAOPUW010000000
VERSION     DAOPUW010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104852_length_1418_cov_3.002935"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PKJ43_08985"
     CDS             <1..285
                     /locus_tag="PKJ43_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNZ72745.1"
                     /translation="MNTVYGAVFDHQGETPGLGAEIATKSFQQPFEGKQIFENSDQFV
                     SIKVHKGGAKEGDIHGVDAISGGTITSKGLEAMLDSCLILYKPFFIQNRQ"
     gene            301..933
                     /locus_tag="PKJ43_08990"
     CDS             301..933
                     /locus_tag="PKJ43_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HNZ72746.1"
                     /translation="MSIFSSKNVKLLSGPLNLQNPVTVQILGICSALAVTVKLKNAIV
                     MGLSVIVVTAISNLVISMLRNYIPSRIRIIVQLVIVATMVILVDQVLKAFVYDVSKQL
                     SVYVGLIITNCIIMGRLEAFAMGNKPWPSFLDGLGNGAGYALILIIIAFFRELFGSGT
                     LMGFQIMPKAFYEAGYVNNGMMIMPAMALILVGCIIWFQRSYNKELIEKE"
     gene            953..>1418
                     /locus_tag="PKJ43_08995"
     CDS             953..>1418
                     /locus_tag="PKJ43_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HNZ72747.1"
                     /translation="MQEMINIFVKSVFIDNMIFAFFLGMCSFLAVSKTVKTSIGLGAA
                     VIFVQIITVPVNYLLLEYVMKPGALSWLGEGFKDVDLSFLSFMIYIATIAAMTQLVEM
                     VIEKFSPSLYNSLGIFLPLIAVNCAILGGSLFMEQREYENIGQVLSWGLGSGF"
BASE COUNT          405 a          262 c          297 g          454 t
ORIGIN      
        1 atgaataccg tgtatggagc agtatttgat caccaaggag aaactcctgg gttaggcgcc
       61 gaaatcgcta ctaaaagttt tcagcagcct ttcgaaggca aacaaatctt cgaaaattct
      121 gatcaatttg tctccattaa agtacataaa ggcggagcaa aagaaggaga tattcatggt
      181 gttgatgcca tttcgggtgg aacaatcacc tcgaaagggc ttgaagctat gctcgacagc
      241 tgcttgattc tgtacaaacc atttttcatt caaaatcgtc aataattaaa actgattgcg
      301 atgagtattt tttcatcaaa aaatgtaaag cttttgtcag gtccgctgaa cctgcaaaac
      361 ccagttaccg ttcaaatttt aggaatttgt tcggctctgg cagtaaccgt taagttaaaa
      421 aatgccattg ttatggggct ttcggttatt gtggttaccg caatatcaaa ccttgtaatc
      481 tcgatgttga gaaactatat tccttcacgt atacgcatta ttgttcagtt ggttattgtt
      541 gccactatgg taattttagt tgaccaagtg ctgaaagcct tcgtatacga tgtaagcaaa
      601 caactttcgg tatacgttgg attgattatc actaactgta tcattatggg tcgcctcgaa
      661 gcgtttgcta tgggtaacaa accatggcca tcatttctag acggactggg gaacggtgct
      721 ggttatgcct tgattctaat catcattgct ttcttcagag aacttttcgg atcgggaaca
      781 ttaatgggat ttcagattat gccaaaagca ttctatgaag caggttacgt aaataatgga
      841 atgatgatta tgccagccat ggcacttatt ttggttggct gcattatttg gttccaacgc
      901 agttataata aggaactgat cgaaaaagaa taaccactaa aagacaacga ttatgcaaga
      961 aatgattaac atatttgtca agtcggtttt tatcgacaat atgatctttg cctttttcct
     1021 tggcatgtgt tctttcctgg ctgtttctaa aacagtaaag acatcgatag gtttaggagc
     1081 tgcagtcatt tttgttcaga taattaccgt tcctgttaac tacctgctct tagagtatgt
     1141 aatgaaaccc ggagcattat catggcttgg tgaaggcttc aaagatgtag acctttcatt
     1201 cttaagtttt atgatttaca tcgcaaccat tgctgcaatg acacaattag ttgaaatggt
     1261 gatcgaaaag ttctctccat cactttacaa ctcgttgggg atattccttc ctttgattgc
     1321 cgtgaactgt gcaatccttg gaggttcact tttcatggag caacgcgagt acgaaaatat
     1381 cggacaagta ctttcctggg gcttaggttc gggatttg
//
LOCUS       DAOPUW010000592         1417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_104998_length_1417_cov_2.983847, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000592 DAOPUW010000000
VERSION     DAOPUW010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104998_length_1417_cov_2.983847"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /gene="ruvX"
                     /locus_tag="PKJ43_09000"
     CDS             <1..298
                     /gene="ruvX"
                     /locus_tag="PKJ43_09000"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNZ72748.1"
                     /translation="YVAKEQVEAFVVGMPYNFGHQENEVMKFVVQFINELGNKFPSIP
                     IHKVDERFTSKMATAVILQSGVNRKDRQNKATVDKVSASILLQSYMEMRSNKPF"
     gene            348..905
                     /gene="def"
                     /locus_tag="PKJ43_09005"
     CDS             348..905
                     /gene="def"
                     /locus_tag="PKJ43_09005"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNZ72749.1"
                     /translation="MIFPIVAYGDPVLRKKGEEITKDYPELSKLIDDMFDTMHKSRGV
                     GLAAPQIGKAIRLFIIDSRKMYDEGEEEKGLEEVFINAQIIDEEGEEWRFEEGCLSIP
                     TIREDVSRQPKITVQYYDRNFKFQERTFDDLTARVIQHEYDHIEGILFIDHLKPLKKS
                     LLKGKLEKISKGMVDVNYKMKFPKK"
     gene            complement(895..>1417)
                     /locus_tag="PKJ43_09010"
     CDS             complement(895..>1417)
                     /locus_tag="PKJ43_09010"
                     /inference="COORDINATES: protein motif:HMM:NF018648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNZ72750.1"
                     /translation="VAMSGVHATVAGVLLAFFIPLDEENEHSSFQQLTHFLERPVNYF
                     VLPLFAFCNTAIHIQPEYITEIGSPLALGIILGLVFGKTIGIFLFSYLSVKMKWGKLP
                     ERVTWHQIMGMAILGGIGFTMSIFVTLLAFTDNTLIEQSKLYIVIGSCISSIVGIAWL
                     LFTSNQAENKLTS"
BASE COUNT          479 a          250 c          307 g          381 t
ORIGIN      
        1 ttatgtagcc aaagagcagg tggaagcctt tgtagtggga atgccctata acttcggcca
       61 tcaagaaaac gaggtgatga aatttgtggt acagttcatc aacgaattgg gtaacaaatt
      121 ccccagcatt cccattcaca aagtggacga gcgttttact tccaaaatgg ctacagcagt
      181 tatcttacag agcggagtga atcgcaaaga tcgccagaat aaagcaacag tagataaggt
      241 tagcgcttcc atattgttgc aatcgtatat ggaaatgcgc agtaataaac ctttttaagc
      301 aattccaaat aactatattc acacccgtaa aaaagaacga tactgatatg atatttccta
      361 ttgtggctta cggagatcct gttttgagaa agaaaggaga ggagattacg aaagattatc
      421 ccgagttaag caaattgatt gatgatatgt ttgataccat gcacaaatcg cgtggtgtag
      481 gtttggcagc tccgcaaatc ggaaaggcta ttcgcttgtt tattattgat agtcgtaaga
      541 tgtacgatga aggggaagaa gaaaaaggct tggaagaagt atttatcaat gcacaaatta
      601 ttgatgaaga aggagaggag tggagatttg aagaaggttg tttgagtatt ccaactattc
      661 gtgaagatgt tagccgtcag cccaaaatta ctgttcagta ttacgatcgc aatttcaaat
      721 ttcaagagcg tactttcgat gatttaacag cacgtgttat tcaacacgaa tatgatcata
      781 tagaaggcat tttgttcatc gaccacttaa agccattgaa aaagagtttg ctgaaaggta
      841 agctggaaaa gatttccaaa ggtatggtag atgtgaacta caagatgaag tttcctaaga
      901 agtaagcttg ttttctgctt ggtttgatgt aaacaaaagc caagcaatcc ctacgatgga
      961 tgaaatacaa gacccaatta cgatatacag tttcgattgt tcaataagcg tattatcggt
     1021 gaaagccaat aaggttacaa aaatcgacat ggtgaagcct attccgccca atatggccat
     1081 gcccatgatt tgatgccacg ttaccctttc cggcagttta ccccatttca ttttcacact
     1141 taagtaagag aaaagaaaaa taccaatggt tttgccgaat accaatccga gaataatccc
     1201 taatgccaat ggcgaaccaa tttcagtgat atattccggt tgaatgtgaa tggccgtgtt
     1261 gcaaaaagca aagagaggta aaacgaaata attgacaggt cgctccaaga aatgtgtaag
     1321 ttgttggaac gatgaatgtt cgttttcttc atctaaagga ataaaaaatg ccagcaaaac
     1381 gcccgctaca gtagcatgca caccgctcat ggctaca
//
LOCUS       DAOPUW010000593         1407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_106169_length_1407_cov_3.476331, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000593 DAOPUW010000000
VERSION     DAOPUW010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106169_length_1407_cov_3.476331"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="PKJ43_09015"
     CDS             <1..144
                     /locus_tag="PKJ43_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72751.1"
                     /translation="TFGYVTALRTITSGRATSSMEFSHYAEVSNAIAKQVVTEAKGKV
                     ELL"
     gene            274..579
                     /gene="rpsJ"
                     /locus_tag="PKJ43_09020"
     CDS             274..579
                     /gene="rpsJ"
                     /locus_tag="PKJ43_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNZ72752.1"
                     /translation="MSQKIRIKLKSYDHNLVDKSAEKIVKTVKATGAVVSGPIPLPTH
                     KRIFTVLSSTFVNKKSRDQYQLSSYKRLIDIYSSTAKTIDALMKLELPSGVEVEIKV"
     gene            599..1216
                     /gene="rplC"
                     /locus_tag="PKJ43_09025"
     CDS             599..1216
                     /gene="rplC"
                     /locus_tag="PKJ43_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNZ72753.1"
                     /translation="MQGLIGKKIGMTSVFSVEGKNIPCTVIEVGPCVVTQVKTESTDG
                     YDAVQIAFDEKKEKHTTKSELGHFKKASTTPKRKVVEVDPYDVEVTLGQVLTLDLYKD
                     VEWVDITGTTKGKGFQGVVKRHNFRGVNDATHGQHNRLRAPGSLGASSWPSRVMKGMR
                     MGGRDGGERVTVENLQVMKVIPESNLLVVKGSVPGAKGSYLIVWK"
     gene            1216..>1407
                     /gene="rplD"
                     /locus_tag="PKJ43_09030"
     CDS             1216..>1407
                     /gene="rplD"
                     /locus_tag="PKJ43_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNZ72754.1"
                     /translation="MELNVLNIQGKETGKKVVLNDQIFGIEPNDHAIYLDVKQFLANQ
                     RQGTSKSKERGEVSGSRRKL"
BASE COUNT          480 a          253 c          316 g          358 t
ORIGIN      
        1 acattcggtt atgtaacagc tcttcgtacc ataacttcag gtcgtgcaac ttcttctatg
       61 gagttctcgc attatgccga agtatcaaat gctattgcaa aacaggtggt aacagaagct
      121 aaaggaaaag ttgaactttt ataaataata acaatagttt gtgatttcgc tcacaaacta
      181 ttgtttatat gtaaatatgt gaaaaaccgg ttagcgaatg actcttaacc ggcaacaata
      241 ataaatgtac aattaataat taaaaaacag acaatgagtc aaaaaatcag aattaagctg
      301 aaatcgtacg accacaatct ggttgacaag tcggctgaga agattgtgaa gactgtaaaa
      361 gctaccggtg cagttgttag tggtccaata ccacttccta cgcacaagag aatttttact
      421 gttttgagtt caacttttgt gaacaaaaaa tctcgcgatc agtatcagtt aagctcgtac
      481 aagagattga tcgatattta cagctcaaca gcaaaaacca tcgacgcact gatgaaactg
      541 gaattaccca gcggtgtaga ggttgaaatc aaagtttgat aacaaaaatc gtgtagaaat
      601 gcaaggatta attggaaaga aaatcggaat gacatccgta ttcagtgttg aggggaaaaa
      661 tatcccatgc actgtgattg aagttggccc atgcgtggta actcaggtga aaaccgaaag
      721 taccgacggg tacgacgctg tacaaattgc tttcgacgag aaaaaagaaa agcacaccac
      781 aaaatcggag cttggacatt tcaagaaagc aagcacaact ccaaaacgaa aagtagttga
      841 ggtggatcct tatgatgtag aagtaactct tggtcaggta ttaactctcg atctttacaa
      901 agatgtagaa tgggttgata ttaccggcac aaccaaagga aaaggcttcc agggtgtagt
      961 aaaacgccac aatttccgtg gggttaacga tgctacacac gggcagcaca accgtcttcg
     1021 tgccccaggt tcgttgggcg cttcatcgtg gccatcgcgt gttatgaaag gcatgcgcat
     1081 gggcggtcgc gatggtggtg aacgagttac tgttgaaaac cttcaggtta tgaaagtaat
     1141 tcctgaaagt aatttattag tggtgaaagg ttctgtacct ggagctaaag gttcatactt
     1201 aattgtttgg aagtaatgga attaaatgta ttaaacattc agggaaaaga aaccggaaag
     1261 aaagtcgtat taaacgacca gatttttggc atcgaaccga acgatcacgc aatttacctc
     1321 gacgtaaaac agttcttggc caatcaacgt cagggaactt ctaagtcaaa agaacgtggt
     1381 gaagtttcgg gtagtagacg caaactg
//
LOCUS       DAOPUW010000594         1406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_106343_length_1406_cov_2.250925, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000594 DAOPUW010000000
VERSION     DAOPUW010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106343_length_1406_cov_2.250925"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PKJ43_09035"
     CDS             complement(<1..738)
                     /locus_tag="PKJ43_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72755.1"
                     /translation="MNRLRNRLLSLMVIVLLTACNSNKNDSTLTYLVATDTFSESLLV
                     EGTVEAVNSRTLVCPESDEYNIKFLIDDGTLVKAGDTVCILENKQLVDQYENAIKNLE
                     LSKAEYNKIQADLALDLALLEAQVKNNDAQTSITNLDSVQLQYASPTQRKITELELKI
                     AQIQKEKFEHKLSAVKRINQFEIRKAELRIRQNENNVKRWEEQLLQLVLLTPIDGLAM
                     RSISWVTGNKVIEGETAYPPMPLIEIPD"
     gene            complement(1042..>1406)
                     /locus_tag="PKJ43_09040"
     CDS             complement(1042..>1406)
                     /locus_tag="PKJ43_09040"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNZ72756.1"
                     /translation="TKEENKTYKGPKKATQDYRLPTEYEWEYAMSYKDQASITPAKEL
                     HKVNQGDRNKLGLLNLEGNVTEWTISSGSNEKLAPKVVKGSSWKNDSKANQPELVLPN
                     KGTDYIGFRVVCSTIENE"
BASE COUNT          355 a          260 c          278 g          513 t
ORIGIN      
        1 gtcaggaatt tcaattaatg gcatgggagg atatgcggtt tcgccttcta ttactttgtt
       61 tccggttacc catgaaattg aacgcatggc caacccgtcg atgggcgtta aaagcaccaa
      121 ttgcaaaagt tgttcttccc acctttttac gttgttttcg ttttgacgga ttcggagttc
      181 cgcttttctg atctcaaatt ggttgatacg cttaaccgct gaaagtttgt gttcgaactt
      241 ttctttttga atttgagcaa ttttaagttc caattctgta atttttcttt gagtaggaga
      301 ggcgtattgc aattgtactg aatcgagatt ggtaatggag gtttgcgcat cgttgttttt
      361 cacttgtgct tcaagtaagg ccaaatcgag cgccagatcg gcctgtattt tattgtactc
      421 ggcttttgat agctcgagat tttttattgc attttcatac tgatcgacca attgtttgtt
      481 ttcgagtatg caaacggtat cgccagcctt taccagcgtg ccatcatcga tcaaaaattt
      541 gatgttatac tcatcggatt cagggcaaac gagtgttctt gagtttactg cttctacggt
      601 tccttcgacc aataaacttt ctgaaaatgt gtcggttgcg accaagtaag tcaacgtaga
      661 gtcattctta ttgctattgc acgccgttag tagtacaata accataaggg ataaaagtcg
      721 gtttcgtaat ctgttcatct attggttaat tgctcaaaaa acaatgcaat tggaaacttc
      781 atattgtgaa tggactaccc caattgccat ttctatattc ttttgtgggc ataaagttca
      841 aaatgaaaat ttgcaagttg cgttaacaag atgttaagtt agcaaaaacc gtgcaatgat
      901 tgtgttacaa aaaacgaatt cccctcaatt aagggaagtt ttgtgacatt taagtttatt
      961 taactaaaat aatattttct aacgaacttt gtccgatgtg ttgggctgta ttaagcagta
     1021 cagaggtatg ggtcagtgcg attattcgtt ttctatagtc gaacaaacta ccctaaatcc
     1081 aatataatca gttcctttgt ttggcaagac caattccggt tgatttgctt ttgagtcatt
     1141 cttccatgag cttcctttta caacttttgg ggctaatttt tcatttgaac cggaactgat
     1201 tgtccactca gtaacattac cttctaaatt aagcaagcct agtttgtttc tatctccctg
     1261 gtttactttg tgaagctctt ttgcaggagt aatggatgcc tgatccttgt aactcattgc
     1321 gtattcccat tcatattctg ttggaagccg atagtcttga gttgcttttt ttgggccttt
     1381 ataggttttg ttttcttctt ttgtcc
//
LOCUS       DAOPUW010000595         1402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_106812_length_1402_cov_1.625835, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000595 DAOPUW010000000
VERSION     DAOPUW010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106812_length_1402_cov_1.625835"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="PKJ43_09045"
     CDS             complement(<1..1220)
                     /locus_tag="PKJ43_09045"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNZ72757.1"
                     /translation="MKIDFKVSLVLCLMLILCPYLNAQILKGKVYGSVIDKQSHTTLP
                     GVNIVARVDGNNFGTVSDANGNFQFENIPVGRLDMEFSLVGYEKLSMQHVSLTSAKNL
                     VLEAAMTEKVEAVDEIVVKAHKKDELINEFALISARTFTVEESNKYAGSWGDPARMAS
                     NFAGVVTAGDQRNDIIIRGNSPCGLLWRLDGIAIPNPNHFGSMGTTGGPINMLNNNQL
                     ANSDFFTGAYPAEFGNALSGVLDLKMRNGNQHKHEFIGGMGFNGFELGAEGPISKSMH
                     SSYMVNFRYTMMDLMTAMGMFDVGGIPKYADVSFKLFMPTKKMGTFSMIGVGGKSSIT
                     LESQNDSLQGNMTGWTSEMLPGTHVKNRSKMGVLGLSHKYFFSEKSRIESSLSLSYSN
                     SGINVDSIMEPENF"
BASE COUNT          376 a          316 c          280 g          430 t
ORIGIN      
        1 ttgaagtttt caggttccat aatactatca acatttattc ccgaattgga ataactgagc
       61 gataacgaag attcgatgcg gcttttttct gaaaagaaat atttgtgcga cagcccaagt
      121 actcccattt tcgatctgtt tttcacatgc gttccgggta gcatttccga agtccatccg
      181 gtcatgtttc cctggagtga atcgttttgg ctttcgagtg taatagagct tttccctcca
      241 acgccaatca tagaaaaagt gcccatcttt tttgtaggca taaatagttt gaacgaaaca
      301 tcggcatatt ttggaattcc acccacatcg aacatgccca tggctgtcat taaatccatc
      361 atggtgtacc tgaaatttac catgtacgag ctatgcatgc ttttcgaaat tggcccttcg
      421 gcgcccagtt caaaaccgtt aaagcccatt cctccaataa attcatgttt gtgctgattt
      481 ccattgcgca ttttcaggtc gagcactcca gaaagagcat ttccaaactc ggcaggatag
      541 gctcctgtaa agaaatcgga attagccagc tgattgttgt tgagcatatt gatggggccg
      601 ccggttgtcc ccatcgatcc aaaatggtta ggatttggaa tggctatgcc atcgaggcgc
      661 cagagcaaac cacagggtga gttgcctcga atgatgatat cattccgttg atcgccggcc
      721 gtaactacac ccgcaaaatt tgatgccatt cgagctggat cgccccaact tcctgcatat
      781 ttattcgatt cttcaacggt gaaagtacgg gcgctaatga gggcaaattc gttgatgagc
      841 tcgtcctttt tgtgcgcttt cacaacaatt tcatctaccg cttcaacttt ttcggtcatg
      901 gcagcttcaa gtaccaaatt ttttgctgaa gttagcgata catgctgcat ggaaagtttt
      961 tcataaccta ccaaactgaa ttccatgtcg agacgaccaa caggaatgtt ctcaaattga
     1021 aaatttccgt tggcatcgct caccgttcca aagttattgc cgtcaacacg tgcaacaata
     1081 tttaccccgg gtaaggtggt atgcgattgc ttgtcgataa cgcttccgta aaccttccct
     1141 tttagtattt gagcgttaag ataagggcat agaattagca tcaggcatag cacaaggcta
     1201 accttaaaat caattttcat acgataattg tttagatttt agaacttaat aatgttcgtt
     1261 tgtttcatat ttttatccac atcataaagt ccttcatgtt attgtaactc acaagaactt
     1321 cttcgtcgaa gctgggattc agaattattt tcagcctgct tttcgaaaaa gggatcatct
     1381 cctttatggc cgaaacatgg gc
//
LOCUS       DAOPUW010000596         1401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_106904_length_1401_cov_2.057207, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000596 DAOPUW010000000
VERSION     DAOPUW010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106904_length_1401_cov_2.057207"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PKJ43_09050"
     CDS             <1..374
                     /locus_tag="PKJ43_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNZ72758.1"
                     /translation="IWIFGEVKQSVVHVYERHKASGLLTLEKGRVRWFLSLDYDDLPE
                     TVKAKGARTYRHITIENEEIEFSEGFTNLHTETYNRILNNGGFGIDDVRPSILLTDCI
                     RNSEPIGLSGDYHPMLKTISL"
     gene            complement(361..654)
                     /locus_tag="PKJ43_09055"
     CDS             complement(361..654)
                     /locus_tag="PKJ43_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72759.1"
                     /translation="MSKIRELKKDITYLCEQMILDAIQLSDIVNKKDKEKVYSMITDI
                     SVFHNELIARANHPDGKDNPKLVKTYFRNISIELLSGSDKFYDKMNSLLPKEK"
     gene            complement(735..>1401)
                     /locus_tag="PKJ43_09060"
     CDS             complement(735..>1401)
                     /locus_tag="PKJ43_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72760.1"
                     /translation="GSSAFDLNKTLSIEAPSPIELIGQNVFRLMQAVNDSVNEAVEFS
                     IQKAENSSRKYSLLFDVKPDTKYLLEVDSGMIKTYSGQYNAGFSTKFSTQKKDYYGSL
                     VIKMLNAKGPGILQLVKNDKTETIIKELIVSDLTKPLTLGFLNPGTYLIKYIEDLNGN
                     GEWDSGLLAERIHPEAVYYLQKEIIIKSNWEIKEEFEIKRGTIQNKEFAKTTSSSTLN
                     NKK"
BASE COUNT          379 a          272 c          265 g          485 t
ORIGIN      
        1 tgatctggat ttttggcgaa gtgaaacaaa gtgttgttca tgtgtacgaa cggcacaagg
       61 cttcgggatt actgacgctc gaaaaaggaa gagtgcgctg gttcctgagt ctcgattacg
      121 acgacttgcc cgaaacggta aaggcaaaag gagctcgaac ctatcgtcat ataactattg
      181 aaaacgagga gattgaattc agtgaagggt tcactaattt gcacaccgaa acctataatc
      241 gtatattgaa taatggtgga ttcggaattg acgatgtacg gccttcaata ttgttgaccg
      301 attgcatacg aaactccgaa cccattggcc tctcgggcga ttatcaccca atgttgaaaa
      361 ctatttctct ttaggaagaa ggctattcat tttatcatag aacttatcac ttcccgaaag
      421 taattcaata cttatgttcc tgaagtaggt tttcactaat ttgggattat cttttccatc
      481 aggatggttg gctctggcaa ttaactcgtt gtgaaacaca gaaatgtccg taatcatcga
      541 gtagactttt tctttatctt ttttgttaac tatatccgac aactgaatag catcgagtat
      601 catttgttca cacaaatacg taatgtcctt ttttaattca cgaattttcg acatgatatt
      661 tattttaatt gttttgtcat attaatttgc ccagtaatat acccatttga tggcataaaa
      721 catacttgat aagtctattt tttattgttc aaggttgatg agcttgttgt ttttgcaaat
      781 tctttgttct gtatagtgcc ccttttaatc tcgaattctt cttttatttc ccagttcgac
      841 ttaatgataa tctccttttg aagataataa accgcctcag gatgaatcct ttctgcaagt
      901 aacccgctgt cccattctcc gttgccgttc aaatcctcaa tatatttaat caggtaggtt
      961 ccgggattta aaaaaccgag cgtaagtggt ttcgttagat cgctgacaat aagttcttta
     1021 attattgttt ctgtcttatc gttttttacc aactgaagga ttcctggtcc tttagcgttc
     1081 agcattttga ttacgagtga cccgtaataa tcttttttct gggtcgaaaa ttttgtgctg
     1141 aacccggcat tatattgccc tgaataggtt ttaatcatgc ccgaatcgac ctcgagcaag
     1201 tacttggtgt cgggctttac atcgaacagc aatgaatatt ttctactcga attttcggct
     1261 ttttgtatgc tgaattctac ggcctcgttt accgaatcgt ttacggcttg catgagcctg
     1321 aaaacgttct gccctattaa ttctattgga gaaggagctt caatacttag cgttttgttc
     1381 aaatcaaaag ccgatgaacc a
//
LOCUS       DAOPUW010000597         1400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_106957_length_1400_cov_3.627509, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000597 DAOPUW010000000
VERSION     DAOPUW010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106957_length_1400_cov_3.627509"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="PKJ43_09065"
     CDS             <1..1139
                     /locus_tag="PKJ43_09065"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ72761.1"
                     /translation="KNLRSLASQFKAVAIVGPRQSGKTTLAKYVFPDKKYISLENPDT
                     RNFANEDPRGFLDSYNEGAIFDEIQKVPHLFSYLQQIIDETNEPGKFILTGSNNFLLQ
                     EGITQSLAGRIAYQLLLPFSINEIIQSDNVGEMILKGFYPPLYDQKLDAYTWHKNYIS
                     TYVERDVRQLKSINNLETFSRFIRLCAGRTGQLLNLSSLAIEVGVDHKTIGSWLGILE
                     SSYIIHRLQPHYTNFNKRLVKMPKIYFHDTGVLCALLGIQNVEQLNFHPLWGNIFENF
                     VVNELVKKKINFNLRSNLFFWRDSKGYEIDVLVENGLSLYPIEIKSGQTISSEYFKNL
                     NFWKSLTGNEPATVIYNRKETQKRSNGHEVIFWKDIYSTNQIPL"
BASE COUNT          446 a          272 c          279 g          403 t
ORIGIN      
        1 aaaaaaattt acggtcactg gcctcgcaat ttaaggctgt tgccattgtt ggtccccgtc
       61 aatcgggtaa aacaacgttg gccaaatatg ttttccccga taagaaatac ataagcctcg
      121 aaaatcctga cactagaaat tttgcaaacg aagacccaag gggatttttg gatagttata
      181 acgaaggtgc gatcttcgac gaaatccaga aagtgcccca cctgttttct tatctccagc
      241 aaattatcga tgagaccaat gagccgggaa aattcattct tacaggttcc aataattttc
      301 tgttgcaaga aggtattacc caaagtttag cgggaagaat tgcttaccaa ttattgcttc
      361 ccttttcaat aaacgagatt attcaaagtg acaatgttgg tgaaatgata ttaaaaggct
      421 tttatccacc actgtacgac cagaaattag atgcttatac ttggcataag aattatattt
      481 caacctatgt cgaaagagat gttaggcaat taaaaagcat taataacctt gaaacattta
      541 gtcgttttat tcggctatgt gctggaagaa caggtcagtt gctaaattta agcagtcttg
      601 ctatcgaggt tggtgtcgat cataagacca ttggatcgtg gttgggtatc cttgaaagca
      661 gctacatcat tcatcgatta caaccccact acacaaattt caacaaacga ttggtgaaaa
      721 tgccaaaaat atattttcac gatacgggcg ttctttgcgc cttactaggg attcagaatg
      781 ttgaacaact taattttcat ccactttggg gcaatatttt tgaaaacttt gtggtcaacg
      841 aacttgtaaa gaagaaaata aatttcaatt tgcgtagcaa cctgtttttc tggagagaca
      901 gcaagggtta cgaaattgat gtgcttgttg aaaatggatt gtcgctttat cctatcgaaa
      961 taaaatcggg acaaaccatt tcgtctgaat attttaaaaa tctcaacttc tggaaatcat
     1021 taaccgggaa cgaacctgct accgtaattt ataacaggaa agaaactcaa aaacggagca
     1081 atggccatga ggtaatcttc tggaaggata tttattcaac aaatcaaatt ccattataaa
     1141 gttgcttttc aattagagca atggcttttt tttctttccc atgtaacttt ccgtttcagt
     1201 tatggtactt ttaggtgaat caaaccacat aaccgcgtga cgaagcagca acgttttatg
     1261 gatctataca agccggtgag ccgaaaaata tcggcatact gccgggcaat cacccgcagc
     1321 agcgccgagg ccgaggacct tttgcacgat accctgttgg ccgcttacga aaagatggag
     1381 cagttgcgaa gcgacgaagc
//
LOCUS       DAOPUW010000598         1399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_107115_length_1399_cov_2.866071, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000598 DAOPUW010000000
VERSION     DAOPUW010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107115_length_1399_cov_2.866071"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1399)
                     /locus_tag="PKJ43_09070"
     CDS             complement(<1..>1399)
                     /locus_tag="PKJ43_09070"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72762.1"
                     /translation="NLEKNSELKSVLLQESPWLLSAQSESEAKKRIGLLFDLNTMGNE
                     LSRALNRLKQMQTPNGGFPWFSGMPDNEFITLHIVKGMGEMKTMNCVDKKWQNDFDNI
                     LQKAIIYLDKRMEESYNELLIRSKKDSTLLGEYKPSMEEIYYMYARSSYPEIELSENA
                     AKIFQYYYDQVKKNWLTYNEYAQGHIALTANRQNDSSMARRILKSLSDKAIRSKDDGM
                     YWKNNVAGYYWYQAPIETQALLITAFDEMNYDTKAVEEMKIWLLRQKQTTHWKTTKAT
                     LSACYALLSKGYNLLADQKPLSVKLGDKPIEKLRKVTPEAGTGYVKTSFAAAEIAPEF
                     AKISVENQNNGIAWGAAYWQYFEQLDKITAAKTDVQIKKQLFLKMKTEKGSVLRTISE
                     KDPIAVGDEVVVRIEITTRRNMEYVHLKDLRASAFEPVTAISGYKWQDGLGYYSEVKD
                     AAMNFFIGYLPKGSYV"
BASE COUNT          355 a          311 c          258 g          475 t
ORIGIN      
        1 acataacttc ctttaggcag gtagccaatg aaaaagttca tcgcagcatc tttaacttcg
       61 ctgtagtagc ctagcccatc ttgccattta tatcccgaaa ttgcagttac aggctcgaaa
      121 gcagacgccc tgagatcttt taaatgcaca tattccatat tgcgacgggt agtgatttca
      181 attcgcacga ccacctcgtc gccaacggct atcgggtctt tttccgaaat tgtccgtaaa
      241 acactcccct tttctgtttt catttttaag aagagttgtt tctttatttg cacatctgtt
      301 ttggccgcag ttattttgtc gagttgctca aaatattgcc aataagcagc tccccaggca
      361 atgccattgt tctgattttc tacggaaatt ttagcgaatt cgggagctat ttcagccgca
      421 gcaaacgagg ttttcacata tccggttccc gcttctggag taacttttcg cagcttttca
      481 atcggtttat cacccaattt tacagataag ggtttctgat cagcaagcaa attgtatcct
      541 tttgacagca aggcataaca tgcagataac gtggcttttg tagttttcca atgcgtggtt
      601 tgcttttggc gaagaagcca tattttcatt tcttctacag ccttcgtatc ataattcatc
      661 tcgtcgaaag cagtaatgag caacgcttgt gtttcaattg gcgcttgata ccagtaatac
      721 ccggcaacat tatttttcca gtacatacca tcatcctttg atcggatagc cttatctgac
      781 aacgatttca aaatacgtcg tgccatggag ctgtcattct gccgattggc agttaaggca
      841 atatgccctt gtgcatactc attgtacgtg agccaattct tctttacctg atcatagtaa
      901 tattgaaata tttttgctgc attttcagac aattcaattt caggataaga acttcgtgca
      961 tacatataat atatttcttc catggaaggt ttgtattcgc cgagtagcgt tgaatctttt
     1021 tttgaccgaa taagtagctc attatatgac tcttccattc gtttgtcgag atagataatg
     1081 gctttttgaa gaatattgtc gaaatcgttt tgccactttt tatcgacgca attcattgtt
     1141 ttcatctctc ccattccttt cacaatatgc aaggtaatga attcgttgtc aggcattccg
     1201 ctaaaccatg ggaaaccgcc attgggagtt tgcatttgct ttaagcggtt caacgctctc
     1261 gaaagttcgt tacccatcgt gtttaaatcg aacaataagc caatacgttt ttttgcctcc
     1321 gattcgcttt gtgcactcaa taaccatggt gactcctgca acagaacaga tttgagctca
     1381 ctgtttttct ccagatttg
//
LOCUS       DAOPUW010000599         1398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_107309_length_1398_cov_1.346984, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000599 DAOPUW010000000
VERSION     DAOPUW010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107309_length_1398_cov_1.346984"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..601)
                     /locus_tag="PKJ43_09075"
     CDS             complement(179..601)
                     /locus_tag="PKJ43_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72763.1"
                     /translation="MNSLFLFLGIIAFFVVALTFWNALQRRKGNTKEKKSTPPLVDGG
                     CCGMHEVCERDSLIAAFTKDPEYFDDEELDNFAGKDSSDYTEVEVDEFREIFYTLLDE
                     DKPRWVRSLQVRGIQVPDQLKDEILMVVNDMRVQKMHA"
     gene            complement(598..>1398)
                     /locus_tag="PKJ43_09080"
     CDS             complement(598..>1398)
                     /locus_tag="PKJ43_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNZ72764.1"
                     /translation="QFTSALLMIAPTMNNGLTIHLENEVISKPYIQLTLGLMEAYGVK
                     SKWEGNNITIKHQQYVPTSIRVESDWSAASYWYSIVSLMPEAQITLLGLKRNSLQGDS
                     NLHNLFADLGVCTEFVPEGVMLKKKKISTKKFFHDFSNEPDLAQTFAVTCCLLNVPFL
                     FSGVQTLKIKETDRIEALKKELLKLGFVIKESENRLMEWDGERCFPANEPVIETYNDH
                     RMAMSFAPACIPLGSITINDPEVVSKSYPNFWEDLKSAGFKIREIENK"
BASE COUNT          391 a          317 c          278 g          412 t
ORIGIN      
        1 tcctatgccc aatcccccaa acgaggcgtg cgtaatgcct ccgctaatga aaacaagtcg
       61 tcgcgaaacc acgtaaacac cgacgattcc tgttgcaata cttgcgaata ggctacccca
      121 aatcgcattg cgaaaaaagg cataatcgaa aagttcgata atgtccattc aatcaaaatt
      181 atgcatgcat cttttgcacc ctcatgtcgt ttacgaccat gaggatttcg tctttcagct
      241 ggtcgggaac ttgaatgccg cgaacctgta agctgcgaac ccatcgcggt ttatcttcat
      301 cgagaagtgt gtagaatatt tctcggaatt cgtcaacctc gacttcagta tagtccgaag
      361 aatcttttcc tgcaaaattg tcgagttctt catcgtcaaa atattccgga tccttcgtga
      421 aagcggcaat caaactgtca cgctcacaca cttcatgcat gccgcaacag ccaccgtcaa
      481 cgagtggagg agtcgatttt ttttctttcg tgttgccttt tcttctttgt aaagcgttcc
      541 aaaatgtgag cgcaactaca aaaaaagcta taattcccaa aaataaaaac agactgttca
      601 tttattttct atctccctaa ttttaaatcc tgccgatttc aaatcttccc aaaagttggg
      661 ataggatttg gatacgacct cagggtcgtt aatcgtaata gatccgagag gaatgcatgc
      721 cggagcaaat gacattgcca ttcggtgatc attataagtt tctattaccg gttcgttggc
      781 cggaaaacat cgttcgccgt cccattccat cagtcgattt tcggattctt ttataacaaa
      841 accgagtttc aaaagttctt tttttaacgc ctcaatacgg tcggtttcct taattttgag
      901 cgtttgaacc cccgaaaata aaaaaggaac gttcagtaaa caacaagtta ctgcaaatgt
      961 ttgggccaga tcgggttcgt ttgagaaatc gtgaaaaaat ttttttgtcg agattttttt
     1021 ctttttcagc attacgcctt caggcacaaa ttctgtacaa acgcccagat ccgcaaaaag
     1081 attgtgaagg ttcgaatctc cctgcaaact atttctcttc aggccgagca acgttatttg
     1141 ggcttccggc atcagtgaga caatcgaata ccagtatgag gcagccgacc agtcactttc
     1201 gacccgaatt gatgttggaa cgtattgctg atgtttgatc gtgatgttat tgccttccca
     1261 tttcgatttt acaccatagg cttccatcaa tccaagggta agctgaatgt agggcttaga
     1321 aataacttca ttttcgagat gaatggttag cccgttgttc atggttggag ctatcatcaa
     1381 aagcgcagag gtgaattg
//
LOCUS       DAOPUW010000600         1395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_107577_length_1395_cov_2.696269, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000600 DAOPUW010000000
VERSION     DAOPUW010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107577_length_1395_cov_2.696269"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1395
                     /locus_tag="PKJ43_09085"
     CDS             <1..>1395
                     /locus_tag="PKJ43_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNZ72765.1"
                     /translation="FDAFSTFFANNNNTWLFLIFFVGFGIKAGFVPVHSWLPHAHPAA
                     PSHVSGVMSGVIVKLGIYGIFRVITFLESDYLMLGEIILSVSVLTGLYGILNAAVHRD
                     FKRMLAYCTIENIGIIGIGIGLGLIGIGNNSPLLFFLGFSGALLHVLNHSLFKSLLFY
                     SAGSVYQQTHTRDMEKLGGLIKQMPKTAILFLIGAVAIGGMPPFNGFVSEFILYNGLI
                     EGLKTGDITQTSLVILSFAGLSLIGGLSILTFSKTFGVVFLGSPRKKLHTDPQEVSKI
                     MLFPQYIIVAVMLLVAFFPRFFLNNIQYILQDGLKNIAIFSSVASYSDNIVAVNYYFI
                     VLLVVVGIIWGIRSLMVKNREQQIKGTWGCSYVAPNSKMQYTGKSFSKPLGKVFSFLL
                     IEEKKYTELSDGEIFPKKRKYVSHYKDFFEYYIFNFVTQRIVFSANYFKFIQNGRMQS
                     YVLYGILFVVLIFIL"
BASE COUNT          360 a          263 c          296 g          476 t
ORIGIN      
        1 ttcgatgctt tttcaacatt ctttgccaat aataacaaca catggctttt tctgatcttt
       61 ttcgtaggat ttggtataaa agccggcttt gttcctgttc atagctggtt gccccatgcg
      121 catccggcag ctccctcgca cgtgtcgggt gtaatgtcgg gagtaatagt aaaactgggc
      181 atttacggaa tattcagggt cattactttt cttgaatccg attatttgat gcttggtgaa
      241 attattcttt cggtatcagt gcttaccggg ttatatggaa tactgaatgc agctgttcat
      301 cgcgatttta aacggatgtt agcctattgt accatcgaaa atattggcat cattggcatt
      361 gggatcgggc tgggcttgat tggtatcggc aacaactcac cattactttt ctttttagga
      421 ttctcgggcg ccctgttgca tgtgctgaac cattcgcttt tcaaatcgct gctattctac
      481 tcagccggtt cagtgtatca gcaaacccac acccgcgata tggaaaaact gggtgggttg
      541 ataaagcaaa tgcctaaaac agcaatattg ttcctgattg gcgctgttgc catcgggggt
      601 atgcctccct tcaacggatt cgtgtcggaa ttcattttat ataacggcct cattgaaggg
      661 ttaaaaacgg gtgatattac acaaaccagt cttgtaattt tgtcatttgc agggcttagc
      721 ctcattggag ggctttcgat ccttaccttc tcgaaaacat ttggggtggt ttttcttggc
      781 tcaccacgga aaaaattaca tacagatcct caagaagtgt cgaaaattat gctgtttccg
      841 cagtatatta ttgtagctgt aatgcttctt gttgctttct ttccccgctt tttcctgaat
      901 aatattcaat atatacttca agacggcttg aagaatattg ccattttttc gtcagtggcc
      961 agttattcgg acaatatcgt tgctgttaac tattatttta ttgtattgtt ggtcgttgta
     1021 ggaataattt ggggaattcg ttcgctaatg gtgaaaaacc gggaacagca aatcaaagga
     1081 acctggggct gctcctatgt tgcccccaat agcaaaatgc agtataccgg gaaatcgttt
     1141 tcgaaaccgt tgggaaaggt attcagtttt ttactgatag aggaaaagaa atatacagaa
     1201 ctttctgatg gtgaaatatt tccaaagaag cgaaaatatg tttcgcacta taaagacttt
     1261 tttgagtact atatttttaa ctttgttact caacggatag tgtttagtgc caattatttc
     1321 aagtttatac aaaatggccg tatgcaatcg tatgtattat atggaatttt gtttgtggta
     1381 cttatattta tcctc
//
LOCUS       DAOPUW010000601         1391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_108145_length_1391_cov_1.825599, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000601 DAOPUW010000000
VERSION     DAOPUW010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108145_length_1391_cov_1.825599"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..>1391)
                     /gene="frr"
                     /locus_tag="PKJ43_09090"
     CDS             complement(112..>1391)
                     /gene="frr"
                     /locus_tag="PKJ43_09090"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNZ72766.1"
                     /translation="MYLDDSLSHIRAGKASTRLIDGIRVDSYGSMVPISNVAAVSTPD
                     ARSITIKPWDKNMFRAIEKAIIDSDLGIMPENNGEIIRLGIPQPTEERRKQLLKQCKG
                     EGETAKISIRNARRDGIEALKKGLKDGLPEDAEKDAEAKLQKIHDKYIKKVEDMLADK
                     NIDVVYNPLPNHLHVPYSIKAMEAGKHVLCEKPIAMNASEAQLLAETASKYPTLKVME
                     AFMYRFHPQWKKIKELIAEGALGELRTIHSHFSFYNTDPANTRNIPEYGGGALYDIGC
                     YCISFPRFLFDRNPVRVISLIERDQKMKIDCLSSAMLDFGEGKTATFTCSIQLMYGQW
                     CEIMRTKGRIVADIPVNAPNDRPTTIMLTTNSGSVLFTFEAIDQYTLQAEEFANAIIN
                     KSTVPTPLTDAIANLKVIDALFESETNNSWIKL"
BASE COUNT          325 a          285 c          310 g          471 t
ORIGIN      
        1 cggggttaca gtaatttcta tgctgtaacc ctttttgtat tcattcagat agacttttgt
       61 caagtcgttg actgatcatt ttgttataag tggattattt attccctttt ctcataactt
      121 tatccaacta ttatttgttt cactttcaaa aagggcatca attactttca ggtttgcaat
      181 ggcatcagtt aaaggagtcg ggactgtact cttgttaatt atagcatttg caaattcttc
      241 tgcctgaagg gtatattggt caattgcttc gaaagtgaat aacacagatc cgctattggt
      301 tgttaacata atggttgttg gtctgtcgtt tggcgcgttc actgggatat ctgcaacaat
      361 acgccctttt gttctcataa tctcgcacca ttggccgtac atgagttgaa tggagcaagt
      421 gaacgtagcg gtttttcctt cgccaaagtc gagcattgct gagcttagac agtcgatttt
      481 cattttttgg tcgcgctcaa taagcgatat aacccgcact gggtttcggt cgaataagaa
      541 tcgtggaaat gaaatgcagt agcatcctat gtcgtaaagt gcacctccgc catattccgg
      601 aatattacgg gtgttggctg ggtcggtatt gtaaaacgaa aaatgtgaat gtattgttct
      661 aagttcgccc agtgctcctt cggcaatcag ttctttaatt tttttccact gaggatgaaa
      721 ccgatacatg aaagcttcca tcacttttag cgtagggtat ttgctcgctg tttcggccag
      781 taattgggct tcggaagcat tcatcgcgat gggcttttca cagagtacat gtttacctgc
      841 ttccatggct tttattgagt atggaacatg taagtggttt ggtagtgggt tgtaaaccac
      901 atcgatgttt ttgtcggcaa gcatatcttc aaccttcttg atatacttgt cgtggatctt
      961 ctggagttta gcttccgcat ctttttctgc gtcttcgggc agaccgtctt tcaagccttt
     1021 tttcagcgct tcgataccgt cgcgacgtgc gttacgaata cttattttag ctgtttcgcc
     1081 ttcgcctttg cattgtttca gcaactgctt gcgtctttct tctgttggtt gcggaatacc
     1141 caaacggata atctcaccgt tgttttcggg cataataccc aagtcagaat caataatagc
     1201 tttttcaatg gcgcgaaaca tgtttttgtc ccatggtttg atggtgatgg aacgtgcgtc
     1261 gggggtcgat acggcagcca cattgctgat gggtaccata cttccgtaag agtctacacg
     1321 gataccgtca ataagtctgg tactagcttt tccggcacgg atatgcgaaa gagaatcatc
     1381 cagatacata a
//
LOCUS       DAOPUW010000602         1388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_108467_length_1388_cov_2.348837, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000602 DAOPUW010000000
VERSION     DAOPUW010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108467_length_1388_cov_2.348837"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PKJ43_09095"
     CDS             <1..591
                     /locus_tag="PKJ43_09095"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72767.1"
                     /translation="LWLKQVPNTIETTDLPEQMVFHLDGKGDWIWGNLFLLQHRNWPE
                     GESERDRNFAEMDCYFWKDDAQEWKGQRANNWFVEVSVPTWRHIICTYDNVTSEFHGY
                     IDGVHITHFDGAEYGGVKRWQAGGEEVPLGDLKFNNPEKFAIGAWCDRLAGTDLQNDD
                     WASPFKGLIDEFRIYDRALTAAEAKDLYDAEVTQIN"
     gene            658..>1388
                     /locus_tag="PKJ43_09100"
     CDS             658..>1388
                     /locus_tag="PKJ43_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72768.1"
                     /translation="MRVNPLLLVLLLFFFSCKKNETDGVVAEILIDSVTVNGLGMVNG
                     GTVHEIDYNAQDIKIYFANEVDSSLFNPAKLYFTGPSNTAFKYSFQKNKKVLCITPQQ
                     KLEPVSIYKIIFDEGENLGGINKIFYIFKFHTRIDSTYKFPQIEDEELLTLVQRQTFK
                     YFWDYAHPVSGLARERFGSGDVVTSGGSGFGMMAILVGISRNFITRSEGFERLNTIVN
                     FLNNPETDRFQGAFPHWLNGSTGKV"
BASE COUNT          395 a          269 c          327 g          397 t
ORIGIN      
        1 ttgtggctaa agcaggtacc caataccatt gaaacaaccg accttcctga acaaatggtt
       61 ttccatttag atgggaaggg cgattggatt tggggtaacc tattcttatt gcagcatagg
      121 aactggcctg aaggtgaatc agaaagagac cgtaacttcg ccgaaatgga ctgctatttt
      181 tggaaagatg atgctcaaga gtggaaagga cagcgtgcaa acaattggtt tgttgaagtt
      241 tctgtaccaa catggaggca tattatctgc acatacgaca atgttacttc cgaattccat
      301 ggctatattg atggagttca catcactcat ttcgacggag cagagtacgg tggtgttaaa
      361 cgttggcagg ctggtggcga ggaagtacct ttgggtgacc taaaatttaa taaccccgaa
      421 aaatttgcca ttggggcatg gtgcgatcgt cttgcaggta ctgatttaca gaatgatgac
      481 tgggcatccc cattcaaggg cttaatcgat gagttccgta tttacgacag ggctctcaca
      541 gcagccgagg caaaagatct atacgatgcc gaggttacac aaataaacta acattctttt
      601 tttaatgagg ggaggcgctt ttcttcccct cattttttcc tccttaaatc ttttgtgatg
      661 agggtaaatc ctttattgct ggtgcttttg ttgttttttt tctcgtgtaa aaaaaacgaa
      721 acagatgggg tggttgcgga aattcttatt gactccgtaa ccgtcaatgg gctgggcatg
      781 gtcaatgggg gaactgttca cgagatagac tacaatgctc aggatataaa gatttacttt
      841 gccaatgagg tcgatagctc cctgttcaat ccggcaaaat tgtactttac cggaccctcc
      901 aacactgctt ttaagtattc ctttcagaaa aacaaaaagg ttttatgcat taccccgcaa
      961 cagaagttag aacctgtttc catctataag ataatttttg acgagggtga aaaccttgga
     1021 ggaataaaca aaatattcta tatttttaaa ttccataccc ggattgacag cacatacaag
     1081 ttcccccaaa ttgaagatga ggaactgctt acccttgttc aacggcagac ctttaaatac
     1141 ttttgggatt acgctcatcc cgtatcgggg ttggcaaggg aaaggtttgg gtcgggtgat
     1201 gttgtaacat cgggcggttc aggatttggc atgatggcta tcctggtggg aatatcgagg
     1261 aacttcatca ctcgaagtga aggttttgaa aggctaaaca ccattgtaaa ttttttgaac
     1321 aaccccgaaa ccgataggtt tcaaggagca tttccgcact ggctcaacgg gagtacagga
     1381 aaagttta
//
LOCUS       DAOPUW010000603         1382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_109192_length_1382_cov_2.993218, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000603 DAOPUW010000000
VERSION     DAOPUW010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109192_length_1382_cov_2.993218"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PKJ43_09105"
     CDS             complement(<1..832)
                     /locus_tag="PKJ43_09105"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HNZ72769.1"
                     /translation="MKILVLNCGSSSIKFQMFDMTDQTVLSKGVVEKIGLNGSFLKLV
                     KQNGDEVIFEGEILDHKIGIEYVLGVLSSKKHGCVANLNEISAVGHRVVHGGEKFNSS
                     VLITDEVVAKMEECIDLAPLHNPPNLKGIEAIKEILPNMIQVGVFDTAFHQTMPKYAY
                     MYGIPYSLYQKYGIRRYGFHGTSHRYVSRKACSLLGIDYNCSKIITCHLGNGASIAAI
                     KNGVSVDTSMGFTPVEGLLMGTRAGDLDIGVVTYLMEKEKLGVETASTLFNKHSGMLG
                     L"
     gene            complement(921..>1382)
                     /locus_tag="PKJ43_09110"
     CDS             complement(921..>1382)
                     /locus_tag="PKJ43_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNZ72770.1"
                     /translation="SIHFMINSVDSRIVEITKGLHTSDEAFEKVSHFIKMVKRKPIPV
                     EESAGRISVRMYSIMLNEACQLVLEGAGNIDDIEQLMMTGYGMRLGPFALADIMGLER
                     TVRWMENLFNEFGEARYKPNPLIKKMIRQHRLGLDCGKGFFFYDENGERVK"
BASE COUNT          395 a          314 c          243 g          430 t
ORIGIN      
        1 tcagtccgag cattccgcta tgtttattaa acaatgtgga agcagtttca acgcccaatt
       61 tttctttttc cataagataa gtaaccacgc caatatctag gtcaccggca cgtgtcccca
      121 tcaacaaacc ctcgacaggg gtgaacccca tcgatgtatc aacagaaacg ccatttttaa
      181 ttgcagcaat agaagccccg ttcccaagat ggcaagtaat gattttcgaa cagttgtaat
      241 caattccaag cagtgaacat gcttttcggc ttacataccg gtgacttgtt ccatgaaaac
      301 cataccttct gatcccgtat ttctggtata agctataggg aataccatac atgtaggcat
      361 attttggcat tgtttgatgg aatgctgtgt cgaaaacccc aacttgtatc atgttgggaa
      421 gaatttcttt gatagcttca attcctttaa ggtttggcgg attgtgaagt ggtgcaaggt
      481 cgatgcattc ttccattttt gccacaactt catcggtaat taaaacacta ctgttgaatt
      541 tttcacctcc gtgaacaacc ctgtggccta cagccgatat ctcgttaaga ttggcaacac
      601 atccatgctt cttgctcgag agcactccca gaacatattc aataccaatt ttatgatcca
      661 gaatctcgcc ttcaaaaatt acttcatcac cgttttgctt gaccaatttg agaaatgaac
      721 cattcagtcc aattttttca acaacgcctt tggaaagcac cgtttggtcg gtcatgtcaa
      781 acatctgaaa tttgatggat gaactaccac aatttaatac taatatcttc atttgaatac
      841 ttttcaattt tagttattta ggtttcacct gttggatttg tgaaaacccg aattaattca
      901 ctgaaaaatt aaggaaacga ttatttaacc cgttcaccgt tctcgtcgta aaaaaagaac
      961 ccttttccac agtcgagccc caagcgatgt tgtctaatca tcttttttat cagcggattt
     1021 ggtttatatc gagcttcgcc gaattcgttg aagaggtttt ccatccaacg cacggtacgt
     1081 tcgagcccca taatatcggc aagagcaaaa ggtccaaggc gcatcccata accagtcatc
     1141 ataagttgtt caatatcatc aatattcccg gcaccttcga ggactaactg acaagcttcg
     1201 tttagcataa ttgaatacat tctaacgctt attcttccag ccgattcttc tacaggaatt
     1261 ggtttgcgtt tcaccatttt aataaagtgg cttacttttt caaaggcttc atccgaagtg
     1321 tgcagtccct tggtgatttc aacaatacgt gaatcaaccg aatttatcat gaagtgaatg
     1381 ct
//
LOCUS       DAOPUW010000604         1381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_109314_length_1381_cov_3.071644, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000604 DAOPUW010000000
VERSION     DAOPUW010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109314_length_1381_cov_3.071644"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..>1381
                     /gene="topA"
                     /locus_tag="PKJ43_09115"
     CDS             169..>1381
                     /gene="topA"
                     /locus_tag="PKJ43_09115"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNZ72771.1"
                     /translation="MVENLVIVESPAKAKTIEKFLGKDYLVKSSFGHIRDLDKKGFGV
                     DIDNNFKPNYIIPDDKKNVVKELKSLAHDASTVWLASDEDREGEAIAWHLKEVLELDP
                     KNTKRIVFHEITKNAILDAINSPRPLDVNLVDAQQARRVLDRLVGFEASPVLWKKVKP
                     QLSAGRVQSVAVRLIVDREREINNFKITSAFRVTAVFLVEDAGKTVELKAELPKRFDT
                     REEAYAFLEKCKNATFTVEDVSTKPTKRSPAPPFTTSTLQQEASRKLGFSVSQTMSIA
                     QKLYEAGKITYMRTDSVNLSSLAINTAKAKVEELFGDKYSKTRQYKTSSKGAQEAHEA
                     IRPTYLDKESVSGTKQEQRLYELIWKRTIASQMSDALLEKTTISILISSANEKFVATG
                     EVIVFDGFLKVY"
BASE COUNT          427 a          263 c          292 g          399 t
ORIGIN      
        1 gagttgtaaa taaattttga acatagcttt caattttctt taatttgcaa attattttta
       61 aagctgtctt ttttctggtc tgttttataa taatttacac cagttaaaaa ataggatggt
      121 ttatccggct tgtaaggtcg gttcaatttt caggaataaa attggtctat ggtagagaat
      181 ttggtaatcg tagagtcccc cgctaaagca aaaacgattg aaaagtttct ggggaaagat
      241 tatttggtta aatcaagttt tggccacata agagatttgg ataaaaaggg gtttggtgtt
      301 gacatcgaca ataatttcaa gccaaactac attatacccg atgataaaaa gaacgtagta
      361 aaagaactca aatcacttgc tcatgatgct tcaacagtat ggctcgcttc cgatgaggac
      421 cgtgaaggag aagccattgc ttggcatttg aaagaagtac ttgaacttga cccgaaaaac
      481 acaaaacgaa ttgtttttca cgaaattacc aagaatgcta ttctcgacgc aattaactct
      541 ccccgtccac tggatgttaa tttggtcgat gcacagcagg ctcgccgtgt gcttgatcgc
      601 cttgtggggt tcgaagcttc gccggttcta tggaaaaaag taaaaccaca actttcggct
      661 ggccgggtac aatcggttgc tgtccgtctt attgttgatc gcgaacgtga aatcaacaat
      721 ttcaagataa cttcagcatt cagggttacc gccgtttttc ttgttgaaga tgccgggaaa
      781 actgttgaac tgaaagctga acttcccaaa cgctttgata ctcgtgaaga agcgtatgct
      841 tttctcgaga aatgcaaaaa tgcaaccttc acggttgaag atgtttcaac gaaaccaacg
      901 aaaagatcac cggcaccacc atttactacg tcgactttgc agcaggaagc cagccgaaaa
      961 ctcggttttt cggtttcgca aaccatgtcg attgctcaaa aattatacga agccgggaaa
     1021 attacctata tgcgtactga ctcggtaaac ctttcttcgt tagctataaa tactgcaaaa
     1081 gcaaaagttg aagagctttt tggtgataaa tattcaaaaa cccgtcaata taaaacgagt
     1141 tcaaaaggtg cacaggaagc gcacgaagcc attcgtccta cttacttgga taaggagagt
     1201 gtttcgggca cgaaacagga gcaacgctta tacgagttga tttggaagcg tactattgcc
     1261 tcacagatga gcgatgccct tcttgagaaa acaactattt ctattcttat ttcttcagca
     1321 aacgagaagt ttgttgcaac cggggaagta attgttttcg atggattttt gaaggtatac
     1381 t
//
LOCUS       DAOPUW010000605         1377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_109807_length_1377_cov_3.356278, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000605 DAOPUW010000000
VERSION     DAOPUW010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109807_length_1377_cov_3.356278"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PKJ43_09120"
     CDS             complement(<1..743)
                     /locus_tag="PKJ43_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72772.1"
                     /translation="MKTQILSLFLILTIVLQSNAQTAESGTELKKSPFQFSIFIPPIG
                     TNGMYFYNTINDFSFNTFIGAGAATNFCELNGFMGFDRYYADGLQASGFASVVGFGAD
                     AQEYSSEGVQLSGFLNANGNSYSGVQMAGFANVNNRFQGLQVSGFLNANRNAAKAMMY
                     AGFLNVNNIVDKTVELAGFANVSFKGASIAQLSGFGNVAQDVKGIQGSGFFNVARDIQ
                     GVQGAGFINVARKVRGVQASGFINICDSI"
     gene            complement(870..1142)
                     /gene="rpmA"
                     /locus_tag="PKJ43_09125"
     CDS             complement(870..1142)
                     /gene="rpmA"
                     /locus_tag="PKJ43_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNZ72773.1"
                     /translation="MAHKKGVGSSKNGRESESKRLGVKIWGGQDAKAGNILVRQRGTQ
                     HNPGENVGIGRDHTLFALVDGTVLFKRKAKNRSYVSVVPFAAEEQN"
     gene            complement(1161..>1377)
                     /gene="rplU"
                     /locus_tag="PKJ43_09130"
     CDS             complement(1161..>1377)
                     /gene="rplU"
                     /locus_tag="PKJ43_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNZ72774.1"
                     /translation="FDIEKVLLIDNDGAVKVGTPTVEGAKVTAKVLEHDRGETLIVFK
                     KKRRKGYKKKNGHRQYLTRIQIEEIVG"
BASE COUNT          425 a          323 c          238 g          391 t
ORIGIN      
        1 tcaatactgt cgcaaatatt aatgaaaccc gatgcctgca ccccgcgcac tttgcgtgca
       61 acattgatga agccagctcc ctgtacacct tgaatatcgc gagcaacatt aaagaacccc
      121 gatccctgaa tccctttcac atcttgagcc acgttgccaa aacccgaaag ttgtgcaatg
      181 cttgcacctt taaatgaaac gttggcaaag cctgccaatt caacagtttt gtcaacaata
      241 ttgttaacat ttaaaaaacc ggcatacatc attgctttag cggcattccg gttagcattc
      301 aaaaaaccac taacctgaag tccctgaaaa cgattgttga cattggcaaa accggccatt
      361 tgaaccccgg agtagctgtt tccattggca ttcaaaaaac ccgataactg aacaccttca
      421 gagctatatt cctgagcatc ggcaccaaaa cccactacag aagcaaaccc cgaagcttga
      481 agcccatcgg cataataccg atcgaaaccc ataaacccgt tcaattcaca aaaattagtg
      541 gcagcaccgg caccgataaa cgtattaaaa gagaaatcgt tgattgtatt gtaaaaatac
      601 attccgttag tgccaatagg cggaataaaa attgaaaact gaaaggggct ttttttaagt
      661 tctgtacccg attcagctgt ctgtgcgttc gactgtagga caatagtcaa gatgagaaat
      721 aaagataaga tttgtgtttt catggctgta aaaatttatt tacagtaacg acagaagaat
      781 atcacagggg ttgcaaaaac gaaaacatac caacaaaaaa agccttccaa atttggaagg
      841 ctttctgaat atcttaataa atatcgatat tagttctgtt cttcagcagc aaaaggaacc
      901 accgaaacgt acgatctgtt tttagctttt cttttgaaaa gaacagtacc gtcgaccagt
      961 gcaaacaaag tatggtcacg accaatacct acattttcgc ccggattgtg ctgtgtgcca
     1021 cgttgacgaa caagtatatt gcctgcttta gcatcttgtc caccccaaat tttaacgcct
     1081 aatcgtttac tttccgattc gcgtccgttc ttcgaactac ctacaccttt cttatgagcc
     1141 atggtatcta atttttttcg ttatcctaca atttcttcaa tttgaatacg ggtcaagtat
     1201 tggcgatggc catttttctt cttataccct tttctacgtt ttttcttgaa aacgataagt
     1261 gtttcgcctc tgtcgtgctc taaaacctta gcagtaactt tagctccttc aacggttggg
     1321 gttccaactt tcacagcgcc atcattgtca atcaaaagta ctttttcgat atcaaaa
//
LOCUS       DAOPUW010000606         1366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_111151_length_1366_cov_2.495042, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000606 DAOPUW010000000
VERSION     DAOPUW010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111151_length_1366_cov_2.495042"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..764)
                     /locus_tag="PKJ43_09135"
     CDS             complement(174..764)
                     /locus_tag="PKJ43_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNZ72775.1"
                     /translation="MARITDINRVKRLKDSTMKLVVENGFGGASAAMIAIDAKVASGY
                     FYMHYKGKYEMVNSLLQEVFQEAAYKLDEYLKTEHSFIQIVEEIVEHIFLIAEKDPVK
                     LKFLYVLSNDYSFVIDDEIRLNVFNFLKRLKELGQESNSIDPEISEEDLYLLLLVNSI
                     QYINLRFKFFSAKGELTHADKEHLLYLIQKVLIQNS"
     gene            1058..>1366
                     /locus_tag="PKJ43_09140"
     CDS             1058..>1366
                     /locus_tag="PKJ43_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNZ72776.1"
                     /translation="MSKYIGAHVSASGGVENAPINAHEIGATAFALFTKNQRQWVAKP
                     LTEESIAAFKLNCEKFGYKPSQILPHDSYLINLGHPEAEPLQKSREAFLDEMHRCEQL
                     G"
BASE COUNT          412 a          254 c          252 g          448 t
ORIGIN      
        1 gtcggcttct ttctcaagta caagatgttg tgtttgagaa agtgcaaggt tgttgctttt
       61 catcagttgt aacgcttcgt cgaatgtaag cacgctgttt tgtgccattg ttgccgatga
      121 cattgccagc atgatgaaaa gaggaccaat aaatgatttt tgttttttca tatttaacta
      181 ttctgtatca agactttctg aattaaataa agtaaatgtt ccttgtcggc atgagttaac
      241 tcgccttttg ccgaaaagaa cttaaaacgc aggttgatgt attgtattga gttaactaat
      301 agcaataagt agaggtcttc ttcactaatt tcagggtcga tagaatttga ctcttgccct
      361 agctctttta accgtttcag aaaattgaaa acgttgagcc tgatctcatc gtcaataaca
      421 aaactgtagt cgttcgacaa tacgtataag aatttcagct taaccggatc tttttcagca
      481 atcaagaaaa tgtgttcgac aatttcctca acaatctgaa taaaagagtg ttctgtcttg
      541 aggtattcgt cgagtttata tgcagcttcc tgaaaaactt cctgaagcag cgaattcacc
      601 atttcgtatt ttcctttgta gtgcatgtag aagtagcccg atgcgacttt tgcatcgata
      661 gcgatcattg ctgccgatgc acccccaaaa ccattttcaa caaccagttt cattgttgag
      721 tctttgagcc ttttaacccg atttatatct gtaattcgtg ccatttaatt aatgaatgaa
      781 tgttcattta tttaatgaca caaacataaa accttttcta tccttttgtg ttgggcataa
      841 tatgaattaa aaattaaatt ttggtttcga tattaattga caataaatca gtgtatttaa
      901 tgtggttact ccgttaagta atgtttaagt cggaataaaa ttcgactaaa atgagctcgg
      961 aaattcatcc aatgtttcca tttcgaaagg cttattgggg catagcttca acctttttta
     1021 atttatcttt gttgtaataa ctcaaaaaaa caagattatg tcgaaatata ttggagctca
     1081 tgttagtgca tcgggcggag ttgaaaatgc cccaataaac gcacacgaaa taggtgcaac
     1141 agcatttgca ctttttacaa aaaatcaacg tcaatgggtg gcaaaacccc ttaccgaaga
     1201 gagcattgcc gcatttaaat tgaattgcga aaagtttggt tacaagccat ctcaaatact
     1261 tcctcacgat agttatctca ttaaccttgg gcatcccgag gccgaaccgc ttcaaaaaag
     1321 ccgcgaagct tttctcgatg agatgcatcg ttgcgaacag cttggt
//
LOCUS       DAOPUW010000607         1363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_111535_length_1363_cov_2.652905, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000607 DAOPUW010000000
VERSION     DAOPUW010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111535_length_1363_cov_2.652905"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /gene="rsxC"
                     /locus_tag="PKJ43_09145"
     CDS             complement(<1..1203)
                     /gene="rsxC"
                     /locus_tag="PKJ43_09145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNZ72777.1"
                     /translation="MLKTFKIGGVHPAENKISAGKPIKDLPIPNEVKIMVSQHIGAPS
                     KALVKKGDEVKVGQLIAQSAGFVSTNIHSPVSGKIVKIEQITDTSGFKKEAIIIETNG
                     DEWDESIDRSDALIHETTLDGKAIIEKISSAGIVGLGGATFPTHVKLLPPPGAKADIL
                     IINGVECEPYLTADHRLMLEKGDEILIGTRLLMKALNVDKACIGIENNKPDAIENLTQ
                     KASKHQGISVVPLKVKYPQGGEKQLIKAITGREVPSGALPINVGAVVNNVGTAFAVYE
                     AVMKNKPLIERVVSVTGKTIKEPSNYRVRIGTPVNNLIEAAGGIPENAGKIISGGPMM
                     GKAVTSTDIPVTKGTSGILIMDEKESHRQETENCIRCGRCVSACPMGLEPFLMMPLVE
                     INDWERCEK"
BASE COUNT          304 a          295 c          293 g          471 t
ORIGIN      
        1 tttttcgcaa cgctcccagt cgttaatttc aactaaaggc atcataagga atggttcgag
       61 acccattgga catgcactta cacatctgcc acagcggata cagttttctg tttcctggcg
      121 gtggctttct ttttcatcca tgatgagtat ccctgatgta cctttagtta ccggaatgtc
      181 ggtggaagta accgctttgc ccatcattgg accaccactg attattttac ctgcgttttc
      241 gggtattccg cctgctgctt caattaaatt gttgacagga gtacctatgc gaacgcgata
      301 atttgagggc tctttaattg ttttgcctgt tacgcttaca actctttcaa taaggggctt
      361 atttttcatt actgcttcgt atacggcaaa tgctgttccc acgttgttta ctaccgcacc
      421 tacgttgatg ggaagggctc cagatgggac ttcacggcca gtaatggctt taatgagctg
      481 tttttcacca ccctgagggt atttcacctt tagtggaaca accgatattc cttggtgttt
      541 tgaagccttt tgggttaaat tttctatggc atcgggcttg ttattttcaa taccaatgca
      601 agctttgtct acgttcaatg ctttcatcaa caggcgtgtg cctatcagaa tttcgtctcc
      661 tttttcgagc ataagccgat ggtcggcagt gagataaggc tcacattcaa caccatttat
      721 aataagaata tcagccttgg ctccaggagg cggaagcagt tttacatgag tagggaaggt
      781 ggcgccccct aatccaacga ttcctgccga ggaaattttt tcaataatgg ctttgccgtc
      841 gagcgttgtt tcgtggatta atgcatctga ccggtctata ctctcatccc attcatctcc
      901 attggtttcg ataatgatgg cttctttctt aaatcctgat gtatcggtaa tttgttctat
      961 cttaactatt tttcccgaga cgggtgaatg gatattggtt gagacaaaac cggcgctttg
     1021 agcaattaat tgtcccactt ttacctcatc gccctttttt accaaggcct tggatggggc
     1081 accaatgtgt tgcgatacca tgatttttac ctcgttgggt attggcaggt ctttgatggg
     1141 ttttccagcc gaaattttgt tttctgccgg atggactcct ccaattttaa atgttttcaa
     1201 cacgacggat ataattttac gttcgtaacg agtttgttta ctttgtttta tgcatcaact
     1261 ttttcggttg tggcattttc agttatcttt tcttttctcg ggggaaagtt ggtctcgata
     1321 atggaattgg ttgggcatac agcaacacat ttgcggcaca att
//
LOCUS       DAOPUW010000608         1362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_111650_length_1362_cov_2.780413, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000608 DAOPUW010000000
VERSION     DAOPUW010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111650_length_1362_cov_2.780413"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PKJ43_09150"
     CDS             <1..362
                     /locus_tag="PKJ43_09150"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HNZ72778.1"
                     /translation="NEFIVLDTVHADGKLSLGENIADLGGLNIAYTAFKKGSNQKEPI
                     EGFTPDQRFFLAYAHLWASNVRDKEILRLTQEDVHSLGRFRVLGPLRNMPEFHTAFDI
                     KDGDYMYLSEDQRAVIW"
     gene            complement(438..941)
                     /locus_tag="PKJ43_09155"
     CDS             complement(438..941)
                     /locus_tag="PKJ43_09155"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNZ72779.1"
                     /translation="MGLDVSQAEGIKTSWQRSILLVLSITLHNIPEGLAVGVAFGALA
                     NNPDVGMLSGAIALAFGIGLQNFPEGAAVSVPLRREGFSRFKAFTYGQLSGVVEPIAG
                     VLGAYLVLTVQPLLPYALSFAAGAMIFVVVEELIPESQTGNDTHFSTIGALLGFAVMM
                     VLDVALG"
     gene            complement(878..1231)
                     /locus_tag="PKJ43_09160"
     CDS             complement(878..1231)
                     /locus_tag="PKJ43_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72780.1"
                     /translation="MEKLLTINPVLLALFATLFTWGLTALGSGMVFFFKTINTKVLNM
                     MLGFAAGVMIAASFWSLLSPAIDMAESNGQIGWVPALVGFSYLVSTSYCHTFTWGSTF
                     HRLKGSKLHGSEVFC"
BASE COUNT          457 a          325 c          261 g          319 t
ORIGIN      
        1 acaatgaatt cattgttctg gataccgttc acgccgatgg caaactttct ctcggtgaaa
       61 atattgccga tttaggcggg ttaaacattg cctacacagc ctttaaaaaa ggaagcaatc
      121 aaaaggaacc gatcgaaggt tttactcccg atcaacgatt ctttctggct tatgctcatt
      181 tatgggccag caatgtacgt gacaaagaaa ttctgcgcct aacccaggaa gacgttcatt
      241 cgttaggccg tttcagagta ctggggccac ttcgcaatat gcccgaattc catacagcat
      301 tcgatataaa agatggagac tatatgtatc tatcagaaga tcaacgagct gttatctggt
      361 aaataaataa tcgattttaa aaaaagcagg tgtcggaaat atcaggcacc tgcttttttt
      421 tgattggttg agaattgtta tccaagggca acgtcaagaa ccatcatcac ggcaaaacca
      481 agtagcgcac caatggttga aaagtgagta tcgttccccg tttgagactc agggataagt
      541 tcttccacta caacaaaaat catggcaccg gccgcaaaag acaaagcata agggagcaaa
      601 ggctgaacag tcaagactaa ataagctccc aaaaccccgg cgataggttc aactacaccc
      661 gacaattgcc cgtaggtaaa tgctttaaat cgcgaaaaac cttcgcgacg caatggcacc
      721 gacaccgcag ctccttccgg aaaattctgt aacccaatac caaaagccag ggcaatagct
      781 cccgaaagca tacccacatc ggggttattg gccaatgcac caaaagctac cccaacagcc
      841 aagccctcgg gaatattatg caaggtaatc gacaacacta gcaaaatact tcgctgccat
      901 gaagttttga tcccttcagc ctgtgaaacg tcgagcccca tgtgaaggtg tggcaataac
      961 ttgtcgacac caaataagaa aagcccacca gagcaggtac ccagccaatt tgcccgttgc
     1021 tttcagccat atcgattgcg gggcttaaaa gtgaccaaaa actcgccgca atcattacac
     1081 cagcggcaaa cccaagcatc atattcagca ccttggtatt aatcgtcttg aagaagaaga
     1141 ccatacccga tcccaacgct gtcagccccc atgtaaaaag cgtggcaaat aacgccagca
     1201 atacagggtt gattgtcaat agcttttcca tatatctctt tcatttttaa tttcgaccaa
     1261 gctaatttag aataattaaa aatagcatca aattttaact tccaataatg ataaaaaaat
     1321 aactctaaaa gtataaaagt aaaatataca taatatcagc ag
//
LOCUS       DAOPUW010000609         1361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_111811_length_1361_cov_2.559724, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000609 DAOPUW010000000
VERSION     DAOPUW010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111811_length_1361_cov_2.559724"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PKJ43_09165"
     CDS             <1..760
                     /locus_tag="PKJ43_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNZ72781.1"
                     /translation="TEYVKRQQIGAKGLVYVKYGSDGSLKSSVDKFYDEADLKKWADA
                     CGAKPGDLLLILSGEKAKTQIQMGALRLEMARQLNLMDKNVFCPLWVVDFPLLEWDED
                     TSRWHAMHHPFTSPKPEDIAMLDTEPGKVRANAYDLVINGVEIGGGSVRIFDSGLQAK
                     MFGLLGFTPEQAQYQFGFLMNAFKYGAPPHAGIAFGFDRLVSMFAGLDSIRDVIAFPK
                     NNTGRDVMMDAPSTIANEQLDELNLKVEIKEVKK"
     gene            955..>1361
                     /locus_tag="PKJ43_09170"
     CDS             955..>1361
                     /locus_tag="PKJ43_09170"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNZ72782.1"
                     /translation="MRTITTILVLTIFFSSGQFLLAQEKAASQCEFAALPYAYNALEP
                     YIDAQTMEIHYSRHHKGYFDNFMKAIKGTELEGKKLSEIFATISQQPISVRNMGGGYY
                     NHNLFWENLSPTPGKLSKELQTELEKQFGSVDA"
BASE COUNT          428 a          283 c          288 g          362 t
ORIGIN      
        1 tactgaatac gtgaaacgcc agcaaattgg agcaaaagga ttggtatatg tgaaatatgg
       61 ttccgatggc agcctgaaat cctcggtcga taaattctat gacgaagccg acctgaagaa
      121 atgggccgat gcctgtggtg caaaaccagg tgatctgtta cttattcttt caggagaaaa
      181 agcaaaaact caaatacaga tgggggcact tcgcctcgaa atggctcgcc agttgaacct
      241 catggataaa aacgtattct gtccgttgtg ggtagtcgat tttcctttat tggagtggga
      301 tgaagatacc agccgctggc acgccatgca ccatccgttt acctcaccaa agccagaaga
      361 catagccatg ctcgacaccg agccaggtaa agtccgtgcc aatgcgtacg acctcgttat
      421 aaacggtgtg gaaattggtg gaggctcagt acgtattttc gacagtgggt tacaggctaa
      481 aatgttcggg ttactcggat ttactcccga gcaagctcaa tatcagtttg gcttcctgat
      541 gaatgctttc aaatatggtg cacctcccca tgcaggtatc gctttcgggt tcgaccgttt
      601 ggtgagcatg tttgccggct tggattcgat ccgcgacgtg attgccttcc cgaaaaacaa
      661 taccggtcgc gatgtgatga tggatgcccc ttcgacaatc gccaacgagc agttggatga
      721 actgaacctg aaagttgaga ttaaagaagt gaaaaaataa atagtcagta acgacttttt
      781 gaaaaaagct tgccggagta ttattcccgg caagcttttc tttttaataa tatacaaatt
      841 aacacattac acaaataagg tataatcaaa taacgacgag ttcacgttca tatataaacg
      901 attcaacttt ttagcttttc ctttgtttat taagaaaaca ataaaaaagt aattatgaga
      961 acaataacaa ccatcttagt attaacaata ttcttttcat ctggtcaatt tttattggca
     1021 caagaaaaag cagcctcaca atgcgaattt gcagccttgc cctacgcata taatgcgtta
     1081 gaaccctata ttgatgcaca aaccatggaa attcattaca gccgtcatca caaagggtat
     1141 ttcgacaact ttatgaaagc gataaaagga acagaacttg aaggaaagaa gctttctgaa
     1201 atcttcgcca cgattagtca acaacctata tctgtgcgca atatgggcgg tggatattac
     1261 aatcacaacc tgttctggga aaacctgtct cctactccag gaaaactttc aaaggaatta
     1321 caaactgaac ttgaaaaaca gtttggatcg gtagacgcat t
//
LOCUS       DAOPUW010000610         1360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_111908_length_1360_cov_3.088123, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000610 DAOPUW010000000
VERSION     DAOPUW010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111908_length_1360_cov_3.088123"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..>1360)
                     /locus_tag="PKJ43_09175"
     CDS             complement(196..>1360)
                     /locus_tag="PKJ43_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNZ72783.1"
                     /translation="NTAFMQVYLPKSVLLMEKIKRFSMSRYSSADKCYLLPATPEVFK
                     ALLVHYEPDNLTLNNCLPPNYLKKENLPNRKRFLLEKAKHQLLEKVPEAGQEFMTNMI
                     DNMLANNLSDSTISNYGNAFFRFIRDHDYTDPAKMDYKQIVRYLGGLMEKGLSSAVGN
                     NLVNALNYYYRNVEQNNEFMFKLPRPKKEKRIRTVFTMEECYQVFQTIENPKHKLALM
                     IAYGAGLRVSEVVNMKWKNVFFKEHKIHIINGKGKKDRIVMLPLSLLEMLEHYQALYR
                     KGKYVFEGQITGMPYSSSSVQKVMREAIEQSGMTKKGSIHSLRHSFATHLLDSGIDIR
                     YVQELLGHKDIRTTMIYSHLSKPAVNNVQSPLDRIMDSTESDEDESDAKKKRH"
BASE COUNT          329 a          318 c          255 g          458 t
ORIGIN      
        1 ttcaactctg cccgctatgt tgtattttta cgcttgccgg taactagtaa atatcgtcaa
       61 ttttggcctg ttttttaggt ttattttgtt aaacagatat tgccgatttt taactctttc
      121 ttcatccttt tcttcattct tttctcggtt tgctatcacg tttaaaaata tacatattct
      181 gtataataac aaatatcaat gccttttttt cttcgcatca ctttcatctt catcgctttc
      241 tgtcgaatcc attatcctgt ccaaagggct ttgtacgtta tttactgccg gcttgctcaa
      301 atgcgaataa atcatcgtgg tgcgtatatc tttgtgtccc agcaactcct gtacataccg
      361 tatgtcaatc cccgaatcga gcaaatgcgt ggcaaaactg tgccgcaaac tgtgtatgct
      421 gcctttcttc gtcatgccac tttgctctat ggcttctcgc atcacttttt gaacactgct
      481 gctactatag ggcatacccg taatttgtcc ttcgaaaaca tattttccct ttcggtacaa
      541 cgcttggtaa tgttccagca tttccaataa cgataaaggc aacatcacaa tcctgtcttt
      601 cttgcctttg ccattaataa tatggatttt atgttccttg aaaaacacat tcttccattt
      661 catattcacc acttcgctta ccctcaggcc tgcaccatat gcaatcatca aggccagttt
      721 gtgtttgggg ttctcaatgg tttggaacac ctggtagcac tcttccatgg taaacaccgt
      781 gcgaatgcgc ttttcctttt tcgggcgggg caacttaaac ataaattcgt tgttctgttc
      841 cacattccgg taatagtaat tcagtgcgtt tacaaggtta ttgcccactg ccgacgataa
      901 acccttctcc attaaaccgc ccaaataacg aactatctgc ttgtaatcca tctttgccgg
      961 gtcggtgtaa tcgtgatcgc gtataaaacg gaaaaaagca ttgccgtaat tactgatggt
     1021 cgaatcgctc aggttgttgg ccagcatgtt atctatcatg ttggtcatga attcttgccc
     1081 ggcttcaggt actttctcga gcagctggtg ttttgctttt tcaagcagaa aacgtttccg
     1141 gttgggcagg ttctcctttt ttaggtaatt aggtggcaaa caattattta aagtcaggtt
     1201 gtcgggctcg tagtggacca gcaaagcctt gaaaacctcg ggggttgccg gcaacaaata
     1261 gcatttatct gccgaactgt agcgcgacat agaaaaacgc ttgatctttt ccatgagcaa
     1321 aaccgacttt ggcagataaa cctgcatgaa cgcagtattg
//
LOCUS       DAOPUW010000611         1356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_112489_length_1356_cov_2.619523, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000611 DAOPUW010000000
VERSION     DAOPUW010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112489_length_1356_cov_2.619523"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..869
                     /gene="trpC"
                     /locus_tag="PKJ43_09180"
     CDS             87..869
                     /gene="trpC"
                     /locus_tag="PKJ43_09180"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNZ72784.1"
                     /translation="MTILDQINADKRLEVAAAKAAVSIAELMKYPAYNKPVPSFSEFL
                     LNPEKSGIIAEHKRQSPSKGIINGNVNLAQVVKGYEEGGASAVSVLTDQKYFGGNLAD
                     LKEATSLLSIPVLRKDFIVDEYQIHEAKAYGAAIILLIAASLTAEEVDRFAKLARELG
                     MEVLFEVHNEEELLKVSDYVNVVGVNNRDLKTFHVDIQQSVKLSALIPGKFIKISESG
                     ISDPETVKMLKTHGYQGFLMGENFMKEANPGEAFRNFVQKIK"
     gene            880..1326
                     /locus_tag="PKJ43_09185"
     CDS             880..1326
                     /locus_tag="PKJ43_09185"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNZ72785.1"
                     /translation="MNVANIRKLLTDKGIEAEIIEHDRPIHSKNDAAGIFKIEETAPT
                     LILDTDRGFLALIISGARERVDFKQLKKSMNCKKLQLANQAEIAEKFNITAGQVPLIG
                     HNFPCILDEKLFQFPFVYGGTGDLYHTLKISPQQLECANDVILKIE"
BASE COUNT          454 a          262 c          299 g          341 t
ORIGIN      
        1 tcgtaagcac aactacattt gaaggtggaa ttgaagccgt gatgatggaa aagaccacta
       61 aaaaataata aatcgtaaat aataaaatga ccatattaga tcaaataaac gcagataaac
      121 gcttagaagt tgcagcagca aaagcggctg tttcaattgc tgagttgatg aagtatcctg
      181 cttataataa accggttcct tcgttttcag aatttttgct gaaccccgaa aagagtggta
      241 tcattgccga acacaaacgg cagtcgccat cgaaagggat catcaacggg aatgtgaacc
      301 ttgctcaagt ggtaaaaggg tacgaagaag gcggtgcatc ggctgtttcg gtgcttaccg
      361 accagaaata tttcggaggt aacctggccg atctaaaaga agctactagt ctgctttcaa
      421 tcccggtttt gcgaaaagat ttcattgtcg atgagtacca gattcacgaa gccaaggctt
      481 acggagcagc tattatccta ttgattgcag cttcgttgac ggccgaagag gttgaccgtt
      541 ttgccaagct tgcaagggaa ctgggtatgg aagtcttgtt cgaagttcac aacgaagagg
      601 aactactgaa agtgtcggac tacgtgaatg tggtgggtgt aaacaaccgc gacctgaaga
      661 cctttcatgt tgatattcaa cagtcggtga aactttcggc gttaattcct ggaaaattca
      721 ttaagatttc agaaagcgga atcagcgatc ctgaaacagt gaaaatgctg aaaactcatg
      781 gttatcaagg cttcctgatg ggtgaaaact tcatgaaaga agccaatccc ggtgaagctt
      841 tccgtaactt cgtgcaaaaa ataaaataga ggaactacaa tgaacgtcgc caacatacgc
      901 aagttattaa cagacaaagg aatcgaggct gaaattattg aacatgaccg gccaatccat
      961 tcaaaaaacg atgccgcggg tatctttaaa attgaagaga ctgccccaac tttaatcctc
     1021 gataccgaca gaggttttct tgcgctaatt atttcaggag caagagaaag agttgatttc
     1081 aagcaattga aaaaatcaat gaattgcaaa aaattgcaac tggctaatca agctgaaatt
     1141 gcagaaaaat tcaacatcac agcaggacaa gttccactga ttgggcacaa ttttccctgt
     1201 attttggatg aaaagttgtt ccagtttcca ttcgtttacg gaggaaccgg agatttgtat
     1261 cacacgttga aaattagccc tcaacagtta gaatgcgcaa acgacgtaat tttaaaaatt
     1321 gaatgaccat gattcactac gaaaacatag tcccct
//
LOCUS       DAOPUW010000612         1354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_112730_length_1354_cov_3.407236, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000612 DAOPUW010000000
VERSION     DAOPUW010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112730_length_1354_cov_3.407236"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PKJ43_09190"
     CDS             <1..650
                     /locus_tag="PKJ43_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNZ72786.1"
                     /translation="VDDLLLMLDYGIFYEFIDMDNFFVVNPLVIPLWKVEKGINYAMV
                     ISTNGGLWRYVIGDTVSFTSTNPYKIKITGRTRHFINAFGEELIIDNAEKGLKKACET
                     TGATINEYTAGPVFMGDDQKGCHQWIIEFEKQPENVEEFAEVLDNELKLLNSDYEAKR
                     YKNLTLRSPQIIIAPQGTFYLWLKNKGKVGGQNKIPRLSNNREYLDSLLEILPKS"
     gene            774..>1354
                     /locus_tag="PKJ43_09195"
     CDS             774..>1354
                     /locus_tag="PKJ43_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008630468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNZ72787.1"
                     /translation="MHIAVAGNIGAGKTTLTTLLAKHYNWEPNYETVDNNPYLDDFYN
                     DMQRWSFNLQIFFLNTRFKQIVEIRKSEKTIIQDRTIFEDAEIFAPNLHDMGLMSTRD
                     FENYRALYNLMIALIQPPDLLIYLRASVPKLVSQIQQRGRAYEESIRLDYLKMLNERY
                     ELWISRYEGSKLLIINVDDTDFQNNPEDLSKII"
BASE COUNT          459 a          295 c          251 g          349 t
ORIGIN      
        1 gtgttgacga tttgctactc atgctcgact atggaatttt ctatgagttt atcgacatgg
       61 ataacttctt tgttgtaaac ccattagtaa tacctctttg gaaagtcgaa aaaggaatta
      121 actatgctat ggtaatttcg accaacggcg gtttatggcg atatgttatt ggcgacactg
      181 taagtttcac atcgacaaac ccatacaaaa taaaaattac tggacgtact cggcacttta
      241 tcaatgcttt tggagaagaa ctcattattg acaatgcaga aaaggggctg aaaaaagctt
      301 gcgaaacaac cggtgccacc atcaacgaat acactgcagg ccccgttttc atgggagacg
      361 atcagaaagg atgccatcaa tggattatcg aatttgaaaa acaacccgaa aacgtggaag
      421 aatttgccga agtactcgac aacgaactga aactcctcaa ctcagattac gaagcaaaac
      481 gatacaaaaa ccttacactc agatcgccac aaataattat tgccccccag ggaacgttct
      541 acctttggct taaaaacaaa ggaaaagttg gcgggcaaaa caaaattcca aggctttcaa
      601 ataaccggga atacctcgat tcgttactcg aaattttacc aaaatcataa atcacaggaa
      661 tattttacac ccttcttctg tcgacaaaaa ttcccgaata ttttagaaaa aataggtcgt
      721 gttagagcac gaattattac cttcgcatga agtaaaatta aatctcgaat actatgcaca
      781 ttgctgttgc cggaaatatt ggggcaggaa aaaccacact taccacattg ctagccaagc
      841 attacaattg ggaacccaac tacgaaacag tcgataacaa tccgtatctc gacgattttt
      901 acaatgatat gcaacggtgg tcattcaacc ttcagatttt ttttctgaat acccgcttca
      961 aacaaattgt agaaatacga aaatcggaaa agacgattat tcaagaccgg accatctttg
     1021 aagatgccga aatttttgcc ccaaacctgc acgacatggg cctaatgtcg acgcgcgatt
     1081 ttgaaaatta ccgtgcactc tataacctca tgattgccct tattcagcca cccgatttac
     1141 tgatttacct gagggcttca gtcccaaaac tggttagcca gattcagcaa cgtggccggg
     1201 cctacgaaga atcgataagg ctcgattacc tgaaaatgct taacgagcgc tatgaacttt
     1261 ggattagccg ttacgaaggc agtaaactct taataatcaa cgtagacgat actgattttc
     1321 aaaataatcc tgaagacctg agtaaaatca tcga
//
LOCUS       DAOPUW010000613         1354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_112751_length_1354_cov_2.803695, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000613 DAOPUW010000000
VERSION     DAOPUW010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112751_length_1354_cov_2.803695"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1354
                     /locus_tag="PKJ43_09200"
     CDS             <1..>1354
                     /locus_tag="PKJ43_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72788.1"
                     /translation="VQITEINVSATNKGTTKNEMVYLSGRSFNVEETERYAGTNGDPA
                     RMASYFAGVMPSGDTRNDIIIRGNSPLGVLWRLEGVDIPNPNHFAAAGTNGGPICILN
                     NNQLSNSDFITSAFPASYGNALSGVFDLKMRNGNNQKRENTIQFGTNGLEAGIEGYFS
                     KSSKASYMINYRYAFLELFDKIGIDLGIPTIPKYQDISFKLSFPSKKGMVSLWGLGGK
                     SSMEMFSDKNEMSTIRNINTFFGSKMGATGLSHSVSFNPTSYIKTSLAVTGSNSYMRA
                     DSVLSASSPYTIYGSDFSEVRAIITSTYHLKVNPKLRITSGITYTHEFVDLIDTAKNE
                     NTFLTVTNINDNFGFLQSHAQGEYAINNKLKFNAGLHMQYLMFNEKFSVEPRAAIKYE
                     VSPKLNFRIAYGLHSQKQQNIVYFFRSLTDTLNLAYKETNKNLDFTKSHHLALGTGWY
                     L"
BASE COUNT          425 a          280 c          271 g          378 t
ORIGIN      
        1 gttcaaatta ccgagatcaa tgtaagtgca accaacaaag gaactaccaa aaatgaaatg
       61 gtttatttaa gcggacgtag ttttaatgtt gaagaaaccg aacgctatgc cggaacaaac
      121 ggcgaccctg ctcgtatggc aagctatttt gcaggagtga tgccttcggg cgatacaagg
      181 aacgatatta ttatccgcgg aaattcaccg ttgggggttt tatggcggtt agaaggggtt
      241 gacataccca atcctaacca ttttgccgca gcaggtacca acggaggccc tatttgcatt
      301 ttaaataata accaattgtc aaattccgat tttataacca gtgctttccc tgccagctat
      361 gggaatgccc tgtcgggggt ttttgatctg aaaatgcgga atggcaataa tcaaaaaaga
      421 gaaaatacga tacagttcgg cacaaacggc ctggaagcag ggattgaagg atatttttcc
      481 aaatcttcaa aagcttcgta catgatcaat taccgctatg cctttctgga gcttttcgat
      541 aaaattggca tcgaccttgg tattccaacg attccaaagt accaggatat ttcctttaaa
      601 cttagctttc cttcaaaaaa ggggatggtt tccttgtggg gtttgggtgg taagagcagc
      661 atggaaatgt tctccgataa aaatgaaatg agcaccatca ggaatatcaa caccttcttt
      721 ggttcaaaaa tgggggctac cgggctttcg cacagcgtca gctttaatcc tacctcttat
      781 attaaaacct cactggctgt taccgggtcg aattcgtata tgagggctga ttctgtttta
      841 tccgcatcat ccccttatac tatttatgga agtgacttca gcgaagtccg cgctataatt
      901 acatcaacat accatttgaa agtaaacccc aaattacgga taacatcagg cataacttat
      961 acccatgaat ttgttgattt aattgacacg gcaaaaaatg aaaatacatt tttaaccgtc
     1021 accaatatca atgataattt cggatttttg cagtcccatg cccaggggga atatgcaatc
     1081 aataacaaat taaagttcaa cgctggtcta cacatgcagt acctgatgtt caacgaaaaa
     1141 ttttcagttg agcccagggc tgcaataaaa tatgaagtaa gccccaagct taattttcgg
     1201 atagcttacg ggcttcattc gcaaaaacag caaaacattg tttatttttt caggtcgctc
     1261 accgatacac ttaacctggc ttataaagaa acaaacaaga atcttgactt taccaaaagc
     1321 catcatcttg cactaggaac aggttggtat ttaa
//
LOCUS       DAOPUW010000614         1344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_114103_length_1344_cov_3.474011, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000614 DAOPUW010000000
VERSION     DAOPUW010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114103_length_1344_cov_3.474011"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..738)
                     /locus_tag="PKJ43_09205"
     CDS             complement(220..738)
                     /locus_tag="PKJ43_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72789.1"
                     /translation="MKKVILLSLLFIAAVAVKAQEPVFVKGDKAVNVGIGFDNYLNLS
                     LSGEMGIMDGIADKGTIGAGVYAGAGTYLLHSSWYANHYRAMAGVRGTFHYPIIEKLD
                     TYAGIGVGLEYWSYKYNSSYWGYDDYSYNGVDLDAGFFIGARYPLTQKFTVFGELGYG
                     IGYLTAGISFKF"
     gene            complement(934..>1344)
                     /locus_tag="PKJ43_09210"
     CDS             complement(934..>1344)
                     /locus_tag="PKJ43_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNZ72790.1"
                     /translation="HELNEMEHLFDRIKNYNVTVKEIDNKVIFLRKLVRGGSNHSFGI
                     HVARMAGMPKSLIKRSEEILSQLEDTHRKDSLSKPLESIGNEREGLQLSFFQLDDPVL
                     KQIRDEIANLDINNLTPIEALNKLNEIKKIAGLK"
BASE COUNT          411 a          267 c          256 g          410 t
ORIGIN      
        1 gttttttcgt gaaaaataaa ttgcaataaa acaccacatt gtgttagttc ttaaacaaca
       61 caagttaaaa acgtttctaa aatgaaaaga aaaaaggttg tcccaaaagg taaataagtg
      121 gtttcaaaga aagattttaa ggaattttat tccctcagaa actacttaat ggaagtaggg
      181 aactcacaaa tccttttgag acaaccggag aaatattttt tagaatttga aagaaatacc
      241 agcggttaag tagccaatac cgtaaccaag ctctccaaaa acagtgaatt tttgggtaag
      301 aggatagcgg gcaccaataa agaacccagc atcgagatca acgccgttat aagaataatc
      361 gtcatatccc caataacttg aattatactt gtatgaccaa tattcaagtc ccacaccaat
      421 acctgcatag gtgtcaagct tttcaataat tgggtaatgg aatgttcccc gaactcccgc
      481 catagcgcga taatggttag cgtaccaact tgaatgaagc aaataagtgc cagctcctgc
      541 atacacacca gctccaattg ttcctttatc tgcaattccg tccattattc ccatttcacc
      601 agataaggaa aggttaagat agttgtcgaa acctatacca acattaacgg ctttatcgcc
      661 ctttacaaaa acgggttcct gtgctttcac tgccacggca gcgataaaaa gaagagagag
      721 tagaattact tttttcattg cgaacaaatt tagttttcga ttatgattat atgttgaaac
      781 aaatttatga cattgtaaac aaatttcaac gggtattttg aatctgtagg gaataaactt
      841 tgaagtgtag ttgtctggaa agaattgaaa cacgggaatc attaaacgac agcttttgtt
      901 tcgacggatt tattcgccct tttcctgaaa agcttatttc aacccagcga ttttctttat
      961 ctcgttgagt ttattcaacg cctcgatggg agtaaggttg tttatatcga ggttggcaat
     1021 ttcgtccctg atctgtttaa gcaccggatc gtcgagctgg aagaagctaa gttgtagtcc
     1081 ttcacgttcg tttccaatgc tttccagtgg tttcgacagg gaatctttcc gatgggtatc
     1141 ttccagttgc gataaaatct cttcggatct tttaatgagg cttttgggca tgccggccat
     1201 tcgtgccacg tggataccaa agctatggtt gcttccgccc ctcaccagtt tgcgcaagaa
     1261 aattactttg ttatcgattt cctttaccgt aacattataa ttttttattc ggtcgaacag
     1321 gtgttccatt tcgttcagct cgtg
//
LOCUS       DAOPUW010000615         1343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_114312_length_1343_cov_2.270186, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000615 DAOPUW010000000
VERSION     DAOPUW010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114312_length_1343_cov_2.270186"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PKJ43_09215"
     CDS             complement(<1..304)
                     /locus_tag="PKJ43_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72791.1"
                     /translation="MAVSYITRNNKRVLFIEYKDCKSVEDTLRVLDQIKTEFLKTEGV
                     WLTLHDFSNGFGSNEFMKKASQYANEYFNKRPALNAAIGVSGLKKILLQGYNLVVKD"
     gene            complement(443..>1343)
                     /locus_tag="PKJ43_09220"
     CDS             complement(443..>1343)
                     /locus_tag="PKJ43_09220"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422269.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNZ72792.1"
                     /translation="MREMILASTTEGRKKALAKLLPFQRADFEGILEAMAGFGVTIRL
                     LDPPLHEFVPHQLATQKELADEMGISLDAVKIKVAELEEFNPMLGHRGCRLGITYPEI
                     TEMQARAIIEAALNLKAKGIDARPEIMVPLVGTLAELKNQSEIIKKTAEKVFEEKGDR
                     IDFLVGTMIEIPRAAVTADQIATEADFFSFGTNDLTQMTLGFSRDDAGKFLPEYINKG
                     IFKQDPFQVLDQTGVGLLVEMGASKGRSTKPNLKVGICGEHGGEPSSVKFCDKVGMNY
                     VSCSPFRVPIARLAAAQANIEKK"
BASE COUNT          377 a          285 c          249 g          432 t
ORIGIN      
        1 tatcttttac aaccagatta tatccttgca ataatatctt tttaagcccc gaaaccccta
       61 tggctgcatt caatgccgga cgtttgttga agtattcatt agcgtattga gaggctttct
      121 tcataaattc attactccca aaaccgttcg aaaaatcgtg caacgttaac cagacacctt
      181 cggttttaag gaactctgtt ttaatttggt cgagaacccg taatgtatct tcaactgatt
      241 tacaatcttt atactcaata aaaagtactc ttttgttatt tctggtgatg tatgaaactg
      301 ccatgatgtg atagaataat ttaattttcg ataaagttat aataaacagt tttcttataa
      361 aacatcacta gcacttattt tatcttttgc ataaaaaaac cggctcattt attgaaccgg
      421 tttttcatta tagcacaata ggttatttct tttcaatatt tgcctgtgca gcagctaagc
      481 gggcaattgg aaccctgaaa ggcgaacaac ttacgtaatt cattcctact ttatcacaga
      541 atttaactga agatggttca ccaccatgtt cgccgcaaat acctactttt aagttaggtt
      601 tagtagaacg gcctttgctg gctcccattt caaccaaaag cccaacacct gtttgatcga
      661 gaacctggaa cgggtcttgt ttgaaaatgc ctttattgat atattccggg aggaattttc
      721 cagcatcgtc gcgagagaac cctaaggtca tctgagttaa gtcgttagtt ccgaaagaga
      781 agaagtcagc ttcagtagca atttgatcgg cagttacagc tgcccttgga atttcaatca
      841 tggtgcctac aaggaaatca atacggtcac ctttttcttc gaatactttt tcagcagttt
      901 tcttaatgat ttccgactga tttttcaact cggccagagt acctacaaga ggaaccatta
      961 tttccggacg ggcatcaata ccttttgctt tcaggttaag agctgcttca ataatagccc
     1021 gggcctgcat ttctgtaatc tctggatagg taatacctaa gcggcatcca cggtgaccaa
     1081 gcattgggtt gaattcttcg agttcggcaa ctttaatttt cactgcatca agtgaaattc
     1141 ccatctcgtc ggctaattct ttttgagtag ccaattgatg aggaacaaat tcgtgcaatg
     1201 gtgggtcgag taaacgaata gttacaccga atcctgccat agcttccaaa attccttcaa
     1261 agtcggcacg ttggaaaggc aacagttttg ccaacgcttt cttcctgcct tctgttgttg
     1321 atgcaaggat catttcgcgc atg
//
LOCUS       DAOPUW010000616         1343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_114314_length_1343_cov_2.257764, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000616 DAOPUW010000000
VERSION     DAOPUW010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114314_length_1343_cov_2.257764"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1343)
                     /locus_tag="PKJ43_09225"
     CDS             complement(<1..>1343)
                     /locus_tag="PKJ43_09225"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNZ72793.1"
                     /translation="SVSEGQIRFTTDGSVPDKNSSLYFGPIQISATTVVRAVWIGNEG
                     VDNPVITSTYIFEEPSGLPVISLSTDPKNFFDWYEGILVDGPNASPDDPHYGANYWMD
                     WEKPVFMEYFNANSQLKIAQGAGVQVTGNWSRANPQKSLALFARKEFGKGSFDYQFFH
                     DRPNQSFESFILRNSGNDWWYTMFRDGLSSEIARNMNMERLAFQPTRVYLNGEYWGIL
                     NMREKPNEHYFFDSYGVKEENLLLLDNNGSVIQGSSQEYNKLRNIISVSSLEEESKYA
                     RVRSMLDIECFIDYQILEIFIDNRDWPGNNIKFWKAAGDAGRWRYLLFDSDFGYNIYG
                     ANGNTLEFATEPYGPSWPNPPWSTLFLRKLLVNNDFKNQFITRFSDCLNSTFVVENLT
                     DKVDSIVRIISREIPSHLGRWYFDYENWKMNVSTVKSFIYGRRADMREYIREYFG"
BASE COUNT          423 a          315 c          245 g          360 t
ORIGIN      
        1 cccaaaatat tccctgatat attctcgcat atctgcacgc cgcccataaa taaaggattt
       61 caccgttgaa acattcattt tccaattttc gtaatcgaaa taccaccgtc caagatgaga
      121 cggaatttcc cttgaaatta tacgaacaat tgaatcaact ttgtctgtaa gattttcgac
      181 aacaaaagtg ctgttcaaac aatcggaaaa acgcgtaata aactgattct taaaatcatt
      241 gttcaccagt aatttacgaa ggaaaagagt agaccaggga gggttgggcc acgatgggcc
      301 ataaggttca gtggcaaact caagcgtatt cccgtttgcc ccataaatat tatacccaaa
      361 atcgctgtca aacaacaaat aacgccaacg accggcatct ccggctgctt tccaaaactt
      421 aatattattt ccgggccagt cgcggttatc gataaaaatt tcaagaatct gataatcaat
      481 aaaacattca atatcaagca tggaacgaac acgggcatac ttactctcct cttcgagcga
      541 agaaacagag ataatattgc gcaacttatt gtattcctga ctactcccct gaatgacact
      601 tccattgtta tcgagcaaca acaggttctc ttcttttact ccgtagctat caaaaaagta
      661 atgctcgtta ggtttttccc tcatgttaag aattccccag tactccccgt tcaggtaaac
      721 acgagtgggc tgaaaagcaa gcctttccat gttcatgttg cgcgcaattt ctgaacttaa
      781 tccgtcgcga aacatagtat accaccaatc gtttcccgaa ttgcgaagaa taaatgattc
      841 gaaagattgg tttgggcgat cgtgaaaaaa ttgataatcg aacgatccct tcccaaattc
      901 tttgcgtgca aaaagagcca gcgatttttg agggtttgcc ctcgaccagt taccagtcac
      961 ctgtactcct gccccttgag caattttcaa ctgagaatta gcattaaaat actccataaa
     1021 aacgggtttc tcccaatcca tccaataatt agccccataa tgaggatcat cgggagacgc
     1081 gttggggcca tcgactaaga tgccttcata ccaatcgaaa aaattctttg gatcggtaga
     1141 aagtgaaatt acaggcaagc ccgaaggctc ttcaaatatg taggtcgatg taataaccgg
     1201 gttatcaaca ccttcatttc caatccaaac tgcacggact accgttgtcg ccgaaatttg
     1261 aatcggtcca aaatataagc tactgttttt gtcaggtacc gaaccatcag ttgtgaagcg
     1321 aatttggcct tcggaaacag acc
//
LOCUS       DAOPUW010000617         1341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_114540_length_1341_cov_2.569984, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000617 DAOPUW010000000
VERSION     DAOPUW010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114540_length_1341_cov_2.569984"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /gene="pdxA"
                     /locus_tag="PKJ43_09230"
     CDS             complement(<1..850)
                     /gene="pdxA"
                     /locus_tag="PKJ43_09230"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNZ72794.1"
                     /translation="MKNNQIIVGITQGDINGIGYEVIIKALSDTRILELCTPVIYGSP
                     KVAAYHKKVIEAETVNFSHVRSADEIDIDRYNIINCCDDNVRVELGKVSDVAGEAAYL
                     ALEQAVEDLRAGKIDALVTAPVSKEAIQSNKFTFPGHTEYLADKFGVKDPLMLLIADG
                     LRVGVVVGHTALKDVASKITKERIVSKLKILHRSLISDFACTNPRIAVLGLNPHNGDN
                     GLLGDEEIRIIKPALHEAESMGITALGPYPADGFFGSGNFQKFDAILAMYHDQGLTPF
                     KALTFDR"
     gene            complement(1108..>1341)
                     /locus_tag="PKJ43_09235"
     CDS             complement(1108..>1341)
                     /locus_tag="PKJ43_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HNZ72795.1"
                     /translation="YAVEVRGLWQVENDFMGGPFVSLSILDVLKRRVVTVEGFVYAPA
                     NKKRNLLQQVEAMAYSLKFDKQKDMDKLNVQVQ"
BASE COUNT          380 a          303 c          238 g          420 t
ORIGIN      
        1 cacggtcgaa cgtaagtgct ttgaacggag tcagcccctg atcgtggtac atcgctaata
       61 tggcatcgaa tttctggaaa tttccagagc caaaaaagcc atcggcagga tatggtccca
      121 atgctgtaat gcccattgat tcggcttcgt gaagagctgg tttaataatc ctaatttcct
      181 catctccaag taatccatta tcaccattat gtggattaag tcctaatacc gcgattctgg
      241 gatttgtgca tgcaaaatcg ctaattaacg aacgatgcaa aatttttaac ttcgaaacta
      301 tacgctcttt ggtaattttt gaggcaacat ctttcagcgc agtgtgtcca acgacaaccc
      361 caacccgaag cccatcggca atgagcaaca taagcgggtc tttaactcca aacttatcgg
      421 caagatactc agtatgaccc ggaaaagtaa atttgttaga ttgaatagct tctttgctca
      481 ccggagcagt taccagcgca tcgattttac cggcacgaag gtcttcaacg gcctgttcaa
      541 gggcaagata tgcagcttcg cctgctacgt cgctcacttt gccaagctca acacgaacat
      601 tgtcgtcgca acaatttatt atattatacc tatcgatgtc aatttcatca gctgacctaa
      661 catgactaaa atttacggtt tcagcttcga tcactttttt atgataggca gcaactttgg
      721 gtgacccgta aattactggt gtgcacaatt cgagaattct tgtgtcgctg agggctttta
      781 tgataacctc gtagcctata ccattaatgt cgccttgggt tatccccaca attatctgat
      841 tgtttttcat tcgtttgaat tcattagttt taccgactac ctttattatg agtatagtac
      901 acttttttat tccctcagca taatccgtga tttagtatgc atctgtttat catacttcga
      961 tagcaagcta acatgttggt caaattttta aatagtttct atttaaaatg ctactgcatc
     1021 gggaacgcac aaaaataatt ttttttttcg attatccttt ttgccaaaaa cggaatacta
     1081 agtaaacggc ggctacaatg caaaaactta ttgcacttgc acattcagtt tatccatatc
     1141 tttttgttta tcaaacttaa gagagtatgc catcgcttct acttgttgca aaaggttacg
     1201 ttttttgttt gctggtgcat acacaaaacc ttcgactgta acaacacgcc ttttcaaaac
     1261 atcaagtatg gaaaggctca caaatggtcc acccatgaaa tcgttttcaa cctgccacaa
     1321 tccccgcact tcaacggcat a
//
LOCUS       DAOPUW010000618         1341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_114580_length_1341_cov_2.090202, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000618 DAOPUW010000000
VERSION     DAOPUW010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114580_length_1341_cov_2.090202"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PKJ43_09240"
     CDS             complement(<1..624)
                     /locus_tag="PKJ43_09240"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HNZ72796.1"
                     /translation="MKITTSIKILAIVWHIIISGTIQLSAQTVWLDELDLSTATQGYG
                     VPMKNKSCDGKTLTIAGETFERGFGSHSESLLTILLEGNAKLFTAKVGIDDEVKGQRP
                     AAEFIVNGDGKQLWSSGVMRLGDAAKPCSVKLDGVKKIELLVTDGGNGNYYDHVDWVD
                     AKFETNGATTFKTYNPVSSEIYILTPKPAATPKLTGAKVFGVRPGSPF"
     gene            complement(677..>1341)
                     /locus_tag="PKJ43_09245"
     CDS             complement(677..>1341)
                     /locus_tag="PKJ43_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HNZ72797.1"
                     /translation="EYKGMIVDASELTRGNHPGIIEFKGKSYCFGHSYDMLKKTTSKF
                     YERRSVDMDEMVYNADGTIQNRKYWSVEGPTQVGIFNPFKRVEAETMAWSEGLKTNFE
                     TEWEGPFEWNRGKKIADRLYVTSIHNGDYILVQGVDFAQGAKSVEAMVSPLYGGKIEI
                     HTDKIDGPVIATVNVTTSGEGDIWKTNSAPVSNVQGVHDIYFVFRGEKDLFNFDWWQF
                     SK"
BASE COUNT          343 a          322 c          267 g          409 t
ORIGIN      
        1 aaatggcgaa ccaggacgaa caccaaaaac cttggctccg gtcaattttg gcgtagctgc
       61 aggttttggg gtgagtatgt aaatttcact tgaaaccggg ttgtatgttt tgaaggttgt
      121 tgcaccgttt gtttcaaatt tggcatccac ccaatcgaca tggtcgtagt agttgccgtt
      181 tccgccatcg gttaccagca attcaatctt tttcacgcca tcgagcttaa ccgagcaagg
      241 cttggcagca tcgcccaagc gcattacacc gctcgaccat agctgtttgc catcgccatt
      301 gactataaac tcggctgccg gacgctgtcc tttcacctcg tcgtcgatac ctactttggc
      361 ggtgaacaat tttgcattgc cttccaatag gatggttagc aaactttcag aatgacttcc
      421 aaagccacgt tcaaaggttt cgcctgcaat ggtaagggtt ttaccatcgc acgatttatt
      481 tttcataggt acgccgtacc cttgcgttgc agtgctaaga tcaagttcat cgagccaaac
      541 ggtttgggcg gataattgaa ttgttcctga aatgataatg tgccaaacga tggccagtat
      601 tttaatgcta gttgtaattt tcatcgatct gattttattt tctgaatatc aaattgatac
      661 atgactcact tatcggttat tttgaaaatt gccaccagtc gaaattgaat aaatcttttt
      721 cgcctctgaa aacaaagtag atatcgtgca caccctgcac attcgaaacc ggtgctgaat
      781 ttgttttcca aatatcgccc tcgcccgaag tggttacatt gacagttgca attacaggcc
      841 cgtcaatctt atcggtatga atttcgattt taccaccata aaggggcgaa accatggctt
      901 cgaccgactt ggcgccttga gcgaaatcaa ctccttgtac taaaatataa tcaccattat
      961 gaatggaagt tacatacaaa cgatcggcga ttttcttacc ccgattccat tcaaacgggc
     1021 cttcccattc ggtttcgaaa ttcgttttca gtccttcgct ccaagccata gtttcggctt
     1081 caacacgctt aaacggatta aatataccaa cttgtgtcgg cccttcaacc gaccaatatt
     1141 ttctattttg tatggttcca tcggcattgt aaaccatctc gtccatgtca accgagcgac
     1201 gctcgtaaaa tttcgatgtg gtctttttca gcatatcgta gctgtgacca aaacaatacg
     1261 attttccttt gaattcgata atacccgggt ggtttccgcg tgtgagttcc gaagcatcga
     1321 caatcattcc tttgtattcc c
//
LOCUS       DAOPUW010000619         1338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_114892_length_1338_cov_3.697584, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000619 DAOPUW010000000
VERSION     DAOPUW010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114892_length_1338_cov_3.697584"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..442
                     /locus_tag="PKJ43_09250"
     CDS             149..442
                     /locus_tag="PKJ43_09250"
                     /inference="COORDINATES: protein motif:HMM:NF026248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4491 family protein"
                     /protein_id="HNZ72798.1"
                     /translation="MSISSFAGILIGIGAFLIIGLLHPVVIKGEYYFGVKIWPWFLIG
                     GIVCIIGSLLISNIILSSLTGVLGFSLFWSIHELFEQKRRVEKGWFPRKPKKK"
     gene            complement(452..1021)
                     /locus_tag="PKJ43_09255"
     CDS             complement(452..1021)
                     /locus_tag="PKJ43_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNZ72799.1"
                     /translation="MILDLISKNRSYRRFDQTFEITETQLRDFIDLARLSASGRNAQP
                     LKYILSVDRKKNGKIFSTLGWAAYLKDWDGPVEGERPSAYIVQLLDTSIGANFFCDDG
                     IAMQSILLGAVEAGLGGCIFRTINKPTLMELLCIPDQYQIINVIALGKPIEKVVIDEI
                     KDGDFKYWRDENGVHHVPKRTVDELVLGL"
     gene            complement(1025..>1338)
                     /locus_tag="PKJ43_09260"
     CDS             complement(1025..>1338)
                     /locus_tag="PKJ43_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72800.1"
                     /translation="PNWPAICFKRKTIGSFSSNSDQSVCISLVRCWLALTKINSSLEE
                     QYNKRLLVPFFKQGLGSFSHSIAGYRWSFLKSSQSFFLFFAAALSVSIQSTIFNFVRK
                     T"
BASE COUNT          412 a          273 c          259 g          394 t
ORIGIN      
        1 atatttaagg gttttcgttg cccaattacg aaaaaagatt gaagataacc cgtcgaaacc
       61 caccctgttg gttaccgagt caggcattgg gtaccgattt ggagaatagg gaacgaattg
      121 cttcttttgc cggactaata aattcactat gtcaatctca tcttttgccg gaatccttat
      181 tggtattggc gcatttctca tcataggcct tcttcacccg gttgttatca aaggtgaata
      241 ctatttcggg gtaaaaatat ggccatggtt tcttattgga ggaatagttt gcattattgg
      301 atctttactg ataagcaata ttatcctctc gagtttaaca ggagtgttgg gtttttctct
      361 tttctggtct attcacgaac tttttgaaca gaaacgtcgt gtagaaaaag gttggttccc
      421 ccggaagccg aaaaaaaagt agcagtggaa tttacagccc aagaaccaat tcatcaaccg
      481 tgcgtttagg tacatgatgc accccatttt catcgcgcca gtatttaaaa tcgccatctt
      541 ttatttcatc aataaccact ttttctattg gctttccgag tgcaattaca ttgataattt
      601 gatattgatc aggaatgcat aacaactcca taagcgttgg cttgttgatc gttcgaaaaa
      661 tacacccccc taagccggcc tcaactgccc ccagaagaat gctttgcatg gcaataccat
      721 cgtcgcaaaa gaaatttgcc ccaatacttg tatcgagaag ctgaacgata taagcagatg
      781 ggcgttcgcc ttcaaccggg ccatcccaat ctttaagata tgcagcccat ccaagtgtgg
      841 agaaaatttt tccattcttt ttacgatcaa cagaaagaat atatttcaag ggttgagcat
      901 ttcttcctga agccgataag cgagcaaggt caataaagtc tcgcagctgg gtttctgtta
      961 tttcaaacgt ctgatcaaaa cgccgataac tgcggttttt cgaaataaga tcgagtatca
     1021 taatttaagt ttttctgaca aagttaaaaa ttgtagactg aattgatacg gataaggcag
     1081 ctgcaaaaaa tagaaaaaag gattgggagg attttaaaaa agaccaacga taacctgcaa
     1141 tagaatgtga gaaacttccc aacccttgct taaaaaacgg caccagcaat cgtttattgt
     1201 attgctcttc taatgaacta ttaatttttg tcaatgccag ccaacatcta accaacgaga
     1261 tacaaacaga ttgatcagaa ttactgctga aggaaccaat agtctttcgt ttaaagcaaa
     1321 tcgctggcca attcggca
//
LOCUS       DAOPUW010000620         1326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_116690_length_1326_cov_3.051928, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000620 DAOPUW010000000
VERSION     DAOPUW010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116690_length_1326_cov_3.051928"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1326)
                     /locus_tag="PKJ43_09265"
     CDS             complement(<1..>1326)
                     /locus_tag="PKJ43_09265"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNZ72801.1"
                     /translation="KLIEYRPAPGQLVNTEAAGSYTAAESLVGSLNGMVSLGAFGGYV
                     VYAFGAPVKNDAKNPYGVDFVIVGNVQTDWAEPGIVSVMKDENKNGKPDDTWYELAGS
                     EYFFSTTQKNISVTYANPLFSGSSDVFWQHSDGTSGVVATNEFHKQDYFPSADFFGGN
                     AVSSMMVSGTLIADQVDLSNPAMVKCYYRRFGYADNTMRTNSMANLPDNPYSEFIEGN
                     GGDAMDISWAVDNNGNYVEIDQIDFVKIQTGVLANAGWCGEISTEITGIFDVVPDETL
                     AQIPACVVIDRIAQKIILGSKLQLEAAAFVNGRFDANERITWKTDNNTLASVSANGMM
                     TALGQGNVSVTASLSDKPGVESSLSFEIVAPQRIEVLLTSPVILINEETEIKIKVFDN
                     AGKEITGLKTGFVSSDNSAFSIAERAGKFYIKGLKEGESTLDVFLVQAP"
BASE COUNT          395 a          312 c          237 g          382 t
ORIGIN      
        1 caggagcttg aactaaaaaa acgtcaagcg ttgactcacc ttcttttagt cctttgatat
       61 aaaatttccc tgccctttca gctattgaaa aagctgagtt gtccgaggat acaaaaccag
      121 ttttcagccc tgtaatctct tttccagcgt tatcgaaaac cttaatcttt atttctgttt
      181 cttcgttaat taaaataacc ggtgaagtta acaagacttc aatacgttgt ggggcaacaa
      241 tctcaaacga taatgaagat tcaacgcccg gtttatccga caacgatgct gtaacagata
      301 cattcccttg gcccaaggca gtcatcattc cgttcgctga aaccgatgcc agggtattgt
      361 tgtctgtttt ccatgttatt ctttcattgg catcgaaacg accattaacg aaagcagcag
      421 cttctagttg aagcttactt cctaaaataa tcttctgggc aatccggtca attaccacac
      481 aagcaggtat ttgagcgaga gtttcatcag gaacgacatc aaaaatccct gtgatttcgg
      541 tagaaatttc accacaccaa ccggcattag ccaaaacccc agtttgtatt ttaacaaaat
      601 caatttgatc aatttcgaca taatttccat tgttgtctac agcccatgag atatccatgg
      661 cgtcaccacc gttcccctca ataaattcac tataaggatt atctggtaaa tttgccatag
      721 aatttgtgcg catcgtattg tcggcatatc caaaccggcg ataataacat ttgaccatag
      781 ccgggtttga caaatcgact tggtcggcaa ttaatgttcc tgaaaccatc attgaactca
      841 ctgcatttcc gccaaaaaaa tcggccgatg gaaagtagtc ttgtttatgg aattcatttg
      901 ttgcaacaac accagaagtg ccgtcagagt gttgccaaaa cacatcactg ctccccgaaa
      961 aaagcggatt agcataggta acagaaatgt ttttttgggt agttgaaaag aaatattcac
     1021 ttccagccag ttcataccag gtatcatcgg gcttcccatt tttattttca tctttcatta
     1081 ccgaaacaat tccgggttcc gcccaatcgg tttgaacatt cccaacgatt acaaaatcga
     1141 caccatacgg gttttttgca tcgtttttta ccggtgctcc gaaagcatat acaacatatc
     1201 cgccaaaagc tccgagagaa accataccat taagcgaccc cacaagcgat tcagcggcag
     1261 tataacttcc agcagcctct gtatttacaa gttgaccagg agcagggcgg tattcaatta
     1321 attttg
//
LOCUS       DAOPUW010000621         1325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_116853_length_1325_cov_2.476378, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000621 DAOPUW010000000
VERSION     DAOPUW010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116853_length_1325_cov_2.476378"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PKJ43_09270"
     CDS             complement(<1..261)
                     /locus_tag="PKJ43_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HNZ72802.1"
                     /translation="MEYNTSLKKLILPEYGRNIQNMVDFIMAIEDRQQRNMAAQSIVD
                     VMGNLYPYLRDIPDFKHKLWDHLAIMSDFQLDIDYPYDLPTRE"
     gene            complement(410..>1325)
                     /locus_tag="PKJ43_09275"
     CDS             complement(410..>1325)
                     /locus_tag="PKJ43_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNZ72803.1"
                     /translation="SFKEQLVTSSAYEIALVVARQSGIVDEYYQDKSPESLSKFQNVQ
                     ELLNAIQDFSINALEEGRPNKLENYLEEIALVTDQDEDKEEDKNRVTLMTVHSAKGLE
                     FKNVFVVGLEEKLFPSSYSGTYTPESLEEERRLFYVALTRAMKNAWLSFARQRYSWGQ
                     LNFCEPSRFIEEIDERFISQKLPTHQSSSFFGRNEFLFNKQEDSRPVINEKQDIYPPR
                     RPSTGQVLPPLSKRTASLKNAMSRPVFEADDPGVITVGKLVEHQRFGVGRVLELEGEG
                     ANRKATVIFENAGRKQLLLKFARLRLIE"
BASE COUNT          376 a          295 c          251 g          403 t
ORIGIN      
        1 ttcacgcgtg ggcagatcgt aaggataatc aatatcaagc tgaaaatcag acattatagc
       61 cagatgatcc cagagtttat gcttaaaatc ggggatatcg cgtaaataag gatacaggtt
      121 tcccataacg tcgacaatgc tttgtgcagc catatttcgc tgttgacgat cttcaattgc
      181 cataataaaa tcgaccatat tttgaatatt gcgaccgtac tcgggaagta tcaatttttt
      241 cagacttgtg ttgtattcca tttttatagt tgtgtagtaa atatcggatt attagcaata
      301 tgctgtattt caatcagcca caatgctgtg caaatttagc aaacaatttg gtagattaat
      361 aattcgcatc aagttttcat caccagggaa ttaaaacacg cacgatgaat tattcaatca
      421 atcgtaaccg ggcaaatttc agtaaaagct gttttcgtcc ggcattttca aaaataactg
      481 ttgcctttcg gttggcgccc tcaccttcca actccagcac tcgaccaaca ccaaatcttt
      541 gatgttcaac caattttccc acagtaatta cccctggatc atcggcttcg aaaacgggcc
      601 tcgacatagc attcttcaat gaggcggttc gcttacttaa tggcggcaga acttgccctg
      661 ttgaaggcct tcttggagga tatatatctt gcttttcgtt tatgaccggg cggctatcct
      721 cctgtttatt aaaaagaaat tcgttccgac caaaaaacga agacgattga tgcgttggga
      781 gtttctgaga aataaaacgt tcgtcgatct cttctataaa ccggcttggc tcacagaaat
      841 taagttgtcc ccaactgtaa cgttgacggg caaacgaaag ccatgcattt ttcatggccc
      901 ttgtaagggc aacataaaat aaacgacgct cttcttcaag actttctgga gtatatgttc
      961 cgctgtaact cgacgggaaa agtttttctt cgagccccac cacaaataca tttttaaact
     1021 ctaagccctt ggccgaatga acagtcatca atgtcaccct attcttgtct tcttctttgt
     1081 cctcatcctg atcggtgacc agagcaatct cttcgagata gttttcgagt ttgttgggcc
     1141 ggccttcttc caaagcgttg atcgaaaagt cctgaatagc attcaataat tcctgaacat
     1201 tttgaaattt actaaggctt tcgggcgatt tatcctgata atattcatca acaattccac
     1261 tttgccgggc tacaaccaag gcaatctcgt atgccgatga tgtgacaagt tgctctttaa
     1321 aagac
//
LOCUS       DAOPUW010000622         1313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_118442_length_1313_cov_2.288553, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000622 DAOPUW010000000
VERSION     DAOPUW010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118442_length_1313_cov_2.288553"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PKJ43_09280"
     CDS             complement(<1..496)
                     /locus_tag="PKJ43_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72804.1"
                     /translation="MQPLQPDTSYHIFNHANGFENVFREAENYRYFLEKYRLYISPIA
                     ETYAYCLMPNHFHLVVRIRKRSVIEQLLLNSSNSTFSKVQNFGKGDGIGKGDGNEVEN
                     NSISNATIEKYLSKQFANFFSCYTQSFNKSYKRMGSLFIKNFKREPIDDKQHFFNAVI
                     YTHRN"
     gene            complement(505..>1313)
                     /locus_tag="PKJ43_09285"
     CDS             complement(505..>1313)
                     /locus_tag="PKJ43_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HNZ72805.1"
                     /translation="TAEWSGINFSKIEIYYFTNDTNEKLVATRKSLDDSFTAKKIEES
                     VTDTGIQKILLKHLENNSNKPELAFSPDGIDEMNRNMIELNDGEQHQPIYKVRVYEPM
                     GNKFNVGSTGNKSSKYVEAAKGTNLFFAIYVNEEGKRSFETIPLNIVIERQKQGLISV
                     PEKNEKGEKLLFWLSPNDLVYLPTPDEIEAGSLSGEIRIDRMYKMVSCTGYVGDFIPA
                     FVANAIVQTVELGSNNKAQRAWTNEMIKESCVPIKVDRLGNVIEINGRKL"
BASE COUNT          365 a          269 c          228 g          451 t
ORIGIN      
        1 ggttgcggtg cgtataaatt actgcgttga agaaatgttg tttatcgtcg atgggctcgc
       61 gtttaaaatt cttgataaac aacgacccca tgcgtttgta gcttttattg aacgattgcg
      121 tatagcagct aaaaaaattc gcaaattgtt tcgacaggta tttttcgata gtggcgttgc
      181 taatcgaatt attttcaacc tcgttaccat caccttttcc aataccatca ccttttccaa
      241 agttttgaac tttggaaaag gtagaattcg aagaatttaa tagtagttgt tctattacgc
      301 tgcgcttgcg tattcgaaca accagatgga aatggtttgg catcaggcaa taggcgtacg
      361 tttcggctat gggcgagata tacagccgat acttttcgag aaagtaacgg tagttctccg
      421 cctctcggaa cacattctcg aacccattgg catggttgaa aatgtggtag ctggtatcgg
      481 gttgaagggg ttgcatgtta ttatttacaa tttccttcca ttaatttcaa tcacattacc
      541 aagcctatct accttgattg gaacacaaga ttctttgatc atttcatttg tccatgcccg
      601 ttgagcttta ttattacttc ctaactctac tgtttgtaca atagcatttg ccacaaaagc
      661 aggaataaaa tcaccgacat agccggtaca actcaccatc ttatacattc tatctattct
      721 gatttctcca gataaactac ctgcttcaat ttcatctggt gttggcaaat acaccaaatc
      781 gttgggagaa agccagaaca gcaacttctc gcccttttca tttttttctg gaacagagat
      841 tagaccttgc ttttgtcgtt caatgacaat atttagtgga atagtctcaa aagatcgttt
      901 gccctcttcg ttcacataga tggcaaaaaa caagttggtt cctttggctg cctcaacata
      961 cttcgatgac ttattccctg ttgaaccaac attaaattta ttccccatcg gttcatatac
     1021 ccgaacctta taaataggtt gatgttgctc cccatcattc aactctatca tatttcgatt
     1081 catctcatca ataccatcgg gagaaaaagc caattcaggt ttgtttgaat tgttttctaa
     1141 atgtttgagt aatatttttt gaattccggt atctgtaaca ctttcttcaa ttttcttggc
     1201 agtaaaactg tcatctaaag atttacgagt tgcaaccaat ttttcattgg tgtcatttgt
     1261 aaaatagtat atctcaattt tcgagaaatt aataccactc cattctgctg ttt
//
LOCUS       DAOPUW010000623         1308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_119141_length_1308_cov_2.644054, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000623 DAOPUW010000000
VERSION     DAOPUW010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119141_length_1308_cov_2.644054"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PKJ43_09290"
     CDS             complement(<1..560)
                     /locus_tag="PKJ43_09290"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HNZ72806.1"
                     /translation="MKKLTTVLLLLGFIAACKQDPKIGSNANDPDSLYQSTPYKGMPT
                     LTNRYRAIIIPSDNPLTAEGVQLGRMLFYDPVLSADSTVSCASCHIPRFAFGDSSIVS
                     KRVFGELTTRNTPPLINLGMNKKFFFDGRKTSIEDAVADALQGEQHFNYVYSKSKLEN
                     QQRYSSLFKKAFGRPGDVTEIKVNKA"
     gene            complement(557..>1308)
                     /locus_tag="PKJ43_09295"
     CDS             complement(557..>1308)
                     /locus_tag="PKJ43_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72807.1"
                     /translation="RFFQTAYISFLSAFLFTISGCKPEETNTFKTTLNFKSTFGGEQI
                     VLNKLVTTSVGDSIKFTMSKILLSEIDLLKSDGSSVRLKDAVFFGEAQSFQNILSDNS
                     TNFRSGDFTGIRFNFGLTSTQNNIDPATATCPSAFCADYDMWWGSTLKYTNIKVEGLV
                     KPNGSSTFYSLMYHVGKPVNFRTITITQPIALSKDKDNDLTISLDLKKTLDGTNPMNL
                     SLQSERVTQTTDNEPLATKFADNIVNAFSLQ"
BASE COUNT          390 a          240 c          254 g          424 t
ORIGIN      
        1 atggctttgt tgaccttgat ttccgttaca tctccggggc gaccgaacgc ttttttaaat
       61 aaggacgaat aacgctgttg gttttccagt ttgctttttg aatacacata attgaaatgt
      121 tgttctcctt gcaaggcatc tgcaacggca tcttcaatgc tggtctttcg accatcaaaa
      181 aagaattttt tattcattcc taagttaatg agcggtggag tgtttcgagt agttaattcg
      241 ccaaatactc ttttgctaac aatactgcta tctccaaatg caaatcgggg aatatgacat
      301 gatgcgcagg aaactgtgct atcggcagaa agcacagggt cgtaaaacaa cattctcccc
      361 agttgaacac cttctgccgt taatggatta tcagaaggga ttataatggc tcgatagcga
      421 ttagttagtg tgggcatacc tttgtaaggc gtgctttgat ataaagaatc cggatcgtta
      481 gcgttagaac caattttcgg atcttgttta catgcagcaa tgaaacccaa taataggagt
      541 acagtcgtta actttttcat tggagggaaa aggcatttac aatgttatcg gcaaatttag
      601 tggctaaagg ctcattatcg gtagtttgag taactcgttc agattgcaat gaaagattca
      661 ttgggtttgt accatctaga gtttttttca aatcaagcga gatggttaaa tcgttgtctt
      721 tatcctttga caaggcaatt ggctgagtaa tggtaatggt tctaaaattc accggtttac
      781 ccacatgata catcaacgag taaaaagtac tactgccatt tggttttact aatccctcca
      841 ctttaatgtt agtgtatttt aacgttgaac cccaccacat gtcatagtct gcacaaaaag
      901 cacttggaca agttgctgtg gccggatcaa tgttgttttg agtactcgtt aatccaaaat
      961 taaatcgaat gcctgtaaaa tcacctgaac gaaaatttgt cgaattatca cttaaaatat
     1021 tctgaaagct ttgcgcttcg ccaaaaaaca cggcatcttt caatcgtaca ctacttccat
     1081 ctgatttcaa taaatcaatt tctgaaagta aaattttaga cattgtgaat ttgatagagt
     1141 cgccaacact tgttgttaca agcttattca gcactatctg ttctccgcca aaagtggatt
     1201 tgaaatttaa ggttgtcttg aaagtattgg tttcttccgg tttgcatcca cttattgtga
     1261 ataaaaatgc agaaagaaaa ctaatgtatg cggtttgaaa aaatcttt
//
LOCUS       DAOPUW010000624         1306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_119426_length_1306_cov_3.330136, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000624 DAOPUW010000000
VERSION     DAOPUW010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119426_length_1306_cov_3.330136"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..>1306
                     /locus_tag="PKJ43_09300"
     CDS             150..>1306
                     /locus_tag="PKJ43_09300"
                     /inference="COORDINATES: protein motif:HMM:NF014078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HNZ72808.1"
                     /translation="MKTISPPVSAKTIELGFSPPYNLTQNDTTSDRFYFLLEEFTKKV
                     VDTGLKHFGADIANFRETGYATNKVSDEELLLELLMTGVFWNNYKGRWRWNLPLIAPM
                     FKMLYRLRSTMLKSRVDKLRGKLGNRLLNKPGKETLTFSIDQFDNLLRWLGATGDFKM
                     EVKALSRWQDYFYFELQRNSLDLLEEVSAFASWFEDQSKIQLGSYTGNVSSFLQHNKK
                     MYAGREDQFFCTRTQVEYHMNMVGAQIMNNSLRKDFLRTEKKILLLPTCMIKNKECKA
                     KVTDNALICQHCTSDCNVSKTAREMKNSGIETVLIRHSSDFSRWLKPWANQNSTGLIG
                     TACVLNLLGGGYEMKNLNIPSQCVFLDYCGCNKHWNKAGLATRINTEQAITL"
BASE COUNT          411 a          289 c          270 g          336 t
ORIGIN      
        1 atatagtttt gcagcaatgc cttatcctat tgttttttcg gttgaaagaa tacgtttgcc
       61 tataaaaaaa caccacttcc cgattacagc cattagatca agctgcttga atttactttt
      121 gaactgccat aaaacacaaa ctttttttca tgaaaacaat atcgccacct gtttcagcaa
      181 aaacaataga actcgggttt tccccgccct ataatcttac ccagaacgac acaacatcgg
      241 ataggtttta ctttttattg gaagaattta ctaaaaaagt agttgacacg gggctgaaac
      301 atttcggtgc cgacatagcc aacttcaggg aaaccggtta cgccacgaat aaagtgtctg
      361 acgaagaact tttgctcgaa ttgttgatga caggggtttt ctggaataac tacaaagggc
      421 gctggcgatg gaacctacca cttattgccc ccatgttcaa aatgctttac cgcttacgct
      481 ccactatgtt gaagagcagg gtcgacaagc ttaggggcaa actgggcaac cgtttattga
      541 acaaacccgg caaagaaaca ctaaccttca gcatagatca atttgataac ctgttgagat
      601 ggctcggagc tacaggcgat ttcaaaatgg aagtgaaagc attaagccgt tggcaagatt
      661 acttctattt cgaattacag cgcaacagcc tcgatttatt ggaagaagtt tctgcttttg
      721 cctcatggtt cgaagatcaa tcgaaaatac aactgggctc atataccggg aatgtttcat
      781 cgttcctgca acacaacaaa aaaatgtatg ccggtcgcga agaccagttc ttctgtaccc
      841 gtacccaggt tgagtaccac atgaacatgg tgggtgccca gattatgaac aatagcctta
      901 gaaaagattt cctgcgaact gaaaaaaaga ttttactgct gcctacctgc atgattaaaa
      961 acaaagaatg taaggcaaag gtaacagaca atgcactaat ctgccagcat tgcaccagcg
     1021 attgtaatgt atcgaaaact gcccgtgaaa tgaaaaattc gggaatagaa accgttttaa
     1081 tcaggcattc atctgatttt tcgcggtggc tgaaaccctg ggccaaccag aattctaccg
     1141 gtcttatcgg aacggcttgc gtactgaacc ttttgggcgg cgggtatgaa atgaaaaacc
     1201 tcaacatccc gtcacagtgt gtattcctcg actattgtgg atgcaacaag cactggaaca
     1261 aagcaggtct ggcgacacga atcaacacag aacaggctat aacact
//
LOCUS       DAOPUW010000625         1306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_119520_length_1306_cov_1.745004, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000625 DAOPUW010000000
VERSION     DAOPUW010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119520_length_1306_cov_1.745004"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..>1306
                     /locus_tag="PKJ43_09305"
     CDS             129..>1306
                     /locus_tag="PKJ43_09305"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ72809.1"
                     /translation="MKTILSQAFNSHSSIKVFLFLFALYLCNPICTRATDRQLDSLYT
                     ILRQTNDIHVLAKTHIEIAKLFWTSNPDSAFFHIDKADGLITKTKVNDDYLNNQIIRS
                     NLSFDLGHFEEALHTMQTTLQNTNEKTDPVIVARIISNIGNCYAQKGDLSKAIENYTR
                     AISYLDENKNVSEMAVLYGRLGNLYYQNKNYNEGIVFYKKASRLFIALERRSGEAISS
                     MNIGNCYKQLQVPDSAIFYYNKALKQYKAIGNMQFNEAQCLANLGNIHAELLQTDKAL
                     KYLLNADSLFNSTQNLYSIAQVNSDIAYLYLMLDKPSLAKNRIDKCLEISKKNGYDRL
                     IETGYRLLWKYYDKLGDCSNAYEWLNKYSVIKDSIGNVSRQENIDKLLTQFETERKEK
                     "
BASE COUNT          428 a          280 c          232 g          366 t
ORIGIN      
        1 tttcaattaa ttcaattttt tgcagtaaat tgctctggct tttttcgtga aacaatcgtt
       61 cctgtgccca ttttaactgg ttgctgtatc gtaaataatt accattaacc ccttacgcca
      121 tagtgcatat gaaaaccatt ctgtcgcaag cctttaacag ccattcatca ataaaagtat
      181 ttttatttct gtttgcatta tacctgtgca atccgatttg cacacgtgcc acagatcgtc
      241 aactcgatag cctttacacc attttgaggc aaaccaacga cattcatgtt ctggctaaaa
      301 cccatattga aattgcaaaa ctgttttgga cttccaatcc cgattcggcc tttttccata
      361 tcgataaggc cgacgggctg attacgaaaa ccaaggtaaa tgatgattat ttaaacaatc
      421 aaattatccg cagcaatctc tcatttgacc ttgggcattt cgaagaagcg ctgcacacca
      481 tgcaaactac actacaaaat acaaatgaaa aaacagatcc ggttatcgtc gcaaggatta
      541 tcagtaatat tggcaattgc tacgctcaaa aaggcgattt aagtaaggcc attgaaaatt
      601 acaccagggc catcagctat ctggatgaga ataaaaacgt atctgaaatg gcagtgcttt
      661 atggccgctt gggaaatctt tactaccaga ataaaaacta taatgagggt attgttttct
      721 acaaaaaagc aagcaggctg ttcattgcgc ttgagaggcg atcaggagag gccatttcaa
      781 gtatgaacat cggtaactgc tataaacagc tgcaagttcc tgattcggcc atattttatt
      841 acaacaaagc cctaaaacag tataaagcca tagggaatat gcagttcaac gaagcgcagt
      901 gtttggcaaa tttaggaaat atacatgccg aactattgca aaccgacaaa gctttaaagt
      961 accttcttaa tgccgattct cttttcaact ccactcaaaa tctgtactcc attgcacagg
     1021 ttaatagtga tatagcctat ctttacctca tgctcgacaa accctcgcta gccaaaaacc
     1081 gcattgacaa atgccttgaa atttccaaaa agaatggcta cgaccggctt atcgaaaccg
     1141 gttatcgact cctttggaaa tattatgata agctgggcga ttgtagtaat gcctacgaat
     1201 ggcttaacaa gtattccgtt ataaaagatt ccataggcaa tgtttcccga caggagaaca
     1261 ttgataaact tttaacccaa ttcgaaacgg aacgaaaaga gaaaga
//
LOCUS       DAOPUW010000626         1305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_119566_length_1305_cov_3.090400, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000626 DAOPUW010000000
VERSION     DAOPUW010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119566_length_1305_cov_3.090400"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PKJ43_09310"
     CDS             complement(<1..201)
                     /locus_tag="PKJ43_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72810.1"
                     /translation="MAEKQIDSKISDEIIKVRGARVHNLRNINVDIPRNQLTVITGLS
                     GSGKSSLAFDTIFAEGQRRYIET"
     gene            complement(274..1200)
                     /locus_tag="PKJ43_09315"
     CDS             complement(274..1200)
                     /locus_tag="PKJ43_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNZ72811.1"
                     /translation="MINKKILLWPSFVIGSILIPAALAGSSGCIDGHKVQTDTLQVVT
                     SPPIPEKLAFAGEEVPLHNFDVRENLDRELMVNAYFHSQTLRFLKLAPRYFSIIEPIL
                     KKESIPDDFKYLALAESGFDPRIVSPSGAAGIWQFMKTTGKQYGLEVNEEVDERFNIE
                     KATYAACKYLNESYAKYGNWTLVAASYNTGRGNVDRFTRMQKEDSYYDLFLVEETNRY
                     VYRILALKLILESPEEYGFFITDKEKYPVVPYELVEVKSAVPSLADFAKQQGINYKLL
                     KMYNPWLRDTSLKNVSGKTYQVKIPTGKYREF"
BASE COUNT          365 a          287 c          267 g          386 t
ORIGIN      
        1 ggtctcaatg tagcgccttt gcccctcggc aaaaatggta tcgaaagcca acgacgattt
       61 tccgctgcca ctaagcccag taataaccgt tagttggttt cgtggtatat caacattgat
      121 gttccgaaga ttgtgcaccc tggccccacg caccttgatg atttcatcgg atatcttact
      181 atctatttgt ttttcagcca ttctttaaaa ataaaaattg ttttgcccgg tgattgtatg
      241 aggatgtgtt tcaagcagca aatctaaccg tggttaaaat tcgcgatatt ttcctgttgg
      301 aattttcacc tgataggttt tccccgaaac attctttagt gaagtgtcgc gcaaccacgg
      361 gttatacatt ttcagcaact tataattgat tccctgctgc ttggcaaaat cggcgagact
      421 ggggactgca cttttcacct caacaagctc atatggcact accgggtact tttccttgtc
      481 ggtaatgaaa aaaccatatt cttcaggcga ttcgaggatt agttttaacg caagtatgcg
      541 ataaacatac cggttggtct cttcaacaag gaacaaatcg tagtaactgt cttctttctg
      601 catgcgggta aagcggtcaa catttccacg cccggtgttg tacgaggctg ccacgagagt
      661 ccaattcccg tattttgcat acgattcgtt aagatatttg cacgctgcat acgtagcctt
      721 ttcgatgttg aaccgttcat caacttcctc attcacttcg aggccatatt gctttccggt
      781 agttttcata aactgccata taccagcggc acccgagggc gaaacaatgc gcggatcgaa
      841 accgctttcg gcaagggcaa ggtatttgaa atcatcgggg atagattctt ttttcaaaat
      901 tggttcgata atagagaaat atcgcggcgc caatttcaag aaacgtaaag tttgcgaatg
      961 aaaataagca ttcaccatta actcccggtc gaggttttcg cgcacatcga aattatgaag
     1021 cggcacttct tccccggcaa aagccagttt ctcgggaata ggaggagaag taactacctg
     1081 aagcgtatct gtttgaacct tgtgaccatc gatacaaccc gaagaacctg cgagtgcagc
     1141 aggaattaaa atgctcccga tgacaaatga tggccaaagc aatatttttt tgtttatcat
     1201 agtattatca tttctttgtt tgttattttg caatcgacaa aaataatcgc tttaaaccaa
     1261 aataaattgt gaaccaatgg aaaattcatt cgataaaaag gcttc
//
LOCUS       DAOPUW010000627         1303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_119850_length_1303_cov_4.298077, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000627 DAOPUW010000000
VERSION     DAOPUW010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119850_length_1303_cov_4.298077"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PKJ43_09320"
     CDS             <1..361
                     /locus_tag="PKJ43_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNZ72812.1"
                     /translation="ASVLSHPTEYLQPLRWVGIVSGHVSCDIAASTGIHDYQTALKMI
                     MAGASAVQVVSGVYKHGYDVVKHMVHGMDKWLDQNGIASLSELIGCAGSSKVKNPAMY
                     ERVQFMKYFGEFDQPVD"
     gene            complement(358..>1303)
                     /locus_tag="PKJ43_09325"
     CDS             complement(358..>1303)
                     /locus_tag="PKJ43_09325"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNZ72813.1"
                     /translation="LCEEVIYYKRSMSVLNQLSVKPFIVKSRANRKLLRNLQHDNYPI
                     LFEGLHTCYYLTHKKLADRIKIVRCHNIEHDYYDSLTRGVKSLLLLAYFKTEAGKLKR
                     FEKSLEHATAIAAISEADKNYFSSKYGKTFLMRPCHPSAQVLAQKGTGEYILYHGDLS
                     TAENIDSALFIIQDIVPGIKQKLIIAGKNPSKKITTAASQHDTIQVIANPTHDELQLL
                     ITQAHVNLLPTRQATGFKLKLMNALHNGRFCVVTSEMVKGTGLETACIVATSADEICA
                     ILEDLMRTPFSEEDIEKRKALLEPFSNNASIAHLLQII"
BASE COUNT          333 a          257 c          300 g          413 t
ORIGIN      
        1 ggccagtgtt ttgagccatc ctaccgaata tttgcaacct ttgcgttggg tgggtattgt
       61 gtcgggacat gtttcgtgcg acattgccgc cagcaccgga attcacgatt accaaactgc
      121 cctgaaaatg attatggcag gagccagtgc ggttcaggtt gtttcggggg tttacaaaca
      181 tggatacgat gttgtgaaac acatggttca tggtatggat aagtggctcg accagaatgg
      241 aattgcctca ctttctgagc taattggctg cgctggatcg tcaaaagtga aaaatccggc
      301 catgtacgag cgtgttcagt ttatgaaata ttttggtgaa ttcgatcagc cggtcgacta
      361 gataatttgc agtaaatgag caattgaagc attgtttgaa aacggttcaa gcaatgcttt
      421 tcttttttca atatcttctt cgctgaaagg ggttctcatc aaatcttcca gaattgcaca
      481 tatctcatct gcagatgtgg ctacaataca cgcggtctca agccctgtgc ctttaaccat
      541 ttcggaggtt acaacacaga aacgtccgtt gtgcaacgca ttcatcaatt ttagtttgaa
      601 tcctgtggcc tgtcgggtag gcagcaaatt tacatgggct tgcgtgataa gcaattgcag
      661 ttcatcgtgc gttgggttgg caattacttg aatagtatcg tgttgactgg ctgccgtagt
      721 gattttcttc gatgggtttt tccctgcaat aatcaatttt tgttttatcc ctggtacaat
      781 atcctgaatg atgaaaagtg cagaatcgat attttcagcg gtacttagat cgccgtggta
      841 taatatgtat tcaccggtcc ctttttgcgc cagtacttgt gctgagggat ggcaaggtct
      901 catcaggaag gttttcccgt atttcgaact gaaataattt ttgtcggctt ccgaaattgc
      961 agcaattgct gtagcatgtt caaggctttt ttcaaagcgt ttcaattttc cagcttcggt
     1021 tttaaaatag gccaaaagca ataatgattt tactccgcgg gttagcgaat cgtaataatc
     1081 atgctcaatg ttatggcacc gcactatttt aattctatcg gccaattttt tgtgtgtaag
     1141 atagtagcac gtatggaggc cttcgaaaag gataggataa ttgtcatgct gcaagtttct
     1201 gagcaactta cgatttgccc ggcttttcac gataaagggt ttcaccgaca attggttgag
     1261 taccgacatc gaacgcttat aataaattac ctcttcacat aaa
//
LOCUS       DAOPUW010000628         1303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_119889_length_1303_cov_2.878205, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000628 DAOPUW010000000
VERSION     DAOPUW010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119889_length_1303_cov_2.878205"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1303)
                     /locus_tag="PKJ43_09330"
     CDS             complement(<1..>1303)
                     /locus_tag="PKJ43_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HNZ72814.1"
                     /translation="YIAGVYYPDKTRVGWWKNGYPEYFAKVLNAANWIGINVKVDGEE
                     LDLAHMIIESFQRVLNMKEGTLSRSFVASFKSGKQLKVTSLRFLSIVTPEVGAIRYTV
                     QPLNFSGKIKVTPFVDFDVINEDSNYDEKFWVGEYLENLGNEGYLTAHTKKTKFLVCS
                     GMKYNVLLDGKEHAVHIENESREKYIESSFTLDLKQKQEITLFKYGVNTSSEYFNKSR
                     LAYESKEILNEAFETGFEALLGEHKETWARKWETSDIKIKGDVAAQQGIRFNIFQLNQ
                     TYTGDDERLNIGPKGFTGEKYGGSTYWDTEAYCFPFYLATAPQKVAKNLLVYRYKHLE
                     KAIENAQKLGFTGGAALYPMVTINGEECHNEWEITFEEIHRNGAIAYAIHDYIRHTGD
                     KDYLAEYGFEVLLAMSRFWSQRVNWSAQKKQFVLLGVTRPN"
BASE COUNT          321 a          306 c          266 g          410 t
ORIGIN      
        1 tcatttggcc gggtgacacc aagtaacacg aactgctttt tctgtgcaga ccagttaacc
       61 cgttggctcc agaaacgtga catagccagt aacacttcaa agccatattc tgcaaggtaa
      121 tccttgtcgc ccgtatgcct tatatagtcg tgaatggcat acgcaatggc tccattgcgg
      181 tgtatctctt cgaaggtaat ttcccattcg ttgtggcact cttctccatt gatggtcacc
      241 atgggataaa gtgcagctcc accagtaaat ccaagctttt gtgcattttc gatagctttt
      301 tccaggtgtt tataacgata aaccaacagg tttttggcaa ctttttgcgg tgcagtagca
      361 aggtagaacg ggaagcagta ggcctcggta tcccaatagg tacttccacc atatttctcg
      421 cccgtaaacc ctttcgggcc aatatttaat cgctcatcat cgccagtgta ggtttggttc
      481 aattggaaaa tattgaaccg gatgccctgt tgtgcagcca cgtcgccttt gatttttata
      541 tcactggttt cccatttcct cgcccaggtt tccttatgct ctcctagtaa tgcctcaaaa
      601 cctgtctcaa aagcttcgtt taaaatctct ttcgactcgt aggctaagcg cgacttattg
      661 aaatattcgc tcgaggtatt caccccgtat ttgaaaaggg taatctcttg tttttgtttc
      721 agatcgaggg taaacgatga ttcgatgtac ttttcgcgtg actcattttc aatatgtact
      781 gcatgttcct ttccgtcgag caacacattg tatttcattc ccgagcatac aagaaacttc
      841 gttttcttgg tgtgtgcagt caggtagcct tcgtttccaa ggttctcaag gtattcgccc
      901 acccaaaatt tctcgtcgta gttcgagtct tcattgatca cgtcgaaatc gacaaaaggg
      961 gttactttaa tttttccact gaaatttaga ggttgaacgg tgtatctgat tgcccctact
     1021 tcgggagtaa cgatgcttaa aaatcgcagc gaagtaactt ttaattgttt gccgctttta
     1081 aacgaggcca caaaagaacg ggaaagtgta ccctctttca tattgagcac tcgctggaac
     1141 gattcgatga tcatgtgggc aaggtcgagt tcttcgccat caactttcac gttgattcca
     1201 atccagttag ctgcattcaa cacttttgca aaatactcag gatatccgtt tttccaccag
     1261 cctacacgtg ttttgtcggg ataataaacg cctgcaatgt aat
//
LOCUS       DAOPUW010000629         1300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_120280_length_1300_cov_2.814458, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000629 DAOPUW010000000
VERSION     DAOPUW010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120280_length_1300_cov_2.814458"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PKJ43_09335"
     CDS             complement(<1..342)
                     /locus_tag="PKJ43_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNZ72815.1"
                     /translation="MAEQKNYALIVAGGSGSRMNATVPKQFLLLNDKPVLMHTIERFH
                     EYDNAMEIIVVLPESQFPLWKNLCREYKFRIQHQLVAGGSVRFESVKNGLSKIQGDGI
                     VAIHDGVRPLVS"
     gene            422..1081
                     /locus_tag="PKJ43_09340"
     CDS             422..1081
                     /locus_tag="PKJ43_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HNZ72816.1"
                     /translation="MKEIYHFLQQLEANNNKDWFDKNRDEFKKAQQKISHLTELLINE
                     VRSFDKEIGFLQPKDCMYRIYRDLRFSADKTPYKTNMGAYIAKNGKCGGYPGYYFHIQ
                     PNGSFVSGGLYMPNPAFLKAIRNEIYYHSDEFIDIIEEPEFKSRFSFFDEDKLKTNPK
                     GFPADFEHIELLRFKSIAPSVNVSEEQLFSNDIIDFIIENFRKFYPLNAFLYEAISKI
                     E"
BASE COUNT          427 a          257 c          237 g          379 t
ORIGIN      
        1 gctcaccaat gggcgaacgc catcgtgaat ggcaacaatt ccgtcgccct gaattttcga
       61 cagcccgttc tttacacttt caaaccgtac tgatccacct gcaaccaact gatgctgaat
      121 gcgaaactta tattcgcggc aaagattttt ccataacggg aactgggatt cgggaagaac
      181 aacaatgatt tccattgcat tatcgtactc gtgaaaacgc tcgatggtat gcattaacac
      241 cggtttgtca ttcagcaaaa ggaattgttt gggaacagtg gcgttcatgc ggctgccact
      301 tcctccggca acaatcaatg catagttttt ttgttcagcc attagaaata aagtttgaaa
      361 gtaaaatcct taggctaatt cgttaatttt gcctttgaat aaataacaaa tttatcaaaa
      421 aatgaaagag atctatcact ttttgcaaca actggaagca aataacaata aagactggtt
      481 tgacaaaaac cgtgatgaat ttaaaaaagc acaacagaag atttcacacc ttaccgagct
      541 acttatcaac gaagtacgaa gcttcgacaa agagatagga ttcttgcaac ccaaagattg
      601 tatgtaccgg atttatcgcg accttcgttt ctcggccgat aaaacccctt acaaaacaaa
      661 catgggggct tacatcgcca agaatgggaa atgtggtggt tatcccgggt attactttca
      721 tattcaacct aatggaagtt ttgttagtgg cggcttgtat atgcccaatc cggctttcct
      781 gaaagctatc agaaatgaaa tttattacca ttccgatgaa tttattgata taattgagga
      841 acctgaattt aaaagccggt tttcgttttt cgacgaagat aagttgaaaa ctaatcccaa
      901 aggttttcct gccgattttg aacacattga actcttgagg tttaagtcta ttgccccttc
      961 tgttaatgtt tctgaagaac agctttttag taatgatatc attgatttca ttatcgaaaa
     1021 tttcagaaag ttctatccgt tgaatgcatt tttatatgaa gctatttcaa aaatagaata
     1081 atacccacaa gtaaatggct tagttaattt gttggttcat tgcatttttc tttcaaatcg
     1141 agaaaaaagc aaccagataa taaacccatg aaatactgtt gcaccagcaa ggtaaagcca
     1201 ccgatattcc tgaagtgaaa aaatggctct ttagatccac caaagaggcg aaaatgaaag
     1261 gatccaacgc caatttccct gcgtaaaata atcgattaac
//
LOCUS       DAOPUW010000630         1298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_120576_length_1298_cov_2.924377, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000630 DAOPUW010000000
VERSION     DAOPUW010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120576_length_1298_cov_2.924377"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PKJ43_09345"
     CDS             <1..182
                     /locus_tag="PKJ43_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72817.1"
                     /translation="SIFPLTWWKELFIAQSLYGGPIKDSMVYICYILLFQVVSLAFLP
                     AYKKYLSDKKYWGKA"
     gene            330..>1298
                     /locus_tag="PKJ43_09350"
     CDS             330..>1298
                     /locus_tag="PKJ43_09350"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ72818.1"
                     /translation="MRRFFQHTSESLELLTHHFLHELKAIVSDKGAILILFIAVVIYP
                     VIYSIVYSSETVTNLPVAIVDQDQSAASRKYAQMLDASAEVNVVAHPTDLQLAEKLLK
                     DSKVSGVLFIPEGFEKQLLKGQQADVSLYADAAYFLKYRNELMAVTYTNAYFSAGISI
                     KKYMAAGQDYKQALISNSPLDPQAHILYNPGSGYGSFVMPGIMLIVIQQTLLIGIGLM
                     GGSFSESKKSPFKLAPHERKREIMPHLFGKAGAYLLASLFNVAFTLVIVYNWFNYTDK
                     ADFLHVLMLIFPFLVAVVFLGIGISTLFIHRESAIVFMVFLSPIALF"
BASE COUNT          370 a          278 c          252 g          398 t
ORIGIN      
        1 catcgatctt cccgcttacc tggtggaaag aactctttat tgctcaatcg ctttatggcg
       61 gaccaataaa agattcgatg gtttacatct gctacatttt attgtttcag gttgtttcgc
      121 ttgcattttt gccggcctac aaaaaatacc tgtcggataa aaaatattgg ggcaaggctt
      181 aattaattga tgatttatta ttgactactt acgattttca ttcaatacag aatatcaatg
      241 cggtcaacaa aaactttaaa tacgatacta aatcaatatc agcacttaac tagggtagat
      301 ttctaattaa cgaattaatt cagaataaaa tgagacgttt ttttcaacat acatcagaaa
      361 gccttgaact tttaacccat cactttttac acgagctgaa ggctattgtg tcggacaaag
      421 gggcaatact gattttgttt attgcagtgg taatttaccc ggtaatttat tcgatcgttt
      481 actctagcga aacagttacc aacctacctg ttgcaattgt cgatcaagac caatcggcag
      541 cgagccgcaa atatgctcaa atgctcgacg cttcggcaga ggttaatgtt gtagcccacc
      601 ctaccgactt gcaattggcc gaaaaactat tgaaagacag caaagtcagc ggggtactct
      661 tcattcctga aggtttcgaa aagcaactat tgaaagggca acaggccgat gtgtcgctct
      721 atgccgatgc ggcctatttt ttgaaatacc gcaacgaatt gatggctgta acttacacaa
      781 atgcgtactt cagcgctggg atatcgatta aaaaatacat ggcagcaggg caagattata
      841 agcaagccct gataagtaac tcgccactcg accctcaggc acacatcctt tacaacccgg
      901 gctcgggata tggttcattt gtaatgcctg gcattatgct cattgttatt caacaaacat
      961 tgttgattgg cattggactt atgggtggtt cattctccga gtcgaagaaa tcgccattca
     1021 aactggctcc acatgaacgg aaacgcgaaa taatgcctca cctattcggc aaagcaggag
     1081 catatctttt agcatctctg ttcaatgtgg ctttcacgct tgtcattgtt tacaactggt
     1141 tcaattatac cgacaaagcc gactttctgc atgtattgat gcttatattc ccatttctgg
     1201 ttgcagtagt ttttctgggc attggcattt caactttgtt catacaccgc gaatcggcaa
     1261 ttgtattcat ggttttctta tcgccaattg ctctattc
//
LOCUS       DAOPUW010000631         1291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_121627_length_1291_cov_2.410194, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000631 DAOPUW010000000
VERSION     DAOPUW010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121627_length_1291_cov_2.410194"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..530
                     /locus_tag="PKJ43_09355"
     CDS             105..530
                     /locus_tag="PKJ43_09355"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNZ72819.1"
                     /translation="MNRIKQLFRVNQAPLSTDIALLLIRLFFGGMMLTHGIPKLEMLF
                     SSATVQFIPLFGFSAGFSLALAAAAEAGGAILLIAGLGTRLAAIPMAATMLIAAFVVH
                     ANDPLATKELAILYLVAFTALIFAGSGRFSIDRFISQRK"
     gene            800..>1291
                     /gene="rsgA"
                     /locus_tag="PKJ43_09360"
     CDS             800..>1291
                     /gene="rsgA"
                     /locus_tag="PKJ43_09360"
                     /inference="COORDINATES: protein motif:HMM:NF015170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase RsgA"
                     /protein_id="HNZ72820.1"
                     /translation="MQLQEMGFSPKVEKLAHDLAFDSFSLGRVISEHKERYVVATENG
                     DVDAEITGNLRFTAQCREDFPAVGDWVALIQCDQQFAVIHKIVPRFSAMKRQSVKNRS
                     ESQIIASNIDVALLVQSVDRDFNINRLERYLAICYSSTIEPIIVFTKVDLYDKEHLSK
                     IISH"
BASE COUNT          393 a          271 c          247 g          380 t
ORIGIN      
        1 aataattgca gaaaagacag gaatgttgcg ctttgtgcat ggcaaagaga acaaaagcat
       61 tctttatttt gtattattct aaataacatt taaaaacgta gaatatgaat cggataaaac
      121 aattattcag agtgaaccaa gctcctcttt cgaccgatat tgccttgttg cttatccggc
      181 ttttttttgg aggaatgatg cttacccatg ggattccaaa gttagaaatg cttttttcaa
      241 gtgctacggt tcagtttatt ccacttttcg ggtttagtgc tggtttttcg ttagcattgg
      301 ctgcagcggc cgaagcaggg ggcgcgatat tgcttattgc aggccttggc acacgactgg
      361 cagccattcc gatggctgcc accatgctga tagccgcttt tgtggttcat gccaacgatc
      421 cgctggctac caaagaacta gctattctgt atcttgttgc atttaccgcc ttgatctttg
      481 ccggaagtgg acgcttctcg atcgaccgct ttataagcca aagaaaatga gaaagcattt
      541 ctcctgaatt attcgaaatt tgaaaataaa ccagatatac caatccaatg tattatatta
      601 caatacattg taaattttta attcttcaca gataacccat ttaacttctc cacttttcaa
      661 atattgctaa aatcccgtca gcaatattta ttatttttat attgcatcgt aaaacaatta
      721 tcatagtgaa aacccggcaa aagacaccgg gaaagtcttt caaattaaac aaagtaacaa
      781 tataaccagc acataaagca tgcagctaca agaaatggga ttcagtccta aagttgaaaa
      841 acttgctcac gatttagcat tcgacagctt ttcgctcggt cgggtcattt cagagcacaa
      901 ggaacgttac gtggtagcca ccgaaaacgg cgatgttgat gccgaaatca caggaaatct
      961 gcgttttaca gcccaatgcc gcgaagactt tcctgctgtt ggcgattggg ttgccttaat
     1021 tcaatgcgac caacaatttg cagtgataca caaaattgtg ccccgctttt cagccatgaa
     1081 acggcaatcg gtaaagaacc ggagcgagtc gcaaatcata gccagtaata tcgatgtagc
     1141 cttgcttgtc caatcggtcg acagggattt taatattaac aggctcgaaa gatatcttgc
     1201 catttgctat tcttcaacca ttgaaccaat tatagttttc acaaaagtcg atttatacga
     1261 taaagaacac ctttcaaaaa taatttcgca c
//
LOCUS       DAOPUW010000632         1285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_122446_length_1285_cov_2.990244, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000632 DAOPUW010000000
VERSION     DAOPUW010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122446_length_1285_cov_2.990244"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..548
                     /locus_tag="PKJ43_09365"
     CDS             144..548
                     /locus_tag="PKJ43_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72821.1"
                     /translation="MIKKDYLMRLLKEFLDALAELLYHVKNENNEQILARSEEFYPKY
                     LKQKRSFFIENNISAIVPSIISENEEESAIRCEMLAELLFIDAKQTNNIDIQHDLYQK
                     ALFFYDYTEQHSTTFSENRLLKIETLKSILNQ"
     gene            complement(575..>1285)
                     /locus_tag="PKJ43_09370"
     CDS             complement(575..>1285)
                     /locus_tag="PKJ43_09370"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNZ72822.1"
                     /translation="KTNGLPFEMLARCTPLSVLVKHKHNLFQLEALLFGQSGLLNEEL
                     LGDDYYLNLREEYSYFARKYGLKGMEGYLWKFMRLRPANFPTLRIAQFAALVFKSESL
                     LSKLVEESALDKIIDFFDVKASPYWDTHYRFSLPSPQGEKNLGMASRNNIIINTVAPF
                     LFLYGERNNIALLKQRALELLEALPAEHNSVISRWNEVGIQAHNAFDSQALIQLKNRY
                     CEPTKCLQCMLSTKVISR"
BASE COUNT          398 a          293 c          265 g          329 t
ORIGIN      
        1 cagaaacaat tccatgtaaa cctacagtgc gtaatagcga agaaacagag atgcatcatg
       61 ttcatcttct agcatgcaaa aaatttaggc atggccatat catcataatt gattaatatt
      121 accacctaaa cagcaaaatt atcatgataa aaaaagatta tctcatgcgg cttctaaagg
      181 aattcctcga tgcattggca gagttgcttt accatgtaaa aaacgaaaac aatgagcaaa
      241 ttcttgcccg cagcgaggaa ttctacccta aatacctaaa gcaaaaacgc tcctttttca
      301 tcgaaaacaa cattagcgcc attgtgccgt caattatttc agagaatgag gaagagtcgg
      361 cgataagatg cgaaatgctg gccgaactgc ttttcatcga tgccaaacag acaaataata
      421 ttgatattca gcacgatttg tatcaaaagg cattattctt ttatgactat accgaacagc
      481 atagcacaac tttttcggag aaccgcttgt tgaaaattga aacattgaaa agcattctta
      541 atcaataaca ttcaacaatt atcaagcgtg ccggttaccg gctgatcacc ttggtactca
      601 gcatacattg caggcattta gtgggctcgc agtacctgtt tttcaactga atcagggctt
      661 ggctgtcgaa cgcgttatgt gcctgtatcc caacttcgtt ccaacgcgag ataacactgt
      721 tgtgttcggc tggcaaggcc tcgagcaact cgagggcacg ctgtttcaac agggcaatat
      781 tgttgcgctc gccataaaga aacagaaatg gggctaccgt attaatgatg atgttgttgc
      841 gcgaagccat acccaagttt ttctcccctt ggggcgatgg caagctgaac cgataatgcg
      901 tatcccaata gggcgaagct ttcacgtcga aaaagtcgat gattttatcc aatgcgctct
      961 cttccaccag tttcgacaac aacgattcag acttgaacac caacgcagca aactgagcaa
     1021 tgcgaagggt tgggaagtta gcaggccgca aacgcataaa tttccagagg taaccttcca
     1081 tgcctttcaa cccatatttt cgggcaaaat aggaatattc ttcgcgcagg ttgagataat
     1141 aatcgtcgcc cagcaactct tcgttcaata atcccgactg tccgaaaagc aaagcctcga
     1201 gctggaacaa gttgtgtttg tgctttacta aaaccgacaa tggcgtgcag cgggccaaca
     1261 tctcgaaagg cagcccattg gtttt
//
LOCUS       DAOPUW010000633         1278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_123504_length_1278_cov_2.899428, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000633 DAOPUW010000000
VERSION     DAOPUW010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123504_length_1278_cov_2.899428"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..>1278
                     /locus_tag="PKJ43_09375"
     CDS             143..>1278
                     /locus_tag="PKJ43_09375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNZ72823.1"
                     /translation="MIHRNNIRSTNENHSEYGLRKLLQLALPVIASSFLSMAYSFINI
                     LFVGRLGSGAVAAVGTASFYMNLSWSISALITVGTGIKVSHAVGKKDLTLAKGYIRSG
                     LLAVGVIAFAYLVLLLIFNKELIGFVKLNDPAIEAASANYLVWAGLCIPFAFLNLFFT
                     SVFIGYGDSRSPFRINATAFALNILLDPILIFLVGLGIKGAAIATILSQVMATVLFFY
                     KVKATKTICPDRGAFSKKLLKDIIGLGVSPAVQRVSFTIVAIFMARIISDWGPAAIAV
                     QKVGVQIEAISYMTAGGFLSALSAMSGKAYGAKNYQGQWVAFKSGIKVAFVIGIVTSS
                     LFILFPEALFSIFINEEESIAMGAVYLKVLGFSQLFMCLEMVTTG"
BASE COUNT          342 a          238 c          297 g          401 t
ORIGIN      
        1 gaaaattaaa atcagaaaac tgtcccgaaa gagatattct atttaaaatc atcttattaa
       61 cacctataaa ccctgaataa aactattgag gcaggtattg tattatcctg ttgaagcgtt
      121 tagaaaacct tttaagtaaa taatgattca cagaaacaac atacggtcaa caaatgaaaa
      181 ccactcggaa tatgggttaa gaaagcttct gcaactggcc cttccagtaa ttgcatcctc
      241 gtttttgtcg atggcataca gtttcattaa tatcctgttt gttggaagac tgggaagcgg
      301 tgcggttgcc gcggttggaa ctgccagttt ttacatgaac ctaagctgga gcatctcggc
      361 actgattacg gtaggaaccg gaataaaagt atcgcacgct gtgggaaaaa aggatttgac
      421 gctggctaaa ggttatattc ggagtggatt gttggctgtt ggtgtgattg cttttgcgta
      481 cctggttttg ctgttgattt ttaataaaga actgattggc tttgtcaagc taaacgaccc
      541 agctatcgaa gcggcatcgg ccaattatct tgtgtgggca ggtttatgca tcccgtttgc
      601 ttttctgaac ctgtttttca ccagtgtttt tatcgggtat ggcgatagtc gttctccttt
      661 tcggatcaac gccacagcct ttgcattaaa tatcctgctt gatccaattt tgatctttct
      721 ggttggatta ggaattaaag gtgcggctat tgccacaatt ctttcgcaag tgatggcaac
      781 agtgctcttc ttttataaag taaaagcaac aaaaaccatt tgccccgata gaggggcttt
      841 ctcaaaaaaa ttactgaaag atatcatcgg gctgggtgta agtcctgccg tgcaacgggt
      901 ttcattcaca attgtggcca tttttatggc acgcatcatc agtgactggg ggcctgctgc
      961 tattgctgtt cagaaagttg gtgtccagat tgaagccata tcgtatatga cggctggtgg
     1021 ttttctgtcg gcactttcag caatgtcggg gaaagcttat ggtgcaaaaa attaccaggg
     1081 gcaatgggtt gcgttcaaat cgggaattaa agtggctttt gttatcggaa ttgttacatc
     1141 aagcctgttc atcttatttc cggaagcact tttttcaatc ttcattaacg aagaagagag
     1201 cattgcaatg ggagctgttt atttaaaggt gttgggattt tcgcaacttt tcatgtgcct
     1261 cgaaatggta actaccgg
//
LOCUS       DAOPUW010000634         1275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_123977_length_1275_cov_2.914754, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000634 DAOPUW010000000
VERSION     DAOPUW010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123977_length_1275_cov_2.914754"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="PKJ43_09380"
     CDS             <1..1123
                     /locus_tag="PKJ43_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72824.1"
                     /translation="ASSKDFSDFKAYAADAVKNDRKELKNLEISAYASPDGEQELNDK
                     LSQKREGSASELLKKELKQQEVEANIKTKYTAEDWDGFRKLLEASSVKDKELILRVLS
                     MYSDPEVREKEIKNLSEAFTAVAEQILPQLRRAKFSANIDLIGKTDAELLEFADNSPE
                     KLNQAELLEAARLTDDNAKKVKFYTSFTRQFPEDWRGFNNLGMAQVWTGDLDAAANNF
                     SKADKLSQGNPIIQNNLGVAALYKGETSKAIEYFSAASGAGKQVDYNMGIVSSTRAEY
                     DKAVKYFGDSNKPNAALAKILAGDNNGALKTLEACDKNCPMAAYLKAVVGARTAKENL
                     LLESLEAACKLDPKMKQAAKTDLEFAKYFQNPKFKSLVD"
BASE COUNT          417 a          276 c          267 g          315 t
ORIGIN      
        1 ggcttcaagc aaagacttct ccgatttcaa agcgtatgct gccgatgcag ttaaaaatga
       61 cagaaaagag ttgaaaaacc tcgaaatttc ggcgtatgca tcaccagatg gggaacaaga
      121 attgaacgat aaactttcgc agaaacgtga aggaagcgct tcagaacttc tgaaaaaaga
      181 gttgaaacag caagaagttg aagcgaacat caaaacaaaa tacactgctg aagactggga
      241 cggtttccgc aaattactcg aagcttcttc tgttaaagat aaagaattga tccttcgcgt
      301 tctttctatg tacagcgatc ctgaagttcg tgaaaaagaa atcaaaaacc tttctgaagc
      361 tttcaccgct gttgctgaac aaattttacc ccagttgcgt cgtgctaaat tctcggccaa
      421 tattgacctt attggaaaaa ccgatgctga attgttggaa tttgccgata acagccctga
      481 aaaactcaat caagctgaat tgctcgaagc tgcacgtttg accgacgaca atgcaaagaa
      541 agttaaattc tatacttcgt tcacccgtca gttccctgaa gactggagag gattcaacaa
      601 cctcggaatg gctcaggttt ggacaggcga tcttgatgct gctgccaata atttcagcaa
      661 ggctgacaaa cttagccagg gcaacccaat cattcagaac aaccttggtg ttgctgccct
      721 ttataaaggc gaaaccagca aggctattga atatttctca gctgcatcgg gtgcaggtaa
      781 acaagttgac tacaacatgg gtattgttag cagtacacgt gccgaatacg acaaagcagt
      841 gaaatacttt ggcgatagca acaaaccaaa tgctgccctt gcaaaaatac ttgccggcga
      901 caataacggt gcattgaaaa cccttgaagc ttgcgacaaa aactgcccaa tggctgcata
      961 tcttaaagct gttgtaggtg cccgcactgc aaaagaaaac ctcttgctcg aaagcctcga
     1021 agctgcatgc aaattggatc ctaaaatgaa acaagctgca aaaaccgacc tcgaatttgc
     1081 aaaatacttc caaaatccga agttcaaatc attggttgac taatgttgat aaaacataat
     1141 ttcaaaaggg tgtctgaaaa ggcacccttt tttgttgcat ctacccgatg acatttaatg
     1201 agctgatttg gtttattgtt tgaacgagca atatgtcaat gaccttgtgg cgtaagccca
     1261 cggttggcaa aaccc
//
LOCUS       DAOPUW010000635         1274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_124151_length_1274_cov_2.639869, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000635 DAOPUW010000000
VERSION     DAOPUW010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124151_length_1274_cov_2.639869"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PKJ43_09385"
     CDS             complement(<1..683)
                     /locus_tag="PKJ43_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNZ72825.1"
                     /translation="MRRLFMLSLSLFLFFSCSNQNENLRVVKNKALVPFAPPVSYSEA
                     QKKADSIVMSMTIDERIDMIGGHNMFFVQGVPKANLPRLYLSDATQGVHLRKDLDSQL
                     EKSTAMPCPITLTSTWNRDLAHIYAKSIGEECRAGEIAVLLGPGMNIYRIAQNGRNFE
                     YFGEDPYLAARMIENYVVGLQSTGTIATLKHFMLNNTDFRRRTSNSVVSERTMHEIYL
                     PAFKAGIDAG"
     gene            complement(773..>1274)
                     /locus_tag="PKJ43_09390"
     CDS             complement(773..>1274)
                     /locus_tag="PKJ43_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HNZ72826.1"
                     /translation="DSFSVAGNEHLNIIPISNPVQSESYKYGQTVLVGAPGCSLKQNQ
                     PPQFERAKLISLGDQSRLIISGTASIKGQETVGLGDVEEQTRTTIENIEALASPENLK
                     RQFPQLGAVPSRYSYIRVYVKHKKDFGKVRNICTDFYGQVPAMYVVADICRDNLLVEI
                     EAELIS"
BASE COUNT          384 a          274 c          250 g          366 t
ORIGIN      
        1 ccagcatcga taccagcttt aaaggccgga agatatattt cgtgcatagt gcgttcagaa
       61 actacagaat ttgatgtacg cctgcgaaaa tcggtattat taagcataaa atgtttaagt
      121 gtggcgatcg ttccagtgct ctgaagccct acaacatagt tttcaatcat tcgggctgcc
      181 aaatatgggt cttctccaaa atactcaaag tttcttccgt tctgggcaat tcggtaaatg
      241 ttcattcccg ggccaagaag aacagcaatt tctcctgcac ggcattcttc gccaatagac
      301 ttagcataaa tatgagcaag atccctgttc caagtagacg ttagagtaat aggacaaggc
      361 atggctgtcg atttttcgag ctgagaatca agatccttgc gcaaatgaac cccttgagtt
      421 gcgtcactaa ggtaaagtct gggcaaattg gctttgggaa ccccctgaac aaaaaacatg
      481 ttgtgaccac caatcatatc aatgcgttca tcgatagtca tcgacatcac aatagaatca
      541 gctttttttt gtgcttcgct atacgatacc ggaggtgcaa acggcacaag agccttgttc
      601 tttactaccc ttaggttttc gttctggtta gaacacgaaa agaacagaaa caaacttaga
      661 cttaacataa ataatcttct catatttctg gtatttaatt gtatttgccc gacaacttca
      721 tgactggaaa ttaatcacca gaaaaatagc aaaaagaaca atagaaaaaa agtcaggaaa
      781 tcaattcggc ttcaatctct accagcaaat tatcacgaca aatatcggca acaacataca
      841 tagcaggaac ttgaccataa aaatcggtac aaatattacg aaccttaccg aaatcttttt
      901 tgtgctttac atataccctt atgtaactgt agcggcttgg aacagctcca agttgaggaa
      961 actgacgttt gagattttcg gggcttgcca aggcttcaat attttcgata gtagtgcgcg
     1021 tttgttcttc aacatcgcca aggcctactg tttcttgccc ttttattgag gctgttcctg
     1081 aaataataag cctcgattgg tcaccaagcg aaatcaattt tgccctttca aattgcgggg
     1141 gttggttttg tttcaacgaa caacccggtg ccccaacaag tactgtttgt ccatatttat
     1201 atgattcgct ctgtacaggg ttgctgatcg ggataatatt caggtgctcg tttccggcaa
     1261 cagaaaaact atcg
//
LOCUS       DAOPUW010000636         1269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_124944_length_1269_cov_3.420923, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000636 DAOPUW010000000
VERSION     DAOPUW010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124944_length_1269_cov_3.420923"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>321
                     /locus_tag="PKJ43_09395"
     CDS             <1..>321
                     /locus_tag="PKJ43_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HNZ72827.1"
                     /translation="KEPLFEKEINARELWQKIVHNAWKSAEPGILFWDTIIKESLPDC
                     YADLGYRTVSTNPCGEIPLCPYDSCRLLAINLYSYVEQPFTKAARFNFDLFKEHIGMA
                     QRIM"
     assembly_gap    322..331
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <332..>1269
                     /locus_tag="PKJ43_09400"
     CDS             <332..>1269
                     /locus_tag="PKJ43_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HNZ72828.1"
                     /translation="DIIDLELEKIDGILKKIASDPENEEIKATEYNLWKNIRKKAKEG
                     RRTGVGITAEGDMLAALGLTYGSENAIDFSEEIHKTVTLEAYRSSVKMAQERGNFSIY
                     DANREKNNPFIHRIKEADPKLYEEMVRHGRRNIALLTIAPTGTTSLMTQTTSGIEPVF
                     LPVYKRRRKVNPNDKDVQVDFIDELGDSWEEYSVFHHKFLTWMEVNGYETNKRYSQEE
                     LDEMVKKSPYFQATSNDVDWLNKVRMQGRIQKWVDHSISVTINMPEDVTEDLVDKVYF
                     EAWKSGCKGVTVYRDGSRSGVLISDKKKKTEEEPAL"
BASE COUNT          450 a          253 c          262 g          294 t
ORIGIN      
        1 aaaagaacca ctatttgaaa aagagattaa tgcccgcgaa ttgtggcaaa aaattgtgca
       61 caatgcatgg aaatctgccg aacctggaat tttattctgg gatacgatca ttaaagagtc
      121 gttgcccgac tgttatgccg atttgggcta ccgcacggtg tcgacaaatc cctgcggaga
      181 aataccactt tgcccatacg acagttgcag gttgttggcc atcaacctct attcttatgt
      241 agaacaacct tttacaaaag ctgctcgctt caatttcgat ttattcaagg aacatatcgg
      301 aatggctcag cgaataatgg annnnnnnnn natgacatta ttgatcttga attagagaaa
      361 attgatggta tcctgaagaa aattgcaagc gatcctgaaa acgaagaaat aaaagcaaca
      421 gaatacaacc tctggaaaaa catacgtaaa aaagccaaag aaggacgacg caccggggtt
      481 ggcatcactg ctgaaggaga catgttagct gcattaggtt tgacctatgg atcagaaaat
      541 gcaattgatt tttcggaaga aatacacaaa accgttaccc tcgaagcata tcggtcatca
      601 gtaaaaatgg cacaagaacg tggaaatttt tcaatctacg atgcgaatcg agaaaagaac
      661 aacccattca tacacaggat aaaagaagcc gatcctaagc tttatgaaga aatggttcgt
      721 cacggccgtc gcaacattgc actgcttacc attgccccta ccgggacaac cagtctaatg
      781 acccaaacca cgtcaggaat cgaaccggta ttccttcctg tatacaaacg acgcagaaaa
      841 gtaaacccaa acgacaaaga tgttcaagta gatttcatcg atgaattagg cgacagctgg
      901 gaagaatata gcgtattcca tcataaattc cttacatgga tggaagtaaa cggatacgaa
      961 accaataaac gctattctca ggaagaactt gatgaaatgg taaaaaaatc gccctatttt
     1021 caagcaactt ctaacgatgt tgattggctt aataaagtac gcatgcaagg ccgaatacag
     1081 aaatgggtag accattctat aagtgtaacc atcaatatgc ccgaagatgt taccgaagac
     1141 ttagtggata aagtttattt tgaagcttgg aaatcaggct gcaaaggagt aacggtatac
     1201 agagacggct cacgttccgg agtattaatt tccgataaaa agaaaaaaac agaagaagaa
     1261 ccagccctg
//
LOCUS       DAOPUW010000637         1266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_125429_length_1266_cov_2.841453, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000637 DAOPUW010000000
VERSION     DAOPUW010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125429_length_1266_cov_2.841453"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1266)
                     /locus_tag="PKJ43_09405"
     CDS             complement(<1..>1266)
                     /locus_tag="PKJ43_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNZ72829.1"
                     /translation="NKAAREMKERISAIVGNDIARYLWMGTFHSIFSRILRTESDAIG
                     YPSTFTIYDTSDSKSLIRSIIKEMQLDPKTYKPGTVLNRISSAKNNLVTAQAYAANKE
                     VREYDRSIQMPLIADIYKEYAKRCKTSGAMDFDDLLLNMNLLLHRNPEVLQRYQERFR
                     YILVDEYQDTNYSQYLIVKKLAEKHKNICVVGDDAQSIYSFRGARIENILNFRNDYPG
                     FQTFKLEQNYRSTQTIVNAANSVIKKNKRQLHKEVFSENEPGAQIQIIQAITDNEEGI
                     LVATELNQHRLREHDQFSDYAILYRTNSQSRIFEEQLRRLNIPFKIYGGQSFYERKEI
                     KDLLAYLRVVVNCNDSEAVKRIINFPARGIGQTTIDKIEMAASTNDVTFWEIITKTDS
                     YTVGLNKGTLTKVEKFITLIQSFKEQLSTS"
BASE COUNT          345 a          269 c          235 g          417 t
ORIGIN      
        1 cgatgtggaa agttgctctt taaaagactg aatcaacgta atgaacttct caactttagt
       61 caacgttcct ttattgagac cgacagtgta actatcagtc ttagtgatta tttcccaaaa
      121 agtaacatcg tttgtggaag ccgccatttc aatcttgtca attgtagttt gcccaatgcc
      181 cctggccgga aaattaatta tccgtttaac agcttcacta tcgttacaat tgacgacaac
      241 tctcaagtag gccagtaaat ccttaatttc ctttcgctca taaaacgatt gcccaccata
      301 tattttgaaa ggaatattca gcctccgcaa ttgctcctcg aatattcgcg actgtgagtt
      361 tgttcgataa agaatcgcat aatcggaaaa ctgatcgtgc tcacgaagcc tatgctggtt
      421 gagttctgtg gcaacaagaa ttccttcctc gttgtcggta atagcctgaa taatttgtat
      481 ttgagcaccc ggttcattct ccgagaatac ttctttgtgt aactgccttt tattcttttt
      541 aataacactg tttgccgcat tcacaatggt ttgtgtcgac cggtaattct gttccagttt
      601 gaaggtttga aaaccaggat aatcgttcct gaaatttaaa atattctcaa tccttgcgcc
      661 acgaaatgaa tagatgcttt gtgcatcatc accaacaaca cagatatttt tatgtttttc
      721 tgcaagtttc tttacgataa ggtattgcga atagtttgta tcctgatatt catcaaccaa
      781 aatatacctg aatctttcct ggtatcgttg caatacctca gggtttcgat gaagcaagag
      841 attcatattc aacagtaaat catcgaaatc cattgccccc gatgttttgc atcgttttgc
      901 atattcttta taaatatcgg caatcagtgg catttgtatc gaacggtcgt attcgcgcac
      961 ttctttgttg gccgcataag cctgagcggt taccagatta tttttagccg atgaaatccg
     1021 gttaagcacg gttcctggtt tgtacgtttt cggatcgagc tgcatttctt ttataatgct
     1081 gcgaattaaa ctcttagagt cggaagtatc ataaatagta aacgtcgacg gataaccaat
     1141 tgcatcagat tcggtgcgta atattcgtga gaagattgaa tggaacgttc ccatccacaa
     1201 atagcgggca atatcgttcc ccactatagc cgagatacgt tctttcattt ccctggccgc
     1261 tttatt
//
LOCUS       DAOPUW010000638         1266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_125450_length_1266_cov_2.459125, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000638 DAOPUW010000000
VERSION     DAOPUW010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125450_length_1266_cov_2.459125"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PKJ43_09410"
     CDS             <1..648
                     /locus_tag="PKJ43_09410"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF012991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNZ72830.1"
                     /translation="MGALERLNRVGIRKLVGIHRGFSPFQETKYRNYPGWKTYIELRR
                     SMPNLPIICDPSHIAGRREYLYEISQKAFDMGFDGLIIESHIDPSCALSDKEQQLTPA
                     DLSKLLDKLVIRAEHSTNTEYETQLDLLRSRIDSLDTELIELLAARNEIVKQIAMYKK
                     NNNVMALQIDRWTKMMNNRVAAASKMELDTVFIKILFQLIHEDSVRMQTELIDNE"
     gene            complement(735..>1266)
                     /locus_tag="PKJ43_09415"
     CDS             complement(735..>1266)
                     /locus_tag="PKJ43_09415"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HNZ72831.1"
                     /translation="SHLIVTGKITDSDLMEKPGLHRSLLGDGISNVLSGFAGSPPNTT
                     YGENIGVMAITRVYSVWVIRGAALLAIAFSVIGKVSAAISTIPDAVMGGITMLLYGVI
                     AVQGFRMFVEQKVDFSKNSNMVLGAVVFVIGVSGASINIGSVQLKGMAFAAIVGVVLS
                     LVFWLFRKFKIMDKDV"
BASE COUNT          389 a          286 c          249 g          342 t
ORIGIN      
        1 atgggggctc ttgaacgcct gaaccgggtg ggaattagaa aattggtagg aatacaccgt
       61 ggattttctc cttttcagga aacaaaatac cgcaactatc ccggttggaa aacctatatt
      121 gaacttagac gttcaatgcc caacttgcca ataatttgcg acccaagcca cattgctggt
      181 cgtcgtgaat acctttacga gatttctcaa aaagcatttg atatgggttt cgacggcttg
      241 attatagaat ctcatatcga tccatcatgc gcattaagtg ataaggaaca gcaacttact
      301 ccagctgatt tgtccaaatt gctcgacaag ctcgttattc gtgccgaaca ttcaacaaat
      361 acagaatacg aaacgcaact tgatcttttg cgtagccgaa ttgactcact cgataccgag
      421 ctgattgaat tgcttgctgc ccgtaacgaa attgtgaaac aaattgcgat gtacaagaaa
      481 aacaataatg ttatggcgct ccaaatcgat cgttggacca agatgatgaa caaccgtgta
      541 gctgctgctt ccaaaatgga acttgatact gtttttataa aaatactttt tcaacttatc
      601 cacgaagatt cggtacgtat gcagaccgag ttgattgata acgaataaat ttaaaaaagg
      661 ctgtcaggtt gtaatctggc agcctttttt catggaaata attttgtacg gttgggcaaa
      721 tcttgttgtt gctgttacac gtctttatcc attattttga atttccggaa aagccagaaa
      781 acaagcgaaa gcacaacgcc tacaatagcc gcaaaggcca tcccctttaa ctgcaccgaa
      841 ccaatgttga tacttgcacc actaacgcct ataacaaaaa ccaccgcacc aagcaccata
      901 ttgctgtttt tgctgaaatc gactttttgt tctacaaaca ttctgaaacc ttgtactgca
      961 attactccat aaagcaacat ggtaatgccg cccatcaccg catcggggat cgtagaaata
     1021 gcagccgata cttttcctat tacagaaaaa gcgatggcca gtaaggcagc gccccggata
     1081 acccataccg aatatacacg ggtaatggcc attacgccaa tgttttcacc atacgttgta
     1141 ttgggaggtg agccagcaaa acccgataat acattgctaa taccatcgcc aagtaacgat
     1201 cggtgtaagc ctggtttttc catcaggtca gagtcggtaa tcttgcctgt aacgatcaaa
     1261 tggctt
//
LOCUS       DAOPUW010000639         1262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_126035_length_1262_cov_2.859155, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000639 DAOPUW010000000
VERSION     DAOPUW010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126035_length_1262_cov_2.859155"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /gene="sprA"
                     /locus_tag="PKJ43_09420"
     CDS             complement(<1..1248)
                     /gene="sprA"
                     /locus_tag="PKJ43_09420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNZ72832.1"
                     /translation="MEDINRDNTMNETEAYYQYRVSMRPEDLEVGRNHIVDKITVVPS
                     NLPNGSKDPVTWYQFKVPVKEYDEKIGSISDFKSIRFIRMFLTDFSDSVTLRFATLAF
                     VKSEWIKEINDLAQDGTVSNPETKFDMTSVNIEENGSKKPINYILPPGIDREVDPTST
                     SMVKMNEQSMLLKVQELAPGDMKAVYKSAGIDMRRNKKLKMEVHAEAIEDSPLADYEV
                     SLVVRLGSDYDNYYEYEIPLKVTPESPTSYNGDKLYSADRYIVWPEENRLDVNLDIFP
                     QLKEKRDAAMRKAGSTVTNRTIYEMVDSDSPKGKNIIRVKGNPSIGEVEIIHIGIRNP
                     KGKNTQNKSVEVWVNELRMSDVEEKGGWASNARVNLRLADLGTVSLAGSTQSVGWGSI
                     NETSATRNLEDRYQYDFAANIELG"
BASE COUNT          302 a          287 c          247 g          426 t
ORIGIN      
        1 tcccaattcg atatttgcag caaagtcgta ttgataacga tcttccagat tgcgggtcgc
       61 cgatgtttcg ttaatgcttc cccaccctac cgattgtgtt gaaccagcca atgaaacggt
      121 accaagatcg gcaagtctga ggtttacccg ggcgttggat gcccatccgc ctttctcttc
      181 aacatcagac atacgtaact cattaaccca cacttcaact gatttatttt gggtgttttt
      241 ccccttagga ttacgtattc caatgtgtat tatttcgact tcaccaatgc tcggatttcc
      301 tttcacccta atgatgtttt tccctttagg tgaatcgcta tcgaccattt cgtatattgt
      361 cctgttcgtt acagttgaac cagctttgcg cattgctgca tcgcgttttt ctttcaactg
      421 tgggaaaatg tctaaattaa cgtccaacct gttttcttca ggccaaacaa tatatctatc
      481 ggctgagtat agcttgtctc cgttatacga tgttgggctt tcgggggtaa cttttaatgg
      541 aatttcatat tcgtaatagt tatcgtagtc gctacccaag cgaacgacca atgatacttc
      601 gtaatcggcc aatggagaat cttcaattgc ttcggcatgg acttccatct tcagcttttt
      661 gttgcggcgc atatcaattc ctgcactctt gtagacagct ttcatatcgc caggagcaag
      721 ttcctgtact ttcaacagca tcgactgttc attcattttc accatcgatg tgctggttgg
      781 gtcaacttcg cgatcgattc ccggaggcaa aatataattt attggctttt tacttccatt
      841 ctcttctata ttgaccgagg tcatatcgaa cttcgtttca gggtttgaaa cggttccgtc
      901 ttgtgcaaga tcgttaattt cctttatcca ttccgatttt acaaaagcca gtgtagcaaa
      961 ccgaagagta accgaatcgg agaaatcagt aaggaacatt cgtataaatc gaatcgattt
     1021 aaagtcggaa atacttccta ttttttcgtc atattcttta acgggaactt taaattggta
     1081 ccaggtaaca gggtctttac taccattggg taaattagat ggaacaacag tgattttgtc
     1141 tactatatga tttcggccaa cttctaagtc ttcggggcgc atgctcaccc ggtattgata
     1201 gtacgcctct gtttcgttca ttgtgttatc ccggtttata tcttcgatgt cgggttcggc
     1261 cg
//
LOCUS       DAOPUW010000640         1259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_126541_length_1259_cov_2.770764, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000640 DAOPUW010000000
VERSION     DAOPUW010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126541_length_1259_cov_2.770764"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PKJ43_09425"
     CDS             <1..761
                     /locus_tag="PKJ43_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HNZ72833.1"
                     /translation="LAMMNVIQIEKGKERWSMIADNIWDMLMGKGGKLPGKLAPEIVK
                     LAQENGKTEFSDNPQSLYPDALDKFRKEMNDNGWDFGPDDEELFELAMHPEQYRLYKS
                     GAAKKAFEEDLAKKKNEGGGLIATKANVADVAFAPTTLNVNVNGEVFKVTVSYGDETG
                     KQSEANPVAIPIASGEEKKILSPLEGKFYLTKNTTEKPLQVGDKVEVGDLIGYIEAMK
                     TMNSIRADKAGTVVKIEKSIGQDVEEDDVLVVIQ"
     gene            809..>1259
                     /locus_tag="PKJ43_09430"
     CDS             809..>1259
                     /locus_tag="PKJ43_09430"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNZ72834.1"
                     /translation="MDVLQKLYEMTAFGSLIENPGSLLMLAIGSILLYLGIRKQYEPL
                     LLVPIAFGVILANLPGGGMGVVPGEFIQLEGLKQAASPDMNLMELEMEHRYMNLFEIA
                     HEYGIMNFLYYMLIKTGLLPPIIFMGVGALTDFGPMLRNLRLSFFGAA"
BASE COUNT          393 a          217 c          299 g          350 t
ORIGIN      
        1 acctggcaat gatgaatgtt attcagattg aaaaaggtaa ggaacgctgg agcatgattg
       61 ccgataatat atgggatatg ttaatgggca aggggggaaa attgccagga aaacttgctc
      121 ccgaaattgt aaaattggct caggaaaacg gaaaaactga attctcagac aatccacagt
      181 cattgtaccc cgatgcactt gataaattcc gtaaggaaat gaacgataac ggatgggatt
      241 ttggccccga cgacgaagaa ctcttcgagt tagccatgca cccggaacaa taccgtttgt
      301 ataaatcggg agcagctaaa aaagcgttcg aagaagatct tgctaaaaag aaaaacgaag
      361 gtggaggact tattgccact aaagcgaatg ttgctgatgt tgcatttgct cctactacac
      421 tcaatgtaaa tgttaatgga gaagtattca aagtaacagt ttcgtacgga gatgaaacag
      481 gcaaacaaag cgaagccaat cctgtggcaa taccaatagc ttccggcgaa gagaagaaaa
      541 ttctttcacc tctcgaagga aagttttatt tgacaaagaa tacaacagag aagcctttac
      601 aagttggcga taaagtagaa gttggtgatt taattgggta tattgaagca atgaagacta
      661 tgaattctat ccgtgccgat aaagctggaa ctgttgttaa gatagagaaa tcgataggtc
      721 aggatgttga agaagacgat gttttggtag taattcaata aaactgttag cgaaaccaat
      781 ttcgccatgg tttttcaaaa ctttagttat ggacgtttta caaaaattat atgaaatgac
      841 tgctttcggc agtcttatcg agaatccggg atcgttactg atgctcgcta ttggatcgat
      901 attgctatat ctcggaatca gaaagcaata cgaacccctt ttgttagtgc cgattgcttt
      961 tggcgttatt ttggccaatc tgcccggagg cggaatgggc gttgtcccag gtgaattcat
     1021 ccagctcgag ggattgaagc aagccgcgag ccccgatatg aatctaatgg agcttgaaat
     1081 ggagcatcgt tatatgaacc ttttcgaaat cgctcatgaa tacggaatta tgaattttct
     1141 gtattatatg ctgataaaaa ctggtttact tcctcctatt atttttatgg gagttggtgc
     1201 tttgaccgat tttgggccga tgttgcgcaa tttgcgattg tcgtttttcg gagcagctg
//
LOCUS       DAOPUW010000641         1257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_126915_length_1257_cov_2.304493, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000641 DAOPUW010000000
VERSION     DAOPUW010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126915_length_1257_cov_2.304493"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..698)
                     /locus_tag="PKJ43_09435"
                     /pseudo
     CDS             complement(30..698)
                     /locus_tag="PKJ43_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
     gene            1039..>1257
                     /locus_tag="PKJ43_09440"
     CDS             1039..>1257
                     /locus_tag="PKJ43_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNZ72835.1"
                     /translation="MKSTDYEYKYKKGIEYYEAGDYNHAITLFEDLVYAFRGTSKGDD
                     LYYYYASSCFKAEDYTLAGYYYKSINEQY"
BASE COUNT          374 a          278 c          229 g          376 t
ORIGIN      
        1 tataccggaa gtgattgcaa atttcttcat cacaagcgat aaaagcacca accagccatc
       61 gctttggcaa atgttgaaaa atgaaggtcg atttgatctt gcaccccaaa atactcagga
      121 gttccggcac cggttttccc catggtacca aagccgtgag catcgtcgac caaaagcctg
      181 aaattgaaat ttttctttcg ttcaacaatc tcatccaact ttccaaggtc gccagccata
      241 ccgaaaacac cctcggtaat aacaaggatc cctccgccag ttttttcaac cagacgggta
      301 gcatgcatca actgctagtc gaggctttcc atgttattgt gctggaaaac aaaacgtttt
      361 ccggtatgga ggcgcaaccc atccataatg caagcatgcg attcagcatc gtatacaatt
      421 acatcgttac ggtcaactaa agaatcaatg atggaaatca tgccctgata accaaagttc
      481 aaaagaaacg catcgggttt tccaacaaaa gcagcaagtt gttgttctag ttcttcgtgc
      541 ttgctggttt gccccgacat catccgggca cccatcggat aggccatacc aaactctgcc
      601 gcggccttgg catccgcctc ccgaatttcg ggatgattgg ccaggccaat gtaattattc
      661 aaactccagt ttagaacttc ttttccccta aatttcattc gaggtcctat ttcaccttcc
      721 aatttaggga acataaaata accatgtgct tgcttctggt attggcccaa ggctccttta
      781 tccgatttaa gtttctcaaa tatgtccatc attttttgtt tgaatcaatc gtgcaaaagt
      841 aaaatttttt atgctgtttg caaattgtgt taaataacgc taaataaatt tttgatgtat
      901 cataaaactt taattttcag gtttcaattt taaaagtaaa agtatacttt tgcacaacat
      961 tttttactat gaaaataaag tggtttttct tattggcttt tagctcgtta cttttctcgc
     1021 ggtgaattta acactgtttt gaagagtacc gactacgaat ataagtataa aaaagggatt
     1081 gaatactacg aagcgggcga ttataaccac gctattacgc tgttcgaaga ccttgtttat
     1141 gccttcaggg gtacatcgaa aggcgacgac ttatattact attatgcaag cagttgcttc
     1201 aaagcagagg attacaccct tgccggatac tattacaaaa gtattaatga gcaatac
//
LOCUS       DAOPUW010000642         1253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_127579_length_1253_cov_1.722871, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000642 DAOPUW010000000
VERSION     DAOPUW010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127579_length_1253_cov_1.722871"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PKJ43_09445"
     CDS             complement(<1..225)
                     /locus_tag="PKJ43_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNZ72836.1"
                     /translation="MRSLIGKQAPKFSAAAVVNGHEIVDNFSLEQYKGNKYVVFFFYP
                     MDFTFVCPTELHAFQEKLAEFEKRNVAVVGC"
     gene            405..>1253
                     /locus_tag="PKJ43_09450"
     CDS             405..>1253
                     /locus_tag="PKJ43_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HNZ72837.1"
                     /translation="MKKFVSLFVLILLSASAWCQSHPNYTVIVSLDGFRWDYPTLYST
                     PHLDQIAARGSSATMLPSYPASTFPNHYTLATGLIPDHHGIVNNTFWDTQNNRQYSMN
                     NPETRFNPAYYLGEPIWVTAQKQGVKTANMYWVAADIPIKNTFPTYQKLFRQKPQLTF
                     EERIDTVISWLRMPEEARPQLVMLYFEEPDGTGHHFGPRSLETKRKVEYLDSLVGVLM
                     HKISRLPIADRINLIVTSDHGMTEISDERVVNMHPYLKPEWCRVIDGKTPTSVFTKPE
                     FRDSVYN"
BASE COUNT          371 a          279 c          260 g          343 t
ORIGIN      
        1 acagccaact actgccacat ttcttttttc aaattcggcc agtttttcct gaaaagcgtg
       61 gagttccgtg ggacaaacaa atgtaaagtc catcggatag aagaaaaata ccacgtattt
      121 gttacctttg tattgttcca aagaaaagtt atcgactatt tcgtgtccgt taactactgc
      181 tgcagcacta aatttaggtg cttgttttcc aattaatgat ctcataatgt attttatttt
      241 tagaagttat ttaatccttt ttctgaaagc aaaactacgg taatctgcag aggtggcaaa
      301 ataaatacaa tggataaaat ctatggactt atagatgaaa tctatcaggg gaaacaatta
      361 cttttgcaca tccgttcgta gtcactaaac agaaagtatg taaaatgaaa aagttcgtca
      421 gtttattcgt tctaattctg ctatccgcat cagcgtggtg ccaatcgcac cccaactaca
      481 ccgttatcgt atcgctcgat ggttttcgct gggattaccc taccttgtat agcactcccc
      541 atttagacca gatagccgcc agaggttcgt cggccaccat gcttccctcc tatccggctt
      601 ccacttttcc gaaccattac acgctggcta ccggactgat tcctgatcat catggcatag
      661 taaacaatac attttgggat acgcagaaca accggcaata ttccatgaat aatccggaaa
      721 cacgttttaa tccggcatat tatttgggcg aaccgatatg ggttacggca caaaagcagg
      781 gtgttaaaac ggccaatatg tattgggtgg cagccgatat acccatcaaa aatacatttc
      841 ctacttatca aaaattgttc aggcagaagc cgcagctaac gttcgaggaa cgcattgata
      901 ctgttatttc gtggttacgc atgcccgaag aggcacgccc gcaactcgtc atgctctatt
      961 ttgaagagcc cgacggcaca gggcatcact tcggtccgcg aagcttggaa acaaaaagaa
     1021 aagttgaata cctggatagt ttggtgggtg tgctgatgca caagatcagt aggctaccta
     1081 tagcggacag gatcaattta attgtaactt cggaccacgg gatgactgaa atcagcgatg
     1141 agcgtgtggt caacatgcac ccatatctca aaccggaatg gtgccgagtt atcgatggca
     1201 aaacgccaac atcggtattt acaaagcccg aattcagaga ttcggtgtac aat
//
LOCUS       DAOPUW010000643         1248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_128356_length_1248_cov_1.977368, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000643 DAOPUW010000000
VERSION     DAOPUW010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128356_length_1248_cov_1.977368"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="PKJ43_09455"
     CDS             complement(<1..1210)
                     /locus_tag="PKJ43_09455"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72838.1"
                     /translation="MDIFFSLARIVCDLCEWDMRTEIEKKFIENVKSIIKKGNSYPFR
                     VFNFNYFDIPIDLQLEASKQLARFFSKQVEAIKKRVSYSHNWPQHKRIRIGYISPDFR
                     SHPIGQVFYHMFQYHNRSEFEIYAYALFYQPNGEDIFRDKIVADVDHFSNISKLSYEE
                     AASQINTDEIDILIDLAGYTTYSKPQILALRPSPVQILFMGQPDSSGADFIDYYIADK
                     ILVPEENQQYYTEKIIYLPTGYFGSPLKISDKKYSKSDFGIPENAFVFCCFCNPYKYE
                     PVLFAAWMEILRQVENGVLWLNLAKSNRFKENILRYAKEHGIDASRIFFAERLAHPDY
                     MARYQICDLFLDTLHYSAGSTAISALMAGVPVITTTGTRNAENMGASICKAADLTETI
                     CNTSEEYIECA"
BASE COUNT          346 a          237 c          271 g          394 t
ORIGIN      
        1 cagcacattc aatatactct tcgcttgtgt tgcaaatcgt ttctgttaaa tcagctgcct
       61 tgcaaatgct tgcgcccata ttttcggcgt ttcttgtccc tgtcgttgta ataacaggga
      121 cgccagccat taatgccgaa attgccgtag aacctgcact gtagtgaagt gtatctagga
      181 ataggtcgca aatttggtag cgtgccatgt aatcggggtg tgcgaggcgt tcggcaaaga
      241 aaatgcgcga tgcgtcgatt ccatgttctt ttgcatatcg cagtatgttt tccttgaaac
      301 ggttactttt tgccaggttt agccaaagaa ctccgttttc aacttgcctg agtatttcca
      361 tccatgcagc aaaaagcact ggttcatact tgtaggggtt acagaaacag caaaacacaa
      421 aagcgttttc ggggattcca aagtcacttt ttgaatattt tttatccgat atttttaatg
      481 gagaaccaaa gtaacctgtt ggcagataga tgattttctc ggtataatat tgttggtttt
      541 cttcggggac taaaatttta tcggctatgt aataatcaat gaagtcggca ccactcgagt
      601 caggttgccc catgaaaagg atttgtaccg gcgatggcct taatgccaga atttgcggct
      661 ttgaatacgt ggtgtaccca gcaagatcaa tcaatatatc gatctcatcg gtattgattt
      721 gtgaagcagc ttcctcgtag ctaagtttcg atatgtttga aaaatgatcg acatcggcaa
      781 caattttgtc tcggaaaata tcttcgccat tcggttggta aaaaagggcg taagcgtaaa
      841 tctcaaattc gctccgattg tggtactgga acatgtggta aaatacctgt ccaatagggt
      901 ggctacggaa atcgggcgaa atgtaaccaa ttctgattcg cttgtgctgt ggccagttgt
      961 gagaatatga aactcgtttt ttgatggctt caacttgttt tgaaaaaaag cgggcaagtt
     1021 gtttcgacgc ttctagttga agatcaattg gaatgtcgaa gtagttaaag ttgaacaccc
     1081 taaatggata actattccct tttttaataa ttgattttac gttttcgatg aacttctttt
     1141 caatttctgt tcgcatgtcc cattcgcata aatcgcaaac tatgcgggca agcgaaaaga
     1201 aaatatccat attttatgtc tcatagagca gattcgaaca tatataaa
//
LOCUS       DAOPUW010000644         1248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_128357_length_1248_cov_1.966471, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000644 DAOPUW010000000
VERSION     DAOPUW010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128357_length_1248_cov_1.966471"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PKJ43_09460"
     CDS             complement(<1..744)
                     /locus_tag="PKJ43_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010421511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HNZ72839.1"
                     /translation="MRILKEKFSLYDAPQKVIDAGIYPYFREIESDQDTVVDINGKKV
                     LMFGSNSYLGLTNHPKIKEAAINAIKKYGTGCAGSRFLNGTLDIHIELENRLAELVGK
                     DAALCYSTGFQVNLGVVSVLAGRNDYLLLDELDHASIIEGSRLSFGKVMKFAHNDMES
                     LEAKLKLCEPDRMKIIVVDGIFSMEGDITKLPQIVQLAEKYGASIMVDDAHSLGVIGK
                     NGRGTASHFGLTDSVDLIMGTFSKSMASLG"
BASE COUNT          364 a          239 c          239 g          406 t
ORIGIN      
        1 gccaagtgaa gccatcgact ttgaaaaggt ccccatgatg aggtctacgc tatcagtcaa
       61 accaaagtga gacgcggttc cccttccgtt tttcccgatt acccccagag agtgggcatc
      121 gtcgaccatt atggatgcac catatttttc agcaagctga acaatttgag gcagtttagt
      181 aatgtcgccc tccatggaaa aaattccgtc aacaacgata attttcatgc gatcgggttc
      241 acaaagttta agctttgctt cgagagactc catgtcatta tgggcaaatt tcattacttt
      301 gccaaacgag aggcgactac cttcgatgat agatgcatga tcgagctcgt ctaataataa
      361 atagtcgtta cgacctgcca atacggagac aacgccaaga ttaacttgaa aaccagtact
      421 gtaacatagg gctgcatctt ttccgaccaa ttcggccaac ctgttttcaa gctcaatatg
      481 aatatcgagt gtaccattca gaaaccttga accggcacaa ccggtaccgt atttcttaat
      541 ggcattaatg gctgcttcct taattttggg atgatttgtt aacccgagat aactattcga
      601 accgaacatc aatacctttt taccattgat atcgactacc gtgtcctggt ctgattcgat
      661 ttccctgaaa tagggataaa ttcctgcatc gattaccttt tggggcgcat catacagcga
      721 aaatttttct tttaaaattc tcattggact atcagattag ttagactaac aggcggtaaa
      781 agtaaataaa aaagtaaaat ttggtactca tttgcttatg atttaacatt tgtcggctct
      841 tcttgcttgt attttaatgg tttatattgc ttgaactttc cttgaatttg tggtaaaact
      901 gccaatattc ttcgtatttg atcctcgcat tggcgatctt gaatgccttt ttttctcatt
      961 ccgtgttcca gagagcgata gttgttggct gttctaggaa attgatgttt tggatcgtat
     1021 ttcttttgct tattactatg taaattgcag aatgttaaat atttatattg gcatacaagt
     1081 tcaaggcatt tcaaaattgg atgagtttga gtatttttgc aggaaaggag aaaagtacaa
     1141 tgaagacgag tacagttata agtataccaa aatcgaaaat gtaaataata ttatagtgtt
     1201 ttcttgttgt aaaggccagg aaactggcct ttactttttt aaaaatac
//
LOCUS       DAOPUW010000645         1246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_128622_length_1246_cov_2.835432, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000645 DAOPUW010000000
VERSION     DAOPUW010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128622_length_1246_cov_2.835432"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1246)
                     /locus_tag="PKJ43_09465"
     CDS             complement(<1..>1246)
                     /locus_tag="PKJ43_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNZ72840.1"
                     /translation="VPVFSPDGERVAFVRDNNLFIKTIKFGTEFQITTDGKKNEIING
                     IPDWVQEEEFGYARAFEWSPDSKMIAFLKFNESDVPFFTMPVFKGLAPERNEGKLYTS
                     YDSLKYPKAGENNAIVTVHVYDVKVKSIIRVKTGDDPNVYFPRIQWNNSGSDLAIIKL
                     NRRQNELELLYANPFSGDTRSMMREKNNRYIDELFHKQFAFLPDNQHFVVVSERDGWS
                     HLYLYQNSGFLVRQLTTGNFDVTDFYGYDPVKQLFYYQAARKSPLQREVYALSFDGKK
                     ETLLSSQQGTNKAIFSNGFQYYLNYFSSAKTPLQVSVCDAKGKELRVIEDNAALRKKM
                     EGYQLPSFEFFTVKNSDGIELNGYMIKPSYFDAGKKYPVIMTQYAGPNSQEVTDSWQF
                     DWHNYLAERGFIVACVDPRGTAA"
BASE COUNT          350 a          291 c          263 g          342 t
ORIGIN      
        1 gcagctgtcc cgcgcggatc aacacacgca acaatgaaac cgcgttcggc cagataattg
       61 tgccagtcga actgccagct atcagtaact tcctgcgaat ttggccctgc atattgagtc
      121 ataataaccg ggtatttttt ccccgcatcg aaatacgaag gttttatcat atacccattt
      181 agctcgatcc catcgctatt ttttacggta aagaattcga aagaaggaag ctggtagccc
      241 tccatttttt tcctgagagc ggcattatct tcaataaccc gaagctcctt cccttttgca
      301 tcgcaaaccg acacctggag cggggttttt gccgacgaga aataattgag ataatattga
      361 aaaccattac tgaaaattgc cttgttggtt ccttgctgag aagaaagcag ggtttctttt
      421 ttgccgtcaa aagaaagtgc atacacttcg cgctgcaacg gcgatttgcg ggcagcttgg
      481 taataaaaaa gttgcttcac cggatcgtac ccgtaaaaat cggtcacatc gaaattgcct
      541 gtagttagct gccgcaccaa aaaaccactg ttttggtaaa ggtaaagatg cgaccagcca
      601 tcgcgctcgc tcactacaac aaaatgctga ttatcgggaa gaaaggcgaa ctgcttatga
      661 aatagttcat cgatatagcg attgttcttt tcgcgcatca tagaacgggt atcgcccgag
      721 aacggattgg catacaataa ttccaattca ttctgacgac ggttgagttt aatgattgcc
      781 aaatcggaac ccgaattatt ccattgaatc cttggaaaat acacattggg atcgtcgccg
      841 gttttcaccc tgatgattga tttcaccttt acatcatata catgcaccgt tacaatggca
      901 ttgttttcgc cagctttggg atatttcaac gaatcgtaac tggtgtaaag ttttccctcg
      961 ttccgttcgg gggcaagccc tttaaaaaca ggcatggtaa aaaagggcac atcgctttcg
     1021 ttgaatttca ggaaagcaat cattttgcta tcgggcgacc attcaaaagc ccgcgcatag
     1081 ccaaactctt cttcctgaac ccaatcaggt atcccgttaa taatctcgtt tttctttccg
     1141 tcggtagtaa tctggaattc agtgccaaac ttgatggtct taatgaaaag gttattgtcg
     1201 cgaacaaagg caacacgttc accatcgggg gaaaacacag gaacct
//
LOCUS       DAOPUW010000646         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_129104_length_1243_cov_2.556397, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000646 DAOPUW010000000
VERSION     DAOPUW010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129104_length_1243_cov_2.556397"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PKJ43_09470"
     CDS             <1..210
                     /locus_tag="PKJ43_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNZ72841.1"
                     /translation="MAQADKYFDTFFDYFEIIAERPFAFESVDYIKAGYRRCPCGTDA
                     IYYRINDNMVEIMAIVGMQDLKNIL"
     gene            complement(413..>1243)
                     /locus_tag="PKJ43_09475"
     CDS             complement(413..>1243)
                     /locus_tag="PKJ43_09475"
                     /inference="COORDINATES: protein motif:HMM:NF020249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HNZ72842.1"
                     /translation="QWRALSPLMCENYSIASDDMYFSILPTATMYARNSIFAGLMPAD
                     IKKIHPNCWDDDDNEEGGKNNFEEELLTKQLIRNGRKEKLYFEKMTTAKQGLKSNESI
                     SQIINSDLSVMVINSIDMISHARTEMNMIKELAGNEAAYRALSVNWFQHSGIMELLKE
                     LSSKNIEIIVTTDHGTIKVNNAVKVIGEKSTNSNLRYKLGRNLNYNPKEVFEIRKPED
                     ARLPSPNLSSSYIFAMNQDFFVYPNNLNYYANFYKNSFQHGGISLEEMIIPVIQLHPR
                     "
BASE COUNT          315 a          241 c          273 g          414 t
ORIGIN      
        1 atggctcaag ctgataaata ttttgatact ttttttgatt actttgaaat tattgcagaa
       61 aggccatttg catttgaatc tgttgattat ataaaagcag gatatagaag atgtccttgt
      121 ggcactgacg ccatatatta tagaatcaat gataatatgg ttgagataat ggcaattgtt
      181 ggaatgcagg atttgaaaaa tatactttaa tccctcatgt ccataattat tcacagatta
      241 gaaagtactg ttggcacaca cgcaaaataa cctgtggcgg gttcagtgct ggcttgaaag
      301 cttcttcccc acgccaggtt ttgtaacggt taacagggat gtggcccgct atccgccact
      361 ggtaatattg caaccgttgt atcaactaaa acaatgaatt cattgctgaa gtttaccggg
      421 ggtgaagctg aataaccgga atgatcattt cttcgagcga gataccccca tgttgaaacg
      481 aatttttgta aaagttggca tagtaattca aattattggg gtaaacaaag aaatcctggt
      541 tcattgcaaa aatatacgac gaagaaaggt tgggcgatgg caggcgtgca tcctcaggct
      601 ttcttatttc gaatacttct ttaggattgt agttcagatt ccggccaagt ttgtagcgca
      661 aattcgagtt ggtcgatttt tccccgatta ctttaaccgc gttatttact tttatggttc
      721 cgtgatcggt ggtgacaatt atctctatat ttttactgct tagttctttg agtaattcca
      781 ttattcccga gtgctgaaac cagtttaccg agagtgccct gtacgctgct tcgtttccgg
      841 ccagttcttt tatcatgttc atctcggtac gggcatgcga aatcatatca attgagttga
      901 ttaccataac cgacaagtct gaattaatga tttggctgat gctttcgttg cttttcagac
      961 cttgtttcgc cgtggtcatt ttttcgaaat agagcttttc ttttcgcccg ttcctgatga
     1021 gttgcttcgt cagcaattct tcttcaaagt tgtttttgcc gccttcttcg ttatcgtcgt
     1081 cgtcccagca attggggtgt atttttttga tgtcggcggg cattaagcct gcaaaaatgc
     1141 tgttgcgtgc atacatggta gccgttggca aaatgctgaa atacatgtcg tcgctggcaa
     1201 tgctgtaatt ttcgcacatg agcgggctaa gcgcgcgcca ctg
//
LOCUS       DAOPUW010000647         1241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_129402_length_1241_cov_2.991568, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000647 DAOPUW010000000
VERSION     DAOPUW010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129402_length_1241_cov_2.991568"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PKJ43_09480"
     CDS             complement(<1..453)
                     /locus_tag="PKJ43_09480"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ72843.1"
                     /translation="MLRYLLEKEIKQFVRNSFLPKMIVMLPLMAMLIFPLIANFEIKN
                     INLCVVDHDRSIQSQKLIQKIQSSGYFRITDVAESYQRAMLQVESNNSDMLLEIPVAF
                     GKDIEQGTSTNVLIAANAVNGVKGGLGSSYLTAILQNFSVDIATKIQLT"
     gene            complement(531..>1241)
                     /locus_tag="PKJ43_09485"
     CDS             complement(531..>1241)
                     /locus_tag="PKJ43_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ72844.1"
                     /translation="TYASSLISLYAKELSKEDEKALLISPQVKLLFNPSMKNSFNTVP
                     GIMGVIIILICALMTSVSLTREKEHGTMEVLLVSPMRPFLIIFSKMIPYFCISLVNLS
                     TILLLSHYMLGVPIVGSFGLVVLISLIYIFVSLALGLFISTVAEKQIVALLISGAGLM
                     LPVVYLSGMMFPLESMPVALQWFANIIPAKWFIIAMRNVMIKGLGITSVLREIIILVG
                     MGSLIVFASIKRFKIRLE"
BASE COUNT          382 a          280 c          243 g          336 t
ORIGIN      
        1 cgttagttgg atttttgttg caatatcaac actaaagttt tgcagtatgg ctgttaggta
       61 cgaactgccc agcccgccct taaccccgtt aaccgcattg gctgcaataa gcacgttggt
      121 gcttgttcct tgttcaatat ctttcccgaa tgcaacgggt atttcaagca gcatatcgga
      181 gttgttagac tcaacttgca gcattgccct ctgatagctt tctgccacat cggttatccg
      241 aaaatatccc gacgactgaa tcttttgtat cagcttttgc gactggatgc tccggtcgtg
      301 atcaacaaca caaaggttga tgttcttgat ctcaaaattg gcaatgagcg gaaaaatgag
      361 catggccatt aatggcagca ttactatcat cttaggcaga aaactgttcc tcacgaactg
      421 ctttatttct ttttcgagta agtatcgtaa cataacattc ataaattatt aacccaaata
      481 acacaagaga gttacatgtt tttcaattat ctaacatctt caacaagcaa tcattccagc
      541 ctgattttga accgtttgat gctggcaaaa acaataaggc tccccatgcc aaccagaatg
      601 ataatttcgc gtaaaaccga ggttatgcca agtcctttaa tcattacgtt tcgcatggca
      661 attatgaacc atttggccgg gataatgttg gcaaaccact gtaaagccac gggcatactt
      721 tcgagcggaa acatcatacc cgataggtaa accactggca acattaaacc tgcacccgaa
      781 ataagcagcg caacaatttg tttctcggct actgttgata taaacagccc caaagccagc
      841 gatacaaaaa tgtagatgag cgaaataagt accaccaacc caaaacttcc aacaatggga
      901 acacctagca tataatgtga cagcaataag atagtggaca ggttcaccag cgatatgcag
      961 aaatacggta tcattttcga gaaaattatc aggaatggcc tcatgggcga cacaagcaat
     1021 acttccatag tgccgtgttc tttttcgcgg gtaagcgaca ccgaggtcat gagcgcacaa
     1081 atgaggataa taataacccc cataattccc ggcacggtat tgaacgagtt tttcatcgat
     1141 gggttgaaaa gcaacttaac ttggggcgaa atcaacaacg ctttttcatc ttctttggaa
     1201 agttcttttg cgtatagtga aatcaaactc gaagcatacg t
//
LOCUS       DAOPUW010000648         1228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_131591_length_1228_cov_3.007673, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000648 DAOPUW010000000
VERSION     DAOPUW010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131591_length_1228_cov_3.007673"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PKJ43_09490"
     CDS             complement(<1..741)
                     /locus_tag="PKJ43_09490"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ72845.1"
                     /translation="MRAIFSTIFILSALSLLGQNGVIKGKIVDATTNEPLPFVNVVVI
                     GTSTGSVSDLDGNFQIFGLQPGFTRLSASFIGYNTTVSSEIEVSNSKVSNIEIAMQSA
                     DTELSEVTVTASPFRKTEESPVSLRSIGVGEIETNPGANRDISKVIQSFPGVASTPAF
                     RNDIIIRGGGPSESVFYLDGIEVPNINHFSTQGSSGGVVGIINADFIREVKYYSGAFP
                     SNRGEALSGILEFNQIDGNSERPKFKATL"
     gene            complement(826..>1228)
                     /locus_tag="PKJ43_09495"
     CDS             complement(826..>1228)
                     /locus_tag="PKJ43_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72846.1"
                     /translation="YAKAIGGKLLPSPERPVPDAITSATPTGDFILESVIKNKPEGKF
                     RVMMEINQTWDWNEFWNNSLYPNDFEYKASCQPALVYAVIVDPSQKGKEFYLNPIGHS
                     HFSGQNGDLFTDITTITTAKEIAHKVYVKIK"
BASE COUNT          317 a          256 c          263 g          392 t
ORIGIN      
        1 caaagtcgcc ttgaactttg ggcgttcgct gtttccgtca atttggttaa actcgagtat
       61 gccgctgagt gcttcgcccc ggttcgatgg gaaagcacca gaataatatt ttacctcgcg
      121 gatgaagtcg gcgtttataa tacccactac accacctgat gaaccttggg ttgaaaagtg
      181 gttaatattt ggcacttcaa tgccatcgag atagaaaacg ctttccgatg gccctccacc
      241 tctgattatg atgtcattcc gaaaagcagg cgttgaggca actcccggaa acgattgaat
      301 aactttagaa atgtcgcggt ttgcaccagg gttggtttca atttcgccaa cgccaatact
      361 gcgcaatgaa accgggcttt cttctgtttt gcggaacgga cttgcggtaa ccgtaacttc
      421 actcagttcg gtatcggccg attgcatggc aatttcgata ttcgagactt tcgaattact
      481 gacttcaatt tctgaagaaa cagttgtatt gtatcctata aatgaagcag ataaacgcgt
      541 aaacccaggt tggaggccaa aaatctgaaa atttccatca aggtcgctca cagaaccggt
      601 tgaagtccca ataactacaa catttacgaa aggtaagggt tcgttggttg tcgcatcaac
      661 aattttccct tttatcactc cgttctgccc caaaagcgaa agggcagata atataaatat
      721 ggtgctaaat attgctctca ttatttgttg ttttggtttt ttattgttgc catttttcca
      781 ttgctgtaca gttggcatat actgtacttt ctctttttca atgcgttatt taattttaac
      841 atacacttta tgggcaatct cttttgctgt ggtaatggta gtaatgtcgg taaaaaggtc
      901 tccgttttgc ccggaaaagt gtgaatgtcc tattgggttt aggtagaatt ctttaccctt
      961 ttgagaaggg tcgactatta ccgcatagac aagcgcaggc tggcacgatg ctttatattc
     1021 aaagtcgttg ggatataaag aattattcca aaattcattc cagtcccaag tttggtttat
     1081 ttccatcatt accctgaatt tgccttcggg tttgttttta attacactct cgagaatgaa
     1141 atctccagtt ggtgtcgccg atgtaatggc atctggaaca gggcgttcag ggctagggag
     1201 caactttcct cctattgctt ttgcatat
//
LOCUS       DAOPUW010000649         1223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_132462_length_1223_cov_2.841610, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000649 DAOPUW010000000
VERSION     DAOPUW010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132462_length_1223_cov_2.841610"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="PKJ43_09500"
     CDS             <1..844
                     /locus_tag="PKJ43_09500"
                     /inference="COORDINATES: protein motif:HMM:NF024958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HNZ72847.1"
                     /translation="RAFVNADFGLSFINESNKIRKSVPPNTSKELKAEFKEIETEIRD
                     VVKSQSGRLEKYMLDERRWAAEDWHNFFFMNPILFVYAMKLVWGVFDENNILEQVFYC
                     TEDASLYDVNDDEVAVPADRFVGILHPALLSIADRKLWFDKIFDMQLSTIFPIFERTV
                     FSVADDELELNYTKHFFNQDVPKGADFVNTFLVKRNWLKTSGDGARSNFTKHFRDGKI
                     IADANIDGPMAFYSGGNTKATVFEISFITVKEMRREKVLLKDVPPVFYSEVMFDIDQL
                     LKAE"
     gene            849..>1223
                     /locus_tag="PKJ43_09505"
     CDS             849..>1223
                     /locus_tag="PKJ43_09505"
                     /inference="COORDINATES: protein motif:HMM:NF040143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HNZ72848.1"
                     /translation="MAVHYLGIRHHGVGSAKNVGAQLAALKPDMILVEGPTEISDVLS
                     VVGNPGLVPPAAIMAYNSIDPKESIFFPFAEYSPEWVAILYANKNNIPVRAIDLPASI
                     SFTLRSESSNEEQDNNEPLRDPL"
BASE COUNT          355 a          225 c          280 g          363 t
ORIGIN      
        1 ccgggccttt gtaaatgctg attttgggtt gagttttatc aacgaatcca acaaaatcag
       61 gaaatcggtg cctccaaata cttcaaaaga acttaaggca gagttcaaag aaattgaaac
      121 ggagatacgc gatgtggtta aatcgcaaag cgggcgtctg gagaaataca tgctcgatga
      181 acgtcgttgg gcagccgaag actggcataa ctttttcttc atgaacccta ttttgtttgt
      241 ttatgccatg aagctggttt ggggtgtttt tgatgaaaat aatattttag agcaagtatt
      301 ttattgtacc gaagatgcat ctctctacga tgttaatgat gatgaggttg cagttccggc
      361 tgatcgcttt gtcggtattt tacatccggc cctgttaagt attgccgatc ggaagctttg
      421 gttcgataaa atcttcgaca tgcagttatc gaccattttc cccatttttg aaagaactgt
      481 tttttctgtt gccgacgatg agctggagtt aaactataca aaacacttct ttaatcagga
      541 tgtacctaag ggagccgact ttgtgaacac ttttcttgtg aagaggaact ggctgaaaac
      601 ctcgggcgat ggagcccgct caaattttac aaagcatttc agggatggaa aaattattgc
      661 cgatgcaaat attgatggac caatggcttt ttattccggg ggaaatacaa aagctacggt
      721 ttttgaaatt tctttcatca cggtaaagga aatgcggcgc gaaaaagttt tgctaaaaga
      781 tgtcccgcca gttttctatt cagaagtaat gtttgacatc gatcaattac taaaagcaga
      841 atagcaaaat ggcagttcat tatcttggga taaggcatca tggggttggt tcagcaaaga
      901 atgttggggc acaacttgcg gcattgaaac ccgatatgat tttagttgaa ggccctaccg
      961 aaataagcga tgttttgtcg gtcgttggca acccggggct tgtaccacct gctgcaataa
     1021 tggcttataa ttctattgat ccgaaagaat cgatattttt tccttttgcc gagtattcgc
     1081 cagagtgggt ggctattctg tatgccaata aaaacaacat ccctgttcgg gcaatcgatt
     1141 tgccggcatc aataagtttt acattgagaa gcgaatcttc aaatgaagaa caagataaca
     1201 acgagccatt aagagaccca ttg
//
LOCUS       DAOPUW010000650         1221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_132779_length_1221_cov_3.331046, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000650 DAOPUW010000000
VERSION     DAOPUW010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132779_length_1221_cov_3.331046"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PKJ43_09510"
     CDS             <1..321
                     /locus_tag="PKJ43_09510"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNZ72849.1"
                     /translation="ASGKLNNKAVVVMAEIGIDISHHTSDSVQKYLGEEWDYVITVCG
                     GANESCPAFLGKVKHRLHIGFDDPSEAWGEDVFVLKEFRRVRDQIRDGFYKLYEEQIK
                     PQLQ"
     gene            318..773
                     /locus_tag="PKJ43_09515"
     CDS             318..773
                     /locus_tag="PKJ43_09515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HNZ72850.1"
                     /translation="MNRTERAKEFFANHYNCSQSVLTAFAPELGLSVDQSLKVACAFG
                     AGIGRQQLTCGAVTGALMALGLKYGKALNDSEDKKLNTYAKTQAFCEAFKKEHGSINC
                     RELLNGLDMNNPKDKERILADDLFNTKCPVYVASAVQIAEQIMKNQEEE"
     gene            1034..>1221
                     /locus_tag="PKJ43_09520"
     CDS             1034..>1221
                     /locus_tag="PKJ43_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72851.1"
                     /translation="MRALSLFLVCTTLLITYLPSEASDKSTKTLKDAYNDAFKMGCSV
                     NASIVSGADAKSSEIVKA"
BASE COUNT          388 a          231 c          267 g          335 t
ORIGIN      
        1 gcttcaggaa aattgaataa caaagcagta gtggtcatgg ctgaaattgg tatcgacatt
       61 agtcatcata cctccgattc agttcagaaa taccttggcg aagagtggga ttatgtgatt
      121 accgtttgtg gtggcgccaa tgaaagttgt ccggcatttt tgggtaaggt aaaacatcgc
      181 ctgcatattg gtttcgacga tccatcggaa gcctggggtg aagatgtgtt tgtattgaaa
      241 gaattccgca gggttcgcga ccaaatacgc gatggctttt ataaattata cgaagaacaa
      301 ataaaacctc aattacaatg aacagaacag aacgggcaaa agaatttttt gctaaccatt
      361 acaactgctc gcagtcggtg ttaactgctt ttgcacccga attgggactc agtgtcgacc
      421 aaagtttgaa agtagcttgt gcctttggcg ccggaatcgg acgccagcaa cttacctgtg
      481 gcgcggttac cggagccctg atggccctgg gcttgaaata cggaaaagcc ttaaacgatt
      541 ctgaagataa gaagttgaat acctatgcca aaacacaggc attttgcgag gcatttaaga
      601 aagaacacgg ctcaatcaac tgccgcgaat tgctaaatgg cctggacatg aacaatccaa
      661 aagacaaaga acgtattctg gctgatgatc ttttcaatac gaaatgccct gtttatgttg
      721 caagcgctgt acaaattgca gaacaaatca tgaaaaatca agaagaagag tgagcataag
      781 caaattgttt tttcatttgg atttaattgg aaaattgcgg gatctgtccg gatatcaaat
      841 aggtgaataa cattagccca gattgatgtt atagcttttt cctctatgcc gtactcaacg
      901 tgaaacagac atcagatttt cgtacctaat taaatgataa atcgaatcat ttcgatattc
      961 gtgccttctt tataactcaa atgcattatt tttgaggttg aaagtacgta aaaccaacta
     1021 atcaatattc aatatgagag cattaagcct attcctggtt tgcaccactc tgcttataac
     1081 ttatttgcca tcagaggctt cagataaaag cacaaaaaca ttgaaagatg cgtataacga
     1141 tgcctttaag atggggtgtt cggttaacgc gtcaattgtt agtggggctg atgcaaaatc
     1201 gtcagaaatc gtaaaagccc a
//
LOCUS       DAOPUW010000651         1221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_132893_length_1221_cov_1.916810, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000651 DAOPUW010000000
VERSION     DAOPUW010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132893_length_1221_cov_1.916810"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PKJ43_09525"
     CDS             <1..665
                     /locus_tag="PKJ43_09525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ72852.1"
                     /translation="TLLKQLADDYRNNKEFNENASHELQTHLAIIRANTEKLLATNQE
                     NEDNIPPITKIHSAASQLSQIQKSLLLLSKISNREYSNISTVDLQSLVKSSLETFAEA
                     ISLRNISLETTLGTCFIDADAGLAGILINNLLKNAVKYNISNGFIRIELNAQCLSVAN
                     SGDLFEGDPSAMFERFSKGEKGNTGIGLAIVKQICELYGFRIEYLIKDKNIHKISIFF
                     KN"
     gene            735..1181
                     /locus_tag="PKJ43_09530"
     CDS             735..1181
                     /locus_tag="PKJ43_09530"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HNZ72853.1"
                     /translation="MNTQHTIKTIAIVILSVIIFASCEKETIIDSNSLPKTAQEFITT
                     HFAGQSIVSVVSDKELLESSFEVSLSEGYNLNFNRKGECTKIDGNEKRVPDSAVSQKI
                     TEYVEKNYSPSFITNWEKETVGQSVELSNDIELRFNDKGEFTRIDN"
BASE COUNT          448 a          258 c          221 g          294 t
ORIGIN      
        1 ggacactgct caaacaacta gccgacgatt acagaaacaa taaagaattt aacgaaaacg
       61 cttcacacga actccagacc cacttagcca ttatacgtgc caacacagag aaactgctgg
      121 ccaccaatca ggagaatgaa gataatattc cacccattac aaaaatacat tctgcagcaa
      181 gtcagctgtc gcaaattcag aagtcgctgt tacttctgag caaaatttca aacagggaat
      241 actccaatat cagcacggtt gacttacaat cccttgtaaa aagctcgctc gaaacttttg
      301 ccgaggccat ttcgctcagg aacatttcac tcgaaacaac actcggcaca tgtttcattg
      361 atgccgatgc cggattggcc ggaatactaa taaataactt gctgaaaaac gccgtgaagt
      421 acaacataag caacggattc atcaggattg aactaaacgc ccaatgcctt tcagtagcaa
      481 acagtggcga cctgttcgaa ggagacccat ctgccatgtt tgaacggttt tcgaaaggag
      541 agaaaggcaa cacgggcata ggcctggcca ttgtgaaaca aatctgcgaa ctttacggat
      601 tccgtattga atatttaata aaagacaaaa acatccacaa aatatcaatc tttttcaaaa
      661 attagttttt tctccaaaat gtctccaaaa tcgtgtcgta gctttgctgt ataaacttaa
      721 aaaatacagt agacatgaat acacaacaca caattaaaac aattgcaatt gtcatattat
      781 cagtcataat atttgccagt tgcgaaaagg aaacaataat cgacagcaat agtttgccca
      841 agactgccca agagtttatt acaacacatt tcgccgggca aagcattgtt tcggtagtaa
      901 gtgataaaga acttctggaa tcgagttttg aagtttcact ttccgaaggg tataacctga
      961 atttcaaccg gaaaggtgaa tgcacaaaaa tagatggaaa tgagaaacgc gtgcccgatt
     1021 cggctgtatc acaaaaaatt actgaatatg tagaaaagaa ttattcgcct tcgttcataa
     1081 ccaattggga aaaagaaacc gttggccaaa gtgtagagtt atcgaacgat attgaactgc
     1141 gcttcaacga caaaggagaa ttcacccgca ttgacaacta ataaacaagt attaacaatc
     1201 aaataaaata acaactatga a
//
LOCUS       DAOPUW010000652         1212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_134383_length_1212_cov_4.637857, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000652 DAOPUW010000000
VERSION     DAOPUW010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134383_length_1212_cov_4.637857"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..879)
                     /locus_tag="PKJ43_09535"
     CDS             complement(76..879)
                     /locus_tag="PKJ43_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72854.1"
                     /translation="MKIIKSQAVSPFGGINFVIKEAIDLKINTLLNNNLPSLPTQSQY
                     NWFDIFMSYWSVFFCGGDCAEDLSINIKKGLNANPFIKVPSPDRILERIKSLSDGTQK
                     FTTKRGEKDHQFSLAEEMNRLNLKMLSLLPGFQKKDVVLDYDNTVIFTEKADARMTYK
                     KGFGYFPGVGMVGRHTVFVENRNGNSTAHVMQHETIDRMATLLGETGITIGAIRADSA
                     SYGYEIIKSMEKAAKRIFVKARMTNALEEAISGIKEWKEIKIGGVAISE"
     gene            complement(980..>1212)
                     /locus_tag="PKJ43_09540"
     CDS             complement(980..>1212)
                     /locus_tag="PKJ43_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72855.1"
                     /translation="LSLNLGIKNPDEQQEYILSNIAVNEQWTYTLGGIYKHFHKNGFL
                     SVALSRNMLNNDANKYTDNDDSKPENLTLDYT"
BASE COUNT          311 a          266 c          246 g          389 t
ORIGIN      
        1 agaatgaata actaaaaaat gtattattgt agcatctttc aaagtggcac tgtttgaccg
       61 aaacgcctgg catggtcact ccgaaatagc caccccccca attttgatct ctttccattc
      121 ttttatgccc gatattgctt cttcgagcgc attggtcatc cttgccttaa cgaaaatccg
      181 cttggccgct ttctccatcg acttgataat ctcataacca taagaggcag aatctgccct
      241 tatcgcaccg attgttatgc cggtttcgcc cagaagagtg gccatcctgt caatggtttc
      301 gtgctgcatg acatgggccg tgctgttgcc gttccggttt tctacgaaga cagtgtgcct
      361 cccaaccatg ccaaccccag gaaaataccc aaaccccttt ttataggtca tccgggcatc
      421 ggctttttcg gtaaagatga ccgtattgtc ataatccaac acaacatctt ttttctgaaa
      481 gcccggcaac aatgacaaca tctttaagtt tagtcggttc atctcttctg ccaacgaaaa
      541 ttggtggtct ttttcgcccc gtttggtggt gaatttttgt gtgccgtccg acaaagattt
      601 gatccgttcc agtatcctgt cggggcttgg tactttaatg aaaggattgg cgttcagccc
      661 ttttttgata ttgatggaca agtcttcggc acaatcgcca ccgcaaaaaa acacagacca
      721 gtacgacatg aatatgtcga accaattgta ttgtgactgt gttgggaggc ttggtaaatt
      781 attattcaac aatgtgttga tttttaaatc aatggcttct ttgattacaa aattaatccc
      841 gccaaaaggt gaaacggctt gactttttat tattttcata tcggtatttg ttgcaacacc
      901 aaattaggtg aaaatgcctt agctgtaaag ctttatcgaa taaagtttta cagctttttt
      961 caacatactc ttgcggaatc taggtgtaat cgagcgtcag attctcaggt ttggaatcgt
     1021 cattgtcggt atatttgttt gcatcgttgt taagcatatt ccggcttaag gcaactgata
     1081 gaaacccatt cttgtgaaag tgtttataaa ttccgcctaa cgtgtaggtc cattgttcgt
     1141 taactgcaat gttcgaaaga atgtattcct gctgttcatc gggattcttg ataccaaggt
     1201 tgagtgaaag ct
//
LOCUS       DAOPUW010000653         1212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_134422_length_1212_cov_2.770959, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000653 DAOPUW010000000
VERSION     DAOPUW010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134422_length_1212_cov_2.770959"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PKJ43_09545"
     CDS             complement(<1..325)
                     /locus_tag="PKJ43_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ72856.1"
                     /translation="MYVPELFPDWSDKTILIVEDEEIIRIFFDGALKVTKAKLLFAEN
                     GYDGIKIALSNDSIDCVLMDIRMPILDGYEAIKSIKATKPGLPVIVQTAYALSHDRKK
                     AYEAGC"
     gene            598..1032
                     /gene="dut"
                     /locus_tag="PKJ43_09550"
     CDS             598..1032
                     /gene="dut"
                     /locus_tag="PKJ43_09550"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNZ72857.1"
                     /translation="MKVKIVNKSSNALPGYSTSLSAGMDLRANLTQDIVLKPLQRTLV
                     PTGLFIELPEGYEAQIRPRSGLALKHGITVLNTPGTIDADYRGEIGVILVNLSDEEFV
                     IKHGERICQMIISKHETVGWEEVEVLEETARGAGGFGHTGKK"
BASE COUNT          406 a          223 c          256 g          327 t
ORIGIN      
        1 cgcaaccggc ttcgtatgct tttttgcggt cgtgcgataa ggcataggct gtttgtacaa
       61 ttaccggtaa tcctggcttt gttgctttaa tcgatttaat agcctcataa ccatcaagaa
      121 ttggcatcct gatatccatc aatacacaat caattgaatc gttcgataag gcaattttta
      181 taccgtcata cccgttctct gcaaaaagaa gttttgcttt agtgactttt aatgccccat
      241 caaaaaaaat acggataatt tcttcatctt ctacaatgag gatggtctta tccgaccaat
      301 cgggaaataa ctctggaaca tacatagtaa aaacggtata ggtttataaa atgggtcgtt
      361 aaacacatct gctaaattac gtttaaaatt gaaatggggt acaaaatcta caagaatgta
      421 tatcttagca gcttaatttt tcaaaaaagt gcattttgcc ggttatcagt gaataatcaa
      481 tcgtttaaaa cgatataggc ataagagtgt gtgaaaccgt tgccaatgaa gataaaaccg
      541 gaagctccgg ggtattatcc tgtgcaacaa tatcgtaaag aaataaacag gtaaaaaatg
      601 aaagttaaga tagtcaataa atcatcgaat gcattgccag ggtacagtac atcgttatcg
      661 gcaggaatgg atttacgagc caaccttacc caggatatcg tattgaaacc gctacaacgc
      721 acgctggttc ctacgggcct ttttatcgaa ttacccgaag gatacgaagc tcagatcagg
      781 ccaagaagcg ggttggcact caagcacggg atcaccgtat taaatactcc cggaaccatt
      841 gatgccgact atcgtggcga aataggggtt attcttgtaa acctttcgga cgaagaattt
      901 gtcattaagc atggtgaacg tatttgtcaa atgatcattt cgaaacatga aactgtgggt
      961 tgggaagaag tagaagtatt ggaagaaaca gcgcgtggcg ccgggggctt tggtcatacc
     1021 ggtaaaaaat gagaacaaaa aatgaggaaa aatattgtat tacttatcgt tggcttacaa
     1081 ttattcggat ggggctgcag caacaggaat atctctcaaa aaccggaggc taaagctaaa
     1141 cctgcacaag ctcaggaaac atctcttgaa gaaagcaaaa aaattgaatt cgaattctat
     1201 ttcatagaag gg
//
LOCUS       DAOPUW010000654         1212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_134483_length_1212_cov_2.080380, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000654 DAOPUW010000000
VERSION     DAOPUW010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134483_length_1212_cov_2.080380"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..>1212
                     /gene="sucC"
                     /locus_tag="PKJ43_09555"
     CDS             142..>1212
                     /gene="sucC"
                     /locus_tag="PKJ43_09555"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNZ72858.1"
                     /translation="MKIHEYQARELLASYGLPVDKAYICRNVDDAVRAYHELDAPLVV
                     VKAQVHTGGRGKAGGVKLAKDEIELRQHAANILGMDIKGFIVDRVLVVKAVNIASEYY
                     VSYVIDRKSKSTILMLSREGGMDIEEVAHNTPEKIHKIVIDPLIGVPDYLAREAAFKL
                     FDDIKQVREATSIFQKLYKLFVDTDASLAEINPLVLTEEGQIKAIDAKMTFDDNALYR
                     HPKIAALFEPTEEEKKEQNAKSKGFSYVHLGGAIGCMVNGAGLAMATMDMIKLYGGEP
                     ANFLDIGGSSNPTKVIEAMKLLLSDKNVKVVLINIFGGITRCDDVARGLLEAFNQIKT
                     DIPIVIRLTGTNEKEGRELLQGT"
BASE COUNT          399 a          233 c          274 g          306 t
ORIGIN      
        1 gtaacttgta ttaactgcga aagaactcca cctctttctt acatttaaag ttaaaaagct
       61 tttttattat tcttttatca ggaatatttt ttacttttac aaaaaaaaag aaaaatcaac
      121 actaaaaaat ctgaaacgct catgaaaatt catgaatatc aggcaagaga attgttagca
      181 tcatacgggc taccggtaga caaagcatat atttgtcgta atgtggatga tgcagtgcgg
      241 gcataccatg agttggatgc tccgttagtg gttgtgaaag cgcaggttca tacaggagga
      301 cgcgggaaag cgggaggtgt gaagctagcc aaagacgaaa tagaactccg gcaacacgct
      361 gccaatattt taggaatgga tataaaggga tttattgtgg acagagttct ggtagtcaaa
      421 gccgtaaaca ttgcttcgga atactatgtg agttacgtga tagaccgtaa gtcgaaatct
      481 accatcctga tgttgagccg tgaaggtgga atggatattg aagaagtagc gcataacaca
      541 cccgaaaaaa tacataaaat agttatcgac cctctcatcg gcgtacccga ttacctggct
      601 cgcgaagcgg cttttaaact tttcgacgac attaaacagg ttcgcgaagc aacatcaatt
      661 ttccagaaac tatacaagct ttttgttgat accgatgcat cactggcaga aatcaacccg
      721 ctggtgctta ccgaagaagg ccagattaaa gctattgatg ccaaaatgac tttcgacgat
      781 aacgctcttt atcgtcatcc caaaattgcc gcccttttcg aacctaccga ggaagaaaag
      841 aaagaacaaa atgctaaaag caaaggattt agctatgtac atctgggcgg agcaatcgga
      901 tgcatggtga atggcgccgg actggcaatg gcaacgatgg acatgataaa attgtacggg
      961 ggagaacctg ccaactttct cgatatcggc ggaagctcta accccacgaa ggttatcgaa
     1021 gccatgaaac ttttacttag tgacaagaat gttaaagtcg tgttaatcaa tattttcgga
     1081 ggaattaccc gttgcgacga tgtggcaaga ggattactgg aggctttcaa tcagattaaa
     1141 accgatatac ccattgtaat acggttgacc ggaaccaatg agaaagaagg tcgcgaatta
     1201 ttacaaggca ct
//
LOCUS       DAOPUW010000655         1209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_134948_length_1209_cov_3.688908, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000655 DAOPUW010000000
VERSION     DAOPUW010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134948_length_1209_cov_3.688908"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..1051)
                     /gene="folP"
                     /locus_tag="PKJ43_09560"
     CDS             complement(158..1051)
                     /gene="folP"
                     /locus_tag="PKJ43_09560"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNZ72859.1"
                     /translation="MLRFAMLTPATDKPYFRKKSTIRYNGELVNLSCPMVMGILNVTP
                     DSFYDGGKHMTIEKAMKRAGEMVSEGASIIDIGASSTRPGAAEVSPEEELARLLPVAK
                     EIRKNFQDIIISIDTYNSGVARSVIEEIGDCIINDISGGKLDPKMFETVAKLKVPYIL
                     THIQGTPKTMQQNPNYENVTKEVLKELSEGIFKLHELGVSDVIIDPGFGFGKTLEHNY
                     ELFGNLDAFRMFELPILVGISRKTMIYNLLNTTPDESLNGTTVLNTLALMAGATFLRV
                     HDVKPAVEAIKIVEQLKKCSL"
BASE COUNT          357 a          237 c          240 g          375 t
ORIGIN      
        1 gcgcgaataa ctatccaggt taagtagaag atgaacatgc ccataactat actaaaagca
       61 gcagtccctt taatcagggc ataaagttca tagattagaa atgcaacaag gagaatatct
      121 agaatgtcga caaatcgcag agtgataaat gcttcgatca taaggaacac tttttaagct
      181 gttcgacgat ttttatggct tcgacagcag gtttaacatc atgaactcgc aaaaaggttg
      241 ccccagccat tagtgccagg gtattaagta cagttgtgcc gttcaaactt tcatcaggag
      301 ttgtattcag tagattgtat atcattgttt tacgtgaaat accaaccaaa ataggcaact
      361 cgaacatgcg aaatgcatcc aggtttccaa ataactcgta attatgttca agtgtttttc
      421 caaatccaaa ccccgggtca ataattacat ctgatactcc aagttcgtga agcttgaaaa
      481 tgccttcact aagttctttc aaaacttctt tggtaacatt ttcgtagtta ggattttgtt
      541 gcattgtttt aggagttcct tgaatatgtg tcagaatata aggaactttc aattttgcaa
      601 cggtttcaaa catcttaggg tcaagttttc cacccgaaat atcgttgatt atgcaatcgc
      661 caatttcttc tataacagag cgggcaacac cactgttata ggtgtcgatt gagatgatta
      721 tgtcttgaaa atttttccgt atttctttag ccacgggcag aagccgggca agttcttctt
      781 ccggcgatac ttcagcagct ccggggcggg ttgacgaagc gccaatgtcg attatcgatg
      841 ctccttcaga aaccatttca cctgcacgtt tcatggcttt ttcaatagtc atgtgtttgc
      901 ccccgtcgta aaacgaatcg ggcgtaacgt tcagtattcc catcaccatt gggcatgaca
      961 ggtttaccag ttcgccgttg taacgaatcg tgcttttttt cctgaaatat ggtttgtctg
     1021 tagctggtgt tagcatggca aatctcaaca attagggcta tttcgtttca caaatctaca
     1081 ataattcggc ttgatgttct gaaatttttt acttttattg gccaaaaata atgatgatgg
     1141 aaaaaacaaa acaacagttc gacagtgtca tagaaatatg taaatcggta tttgtcaaga
     1201 agatgatcg
//
LOCUS       DAOPUW010000656         1208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_135186_length_1208_cov_2.907199, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000656 DAOPUW010000000
VERSION     DAOPUW010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135186_length_1208_cov_2.907199"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PKJ43_09565"
     CDS             <1..214
                     /locus_tag="PKJ43_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72860.1"
                     /translation="LAKSISISIPEMDENVGLFDYSDQYDKYVMVKNWFEKNPERLAV
                     DISAYIKEMAFVPSFSEKYYFDNDID"
     gene            322..678
                     /locus_tag="PKJ43_09570"
     CDS             322..678
                     /locus_tag="PKJ43_09570"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNZ72861.1"
                     /translation="MKKIAFSILIASMALSSFAGNDKTQNELEISTKTNANQEISGIV
                     TDKQSNEALVGVEVIIEGTNQKVYTDFDGKFTFSNLKPGEYNLVASYISYEKNIVENI
                     DVKKDNLVTICLEASN"
     gene            complement(872..>1208)
                     /locus_tag="PKJ43_09575"
     CDS             complement(872..>1208)
                     /locus_tag="PKJ43_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNZ72862.1"
                     /translation="KQHVYLNGKRIGEEMFQKTGRYTLEPEMAMLKVGQNELTFVATP
                     YYLEQTWSEPNTDPGIIQLIMPAPAWKRSLFSGKAQVIVQSGKGGGEIKLTAKSEGLI
                     PTQLSLISE"
BASE COUNT          398 a          250 c          211 g          349 t
ORIGIN      
        1 gctggcaaag tccatctcaa tctcgatccc cgagatggac gaaaacgtag ggctattcga
       61 ttattccgac caatacgaca agtatgtaat ggttaaaaat tggtttgaaa agaatcccga
      121 aaggctggct gtcgacatct cggcctatat aaaagaaatg gcttttgtcc cttcattttc
      181 cgaaaagtac tatttcgaca atgacattga ttaatccgat ttgccccatc tttttaatcg
      241 aactgtaata acagtgcaac aggcatgtaa cacctctggc gtagttttgc acagtaatcg
      301 agaacttaaa aaagaaacaa aatgaaaaaa atagcattta gcatccttat tgcatcgatg
      361 gctttatcct catttgccgg caacgacaag acacaaaacg aattagaaat cagcacaaaa
      421 accaatgcca atcaagaaat atcaggtata gttaccgaca aacaatcgaa cgaggcactg
      481 gtgggcgttg aagtaataat tgaaggtacc aaccaaaaag tgtataccga ctttgatgga
      541 aaattcacat tcagcaatct taaaccgggc gaatataatc ttgtagcctc atacatttcg
      601 tatgaaaaaa acatagtcga gaacatcgac gttaaaaagg ataatttggt aacaatctgc
      661 ctcgaagcat ctaattgatt tgttacaaat atttaattga ttattaactc attaacaact
      721 atttataagt aattttaccc caagttaaat agttcttaaa atctcactca gaatttttac
      781 aggaaggtaa acctgttttc tatttaagat tgatcagccg ctccctgggg agcggctttt
      841 caaattcaac ccctataata attactaaaa tctattccga aataagcgat aattgagtag
      901 gtataagccc ttctgatttt gctgtcagtt taatttctcc tccacctttt cccgactgaa
      961 caataacctg agctttacca ctaaagagtg agcgtttcca ggccggtgca ggcataatta
     1021 actgaataat cccaggatcg gtattgggtt ccgaccatgt ttgctcgaga taatatgggg
     1081 ttgccacaaa agttaattca ttttgtccaa ctttaagcat cgccatctca ggctcaaggg
     1141 tataacgccc tgttttctga aacatctctt cgccaattcg cttaccattc aggtatacat
     1201 gttgcttc
//
LOCUS       DAOPUW010000657         1203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_136026_length_1203_cov_3.500871, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000657 DAOPUW010000000
VERSION     DAOPUW010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136026_length_1203_cov_3.500871"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..658)
                     /locus_tag="PKJ43_09580"
     CDS             complement(23..658)
                     /locus_tag="PKJ43_09580"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNZ72863.1"
                     /translation="MIDQRIVKNVILDMGGVLVDIAPQNTIKALKHILQPCVAESELM
                     NSHQEVLIAMETGKWTKQEFYDFFKGKCKNGVIEEEIIDAWCAMVLDLPRKRVEMVKK
                     LASKYSVFLLSNTNTFHIKCVESDFKNRYNYSYKKLFTKIYYSSEIGLRKPDPACFKF
                     VLEDASLNPAETVMVDDVEENCLAAENAGMQALRVPENTGLEAVIHHLLPN"
     gene            705..>1203
                     /locus_tag="PKJ43_09585"
     CDS             705..>1203
                     /locus_tag="PKJ43_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72864.1"
                     /translation="MRINGQKFHTIWFDINHPETVKIIDQQKLPFEFCIKSFHSFYDA
                     FVAIKDMTVRGAPLIGVTAAFGMYLGLYGCTSELPADEYANALAREMKTARPTAVNLS
                     YAVDLVMNALQGCDTFDEMAGKALETALQLKQSEIESSSAIGDFGLTLIEQIAAQKPG
                     KPVNIL"
BASE COUNT          341 a          250 c          259 g          353 t
ORIGIN      
        1 tttcccggaa aatcaggatt gactagtttg gtaatagatg atgaattacc gcttcaaggc
       61 cagtgttctc aggaaccctt aaggcttgca ttcctgcatt ttcggcagca aggcaatttt
      121 cttccacatc gtcaaccata acagtttcgg cagggttgag cgaggcatct tcaagtacaa
      181 acttgaaaca agccggatca ggcttgcgta gcccgatttc tgaagagtaa taaatctttg
      241 taaaaagctt tttgtacgag tagttatatc tgtttttaaa atcagattca acacatttaa
      301 tatgaaaagt attggtattg cttagcagga agaccgaata ttttgacgct agttttttga
      361 ccatttcgac ccttttacga ggtagatcga gaaccattgc acaccatgca tcaatgattt
      421 cttcttcaat taccccgttt ttgcatttgc ctttaaaaaa atcgtaaaac tcttgtttgg
      481 tccacttgcc ggtttccata gcaataagca cttcctgatg gctgttcatc aactcgcttt
      541 cggcaacaca aggttgaagg atgtgtttca gcgctttgat ggtattttgg ggtgctatgt
      601 ctacgagaac gcctcccatg tcgagaatga cattttttac aattcgttgg tcgatcatga
      661 tcaattatta taaattgcca caaagttagc atcgatttgt aatcatgaga ataaatgggc
      721 aaaaatttca cacaatttgg ttcgatatta accatcccga aacggtaaaa attatcgatc
      781 agcaaaaact tccttttgag ttttgcataa aatcctttca ttcattttac gatgcttttg
      841 tcgccataaa agacatgacg gtgcgtggag ccccgttaat tggagtgact gcggctttcg
      901 ggatgtacct tgggttatac gggtgtacca gcgaacttcc ggcagatgaa tatgccaatg
      961 cacttgcacg cgaaatgaaa acggcacggc ctaccgcggt aaacctctcc tatgccgtcg
     1021 atctggtaat gaacgctttg caggggtgcg acacgtttga tgaaatggcc ggtaaagctc
     1081 ttgaaacagc tctgcaactg aaacaatcag aaatagaaag cagcagtgcc attggcgatt
     1141 ttgggctaac gcttattgag cagatagccg cgcaaaagcc ggggaagccg gtaaacatac
     1201 tta
//
LOCUS       DAOPUW010000658         1203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_136101_length_1203_cov_2.216028, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000658 DAOPUW010000000
VERSION     DAOPUW010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136101_length_1203_cov_2.216028"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PKJ43_09590"
     CDS             complement(<1..748)
                     /locus_tag="PKJ43_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNZ72865.1"
                     /translation="MTISAHKMGDWLPTSAKEIKALGWESVDVILFSGDAYVDHPSFG
                     AAVIGRVLEAEGLRVAIIPQPNWRDDLRDFKKLGVPNLFFAITGGCMDSMVNHYTANK
                     RLRSDDAYTPDARPGMRPDYATTVYSNVLKDLFPNVPVIIGGIEASLRRVTHYDYWSD
                     TLKPSILFDSKADLLVYGMGEQAIIEIARLMKSGMPVEKIRNVLQTAWLQNEPPIELQ
                     KQLGTIITLNSHEVCLADKIKYATNFRHVEE"
BASE COUNT          344 a          272 c          227 g          360 t
ORIGIN      
        1 gctcttcaac atgcctgaaa tttgttgcat attttatttt gtcggcaagg catacttcat
       61 gactgtttaa tgtaataata gttcctaact gtttctgaag ttcgattggt ggctcattct
      121 gtaaccaggc tgtttgtaaa acgtttctta ttttttcgac aggcatgcct gatttcatta
      181 gccttgcaat ttcaattata gcctgttcac ccataccata aaccagcaaa tcggcttttg
      241 aatcgaacaa aatcgagggt ttcaaggtat cgctccaata atcgtaatgg gtgaccctcc
      301 tcaacgaagc ttcaataccg ccaataatca caggaacatt ggggaaaagg tcttttagca
      361 cgttgctata caccgttgta gcataatcgg gacgcatacc aggccgggca tcgggggtgt
      421 aggcatcgtc agaacgcaac cttttgtttg ccgtgtaatg gtttaccata ctgtccatac
      481 acccgcctgt aatggcaaaa aataaattgg gaacgccaag tttcttgaaa tcgcgcaaat
      541 cgtcgcgcca attgggctga ggaataattg caacacgaag cccttcagcc tcgagtacac
      601 ggccaataac cgcagctcca aacgaaggat gatcgacata agcatctccg ctaaagagaa
      661 ttacatcgac actctcccac ccgagggctt ttatttcttt tgccgacgtg ggaagccaat
      721 ctcccatttt atgtgctgat atggtcacaa ctattttctt gaaattcgga caaagataac
      781 cgtttcttat ggcaatggtt cactaataga atatcatgat gccataatac aacctatttt
      841 tcacacgcaa aaagagcctc tgaaaataaa ggctctgaac aaaaatccga cgatgactta
      901 caacgaattg tgagttccat cacaatacgg cgggtttttc gtgtgtttac acccgcataa
      961 atatgccttt cctgtattat tcggtttaaa taacagaggt ttaaattcag ttcctttatg
     1021 cgatccatca caataaggct gatttttact ttgcccgcaa gcacaccaat aatacgattt
     1081 atcaacttca aggtcgactg catacggccc tttttttgca atttttggtt ccatatcttt
     1141 cttttttagt tgcgtttccc tgagttcgat ttccagtaaa aatcaaaaaa agtcgaatac
     1201 agg
//
LOCUS       DAOPUW010000659         1199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_136729_length_1199_cov_3.941434, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000659 DAOPUW010000000
VERSION     DAOPUW010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136729_length_1199_cov_3.941434"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PKJ43_09595"
     CDS             complement(<1..620)
                     /locus_tag="PKJ43_09595"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HNZ72866.1"
                     /translation="MSKVNNVAQLIGKTPLIKLQKLTQNIEATIYVKLESMNPGGSVK
                     DRLAFAMIDAAEKKQLINSKSVIIEPTSGNTGIGLAMICAVKGYRLIIVMPESVSVER
                     RLILKAYGAELYLTPSKDGMKGAIAKANELSKSIGNSFIPMQFENEANAEFHRKTTGP
                     EIWEDTNGEVDIFVAGIGTGGTFSGVSEILKSKKNTIKAIAVEPEDS"
     gene            complement(835..>1199)
                     /locus_tag="PKJ43_09600"
     CDS             complement(835..>1199)
                     /locus_tag="PKJ43_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ72867.1"
                     /translation="ARLQIKEEQGIAPNESMVFFDTWLIITKVWLDFVFGKSPDWLKH
                     HISASKIDLFLVCDIDIPWEPDPVRENGGETRKLLQQIYINELRNFGFAYEIVSGKGI
                     ERTQNAINIVDRFLSASR"
BASE COUNT          338 a          230 c          232 g          399 t
ORIGIN      
        1 gaatcttcag gttcgacagc tatggctttt atggtatttt ttttcgattt caggatttca
       61 ctaacgcctg agaaagttcc gccagtacct atgccggcaa caaaaatatc gacttcacca
      121 ttggtatctt cccatatttc tgggccagta gttttacgat gaaattcagc attggcttca
      181 ttttcgaact gcatgggaat aaatgagttg cctatagatt tgcttagttc gttagctttt
      241 gcgatagccc ctttcattcc atcttttgag ggagtgaggt atagttcagc gccataggct
      301 tttaaaataa gacgacgttc aactgaaacg ctctcgggca ttacaattat tagccggtaa
      361 ccttttacgg cacagatcat agccaggcca attcctgtat tgccgcttgt tggctcaata
      421 attaccgatt tgctgttgat cagttgtttt ttttctgcag catcgatcat agcgaaagcc
      481 agccggtctt tcaccgagcc tcccgggttc atcgattcta atttaacata tattgtagct
      541 tcaatatttt gtgtaagctt ttgtaatttg attaatggag tcttgccaat aagctgggca
      601 acattgttca ctttgctcat aattgtagct ttaagttgtt aaaatatgaa ttttataaac
      661 aatcaatgag gaatttagta ggttttttca gttgtgctta caacaaaacg caggaatact
      721 gatatgtgga acaagaatta tcatttctct gtattgatga tgcaatattc ctaaaaaaaa
      781 acgaaaaaac aaattcagca taaaaaggtt gattttacag attgaaagtt aattttaacg
      841 tgatgctgaa agaaatcggt caacaatatt gatggcattc tgggttcgtt caatgccttt
      901 cccgcttaca atttcataag cgaaaccaaa attgcggagt tcattaatgt atatttgttg
      961 caagagcttc cgggtttcgc ccccgttttc ccttacggga tcgggctccc atgggatgtc
     1021 gatgtcgcag accaaaaaaa gatctatttt acttgctgaa atatgatgct ttaaccagtc
     1081 tggcgatttc ccgaagacaa aatcaagcca tacttttgta atgattagcc atgtatcgaa
     1141 gaaaaccatt gattcatttg gggcaatacc ttgttcttct tttatctgga gcctagcaa
//
LOCUS       DAOPUW010000660         1195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_137443_length_1195_cov_3.943860, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000660 DAOPUW010000000
VERSION     DAOPUW010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137443_length_1195_cov_3.943860"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1195)
                     /locus_tag="PKJ43_09605"
     CDS             complement(<1..>1195)
                     /locus_tag="PKJ43_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72868.1"
                     /translation="NKTLINASVLGLIFEKINGYKDGSFFTPGFITMYMCRETIRKAV
                     IQKFNSQDNDSSDLQSNRNDKFETIDDIYEAIGRKEFTRKQANEIINSLKICDPAVGS
                     GHFLVSALNELIAIKNDLKILEDKTGKSLNRYEIEVVNDELIVTDEDGELFEYNPTNK
                     ESQRIQETLFHEKQAIIENCLFGVDINPNSVKICRLRLWIELLKNAYYIQDSVQTHCN
                     ASLAIPLELQTLPNIDINIKCGNSLISRYPLDANLKTALKSSKWSIESYRLAISTYRN
                     TNDKNVKREMEKLILQIKQDFTSEIRKNDPLKSRMDKLANELYNRFTGNFLFEPQEQY
                     SKKGTDLDKKRKKEQQKLETEIKELSKKIDEIKGNKIYENAFEWRFEFPEVLDNNGDF
                     VGFDVV"
BASE COUNT          306 a          244 c          231 g          414 t
ORIGIN      
        1 cacaacatca aagccaacaa aatcaccatt gttgtccagc acttcgggaa actcgaatcg
       61 ccattcaaaa gcattttcgt aaattttgtt accttttatt tcatcaattt tttttgaaag
      121 ctccttaatt tctgtttcca gcttttgctg ttctttttta cgctttttat caagatcggt
      181 gcccttttta ctgtattgtt cctgaggctc gaaaagaaaa ttaccagtga agcggttgta
      241 taattcattg gccaacttat ccatgcgaga ttttagagga tcatttttcc tgatttcaga
      301 agtaaaatcc tgcttgattt gaaggattaa cttctccatc tctcttttta catttttgtc
      361 atttgtattt ctataagttg atattgccaa acgataactt tcgatactcc atttactgct
      421 ttttaaggca gttttaaggt ttgcatctaa agggtagcgg ctaatcagcg agttgccaca
      481 tttaatgttg atgtcgatgt tgggtaatgt ttgcaattcc aatggaattg caagagacgc
      541 attacaatgc gtctgtacag aatcctgaat ataataggcg ttcttcagca gttcaatcca
      601 caaacgcagg cggcaaatct ttacggagtt ggggttaata tccaccccaa acaaacagtt
      661 ttcaataatg gcctgttttt cgtgaaacag ggtttcctgt atgcgctggc tttccttgtt
      721 tgtgggattg tattcgaata gttcaccgtc ttcgtcggta acaatcagct catcgtttac
      781 cacttctatc tcgtagcggt tgagcgattt gcctgtttta tcttcgagta ttttaaggtc
      841 gttcttaatc gcaataagct cattgagcgc cgaaacgaga aagtggcccg agcccacagc
      901 cgggtcgcaa atcttcagcg aattaatgat ttcgtttgcc tgtttccggg tgaattcttt
      961 tctgccaata gcttcataaa tatcatcgat cgtttcaaat ttgtcattcc tgttgctctg
     1021 taaatcagaa gaatcattgt cctgactgtt gaatttctga attaccgcct tacgaatggt
     1081 ttcgcggcac atgtacatgg taataaaacc gggggtaaag aatgagccgt ctttgtagcc
     1141 gttaattttc tcgaaaatca agcccagcac cgatgcattg atcagggttt tattg
//
LOCUS       DAOPUW010000661         1192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_138004_length_1192_cov_4.011434, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000661 DAOPUW010000000
VERSION     DAOPUW010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138004_length_1192_cov_4.011434"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PKJ43_09610"
     CDS             <1..240
                     /locus_tag="PKJ43_09610"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNZ72869.1"
                     /translation="AGLPVIRTSPDHGTAFDIAGENQASPQSFLEALYLAIDVCRNRK
                     IYKEISKNPLPKYEINLNGESDDIDLSALEEQQQY"
     gene            243..824
                     /gene="ruvA"
                     /locus_tag="PKJ43_09615"
     CDS             243..824
                     /gene="ruvA"
                     /locus_tag="PKJ43_09615"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNZ72870.1"
                     /translation="MIEYLNGKLVEIYPTHVIVEVNGVGYYVNISLNTFTSFTGRTEA
                     KVLIHEVIREDSHLLYGFFSSEERELFRMLISVSGVGASTGILFVSSLAVAEIKLAII
                     SADIEKLKSIKGVGLKTAQRIVVELKEKLAKEPVSNDIFSLPDNSNRLEALSALVTLG
                     FARKEAEKTVDAVLRKNPGITVEQIIKDALKQM"
     gene            840..>1192
                     /locus_tag="PKJ43_09620"
     CDS             840..>1192
                     /locus_tag="PKJ43_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72871.1"
                     /translation="MLKKKFIYITALVLGMFFLGSIHLYAQSTGAAPASAAADSSGSL
                     KYPFNDQGAFDYPDEVEEEPLYLNRPANIERKIEYDPISKQYVIYEKVGNIYYRMPKA
                     MGLKEFVKYDFDQSVR"
BASE COUNT          358 a          245 c          270 g          319 t
ORIGIN      
        1 gctggcttac cagtaatccg tacatctccc gaccatggta ctgcttttga tattgccgga
       61 gaaaatcaag cttcgccgca atcattcctc gaagcgcttt atcttgcaat tgatgtctgt
      121 cgaaatagaa aaatttacaa agaaatcagc aaaaacccac ttccgaagta cgaaatcaat
      181 ttgaacggag agtcagacga tattgattta tcggcacttg aagaacaaca acaatattga
      241 aaatgattga atacctgaac ggaaaacttg ttgaaatcta tcctacccat gtaattgtag
      301 aggtaaatgg agtgggctat tacgtcaata tttcactcaa cacatttact tcgttcacgg
      361 gccgaaccga ggcgaaagtc cttattcatg aagtaatccg tgaagactct catttgcttt
      421 atggcttttt cagcagcgaa gagcgtgagc ttttccgcat gttgatatcg gtttccggag
      481 tgggggcaag tacgggcatt ttgttcgttt cctcgcttgc agttgccgaa attaaactgg
      541 ccattatttc tgccgatatc gagaaactga aaagcataaa aggagtgggg ctgaaaaccg
      601 cacagcgaat tgtggttgag ttgaaagaaa aactggctaa agagccggta tcgaacgata
      661 ttttttcatt gccagacaat agcaaccgcc ttgaagcgtt atctgcattg gtgacccttg
      721 gtttcgccag gaaagaggct gaaaaaacag tggatgctgt gctaaggaaa aaccccggaa
      781 taacagttga acaaattatc aaggatgctc taaaacagat gtaatccgtg tttttactaa
      841 tgttgaagaa aaaatttatc tacataacag cgcttgtctt gggaatgttt ttcctggggt
      901 caatccattt gtatgctcaa tctacaggag ctgcgccggc tagtgcagct gccgactcgt
      961 cgggttcgct taaatacccg ttcaacgatc agggagcctt cgactatcct gacgaagtgg
     1021 aagaggagcc tctctacctg aaccgacctg ctaacatcga gcggaaaatt gaatacgatc
     1081 ccatctcaaa gcaatatgtt atctacgaaa aagtgggtaa tatatattat cgcatgccca
     1141 aggcaatggg gcttaaggag tttgtgaaat atgatttcga ccagtcggta cg
//
LOCUS       DAOPUW010000662         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_139025_length_1187_cov_2.300353, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000662 DAOPUW010000000
VERSION     DAOPUW010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139025_length_1187_cov_2.300353"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PKJ43_09625"
     CDS             <1..477
                     /locus_tag="PKJ43_09625"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNZ72872.1"
                     /translation="EIKKLTPGTTMEVLIPDFDGIEDLITLVIDAKPDIISHNLETVR
                     RLTPLVRSRAKYDVSLQVIKTIAASDVVAKSGIMVGLGETRDEIHETMDDLLTVGCKV
                     FTIGQYLQPTTSHLPVVEYVHPDVFEEYRQIGLQKGFKIVESAPLVRSSYHAERHV"
     gene            536..>1187
                     /gene="lipB"
                     /locus_tag="PKJ43_09630"
     CDS             536..>1187
                     /gene="lipB"
                     /locus_tag="PKJ43_09630"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNZ72873.1"
                     /translation="MNTEILFEDLGVSEYKKTWEYQELLMQKVIAQKLAKKTRPDAES
                     DNYLLFVEHPHVYTLGKSGDEQNLLLNYIQLQAKDATFFHTDRGGDITYHGPGQLVGY
                     PIFDLENFGIGLRKYIFLLEEAVINVIAEFGLHGSRDDKATGVWLDVGTSQARKICAI
                     GVKSSRFVTMHGFALNVNTDLSYFQHINPCGFTDKGVTSLEKELGGKQDFDKVKLLI"
BASE COUNT          342 a          213 c          287 g          345 t
ORIGIN      
        1 gaaataaaaa agctaacccc gggtactacc atggaagtgc ttattcctga ttttgatgga
       61 attgaggatc ttatcaccct ggtaatcgat gccaaacctg atattatttc gcataatctt
      121 gaaacagtgc gaaggctaac cccgcttgtt cgtagccgtg cgaagtacga tgtaagcttg
      181 caggttatta aaaccattgc cgcttcggat gtggttgcca aaagcggcat catggttggc
      241 ctgggtgaga cccgcgacga aatacatgaa accatggacg acctgctgac tgtcggttgt
      301 aaagttttta cgattggtca ataccttcag cccacaacat cgcatttacc ggttgtggaa
      361 tatgtgcatc ccgatgtgtt tgaagagtac cgtcaaattg gattacaaaa aggctttaaa
      421 atagtggaaa gtgctccgct ggtacgttcg agttaccatg ccgagcggca cgtgtaaggc
      481 atatttttgt tgcgaggtaa atatgctgga aaatctattg gtaaaataat ataacatgaa
      541 tacggagatt ctttttgaag accttggggt ttcggaatac aagaaaactt gggaatacca
      601 agagttattg atgcagaagg tgattgctca gaaactagct aagaaaaccc ggcccgatgc
      661 agagagcgat aattatttgc tttttgttga gcatcctcac gtgtatacct tggggaaaag
      721 tggtgatgaa caaaatctac ttttaaatta tattcagtta caggctaaag atgccacttt
      781 ttttcatacc gacaggggag gggatattac ttatcacggt cccgggcagt tggtgggtta
      841 tccgattttc gatcttgaga attttgggat aggattacgg aagtatattt ttttattgga
      901 ggaagcagta attaatgtta ttgcagaatt tggactccat ggttcacgcg acgataaggc
      961 cacaggtgtt tggcttgatg ttggtaccag tcaagcccga aaaatttgcg ctattggggt
     1021 aaaaagttcc cgctttgtga ccatgcacgg ttttgcactg aatgtgaata ccgatctttc
     1081 gtattttcaa cacatcaatc cttgcgggtt taccgacaaa ggagtaacct cgctggaaaa
     1141 agaacttgga ggaaaacaag attttgataa agttaagtta ttgattt
//
LOCUS       DAOPUW010000663         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_139081_length_1187_cov_1.927562, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000663 DAOPUW010000000
VERSION     DAOPUW010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139081_length_1187_cov_1.927562"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PKJ43_09635"
     CDS             complement(<1..677)
                     /locus_tag="PKJ43_09635"
                     /inference="COORDINATES: protein motif:HMM:NF014923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNZ72874.1"
                     /translation="MSSQKYVMVAKTFAGLENVLMEELIEIGASDVEVLIRGVKFSGS
                     KEMLYKANFCCRTALRILVIIKEFKVKNANDLYNGVRSVEWPDYFDIQQTFAITSTAN
                     SEAFNNSMFVSLKTKDAIVDNFRAKFGKRPSVNAENPEIRINVHASSDEVSLSLDSSG
                     ESLHKRGYRVGQNEASMSEVLAAGILKIAGWKGQCDFYDPMCGSGTLPIEAALIARNI
                     PPGIFRKA"
     gene            complement(683..>1187)
                     /locus_tag="PKJ43_09640"
     CDS             complement(683..>1187)
                     /locus_tag="PKJ43_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HNZ72875.1"
                     /translation="AHGRGEVIFCYPAIRAISSYRIAHKLVELQVPLIPRIISEMAHS
                     ETGIDINPEAQIGEYFTIDHGTGTVIGATCIIGKNVKLYQGVTLGAVSFPLDNDGNPI
                     KGIPRHPIIEDDVIIYAQATILGRVTIGKGSVIGGNVWVTNDVAPNSKVVQLKVRSVS
                     YKDGGGI"
BASE COUNT          309 a          275 c          233 g          370 t
ORIGIN      
        1 gcttttctga atattcctgg cggtatgttg cgtgcaataa gggctgcttc gattggtaat
       61 gttcctgagc cacacatggg atcgtagaaa tcacattgtc ctttccatcc tgcaattttt
      121 aaaataccgg cagcaagtac ttcgctcatc gatgcttcat tttggccaac acggtagccc
      181 ctcttgtgaa gcgattcgcc tgaactgtcg agcgataaac ttacctcgtc ggacgatgca
      241 tgaacattga tgcgaatttc agggttttcg gcattaaccg atggcctttt gccaaatttt
      301 gccctgaaat tgtccacgat ggcatctttg gttttcaggc tgacaaacat cgaattattg
      361 aaagcttcgc tgtttgcagt actggttatc gcgaaagttt gctgaatgtc gaaataatca
      421 ggccattcca cacttcgcac gccattatat aagtcattcg cattttttac tttaaattct
      481 ttgataatga ctaaaatacg aagtgctgtc cggcaacaga aatttgcttt ataaagcatt
      541 tcttttgaac cagaaaactt taccccgcga ataagaactt cgacatcgga agcaccaatc
      601 tcgatcaatt cttccattaa aacattttcg aggccggcaa atgtctttgc caccattaca
      661 tacttttgtg aactcatgtt agttaaattc cccctccatc tttatagctt acgcttctta
      721 cttttaattg cacaactttt gaatttggag ctacatcgtt ggttacccat acgttaccac
      781 caataaccga gcctttccca atggttaccc ggccaagaat tgttgcctgt gcatatatta
      841 ttacatcgtc ttcaattata ggatgacggg gaattccctt tatcgggttt ccgtcattat
      901 cgagcggaaa acttaccgcg cccaaggtaa ccccctgata tagttttaca ttctttccaa
      961 tgatacatgt ggcaccgatt acagtacctg tcccgtggtc aatagtaaag tattcaccaa
     1021 tttgggcttc agggttaatg tcgatacctg tttcagaatg agccatttcg gaaataatcc
     1081 gagggattag cggcacctga agttcaacca gtttgtgtgc gattcggtaa ctggatatag
     1141 ccctaattgc ggggtagcag aaaatgactt ctcctcggcc atgagct
//
LOCUS       DAOPUW010000664         1183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_139715_length_1183_cov_3.916667, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000664 DAOPUW010000000
VERSION     DAOPUW010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139715_length_1183_cov_3.916667"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PKJ43_09645"
     CDS             <1..271
                     /locus_tag="PKJ43_09645"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNZ72876.1"
                     /translation="GVEARETRLGYIQRGGSPTTMDRILATRYGSYAVEIIAEGKYGK
                     MVAMKDGHLTTIPLNEVENKLNLVSTDHPLVKMCRNMGVSFGDEY"
     gene            443..>1183
                     /locus_tag="PKJ43_09650"
     CDS             443..>1183
                     /locus_tag="PKJ43_09650"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNZ72877.1"
                     /translation="MKRLLTLGALLFSFIYLFAQPKMEFKSIERDFGTIKEDAGLATT
                     VFEFKNKGDQPLIINNATATCGCTTPEWTKEPIAPGKSGSIKVSYNPKNRPGQFNKNV
                     NVYSNADPSMAVLTIKGKVEPRQLSIEEQYPREMGGIRWKTNYLTLGTVLNSQEKTDT
                     LAYYNSSSKDVKVGIYRVPAYMTVSFEPEVVPAGKYGKMTVKYDAKRRNAFGNVSDRI
                     YLDIDGQKDNNFSIGVSVTINEDFGSLTE"
BASE COUNT          376 a          220 c          245 g          342 t
ORIGIN      
        1 cggtgttgag gcccgggaaa caaggctggg atatattcag cgcggaggca gtcctaccac
       61 catggacagg attttggcta cccgctatgg ttcgtatgct gtggagataa ttgccgaagg
      121 aaaatatgga aagatggtcg ccatgaaaga cggacatttg acaaccatcc cactcaacga
      181 ggtggaaaac aagctcaatt tggtttcaac tgaccatcct ttggtgaaaa tgtgccgaaa
      241 catgggcgta tcttttggcg atgaatatta atcaaaagat attgtttctc aattggtctt
      301 aatttttatc gggtatgata tattttttga agtcattcgt cttgttttcg atatgaatga
      361 aaatcatctt tggaacagat tttgatttta gataaccatt cattatttct aattttgtaa
      421 aatgtaaaaa aacaataaga ttatgaagcg tttactaaca ttgggagcat tattgttctc
      481 gtttatttat ttgtttgctc aaccgaaaat ggaattcaaa tcgattgaac gcgactttgg
      541 aaccataaaa gaagatgcag ggcttgccac tactgtcttc gaatttaaaa acaagggtga
      601 ccaaccactt atcattaaca atgcaacagc tacttgtggt tgtactactc ccgaatggac
      661 caaagaacct attgccccgg ggaaatcagg ttcgataaaa gtatcgtaca acccaaagaa
      721 caggcccgga cagttcaata aaaatgttaa tgtgtattct aatgccgatc cttctatggc
      781 agtactaacc ataaaaggaa aagttgaacc tcgacagctc tcaattgaag aacagtatcc
      841 acgcgaaatg ggtggtattc gttggaaaac caactaccta acccttggaa cagtgctcaa
      901 ctctcaggaa aaaacagata ctcttgcata ttacaattca agctcaaaag atgtgaaagt
      961 tggcatttat cgtgtgcctg cttatatgac tgtttcattt gagccagagg tggttcctgc
     1021 tggtaaatat ggaaaaatga ctgttaaata tgatgctaaa cgccgtaatg ctttcggaaa
     1081 tgttagcgac cgtatttatc tcgatattga cggacagaaa gataacaact tctcgatagg
     1141 tgtttctgtc accataaacg aagactttgg ctcacttact gaa
//
LOCUS       DAOPUW010000665         1183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_139719_length_1183_cov_3.719858, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000665 DAOPUW010000000
VERSION     DAOPUW010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139719_length_1183_cov_3.719858"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PKJ43_09655"
     CDS             <1..490
                     /locus_tag="PKJ43_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNZ72878.1"
                     /translation="YNVPSRCGVNMSAETTLRLANSSSKFIAIKEASGNIDQIKKIID
                     ESPVGFGVISGDDSMIYPINALGGDGVISVMANALPAETVMLTNNSMNNEPIAEAEQD
                     GYNELIKLLFVEGNPAGVKAILHQKGMIDNVLRLPLCPVSDKVYQAISEELMSIGNVN
                     HF"
     gene            474..>1183
                     /locus_tag="PKJ43_09660"
     CDS             474..>1183
                     /locus_tag="PKJ43_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HNZ72879.1"
                     /translation="MSIIFNQIPSPCFVLDEHLLEKNLALIASVKQRAGIDIILAFKG
                     FAMWGVFPLVKKYIQSTTASSLNEALLAVREFGTPAHTYAPVYSDEDFPGILDCSSHI
                     TFNSLSQFEKFLPVIQQSGREVSVGLRVNPEYSEVETELYNPCAPGSRLGVLASQLPE
                     KLPEGIDGLHFHALCESKSFDLEKTLQAFENKFSRYFSQIKWVNMGGGHLMTHKDYDV
                     EHLISVLKNFRERTGLEV"
BASE COUNT          325 a          243 c          268 g          347 t
ORIGIN      
        1 gtacaatgtg ccttcgcgtt gcggggtaaa catgagtgcc gaaacgacct tgagacttgc
       61 aaattcttct tcaaaattca ttgcgataaa agaagcttca ggaaacattg accaaatcaa
      121 aaagattata gatgaatctc ctgtcggatt tggggtaatt tcgggcgacg attcaatgat
      181 ttatcctatt aatgcgctcg gtggtgatgg ggttatttcg gtaatggcca atgcattacc
      241 tgccgaaact gtgatgctta ccaacaacag catgaataac gaacctattg ccgaagccga
      301 acaagatggc tacaacgaac tcattaaatt gctttttgtt gaaggaaatc ctgccggggt
      361 taaagctatt cttcaccaga aaggaatgat agataatgta ttacgtttac cgttgtgccc
      421 tgttagcgat aaagtgtatc aagcaatttc tgaagaattg atgagtatag gaaatgtcaa
      481 tcatttttaa ccaaattcct tcgccctgct ttgttctcga tgaacatctg ctcgagaaga
      541 acctcgcgct tattgcttcg gtaaaacaac gggctggtat tgacattata ttggcattca
      601 aaggttttgc catgtggggc gtgttccctt tggtgaaaaa atacatacaa tcaactactg
      661 ccagttcgct caacgaagcc cttttggccg ttcgtgagtt tggtacgccg gcacacacat
      721 atgccccggt gtattccgat gaagatttcc ctggcatttt agactgctcc agccatataa
      781 ctttcaactc gctcagccag ttcgaaaaat tcttgccggt aattcagcag tcgggcaggg
      841 aagtgtcggt tggcttgcgt gttaaccctg aatattccga agtagaaaca gaactctata
      901 atccttgtgc accgggatcc cgccttggtg tgcttgcttc tcagttgccc gagaagttac
      961 ctgaggggat tgatgggctt cacttccatg cattgtgcga gtcgaagtcg ttcgatctgg
     1021 aaaagacact acaagctttt gaaaataaat ttagtcgata tttttctcaa attaagtggg
     1081 ttaacatggg tggaggacat ttaatgaccc ataaagatta cgatgttgaa catcttattt
     1141 ccgtgttgaa aaactttcgt gaacgtaccg gccttgaggt tat
//
LOCUS       DAOPUW010000666         1176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_141173_length_1176_cov_2.022302, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000666 DAOPUW010000000
VERSION     DAOPUW010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141173_length_1176_cov_2.022302"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PKJ43_09665"
     CDS             complement(<1..535)
                     /locus_tag="PKJ43_09665"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HNZ72880.1"
                     /translation="MKIHKEGLVSLFVVAAFILIVDYIVLQLTNEKLIVYPLLIASLL
                     LYLFVRHFFRVPNRQSIEDVNGIISPCDGKIVVIEDVFEKDFLQCECKQISIFMSPLD
                     VHINWFPVGGNILHASRTKGSHLVAWAPKSSTHNERSTVLIETADNKKILVRQIAGAV
                     ARRVVCYAKEGSYAQQNG"
     gene            complement(542..>1176)
                     /locus_tag="PKJ43_09670"
     CDS             complement(542..>1176)
                     /locus_tag="PKJ43_09670"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNZ72881.1"
                     /translation="LLFYAGMFLLQYNLFTFQKIFPFGLLVLLIPFAELTRKKKEPIE
                     NMALTFVGIMLTAIPFSFFNLIPNPLNNESGAYNPWLMIGVFVIIWTNDTGAYLIGSM
                     IGKNKLAEKISPNKTWEGAIGGAVFAILFAILYFHFTNLFTPVETVFISLATVIAGTM
                     GDLTESLIKRNFNVKDSGNMLPGHGGLLDRFDSLLFAAPVYYVMIISFLK"
BASE COUNT          384 a          212 c          241 g          339 t
ORIGIN      
        1 gtccgttttg ttgggcatag gagccttctt tggcatagca aactacacga cgggcaactg
       61 cccctgctat ttggcgtact aatatttttt tgttatcggc agtttcaatt aagactgtcg
      121 aacgttcgtt gtgtgttgat gattttggag cccaagcgac caaatggcta ccttttgtgc
      181 gcgaagcatg aagaatgttc ccacccacag gaaaccaatt gatgtgaaca tcgagtggtg
      241 acatgaagat tgatatttgt ttacactcac attggaggaa atctttctca aatacatctt
      301 caattactac aatctttccg tcgcaaggag atatgatgcc atttacatcc tcaattgatt
      361 gtcgattggg caccctaaaa aagtgacgaa caaagagata aagtaacagt gaagcaataa
      421 gtaatggata tacgattagt ttctcgtttg ttaattgtaa aacaatgtaa tcaactatta
      481 atatgaatgc tgcaactaca aaaagtgaaa cgagaccttc tttgtgaatt ttcataatca
      541 attattttaa gaagcttata atcattacat agtataccgg agcagcgaaa agcaggctat
      601 cgaaccggtc gagaagccct ccatgtccgg ggagcatatt gcccgaatct tttacgttga
      661 aatttcgttt aatcagagac tcggttaagt cgcccatcgt acctgcaatt actgttgcaa
      721 gagagataaa aacagtttcg acaggcgtga ataaatttgt aaaatggaaa tagagaatgg
      781 caaagaggat ggcaaataca gctccaccaa tagctccttc ccatgtttta tttggagaga
      841 ttttctctgc aagtttgttt tttccaatca tactaccaat taaataagct ccagtgtcgt
      901 tagtccaaat aatgacaaaa acgccaatca ttagccatgg attgtatgcc cctgattcat
      961 tgtttagagg gttaggaata agattgaaaa aggagaacgg aatagcggtg agcataatgc
     1021 caacaaaggt aagtgccatg ttttcaatgg gttctttctt ttttctggtt agctcagcaa
     1081 aagggataag tagaactaaa agcccaaagg gaaatatttt ttgaaaagta aaaagattgt
     1141 attgaagcag aaacatacct gcataaaaaa gcagcc
//
LOCUS       DAOPUW010000667         1175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_141294_length_1175_cov_2.778571, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000667 DAOPUW010000000
VERSION     DAOPUW010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141294_length_1175_cov_2.778571"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="PKJ43_09675"
     CDS             complement(<1..1086)
                     /locus_tag="PKJ43_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72882.1"
                     /translation="MFTHSITQNDALQRRQQLVAELSSDTEWRHDFAALGMMYAEDKE
                     ESEMFGRWAKETFSFSMSKAIPVLLVVMPLFAILSLIYWIVFSNSALFLFSILLQSIL
                     WMVERKNTTLIYARFGRRVKILSKYATLLSRIEQRNWKSDEAISILASMETRGIPSKE
                     ISQLKQLVSAYDNRNNFLLGFMLNVVFMWDILCSYRLANWHIRNKENYTLWDSTIGFF
                     DAIASLSTLAYNHPGYCYPVFSTGKFMVEANQLGHPLIKPDKMIANNFAMKGEKQLVI
                     VTGANMAGKSTFLRTIGVNLVLAMTGAPVCAQRMEFVPVEVFSNMRTTDSLFDEESYF
                     FAELKRIKMILDALSSGRKVLIILDEIL"
BASE COUNT          385 a          266 c          219 g          305 t
ORIGIN      
        1 caatatttca tcaagaatga tgagcacctt acggcctgaa ctcaacgcgt cgagaatcat
       61 cttaatccgc tttaattcag caaagaaata cgattcttca tcaaaaagtg aatcggttgt
      121 acgcatgttt gaaaagactt caaccggaac aaactccatt cgctgtgcac aaacaggagc
      181 accggtcatg gccaacacca aattcacccc aatagttctg agaaaagtgc ttttccccgc
      241 catattggcc ccggtcacaa tcaccagctg cttctcgcct ttcatcgcaa aattgttggc
      301 aatcattttg tcaggcttga tgagcggatg cccaagctga ttggcttcca ccataaactt
      361 accggtacta aataccggat aacaatatcc cgggtgatta taggctaatg ttgaaagtga
      421 cgcaattgca tcgaaaaaac caatggttga atcccacaat gtatagttct ctttattgcg
      481 tatatgccaa ttagccaacc ggtaagagca aagaatgtcc cacataaaaa ccacattcaa
      541 cataaagccc aataaaaaat tgttccgatt atcgtaggca gaaacaagtt gtttcaattg
      601 tgatatttct tttgaaggga tgccccgggt ttccatggat gctagtattg aaattgcctc
      661 atccgatttc caatttcgtt gttcaatacg acttaataaa gtggcatact ttgaaagaat
      721 tttaaccctg cgcccaaaac gtgcataaat taacgtcgtg tttttccgtt caaccatcca
      781 aaggatcgat tgaagcagga tagaaaagag aaataaagcc gagttggaaa aaacaatcca
      841 gtaaatcaac gaaagaattg caaataaagg catcacaaca agaagcacag gaatggcttt
      901 cgacattgaa aacgaaaagg tttctttggc ccagcgtcca aacatttcgc tttcttcttt
      961 atcctcggca tacatcattc ccaaggcagc aaaatcgtgg cgccactcag tatcggacga
     1021 gagttcagca accagttgct gccttctttg taacgcgtca ttttgagtaa ttgaatgggt
     1081 aaacataccg gctaaaaccg atttaccccg gaaagtaaca gttctgttaa tatactgaaa
     1141 aagagaacct ttaccaaaca catcaaggtc atatg
//
LOCUS       DAOPUW010000668         1175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_141386_length_1175_cov_1.700000, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000668 DAOPUW010000000
VERSION     DAOPUW010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141386_length_1175_cov_1.700000"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PKJ43_09680"
     CDS             <1..419
                     /locus_tag="PKJ43_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72883.1"
                     /translation="DPENYEANGKLCIIKEVTTIKETFDIMKSVVPMLDTIFYISDNL
                     KSGSLLMKEAEDIVLTKFMGITIKFITCIDNKTLGERCRVSTIQAVFFAHLLASTATG
                     NLLINSKLHKLLENMQKHLFSRVIMPLEPMAISGGT"
     gene            434..>1175
                     /locus_tag="PKJ43_09685"
     CDS             434..>1175
                     /locus_tag="PKJ43_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNZ72884.1"
                     /translation="MGKLTAETALKILTNNNQPVEKLIIPQSETVFDFRSLEKHNIPE
                     HLLPAHAIVLNKPVTIWERSKKVILISLAIIINLLIVILLLTKTVFAQKKHKSQLLVA
                     MEKANESDRLKTTFLENFSHEMRTALNSIVGFSDVLLEMNTDPTLNDYVKIISENAIT
                     LNYTINHIFDFSLIKTHNIDFNLSKVNLNQLLDRAFETIAEEKRYSESPVKLFIEPDI
                     QNRDTIVESDENKLYQVLRHLIDNAFKFT"
BASE COUNT          411 a          253 c          198 g          313 t
ORIGIN      
        1 gtgacccgga aaactacgaa gccaatggca agctatgcat aataaaagaa gtaacaacca
       61 ttaaagagac cttcgatatt atgaaaagcg ttgttcccat gctcgatact atcttttata
      121 tttctgacaa tttaaaaagc ggctcactcc tcatgaaaga agccgaagat attgtattga
      181 ctaaatttat gggaattaca ataaaattta ttacctgtat cgacaataaa accttagggg
      241 aacggtgtcg cgtatccact atccaagctg tattttttgc tcatctgtta gcatcgactg
      301 caacgggcaa cctgttgatt aactcgaaat tgcacaaatt gttggaaaat atgcaaaagc
      361 acctcttttc tcgggttatt atgccactcg aaccgatggc tatatcgggg ggaacataac
      421 aaccggcgaa caaatgggaa aacttacagc cgaaactgcc ctgaaaatac taacaaataa
      481 taatcaaccc gttgaaaagc taatcattcc ccaatcagaa actgtctttg atttcaggag
      541 ccttgaaaag cataatatcc ctgagcactt gctaccggca cacgccatcg tgcttaacaa
      601 accagttacc atttgggaaa gaagcaagaa agttatcctg atttcgctgg caatcatcat
      661 caatctactg attgttatac tccttctcac aaaaacagtc tttgctcaaa aaaaacacaa
      721 aagccagcta ttggtcgcaa tggaaaaagc aaacgaatcg gaccggctaa aaacaacttt
      781 cctcgagaat ttttcgcacg agatgcgcac cgcactaaat agtattgtgg gtttttcaga
      841 tgttctgctc gaaatgaata ctgaccctac actcaatgat tatgtgaaaa ttatctctga
      901 gaatgccatt accttgaatt atacaataaa ccacattttt gatttctctc tcataaaaac
      961 acataatatc gatttcaacc tctcaaaggt taatctaaac caattgttag acagggcttt
     1021 tgaaaccatc gctgaagaaa agcgatattc cgaaagtcca gtaaaattat ttattgaacc
     1081 tgacatccaa aatagggata ctattgtcga aagcgacgaa aacaaactct atcaagtatt
     1141 gaggcacctg atagacaacg cattcaaatt cactc
//
LOCUS       DAOPUW010000669         1174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_141487_length_1174_cov_2.498660, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000669 DAOPUW010000000
VERSION     DAOPUW010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141487_length_1174_cov_2.498660"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PKJ43_09690"
     CDS             complement(<1..250)
                     /locus_tag="PKJ43_09690"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNZ72885.1"
                     /translation="MKIIIVGAGEVGTHLAKLLARESMEITLSDENPDRLMDLESNYD
                     LLTSVGSSTSINHLREIGVENANLFIAVTPYESVNMTAC"
     gene            complement(247..>1174)
                     /locus_tag="PKJ43_09695"
     CDS             complement(247..>1174)
                     /locus_tag="PKJ43_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNZ72886.1"
                     /translation="KEFPDRVFDVGIAEGHAVTFSAGLAKEGMIPFCNIYSTFCQRAY
                     DNVIHDVALQNLHVVICLDRAGLVGADGPTHHGVFDLAYMRCIPNMTIAAPRNEIELR
                     NLMFTAQQPDLGPFVIRYPRGAGSVVDWRKPMQRLEVGKGECLKEGENLAVLTIGTMA
                     VKAEKAIAKVENECHLSIAHYDMRFLKPLDENMLHKIGAKFSYVLTIEDGVIQGGLGS
                     AVLEFFADHNYSVRVKRLGIPDNFIEQGSPDELYAMLDMDENGISKTIIDMLQKNTEN
                     DNPKKQQIVSSNNFGKNKNSIINRIFRKDFKE"
BASE COUNT          336 a          268 c          203 g          367 t
ORIGIN      
        1 tacaggctgt catgtttaca ctttcatagg gagtaacagc tataaacaaa ttggcatttt
       61 ctacgccaat ttccctcaag tgattgatgg aagtggaaga acccacactg gtgagtaaat
      121 cgtagttgct ttccaaatcc atcagccggt caggattttc atcacttagg gttatttcca
      181 tactttcccg agccagcaat tttgctaagt gcgttccgac ttctcctgcc ccaacaataa
      241 taattttcat tctttgaaat cttttctgaa aattctgttg attatagaat ttttattttt
      301 tccaaaatta ttggaagaaa ctatctgttg tttttttgga ttatcgtttt cagtgttttt
      361 ttgtagcatg tcgataatgg tttttgatat cccattttcg tccatatcca gcatggcata
      421 taattcgtca ggcgaacctt gttcaataaa attgtcaggt atgccaagtc ttttcacccg
      481 aaccgaataa ttatgatcgg caaaaaattc cagcactgca ctgccaagcc ccccttgtat
      541 aacgccatcc tcaatagtca aaacgtatga aaattttgct ccgattttat ggagcatatt
      601 ttcatccaaa ggtttaagaa aacgcatgtc ataatgtgca attgacaaat ggcactcgtt
      661 ttcaactttc gcaatagctt tttcggcttt cactgccatt gttccaatag ttaaaacagc
      721 caaattttca ccctctttca aacattcacc tttacctact tcaagtctct gcattggttt
      781 tctccaatcg acaacagatc cggctccacg aggatagcga attacaaacg gtcccaagtc
      841 gggttgttgc gccgtaaaca tcaaattccg caattcaatc tcatttcgag gagcggcaat
      901 ggtcatattg ggaatacaac gcatataggc caaatcgaat acaccgtgat gagtggggcc
      961 atcggcaccc accaatcccg cacgatcgag acaaatcact acatgcaaat tttgtaatgc
     1021 tacatcgtga atcacattgt cataagctcg ttggcaaaaa gtcgaataaa tgttacagaa
     1081 aggaatcatt ccttctttcg ccaatccggc cgaaaaagtt acggcatggc cttcggctat
     1141 acctacatca aatactctgt cgggaaattc tttt
//
LOCUS       DAOPUW010000670         1173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_141650_length_1173_cov_2.964222, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000670 DAOPUW010000000
VERSION     DAOPUW010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141650_length_1173_cov_2.964222"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1173
                     /locus_tag="PKJ43_09700"
     CDS             <1..>1173
                     /locus_tag="PKJ43_09700"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HNZ72887.1"
                     /translation="SSGILLPEYGEEQNRGFFLRNGGFYLGLNDYFDLGVTGDIYTKG
                     SWAVNTRSNYRKRYRYSGNFSIAISENVFGEKGQDDYIKNRSYWLRWSHSQDPKASPN
                     STFQASVNLGSPNHNRYNARSVDAFLTNSISSSISYSKNWPGTPFSLSASMNHSQNNL
                     DSTISLGLPKVSFNMSRIYPLKKKDKIGNTTWYENIGLSLSTRFDNSISTKTNKLFTP
                     ETLREFKNGAQHNIPVSTSFNILKYFIISPSVNYTENWYTKTIQKRWDPDSVYVQSGD
                     TIRGRVVTDEILGFARAWQYSTGVSMNTKIYGMFRFGQNSPVQAIRHVMTPSVGLSYR
                     PDFSREHYGFYKTVQSDSTGNKFETYSIFEQSLFGGPGSGKSGLLNLSLSNNLEMKV"
BASE COUNT          354 a          258 c          247 g          314 t
ORIGIN      
        1 atcgtcaggt attctgcttc cggagtacgg cgaagaacag aaccggggat tttttttaag
       61 gaatggaggt ttttaccttg ggcttaacga ctacttcgat cttggcgtaa cgggcgatat
      121 ttataccaaa ggttcgtggg ctgtcaatac ccggtcaaac taccgtaaac gctatcgtta
      181 ttcgggtaat ttttccatcg ccatatcgga gaacgtgttc ggagaaaaag gtcaggatga
      241 ctatattaaa aacaggtctt actggctccg atggagccac tcacaagacc caaaggccag
      301 tccaaactcc acatttcagg cttcggtaaa cttgggatct ccaaaccata ataggtacaa
      361 cgcccgaagc gtagatgcat ttttaaccaa cagcatttcg tccagcattt cctactcaaa
      421 gaattggccg ggtacgccat tcagcttatc cgcctccatg aaccattcac aaaacaattt
      481 ggatagcact atttctcttg gattgccaaa agtttcattc aatatgagcc gtatttaccc
      541 tttaaagaaa aaggataaaa ttggcaacac tacgtggtat gagaatattg gattatcgct
      601 atctacacga ttcgacaatt caatcagtac caaaaccaac aaacttttca cccctgaaac
      661 tctgagagag tttaaaaatg gggcacagca taatatcccg gtttccactt catttaatat
      721 tttgaaatat ttcataataa gcccatcggt caattacacc gaaaattggt acactaaaac
      781 catacaaaaa cgctgggatc ccgactctgt ttacgttcaa tcgggtgata ccattcgtgg
      841 tagagtagtt acagatgaga ttctaggttt tgcccgagcc tggcagtata gcacaggcgt
      901 atcgatgaat accaagatat acgggatgtt taggtttggc caaaactcac cggttcaggc
      961 aatcaggcat gtaatgacac cgtcggtggg actatcgtac cgcccggatt ttagccggga
     1021 gcattacggt ttttacaaaa ccgttcaaag cgacagcaca ggaaataaat ttgaaactta
     1081 ctctattttt gagcaatcgt tgtttggagg ccccggatcg gggaaaagtg gattgttgaa
     1141 cctatctctt agcaacaacc tggaaatgaa agt
//
LOCUS       DAOPUW010000671         1171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_142103_length_1171_cov_2.016129, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000671 DAOPUW010000000
VERSION     DAOPUW010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142103_length_1171_cov_2.016129"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="PKJ43_09705"
     CDS             complement(<1..962)
                     /locus_tag="PKJ43_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNZ72888.1"
                     /translation="MAKRDDIKKVLIIGSGPIIIGQACEFDYSGTQACKALRSMGYEI
                     VLVNSNPATIMTDPGMADKTYIEPLNEFALTEIIKKERPDALLPNLGGQTALNLSLQL
                     AKKGILDQYGVKVIGVNIDAIERGEDRVAFKETMKKLGVDMPKSTAVNTVEDAEKIAA
                     ELGYPVVVRPAYTMGGTGGGLVYNVEELRTIASRGLAASIVTQVLIEESVLGWEELEL
                     EVVRDSKGKMITVCFIENVDAMGVHTGDSYCTSPMLTISTEVQKRLQKHAYDIVEAIE
                     VIGGTNVQFAHNPNTGRIVVIEINPRTSRSSALASKATGFPIALV"
BASE COUNT          299 a          247 c          233 g          392 t
ORIGIN      
        1 accagcgcga taggaaagcc tgtagctttc gatgccaaag ccgatgagcg tgaggttcgt
       61 ggattaattt caattacaac aatgcggcca gtattgggat tatgggcaaa ctgaacattt
      121 gtaccgccaa ttacttcaat ggcttctaca atatcataag catgcttttg gagccgtttt
      181 tgaacctctg tgctaatggt aagcattgga gaagtacaat acgagtcgcc ggtgtgaacc
      241 cccatggcat caacattttc gatgaagcaa acggtaatca tttttccttt tgaatcacga
      301 acgacttcaa gttcgagttc ttcccatcca agtaccgatt cttcgataag gacttgtgtt
      361 acaatgcttg ctgctaaccc tcgactagca attgtacgca gttcttccac gttgtacacc
      421 aaaccacctc cggtgccgcc cattgtatat gcaggcctga ctaccaccgg gtaacctaat
      481 tcagcagcaa ttttttcagc atcttcaaca gtgtttaccg ctgtactttt gggcatatca
      541 acgcccaact ttttcattgt ttctttaaaa gcaacacggt cttcgcctcg ttcaattgca
      601 tcgatattta ctccaattac ctttactccg tattggtcga ggattccttt tttcgctaat
      661 tgtagggaga ggttaagagc tgtttgccct ccaagatttg ggagtaacgc atcgggtcgt
      721 tctttcttaa ttatttcggt gagcgcaaat tcattgagtg gctcaatgta cgttttgtca
      781 gccattcctg ggtcggtcat gatagtggcc ggatttgaat tgaccagcac aatttcgtac
      841 cccatgctcc gaagggcttt gcacgcttga gttcctgaat aatcgaactc gcaagcctgt
      901 ccaatgatga ttgggcctga tcctataatc aggacttttt taatgtcgtc tcgttttgcc
      961 attgtgagtg ttgaataata tagaaatggt tattatcaat tttttatgaa atggttcaat
     1021 cctgttgaat tttttcagtt ttcagggttc aaaaatagga atttatatgc cctagcttgt
     1081 actattcaaa aataatattt caacagtttt ttatgatatt attgacaaaa ttcagttttt
     1141 tactgatttg agttaagata attgcttttt t
//
LOCUS       DAOPUW010000672         1168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_142610_length_1168_cov_2.782570, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000672 DAOPUW010000000
VERSION     DAOPUW010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142610_length_1168_cov_2.782570"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /gene="sprA"
                     /locus_tag="PKJ43_09710"
     CDS             complement(<1..1151)
                     /gene="sprA"
                     /locus_tag="PKJ43_09710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNZ72889.1"
                     /translation="MIKKTMQSLVDRGFEADFYWQKVDQARRLNAGEYTLNKELGFIS
                     LNSALNNDEVLAVAYNITIGDTTFQVGEFSTDSENSKQTLVLKLLKGTTLSPNYPTWD
                     LMMKNVYNIGAYDLSGDDFEFHVVYKNDSTTTLLTTLPVEEMNNTTLLRVMNLDNLNS
                     QLDYTKGGDGLFDFIEGVTVIKSSGRIIFPVLEPFGSNIANKLSSQANKDKYAFTSLY
                     TKTKVDAQRSDVNHDKFYMVGSFKGSSGSEISLNTFNLAPGSVIVYAGSTKLVENVDY
                     TVDYAMGKVKIINESYISAGTPIKVSTESQDLISMQRKTLVGTYGSYAFSDNLNIGGT
                     FMYMNERPMTNKVDVGEEPVSNMMLGLDFQYRKQSQLLTDVVNLLPFYES"
BASE COUNT          333 a          250 c          233 g          352 t
ORIGIN      
        1 gattcgtaga aaggcaaaag atttaccacg tcggtaagca actgagactg cttgcgatat
       61 tgaaaatcaa gaccaagcat catattagac acaggctctt cgcccacatc gactttgttg
      121 gtcatagggc gttcgttcat gtacatgaaa gttccaccga tgttcagatt atcggaaaat
      181 gcataactcc cataagttcc aacgagggtc tttcgctgca ttgaaattaa atcctgactc
      241 tcggttgaca cctttatagg cgttccggca ctaatgtatg attcgttgat aatcttcacc
      301 ttgcccatgg cataatcgac tgtataatcg acattttcta ccaacttcgt gcttccggca
      361 tatacaatga cagatccggg agccaaattg aatgtattca gtgaaatttc tgagcccgat
      421 gaacctttga acgaaccaac catgtaaaat ttatcatgat tcacatctga tcgctgggca
      481 tcgaccttcg ttttggtata gagcgaggtg aatgcatact tgtctttatt tgcctgagag
      541 gataatttgt tggctatgtt tgaaccaaac ggctctagta ccggaaaaat aattcgcccg
      601 gaacttttta ttacggttac cccttcgatg aaatcgaata atccatcgcc acctttcgtg
      661 taatcgagtt gtgagttcag gttatcgagg ttcattaccc gcaaaagagt ggtgttgttc
      721 atttcttcaa caggtaatgt tgttaaaagc gttgtagttg agtcgttttt atacacaaca
      781 tgaaattcga aatcatcacc cgaaagatca tacgcaccaa tgttatacac gtttttcatc
      841 atcaaatccc aggtaggata attgggcgac aacgttgtgc ctttcagaag tttaagaaca
      901 agagtttgct tcgaattttc actgtcggta gaaaattcac cgacctgaaa agtagtatct
      961 ccaatggtga tgttgtaggc aacagccaac acttcgtcgt tgttcaatgc tgaattgaga
     1021 gaaataaaac caagttcttt gttcaacgta tattcaccgg cattaagcct gcgggcctgg
     1081 tcaactttct gccagtaaaa atcagcttca aaaccacggt cgaccaacga ttgcatggtt
     1141 ttttttatca tttttgattc ccgaatcc
//
LOCUS       DAOPUW010000673         1157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_144875_length_1157_cov_2.242287, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000673 DAOPUW010000000
VERSION     DAOPUW010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144875_length_1157_cov_2.242287"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="PKJ43_09715"
     CDS             complement(<1..857)
                     /locus_tag="PKJ43_09715"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HNZ72890.1"
                     /translation="MFFRKLSILSITMLLISMSYSFSQPIKKVKFGKISMEEMTMQTY
                     SLDTTAEAVLLYQNAEFLPNNFQFRQHQRIKILKKSGTGYANIAFPGQLKSQIKGYTY
                     NLEDGKIVKTKLSKEAVFEERVVGNIYRTRIAMPNVKEGSVIEVEFTKQGIANSIEIQ
                     RTIPVMYSVVSLPQHPNIDFSIKVIGLLGPSYNQDDTWVFKDLPAFVREPYLLSDMDY
                     RVRFEIEIRTIQLANQYYQLFSTFASSWKAVTKSFNDDPYFGKKINYLSLYLNSLADS
                     IKSISSNDE"
     gene            complement(939..>1157)
                     /locus_tag="PKJ43_09720"
     CDS             complement(939..>1157)
                     /locus_tag="PKJ43_09720"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNZ72891.1"
                     /translation="QIGSYKELVNLLRIADDLKVSLKKEVRVQVATVGGEKVYRLMVG
                     KFSSRRDAENFRDKASQIYPDCFVVELK"
BASE COUNT          297 a          230 c          245 g          385 t
ORIGIN      
        1 tcctcatcgt tggaagatat actttttatg ctgtctgcca aactgttcag gtaaaggctt
       61 aaatagttaa tctttttgcc gaagtatgga tcgtcgttga atgattttgt tacagctttc
      121 catgacgagg caaacgttga aaatagctgg tagtattgat ttgccagttg gatagttctg
      181 atttctattt caaatctcac gcggtaatcc atatccgata gtaggtatgg ctctctaaca
      241 aaggcgggca aatctttgaa aacccaggtg tcatcctggt tatatgaagg acccaaaagt
      301 ccaatgacct taatggaaaa atcgatattc ggatgctggg gcaatgatac aacgctgtac
      361 ataacgggta ttgttcgttg aatttcaatg gaattggcta ttccttgctt tgtgaactca
      421 acttctataa ccgacccttc tttcacattg ggcatggcta tccttgtgcg ataaatgttc
      481 cccacaaccc gctcttcgaa aacggcctct ttgctcaatt tggtttttac aatcttgcca
      541 tcctccagat tataagtata tccttttatc tgtgatttta attgtcccgg aaaggcaatg
      601 ttggcgtatc ctgttcccga tttttttaaa attttaatcc gttggtgctg tctgaactga
      661 aaattattgg gcaggaattc ggcattttgg taaagcagta cagcctcagc tgttgtgtcg
      721 aggctatagg tttgcatagt catttcttcc atgcttattt ttccaaactt gactttttta
      781 atcggttggg aaaaggaata ggacatgcta attaaaagca tggtgatgga aaggatagat
      841 agttttctaa agaacatgtt tgaattgtat taaagggtta atgtttgttt ttattaaggt
      901 gcttgatttt cagttgatat tacaagttgt tgaggtagct attttaactc cacaacaaag
      961 cagtcggggt atatttgcga ggccttgtcg cggaagttct cggcatcgcg gcgtgacgag
     1021 aatttcccta ccatcagccg gtacaccttt tcgccaccta cggtggccac ttgtaccctc
     1081 acctctttct taagcgacac ctttaggtca tcggcaatcc gaagcaggtt gaccaactcc
     1141 ttgtaactgc caatctg
//
LOCUS       DAOPUW010000674         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_145016_length_1156_cov_2.766576, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000674 DAOPUW010000000
VERSION     DAOPUW010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145016_length_1156_cov_2.766576"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..925)
                     /locus_tag="PKJ43_09725"
     CDS             complement(86..925)
                     /locus_tag="PKJ43_09725"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ72892.1"
                     /translation="MNRSYLSIKNLICGYGNFSLDSLNFEIGKGVFAGVIGPNGSGKT
                     TLMKAITGELKISKGLIMLDNKNLNQLSIREKATKIAIVTQHTEEVDISVEDYVLMGR
                     MPYRTNFQFFETKEDLSIAEKYMHLTDVFRFKDKLMSKLSGGERQLAGIARALTQEPQ
                     LLLLDEPTSQLDISHQIQVLNLVQELNAQMGLTVLMIIHDLNLASEYCDYLLLLNEGK
                     LYKKGTPSEVIDYKTIEEVYNTVVFTQENPLSKKPAVFLVSNKILAESRKNATDKLPD
                     GKS"
BASE COUNT          313 a          217 c          239 g          387 t
ORIGIN      
        1 cgtcgtctcg tcgtgtggtt gaaaacaagc aactggagtt aagaaagatg attcacgacg
       61 aattggtcaa gcgtgtaaaa gaaccctagc tttttccatc gggtaatttg tccgtcgcgt
      121 tttttcggct ttcggccaga attttgtttg aaacaagaaa aactgcaggt tttttcgata
      181 acggattctc ctgagtaaat acaaccgtat tgtacacctc ttcaatggtt ttatagtcaa
      241 taacttcgct tggagtacct tttttgtata gttttccttc attcaagagc aataagtaat
      301 cgcaatattc actggccagg ttcagatcgt gaattatcat aagcacagtg agtcccattt
      361 gggcgtttag ttcctgaaca aggttcaata cctgaatttg atgggagatg tcgagttgag
      421 aggtgggttc atcgaggagc aagagttggg gttcttgtgt caaagccctg gcaatgcctg
      481 caagttgacg ttcacctccg ctcaatttac tcattaattt atctttaaac cggaatacat
      541 cggttaagtg catgtatttt tctgcaattg agaggtcttc tttggtctca aagaattgaa
      601 aatttgtgcg atagggcatg cgtcccatta aaacatagtc ttcaacacta atatcaactt
      661 cctctgtatg ttgggttacg atggcgattt ttgtagcttt ttctctaatc gaaagttggt
      721 tcaggttttt attatcaagc ataatcaggc ctttgctgat ttttaattct ccagtaatgg
      781 ctttcatcag ggtagttttg cccgatccat tagggccaat gactcctgcg aaaacacctt
      841 tgccgatttc gaaattgagc gaatcgagcg aaaaattgcc atagccacat atcaggttct
      901 ttatgctaag ataacttctg ttcacgtgtg ttgagttact aaagtgttgt caataaatcc
      961 aatatagcct gctttatgct gtttccttct attgtttgaa aagaaaatag aacagcagga
     1021 tatgtgttta attatcatta ttttatcatc ttctgttttg agcggcttaa tacaaaaata
     1081 aatacgcttc ctcccaacat cccggtaata acgcctatgg gaagttcgtt tggtgcgata
     1141 atagttcggg caataa
//
LOCUS       DAOPUW010000675         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_145024_length_1156_cov_2.673933, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000675 DAOPUW010000000
VERSION     DAOPUW010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145024_length_1156_cov_2.673933"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PKJ43_09730"
     CDS             complement(<1..677)
                     /locus_tag="PKJ43_09730"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNZ72893.1"
                     /translation="MKRSTVIVTGAARGIGKSVAQYFASKDFNVVVADNNKDEGISTV
                     GHLRDLGYEAIFVKTDVSLLPELENLMRETTGTYGSIDVLINNAGLSEFYDPLEISME
                     KWNRVLSTNLQGVFFASREAVKFMKETGGGSIVNIASTRALMSEPNSEAYAASKGGIL
                     GLTHAMAASFAKYKVTVNAILPGWIETGDYNALKLGDHSQHLSGRVGKPDDIARACFF
                     LSSPENN"
     gene            834..>1156
                     /locus_tag="PKJ43_09735"
     CDS             834..>1156
                     /locus_tag="PKJ43_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNZ72894.1"
                     /translation="MSQERKYVETPLMKQYYAVKDKHPDAVLLFRVGDFYETFGDDAI
                     KASEILGITLTRRANGSASSVELAGFPYHALDTYLPKLVRAGQRVAICEQLEDPKQTK
                     NIVKRG"
BASE COUNT          353 a          266 c          231 g          306 t
ORIGIN      
        1 aagttattct ccggggaaga aagaaaaaag caagcccttg caatatcgtc gggttttcct
       61 acccgccccg ataggtgctg tgaatgatcg cctagcttta atgcgttgta atcaccggtt
      121 tcgatccagc cgggcaaaat tgcattgaca gtaactttat actttgcgaa tgaagccgcc
      181 atagcatggg tcaatcccaa aattccccct ttcgatgctg cataagcctc gctgttcggt
      241 tccgacatca gcgctcgtgt cgaagcaatg ttaacaattg atccacctcc ggtttctttc
      301 ataaatttca ccgcttcacg cgaagcaaaa aatactccct gtaaattggt cgaaagtacc
      361 cggttccatt tttccatgct aatttcaaga gggtcataaa actccgaaag tccggcatta
      421 ttgatcaaga catcgataga gccataggtg ccagtcgttt ccctcatcag gttttccaat
      481 tcgggcaaca aggaaacatc cgttttgaca aaaatcgcct cgtaacccag atcacgcaaa
      541 tgcccaacag tcgatatccc ttcgtccttg ttattatcgg caacaacaac attaaaatcc
      601 tttgacgcaa aatattgagc aaccgacttt ccaatgcccc tggcagctcc agtaactata
      661 accgtactcc ttttcatatg tttttattga aaatgcgctt caaattagca caaaaaaaga
      721 cgctgtggca cttaatgaga aattattgtt gataaaaaca gaaaaagaga ttggcagact
      781 attttctatc gcctgtttaa aaaattatct ttgctgggca cgaagaaata gaaatgagcc
      841 aggaaagaaa atacgttgaa acaccgctta tgaagcaata ttatgcagta aaggataaac
      901 atcctgatgc agtactgctt tttcgtgtag gcgactttta cgagaccttt ggcgatgatg
      961 ctataaaagc atctgaaata ctgggcatta cattaaccag aagggccaat ggatcggcaa
     1021 gttctgttga attggcaggt ttcccttatc atgcactcga tacttacctc cctaaactgg
     1081 taagggcagg gcaacgggtt gccatttgcg aacagcttga agaccccaaa caaaccaaga
     1141 atattgtgaa aagggg
//
LOCUS       DAOPUW010000676         1153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_145648_length_1153_cov_2.478142, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000676 DAOPUW010000000
VERSION     DAOPUW010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145648_length_1153_cov_2.478142"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..542
                     /locus_tag="PKJ43_09740"
     CDS             102..542
                     /locus_tag="PKJ43_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72895.1"
                     /translation="MLYYLPTLEQKLAVGLVYNGSLLFGGGTKAGSFDLDLYGLGLYG
                     VKGQYRFLNRKVSPYVAISTGLSHLETPSFLDENGNLLIPGRNSFSLGLAPEVGVELG
                     FFILSATYFVPMKYKVWSVSKESAGTFQVSFGFRYSLDLKETKI"
     gene            complement(607..>1153)
                     /locus_tag="PKJ43_09745"
     CDS             complement(607..>1153)
                     /locus_tag="PKJ43_09745"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNZ72896.1"
                     /translation="PYDIPGYVKINENNGRNYFNSICYADSCLGVFVNNLRNSQLWDN
                     TMLIVTSDHATPDPGPTMYNDPATYRIPMIWTGGAIDTSFVCNAVAMQTDLSTTLLQQ
                     LGWEAEKTYFSKNIFGNNQYAFFFRDEGWGCLSPEAGFFTDKESGIQQFFYGEKYGKA
                     DSLQRFAKSFTQYLHDDFLKR"
BASE COUNT          297 a          220 c          259 g          377 t
ORIGIN      
        1 acccttgatt ttttgaatta tcgcagaccc tgagtttttc agcagaccct aattacgatg
       61 aaaattcagg agccaaaggt gggcttgggt attcaatcga tttgctttat taccttccca
      121 cacttgagca gaaacttgct gttgggttgg tttacaatgg atcattactt tttgggggtg
      181 gaaccaaagc agggtcgttt gacttagatt tgtatggact tggattgtac ggagttaaag
      241 ggcaatatcg ttttttgaat cgtaaagttt cgccttatgt tgccatttct accggcttat
      301 cgcacctcga aaccccatca tttttagatg agaatggaaa cttgctcatt cccggtagga
      361 actcgttttc acttggactt gctcccgaag ttggcgttga actgggcttt tttattctta
      421 gtgctaccta ttttgtgccc atgaaatata aggtgtggtc ggttagtaaa gaatcggcag
      481 gaacttttca ggtttcattt ggcttccgct attcactcga tctgaaagag actaaaatat
      541 agtgataggt tagtaatttc tgaagttcgg tttttctgct tttatgtgtg ttaacaacac
      601 ctttactcac cgtttcagaa aatcgtcgtg caaatattgg gtgaatgatt tggcaaaacg
      661 ttgcaacgaa tcggctttac cgtatttttc accatagaag aattgttgta ttccgctttc
      721 tttatcggta aaaaagcctg cctcaggcga aagacaaccc catccttcat cgcggaagaa
      781 aaaggcatac tgattgttcc cgaatatatt tttcgagaaa tatgtctttt cggcttccca
      841 ccccaattgc tgtaataatg tagtagaaag atcggtttgc atggctactg cattgcatac
      901 gaatgaggtg tcaatagctc cgccagtcca aatcattggt atacggtatg ttgcaggatc
      961 gttatacatg gttggaccgg gatcaggtgt ggcatggtct gaggtgacaa taagcatagt
     1021 gttgtcccat aattggctat tccttaaatt gttgacaaaa acaccaaggc aactgtcggc
     1081 atagcatata gagttgaaat agttgcggcc gttattttcg tttatcttca catagcctgg
     1141 gatatcgtat ggg
//
LOCUS       DAOPUW010000677         1152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_145784_length_1152_cov_3.257065, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000677 DAOPUW010000000
VERSION     DAOPUW010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145784_length_1152_cov_3.257065"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1152
                     /locus_tag="PKJ43_09750"
     CDS             <1..>1152
                     /locus_tag="PKJ43_09750"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HNZ72897.1"
                     /translation="AVVIIGGDDSNTNACVLAEYYASINAGVQVIGCPKTIDGDLKNE
                     MIETSFGFDTACKVYSELIGNIMRDANSAKKYWHFIKLMGRSASHIALECALQTHPNI
                     CLISEEVASKKQTLSEVVDYIADIIAKRGDEGLNFGIALIPEGLIEFIPEMNTLISEL
                     NDLLAEGTATAQAFQHVKKSHRVDWTAEKLSSKSSVVFRSLPEGIANQLTLDRDPHGN
                     VQVSKIETEKLLIEMVEAELAERKLEGKYKGKFGAQNHFFGYEGRCAAPSNFDADYCY
                     SLGFNAAVLIANGKTGYMSSVRNTTAPASEWIAGGVPITMMMNMERRHGHMKPVIQKA
                     LVELDGKPFSFFASQREKWAVETDFVYPGPIQYFGPSDVCDRVSETLKLE"
BASE COUNT          331 a          226 c          256 g          339 t
ORIGIN      
        1 gcagtagtta ttattggtgg tgacgattcg aataccaatg catgcgtgtt ggccgaatat
       61 tacgcttcaa taaatgctgg cgttcaggtt atcggttgtc ctaaaaccat cgatggcgac
      121 ctcaaaaatg aaatgatcga aacatctttc gggttcgata ctgcttgtaa agtgtattcc
      181 gaattgatag gaaacattat gcgtgatgcg aattctgcaa aaaaatattg gcactttatt
      241 aaacttatgg ggcgttcagc ttcacacatt gcacttgaat gtgcccttca aactcatcca
      301 aacatttgtt taatctcgga agaagtggct tcaaaaaagc aaaccttgag cgaagttgtt
      361 gactacattg ccgacattat tgcaaaacgt ggcgatgaag gcttaaattt tggtattgca
      421 ctcattcctg aagggttgat cgaattcatt cctgaaatga atacccttat ttctgaattg
      481 aacgatttgc ttgccgaagg aactgctaca gcacaagcat ttcagcatgt taaaaaatca
      541 catagggttg attggactgc cgaaaaactt tcatcaaagt cgtcagtagt attccgttcg
      601 ttgcccgaag gtattgctaa tcagcttacc ctcgaccgcg atccccatgg aaacgttcag
      661 gtttctaaaa ttgaaactga aaagctgctg attgaaatgg ttgaagcaga actggccgag
      721 cgtaaactcg aaggaaaata caaaggtaaa tttggtgctc aaaatcactt ctttggatat
      781 gaaggccgtt gtgctgcccc ttctaatttc gatgctgact attgctactc tttaggtttt
      841 aatgcagcag tgctcattgc caacggtaaa actggttata tgtcatcggt taggaatact
      901 acagctcctg caagtgaatg gattgccggt ggagttccaa tcactatgat gatgaacatg
      961 gaacgtcgcc atggtcacat gaaaccggtt attcagaaag ctttggttga actggatgga
     1021 aaaccgttta gtttcttcgc ttcacaacgt gagaaatggg ccgttgagac cgatttcgtt
     1081 tatcctggtc ctatccagta ttttggtcct agcgatgtat gtgaccgcgt ttcagaaaca
     1141 ttgaaactgg aa
//
LOCUS       DAOPUW010000678         1152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_145823_length_1152_cov_2.542388, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000678 DAOPUW010000000
VERSION     DAOPUW010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145823_length_1152_cov_2.542388"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..618)
                     /locus_tag="PKJ43_09755"
                     /pseudo
     CDS             complement(1..618)
                     /locus_tag="PKJ43_09755"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            877..>1152
                     /locus_tag="PKJ43_09760"
     CDS             877..>1152
                     /locus_tag="PKJ43_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNZ72898.1"
                     /translation="MNTFRFFGWGTLFLVVVLISGCSPKQGKEKAGGKGRQVPKVDAY
                     VVSPSRVVNELTVSGSLLPFEEVVLKNEVAGRVVKLNLPEGKFVKSGT"
BASE COUNT          317 a          224 c          246 g          365 t
ORIGIN      
        1 cagtagttct tcttgctgaa cattggttgt gctctcgaac ccattataga atgttttccc
       61 ctcgagcccg gctacacgta tgctggcaat tcctgaaaca ttgaaatatg aatgaagtgc
      121 agaggaataa gaaagtggtt cagagccggt gtttgtaact gttagtgaca catcaaggct
      181 gcggccaatg attacagtca gcaatgcccg aaaatcgtgt ggccaaaggg ctttggtttc
      241 ctcatcagaa gtaagtcgca gtataacttt tgtatcgccg gtttcgagcg acgcagtttc
      301 aacaacttgc tagtatttta gccgtgcaaa cccatgcata ggtttacctg ggtcggtcgg
      361 atgtgggccg aaccatggaa aacaaaccgg gattccgccc ctgatggctt ccccttcttc
      421 aaaaaagcta gactcgctta cccaaagaac atcgaaagag ccttgtggtg tgtagcgcat
      481 tacctggcca ccgtaaaggc ttatttcggc atcagcatat ttgttaaaga ttgaaatggc
      541 cagaaggtca ccttccgatt cgataaaacc caactctcct tcaatgccaa atttttcatc
      601 taattcgtct gttgtcatca tatcttttta ctgctaaagg tagttttttg tgtaaatctg
      661 gcaaaggaga gtttttttta tccttacttt tggagatatt tttttaaatc gagttaacat
      721 cttttttatc aaataaattg acatttaaac ggttaataat gacaatattt gaattattgc
      781 cgattttaac ctcttattaa caacacaatt tattattcaa ataaaccttt atcagtattt
      841 ttggtcttat ttttttaata gtaaatacat atttcaatga atacatttcg atttttcggc
      901 tggggaacac tgttcctcgt tgttgtgctt atttcaggtt gcagtcctaa gcagggcaaa
      961 gagaaggctg gcggaaaagg ccggcaagtt cccaaagtcg acgcttacgt ggtttctcct
     1021 tcgcgggttg tgaatgagtt aacggtttca gggtcattac tgccttttga agaagttgtg
     1081 ttaaaaaacg aggttgcagg cagggtagta aagttgaatt tgcccgaagg aaaatttgtg
     1141 aaatctggca ca
//
LOCUS       DAOPUW010000679         1141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_148021_length_1141_cov_3.053407, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000679 DAOPUW010000000
VERSION     DAOPUW010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148021_length_1141_cov_3.053407"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..955)
                     /locus_tag="PKJ43_09765"
     CDS             complement(14..955)
                     /locus_tag="PKJ43_09765"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72899.1"
                     /translation="MNIGLDVMGGDYAPDAVIAGAVLAQKHLSNSERITLFGDENAIL
                     RLLKNENANVSDFDIVHCSEVIAMGDNPAKTFSKKIDSSIHKGFKHLAEKKIDGFASA
                     GNTGAMLVGTMYTIRSVPGVIRPAIASYIPVSEKKFNLILDVGINPDCRPDVLFQYAI
                     LGSLYSKHVFGIDKPRIGLLNIGAEDEKGNLVTKSTFELMKDTSDFHFAGNVEGNDLF
                     SDSKADVFVCDGFVGNVVLKEAEAFYQLIKKRNITDHFFEQFNFENYGGTPILGVNSP
                     VVIGHGISNAKAIMNMILQTRTVISSNLCEKIKEAFN"
     gene            complement(1004..>1141)
                     /gene="rpmF"
                     /locus_tag="PKJ43_09770"
     CDS             complement(1004..>1141)
                     /gene="rpmF"
                     /locus_tag="PKJ43_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNZ72900.1"
                     /translation="RTHYKAEEPTLAKCSNCGATYQLHRVCPECGFYRGKLAVEKTIT
                     A"
BASE COUNT          327 a          245 c          230 g          339 t
ORIGIN      
        1 gtaatctttc ccattagtta aaggcttctt ttattttttc acaaaggttt gaacttatca
       61 ccgttcgggt ttgtagaatc atattcatta tcgccttggc gttggaaatc ccatggccaa
      121 tgacaacggg ggagtttact cccagaatag gagtaccgcc ataattctca aagttgaatt
      181 gctcaaaaaa atgatcggta atattcctct ttttaatcag ctgataaaag gcttcagctt
      241 ctttaagaac aacattccca acaaatccat cgcaaacaaa cacatcggct ttcgaatcgg
      301 aaaacaggtc gttgccttca acattcccgg caaaatgaaa gtcagaagta tccttcatta
      361 gttcaaaggt acttttggta accaggttac ctttttcatc ttcggcgccg atattcagta
      421 ggccaatcct gggcttgtcg ataccaaaaa cgtgttttga atacaatgaa ccaaggatcg
      481 cgtactggaa aagtacatca ggacgacaat cggggttaat gccaacatcg agaataagat
      541 taaatttttt ctccgataca ggtatatagc tagcaatggc aggccgaatt acaccgggta
      601 ctgacctaat ggtgtacatt gttcctacca gcattgcacc tgtgtttccg gcactggcga
      661 agccatcaat ttttttctcg gcaaggtgtt taaagccctt atgtatgctc gaatcgattt
      721 ttttgctgaa tgtttttgcg ggattgtctc ccatggcgat tacttcggaa caatgaacaa
      781 tgtcaaaatc ggaaacattg gcattctcgt ttttaagtaa tcgcaaaatg gcgttttcgt
      841 ccccgaaaag tgttatcctt tcggagttgc taagatgttt ttgagctaaa acggctccgg
      901 caataacagc atccggagca tagtctccgc ccataacatc aaggccaata ttcatatgca
      961 aaaacaaata ataggattgt tatcaagcct gctatacagg ttactatgct gtaatcgttt
     1021 tttcaacagc aagttttcca cgataaaaac cacattcggg gcaaacacga tgaagctggt
     1081 aagtagctcc gcagttggag cacttggcca gggttggctc ttctgcttta tagtgtgttc
     1141 t
//
LOCUS       DAOPUW010000680         1135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_149441_length_1135_cov_2.281481, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000680 DAOPUW010000000
VERSION     DAOPUW010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149441_length_1135_cov_2.281481"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..>1135)
                     /locus_tag="PKJ43_09775"
     CDS             complement(230..>1135)
                     /locus_tag="PKJ43_09775"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNZ72901.1"
                     /translation="QQTIFSGLVDYATYGPKSPFTNIVSNKELKALKGNVLTDKIKEL
                     FGYKHKVLYYGTYSAEDLKQMLAKVHAVPENFKAAPEKSPFTELETGKNRVLFSHYEA
                     NQSYLQMISKSVDYDFNLLPNARMYNAYFGGGMNTIVFQELREKRGLAYTARSFFATP
                     SNPDGPFMNNSFIATQNDKVIEAFSAFNELFDEMPMSEISFNLAKDAIISGIRNQRIT
                     KMNLIMNYINAQKMGYKKDIRQTYFETIPNMTLEDVKQFNEKYIKGKPKTYVILGNEK
                     IVDFKQVEKQFGPVTKLTREQIFTY"
BASE COUNT          304 a          230 c          243 g          358 t
ORIGIN      
        1 catggaaagt aaaaataaat gtggtagcca tctttggcgg ctatgccgac aaaagaaaaa
       61 agctcattac agataacggc aatgccaatg gcagcgagtt aataataagc gggattgtga
      121 tttttggcgg tggcgatatt aaaagctact aacattcacc tataacaaac taaagagccg
      181 ccccgttttt cgaaggggcg gctctttgtt atatcaacag gggtatcgat taataggtaa
      241 atatctgttc gcgcgtcaac ttagttacag gaccaaactg tttttcaacc tgtttgaaat
      301 ccacaatctt ctcattccca agaattacat atgttttagg tttgcctttg atgtactttt
      361 cgttgaactg cttaacatcc tccagggtca tattggggat tgtttcgaag taggtttgac
      421 gaatatcctt tttgtatccc attttttgcg cattgatgta gttcataatc aaattcattt
      481 ttgtaatacg ctgattacgt atcccgctga ttatggcatc cttggccagg ttgaaggata
      541 tttcagacat gggcatctcg tcaaacagct cgttgaatgc tgagaaagcc tcaatcacct
      601 tgtcgttctg ggtggcaata aacgagttat tcatgaatgg gccatcggga tttgacgggg
      661 tggcaaagaa ggagcgtgct gtgtatgcca atcctctttt ctctcttaac tcctgaaaga
      721 caatggtatt catacctcca ccgaagtaag cattgtacat ccgggcattg ggaagaaggt
      781 taaaatcgta gtcaacactt tttgaaatca tctgtaaata cgactgattt gcctcgtaat
      841 gcgaaaacag caccctgttc ttaccggttt ccaattcggt aaacgggctc ttttcgggag
      901 cggctttaaa gttttcggga acggcatgta cttttgcaag catttgcttt aaatcttcgg
      961 ctgagtatgt tccgtagtac aacaccttgt gtttgtatcc aaacagctcc ttgatcttgt
     1021 ctgtcaacac atttcctttc aatgctttca actctttgtt cgatactata ttggtgaatg
     1081 gcgatttggg cccataagta gcgtaatcaa ccaatccgct gaaaatggtc tgttg
//
LOCUS       DAOPUW010000681         1131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_150322_length_1131_cov_1.952602, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000681 DAOPUW010000000
VERSION     DAOPUW010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150322_length_1131_cov_1.952602"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1131
                     /locus_tag="PKJ43_09780"
     CDS             <1..>1131
                     /locus_tag="PKJ43_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNZ72902.1"
                     /translation="INYYFGDINAKANYTFSLTDRIYLNIYLGKDKAKMSEKNKTDVK
                     QNVNSQSISWGNSIASLRWSHLFRKNSFMNTTLYFSGYDYKTQIKELFTSDEYSENFY
                     YNYKSGIQDVGVRSDVEVPFMGNYYFKSGVSLVGHFFDSGSKTFQGSLVNTANDYFVP
                     DSLVKNSFASESSLYFELGGSILHRLQFLFGVHSNLFLYENSKFSTIEPRAMVSYSLR
                     RNFSVNASYTQMGQNIHLLTNATIGMPTDYWLPAQSHLLPEKASQYSVGASFNNSSYR
                     ISIDLYTKQMKNLIAYLDGVSFMENPVDNIDKIVSGNGSSKGIEFFVEKKKGKLTGWL
                     SYTLSKTDREFASINAGKPFPFDYDRRHNFAVAGVYQLKKNV"
BASE COUNT          321 a          209 c          235 g          366 t
ORIGIN      
        1 gtatcaacta ttattttggc gatataaatg ctaaggcaaa ttacacgttc agtttaaccg
       61 accggattta cttgaacata tatttgggaa aagataaagc aaaaatgtcg gagaaaaata
      121 agactgatgt taagcagaat gttaatagcc agtctatttc ctggggaaat tcaatagctt
      181 ctttacggtg gagccacctt tttcggaaga attcttttat gaatactacc ctctattttt
      241 cgggctatga ttacaaaacc cagattaaag aacttttcac ttctgatgag tattccgaaa
      301 acttctatta taactacaag tctggcatac aagatgttgg tgttcggtct gatgttgaag
      361 ttccttttat ggggaattat tattttaaat caggggtgtc tttggttggg cattttttcg
      421 attcagggag caaaaccttt cagggaagcc ttgtaaatac agcgaacgat tattttgtcc
      481 ccgattctct ggtgaaaaat tcatttgcgt ctgagtcttc attgtatttc gagcttgggg
      541 gtagcatttt acaccggctc caatttcttt ttggtgttca ttcgaacctt tttttgtatg
      601 aaaacagtaa attctcgacc attgagccaa gagcaatggt atcatattcg ctgcgtagga
      661 acttttcggt caatgcttcg tatacacaga tgggacaaaa tattcacttg ctgaccaatg
      721 caactatcgg gatgcctacc gactactggt tgcctgcaca aagccacctt ttgcccgaaa
      781 aagcttcaca atattctgtt ggtgcgagct ttaacaattc gtcctatcgt atttctatcg
      841 atttgtatac caagcagatg aaaaacctca ttgcctatct cgatggggtt tcttttatgg
      901 aaaatcctgt tgacaatatc gataaaattg tgtcgggaaa cggctcaagc aaggggattg
      961 aattttttgt tgagaaaaag aaaggtaaac tcactggctg gcttagttac acgctttcga
     1021 aaaccgatag ggagtttgca tcgataaatg ctggtaagcc atttcccttt gattacgacc
     1081 ggcggcacaa ttttgccgtt gccggtgttt atcaattgaa aaagaatgtg c
//
LOCUS       DAOPUW010000682         1130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_150582_length_1130_cov_1.683721, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000682 DAOPUW010000000
VERSION     DAOPUW010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150582_length_1130_cov_1.683721"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PKJ43_09785"
     CDS             complement(<1..861)
                     /locus_tag="PKJ43_09785"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNZ72903.1"
                     /translation="MKVKLKIRQKILIYILSLFTVLYLFSLGYIVYKSRETIIKETKQ
                     NSVLVAQNAAGNIRHFFEKNLTITRTLSKAFTVYRELPPKQWQDLFLKMYMPVIKANP
                     NVYIVWDSWEYYGFVPGYTKGYGRILQSVVREGSKFTISVEERSMDGDPERYGNFKQN
                     NVDAIWEPYPDQVQEDIREPRLMTTIASPIQIEGKFMGLIGLDVELTYLQELVKSIKL
                     PEGGYAFLLSSDGVIAGHPDGSLIFKNVSEILPDEAGLHNILQRVQKGENFEFSRYNG
                     KDEHLMLFTPL"
     gene            complement(959..>1130)
                     /locus_tag="PKJ43_09790"
     CDS             complement(959..>1130)
                     /locus_tag="PKJ43_09790"
                     /inference="COORDINATES: protein motif:HMM:NF024928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HNZ72904.1"
                     /translation="VTCHKAQGGQWKEVYIDQGFFKEDMISKEYLRWLYTALTRATDK
                     VYFVNFNPLFFE"
BASE COUNT          328 a          244 c          214 g          344 t
ORIGIN      
        1 gagtggagtg aacagcatca ggtgttcatc cttcccgtta taacgactga actcgaaatt
       61 ttcgcccttt tgaacacgct gtagaatgtt gtgaaggcct gcctcgtcag gcaggatttc
      121 agaaacgttt ttaaaaatca acgacccatc gggatggcca gcaataaccc catcggacga
      181 tagcaaaaag gcatagcctc cttcgggcag tttaattgat ttaacaagtt cttgcaaata
      241 ggttaattca acatcaaggc caatcaaccc catgaacttc ccttcgattt gtattggcga
      301 agcaatagtt gtcatcaaac ggggttctct aatatcttcc tgtacttgat cagggtatgg
      361 ctcccaaatt gcatcaacat tattctgttt aaaatttcca tagcgctccg gatcgccatc
      421 catacttctt tcctcaaccg atatggtgaa tttactacct tcgcgaacaa ctgattgcaa
      481 aattcgaccg taaccctttg tgtatccggg gacaaagcca tagtattccc aactatccca
      541 tacgatgtaa acgtttggat ttgccttaat aacaggcatg tacattttta gaaaaagatc
      601 ctgccattgt ttgggaggga gttcacgata aaccgtaaac gcctttgaca gtgttcgggt
      661 aatggttaaa tttttctcaa aaaaatgccg aatattccca gcagcgttct gcgccaccaa
      721 tacagaattc tgtttagttt ctttaataat ggtttcgcgc gatttataaa caatatatcc
      781 cagcgagaac aggtacaata cggtgaatag ggataatatg taaataagta ttttctgacg
      841 gatcttcagc tttactttca tgatgaggta ggcaatattt ttggctaaat taactatttt
      901 ttacgtgctc tggtttgttc aacgggcact tttttggagc attttatgaa atggataatt
      961 actcaaagaa caacgggtta aaatttacga aataaacctt gtccgttgca cgggttaatg
     1021 cggtgtaaag ccatcgcaaa tactctttgc ttatcatatc ctctttaaaa aaaccctgat
     1081 cgatataaac ttccttccat tgaccgccct gtgccttatg gcacgtaacc
//
LOCUS       DAOPUW010000683         1125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_151482_length_1125_cov_4.133645, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000683 DAOPUW010000000
VERSION     DAOPUW010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151482_length_1125_cov_4.133645"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PKJ43_09795"
     CDS             <1..380
                     /locus_tag="PKJ43_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNZ72905.1"
                     /translation="WFDYYNKESFTVSNASLDLSLAPGEFKIFSTREFLHANISAVGS
                     QSVVKPTICVFPNPANSQLFILGEQLEIQGVYSVLGEKTDVLVYWQTDGVNLDISSLR
                     KGIYIVRGIVRGGQYFTSKFIKE"
     gene            complement(428..>1125)
                     /locus_tag="PKJ43_09800"
     CDS             complement(428..>1125)
                     /locus_tag="PKJ43_09800"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNZ72906.1"
                     /translation="LSALKDGPFITVSNHPYGGIDGIMLVDLMAEIRPDYKLMANQIL
                     SLVKSMEENFISVKPKVGNQSLKPTVNINGIRETITHLRNGHPIGFFPAGAVSMFKFK
                     HMQVRDREWQESILKLIHSAKVPILPIRFFDKNSPLFYFLELIDWRIRLIQMPRELFN
                     KKGKRTRLGIGNIISVEEQAQFDTPHSLGKHLQNLIYTMPLPSTFQRKADIPILQEIK
                     KNNIVIASSDTTI"
BASE COUNT          309 a          197 c          256 g          363 t
ORIGIN      
        1 gatggttcga ttattataat aaagaatcat ttacagtgag taatgcttcg cttgacttga
       61 gcctggctcc gggagagttt aaaattttct caacgcgaga gttcctacat gcaaatattt
      121 cggcggttgg gagtcagtct gttgttaagc ctaccatttg tgtctttcca aaccctgcaa
      181 acagtcagct tttcatacta ggagagcaat tggaaataca aggggtttat tcagttctgg
      241 gcgaaaagac cgatgtgctt gtttattggc aaactgacgg agttaacttg gatatttctt
      301 cacttcggaa aggaatatac atagtgagag gcatcgttag gggtgggcaa tattttactt
      361 cgaaatttat aaaagaataa caaatacagg cagaagtttt cccattcttt tggattttct
      421 atagttatta aatagtagtg tcgctactgg ctattacgat attattcttt ttaatttctt
      481 gtaatatagg aatgtcggcc ttgcgttgaa aggttgatgg tagcggcatc gtataaatta
      541 gattttgcaa atgtttaccc aacgaatgag gtgtatcaaa ctgggcttgc tcttcaaccg
      601 aaatgatgtt tccaatgcca agccgggttc tttttccttt tttgttgaat aattcgcgag
      661 gcatctgaat taagcgaatg cgccagtcga tcagttcgag gaaatagaaa agtggtgaat
      721 ttttatcgaa aaagcggata ggcaaaattg gtacttttgc agaatgaatc agtttgagga
      781 tactttcctg ccattctcga tcgcgtacct gcatgtgttt gaacttgaac atagaaacag
      841 ctcctgcagg gaaaaaacca attgggtggc cattccgcaa atgggtaatg gtttccctga
      901 ttccgttgat atttacagta ggttttaacg attggttccc aactttgggt ttaaccgaga
      961 tgaaattttc ttccatcgac tttacgagtg aaagaatctg attggccatc aacttataat
     1021 cgggtctgat ttctgccatt aagtcaacta gcataatgcc gtcgattcct ccgtaggggt
     1081 gattggatac tgttatgaac ggtccgtctt tcagggcact gaggc
//
LOCUS       DAOPUW010000684         1118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_153092_length_1118_cov_2.700847, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000684 DAOPUW010000000
VERSION     DAOPUW010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153092_length_1118_cov_2.700847"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1017
                     /gene="hisG"
                     /locus_tag="PKJ43_09805"
     CDS             157..1017
                     /gene="hisG"
                     /locus_tag="PKJ43_09805"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNZ72907.1"
                     /translation="MSEILRIAIQTKGRLNEESEELIRECGIKLTVGKRQLLSPAKNF
                     PVEILYLRDDDIPETVADGVADVGIVGENEMLEKDKNVVLSKRLGFSKCRLSLAIPKG
                     ESYNGPEWFEGKTVATSYPVILQNYFDQKGIKAAIHVITGSVEIAPGIGLADGIFDIV
                     SSGGTLVSNGLKEVEVVTPSEAVLISTLGLSPEKQAILNELIFRIESVERSKGKKYIM
                     LNAPNGKLPEIVEMLPGMKSPTIMPLAESGWSSLHSVIEEKFFWENISRLKALGAEGI
                     LVLPIEKMIM"
BASE COUNT          363 a          221 c          244 g          290 t
ORIGIN      
        1 atgataacat gttttggaca tcatcacggc caccatcaca acctgcaaaa gcggtaggct
       61 ggcacgatta tgtctgaact ctataaagat atttagataa cgaacgaagg cttacccaat
      121 tggtaagcct tttttatttt tgtaaaaact cgaattatga gcgaaatttt aagaatagca
      181 attcaaacca aaggccggtt aaacgaagaa tcggaagaac tcatcagaga atgtggtata
      241 aaacttaccg tgggaaaacg ccagttatta tcgcctgcca aaaatttccc tgtggaaatc
      301 ctttacctgc gcgacgacga tatccctgaa actgtagccg atggggttgc cgacgttgga
      361 attgttggcg aaaacgagat gctcgagaaa gataaaaatg ttgttttatc caaacgtctg
      421 ggtttcagca aatgtcgtct atcgttagcc attccaaagg gtgaatcata caacggtccc
      481 gaatggttcg aaggaaaaac agtagcaaca tcgtatcctg ttatccttca gaattatttc
      541 gaccaaaaag gcatcaaagc tgcaattcat gtaattactg gttcggtgga aattgccccg
      601 ggaattggac ttgccgatgg aattttcgac atagtaagct ctggcggaac actggtttcc
      661 aatggattaa aagaagttga agtagtgacc ccttccgaag cggtactaat atcaacgctt
      721 ggattatcac ctgaaaaaca agcaattctt aatgaactta tctttcgcat cgaatcagtt
      781 gaacgttcaa aggggaaaaa atacatcatg ctgaatgccc ccaacggcaa gcttcccgaa
      841 attgtagaga tgcttcccgg gatgaaaagt ccaaccatta tgccattggc cgaaagtggc
      901 tggagttctc tgcattcggt tattgaagaa aagtttttct gggaaaatat ctcaagattg
      961 aaagcccttg gtgccgaagg tattttggtg ttgcccattg aaaagatgat catgtaatga
     1021 atcaaatgaa acacgaaggc gcaaagacac ggagaaatga tcccttaagt agaattaaaa
     1081 gacacctaaa aagcattgcg ccctttgtga tgaacctt
//
LOCUS       DAOPUW010000685         1118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_153120_length_1118_cov_2.413923, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000685 DAOPUW010000000
VERSION     DAOPUW010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153120_length_1118_cov_2.413923"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PKJ43_09810"
     CDS             complement(<1..911)
                     /locus_tag="PKJ43_09810"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ72908.1"
                     /translation="MEKNIVVGIIGAGMIAEKHIVGLQKTGRAKVKWVARENGEKLKS
                     FQEKYSIPFGTTDYRDILADSEVDAVVITAPPAFHYAMFVDAVNAGKHILLEKPAAVS
                     KSHVDSMVALAAEHPELLISDCSSRHARLQPKFKIVKSIIDSGKLGEIYFIHHNAVAR
                     QSRPGIEYHPSAKWFLNKNIAGGGVLFDWGVYDLSFHLGVLGDVPQLEKSDLLFMKNG
                     LDNVDPGTDIFDVEEHFAVSMQFSGGLRYYWEHATNANMEATNETRIYGTRGGIKLSF
                     CSWENPDIEFFDVEDQGKGKARKELIHV"
BASE COUNT          335 a          254 c          211 g          318 t
ORIGIN      
        1 acatgaatca actccttgcg ggctttacct ttcccttgat cttcaacatc aaagaactcg
       61 atatcagggt tttcccacga gcaaaatgaa agtttaattc ccccacgggt accatagata
      121 cgggtttcgt tggttgcttc catgttggca ttggttgcgt gttcccagta atagcgcaag
      181 ccaccggaga attgcatcga cacagcaaaa tgttcttcaa catcgaaaat atcggtaccc
      241 ggatcaacgt tatcgaggcc atttttcata aacagcaaat ctgacttttc caactgaggt
      301 acatcgccaa gcacccctaa atggaacgac aagtcgtaga ctccccaatc gaaaagaacg
      361 ccaccgcctg ctatgttttt atttaagaac catttagccg aaggatgata ttcaataccc
      421 ggtctcgact gacgagcaac tgcattatgg tggataaaat agatttcccc aagctttcca
      481 gagtcgataa tagacttcac aattttaaat ttcggctgta gtcgggcatg acgcgatgaa
      541 caatcgctga ttaacaattc agggtgctcg gccgcaagtg ctaccatcga atcaacatga
      601 gacttgctca ctgctgcagg tttttcgagc aaaatatgct tacctgcgtt caccgcatca
      661 acaaacatgg cataatgaaa agcaggaggc gcggtaatta ctacagcatc aacctcagag
      721 tcggcaagga tatccctata atcggttgtt ccgaagggga ttgagtattt ctcctgaaac
      781 gatttcagtt tttcaccatt ttcgcgggct acccatttaa cctttgccct gccagttttt
      841 tgaagaccaa caatatgttt ctcggcaatc atgcctgcac caataattcc aactactatg
      901 tttttttcca ttgatagtaa aaaaattata agattacaaa gtgaaccaat aaaagcagtg
      961 tttataaatt caataagtac cgctcagaaa aatcacttgt ttttcgtgaa cgaaattatt
     1021 cgtttatttc gggtagaaat gtttaagaca tgaaaaatac gctttttttt acaatctttt
     1081 tggtgatcac tgtaataacc acaatggctt ccgggcaa
//
LOCUS       DAOPUW010000686         1117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_153294_length_1117_cov_2.834275, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000686 DAOPUW010000000
VERSION     DAOPUW010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153294_length_1117_cov_2.834275"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="PKJ43_09815"
     CDS             complement(<1..1098)
                     /locus_tag="PKJ43_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72909.1"
                     /translation="MRKPLKITLITVVALFVLCGAMVGYLLWFVATPPKLTSALKQQV
                     PKFINCHYEIGEVEITFFRTFPKFGIRANDLILINPVPGAPTDTLAAIDEVVGTVDIS
                     AWFKNNELVVDELICKNSAVTIFYDSLGHSNFDVVVYSSEDTVATTFIAGLNNIQFEN
                     VDFRYVDNSLKTKVLIDDLYGTLSGSFVSGTLISDISIDNSIITVGYSGENYLDRAAT
                     SLKLVSEISVSPMSVNIKEASGSIASLPVFAEGLLKEDTVTGDLLFDVDYQIKESQAD
                     QILSMVPTSYSSYFDGITLEGNVAVDGTIKGTYNQSTMPMFGVSLRLNDAKAAYRGIP
                     LQLHDLNGQLEIATDGMTDSLTYVTIKNLTGK"
BASE COUNT          357 a          244 c          212 g          304 t
ORIGIN      
        1 tttcccggta aggttcttta tcgtaacata cgttaacgaa tcggtcatac catcggtggc
       61 aatttcaagc tgtccgttca gatcgtgcaa ctgcaaagga attccacgat aagccgcttt
      121 tgcatcatta agcctcagac tgactccgaa cataggcatc gtcgattggt tgtaagtacc
      181 cttaattgta ccatcgacag caacattgcc ctcaagcgta atcccatcga aataagaaga
      241 atatgaagta ggaaccatcg acaatatctg atctgcctgt gattctttta tctgataatc
      301 aacatcgaaa agcaggtcac ctgtaaccgt atcttctttc aacagcccct cggcaaacac
      361 tggcaacgaa gcgatggaac cgctagcttc tttaatattg acagacatag gtgatacaga
      421 aatttcggac acgagtttaa ggcttgttgc ggccctatcg aggtagtttt cacctgaata
      481 tccaacagta atgatgctat tgtctataga aatatcacta ataagagttc ccgaaacaaa
      541 cgacccactg agcgttccgt acaaatcatc gatgagcact ttggttttca gtgaattgtc
      601 aacatatcga aaatcaacat tttcgaattg aatattgtta aggcctgcaa taaatgtggt
      661 tgctactgta tcttcggaag aataaaccac aacatcgaag tttgaatgtc caagactgtc
      721 gtagaaaatt gtcaccgcag aatttttaca aatcaattcg tcaacaacaa gttcgttgtt
      781 tttaaaccaa gctgaaatat caacagtacc cactacttca tcaatggcag ccagcgtatc
      841 ggttggagca cccggaaccg gattaattag gataagatcg ttcgccctaa taccaaactt
      901 tgggaaagtg cgaaaaaaag taatttcaac ttcgccaatt tcgtaatgac aattgataaa
      961 cttgggaacc tgctgcttaa gtgccgaggt gagttttgga ggagttgcca caaaccaaag
     1021 caaataacct accattgcac cacaaagaac aaacaatgca acaacagtta tcagtgttat
     1081 tttcagcggt ttgcgcatgc ttaattatat ttgtcaa
//
LOCUS       DAOPUW010000687         1115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_153802_length_1115_cov_2.045283, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000687 DAOPUW010000000
VERSION     DAOPUW010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153802_length_1115_cov_2.045283"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PKJ43_09820"
     CDS             <1..779
                     /locus_tag="PKJ43_09820"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNZ72910.1"
                     /translation="FDDFETNAVGTLNLLEAARQFCGDSPFIYFSTNKVYGDSPNKIR
                     LKELASRWEFDDPAYENGIDETMSIDQTTHSLFGASKVAGDVLVQEYGRYFGLPTCCL
                     RGGCLTGPSHSGVELHGFLSYLIKCNLEEREYNIFGYKGKQVRDNIHSADVASFVYAF
                     FKKRRFGEVYNLGGGHNNSCSIIEAFSMVAEVTGKPQRFSYQDKNRIGDHICYYSDLR
                     KIRLHYPEWDIKHSLRSIVEQTCLSWIERMHKDANAKAAC"
     gene            823..>1115
                     /locus_tag="PKJ43_09825"
     CDS             823..>1115
                     /locus_tag="PKJ43_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNZ72911.1"
                     /translation="MKLLITGICGFVGSSLAKFWHNRADGTEVFGIDNFSRRGSEINR
                     EILTKMGIKVWHGDIRVNCDLEALPRADWVIDAAANPSVLAGVSGNMVSRQLV"
BASE COUNT          307 a          208 c          275 g          325 t
ORIGIN      
        1 catttgatga ttttgaaaca aacgccgtag gaactctgaa tttacttgag gcagccagac
       61 aattttgtgg tgactcgccc tttatatatt tttcaacaaa caaggtttac ggtgattccc
      121 ctaacaaaat cagattgaaa gaactggctt cacgttggga gtttgacgac cctgcttatg
      181 aaaatgggat tgatgaaacc atgtcgatag accagacgac acattcgtta tttggcgctt
      241 ctaaggttgc tggcgatgtt ctggtacagg aatacgggcg atattttggg cttccaacct
      301 gttgccttcg gggaggttgc cttaccggcc cttcgcattc cggagtcgaa ctacatggct
      361 ttttaagcta tcttataaaa tgcaatcttg aggaacgtga atacaatatt tttggctaca
      421 aaggcaagca ggttcgcgat aacattcact ccgccgatgt tgcaagtttt gtctatgcat
      481 tttttaaaaa acgaagattt ggcgaggttt ataacctggg tggtggacac aacaactcat
      541 gctcaattat tgaagctttt tcaatggttg ccgaggtaac tggcaagcct cagaggtttt
      601 cttatcagga caaaaatcgt ataggggatc atatctgcta ttacagcgac ctgagaaaga
      661 tcagacttca ttacccggaa tgggatatta aacactcact gcgttctatt gttgaacaaa
      721 cctgcctgtc ctggattgaa agaatgcata aggacgccaa tgcaaaggct gcgtgttgaa
      781 atttttgaaa atttgcgcaa tagcgattaa gggttggatc gggtgaaatt acttattact
      841 ggcatttgcg gatttgtggg ttcgagcctt gcaaaatttt ggcataaccg ggcagatggc
      901 accgaagttt tcggcataga taatttttcc cgtagaggta gtgaaatcaa tcgggaaata
      961 ttaacaaaaa tgggaataaa agtctggcac ggagacataa gggttaattg tgatttagag
     1021 gcgctgcccc gtgctgactg ggtaattgat gctgctgcta acccaagtgt tctggctggg
     1081 gtgtctggta acatggttag tcgccagttg gttga
//
LOCUS       DAOPUW010000688         1112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_154412_length_1112_cov_2.421003, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000688 DAOPUW010000000
VERSION     DAOPUW010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154412_length_1112_cov_2.421003"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            344..898
                     /locus_tag="PKJ43_09830"
     CDS             344..898
                     /locus_tag="PKJ43_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNZ72912.1"
                     /translation="MEEQIRQIAERIRGLREVLDISVEDAATTCGISIEKYLEYESGK
                     VDIPVSVLHNISKKYNVELTVLLTGEDPHMHQYSLTRKNTGAAVERTKAYKYQSLAQG
                     FINKKAEPFIVTVEPKPEGCCQSLNQHPGQEFNYILEGKLKFCLDGKEMILEEGDSIY
                     FNSGLRHGMLALDEKPCRFLAIIF"
     gene            917..>1112
                     /locus_tag="PKJ43_09835"
     CDS             917..>1112
                     /locus_tag="PKJ43_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010424336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA synthetase"
                     /protein_id="HNZ72913.1"
                     /translation="MQLYKKYLAKAEFDSLDDFLKNFKIVVPENFNFAYDVVDEYARI
                     EPNKIALVWCNDEGQSHNFTY"
BASE COUNT          387 a          225 c          209 g          291 t
ORIGIN      
        1 atcgctcgac gggaaaaaag agcgaacccc agaaataaaa cgaaaagcag tataacaaca
       61 atacctgcca ggccattaga gaatatcttt tggccttttt tagtttcaat ttgaagaaaa
      121 atgtgtgtat tgttctttcc ctaaggcata cgattttgaa taatgcagat gtgagaaact
      181 gaaagatacc aaatactaca gtaatacaac attatatgca gaaaaaagaa tcaattttac
      241 aaaataaacc aggctctagc ttagaataat gcttaaatcg ataaaaaact ctttcaattt
      301 gaaattattt actacttttt cgcctcgttt taaaactaaa attatggaag aacaaataag
      361 gcaaattgct gaacgaattc ggggattacg cgaggttctc gacataagtg ttgaagatgc
      421 cgcaacgacc tgcgggataa gcattgaaaa atatcttgag tatgaatcgg ggaaagttga
      481 catcccagtt agtgtactcc acaacatctc aaagaaatac aatgttgagc ttaccgtttt
      541 gcttaccggc gaagacccgc acatgcacca atattcactc acgcgaaaaa atacaggtgc
      601 agctgttgaa cgaaccaaag cttacaaata tcaatcgtta gctcagggtt tcatcaacaa
      661 aaaggccgag ccgtttattg ttaccgttga gccaaaaccc gaaggctgtt gccagagcct
      721 gaatcaacat cccgggcagg agttcaacta catcctcgaa ggaaagctga agttttgtct
      781 cgacggcaaa gaaatgatcc tcgaagaggg cgattcaatt tatttcaact caggattacg
      841 ccacggaatg cttgccctcg acgaaaaacc ttgtcgcttt ctagccatta ttttttaatc
      901 aaacaatcac ataaccatgc aattatataa gaaatacctt gcaaaagcag aattcgactc
      961 gctcgacgat ttcttaaaaa acttcaaaat agtagttccc gagaatttca attttgcgta
     1021 cgatgttgtg gatgaatatg cccgtatcga gccaaataaa attgccctcg tttggtgtaa
     1081 cgatgaaggc caaagccaca attttacgta tg
//
LOCUS       DAOPUW010000689         1112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_154425_length_1112_cov_2.310312, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000689 DAOPUW010000000
VERSION     DAOPUW010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154425_length_1112_cov_2.310312"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PKJ43_09840"
     CDS             complement(<1..665)
                     /locus_tag="PKJ43_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNZ72914.1"
                     /translation="MEVTRLFDLLENGKKVYSANPALGCKQNGQWITYGISEFIDYAN
                     NLSYGFLAMGLNKGDKVITVTNNRPEWNFVDMGLNQAGMVHVPVYPTISREEYEFILK
                     HSDARLVFVSDKQLYELIKPLADKIPEIIGVFTFNDIPGAPSWMEVVNRGKENSTAMS
                     EKLEAIKPTIQPDDLASMIYTSGTTGMPKGVMLSHKNFLSNAQALLPLFELGPGDKSL
                     SFLP"
     gene            749..>1112
                     /locus_tag="PKJ43_09845"
     CDS             749..>1112
                     /locus_tag="PKJ43_09845"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HNZ72915.1"
                     /translation="MKKITFILIVCSIFFVTNAQQLDAYRIYTANGEEITYGAMKNNL
                     MAKDVVLFGELHDNPIAHWLQLELAASMFETKGKSLTLGAEMFEADNQLIINEYFWDY
                     INQNRFEAEAIAWPRPQTD"
BASE COUNT          321 a          239 c          226 g          326 t
ORIGIN      
        1 ggcaaaaaac ttagagattt atcgcccgga cccaattcga aaagtggtag caatgcctgt
       61 gcattgctga gaaaattttt atgcgaaagc ataacccctt taggcattcc tgtggttccg
      121 gatgtatata tcatcgaagc caaatcgtcg ggctgaatgg taggcttaat ggcttcaagt
      181 ttctccgaca tggccgtgct gttctcttta cccctgttca ccacctccat ccagctagga
      241 gcgccgggaa tatcattaaa agtgaatacg cctatgattt caggaatttt atcggccaat
      301 ggtttaataa gttcataaag ttgcttgtcc gaaacgaata caagccttgc atcggaatgt
      361 ttaaggataa actcgtactc ttccctgctt atggtaggat aaaccggtac atgcaccatc
      421 ccggcctgat tcaggcccat atcaacaaaa ttccactcgg gtcgattatt ggtaacggta
      481 atcaccttat cgcccttgtt gagccccatg gcaagaaagc catagcttaa gttgttggca
      541 taatcaatga attcagaaat gccataagtt atccattgcc cattttgctt acatcccaaa
      601 gcgggattag cgctgtacac cttttttcca ttttcaagca gatcgaataa tcgggttact
      661 tccataatgt tgttttttaa atttttttac tgttgaactc cgatgtcagc caagcagagt
      721 ttttactatt tttacgaaaa acaaaaatat gaaaaagata acttttattt tgatcgtttg
      781 ttcaatattt ttcgtaacaa atgcccaaca gcttgatgca taccgaattt atactgccaa
      841 tggcgaagag ataacttatg gggctatgaa aaacaacctt atggcaaagg atgtagtgct
      901 tttcggagaa cttcacgata atcccatagc ccattggtta cagcttgagt tggctgcaag
      961 tatgttcgag accaaaggaa aatccctaac gttgggagcc gaaatgttcg aggctgataa
     1021 tcaactgatc atcaatgagt acttttggga ttacatcaat cagaatcggt ttgaggccga
     1081 ggccatagcc tggcctcggc ctcaaaccga tt
//
LOCUS       DAOPUW010000690         1108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_155282_length_1108_cov_2.692308, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000690 DAOPUW010000000
VERSION     DAOPUW010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155282_length_1108_cov_2.692308"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PKJ43_09850"
     CDS             <1..628
                     /locus_tag="PKJ43_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HNZ72916.1"
                     /translation="NSTIPVIETGAGICHTYFDESGDVEKGLEIVFNAKARRVSVCNA
                     LDCLIIHENRLNDLPYLLSRMPEANVVVFADEKAYKTLDGKYPAHLLQHATDESFGTE
                     FLDYKMSVKTVAGFDEALEHIATYSSRHSEAIISEDQNCLAMFRKMVDAAAVYTNTST
                     AYTDGAQFGLGAEIGISTQKLHARGPMALEELTSYKWIIEGEGQVRPM"
     gene            767..>1108
                     /locus_tag="PKJ43_09855"
     CDS             767..>1108
                     /locus_tag="PKJ43_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HNZ72917.1"
                     /translation="MRHFTSVHDLPSLEVALSEAFLVKNNPFGFQHLGKNKTMVLMFF
                     NSSLRTRLSTQKAAQNLGMNAIVLDVNQGAWKLETERGVIMDGDKPEHLLEAVPVIGS
                     YADIIGVRSFAS"
BASE COUNT          316 a          210 c          247 g          335 t
ORIGIN      
        1 aaattctacc attcccgtta ttgagacagg cgcaggaatc tgtcatactt atttcgatga
       61 atccggagat gtggaaaagg gtcttgaaat agtttttaat gctaaagccc gaagggtaag
      121 tgtttgtaat gctctcgatt gccttattat tcatgagaat cggttaaacg atctgccgta
      181 tttgcttagc cgtatgcctg aggctaatgt cgtcgttttt gccgatgaaa aagcctataa
      241 aaccctcgat ggaaaatatc cggctcattt actgcaacat gctaccgatg aatcgtttgg
      301 cactgaattt ctcgattaca agatgtcggt gaaaactgta gctggttttg atgaggcact
      361 tgagcatatt gccacttaca gttcaaggca cagtgaagca attatttctg aagatcaaaa
      421 ctgtttggca atgttccgca aaatggtcga tgccgcagca gtatacacca atacatcaac
      481 agcatacact gatggagcac agttcgggct aggggccgaa atcggcattt ctactcaaaa
      541 acttcatgct cgtggtccca tggctcttga agagctaaca tcgtataaat ggattattga
      601 aggcgaagga caagtcaggc caatgtaaaa agttgattat tactaacacg caattagtcg
      661 tttaatcacg atctttagtt gagattttat ttctttgcgt aaaatgcaag acgccgtttt
      721 tccggcaaaa gttttttatt ttcagatttg atatattatt gaaacaatga gacattttac
      781 atcagtacac gatttaccaa gtcttgaagt ggctttgagt gaagctttct tggtgaagaa
      841 taacccgttt ggttttcagc atctgggaaa aaataaaacc atggtgctaa tgttttttaa
      901 ttcgagcctt cgcacccgtt tgagtacgca aaaagctgct caaaacctgg ggatgaatgc
      961 cattgtactc gatgtaaacc aaggtgcttg gaaattagag accgagcggg gtgtgatcat
     1021 ggacggagac aaacccgaac atctccttga ggccgttcca gttattggtt cttatgctga
     1081 tattattggg gtgcgttctt ttgcttca
//
LOCUS       DAOPUW010000691         1106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_155646_length_1106_cov_3.805899, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000691 DAOPUW010000000
VERSION     DAOPUW010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155646_length_1106_cov_3.805899"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..230)
                     /locus_tag="PKJ43_09860"
     CDS             complement(51..230)
                     /locus_tag="PKJ43_09860"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNZ72918.1"
                     /translation="MMNCQYLKVAQQLSVNPNSLYRWVNEYEKYGENAFPGNGSTIYD
                     YQGALHRLEQRKLGT"
     gene            559..>1106
                     /locus_tag="PKJ43_09865"
     CDS             559..>1106
                     /locus_tag="PKJ43_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNZ72919.1"
                     /translation="MVKKDFNYNKICKLEEKILTKRWEIIRKSINHSGEKGFAAEKNP
                     EILNNKPSSHEEAIVFQTLKAILAENPKRWHNTVAELKGVSEETTTGVHRLYQMMEKG
                     ELLFPAINVNDSVTKSKFDNLYGCRESLADGIKRATDVMIAGKVVVVLGYGDVGKGCA
                     KSMRSYGARVIVTEIDPICALQA"
BASE COUNT          344 a          233 c          208 g          321 t
ORIGIN      
        1 cttttggtgc gccgcaagac cttgaatttt ttaaaagctc aacctcttct tcaagttcct
       61 aactttcgtt gttcaagacg gtgtagtgct ccctggtagt catagattgt gcttccattg
      121 ccaggaaatg cattctctcc gtatttctca tactcgttca cccaacgata cagactgttc
      181 ggattcactg aaagctgttg tgctacctta agatactggc aattcatcat cgagcacgag
      241 cttcacagct gcgaccttaa actccttgtt gaacttcttc ctggacatga accctccctt
      301 ttttctctta acttcatgtc cattttatca tacccctcca gaattaagcg cataattggc
      361 ccgaaaagca ggggagcact tcaattaata aaggctatat caaagctcat aatgaaaaac
      421 ctgtttaagt ttaaaatccg ttaataattt gcttgaactt attatattga ttatatttgc
      481 aaaatagtta atattatttt attaggcaat cttataaaat tgaatatatt gagttagcaa
      541 taatagagga tcgtaaatat ggtaaaaaag gactttaatt acaataaaat atgtaagctt
      601 gaggaaaaga tcctaactaa gcgctgggaa ataattagaa agagcattaa ccacagtgga
      661 gaaaaaggat ttgctgccga aaaaaatccc gaaatattga ataacaaacc ttcatcccat
      721 gaagaagcca ttgttttcca aaccttaaaa gccatattag ccgaaaatcc gaaacgttgg
      781 cataatacag ttgccgaatt aaaaggtgtt tccgaagaaa ccaccaccgg tgtgcatcgt
      841 ttgtatcaaa tgatggaaaa aggtgaattg cttttccctg ccatcaatgt gaacgactcg
      901 gttaccaaat cgaaattcga caatctttac ggatgccgcg aatccttggc cgacggtatt
      961 aaacgtgcca ccgatgtgat gatagccgga aaagtagtcg ttgttctcgg ttatggcgat
     1021 gtgggtaaag gctgcgccaa atccatgcgt tcctacggtg caagggtaat cgttaccgaa
     1081 atcgacccca tttgcgccct ccaagc
//
LOCUS       DAOPUW010000692         1106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_155782_length_1106_cov_1.966698, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000692 DAOPUW010000000
VERSION     DAOPUW010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155782_length_1106_cov_1.966698"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PKJ43_09870"
     CDS             complement(<1..717)
                     /locus_tag="PKJ43_09870"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HNZ72920.1"
                     /translation="MSDTREKGQKFKLWFWGIFSFFVVITLSLFIMIGMELFGPLPTF
                     EQLENPKSNIASEVYSEDHVVLGSYYVQFRTFVDYEDISPNIINALLAAEDIRFYRHS
                     AIDARGLARVLVKTIILRHAGSGGGSTITQQLAKNLFPRDTVFYRSPIAKTSNLVVSK
                     FKEWITAVKLERNYTKNELLAMYLNTVAFGSNAYGIRSAAKTFFNTTPDSLNIEQSAL
                     LVGVVNAPTRYSPVKNPERAL"
     gene            complement(889..>1106)
                     /locus_tag="PKJ43_09875"
     CDS             complement(889..>1106)
                     /locus_tag="PKJ43_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72921.1"
                     /translation="VSPKNFPMVFAFAGDSTITKFSMPGIFDSGLMEQKKRHKVKHLQ
                     IELQNFLGQKSKTPLNLTLWENCNSVT"
BASE COUNT          318 a          231 c          235 g          322 t
ORIGIN      
        1 taacgccctt tcgggattct ttaccggact ataccgcgtg ggagcattga caacgccaac
       61 cagtaaggcg gattgctcaa tgttcaggga gtcaggcgtg gtgttgaaaa atgtttttgc
      121 tgccgatcgg atgccgtatg cgttacttcc aaaggcaacg gtattcagat acatggcaag
      181 caactcattt ttagtatagt tgcgctctaa cttaaccgct gtaatccact ctttaaattt
      241 cgacaccacc agattcgagg ttttggcaat gggcgaacgg taaaaaacag tatcgcgagg
      301 aaaaagattt ttggccagct gctgggtgat ggtactccct cctccgcttc cggcatgcct
      361 aagaataatg gttttcacca aaaccctggc taacccacgg gcgtcaatgg cggaatgtcg
      421 gtaaaaacga atatcctctg ctgccagcag tgcgtttatt atgttgggcg aaatatcttc
      481 gtaatcgaca aaagtccgga actgaacgta ataggagccg agcaccacat ggtcctcgga
      541 atacacctcc gaagcaatgt tgctcttagg attttccaac tgctcgaagg tcggaagggg
      601 cccaaacaat tccataccta tcattatgaa aaggctgagg gtaatcacca caaaaaatga
      661 gaaaattccc cagaaccaca gcttaaactt ttggcctttt tcccttgtat cggacataat
      721 ttcaaaaatt tgtttggggt caaaggtagc aataattact tgtctgtaat ccaatgatgc
      781 ttaagaataa aggcaggaat aatttttcaa ttggaatttg acatttttta aaccctatag
      841 gttgcgccag cttagggtaa cctttttacc ataattatat aagcatactt aagtgactga
      901 attacaattt tcccaaagcg tcaaattaag tggtgttttt gatttttgtc ctaaaaaatt
      961 ttgtagttcg atttgcaaat gttttacttt gtgccgtttt ttttgttcca tcaacccact
     1021 atcgaatatg cccggcattg agaacttagt tattgtggaa tcgcccgcaa aggcaaagac
     1081 cattgggaaa tttttaggtg aaacat
//
LOCUS       DAOPUW010000693         1101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_156777_length_1101_cov_3.437859, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000693 DAOPUW010000000
VERSION     DAOPUW010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156777_length_1101_cov_3.437859"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1101
                     /locus_tag="PKJ43_09880"
     CDS             <1..>1101
                     /locus_tag="PKJ43_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72922.1"
                     /translation="LKINKVQLWGIDENFSAFSINSSFPGVKGTEQTCISPNLAQRLN
                     LAVGDEFQLRIEKVSLLPANTPFVSSENQTVTLRVKVGNIVNAEQLGRLNLQNSQTAP
                     YNVFLPIDELNRISEVENKANVLFINTTISKKDIEEAIREVYSIADAGLEIVDASATS
                     EWELRSERVFMDDAVEQLVENTDLEYQPLLTYFANQFQANGRQTPYSFVSSLPDNTLN
                     SNEIVVNEWLASDLKLKPGDSLTLSYYEIGPLRELNEKSKKYIVSRIEPMNGYFADRL
                     LMPDIPGLSDAENCRDWQTGVPINLKTIRKIDEEYWYTYKGLPKAFVSLHEAQELWSN
                     RFGNTTAYRFSQQNISKEELNQIVSEQIDPFN"
BASE COUNT          362 a          248 c          206 g          285 t
ORIGIN      
        1 aactaaaaat taacaaagta cagctttggg gaatcgacga aaatttttct gcatttagca
       61 tcaacagttc attccccggg gtaaaaggaa cagaacaaac atgtatttca ccaaaccttg
      121 cccaacgtct caacctggct gttggtgatg aatttcaact tcgaattgaa aaagttagcc
      181 tgttgcctgc aaatacacct tttgtttctt ctgaaaacca aacagtcaca cttagagtaa
      241 aagtagggaa tattgtcaac gccgaacaat tgggccggtt gaaccttcaa aattcacaaa
      301 ctgctcccta caatgttttc cttcccattg atgaacttaa ccgaatttcg gaagttgaaa
      361 acaaagccaa cgtattattt atcaatacaa ctatatctaa aaaagatatt gaagaagcca
      421 ttcgcgaggt ttactccatc gccgatgccg ggttggaaat agttgatgcc agtgcaacga
      481 gtgaatggga attgcgctcc gaaagggttt ttatggacga tgccgttgaa caattggtcg
      541 aaaacacgga tctcgaatat caacctttgt taacttattt tgcaaaccaa tttcaagcaa
      601 acggtcggca aaccccctac tccttcgtct catctcttcc cgacaataca cttaattcca
      661 acgaaatagt cgtaaatgaa tggctggctt ccgatttgaa actaaaaccc ggcgatagtt
      721 taactctttc ctactatgaa atagggccgc tccgggaact gaacgagaaa tcgaaaaaat
      781 atatcgtgtc tcgtattgag ccaatgaacg gatatttcgc cgaccgattg ctaatgcctg
      841 atattcctgg cctttctgat gctgaaaact gtcgcgactg gcaaaccggg gttcccataa
      901 accttaaaac aatccgaaag atcgacgaag agtattggta cacctacaaa ggattaccta
      961 aagcatttgt ctccttacac gaagcccaag aattatggag caaccgattt ggaaatacaa
     1021 cggcataccg gtttagtcaa caaaacattt caaaagaaga gctcaaccaa atagtatcgg
     1081 aacagatcga cccatttaac t
//
LOCUS       DAOPUW010000694         1095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_158283_length_1095_cov_2.174038, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000694 DAOPUW010000000
VERSION     DAOPUW010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158283_length_1095_cov_2.174038"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PKJ43_09885"
     CDS             <1..239
                     /locus_tag="PKJ43_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72923.1"
                     /translation="CQADLETPSNDSIFPQLYDMPLRAGRMMNEGFHARLRLNRKDNL
                     QAFENTIEEGLLTALNDKAFDEWIAGKSVFKKIS"
     gene            295..>1095
                     /locus_tag="PKJ43_09890"
     CDS             295..>1095
                     /locus_tag="PKJ43_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HNZ72924.1"
                     /translation="MKVIIDNKIPYIQGALEPFAEVIYLPGKDTTAEIAKDADAIITR
                     TRTKCNEELLAGSTVKMIATATIGYDHIDTKWCEANGIEWKNAPGCNSWSVVQYIMAG
                     LHTLALEKKMKLEGMTIGIIGAGNVGSKVAKYCQLIGMNVLVNDPPRQRAEGNDGFVS
                     LDEIQEKAEIITIHTPLTYEGADKTFHLIDEKFIDSCSQDFFLINAARGEVTDTHAVL
                     TALKFGKIREAIIDCWEKEPEIDLELLEHTFISTPHIAGYSRDGKANGT"
BASE COUNT          367 a          244 c          240 g          244 t
ORIGIN      
        1 gttgccaggc cgatctggaa acccctagca atgattcgat tttcccgcaa ctttacgata
       61 tgccgttgcg tgctggccgc atgatgaacg aaggattcca cgcccgactt cgtttaaaca
      121 gaaaggacaa ccttcaggct ttcgaaaata ccattgaaga aggcttgctc accgcattga
      181 acgacaaagc ctttgatgag tggattgccg ggaaaagtgt tttcaaaaaa ataagctaag
      241 caacaagaac aacttcacaa cttttcgata tacaaaacag acaataagaa acatatgaaa
      301 gtaataatcg acaataaaat cccctacata caaggagcct tggagccttt tgccgaagta
      361 atttacctcc cgggaaaaga taccaccgcc gaaattgcca aagatgccga tgcaattatt
      421 acgcgtaccc gaacaaaatg caacgaagaa cttcttgccg ggtcaactgt aaaaatgatt
      481 gcaaccgcaa caattggcta tgaccacatc gacaccaaat ggtgcgaagc caacggaatt
      541 gagtggaaaa atgcacccgg ctgcaacagc tggtcggtag tacaatatat aatggccgga
      601 ctgcatacac tggcactcga gaagaaaatg aaacttgaag gaatgaccat tggcattatt
      661 ggcgccggaa acgttggctc gaaagtggca aaatattgtc aacttatcgg gatgaacgta
      721 ctcgttaacg accctccccg ccagcgggct gaagggaacg atgggtttgt ttcgctagat
      781 gaaattcagg aaaaagctga aatcataacg attcatactc cgttaaccta cgaaggagca
      841 gataaaacat tccacctgat cgatgagaaa tttatcgatt cgtgcagcca agactttttt
      901 ctaattaacg cagcacgagg agaagttaca gatactcacg cagttcttac tgccctgaaa
      961 tttgggaaaa taagagaagc cataatcgac tgttgggaaa aagagcccga aattgacttg
     1021 gaattgctgg aacatacatt tatttcaact cctcacatag ctgggtattc gcgcgacgga
     1081 aaagccaatg gtaca
//
LOCUS       DAOPUW010000695         1092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_159024_length_1092_cov_2.098361, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000695 DAOPUW010000000
VERSION     DAOPUW010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159024_length_1092_cov_2.098361"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="PKJ43_09895"
     CDS             <1..951
                     /locus_tag="PKJ43_09895"
                     /inference="COORDINATES: protein motif:HMM:NF016159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HNZ72925.1"
                     /translation="TQISDEEILGILKTYFLQPFRQPEFYSFSHETDLSFNEVYKFAE
                     KLFENPDNLFETSVSIAKHLFQSSNHPNIKDGELYVVLFTHCVVEDELVDAIGIFKSE
                     TKDTFIKIYEKQDGFELEQHQGISIRKLDKGCLIFNTEKDFGFKVVAVDNTNANDEAR
                     FWKEIFLNIKRREDDFYQTSGYINLCKDFVKDVLYHDGSMDRAEQVTLLNKTRDYFAQ
                     TDIFNRDEFEERVIRKPEIVDAFREYKNEYQQELGCDLKDSFTISIDAAKQAKKLFKS
                     VIKLDKNFHLYIHGGRDRVEKGFDRDKGLNYYKLYFDAEG"
BASE COUNT          345 a          215 c          255 g          277 t
ORIGIN      
        1 acacaaattt ccgacgagga aatattgggc attctgaaaa catactttct tcaaccgttc
       61 aggcagcccg agttttacag cttctcccac gaaactgacc tctccttcaa cgaggtatat
      121 aaatttgccg aaaagctgtt tgaaaacccc gataatcttt tcgaaacatc tgtcagcatt
      181 gccaaacatc tgttccagag cagtaatcat cccaacataa aggatggcga actatacgtc
      241 gtactcttca cccactgcgt tgtggaggat gaactggttg atgccatagg gattttcaag
      301 tcggaaacca aggatacatt tatcaagatt tacgaaaaac aggacggatt tgaattggaa
      361 caacatcagg gaattagcat taggaaactg gacaagggat gcctgatatt caataccgaa
      421 aaggattttg gtttcaaggt ggtggctgtt gataatacca atgccaacga cgaggcgcgg
      481 ttttggaaag aaatatttct taacatcaaa cgcagggagg atgactttta ccagaccagt
      541 ggctatatca atctttgcaa agattttgta aaggatgtgc tttaccacga cggctccatg
      601 gacagggccg agcaggttac cctgctcaat aaaacccgcg attactttgc tcaaaccgat
      661 atctttaatc gcgacgaatt tgaggaaagg gtaatacgca aaccggaaat tgtggatgct
      721 tttcgtgagt ataaaaatga atatcagcag gaactgggct gcgacctcaa ggattcgttc
      781 accatttcca ttgatgctgc caaacaggcc aaaaaactgt ttaaaagcgt gataaaatta
      841 gacaagaatt ttcatcttta cattcacggt ggccgcgata gggttgaaaa gggtttcgac
      901 cgcgacaagg gattgaatta ctacaaactt tatttcgatg ccgaggggta gaattgttaa
      961 agaaaaaccg aaaggataaa gcataagcgc atggaagagg atattaacaa actggtgttc
     1021 agcaggtctg tggttgagtt tgcaacggtt gccaaggagt attgcgcata cctggagggc
     1081 attggagggt ac
//
LOCUS       DAOPUW010000696         1092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_159030_length_1092_cov_2.065574, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000696 DAOPUW010000000
VERSION     DAOPUW010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159030_length_1092_cov_2.065574"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PKJ43_09900"
     CDS             complement(<1..788)
                     /locus_tag="PKJ43_09900"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HNZ72926.1"
                     /translation="MNIPEKAVGVVAFGLKTGIITGDDNIVDVVRKCLINNPDIIREN
                     DIICITEAVVAITQHNIVQLDDVSMEIKAKLNLSDNSTLGVIFPILSRNRFSMLLKAM
                     AKAVPKGKVIIQLMFPADEQGNPIVAKSTLRDLKKHSEDLITVDELGENRLLHPETGL
                     DYIDYYTEIVEANSAKADIYLANRVEYMIGKKPDAVIVSNVHARYEVWEEFLKMGYKN
                     VITLQDICSTPGKGAHSEFGLLGSNILDPAHEQLKLCPVHPNEV"
BASE COUNT          332 a          254 c          192 g          314 t
ORIGIN      
        1 catacctcat ttggatgcac ggggcacagt ttaagctgtt cgtgtgcagg gtcgagaatg
       61 ttcgatccca gcaatccaaa ttccgaatga gctcccttac cgggagtaga acagatatcc
      121 tgcaacgtaa tgacgttttt atatcccatc ttcagaaact cttcccaaac ctcgtaccgg
      181 gcatgaacat tgctcacgat cacggcatcg ggtttcttgc caatcatgta ttcaaccctg
      241 ttggccaggt aaatatctgc cttggcagag tttgcctcaa caatttcggt ataataatca
      301 atataatcca accctgtctc gggatggagc agcctatttt cgccaagttc gtccacagtt
      361 atcaaatcct cggaatgttt tttcaaatcc ctcagggttg attttgccac tatgggattt
      421 ccctgttcat cagcagggaa catcagctgg attatcacct tccctttagg cactgcttta
      481 gccatagctt tgagcagcat cgaaaagcga tttcgcgaaa ggattggaaa aataacacct
      541 aaagttgaat tatcgctaag gttcaacttg gctttaatct ccatcgacac atcgtcgagt
      601 tgaacaatgt tgtgctgcgt aatggcaact actgcctcgg taatgcagat gatatcattc
      661 tcccgaatga tatcggggtt gttaatcaaa catttcctta caacatcaac aatgttatca
      721 tcaccggtta ttattccggt ttttaagcca aaggccacaa ctccaactgc cttttcagga
      781 atattcatct atgctatact tttgattacc tgtggtttaa tcaaaacatt attttgtttt
      841 gattttaaca aagtaacgaa aaaacaacag cattcctaca aaaataccgt acaggttacc
      901 acgttttttt aaacaactaa ttggcatgga ttatctgata tggtaaaagg gcataaaaaa
      961 gaagccggac ttccccaagc ccggcttcac accctaaaaa attaacctaa aacaactaac
     1021 ccgaaaccaa ctaaaaccct aatgaaaaga aaaactcgtt aagtttcatt agtaaaaacg
     1081 taactcatcg tt
//
LOCUS       DAOPUW010000697         1091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_159153_length_1091_cov_3.925676, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000697 DAOPUW010000000
VERSION     DAOPUW010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159153_length_1091_cov_3.925676"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1091)
                     /locus_tag="PKJ43_09905"
     CDS             complement(<1..>1091)
                     /locus_tag="PKJ43_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HNZ72927.1"
                     /translation="LAFDTLYAEGQRRYVESLSSYARQFLGRINKPEVDFIKGIPPAI
                     AIEQKVNTRNPRSTVGTSTEIYDYLKLHYARIGKTISPISGKEVKKNDVTDVVDYMQS
                     FNKGTRVLVLAPFSVRKGRKAEEEIGMLYQQGYTRYELNYATYSADSPELLELLPKQA
                     KNLNIVIDRIAVNDDDDTLSRMSDSVQSAFNEGNGVCIIKNIDGEPSSASFSHCFEMD
                     GIRFEEPSVDMFSFNNPLGACPRCEGYGKVIGIDEDLVIPNKMLSIYDDAVACWKGDK
                     MSEWKNQLIENAYRFNFPVHKPYYELSTEQQELIWTGNKWFGGIDQFFKFVEENSYKI
                     QYRVMLARYRGKTICPECKGSRLKKESGYV"
BASE COUNT          256 a          245 c          231 g          359 t
ORIGIN      
        1 acatatcccg attccttttt taaccggctt cctttgcact ccggacaaat tgtttttccc
       61 cggtatcggg ctagcataac ccggtattgt atcttataac tgttttcttc aacaaatttg
      121 aaaaactggt cgattccccc aaaccattta ttgcccgtcc aaataagttc ttgttgttcg
      181 gtgcttagtt cataataggg tttgtgaaca ggaaaattga agcggtatgc attttctatt
      241 aactggtttt tccattcact cattttgtct cccttccagc atgccactgc atcgtcgtat
      301 atcgaaagca ttttattggg aataaccagg tcttcgtcaa ttccaattac ttttccgtac
      361 ccttcacatc gcgggcatgc cccaagagga ttgttgaaac tgaacatatc taccgaaggt
      421 tcttcaaacc ggatgccatc catttcgaag caatggctga aagaagctga cgatggttct
      481 ccatcgatat ttttaataat gcatacgcca ttgccttcgt tgaatgccga ctgtactgag
      541 tctgacatcc tgctcagcgt atcgtcatcg tcgttcactg ctattcggtc aatgacgatg
      601 ttcaggtttt tggcttgctt aggcagaagt tcgagcagtt ccggcgagtc ggctgagtag
      661 gtggcatagt tgagctcgta acgggtgtaa ccttgctgat atagcattcc tatttcttct
      721 tcagcttttc tccctttcct tacgctaaac ggggcaagaa caagtactct ggttcctttg
      781 ttgaacgatt gcatgtagtc gaccacatcg gtaacatcat tttttttaac ttctttgccg
      841 gaaattggag aaatggtttt cccaatccgg gcataatgga gctttaagta atcgtagatt
      901 tcggtagatg ttcctacggt tgatcgtggg ttacgggtgt ttactttttg ttcgatggca
      961 attgccggtg gaattccttt gatgaaatca acttcgggtt tgttaatcct ccctagaaat
     1021 tggcgggcat acgatgataa actctcaacg tagcgccgtt gaccttcagc atataatgtg
     1081 tcgaaagcca g
//
LOCUS       DAOPUW010000698         1091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_159157_length_1091_cov_3.640927, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000698 DAOPUW010000000
VERSION     DAOPUW010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159157_length_1091_cov_3.640927"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..>1091
                     /locus_tag="PKJ43_09910"
     CDS             61..>1091
                     /locus_tag="PKJ43_09910"
                     /inference="COORDINATES: protein motif:HMM:NF027309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HNZ72928.1"
                     /translation="MEHQRKSFTLRRVVNILAVIGLILSSSLGSRAIASAPVADDDKN
                     IQKFTISEKKNSADFILAEKGNVASLVVSAFDFPGVIEVMRMFQQDVEAVTGVEPKLL
                     LDAMPQSKQAVIAGTLGKSTLIDQLIAAGKINVDDVRNKWENSLIQVVDNPFPNIERA
                     LVIVGSDKRGTMYGILEVSKQIGVSPWYWWADVPVESKQNLYVKPGRFNFGEPKVKYR
                     GIFLNDEEPALGRWAVENYGGFNHQFYEKVFELLLRLKGNYLWPAMWWAGFNMDDPQN
                     AEMADKYGIVMGTSHHEPLNRAQAEWRRYGKGAWNYETNSAELTKFWREGIERIGNKE
                     VVVNMAMRG"
BASE COUNT          330 a          189 c          272 g          300 t
ORIGIN      
        1 acacttgtct aatcatatga aatgatttat ctttctgctt caattttaaa tgttaaaaag
       61 atggaacatc aaagaaaatc cttcacgtta agaagagttg taaatatact ggctgttatt
      121 ggcttaatac tctcttcttc acttggcagt agggcaatcg cttcggcacc tgtggctgat
      181 gacgataaaa acattcagaa atttaccatt tcagaaaaga aaaattcagc cgattttatt
      241 cttgctgaaa agggaaatgt agcttccttg gttgttagtg ccttcgattt tcccggcgtt
      301 attgaagtga tgaggatgtt ccaacaagat gttgaggcgg ttacaggtgt tgagccaaaa
      361 ctgctgctcg atgcaatgcc tcaaagtaaa caagcggtta tcgctggtac attgggaaaa
      421 agtacactta tcgaccagct aatagctgca gggaaaatca atgtcgatga cgttcgaaat
      481 aaatgggaaa attcacttat tcaggttgtt gataatccat ttcccaatat cgaaagggcc
      541 ctggtaattg ttggaagcga caagcgcggc acgatgtatg gcatactgga agtgtcgaaa
      601 caaattgggg tgtcgccatg gtattggtgg gccgatgttc cggtagaatc gaaacaaaat
      661 ttatatgtaa aacccgggcg gtttaatttt ggtgagccaa aggtaaaata caggggtatt
      721 ttcctaaacg atgaagaacc tgcattagga cgctgggcag tagaaaatta cggtggcttt
      781 aatcatcaat tctacgagaa ggttttcgaa ttgttgctcc gcttgaaagg aaactatctt
      841 tggccggcca tgtggtgggc tgggttcaat atggatgacc ctcaaaatgc cgaaatggct
      901 gataaatacg gtatcgttat gggaacgtca caccacgaac cgctgaatcg ggcgcaagcc
      961 gaatggcgtc gttacgggaa aggggcctgg aattatgaaa ctaatagtgc cgaattgaca
     1021 aaattttgga gagaaggcat agagcgtatt ggcaataaag aagttgttgt taatatggct
     1081 atgcgtggcg a
//
LOCUS       DAOPUW010000699         1090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_159419_length_1090_cov_2.594203, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000699 DAOPUW010000000
VERSION     DAOPUW010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159419_length_1090_cov_2.594203"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PKJ43_09915"
     CDS             complement(<1..636)
                     /locus_tag="PKJ43_09915"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72929.1"
                     /translation="MKPKKYYILIISILLFVRANAQTVSEVVAFADTEFEKGNYEIAA
                     REYNRALFFGSENVDVISLQIGHCYSEMEEYALAAEFYDRAYKYSQNDSLKNESVVAK
                     AFGMLVTNQYIKALEELLYFSENSSPEQKTQMHYLKGIAYYNTENDSLACVEFLQALE
                     VFPSSDSLKQLITQEFTNVQRYPKRYNPNRAYIMSAFFPGSGQFSVGAVKEG"
     gene            complement(602..>1090)
                     /locus_tag="PKJ43_09920"
     CDS             complement(602..>1090)
                     /locus_tag="PKJ43_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72930.1"
                     /translation="TVKQLITDGFDFSQKTEHQLGTALLLRNWEEAYQLSLKDKPENN
                     YKLTGLQGIAARSYSAKRKSPALAAIMSAIIPGAGKAYCGYWGDGVISFLFSASTTFF
                     AYRGFEKYGTKSVYPWIVGGLAFSYYSSNIYGGAASAKRYNTNLDHSFIHETEKILYS
                     DY"
BASE COUNT          294 a          222 c          228 g          346 t
ORIGIN      
        1 tccctcttta acagctccta cagaaaattg tcccgatcca ggaaaaaaag cactcattat
       61 gtaggctcga tttgggttgt agcgtttagg gtatctttgt acattggtga attcttgagt
      121 gatcagctgt ttcaaagaat ctgatgatgg aaagacttca agagcttgca aaaattcgac
      181 acaggcaagc gagtcatttt cagtgttata gtaagcaatt cctttcagat agtgcatttg
      241 ggttttttgt tctggagagg agttttcact aaaataaagt aattcttcga gtgcttttat
      301 gtattggttt gtaaccaaca taccgaaggc tttggccaca accgattcat tttttaagct
      361 atcgttctgg ctgtatttat aggctcggtc gtaaaattcg gcagcaaggg catattcttc
      421 catttcagaa tagcagtgcc caatttgaag tgaaattaca tcgacatttt ccgatccgaa
      481 aaacaaggct cggttatatt cacgggctgc aatttcgtag ttcccttttt caaattcggt
      541 atcggcaaag gcgaccactt ctgaaaccgt ttgagcattt gccctaacga agagcaaaat
      601 gctaataatc agaatataat attttttcgg tttcatgtat aaatgaatga tcgaggtttg
      661 tattatagcg tttggctgat gctgcgcctc cataaatgtt cgatgaataa tagctaaatg
      721 ccaatcctcc aactatccac gggtatacac ttttagttcc gtatttctcg aaacctctgt
      781 aggcaaaaaa tgtagtagat gcgctaaaca gaaacgaaat taccccatca ccccagtacc
      841 cgcaataggc tttccccgca ccgggaatta tagccgacat gatagcggcc agtgctgggc
      901 tcttcctttt agccgaatag cttcttgctg ctatgccctg aagtccagtt agtttatagt
      961 tgttttcggg cttgtcttta aggctaagct gataggcctc ttcccagttt cgaagcaata
     1021 aggctgtgcc aagttggtgt tctgtttttt ggctaaagtc gaaaccatcg gtaatcagct
     1081 gttttactgt
//
LOCUS       DAOPUW010000700         1090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_159434_length_1090_cov_2.429952, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000700 DAOPUW010000000
VERSION     DAOPUW010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159434_length_1090_cov_2.429952"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PKJ43_09925"
     CDS             complement(<1..685)
                     /locus_tag="PKJ43_09925"
                     /inference="COORDINATES: protein motif:HMM:NF013166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNZ72931.1"
                     /translation="MKLFYREWGRGEPLLILHGLYGCSDNWMTIARKLAERFQVIAVD
                     LRNHGNSPHADSHTYTDMSLDLEELFQLLQINSAHIIGHSMGGKVAMAFAADFPERIR
                     SLTVVDIAPKNYLLYPNGTSQYEKHKQILDTFACIDLSNFSSRVEVEEVMKSRINDTF
                     LALYLLKNLKRLPAGFSWKINVPVLQNYLLSIVSDVNADFFESRLPIFNYPVLFVKGA
                     LSEYISSADE"
     gene            complement(682..>1090)
                     /locus_tag="PKJ43_09930"
     CDS             complement(682..>1090)
                     /locus_tag="PKJ43_09930"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF015685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNZ72932.1"
                     /translation="WDTVANKVFLSEVIAEFKSKGIRVSVFVGTELRFIEGAKRCGAD
                     RVELYTEPYAKLFPVNAEAAARPFVAAANFANELGLGVNAGHDLDLHNLSYLNAQIPF
                     LAEVSIGHALISDALYLGLETTIKKYIACLIRQ"
BASE COUNT          365 a          235 c          213 g          277 t
ORIGIN      
        1 actcgtcggc tgaactaata tattcagata atgcgccttt tacaaacaga accgggtaat
       61 tgaaaattgg aagcctcgat tcgaagaaat cagcattaac gtcggataca atagataata
      121 aatagttctg aagaacggga acattaatct tccaggaaaa accagcaggc aaacgtttaa
      181 ggtttttcaa taagtataac gctaaaaaag tgtcattgat acgtgatttc ataacctctt
      241 caacttcaac cctagaagaa aaattactca gatcgataca tgcgaaagta tcaagaatct
      301 gtttatgctt ctcgtactga gaagtgccgt tgggataaag caaataattc tttggggcaa
      361 tatcaactac agtcaatgag cggattcttt ccggaaaatc agcagcaaaa gccatggcaa
      421 cttttccgcc catgctatga ccaataatat gtgcagaatt gatttgaagc aattgaaaca
      481 actcttcgag atcgagagac atgtcagtat aagtatgaga atcagcatgt ggcgagttcc
      541 catggttcct caaatcaaca gcaataactt gaaaacgttc agccaatttt cgagcaatag
      601 tcatccaatt atcggagcat ccatataacc cgtgaagaat cagtaaaggc tcacccctgc
      661 cccattccct gtagaatagt ttcattggcg aataagacag gcaatatatt ttttaatcgt
      721 agtttcgaga cctaaataca gcgcgtcgga tattagcgca tgcccgatcg aaacttctgc
      781 aaggaaagga atttgagcat ttagataact aagattgtgt aaatcgagat cgtggccagc
      841 attaacgcca aggccaagtt cattggcaaa gtttgcagcc gcaacaaaag gcctggctgc
      901 tgcctcagca tttacaggaa atagctttgc ataaggctcg gtatacagct caacccgatc
      961 ggctccacag cgttttgccc cttcgataaa acgtaattca gtgccaacaa aaacagaaac
     1021 cctaatccct ttcgatttga actcggcaat aacttctgaa aggaaaacct tgttggcaac
     1081 tgtatcccac
//
LOCUS       DAOPUW010000701         1089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_159601_length_1089_cov_3.041586, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000701 DAOPUW010000000
VERSION     DAOPUW010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159601_length_1089_cov_3.041586"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1089)
                     /locus_tag="PKJ43_09935"
     CDS             complement(<1..>1089)
                     /locus_tag="PKJ43_09935"
                     /inference="COORDINATES: protein motif:HMM:NF028498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HNZ72933.1"
                     /translation="FGGIHINAWTGQQLNNDMCEKAFDNDPGEIGLSIFRIWVDPEKN
                     GWNAELPAAKYAVSKGAIVFASPWNPPSNMKEELRKTDQGTDYRLLPQYYGAYTDHLN
                     SFIDHMNSNEVPLYAISVQNEPDWHSWTWWEPNEMLKFVRENAQNINCRVIAPESFSY
                     SRKMIDPLLNDSIANSNIDILGTHLYGTPKSNFYYPLAYQKGKEIWMTEHLYGSDKPA
                     DNTWELALQVAEEINTCMDARMSAFVYWYIRRFYGLIDDSGNITDKGYVMSHFSKFVR
                     PGSYRVEMPFNAGTNITATAYKNDSTLAIVVVNRSNNAATLNFNIENNLDVIDSMTQF
                     TTTALKKVNNEGTFTIENGSFSASVEPKS"
BASE COUNT          262 a          234 c          239 g          354 t
ORIGIN      
        1 gcttttgggt tcaacacttg ccgaaaagct tccgttttca atggtaaatg ttccctcgtt
       61 gtttactttt ttcaaggctg tagtagtaaa ttgagtcatt gaatcgatca cgtcgaggtt
      121 gttctcgata ttaaaattaa gtgtggctgc attgttgcta cggttgacaa ccacaattgc
      181 cagcgttgag tcgtttttgt aagcagttgc tgtaatattg gttccggcat tgaaaggcat
      241 ttcaacccga tatgagccgg gcctgacaaa ttttgaaaaa tgcgacatta catacccttt
      301 gtcggtaata ttgcccgaat catcaataag tccataaaaa cggcgaatat accaatacac
      361 aaacgcgctc atgcgggcat ccatgcatgt atttatttct tccgccacct gaagggctag
      421 ctcccatgtg ttgtcggctg gtttgtcgct tccgtataag tgttcggtca tccatatttc
      481 ttttcctttc tgataggcca gaggatagta gaaattcgat tttggcgttc cgtagagatg
      541 agttcccaga atgtcgatat ttgaattggc aatagagtcg tttaataacg ggtcgatcat
      601 ttttcggctg tagctaaacg attcgggagc aataaccctg caatttatat tttgggcgtt
      661 ttcgcgcaca aatttcaaca tttcattggg ttcccaccat gtccagctat gccagtcggg
      721 ttcgttttgt accgaaatgg catacagggg cacctcatta ctattcatgt gatcgatgaa
      781 gctgttgaga tgatcggtat atgctccata gtattggggc agaagtctgt agtcggtccc
      841 ttgatcggtt tttctaagct cttctttcat gttagaaggg ggattccaag gcgatgcaaa
      901 cacaatagcc cctttactta ctgcatattt tgcagccggc aattcagcat tccacccgtt
      961 tttttctggg tcgacccaaa tacggaaaat gctcagtcct atttcgccag gatcattgtc
     1021 gaatgctttt tcgcacatgt cgttgttcag ttgctgacct gtccatgcgt ttatatgaat
     1081 tcctccaaa
//
LOCUS       DAOPUW010000702         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_160657_length_1085_cov_2.176699, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000702 DAOPUW010000000
VERSION     DAOPUW010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160657_length_1085_cov_2.176699"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PKJ43_09940"
     CDS             complement(<1..358)
                     /locus_tag="PKJ43_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HNZ72934.1"
                     /translation="MKKLVVFFIALTVFTGLHAQNSIVLQPNKAQTVINKNIYGHFSE
                     HLGTCIYGGIYVGKDSKIPNIDGYRTDVVNALKEMKIPVLRWPGGCFADTYHWKDGIG
                     PKENRPSIVNVHWGGVT"
     gene            complement(382..>1085)
                     /locus_tag="PKJ43_09945"
     CDS             complement(382..>1085)
                     /locus_tag="PKJ43_09945"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNZ72935.1"
                     /translation="NLIRFIPSIPEHIYAQNSNNLYVNLFISSKAQISLDKQALNVEL
                     QTNYPYHGKITLHVSPAKPSTFNLNLRIPGWARNEVTPGDLYSYRSNSTDNIVLKVNG
                     ENEPLVMKNGYAVISRKWKKGDIVSVELPIEVKQVIANEIVEEDRDKIAFEYGPLVYC
                     AEEADNGTLNEMKIPADISFNIEDFSVLDEKTKALTTNINGKTLKLIPYFAWSNRGIG
                     EMKVWFDRDKNETNN"
BASE COUNT          280 a          217 c          204 g          384 t
ORIGIN      
        1 cggtaacacc gccccagtga acattcacaa tggatggacg gttttctttt ggcccaatac
       61 catctttcca atggtaggtg tcggcaaaac agccgcctgg ccaacgtaat accgggattt
      121 tcatctcttt caatgcattt accacatcgg tgcggtaccc gtcgatgttt ggaattttcg
      181 agtctttacc aacgtaaatt cctccgtaga tgcatgttcc caagtgttct gaaaaatgcc
      241 cgtaaatgtt tttgttgatt accgtttggg ctttattggg ttgaagcaca atgctgtttt
      301 gtgcatgcag ccccgtaaat acggtaagtg caataaaaaa tacgactaac tttttcataa
      361 gctttctgat tttattgata attaattatt tgtttcattt ttgtctctgt cgaaccatac
      421 tttcatttct ccaattcccc ggttcgacca tgcaaaatag gggatgagtt taagcgtttt
      481 tccattgata tttgtggtta acgcttttgt tttctcatcc aatacgctga aatcttcaat
      541 gttgaaactg atgtcagctg ggattttcat ttcattcaat gtgccattat ctgcttcttc
      601 agcacaatat actaacggtc cgtattcgaa agcaatttta tcacgatctt cttcaactat
      661 ttcattcgca ataacttgtt ttacttcaat aggtaattca acacttacaa tatctccttt
      721 tttccatttt ctggagatta ctgcatatcc atttttcatc accaatggtt cattttcccc
      781 attcactttc agaacgatgt tatcggttga attagaacga tatgaataca agtcgcctgg
      841 agttacttca ttacgtgccc aacctggaat tcttaggttc aggttaaagg ttgagggttt
      901 tgctggcgaa acatgtagcg taatttttcc atgataaggg taattggtct gtagctcaac
      961 attcaatgct tgcttgtcga ggctgatttg tgctttgctc gaaatgaata aattgacata
     1021 caggttattg ctattttgag cataaatgtg ttcaggaatc gacgggataa aacggataag
     1081 gtttg
//
LOCUS       DAOPUW010000703         1084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_160841_length_1084_cov_2.913508, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000703 DAOPUW010000000
VERSION     DAOPUW010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160841_length_1084_cov_2.913508"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PKJ43_09950"
     CDS             <1..306
                     /locus_tag="PKJ43_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNZ72936.1"
                     /translation="PKLLEPVYDVVVMTPSDRLGDVMSDLQSRRAVIMGMNSEKGFEK
                     LQAKIPLKELSNYSTTLSSLTGGRASFVMKFASYELVPIEIQEKLLKEYEASKKEED"
     gene            complement(391..>1084)
                     /locus_tag="PKJ43_09955"
     CDS             complement(391..>1084)
                     /locus_tag="PKJ43_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ72937.1"
                     /translation="EKINAKIIPADATNLDDLEQVFSQSMEILGGKIDFILHSIGMSP
                     NVRKKRPYDDLDYDLMHTTLDISAISFHKMLQTAKKMNAINEWGSVVALSYIASHRTM
                     YGYNDMADAKAMLESIARNFGYIYGREHNVRINTISQSPTPTTAGSGVKGFDSLMDFS
                     ERMSPLGNASADDCANYCVVMFSDLTRKVTMQNLFHDGGFSSMGMSYRALQQYNKSFE
                     GSQTHDGKIIYG"
BASE COUNT          319 a          206 c          228 g          331 t
ORIGIN      
        1 cctaaattgc tggaaccggt ttatgatgtg gtggtaatga ctccatcgga caggttggga
       61 gatgtgatga gcgatttgca aagccgtcgt gccgttatca tgggtatgaa cagcgaaaaa
      121 ggttttgaaa agttgcaagc aaaaattcct ttgaaagaac tgagtaatta ttccacaacc
      181 ttgagttctt taaccggtgg aagagcttcg tttgtaatga aatttgcaag ttatgaactc
      241 gttcctatcg aaattcagga aaaactgttg aaagaatatg aagcttctaa gaaagaagaa
      301 gattaacttc taaattgaat tttaaaaaaa ggggcaattt actttgaaag tagattgtcc
      361 ctttttagtt ttataattca atttgttgga ttaaccgtaa ataattttgc catcgtgagt
      421 ttgacttcct tcaaagcttt tgttgtactg ctgcaatgca cgataactca tacccatgct
      481 cgaaaagcct ccatcgtgga aaaggttttg catggttacc ttgcgtgtca ggtcgctaaa
      541 cattactaca caataattgg cgcaatcatc tgctgaagcg tttcccagtg gcgacatgcg
      601 ttccgaaaaa tccattaaac tgtcaaatcc cttcaccccg cttcctgcag tagtaggagt
      661 tggcgactga gaaatagtgt tgatacgcac attgtgttct cttccgtaaa tgtatccaaa
      721 gtttcgggct atcgattcca gcatggcttt ggcatcggcc atgtcgttgt atccatacat
      781 cgttcgatga gaagcaatgt aggaaagtgc aacaaccgat ccccactcat tgatggcatt
      841 catcttttta gcagtctgaa gcattttgtg aaaagatatg gctgaaatat ccaatgtggt
      901 gtgcatcaaa tcataatcca aatcatcata gggcctcttt ttcctcacat ttggtgacat
      961 gccaatggaa tggagaataa agtcgatttt accacccaaa atttccatcg attgagagaa
     1021 aacttgctcc aaatcatcaa gattggtagc atcggcgggg ataattttgg cattgatttt
     1081 ttct
//
LOCUS       DAOPUW010000704         1082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_161279_length_1082_cov_3.185979, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000704 DAOPUW010000000
VERSION     DAOPUW010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161279_length_1082_cov_3.185979"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PKJ43_09960"
     CDS             <1..386
                     /locus_tag="PKJ43_09960"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNZ72938.1"
                     /translation="CKRNWLRNTHLSLNLFLYGLPTILYLPLANFETLGNISFEWWLL
                     LLFLGANTLIAYGCMNASLKYIDANKVSALLVNNPIITFIIMAILSWKQVSWIPAEHF
                     SILAWAGALMFIFGAFMVVKKGNKN"
     gene            439..>1082
                     /locus_tag="PKJ43_09965"
     CDS             439..>1082
                     /locus_tag="PKJ43_09965"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNZ72939.1"
                     /translation="MEKPVYYIDCILPVPLNQYFTYRISEKIADKAKVGCRVIVQFGN
                     RKYYTAIILQIHTNKPEYETKETETLLDDEPIVGLHSFSFWEWMAAYYCCPVGDVMNA
                     ALPSGLKLESHTSVHINQDWIEYERLSGIEESVFLFLQGQEHATIQQINSLTKKTNAY
                     SIIQSLLKKDAIRIEEKVEEKYQPKLVPYLKISSTLICENDFNIAFENLKKAKK"
BASE COUNT          343 a          227 c          201 g          311 t
ORIGIN      
        1 aatgcaaaag aaactggtta cgaaataccc acctctcgct taaccttttt ttatacggac
       61 ttccaacaat tttatacctt ccgttagcca attttgaaac gttgggaaat atttcatttg
      121 aatggtggtt gcttttactt tttcttggtg cgaatacact tattgcctat ggctgtatga
      181 atgcttcgct taagtatatc gatgcaaaca aggtaagtgc cttacttgtt aacaacccta
      241 ttatcacctt cataatcatg gccattcttt catggaaaca agtttcatgg atacccgctg
      301 aacacttcag catattggca tgggccggtg cgctcatgtt tatttttggc gctttcatgg
      361 tcgtaaaaaa agggaataaa aattagcgct caacaacgcg tttaaaatag tgagacgtat
      421 atttgtgcaa attagcacat ggaaaagcct gtttactata tcgactgtat attaccggta
      481 ccactcaacc aatactttac ttaccggatt tccgaaaaaa ttgcagataa agcaaaagta
      541 ggctgccgtg tcattgtaca atttggaaac cgcaaatatt acacggctat cattctccaa
      601 attcatacca acaaaccgga atacgaaacc aaagaaacag agaccctgct cgacgatgaa
      661 ccaatagttg gtcttcattc tttttctttc tgggaatgga tggcagcgta ttattgctgt
      721 ccggttggcg atgtaatgaa tgccgcgtta ccctccgggt taaaacttga aagccacacc
      781 tcggtacata ttaaccagga ttggattgag tacgaacgcc tttcgggcat tgaagagtcg
      841 gtttttttgt tcctgcaagg tcaggaacat gctaccatac agcaaatcaa ctcactgaca
      901 aaaaaaacga atgcatattc tatcattcaa tctctcctga agaaagatgc cattcgaatt
      961 gaagaaaaag tcgaggaaaa gtatcagccc aaactggtcc cctacctgaa aatatcatca
     1021 acgctgattt gtgaaaatga tttcaacata gcgtttgaaa acctgaaaaa agctaaaaag
     1081 ca
//
LOCUS       DAOPUW010000705         1082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_161282_length_1082_cov_3.160662, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000705 DAOPUW010000000
VERSION     DAOPUW010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161282_length_1082_cov_3.160662"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..>1082
                     /locus_tag="PKJ43_09970"
     CDS             238..>1082
                     /locus_tag="PKJ43_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNZ72940.1"
                     /translation="MDNSKHTDALEYHKSGRPGKIQVVPTKPHSTQRDLSLAYSPGVA
                     IPCLEIEKDAQFAYDYTSKGNLVAVISNGTAVLGLGDIGAIAGKPVMEGKGLLFKIFA
                     DIDVFDIEVNEKDPEKFIQVVKAISPTFGGINLEDIKAPECFTIEEKLKEELDIPIMH
                     DDQHGTAIISGAALINALELVGKKIEEVRIVVNGAGAAAVACANLYIKLGANPKNVIM
                     ADSKGVISVRRTDLNSRKKPFATSLPCDTLAEALNGADVFLGLSVKDVLTQEMVRSMA
                     DDPVV"
BASE COUNT          321 a          212 c          229 g          320 t
ORIGIN      
        1 actgaaaggg ttaattttaa ttttacattt aaaaacgttg aataaagatc agtaaatctc
       61 gtattcttga agttattctg ttgaaaatgt tttaattaca cttgatcatt ctttgttttt
      121 gatcgaaaat tgaacatttg ctcatactat tcgatgtatt caccggaata tttatgcctt
      181 ttcacctcta catttgctcg ctaatttcag ataatttttt taaaccaaaa tataacaatg
      241 gacaattcaa aacacaccga cgcgcttgaa tatcacaaaa gtggtaggcc cggaaaaata
      301 caggtagttc ccaccaaacc tcatagtacc caacgcgacc tttcactggc atactcaccg
      361 ggggttgcta ttccttgtct tgaaattgaa aaagatgctc aatttgctta cgactatact
      421 tcgaaaggaa acttggttgc cgttatttca aacggaacgg cagttcttgg cttaggcgac
      481 attggggcta ttgccggaaa accggtaatg gaaggtaagg ggttgttatt taagattttt
      541 gccgatatcg atgtgtttga tattgaagtt aacgaaaaag atcccgaaaa attcatacag
      601 gtagtgaaag caatttcacc cacttttgga gggattaacc tcgaagatat taaagctccc
      661 gaatgcttta ccattgaaga aaaattgaaa gaggagctcg atataccaat tatgcacgat
      721 gaccagcatg gtactgccat aatttccgga gcagctttga ttaacgctct cgaactggtc
      781 ggtaaaaaga ttgaagaagt gcgtatcgta gtgaatggtg ccggggctgc cgcagtagct
      841 tgcgcaaacc tgtatattaa gttgggtgct aatccgaaaa atgtaattat ggctgacagc
      901 aaaggcgtta tttcagtacg tcgtaccgac ctcaacagcc gtaaaaaacc gtttgccact
      961 tcattgcctt gcgacacgct ggccgaggct ctcaatgggg ccgatgtttt ccttggactt
     1021 tcggtaaaag atgtgcttac ccaggaaatg gtgcgctcaa tggctgacga tcctgtcgtt
     1081 tt
//
LOCUS       DAOPUW010000706         1082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_161369_length_1082_cov_2.146056, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000706 DAOPUW010000000
VERSION     DAOPUW010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161369_length_1082_cov_2.146056"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PKJ43_09975"
     CDS             <1..226
                     /locus_tag="PKJ43_09975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ72941.1"
                     /translation="GFPEDALPRVFNKFYRGTINKTGGLGLGLSIVKGFVEAQKGIIT
                     VKNRETRGAVFTLTVPSEKPDIEHFEINEP"
     gene            223..906
                     /locus_tag="PKJ43_09980"
     CDS             223..906
                     /locus_tag="PKJ43_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ72942.1"
                     /translation="MTGPNTILIIDDEIQIRRLLEITLTANGYKVLQSGTGKDGLIDA
                     ATLHPSLIILDLGLPDAEGIEILKKLREWYSKPIIILSVRQSEDDIVTALDYGANDYL
                     SKPFRSAELLARIRVALRASDNKKEAEILHFGTLCIDLPNHAVRKNDEILKLTSTEFS
                     LLSLLAKNQGRVLTHQYILKEIWGYSYIEQTQYLRVFVAQLRKKVEDNPSKPTLLVTE
                     SGIGYRFGE"
BASE COUNT          341 a          215 c          221 g          305 t
ORIGIN      
        1 tggttttcct gaggatgctt taccccgcgt ttttaataaa ttctacagag gcacaatcaa
       61 taaaacagga ggattggggt tgggcttatc tatagtaaaa ggttttgttg aagcccagaa
      121 aggaataatc accgtaaaaa acagagaaac cagaggcgca gttttcaccc ttacagttcc
      181 ttctgaaaaa ccagatattg aacatttcga aataaacgaa ccatgaccgg accaaatacc
      241 atactaatca ttgacgatga aatacaaatc aggcgtttgc tggaaattac cctaactgcg
      301 aatggatata aagtcttaca gtcgggcacg gggaaagacg ggttaattga tgcagccaca
      361 ttgcatccat cgcttattat tctcgactta ggtctcccag atgccgaagg catagagatt
      421 ttgaaaaaac taagagagtg gtactcaaaa cctataataa tcctttctgt cagacaatcg
      481 gaagatgaca ttgtaaccgc cttagattat ggggcaaacg actaccttag caaaccattc
      541 cgaagtgccg aattactggc tcgtattagg gtcgcactca gggcctctga caataagaaa
      601 gaagcagaga ttttacattt tgggactctc tgcattgatt taccgaacca tgccgttcgt
      661 aaaaatgatg aaatattgaa acttacttca actgagtttt cattgctcag cctgttggca
      721 aagaatcaag gccgcgttct cacccatcaa tatatcttaa aagagatttg gggatacagc
      781 tatattgaac aaacacaata cttaagggtt ttcgttgccc aattacgaaa aaaagttgaa
      841 gataacccat cgaaacccac cctgttggtt actgagtcag gcattgggta tcgatttgga
      901 gaatagcaaa cgaagtgttt cttttgccat gctaatttgt tcactatgtc gatctcatct
      961 tttgccggaa tccttattgg tattggtgca tttctcataa taggccttct tcacccggtt
     1021 gttatcaaag gtgaatacta tttcggaata aaaatatggc catggtttct atttggagga
     1081 at
//
LOCUS       DAOPUW010000707         1074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_163298_length_1074_cov_2.482826, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000707 DAOPUW010000000
VERSION     DAOPUW010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163298_length_1074_cov_2.482826"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PKJ43_09985"
     CDS             <1..340
                     /locus_tag="PKJ43_09985"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNZ72943.1"
                     /translation="LLLDVVLECCGQQEALDTAIELIKPGGKLVVVGIPEFDRWSFSA
                     DLVRRKEIMIVNIRRQNHCTEETLNMLADGKIDGSAMISHRFPFTKVHKAFDLVANYR
                     DGVMKAMIDF"
     gene            505..>1074
                     /locus_tag="PKJ43_09990"
     CDS             505..>1074
                     /locus_tag="PKJ43_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNZ72944.1"
                     /translation="MKINPENVPFKTLSNGAKIPVIGLGTFGSDNYSNDQIAQAVKFA
                     IELGYRHIDCASVYGNEKEIGEVLSELIGNGTVKREELWITSKVWNNMHGEGDVIKSC
                     KQSLADLQLNYLDLYLVHWPFPNYHAPGCDGDARNPDSKPYIHENYMVAWRQMEQLVE
                     MGLVKNIGTSNMTIPKMKLLLNDARIKPVV"
BASE COUNT          338 a          187 c          253 g          296 t
ORIGIN      
        1 tttgttgctt gatgtggttc ttgaatgctg tgggcaacaa gaagcgttgg atacagccat
       61 cgagttgatc aaacctggtg gaaaattggt tgtggtgggg attcccgaat tcgatcgttg
      121 gagtttttca gccgatttgg tccgcagaaa ggaaatcatg attgtcaata ttcgccggca
      181 aaatcattgt accgaagaaa cgctcaacat gttggccgat ggtaaaattg atggcagtgc
      241 tatgatttcg caccgttttc cctttactaa agtgcacaaa gcgttcgatt tggtggccaa
      301 ctaccgagat ggggttatga aagcaatgat agatttttaa aaaatacaaa cacgaagtca
      361 ctaagacaca aatagatgat tttcaaaaga taaagaatgt tgaacaagaa gctacaaaat
      421 agtaatagtt tatttttaag aagatgaaag agaacataaa aactttgtgc cttagtgtct
      481 ttgtgttaaa aaaaacaaaa taaaatgaaa ataaatccag aaaacgtgcc ctttaaaacg
      541 ttatcgaatg gggcaaaaat acctgttatt gggttgggaa cttttggttc cgacaactac
      601 tcgaacgacc aaattgcaca ggccgttaaa tttgccattg aactgggcta tcgacatatc
      661 gactgtgctt cggtgtatgg caacgaaaag gaaattggtg aagttctttc tgaattgatc
      721 ggaaacggaa ccgtaaagcg tgaagaactt tggattactt cgaaagtttg gaacaatatg
      781 catggtgagg gagatgtgat caaatcgtgt aaacaatcct tggccgattt gcagctcaat
      841 tacctcgacc tgtacctggt gcattggcct ttcccgaact accatgcgcc cggctgcgat
      901 ggcgatgccc gtaatcctga ttctaagccc tatattcatg aaaattatat ggttgcctgg
      961 aggcagatgg agcagttagt cgaaatgggc ttggtaaaaa acattgggac ttcgaatatg
     1021 accattccga aaatgaaatt gttgttgaac gatgccagga ttaaacctgt ggtc
//
LOCUS       DAOPUW010000708         1072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_163741_length_1072_cov_2.868240, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000708 DAOPUW010000000
VERSION     DAOPUW010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163741_length_1072_cov_2.868240"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PKJ43_09995"
     CDS             <1..697
                     /locus_tag="PKJ43_09995"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNZ72945.1"
                     /translation="FKEKLSFGSPLVLPQFSVLDPTVVASLPPRQIGNGIVDAFVHVI
                     EQYLTYPVDAKVQDRIAEGVMLTLVEEGPKVLANPANYDAAANFMWAATMALNGIISR
                     GVPEDWTTHNIGHEITALHGVDHARTLAIVLPGVMNVMRESKKQKIIQLGQRVWGISE
                     TDENKAIDLTIAKTVEFFELMGLQTRLHEYKIPVLTIDEIVERMEERGSVNIGENKLV
                     GSDEIRKILKDRF"
     gene            847..>1072
                     /locus_tag="PKJ43_10000"
     CDS             847..>1072
                     /locus_tag="PKJ43_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72946.1"
                     /translation="MENNNRFETTLKELLALLGKQSSNLDINKISEACHFAYEILGET
                     SWESGKPIIYHSMSVARIVATDIGLGTDPVI"
BASE COUNT          349 a          223 c          217 g          283 t
ORIGIN      
        1 attcaaagag aaactatcgt ttggttctcc attggtacta cctcaatttt ctgtactcga
       61 tccaacagtt gttgcttctc ttcctcccag gcaaattggc aacggaatag tcgatgcttt
      121 cgttcacgta atcgaacagt atcttaccta tccagtcgat gccaaagtgc aagatcgtat
      181 tgctgaagga gtaatgctca cactggttga agaaggcccc aaggtacttg ccaaccctgc
      241 caattacgat gctgccgcta acttcatgtg ggctgcaacc atggccctta acggaattat
      301 cagccgagga gttcccgaag attggacaac tcataatata ggccatgaaa ttactgccct
      361 tcacggtgtc gaccatgcgc gaacattagc aatcgtactt ccaggagtaa tgaacgttat
      421 gcgtgaatct aaaaagcaga aaattattca gttagggcaa agagtttggg gtatctctga
      481 aaccgacgaa aacaaagcaa ttgatttaac tattgctaaa actgttgaat tctttgaatt
      541 aatgggccta caaaccaggc ttcacgaata caaaattcca gttctcacca ttgacgaaat
      601 tgttgaacga atggaagaac gaggttctgt gaatattgga gaaaacaaac tggtaggtag
      661 tgacgaaatc aggaaaatcc ttaaagaccg gttctaacga atctgaataa ataagcaaaa
      721 tcctgtttcc catgagacag gatttttttt ttactgaaca ccgaaagaac accataatac
      781 gttgaaagct caataaattt ctatttttgt gaaaatgggc gcacctttcg aaaaacagaa
      841 aacgctatgg aaaacaataa tcgtttcgaa accacactga aagagctgtt ggcacttctc
      901 ggaaaacaaa gcagtaatct agatatcaat aagatatcag aagcatgcca ctttgcctac
      961 gaaattctgg gagaaaccag ttgggaatcg ggtaagccaa ttatatatca ctcaatgtca
     1021 gttgcccgaa ttgttgctac tgatataggc cttggaaccg accctgttat tg
//
LOCUS       DAOPUW010000709         1071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_164009_length_1071_cov_2.779528, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000709 DAOPUW010000000
VERSION     DAOPUW010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164009_length_1071_cov_2.779528"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PKJ43_10005"
     CDS             <1..342
                     /locus_tag="PKJ43_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72947.1"
                     /translation="SQSDSWGTTCIGIDETNKMLFFAKKPDVQPVKISLSEVTQCRVI
                     PENYKNGDSKTGQPSAQHIYLELALSKGLPPKMMFEFYNLVQDFNEFDGRYHLASKWQ
                     KIITTYINSKQ"
     gene            474..>1071
                     /locus_tag="PKJ43_10010"
     CDS             474..>1071
                     /locus_tag="PKJ43_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNZ72948.1"
                     /translation="MNLTADNILLTGSILLFISLFAGKTSSRFGVPILVLFLAIGMLA
                     GSEGIGGIYFDNPKSTQFIGIIALNFILFSGGFDTDWKAIKPILWQGITLSTVGVLIT
                     AVTLGLFVWAVTDFTIYEGLLLGSIVSSTDSAAVFSILRSKGLALKGNLRPTLELESG
                     SNDPMAYVLTIAFTGLVINPNAGLASVIPLFFTQIIIGG"
BASE COUNT          304 a          217 c          212 g          338 t
ORIGIN      
        1 tcccaatcag atagttgggg tactacttgt attggcattg acgaaaccaa caagatgctt
       61 tttttcgcaa agaaaccgga tgttcaacca gttaaaattt cgttaagcga agtgacccaa
      121 tgccgggtta ttccagaaaa ttataaaaat ggagactcga aaacagggca accctcggcc
      181 cagcacattt atctggaact agctctatca aaaggactgc ctccaaaaat gatgttcgaa
      241 ttctacaatc tggtacaaga ttttaacgaa tttgacggac gctatcattt agcatcaaaa
      301 tggcagaaaa taataacaac ttacattaac tcaaaacagt agttgtaata gattaaaggg
      361 tctatctttt cagcaagact ctttaatctt ttatatctgt tcagcaaaaa aagattagag
      421 catatgcttt ttatgctgtt ttttcttatt ttgagaaaca caaaattcta acaatgaatt
      481 taacagccga caatatatta cttacaggat ctattttgct gtttatttcg ctctttgcgg
      541 gaaaaacatc gagccgcttt ggggtaccaa ttcttgtgct tttcttggcc attggcatgt
      601 tagctggctc agagggtatt ggaggcattt atttcgacaa tcctaaatcg acacaattca
      661 ttggcattat tgcactaaac ttcatcctgt tttcaggagg ttttgacacc gactggaaag
      721 caattaagcc cattctttgg cagggcatta cactttccac tgtgggagtg ttgatcactg
      781 ctgttacgct agggcttttt gtttgggcag ttaccgactt caccatttac gaggggcttt
      841 tgcttggttc aatcgtttcc tcaaccgatt cggcggctgt tttttccata ttacgttcca
      901 aagggctggc attaaaagga aatcttcggc ctacattgga acttgaaagt ggaagtaacg
      961 accctatggc ttacgtgctt accattgcat ttacgggtct tgtaataaac ccaaatgccg
     1021 ggctggccag cgttattcct cttttcttca cccagattat tattggagga g
//
LOCUS       DAOPUW010000710         1063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_166012_length_1063_cov_3.003968, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000710 DAOPUW010000000
VERSION     DAOPUW010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166012_length_1063_cov_3.003968"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PKJ43_10015"
     CDS             <1..255
                     /locus_tag="PKJ43_10015"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR/HmsC family protein"
                     /protein_id="HNZ72949.1"
                     /translation="IEPVNIIFVSNAHFDDIKEVSTKVGNHCTLIVSERTGGANRGAG
                     VSIIYNEKLAKLELEINKGYMKRNSLNVNDQLYSVASTVY"
     gene            269..>1063
                     /locus_tag="PKJ43_10020"
     CDS             269..>1063
                     /locus_tag="PKJ43_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72950.1"
                     /translation="MKVKFSISKKLFLGFCSIIGLFIISSVVTFIILGRNDAINRKLA
                     EQNTPSVNGLIELQNLITESKLLIKNWVYIDKHTDTPDKLRLMELHSTLYNNAVSTLS
                     TLVQNWKPEDKETFEKITSLITTKLFQEHKQVMSMLNSFESYNDFLVLMDVEGMVEDG
                     GSIIATTDEIINSLSQLIESQQGEATAAYSRIESSASAFRIFIIIGGLIVVTIGVLVM
                     YFLTNAIIHSVNTASSTISKLSEGHLQSDFSVTGNDEFAKLLFDLKE"
BASE COUNT          353 a          213 c          203 g          294 t
ORIGIN      
        1 atcgaaccag taaatattat atttgtatcc aatgcccatt tcgacgacat aaaagaggtt
       61 tcaacaaaag ttgggaatca ctgcacacta attgtcagcg aacgcacagg cggagccaat
      121 aggggagccg gggtaagcat tatttataat gaaaagttgg caaaactgga attagaaata
      181 aataaaggct acatgaaacg caattcgctg aatgtgaacg atcaactata cagcgtagca
      241 tcaaccgttt attaaaatgt taaacgccat gaaagtaaaa ttttccattt cgaaaaagct
      301 attcttagga ttttgttcta tcattgggct attcattata tcatccgtag taactttcat
      361 cattctcggc agaaacgatg ccataaatcg aaaacttgcc gaacagaata ccccctcggt
      421 aaacggcttg attgaacttc aaaaccttat taccgagtcg aaattactta tcaagaattg
      481 ggtatatatt gacaagcaca ctgatacacc ggacaagctt aggctgatgg aattacacag
      541 cactttgtac aacaatgctg tttcaaccct ttctacgctt gtccaaaact ggaaacctga
      601 ggataaagaa acttttgaaa aaattaccag cctcataacc acaaaactct ttcaggagca
      661 taaacaggta atgtccatgc tcaactcctt tgaaagctac aacgattttt tagttttaat
      721 ggatgtagaa ggaatggtgg aggatggagg ttcaatcatc gctacaacgg atgaaataat
      781 caattcgctt tcgcaactta tcgaaagcca gcagggggaa gccacggccg catattcaag
      841 gattgagtca tccgcttccg catttagaat ttttatcatt attggcggat tgattgtggt
      901 taccatcggg gtactggtta tgtacttttt aacaaacgcc ataatccatt cggttaatac
      961 tgcatccagc actatttcaa aactatctga aggtcatctg caatcagatt ttagcgtaac
     1021 cggaaacgat gagtttgcca aactgctttt cgaccttaaa gag
//
LOCUS       DAOPUW010000711         1059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_167000_length_1059_cov_2.891434, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000711 DAOPUW010000000
VERSION     DAOPUW010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167000_length_1059_cov_2.891434"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /gene="ilvC"
                     /locus_tag="PKJ43_10025"
     CDS             <1..902
                     /gene="ilvC"
                     /locus_tag="PKJ43_10025"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNZ72951.1"
                     /translation="RDNGFNVIVGQRKGGKTWEKAVADGWVPGETLFDIEEAAEKGTI
                     IQYLLSDAAQIEVWPRIKPYLTAGKALYFSHGFGITFKDRTGIVPPSDIDVIMVAPKG
                     SGTSLRRLFLEGRGLNSSYAIFQDATGKAFDRVVALGIGVGSGYLFETTFEREVYSDL
                     TGERGTLMGAIQGLLSAQYEVLRAHGHSPSEAFNETVEELTQSLMPLFAENGMDWMYA
                     NCSTTAQRGALDWMGPFHDATKPVFEKLYNEVASGREAQRSIDANSRPDYRERLEEEL
                     KALRESEMWRTGAVVRKLRPENN"
BASE COUNT          304 a          209 c          253 g          293 t
ORIGIN      
        1 tacgggacaa tggttttaat gtcattgtgg gacaacgcaa aggtggcaaa acttgggaaa
       61 aagctgttgc agatggctgg gttcccggcg aaacactttt cgatattgaa gaagctgcag
      121 aaaaaggaac cattattcag tatttgcttt cggatgcagc acaaatagaa gtttggccgc
      181 gtattaaacc gtatctgacc gccggcaaag ccttgtattt ttctcacgga tttggcatta
      241 cttttaaaga tcgtacaggc atcgttcccc cttctgatat cgacgtaata atggtagcac
      301 caaaaggttc gggaacttct ttgcgtcgct tgttcctcga agggcgtgga ttaaattctt
      361 cttacgctat ttttcaggat gctacaggaa aggcctttga cagagttgta gcactcggca
      421 ttggggttgg ttcgggttat ttattcgaaa ctacttttga aagagaagtg tattccgacc
      481 ttaccggtga acgcgggact ttgatgggag ctattcaggg acttctgtcg gctcaatatg
      541 aagtgctcag ggcacacgga cattcacctt cagaagcttt caacgaaaca gtggaagaat
      601 tgacgcaatc gctgatgcca ctttttgctg aaaacggcat ggactggatg tatgccaact
      661 gttccactac cgctcagcgt ggtgcgttgg actggatggg accgttccac gatgctacca
      721 aaccggtatt tgaaaaactt tataacgaag tggcttcagg tcgtgaagca caacgttcca
      781 tcgacgctaa ttcgcgtccc gattacaggg aacgactcga agaggaatta aaagcattgc
      841 gcgaaagcga aatgtggaga acaggtgctg tggtcagaaa actacgcccc gaaaacaatt
      901 gatgctgatt tgatcagtaa aattatttaa gaaaaaagcc ttgctctttg tcaaatttca
      961 gagcaaggct ttttagttgg tagcaacaag aaataattta tgtcgtcgtt ccttaaattt
     1021 attctattcg actaaatatc aataaaatag ttataaaaa
//
LOCUS       DAOPUW010000712         1058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_167226_length_1058_cov_3.177468, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000712 DAOPUW010000000
VERSION     DAOPUW010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167226_length_1058_cov_3.177468"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..>1058
                     /locus_tag="PKJ43_10030"
     CDS             192..>1058
                     /locus_tag="PKJ43_10030"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNZ72952.1"
                     /translation="MLFNSIEFLIFFPIVTLLFFVLPHKYRWFHLLAASCIFYMFFIP
                     QYIFILLVTIVIDYVAAIWIAKTDGKKRKRYLVISVVSTCAVLFVFKYFNFFNDNMLL
                     LSQKMGFYYPKNVVSFILPIGLSFHTFQSLSYVIEVYRGHQKPEKHFGIYSLYVMFYP
                     QLVTGPIERPQNLLVQLRQEKTFSYENLSHGLRLILWGLFVKMVVADNIGAYVDQIYA
                     KPESFNSLSILNGLFFYSFQIYCDFFGYSTIAIGCAKTMGYNLMDNFKNPYLAQNIRE
                     FWQRWHISLSTWF"
BASE COUNT          275 a          209 c          207 g          367 t
ORIGIN      
        1 gtgcagctaa actcaacctg aaaattactg aggttatcgt gcgttaccgc gaccgtgagt
       61 atggatcaac gcagattagc cgctttagcc atggatggtt actgattaaa atgagcttgt
      121 ttgctgcccg aaaaatgaaa ttccgttaga aatttatagt caaatactta tacaataaag
      181 atacattacc catgctgttt aattccatcg aattcctgat cttttttccc attgttaccc
      241 tgctgttctt tgtgttaccc cataaatacc ggtggtttca tttgttggcg gccagttgca
      301 ttttctacat gttttttatc ccccagtata tttttattct cttagttact attgttatcg
      361 attatgtagc ggctatctgg attgccaaaa ccgatgggaa aaagcgaaag cgctatctgg
      421 tgattagtgt ggtttctact tgtgccgtgc tgtttgtttt caaatacttc aacttcttta
      481 acgataacat gctgttgctg agccagaaaa tgggcttcta ttatcccaag aatgttgtgt
      541 cctttatcct tcccataggc ctttcgtttc atacttttca aagcttaagt tatgtaattg
      601 aagtgtaccg tggtcaccaa aaaccagaaa agcatttcgg catatactcg ctttatgtaa
      661 tgttttatcc tcaattagtt acggggccta ttgagcgccc gcaaaacctg ctggttcagt
      721 tgcgccagga aaaaacattc agttacgaaa acctttctca tggactgcgg cttattttgt
      781 ggggcttatt cgtaaaaatg gttgttgccg ataacattgg ggcttatgtc gaccaaatat
      841 atgctaaacc agagagcttc aactccttaa gcatattgaa tgggttgttt ttctattctt
      901 tccagatata ttgcgatttt tttgggtatt caaccattgc cattggatgt gctaagacta
      961 tgggttacaa tttaatggac aattttaaaa acccatattt agcacaaaat attcgcgagt
     1021 tttggcagcg ctggcatatc tcgctgtcaa cgtggttt
//
LOCUS       DAOPUW010000713         1057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_167555_length_1057_cov_2.270459, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000713 DAOPUW010000000
VERSION     DAOPUW010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167555_length_1057_cov_2.270459"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PKJ43_10035"
     CDS             <1..785
                     /locus_tag="PKJ43_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNZ72953.1"
                     /translation="IKYIFSEIEAGKLRIPGFHVDWAKPTFSIVRFLMYAFMFILIFP
                     YLPGSESRVFQGVSVFLGLLVSLGSSSAIANMVAGLVITYMRLFKIGDRIKIGEVTGN
                     VVEKTMLVTRLRTSNNESITIPNSAILSGNTTNYSALAATEGLILSTKVTISYQTPWR
                     TVQKALLEAAGRTSNLLHNKKHFVIQSALDQFHAVYQLNVYTSEGNAFAPIYNELHQN
                     IQDVCAEYGIEIVSPQFLIQYESAPSITPPVDTPEDGKKGKK"
BASE COUNT          311 a          240 c          197 g          309 t
ORIGIN      
        1 ttatcaaata cattttttct gagatagaag cggggaagct cagaataccc ggtttccatg
       61 tcgattgggc taagcccact ttcagcatag taaggttttt aatgtatgcc ttcatgttca
      121 tcctcatttt tccctatctg cccggctccg agtcaagggt ttttcaagga gtttcggtat
      181 ttttaggatt attggtatcg ctgggttctt ccagcgccat tgccaacatg gttgccggat
      241 tggtaattac ctatatgcgg ctattcaaaa ttggcgatag gattaaaatt ggagaggtaa
      301 ccggcaacgt agtcgaaaaa accatgctgg taacccgcct tagaacatca aacaacgaga
      361 gtattaccat tcccaattct gccattcttt caggaaacac caccaactac tcagctctgg
      421 ctgccaccga agggttaatt ttgagcacca aggttacaat cagctatcaa acgccctggc
      481 gcaccgttca gaaagcgctc ctcgaagccg ccggccgtac ttcgaatctg ttacacaata
      541 aaaagcattt cgtcatacaa tcggcattag accaatttca cgctgtttat caattgaatg
      601 tttacacatc ggaaggcaat gcttttgctc ccatctacaa tgaactccat cagaatattc
      661 aggatgtgtg cgccgagtat ggcatcgaaa tagtgtcgcc acagttttta attcaatatg
      721 aaagcgcccc ttccattacc ccacctgtgg atacgccgga agatggcaaa aaaggtaaaa
      781 aatagaaaat aacctcaatt ctattttcct gcgatttggc aaagtattgt tgccattgtt
      841 ttgtggattc tttgtattac taataaggac aatatccata ataaaaacaa ccccttaagc
      901 aatgaacttt ctccactatc atattgcaaa gcagagaagt aatactctct gctctcaaac
      961 gattatagct ttttaaattt taactttgca ttcacttaaa ccaacacaaa gtataatgaa
     1021 aacaagacat ttcttcgtat cgttgcttgc cggggtt
//
LOCUS       DAOPUW010000714         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_168334_length_1054_cov_2.407407, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000714 DAOPUW010000000
VERSION     DAOPUW010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168334_length_1054_cov_2.407407"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PKJ43_10040"
     CDS             <1..820
                     /locus_tag="PKJ43_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ72954.1"
                     /translation="FEIETGRYFSPYELYAGKNVILLGHEVASELFTGFNPVGQSVKV
                     RGHKAEVIGVIKKEGASPIGNYSHDEIVLIPVSFAKQIIDLRWMDTFIMVKPVENVPN
                     IELKDELRGILRSFHRLKPIEDDDFSLNELGLLKQGIDSIFVSINLGGWIIGGFSILV
                     GGFGIANIMFVSVKERTGIIGIQKALGAKNYFILLQFLYESILLSVMGGLIGLFVVFI
                     GSLLASGFIEFNITLTFGNIVLGLAISATIGIVSGFAPAWSASKLNPVEAMNAN"
BASE COUNT          267 a          186 c          255 g          346 t
ORIGIN      
        1 gttcgaaata gaaacggggc gttacttttc gccctacgaa ttgtatgcgg gcaagaatgt
       61 gattttgctg ggacatgaag tggcatcgga gctatttacg ggcttcaacc ccgttgggca
      121 aagcgtaaaa gtaaggggtc ataaggccga ggtgattggc gtgataaaaa aggagggagc
      181 gtctcccatt ggcaactaca gccacgatga aatagtattg attccggtta gttttgcgaa
      241 acagataatt gacctaagat ggatggatac gtttataatg gttaaacctg ttgaaaatgt
      301 acccaacatc gaattgaagg acgaattacg tggcattctc cgttcgtttc atagattgaa
      361 acccattgaa gacgacgatt tctcgctaaa cgagcttggc cttctcaaac agggtattga
      421 cagcattttt gtgtccataa atttgggagg atggattatt ggtggttttt ccattctggt
      481 gggtggtttt ggcatagcca atattatgtt cgtctcggta aaagagcgaa ccggtatcat
      541 tggcattcaa aaggcgttgg gagctaaaaa ttattttata ctcttgcaat ttctttatga
      601 gtcgattttg ctttcagtta tgggagggct cattgggtta tttgtggtat ttattggttc
      661 cctgctcgca tcaggtttta tcgaatttaa tattaccctg acatttggaa atattgtatt
      721 gggattagct atttccgcca ccattggcat tgtctccgga tttgccccgg catggtcggc
      781 atcaaagtta aaccccgtgg aggccatgaa tgctaattaa cgtgagtttg acctaagccg
      841 cctttactgt tttacacgtg cgcacgtgcg ttggaacagg cacactcttt ggcaagtttt
      901 ggttacagtc tgattcgtgc ggtttggcta tgtgtgcctg tgaagcgttg atattacttt
      961 gcactttcaa tgatttcatt tcttattctg aatatttgaa atttagattc aactaataaa
     1021 tgcaactttt tgagggcatg aattaatttt tttc
//
LOCUS       DAOPUW010000715         1050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_169309_length_1050_cov_3.349749, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000715 DAOPUW010000000
VERSION     DAOPUW010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169309_length_1050_cov_3.349749"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(285..>1050)
                     /locus_tag="PKJ43_10045"
     CDS             complement(285..>1050)
                     /locus_tag="PKJ43_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNZ72955.1"
                     /translation="KTYDIPDDVGGRYSVLTPVGLLPIAVAGINIRELVKGAQDMAKA
                     TAPEVKFDENIACQYAAARHALYTEGKKIEILVNYQPKLHFMAEWWKQLYGESEGKNG
                     KGIFPASVDFSSDLHSMGQYIQEGERILFETVISVAKPERELLIPEDKANLDKLNFLA
                     GKNIDYVNKMAELGTQIAHVDGDVPNIRIEIERLNEYFVGQLIYFFEKACGISGYLIN
                     VNPFDQPGVEAYKKNMFALLEKPGFEAETKMLKSRL"
BASE COUNT          274 a          216 c          216 g          344 t
ORIGIN      
        1 ttatggtttg tttttactat ttgccaatat acacataata tttcttaatt cacaaccttg
       61 tttagactta cctcacattc gtgattcatg tacaactaag tggcagcgtt ggatgtgtca
      121 ggcctttgag agtggggtat agccttgttt tgaggctttg tttagaaaat cgatttctta
      181 ttatcctaaa agaaaaaccg accttaagaa atattgattt cctgaggtcg gtattatata
      241 taatcctgtt tatttcgcta aattacagga ataatgacaa tgaattatag ccggcttttc
      301 agcatttttg tctcagcttc aaaccccggt ttttcaagca gggcaaacat atttttctta
      361 taggcttcaa ctccaggctg gtcgaatggg tttacattaa tgaggtaacc acttatacca
      421 catgcctttt cgaagaagta aattagttgg ccaacgaaat attcgttcag tcgttcaatt
      481 tcgatgcgaa tgttgggaac atctccgtcg acatgggcaa tttgtgtacc caattcagcc
      541 atcttattca cataatctat gtttttccct gccaggaagt tcaacttgtc gaggttggct
      601 ttatcttcag ggataagcag ttcgcgttct ggttttgcaa cagaaattac agtttcgaac
      661 aatatacgtt cgccttcttg gatgtattgt cccatagagt gcaagtcgga cgagaaatcg
      721 accgatgcag gaaaaatacc cttaccgttt ttcccctcac tttcgccgta gagctgtttc
      781 caccactcgg ccatgaaatg aagttttggt tgataattga ccaaaatttc aattttcttt
      841 ccttcggtgt acaaggcgtg tcgtgcggca gcgtattggc aggcaatgtt ttcgtcaaat
      901 ttaacttcgg gagctgtagc ttttgccata tcctgagcgc cttttacaag ttccctgata
      961 ttgattccgg caacagcaat cgggagcaag cctacagggg taagtacaga gtaacgcccg
     1021 ccaacatcgt cggggatatc ataggttttg
//
LOCUS       DAOPUW010000716         1045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_170655_length_1045_cov_2.689899, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000716 DAOPUW010000000
VERSION     DAOPUW010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170655_length_1045_cov_2.689899"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..877
                     /locus_tag="PKJ43_10050"
     CDS             110..877
                     /locus_tag="PKJ43_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNZ72956.1"
                     /translation="MNLQLRNPLIFLDLETTGINIASDRIVEIALLKINPDGNEDEKL
                     MRINPEMPIPEQSSRIHGIYDEDVKNCPTFREVAKTLANFMEGCDLAGFNSNRFDIPL
                     LAEEFLRAGVDIDMKKRKFIDVQAIFHKMEKRTLEAAYKFYCQKDLTDAHSAEADTLA
                     TYEVLKAQLDMYPDLENDVEWLASFSSHTRNVDFAGRIVLDEKDVEVFNFGKYKGRSV
                     AEIFEKEPSYYAWMMNGDFPLYTKKVLTNIYLKLRNK"
BASE COUNT          316 a          216 c          226 g          287 t
ORIGIN      
        1 tcgacgacga agacgtgctg atgctcctga tgccaatgat gataaattca taaagccaaa
       61 accgagaaag cgctgaacga aacggcgctt ttttttttaa tatacaacta tgaacttaca
      121 attaagaaac ccgttaattt ttctcgacct cgaaactaca gggatcaaca ttgcctctga
      181 tcgtattgtt gaaatagccc tgttgaaaat aaatcccgat ggtaacgaag atgaaaagct
      241 tatgcgcatt aaccctgaaa tgcccattcc ggaacaatca tcgcgaatcc atggtattta
      301 cgacgaagat gtaaaaaatt gccccacctt cagagaagtg gccaaaacac tggcaaattt
      361 catggaaggc tgcgatttgg cgggttttaa ttcaaaccgt ttcgacattc cactgttggc
      421 cgaagaattt ttgcgggcag gagtcgacat cgatatgaaa aaaagaaaat tcatcgacgt
      481 tcaggctatt ttccacaaaa tggaaaagcg tacccttgaa gctgcttaca agttctactg
      541 ccaaaaggat ttaaccgatg cccatagcgc tgaggccgac acccttgcca cctatgaggt
      601 gctaaaggct cagctggata tgtatccaga tttagagaac gatgtggagt ggcttgcctc
      661 tttcagctcg catactcgta acgtggattt tgctggcaga atagtgcttg acgagaagga
      721 tgtagaagtg tttaactttg gtaagtacaa gggccgttct gttgccgaga tttttgaaaa
      781 ggagcctagc tactacgctt ggatgatgaa tggcgacttt cccctttaca ccaaaaaggt
      841 gttaactaat atttacctta agctaaggaa taaatagtgg cgtgtgtggc tatggagtta
      901 ctgcgttaat ccattaatcc ttattcgaat acctattatg aagataattt gtataggacg
      961 aaattactct gagcacgcaa aggagatgaa ctctccagtt cctgacgttc ccgtattttt
     1021 tattaagccc gatacagcct tactt
//
LOCUS       DAOPUW010000717         1044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_170958_length_1044_cov_2.235592, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000717 DAOPUW010000000
VERSION     DAOPUW010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170958_length_1044_cov_2.235592"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1044)
                     /locus_tag="PKJ43_10055"
     CDS             complement(<1..>1044)
                     /locus_tag="PKJ43_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ72957.1"
                     /translation="AKNSNNLPPEATFAVRNIENPVFLINFICSNADIKVEEKQNLLQ
                     FDHLRERGIQAISYLVRELQMIELKRDIQSKVKTDMDQQQREYLLHQQMKTIQDELGG
                     NPIEKEIEELKEKAKTKKWNEEVAALFEKEADKLRRMNPAAGEYSIQLSYVQTLLDLP
                     WNEYSEDNFDMKHAEQVLEEDHYGLELVKERILEHLAVLKLKNDMKSPIICLYGPPGV
                     GKTSLGKSVARALGRSYMRMSLGGLHDESEIRGHRKTYIGAMPGRIIQNIKKAKTSNP
                     VFILDEIDKVSADYHGDPASALLEVLDPEQNSEFHDNYLEIEYDLSKVMFIATANTLN
                     TISQPLRDRMELI"
BASE COUNT          237 a          219 c          225 g          363 t
ORIGIN      
        1 tcaataagtt ccatacggtc gcgaagaggt tgactaatcg tattcagcgt gttggcagtg
       61 gcaatgaaca ttactttcga caagtcatat tctatttcaa gatagttgtc gtgaaattcg
      121 ctgttttgtt ccggatctaa aacttcgagc agtgccgatg ctggatctcc atgataatcg
      181 gcagaaactt tatcaatttc gtcgagaata aaaaccgggt tcgatgtttt ggcttttttt
      241 atgttttgaa taatccggcc gggcatagct ccgatatagg tttttctgtg accgcgaatt
      301 tccgattcgt cgtgaagccc gccaaggctc atacgcatgt aactacgtcc cagtgctcgt
      361 gctaccgatt ttcccaacga tgttttccca actcccggag ggccgtaaag acaaatgatg
      421 ggcgatttca tatcgttctt aagtttcagt acggcaaggt gttcaagaat gcgttctttc
      481 accagttcga gcccgtaatg atcttcttct aatacttgct cggcatgttt catgtcgaaa
      541 ttgtcttctg aatattcgtt ccagggcaag tcgagcaggg tttgcacata cgaaagttga
      601 attgaatatt ctcccgctgc agggttcata cggcgaagtt tgtcagcctc cttttcgaac
      661 aaagcagcaa cttcttcatt ccattttttt gttttagctt tttccttcag ctcttcaatt
      721 tctttttcaa tagggtttcc tccaagttcg tcctgtatgg ttttcatttg ctggtgcagc
      781 aagtattcgc gctgctgctg atccatgtct gttttcactt tggattgtat atcgcgcttt
      841 agttcaatca tttgaagttc gcgaaccaag tatgatatgg cctgtatacc tctttcccgt
      901 aagtggtcga actgtaaaag gttttgcttt tcttctactt ttatatcggc atttgagcaa
      961 atgaagttga taagaaagac cggattttca atgtttctta ctgcgaatgt tgcttctggg
     1021 ggtaggttat tcgagttttt tgca
//
LOCUS       DAOPUW010000718         1038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_172422_length_1038_cov_3.855544, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000718 DAOPUW010000000
VERSION     DAOPUW010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172422_length_1038_cov_3.855544"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..149
                     /locus_tag="PKJ43_10060"
     CDS             9..149
                     /locus_tag="PKJ43_10060"
                     /inference="COORDINATES: protein motif:HMM:NF013835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72958.1"
                     /translation="MLGAQIASIHNLAFYLWLVGEARKHIVTGDFSQWKEHMIQQLKT
                     RV"
     gene            157..>1038
                     /locus_tag="PKJ43_10065"
     CDS             157..>1038
                     /locus_tag="PKJ43_10065"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNZ72959.1"
                     /translation="MKLFKKFDLYIMKKFLGTFFFTILLMISISVVFDLTEKIDEFIQ
                     NQAPLKAIIFDYYLNFIPYFANLYSSLFLFISVIFFTSKLAFNSEIIALMSSGISFKR
                     LLVPYLTSAFVVFLLMLVLGHFVIPPANKGMIDFKFTYVKKKSTNRDKNIHRQIEPDV
                     YIYIERYDASNDIGRKFSIERFENGKLVSKLNSEHIRWNRERNIWTINNYTIRDLKGN
                     TETITTGSSKDTTLAMVPEEFKTKKEDVDIMNYFELKDFITQQRLRGVSSLEEYEIER
                     HRRTSVPFSVFILTIIGA"
BASE COUNT          320 a          200 c          201 g          317 t
ORIGIN      
        1 caaaagaaat gcttggtgcc caaattgcca gcattcacaa cctggctttt tacctctggc
       61 tggtgggcga agcccgaaag cacattgtta ccggagattt ctcacaatgg aaagagcata
      121 tgatacagca attaaaaacc cgagtataaa ttattgatga aacttttcaa gaaattcgac
      181 ctctacataa tgaagaaatt cctcggaaca ttcttcttta ccattttgct tatgatttcg
      241 ataagtgtgg tgttcgattt gactgaaaag attgatgaat ttattcagaa ccaagctccg
      301 ctgaaagcta ttattttcga ttactacctg aattttatcc cctattttgc aaacctttac
      361 agcagcttgt tcctttttat ttcagtaatt ttctttactt cgaagttagc tttcaattca
      421 gaaattattg ccttaatgag cagcgggata agttttaaac gcttgctggt accctatctc
      481 acttcggctt ttgtggtctt tttactcatg ttggtattgg gccattttgt aattcctccg
      541 gcaaataagg ggatgatcga ttttaagttc acttatgtga aaaagaaatc cacgaatcgt
      601 gataaaaata tccatcggca aattgagccc gatgtatata tttacataga gcgttatgat
      661 gcctcgaacg acattggtcg caaattttcg atcgagcgtt tcgagaacgg caaattggtt
      721 tcgaaactta attctgaaca cattcgttgg aaccgggagc gaaacatttg gacaattaac
      781 aattatacaa ttcgcgactt aaaaggaaat actgaaacga taacgaccgg gagttccaaa
      841 gatactacat tggcaatggt gcccgaagag ttcaaaacca agaaagaaga tgtagacata
      901 atgaactatt tcgaattgaa agatttcatt acacagcaac gcctgagagg ggtaagttcc
      961 ctcgaagagt atgaaattga acggcaccga cgaacttctg ttccattctc ggtgtttata
     1021 ctcaccatta taggtgct
//
LOCUS       DAOPUW010000719         1036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_173086_length_1036_cov_2.188583, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000719 DAOPUW010000000
VERSION     DAOPUW010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173086_length_1036_cov_2.188583"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1036)
                     /gene="sprA"
                     /locus_tag="PKJ43_10070"
     CDS             complement(<1..>1036)
                     /gene="sprA"
                     /locus_tag="PKJ43_10070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNZ72960.1"
                     /translation="ATYPLDAPVPSNIKTEIEYDPSTASYILRTYLGNIEIATPFRMT
                     EQEYLDYSGRQEMQRYWYEKNSSAQQNVEDKFSVTDMKFDIGPADKIFGPGGVQIRTQ
                     GSAEISMGFKTNKIDNPALTERMRKSTILDFDEKIQMNVNGKVGDKVNFNMNYNTESS
                     FDFDQKLLKLNYKGKEDDIIKNIEIGNVSMPLNSSLITGSTALFGVKTDLQFGKLSIS
                     AVASQQQSQTQTVSSKRGAQTTDYEIGIDDYDENRHFFLGHYFRNNFENAMSKLPFVT
                     SGVTITRVEVWVTNKRGNYDQARNIVAFMDLGETQRLDNHYWNVLSSTSLPQNKANSL
                     YDEVTSIPNVR"
BASE COUNT          283 a          206 c          202 g          345 t
ORIGIN      
        1 gcgaacatta ggaatagaag taacttcatc ataaagagaa ttggctttgt tttgtggtaa
       61 agatgtggat gaaagcacat tccaataatg attgtcaagt cgctgtgttt cccccaaatc
      121 cataaatgca acaatgtttc ttgcctgatc ataatttcca cgtttgttgg ttacccaaac
      181 ttcaacacgt gtaatggtaa cacctgaagt tacaaatggg agcttgctca tggcattttc
      241 aaaattgttt ctgaaatagt gtcccaaaaa gaaatgccgg ttttcatcat aatcatcaat
      301 accaatttcg taatcggtag tttgtgctcc tctttttgaa cttacggttt gcgtctgcga
      361 ttgttgctga gaagctacgg cgctaatgct taacttgcca aactgcaaat cggttttaac
      421 accgaacagt gccgtgcttc cggtaatgag agaactgttt aacggcatgc tgacattacc
      481 aatttcaatg tttttaatga tatcgtcttc cttgccttta taattcagtt ttaacaattt
      541 ctggtcaaaa tcgaaactcg actcggtatt gtaattcata ttgaagttca ccttgtcgcc
      601 aaccttgcca tttacattca tctgaatttt ctcgtcaaag tcgagaatag tacttttgcg
      661 catccgctca gtcaatgccg gattgtcgat tttgttggtt ttgaaaccca tagaaatttc
      721 tgcagaacct tgtgttctga tttgaactcc accgggacca aatattttat cggcagggcc
      781 aatatcgaat ttcatgtccg taacactgaa tttgtcttca acgttttgtt gtgcagaaga
      841 atttttttcg taccaataac gttgcatttc ttgccttccg gaataatcga gatattcctg
      901 ttccgtcatt cgaaacggag tagctatttc gatgtttccc aaataagtac gcaaaatata
      961 actggctgtt gaggggtcgt actcaatttc cgttttaata tttgaaggaa cgggtgcgtc
     1021 gagagggtaa gtagca
//
LOCUS       DAOPUW010000720         1034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_173704_length_1034_cov_1.454545, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000720 DAOPUW010000000
VERSION     DAOPUW010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173704_length_1034_cov_1.454545"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PKJ43_10075"
     CDS             <1..981
                     /locus_tag="PKJ43_10075"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HNZ72961.1"
                     /translation="NVYAKRAARAHMIFNVFGVLWMLFVFPYFIRAIGYSIVYFGGVS
                     PFESPQSTPVALSLFHTSFNVMNVLVQVMFTPAIVKVVTFMVPHRKEEDEEFRLRHLE
                     IGLLSTVELSLLQAYKEINIYATRTYRMYGFFKELFTETNEKKFKELFDRIEKYEEIS
                     DRVEVEIATYLTKVSTSELSEESSKRMQSMLKIISDIESMSDSNYTLAKTIRRKRKAN
                     IWFNQEIRDNLNQMFSLVDDAFHVMLGNLQEDYTKINLGPAYEAEEKINQYRNKLRKQ
                     HLKNVEEDKYKYQAGVIYHDLFSEAEKLADFIINISEDIAEMSKPVKQTV"
BASE COUNT          327 a          196 c          228 g          283 t
ORIGIN      
        1 aacgtatatg ccaaaagagc agcgcgtgca cacatgatat ttaatgtgtt cggcgttttg
       61 tggatgctgt ttgtattccc ctactttatt cgagccattg gatattcaat agtttatttc
      121 ggtggcgttt cgccattcga atcgcctcag agcacacccg ttgccctgtc tctgtttcac
      181 acatcgttca atgtaatgaa tgttttagta caggtaatgt ttactccggc cattgtaaaa
      241 gttgtaacgt ttatggtacc ccatcgcaaa gaagaggatg aggagtttag gttgcggcat
      301 ctggaaatcg ggttgctttc aactgttgaa ttatcattgc ttcaggcata caaagagata
      361 aacatctatg ccacacgcac ctacagaatg tacggttttt ttaaagagct ctttactgaa
      421 accaacgaga aaaagtttaa agagcttttc gatcgcatcg aaaagtatga agagataagc
      481 gatagggttg aggttgaaat agccacatat ttaaccaagg tctccacaag cgaattgagc
      541 gaagagagct caaagcgaat gcaatccatg ctgaaaataa tcagcgatat cgaaagcatg
      601 tccgatagca actataccct ggccaaaacc atcaggcgta aacgtaaggc caatatatgg
      661 ttcaaccagg agattcgcga taatctcaac caaatgttca gcctggttga tgatgctttt
      721 catgtaatgt tagggaatct tcaggaggat tataccaaaa ttaatttggg gccggcatac
      781 gaagcggaag agaaaatcaa tcagtataga aacaaactca ggaaacagca tttaaagaat
      841 gttgaggagg ataaatataa gtatcaggca ggtgtaattt accacgacct cttttccgaa
      901 gcagaaaaat tggccgattt tattatcaat atttctgagg atattgctga aatgagcaag
      961 cccgttaagc aaacggtttg atgaaaaggg aagtttaaac cgatttccct tttagggcct
     1021 cggctcctga aact
//
LOCUS       DAOPUW010000721         1026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_175846_length_1026_cov_1.645726, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000721 DAOPUW010000000
VERSION     DAOPUW010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175846_length_1026_cov_1.645726"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PKJ43_10080"
     CDS             <1..404
                     /locus_tag="PKJ43_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72962.1"
                     /translation="GKKLIKAWLYGELPNDLSFQLKVECPFEVSQILKRIYSEPFPNR
                     FLASFVPFLEKNSGHPFVNQMVDDAFEDLIRHHLLRYSDFFRLPIGVVGSVGSIFGKN
                     LSKLIEAKGGKISRILHYPIDEMVRFHIENT"
     gene            complement(401..>1026)
                     /locus_tag="PKJ43_10085"
     CDS             complement(401..>1026)
                     /locus_tag="PKJ43_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72963.1"
                     /translation="IQGIWFSCKIICQYFSKSVLINDTISRSEKYSKEQLSTWGKKGY
                     YQYYYGLTIGLIVAQKYHSLFTLLNRFSNPAPKGSLNSVEKALILFYEYSKWLEKPEY
                     DPETMLELQTYMSEMPQWNSYIPLIVGNSWHAMYSLSLGKVERAYELIQKCIEISNLA
                     GYLVFEVRMLKTLNSLLLSLGEPKKAHDCEDFAKGLVNKSGIDFDLL"
BASE COUNT          297 a          200 c          218 g          311 t
ORIGIN      
        1 ttggtaaaaa attgattaaa gcatggttat atggagaact accgaatgac ctgtcgtttc
       61 aacttaaggt agaatgccct tttgaagtaa gtcagatctt aaaaagaata tattccgagc
      121 catttcctaa tcgttttctg gcgagttttg tccccttcct tgaaaaaaat tcaggacatc
      181 cgtttgtaaa ccaaatggtg gatgatgcgt ttgaagatct tatcagacat catttgctga
      241 gatacagcga tttttttcgt ttgcccatag gcgtggtagg ttcagtgggt agtatttttg
      301 gtaagaattt gtcaaaactt atagaagcca aaggcggaaa gattagccga attctacact
      361 accccatcga tgagatggta cgcttccaca ttgagaacac ctaaagcagg tcgaaatcaa
      421 tccccgattt attgaccagc cctttggcaa aatcctcgca atcgtgggcc tttttcggct
      481 ctcctaacga taggagcaag gagttcaatg ttttcaacat cctcacttca aaaacaaggt
      541 aacctgcaag gttgctgatt tcaatgcatt tttggataag ttcgtaggcc ctttccacct
      601 tgccaagcga gagagagtac atggcatgcc aactatttcc tacgataagt ggaatatagc
      661 tattccactg gggcatttcg ctcatatacg tttggagttc aagcattgtc tccgggtcgt
      721 attcaggttt ctccagccat ttagagtatt cgtaaaaaag gatgagggct ttttccactg
      781 agtttaaact tccttttgga gccggattgg aaaacctgtt gagaagggta aagagcgaat
      841 ggtacttttg tgcaacgatt agtccaatgg taagcccata gtagtattgg taataacctt
      901 tcttgcccca agtagataac tgctctttgg aatatttttc agaccgggaa attgtatcgt
      961 taatcaaaac ggatttagaa aagtattgac agatgatttt acacgaaaac caaatcccct
     1021 gtatgt
//
LOCUS       DAOPUW010000722         1025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_175935_length_1025_cov_3.351546, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000722 DAOPUW010000000
VERSION     DAOPUW010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175935_length_1025_cov_3.351546"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            470..>1025
                     /locus_tag="PKJ43_10090"
     CDS             470..>1025
                     /locus_tag="PKJ43_10090"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNZ72964.1"
                     /translation="MHEHPANAKEKEHHLRIRRVLSYIQDNLTSELTLEKMACVANIS
                     PFHFQKLFIKYVGESPKQYVLRLRLERSAHQLVLYPEQSVFEISLQSGFSSASTFGRA
                     FKNYFGITPDEFRKVSNFDNSKNGTLIHKNSKEHDEHFHKFWSIDFDGNETIGQATGE
                     GIHVVKLSSLKIAYKMIIYNFKHIE"
BASE COUNT          339 a          205 c          185 g          296 t
ORIGIN      
        1 attaacgatc ggcactaata tacgatgatg cctgatctgc tggtcaacta ccctaaatat
       61 gttagtggac cagcagatca ggcatcatcg tatattagtg ccgatcgtta ataacgaata
      121 tcgttaacca attcataagg tggcatttaa acaacaaatg ccaccttttt tttgactttt
      181 tgtctttttc tgaaatagct tgacttaaaa aacaagttat tctgaaaaat ttgactcaga
      241 accctacaga tacgatgaaa ggatccatcc aggagaaagt agatttaaag aagtatgaga
      301 ttgttgctaa atgggtatat gcactgatat gccttaatgc aactttgttg aaaacgtatt
      361 ttaacactcg tttcaaatac aatgcaaaaa tataacataa ggtaagccac taacttttgc
      421 attatatttt ttcatatttt catactgaat tttaagcaag tcacaactca tgcacgaaca
      481 tccggcgaac gcaaaggaaa aggaacatca tcttcgtatc aggcgggtac tttcttatat
      541 tcaggataac cttacatcgg agcttacgct cgaaaaaatg gcctgtgttg caaatatttc
      601 cccatttcat tttcaaaagc ttttcatcaa atatgtgggc gaatctccca aacaatacgt
      661 gctgcgcctg cggttagaac gctcggccca ccaactggtg ctttaccccg aacaatcggt
      721 tttcgaaatt tcgctgcaaa gcggattctc ctcggcatcg acctttggaa gggctttcaa
      781 aaactatttt ggcatcacac ccgatgagtt ccgtaaagtc tcgaattttg ataatagcaa
      841 gaatggcaca ttgattcaca aaaacagcaa agaacatgac gaacatttcc acaaattttg
      901 gagcatcgat tttgacggaa atgaaaccat cggacaggcc acaggcgaag gcattcacgt
      961 ggtgaagtta agttctttga aaattgcata taaaatgata atatataact tcaaacatat
     1021 tgaga
//
LOCUS       DAOPUW010000723         1023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_176516_length_1023_cov_2.901860, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000723 DAOPUW010000000
VERSION     DAOPUW010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176516_length_1023_cov_2.901860"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1023
                     /locus_tag="PKJ43_10095"
     CDS             <1..>1023
                     /locus_tag="PKJ43_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72965.1"
                     /translation="RTFSYGDNLSLFVNHPEKEFSEYRIVNPIVGKKYFQRFEYTEPP
                     KDAFLKEKPDSCFRIFVMGSSTVVGFPYDNNIMFSRILHEWLRETYPGKKIEVVNTAI
                     TAINSFTLLDYMPQILRQEPDAILFYAGHNEFYGAFGSGSSEASVQNPAFIRLHLKLL
                     NFRVYQLTRNIISSVAESVSKTKTAAAKRGTLMSRMAKDADITFGSKKYNEGIENYRK
                     NLDMMLAMANAKKVPVFMSDLVSNIRDLKPFSSQPTSSLKPADFYFDLARQQEAAGDF
                     NAAKENYTKARDYDCVRFRASSDINNCIVELSKKYKNVRFVPTLSLFEANSPNRLVGN
                     NLVTEHV"
BASE COUNT          302 a          203 c          220 g          298 t
ORIGIN      
        1 ccgtacattc tcgtatggcg acaacctttc tttgtttgtc aatcatcccg aaaaggaatt
       61 tagtgagtat cgtattgtaa atccaatagt tggaaagaaa tattttcagc gttttgaata
      121 taccgaaccc cctaaagatg catttttaaa agaaaaaccc gacagttgtt ttcggatctt
      181 tgtaatggga agctcaactg tggttggttt cccgtatgat aacaacataa tgttttcccg
      241 tattttacat gaatggttaa gggaaacata ccccggaaag aaaattgaag ttgttaatac
      301 tgccattacg gcgatcaata gttttacgtt gctcgattac atgccgcaaa ttcttcgcca
      361 agaaccagat gcaattttgt tttatgcagg gcataacgaa ttctatgggg cttttggttc
      421 ggggtcaagc gaggcatctg tccagaatcc ggctttcatt cgacttcacc ttaagttgct
      481 taattttagg gtgtatcagt taactcggaa tattatttca tcggtggcag aatcggtttc
      541 gaagacaaaa actgctgctg caaagcgggg tactctcatg agccgtatgg caaaagatgc
      601 cgatataact tttggcagca aaaaatataa tgaagggatt gagaactacc gtaaaaacct
      661 cgatatgatg ctggctatgg ccaatgcaaa gaaagttccg gttttcatga gcgatctggt
      721 cagtaatatt cgcgatctga aacctttcag ctctcagcct accagcagtt taaagcctgc
      781 cgacttttac ttcgacctgg cacgtcagca ggaggcagcc ggcgatttta atgcggcaaa
      841 agaaaactac accaaagccc gtgattacga ttgtgtgagg ttcagggcat cgagcgatat
      901 aaataattgt atcgtcgaac tttcaaaaaa atacaaaaac gtacgatttg tgcctacttt
      961 aagtcttttc gaggctaatt cacctaaccg ccttgttgga aataatctgg ttacagagca
     1021 tgt
//
LOCUS       DAOPUW010000724         1022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_176884_length_1022_cov_2.250259, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000724 DAOPUW010000000
VERSION     DAOPUW010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176884_length_1022_cov_2.250259"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1022)
                     /locus_tag="PKJ43_10100"
     CDS             complement(<1..>1022)
                     /locus_tag="PKJ43_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNZ72966.1"
                     /translation="ALAATDSSSEISYGALLDNALRFYSLLSRYSFSTRHVAVYLPNG
                     TEALSVIVSLLTAGVAYAALDPETPSDRNKQIADTGDFDYLITSELFVERAASFFSSD
                     KILVYSDLIKNDPVAFELVRDVQPEDHSVLFFTSGSTGIPKCAVHTHLSIFNGLKDSL
                     DILKVRKNDSFDMIAPLSFAASINVFLALQRGCPIHFFDVKRQGIHGYVKFLRERKIS
                     ITVMTATVFRAVCRIVQTAGSGRFSSFRLVLMALKYARKYKLNTTQVRKSKIKAISEI
                     VKVAGISNLPSLRMINLVGEPVVPADIELFKSITGKDAELLNVYGASETRTISIKIFK
                     HSDNDV"
BASE COUNT          331 a          222 c          201 g          268 t
ORIGIN      
        1 tttacatcat tatcagaatg cttaaaaatc tttattgaga tagttcgggt ttcactggct
       61 ccgtaaacat tcagcaattc agcatccttc cctgtaatcg acttaaagag ttcaatatcg
      121 gcaggaacaa ccggctcacc aaccaaatta atcattcgca atgaaggcaa atttgaaata
      181 ccggcaactt ttacaatctc gctgatagct tttatcttgc ttttccgtac ctgtgtagtg
      241 ttcaatttgt acttacgggc atatttcaat gccatgagca caagccggaa ggaagaaaaa
      301 cgccccgaac ctgcagtttg aactattcgg caaacagccc tgaacacggt ggcagtcatt
      361 acggttatgc taatttttcg ttcgcgcaaa aatttcacat atccatgaat tccctgcctc
      421 ttaacatcga aaaaatggat tgggcaacct cgctgcaatg caagaaaaac attaatacta
      481 gcagcaaaag aaaggggagc aatcatgtcg aaggaatcgt ttttacgcac ctttaaaata
      541 tccagcgaat ctttaagccc attaaaaata cttaggtgag tatgaacggc acatttggga
      601 atacctgtcg atcccgaggt gaaaaaaagc acagaatggt cttctggctg aacatctcgc
      661 accaattcga atgcaacggg atcgtttttg attaaatctg aataaaccag tattttatcc
      721 gatgaaaaaa aagaagcagc ccgctcaaca aataattcag aggtgattaa ataatcaaaa
      781 tcaccggtat cagcaatttg tttatttcta tcgcttgggg tttcaggatc caatgcggca
      841 taggccaccc ctgctgtcaa caacgaaaca atcactgata atgcctcggt tccattgggc
      901 aaataaacag ctacatgtct ggtgctgaat gaatagcgcg aaagcaggga ataaaagcga
      961 agtgcattat cgagtaaggc cccatagctt atttcacttg aagaatcggt tgcagccaag
     1021 gc
//
LOCUS       DAOPUW010000725         1020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_177527_length_1020_cov_1.778238, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000725 DAOPUW010000000
VERSION     DAOPUW010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177527_length_1020_cov_1.778238"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..>1020)
                     /locus_tag="PKJ43_10105"
     CDS             complement(1..>1020)
                     /locus_tag="PKJ43_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72967.1"
                     /translation="NVISSSFVVVVDFLLIYAGLFFTTNFWANNFKSTIDYYPQIFLL
                     GVLPMYILTWLTGAYLSGGYEKPFRLSGIVKGILASTFVIAFVYAFLPETWRFSRAII
                     LFGAIVSMASLTITRLFYNMVKHGRLSFDEQMPKRWLIVGDETESSRAIDLLKKYEEP
                     ELTYTLSTANGVKGKSALLSIDEVVFCSKTVPFRTIIDSIEETNGKQNFKILNPDSNA
                     LIGSNSKNTAGDLLAEDKRLNLSKHLAQRKKRMLDIVLCGLLPFTFKSSVYSNLWAVL
                     SGKKTWAGYITNEAKSLPFNKEAVFPIVKGKNIMESDAQLLNELYAKNYTLTEDMKII
                     FSNLH"
BASE COUNT          309 a          205 c          200 g          306 t
ORIGIN      
        1 ctaatgcaaa tttgagaata taatcttcat atcctctgtc agtgtatagt ttttagcgta
       61 caattcattc agtaattgcg catcactctc cataatattt ttgcctttca cgatcgggaa
      121 aactgcttct ttgttgaagg gtagcgattt tgcttcgttg gttatatacc cggcccaggt
      181 tttctttccc gagagtacag cccaaagatt gctatatacc gatgatttga aagtgaatgg
      241 taataggccg cacagcacaa tatcgagcat gcgttttttg cgttgcgcta agtgtttgga
      301 aaggttaagc cgtttatctt ctgccaatag atcaccggct gtatttttag agttactccc
      361 aatgagagca ttcgaatcag gatttaaaat cttgaagttt tgttttccat ttgtttcttc
      421 aatactatcg atgatggttc tgaaaggcac agttttgctg cagaatacaa cttcatcaat
      481 gctgagcaaa gccgatttcc cttttactcc attggcggtt gacaaagtat aggtaagttc
      541 aggctcttca tactttttca acaaatcgat ggcgcggctg ctttcggttt catcgcccac
      601 aatcaaccag cgtttcggca tttgttcatc aaaggatagg cgaccgtgtt ttaccatgtt
      661 gtagaacaag cgggtaatag ttaaactagc catggaaact atagcgccaa acaaaataat
      721 agcacgagaa aatcgccatg tttccggcaa gaaggcatat acaaaggcaa ttacaaaagt
      781 actggctaaa atgcctttta caatacctga aaggcgaaat ggtttttcgt aaccgcccga
      841 taaataggcc ccagtaagcc aagtaagaat atacattggc aatacaccta acaaaaatat
      901 ttgcgggtaa tagtcgatag tgcttttgaa attattagcc cagaaattcg ttgtaaagaa
      961 caagcccgcg tatatcaata aaaagtctac tactactaca aatgacgagg aaatcacatt
//
LOCUS       DAOPUW010000726         1016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_178453_length_1016_cov_3.663892, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000726 DAOPUW010000000
VERSION     DAOPUW010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178453_length_1016_cov_3.663892"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1016
                     /gene="hflX"
                     /locus_tag="PKJ43_10110"
     CDS             <1..>1016
                     /gene="hflX"
                     /locus_tag="PKJ43_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNZ72968.1"
                     /translation="QEAEKVVLVGLITNNQDEAHALEYIAELEFLADTAGASVVKKFF
                     QRIPNPHPVTFVGSGKLDEIVNFVKENEIDGIIFDDELSPLQLRNIERVAECKVLDRT
                     NLILDIFAKRAQTAYAKAQVELAQLQYTLPRLTRMWTHLERQRGGIGMRGPGETQIET
                     DRRIILDKISRLKDQLKKIDQQMVTQRKNRGKLVRAALVGYTNVGKSTLMNLLSKSEV
                     FAENKLFATLDTTVRKMVIGNLPFLITDTVGFIRKLPHGLVESFKSTLDEVREADILL
                     HIIDISHKGFEEQLAVVQQTLEEINASDKPTIYVFNKIDAFTYVEKDEDDLRETTSLN
                     WTLD"
BASE COUNT          320 a          236 c          214 g          246 t
ORIGIN      
        1 tcaggaagca gaaaaagtcg ttttagtggg gttgataacc aacaaccaag atgaggctca
       61 cgcgctggaa tacatagcag aacttgagtt tcttgccgat acggctggag cttcggtcgt
      121 gaaaaaattc tttcagcgta tacccaaccc ccaccctgta acctttgtcg gttcaggaaa
      181 actcgatgaa atcgtcaact ttgttaaaga aaacgaaatt gatggaatca tcttcgacga
      241 tgaactttca ccccttcaac ttcgtaatat cgaaagagtt gctgaatgta aggtcctcga
      301 caggacgaac ctcattctcg atatattcgc aaagcgtgca caaacagctt acgcaaaagc
      361 ccaggttgaa ctggctcaat tacaatatac cctaccccgc ctcacccgca tgtggactca
      421 cttggaacgg caacgtgggg gtattggcat gcgcggcccc ggtgaaactc agattgaaac
      481 cgaccgccgt attattcttg acaaaatttc aaggctgaaa gatcaactga aaaaaattga
      541 tcagcaaatg gttacccaac ggaaaaacag gggaaaactg gttagggctg cactggtggg
      601 ctataccaat gttggaaaat cgacactgat gaacctgcta agcaaatcgg aagtatttgc
      661 cgaaaataag ctctttgcca ccctcgatac taccgtaaga aaaatggtaa tcggcaacct
      721 gcccttcctt attaccgaca ccgtaggttt catacgcaag ctcccgcacg gccttgtcga
      781 atcgttcaaa tcgaccctcg atgaagtacg cgaagccgat attctgctcc atattatcga
      841 tatttcgcac aaaggtttcg aagagcaatt agctgtggtt caacaaactc ttgaagaaat
      901 taatgcatct gacaaaccaa caatttatgt attcaacaaa atagatgcgt ttacttatgt
      961 tgaaaaagac gaagatgacc taagggaaac gacttcttta aattggactc tcgatg
//
LOCUS       DAOPUW010000727         1013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_179409_length_1013_cov_2.268267, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000727 DAOPUW010000000
VERSION     DAOPUW010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179409_length_1013_cov_2.268267"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PKJ43_10115"
     CDS             <1..519
                     /locus_tag="PKJ43_10115"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNZ72969.1"
                     /translation="TISKNSEDNYTIWKYVGMAPDGKTTRASVIQNSANWIVYRYAEV
                     LLMKAEALSQLQRFTEAAELLNEIRDRAGVPKLALPSSSVAYEDAILEERALEFAFEG
                     KRWFDLVRMGRRNNYARKDKLVEIIISNVPSTQKRILGSKLTNPQGWYMPIYKYELER
                     NLNLVQNSYYKE"
     gene            524..>1013
                     /locus_tag="PKJ43_10120"
     CDS             524..>1013
                     /locus_tag="PKJ43_10120"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNZ72970.1"
                     /translation="MRKLLFIIAVISGALFFQACDKSEVPDAGFKDMERLSIYDYIVE
                     NDSLFSKYQQVLVAAGLDKTMSAYNPNGDGYTMFLPTNAAIDEFVATNDRYSSFDDML
                     KDKDFLGSLVRYSVVEAAILTNDFPFGALPEFNLSGQYLMIGFDGGIYSVNNEAPVSA
                     ELK"
BASE COUNT          301 a          190 c          233 g          289 t
ORIGIN      
        1 acaataagta aaaatagcga agacaattat acaatttgga agtatgtggg tatggctccc
       61 gatggcaaga ctacccgtgc aagcgtaata caaaatagtg ccaactggat tgtttaccga
      121 tatgccgaag tattgctgat gaaagccgaa gcactttctc aattacagcg ttttaccgaa
      181 gctgctgaat tgttaaatga aattcgtgac agagccggtg taccaaaatt agctttaccc
      241 agttcttctg ttgcttacga agatgctatt cttgaagaga gggctctcga gtttgcattt
      301 gaagggaaaa gatggtttga ccttgttcgt atgggccggc gaaacaacta tgcccgaaaa
      361 gataaacttg ttgaaattat tatttcgaac gtgccatcga cacaaaaacg aattttggga
      421 tcgaagctca caaatccgca aggatggtat atgccaattt ataaatatga attggaaaga
      481 aatttgaact tagtgcaaaa ctcatattat aaagagtaag acaatgagga agttactatt
      541 tattattgcc gtgattagcg gggccttgtt ttttcaggcc tgcgacaaaa gcgaagtgcc
      601 agatgccggg tttaaagaca tggaacggct atcgatatac gactatattg ttgaaaatga
      661 ttccttgttt tcaaagtatc agcaggtgct ggttgctgct gggctcgata aaactatgag
      721 tgcctataat ccgaatggag acgggtatac tatgttttta cccaccaacg ctgccatcga
      781 cgagttcgtt gccaccaacg accgttattc ttcattcgat gatatgctga aagacaaaga
      841 ttttcttgga tctttggttc gctatagtgt ggttgaagca gccattttga ctaacgactt
      901 cccatttggg gcactgcctg aatttaacct ttccgggcaa tacctgatga ttggtttcga
      961 tgggggaatt tatagcgtga acaacgaagc ccccgtttct gccgaactga aga
//
LOCUS       DAOPUW010000728         1011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_179883_length_1011_cov_3.247908, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000728 DAOPUW010000000
VERSION     DAOPUW010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179883_length_1011_cov_3.247908"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PKJ43_10125"
     CDS             complement(<1..696)
                     /locus_tag="PKJ43_10125"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNZ72971.1"
                     /translation="MKNIKSLLLTIVVLALGFGAYAQKVLTIDEAMALALKNNFDISV
                     SKSEAAISATNNTAGAAGMLPSINLTGSGNYALKQTGSKASENEEYTNQQNGIATLNA
                     GAELSWTIFDGGKMFVAKSKLNEIEALGEIRYKEQVLQTLADVISAYYNVVKQKQQLA
                     AIEQIIKYNSERVTIAETAFKTGSKSKTDLLQAKIDLNVNQQNAINQKTAIETARREL
                     ARKLVTDSNFDVSS"
     gene            complement(742..>1011)
                     /locus_tag="PKJ43_10130"
     CDS             complement(742..>1011)
                     /locus_tag="PKJ43_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNZ72972.1"
                     /translation="AEFEAASSRLRPILMTSLATVFGALPIALALGSGAESRIPLGVV
                     VVGGILFSLLLTLFVIPVMYIVMTSKKPRKANSELMLELEKEGIK"
BASE COUNT          286 a          233 c          184 g          308 t
ORIGIN      
        1 agaagaaaca tcaaaattgc tatcggtaac cagctttcgg gccaattcgc ggcgggctgt
       61 ctcaatcgcc gttttttggt taatagcatt ttgctgattt acgttaagat cgattttcgc
      121 ctggagcaaa tcggttttac ttttcgaacc tgtcttgaaa gcagtttcag caatagtaac
      181 ccgttcactg ttatacttta taatctgctc tatggctgct aattgctgct tttgcttgac
      241 tacattgtaa tatgcgctaa taacatcagc caaggtttgc agcacttgct ctttatacct
      301 tatttcacct aacgcttcaa tctcgttcaa ttttgatttg gcaacaaaca tctttccccc
      361 gtcaaaaata gtccacgaaa gttcggctcc ggcattaagg gtagcaatac cattctgctg
      421 gtttgtatac tcttcgtttt cagaggcttt acttccagtt tgttttaacg cataattacc
      481 cgatcccgtt aagtttatcg aaggaagcat tccggcagca cctgccgtgt tgttggttgc
      541 cgatatagca gcctcacttt ttgaaactga aatatcgaaa ttgtttttta acgccagggc
      601 cattgcctca tcaatggtga gaactttttg tgcataagca ccaaagccaa gcgctaaaac
      661 gactattgtc aacaacaatg attttatatt cttcatgtga aacacttata atactattca
      721 ataacaaaag gttatgtcaa attatttaat cccttctttt tcaagctcga gcatcaattc
      781 gctgttcgcc tttcttggct ttttactggt catgacaata tacatcaccg gaataacaaa
      841 caacgtgagc aagagtgaga aaagaattcc cccaacaaca acaacaccaa gaggaatgcg
      901 gctttcagca cccgaaccca aggccagtgc aataggcaat gccccaaaaa cagtggccag
      961 cgaagtcatt aaaattggcc gtaaccttga tgatgcagct tcaaattcgg c
//
LOCUS       DAOPUW010000729         1009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_180581_length_1009_cov_2.090147, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000729 DAOPUW010000000
VERSION     DAOPUW010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180581_length_1009_cov_2.090147"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PKJ43_10135"
     CDS             <1..258
                     /locus_tag="PKJ43_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72973.1"
                     /translation="GANYNYSISSSGGGTPVTGSSTIATATDTISGIDTTPLTDGTLT
                     ISVTLTDTAGNAGTAATDTVIKDIVAPYITSVTATNGTYEP"
     gene            341..601
                     /locus_tag="PKJ43_10140"
     CDS             341..601
                     /locus_tag="PKJ43_10140"
                     /inference="COORDINATES: protein motif:HMM:NF025668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HNZ72974.1"
                     /translation="MAEEVVQAYVHRINPTVEWPYKELKAFTRVSLSPGENKLVTLEI
                     PVKNLMYWNETAQKWDNDLCDIELQVGASAGDVKLKKKVSLR"
BASE COUNT          379 a          199 c          194 g          237 t
ORIGIN      
        1 ggggcaaatt ataactactc aatctccagt tcgggcggag gaacacctgt cactggttcg
       61 agtacgattg caacagcaac ggatactatc agcggtattg ataccacccc gctgaccgac
      121 ggaacattaa ccatttccgt taccctgacc gatacagcgg gcaacgcggg aacagcagcc
      181 accgatactg tgataaagga cattgtggca ccctacatca catctgtaac agcaaccaac
      241 ggaacatacg aaccttaacc acctgaaaat aaacaaaaat agttttaaag caacagatgt
      301 aataacgctt actttcgacc tgaaaaacac aggaaaggta ttggcagaag aagtggtcca
      361 agcttatgtt catcggataa atccaaccgt tgaatggcct tacaaagaat tgaaagcatt
      421 tactagggta agcctctctc cgggtgaaaa caagcttgtg acactcgaaa taccggttaa
      481 aaacttgatg tactggaacg aaaccgctca aaaatgggac aacgatctat gcgatataga
      541 acttcaggta ggtgcatcgg ctggtgatgt gaagctgaaa aagaaagtat cgctgcggta
      601 atctgttgat aaatttcacg attagagaca acaaaataca gaacaaccaa aaaaccatta
      661 gttatttaat taccaaaaga ttaaatcatt aaaacaacgt gcgaagatgt gtctatttct
      721 ttaataaagc acacttcgca cgtttcattt atataaaatt aaagttgaca attttttatt
      781 tctgacaaaa cataaaagaa aagtacattg agaataatcc gataaagaat ctgaaaaggg
      841 aaaatgaaca acaccaaaca acaataaata atttttcggc tagctttgtt tatatttgtt
      901 caaacaacta ataaaatgaa taaacgagga tttgcaagcg acaataattc aggtgtaagc
      961 ccaagcatac ttaaggcact ggaagatgcc aacgaagggc atgtaacag
//
LOCUS       DAOPUW010000730         1005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_181620_length_1005_cov_3.742105, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000730 DAOPUW010000000
VERSION     DAOPUW010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181620_length_1005_cov_3.742105"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1005)
                     /locus_tag="PKJ43_10145"
     CDS             complement(<1..>1005)
                     /locus_tag="PKJ43_10145"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNZ72975.1"
                     /translation="LSVTCLGYEKLTVPNIQVISGKECLLELEMAESFETIGEIKVNG
                     HNGKRGEVLNEMAIISARAFTVDETKRYAGSLDDPSRMVSAFAGVTTDPAGLNEIVVR
                     GNSPKGIQWRLEGLEIPNPNHFSHQGNTGGPINALSSNMLSTSDFYTGAFSPEYGNAL
                     SGIFDMKMRTGNNEKHEFTLGVGALGIDVAAEGPFKKGNKSSYNFNYRYSTLSLIDNM
                     GLVDFGGVPRYQDLSFKTNFITKSAGTFSVFGLLGLSDIDVNQTNAENDTVFRGTQQS
                     RFGVIGLNHLIHVNNNSFFKTSIAFSNNGSGVDAESLFSDDKFYESMKRDWNNNTLQV
                     "
BASE COUNT          248 a          235 c          217 g          305 t
ORIGIN      
        1 aaacctgaag ggtgttgtta ttccaatctc ttttcatcga ttcgtaaaac ttgtcatcgc
       61 tgaaaagaga ctctgcatca actcccgatc cgttgttcga aaaggctatc gaggttttga
      121 aaaaagaatt attgttgacg tgaattaaat ggttcaaccc aatcactcca aacctcgatt
      181 gctgcgtgcc tctgaacacg gtgtcgtttt ctgcattggt ttggtttaca tcgatgtcgc
      241 ttaaacccaa aagcccgaat accgagaacg ttccggcact tttggtaata aagtttgttt
      301 taaatgaaag gtcctgataa cggggtactc cgccaaaatc aaccaacccc atgttgtcga
      361 ttaaggacaa cgttgagtac ctgtagttga aattatacga acttttgttt ccttttttga
      421 aaggcccttc ggcagctaca tcaattccaa gggctccgac acccagggta aattcgtgtt
      481 tttcgttgtt cccggttctc attttcatgt cgaaaattcc gcttaacgcg ttaccatact
      541 cgggagaaaa agctccggtg taaaaatcgg aagtcgatag catattgctg cttagcgcgt
      601 taattggccc tccggtattg ccttgatgcg agaagtggtt cgggttgggg atttcaagcc
      661 cttcgagacg ccattgaatt ccttttggac tgttgcccct gaccacaatt tcgttcagcc
      721 cggcagggtc ggtggttacc cccgcaaagg cacttaccat tcgtgagggg tcatcgagag
      781 agcctgcata tcgcttggtt tcatcaacgg tgaaagctct tgcgctaata attgccattt
      841 cgttcagcac ttctccccgc tttccgttgt ggccattcac ttttatttca ccaatggttt
      901 cgaacgattc ggccatttcg agttctaaaa ggcattcttt gccgctaatt acttgaatat
      961 tagggactgt aagtttttca taacccagac aagtgacact taaag
//
LOCUS       DAOPUW010000731         1005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_181657_length_1005_cov_2.825263, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000731 DAOPUW010000000
VERSION     DAOPUW010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181657_length_1005_cov_2.825263"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PKJ43_10150"
     CDS             complement(<1..730)
                     /locus_tag="PKJ43_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNZ72976.1"
                     /translation="MKTAYIVKAYRTAVGKAPKGVFRFKRPDELAAETIEHMMNELPD
                     FDKKRIDDVMVGNAMPEAEQGLNVGRLISLMGLKVTDVPGVTVNRYCASGLETIGMAT
                     AKIQSGMADCIIAGGAESMSFIPMGGYKPTPDYAVAKEGNEDYYWGMGLTAEAVAKQY
                     NVSREDQDAFAFHSHQKALKAQAEGKFDKQIVPITVEQTFINENGKKETKSYTVTKDE
                     GPRADTNIAALGNLKPVFAADGSVT"
     gene            complement(807..>1005)
                     /locus_tag="PKJ43_10155"
     CDS             complement(807..>1005)
                     /locus_tag="PKJ43_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72977.1"
                     /translation="ISDHDKKIANKLAYVMAGGDLSEPTLVSEQYLLDIEREAFLSLC
                     TERKTLERIQFMLTKGKPLRN"
BASE COUNT          244 a          212 c          190 g          359 t
ORIGIN      
        1 cggtaacact tccatcagca gcaaaaactg gttttagatt tcccaatgct gcaatattcg
       61 tgtcggctct tgggccttcg tctttggtta ctgtgtaaga tttggtttct ttttttccat
      121 tttcattgat gaaagtttgc tcgacagtaa tcggaacaat ttgtttgtcg aatttacctt
      181 ctgcttgtgc tttcaatgct ttttgatgtg agtgaaaggc aaacgcatct tgatcttctc
      241 tggaaacgtt gtattgtttc gctacagctt cggcagttaa acccataccc caataataat
      301 cttcatttcc ttcttttgct acagcataat ctggagttgg tttgtaacca cccatcggaa
      361 taaaactcat gctttcggca ccaccagcaa tgatgcaatc ggccattccg gattgaattt
      421 tggcagttgc cattccgata gtttccaagc ctgaagcgca atatctgttg acggttacac
      481 caggtacatc agtgactttc aatcccatta aggaaattaa tcgacccaca ttcaaacctt
      541 gttcggcttc gggcatggcg tttccaacca tcacatcgtc gattcttttc ttgtcgaaat
      601 cgggcaattc gttcatcata tgttcgatgg tttctgctgc gagttcgtcg ggtcttttaa
      661 aacggaacac gcctttgggt gctttaccca ctgcggttct gtatgctttt actatgtagg
      721 ctgttttcat ttgttatttt gaattttgaa ttttaaatgg ttgtcaatcg ttaactgacg
      781 gttcattcaa aatttataat ttataattaa tttcttagcg gttttccttt agtcaacata
      841 aactggatgc gctctaacgt ttttctttcg gtgcacaaac tcaaaaaggc ttcacgctcg
      901 atatctaata aatattgttc agagactaat gtcggttctg ataaatcacc gccagccatg
      961 acataagcaa gtttgttggc tattttttta tcgtgatctg aaatg
//
LOCUS       DAOPUW010000732         1004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Prolixibacteraceae bacterium isolate AMR_MDS_1408
            NODE_181952_length_1004_cov_3.003161, whole genome shotgun
            sequence.
ACCESSION   DAOPUW010000732 DAOPUW010000000
VERSION     DAOPUW010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854613
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Prolixibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Prolixibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales;
            Prolixibacteraceae.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:28:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,033
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 2,014
            CDSs (with protein)               :: 2,014
            Genes (RNA)                       :: 11
            tRNAs                             :: 9
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 4 of 8
            Pseudo Genes (internal stop)      :: 4 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Prolixibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181952_length_1004_cov_3.003161"
                     /isolate="AMR_MDS_1408"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053594"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..454)
                     /locus_tag="PKJ43_10160"
     CDS             complement(65..454)
                     /locus_tag="PKJ43_10160"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNZ72978.1"
                     /translation="MENTKPTPTAAELEILSLLWENQPLTVKEIHEQLAVTKDVGYTT
                     ALKIMQNMTAKGLLRREPNGKNHLYYPVYQKEETRGTLLDRFLDSTFAGSVSGLAMQL
                     LGNKKTSARDLAEIKQIIEQMEKEQPL"
     gene            complement(705..>1004)
                     /locus_tag="PKJ43_10165"
     CDS             complement(705..>1004)
                     /locus_tag="PKJ43_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ72979.1"
                     /translation="SGETAIALPAGTYQLRLAIEGTNCNIDKIIFNSEPVSANSMKID
                     KFEGEYEVISMLGVSRGIVKISNGDVSQLYGMFPKGIYILRKQDGSGEARRIAIQ"
BASE COUNT          260 a          212 c          197 g          335 t
ORIGIN      
        1 gcctgccaga tagcgtgaac aagggtccag cccaacgctt cgatgatttc agggttaagg
       61 agtttcataa gggttgttct ttttccattt gttcaataat ctgtttgatt tctgctaaat
      121 cccgggcgct ggttttcttg ttcccaagaa gttgcattgc caatcccgaa accgagcccg
      181 caaaagtcga gtcgagaaaa cgatcgagta gtgtgccccg tgtttcttct ttttggtaga
      241 ccggatagta caggtggttt ttaccgttgg gttccctgcg gagcaacccc tttgcagtca
      301 tgttctgcat aattttcaac gccgtggtat atcctacgtc ttttgttacg gccaattgct
      361 cgtgaatttc ctttacggtt aaaggttggt tctcccaaag cagggaaaga atctccaatt
      421 cagcagcagt gggtgtgggc tttgtgtttt ccattttcga actacgattt gtgtcgtaca
      481 aatatatacg actcttgtcg tagttgcaag gatttattga attatttttt ttgaaatttt
      541 tatttcacca gtgtaaatca aaataagacc ctgaaagact gattattgaa agttcccaat
      601 ctataaaaaa aacgaggtgt aaaaatcaac tttaacacct cgttttcaaa atatttcaac
      661 ttgtgacaat tattttctta tcacattcgt ctacaaatta catgttactg aattgctata
      721 cgccttgctt ctccgctgcc atcttgtttg cgaagtatgt aaattccctt tggaaacatt
      781 ccatacaatt gagaaacatc gccattgctg attttaacaa ttccgcggct gacacccaac
      841 atagagatta cttcatactc tccctcgaac ttgtcaattt tcattgaatt agcacttact
      901 ggttctgagt tgaagattat cttgtcgata ttgcaattag tgccctcaat ggctaaacgg
      961 agctggtatg ttcctgcagg taaagcaata gctgtttcgc ccga
//
